LOCUS       DAOSTD010000001        49361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_798_length_49361_cov_13.672170, whole genome shotgun sequence.
ACCESSION   DAOSTD010000001 DAOSTD010000000
VERSION     DAOSTD010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 49361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49361
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_798_length_49361_cov_13.672170"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2736
                     /locus_tag="PLI37_00005"
     CDS             <1..>2736
                     /locus_tag="PLI37_00005"
                     /inference="COORDINATES: protein motif:HMM:NF040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HOQ57432.1"
                     /translation="YSYCGYMASGQLIPEVPVKAVVAWMPGDNTARIQQAIDYVSSLE
                     PDAQGFRGTVLLEKGCYEIAGGLTISASGVVLRGSGYMDGQTELLGTGTDRTTMIRVA
                     GKKDYQTGENKEISSYVPTGARAVVVQGHSFKPGDRIRIARPGTQAWIDAMDMNDFGG
                     ESSYIGWKPEDMVLYWERQVKEVRGDSILLDAPLSCALDPEFGGGFVSSFEWPGRISR
                     SGVENLRLRSTYDADNPKDENHRWVAINLDNIEDAWVRRIEFRHFAGSAVFLTENTRR
                     ITVKDCKSIEPISEIGGQRRQTFFTLGGQCLFQRLYSEYGFHDFAVGRCAPGPNAFVQ
                     CYAWMPYNFSGTVDSWSTGVLFDVVSIEGHGIYFSNRGQDGLGAGWTTGNSMMWNCSA
                     SMLACPLPPTAYNWSYGAWGQRSGNAAWNSAGSFVKPHSLYYAQLAARTGKKHPDEDK
                     LMPFDTSSATNPPLDRAQEFVAEARKPALELKDWMDSLTLSNPLTLTYEKSKKNDPSV
                     VWQKKSAGKTSTVSPDPAAWMKLEGGVLVKEGKILTGSRRGVVWWSGNLRRRYLATAR
                     PHITRYAPGRTGTGFTDDLDEMTDNMLRQNVLVTDHNYGLWYDRRRDDHERIMRMDGY
                     VWPPFYELPFSRSGQGTAWDGLSKYDLTQWNVWYWNRLKKYADLADEKGLVLFHQHYF
                     QHNILEAGAHWADFPWRSANNVNETGFPEPVPYAGDKRIFMAELFYDVQHPVRRELHR
                     NYIRKCLDNFAANGSVLQFISEEFTGPLHFVEFWLDVIAEWEAETRQDAKVVLCVTKD
                     VQDKILDDPKRAAIVDVIDIKYWRPLPGGGYTTLLSDLNLAPRQQTRAGSTRIPGEGA
                     ADMPGRVYETILSLRETFPDKAVLYSAGGSPQEEWAAFMAGASLCNLPARLPEQLL"
     assembly_gap    2737..2836
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2837..5778
                     /locus_tag="PLI37_00010"
     CDS             <2837..5778
                     /locus_tag="PLI37_00010"
                     /inference="COORDINATES: protein motif:HMM:NF040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HOQ57433.1"
                     /translation="GYMASEAEIPSVPVKALVPAMEGDATEHIQAALNYVSSLKPDQN
                     GFRGAVLLEPGRYEVGTTLWINASGVVLRGSGYGPGGTEIIANYRDRTTLIRFKGVDN
                     RSTGTCYTIADESLPVGTMQMVLPGHRLKAGDHIRISLPARSSQQAASGQNASGQEAA
                     PARMRNTPSMQWEREITSVDGDKVCLDAPLTYEISTRNGSADVCLLGWPGRIRQVGLE
                     NLRLTSVYDQSNPKDENHAWIAVAMENVRDAWVRRVEFRHFAGSAVYLTETASRITVE
                     DCKSLQPVSEIGAERRYSFYTLGGQCLFQRLWAEYGYHDFAVGAGAPGPNAFVQCWSI
                     SPHSFSGTVDALSSGVLFDICAVHANALRFSRPDPANEGYSYTTANSMFWNSTAAIMS
                     CPKPGSAQNWAFGAWAQFSGKGYWHEANSHVSPWSFFYAQLRDRTGKRNPGENKLIAL
                     SQGGTSSREDARRETLAARKPLILLSDWIDTLCLCEPLSLTYDNKDKQLSKVWQQEPY
                     MIKKAEKSPAPAMQLNEGVLIRDGKILTGGRFNPAWWRGSLLPKNQQAPHLTRYALEP
                     EGYRVVDDLDEVCANMQKSGILVMDHNYGLWYDRRRDDHERTSRIDGEVKAPFYELPF
                     ARSGQGRAWDGLSKYDLTRWNAWYWNRLKKYADLADDKGLVLFHQHYFQHNIIEAGAH
                     WADFPWRSANNVNETGFPEPVPYAGNKRVFMAEHFYDVTHPVRRELHRNYIRKCLDNF
                     AENSSVLHFISAEFTGPLHFVEFWLDVVSEWEKETGCNALIALSTTKEVQDAILKDPV
                     RSKVVEAVDIRYWYVDENGKEFAPKGGLNLAPRQFERMEKPAKTSALEVYNMVSSYRR
                     QYPQKAVIYSADSYPEFAWAAFMAGASLCVLPKDLPEDFLKDAASLSPLAAACWLLAK
                     AGQAYIAYPSDDALIEVDLSKETKPFRAQWIDPVSGRPRGKAFQVRAGTVFKTTGKDV
                     LWLRQTN"
     gene            5840..6784
                     /locus_tag="PLI37_00015"
     CDS             5840..6784
                     /locus_tag="PLI37_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOQ57434.1"
                     /translation="MKKFLVPFLTLLLFTACTPKEVYMFSSFNEPATDGLRLLYSEDA
                     YHWEDLGGIFLKPAVGNQQVMRDPSIVRAPDGTFHLVWTSSWRGDLGFGYASSKDLIN
                     WSEQRFLPVMAFDTTTVNVWAPEIHYDETQAQFIIIWASCIPYRFEKGIEEETGNYRM
                     YYVTTKDFQEFSETKLFYDPGFSVIDAVMVPRAKDDVVLVLKDNTRPERNLKVAFGSS
                     MTGPFGPASEPFTEIYTEGPSVVKVAEGWLIYFDAYRKKTYEAVFTKDFIHFENANDQ
                     ISIPEGHKHGTIFKADQKVLKALKKEMEKRREVESNNQ"
     gene            6796..8676
                     /locus_tag="PLI37_00020"
     CDS             6796..8676
                     /locus_tag="PLI37_00020"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOQ57435.1"
                     /translation="MKYSAISTLLLLFVLTVSCSQTASKSGPLADRVHYTGTSMSNVD
                     YHHGQLSPAIGVHNVQIMRANRGKPESSDGYGWTYNHAPMMAYWSNTFFVEYLSDSVS
                     EHIPPCHTLLVRSSDGINWSKPEVIFPKYKIPDGFKKPGSEVVAKDALAVMHQRVGFY
                     VSSHNKLLALAYYGIALFPKDDPNDGMGVGRVVREIKADGTYGPIYFLRYNQSFNEEN
                     SLYPFYKSCPEADFVAACDEILANPLYMMQMVEEADRDDPLIPLQKQYKAFSYYHLPD
                     GRVVGLWKHALTSISADGGKTWAEPVERAGGFVNSNAKIWGQRTSDGKYATVYNPSEY
                     RWPLAVSTSKDGLEYTDLMLVHGEITAMRYGGQYKSYGPQYVRGIQEGNGTPPDGKMW
                     LTYSMNKEDIWVSSVPVPICSKAAKHTREIFDEMPDGRETALWNTYDLQWASTRIEKD
                     EAGRKSLTLRDKDPFDYAKAEKIFPASAQFTLTAEIKAGQNDHGTLHMEVQDAKGQPS
                     IRISFNDDGRMYSKSGARYSSLGSYEAGKSYTIKLNIDTKMRYYTVQINSGREVGRIF
                     YAPAASLERVVFRTGEPRLHPTPETPADRYTDLPAADTADPEACFRIYSLETAHATGN
                     NQ"
     gene            8690..10258
                     /locus_tag="PLI37_00025"
     CDS             8690..10258
                     /locus_tag="PLI37_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57436.1"
                     /translation="MKKQLVASLLIGFTVISFASCQAKIKTPKSANILKAKDFKHYVD
                     YFNRMEDEPIVQAIPNAESWDWMQANIPLFSCPQDNFEEMWYFRWWSLRKGIRETPLG
                     FVINEFLVERSYADQYNMISCALGHHIYETRWLHDSKYLDQYLHVWFRGNEGAPMKRL
                     RSFSSWTAYAVYNRYLADGNKEYMLDLLPDLEKEYRDWEGDHRLPDGMYWQGDVQDGM
                     EESVSGGRRIKNARPTINSYMYGNALALSKMFSLSGETAKAALFAAKADTIKKMVQDN
                     LWHPERKFFETRWVNGQLAQAREAIGYIPWYFNLPDLGKNYEEAWLQVKDPEGFLAPY
                     GLTTAERRHPEFRTRGVGQCEWDGAVWPFASSQTMTAMANVLNNYEQNYVDKATYYEL
                     MNLYVESQYHRGRPYIGEYLDEVTGYWLKGDQLRSRYYNHSTFADLIITGLMGLRPGE
                     DNILVVNPLIPQEQWDWFCLDNLMYHGKNIAVVWDKSGKKFNKGQGLLIFCNGKKVAQ
                     KPTIEKITVDISPY"
     gene            10329..11354
                     /locus_tag="PLI37_00030"
     CDS             10329..11354
                     /locus_tag="PLI37_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ57437.1"
                     /translation="MAFKKKVSLKDIAQAVGVSSALVSMVLNGKAKQYGIGEEATRKV
                     LAKAKELNYQPNIMARGLRSGRSRLLGLVVADISNPFFATLAREIERAASERGYTVIY
                     GSSDEKPEHLSKLMNTLANQGVDGIIVVPCENSEELIADLYLQKYAMVLVDRYFPKIN
                     TLSVSLDNAEACKNVTRHLLSQGYKKIGFVSYESELLHMTERVRGYCEAMQEAGLKNS
                     INVLKLPQDKYQETMLQVFEQHQIKNLEALIFSNNSLTIQGLYVLKQLKVRIPEDLAV
                     FGFDGGDVFDLYCPAISYVRQPVEEMGRKAVTVLIDQLESEKPGEIKRYVLKSDLVIA
                     ESSLKKK"
     gene            11431..>12092
                     /locus_tag="PLI37_00035"
     CDS             11431..>12092
                     /locus_tag="PLI37_00035"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOQ57438.1"
                     /translation="MTKNFSGVVVPMITPLLNDLRVDTAAVKRIMKLFSAHSISPLVL
                     GTTGESSSVSRKESLRCIRAACRAKSGTQKVYAGLVGNEVGELLDRGKMYADMGVDAV
                     VSTLPSYYPLSADQMRSFYLTLADSLSVPVLMYNIKATTQMSLSLDLVAELSQHPNIA
                     GLKDSERDPARLNECIQKYKNRSDFSFFCGWGAQGANSLILGADGIVPSTGNVVPEMY
                     YK"
     assembly_gap    12093..12192
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12193..13392
                     /locus_tag="PLI37_00040"
     CDS             <12193..13392
                     /locus_tag="PLI37_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57439.1"
                     /translation="ARIYSSLAFILTHFSKMGTVLYLVSLALHSMIGVNVIWVIWFLG
                     IAIVLLTLLGGMEGVIWMDVIQGILLICGGLLCAGILLFRPEGGPVSVLTEAITTKKI
                     GFGPYDFNFAELTFWVMAINGIFYALQKYGTDQTIVQRYLTAKTDRDAKKAAYIGVGL
                     SVPIWTLFMLIGTALYFFYHSGTAVLPEGLKADEVFPYFIINELPVGIVGLVIAALVA
                     AAISSLDSDMNCLAAIGVEDYYQRLKPNCTDKQRLNMGRILVAVSGLASIGVASLYAV
                     WEGEGVLGVIFELYAIFSAGIVGIFLLGLFSKRANKQGLYIGMLVCVLFTAYAVLTST
                     SVEVGGSKRLILDLGSMNFPHHKYMLGVYSHLIVLVVGYVASLFFPKPQLEKGLTIFD
                     YKKPGKN"
     gene            13399..14547
                     /locus_tag="PLI37_00045"
     CDS             13399..14547
                     /locus_tag="PLI37_00045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOQ57440.1"
                     /translation="MRSIELIQPKHIIFGAGYAQDWANIVLAGKKQRVLLLSTHPLWA
                     VLEKSIGNAREKGLELLCLDYSWQAEPDTEIFENLYRQSKDFGPQAIVAIGGGSVLDV
                     GKLLAALSSGTQKPSDVFGKNLLDSRDNLLICVPTTAGTGSEVSPNAILFDLRDGEKK
                     GIISPWLIPDTALIDPEMTFSLPPKLTAETGMDALCHCIEAYTNKYAHPAVDLYALKG
                     IEYVYKNLKTAVKEGNNIEARSAMALASMYGGLCLGPVNTSAVHALSYGLAGKYHFSH
                     GLANAVLLPAVMLFNMEADLEKNARIALAMGLPDKGDLRATARSGIKAIQELSRDCGI
                     PQSLSSLGVKREDLPQLADMAMNVKRLLNNNPRELEWKDALSIYQSIL"
     gene            14553..>15160
                     /locus_tag="PLI37_00050"
     CDS             14553..>15160
                     /locus_tag="PLI37_00050"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOQ57441.1"
                     /translation="MNKTNNTKPVIGISMGDPAGIGPEIIVKALSSAEVYAACRPVVV
                     GDAGVMRQAVQLLGKDLKIHPVQSIPEARFEYGTIDVYDLHNVDLSKLQHGVVSAMAG
                     GAAFEAVRCLIDLAMTSQVDATVTAPINKESIHKAGHAFSGHTEIYAHYTNTKNYAML
                     LADDALRVIHVSTHVSLRQACDRVTKSRVLDVIELLNKACVQ"
     assembly_gap    15161..15260
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15261..15464
                     /locus_tag="PLI37_00055"
     CDS             <15261..15464
                     /locus_tag="PLI37_00055"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOQ57442.1"
                     /translation="LLGFKWNNETQKMDSVKGVNITLGLPIIRTSVDHGTAFEIAGKG
                     IACEDAMLISIDYALKMAKNRIQ"
     gene            15461..16654
                     /locus_tag="PLI37_00060"
     CDS             15461..16654
                     /locus_tag="PLI37_00060"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HOQ57443.1"
                     /translation="MIVIIADDLTGAAETVGICLRHGVGAAMIMEIPGEERLQEYTKE
                     GIAVLVIACDTRSGTLEEACRVNRLLGETLKQAGFTQIFKKIDSVLRGWVVPELNTLA
                     RLTGKSSLLIQPANPGAGRCIRDGKYFINGIELSETAFGLDPEFPASSSDVMEILQER
                     LPGKEPASVIKFSIPDACYPEDLRNSALQCGPDVLPAGSAAFWEAYLEEQIKRGLILT
                     AGINYRPHTQRKKPPVPSTHNFLMICGSAHQHSLDYDEYLRKQGVTLMEMPPELSSEQ
                     EPDPADPAAWASACVEAWKKEPRLALRIASKPINYENSAHKLKARFTDALQLILQQIK
                     PAELFIEGGATAASLLNRLNWTTFKPVDELAPGVVRLQLMPEPSLFITLKPGSYFWPE
                     FLFNS"
     gene            16658..17899
                     /locus_tag="PLI37_00065"
                     /pseudo
     CDS             16658..17899
                     /locus_tag="PLI37_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
     gene            18312..>19333
                     /locus_tag="PLI37_00070"
     CDS             18312..>19333
                     /locus_tag="PLI37_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57444.1"
                     /translation="MNKIWLTVLLCISVCAYSQQNSDNCFRRPLKEVLTDLQSQFGVR
                     LRFSEELVRDKVLDYADWRIHPWSLEESLNNVLLPFDYKFVKESSTSYEIKAFEYARR
                     TADFGRDFLDYLSSRHADLPAWEARKALLKKEMKIAAGLDPLPRSPGTKPILGPKRKY
                     EGYYVQNIALEILPGVFCTGSIYHPIGNKGKCPIMLNPNGHFGDGRYRADQQLRCAMQ
                     ARMGIIAVSYDLFAWDEQLLQFDSRSHRSSMAHTIQSLNAIRMLDYLCALKEADLSRV
                     GITGGSGGGSQTMFITAIDERITLSMPVVMTSSYFVGGCPCESGNPIHLCGGRTNNAE
                     ITAMCAP"
     assembly_gap    19334..19433
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19635..21305
                     /locus_tag="PLI37_00075"
     CDS             19635..21305
                     /locus_tag="PLI37_00075"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOQ57445.1"
                     /translation="MVKYRIFALCLIVTGLFYACQSPEIDLQAEHLRLDYNGGEGARQ
                     LCLSWTMNASSPRGLNQQAYRIRIASRPELLEQNSPDIWDSGKVSSSKNYDVPYKGKA
                     LERDTVYYAQIRIWTNRGKSLWSKQQNLVLPAEGDLLPAAASINRFDTYDLKLSDLVQ
                     TYKTATLEKYKNRPQDLENMSAADCWAESYLIDNRKFYLQWMDRLLAAQAEDGSLPLS
                     AQQEPQEIFDIAHMLLVQYNETAHLKKHFPAMEKWLDFLWKNYGSQEYILTANGDGHT
                     LLPVEEYSLDGKEYITSAALISTAYFYRLCVLMQKFSSHLQYRDKAAHYADWAGKLRK
                     AYNDRFFNEEKQYYENNTPTANLLSLSLGFCEDKRAESVFKRIEQSIAIEHYGKAACG
                     EFGKRYLYRTLSAWGRSDLSYRIGAELREVEGDFILWFFEHLCGLKSLEEGFHCFSLD
                     AVFPPGIYFAKTRMETRGGIISSSWIKTFDEFVWQVIVPVNTRARLRYPAAAVKDIYD
                     DGRVVDQVEGIQILGRENGNILMELGSGDYFFSSKLADIKPGPRRNNE"
     gene            21335..23242
                     /locus_tag="PLI37_00080"
     CDS             21335..23242
                     /locus_tag="PLI37_00080"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOQ57446.1"
                     /translation="MKTKSMLLTMGLLLSFTACRQGAEWKKGILIDEFVYEKTSFPEC
                     HSASLAETPEGIVLTFFGGTKERHPDASIWICRNENGVWTEPEKAADGVVNDTLRYPC
                     WNPVLYQVPGEELLLFYKVGPSPGSWWGCMKRSFDNGKTWSAEEKLPEGIIGPVKNKP
                     VVVDGKLFCPSSTENRGWRLHIDVTEDKGKTWTQIGPLNDPAEWLAIQPSILFHKNGD
                     LQILCRTRNRLVGSTWSTDRGRTWSQLEGVGLPNNNSGTDAVTLKDGRHLLVYNHVEP
                     PAGETKGARTPLNVALSKDGKTWYASLVLEDSPISQYSYPCVIQSSDGFVHIAYTWRR
                     QRIKYVKIDPAKLKMKEIENGIWPGKETAQAVEGAGSTPFPISVCDWMILKRQKLGAI
                     TLAREIGCDGLEIDMGGLGNRPTFDNKLKDPEVLAEFKRTAQVSGIRFSSVAMSGFYA
                     QSLVDREDFITPVQDCIDVMKGLGVGVAFLPLGVKGDLVQYPELRPAIVERLRVIGRM
                     AEQEKVVIGIETSLDAEGDKALLQEIGSPGIKIYFNFARALQSGKDLHRELRLLGDDI
                     CMIHCTNKDSVWLENDPMIDMPAVKQTLEEIGYRGWLVMERSRDASQARNVRGNYGAN
                     CAYLKSVFQPE"
     gene            23376..25244
                     /locus_tag="PLI37_00085"
     CDS             23376..25244
                     /locus_tag="PLI37_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOQ57447.1"
                     /translation="MIKKSFIVLALLCSSLSIFARTEIWVAPEGSDQAPGTKAEPKAS
                     LHSALRQARESRRLSDPSTADGIIIRMQAGTYFLDEPLLIRPEDCGTPESPTRIVAEK
                     SGEVTLSGGVKLGKWSKAGSVPGLPSAAKGKVWVTDQPKVSGRYIDFRQLWVDGRKAV
                     RARDTNDDNFKRILAWDKNTGVLTIPAPGVKSFKTLDRLELQLHQMWAIANLRIKAIE
                     YQNEKALVVFHQPESRIQAEHPWPTPMIAEGRESPFFLCNAIEFLDQPGEWYLDVEDH
                     KLYYWPRPGEDITRTESVVPYLETLIEVQGSLDRPVKHISFEGIGFSYTSWLRPSEQG
                     HVPLQAGMYLLDAYKLRPPGVPGNENRGLENQGWVGRPPAAVQLRGVQNTSFVRCRFE
                     HLASCGIDYISGTLNDSILACVFQDIGGNGIQAGKFSDPGIETHLPYDPVDRREICTK
                     LYIGNNYIHNCSNEDWGCVGICAGYVRDILIEHNELSELSYSGISLGWGWSKTVNCMR
                     NNVVRANYIHHYAKRMYDVAGIYTLSVQSKSLISENVVDSIYSPAYVHDPNHWFYLYT
                     DEGSSFITVKDNWCPAEKFLQNANGPGNTWINNGPMVDEKIRLAAGIEPAYSDIKP"
     gene            25796..28984
                     /locus_tag="PLI37_00090"
     CDS             25796..28984
                     /locus_tag="PLI37_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ57448.1"
                     /translation="MRKLTFLLACLFLASVGLVNAQSRTVTGRVIYAEDEQPVIGAYV
                     VVQGSSRGTVTDADGNFSISVPQEATILEFSFLGMKTVQAEARNGMVVRMEQEVSELD
                     EVMVVAFGTATKKSFTGSAAVIKAEDITTRQVSNVTNALAGQVAGVQTVNTSGGQPGN
                     AATIRIRGIGSMSASNAPLYIVDGAPFDGSMSSINPQDIESISVLKDAAANAIYGARG
                     ANGVVLITTKRGTTQEAKVNVDAKWGNNSRAVPNYAVMEDPAMYYETFYKALYNSKIY
                     NGNSANDAHVYARSVLFDKDAGGLGYQVYTLPAGQSLIGTNFKLNPNATLGYKDADFY
                     YTPDNWYNEIFDPNNLRQEYNVSVSGASDKINYFMSLGYLDDSGIVSGSGFSRYTART
                     NVEYQAKEWLKVGANIGYTNYDIQSPGSQTNWGSSGNLFYVTSLIAPIYPLYVRNSTD
                     EQIRVDHRGITVYDFGTTTNQKRAFMGMSNPAITLKLDEHHAYTDVLASRFYGTLDLF
                     KGMKFTASVSANTINQRANRLYNQFYGAAVASQGAVSVSHARGVGINQQYMLNYKRSF
                     GVNGLDILAGYESYELTEQYLYGYNTKLFNPYIGELGNAIDVDSRDMTSYTNHYSTLG
                     FIGRAEYDYDQKYILSASYRRDASSRFAPEQRWGDFGSVGAAWAINNEGFMANAESIN
                     LLKLKASYGIQGNDNIGNYYAYLDQYTVSNADGDFSVGFAYKGNPDITWETSYSFNTG
                     IDFSMFDERLNGTIEYFSRKTVDLLYNQPVPLSFGYASIPTNVGAILNNGVEIDLRYT
                     LVKTNNLTWDINVNATHYKNTILDLAESVKEAGGIKQSTYIYRVGGSLYNTYLREYAG
                     VDPVTGKALYYMDPDNDDYSTTDVWSSAKQADNGSSLAKVYGGFGTALQLRGIDLSAN
                     FSYQLGGKLYDFSYEELMHSGDNAGLNWHTDILNAWTPENTVTDVPRICSSDDTYQTY
                     STRFMISSNYLSLNNITLGYTLPKRWTEKIKIEKLRIYAVGDNLALFSARKGFDPRTM
                     IGAGVGSLGTAGTGSHTYTALRTISGGISLTF"
     gene            29012..30652
                     /locus_tag="PLI37_00095"
     CDS             29012..30652
                     /locus_tag="PLI37_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ57449.1"
                     /translation="MKKRNIFFLALGLVLLGSCTDLNTIPEGGTLTEEQKREVVEAIP
                     GRLAADINGMFAVLSKQYCVFGAASGRDDDHGYPAAAMSWDLNGPDMVGTDDNYNWFS
                     VASDYADRTYTYANPYMRWAMFYKQIKMANDVMASIPEDTDNPTLLTYRGLAHAVRAF
                     DYFSLVQYYQFTYGPETLDKPAVPIVEPGMTDVTNPRATVRQVYELIMNDIDTAIVLL
                     KDYVPTSKSYISEAVAYGLRARVNLVMKNYAQAALDARKAIDLFGGTPYTREELMTPG
                     FIDATDHNWMWALIITPDVVPDAYPAWPAVLASFSGDSYTCGVGCYKMINTLLYSIIP
                     ATDVRKGWWVDAELKSPLIDSLSWPEYKGEPLGPLNIPDVKMPYLPYTNVKFGQFGGI
                     GNTVNAGDWCMMRVEEMLLIEAEATGMTDEAAGKNLLTNFVKTYRDPAYTFPASGIKF
                     QDECWKQRRIELWGEGFSMMDIMRLKKNIVRFNSRVETNHPEAFRFNLAADDGWLLMR
                     IPQREINSNPGIGDEDNNNDGALPVSGDGAGLRDGVTD"
     gene            30683..31594
                     /locus_tag="PLI37_00100"
     CDS             30683..31594
                     /locus_tag="PLI37_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57450.1"
                     /translation="MKNFKRIFFIIGLGAFLWSSCTEDIPVREESPAADPESMQVYFP
                     NSQKYKYELPPTVFKVDIDLKREKSAKAATVKIIVTSDLTGVFTVPETVSFAAGDSTA
                     KLTITFDTLEQFKPYQISFKIEDQYINPYVVNPDGTTDFNLYVEHNDFLPVGNGKGQF
                     FDSFTLASVADVSITYSEKKDVYRISNPYTLALLEEAEWGNWIGGPISENIEIQITSE
                     GKVTWEFWYLGLNYQGVSGYPIKAYFPSVLDESLAALDDKSVKLQDKLFKLHPYFYID
                     GLGGFGADYPVFISFPGGPNLNELLAQ"
     assembly_gap    31695..31794
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            31990..35106
                     /locus_tag="PLI37_00105"
     CDS             31990..35106
                     /locus_tag="PLI37_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOQ57451.1"
                     /translation="MYRKQILIFIFCLLCSRALWAVMAYPYPVEVDLPDGSKLEIQRH
                     GDEFFNYTTDQNGYIIAQKPDGYYYYANYDEQAKLNISNYRAGSRAARRSAVVPEVNF
                     RRAAQTRSERYQASRDLIRANAKQAYEGRLKTLVLLVAFQDKAFVSPTAHDDFYRLSN
                     ELGYSDNGATGSVRDYYRDNSNGVFDPEFVVMGPYTLSKPMSYYGGNNEEGNDNDPRR
                     MVIEAAWMANEEGVDFSEFDQNNDGYVDFIYVYYAGYNEAEGGPAASIWPHSWSVAYM
                     DSTLLDGVKLGYYACSSELAGASGSTMSAIGTVCHEYGHTLGLMDVYDTDGAESGGMS
                     GGLGALSLMSSGNYNNKSRTPPHLTSEERWSLGWMTPTTITEAGDYSLASIDQNQAYI
                     IPTLNENEYFLLENRQHNNWDLYIKGEGLLIYHVDKSRNMVLGKTALERWYTNTVNVY
                     PQHQCMDMEEAVGSESKGDMYAFYPGPSGLYTAFTRTTTPAALAWSGLPLGKELYDIR
                     LEGQEIHFRVELEDQVYFGGQVINREGQPVQGALLYLKELVEQPVSGNTTGLRSAMHI
                     SGVYYTTTSGIDGRFIFDEDLDPGVYELLCQSEAYISNSQIVELKPGSNSVLITLLTE
                     AEQLIEGTLSWCRDFSYGGAIGNEGESFVAGASWTAEELSQYRGMYIGRMTFFIAGGN
                     PDVEVSIYADRNPIFKKNIAAEDLTANSFYSLNLMADSLVINPETELILAYQVSNYPK
                     NAYPAGICGGSRVEGKGNLISLDQGATWTTLYQEAEIEGNWLISFDIYRGSDFVAMES
                     FNLDPTDLTMKIGETRYLYGQIKPANATNGKISWSSSDSSVVWVDQTGKVFALAAGQS
                     VVTATTDGGNRSATCQVKVVPTIASSLNVNVAQHVFFVDWDEATGDAWTFSWRNLGDS
                     VYQDTTLTQSSFLLEGLDPKSTTEVLVQAYSEQQLVDEHAFSVTTANKKSDFMAIYTT
                     ETFSPGQKLLLDVLNLPEDASVNVWKLNDEVVSGSILELSEGIHELRVEITTSKGIEI
                     IRRQLKVE"
     gene            35108..35533
                     /locus_tag="PLI37_00110"
     CDS             35108..35533
                     /locus_tag="PLI37_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57452.1"
                     /translation="MRRHFSILALILLTGLLYLACVREDPLLKAAKEHLVSQEIYGMY
                     KDRQAVFIYDADKHQLADNAEALYFRIQNDSQNTILQMTLDQEPDLGKKCLMTIDAKG
                     LSGIETSYSVEVVKMDESKQTLWLFDQTGKTGFVMFYRF"
     gene            35541..37028
                     /gene="dacB"
                     /locus_tag="PLI37_00115"
     CDS             35541..37028
                     /gene="dacB"
                     /locus_tag="PLI37_00115"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOQ57453.1"
                     /translation="MRIFRVFCLLCICSFGFGAEKSIQLRADEYPGTSLAYVVQEVQS
                     GKLLAEMNSAQALVPASLSKLLTTGAALEILGPEKRFKTELAYRGLLEKGSLKGNLYI
                     LGGGDPSLGSEYTEEQAGLFLQDWAQAVKELGISRIEGDIVADPSIFDQEAVSPYWLW
                     EDMGNYYAAGVYGLAVFDNMFRLGLKSGLPGTKPEILYLKPALPEMRIINNLIAKDNA
                     KDSAYFYGVPYRWERVLYGSLPAGREEFVIKGDIPDPPAYLAFRLQQELIKAGIDVQG
                     RALSKQEPGSICKEPGFFPKEYVNSANNPQADHEAVVFYTGYSKPLGELVKIINYRSH
                     NVYTEYLLRHLALAAGAKAPVSAREGLEALQRFWSGKGVDISAWQLYDASGLSPLNRL
                     DAASLAQVLRYMALESPRAGLFSGSLPQAALEGTVADFGKTLPGTLRMKSGSMKSVTN
                     YAGYWRLGDKTYVVVLLCNQHKQSAARIRRDMEQLLRTIYNKGLK"
     gene            37025..37804
                     /locus_tag="PLI37_00120"
     CDS             37025..37804
                     /locus_tag="PLI37_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57454.1"
                     /translation="MNKEELFKELKELNLLWSGLSRIPNFPKELLELSLNKLENINRL
                     VKDLEETSYQPQVKPAFEKETHTREATPAPLAADLKSKPSVAEETKPIPAPQSSTPTP
                     ASSSPAQSSPLTTSVSPLSTRERSLPTQESSLPVKESGQTEEPVSPPVGKMPKLQTEK
                     PESLVDRYEKTSSKESLQKKLESRHFADLSKALSLNDRFRFRRDLFGDSPETMEQVLQ
                     DINTMQSMDEALSYLHKNYSQHSETESFGDFCKLLNNHFSS"
     gene            37819..38535
                     /gene="rsmI"
                     /locus_tag="PLI37_00125"
     CDS             37819..38535
                     /gene="rsmI"
                     /locus_tag="PLI37_00125"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOQ57455.1"
                     /translation="MSKLSIVPTPLGNLEDITLRALRILKEADLILAEDTRTTSKLLK
                     HYDIGTPMQSHHKFNEHKTVEQVVARIQAGEHIALVSDAGTPGISDPGFLLVRQCLQA
                     GLQVECLPGATAFVPALVVSGLPEERFHFEGFLPQKKGRLTRLRYLAELTHTFVLYES
                     PYRVLKTLTQLAEICGPERRASVSREISKIYEETLRGSLDELIRHFSLTEARGEFVLV
                     VAGKNESREQSSLEKDHPRE"
     gene            complement(38554..41478)
                     /locus_tag="PLI37_00130"
     CDS             complement(38554..41478)
                     /locus_tag="PLI37_00130"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOQ57456.1"
                     /translation="MKKSNLRSFLRFFTAGFFLTFAVTAVAQNGPVGSVSHFKLGNGM
                     QVYIWPDSNQTDVHGRVSVHVGSKNDPEQFTGLAHYLEHLMFKGTDRIGTTDWEQEKI
                     LYEQIIAKYDEKAASSDPKTKEALNKEINELSIKTSEFSQSNEFSSLVATMGGTGMNA
                     GTSYDLTTYYNDFPPNQLERFLMLYAERFRAPVFRTFQSELETVYEEYNLYEDQLEQR
                     EFHYLLNNLFKGHPYERPIIGLSEHLKNPQISKLIEFYNNWYVPENMALILVGNIDPQ
                     AARPLIEKSFGALPAKTLPSLPEFSDWKVEGRKDFKANLAYIPRIHVGFKGAGITNPD
                     ALVIDLFTNLLSNRSRTGYLDKLVLDGDLMGASASHISFEDQGRIVISAIPSYDPAQQ
                     VFASTKAVEKLLFQEIERIKQGNIDAKLLENIKKAMIRSYQLNLESNSDKAEILTRAF
                     ISGKALDEALDYTGRLNKIQITDIQRVASDYCNNNYMLFDIGTGKFPAAEKLEKPDIK
                     PITQSSEKKSAYALAFEDLSYEKQSLPVKDFEEIIIKPINTRSKLFYTQNTENEVFSL
                     ILKYGIGTEKMPYLEYSTTLMNYAGIMGGYDPQEFKKAITELNVTCRYSVDEDYLYVV
                     MEGFEEHLVEALGLLTRQILMPQLDEKQYRNVQGSIYQSRIREGKDPQIIAEALAEYM
                     VYGDKSAYLDRPTLYNIFTEMGLSKLTGEFQRATDYEAEVHFCGSMPFDEAYDILSQY
                     LPLKANEKESSSPEWKEYKEYSENTIYFLPRSDTKQAKIYFFAPGNVYKPENDLKYKA
                     FNRYFGAGGLTSLIIEEIREKNSMAYTAYGFLATPPLAEKQVCFQGYVGTQSDKVNDA
                     VALYMKLVDDMPLYGEREKNLKAYLELEPFTTQPDFRSKSQQYEEWKRLGYTANPALQ
                     QEAEIQQFNLQQMLEFYQQELKGKPMAIAIVGDPKLIDLKALGAYGKVVKISSTKLFS
                     KD"
     gene            41617..42852
                     /gene="hflX"
                     /locus_tag="PLI37_00135"
     CDS             41617..42852
                     /gene="hflX"
                     /locus_tag="PLI37_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOQ57457.1"
                     /translation="MQPNFTFDTFSERSVLVGLITPEQNEQKATEYLDELAFLAQTAG
                     ATPVKRFLQRLDQPNPKTLVGSGKLLEIKEYVENNEITMVIFDDDLSAKQLKNLDQVL
                     KVKILDRTSLILTIFAERAQTAYAKTQVELAQYQYFLPRLTRLWTHLERQRGGGGVKL
                     RGPGETQIETDRRIILNKIAQLKKELKQIDKQKVVQRSNRGKMVRVALVGYTNVGKST
                     LMNLLSKTELLVENKLFATLDTTVRKVIIDNLPFLLSDTVGFIRKLPHDLVESFKSTL
                     DEVREADLLIHVVDISHPGFEEQILVVENTLTELGVHNKPVILVFNKIDAYSFVPKEE
                     SDLSPELPENRTLEALQASWMSKMQDNCLFVSAVERTNIDNLKQLLYDKVKEIHVTRF
                     PYNDFLYPLYDVSEKDSSL"
     gene            42865..>43484
                     /locus_tag="PLI37_00140"
     CDS             42865..>43484
                     /locus_tag="PLI37_00140"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOQ57458.1"
                     /translation="MIPPYRKLTPSEIEQLGSQACTADDWDKVLVHEDFSAEAIRSVH
                     FSGDIKLGKFEACHDLPGNVKKKSGIRRAHLHNCEVGNNVLIENIGEYIANYVIEDNC
                     CIEQLTFMICDAENSFGNGVEIEVLDETGGRVSRMYNGISAHTAYLMAMYPGRNDLQA
                     QLKKLTEHKAMQERSAMGRVGHSSYLINAGMILNVNMGPFTKVRGSS"
     assembly_gap    43485..43584
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <43585..45083
                     /locus_tag="PLI37_00145"
     CDS             <43585..45083
                     /locus_tag="PLI37_00145"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOQ57459.1"
                     /translation="GMILNVNMGPFTKVRGSSFLYNGSINSTEEAPVQVGAEVNAQSF
                     IFSPGSEIKDGVHLVRCFVGEASILGHGFTGHDSFFFANCRMENGEACASFAGPFSTS
                     MHKSTLMIGGLFSFCNFGSGSNQSNHLYKLGPMHSGVLERGTKTGSDSYLLWPAKIGP
                     FSLVKGRHVNNPDTTAFPFSYLVENRGMSYLIPAASLPSIGLLRDVNKWPDRDLRKSS
                     KKQDKIIFHFLNPNTVEEIRKGIRIGNDLRLRASLGYNPEDAAMQGVVSELRHTAPDD
                     PILEYRYRGMRIRRSNLEKGSALYKMAIQIYVGDVVLRQIEAFKQKKDREDDKIIWKD
                     FIKTRVFEKEIPYNQEKWLDIAGLVAPEAAVEYLIGCIERGKFTSVEQLEDRWEELYQ
                     TYQEREWAYAFPLIAELRNIALRDIKPATLLAILDEWQEASLTLCNQALLDAELELSM
                     KRSFAPEEGEVPNSFVEKLRKSIGEIPGKGDVARQFIQDNIVMQNNLH"
     gene            45116..46753
                     /locus_tag="PLI37_00150"
     CDS             45116..46753
                     /locus_tag="PLI37_00150"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOQ57460.1"
                     /translation="MSTPEFKYQNPFPLGKDDTEYYLLSKDYISTAQFEGREILKVQP
                     EALRLLARTAMRDCSFLLRPAHQAQVAAILDDPQASENDKYVALTMLRNAEVSAKGVL
                     PFCQDTGTATVVAKKGQEVWTGGGDEEALSHGVYDTYTSENLRYSQTIPLDMYREKNS
                     GNNLPAQIDIQSVDGNEYHFLFIAKGGGSQNKTYLFQETKALLNPKTLEAFLIDKMRN
                     LGTAACPPYHIAFVIGGSSADMTLKTVKLASTHYYDSLPLEGNDYGQAFRDVKLEEKM
                     LKAAGELGLGAQFGGKYFAHDIRIIRLPRHGASCPVGLAVSCSADRNIKAKINKEGIW
                     LEKLEENPGKYIPEALRRSGEGDAVKIDLNRPMKEILADLSRYPVATRLSLNGTIIVG
                     RDMAHAKLKERLDEGKGLPQYIKDHPIYYAGPAKQPQGLPSGSFGPTTAGRMDSYVEL
                     FQRNGGSMVMIAKGNRSRQVTEACQKYGGFYLGSIGGPAAILAEQNIKSVECLEYPEL
                     GMEAIWKIEVENFPAFILVDDKGNDFFEQIKPACLNC"
     gene            complement(46757..47686)
                     /gene="rsgA"
                     /locus_tag="PLI37_00155"
     CDS             complement(46757..47686)
                     /gene="rsgA"
                     /locus_tag="PLI37_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOQ57461.1"
                     /translation="MEGLVIKTAGNVFLVKTLAGDLHECVVKGNFRIKDLSSTNPVAI
                     GDRVLIEKTSDEQTYFITEVLDRSNFIVRKAANLSKQLHILAANIDLAILMVTVNHPQ
                     THTIFVDRFLATAEAYRIPCCLLFNKIDLYGAEDSEYLEALIRLYESIPYPCLKVSAL
                     KGEGIEEVRSLIRGKICLLAGNSGVGKSSLVNRLNPAIQTKVGPISDYHNRGRHVTSY
                     SEMFEIEEDTYIIDTPGIKGFHTVDMHVAEVGHFFPEIFRTSARCYYNDCTHRHEPDC
                     AVLKAIEEHRISQSRYLSYLSIIEDAGDGKYRE"
     gene            complement(47694..48227)
                     /gene="frr"
                     /locus_tag="PLI37_00160"
     CDS             complement(47694..48227)
                     /gene="frr"
                     /locus_tag="PLI37_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOQ57462.1"
                     /translation="MTEENMSQTIDFLTKTLSRIRAGKANVSILDNIRVDYYGTQTDL
                     DKVANLSTPDPKTIAIQPWEKNMIKEIERAIMASDVGITPVNNGEIIRLAVPPLTEER
                     RRQLSRQVKNEGEEAKISIRNARRDTIESIKKLVKEGLSEDIGKNAEAEVQKTHDKFI
                     KSIDEIVALKEKEIMTV"
     gene            complement(48286..48993)
                     /gene="pyrH"
                     /locus_tag="PLI37_00165"
     CDS             complement(48286..48993)
                     /gene="pyrH"
                     /locus_tag="PLI37_00165"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOQ57463.1"
                     /translation="MKYKRILLKLSGESLMGKSNYGIDPGRLNEYAEQIKELQIMGIQ
                     VGIVIGGGNIFRGLSGSQSGFDRVKGDQMGMLATVINSLALSSALNKLEVSNHVLTAV
                     NMEPVAEHYSTDKAIDFLEKGHVVIFSAGTGCPFFTTDTAASLRAVEIKADLMLKGTR
                     VDGVYTADPEKDPKAVKYDKISFEEIYDKNLKIMDLTATTLCRENNLPVVVFNMDKKG
                     NLVKVLKGENIGTLVHN"
     gene            complement(49036..>49361)
                     /locus_tag="PLI37_00170"
     CDS             complement(49036..>49361)
                     /locus_tag="PLI37_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOQ57464.1"
                     /translation="QFSPNLPNPVSVFQLCGRTFDLVDLTLALHCRLGEAYAKTFTEE
                     GKTEIAAAYHRHLYRKEGFHHYKQGNRPFRARILRVMPSGQLEVESDEGWIRAFAFKE
                     IEYLI"
BASE COUNT        12941 a        11194 c        11997 g        12629 t
ORIGIN      
        1 tattcttatt gcggctacat ggcttccggg caacttattc ccgaagtgcc cgttaaggcc
       61 gtggttgctt ggatgccggg agataatacg gcgagaattc agcaggccat cgattatgtt
      121 tcctcgctgg agcccgatgc tcagggtttc agaggaactg ttttgctgga aaagggatgt
      181 tatgaaatcg ccggggggct gaccatatcg gcttcggggg ttgttttacg tggcagtgga
      241 tatatggacg gccaaacgga acttttgggt accgggacgg accggactac catgattcgt
      301 gttgccggga aaaaagatta tcaaacaggc gaaaacaagg agataagctc ttatgtcccc
      361 accggagcaa gggccgttgt ggttcaaggt cattccttca agcccggaga tcgtatacgt
      421 attgcgcgcc cgggtactca ggcatggata gacgctatgg acatgaacga ttttggcgga
      481 gagtcttcct acataggttg gaaacccgaa gatatggtcc tctattggga acggcaggtt
      541 aaagaagtgc ggggcgacag tattctgctc gatgctcctt tgagttgcgc tttggatccc
      601 gaatttggcg gaggttttgt gagcagtttt gaatggcccg gacgtataag ccgctcgggc
      661 gtggagaacc tcagattgcg ttcgacgtac gatgccgata atcccaaaga cgaaaatcat
      721 cgttgggtgg caatcaattt agataacatc gaagatgcct gggtcagacg gatagaattc
      781 aggcattttg ccggttcggc cgttttcctt accgaaaata cacgcagaat aaccgtgaaa
      841 gattgtaaat ccatagaacc gatttccgaa ataggcggac agcgtcgcca aacttttttt
      901 acactcggag gccaatgttt gtttcaacgc ctctattccg agtatgggtt tcacgatttt
      961 gccgtcggac gttgtgcgcc gggccccaac gcctttgtgc aatgttatgc ctggatgcct
     1021 tataatttca gcggtacggt agattcctgg tctaccggag tattgtttga tgtagtaagc
     1081 atagagggac acggcattta tttttccaac agaggccagg atggtctggg agcgggttgg
     1141 actacgggta attcaatgat gtggaattgt tccgcttcca tgttggcctg ccctctgccc
     1201 ccgacagcct ataactggtc ttacggagcc tgggggcaac gtagcggtaa tgccgcctgg
     1261 aacagcgccg gttcttttgt gaaaccccat agtctttact acgcccaact ggcagcacgt
     1321 acggggaaaa aacatcccga cgaagataaa ttaatgcctt tcgacaccag ctccgctacc
     1381 aatccgcccc tagacagagc gcaggagttt gtcgccgaag cacgtaagcc ggcattggaa
     1441 ctgaaagact ggatggatag cctaacatta agtaatccac ttactcttac atacgaaaag
     1501 tccaaaaaga atgatccttc cgtggtctgg cagaaaaaat cggcaggtaa aacaagtacc
     1561 gtatcgcccg atcccgccgc ttggatgaag ctcgaaggcg gtgtgctggt aaaagaggga
     1621 aaaattctta caggaagccg ccggggcgta gtttggtgga gcggaaattt gagacggagg
     1681 tatcttgcta ctgcacgtcc ccacattacg cgttatgctc cggggcgtac gggtacgggt
     1741 ttcaccgacg atctggacga aatgaccgat aatatgctca ggcaaaacgt cctggtaacc
     1801 gatcataact acggattatg gtacgaccgc cgcagggacg atcatgaaag aattatgcgc
     1861 atggacggct acgtttggcc gcctttctat gaattgcctt tttcccgcag cggacaagga
     1921 acagcctggg acgggctgag taaatacgat ttgacccaat ggaatgtatg gtattggaac
     1981 cgcttgaaaa aatacgcaga tttggccgac gaaaagggcc tggtgttgtt ccatcagcat
     2041 tatttccagc ataatatttt ggaggccggc gctcattggg ccgatttccc ctggcgttcg
     2101 gccaacaacg taaacgaaac aggtttccct gagccggtgc cttacgccgg agacaaacga
     2161 atttttatgg cggaattgtt ttacgatgta cagcatccgg tacgcaggga actgcaccgg
     2221 aattacatcc gaaaatgcct ggataatttt gctgccaacg gctccgtgct tcaatttatc
     2281 agtgaggaat tcaccggtcc tctgcacttt gtggaattct ggctggatgt aattgccgaa
     2341 tgggaagcag aaacccggca ggatgccaaa gtcgtattgt gtgttaccaa agacgtgcaa
     2401 gacaagattc tcgatgaccc caaaagggca gccatagtcg atgttataga tattaagtac
     2461 tggagacctt tgccgggagg aggctacaca acgctcctga gtgatttgaa tctggctccg
     2521 cgtcaacaaa cccgggcggg ttctaccagg atacccggag aaggagcggc agacatgccg
     2581 ggcagagtct atgagaccat tttgtcgctt agagagactt ttcccgacaa ggctgtgctt
     2641 tattcagcgg gaggttcgcc ccaggaagaa tgggcagcct tcatggccgg agcctctttg
     2701 tgtaatttgc ctgcccgctt accggaacag ttgctcnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnngcgg gtacatggcc tcggaagcgg agattccatc ggttccggtc
     2881 aaagcgctgg tgccggctat ggaaggcgat gccactgagc acattcaggc agctttgaat
     2941 tacgtctctt cgctgaagcc cgaccaaaac ggttttagag gagccgtact attggaaccc
     3001 ggccgttacg aagtcggtac tacactttgg atcaatgctt ccggtgtggt tttaagggga
     3061 agcggatacg ggccgggggg gaccgaaata atagcaaact acagggacag gacgactctg
     3121 atccgtttca aaggagtgga caacagaagc accggaacct gttataccat tgccgatgaa
     3181 tcgctgcccg taggaacgat gcagatggtt ttgcccggac atcgccttaa agccggagat
     3241 catatccgta tcagtttgcc cgctcgcagc tctcaacagg cagcctccgg acaaaatgcc
     3301 tccggacaag aagcagcccc tgcccgtatg aggaatacgc cctcaatgca atgggaaaga
     3361 gaaatcactt ccgtcgacgg cgataaagta tgcctcgatg ctcccctgac ctacgaaatt
     3421 tcgacacgga acggatcggc cgatgtttgt ttgttgggtt ggccgggccg aattcggcag
     3481 gttggcctcg aaaacctgcg tttaacttcg gtttatgacc aaagcaaccc caaagatgaa
     3541 aatcacgcct ggatagctgt agccatggaa aacgtccggg atgcctgggt acgaagagtg
     3601 gaatttaggc atttcgcggg ctcggcggtc tatcttaccg aaacggcttc ccgcatcacc
     3661 gtagaagatt gtaaatccct gcagcctgtg tccgaaatcg gggcagaaag gcgttacagc
     3721 ttttatacct tgggtggaca atgtctgttt cagcgcctgt gggccgaata tggataccat
     3781 gattttgccg ttggagccgg agctcccgga cccaatgctt ttgtacaatg ctggtcgatc
     3841 tcgccgcata gttttagtgg tactgtcgat gctttgtcga gcggggtttt gtttgatatt
     3901 tgcgctgtgc atgccaatgc cttgcgcttt tcgaggcccg atccggccaa cgaaggttac
     3961 agctacacca ccgccaactc catgttttgg aattcaacgg cagccattat gtcctgcccc
     4021 aaaccaggct cagcacaaaa ttgggctttt ggggcctggg ctcagttctc cggcaaaggc
     4081 tactggcacg aggcgaacag ccatgtttct ccctggagtt tcttttatgc tcaacttcgc
     4141 gacagaaccg gcaaaagaaa tcccggtgaa aataagttga ttgctctatc gcagggagga
     4201 acaagctcca gagaagatgc ccggcgtgaa acactggcag cccgtaagcc cctgatattg
     4261 ctttccgact ggatcgacac cttgtgtctg tgtgagcccc tcagtctcac ttacgataac
     4321 aaagacaagc aattgagcaa agtctggcag caggaaccct acatgatcaa aaaggcagaa
     4381 aaatctccgg ctccggctat gcaattgaac gaaggcgtgt tgatacggga cggaaaaata
     4441 ctgacgggag ggcgctttaa tcccgcctgg tggagaggca gtctgctgcc taaaaaccaa
     4501 caggctcccc acctgacgcg ttacgctttg gagcccgaag gctaccgggt ggtcgacgat
     4561 ctggacgaag tgtgcgctaa tatgcaaaaa agcggcattt tggtaatgga tcataactac
     4621 ggattatggt acgaccgccg cagggacgac cacgaacgaa ccagccggat agacggcgaa
     4681 gtcaaagccc ccttttatga attacctttc gcccgcagcg gacagggcag agcctgggac
     4741 ggactgagca aatacgacct gacccgatgg aatgcatggt attggaaccg tttgaaaaaa
     4801 tacgcagatt tagccgacga taaaggcctg gtgttgtttc atcagcatta tttccagcac
     4861 aacatcatcg aagccggtgc ccattgggcc gatttcccct ggcgttcggc caacaacgta
     4921 aacgaaacgg gttttcctga gccggtgcct tacgccggaa acaagagggt gtttatggcc
     4981 gaacattttt acgatgtaac tcatccggta cgcagggaat tgcaccgaaa ctacatccgc
     5041 aaatgcctgg ataactttgc agagaattct tcggtgttgc attttatcag cgctgagttt
     5101 accggtccct tgcattttgt ggaattttgg ttggatgttg tttccgagtg ggaaaaagaa
     5161 accggttgta acgcgttgat tgccctaagt accaccaagg aagtgcagga cgctattttg
     5221 aaagaccccg tgcgttcaaa agtggtagaa gcggtcgata ttcgctattg gtatgtggat
     5281 gaaaacggca aagagtttgc tcccaaaggt ggattgaatc ttgctccacg ccaatttgaa
     5341 cgtatggaaa aaccggccaa aacctcggcc ttggaggttt ataatatggt aagcagctat
     5401 cgccggcaat atcctcaaaa ggctgtgatc tattcggccg attcttaccc ggaatttgcc
     5461 tgggccgcct ttatggccgg cgcttctctt tgcgtgttac ccaaagacct gcccgaagat
     5521 tttctgaagg acgcagcttc gctcagcccc cttgctgctg cctgttggct gttggctaag
     5581 gccgggcagg cttatattgc ctatccgtcg gacgatgccc ttatagaggt agatctaagt
     5641 aaggaaacca agcccttccg ggcgcaatgg atagacccgg tcagtggccg gccgaggggt
     5701 aaagcttttc aggtacgggc cggcaccgta ttcaaaacca ctggtaaaga tgttttgtgg
     5761 ctacgccaaa caaactaaaa gaatgtttat tctataaagc aaaatgtatt ctattcaaat
     5821 aacccaaaaa caagctatta tgaaaaagtt tcttgttcct tttttgaccc ttctgttgtt
     5881 tacggcttgt actcccaaag aggtttacat gttcagctca ttcaacgaac cggctaccga
     5941 cggcctgagg ctcttgtaca gcgaagatgc ttatcactgg gaagacctgg gcggtatctt
     6001 tttaaaaccc gcagtcggga atcaacaggt gatgcgcgat ccctctattg tcagggctcc
     6061 cgacggaacc ttccatctgg tatggacctc ttcctggaga ggagatttgg gtttcggcta
     6121 tgcttcgtcc aaagatttga tcaactggtc ggaacaacgt ttccttccgg ttatggcttt
     6181 tgatactacc accgtgaacg tgtgggctcc cgaaattcat tacgacgaaa ctcaggctca
     6241 gtttatcatt atttgggctt cttgtattcc ttaccgtttc gaaaagggaa tcgaggaaga
     6301 aaccggtaac taccgtatgt attacgtcac caccaaagat tttcaggagt tttccgaaac
     6361 caagttgttt tacgatcccg gcttcagcgt gattgatgct gtgatggttc ccagagcaaa
     6421 agacgatgtg gtgttggtac tcaaagacaa tacccgtccg gaacgtaacc tgaaagtggc
     6481 cttcggttct tccatgaccg gcccctttgg cccggctagc gagcctttta ccgagattta
     6541 taccgaagga ccatcggtgg taaaagtggc ggaaggctgg ttgatctatt ttgacgctta
     6601 tcgcaagaaa acctacgaag ctgtgtttac aaaagacttc atacatttcg aaaacgcgaa
     6661 cgatcaaata agtattcccg aaggacacaa acacggcact attttcaaag cagatcagaa
     6721 agtgctgaag gctttaaaaa aagaaatgga aaaacgccga gaagtcgaat cgaataacca
     6781 ataatgtcct caacaatgaa atattctgct ataagcactt tgttgctctt gtttgttttg
     6841 acggtatcct gttcacaaac tgcttccaaa tccggccctc tggcggacag ggtgcattat
     6901 accggaacaa gtatgtctaa tgtagactat caccatggcc aactgtctcc ggccataggt
     6961 gttcacaatg ttcagataat gcgtgccaac cggggcaagc ccgaaagttc ggacggttac
     7021 ggttggacgt acaaccacgc tcccatgatg gcctattgga gcaatacttt ttttgtggaa
     7081 tacctcagcg attccgtcag cgagcatatt cctccctgtc atactttgtt agtccgctct
     7141 tcggacggta taaactggtc aaaacccgaa gtgatttttc ccaagtataa aattcccgac
     7201 ggttttaaaa agccgggcag tgaggtagtg gccaaagacg ccctggccgt gatgcatcag
     7261 cgggtgggct tttatgtttc ctcacataat aaactcctcg cgttggctta ttacggaata
     7321 gctcttttcc ccaaggacga tcccaacgat ggtatgggag taggccgggt agtacgcgaa
     7381 ataaaagccg acggcaccta cgggcccatt tattttttgc gttacaatca atctttcaat
     7441 gaagagaaca gcctttaccc tttttataaa agctgtcccg aagccgattt tgtggcggct
     7501 tgcgatgaaa tactggccaa tcctctgtat atgatgcaaa tggtggaaga agccgatcgc
     7561 gacgacccgc ttattccact ccaaaaacaa tacaaggcct tctcgtatta tcacttgccc
     7621 gacggccggg tggtaggttt atggaaacat gcgcttactt ccattagtgc agacggaggt
     7681 aaaacctggg ccgaaccggt agaacgggcc gggggttttg tcaacagcaa cgccaagata
     7741 tggggacaac gcacttcgga cggcaaatac gccaccgttt acaatccctc ggaatatcgt
     7801 tggcctttgg ccgtttccac ttccaaagac ggactggaat acaccgatct gatgctggtg
     7861 cacggcgaaa taacggccat gcgctacgga ggacagtata aatcttacgg ccctcagtat
     7921 gtacggggca ttcaagaggg caacggcact cctcccgacg gaaaaatgtg gctgacctac
     7981 agtatgaaca aagaagatat ttgggtatcc tcggtacctg taccgatttg tagtaaggcc
     8041 gctaaacata ccagagagat ctttgacgaa atgcccgacg gtcgtgaaac agctttgtgg
     8101 aatacctacg acctgcaatg ggcaagcacc cgcattgaaa aagatgaagc aggtcgtaaa
     8161 agtctcactc tccgcgataa agatcctttt gattatgcta aagccgaaaa gatatttccg
     8221 gcttccgcac aatttacgct gaccgccgaa atcaaagccg gacaaaacga tcacggaact
     8281 ttgcacatgg aggtgcagga cgccaaggga cagccatcca tacgcatcag tttcaacgac
     8341 gacggcagaa tgtacagtaa atccggagcc cgctattcca gcttgggcag ctacgaagcc
     8401 ggtaagagct acacaattaa actgaatatc gacacaaaaa tgcgttacta taccgtgcaa
     8461 atcaattctg gccgcgaagt agggcgtatt ttttatgcac ctgcagccag cctcgaaagg
     8521 gtagtattca gaacaggaga accacgtctg catcctacac ccgaaactcc ggccgaccga
     8581 tacaccgacc tgccggctgc agacactgcc gatcccgagg cctgtttccg tatttactca
     8641 ctggaaacgg ctcacgctac cggtaataat caataatgaa tatattttta tgaaaaaaca
     8701 attagtcgcc tcactgttaa tcggttttac ggtcatttcc tttgcctcct gtcaggcgaa
     8761 aataaaaaca cccaaatcgg ccaatattct caaagccaaa gattttaaac actacgttga
     8821 ttattttaac cgcatggaag atgagcccat cgttcaggcc atacctaatg cggagtcctg
     8881 ggactggatg caggccaata ttcccttgtt cagttgtcct caggataatt tcgaggaaat
     8941 gtggtacttc cgttggtggt cgttgcgtaa gggtattcgc gaaaccccgc tgggttttgt
     9001 tatcaacgaa tttttggtgg aacgttccta tgccgatcaa tacaatatga tttcctgtgc
     9061 gctgggacat catatttatg aaacccgctg gctgcacgat tccaaatacc tcgatcagta
     9121 tttgcatgtg tggttcagag gcaacgaagg ggctcccatg aaaagattgc gcagtttcag
     9181 ttcgtggact gcctatgccg tgtacaacag gtatcttgcc gacggtaaca aagagtatat
     9241 gctcgatttg ttgcccgacc tggaaaagga ataccgagac tgggaaggcg atcaccgttt
     9301 gcccgacggc atgtactggc agggcgatgt gcaggatggc atggaagagt ccgtcagcgg
     9361 gggacgacgc atcaagaatg cccgtcccac cattaatagt tatatgtacg gaaatgccct
     9421 cgctttgtca aaaatgttca gcctaagcgg agagacggcc aaagctgccc tttttgcagc
     9481 caaagccgat accataaaaa aaatggtaca agacaactta tggcatcccg aaaggaaatt
     9541 ttttgaaaca cgttgggtaa acggccaact ggcgcaagct cgcgaagcca tcggttatat
     9601 tccttggtat tttaatttgc ccgacctagg gaaaaattac gaagaagcct ggttgcaggt
     9661 caaagatccg gagggctttt tagctcctta cggtctgact accgccgaaa gacggcatcc
     9721 tgaattccgc actcgcggag tgggtcaatg cgaatgggat ggggcggttt ggccttttgc
     9781 cagctcgcag acaatgacgg ctatggccaa tgtgctcaac aattatgagc aaaattacgt
     9841 ggacaaagcc acctactatg aattgatgaa tctgtatgtg gaatctcagt accatagagg
     9901 ccggccctat ataggcgaat acctggacga agttacaggc tattggttga agggcgatca
     9961 attgcgcagc cgctattaca atcattccac ttttgccgat ctgatcatta cgggcttgat
    10021 gggattacgt ccgggagaag acaatatatt ggtggttaat ccgctgattc cacaggaaca
    10081 atgggattgg ttttgtttgg ataatttgat gtaccatggg aagaatatag ccgtcgtgtg
    10141 ggataaatcc ggaaagaaat tcaataaagg ccaaggttta cttatctttt gcaatggtaa
    10201 aaaagtagct caaaaaccaa ctattgaaaa aatcactgtt gatatttcac cgtactgagc
    10261 taaaccaccc ggacaatttt gtgttaaaac ggtttagtat tacatttgtc ggcttaacac
    10321 tatatattat ggcgtttaag aaaaaagtat ctctgaaaga catcgcgcaa gccgttggag
    10381 tgtcgtccgc tttggtatcg atggtgctca acggcaaggc aaaacaatat ggaatcggtg
    10441 aggaggccac tcgtaaagtg ttggccaagg ccaaggagtt aaactatcaa cccaatatca
    10501 tggccagagg tctcaggagc ggcagaagtc gtctccttgg cttggtggtg gccgatatct
    10561 ccaatccttt ttttgcgact cttgcccgtg aaatagaacg tgctgcctcc gaaagaggct
    10621 acacggtcat ttacggcagt tccgatgaaa agcccgaaca tttgtcgaag cttatgaata
    10681 ctttggccaa tcagggagtg gatggaatta ttgtggttcc ctgtgaaaat tcagaagaac
    10741 tcattgccga tttatacctc caaaagtatg ccatggtttt ggtcgacagg tatttcccca
    10801 aaataaacac tttatcggtg tcgctcgata atgccgaagc atgcaaaaat gttacccggc
    10861 acctcttgtc gcagggttac aagaaaatcg gttttgtaag ttatgaatcg gaactcctgc
    10921 atatgacgga acgcgtaaga ggttactgcg aggctatgca ggaagcgggc ctgaagaata
    10981 gtattaacgt actcaaattg cctcaggata agtatcaaga aacaatgctg caggtttttg
    11041 aacagcatca aatcaaaaac cttgaggcct tgattttttc gaataattcg cttaccattc
    11101 agggtttgta tgtgctcaaa caactaaagg tacgtatccc cgaagacctg gctgtgtttg
    11161 gttttgacgg aggagacgtg tttgatcttt attgtccggc cattagttac gtaagacaac
    11221 cggtcgaaga aatgggcagg aaagccgtaa ctgttttaat cgatcaactt gaatctgaga
    11281 aacccgggga aataaaaagg tacgtattga aatccgactt ggtcattgcc gaatcttctt
    11341 taaaaaagaa atagatgaat caagaagaat acaagaggtc cgttaatcat cagaaatatt
    11401 gaaccctaaa ccattaataa tctaaaacga atgaccaaga atttttccgg agtggtggta
    11461 cctatgatca ctcctttgct gaatgaccta agagtggata ctgccgcagt taagcgtatt
    11521 atgaagcttt tttcggccca ttccatatct ccgctggtat tgggcacaac gggtgaatct
    11581 tcttccgttt cgaggaagga aagtttgcgc tgcatacgcg cagcctgcag agcaaaaagc
    11641 ggaacccaaa aagtctatgc cggattggta ggcaatgaag tgggcgaatt gttggatcgc
    11701 ggcaaaatgt atgccgatat gggtgttgat gccgttgttt ctaccttgcc gtcgtattac
    11761 ccgctttcgg cagatcaaat gcgttctttt tacctgacct tggccgattc tttatccgtg
    11821 ccggttctca tgtacaatat caaggcaaca acccaaatgt cgctttccct cgatttggta
    11881 gccgaattga gccagcatcc caatattgcc ggattgaaag attccgaaag ggatcccgca
    11941 cgtttgaacg aatgcataca gaagtataaa aatcgttccg atttttcctt tttctgcggc
    12001 tggggtgcgc aaggagcgaa tagtttgata ctcggagccg acggtatcgt ccccagcaca
    12061 ggcaatgtgg ttccggaaat gtattataaa ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nngccaggat ttacagctcg ttggctttta ttctgaccca cttttcgaaa
    12241 atgggcactg tgttgtattt ggtcagtctg gcacttcaca gcatgatagg tgtcaatgtg
    12301 atttgggtta tttggttttt gggtatagcc attgtgttgc ttactctgtt agggggtatg
    12361 gagggggtga tatggatgga cgttattcag ggcattttat tgatatgcgg aggcctgctt
    12421 tgtgccggaa tattgctttt caggcccgaa ggaggacctg tgtcggtcct tacggaagcc
    12481 atcactacca agaagattgg ctttggtccc tatgacttca attttgccga attgactttt
    12541 tgggttatgg ctatcaacgg gattttttat gccttgcaaa aatacggcac cgatcagact
    12601 atagtacaac gctatctgac tgccaaaacc gacagagatg ccaaaaaagc ggcttacatc
    12661 ggagtaggcc tcagtgtgcc catctggacc ttgtttatgt tgataggtac tgctctgtat
    12721 tttttctatc attccggaac agccgtactg cccgagggtt tgaaagcaga cgaagtcttt
    12781 ccctatttta tcataaacga actacctgta ggtattgttg gtctggttat cgcagccttg
    12841 gtggctgctg ccatttccag cctcgattcg gatatgaatt gtctggcagc catcggggtg
    12901 gaagattatt atcagcgtct aaaaccaaac tgcaccgata agcaacggtt gaatatgggc
    12961 agaatattgg ttgccgtttc gggcttggcc tccataggtg tagccagtct ttacgccgtt
    13021 tgggagggtg aaggagtgct gggtgtgatt tttgaattat acgccatttt ctctgccggg
    13081 atagtcggta tctttctatt ggggcttttc agcaaaaggg ccaacaaaca aggcttgtac
    13141 atcggcatgc tggtttgtgt gctctttacg gcttatgcgg tactaaccag cacatccgtc
    13201 gaggttggag gcagcaaacg tctgattctc gatttgggct caatgaactt tccccatcat
    13261 aagtacatgt tgggcgttta cagtcatctg attgtattgg tagtgggtta tgtggccagt
    13321 ttgtttttcc cgaaacccca attggaaaag ggcctgacca ttttcgatta caaaaagccg
    13381 ggcaaaaatt aactgattat gagaagcatt gaactgatac aacccaaaca tataatcttt
    13441 ggtgccggct atgcacaaga ctgggcaaat attgtattgg ccgggaaaaa acaaagggtg
    13501 ttgcttttat ctactcatcc cctttgggct gttttagaaa aaagcatcgg gaacgcgcga
    13561 gaaaaaggat tggagttgtt gtgtctggat tattcctggc aagccgagcc cgacacggaa
    13621 atctttgaaa acttgtaccg gcaaagcaaa gacttcggcc cccaggcaat cgtggccatc
    13681 ggcggaggca gcgtgctcga cgtgggcaag ctgctggcag ccctgtcttc gggtacccaa
    13741 aagccctccg atgtttttgg taaaaatctg ctcgacagcc gggacaatct gttgatttgt
    13801 gttcccacca cggcgggaac cggcagtgaa gtatcgccca acgccatttt gttcgatttg
    13861 cgcgacggtg agaaaaaagg catcatcagt ccatggctga taccagatac ggcattgatc
    13921 gatccggaga tgacttttag cctgcccccg aaactcactg ccgaaacggg catggatgct
    13981 ttatgccact gtattgaggc ttataccaac aaatacgccc acccggccgt agatctgtac
    14041 gccctcaagg gaattgagta cgtgtacaaa aacttgaaaa cagcggtaaa ggagggtaac
    14101 aatatcgaag cccgctctgc catggccttg gcttctatgt atggaggact ttgtctgggc
    14161 ccggtgaata cttcggccgt gcatgcctta tcctacggcc tggccgggaa atatcatttt
    14221 tcccacggct tagccaacgc cgttttgttg cctgccgtaa tgttgttcaa catggaagcc
    14281 gatttggaaa aaaatgctcg aatagctttg gctatgggtc ttccggataa aggagatctg
    14341 agagcgacgg ctcggtcggg aataaaagcc atacaagagc tttcacgtga ttgcggtatt
    14401 cctcaaagct tgtcttcgct gggagttaaa cgggaagatt taccgcagtt ggcagacatg
    14461 gccatgaacg tcaagcgctt gttgaataat aatcccagag aacttgagtg gaaagatgcc
    14521 ctgagtattt atcaatctat actatgatcg caatgaacaa aacaaataat accaaacccg
    14581 ttataggaat cagcatgggc gatcccgccg gtatcggccc cgagatcatt gtgaaagctt
    14641 tatcttcagc ggaggtttat gccgcctgcc ggcccgtggt ggtaggagat gccggagtta
    14701 tgcgacaagc cgtacaatta ctgggcaagg acctgaaaat tcatccggtt caaagcattc
    14761 cggaggctcg ctttgagtac ggcaccatag atgtgtatga tttgcataac gttgatttaa
    14821 gcaagttaca acacggtgtg gtatctgcta tggcgggcgg ggcggctttc gaggcggtgc
    14881 gttgcctgat cgatctggcc atgacttctc aggtggacgc cactgttacg gcgcctatca
    14941 acaaggagtc tatccacaag gccggacacg ccttttcggg acataccgaa atttatgccc
    15001 attacaccaa caccaaaaat tacgccatgc tgctggccga tgacgccctc agagtgatac
    15061 acgtcagtac tcacgtgtct ttgcgtcagg cttgcgaccg cgtaaccaaa tcgagggtgc
    15121 tcgatgtaat agagcttttg aacaaggcct gcgtgcaatt nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnnnnnnnnn ttgctgggat ttaagtggaa taatgaaacc cagaaaatgg
    15301 atagtgttaa aggcgtcaat ataaccttgg gcttacccat catcagaacc tctgtggatc
    15361 acggcactgc ctttgaaatt gcgggcaagg ggatagcctg cgaagacgct atgctcattt
    15421 cgatcgatta cgccttgaaa atggccaaaa atagaatcca atgattgtca ttattgcaga
    15481 cgatttaacc ggagcagccg aaaccgtcgg tatctgtttg cgtcacggag tcggggcggc
    15541 catgattatg gaaatcccgg gcgaagagcg tttgcaggaa tatacaaaag agggaatagc
    15601 tgtattggta attgcctgtg atacaagatc cggcacctta gaagaggcct gccgggtaaa
    15661 ccgtttactg ggtgaaacgc tcaaacaggc cggattcacg caaatcttca aaaaaatcga
    15721 ctccgtattg cgaggctggg tagtacccga actgaataca ctggcccgat tgacgggcaa
    15781 aagcagtttg ctgatccagc cggccaatcc cggagccgga cgttgcattc gcgacggaaa
    15841 gtattttatt aacgggattg aattaagcga aacggctttt ggcctggatc ctgagttccc
    15901 tgcctcttcg tcggatgtaa tggagatact gcaagagcgt ctgcccggaa aagagccggc
    15961 ctcggtaata aaattcagta ttccggatgc ctgttatccg gaagatttac gcaacagcgc
    16021 cctgcaatgt ggcccggatg ttttaccggc cggcagtgcc gctttttggg aggcctatct
    16081 cgaggagcaa atcaagcggg gcctgatcct taccgccggt atcaattacc gaccccatac
    16141 acaacgtaaa aagccgcctg ttccttcgac acacaatttc ttgatgatct gtggcagtgc
    16201 tcatcaacac agtctggatt acgacgaata tttaagaaag cagggggtaa ctctgatgga
    16261 aatgcccccg gaattaagtt cggaacaaga accggatccg gccgatccgg ctgcctgggc
    16321 gagtgcctgt gtggaagcct ggaaaaaaga accccgtttg gctctaagga ttgcctctaa
    16381 accaatcaac tacgagaaca gcgcccacaa actcaaagct cgttttaccg atgccttgca
    16441 attgatttta cagcaaatca aaccggccga attattcatc gaaggaggtg cgacggctgc
    16501 gagtctgctg aaccggctca actggacgac atttaaaccg gtggacgaat tggctccggg
    16561 agtggtccgc ttacaattga tgcctgaacc ttctcttttt ataaccttga aaccgggcag
    16621 ttatttctgg ccggaattct tattcaattc atagacaatg aaaaatctga tcagcctggc
    16681 atggcttctt ttattgcctt tttacgcgga agcaactact gtcaacgcac tcaaagccgg
    16741 cgcaaaaaac gatggcaaaa cactgaatac caatgctatt tctaaagcaa tcgataaact
    16801 gagttcccaa ggtggcggca ccttgtattt tccttcgggt acctatctga ccggtcccat
    16861 aaggctccaa agcaacatta cgctcgattt ggatgccgga gccgtgttgt ctttttcgga
    16921 tcgtttcgac ttatatatgc cttacgtcga aatgcgttac gagggagtgg tgatgaagag
    16981 ctttcatcct ctgatttatg cttacgaagc cgaaaacatc actataaagg gcagaggaag
    17041 actcgaaggt aacggccggg cttggtggga agagacctgg cgactggaaa cggcgttcta
    17101 caagggagaa ttgcaagacg ccgaaccgac caaataccag caagcctggc tggatgccaa
    17161 tcccggtttc agtgtcgaac cccagtcgga ctggaaaaac accttggcca ggcattggtt
    17221 cagaccgcct tttattcaat tgtacaaatg caaaaacatt ttgatagaag gtctgcacat
    17281 cgctaattct cctttctgga ccgtgaatcc ggaattttgc gataatctca ccatccaggg
    17341 cctgaccatc gtaaatccga attcccccaa caccgacggt atcaaccccg aatcctgtac
    17401 caatgtgcat atttccaatt gccatatcag cgtcggcgac gactgcatca ccatcaaatc
    17461 gggtcgtgac ctgcaagggc gtcaatacgc cgctccctgc gaaaacatca ccatcaccaa
    17521 ttgcaccatg ctttccggtc atggcggcgt ggttattggc agtgagatgt cgggagatgt
    17581 ccgcaagatc accatcagca attgcgtgtt cgacggcacc gaccgtggta tccgcttgaa
    17641 gtcaacccgg ggcaggggag gcatagtcga agaaattcgc gtgagcaata ttgtgatgaa
    17701 gaacattcaa aaagaggcca ttattctaaa cctgttttac agcagggtgc ctcaggagcc
    17761 cgtgaccgaa agaactccca tttttagaaa catccatatt tcgggcatga ccggctcgga
    17821 ggtgaaatcg gcaggatcga ttctcggcat tcctgaaatg cccatttcca atattagcat
    17881 agaagacgtg aatatccagg cagaaagcgg tttcagtgtt caggatgccc gggacattca
    17941 attgcgcaat atatgcatcg acacccaaaa agggccgatt gtacaatgca aggatgcagc
    18001 cgaattgtat ttgtccaata tacgaagctc caaacccttg gcggaggccg ctttgctaag
    18061 gatggaaaac gtcagcgacg tctttattga aggctgtttc cccttgcccg gaagcaaagc
    18121 ttttttggaa ttgtccggtg cggaaagcag cagggtgata ttgaaaaata attttgttga
    18181 acgcattgaa caaccctacc ttattcatga aatggtcgat tctgccgcat tggtttattg
    18241 atatgaaaca ttcctgacgt attctacatc cgagcgaatg aaacagaatt gctaacatta
    18301 aaaattcacg tatgaacaaa atctggctta cagtcttatt gtgtattagt gtctgcgcct
    18361 attctcagca gaattccgac aattgcttcc ggcgtcccct caaagaggtg ttgacggatt
    18421 tgcagtcgca atttggcgta cgtctgcgtt tttccgaaga actggtcagg gacaaagtgc
    18481 tcgattacgc cgattggcgc attcatccct ggagtctgga agaaagcctg aacaacgttt
    18541 tgctgccttt tgactataaa tttgtcaaag aaagcagtac ttcttacgaa atcaaagcct
    18601 tcgagtatgc gcgcagaacg gctgatttcg gcagggactt tttagattac ctgagtagcc
    18661 ggcacgccga tttgcccgcc tgggaagctc gtaaggccct gctcaaaaaa gaaatgaaga
    18721 tcgctgccgg cttggatccg ctgccccgga gtcccgggac taaacctatc ctgggcccta
    18781 aacgtaaata cgaaggctac tacgtgcaaa atatagccct ggaaatttta cccggcgttt
    18841 tttgtacggg ttcaatatac catcccattg gaaacaaagg caaatgtcct attatgctca
    18901 atcccaacgg tcattttggt gatggccgtt atcgtgccga ccaacaactg cgctgtgcca
    18961 tgcaagccag gatgggcatc attgcggtca gttacgatct ttttgcctgg gatgaacagt
    19021 tgttgcaatt cgacagcaga agccatcgca gcagcatggc ccacaccata caaagtctga
    19081 acgccattag aatgctcgat tatctctgtg ccttgaaaga agccgatctc agcagagtgg
    19141 ggataaccgg cggatcgggc ggcggcagcc aaaccatgtt tattacggcc atcgacgaga
    19201 ggattaccct gagtatgccg gtagtgatga cttcgtctta ttttgtgggc ggctgtccct
    19261 gcgaaagcgg caatccgatt catctttgcg ggggcagaac caacaacgct gagataaccg
    19321 ccatgtgtgc tccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgggttt
    19441 ttgaagcagc caaacaataa acaacttcaa ggacagcaac aactccaggg aaaacaccga
    19501 ccccaaggat agcaaaacca atggaagaaa caacaccaac tccagggaga ccaaaacaat
    19561 ttaaagtaca aaaaagcaac tctaaggata ccaaaaacaa aaagatcatc ctcaaaacaa
    19621 taaaagataa aactatggta aaataccgga tattcgctct ttgtttgata gttaccggct
    19681 tgttttatgc ctgtcaatcg cctgagatcg atttgcaagc cgagcatctc cgactggact
    19741 acaacggcgg ggaaggggcc cggcagcttt gcctgagttg gacgatgaat gcatcctctc
    19801 ccagaggatt gaaccaacaa gcctaccgta tacgtattgc cagccgtcct gaactgctcg
    19861 aacaaaattc gcccgatatt tgggattcgg gtaaagttag cagctcaaag aattacgatg
    19921 ttccgtataa aggtaaagcc ttagagagag ataccgtcta ttatgcccaa ataaggatat
    19981 ggaccaatcg gggcaaaagt ctctggtcca aacagcaaaa cctggttttg ccggccgaag
    20041 gagatcttct ccccgctgct gcatccatta atcgcttcga cacctacgac ctgaagttga
    20101 gcgacctggt tcagacatat aaaacagcca ctcttgagaa atacaagaat cggccccaag
    20161 acttggaaaa catgtcagcc gccgactgtt gggccgaaag ttatttgatc gataaccgca
    20221 agttttatct ccaatggatg gatcgcttgc tggctgctca agccgaagac ggtagcctgc
    20281 ctttgtctgc gcaacaggag ccgcaggaaa ttttcgatat tgcccatatg ttgttggttc
    20341 agtacaacga aaccgctcat ctgaagaaac attttccggc gatggaaaaa tggctggact
    20401 ttttgtggaa aaactacgga agccaagagt atatactgac agccaatgga gatggacaca
    20461 ccctgttgcc tgtcgaagaa tatagtctgg atggaaaaga gtacatcacc tcggctgctt
    20521 tgatttcgac ggcgtatttt tatcgcctct gcgtgttgat gcaaaagttc tcttctcacc
    20581 tacagtatcg ggataaagcc gctcattatg ccgattgggc cggcaagctt cgtaaagcct
    20641 acaacgaccg ttttttcaat gaagaaaaac aatactacga aaacaatacc ccgacggcca
    20701 atttattgtc cctaagctta ggattctgtg aagataagag ggcggagagt gtattcaaac
    20761 gcatagaaca atccatagct atagagcatt acggaaaagc ggcttgcggt gaatttggca
    20821 aaagatattt gtaccgcacc ttatcggcct ggggcagatc cgatctgagt tacagaattg
    20881 gggctgagct ccgggaggtc gagggcgatt ttattctgtg gttttttgag catctatgcg
    20941 gtttgaaaag tctggaagag ggcttccact gtttcagcct cgatgctgtc ttccccccgg
    21001 gaatttattt tgccaagacc cgtatggaaa cccgcggagg aattatatcc agttcctgga
    21061 taaaaacctt cgatgaattt gtttggcagg tgatcgttcc tgtcaatacc agggccagac
    21121 tccgctatcc ggctgctgcc gtcaaagaca tttacgacga cggccgggta gttgatcagg
    21181 tagagggcat tcaaattctt ggtcgggaaa acggaaatat acttatggaa ctgggctcgg
    21241 gcgattattt tttctccagt aaattggccg acattaaacc aggcccgcgt aggaacaatg
    21301 aatgatttat atccaacctt taattttata ttgtatgaaa acaaaaagta tgctattgac
    21361 aatgggcctt ttgctcagct ttacggcctg ccggcaagga gctgaatgga aaaagggaat
    21421 ccttatagac gaatttgttt acgaaaaaac ttcctttccc gaatgtcact ctgcttcgtt
    21481 ggcagaaact cccgaaggta ttgtgttgac ttttttcggc ggaaccaaag aacgccaccc
    21541 cgatgccagt atatggatat gccgaaacga aaacggtgtt tggaccgaac ccgaaaaagc
    21601 ggccgacgga gtagtcaacg atacattgag atatccttgc tggaatcctg tgttgtatca
    21661 agttcccggc gaagagttgc tgctgtttta caaggtcggc cccagtcccg gatcctggtg
    21721 gggttgtatg aaacgttcct tcgacaacgg taaaacctgg tctgccgagg aaaaactgcc
    21781 cgagggcatt atcggcccgg tcaaaaacaa accggtggtg gtggacggaa aattattctg
    21841 tccttcgagc accgaaaacc gcggatggag actgcacatc gacgttaccg aagacaaagg
    21901 caaaacctgg acacagatag gccctttgaa cgatccggca gaatggctgg ctattcaacc
    21961 aagtattttg tttcacaaaa acggagattt gcaaatactc tgccgtaccc gtaaccgcct
    22021 ggtgggttcc acctggtcaa ccgaccgtgg ccggacctgg tcgcagttgg aaggtgtagg
    22081 tttgcctaac aacaattcgg gaacggatgc cgttactttg aaagacggcc ggcatctgct
    22141 ggtttataat cacgtggaac ctcctgccgg tgagacaaaa ggagcccgaa cccctctgaa
    22201 cgtagcttta tcgaaagacg gaaaaacctg gtacgcttcg ctggtgctgg aagattcacc
    22261 catcagtcaa tattcctatc cctgcgttat tcaatcctcc gacggtttcg tgcacatagc
    22321 ttacacctgg agacgccagc gcattaaata cgtcaaaatc gaccccgcca aacttaaaat
    22381 gaaagaaata gaaaacggta tctggcccgg caaggaaaca gcccaggccg ttgagggtgc
    22441 aggctctacc ccttttccca tttcggtttg cgactggatg atattaaagc gccagaaact
    22501 cggagccatc actctggcca gagaaatagg ctgcgacggc cttgaaatag acatgggtgg
    22561 cctgggtaat cgtcccactt ttgacaacaa actcaaagat cccgaggtgc tggctgaatt
    22621 caaacgtacc gcacaggtga gcggaattcg cttctcctcg gtggccatgt cgggttttta
    22681 cgctcaatca ctggtagatc gcgaagattt tatcacaccg gtgcaggatt gtatcgacgt
    22741 aatgaaaggt ctgggtgtag gagtagcttt tttgcctttg ggtgtaaaag gagacctggt
    22801 gcaatatccc gaattgcgcc ccgctatagt ggagcgcctg cgagttatcg gccggatggc
    22861 cgaacaggaa aaggtggtca tcggtataga aacctctttg gatgctgagg gcgataaagc
    22921 attattgcag gaaatcggca gtccggggat caagatttat ttcaatttcg caagagccct
    22981 gcaaagcggc aaagatttgc atcgggagtt gcgcttgttg ggtgacgata tatgtatgat
    23041 acattgcacc aacaaagaca gcgtatggct ggaaaacgac cctatgatcg atatgcctgc
    23101 cgtcaaacag acactcgaag aaatcggtta ccggggctgg ctggtaatgg aacgttccag
    23161 ggatgcaagc caggcccgga atgtccgtgg aaattacggg gccaattgtg cctatctaaa
    23221 gtcggttttt caaccggaat agtatggttt aaacgcagat ttgaacttgc aaggttcagc
    23281 agctttaact tgtttttcaa acgaatagta tggtttaaac ccgctttgat gcgaaccatt
    23341 cagaaatatt tattaagtcc aggaacacta cagttatgat caaaaaatct ttcattgtat
    23401 tagctctgtt atgtagctct ttgtcaattt ttgcacggac ggaaatttgg gtagctcccg
    23461 aaggctcgga ccaggctccg ggcaccaagg cggaacccaa ggccagtttg cattcggccc
    23521 tgaggcaggc aagggaaagt agaagattgt ccgatccttc gacagcggac ggaatcatta
    23581 ttcgaatgca agcgggaact tattttttgg acgaacctct cctgataaga cccgaagatt
    23641 gcggaactcc tgagagtcca acccgtatag tggccgaaaa aagcggcgaa gtgacactga
    23701 gcggcggagt aaaactgggc aaatggagca aagccggcag cgttccgggt ttaccctcgg
    23761 ctgccaaagg caaggtctgg gtaacagatc agcctaaagt ttcaggccgc tacatcgatt
    23821 ttcgacaatt atgggtagac ggtcgtaaag ctgttcgggc cagagatacc aacgacgaca
    23881 attttaaacg tatattggcc tgggacaaaa acacaggggt gttaaccatt cccgctcctg
    23941 gagtaaaatc ctttaaaact ttggatcgcc tcgaattgca gctgcatcag atgtgggcaa
    24001 tagccaattt gcgtataaaa gccatagaat atcaaaacga aaaagctttg gtggtttttc
    24061 atcaacccga aagtcgcatc caggccgagc atccatggcc aaccccgatg attgcggaag
    24121 gccgggaatc tccctttttt ctgtgcaatg ccattgaatt tctcgatcag cccggggaat
    24181 ggtacctcga cgtggaagat cataagcttt attattggcc tcgcccggga gaagatatca
    24241 cccggaccga atcggtggtt ccttatttgg aaacacttat agaggttcag ggcagtttgg
    24301 atcgtcctgt aaagcatata agcttcgaag gtatcggctt ttcgtacaca agctggctga
    24361 ggccctcgga acaggggcat gttcccctgc aggcaggtat gtacctgctc gatgcgtata
    24421 aattgcgtcc tcccggcgta ccgggcaacg aaaaccgggg tttggaaaat cagggttggg
    24481 tgggtagacc tccggctgcc gtacaattgc gtggggtaca aaatacgagc tttgtgcgtt
    24541 gccgttttga gcacctggcc tcatgtggga tagattatat cagcggaact ctgaacgata
    24601 gtatattagc ttgtgtcttt caggatattg gcggtaacgg catacaagcc ggaaagtttt
    24661 ccgatccggg catagaaaca cacttgcctt acgatcctgt cgatcggcgc gagatatgta
    24721 ccaaactgta catcggaaac aattacattc acaattgcag caacgaagac tggggttgtg
    24781 tgggtatttg cgccggttac gtgagggata ttctcattga gcacaacgaa ctatcggaat
    24841 tgtcttattc gggtatcagt ttgggttggg gttggagtaa aaccgtgaat tgtatgcgca
    24901 acaatgtggt gagagccaat tacattcatc attacgcaaa acgtatgtac gatgtggccg
    24961 gtatctacac tctgtcggtg caatcaaaat ctctgataag cgaaaatgtg gtcgacagta
    25021 tctattcgcc ggcctacgtt cacgatccca atcactggtt ttatctttac accgacgagg
    25081 gctcctcttt tattacggta aaagataact ggtgtccggc cgagaaattt ctgcagaacg
    25141 ccaacggccc gggcaatacc tggattaaca acggtccgat ggttgacgaa aaaatcaggt
    25201 tggcagcagg cattgagccg gcatacagcg acataaagcc ctgatattgt ccggtcgttt
    25261 ttattcatcg cccaccaagg cgagggcaat actcatcaga acttgcacca tacgaatcct
    25321 aataaagcgt gtatatttat gcaagataat gcaaagcgaa cagtactaaa attcagtgag
    25381 ttaaaattcc tgtgaaacta ctccaaactg ttttttagcc tgttttacgc tccaattaaa
    25441 caagtaaatc gcttatttag cacctattta ggctttaaaa acgttaaatc ttttttgagg
    25501 atattttttt tgtcttcctt tgttacctcg gttgtatatt tataacaatt agttgttata
    25561 aaatataatc gcttattaat cagcaatttg tgttttccac aagaataaaa ataattgtta
    25621 atcctaaaga attctctctc agttttagcg tattatccct ttaaaatatt gcttaaagaa
    25681 tcacttaaac atttgcgtaa taaaaaagct taaattacat ttgcgctcca aagtgattaa
    25741 ctttacggaa gttaaaccct aaacagaaaa tgattgttaa acaaaaagtg aaactatgag
    25801 aaaactgact tttttactcg cttgcctgtt tctggcaagc gtaggcttag tgaatgctca
    25861 gtctcgcacg gtaaccggta gggttattta tgccgaagac gagcagcctg tcattggtgc
    25921 gtacgtagtt gttcaaggca gctccagggg taccgtaacc gacgctgacg gtaatttttc
    25981 catcagtgtg cctcaggaag caaccatttt ggaattttcc tttctgggaa tgaaaacggt
    26041 acaagccgaa gccaggaacg gaatggtggt gcgcatggag caggaagtgt ctgaattgga
    26101 cgaagtaatg gtcgttgctt tcggtactgc aaccaaaaag tcttttacag gatcggcagc
    26161 agtaattaaa gcagaagaca ttaccacacg gcaggtttcg aacgttacca atgccctggc
    26221 cggtcaggtt gcgggtgtgc agacagttaa caccagcggc ggtcagcccg gtaacgcagc
    26281 cacaatccgt attcgcggta tcggctctat gagcgccagt aatgctccct tgtacattgt
    26341 ggacggagct ccttttgatg gcagtatgtc ttcgatcaat ccccaggaca tcgaaagcat
    26401 cagtgtgttg aaagacgccg cagccaacgc tatttacggt gcccgtggtg ccaacggtgt
    26461 tgtgttgatt accaccaaaa gaggtacgac ccaggaagcc aaagtgaatg tggacgcaaa
    26521 atggggcaac aatagccgtg ctgttcccaa ttacgctgtt atggaagatc ctgccatgta
    26581 ctacgaaact ttctacaaag ctttgtataa cagtaagatc tataatggaa acagtgctaa
    26641 tgatgcccat gtttatgcca ggagcgtcct cttcgataag gatgccggag gtttgggcta
    26701 tcaagtatac acccttcctg cgggtcaaag cctgatcggt accaacttca aactgaatcc
    26761 caatgctacg ttgggttaca aggatgccga tttttattat acacccgata attggtataa
    26821 cgaaattttt gatcccaaca acttacgtca ggagtacaat gtgtccgttt ccggagcgtc
    26881 agacaaaatc aattatttta tgtctttagg ctatctggac gattcgggta ttgtatcggg
    26941 ttcgggcttt tcgcgctata ctgcccgtac taacgttgaa tatcaggcta aagaatggtt
    27001 aaaagtggga gccaacattg gttacaccaa ttacgacatc cagtctcccg gctctcaaac
    27061 caactggggg tccagcggaa acctctttta cgtaaccagt ttgatagccc cgatttatcc
    27121 tctgtatgtt cgcaacagca cagacgagca gattcgtgtt gaccacagag gcattaccgt
    27181 atacgatttt ggtactacta ccaaccagaa acgcgctttt atgggtatgt ccaatccggc
    27241 cattacgctc aagctggatg aacaccatgc ttataccgat gtcttggcca gccgtttcta
    27301 tggaaccctg gatttgttta aaggtatgaa gtttaccgcc agtgtaagcg ccaataccat
    27361 taatcaacgt gcgaatcgcc tgtataacca attctatggt gccgctgtcg ctagtcaggg
    27421 tgccgtaagc gtttcccatg caaggggtgt aggaattaac caacaataca tgcttaatta
    27481 caaaaggtcg tttggtgtaa acggtcttga tatattggcc ggttacgaat cctatgaatt
    27541 gaccgagcaa tacctttacg gctataacac caagctcttt aacccttata ttggagaatt
    27601 gggtaatgcg atagacgttg attcaaggga tatgacatct tataccaatc attactcaac
    27661 tttaggtttt ataggccgcg cagaatacga ttacgatcag aaatacatcc tttcggcttc
    27721 ctatcgtcgc gatgcctcct ctcgttttgc tcccgaacag cgttggggtg actttggaag
    27781 cgtcggtgct gcatgggcaa taaacaatga aggatttatg gccaatgcgg agtctataaa
    27841 tttactcaaa ctcaaagcca gttacggtat ccagggtaac gataacattg gaaactatta
    27901 tgcttacctc gatcagtaca ccgtcagcaa tgctgatgga gatttttcgg taggcttcgc
    27961 gtataaaggt aatcccgaca taacctggga aacttcctat agtttcaata caggtataga
    28021 tttttccatg ttcgacgaaa ggttgaacgg tacgattgag tatttctcgc gcaagaccgt
    28081 agatttgctc tacaatcagc cggtgcctct ttcgtttggt tatgcctcca tacccaccaa
    28141 cgtgggggct attctgaaca atggggtaga gattgatttg cgttacactt tggtcaaaac
    28201 caacaatcta acctgggaca tcaatgtgaa tgccacccat tacaagaaca ctattcttga
    28261 tttggcagaa tccgtcaaag aggccggagg tatcaagcaa agcacctaca tatacagggt
    28321 gggcggatct ctttataaca cctacctgag ggaatatgcc ggagttgatc cggttaccgg
    28381 taaagcattg tattatatgg atcccgataa cgacgattat tcaactaccg acgtctggtc
    28441 gtccgccaaa caagccgaca acggaagttc tttggcaaaa gtttatggtg gattcgggac
    28501 agctttacaa ttgagaggca tcgacctgtc ggccaatttc tcttaccaac tgggagggaa
    28561 actttacgac ttttcgtacg aagagctgat gcatagtggc gacaacgccg gtttaaactg
    28621 gcatacagat attttaaatg cctggacgcc cgagaatacc gttaccgatg ttccccggat
    28681 ttgttcttcc gacgatactt atcaaaccta ttccacccgt ttcatgatca gctcaaatta
    28741 cttgagcctg aacaatatta ctcttggtta taccttacct aaacgttgga cggaaaagat
    28801 caaaattgaa aaattgcgta tatatgccgt aggcgataat ctggccttgt tctcggctcg
    28861 taagggtttt gatcccagaa ccatgatcgg tgccggcgta ggttcgcttg gtacggcagg
    28921 aacaggttct catacttaca ctgcactccg gaccatttcc ggaggcatat cccttacatt
    28981 ttaatacgtc acattataaa actgtataaa gatgaaaaaa cgtaatattt tctttttagc
    29041 gctcggattg gtgttgctgg gatcttgtac ggatctgaat actattcctg agggaggtac
    29101 tcttaccgaa gaacagaaaa gggaggtggt cgaagctatt cccggccgcc tggcagcaga
    29161 catcaatggt atgtttgcgg tcctttcgaa acaatattgt gtatttggag cagctagtgg
    29221 acgtgatgat gaccacggct atcctgcggc agccatgtcc tgggatctga atggccccga
    29281 tatggtaggt acagacgata attacaactg gttctccgtt gcttccgatt atgccgacag
    29341 gacctatact tatgccaatc cctatatgag gtgggccatg ttctataagc agatcaaaat
    29401 ggcaaacgac gtaatggcgt ctattccaga agataccgat aatccgactt tgttgaccta
    29461 cagaggtctt gctcatgctg ttcgtgcctt tgattatttc tcgttggttc aatactacca
    29521 atttacctat ggtccggaaa ctttggataa accggctgtt cctattgtag agcccggaat
    29581 gaccgatgta accaatccca gagcaaccgt acgacaagtg tacgaattga ttatgaacga
    29641 catcgacacg gccattgttt tactcaagga ttacgtgccg acatccaagt cttacatcag
    29701 cgaagccgta gcctatggtc ttcgtgccag agtcaatctc gtaatgaaaa actacgctca
    29761 ggctgcattg gatgcccgta aggccattga tttattcgga ggaactcctt ataccagaga
    29821 ggaacttatg actcccggat ttatagacgc aaccgaccac aattggatgt gggctttaat
    29881 cattactccc gacgtggtgc ctgatgctta tcctgcgtgg cccgccgtac ttgcttcctt
    29941 ctcgggcgac agttatacct gcggggtggg ttgttacaag atgattaata ccctgttgta
    30001 ttcgattatt cctgccaccg atgtacgcaa aggctggtgg gtagatgccg aattgaaatc
    30061 acctctcatc gacagtctgt catggcccga atacaaaggc gaacccctgg gtccgttgaa
    30121 cattcccgat gtaaaaatgc cttatttgcc ttataccaat gttaagtttg gccaatttgg
    30181 tggaattgga aacacagtaa atgcgggcga ctggtgtatg atgcgtgtag aggaaatgct
    30241 cttgattgaa gctgaagcta caggtatgac tgacgaggcc gccggaaaga atttgttgac
    30301 aaattttgtg aaaacctaca gagatcccgc ttataccttc cccgcctccg gtattaaatt
    30361 ccaggacgaa tgctggaaac aacgccgtat agaactgtgg ggcgaaggtt tctccatgat
    30421 ggatattatg cgtctcaaga aaaacattgt gcgttttaat tcgagggttg aaaccaatca
    30481 tccggaagct ttccgtttca acctggctgc cgacgacggc tggttgttga tgcgtattcc
    30541 ccaaagagaa atcaactcga accctggtat cggcgatgaa gacaacaaca acgatggagc
    30601 tttgcccgta agcggtgacg gtgcaggcct tagagatggt gtaacagact aattaaacaa
    30661 gaatcattta atcatgcacg ctatgaaaaa ttttaaaaga atattcttta tcatagggct
    30721 gggggccttc ttatggtcgt cctgtaccga ggatattccc gtaagggaag aatctccggc
    30781 ggctgacccc gaaagtatgc aagtatactt ccccaacagc caaaagtaca aatatgaatt
    30841 gcctccgacc gttttcaaag tcgatattga tctgaaacgc gaaaagtctg cgaaggctgc
    30901 tacggttaaa attatagtga cctccgattt gaccggcgtt tttaccgtac ccgaaactgt
    30961 tagttttgcc gcgggcgatt ccacggccaa gcttaccatc accttcgata ctcttgaaca
    31021 gtttaagccc taccagatat cgttcaagat agaagatcaa tacatcaatc cttatgttgt
    31081 taaccccgat ggtactacag actttaacct gtatgttgag cacaacgact tcctgcctgt
    31141 gggtaacggt aagggtcaat tcttcgacag ctttaccttg gcctcagttg ccgatgtatc
    31201 tatcacttac tctgagaaaa aggacgttta ccgtattagt aatccctata ctctggctct
    31261 tctcgaggaa gccgaatggg gaaactggat tggaggtcct ataagcgaga atatagaaat
    31321 ccagataacg agcgaaggga aagtaacctg ggagttctgg tatcttggat tgaattatca
    31381 gggtgtcagc ggatatccta taaaagctta tttcccctca gtccttgatg aatcactagc
    31441 tgctcttgac gacaagagtg ttaagcttca ggataagctg ttcaaactgc atccttattt
    31501 ctacatagac ggacttggtg gttttggcgc tgattatccg gtatttattt ctttccccgg
    31561 tggccccaat cttaatgaac ttttggcaca ataatcctaa gtttctgaaa ccatagaatg
    31621 aaagcccttc ctgaatcagg aggggctttt ttttagagca tctttcgggg caggcatctt
    31681 tcggggcagg catcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcatct
    31801 ttcggggcag gcatctttcg gggcaggcat ccttcggcaa gcatccttca gcgggcatcc
    31861 ttcagcggga aagcagtaaa ttccatcgaa tacatcaaaa ccccggttaa gccagccggg
    31921 tatatctccc aaaggaactc attggattaa aaataaatat gtatttttgt gcgttacaaa
    31981 aaaaaggcaa tgtacaggaa acagatactc atttttatat tctgcttgct atgttcgcgg
    32041 gcactttggg cagtgatggc ttatccctac cctgtagaag tcgatttgcc cgatggcagt
    32101 aagttggaaa tacaacgtca tggagatgag ttttttaatt acactaccga ccaaaacggt
    32161 tatattatag ctcaaaaacc ggacggttat tactactacg ccaactacga cgaacaagcc
    32221 aaactcaata tcagcaacta ccgtgccggc agccgggcgg caaggcgttc ggccgtggtg
    32281 cctgaagtca atttcaggcg ggcggctcag acccgaagcg agcgttacca agcaagccgg
    32341 gatttaatca gggccaacgc taagcaagct tacgagggca ggctaaaaac gctcgtttta
    32401 ctggtggctt ttcaggacaa ggcttttgtt tctccgacgg cgcacgatga tttctacaga
    32461 ctaagtaacg aattgggtta cagcgataac ggtgcaaccg gatcggtaag agattattat
    32521 cgggacaatt caaacggagt ttttgacccg gaatttgttg ttatggggcc ctataccctg
    32581 tcaaaaccca tgagttatta cgggggcaat aacgaggaag gtaacgacaa cgatcccagg
    32641 cgcatggtga tagaagcagc ctggatggcc aacgaggaag gtgtggattt ctctgagttt
    32701 gaccagaaca acgacggcta tgttgatttc atctatgtgt attatgccgg atacaacgaa
    32761 gccgaaggcg gtcctgccgc cagtatatgg ccgcattcct ggtcggttgc ctatatggac
    32821 agtacactat tggacggagt aaaattgggc tattacgctt gttcttcaga attagcaggc
    32881 gcatcgggtt cgacgatgtc cgccattgga accgtttgtc atgaatacgg acataccctg
    32941 ggtttgatgg atgtgtacga taccgatgga gcagaaagtg gaggaatgtc gggaggcttg
    33001 ggtgcattga gtttgatgag ttcgggaaat tacaacaata agagtcgtac tcctccgcat
    33061 ctgacttcgg aagaacgctg gtctttgggc tggatgaccc ctacaaccat aaccgaagcc
    33121 ggagactatt cgttggcttc catagatcag aatcaggctt atataatacc tactttaaac
    33181 gaaaacgagt attttcttct tgaaaaccgg cagcataata attgggatct ttacatcaaa
    33241 ggcgagggtc tgttgattta ccatgtggat aaatcgagaa acatggtgct tggtaaaaca
    33301 gctttggaga gatggtatac caacaccgtc aatgtatatc cccaacatca atgtatggat
    33361 atggaagaag ccgtgggctc cgaatccaag ggtgacatgt atgccttcta ccccggtcct
    33421 tcgggcttgt atactgcttt tacccgtacc accactccgg cagctttagc ctggtcggga
    33481 ttaccattgg gcaaagaact ttacgatata agattggaag gtcaagaaat tcacttcaga
    33541 gtggaactcg aagaccaggt ctatttcgga gggcaggtaa tcaacagaga aggccaacct
    33601 gttcagggag cacttctcta tcttaaagag ctcgttgagc agccggtgtc gggcaatacg
    33661 accggtttac gttccgccat gcatatttca ggtgtttatt ataccactac gagcggcatc
    33721 gacgggcgtt ttatttttga cgaagactta gatccgggcg tgtatgaact tttatgtcag
    33781 tcggaggcat atatcagtaa ttctcagatt gttgaattaa aaccaggcag taacagcgtc
    33841 ttgattactt tgcttaccga ggccgaacaa ttgatagaag gaactttaag ttggtgccgg
    33901 gatttcagtt acggaggagc tatcggaaac gaaggggaat ctttcgtggc gggtgcctcc
    33961 tggacggccg aagagctgag tcagtaccgg ggaatgtata tagggagaat gacctttttc
    34021 attgccggtg gtaatcccga tgttgaggtt agcatctatg ccgacaggaa tccgatattt
    34081 aagaaaaaca ttgctgcaga agacttgact gccaatagtt tttacagcct gaatttaatg
    34141 gccgacagtt tggtcataaa ccccgaaaca gaattaattc ttgcttacca ggttagtaat
    34201 taccccaaaa acgcttatcc ggccggaatt tgcggtggaa gtagagtaga gggtaaggga
    34261 aatttaattt cactggatca aggagctacg tggacaacac tatatcagga agcagaaatt
    34321 gaaggcaatt ggttgatttc tttcgatata tatcgtggtt cggattttgt cgcaatggaa
    34381 tctttcaacc tggatcccac agatttgacc atgaaaatcg gagaaacccg gtatttatac
    34441 ggtcaaataa aacctgcgaa tgccacaaac ggaaaaataa gctggtcgag ttcggattct
    34501 tccgtggttt gggtggatca aacgggtaaa gtctttgctc ttgcggccgg tcaatcggtc
    34561 gtaacggcaa caacagacgg aggaaatcgt tctgccactt gccaggtaaa agttgttccc
    34621 accattgcct ccagtctaaa cgttaacgta gcgcaacatg tgttttttgt ggattgggac
    34681 gaggcgaccg gagatgcctg gactttttcc tggaggaatc ttggcgattc cgtatatcaa
    34741 gataccacac ttactcaaag ttcttttcta ttggaaggcc tcgaccccaa aagcacaacg
    34801 gaggttttgg tgcaggctta ctccgaacag caattggtgg atgaacacgc ctttagcgtt
    34861 actacggcca acaaaaaatc ggatttcatg gccatctata caacggaaac tttctctccc
    34921 ggacaaaaac tattgctcga tgtgttgaac ttgccggaag acgcctctgt aaatgtgtgg
    34981 aaactcaacg acgaagtcgt ttcgggctct attctcgagt tgtccgaagg catacatgaa
    35041 ctaagggtag aaataactac ctctaaaggc atagaaataa tacgccggca attaaaagtt
    35101 gaataatatg agaaggcatt ttagtatact ggcacttatc ctgttgaccg gcttgcttta
    35161 cctggcttgc gtaagggaag accctttgtt gaaggctgcc aaggaacatc ttgtaagcca
    35221 ggaaatctat ggtatgtaca aagaccggca agccgttttt atttacgatg ccgataaaca
    35281 tcaattggcc gacaatgcgg aagctcttta tttccgaatc caaaacgaca gtcaaaatac
    35341 gatactgcaa atgactctcg atcaggagcc ggatttagga aaaaaatgcc tgatgactat
    35401 tgatgctaaa ggtcttagcg gaatagaaac ttcttattcg gtagaggttg taaaaatgga
    35461 cgaaagcaaa caaaccctgt ggttgttcga tcagaccggc aaaacaggct ttgttatgtt
    35521 ttacaggttt taaaacttag atgaggatct tccgcgtatt ttgtttgctt tgtatttgta
    35581 gcttcggatt cggcgcagag aaatcaatac agcttagagc ggatgaatat ccgggaacaa
    35641 gcctggctta cgtggttcag gaagttcagt cgggcaagct gttggccgaa atgaattccg
    35701 cacaggcttt ggttcccgct tcgctgagca aactgttgac tacgggcgct gcgctggaaa
    35761 tattggggcc cgaaaagcgt ttcaaaacgg agctggccta cagaggatta ttggaaaagg
    35821 gcagcctcaa agggaacctt tatatactgg gtggaggcga cccgtcgctg ggatcggaat
    35881 acaccgaaga acaagccgga ctttttcttc aagactgggc tcaggcggtg aaagagttgg
    35941 gaataagccg tattgagggc gatatcgttg ccgacccctc catttttgac caagaagccg
    36001 tctcgcctta ctggctatgg gaagatatgg gcaattatta cgcggccgga gtgtacggcc
    36061 tggcggtctt tgataatatg ttccgtctcg gactgaaatc gggcttgccc ggtactaaac
    36121 ccgagattct ctatcttaaa ccggcattgc ccgagatgcg gataatcaat aatctaattg
    36181 ccaaggacaa tgccaaagac agtgcttatt tttacggggt tccctatcgt tgggagcggg
    36241 tattgtacgg cagcctgccg gccggaagag aagaattcgt catcaagggc gatattcccg
    36301 atcctcccgc ttacctggcc tttaggttgc aacaagaatt gatcaaggcg ggaatcgacg
    36361 tgcaaggtag agcccttagc aagcaagaac ccggctctat ctgcaaggaa cccggctttt
    36421 tcccgaagga atacgttaat tccgcaaata atccacaggc tgaccatgag gccgtcgttt
    36481 tttataccgg ctattccaaa cccctgggtg aactggtgaa aataatcaat taccgcagcc
    36541 ataatgtgta cacggaatac ctgctgcgac atttggcgct ggctgccgga gcaaaggctc
    36601 cggtctctgc ccgggaggga ctggaagcct tacagcgatt ttggtcgggt aaaggcgtgg
    36661 atatcagtgc ctggcaattg tacgatgctt cgggtttgtc tcccctgaac aggctggatg
    36721 cggcaagtct cgcccaagtg ttacgctata tggccctcga aagcccccgg gccggtctgt
    36781 tttccggcag tctgcctcag gccgccttgg aaggcacggt ggccgacttt gggaaaacct
    36841 tgccgggaac actacgcatg aaaagcggat cgatgaaatc ggtaacgaat tacgccggtt
    36901 attggcggct gggagataaa acctatgtgg tggttctttt atgcaatcag cacaaacaat
    36961 ctgccgcccg catacgcaga gatatggagc aactattaag aaccatttac aataagggat
    37021 taaaatgaat aaggaagaac tttttaagga actgaaagaa ttaaatttat tgtggtcggg
    37081 tttgagccgg attcccaact ttcccaagga attgctggag ctttcgctga acaaactcga
    37141 aaacatcaac cgtctggtaa aggaccttga ggagacgagt tatcagccac aggtaaaacc
    37201 cgcttttgag aaggaaacgc atacccggga agctactccc gccccccttg cagccgattt
    37261 gaaatcaaag ccctcagtag cagaggaaac caagccgata cccgctcccc agagttcgac
    37321 cccgactccc gcaagctcat cccccgcgca gtcaagcccg ctcacgacat ctgtaagtcc
    37381 gctatcaaca cgggaaagat ctctcccaac gcaggaaagt tcgctccccg taaaggaaag
    37441 cgggcagacc gaagagcctg tttcgccccc ggtcgggaag atgcccaaac tgcaaaccga
    37501 aaaacccgaa agcctggtgg atcgttatga aaaaaccagt tccaaagaat ccttgcaaaa
    37561 gaaattggaa agcagacatt tcgccgattt atccaaagcc ctgagcctaa acgatcgttt
    37621 ccgtttcagg cgcgacctct ttggggattc tcccgagact atggaacagg tattgcaaga
    37681 cattaatacc atgcaaagta tggatgaggc gctctcttat ctacacaaaa attattctca
    37741 acacagtgaa acggagagtt ttggcgattt ttgcaagcta ctgaacaatc atttctccag
    37801 ctaagaagca cagcagccat gtcaaaactc agcatagtgc ccacgcccct gggaaacctg
    37861 gaagatatta cgcttagagc gctaagaata cttaaggaag ccgatttgat tctggccgaa
    37921 gacacccgta ccacttccaa actactgaaa cattacgaca tcggcacgcc catgcagtcg
    37981 catcataaat ttaacgagca taaaaccgtt gagcaggtgg tggcgcgcat acaggccgga
    38041 gaacacattg ctttggtctc cgacgccggc acacccggaa tttccgatcc gggttttttg
    38101 ttggtgaggc aatgcttgca agccggcctg caggtagaat gcctgccggg agccactgct
    38161 tttgtacctg ctctggtggt atcgggtttg cccgaggaac gttttcattt cgagggcttt
    38221 ttgccgcaga aaaaaggccg ccttacccgt ttacgctatt tggcggaact gacgcatact
    38281 tttgtgttgt atgaatctcc ctacagagtg ctcaaaacat tgacgcaact ggccgaaata
    38341 tgcggcccgg agcgcagggc ttccgtcagc cgtgaaatca gcaaaatcta tgaagaaacc
    38401 ttgcggggca gcctggatga acttatccgt cacttcagcc tgacagaagc ccggggggaa
    38461 tttgtgttgg tcgtggcggg aaaaaacgaa tcccgggagc agtcttcttt ggagaaagac
    38521 cacccccggg aataaactta tttttcggaa atattaatct ttcgagaaaa gtttggtcga
    38581 agatatcttc accactttcc cgtatgcgcc cagagctttc aggtcgatga gcttgggatc
    38641 tcccacgatg gctatggcca tgggtttacc tttcagttcc tgttgataga attccaacat
    38701 ttgttgcagg ttgaattgtt gaatttccgc ttcctgctgt aaagcgggat tggccgtata
    38761 acccaggcgt ttccattctt cgtattgctg acttttactt ctgaagtcgg gttgggtggt
    38821 aaaaggttcc aattccaggt aagctttcaa attcttttct ctttccccat acaagggcat
    38881 gtcgtcgacc aatttcatat acagggcaac ggcatcgttt accttgtccg attgtgttcc
    38941 aacataacct tggaaacaaa cttgcttttc ggctagaggg ggagttgcta aaaatccgta
    39001 ggcagtgtag gccatggaat ttttttcccg aatttcttca atgatcaaac tggtcagccc
    39061 tcccgctccg aaatagcgat tgaaggcttt atatttaagg tcgttttcgg gtttgtaaac
    39121 attgccggga gcgaagaaat atattttagc ttgtttggtg tcgctacgag gtagaaagta
    39181 aatggtgttt tccgaatatt ccttgtactc tttccattcg ggagagctgc tttctttctc
    39241 gttggctttt agaggcaagt attgactcaa gatatcgtaa gcttcgtcaa aaggcattga
    39301 accgcaaaaa tgcacttctg cctcgtagtc ggttgcccgt tggaattcac cggtaagttt
    39361 acttagtccc atttcggtga atatattgta caatgtgggt ctgtcaagat aggcggattt
    39421 gtcgccataa accatatatt cagccagggc ttcggcaatg atctgaggat ctttgccttc
    39481 acgtatacgg ctttggtaga tacttccctg gacgttgcga tattgttttt cgtccagttg
    39541 aggcatcaga atctgacggg tcagtaaacc aagtgcttcg accaggtgct cttcgaagcc
    39601 ttccattacc acatagagat aatcttcgtc gacgctgtaa cgacaggtga catttagttc
    39661 ggtaatggct tttttgaatt cctggggatc atacccgccc attatacccg cataattcat
    39721 cagggtggta gagtattcga ggtaaggcat tttctcggta ccgattccgt atttcagaat
    39781 caaagagaaa acctcatttt cggtgttttg ggtataaaac agtttggacc tggtattgat
    39841 tggtttgatg ataatttctt cgaaatcttt tacaggcagg ctttgttttt cgtagctcag
    39901 atcttcaaaa gccagggcat aagccgattt cttttcggaa ctctgagtta taggcttgat
    39961 gtcgggtttt tccaattttt ctgctgccgg aaattttccg gtaccgatat cgaagagcat
    40021 ataattgttg ttacaatagt cggaagctac tctttgaata tcagttattt gaatcttatt
    40081 aagtcttcct gtataatcca atgcctcgtc cagtgccttg ccggaaataa aggccctggt
    40141 caggatttcg gctttgtcgc tgttgctttc caggttgagt tggtagctgc ggatcatagc
    40201 ctttttgatg ttttccaaga gcttggcatc aatgttgcct tgcttgatgc gttcaatttc
    40261 ctggaaaaga agtttttcta ctgctttggt gctggcaaaa acttgttggg cgggatcgta
    40321 cgaaggaatg gcggagatta cgatacgtcc ctggtcttcg aagctgatat ggctggcgga
    40381 agctcccatc aagtccccgt cgagcaccag tttgtcgagg tagccggttc tactccggtt
    40441 cgaaagtaaa ttcgtaaaca gatcgattac cagtgcatcg ggatttgtta ttccggcgcc
    40501 tttaaaacca acgtgaatgc ggggaatata agccaggttg gctttaaaat ctttccggcc
    40561 ttctactttc cagtcggaaa attcaggcag cgaggggagg gttttggcgg gcagggctcc
    40621 aaagcttttt tcgatcaggg gccgggcggc ttgagggtcg atgtttccta ccagaatcag
    40681 cgccatattt tcgggcacat accaattgtt gtaaaattca atcagtttgc ttatttgcgg
    40741 atttttgagg tgttccgaca gaccgatgat cggcctttcg tagggatgtc ccttaaaaag
    40801 attattcaga agataatgaa attctctttg ctcaagttga tcttcgtaaa gattgtattc
    40861 ttcgtaaacg gtttccagtt ccgactgaaa agttctgaaa acaggagccc ggaagcgttc
    40921 cgcatagagc atcaaaaaac gttccaattg gttgggtgga aaatcgttgt aataagttgt
    40981 taagtcgtaa gatgttcctg cgttcatacc ggttccaccc atggtggcga ccagggatga
    41041 aaactcattc gactggctaa attcggaagt tttaatactc aattcattga tttccttatt
    41101 caatgcttct tttgttttcg ggtcgctgct ggcagctttt tcgtcgtatt tggcgatgat
    41161 ttgttcatag agtatttttt cttgttccca gtcggtcgta ccaattcggt cggttccctt
    41221 aaacatcagg tgttccagat agtgagccag gccggtaaat tgttcggggt cgtttttgct
    41281 gcccacgtgc acgcttaccc tgccgtgtac gtcggtctgg ttgctgtcgg gccagatata
    41341 cacctgcata ccgttgccca gcttaaaatg cgatacagag ccgaccgggc cgttttgagc
    41401 aacagcggta acggcaaaag tcagaaaaaa acctgcggtg aaaaaacgca ggaatgaacg
    41461 taaattagat tttttcatat gcttgttgtt agaataagtc ataaaggttt ttagttttta
    41521 gttttgtaat aatgctgcat aataatactg caaaggaaac actttttttt gtttttgctc
    41581 tcacttactc ctatctttgt gcttcaaaat tgatgcatgc agccgaattt cacttttgat
    41641 accttttccg aaagaagcgt tttggtgggt ttaattaccc ctgaacaaaa cgagcagaaa
    41701 gctaccgaat atttggatga attggctttt ctggcccaaa cggctggagc aacacctgtt
    41761 aagagatttc tgcagcgatt ggatcagcct aatcccaaaa ccctggtagg atcggggaaa
    41821 ctattggaga tcaaggagta tgtcgaaaac aacgaaatta ccatggttat ttttgacgat
    41881 gaccttagtg ccaaacaact taaaaatctg gatcaggtac tcaaagtgaa aattttggac
    41941 aggaccagcc tgattctaac tatttttgct gaacgtgccc agactgctta tgccaagacg
    42001 caggtagaat tggctcaata tcagtatttt ttacctcgtc tcacccgctt atggacccac
    42061 ctggaacgcc agcgcggagg aggtggagta aagctgcgcg gtcccggtga aacccaaatc
    42121 gagacggacc ggagaattat cctaaataaa attgcgcagt taaaaaaaga gcttaaacaa
    42181 atcgataaac aaaaagtagt acaacgcagc aaccggggta aaatggtccg tgtagccctg
    42241 gtcggttata ccaatgtggg caagtcaact ttgatgaacc tgttgagtaa aaccgagtta
    42301 ttagtcgaaa ataaattgtt tgccaccctc gacaccaccg tgcgcaaggt tattatcgat
    42361 aatcttccct ttttgttgag cgataccgta ggttttatcc gaaaactgcc tcacgatttg
    42421 gtggaatctt ttaaatcaac cctggacgaa gtccgcgaag cggatttgct gattcatgtg
    42481 gtagacattt cgcatcccgg cttcgaagaa cagattctgg tagtagaaaa cacattgacg
    42541 gaattgggtg tgcacaacaa accggtcatt ttggtattca acaaaatcga tgcctactct
    42601 tttgttccta aagaagagag cgatttaagt ccggaactgc cagaaaacag gactctagag
    42661 gcattgcaag cctcctggat gtccaaaatg caggataatt gcctctttgt ttccgccgtg
    42721 gagcgtacga atattgataa tctgaaacaa ctgctctacg ataaagtaaa agaaattcat
    42781 gttacccgtt ttccctataa cgatttttta tatcctcttt acgatgtctc cgaaaaagac
    42841 agttcacttt aacatttttc cgttatgata ccaccttatc gcaaactaac tccatccgaa
    42901 attgaacaac tcggttctca ggcctgtacg gcagatgatt gggataaagt tttagtacac
    42961 gaggattttt cggccgaagc catacgcagt gtacatttct cgggcgatat taagctggga
    43021 aagtttgaag cttgccatga cctgcccgga aacgtaaaaa agaaatccgg tattcgccgg
    43081 gctcacctgc acaactgcga ggtaggcaat aatgtactta tcgaaaacat cggagaatac
    43141 atcgccaact acgtcatcga agataattgc tgcatcgaac agctcacctt tatgatttgt
    43201 gatgccgaaa attctttcgg caacggagta gaaatcgaag tactggacga aaccggaggc
    43261 cgtgtatcgc gaatgtacaa cggcatctcg gctcacactg cttatctgat ggctatgtat
    43321 cccggacgca atgatttgca ggcacaactc aaaaagttga ccgagcacaa agctatgcaa
    43381 gagcgttctg ccatgggcag ggtagggcat tcatcttacc tgatcaatgc cggtatgata
    43441 ttgaacgtca atatggggcc ttttaccaaa gtcagaggtt cttcnnnnnn nnnnnnnnnn
    43501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43561 nnnnnnnnnn nnnnnnnnnn nnnnccggta tgatattgaa cgtcaatatg gggcctttta
    43621 ccaaagtcag aggttcttct tttttgtaca acggcagcat caacagcaca gaagaagctc
    43681 cggtacaggt tggggccgaa gtcaacgccc aaagtttcat attctcgccg ggttcggaaa
    43741 tcaaagacgg ggtgcatttg gtacgttgtt ttgtgggaga ggcctctatt ctcggacacg
    43801 gtttcaccgg tcatgattcc tttttctttg ccaactgccg catggaaaac ggagaagcct
    43861 gcgccagttt cgcaggccct ttcagcactt cgatgcacaa atctaccctg atgattggag
    43921 gtttgttttc tttttgcaac ttcggctcag gctccaacca aagtaaccat ctctacaaat
    43981 tgggccccat gcacagcggt gtactggaaa gaggtactaa aacaggcagt gattcttatt
    44041 tattgtggcc ggctaaaatc ggtcctttct cgctggtcaa gggccgccat gtcaacaacc
    44101 ccgatactac ggctttcccc ttttcttacc tggtcgaaaa cagaggtatg agttacctga
    44161 ttccggctgc ctccctgccc agcatcggat tgttgcgcga cgtgaacaag tggcccgacc
    44221 gtgatttacg taaatcgtcc aaaaaacaag acaagattat tttccatttt ctcaatccca
    44281 ataccgtaga ggaaatccga aaaggcatta gaatcggtaa tgacctgcgc ttacgcgctt
    44341 ctctgggtta taaccccgaa gacgccgcca tgcagggcgt ggtaagcgaa ctcagacaca
    44401 ctgctcccga cgatcccatt ctggaatacc gctaccgggg aatgcgcatc cgtcgttcca
    44461 atctcgaaaa aggttctgcc ttgtacaaaa tggccataca aatttacgtc ggagatgttg
    44521 tgttacggca aatagaagct ttcaaacaga aaaaagaccg ggaagacgac aaaatcatct
    44581 ggaaagactt tatcaaaacc cgcgtgttcg aaaaggaaat tccgtataat caggaaaagt
    44641 ggctggatat agcggggctg gttgctcctg aggcggccgt agagtacctg atcggttgca
    44701 tcgaaagagg caaatttacc agtgtagaac aactcgaaga tcgttgggaa gaattgtatc
    44761 agacctacca ggaaagggag tgggcttatg cctttccact gattgccgaa ctgaggaaca
    44821 tcgccttgcg cgacatcaag cccgccacct tgcttgctat cctggacgaa tggcaggaag
    44881 cctcgcttac gctttgcaat caggcattgc tcgatgctga actcgaattg tccatgaaac
    44941 gatcttttgc tcccgaagaa ggtgaagttc ccaattcctt tgttgagaaa ttacgcaaat
    45001 ccattggcga aatacccgga aaaggcgatg tcgccaggca atttattcag gataatattg
    45061 taatgcagaa taacctgcat taattgttta ctgtaaatac ccttatactt ttcttatgtc
    45121 aacacccgag tttaagtatc aaaatccttt tcccctggga aaagacgaca ccgaatatta
    45181 tttattaagc aaagactata tttcgacggc tcaattcgaa ggcagagaaa ttctgaaagt
    45241 tcaacccgaa gctttgagat tattggcaag aaccgctatg cgtgattgtt cttttttgct
    45301 caggccggcc catcaggcgc aggtggctgc catattggac gatccccagg ctagtgaaaa
    45361 tgataaatat gtggctctca ccatgttgag aaatgccgaa gtttcggcca aaggcgtgtt
    45421 gcctttttgc caggataccg gcaccgctac tgtggtagcc aaaaaaggtc aggaagtctg
    45481 gacgggcggt ggagacgaag aagcactttc gcatggagtt tacgacactt ataccagcga
    45541 aaacctgcgc tactcgcaaa ccatacccct cgacatgtac cgcgaaaaaa attcaggtaa
    45601 caatttgcct gctcaaatcg atattcagtc ggtcgatggc aacgaatacc actttttgtt
    45661 tatagctaag ggcggaggtt cgcaaaataa gacctatttg tttcaggaaa ccaaagcctt
    45721 gttgaatccc aaaacccttg aggctttttt gatcgacaag atgcgcaacc tgggtaccgc
    45781 cgcctgtccc ccgtatcata ttgcttttgt tatcggaggc agttcggccg acatgacact
    45841 taaaaccgtc aagttggcct ctacgcatta ttacgattcc ttacccctgg aaggtaacga
    45901 ctacggacaa gcgttccgcg acgtgaaact ggaggaaaaa atgctgaaag ctgccggaga
    45961 actgggtttg ggagcccagt ttggaggaaa gtattttgct cacgatattc gcattatccg
    46021 cttgccccgt cacggcgcct cttgtcccgt agggttggcg gtctcctgtt ctgccgaccg
    46081 caacattaag gctaagataa acaaagaggg catttggctc gaaaaactcg aagaaaaccc
    46141 cggcaaatac atacccgaag ctttacgccg gtcgggcgaa ggtgacgctg ttaagataga
    46201 cttaaaccgt cctatgaaag aaattttggc cgacttgagt cgatacccgg tggccacccg
    46261 tttatccttg aatggaacca tcatcgtagg cagagacatg gcccacgcca aactcaaaga
    46321 acgcttggac gaaggaaaag gtttgcccca atatattaaa gaccacccca tttattatgc
    46381 cggcccggct aaacagcctc aaggcctgcc ctcgggttcc tttgggccca caacagccgg
    46441 acgtatggat tcttatgtgg aattgttcca gcgaaacggc ggcagtatgg tcatgatagc
    46501 caaaggtaac cgcagccggc aggtgaccga ggcctgtcaa aaatacggag gtttttatct
    46561 gggcagtatc ggagggcctg ctgctatttt ggccgagcaa aacatcaaat cggtggaatg
    46621 cctggaatat cccgaattgg gcatggaagc catctggaaa atcgaagtgg agaactttcc
    46681 tgcttttatt ttggtcgacg acaaaggcaa cgatttcttt gagcaaatca agccggcttg
    46741 tttaaactgc tgagaatcac tccctgtatt ttccatcccc ggcatcttcg atgatactca
    46801 ggtagcttag ataccgcgat tggctgatcc ggtgttcttc gatcgctttc agcacggcgc
    46861 aatccggctc gtgccggtgt gtgcagtcgt tgtagtaaca acgggccgat gtgcgaaaga
    46921 tttcgggaaa gaaatgacct acttcggcaa catgcatatc taccgtatga aaccctttaa
    46981 ttcccggagt atcaataatg taagtatcct cttcgatttc gaacatttcg gaataagagg
    47041 taacatgcct ccctctgttg tggtaatccg atatcggacc aaccttggtc tgaatggccg
    47101 gattaagtct gttgaccaaa gatgacttac ctacaccgga gttgccggcc agaaggcaga
    47161 ttttacctct tatcagcgag cgcacttcct ctatgccttc gcctttgaga gcggatactt
    47221 tcaggcaggg ataggggatg gattcgtaga gcctaatcag cgcttcgagg tattcggaat
    47281 cttcagcccc gtagaggtct attttgttga aaagcaggca gcagggaatg cgataggctt
    47341 cggccgtagc cagaaagcgg tctacaaaaa tggtatgagt ttgagggtgg tttacggtga
    47401 ccatcaatat ggccaggtcg atattagccg ccagaatatg caattgtttg gaaagattgg
    47461 ccgctttgcg tactatgaaa ttgctgcgat ccagcacctc agtaataaag tatgtctgtt
    47521 cgtcggaggt tttttcgatc agcacacgat cgccgatggc tacgggattg gtgctgctca
    47581 ggtcttttat cctgaaattc cctttaacca cacattcgtg caagtccccg gcaagagttt
    47641 tgaccaaaaa gacattgccg gcggttttga tgaccagtcc ttccatcgac gaattaaaca
    47701 gtcatgattt ccttttcttt gagggccact atctcatcga tggatttgat gaatttgtcg
    47761 tgcgttttct ggacttcggc ttcggcattt ttgcctatat cttccgacag gccctctttg
    47821 accaatttct tgatggattc tatcgtgtcg cggcgggcgt tgcgaatgct gattttagct
    47881 tcttctcctt cgtttttcac ctgcctgctc agctgacggc gcctctcttc ggtgagcgga
    47941 ggcaccgcca ggcggatgat ttcgccgttg ttaaccgggg tgatgcctac atcggaagcc
    48001 ataatggctc tttcgatttc cttgatcata tttttttccc aaggttgtat ggcgatggtc
    48061 ttgggatccg gagtactcag gttggccact ttgtccaaat ccgtctgcgt accgtaataa
    48121 tcgacgcgga tgttatccag aatactgaca ttggccttgc cggcacgaat ccgtgaaagg
    48181 gtttttgtca gaaagtcgat ggtttgagac atattctctt cggtcatgct caggtaaaga
    48241 tccaattctt cgttcatagg tatagtctta aagtgtttgc tgaaattaat tgtgaaccag
    48301 ggtgccgata ttttctccct taaggacttt taccagattt ccttttttgt ccatgttaaa
    48361 gacaacgaca ggtaaattgt tttcccggca aagagtggta gcagttaagt ccattatttt
    48421 gaggttcttg tcgtagattt cttcaaagct gatcttgtcg tatttaacag ctttggggtc
    48481 tttttcggga tcggccgtat atacgccgtc cacgcgggtg cctttaagca tcagatcggc
    48541 ctttatttct acggcacgca gcgacgccgc agtgtcggtg gtgaaaaacg ggcaacccgt
    48601 gccggccgaa aaaatgacca catggccttt ttcgagaaaa tcaatggctt tatcggtgct
    48661 gtagtgttcc gctaccggct ccatattgac ggccgtaagc acgtggttgg atacctccag
    48721 tttattcaaa gccgaactca gggccaggct gtttatgaca gtggccaaca tacccatctg
    48781 atcgcctttg acgcggtcga atccgctctg ggaaccgctc aaacccctga aaatatttcc
    48841 accgcctatc actatgccca cttgtatgcc catgatctgc agctctttga tctgttcggc
    48901 atattcgttg aggcgcccgg gatctatccc gtagttgctt ttccccatca gcgactctcc
    48961 actgagtttc aataatattc ttttgtattt catgctgatc ttgtcctgga cgcacaaaag
    49021 tacatttttt ttgcgctaaa tcaaatactc tatttcttta aatgcgaagg ctctgatcca
    49081 tccctcgtcg ctttcaactt ccaactgccc gcttggcatc acccgaagta ttctggccct
    49141 gaacggtctg ttcccctgtt tatagtggtg aaaaccttct ttgcggtaaa gatgtcggtg
    49201 gtaagctgct gcaatttctg tttttccttc ttccgtgaag gttttggcat aagcctcacc
    49261 cagccggcag tgcagtgcaa gcgttaaatc gactaagtca aaagtccgcc cgcacaactg
    49321 aaagacggaa acaggattgg gcaggtttgg gctgaattgc t
//
LOCUS       DAOSTD010000002        45272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_930_length_45272_cov_15.035341, whole genome shotgun sequence.
ACCESSION   DAOSTD010000002 DAOSTD010000000
VERSION     DAOSTD010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 45272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45272
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_930_length_45272_cov_15.035341"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="PLI37_00175"
     CDS             complement(<1..1277)
                     /locus_tag="PLI37_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ57465.1"
                     /translation="MSLPKFTRAFWVANVVELLERLAYYAVFIVITLYLSNIWGFSDI
                     EAGLISGVFSASLYFLPTFVGAYADRIGFRQSIILAFSLLSLGYFGLGLLPTLLESAG
                     LVSYGDTTVFSGLPESPLRWSIVPVMILIVVGGAFVKAVISGTVARETTSENRARGYA
                     IFYMMVNIGAFTGKSVVDPLRKSLGDMGLVYLNYFAAAMTLLALIAVFFLYKSSQTQG
                     EGKSLREIWRGLVKVFTNARLIVLILIISGFWMIQSQMYATMPKYVIRLIGESASPGW
                     YANVNPLVVFLLVNFITSLMAKKTALTSMTIGMAIIPLSALVMSLGHQLGSTPVLGMH
                     PVALLMVIGIAMQALAECFISPRFLEYFSLQAPKGEEGLYLGFSHLHSFISYLFAFGL
                     SGFLLDKYCPDPRLFDSAEAYAQATVNAHYIWYV"
     gene            complement(1287..2018)
                     /locus_tag="PLI37_00180"
     CDS             complement(1287..2018)
                     /locus_tag="PLI37_00180"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HOQ57466.1"
                     /translation="MIAFSPKRPVFLFYLWVVVFLLIQSPVPAENKQSGQNKTATFAV
                     EEEQVESTSAENPGRQAPDGLPGKRVAVKLNGLLLAGVVNPAVEFAVHNNISVQLEGM
                     GVFYHDHVPFLNFPLVLGLSFIEGRFYPKEVYRGFYAGPNLGWGVWKMNKSIAPGYFG
                     SYNNNSVQYGHNVMLGLNLGYQWVLSKRWSLDLNLGLGWQHSTYEGYKNGVLYTDPSI
                     PEGRPNRSAEWMPAYKGGLFFSYRF"
     gene            complement(2028..3566)
                     /locus_tag="PLI37_00185"
     CDS             complement(2028..3566)
                     /locus_tag="PLI37_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ57467.1"
                     /translation="MKRIFSLLVLCLLAANTWSQDQADANTESQDVTGSWYGRISVMG
                     QELRLNFHISNEAGQYSATMDSPDQKAFGIPMQSATYEDQVLILKVSNITYTAKLDSA
                     GNLNGEFKQAAIKTGLYMTHQPLTDSTEQKVRRPQEPRPPFPYQSEEVRFKGGGDYQL
                     AGTLFKPRKGNNPPVAVFISGSGAQNRDQELLDHKPFLVVADYLARKGIASLRYDDRG
                     TAESGGDQYEGSSYENALDAAAAVDFLKASGYRQIGLIGHSEGGLIAPMVASIDSGIA
                     FIVSLAGPGVTGAEIIKKQSFDIAKAEGTAEDTLEVQKILAEYIFDRIAALPENEPDS
                     IAGIIEGALQQLNPEQSEIALGGMSREEYIQASVQQLGEEWMFHFLGFDPAQAWKEVS
                     CPVLALNGEKDLQVNADINLAAIQQALTEGKNPKLTIIKYPNLNHLFQNANTGSPSEY
                     ANIEETFSSKVLKDISKFIRKSTGMHQRTKLFIDGGIAALLLILLFIVLKKTSKKTTD
                     SPNV"
     gene            3681..>4210
                     /locus_tag="PLI37_00190"
     CDS             3681..>4210
                     /locus_tag="PLI37_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOQ57468.1"
                     /translation="MKKLSILEMNRLSAEDFKTAPKLPLTLVLDNVRSLHNIGSVFRT
                     SDAFLVEKVILCGISSCPPQPEIHKTALGAEFTVDWMYEEDCLQAIKALREQSYIIYA
                     AEQTTGSICLQDLRLDPGQKYALVLGNEVKGVQQEVIDLCHHCVEIPQYGTKHSLNVS
                     VASGILVWSFFEQLRKR"
     assembly_gap    4211..4310
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4311..5029
                     /locus_tag="PLI37_00195"
     CDS             <4311..5029
                     /locus_tag="PLI37_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOQ57469.1"
                     /translation="LGKPRFLSDEKINEFHARIPHNISHFMDVSYPSDERFIRSLMCN
                     IIRRSCDQGLMISTYGTVSVRWRNDDFLITSPNVPRWDVQPEDIVQVRNGMAEAGKTP
                     SRSVALHHRIYQKNPQINSIITTQPPNLMAFAVSGTKFDVRTIPESWIFLQDVPSIPF
                     GMHYEEIDRVADMLGRNRAILVDNDCVIVTGDKLLQTFDYLEVAEFSAHSLVMASAIG
                     PLQPIGEEEIEELRKAFNVK"
     gene            5046..5882
                     /locus_tag="PLI37_00200"
     CDS             5046..5882
                     /locus_tag="PLI37_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HOQ57470.1"
                     /translation="MRIIRSKRNDTAWHLAAEEVLFQGKDAVLLLYINPPSVVIGCNQ
                     IMENESDTAYCRENNIGIYRRISGGGAVYHDEGNINYCFIQDKNPQQSSLNGAFLEPV
                     EQILQSLGLQVSRARRKDLWAGGYKISGTASHIGKGRELHHGTLLYDSNLEALHAALG
                     LIPGEKAEQSPGKKQQPGSRGIVSVPSPVANIRSLYPSFAALSTQEFFAAFFEAAHCY
                     YGQSVYGQSVWDGEEQGLKEGQKKPKEDKERLQGFREKELIEIEALCRQKYRNKAWNE
                     LK"
     gene            6013..7674
                     /locus_tag="PLI37_00205"
     CDS             6013..7674
                     /locus_tag="PLI37_00205"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOQ57471.1"
                     /translation="MLSLVAAVLIASLQAVYPCTNLIVTKGASADGSVMVTYSADSHQ
                     LYGELYFRPAADYPAGSMLQIYEWDSGKYLGEIPQAEHTYSVTGNMNEHQVVIGETTF
                     GGRPEFYDSTGIMDYGSLMFVALQRAKTAREAIAVIDRLMQDYGYASSGESFSIADPD
                     EVWIMEIMCKAPRYNKKGKNLNKGAVWVAKRIPDGHISGHANQARITNIDFNNPDDCL
                     FSKDLISHAREQGLFTGKDEEFSFCDVYAPADFGALRYCEARVWAYFNRFAPGMDKYL
                     NYAMGYDLNNKMPLSVEPTRKLTVKEVADMMRDHYEGTPMDMTLDIGAGGGACPYRWR
                     PMEFKVDSLTYLNERAIATQQTGWWYVAQCRGWLPRDLGGLFWFGVDDAGTSPLMPIY
                     SASLEVPECVREHNGSMLEYSPTSMFWLNNRIAQFAYLRYDAIGAEVRSMIDKHEQER
                     LREIFYVDQKAAELYRINPTTAREYLTDYSLRTAAALFERWKKLDTYLLVKYMDGNTK
                     KQEEDGSFKNNGHRQDMPASPEWGGYNQKWKEAVVKDHGDVLRVP"
     gene            7693..8688
                     /locus_tag="PLI37_00210"
     CDS             7693..8688
                     /locus_tag="PLI37_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOQ57472.1"
                     /translation="MEWYPLAFEPIIKKVLWGGSALAEFKQMDFDSRQAGESWEISAV
                     KDHVSVLENGPLRGKTLEELIRIDPEGLLGSKVLERYGKEFPLLIKWIDTAADLSIQV
                     HPDDAFARAQGQRFGKTELWYVVRAGSESGLIAGFNKELSREEYLRRIADDSIEEVLL
                     RHPVRAGDVFMMPPGRVHSIGKDILLAEIQQSSDITYRIYDFGRKDDRGNTRPLHTEL
                     AAQCLDYKLYSEYKTSYPKLLNQAVELNRNKYFRVNRLELKAQTPLLEIERNPAAGKS
                     FIAYMCIEGALELHCSNGSYGRLSRGRTLLIPAAAGSCRLQCKESALVLEIVADC"
     gene            complement(8805..9992)
                     /gene="dnaJ"
                     /locus_tag="PLI37_00215"
     CDS             complement(8805..9992)
                     /gene="dnaJ"
                     /locus_tag="PLI37_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOQ57473.1"
                     /translation="MAKRDYYEVLEVSRNATEEEIKKAYRQKAIQYHPDKNPGDKKAE
                     EMFKEAAQAYEVLSNPEKRQRYDQFGHAGVEGAAGGGFSGGGFTMEDIFSQFGDIFGG
                     HFGGSSFGGSSFGGSGFGGFGGFGGSREPRVNRGSNLRIKVKLNLKEVAHGTEKKIKV
                     KKQVACAHCKGSGAEGGSAYSTCSTCNGRGSVTRVTNTILGRMQTSSVCPDCQGEGKI
                     ITNKCSHCHGEGIESKDEIINIKIPAGVEDGMQLSLSGKGNAARRGGINGDLIVVIET
                     IDHPELIREGNHLIHNLLLSFPTASLGGTVEIPTVDGKVKVKIDPGTQPGKILRLRGK
                     GLPSVNQYGTGDLLVNISVYIPENLSREERRMIESMEQSDNFKTSPSLSKKIFTKFRN
                     LFD"
     gene            complement(10029..10634)
                     /locus_tag="PLI37_00220"
     CDS             complement(10029..10634)
                     /locus_tag="PLI37_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOQ57474.1"
                     /translation="MNKQNKPKTEHTQEEILQKEQAGEDKSRETKVNSEQKKGKSKQQ
                     EPSKETNAKRIQELESKYNQLKESNLRLLAEYENYRKRSMREKAELIKAGGESVLVGI
                     LSVVDDFERGLAAVQDNSEITAFKEGMELIYKKLTAYLKQHGVVAIETKDQDFDTDLH
                     EAITTIPATDEKQRGKVVECIQKGYFLHDKVIRFAKVVVAN"
     gene            complement(10753..13596)
                     /gene="gcvP"
                     /locus_tag="PLI37_00225"
     CDS             complement(10753..13596)
                     /gene="gcvP"
                     /locus_tag="PLI37_00225"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOQ57475.1"
                     /translation="MNTLLFSERHIGISPEAEQKMLAKIGLNSLEELIDKTLPRDIRL
                     TQEPEPIEPMSEARFAEHIAGLAAQNKLFRSYIGQGWYGSITPAVIQRNVFENPGWYT
                     SYTPYQAEISQGRLEALLHFQTLVCELSGLPLSNSSLLDEATSAAEAAHMMFNLRSRE
                     QERSGANKLFVDRGLFAQSLAVLKTRCSEQGIELVLGDYAAFRPDDSFFGALLQYPNA
                     EGKVENYRSFADKVHKAGLKLAVAADLMALVILEAPGKWGADIVFGSSQRFGIPMGYG
                     GPAAAYFAAREEFKRQMPGRIVGLSRDSRGKTAYRLALQTREQHIKREKATSNICTAT
                     ALMAIMSAFYAIYHGPKGLRDIATRIHGMTSYLNDALEVYGYEQENGSFFDTLKIKLP
                     EGLSTKSFRKVAEAAGINLYYYPDGKTLGLSLDETVDNRALNDLIEVFASAAGNLAVP
                     VEEENWNSICALDDSLLRQEPILQQSVFNSYHSETAMMRFIKQLERKDISLVHSMIPL
                     GSCTMKLNAASEMLPLSRPEFGAIHPMVPKEQAAGYLSIIEKLKQQLCAVTGFAACSL
                     QPNSGAAGEYTGLRVIREYLKAKGQAGRNIILIPDSAHGTNPASAAQAGFSIVQVKCD
                     ANGNVDLEDWENKARENAAGLAGCMITYPSTHGIFEPDVRKMCRIVHQYGGQVYMDGA
                     NMNAQVGLTSPGFIGADVCHLNLHKTFAMPHGGGGPGAGPICCAAHLADFLPTESWDK
                     TKGISVASAPYGNAMLLPITYAYICMLGTAGLKRATQIAILNANYLAACLKSHFSVLY
                     TGAEGFVAHELILDCRPFKEAGISETDIAKRLMDYGFHAPTLSFPVHGTLMIEPTESE
                     SLEELDRFVQAMESIYREIEEVRTGNYPADNNLLVNSPHAEYLLTADEWPYPYPRSRA
                     AYPLPWIEGNKFQIPVSRVDNAYGDRNLVCCTY"
     gene            complement(13605..14831)
                     /locus_tag="PLI37_00230"
     CDS             complement(13605..14831)
                     /locus_tag="PLI37_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOQ57476.1"
                     /translation="MINEGTLRRQHILKQLQSLQADACLLSSSVNLYYLNNRIYKGYC
                     YLPCEGEAIHFVRRPLDLKAEDIGFPAGETGQKSPGSQPDQAHFHLIRKVEQIPGILR
                     ELGMPPAENLMLEGDELSYAEWTRLQQCFPSSRLINGSQALRKARSVKTPYEQSLIRR
                     SAELHAASYRSIPRLFRPGMTDIELSIELERIMRLNGNLGLFRTYGADMDVFMGTVLT
                     GDNALVSSPYDFALGGAGHPSNPIGPNGSPILPGHSVMIDMAGNFTGYLDDLSRCYSY
                     GKLPEKAYKAHQVALELQDRLQERLKEGEICEDLYFFSLDFVASQGLADCFMGKNQQA
                     GFVGHGVGLVINELPVLAPKMKDILEAGMVLAIEPKFVVEGVGAVGIENTFVVGKNQG
                     EKISLLEEEIIDLTNL"
     gene            14974..17850
                     /locus_tag="PLI37_00235"
     CDS             14974..17850
                     /locus_tag="PLI37_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ57477.1"
                     /translation="MPQQRYIEIQGARVNNLKNISVQIPENAFTVITGLSGSGKSSLA
                     FDTLYAEGQRRYVESLSAYARQFLGRMNKPEVDFIKNIPPAIAIEQKVNTRNPRSTVG
                     TSTEIYEYLRLLFARIGKTYSPLSGDEVKKHRTKDVIEYVLKFPEGSRFLVLCPLNLP
                     KGRSLEDHLESFIKQGFSRIEYRGQILSIEDFLSQAGKISPSGIESLYLVIDRLLVQK
                     DDKLVQSRLADSIETAFYEGDHNCLIRMFTNEGSELRHFSDRFEADGMVFEEPNDLMF
                     SFNSPAGACPVCEGFGKVLGLDPDLIVPNKSLSVYDDAIVCWRGPKMSEWKNRLVENA
                     AAFDFPVFKPWYQLSESQKRLVWTGNEYFQGLDDFFRMVESNQYKVQYRVMLSRYRGK
                     TICPECKGTRLKKEASYVKVGGRSISELVDLSILELNSFFEQLQLSANEKLIAERLLT
                     EIRTRIKFLLDVGLSYLSLNRLSSSLSGGESQRINLVTSLGSSLVGSLYILDEPSIGL
                     HARDTALLIKVLKELKELGNTVVVVEHDEEIIRAADYIIDIGPKAGRLGGELMYQGSV
                     SGLINHSESVSVNKQEKTSRKKPVKENPVQFQSHTLNYLSGKEKIPLPELRRPWNNYI
                     EIVAACHHNLKNINVKFPLNVMTVVSGVSGSGKSSLVRDIFYVALKRICSETTDSCGS
                     FGELRGDIHLVHNIEFVDQNPIGRSSRSNPVTYLKAYDEIRKLFAEQALAKQMGFNAS
                     YFSFNTDGGRCEACQGEGVERVEMQFMADLEMVCEHCHGKRFKSDVLEVLYREKSIHD
                     VLEMTVDEAIEFFGQGRQALEKKIVKRLLPLQEVGLGYIKLGQSSSTLSGGESQRVKL
                     AFFLSNEREEPGVYVFDEPTTGLHFHDIHILLKALNALIAKGHTVIIIEHNVEIIKSA
                     DYVIDLGPEGGDQGGYLLFQGKPEDLLQCKESYTARALKKHLKG"
     gene            17947..18876
                     /locus_tag="PLI37_00240"
     CDS             17947..18876
                     /locus_tag="PLI37_00240"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOQ57478.1"
                     /translation="MDSIAVDSTAKSSLPFEPAVVTETVTQTVESMNQFWHKARDFFT
                     DVLLANVPRIIIALVLLWVGFRLVNYLTRMVRKIMQAREMDKSLQDFLGSLIGIILKV
                     LIIMSVMQMIGVAVTSFIAILGAAGLAIGMALQGTLQNFAGGVIILLLKPFKVGDFIE
                     QGSISGTVTSIQIFNTVLSTPDNKVVIVPNSQLATNSLINYTKSEVRRVDVSVGIRYD
                     ESINKVRKLLLDLASAYPEVLKEPAVPVVHVTSMGESSVNLQLRLWAKTEDYWPLYFS
                     LNQAVLDEFSKQDIKTPFNQLSVRFEEGVSPTK"
     gene            18888..20528
                     /locus_tag="PLI37_00245"
     CDS             18888..20528
                     /locus_tag="PLI37_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009039061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOQ57479.1"
                     /translation="MKSKFLLLALTAGLLFSFAPERKIVVYTIGDSTMANKPLDNMSL
                     ERGWGQVLSAYLDSRYVEVDNHARNGRSSLSFRNEGLWAPIYDKIKPGDYVLIQFGHN
                     DEKPSPDRHTEPGSTFNEQLRRYVLETREKGGTPVLLTPIVRRKFDEEGRLIPTHGDY
                     PQAVRDVAAELDVILIDHNVLSRHLVQDLGPEASKELFMWVPPGTNPALPQGKEDDTH
                     LRALGAKRIARLVLNEIKEKIPALAVHVREYDFVVAKDGSGDFFTVQEAINAVPDFRK
                     NGRTTIFIRNGVYKEKLILPESKINVSFIGESVEHTILTYDDYAQKLNIFGESKSTSG
                     SSSFFMYAPDFWAENITFQNSAGPVGQAVAIFVSGDRAVFKNCRFLGFQDTLYTYGKH
                     SRQYYENCYIEGTVDFIFGSSTAVFKDCEIRSKRDAYITAASTPQGRDYGYVFINCKL
                     TADPGVEKVFLGRPWRPYAQTVFIHCEMGAHIRPEGWNNWGNVSNEKTAFYAEYGSSG
                     PGADISGRVRWAKKLNKKKLAAYSIEKVLAGDDGWNPL"
     gene            complement(20525..>21373)
                     /locus_tag="PLI37_00250"
     CDS             complement(20525..>21373)
                     /locus_tag="PLI37_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HOQ57480.1"
                     /translation="VGILVTLIPNLDNLPELNRAGLRNLHPEAARLPIFPMMFITVAC
                     GAISGFHATQSPLMARCMKNECDGRKVFYGAMVAEGCVALIWAAAGMAFFGGLDKFQL
                     FMSENGNSAAIAVDTISRGWLGKVGGVLALLGVVAAPITSADTAFRSGRLIMSDLLHY
                     DQKKMYKRILLSFPLFAAAFVLLQIDFEIIWRYFAWCNQSLAVFTLWAISVYLFREGK
                     NYFIALLPALFMTVVCTTYILTSPEGFRLNNYLSVGIGLVVAICFSVLFFKKNFKNSG
                     FSFKKR"
     assembly_gap    21374..21473
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21474..21820)
                     /locus_tag="PLI37_00255"
     CDS             complement(<21474..21820)
                     /locus_tag="PLI37_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOQ57481.1"
                     /translation="MIGFVACLTALIAGYFLYGKYLEKVFQPEKTRTTPAFSLRDGVD
                     YVPMPTWKIFLIQFLNIAGLGPIFGAVMGAMYGPVVFLWITLGTIFAGGLHDYFSGMI
                     SERYNGESLPELVG"
     gene            21909..>22687
                     /locus_tag="PLI37_00260"
     CDS             21909..>22687
                     /locus_tag="PLI37_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOQ57482.1"
                     /translation="MKKKKFLTCDGNQAAAHISYMFSEVAAIYPITPSSTMAEYVDEW
                     AAAGRKNMFGETVLVQEMQSEAGAAGAVHGSLQAGALTSTYTASQGLLLMIPNMYKIA
                     GELLPCVFHVSARTLASHALSIFGDHQDVMACRQTGFAMLAEGSVQEVMDLSAVAHLA
                     SIKSRVPFVNFFDGFRTSHEVQKIEMLDTEDLSPLVDREALAAFRARSMNPDKPVMRG
                     MAENPDHFFQHREALNPFYEAVPDIVAGYMEEINKLTGREY"
     assembly_gap    22688..22787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22788..25388
                     /gene="nifJ"
                     /locus_tag="PLI37_00265"
     CDS             <22788..25388
                     /gene="nifJ"
                     /locus_tag="PLI37_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOQ57483.1"
                     /translation="PKTVKRIAVLDRTKEPGANGEPLYLDVVEAFAKNDALGLDKMPF
                     IVGGRYGLSSKDTTPAQILAVFENLSLPEPRNQFTIGIVDDVTFTSLPQEEEIALSGE
                     SIYEAKFYGLGADGTVGANKNSIKIIGDNTDKYCQAYFSYDSKKSGGFTCSHLRFGDT
                     PIRSTYLVNTPNFVACHVQAYLNLYDVTKGLRQNGTFLLNTIWEGEELAKHLPAKVKR
                     YFAKRNISVYYINATRIAQEIGLGNRTNTILQSAFFRITKVIDVDLAIDQMKKFIVKS
                     YGNKGEEIVRKNYEAVDRGGEYKQLKVDPAWANLPDDPAVINNDPEFINKVVRPINAQ
                     DGDLLPVSAFKGIEDGTWMPGTARYEKRGVSAFVPEWIAENCIQCNKCAYVCPHASIR
                     PFVLDEAEAAAAPGFETIEMKAPATMKGMQFRIQVSVLDCLGCGNCADVCPGNPRAGG
                     KALKMVDLESQMSEASNWEYCVDKVKSKQHLVDTKQNVKNSQFATPLFEFSGACSGCG
                     ETPYLKLLTQLFGDRQIVANATGCTSIYSGSVPSTPYTTNELGHGPAWANSLFEDFCE
                     YGFGMQLAYEKMRERLVKLMSDAQSCSRCSEEQKALFAEWLTDREDGDKSRVLADKII
                     DAVKGCDCDYCKQISSLSKYLVKRSQWIIGGDGAAYDIGYGGLDHVIASGKNVNILVL
                     DTEVYSNTGGQSSKATPLGAIAKFAAAGKRIRKKDLGMIATTYGYVYVAQVAMGADQA
                     QTLRAFREAEAYDGPSLIIAYSPCINHGLKRGMGKSQAEAEAAVACGYWHLWRYNPQL
                     EAEGKNPFILDSKEPDWSKFADFLKGEVRYSSVIKQYPDEAEELFKAAEESAKWRYNS
                     YKRMAGLNWE"
     gene            complement(25561..26172)
                     /locus_tag="PLI37_00270"
     CDS             complement(25561..26172)
                     /locus_tag="PLI37_00270"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOQ57484.1"
                     /translation="MNKLFLREINDPKTLRAMEAVDRKFFVPEKLQSHAYSDTALPIG
                     LGQTISQPYIVAYMTASVKAGDCDKILEIGTGSGYQTAILAELAREVYTIEIIPELGI
                     KAKNVLDTLGYKNIRYKTADGYRGWQEMAPFDAIVVTAAIAETPPDLLDQLAENGRMI
                     LPLGPEGDTQYLVLIEKKAGQFTYSRKLAVRFVPFTRSKTDNK"
     gene            26246..30277
                     /locus_tag="PLI37_00275"
     CDS             26246..30277
                     /locus_tag="PLI37_00275"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOQ57485.1"
                     /translation="MFAFIILFVSSMTINPFSIAYIASALITVGLMVFLFVKRKESLS
                     TYVYMLLTCTAVWSFFYGLELGSSNSEDIRFYVQIEYAGICLLPIFYLFYILEYTGRE
                     KHLNTSLYPLFFIIPLVTISMLMSNDVHHLFYAQSSFKTAGRYYYHALVPGPFYYLHV
                     VYSYGLIVLGIVLLLKSYRGQSKRQRNNILILLSGTIIPFLTSIAYILGFKYAGFIDF
                     TPLGFLVMSIIVAYAASTNRLFEMTPLAMSYLYEIIPDAIFVLDRDSAIINTNPSAQK
                     LLKSGIMEECPESEKCLHFEEFSDVEEIKYKEIKAGDKHFFITKKAVVGGNNQQIGLM
                     LIVKDVTEVKNLEHMQEILMSIALNFINTPLDNYEKTVKASLEQMGRYVNAERVCIAS
                     FNYGKGVVDLIHEWRRPGVDSIIDEIRIITLEDIKELIEIHKRRESFFVEDAKVLIEG
                     TPLYDLLNLKKERSFLAIPMFEASNLIGFLGFNWSYKNQRYSFEEQQLLQLFSEILVN
                     LISREEANIIIKQKSEIEKFTSEIFSDFIQVNIQNIQEVAPKTLEKLGRFLQVEGAFL
                     IQFGTDRNLQIFKWSLIPRSEPQQIALEDHAWFSKQVNKGVLSIDNLEDLPDEAELEK
                     NDLKALGVKSMIAVALRNKNQTIGVIGFQMFGSYRRWTGADADMLISFSNFMAEVLFK
                     VEREKELIQARNLAEAASKAKSEFLSNTSHEIRTPLNGMIGFTELLRNTKLNKIQLEY
                     VENAIMSANTLMGVINDILDFSKIESGKLELDIVHSDIIQLVEKSSDIVKIMASQKGL
                     ELLLNIQPDAPRYAYIDPIRLKQIMVNLLSNAVKFTTEGEVEICLKFKEIDEFEGQFT
                     LAVRDTGIGIRDEDKSKLFKAFSQADSSVTRKYGGTGLGLVISNSLAEKMGSKIDFTS
                     EYGKGSVFSMSFVSRYERKQAEESMGSLPVKRVLIVDDNANNRKILEHNFMYWGLDFS
                     SAGSGKEALAVLEKDSDFDLILIDYNMPEMDGLTAVAAIREKKLLNPEKQAIILLHSS
                     SEDHTINERARKLQIWRTVIKPLKASDLLFSLKNLSPVSPATDLVKAPESPLVREKPV
                     DFSKQFCVLVAEDVKMNMMLVVQLIRKRFPNAKILEAENGKEVLELLENNLPDLIFMD
                     VQMPVMDGLDASRNIRTHNNPRVRSLPVVALTAGVSKSEQELCKLAGMNDFIPKPIDK
                     KILYRIISKYVASASGKWQAEVEEGEKPSDISLHFNKNNLLDKIQNDKDLYKQLMFQS
                     LKEFDKDVNELLENIETGDPDRIKSGAHRLKGSAFNMEFTRLGNLAKVVEQNASDVFL
                     LESEGEKLKTEWLTLKDIIANEI"
     gene            30284..33355
                     /locus_tag="PLI37_00280"
     CDS             30284..33355
                     /locus_tag="PLI37_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ57486.1"
                     /translation="MKTSQTETEQRLSIRRTSHKNRLVIIGIVCSFILFVFYAFNSWR
                     AADKDLEKGALAYAQTIEVALNTVRLEFLQANEGVESSDYYRIIKDKLIELRRMNPNT
                     RTIYLLKQEEGEVIFLVDSEPANSIYYSSPGREYYDTDTAILKAFDRAEPVITDHHTD
                     SLGKWKSVVVPLRSFRNSAVYALLGIDYDVNRWKKLVLAEASEDIIYAFAVVLLFVSL
                     LLIMSKSNSLNKQEKLRQIAEQQAMALLHVVEQSDDIITVKDSDLRVFAANKAFARSA
                     GYDDFTEVIGKTDAEIYNIPPWEEPVRTYMDDERRARSLKPGEYLLREEELITHTGER
                     RTILTKKYPIYDTSGNVLFTSNISRDITERKKMEIELKNSKELAESASRAKSEFLSNM
                     SHEIRTPLNGVIGFTELLSNTPLNKMQKEYLDNAIVSANSLLGIISDVLDFSKIEAGK
                     LELDMVSTDVIRLVESAADIIKIQASHKDIELLLDVQPDLPRFMTLDPLRFKQVLVNL
                     MNNAVKFTHAGEVELKLNFIRLDENKGQLTVSVRDTGIGIKEADKSKLFKAFSQADTS
                     TTRRYGGTGLGLIISNSIVKKMGGEIQFESKIDEGSRFYFTLELNYDDDTTDVLSPIK
                     DIKTVLFVDDNENNRLILERTFEYWGIEFTGCDSGLKAIKLLQSGKHFDLLIVDYHMP
                     QLDGLETIKLLRKDERFAAGKLPVILLHSSSEHDTIHCAAKELNILYTLTKPVKAVEL
                     YYYLQKLQAGNGDTYEKYLQKLSRKEESSVRILENKLKIIITEDIKMNRVVIANILKS
                     FLPNAELYEAVDGQEAIEMFKNVDPDLILMDVQLPVMDGLEATRRIRNSDNPRAKELP
                     IVALTAGVSNQEKENCFQAGMNDFLPKPIEKEALFQVIIKNVIGPQKATYQTDDLLEP
                     DVPHFDEDKLLEKIDNDTDLMKSLLEMAAEEYPKFLQQMKTACENKDACSVKSIAHTL
                     KGSAYNLEMTAMGDIARKMEFHHEDPEVLLLLLESLEKEWKNVQNILEQKQ"
     gene            33454..34548
                     /locus_tag="PLI37_00285"
     CDS             33454..34548
                     /locus_tag="PLI37_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOQ57487.1"
                     /translation="MSHDKKHLIYVIDDNPMNLLLTSKILESYGFETQTAESGAAALQ
                     LIRQQRPSLILLDINMPNMDGYEVCRRIKAKEDWADIPVIFLTANTQTENLVEGFNVG
                     GVDYITKPFRREELWVRVSTHLELAESRRTIIEMNRSRDRLYSIIAHDIRSPLSAILQ
                     TLEAIDQGYIGLEGEELREIIKLLGERTKETSTLLNSLLQWTRVQTDSQRLEFKSTDL
                     FVLIQSCVDLLRPNAEQKELSIEVEVEEELKAWCDELSIHTVVRNLLSNAIKFTPRGG
                     KVRIGAGKKDGQAMIYVADNGVGMDSETVERIFAKDQHFTSSGTENEQGTGLGLMLVK
                     DFVKKNNGSLQVDSEPGEGTEFRVFLPLNN"
     gene            34556..35923
                     /locus_tag="PLI37_00290"
     CDS             34556..35923
                     /locus_tag="PLI37_00290"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOQ57488.1"
                     /translation="METSGRNILIIDDDPVYRRLSSSILKERFTVFTADAPSAGFEIL
                     KNEKIDYVICDYRLPEMDGLGVLERIKTDHPHIEVIMVSDSGNMDTVIEALRRGAVDY
                     FKKPFTPADIWMSIEKTQRFTKLKKDFQLEKNKSSRLRQWVDKEVSIQMIGESPAIEE
                     IKNQVRLVARTPDTSVLIIGESGTGKELVARGIHDLSARRDELFGAVNMSAIPESLFE
                     SEFFGHKKGSFTGAIADKAGWFETTDKGSLFLDEIGDMPHNLQVKLLRVLEEKKYIKV
                     GTQKEQTFDIRIIAATNKSLEQISNPKFFRTDLFHRIGTFIIHLPPLRKRKEDIPLLV
                     EYFVEQFSEKMAKSNIQVAPEVFDLLQSYSFPGNIRELRNLIERAVILCKDNIIKAEN
                     FGIIKKLAHSAPQEEDSYDLQEVEKRAISKALDISGDDQEEAAKLLNIDSKTLSKKMK
                     QHGLL"
     gene            35938..37344
                     /locus_tag="PLI37_00295"
     CDS             35938..37344
                     /locus_tag="PLI37_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOQ57489.1"
                     /translation="MKKNSPTLLAILIIITTVVLLLMYRSPNNIYRPDEKYRAILRAV
                     KAPVLPEKTLSILDFGALGDSVTDCRPAFEKAMQACRELGGARIVVPRGKYFLDGPIH
                     LVSNVCLDLREGAELFFGSNPRSYLPVVRSSWEGTFLYNYSPFIYGYQLKNISIIGKG
                     SIDGRAAEGFAEWADLDDDDQALSREMNYAGTPVEERIFGEGHFLRPQLIQLFECKNV
                     LIEDVMLKDSPFWCLHLLKSENITVRGIRFSAFNKNNDGIDPEYSRNVLIENVQFNNS
                     DDNVAIKAGRDYEGRKTNTPSENIIIRHCRFKGLHAVVIGSEMSSGVRNVFVHDCDHG
                     GYLKRGIYLKSNPDRGGYIRNVHIDNLKLGTVEDCFFITSNYHNQGKGFETDIRDIHV
                     NNLHCKEAENAGIVIQGFETKKVRDIYLSNVNIEKAAIGLFMEHAENIVLDNVISGGR
                     VGAPSTAKKTDLQRIERE"
     gene            37397..38386
                     /locus_tag="PLI37_00300"
     CDS             37397..38386
                     /locus_tag="PLI37_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57490.1"
                     /translation="MIIESRAYARAGLLGNPSDGFFGKTISIIIRNFGAGVLLYESPE
                     LVIEEDEEDRFRNLRHLTETIKKTGYYGGKRLVKAAVKKFSEYCDQEGIRLPARNFTI
                     RFSTTIPRQVGLSGSSAIITATFKALMKFYEVEIPQEMLPSWVLKAESEELSITAGLQ
                     DRVIQVYEGCVYMDFDREFMLKHGRGRYEVIDPALLPKFYVAYKTRLSKVSGAVHNPI
                     REKFEAGDPLVCDTMAQIAEVAEQGRTAILNKDYDTLDKLINLNFDLRRKIYKISPAH
                     LELVEAARSCGVSAKFAGSGGTIIGMYRDEESLHRLFIKMKSLNARVIKPYIM"
     gene            38408..39286
                     /gene="galU"
                     /locus_tag="PLI37_00305"
     CDS             38408..39286
                     /gene="galU"
                     /locus_tag="PLI37_00305"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOQ57491.1"
                     /translation="MIRKAVIPAAGLGTRFLPATKAQPKEMLPIIDIPTIQYVVQECV
                     ESGIEDILIITGKGKRSIEDHFDRNLELEERLLKEREDEYWYNELRHIDSMANIHFVR
                     QKEQNGLGDAIYYARFHCGSEAFAVLLGDSVNDSVIPITQQMIDTYEQYRHTVIAVQQ
                     VDKDKVDRYGIVGGTFLSDRIMQVSEMIEKPAPDKAPSNWAVAGRYILTPEIFQAIEQ
                     TPRGKGNEIQLTDALRILLQTESIIAMKIEGRRYDIGNKLDYLKTTVDFALKRKEFAN
                     DFAAYLRKKLNEFDKV"
     gene            39346..40965
                     /locus_tag="PLI37_00310"
     CDS             39346..40965
                     /locus_tag="PLI37_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HOQ57492.1"
                     /translation="MKTFISLLITSLLSLNLLHAQYISKVWKGDLGDGRYQNPIINAD
                     YSDPDVCLGADGYYMVASSFNCVPGLPVLFSRDLVNWSLVGHALSRQVPEDFYNKVQH
                     GSGVWAPSIRYHNKEYYVYWGNPDFGIYMVKASNPRGPWTEPHLVKAGKGLIDVCPLW
                     DEDGKAYMSFALAGSRAGLKSVLLVAPMSPDGKSLIGEPRIIYDGHEKNPTIEGTKFH
                     KHEGKYYIFAPAGGVSTGWQVVLRADQPYGPWQVRTVMHQGKTEINGPHQGAWVRTPQ
                     NEDWFIHFQDVGVMGRVTHLNPMQWKNGWPVIGLDGDNDGIGEPVSIYRKPVVPVRLP
                     VATPAESDEFDKATLGLQWQWQANPDFRWYYLDPASGGNLRLYSFYTPGAISLWDVPN
                     LLLQKFPAPNFKVTAKVRFEPDKRYKGERGGLVVMGMDYAVLCIENTEDTLLLTQNTC
                     NKADNNGTERIVAAVPLAQQDIYLRVEVKEGQHCAFHYSLDGQKYIRVGTEFKAREGK
                     WIGAKVGLFCTRPQESNDGGWLDVSYFRIEP"
     gene            40965..>41290
                     /locus_tag="PLI37_00315"
     CDS             40965..>41290
                     /locus_tag="PLI37_00315"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOQ57493.1"
                     /translation="MKKHLLCCLLALLGFALPAAGITISGYISDEETGETLINATVYD
                     SISGKGAVSNLYGFYTLTLPEGPVHLIYSYLGYKPQRFAFWADSDRQLTVRLQSGAEL
                     EEVVVSG"
     assembly_gap    41291..41390
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <41391..43211
                     /locus_tag="PLI37_00320"
     CDS             <41391..43211
                     /locus_tag="PLI37_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ57494.1"
                     /translation="YKGNFPARFGGRLSSVVDIRMNDGNDKSYHGNVSVGLISSKFNF
                     EGPIVREKTSFNLSFRRTYADLLLQPILQATRRGMDELDKLSAGYYFYDFNAKINHKF
                     SDRDRLYLSFYSGDDAIYAKVRNKYTESKTYKSTSAIGLDWAWGNLITALRWNHIINS
                     KMFLNTTASYTRYRFDMSIGLKDENWEYQPPSYLATDIMMGYRSGIEDYTIKADLDYT
                     PNPGHTVKLGANYTYHTFKPGVTVAKFRIDDDSVHQNLLDTVFGDSFVYAHETMLYAE
                     DNIDLGRYVKANLGLHYSTFFVQQQFYHSLQPRLGLRFLFSDNFSFKAGYAMMNQYIH
                     LLSNSDISLPTDLWVPVTKRITPMNSHQVSAGLFYNLKNLVDLSVEGYYKSMHNILEY
                     KDGASFMGSTTGWEDKVSMGRGWSYGLEFLAQKSYGRYTGWIGYTWAKSERLFDRPGQ
                     ELNNGKVFPAKYDRRHDLSITASYKLNDRIDFAASWVYSSGNCATLALQNYEGSPVPD
                     SYQYYFSSLPYIESRNNYRFESYQRLDIGVNFRKKLKRGTRTWNISAYNAYNRNNPFF
                     VYPSHSERYDPVSRQYVTKRALKQVSIFPLIPSISYSYSF"
     gene            43221..44180
                     /locus_tag="PLI37_00325"
     CDS             43221..44180
                     /locus_tag="PLI37_00325"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOQ57495.1"
                     /translation="MKKAILYLTVFMLFAACEKEVRFKGEVTEPLLVLNGFAQPDSLL
                     VIRLTASRFFLEEVDSFVTVKDAHLRLYVDGTYKEDLQSQKNGYYISSYRPKQGEVLS
                     IEAEAPGFESVRAEALLPVVAELLKVDTSITLEGELYDLSYDWDPEVGDYIIDTIGLY
                     TNYQVSMTLSFRDPPTPGDYYRVVVKHFYGKEYYDGYYDFTVENFYTAEMGDLLGPAE
                     TQNEYQIYADELYNGKTVDLKLSFAAHTYRDYQGELPNSTTSIQVQLQNISPSYYLYL
                     KTFSEYNNSIDFFSEPIQVFSNVSGGIGIFGAYSSNVIELKFN"
     gene            44243..45022
                     /locus_tag="PLI37_00330"
     CDS             44243..45022
                     /locus_tag="PLI37_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HOQ57496.1"
                     /translation="MKKPAFKNIALLCGSILALLGMSCTQKEDIRLFVAGDSTAQSYR
                     VEQTLMRGWAQYLELFFDERVQVENRSMAGRSSKSFREEGRWNKILEDLRKGDVVFIQ
                     FGHNDTSTKPERHASPADYRQNLRRFVQEVREREATPVLLTSIVLCTFNDSGQLVDRR
                     LKEYPQIVRDLAAEEKVILIDMFRLSFDLLQELGPEEARKLYMFLEPGEDPSRPQGSN
                     DDTHLRERGAKQLAALVAEEIRQARIKPLYRYLLDKEEWPQ"
     gene            45084..45159
                     /locus_tag="PLI37_00335"
     tRNA            45084..45159
                     /locus_tag="PLI37_00335"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45117..45119,aa:Gly,seq:ccc)
BASE COUNT        12032 a        10358 c        11082 g        11400 t
ORIGIN      
        1 acataccaga tgtaatgggc attgaccgtg gcttgggcgt aggcttcggc cgaatcgaag
       61 agccggggat cggggcagta tttgtcgagc agaaaacccg agagcccgaa ggcgaacaaa
      121 taggaaatga aggaatgtaa atggctgaat cctaagtaga ggccttcttc gcctttgggg
      181 gcctgcaagg aaaaatactc cagaaaacgg ggcgaaataa agcattcggc cagagcttgc
      241 atggcgatac cgatcaccat caacagggcc accgggtgca ttcccaaaac gggagtgctt
      301 cccagttgat gccccagcga cataaccagc gccgagagcg gaatgatggc catgcctatg
      361 gtcatggaag tgagggccgt ttttttggcc atcagtgaag tgataaaatt gaccagcaaa
      421 aacactacca gggggtttac gttggcgtac cagccgggtg aagcactttc gccgatcagg
      481 cggatcacgt acttgggcat ggtagcgtac atttggcttt gtatcatcca gaatccgctg
      541 ataatgagaa tcaacacgat cagccgggca ttggtaaata ctttaaccaa tccccgccag
      601 atttcacgca gcgatttgcc ttcgccctga gtctgggaag atttgtacag aaagaaaacg
      661 gcaatcagcg ccaacagagt catggctgcg gcaaaatagt tcaggtaaac caggcccata
      721 tcgcctaagc ttttgcgcag cggatcgacc accgattttc cggtaaaagc tccgatgttg
      781 accatcatgt agaagatggc gtaaccccgt gcccggtttt cggaagtggt ttcgcgggca
      841 accgtgcccg aaatcaccgc tttgacgaag gctccgccaa cgacgatcaa aatcatgacc
      901 ggaacaatag accaacgcag gggagattcc ggcagaccgc tgaaaacggt ggtatcgccg
      961 taacttacca gcccggcgct ttcgagcagg gtgggcagga ggcccaggcc aaagtaaccc
     1021 aggctaagca gggaaaaagc caggataatg gactggcgaa aacctatgcg atcggcgtaa
     1081 gcacctacaa atgtgggcag aaaatacaaa ctggccgaaa aaacgcccga aatcaggccg
     1141 gcttctatat cggagaagcc ccagatgttc gacaggtaga gggttataac aatgaaaacg
     1201 gcgtagtagg ccagacgctc aagcagttca acaacattgg caacccaaaa ggctctggta
     1261 aattttggta gagacataaa acggtattaa aaacgataag aaaaaaagag tcctcccttg
     1321 taagccggca tccactcggc gctgcggttg ggccggcctt cgggaatgct ggggtcggta
     1381 tacaatactc cgtttttata gccttcgtag gtggaatgtt gccagcccag ccccaggttc
     1441 aggtcgagcg accagcgttt ggacaagacc cattgatagc ccaggttcaa tcccagcatc
     1501 acattgtgcc cgtattgaac cgagttattg ttgtagctgc cgaaataacc cggagctatg
     1561 cttttattca ttttccagac accccagcct aagttgggcc cggcgtagaa accccggtaa
     1621 acttctttgg gataaaaacg tccttcgata aaactaagtc ccaaaaccag cgggaagttc
     1681 aaaaaaggca cgtgatcgtg gtaaaacacc cccatgcctt ccaactgaac gctgatgttg
     1741 ttgtgtacgg caaattccac ggccggattt accacaccgg ccagcagcag accgttgagt
     1801 ttgacggcta cgcgtttgcc gggaaggcca tcgggtgctt gccggcccgg gttttcagca
     1861 gaggtgcttt cgacctgttc ctcttctaca gcaaaagtgg ctgtcttgtt ttgtccgctc
     1921 tgcttgtttt cggccggaac ggggctttga attaaaagaa agacgacgac ccagaggtag
     1981 aataaaaata ccgggcgttt gggagagaat gcaatcatac aagcagctta aacgttggga
     2041 gagtcggttg ttttcttcga tgttttttta agcacgataa agagcagtat cagcaggagg
     2101 gcagcaattc cgccgtcaat gaaaagcttt gttcgctggt gcataccggt ggacttgcgg
     2161 atgaatttgc tgatgtcttt gagtactttg gaagagaaag tttcctctat attggcgtat
     2221 tcggaaggag atccggtgtt ggcgttttgg aacaggtggt tgaggttggg gtattttatg
     2281 atggtgagtt tgggattctt tccttccgtc aatgcctgct gtatggccgc caggttgatg
     2341 tcggcattca cctgaagatc tttttctccg ttcagggcca gtaccggaca actcacttct
     2401 ttccaggctt gtgcgggatc aaagccgaga aaatggaaca tccattcctc gcccagttgt
     2461 tgtacgctgg cctggatgta ttcttccctg ctcatgccgc ccagggctat ttcgctctgt
     2521 tcgggattga gttgctgcaa agccccttcg atgataccgg cgatgctgtc cggctcgttt
     2581 tccggcaagg cggctatacg atcaaagata tattcggcga gtattttttg aacctccaaa
     2641 gtatcttcgg ctgttccttc ggctttagcg atatcgaagc tttgtttttt gatgatttcc
     2701 gcacccgtca cacccgggcc ggccagggaa acaataaagg ctataccgga gtcgatggag
     2761 gcaaccatgg gagcaatcag tcctccttcg ctgtgtccga tcaatccgat ttgtcggtag
     2821 cccgatgctt tgaggaagtc aacagcggcc gcagcatcca gggcattctc gtagctgctg
     2881 ccttcgtatt gatcaccacc cgattccgcc gttccccggt cgtcgtaacg caaagaggca
     2941 atgcctttgc gggccaggta gtcggcaacg actaaaaagg gcttgtggtc gagcagttcc
     3001 tgatcgcggt tttgggctcc gctgccggaa ataaacaccg caacgggagg attgttgcct
     3061 ttacggggct taaaaagggt gccggccagt tgatagtctc cacccccttt gaaccggacc
     3121 tcttcggatt ggtaggggaa aggaggtcgg ggctcctgag gacgtcgtac tttctgttct
     3181 gtgctgtcgg tcaacggctg atgcgtcata tacagcccgg tcttgatagc ggcctgttta
     3241 aactcgccgt tgagattacc cgcagaatcc agtttggcgg tgtaggttat attggagact
     3301 ttcaggatga gtacctggtc ttcgtaggtg gccgattgca tgggaatccc aaaggctttc
     3361 tggtcgggac tgtccatggt ggccgaatat tgtccggctt cgttactgat atggaagtta
     3421 agtcgtagtt cttgccccat aacggaaatg cggccatacc aacttccggt aacatcttga
     3481 ctctctgtgt ttgcatcggc ctgatcctga ctccatgtgt ttgctgccag caggcacagc
     3541 accaacaacg aaaatatgcg tttcataagt ttgatatttt ttgcaaatat aacaaatccc
     3601 gaagtaaaac gctcttagac ttaatatata gaccggtatt taaaaaaatc gtacttttgc
     3661 ccggcatcac atgttagaaa gtgaaaaaac tcagtatact cgaaatgaac cgcctgtcgg
     3721 cggaagattt taaaacagcg cccaagcttc ccctgacatt ggtcctggat aatgtgcgca
     3781 gcttgcacaa catcggttcg gtgtttcgta cttcggatgc ttttctggtg gaaaaagtca
     3841 tcctctgcgg tattagctcc tgtccgcccc agcccgagat tcacaaaaca gctctgggag
     3901 ctgaatttac agtagactgg atgtacgaag aggattgcct gcaggcgatt aaagctttgc
     3961 gtgaacagag ttatattatt tatgcagccg aacagaccac gggcagtatt tgtttgcagg
     4021 atctccggct cgatcccgga caaaagtatg cgctggtgct gggcaacgag gtgaaagggg
     4081 tacagcagga agtcatcgat ctctgccatc attgcgtgga aatacctcaa tacggcacca
     4141 aacattcgct caatgtgtcg gtggcgtcgg gcatactggt ctggagcttt tttgaacaat
     4201 tgagaaaacg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggctgggtaa
     4321 acccaggttt ttgagcgacg aaaagatcaa cgaatttcac gccaggattc cccacaacat
     4381 cagccatttt atggatgtgt cctatccctc cgacgaaagg ttcattcgca gtttgatgtg
     4441 caatattatc cgtcgctctt gcgatcaggg tctgatgatt tccacttacg gtactgtgtc
     4501 ggtgcgttgg cgcaacgacg actttttgat cacttcgccc aatgtgccgc gttgggatgt
     4561 acagcccgaa gacattgtgc aggtcagaaa cggtatggcc gaagccggca agactcccag
     4621 ccggtcggtg gctttgcatc atcgtatcta tcaaaagaat ccgcagatca attccatcat
     4681 aaccactcag ccgcccaacc tgatggcttt tgccgtttcg ggtaccaaat tcgatgtccg
     4741 gaccattccc gaaagctgga tctttttgca agatgtgccc agtattcctt ttgggatgca
     4801 ctacgaggaa atcgatcggg tggccgatat gttgggccgt aaccgggcta tcctggtcga
     4861 taacgattgc gtcattgtga ccggcgataa gttactgcag actttcgact acctcgaagt
     4921 agccgaattt agtgcccact cgttggtaat ggcttcggcc atcggtcctt tacaacccat
     4981 cggcgaagag gagatcgaag aattacgcaa ggctttcaac gtaaaataat aaggcggctg
     5041 aacttatgag aattatacgc agcaagcgca acgacacagc ctggcatttg gctgccgaag
     5101 aagtactttt ccaaggcaag gatgcggttt tgttgctgta tatcaacccc ccttcggtag
     5161 taataggttg taaccagatt atggaaaacg agtcggatac ggcctattgc cgggaaaaca
     5221 acataggtat ttatcgccgc atttccggcg gaggtgcggt ctatcacgac gaaggcaaca
     5281 tcaattactg ctttattcag gacaaaaatc cgcagcaatc ctccctgaac ggagcttttc
     5341 tggagccggt ggaacagatc ctgcaatcgc tgggtctgca agtgagcaga gcccgtcgca
     5401 aagacctctg ggcgggagga tacaaaattt cgggaaccgc ttcgcacatc ggcaaaggca
     5461 gggaattgca tcacggcact ctattgtacg acagcaatct ggaagccttg cacgcagctt
     5521 taggcctgat tcccggagaa aaagcagaac aatcgccggg gaaaaagcaa cagccgggaa
     5581 gcagaggaat agtttccgta ccttcgccgg tggccaatat ccgaagtttg tacccttcgt
     5641 ttgcagctct gtctacgcag gaattttttg ctgctttttt cgaggcggct cactgctact
     5701 atggacagtc tgtttacgga cagtctgttt gggatgggga agagcaaggc ttaaaagagg
     5761 gacaaaagaa accaaaagaa gacaaggaga gacttcaagg ttttagagaa aaagagttga
     5821 ttgaaataga ggccctctgc aggcaaaaat accggaacaa agcctggaat gaattgaaat
     5881 aaccctgata agcagagaag taaagggaag agaaaacaac acaaggaaaa aagtaaaata
     5941 gtaagcgaca aacaaaagag atataagatg gataaaagaa taaatagaaa tcccgggagt
     6001 atgaagaaaa gtatgctcag ccttgttgcg gctgtcctga ttgcgagcct gcaagcggtt
     6061 tatccctgca cgaatttgat tgtgaccaaa ggggcttctg ccgacggttc ggtaatggtc
     6121 acttactcgg ccgattccca ccagttgtac ggggaattgt atttccggcc cgcagccgat
     6181 tatccggcag gctccatgct tcagatttac gaatgggata gcggtaagta cctgggtgaa
     6241 atccctcagg cagagcatac ctattcggtc accggtaata tgaacgagca tcaggtggtg
     6301 ataggtgaaa ccactttcgg aggccggccg gaattttacg attccacggg cattatggac
     6361 tacggttctt tgatgtttgt ggcattgcaa agagccaaaa ccgcccgcga agccattgcc
     6421 gtcatcgacc gattgatgca ggattacggc tatgcttctt cgggagaatc tttttccatt
     6481 gccgatcccg acgaggtctg gattatggaa attatgtgca aggctccgcg ttacaacaag
     6541 aaaggaaaaa atctgaataa aggggccgtt tgggtagcca agcgcattcc cgacggacat
     6601 ataagcggac acgccaatca ggcgagaatt accaacatcg attttaataa tcccgatgat
     6661 tgcctgttca gcaaagacct aatcagtcat gcccgtgaac aaggcctctt tacgggcaag
     6721 gacgaagaat tcagcttttg cgacgtatat gcgccggccg attttggggc tttgcgttat
     6781 tgcgaagccc gggtttgggc ctatttcaac cgttttgccc ccggcatgga caaatacctg
     6841 aattacgcca tgggctacga tctcaacaat aaaatgcctt tgtcggtaga gcccacgcga
     6901 aaactgactg tgaaagaagt ggccgacatg atgcgcgatc attacgaagg cactcccatg
     6961 gacatgaccc tcgacatcgg agccggcgga ggtgcctgcc cataccgttg gaggcctatg
     7021 gagttcaaag tagacagtct gacttatctg aacgaaagag ccatagccac ccaacaaacc
     7081 ggttggtggt atgtggctca gtgtcgcggt tggttgcccc gcgatctggg cggtttgttc
     7141 tggtttgggg tggacgatgc cggcacctct cctttgatgc ccatctacag cgcttcgctc
     7201 gaagtgcccg aatgcgtacg ggagcacaac ggatctatgc tcgaatattc acctacttcg
     7261 atgttctggc tcaacaaccg gatagctcag tttgcctatt tacgctacga cgcgattgga
     7321 gctgaggtac gaagtatgat agataaacac gagcaggaac gcctgcgaga aatcttctac
     7381 gtcgatcaga aagccgccga attgtaccgt attaacccaa cgaccgccag ggaatacctg
     7441 acagactact ctttgcgtac ggctgccgcc ctgtttgaac gctggaagaa gttggacacc
     7501 tatttgctgg tcaaatatat ggacggcaac accaaaaaac aggaggagga cggcagtttc
     7561 aaaaacaacg gacacaggca ggacatgccg gcttcaccgg agtggggcgg ctacaatcaa
     7621 aaatggaaag aagccgtggt caaagatcac ggtgatgtgc tcagggttcc ctgaacaaaa
     7681 aaagacagta atatggaatg gtatcctttg gcctttgaac cgatcattaa aaaagtgctc
     7741 tggggaggtt cagccctggc cgaatttaaa caaatggatt tcgacagccg tcaagccggc
     7801 gaaagttggg agatttcggc agtgaaagac catgtgtcgg tactcgaaaa cggaccactc
     7861 agagggaaaa cgctcgaaga actcatccgt atcgatcccg agggcctgct ggggagcaag
     7921 gtcctcgaac gttacggcaa ggagtttcct ttgttgataa aatggataga cacggccgcc
     7981 gatctttcca ttcaggtaca tcccgacgat gcctttgccc gtgctcaggg acagcgtttt
     8041 ggcaagaccg aactttggta tgtggtacgt gccgggtctg agtccgggct gattgcgggc
     8101 ttcaacaaag agctgagccg cgaagaatac ctgaggcgta tcgccgacga cagcatcgaa
     8161 gaggttttac ttcggcatcc ggtgcgggcc ggcgatgttt ttatgatgcc tcccggcaga
     8221 gtccattcca taggtaaaga catcctgttg gccgaaatac aacaaagctc cgatatcact
     8281 taccggattt acgattttgg ccgcaaggac gaccggggaa ataccaggcc gctgcatacc
     8341 gagttggcgg ctcaatgttt ggattacaag ctttattccg agtacaagac aagctacccc
     8401 aagctattga accaggcggt tgaattaaat cgaaacaagt attttagagt taacaggttg
     8461 gaattaaaag cccaaacacc tctcctcgaa atagagcgca atcctgctgc cgggaaatcg
     8521 tttatagctt atatgtgcat cgagggagcc ttggagctgc attgttcgaa cggcagttac
     8581 gggcgtctga gccggggccg gaccttactc attccggcag cggcaggctc ttgccgcctg
     8641 caatgcaagg agtcggcgtt ggttttagag attgttgccg attgctgagt taaacttccc
     8701 gggaaagcgc aagaattttc cgaaaatgag ttaaacttcc cgagaaagcg taagatttcc
     8761 cgaaaacgag ctaaacttcc tgaaaaatcc aaactttccg aagcttagtc gaaaagattg
     8821 cgaaacttgg taaagatctt tttactcagg ctgggcgagg tcttgaagtt gtcggattgc
     8881 tccatacttt cgatcatcct ccgttcctcg cggctcaggt tctcgggaat atacacgctg
     8941 atgttgacca aaaggtcgcc cgtgccgtat tgattcaccg aaggcaagcc tttgcctctg
     9001 aggcgcagta tcttaccggg ctgtgtgccg ggatcgattt ttactttcac tttgccgtcg
     9061 acggtgggga tttcgacggt accgcccagg ctggccgtgg ggaaactcaa caaaagattg
     9121 tggataagat gattgccttc acggatcagt tccggatgat ctatggtttc gattactact
     9181 atcagatctc cgttgatgcc gccacggcgg gcagcgttgc ccttgccgct caaggaaagt
     9241 tgcatgccat cctccactcc ggccggaatt ttgatgttta ttatttcatc tttgctttct
     9301 atgccttcgc cgtgacaatg gctgcattta ttggttataa ttttaccttc gccctggcag
     9361 tcggggcaaa ccgacgatgt ttgcattcta cccagaatgg tgttggttac cctggttaca
     9421 ctgcctctgc cgttgcaggt gctacaggtg gaataggcag agccgccctc ggctccgctg
     9481 cctttgcaat gagcgcaggc aacctgcttt ttgactttga tctttttctc ggttccgtgg
     9541 gcgacttctt taagattgag tttaactttg attcgtaaat tggaaccccg attcactctg
     9601 ggttcgcgtg aaccgccaaa gccaccgaaa cccccgaagc cgcttccccc gaaactgctc
     9661 ccgccaaagc tgcttccgcc gaaatgacca ccaaaaatat cgccgaattg cgagaagatg
     9721 tcttccatcg taaaacctcc acccgaaaaa cctccaccgg cagctccttc tacgccggca
     9781 tgcccgaact ggtcgtatcg ttgccttttt tcgggattgc tcaatacctc gtaagcctgg
     9841 gccgcttctt taaacatctc ttcggctttc ttatcgcccg gatttttgtc gggatggtat
     9901 tgaatggctt tttgtcggta ggcttttttt atttcttctt cggtggcatt tctcgacacc
     9961 tccagtacct cataataatc tcttttcgcc atggtaaaat cttcgttttc ttaggtccgc
    10021 accatgaatc agtttgccac aactactttg gcaaaacgta tcactttgtc gtgcaggaaa
    10081 tagccctttt gaatgcactc aaccactttt ccgcgttgtt tttcgtctgt ggcaggaatg
    10141 gtcgtaatgg cctcgtgtaa atcggtgtcg aagtcttgat ctttagtttc aatggctacc
    10201 acaccgtgtt gtttcagata ggcagtcagt tttttgtaaa tcaattccat gccttctttg
    10261 aaagcagtta tttcgctgtt atcctgaaca gccgccagtc cacgttcaaa atcgtcgacc
    10321 acgcttaata tccctaccaa aaccgattcg ccgcctgctt taataagctc ggctttttcg
    10381 cgcatgctgc gcttgcggta attttcgtat tcggccagca ggcgcaaatt ggattctttc
    10441 agctgattat actttgactc gagttcctgt attcgtttag cgttcgtttc tttgctgggt
    10501 tcttgttgct tgcttttgcc ttttttttgt tctgaattga ctttggtttc ccgggatttg
    10561 tcctccccgg cttgttcttt ttggaggatt tcttcctgcg tatgttcggt tttgggcttg
    10621 ttttgtttgt tcataatctt gataaataga aatctatgct tgccgtttat actgcaaata
    10681 tcttgcctga acctaattgc atggccgatt tgtcagttat gacggatgta tttcggtgat
    10741 cttgacaatc tgtcagtaag tgcagcatac caggttgcgg tcaccgtagg cgttgtccac
    10801 ccttgatacg ggaatttgaa atttattgcc ttctatccat ggcaggggat aagcagcccg
    10861 gctccggggg taggggtaag gccattcgtc ggccgttaac agatattcgg catgaggcga
    10921 attcaccagc aagttgttgt ctgccggata gttgccggta cggacttcct caatttcccg
    10981 gtaaatgctt tccatggcct gtacaaaacg atccaattct tcgaggcttt cactttcggt
    11041 gggttcgatc atcaacgtac cgtgtacggg gaaggagagg gtgggtgcgt ggaaaccgta
    11101 atccataagg cgcttggcaa tatcggtttc gctgatgccg gcttctttga aaggtctgca
    11161 gtcgagaatc agctcgtgag ccacaaagcc ttcggctcct gtataaagca cggagaaatg
    11221 cgatttcaga caagcggcca ggtaattggc attgagaatg gctatttggg ttgcccgttt
    11281 caggcctgct gttcccaaca tacaaatata ggcataggtg atgggcagca gcatggcgtt
    11341 gccgtaaggg gcagaagcta ccgaaatacc ctttgttttg tcccaggact ctgtgggcag
    11401 gaaatccgcc agatgggcgg cacaacaaat ggggcctgca cccggacctc cgcctccatg
    11461 gggcatggca aatgttttgt gcagattcag gtggcaaacg tcggctccga taaaaccggg
    11521 gctggtcagg ccaacctggg cattcatatt ggctccgtcc atataaacct gtccgccgta
    11581 ttgatgaacg atacggcaca ttttgcgaac gtccggttcg aaaataccgt gggtagaagg
    11641 ataagtaatc atgcaaccgg caagtccggc agcgttttcg cgggctttgt tttcccagtc
    11701 ttccaggtcg acgttaccgt tggcatcgca tttaacctga actatggaaa agcctgcctg
    11761 agcggcactg gcgggattgg ttccgtgagc cgaatcgggg ataaggatga tatttctgcc
    11821 ggcttgaccc ttggctttga gatattccct gatcaccctc agtccggtat attcgccggc
    11881 ggccccggaa ttgggttgca gagaacaggc tgcaaagcct gttactgcgc agagttgttg
    11941 cttgagcttt tcgatgatgc tgaggtaacc ggctgcttgt tctttgggca ccatgggatg
    12001 gattgcgccg aattccggcc ggctcagcgg caacatttcc gaagcggcat tgagcttcat
    12061 ggtgcaggaa cccagcggaa tcatggagtg taccagcgaa atatccttgc gttccagttg
    12121 tttgatgaag cgcatcatag ccgtttcgga atgataggaa ttgaaaacgg attgttgcag
    12181 tatcggctcc tggcgaagca ggctgtcgtc gagggcgcag atgctgttcc aattctcttc
    12241 ttcgacaggc acggccaagt tgccggcggc cgaggcaaag acttcgatca agtcgttcaa
    12301 agcccgattg tcgacggttt cgtccagact cagtcccaga gttttgccgt cggggtaata
    12361 ataaagattg attccggccg cctcggccac cttgcgaaaa gatttagtgc tgagaccttc
    12421 gggtaatttg attttcaagg tgtcgaaaaa ggagccgttt tcctgttcgt agccgtacac
    12481 ttccagcgca tcgttcaggt aggaagtcat accgtgtatg cgtgtggcaa tatcgcgcaa
    12541 acctttggga ccgtgataaa tggcatagaa ggccgacata atggccatca gggcggtggc
    12601 ggtgcagata ttggaagtag ctttctcccg tttgatatgt tgttcgcggg tttgcagggc
    12661 caaacggtag gctgttttac cccggctgtc tctggatagt ccgacaatac ggcccggcat
    12721 ttgtcgtttg aactcttcgc gggcggcaaa gtaggcggct gccggccctc cgtagcccat
    12781 ggggattcca aaacgctggg aagagccaaa tacgatgtcg gctccccatt tacccggggc
    12841 ttccagtatg accagagcca tcaggtcggc tgccacggcc agtttgagcc cggctttatg
    12901 cactttatct gcaaagcttc ggtaattttc taccttacct tcggcattgg gatattgcag
    12961 cagggctcca aaaaaactat catcgggcct gaaagcggcg taatcgccca ggaccagttc
    13021 aataccttgt tccgaacagc gggttttgag aactgccagt gattgggcaa acagtccccg
    13081 gtcgacgaaa agtttgttgg ctcccgatct ttcctgttcc cggctgcgca gattgaacat
    13141 catgtgagcg gcctcggcag ccgaagtggc ttcgtcgagc agagaactgt tcgaaagcgg
    13201 caatccgctg agctcacaaa ccagggtttg gaaatgcagc agagcttcca gccggccttg
    13261 cgaaatttcg gcctgatagg gagtgtaaga agtgtaccag ccggggtttt cgaaaacatt
    13321 gcgctgaata acggccggtg tgatgctgcc gtaccagcct tgtccgatgt aagagcggaa
    13381 cagcttgttt tgggcagcca gcccggcaat gtgttccgca aaacgggctt ccgacatcgg
    13441 ttcgattggt tcgggttcct gagtgaggcg gatatcccgg ggtaaggttt tgtctatcag
    13501 ttcttcgaga ctgttcaagc caattttggc gagcattttt tgttctgctt cggggctgat
    13561 gccgatgtgg cgttctgaga aaaggagagt gttcatatta ttgattataa gtttgttaaa
    13621 tcgatgattt cttcttcgag caagctgatt ttttcgccct gattcttgcc gacaacaaag
    13681 gtgttttcaa tgcccacggc tccgacgcct tcgacgacaa atttaggctc tatggcaagc
    13741 accatgcctg cttccaaaat atctttcatt ttgggagcca acacaggcaa ttcgttgata
    13801 actaagccca ctccatgacc gacaaagccg gcctgttgat tcttgcccat aaaacaatct
    13861 gccaaaccct gagaagcgac aaagtctaag gaaaaaaagt ataaatcctc gcagatttcg
    13921 ccctctttca atctttcctg aaggcgatcc tgcagttcca gggcgacttg gtgggccttg
    13981 taggcttttt cgggaagttt cccataggag tagcagcggg aaagatcgtc cagataacct
    14041 gtgaagttgc cggccatatc gatcatgacg ctatgtccgg ggagtatggg gctgccgttg
    14101 ggtccgatgg ggttggaggg atgtccggct ccccccaggg caaaatcgta aggagagctt
    14161 accagggcgt tatcgccggt caatacagta cccataaaga catccatatc ggctccgtag
    14221 gttctgaaca aacccaggtt gccgttcagg cgcataatcc gttcgagttc tatggataat
    14281 tcaatgtccg tcatgccggg cctgaagagc cggggtatgc tccgatagga ggcagcgtgc
    14341 agttcggccg accggcgtat caaagactgt tcgtaaggcg tttttacgct gcgggctttt
    14401 cgcagagcct ggctgccgtt tatcagccga ctgctgggaa aacattgctg caaccttgtc
    14461 cattcggcgt agctcagttc atcgccttcg agcatcaggt tttcggcggg gggcattccc
    14521 agttctctga ggattccggg tatttgctcc actttgcgaa tcaggtgaaa atgtgcctga
    14581 tccggttgtg acccggggct tttttgtccg gtttcgccgg cagggaatcc gatgtcttcg
    14641 gctttcagat cgaggggacg gcgaacaaag tgaatcgcct cgccctcaca gggtaaatag
    14701 caatagcctt tatatattct gttgttgagg taatacaggt tgactgagct gcttaaaaga
    14761 caggcatcgg cttgcaggga ttggagttgt ttcaggatat gttgacgcct gagagtgcct
    14821 tcgttaatca tcagaataat aacattttat atttttttaa cccgcttgtg caaggagggc
    14881 tttttaaaac gtcaaaggta ggcattaaaa aaaataattc ctacctttgt accctttttc
    14941 aaaatcaccg aaaggaagct gttcaaccga catatgcctc aacaacgata catcgagatt
    15001 cagggagctc gtgtcaataa tcttaagaat atcagtgttc agattcccga aaacgctttt
    15061 actgttataa ccggtttgtc gggttcgggc aaatcctctc tcgctttcga cactctatat
    15121 gccgaaggcc agcgtcgtta cgtcgaaagc ctgtcggcct atgcccgtca gtttttaggc
    15181 agaatgaaca aaccggaggt ggattttatt aaaaacatac ctcctgccat cgccattgaa
    15241 caaaaggtaa atacgcgcaa tccccgctcg acggtgggca cttcgaccga aatttacgaa
    15301 tacctcaggt tgctgtttgc ccggataggt aaaacttatt caccgctatc gggcgatgag
    15361 gtcaaaaaac accgaaccaa agacgttatc gagtacgtgc tgaaatttcc cgaaggcagc
    15421 cgctttttgg tgctctgccc gctcaatctg cccaaaggac gaagcctcga agatcatttg
    15481 gaaagcttta taaaacaagg tttcagtagg atagagtatc gggggcagat actaagtata
    15541 gaagattttt tgtcccaagc cggtaagata agcccgtccg gtattgaaag cctctacctg
    15601 gtcatagacc gcttgttggt gcaaaaggac gacaaattgg tacagagccg tctggccgat
    15661 tccatcgaaa ccgcttttta cgaaggagat cacaattgtc tgattcggat gtttaccaat
    15721 gaaggatcgg agctccggca tttttccgat cgtttcgagg cagacggaat ggtatttgaa
    15781 gagccgaacg acctgatgtt cagctttaac tctcccgccg gagcttgtcc tgtttgtgag
    15841 ggcttcggta aagttctcgg cttggatccc gacctgatag tgcccaacaa atccctgtct
    15901 gtttacgacg atgccattgt ttgctggaga gggcccaaaa tgagcgaatg gaaaaatcgc
    15961 ctcgtggaga atgccgccgc tttcgacttt ccggtgttta agccctggta tcagctcagc
    16021 gaatcacaaa agcgcctggt ctggacaggg aacgagtatt ttcagggcct tgacgatttt
    16081 ttccgtatgg tggaatccaa tcagtataaa gtgcagtacc gggtaatgct ttcacgctat
    16141 aggggcaaaa ccatctgtcc ggagtgcaaa ggcaccaggc tcaaaaaaga agcttcctac
    16201 gtaaaggtcg gcgggcgaag tatttcggaa ttggtcgatt tatctatact ggaactgaac
    16261 tctttttttg aacaacttca actgagcgcc aacgaaaagc tcatagccga acgcttactt
    16321 accgaaatac ggacccggat caaattcctg ctggatgtag gattgtctta cctgagtctc
    16381 aaccgcttgt cttcttcgct ttcgggcggt gaaagccagc gtatcaactt ggtcacctcg
    16441 ttgggtagca gcttagtagg ttcactgtat attttggatg aaccgagtat aggccttcac
    16501 gccagagata cggctttgct gatcaaagtg ctcaaagaac ttaaagagtt gggcaacacg
    16561 gtagtagtag tagaacacga cgaagaaatt attcgggcgg ccgattacat tatcgatatt
    16621 ggccccaaag ccggccgttt gggaggagag ttgatgtatc aggggtcggt aagtggcctg
    16681 atcaaccatt ccgagtcggt atcagtgaac aagcaggaga aaacaagccg gaagaagccg
    16741 gtcaaagaga accccgtcca atttcaaagt cataccctga attatttgag cggaaaggaa
    16801 aaaatacctt tgcccgagtt gagacgtccc tggaacaatt acattgaaat cgttgcagcc
    16861 tgccatcaca atctgaaaaa catcaacgtc aagtttccgt tgaatgtgat gacggtggtc
    16921 agcggagtga gcggctcggg aaaatcctca ctggttcggg atatttttta tgtggcttta
    16981 aagaggattt gtagtgagac caccgatagt tgcggttctt tcggcgaact cagaggcgat
    17041 atccatctgg tacataacat tgaatttgtc gatcagaatc ccataggcag atcgtctcgc
    17101 tccaacccgg tgacttacct caaggcctac gacgagattc gtaaactctt tgccgaacaa
    17161 gccctggcaa aacaaatggg tttcaacgca tcttactttt ctttcaatac cgacggcggg
    17221 cgttgtgaag cttgtcaagg cgaaggagtg gaaagagtgg aaatgcagtt tatggccgat
    17281 ctggaaatgg tttgtgagca ttgtcacggc aaacgtttca agagcgatgt gttggaagta
    17341 ctttaccgcg aaaaatccat tcacgatgta ctcgaaatga cggtggacga ggctatagag
    17401 ttttttggac agggcaggca agccctcgaa aagaaaattg tcaaacgcct gctgcccttg
    17461 caggaagtag gcttgggtta catcaagctg ggacagtcct cttctaccct gtcgggcggt
    17521 gaaagccaaa gggttaaact ggcctttttt ctaagcaacg aacgcgaaga gcccggagta
    17581 tatgtttttg acgagcctac tacaggccta cattttcacg atattcatat tttgcttaaa
    17641 gccctgaatg ctctgattgc caaggggcat accgtgatca ttattgagca caatgtggag
    17701 attattaaaa gtgccgatta tgtgatcgac ctgggccccg aaggtggaga ccaaggagga
    17761 tacctcttat ttcaggggaa acccgaagat cttttgcaat gtaaagagag ttatacggcc
    17821 agggccctga aaaaacactt gaaaggctaa acattcccaa aatcatcaac caataaaccg
    17881 gccaacaatc aatctgaaat ttcaatcaat agtataaaat taaaccgtat ttaaagaagt
    17941 gtaattatgg actcaattgc agtagattca acagcaaaaa gcagtttacc gtttgagccg
    18001 gcagtcgtga cggaaaccgt tactcaaaca gtagaatcga tgaatcagtt ttggcataaa
    18061 gcccgggact tttttaccga tgttttactg gccaacgttc ccagaattat cattgccctc
    18121 gtattgcttt gggtaggatt caggctggtg aattacctta ccagaatggt tcgcaaaatt
    18181 atgcaggccc gtgagatgga taagtccttg caggattttt tgggctccct aatcggtata
    18241 atactcaagg tgctgataat aatgagtgtt atgcagatga taggcgtggc ggtaacttca
    18301 tttattgcca ttctcggtgc cgccggcctg gccattggta tggctttgca gggcaccctg
    18361 caaaactttg ccggtggggt tataattttg cttttaaaac cctttaaagt gggtgatttt
    18421 attgagcaag gttccatcag cggaacggtt acgagtatcc agatttttaa tactgttttg
    18481 agtactcccg acaacaaagt cgtgattgta cccaattctc aattagccac caactccctg
    18541 atcaattaca ccaaatccga agtccgccgg gtggatgtaa gcgtaggtat cagatacgat
    18601 gaatccatta ataaagtccg caagttgtta ctcgatttgg cttcggctta tcccgaagtc
    18661 ttgaaggagc ccgctgttcc cgttgtacac gtgacatcga tgggcgaaag ctcagtaaat
    18721 cttcagttaa ggctctgggc aaaaaccgaa gattactggc ccctgtactt ttcgcttaat
    18781 caggcagttt tggatgagtt tagtaaacag gatatcaaaa cacctttcaa ccaactgtct
    18841 gttcgtttcg aagaaggtgt ttctccaacc aaataatgaa tgtaattatg aaatcgaaat
    18901 ttttattatt ggctttgact gccggtctgc tgtttagttt tgctcccgaa cggaaaatag
    18961 tcgtatatac catcggtgat tccaccatgg caaacaaacc acttgacaat atgtctctgg
    19021 aacggggttg gggacaggtg ctctctgcct acctcgacag ccgttacgta gaagtcgaca
    19081 atcacgcccg caacggacgc agcagcttga gtttcagaaa cgaaggcctt tgggctccca
    19141 tttacgacaa aatcaaaccg ggtgattacg ttttgataca attcggacac aacgacgaaa
    19201 aaccctcgcc cgaccggcat accgaaccgg gcagcacttt caacgaacaa ttgcgccgct
    19261 atgtgttgga aacccgcgaa aagggaggta ctcccgtctt gcttactccc atcgtaagac
    19321 gtaaattcga cgaggaaggc cgattgattc ccacccatgg cgactatccc caggccgtgc
    19381 gcgatgtggc tgccgagttg gacgtaatcc tgatcgatca caacgttttg agccgccatt
    19441 tggttcagga tctgggcccc gaagcatcga aagaattgtt tatgtgggta ccgcccggta
    19501 ccaatccggc tcttccccag ggcaaagaag acgataccca tttgcgtgct ttgggcgcta
    19561 aacgaatagc tcgcctggtg ttgaacgaga tcaaagaaaa aattcccgct ttggccgttc
    19621 acgtcagaga atacgatttt gtggtggcca aggacggctc gggcgacttc ttcaccgtac
    19681 aggaagccat caatgccgta cccgattttc gcaaaaacgg acggacgact atttttattc
    19741 gtaacggagt ttacaaggaa aagctgattt tgcccgaaag taagatcaat gtcagtttca
    19801 taggcgagag cgttgagcac accattttaa cttacgacga ctacgctcag aaattgaata
    19861 ttttcggtga atcaaaatcc acctccggtt cctcgagctt ttttatgtat gctcctgatt
    19921 tttgggccga gaacatcacc ttccaaaatt cggccggtcc cgtaggtcag gctgttgcca
    19981 tttttgtcag cggcgacagg gccgtgttca agaactgccg ttttttgggc tttcaggata
    20041 cgctctatac ctacggcaaa cacagccgtc agtactatga aaattgttac atcgagggta
    20101 cggtcgattt tatattcggt tcttcgaccg ctgtgtttaa agattgcgaa atcagatcca
    20161 agcgcgacgc ttatatcact gcggcctcta cccctcaggg ccgggattac ggctacgttt
    20221 tcatcaactg caaactcacg gccgatccgg gagtggagaa agtcttttta ggcagaccct
    20281 ggcgcccgta cgcccaaacc gtgtttatcc actgcgaaat gggtgctcat attcgccccg
    20341 aaggctggaa caattgggga aacgtttcca acgaaaagac agccttttac gctgaatacg
    20401 gctcttcggg ccccggagcg gacatatccg gacgggtacg ttgggccaaa aaactcaaca
    20461 agaaaaaact ggccgcctac agcatagaga aggtgctggc cggcgacgac ggctggaatc
    20521 ccctttagcg ttttttgaaa ctaaagccgc tattcttaaa attctttttg aaaaaaagca
    20581 ctgaaaaaca gatagccacc accaaaccga tgccaacaga gaggtaattg ttcagcctga
    20641 agccttcggg cgaagtcaga atataagtgg tgcaaacgac ggtcataaac agagccggca
    20701 acagggcaat aaagtaattt ttgccttccc ggaacagata aacgcttatt gcccacaaag
    20761 taaatacggc cagactttgg ttacaccagg caaaatacct ccaaataatt tcaaaatcaa
    20821 tttgaagtaa gacaaaggca gcggcaaaca aggggaaact cagcaggatg cgtttgtaca
    20881 ttttcttttg atcgtaatgt aacagatccg acataataag tcttcccgaa cgaaaagccg
    20941 tatcggccga agtgatgggg gcagctacaa cgccaagcag agcgagtacg cctcctactt
    21001 tgcccagcca gccccgtgaa atagtatcta cggcaatggc ggccgaattt ccgttttccg
    21061 acataaagag ctgaaactta tccagtcctc cgaaaaatgc catacctgca gcagcccaga
    21121 tcaatgcgac gcaaccttcg gctaccatgg ctccgtaaaa tactttcctg ccgtcgcatt
    21181 cgtttttcat gcagcgggcc atcaggggag attgagtggc gtgaaaaccc gaaatggccc
    21241 cgcaggctac tgtgataaac atcatgggaa aaatgggtaa acgagctgct tcggggtgca
    21301 ggttgcgcaa gcctgcccgg ttgagttcgg gcagattgtc caggttcggg ataagagtca
    21361 ccagaatgcc cacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccacta
    21481 actcgggcag gctctcgcca ttgtaacgct cggaaatcat ccccgaaaag taatcgtgca
    21541 accccccggc aaagattgtt cccagtgtta tccataggaa gaccacagga ccgtacatgg
    21601 ctcccataac ggctccaaaa ataggcccca gaccggcaat atttaaaaat tgaatcagaa
    21661 agattttcca tgttggcatg ggaacgtagt cgactccgtc tcgtaggctg aaggcgggtg
    21721 ttgtgcgggt tttttcgggt tggaaaactt tctcaagata ctttccgtat aaaaaatagc
    21781 cggcaatcaa agccgtaaga caggcaacaa aaccgatcat gataaaattt taagactatg
    21841 ccgtaacagg caatcgcaaa agtacattga attttattat ctttgcggac tatttaatac
    21901 aaaaagcgat gaaaaagaag aaatttttaa cttgtgacgg gaaccaagct gccgcacaca
    21961 tatcgtatat gttcagtgaa gtagctgcca tttatccgat cactccttcg tccactatgg
    22021 cagaatacgt agatgaatgg gcggctgccg gacgcaagaa tatgtttggt gaaacagttt
    22081 tggtacagga aatgcaatcg gaagccggtg cagcgggggc ggtgcacgga tccttgcagg
    22141 ccggtgcgct gaccagtacg tatactgctt cgcagggttt gctgctgatg attcccaata
    22201 tgtacaagat tgccggggaa ttactgcctt gtgtgtttca cgtttccgcc aggactttgg
    22261 cttcgcatgc actgtcgatt ttcggagatc atcaggacgt tatggcttgc cgtcagaccg
    22321 gttttgccat gctggccgaa ggctctgttc aggaagttat ggacttatcg gctgttgccc
    22381 atctggcttc cattaaaagc cgggtgcctt tcgtgaactt cttcgatggc ttccgtactt
    22441 cgcacgaagt gcagaaaatc gagatgctcg ataccgagga tctgtctcct ttggtcgacc
    22501 gggaagcgct ggctgctttc agggctcgct ccatgaatcc cgataaaccc gtaatgcggg
    22561 gaatggccga aaaccccgat catttcttcc agcaccggga agcgctcaat cctttttatg
    22621 aggcagtgcc cgatattgtt gccggttata tggaggaaat caacaagttg accggccgtg
    22681 aatacaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc aagacggtca
    22801 aaagaattgc cgtactggac agaaccaaag aacccggagc caatggcgaa ccgctgtatc
    22861 tcgacgtagt cgaagccttt gcaaaaaacg atgcattggg tctggacaaa atgcctttca
    22921 ttgttggcgg ccgctacggt ttgtcttcga aagacaccac gcctgcccag attttggccg
    22981 tattcgaaaa tctttcctta cccgaacccc gcaaccagtt taccataggt atagttgacg
    23041 acgttacgtt cacttctttg cctcaggagg aagaaattgc actgagcggc gaaagcattt
    23101 acgaagccaa attctatggc ctgggcgctg acggtacggt aggagccaat aagaattcca
    23161 tcaagattat cggagacaat accgataaat attgtcaggc ttatttttcg tacgactcca
    23221 aaaagtccgg cggttttacc tgttctcacc tgcgttttgg cgataccccc atacgttcga
    23281 cctatttggt gaatacgccc aattttgtag cttgtcatgt gcaggcttat ctgaaccttt
    23341 acgatgtcac caaaggtttg cgtcagaacg gtactttcct gctgaacacc atctgggaag
    23401 gcgaagagtt ggccaagcat ttacccgcca aggtcaaacg ttattttgcc aaaaggaaca
    23461 tcagtgttta ttatatcaac gccacccgga tagcacagga gataggcctc ggcaatcgta
    23521 ccaacaccat attgcagtcg gctttcttca gaataaccaa ggttatagat gtggatctgg
    23581 ctattgatca aatgaagaaa ttcattgtca agtcctacgg taataaaggc gaagagattg
    23641 tcaggaaaaa ctacgaagcc gtcgacagag gtggtgaata caaacaactc aaagttgatc
    23701 ctgcctgggc taatctgcct gacgaccccg cggtaatcaa caacgatccc gaatttatca
    23761 acaaagtagt tcgtcccatc aatgcacagg acggcgactt gcttccggtt tcggccttca
    23821 aaggcatcga agacggcacc tggatgccgg gaacagcccg gtacgaaaag agaggtgtca
    23881 gcgcctttgt acctgaatgg atagccgaaa attgcattca gtgcaataaa tgtgcttacg
    23941 tctgtccgca tgcctccatt cgtccttttg tactggatga ggcagaggct gccgcagctc
    24001 ccgggttcga aaccatcgaa atgaaggctc ctgccaccat gaagggtatg caattccgta
    24061 tacaggtttc ggtgttggat tgtttgggtt gcggcaattg cgcggatgtt tgtccgggca
    24121 accccagagc cggaggcaaa gcccttaaga tggtagacct cgaaagccag atgagtgaag
    24181 cttccaattg ggaatattgt gtagacaaag tcaaaagcaa acaacacctg gtcgacacga
    24241 aacaaaacgt caagaattca caatttgcca ctcccttgtt tgagttttcg ggagcctgtt
    24301 cgggttgcgg cgaaacgcct tacctcaaat tgttgaccca gttgtttggc gaccgccaga
    24361 tagtggccaa tgccaccggc tgtacttcca tttattcggg ctcggtacct tccacgccct
    24421 acaccaccaa cgagcttggt cacggtcctg cctgggccaa ctcgctgttc gaagatttct
    24481 gcgaatacgg tttcggtatg caactggctt acgaaaagat gcgtgaacgc ctggttaagc
    24541 ttatgagcga tgctcaatcc tgcagccgtt gttccgaaga acaaaaagcc ttgtttgccg
    24601 aatggctcac cgatcgtgaa gacggcgaca agagccgtgt actggccgac aagatcatag
    24661 atgcggtaaa aggatgcgac tgtgattatt gtaagcaaat cagcagccta agcaagtatc
    24721 tggtaaaacg cagtcaatgg atcatcggag gagacggtgc tgcttacgac atcggttacg
    24781 gaggattgga ccacgtgatc gcctcgggca agaacgttaa tatcctggtg ctggacactg
    24841 aggtgtattc caacaccgga ggccagtcgt ccaaagctac tccgttggga gcgattgcca
    24901 aatttgccgc cgccggcaag cgtatacgca agaaagattt gggaatgatt gccaccacct
    24961 atggttatgt atacgttgct caggtagcca tgggagccga tcaggctcaa accctcagag
    25021 ctttccgcga agcggaagcc tacgacggtc cttcgttgat aattgcttac tcgccttgta
    25081 ttaatcacgg actaaagagg ggtatgggta aatcgcaggc cgaagccgaa gctgcagtag
    25141 cttgcggtta ctggcatctg tggcgctaca atccacaact cgaggccgag ggtaaaaatc
    25201 cctttatact cgattccaaa gaacccgact ggagtaaatt tgccgacttc ctcaaaggcg
    25261 aagtccgtta ttcatcggtg ataaagcaat atccggatga ggccgaagag ttgtttaaag
    25321 ctgccgaaga aagcgccaaa tggcgctata acagctataa acgcatggcc gggcttaact
    25381 gggaataaat ttcggatttc cgattcatca tcggattccg gtctaagaaa cgaaggctcc
    25441 gcttttgcgg ggccttcgtt ttgtatgagc taaacagggc tattttgtcg aacgattttt
    25501 ttaccttcgt cgaggtcttc attgtctgag ctatacagag gagcgttttc tgccgtagag
    25561 ttatttgtta tctgtttttg aacgggtaaa aggcacaaaa cggacggcca gcttgcggct
    25621 gtaagtaaat tggcctgcct ttttttcgat cagtaccagg tattgcgtgt ctccttcggg
    25681 ccctaaaggt aaaatcatac gtccgttttc ggccagctga tctagcagat ccggaggtgt
    25741 ttcagctatg gcggccgtaa cgactatggc atcgaaaggt gccatttctt gccagccacg
    25801 gtaaccgtct gccgttttgt aacgaatgtt tttataaccc agtgtgtcta agacattttt
    25861 ggctttgatc cccagttccg gaatgatttc gatggtatat acttcccggg ccagttcggc
    25921 caggatcgct gtctggtaac ccgatccggt gcctatctca agtattttgt cgcaatcgcc
    25981 tgctttgacc gaggctgtca tataggctac gatatagggt tgagatatcg tctgacccag
    26041 gccgatgggc agggctgtgt cgctgtaggc atgcgattgc agcttctccg gaacaaagaa
    26101 ttttctgtcg acagcctcca ttgcccggag ggttttggga tcgtttatct cccttaaaaa
    26161 gagcttattc atgactctta aagaattaaa tatcaatcaa aattaaaaaa ataaaagaca
    26221 aaagtcaaaa acatactata tttgcatgtt tgctttcata attttatttg tttcgtcgat
    26281 gaccatcaat ccttttagta tcgcttacat cgcctcggct cttataaccg tgggcctgat
    26341 ggtttttctt ttcgttaaaa ggaaagagag ccttagcact tatgtttaca tgttgttgac
    26401 ttgtactgcc gtgtggtcgt ttttttacgg cctggaactg ggctcgtcca acagcgaaga
    26461 cattaggttc tatgtgcaga tagagtatgc cggcatctgt ttgttgccga ttttttacct
    26521 gttttatata cttgaatata ccgggagaga aaaacacctg aatacatcgc tttacccttt
    26581 attttttata ataccgcttg ttactatttc catgttgatg agcaacgatg ttcatcatct
    26641 tttttatgct cagtcttcgt tcaaaaccgc aggtcgctac tattaccatg cattagttcc
    26701 cggacctttt tactaccttc atgtggttta ttcctatggc ctgatagttt taggtatagt
    26761 attgttgctt aagtcataca ggggacagag caaaagacaa cgcaataata ttttgatttt
    26821 actctccggc acaataattc ctttccttac cagtattgcc tatatacttg gtttcaaata
    26881 cgcgggtttt atcgatttta cccccctggg ttttctcgta atgtctataa tagtggccta
    26941 tgctgcctct accaataggc tatttgaaat gactcctctg gctatgagtt atttatacga
    27001 gatcattccg gacgccatct ttgtgttgga ccgggattca gctataataa ataccaatcc
    27061 cagcgcccaa aaattattaa agtccggcat catggaggaa tgccccgaaa gcgaaaaatg
    27121 tttgcatttt gaagaattct ccgatgtgga ggaaataaaa tacaaagaaa taaaagcggg
    27181 cgataagcat ttttttatta ccaaaaaggc agttgtagga ggaaacaacc agcaaatcgg
    27241 cctgatgtta atagtgaagg atgttacgga agtaaaaaat ctcgagcata tgcaggaaat
    27301 cctgatgtcg attgctctga attttatcaa tacccctctg gacaattacg aaaagacggt
    27361 taaagcgtcg ttggaacaaa tggggcgtta tgtaaatgct gaaagggttt gtatagcaag
    27421 ttttaactac ggtaagggtg ttgttgatct tatccatgaa tggcgtagac cgggagtcga
    27481 ttctattatt gacgagatcc ggataataac tcttgaggat atcaaagaac tgatagagat
    27541 tcataaaagg cgggagagtt tttttgtaga agacgccaag gtattgatcg aagggactcc
    27601 cttgtatgat ttgctcaatt taaaaaaaga aagaagtttt ttggccattc ccatgtttga
    27661 ggcttccaat ctgattggtt ttttgggttt caactggtcg tataaaaatc agaggtatag
    27721 ttttgaggaa caacaattgt tgcagttgtt ttccgaaatt ctggtgaatc tgatcagccg
    27781 ggaggaagcc aacattatta tcaaacaaaa gtcggagata gagaagttta catctgaaat
    27841 tttctccgat tttatacagg tcaatattca aaatattcag gaggttgccc ctaaaaccct
    27901 cgaaaaactg ggccgttttc tgcaggtgga aggtgccttt ttaatccaat tcggaacgga
    27961 tcggaatctt cagatcttta aatggagcct gattccgagg agtgaacctc aacaaatagc
    28021 tctagaagac catgcttggt ttagcaagca agtcaataaa ggggtactca gcatcgataa
    28081 tctggaagat ttgcctgacg aagccgaatt ggaaaaaaac gaccttaaag ccttgggtgt
    28141 caaatctatg attgctgttg ctttgagaaa caaaaaccaa accatcggag taataggttt
    28201 tcaaatgttc ggcagttacc gccggtggac cggcgcagat gccgatatgt tgataagttt
    28261 cagcaatttc atggccgagg ttctctttaa ggtcgagcgc gaaaaagagc tcatacaagc
    28321 cagaaatctg gccgaagcgg ctagtaaagc aaaatcggaa ttcctttcca ataccagcca
    28381 cgagatacgt acccctctga acggcatgat aggttttacg gaattgttgc gcaacaccaa
    28441 gctaaacaag atacaattgg aatatgtgga aaacgccatc atgtcggcca acaccttgat
    28501 gggtgtgata aacgatattc tggatttttc caagattgaa tcgggtaagt tggaactcga
    28561 catcgtgcac agcgatatta tacaattggt agaaaaatcc tcggacatag taaaaattat
    28621 ggccagtcaa aagggcctgg agcttttact caacatacaa cccgatgcgc ccaggtatgc
    28681 ctatatcgat cctattcgcc ttaagcagat tatggtaaat ttgttgagca atgccgttaa
    28741 atttacgacc gaaggcgaag tggaaatttg tctaaaattt aaggaaatcg acgaatttga
    28801 gggacaattc accttggctg tgcgcgatac cggcataggt atccgcgacg aagataaaag
    28861 taaactcttt aaagcctttt cgcaggccga ttcttccgtt acacgtaaat acgggggtac
    28921 cggccttgga cttgttatat caaattctct ggccgaaaaa atgggcagca aaatcgattt
    28981 cacaagcgaa tacggcaaag gttccgtttt ttcgatgagc tttgtaagcc gctacgaacg
    29041 taagcaagca gaagaaagca tgggcagcct cccggtaaaa agggtgctga ttgttgacga
    29101 taacgccaac aatcgcaaaa tcctggaaca taatttcatg tattgggggc tggatttcag
    29161 tagcgcagga agcggcaaag aggccttggc tgtgctcgaa aaggattcgg atttcgattt
    29221 gatcctgatc gattataata tgcccgaaat ggatggtcta acggcagttg ctgccataag
    29281 ggagaaaaaa cttctgaatc ccgaaaagca ggccattatt ttgttgcaca gctcctcgga
    29341 agatcacacc ataaacgaaa gagcccgtaa attacaaatc tggcgaacag tcattaagcc
    29401 acttaaggcc agtgacttgc tcttttctct aaagaatttg tccccggtaa gtccggcgac
    29461 agatcttgta aaagctccgg aaagtccact cgttcgggaa aagccggttg atttttcaaa
    29521 acaattttgt gtattggtgg ctgaagatgt caagatgaat atgatgttgg tggttcagct
    29581 tataaggaaa aggtttccca atgccaagat actggaagcc gaaaacggca aagaagtgct
    29641 cgaactgctc gaaaataatc tcccggacct gatctttatg gacgtgcaaa tgcctgtaat
    29701 ggacggtttg gacgccagtc gcaatatccg tacccacaac aatccccgtg taagaagctt
    29761 gcctgttgta gcccttaccg ccggagtttc caaatcggaa caggagttgt gcaaactggc
    29821 ggggatgaac gattttattc ccaaacccat tgataaaaag atcctgtatc ggatcatatc
    29881 gaagtacgta gcctctgcaa gcggaaaatg gcaggccgaa gtcgaagaag gcgaaaagcc
    29941 ttcagatatt tctttgcatt tcaacaaaaa caatctcttg gacaaaatcc agaacgacaa
    30001 agatttgtat aaacaattaa tgtttcaatc tttgaaggag ttcgacaaag atgtaaatga
    30061 attattggaa aatatagaaa ccggagatcc ggaccggata aaatcgggag cccacaggct
    30121 caaaggcagt gccttcaata tggagtttac gcgcctgggc aatttagcca aggtagtgga
    30181 acaaaatgct tccgacgttt ttttattaga gtccgagggc gaaaaactaa aaacagaatg
    30241 gcttacccta aaagatataa ttgcaaacga aatataagta accatgaaaa catctcaaac
    30301 tgaaacggag caaaggctaa gcatacgtcg tacttctcat aaaaaccgtt tagtgattat
    30361 tggcatagtc tgttctttta ttttgtttgt tttttatgca ttcaattcct ggagagcggc
    30421 cgataaggat ttagaaaaag gagctttggc ctatgctcaa accatcgaag tggcgttaaa
    30481 cacagtaaga ctggaatttc tgcaggccaa tgagggagta gaaagctcgg attactatcg
    30541 gattattaag gataaactga ttgaactccg cagaatgaac ccgaatacgc gtaccattta
    30601 tttattgaaa caagaggaag gtgaggttat ttttttagtc gattcggaac ctgccaattc
    30661 gatatactat tcttcgcccg gacgggagta ttacgatacc gatacggcca ttttaaaagc
    30721 tttcgaccgg gctgagccgg taattaccga ccaccacaca gatagtttag gtaaatggaa
    30781 aagcgtggtg gtgccattga gaagtttcag aaattcggct gtgtatgcct tattgggtat
    30841 tgattacgat gtcaaccgtt ggaaaaaatt ggttttagcc gaagcctcag aagatatcat
    30901 ctacgctttt gctgttgtat tgctgtttgt cagtctttta ttgataatga gtaaaagcaa
    30961 ttctctgaac aagcaggaaa aattacgcca aatagctgaa caacaagcta tggccttgct
    31021 tcatgttgta gaacaaagcg acgatattat cacggtaaaa gactcagatc tgagggtatt
    31081 tgctgccaac aaggctttcg cccgctctgc cggctacgac gattttaccg aggttatagg
    31141 taaaaccgat gcggaaattt acaatattcc gccctgggaa gaacctgtca gaacctatat
    31201 ggacgatgaa cgaagagccc gtagtttgaa gccgggcgaa tatttactgc gcgaagagga
    31261 attgatcact catacaggcg aaagaaggac catcctgacc aagaaatacc ccatttacga
    31321 cacaagcggc aatgtcttgt ttaccagtaa tatttcgcga gatataaccg aacggaaaaa
    31381 gatggagata gagttgaaaa attccaaaga actcgcagaa tctgccagca gggcaaaatc
    31441 ggaatttctc tccaatatga gccacgaaat ccgtacccct ctcaatggtg tgataggttt
    31501 taccgaattg ttatcgaaca cgcctctgaa taagatgcaa aaagaatacc tcgacaacgc
    31561 tatagtatcg gccaattctt tgctgggtat tataagcgac gtactggatt tctcaaagat
    31621 cgaggccggc aaacttgaac tcgacatggt atcgaccgat gtcatccgtt tggtagagag
    31681 cgccgccgac ataatcaaaa tacaggcttc gcacaaggat attgaattat tgctcgatgt
    31741 gcagcccgac ttaccccgtt tcatgacact cgatcccttg cgttttaaac aggttttagt
    31801 taacctgatg aacaatgccg taaaattcac tcatgcaggc gaagtagaat taaagctgaa
    31861 ttttatacgc cttgacgaaa acaaaggaca attgaccgtg agcgtgcgcg ataccggcat
    31921 aggcataaaa gaggcagata aaagcaagct gttcaaagcc ttttcacaag ccgacacttc
    31981 tacaacacgc agatacggag ggaccgggct gggtctgatt atatccaatt ccatagtgaa
    32041 gaaaatggga ggagaaattc agtttgaaag caaaatagat gaaggatcca ggttttattt
    32101 cacgctggaa ctcaattacg acgacgacac taccgatgtt ctaagcccga taaaagatat
    32161 caagacggtt ttgtttgtag acgataatga gaataaccgt cttatcctgg aacgaacttt
    32221 tgagtattgg ggtattgaat ttaccggttg cgatagcgga ttgaaagcga taaaattgtt
    32281 acagtcggga aaacatttcg atttgcttat cgtagattat catatgcctc agctagatgg
    32341 tttggaaacg attaaattgc tgcgaaaaga cgaacgtttt gctgccggta agttgcctgt
    32401 aattctttta catagctctt ccgaacacga caccatacat tgcgccgcca aggaattgaa
    32461 tattctgtac acccttacca aaccggttaa ggcggttgaa ctttattatt acctgcagaa
    32521 acttcaggcc ggcaatgggg atacttacga aaagtattta caaaagttgt ccagaaaaga
    32581 agaatccagt gttcgcatac tcgaaaacaa actgaaaata ataataaccg aagacatcaa
    32641 aatgaacagg gtggttatag ccaatatatt gaagagcttt ctgcccaatg ccgaattgta
    32701 cgaagccgtc gacggacaag aagcgataga aatgtttaaa aatgtcgatc ccgatctgat
    32761 acttatggat gtacaattgc ctgtaatgga cggactggag gctacccgga gaataagaaa
    32821 cagcgataat ccccgggcca aagaacttcc catcgttgcc cttactgccg gtgtttccaa
    32881 tcaggaaaag gaaaattgct ttcaggcggg catgaacgat tttttaccta aacccatcga
    32941 aaaagaagct ctcttccaag taataatcaa gaatgtaata ggcccccaaa aagcaactta
    33001 ccaaacagat gacttgcttg aaccggacgt tcctcatttc gatgaagata agttgctgga
    33061 aaagatcgac aacgacaccg acctgatgaa aagtttgttg gaaatggctg ccgaagaata
    33121 ccccaaattc ttgcagcaaa tgaaaacggc ctgtgaaaac aaagatgcct gtagtgttaa
    33181 atccatagcc catacgctca aaggcagtgc ttacaatttg gaaatgacag ccatgggaga
    33241 tatcgcccga aaaatggaat tccaccacga agaccctgag gttttgctcc tgttgctcga
    33301 aagtcttgaa aaagaatgga agaacgtgca aaacatcttg gagcagaaac aataattatt
    33361 taacataaac aaaatacaat aaagccattt taatatgtta tatttgcgac ctatgggata
    33421 gtttgtactt ttcctgccta aaagtagatc actatgtcgc acgataaaaa acaccttata
    33481 tacgtcatcg atgacaatcc gatgaactta ttgctaactt ccaagattct tgaaagttac
    33541 ggctttgaaa cccagactgc cgaaagcggt gccgcggcac tgcaactgat cagacaacaa
    33601 agaccttcac tcattctgct ggatatcaat atgcccaata tggacggtta cgaggtgtgt
    33661 cgccggatta aagccaaaga ggactgggcg gatattcccg ttattttcct gacagccaat
    33721 actcaaaccg aaaatttggt cgaaggattc aacgtcggag gagtagatta cataacaaaa
    33781 ccttttcgca gagaggagct atgggtgcgg gtaagtactc atctcgaact ggcggaatcc
    33841 agacggacca ttatagaaat gaaccgttcg agagaccgcc tgtattccat tatcgctcac
    33901 gacatacgct cacccttgtc ggccatactg caaaccctcg aggctataga tcaaggctat
    33961 atcgggctgg aaggtgaaga actcagagaa ataataaagc tgctcggaga gcgcactaaa
    34021 gaaacaagta ccttgttgaa cagcttgctg caatggactc gcgtacaaac cgacagtcag
    34081 cggcttgagt ttaaatcgac cgatctcttt gttcttattc aaagttgcgt tgatttgttg
    34141 cgtcccaacg ccgaacaaaa agaattgagc atagaagtag aagtggaaga ggaactcaaa
    34201 gcctggtgcg atgagctttc catacacaca gtggtgcgaa acctgctttc caacgctatt
    34261 aaatttaccc ctcgtggagg aaaggtaaga attggagcag gcaaaaaaga cggacaggct
    34321 atgatttatg ttgcagacaa cggcgtagga atggactccg aaaccgtgga gcgcattttt
    34381 gcaaaagacc agcactttac aagtagcggt accgaaaacg agcagggtac cggtttgggt
    34441 ctgatgctgg tcaaggattt tgtaaaaaag aacaacggta gtctccaagt ggacagtgag
    34501 cccggggagg gcactgaatt cagggtgttc cttccattga ataattagct gaattatgga
    34561 aacatcaggc agaaatattt taattatcga cgatgatcct gtttacagaa gattatcgtc
    34621 ttccatcctt aaggaacgtt ttacggtgtt taccgcagat gccccttcgg caggattcga
    34681 aatactcaaa aacgaaaaga tcgattatgt gatttgtgac taccggcttc cggaaatgga
    34741 cggtttgggt gttttagaaa gaatcaaaac cgatcatcct catatcgagg taatcatggt
    34801 aagcgattcc ggcaatatgg atacggttat cgaagctttg cgccgtgggg cggtcgatta
    34861 tttcaaaaaa ccttttacac cggcggatat ctggatgtct atcgaaaaaa cgcagcgttt
    34921 taccaaactg aaaaaagatt ttcagttgga aaaaaacaaa agcagccgcc tcaggcaatg
    34981 ggtcgacaaa gaggtgagca tacagatgat aggggaatct cctgccattg aagaaatcaa
    35041 gaaccaagtg cgcctggtag ctcgcacgcc cgacacttcg gtgttgataa tcggagaaag
    35101 cggcacaggt aaagagttgg tggccagagg cattcacgac ttgagtgctc gaagggacga
    35161 attgttcgga gcagtcaata tgtcggccat tcccgaaagc ctgttcgaaa gtgaattttt
    35221 tggacataaa aagggaagtt ttaccggcgc cattgccgac aaagccggct ggttcgaaac
    35281 caccgacaaa ggcagcctgt ttcttgatga aatcggcgat atgccgcata acctgcaagt
    35341 gaagcttttg cgggtgctcg aagagaagaa atacatcaag gtgggaaccc aaaaggaaca
    35401 gacttttgat atccgcatta tcgcagcaac caacaaaagt ctcgaacaaa tatccaatcc
    35461 caaattcttc agaaccgatc tttttcaccg tatcggcacg tttatcattc atctcccgcc
    35521 cctgcgcaaa cgcaaagaag acataccctt gttggtggaa tactttgtgg aacagttttc
    35581 ggaaaaaatg gccaaatcca acatacaggt agcgcccgaa gtttttgacc tgcttcaaag
    35641 ctatagtttt cccggaaaca tacgcgaact gcgaaatctg atagaacggg cagtaatctt
    35701 gtgcaaagac aacattatca aagccgaaaa tttcggtatt ataaaaaagt tggcccacag
    35761 tgctcctcag gaggaggaca gctatgattt acaggaagtc gaaaaaagag ccatcagtaa
    35821 agcattggat atcagcggtg acgatcagga agaagccgcc aaattgctga atattgatag
    35881 caagaccctc tccaaaaaga tgaagcaaca cggtcttttg taaaaccgat tcagaagatg
    35941 aaaaaaaaca gcccgaccct tttagcgatt ttaattatca taacaacagt cgtgttgttg
    36001 ttgatgtacc gcagccccaa caatatttac cgtcccgacg aaaaatacag agccatatta
    36061 cgtgcggtca aagctccggt ccttcccgaa aaaaccctgt cgatactcga cttcggagct
    36121 ttgggcgatt ctgttaccga ctgcaggccc gccttcgaaa aagccatgca agcttgccgg
    36181 gaattgggcg gagcccggat agtggtaccg cggggtaagt attttttgga tggacccata
    36241 catctggtta gcaatgtttg tctggatttg agggaaggag ccgaactctt tttcggttcc
    36301 aatccccgca gttacctgcc ggtggtacgc agcagttggg aaggtacttt tctgtacaat
    36361 tacagccctt ttatttatgg ctaccaacta aaaaacattt ccatcatagg taaaggcagc
    36421 atagacggaa gggcagccga aggttttgcc gaatgggccg atctggacga cgatgatcag
    36481 gctctgagcc gcgagatgaa ttacgccggc acgcccgtcg aagagcggat ttttggcgaa
    36541 gggcattttt tgcgtcccca gttgattcaa ctttttgaat gtaaaaacgt gctgatcgaa
    36601 gacgttatgc tcaaagattc ccccttttgg tgtttgcatt tgcttaaatc cgagaacatc
    36661 accgttaggg gaattcgctt ttcggctttc aacaaaaaca acgacggcat cgatccggaa
    36721 tattcccgca atgtgctgat agaaaacgtg cagtttaata attccgacga caacgtggcc
    36781 ataaaggccg gccgcgatta cgaagggcgc aaaaccaata caccctccga aaacattatt
    36841 atccgccatt gccgtttcaa aggcttgcac gcagttgtga taggcagcga gatgtcttcg
    36901 ggtgtaagaa acgttttcgt acacgattgc gatcacggag gttacctgaa aaggggcatt
    36961 tacctcaaat ccaatcccga ccgcgggggt tatatcagga atgtgcacat cgacaacctg
    37021 aaactgggta cagtagaaga ttgttttttt attacttcta attaccataa tcagggcaag
    37081 ggctttgaaa ccgacatacg cgacatacac gtcaataatc tccactgcaa ggaggccgaa
    37141 aatgccggta tagtgattca aggcttcgaa actaaaaaag tccgtgatat ttatttgagc
    37201 aacgtcaata tcgagaaggc tgccataggc ctgtttatgg aacatgccga gaacatcgtg
    37261 ttggacaatg ttatttccgg aggcagggtg ggtgctcctt ctacggccaa gaaaacggat
    37321 ttgcaacgaa ttgagcgaga ataatgcttt tttgagcaaa taagctatct ttgctgacca
    37381 atctaagctg atcatcatga ttatcgagag tagagcctat gcgcgtgccg gtctgttggg
    37441 aaatccttcg gacggttttt tcggtaaaac catatccatc attatacgta atttcggagc
    37501 cggcgtgttg ttgtatgaat cgccggagct ggtgatcgaa gaagacgaag aggaccgttt
    37561 tcgcaacttg cggcatctga cggaaaccat caaaaaaaca ggctattacg gtggtaaacg
    37621 tttagtcaag gccgctgtga agaaattcag cgaatactgc gatcaggaag gcatccggct
    37681 gcccgcccgc aattttacta tacgtttcag caccactata ccccgtcagg taggtttgtc
    37741 gggttccagt gccatcatca cggctacttt caaggccctg atgaaatttt acgaagtgga
    37801 gattcctcag gaaatgctgc ctagctgggt acttaaggca gagtctgaag aattgtctat
    37861 tacggcaggt ttacaggaca gagttataca ggtttacgaa ggctgtgtat acatggattt
    37921 tgatcgtgaa tttatgctca agcatggtcg tggccgttac gaagtgatcg atccggcttt
    37981 gcttcccaag ttttatgtgg cctacaaaac ccgtttgagc aaggtatcgg gcgccgtaca
    38041 caaccccatc agagaaaagt tcgaagccgg cgatcctttg gtttgcgata ccatggccca
    38101 aattgccgaa gtggccgagc aaggccgcac tgctattcta aacaaggatt acgataccct
    38161 cgacaaactg atcaatttga attttgattt acgccgcaaa atctacaaga tcagcccagc
    38221 tcatttagaa ttggtagaag cagcccgctc ctgcggagtt tcagctaaat ttgccggatc
    38281 gggcggaacc atcataggta tgtatcgcga cgaagaatcc ctgcatcgcc tctttattaa
    38341 aatgaagagc ctgaatgcca gggtaatcaa accctatatt atgtaacaaa agcataacac
    38401 gaaaacgatg attcgaaaag cagtcatccc ggcagcaggg ctgggaacac gatttttacc
    38461 tgccaccaag gcgcagccca aagaaatgtt gcctattatt gatattccca ccatacaata
    38521 cgtggtacag gaatgcgtag aatcgggcat agaagacatt ctgataatca cgggcaaagg
    38581 caaacgttcc atcgaagacc atttcgaccg caatctcgag ctcgaagagc gcttgctcaa
    38641 agaacgcgaa gacgaatatt ggtacaacga gctcaggcat atcgacagca tggccaacat
    38701 ccattttgta cgtcagaaag aacaaaacgg cttgggcgat gccatttatt atgcccgctt
    38761 tcattgcggc agcgaggctt ttgccgtttt gttgggcgac agcgtcaacg attcggttat
    38821 tcccatcacc cagcaaatga tcgacaccta cgagcaatat cgtcatacgg taattgccgt
    38881 gcagcaggtg gataaagaca aggtggaccg ttacggcatc gtgggcggaa cttttttgag
    38941 cgaccgtatt atgcaggtct ccgaaatgat cgaaaaaccc gctcccgaca aggcgccctc
    39001 gaactgggcc gtggccggac gttatattct gactcccgaa atttttcagg ccatagaaca
    39061 aacgccgcgc ggtaagggca acgaaattca gcttaccgat gccctgcgta tcttgctgca
    39121 aacagagagc attattgcca tgaaaataga aggacgtcgt tacgacatag gcaataagct
    39181 cgactacctg aaaaccaccg tagattttgc tcttaaacgc aaggaattcg ccaacgattt
    39241 tgcggcctat ctcaggaaaa aactcaatga attcgataag gtttgagctt cggcaaaaac
    39301 caaaaacgga ctaatctcac taacttaaac acgttttatt gaattatgaa aaccttcatc
    39361 agccttttaa ttacatctct attatcgctc aatcttttac atgcccaata catttccaag
    39421 gtatggaaag gcgatttggg cgacgggcgc tatcaaaatc ccatcataaa tgccgattat
    39481 tccgatcccg atgtctgcct gggtgccgac ggatactata tggtggcatc cagttttaat
    39541 tgtgttcccg gtttacccgt gttgttttcc agagacttag tcaactggag tttggtaggc
    39601 catgccttga gcaggcaggt acccgaagat ttttacaaca aggttcaaca cggcagcgga
    39661 gtttgggctc cttccatacg ctatcacaac aaggaatatt acgtatactg gggcaatccc
    39721 gatttcggta tttacatggt taaggcttcc aatccccgcg gtccctggac ggagccgcat
    39781 ttggtcaaag ccggcaaagg cctcatcgac gtttgtcccc tttgggacga agacggcaaa
    39841 gcctatatgt cttttgcgct ggccggtagc cgggccgggc ttaagtcggt gttgctcgta
    39901 gctcccatga gccccgacgg caaaagtctg atcggtgaac cgcgcataat ctacgacgga
    39961 cacgaaaaaa atcccaccat agaagggacc aagtttcaca aacacgaggg taagtactat
    40021 atctttgccc cagccggcgg agtctctacc ggttggcagg tggtgctcag ggccgatcag
    40081 ccctatggtc cctggcaggt gcgcacggtg atgcaccagg gaaaaaccga gatcaacggc
    40141 ccgcatcaag gcgcctgggt cagaaccccg cagaacgaag actggtttat ccattttcag
    40201 gacgttggcg ttatgggaag ggtaactcat ctcaatccca tgcaatggaa aaacggctgg
    40261 ccggtcatcg gcctcgacgg tgacaacgac ggcataggtg aaccggtaag catctatcgc
    40321 aaaccggtcg tgcccgtacg gctgcctgtt gcaacgccgg cagagagcga cgaattcgac
    40381 aaagccaccc tgggattgca gtggcagtgg caggcaaacc ccgatttcag atggtattat
    40441 ctggaccccg ccagtggtgg caatttgcgc ttgtattctt tttacacccc cggggccatc
    40501 agtttgtggg atgtgcccaa tttattgctt caaaaatttc ccgcccctaa ttttaaagtt
    40561 accgccaaag ttcgttttga gcccgacaaa cgctataagg gcgaacgtgg aggcctggtg
    40621 gtgatgggca tggactacgc agtgctttgt atagagaaca ccgaggatac cctgttactt
    40681 acccaaaaca cttgcaacaa agccgacaac aacggcacgg agagaatagt ggctgccgtg
    40741 cctttggctc aacaagatat ctacctcagg gtggaggtaa aagaaggtca acattgtgct
    40801 ttccattaca gcctcgacgg ccagaaatat atacgtgtcg gcaccgaatt caaagcccgc
    40861 gaaggcaaat ggataggggc caaagtcggt ttgttttgta cccgcccgca ggaatcgaac
    40921 gacggcggat ggttggatgt ttcgtatttc aggattgaac cctaatgaaa aaacacttac
    40981 tgtgttgcct gctggccttg cttggcttcg cccttcctgc agcaggcata accattagcg
    41041 gatatatttc cgacgaggag acaggcgaaa cacttataaa cgccaccgtt tacgacagta
    41101 tctcgggcaa aggagctgta agtaatctct atggtttcta tacgcttacg ttgcccgaag
    41161 ggccggtgca tcttatctat tcttatctgg gttataaacc tcagcgcttt gccttctggg
    41221 ccgatagcga ccgtcaactg acggtacgtc tgcaatccgg tgccgaattg gaagaagtcg
    41281 tggtaagcgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tacaaaggca
    41401 atttcccggc tcgtttcgga ggcaggttat cgtcggtggt ggatatccgt atgaacgacg
    41461 gcaatgacaa gagctaccac ggcaatgtaa gcgtcgggtt gatctcttcc aaattcaatt
    41521 tcgaaggccc cattgtacgt gagaaaacca gtttcaatct ttcttttcga cgtacctacg
    41581 ccgacctgct gcttcaaccc atacttcagg ccacgagacg cggtatggat gaattggata
    41641 aactgtcggc gggttattac ttttacgatt tcaacgccaa gatcaatcat aagttttccg
    41701 acagagaccg cttgtatctg agtttttatt cgggcgacga tgccatttat gccaaggtaa
    41761 gaaataagta taccgaatcc aagacctata aatcgacttc cgccatcggg ctggactggg
    41821 cctggggtaa tctgattacg gctctgcgct ggaatcacat catcaacagt aaaatgtttt
    41881 tgaataccac agcctcctat acccgttacc gtttcgatat gtccatcggg ttgaaagatg
    41941 aaaattggga ataccagcct ccaagctatt tagccacgga cattatgatg ggttatcgtt
    42001 cgggcattga ggattacacc atcaaagccg atctggatta tacccccaat cccgggcata
    42061 ccgtcaagct gggagccaac tatacttacc acacttttaa gcccggtgtt acggttgcca
    42121 agttccggat agacgacgac agcgttcatc aaaatctttt agataccgtt tttggcgata
    42181 gctttgtgta cgctcacgaa accatgcttt acgcggaaga caacatcgat ttgggccgct
    42241 atgtcaaggc taatttaggt ttgcattatt ccaccttttt tgtgcagcag caattttatc
    42301 attcgctgca gccgcggctg ggtttgcgtt ttctgtttag cgacaatttc tcgttcaagg
    42361 ccggttacgc catgatgaac caatacattc atctcctgtc caacagcgac atcagcctgc
    42421 cgaccgatct ttgggtgccg gttaccaagc gcattacacc catgaactcg catcaggtgt
    42481 cggccggttt gttttataac ctcaagaatc tagttgattt atcggtggaa ggttactata
    42541 aatcgatgca caatatcctg gaatataaag acggtgcctc ttttatgggc tcaaccaccg
    42601 gttgggaaga caaagtcagc atgggtaggg gttggtccta cggactggaa tttttggcgc
    42661 aaaagtctta tgggcgttac acgggttgga taggttatac ctgggccaaa tcggaacgct
    42721 tgtttgaccg ccccggtcag gagctcaaca acggaaaagt gtttccggcc aagtacgatc
    42781 gtcgtcacga cctgagcatt acggcttcct ataaactcaa cgaccgcata gattttgcgg
    42841 ccagttgggt ttacagcagc ggcaattgcg ccaccttggc tttgcaaaat tacgaaggga
    42901 gcccggtgcc cgacagttat cagtattatt tctccagtct tccatacatc gagtcgcgca
    42961 acaattatcg cttcgaatca tatcagcgac tggatatcgg ggtgaatttc cgtaaaaaac
    43021 ttaagcgcgg cacccgtacc tggaacatta gcgcttacaa tgcttacaat cgcaacaatc
    43081 ccttttttgt atatccgtct cattcggaac gatacgatcc tgttagccgg caatacgtga
    43141 caaaaagggc gctcaagcag gtttccattt tccctctcat tccttctatc agttattctt
    43201 actctttttg attcggccct atgaaaaaag cgatattata tcttactgtc tttatgctct
    43261 ttgcggcttg cgaaaaagag gttcggttta agggtgaggt caccgagccg ctgctggtat
    43321 taaatggttt tgctcaaccc gattcactgt tggtgattag gctgacggcc agcaggttct
    43381 ttctggaaga agtcgattcc ttcgttacgg ttaaggatgc ccatcttcgt ttatatgtgg
    43441 acggaacgta caaagaggat ttgcaatcgc agaaaaacgg ctactatatc agttcctacc
    43501 gccccaaaca gggcgaagtg ttgagtatcg aagccgaagc tcccggtttt gaatctgtcc
    43561 gggccgaagc cctgttgcct gtggttgcag aactgctcaa agtggatacc tccataacct
    43621 tggaggggga actatacgat ctttcgtacg attgggatcc ggaagtcgga gattatatta
    43681 ttgatacgat aggtttgtat accaattatc aggtaagcat gactttgagc tttcgggatc
    43741 cgcctacccc cggcgattat taccgggtgg tggtaaagca tttctatggc aaagaatact
    43801 atgatggtta ttacgatttt accgttgaaa atttttacac ggccgaaatg ggagacttgt
    43861 tgggtccggc cgaaacccaa aacgagtacc agatatacgc cgatgagctt tacaacggta
    43921 aaacggtcga tttgaaattg agttttgctg cacataccta ccgtgattat cagggggaac
    43981 ttcccaattc gaccaccagt attcaggttc aattgcagaa tatatctcca tcttattatc
    44041 tgtatctcaa gactttttca gaatacaata attccattga ctttttttcc gaacccatac
    44101 aagttttcag taatgtaagc ggaggaattg gaatttttgg tgcttacagt tccaatgtta
    44161 ttgaactgaa gtttaattga tggagatcaa agaatcctac aaagcaagat tacaagaaat
    44221 aaaatactaa acgattcaag ctatgaaaaa acccgcattt aaaaatattg cattgttgtg
    44281 tggttcaatc ctggctctgc tgggcatgtc ctgcactcaa aaagaagaca tccggctttt
    44341 tgtggccggc gattccaccg ctcagagcta tcgtgtggaa caaaccctga tgcggggctg
    44401 ggcgcaatac ctcgagctct tcttcgacga acgggttcaa gtcgaaaatc gctccatggc
    44461 cggtcgcagc agcaagtcct ttcgcgaaga gggccgttgg aacaaaattc tcgaagatct
    44521 ccgcaaaggc gatgtggtgt ttattcagtt tggacacaac gacacttcga ccaagcccga
    44581 acgtcatgca tcgccggcgg attaccggca aaatctgcgc cgttttgtgc aggaagtacg
    44641 cgagcgggaa gctacgccgg tgctgctcac ttcgatcgtg ttgtgtacgt tcaacgattc
    44701 ggggcagctc gtagaccgcc gtctgaagga atatcctcag atagtgcgcg atctggcagc
    44761 cgaggaaaaa gtgatcctga tcgatatgtt tcgactcagc tttgacctat tgcaggaact
    44821 cggtccggag gaagcccgca agctttacat gtttctcgaa ccgggcgaag atccttccag
    44881 gcctcaggga tctaacgacg acacccattt aagagaaaga ggcgccaaac aattggcggc
    44941 cctggtagcc gaagaaatcc gccaggcccg gatcaagcct ttgtaccgct atttgctcga
    45001 caaagaagaa tggccgcagt aatctcgctt tatttgttgc gtccaaaaat tttttcttgt
    45061 actttcgcat cgttttttag aacgcggaaa tagctcagtt ggtagagcat cagcttccca
    45121 agctgagggt cgcgggttcg agtcccgttt tccgctccac cggtcatcga atatttcgat
    45181 tcttatctga agtaattctt atctgaagta attcttgtct gaagtaatat tagtttgaag
    45241 tgatattgct ctgcagcaat gccgggatcg gt
//
LOCUS       DAOSTD010000003        43297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_979_length_43297_cov_14.394362, whole genome shotgun sequence.
ACCESSION   DAOSTD010000003 DAOSTD010000000
VERSION     DAOSTD010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 43297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43297
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_979_length_43297_cov_14.394362"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLI37_00340"
     CDS             complement(<1..269)
                     /locus_tag="PLI37_00340"
                     /inference="COORDINATES: protein motif:HMM:NF016175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOQ57497.1"
                     /translation="MPSTIILANGEFPRKPGLLHLLRQAEHVVCCDGAAEKLLNFGRE
                     PSVIVGDMDSLSPSLKELYADRIITIDEQETNDLTKAVNYCERAG"
     gene            complement(272..2488)
                     /locus_tag="PLI37_00345"
     CDS             complement(272..2488)
                     /locus_tag="PLI37_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ57498.1"
                     /translation="MKRVFLCLAAGIFVVHSMAAAKTDSIRQLDLNDVVVIASYAGEN
                     TPVTHQNLSKAEIRTLNVVSTLPQVLWMTPSLVHTAENGTSTGNTAMRIRGTDASRIN
                     FSLNGIPMNNPESQEVYWVNIPNLTGSLQSIQIQRGVGVSANGTGAFGASVNLLSERP
                     AAENYIESSTTAGSYGTFEQSVSMATSRLPGGVQMQLRYTALNADGYLRNGWSRHQSL
                     YGTLGKSWEKAQLSMNYIYGNQHTGITWEGASKEQIALNPAYNPAGAIKDGLYYGNES
                     DNYRQHHIQLFYSRELTETLLLNTGLNYTDGYGYYEQYKQNRKFSSLGLSNQLIDGIE
                     YDRSDHIRGKFMANGFYTGLFNLNYQPGAFRLQGGGMYSFYQGVHYARLLWLEHNSSI
                     PANYEWARNFGEKTDGNLFVKAEYALRNKLFLYADLQYRNVRYELEGIDDDDLLDLSQ
                     SHQWQFWNPKMGVNWRIDQSRKLYLSAGIANREPTRADLKDAVKGGSRRVLPERLYDL
                     ELGYSVNKPKWMLALNLYDMEYHNQLIPTGKLNEVGYKLMSNVPYSYRRGMELSAGIR
                     PFSVLQLDANLSLSTNKVKDFSLYYSTYDNASDWNEILDQSRQISSYYSLADLPFSPA
                     CIATARLQYQPVPSLQLSLMGKYIAEQQFSYIQHEEFRLPAYTQFNFSAVYSGCLVGN
                     SDFELGFYINNLLGNQPLCNAWGYEVAFLSGEEPYRETGYYILAPRHYMLKFVFKI"
     gene            2773..3966
                     /locus_tag="PLI37_00350"
     CDS             2773..3966
                     /locus_tag="PLI37_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOQ57499.1"
                     /translation="MGKVLIIGAGGVGSVVAYKVAQHPEVFSEIVLASRTKAKCDKIA
                     AFVGNKRIQTDQVDADSVEQLVALFKKHRPELVIHVALPYQDLTIMDACLACGVHYLD
                     TANYEPKDKAHFEYSWQWAYKERFEKAGLTAILGCGFDPGVTGVWTAYAAKHHFDEIH
                     YLDIVDCNAGDHGKAFATNFNPEINIREVTQKGRYYENGQWIETEPHQIHKTLTYPEI
                     GPRESYLIYHEELESLVKNFPTIKRARFWMTFGEEYLTHLRVIQNIGMAGIEPVMFKG
                     MEIIPIEFLKAVLPDPGELGENYTGQTSIGCRIRGIKNGKERTYYVYQNCSHQAAYKE
                     TGAQAVSYTTGVPAMCGAMMFMKGLWRKPGVFNVEEFDPDPFLKALAENGLPWHELFD
                     VDLEL"
     gene            complement(3996..4493)
                     /locus_tag="PLI37_00355"
     CDS             complement(3996..4493)
                     /locus_tag="PLI37_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOQ57500.1"
                     /translation="MKKIAILSDTHDYWDERYIPYLEICDEIWHAGDIGSVLLTQRLE
                     AIRPLRAVYGNCDGYPLRYNFSSHLFFELEQVRVLMTHIGGYPGRYDSSALALIKHHK
                     PDLFVCGHSHIAKVMFDRRHDMLCVNPGAAGKQGFHTIRTLMRLELNEGNIENLEVIE
                     LGKIV"
     gene            complement(4496..6076)
                     /locus_tag="PLI37_00360"
     CDS             complement(4496..6076)
                     /locus_tag="PLI37_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HOQ57501.1"
                     /translation="MNLDPAFVRQMRRLLPAESAAFMRSLEEDIPVSLRLNTAKCSGE
                     KTLNCLPAKVQSAVPWCPDAYYLEQRPSFTFDPFFHCGLYYVQEASSMFTARAIHAAL
                     QAMKMSGRAVRMLDLCAAPGGKSTLAASVLPEGSLLLANEIVPSRAVILEENLVKWGK
                     AEVVVTKNEPADFAEFEHAFDLILADMPCSGEGMFRKEPEALAAWSEDNVLGCALRQR
                     NILAQCWNSLRPGGCLIYSTCTYNTEENEKNLLWAAENLGARVLPVECNPDWSIHPAI
                     GADIPAYRFFPHRTRGEGFFLALLQKEESLAANSGTGAAYSRTDAVNSRTGLADSQIL
                     AGNLQTGLKSYPQADFHELFKPKKGKKKLPSRKKSGKFVNEVVDKKKLTYLNSLFDEP
                     EAYEISLLPWGVYRAFPSQHAAFLARISHSLRIVRAGLYLGEYKGNQFIPDVSLALST
                     AVEAGKHFPSYELHRQQALAYLKRESLQLSEDCPQGQVLVTYGRVGLGWVNNLGIRAN
                     NRYPASWKIRSQLPVNQF"
     gene            complement(6082..6867)
                     /gene="mazG"
                     /locus_tag="PLI37_00365"
     CDS             complement(6082..6867)
                     /gene="mazG"
                     /locus_tag="PLI37_00365"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOQ57502.1"
                     /translation="MHDRQSKMEAFGRLLDILDQLRLKCPWDKEQTFESLRPLTLEEC
                     YELSEALLQKDHPDVCKELGDILLHIVFYSKIAEEQQLFDIEDVCDKLAEKLIFRHPH
                     VFGTVSVQDAGEVEKNWEKLKLKEKNGNGSVLAGVPSSLPSLIKAYRMQDKAAGVGFD
                     WEDRRQVWAKVQEEIREFQREIEKDDKDGMEDEFGDLLFSLINVARLYGINPDNALEH
                     TNKKFRERFNYLEEETLKKGLSLKETSLDQMEVIWQQAKKFFP"
     gene            complement(6874..7542)
                     /locus_tag="PLI37_00370"
     CDS             complement(6874..7542)
                     /locus_tag="PLI37_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57503.1"
                     /translation="MIIAHQKYYRGIIALVLFCFFTACTKVGGFTIDVQGPDIEFKMA
                     FFDADNQREALKMASSGNHKSNQDAFLDYRPIAKSDSIDALDIEAFLSEVGQTFNSVV
                     EKAELKNSKMRLSEDTVDFSGVNSFLIKVRQFGEEEFVPFCLTEVNTKTNSKQLSVES
                     VYFKPNEVFKLIECGFEAILYAKISSNNKKPACLRDGIHFEFLSGTVIAVKVKAGALF
                     DLDI"
     gene            7773..10430
                     /locus_tag="PLI37_00375"
     CDS             7773..10430
                     /locus_tag="PLI37_00375"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOQ57504.1"
                     /translation="MELPSKYNPAEVESKWYQYWLQHNMFHSTPDEREPYTIVIPPPN
                     VTGVLHMGHILNNTIQDILIRRARMLGKNACWVPGTDHASIATEAKIVEKLKNEGIKK
                     TDIGREAFIEHAWEWTHKHGGIILEQLKKLGASCDWDRTCFTMDDVRSESVLKVFIDL
                     FEKGLIYRGVRMINWDPKAQTALSDEEVIYKEVQSKLYYLRYKVQGEDRYATIATTRP
                     ETILGDTAVCINPKDESKAWLRGKKVLVPLIDRPVPIIEDEYVDIEFGTGCLKVTPAH
                     DVNDYMLGEKYRLESIDIFNNDGTLNKHGAMYAGMDRFEVRSKIEKDLKAAGLLEKIE
                     DYTNKVGYSERTDEAIEPKLSMQWFLKMSDLAKPALEAVEKDEIKLIPPKYKNTYRYW
                     MENVKDWCISRQLWWGHRIPAYYLPEGGFVVASTPEEALEKANALRMKNVCPCGCSGL
                     KPLKPEDLRQDEDCLDTWFSSWLWPISVFDGINEPDNKDINYYYPTNDLVTAPDILFF
                     WVARMIISGFEYRKEACFKNVYLTGIVRDKLGRKMSKSLGNSPDPIELMDKYGADGVR
                     MGMMLAAPAGNDVLFDEALCEQGRNFNNKIWNAYRLISGWKVDPKLDQPKASALAVEW
                     FEARLGKTLAEMDDLFDKYRLSEAMMCVYKLFWDEFSSWFLEIIKPAYQQPIDSITHK
                     AVFGFFEQLCLLLHPFMPFITEELWQHLEQREPGESLMLQQQRKPTAYNQVILDKFEL
                     VKAVVSGIRTIRLEKNLPVKTALALEIQGKHDASFDSLIVKMGNISSITQVSAKASDA
                     ASFLVNTTEYSLLLGALIDTEEERKKMNNELQYQEGFLQSILKKLNNPNFISRAPEAV
                     IEAERKKMADAKNKIQAIKDSLDSLESNK"
     gene            complement(10432..11769)
                     /gene="gdhA"
                     /locus_tag="PLI37_00380"
     CDS             complement(10432..11769)
                     /gene="gdhA"
                     /locus_tag="PLI37_00380"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOQ57505.1"
                     /translation="MEVNRFMANLEMKHPGEHEYLQAVREVISSIEKVYNEHPEFDKA
                     GILERIVEPDRIFIFKVPWVDDAGNIRVNTGYRIQFNNAIGPYKGGLRFHASVNPSIL
                     KFLGFEQTFKNALTTLPMGGAKGGSDFSPKGKSAAEVQRFCHNFMLELWRNIGPDTDI
                     PAGDIGVSGREIGYMYAMYKKLARVNSGVFTGKALGFGGSLVRPEATGYGCLYFVNQM
                     LETKGMSIEGKTVAISGFGNVAWGAALKATRMGAKVVTLSGPDGYIYDEAGLDDEKIA
                     YMLELRASNNDVIAPYAKKFKAEFIPGKKPWERKVDVALPCATQNELNLQDAKNLLSN
                     GVICVGEVSNMGCTAEASDAFVESRILFAPGKAVNAGGVATSGLEMTQNAMHLSWTAE
                     EVDQKLRQIMSAIHDQCALYGKEADGYINYVKGANVAGFMKVAKSMMDQGYIS"
     gene            complement(11897..13903)
                     /locus_tag="PLI37_00385"
     CDS             complement(11897..13903)
                     /locus_tag="PLI37_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOQ57506.1"
                     /translation="MSKTEENKPVTALPENAYRELKEGELYHPVMSPGRKYPEATVWS
                     VSWGVLMAILFSAAAAYLGLKIGQVFEAAIPIAIIAVGLSSLTKRKNALGENVLIQSI
                     GSSSGVIVAGAIFTLPALYILQAKYPDMSVNFIQVFLSSLLGGVLGILFLIPFRKYFV
                     SEMHGKYPFPEATATTQVLISGEQGANQAKPLVVSGLIGGLYDFVVATFGSWTEVFTS
                     RVSQFGTMMADKFKLVFSINTGAAVLGLGYIVGLKYATVICLGSALIWFVFVPLLPLF
                     GDTFLQFLNPAISDGVAGMSPEVIFSSYARHIGIGGIAMAGIIGIVKSWPVIKSALGL
                     AAREMKGQGAKTAEQVTQARTSSDLPMKIILIGIVLALLAVFVFFFFGVMHLNLLHAI
                     VGILIVGIIAFLFTTVAANAIAIVGTNPVSGMTLMTLILASVIMVAVGLKGSTGIVSA
                     LIMGGVVCTALSMAGGFITDLKIGYWLGSTPAVQQSWKFLGTLVSALTVGGVLIILNK
                     TYGFVGENALVAPQANAMAAVIDPLMSGTAAPWWLYATGALISLILNFFGVPALAFAL
                     GMFIPFQLNIPLVVGGLINWFVSGRSKDSKLNTARNEKGTLIASGFIAGGALMGVVSA
                     ALRFGGMDWTLTSWANTKGAEMLSLLMYALLIGYFVFSVLKKKK"
     gene            complement(13915..14682)
                     /locus_tag="PLI37_00390"
     CDS             complement(13915..14682)
                     /locus_tag="PLI37_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOQ57507.1"
                     /translation="MKREFGLIGYPLTYSFSQVYFNEKFQSEGIEAVYENFPLKRIEE
                     FPSLIQSRPALAGLNVTIPYKQSVIPFLDELGPEAASIKAVNVIVPEIKEGKIRLIGR
                     NSDYIGFRDSILPEIERLKASCPQQLKALILGTGGASKAVYYALQQMGIETSFVSRDA
                     AKAEYTYAMLNKELMDRFKIIVNTTPLGMAPDTDSCPDLPYRYLGRGHLLYDLVYNPA
                     LTRFLSLGQQAGARVMNGAGMLKIQAEAAWKMWNGQL"
     gene            complement(14679..16052)
                     /locus_tag="PLI37_00395"
     CDS             complement(14679..16052)
                     /locus_tag="PLI37_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ57508.1"
                     /translation="MKSREDDSIRALVKRYEQQRSNGLSQYFDVDEFDRIADYYLDRG
                     RTNKFSEVVNSALRIHPNSTVIHLKRAILYMETGEPMKALQLLGRLPEQEETDFVRAA
                     AYLKLKRKKEGLALLKDMVAREQYDKDLACLEAVSVLSAENLYKEALDFVRLGLEHNP
                     ENTDLMSEGAFCAEQLNDMPASEHYYRMLLEVDVYSYENWFNLGQILFSQERFEEACK
                     AYDFALVSNPGFMPAVLQMGHACFQMEHYEDAAKFYLDYLLAAIEESLPSAEGNSIDD
                     LMVFVGEAFEKAEHYESAIEWYEHALTTNPGNLQAYTGMGICYMELKDYKQSLRYLEK
                     AIKMDDKQAEIWVYLAEVLVDMDKNEQAIVAYLHALSLDPSQAEVLFALGNLYYDQQK
                     FELALKYYSDAELLDATFPNIHLYKALAYARNGDMLQADDALKLALLSDVHARELFNK
                     IIGEEQK"
     gene            complement(16076..17773)
                     /locus_tag="PLI37_00400"
     CDS             complement(16076..17773)
                     /locus_tag="PLI37_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOQ57509.1"
                     /translation="MTVVDTNSEGSKKSLNFIEQIVENDLKEGKNRGRIATRFPPEPN
                     GYLHIGHAKAICLDFGIAQKYGGTCNLRFDDTNPTKEDVEYVDSIMEDIRWLGFSWDN
                     LFYASDYFGKLFQFAQELIKKGLAYVDDQSAEVIAQQKGTPTSPGVNSPYRNRSAEEN
                     LDLFLRMQAGEFDEGSRVLRAKIDMAHSNMHFRDPLMYRILKQAHHRTGNTWNIYPLY
                     DFAHGQCDYFEGITHSLCTLEFEIHRPLYDWFIDHLQEGDYRPRQIEFNRLNITYTIM
                     SKRKLLQLVEGGFVKGWDDPRMPTLCGLRRRGYTPESIRNFIDRIGYTKFDGVIDQSL
                     LEFAVREHLNKIAPRVSAVLNPVKLIITNYPEGKVEWNEAINNPEDESAGSRSIPFAR
                     ELYVERDDFMEEAPKKFYRLTPGQEVRLKNAYIVQCTGCKKDADGQVQEIYCTYDPLT
                     KEGGPESKRKVKATLHYVTVEHSLPAEVRLYDRLLTVENPAEDKERDFMDMLNPDSLK
                     VLKDCRVEASLQDARPLDHFQFLRMGYFNLDPDSRPGQLIFNRTVGLRDTWSKIAKK"
     gene            18115..19521
                     /gene="uxaC"
                     /locus_tag="PLI37_00405"
     CDS             18115..19521
                     /gene="uxaC"
                     /locus_tag="PLI37_00405"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOQ57510.1"
                     /translation="MKAFMDENFMLENEVSEQLFHQHAKNLPIIDYHCHLNPEYIAKD
                     HKFDNLGQIWLEGDHYKWRAMRTNGIDERYCTGKDTSDWEKFEKWAETLPYTMRNPLY
                     HWTHLELKTAFGVNKILKPETAREIFDECTAKLRTPEFSARGLMRKYNVETVCTTDDP
                     VDSLEHHIALKKEGFEIKVLPTWRPDKAMAVEDPKSFRAYVEKLAQAAGTKISRYNDL
                     IAALRIRHDFFASVGCKLSDHGIEEFYAEDYTQAQIEAIFNKVYGGKELSQEEILQFK
                     SAMMVEFALMDAEKDWTQQFHYGAIRNNNSRLFAQLGPDTGFDSIGDFNVGKNMSKFL
                     NRLDKNNQLAKTIIYNLNPRDNELIATMLGNFQDGSVAGKIQFGSGWWFLDQKYGMIA
                     QMNSLSVLGLLSRFVGMLTDSRSFLSYPRHEYFRRIMCNLIGVDVVKGEIPYSEMDFL
                     GKMVEGVSYYNAKTFFKF"
     gene            19594..20637
                     /locus_tag="PLI37_00410"
     CDS             19594..20637
                     /locus_tag="PLI37_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOQ57511.1"
                     /translation="MSKRVVTFGEIMLRLATPGYLRFSQTSSLTATFGGGEANVAVSL
                     ANYGIKVDFVSRLPLNDMATSCVMNLRKYGVGTDHLIYGGERLGIYFLETGAVARPSK
                     VIYDRAHSSIAEIQPGMIDWRKIFKGADWFHWTGITPAISQGAADACLEAIQIANEMG
                     VTVSTDLNFRKNLWKYGKSASEVMPKLVAGCDVILGNEEDAEKVFGIKPEGFEVAHTG
                     GEVNAAEFESVCKQLMAKFPRAKKVIITLRGSINANHNTWRGCLYSDGSLKISKAYDI
                     THIVDRVGGGDSFMGGLIYGLLTYKEDDQKALEFAVAASCLKHTIYGDFNMVSVAEVE
                     KLMGGDASGRVSR"
     gene            20649..21320
                     /locus_tag="PLI37_00415"
     CDS             20649..21320
                     /locus_tag="PLI37_00415"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOQ57512.1"
                     /translation="MAKFTKQQVINTIYTSGMVPVFYNKDIEVSKQVLLACYRGGVRV
                     FEFTNRGDFAQEVFKELVKYAEKTCPEMIMGAGSVVDAGTASLYIQLGANFIVGPLFN
                     PEVAKVCNRRLIPYAPGCGSVTEIGYAQEAGCDICKIFPAGNVGGPSFVKNVMAPMPW
                     SKLMVTGGVTPEESNLKAWFDAGVTCVGMGSNLFPADLIKSENWDGITKLCAEAMAII
                     QKVKK"
     gene            21358..22824
                     /locus_tag="PLI37_00420"
     CDS             21358..22824
                     /locus_tag="PLI37_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ57513.1"
                     /translation="MSKAQTVTQKMSQHRWVIVALLFFATTINYLDRQVLSLTWDEFI
                     KPEFHWDESHYGTITSFFSIFYAISMLFAGRFVEWMGTKKGYLWSIGVWSVGACLHAV
                     CGLITEAHVGLHSAAELAAATGDLAVVIATFSMYSFLVARGILALGEAGNFPAAIKAT
                     AEYFPKKDRAYATSIFNAGASIGALFAPLTIPPLAKAFGWEMAFIIIGALGFIWMGFW
                     VFLYDAPRKSKFVNSQELEYIEQDKHEEIAEQKKKAAEEEKVPFLKILSFKQTWAFAL
                     GKFMTDGVWWFFLFWTPSYLYTQFDIKMSEGLGMALIFTLYAIVTVLSILGGKLPTVF
                     INRGGQNPYAARMKAMLIFAFFPLVVLLAQPLGMQFAELGQHAAWIPVLLISIGCSAH
                     QAWSANLFSTIGDMFPHGAIATVTGIGGMAGGVGSMILQKGAGNLFVYAGETNMTFFG
                     FVGKPAGYFVIFCICAVAYLIGWIIMKSLVPRYQKIVI"
     gene            complement(23073..24473)
                     /locus_tag="PLI37_00425"
     CDS             complement(23073..24473)
                     /locus_tag="PLI37_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOQ57514.1"
                     /translation="MGLLAIPLLGSVGSVNSLMAAPAPRIESKTNNRIRGFEYPQHPG
                     KLNLNVLDFGVVGDGITINTEALQHAIDRCFVLGGGEVFVPAGDYLTGAIQLRSGVLL
                     RLHSEAILRGTPHFEDYPVMQVRWEGKWIQGRTALIYAVDASYTGIVGGGKIIGHPAL
                     GGRPTSDNPLRHPALIEPINCKNLLFSDFSTSYQRMWCIHPTYCENIHFKDLTIRSTG
                     GNGDGIDIDSCKHVLIERCDIETGDDCIAIKSGRGMEANMLMRTTEDVYIRNCRLSDS
                     IYACIGIGSETSGGIRGVRIENCRFENTRTHAIYIKSRPGRGAFIEDISAEGLDVSGM
                     QGGFLRFNLMGSGLQDQVPVPGLQGIPTVKNFSFKNVRVRDVKDLVDGTSVHPDKPLE
                     GLVLENISGEALWGIRLANVRKAVLRNINVNIREGDALSIYNVQGKGLGKAIEMGFPH
                     KIEAAIAEPDPPYVLK"
     gene            24731..27562
                     /locus_tag="PLI37_00430"
     CDS             24731..27562
                     /locus_tag="PLI37_00430"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOQ57515.1"
                     /translation="MPRLNKHLLTVVLFLPFLLMAQEQLTNLPTLYITTDSTYANGTK
                     VPIQKEVYFSGSLRVVAGSETPGQYDSTIQIRGRGNSTWNLAKKPYRIKLDKKTRLLG
                     MTAKENDWVLLANHADKTLVRNALAFEISKACNIYYTPDYRFVDVILNGNYIGNYMLT
                     DQVERDKDRVNTEKMDTSRLDPNLTGGYVLEIDGFGITGSYDPSKGELFPEAFSSNIG
                     NKVTIKYPDNDEITAAQRQYIIQHYNAFEQSILNYNNTQVAYENICRYLDTTAFVNWY
                     IANELTANPDGVWSIFMKKHRDDDRLYFGPLWDNDISFGNCNRIYSLANDGRSESIML
                     KCFSNNRVKELINKVLTISPIRKMIAERWKVIYQNEMLKDDLIHLLYNMEEELDASQK
                     LNYQKWSTALSSRIYNEIYYGSNISFAMQMEYLRNYLSERIDFLDSFFSAMANEGGID
                     PEDPPLPPVDTATYFSPEPYVWYHLQCQLNDKYLVCQEVAGGYQLDYTYDPETAGDWA
                     KFEISCSKDDENRSVYYLRNKAAKTYVYINAAKAAIPLDPVQKTFFTLYPSPNEAGHY
                     GLMVLSDNSTLHELGIDANGSVVSWPTLNKKGQREYRFIPDRSHFQSVFDPPDPQQAM
                     WDMEENWDNNIPNAYATAIIAEGAKLELSQDETVTVDSLIILPGAEVLNYGTIRADVS
                     LFCSNDSLSASYDYDLEAQLISERVLYRSFNRSQSWYALSLPLDVKSVRNKEMTILQP
                     GKDYYLMEYKADSNRFDSLSLASSFKPKAGPYLFSVSPEREQQAFIFVFDTKSSYQTG
                     NSDYNFTGNTGFKTINIPAAGTYLLEDHLHFVLQEDQQQLSLPAFHAYFSVSGDRLPD
                     SLAIYNMNPANDTLALENISKQGPLLVSRAGLLIIFNMEDGEDFSLFNLDGKLLFKAK
                     ALNNPTYVPLQQGIYLLRYKKRFHKIHIP"
     gene            27584..30673
                     /locus_tag="PLI37_00435"
     CDS             27584..30673
                     /locus_tag="PLI37_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ57516.1"
                     /translation="MKKRIISTFILTAIVLSVSAQKNEWQDPGINAVNRAEMHTNYFA
                     YESREAALKNCRESSSNYLSLNGMWKFYWVKDANNRPTDFFRNDFDDRNWANMPVPGI
                     WEMNGYGDPVYVNTGWAWRNQFRSNPPDIPVENNHVGSYRREITIPDHWKGKEIFAHF
                     GSVTSNIYLWVNGKYVGYSEDSKLEAEFNLSKYLRPGKNLIAFQVFRWCDGTYLEDQD
                     FFRLSGVGRDCYLYARPSNYIQDIRITPDLDAQYKDGSLKIDVSMKGKGELELNLLDE
                     NNETVASTQRNASGKFSLMMNLSNPQKWSAECPNLYTLVATLKGGGQVLEAIPVKVGF
                     RKIELKGDQLLVNGQPVLFKGVNRHEMDPDYGYYVSPERMIQDLRLMKEHNVNAVRTC
                     HYPDNNLWYDLCDEYGLYVVAEANIESHGLGYGERTTAKRPDYALAHMERNQRNVQRS
                     FNHPSVIFWSLGNEAGFGPNFEMCYKWIKAEDPSRPVQYEQARTNEFTDIFCPMYYGY
                     NNCIRYCEGDIQKPLIQCEYVHAMGNSEGGIKEYWDLVRKYPKYQGGFIWDFVDQSLR
                     KFDKNGVMFYAYGGDFNLYDASDGNFCDNGLISPDRLPNPHFHEVGHVYQSIWASASD
                     LEKGEIEIYNEYFFRDLSAFYLEWALLVNGKVCQSGMVDKIDIEAQQTKKLKLDYDLS
                     GISPDKEVLLNISFKLRKAEQLLPAGFVLAKNQLCIRDRSKQSLNFDNILTANIELQT
                     PKVIENDWRFLIIEADNYRIEFNKHNGYLSRWQVKGTDLLNEGGSLTPNFWRAPTDND
                     FGANLQNKLAVWKNPGLRLESFEQAIEDGLVVVRAQYDMRAVSSKLHLTYRINNQGSI
                     EVTQKMTAGADAKAPELFRFGMQLQMPLLMDRIEYYGRGPIENYSDRNNSTDLGIYKQ
                     TVEEQFYPYIRPQENGNKTDIRWWKQTNAGGRGIKIRSVAPFSASALNYSIESLDDGP
                     SKGQRHSQQIPKVDYTNLCIDKVQMGLGSVNSWGAMPRDEYRLPHQDYEFQFVMEVR"
     gene            complement(30772..31791)
                     /gene="holA"
                     /locus_tag="PLI37_00440"
     CDS             complement(30772..31791)
                     /gene="holA"
                     /locus_tag="PLI37_00440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOQ57517.1"
                     /translation="MAKKTENAQEVIREIEAGKRYPIYFLMGEEPYYIDEISNRLLKL
                     VLTESQQDFDQRILYGKDLSVAQLINEARRYPMLAPYQVILVREAQMIRNFEEEFLLY
                     VQQPMPSTILIINYKYKSLDKRRKLYSALEQSALLFESKKMYDNQIPAWITRFLSEQE
                     IQIEAKAAQMLADFLGTDLGRIVGELEKLKLLLKIKPYKITAAMVELNIGVSKEYNNF
                     ELIDAIKQKDQFKAQQIAQYFARNPKNNAMVVTLIVLFDYFSKLLMYHYLPDKSSSNV
                     ASQLGIPPFLVKNYQQGAQHYNANKVVRNISLIRHYDARLKGFEQAALPESELLRELL
                     CLLMA"
     gene            31855..32304
                     /locus_tag="PLI37_00445"
     CDS             31855..32304
                     /locus_tag="PLI37_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOQ57518.1"
                     /translation="MFALNLPGFKYKLRKKDAKNYIFDPLRRKYVFLSPEEWVRQHFC
                     NYLIVHKNYPSGLIANECSIQLEGLTKRCDTVIYDAHMQVFMLVEYKAPTVTLNQEVF
                     DQIVRYNWALQAKYLTVSNGMNHYCCRIDYLDNTVNFLEDIPAYNEG"
     gene            complement(32328..33119)
                     /locus_tag="PLI37_00450"
     CDS             complement(32328..33119)
                     /locus_tag="PLI37_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOQ57519.1"
                     /translation="MSVDSTKAEPKEISVEEKLKALYELQVVASEIDKIKILRGELPL
                     EVQDLEDIIAGLETRIENFKAEIKTCTDRILGCKIDIENSTALIAKYKAQQDNVRNNR
                     EYDFLSKEIEFQGLEIEHAEKKIREAQFSEKAKNEELNLAQEELNERREDLEVKKSEL
                     DEIISETRSEEETLREKAKTIESYIEPRLLQAFKRIRKNARNGLAVVYVQRDACGGCY
                     NKIPPQRQMDIRMRKKVIVCEYCGRIMIDPELAGVETPAEPAGKK"
     gene            complement(33149..33931)
                     /locus_tag="PLI37_00455"
     CDS             complement(33149..33931)
                     /locus_tag="PLI37_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOQ57520.1"
                     /translation="MKVRDICTLIENFAPLKLQEDYDNAGLILGDPATQVKAILVCLD
                     VSEAVLDEALEKGCNMVVSHHPLIFRGIKSITGKNPQERTLIKAIQNKIAIYAGHTNV
                     DAVMDGVNGKICEKLNLSSTAILVPKENMSNVGAGMTGFLTRALEEKDFLILLKQVFG
                     SKMLRHSALTGREINKVAVCGGSGSELISAAIAEGADAFITADVKYHRFAEVDDKLLL
                     IDAGHYETEQFTKDLFVELISKNIANFAVHSSQRETNPVFYT"
     gene            complement(33928..35691)
                     /gene="aspS"
                     /locus_tag="PLI37_00460"
     CDS             complement(33928..35691)
                     /gene="aspS"
                     /locus_tag="PLI37_00460"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOQ57521.1"
                     /translation="MFRTHTCGELRIHDVSKKVCLAGWVQRIRKMGAVTFVDLRDRYG
                     LTQLVIDQEAGAACIETVNKMGREWVIQVHGLVRERSGKNPQLVTGDIEIQVEEIKVL
                     NAAATPPFTIEEQSDGGDELRMTYRYLDLRRPPLSRNLQLRHKMAMATRNYLDKQGFL
                     EIETPVLIKSTPEGARDFVVPSRMNPGQFYALPQSPQMFKQLLMVSGYDRYFQLVKCF
                     RDEDLRADRQPEFTQIDCEMSFIEQEDILNTFEGLIRYLFKEIKGIEFKEAFRRMSWA
                     DAMRYYGSDKPDLRFGMSFVEIKHLTTGRSFNVFDQAEYVGAICAPASASYTRKQLDE
                     LTEYVKKPQIGAKGMVYVRCEADGSFKSSVDKFYTQDDLKTWAEACQAQPGDLILILS
                     GATQVTRKALSELRLEMGRRLGFCDKNLYVPLWVVDFPMFEWDEETARYYAMHHPFTS
                     PKPEDVPLLEKDPSQVRANAYDMVINGVELGGGSIRIHDSRLQADVFKILGFTKEEAT
                     QRFGFLMDAFKFGAPPHGGIAFGFDRCVSMFAGLDSIRDCIAFPKNNSGRDVMIDAPS
                     NIDQEQLDELHLKILTPKNKE"
     gene            35855..39079
                     /gene="carB"
                     /locus_tag="PLI37_00465"
     CDS             35855..39079
                     /gene="carB"
                     /locus_tag="PLI37_00465"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HOQ57522.1"
                     /translation="MNQDLNKVLVLGSGALKIGQAGEFDYSGSQALKALREEGIRSVL
                     VNPNIATIQTSEGIADTVYFLPVTPYFVEKIIEKERPDGILLAFGGQTALNCGTELYL
                     SGVLKQYGVKVLGTSVEAIMYTEDRDLFVKKLNEINLKTPVSQAVETMEAALKAADKI
                     GYPVMIRSAYALGGQGSGICTNREEFIALAQNAFSFSKQILIEESLKGWKEIEFEVIR
                     DKNNHCFTVANMENVDPLGIHTGESIVVAPTCSLDDTELNMLIELSKKCVRHLGIVGE
                     CNIQYAFNSETDDYRIIEVNARLSRSSALASKATGYPLAFVAAKLALGYTLDQIGEMG
                     TSNSAYIAPQLDYIICKIPRWDLTKFAGVSREIGSSMKSVGEIMSIGKSFEETIQKGL
                     RMIGQGMHGFVGNEGAEFDNLDQELSRPTDLRIFAIADALKKGYSVERICELTKIDPW
                     FVGKLKNIYDYTLVLKQYEKIEELPEDILLEAKRLGFSDFQIARFVENPKGNMEKDNI
                     RVRDRRKQLGVLPKVKRINTVASEHPELTNYLYLTYEGETHDIPYYPNEKSVVVLGSG
                     AYRIGSSVEFDWCSVNAIQTARNLGYKSIMINYNPETVSTDYDMCDRLYFDELSFERV
                     MDVIDLERPKGVIVSVGGQIPNNLAMKLYRQQVPILGTSPVSIDRAENRHKFSSMLDQ
                     LGIDQPAWKELTSLEDIKIFIEKVGYPVLVRPSYVLSGAAMNVCYDEDDLESFLKMAA
                     KVSKEFPVVVSQFLQNAKEIEFDAVAHNGDVVEYAISEHVEFAGVHSGDATMVFPAQK
                     IYFATARRIKKISRCIAKELNISGPFNIQFLAKNNEVKVIECNLRASRSFPFVSKVLK
                     RNFIDTATRIMLDAPYDKADKNAFDIDWIGVKASQFSFSRLHKADPVLGVDMSSTGEV
                     GCLGDDFGEALLNAMIATGFNIPDKKKGIMVSSGGSKSKVDLLDACIMLQENGYPIYA
                     TGGTQQFLKEHGVKAELVHWPDENNDKRQILNMISDRAFDLLINIPKNLTKRELTNGY
                     KIRRAAIDHNIPLITNARLAAAFIESFCSIDMNDIQIKSWQEYI"
     gene            39098..40504
                     /locus_tag="PLI37_00470"
     CDS             39098..40504
                     /locus_tag="PLI37_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOQ57523.1"
                     /translation="MGGFFGTISNSSCIKDLYYGIDYNSHLGTRRGGMATLYNGKFYR
                     AIHNLENAYFRSKFEPELLSFLGNSGIGVISDTDPQPIVINSRLGKFAIVTVACVNNI
                     KELEKDLLDSGRHFSELSSGQTNQTELIALIITQGKTFVEGIELVFQKVKGSCSMLIL
                     TEEGIIVARDKFGRTPIVIGKKDGAYAASSEPCAFPNLDYKTDYCLGAGEIVLLRPNN
                     MQQLREPNKRKQVCSFLWVYFGYPVSDYENINVDSVRYKCGLTMGNNDDSEVDFVSGI
                     PDSGVGLGLGYAEGKGVPYRRAIVKYTPTWPRSFTPSDPGIRKLVAKMKLIPNRSLLT
                     GKRVIFCDDSIVRGTQLSDNVSELYKYGAKEVHIRIGCPPLIYTCPFLHFTATKSDLE
                     LITRRIIQEIEGAHDKNLSEYSKTDSVKYRQLVEHIRARFDITSLKFNSLENLVKAIG
                     LPKESLCTHCFDGSSYFD"
     gene            40554..40628
                     /locus_tag="PLI37_00475"
     tRNA            40554..40628
                     /locus_tag="PLI37_00475"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40589..40591,aa:Pro,seq:tgg)
     gene            40890..41282
                     /locus_tag="PLI37_00480"
     CDS             40890..41282
                     /locus_tag="PLI37_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOQ57524.1"
                     /translation="MTHIKSLEQLTSKRDELKSNLSRLDDPNNSEKKVQIKVAMATCG
                     IASGARTTMDFLTAELSKRNIPSVVTQTGCMGYCYAEPTIEVKLPGKEAVIFGYVDTK
                     RADEIIEKYIKTGELTEGVIPRNYDTID"
     gene            41293..42792
                     /locus_tag="PLI37_00485"
     CDS             41293..42792
                     /locus_tag="PLI37_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOQ57525.1"
                     /translation="METPVNKPMQLRIALRNCGMLDPENIAESIVRDAYQALGKVLKE
                     YSPQECINILKQSGLRGRGGAGFPTGLKWEIASKSKSDQKYVVCNADEGDPGAFMDRS
                     ILEGDPHSVLEAMAICGYCIGADKGLIYIRAEYPLAIKRLKIAISQAMEWGLLGKNIL
                     GSGFNFEIKLCYGAGAFVCGEETALIHSMEGKRGEPTNKPPFPATSGFMKKPTNVNNV
                     ETFANIAPIILKGPEWFSSIGTKNSKGTKVFALAGKINHVGLIEVPMGTSLREIIYEI
                     GGGIKENKKFKAVQTGGPSGGCLTEKHLDTPIDYENLIAAGSMMGSGGMIVMDENDCM
                     VSVAKFYLDFIVGESCGKCTPCRVGNKRLYEILERITQGKGVMKDLDKLKNLSYIIKD
                     TALCGLGQTSPNPVFSTLENFYDEYYEHVVEKKCRAGHCKNLFHYVINEELCVGCAAC
                     KRCCPVHAITGEKRKPHYIDSNICIRCNECIEKCSFDAISIQLEPKILV"
     gene            42792..>43297
                     /locus_tag="PLI37_00490"
     CDS             42792..>43297
                     /locus_tag="PLI37_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOQ57526.1"
                     /translation="MEMIELSIDNKVLRVKKGTTILKAARKVGIEIPTLCHMELHNMN
                     IENKPGSCRVCVVEVDGRRNLAPACITEVTPGMVVNTHTVRVLNARKTIVELLISDHP
                     YDCLVCAKSGNCDLQSIAHKFGIRRVHFKGEKSTYRKDTSPSIIRDVDKCIMCRRCEM
                     MCNEVQTVG"
BASE COUNT        12176 a        10191 c         9399 g        11531 t
ORIGIN      
        1 taaccggcac gttcacaata gttcaccgct ttggtcaaat cgttggtttc ctgctcatcg
       61 atagttatga tacggtcggc ataaagctct ttcagtgagg gactcaggct gtccatatcg
      121 cccacaataa cagagggttc tctaccgaaa ttcaacaatt tttcggcagc tccatcgcag
      181 caaacaacat gttctgcctg gcgtaataag tgcagcaacc cgggttttcg ggggaactct
      241 ccgttggcaa ggatgatggt tgaaggcata actaaatctt aaaaacgaat ttcaacatat
      301 aatgtctggg agccaatata taataaccgg tttcccggta gggctcttct ccgctaagaa
      361 aagcgacttc ataaccccag gcattgcaca agggctggtt acccagtaaa ttgtttatat
      421 aaaagcccaa ttcaaagtcg ctgttgccaa ctaaacaccc tgaatatacg gcagaaaagt
      481 taaactgggt ataggccggt aagcgaaatt cctcgtgctg aatatagcta aactgctgct
      541 ccgctatata ttttcccatt aagctcaatt gtaaagaggg tacgggctga tactgaagac
      601 gggcagtagc aatacatgcg ggagaaaaag gcaaatcggc cagggaataa tagctgctga
      661 tctgccggct ttggtcaagt atctcattcc agtccgaggc attatcgtag gtactgtaat
      721 acaggctaaa atctttaacc ttattggtac tcagactcag attggcatcc aactgcaaga
      781 cggaaaaggg cctgatgccg gcactcagtt ccattcctcg acggtaactg tagggcacat
      841 tgctcataag tttataaccc acctcgttta gtttacctgt aggtatcaac tggttgtggt
      901 attccatatc gtacagattc agggccagca tccattttgg tttattgacc gaataaccca
      961 gttcaagatc gtacagcctt tcgggtaaga cccgtcggct gcctccttta acggcatcct
     1021 tcaagtctgc acgggtgggt tcccgattgg caattcctgc cgaaagatat agtttccgac
     1081 tctggtctat gcgccagttt acacccattt tgggattcca gaactgccat tgatgcgatt
     1141 ggcttaaatc cagtaaatca tcgtcatcaa ttccctccag ttcatatctc acattacggt
     1201 attgcaggtc ggcatacaaa aaaagtttat tccgaagagc atattcagcc ttaacaaata
     1261 aattgccgtc tgttttttcg ccaaaattac gcgcccactc atagttagca gggatgctgc
     1321 tgttgtgttc cagccataat aaacgggcgt agtgaacccc ttggtaaaaa gagtacatgc
     1381 ctccgccctg gagcctgaaa gctcccggtt ggtagttcaa attgaacaat ccggtataaa
     1441 aaccgttggc catgaacttt ccccggatat gatcgctgcg gtcgtactct attccgtcaa
     1501 tcaattggtt gcttaaacca agggacgaaa actttctgtt ttgtttatac tgttcatagt
     1561 agccgtaccc gtccgtgtaa ttcaatccgg tattcaacaa aagcgtttcc gtaagttccc
     1621 gggaataaaa gagctgaata tggtgctggc gataattgtc gctttcgttg ccgtaataca
     1681 agccgtcctt gatggctcct gccggattat aggccggatt caaagcaatt tgttctttgg
     1741 aagctccttc ccaggttata cccgtatgct gattgccgta tatgtaattc atgctcagct
     1801 gagctttttc ccaggacttg cccagcgtgc cgtagagcga ttgatgacga ctccagccgt
     1861 tgcgcaaata accgtcggca tttaatgctg tatagcgcaa ttgcatctgc acccctccgg
     1921 gcaggcgtga agttgccata gaaacactct gttcaaaagt accgtaacta cctgccgttg
     1981 tggaagattc tatataattt tcagctgccg ggcgctcgct cagtaaattg acagaagctc
     2041 caaaggctcc ggtaccattg gccgacacac ccaccccacg ctgaatttgt atgctttgca
     2101 gcgatccggt caggttggga atattcaccc aatacacttc ctgcgattcc gggttgttca
     2161 tgggtatccc gttcaaactg aaatttatgc ggctggcgtc cgttcccctg attcgcatgg
     2221 ccgtgttgcc ggtgcttgtc ccgttttcgg cggtatgcac cagcgaggga gtcatccaaa
     2281 gcacctgggg caatgttgaa actacgttca gagtgcgaat ctcggctttg ctcaggtttt
     2341 gatgagttac cggggtgttt tcccccgcat aggaggcaat taccaccaca tcgtttaaat
     2401 caagttgtct gatactgtct gtttttgcgg ctgccatgga atgaacgaca aaaatgcccg
     2461 ccgcaaggca taaaaaaacc cttttcatgt ttttcttctt aatagtgaaa catcaaaagg
     2521 ggtgtaagag aaaacatttc ccgacggtag cattacctac atccggtctt gggtataatc
     2581 tcagatcact caaagcatag tagttttaaa gtgtcgttct gtccgactta aaataaaacg
     2641 acaaaaagaa ctgtatgccg agggaacacc cctaaaaaat ccacgcaaat gtaagcaata
     2701 tttttgtctg cccctatttc ggggcatatt ttaaaaataa ttatctttgt agacttatta
     2761 aacacaaaga ttatgggaaa agttcttatc atcggagccg gcggagtcgg aagcgtagta
     2821 gcatacaaag tagcacaaca tccggaagta ttcagcgaaa tcgtgttggc cagccgtacc
     2881 aaggcaaaat gcgataagat tgctgccttt gtgggcaaca aacgtattca gaccgatcag
     2941 gtggatgccg attctgtaga gcagcttgtg gcattattca aaaaacaccg accggaattg
     3001 gtcattcatg ttgccctgcc ctatcaggat ttaaccatca tggatgcctg ccttgcctgt
     3061 ggcgttcatt acctcgacac agccaattat gaaccaaaag acaaggctca ttttgagtat
     3121 tcctggcaat gggcctacaa ggaacgtttc gaaaaagccg gtttaacagc cattctgggt
     3181 tgcggttttg atcccggtgt aaccggtgtg tggactgctt atgccgccaa acatcatttt
     3241 gatgaaattc attacctgga tatagtggat tgcaacgccg gcgatcatgg taaagctttt
     3301 gcaaccaatt tcaacccgga gataaatatt cgtgaggtca cacaaaaagg caggtattac
     3361 gaaaacggcc aatggattga aaccgaacct catcaaatac ataaaaccct tacttatccg
     3421 gaaatagggc ccagagaatc ttacctgatt tatcacgaag aactcgaatc gctggttaaa
     3481 aatttcccga ccatcaaacg agctcgtttc tggatgacct ttggagaaga ataccttacc
     3541 catttgcgtg taatccaaaa catcggaatg gccggcattg agcctgttat gtttaaggga
     3601 atggaaatta tccccattga gtttctgaaa gccgtcctgc ccgaccccgg cgaactggga
     3661 gaaaattaca ccggccagac atccataggc tgccgtatcc ggggcatcaa aaacggcaag
     3721 gagcgaactt attatgttta tcaaaattgc agccaccagg ccgcttacaa agaaaccggc
     3781 gctcaggctg taagctatac taccggcgta ccggccatgt gcggagctat gatgtttatg
     3841 aaaggtttgt ggcgcaaacc cggtgttttc aatgtggaag aattcgatcc ggatcctttt
     3901 ctgaaggctt tggccgaaaa cggtttaccc tggcatgaac ttttcgatgt ggatctggag
     3961 ctctaaatca tcgtattcaa agcataaagc ttcggttata caattttgcc taattctatt
     4021 acttccaggt tttcgatatt accctcgttc aattcgagac gcataagcgt acgaatggtg
     4081 tgaaaaccct gtttgccggc ggctcccgga ttgacacaaa gcatatcgtg tcgacggtcg
     4141 aacatcactt tggctatatg ggaatgtccg caaacaaaca aatccggttt gtgatgtttg
     4201 atcagagcca gtgctgagga atcgtaacgc cccggataac cgccaatatg cgtcatcaac
     4261 accctgacct gctctaattc aaaaaacaga tgggaactaa aattatacct taagggataa
     4321 ccgtcgcaat tgccatacac ggctcttagc ggcctaatgg cttcgaggcg ttgggtcagc
     4381 aaaacagaac caatgtcgcc ggcatgccag atttcgtcgc aaatctccag gtaaggaatg
     4441 tagcgttcat cccaatagtc gtgagtgtcc gataaaatag caattttctt catgattaga
     4501 attgattaac agggagttga gagcgtatct tccaggaagc aggataacgg ttattcgccc
     4561 taattcccaa attgtttacc caacccaggc cgacacggcc gtaagtcacc agcacctgac
     4621 cctgcgggca atcctcgctt aactgcaaac tctctctctt caaataagcc aaagcttgtt
     4681 gacgatgcag ttcatacgaa gggaaatgtt tgccggcctc aacggcggta gaaagagcca
     4741 gtgaaacatc gggtataaac tgattgcctt tatactctcc caggtacaga ccggcacgta
     4801 ctatgcgcag ggaatggcta atccgggcca gaaaagcggc gtgttgtgac ggaaatgccc
     4861 tgtaaacacc ccatggcagg agactgattt cgtaggcttc gggctcgtca aacagagagt
     4921 tcaaataggt aagtttcttt ttgtccacaa cttcgttcac aaattttccg gattttttcc
     4981 gggagggtaa tttcttttta ccctttttgg gtttaaaaag ctcgtggaaa tctgcttgcg
     5041 gataactttt caaaccggtc tgtaggtttc ctgcaagtat ctgtgagtct gctaggccgg
     5101 tgcgtgagtt tacagcgtcg gtgcgtgagt atgctgcgcc ggtccctgaa tttgctgcaa
     5161 gactctcctc tttttgaagc aaagctaaaa agaaaccttc tcctctggtt ctgtgtggga
     5221 aaaaacgata agccggaatg tcggctccga tggcaggatg aatactccag tcgggattgc
     5281 attctacagg cagaacgcgg gcacctagat tttcggctgc ccacaacagg tttttttcgt
     5341 tttcctctgt attgtaggta caagtactgt aaatcaggca gccgcctggc cgcaggctgt
     5401 tccagcattg agccaggata ttacgttggc ggagcgcaca gccgagaaca ttgtcttcgg
     5461 accaggctgc cagagcctcc ggttctttgc gaaacatacc ctcgcccgag cagggcatat
     5521 cggccaagat caaatcaaaa gcatgttcaa attcggcaaa atcggccggt tcgtttttcg
     5581 taacgacaac ctctgcctta ccccatttta cgaggttttc ttccagtatc acagccctcg
     5641 aaggaacgat ttcgttggcc agcaacaggc tgccctcagg tagcaccgag gcagccagag
     5701 tggatttacc tcccggggct gcacaaagat ccagcatccg cactgcccgg ccggacatct
     5761 tcatcgcctg caaagcagca tgaatggccc gggcagtgaa catagaggat gcttcctgaa
     5821 cgtaatacag gccgcaatga aaaaacggat cgaaagtaaa ggatggacgt tgttccaaat
     5881 aataggcgtc gggacaccag ggcacagcac tctgaacttt tgcaggcaaa cagttaagcg
     5941 ttttctctcc ggagcattta gccgtattta atctcaaact gacgggaata tcttcttcca
     6001 aagagcgcat aaacgccgcg gattcagcgg gcaatagccg gcgcatctgc ctgacaaaag
     6061 cgggatctaa attcatggtg ttcagggaaa aaactttttt gcctgctgcc aaataacttc
     6121 catttgatcc aaactcgttt ccttaagcga caaacccttt tttagtgttt cctcctccag
     6181 gtaattgaaa cgttcacgaa actttttgtt ggtgtgttcc agggcgttgt cgggattgat
     6241 tccgtagagg cgggctacat tgatcaggct gaaaagtaaa tcgccgaatt cgtcttccat
     6301 gccgtctttg tcgtcttttt ctatctctcg ttgaaactcc ctgatttcct cctgtacttt
     6361 agcccatacc tgtctgcgat cttcccagtc aaagcctact cccgcggctt tatcctgcat
     6421 tctgtatgct tttataagcg agggtaagga agaaggcact ccggccaata ccgaaccatt
     6481 gccgttcttt tctttgagtt ttaatttttc ccagtttttt tcgacctcgc cggcgtcttg
     6541 tacgctgact gtgccaaaaa catgaggatg cctgaaaatc agcttttctg cgagcttatc
     6601 acatacatcc tcgatatcaa acaattgctg ttcctcggct atttttgaat aaaaaactat
     6661 atgcaaaagg atatcgccca gttctttaca aacatcggga tggtcttttt gcaacagggc
     6721 ttcgcttaat tcgtagcatt cctccagcgt aagagggcgc agactttcga aggtttgctc
     6781 tttgtcccag gggcatttca aacgtaattg gtccaaaatg tcgagcagtc gcccgaaagc
     6841 ctccattttt gattgtctgt cgtgcataaa aggttaaatg tcgagatcga agagcgcacc
     6901 ggcctttact ttgacagcga tgacggttcc ggacagaaat tcgaaatgta ttccgtcacg
     6961 aaggcaagcc ggttttttgt tattactgct gatctttgcg tataaaatgg cttcgaaacc
     7021 gcactctata agtttaaaca cctcattcgg cttgaaataa acgctttcta cactaagttg
     7081 tttgctgttt gtctttgtgt ttacttctgt taagcaaaaa ggaacaaact cttcttcgcc
     7141 aaactggcgt acttttatca aaaaagaatt cactccgcta aaatcaactg tatcctctga
     7201 caagcgcatt ttagaatttt tcaattcagc cttttcaacc acagaattga aagtctgccc
     7261 cacttccgat aaaaaggcct caatatccag ggcatcgatg gaatcgcttt tcgcaatggg
     7321 tctgtaatcc aagaaggcat cctgattgga tttgtgattt ccggaggatg ccattttcaa
     7381 tgcttccctc tgattatcag cgtcaaaaaa ggccatctta aattcaatat ccggcccctg
     7441 aacatcaata gtaaatccac ccaccttggt gcaggctgta aaaaaacaaa acaatacaag
     7501 agcaattatt cctctatagt acttctgatg tgctattatc ataaggtcaa attgaattat
     7561 ttcaatacaa atgtacaact tgttacattc gttacgctaa ttgttataca atattaacag
     7621 atcatccctt gcccggttgc ccactaaaag taataaaaat tacttgggca tagaaaaaaa
     7681 tagttctacc tttgcagcct taaaatctgt acaatgcctg aatccgataa acacgggccg
     7741 gggcatttaa ccaaacaaga tttctgacta ttatggaact cccttctaag tacaatcctg
     7801 ctgaggtaga aagtaaatgg tatcaatatt ggctgcaaca caacatgttt cattcgactc
     7861 ccgacgaaag agaaccctat accattgtta ttccgccgcc caatgtgacg ggagtgcttc
     7921 atatgggaca tattctgaac aacaccatcc aggacatatt gattcgcagg gcccgaatgc
     7981 ttggtaaaaa tgcttgctgg gtgcctggaa ccgatcatgc ttccatcgcc accgaagcca
     8041 aaatagttga aaagctcaaa aacgaaggca tcaaaaaaac tgatattggc cgagaagctt
     8101 ttatagaaca tgcctgggaa tggacacaca aacatggagg tattatactt gaacaactca
     8161 aaaaactggg agcttcctgc gattgggatc gtacctgttt taccatggac gatgtccgtt
     8221 cggaaagcgt attaaaggtt tttattgatc tttttgagaa agggctcatt tacagaggtg
     8281 tacgcatgat caactgggac cctaaagccc aaacagcttt gtcggacgaa gaagttattt
     8341 acaaagaagt acagtccaaa ctctattacc tgcgctacaa agtgcagggt gaagatcgtt
     8401 acgcaactat agccaccacc agacctgaaa ctatattggg cgatacggct gtctgcatca
     8461 accccaagga cgaaagtaag gcctggctca ggggtaaaaa agtactggtc ccgctaatcg
     8521 accggcctgt acccatcatc gaagacgaat acgtcgacat agaattcggt accggctgcc
     8581 tcaaagtgac tccggctcac gatgtgaacg actacatgct gggcgaaaaa taccgtctgg
     8641 aatctataga tattttcaat aacgacggta cgcttaacaa acacggagcc atgtacgccg
     8701 gcatggatcg cttcgaggtg agatctaaaa ttgaaaaaga tcttaaagca gccgggttgc
     8761 tcgaaaaaat agaagactat accaataaag taggctattc cgaacgaacc gacgaagcca
     8821 tagagcctaa gctttcgatg caatggtttc tgaaaatgtc cgatttggcc aaacccgcct
     8881 tagaagccgt tgaaaaggat gaaataaagc ttatcccgcc aaaatacaaa aacacctacc
     8941 ggtattggat ggaaaacgtc aaagactggt gtatttcccg tcaactttgg tggggacatc
     9001 gcattccggc ttattacctg cccgaaggag gttttgtagt agcatccact cccgaagagg
     9061 ctctcgagaa ggctaacgcg ctgcgcatga aaaatgtttg cccctgcgga tgcagcggat
     9121 tgaaacccct taagcccgaa gacctgcgtc aggacgaaga ttgtctggac acctggtttt
     9181 cctcctggct atggcccatt tccgtttttg acggaataaa tgagcccgac aacaaagaca
     9241 tcaattacta ctaccccacc aacgatctgg tgacagctcc cgatatttta tttttctggg
     9301 tggcccgcat gattatctcc ggttttgaat accgcaaaga ggcctgtttt aaaaatgtgt
     9361 acctcaccgg aatagtccgc gacaaactgg gacgcaaaat gtccaaatcg ctgggcaact
     9421 cacccgatcc catcgaattg atggataaat acggtgccga cggtgtccgt atggggatga
     9481 tgctggctgc ccccgccggc aacgatgtac tttttgatga agccttgtgc gaacagggtc
     9541 gcaacttcaa caataaaatt tggaatgcct atcggcttat aagcggctgg aaggtagacc
     9601 ccaaactaga tcagccgaaa gcctctgctc tggccgtaga atggttcgaa gcacgattag
     9661 ggaaaacact ggctgaaatg gacgatttgt tcgacaaata ccgcttgtcg gaagctatga
     9721 tgtgtgttta caaactcttc tgggacgaat tttcttcctg gtttctcgaa atcattaaac
     9781 ccgcctatca gcagccgatc gacagcataa ctcataaggc tgttttcggt tttttcgaac
     9841 aactctgtct tttgttgcac ccctttatgc cctttattac tgaagaactt tggcagcatc
     9901 tggaacaacg agaacccggt gaaagcctga tgttacaaca acaaaggaag ccaaccgcct
     9961 acaatcaggt aatattggac aaatttgaac tggttaaagc tgttgtttct ggcattcgta
    10021 ccattcgctt ggaaaaaaac cttcccgtca aaacagctct ggcgcttgaa atacagggta
    10081 aacacgatgc aagttttgac agccttatag taaaaatggg caatataagc agcattaccc
    10141 aagtgagtgc taaggcttcc gatgcggcct ctttcctggt aaatacaacg gaatatagtc
    10201 tcttacttgg cgctttgatc gatacggaag aagagcgcaa aaaaatgaat aatgaattgc
    10261 aataccagga aggctttttg caaagcattc tcaaaaaact gaataatccc aatttcatca
    10321 gccgggctcc cgaagcagtt atagaagccg aacgtaaaaa aatggccgat gccaaaaata
    10381 aaatccaggc catcaaagac agcctggatt cacttgaaag caataaatga atcagctaat
    10441 gtaaccctga tccatcatac tttttgcaac cttcataaaa ccggccacat tggctccttt
    10501 cacgtagttg atatagccgt cggcttcttt cccgtacaaa gcacattggt cgtgaattgc
    10561 cgacatgatt tgccgcagct tctggtccac ttcttcggca gtccagctta aatgcatggc
    10621 attttgagtc atttccagac ccgaagtagc cacaccgccg gcattgacgg ccttgcccgg
    10681 agcaaagaga attcttgatt ctacaaaagc gtcgctggct tctgcggtac aacccatatt
    10741 cgaaacttct cccacgcaaa taacgccatt gcttaacaaa tttttagcat cctgaaggtt
    10801 taattcgttc tgagtggcac aaggcaaagc aacatccacc ttgcgttccc agggtttttt
    10861 gcccgggata aattcggcct tgaatttttt ggcataaggc gctatcacgt cgttgttact
    10921 ggcacgtaac tcgagcatat aggctatctt ttcgtcgtcc aagccggctt catcatagat
    10981 gtaaccgtcg gggccagaca atgttaccac cttggctccc atccgggtgg cctttaaagc
    11041 ggccccccaa gctacatttc cgaagccgga tatagctacg gttttacctt cgatgctcat
    11101 acccttggtt tccaacattt gatttacaaa atacagacaa ccataacccg tagcttcggg
    11161 acgaaccaaa gaaccgccga aacccaaagc tttacctgtg aatacaccgg agtttactct
    11221 ggccagtttt ttatacatgg catacatata acctatttct ctgccgctca caccaatatc
    11281 accggcagga atatcggtat caggcccaat gtttcgccaa agttccaaca tgaaattatg
    11341 gcagaatcgt tgtacttcgg ctgcagactt acccttggga gagaaatcgg agccaccttt
    11401 ggctcccccc attggtaatg tggtgagcgc atttttgaaa gtctgttcaa aacccaaaaa
    11461 tttcaatatg gacgggttta cagaagcatg aaaacgcagc ccccccttat atggtccgat
    11521 ggcgttgtta aattgaatcc tgtaacctgt attcacccgt atatttccgg cgtcatccac
    11581 ccaagggact ttgaagatga aaatccggtc aggctctact atgcgttcca gtattccggc
    11641 tttatcaaat tccggatgtt cattgtaaac tttttcaata cttgatatta cttctcttac
    11701 tgcttgcaga tactcgtgct cacctggatg cttcatctcc aggttagcca taaaacgatt
    11761 aacttccatt gttttagatt attttttagt ggtttaaatt tgggtttcaa atattcactc
    11821 aaaaaaaaga tgatataaaa tcacagttcc ttgaaattgc catttctcta tgtattacaa
    11881 ttagtctgta atccggttat tttttctttt tcaagacgga aaaaacaaaa taaccaatca
    11941 acagagcata catcaatagg gacaacattt cagccccttt ggtgtttgcc cacgatgtca
    12001 gcgtccagtc catgcctcca aaacgcaaag ccgcactgac aactcccatt aaggcgccac
    12061 ctgcaataaa gccggaagct atcaatgtcc ctttttcgtt tctggccgta ttcagtttgc
    12121 tgtctttact acgcccggaa acaaaccaat tgatcaagcc gcccacgacc agaggaatat
    12181 tcagttgaaa aggaataaac attcccagag caaaagccag ggcaggtaca ccgaaaaaat
    12241 tcaggatcag tgaaatcagg gcacctgtag cgtacagcca ccaaggtgcg gccgttccag
    12301 acattaaagg gtctataacg gcagccattg cgttggcctg cggagctacc aaggcgtttt
    12361 cgcccacaaa tccataggtc ttattcagaa tgataagcac ccctcccact gttaatgccg
    12421 ataccaaggt gcccagaaat ttccagcttt gctgaacggc gggggtcgat cccagccaat
    12481 aaccgatttt gagatcggta ataaaacctc cggccataga caaggctgta cacactactc
    12541 cacccattat caaggctgaa actatacccg tagaaccttt aagcccaacg gctaccataa
    12601 tgaccgaggc cagaatgagg gtcatcaatg tcataccgga gacaggattg gttccgacta
    12661 tggcaatggc gttggcggct accgtagtaa acagaaaagc aattataccc actatcagaa
    12721 ttcctactat ggcgtgcaag agatttaagt gcatcacacc gaaaaagaaa aagacaaata
    12781 ccgccagaag ggccagaacg ataccgatca atatgatctt catcggtaaa tcactagagg
    12841 ttctggcttg agtaacctgc tcagctgttt tggctccctg acctttcatc tcacgggctg
    12901 ccagtccaag ggcgcttttt ataaccggcc aagatttcac tatacctatt attccagcca
    12961 tagcaatgcc gccaatacct atatgtctgg cgtagctgct aaaaataact tcgggcgaca
    13021 tacctgctac accatcactg atggccggat tgagaaactg caaaaaagta tccccaaaca
    13081 gaggcagcaa agggacaaaa acaaaccaaa tcaaggccga acctaggcaa atgacagtgg
    13141 cgtatttcaa gccgacaata taacccagtc ccaatacggc agcgccggta tttatgctga
    13201 aaaccaattt aaacttatcg gccatcatcg taccaaactg acttaccctg gaagtgaaaa
    13261 cttccgtcca agagccaaaa gtggctacga caaaatcgta gagtccgcca attaatccgg
    13321 atacgacaag aggttttgcc tgattggctc cctgctcacc ggatattagc acctgagtgg
    13381 ttgccgttgc ttcggggaaa gggtatttgc cgtgcatttc ggacacaaaa tacttgcgaa
    13441 acggaatcaa aaacaaaata cccaaaacac ctcccagcag cgaactcaaa aagacctgaa
    13501 tgaaattaac actcatatca gggtatttgg cttgcaaaat atacagagcc ggcaaagtaa
    13561 aaatagcacc ggccactatc actccggaag aggagcctat ggactgtatc aatacgttct
    13621 cgcccaaggc attttttcgt ttggtcagac tggacaaacc aacagcgata atggcgattg
    13681 gtatggccgc ttcgaaaacc tggcctattt tcaatcccag gtaggcagca gcagccgaaa
    13741 acaaaatggc catcaaaaca ccccaactta ccgaccatac agtggcttcg ggatattttc
    13801 tgccgggaga catcacagga tgatacaact caccctcttt caactctctg taggcatttt
    13861 ccggcaaagc agttaccggc ttgttttctt ctgttttact catatcttag aattttaaag
    13921 ctgtccgttc cacatcttcc aggctgcttc ggcctgaatt ttcaacatgc ctgccccgtt
    13981 cattacacgg gctccggcct gttgacccag gctgagaaaa cgggtgagag cagggttgta
    14041 tactaaatcg tagagcaaat gtccccgccc caggtagcga taaggcaaat cgggacaaga
    14101 atccgtatcg ggagccatac ctaacggagt ggtgttgaca ataatcttaa aacgatccat
    14161 tagttccttg ttcagcatgg cgtaggtata ttcagcctta gcggcatcgc gcgatacgaa
    14221 agaagtttct atacccattt gctgcaatgc ataataaaca gctttggaag cgccgccggt
    14281 gcccaatatc aaagccttga gttgttgagg gcacgatgct tttaaacgct caatctcggg
    14341 cagaatgcta tctctgaatc ctatataatc gctgttgcgg ccaattaacc ggatttttcc
    14401 ctctttgatt tcgggtacaa tcacatttac cgccttaatg gaggccgctt caggacccaa
    14461 ttcgtccaaa aagggaataa cgctttgctt ataagggatg gttacattca aacccgccaa
    14521 ggccggccgg gactgaatca aagacggaaa ttcttcgatt cttttcaagg gaaaattttc
    14581 ataaacagcc tcaattcctt ccgattgaaa tttttcgtta aagtagacct gtgaaaaaga
    14641 ataagtcaaa gggtagccaa tcaggccaaa ttctcgtttc atttctgttc ctccccaata
    14701 attttattga ataactctct tgcatgaaca tcactaagca aggccagttt taaagcatca
    14761 tcggcctgta gcatgtcccc attgcgggca taagccagcg ctttgtataa gtgtatgttg
    14821 ggaaaagtag catccagcaa ttcagcgtcg gaataatact tcagcgccaa ctcaaacttt
    14881 tgctgatcgt aatacagatt accaagagca aaaagtacct cggcttgcga gggatcaagg
    14941 cttaaagcat gcaaataggc tacaatggct tgctcattct tatccatatc gacaagcacc
    15001 tcggccagat aaacccagat ctcggcttgt ttatcgtcca ttttgatggc tttttccaga
    15061 taccgaaggc tttgcttgta atcttttaat tccatatagc aaatccccat tccggtatat
    15121 gcctgtaaat taccgggatt agtggtcaat gcatgttcgt accattctat agccgattcg
    15181 tagtgttctg ctttttcaaa agcctcacct acaaacacca ttaagtcgtc tatgctattg
    15241 ccttcggcag agggaaggct ttcttctatg gctgccagca agtaatcaag gtaaaatttg
    15301 gctgcgtctt cgtaatgttc catctgaaaa caggcatgcc ccatctgaag caccgcaggc
    15361 ataaacccgg gattgcttac caacgcaaaa tcgtaagctt tgcatgcttc ttcaaagcgc
    15421 tcctggctaa acaatatctg ccccaggttg aaccagtttt cgtaagaata aacgtccacc
    15481 tcgagcaaca ttcggtaata atgttccgag gcaggcatat cgttcaattg ttcagcacaa
    15541 aaagcacctt ccgacattaa atcagtgttt tcgggattgt gttccaggcc tagccgtaca
    15601 aaatccaagg cttccttata cagattttcg gcactcaata cactcactgc ttcaagacaa
    15661 gccaaatcct tatcgtattg ttctctggct accatatctt tgagcaaagc cagaccttct
    15721 ttttttcttt taagctttaa ataagcggca gcgcgtacaa aatcagtctc ttcctgctca
    15781 ggtaaacggc ccagcagttg cagggctttc atcggttcac ccgtttccat gtacaaaatg
    15841 gcacgtttca gatgaataac cgtactgttg ggatgaatac gcaaagcaga attgaccact
    15901 tccgagaact tgttggtgcg accccggtcc aggtaataat cggcaatacg atcgaattca
    15961 tctacgtcaa aatactgcga caaaccgttg gagcgttgtt gttcataacg cttaactaat
    16021 gcacgtatac tatcgtcctc tcttgacttc attcggattc aaaaaatata ttgccttatt
    16081 ttttagcgat cttgctccag gtgtccctga ggcctacagt ccggttgaaa attaattgac
    16141 cggggcggga atcggggtcc agattgaaat agcccatacg cagaaattga aaatgatcca
    16201 gtggacgggc atcttgcaga gaagcctcca cacggcaatc tttcaaaacc ttgagcgaat
    16261 cgggattcag catgtccatg aaatctctct ctttgtcttc ggccggattt tctaccgtca
    16321 acaaacggtc gtataaacga acttctgccg gcaagctgtg ctcaacagta acataatgca
    16381 gggtggcttt taccttacgc ttgctttccg gacctccttc tttggtcagg ggatcgtagg
    16441 tacaataaat ctcctgcacc tggccatcgg cgtctttttt acaacccgta cattgcacga
    16501 tataagcatt tttcaacctt acttcctgtc ccggcgtcag tcgatagaat tttttaggtg
    16561 cttcttccat aaagtcatcc ctttcaacat acaattcacg ggcaaaggga attgagcgac
    16621 tgcctgcgct ttcatcttcg ggattattga tggcttcgtt ccattccact ttcccttcgg
    16681 ggtaattggt aataataagc tttacgggat tcaatacggc acttactcgg ggagcaatct
    16741 tgttgagatg ttcgcgtacg gcaaattcca acaagctttg atctatcact ccgtcaaatt
    16801 tcgtataacc tatcctgtct ataaaatttc gtatggattc gggcgtatag ccgcgcctgc
    16861 gcaaaccgca aagagtaggc atgcgtggat cgtcccaacc tttgacaaaa cccccttcga
    16921 ccaattgcag caattttctc ttactcatta tggtgtaggt tatgttcagg cgattgaact
    16981 caatttgcct gggccggtaa tcgccctctt gcaggtgatc tataaaccag tcgtagagcg
    17041 gccggtgtat ctcaaactcc agagtacaga gcgaatgagt aataccttca aaataatcgc
    17101 attgtccgtg agcgaaatcg tacaagggat aaatattcca tgtattgcct gtgcggtggt
    17161 gggcttgttt taagatacga tacatcaacg gatcacgaaa atgcatattg gaatgtgcca
    17221 tgtcaatttt ggctctcaaa accctgctgc cctcgtcaaa ttcgccggcc tgcattctca
    17281 aaaataaatc caggttttct tcggcagatc tgttgcgata aggactattg accccggggc
    17341 tggtgggtgt gcccttttgc tgggctatca cctcggcaga ctgatcatct acataggcca
    17401 aacctttttt gatcagttcc tgtgcaaact gaaaaagttt cccgaaataa tcggaagcat
    17461 aaaacaaatt atcccagcta aatcccaacc aacgtatgtc ttccataatg gaatcgacgt
    17521 attcaacgtc ttctttggtg ggattggtat cgtcaaaacg caaattgcag gtgcctccat
    17581 atttttgagc aatcccaaaa tccagacaaa tagccttggc atggccaata tgcagatagc
    17641 cattgggttc gggtggaaaa cgagtcgcaa ttctccctcg attttttcct tcctttaaat
    17701 cgttttcaac aatctgttct ataaaattca ggcttttttt ggagccttca ctgttggtgt
    17761 ctactactgt catgttatta aaaaatggtt ccaaagatac tgcctaattt tgcaagattc
    17821 aaatgagcaa caagaaacta tgctccaaaa acatgtttta taatataagt ttttttttgc
    17881 ctgtgtgcaa aaaaagttca acctttgctg ccgttaacct aacacaatct tttttacctt
    17941 tgagactaat acctattgaa aactatatga agtccgcctt gtgaaaggcg gacttcattc
    18001 tttttagcct ttccgctgca aaagtaatgt ttgtttatca aatatgaacc ttcggctcat
    18061 caaaaaataa tttgtatatt cgcaaaattt ttaaacctca aaaatacatc tactatgaaa
    18121 gcattcatgg acgaaaattt tatgttggaa aacgaagtat ccgaacagct tttccaccaa
    18181 cacgccaaaa atctgccgat tattgattat cactgccacc tgaaccccga atacattgcc
    18241 aaagatcaca aattcgacaa cctgggacag atctggctcg aaggtgacca ttataaatgg
    18301 cgtgccatgc gaaccaacgg aattgatgaa cgttattgca ccggtaagga taccagcgac
    18361 tgggaaaaat tcgaaaaatg ggccgaaacc cttccctata caatgcgtaa tcccttgtat
    18421 cactggactc acctggaact gaaaaccgcg tttggtgtga ataaaatact caaaccggaa
    18481 acagccaggg aaatttttga cgaatgcacg gccaaactgc gtactccaga attcagtgca
    18541 cgaggactga tgcgaaaata taatgtcgaa accgtctgta ctaccgacga tccggtagat
    18601 agcctcgagc atcacattgc tctcaaaaaa gagggttttg agattaaagt cttacccacc
    18661 tggcgtcccg acaaagccat ggcagtagaa gatcccaaat cgttcagagc ttacgtggaa
    18721 aaacttgccc aggcagcagg aacgaaaatt tcccgttaca acgatttgat tgctgctctg
    18781 agaatacgtc acgatttctt tgcttcggtg ggttgtaagc tcagcgacca cggtatagag
    18841 gaattttatg ccgaagacta tactcaggca caaattgaag ccatttttaa taaggtatac
    18901 ggaggcaaag aattgtctca ggaagaaatt ttgcaattca aatcggccat gatggtggaa
    18961 tttgccctga tggacgccga aaaagactgg acacaacaat tccactatgg ggctattcgt
    19021 aacaacaatt cgagattgtt tgcccaatta ggcccggata caggttttga ttccatcgga
    19081 gacttcaatg tcggtaagaa tatgtcaaaa ttcctgaatc gtctggacaa aaacaaccaa
    19141 ttggccaaaa ccattatata caacctcaac ccacgtgata acgaattaat tgccaccatg
    19201 ctgggtaatt tccaggatgg ttctgtagcc ggcaagattc aatttggatc gggctggtgg
    19261 ttcctggatc agaaatacgg tatgatcgcc cagatgaatt ctttgtcggt actcggctta
    19321 ttgagtcgtt ttgtcggtat gctcaccgat tccaggagtt ttctttcgta cccgcgtcac
    19381 gaatattttc gtcgtatcat gtgcaacctt atcggtgttg atgtggtgaa aggtgaaatt
    19441 ccttacagcg aaatggactt tctcggtaag atggttgaag gcgtgtcgta ctacaacgct
    19501 aaaacatttt ttaaatttta aacgacattc ataaacggct gcaatgaaaa ttttctttac
    19561 cttgcggccg tttattttac aacgacagat taaatgagta aaagagtagt tactttcggg
    19621 gagattatgt tgcgtttagc tactcccgga tacttacgtt tcagccagac ctcgagcctg
    19681 actgccactt ttggcggagg cgaagccaac gtggccgtat cgctggccaa ttacgggatc
    19741 aaagtagatt ttgtgagccg tttgccccta aacgatatgg caacctcttg cgttatgaac
    19801 ttacgaaaat acggggttgg caccgatcac ctgatttacg gaggcgagcg tttgggtatt
    19861 tattttttgg agaccggagc tgtggcccgt cccagcaaag taatttacga cagagcgcat
    19921 tcttctatcg ctgagattca gcccggcatg atcgactgga gaaaaatttt caaaggtgca
    19981 gattggtttc actggacggg tattacgcct gccatttctc aaggagctgc cgatgcatgt
    20041 ttagaagcga ttcaaatagc caacgagatg ggagtaactg tttccacaga tttaaatttt
    20101 agaaaaaatt tatggaaata cggaaaatca gcgtcggaag taatgcccaa attagtagcc
    20161 ggatgcgacg tgattttggg taatgaagaa gatgccgaaa aagtattcgg tatcaagccg
    20221 gaaggttttg aagtagccca taccggtggc gaagtcaatg ccgctgaatt tgagtcggtt
    20281 tgtaagcaat tgatggctaa atttccccgg gcaaaaaaag taattattac cctgagaggc
    20341 tccatcaatg ccaatcacaa tacctggaga ggatgtttgt attccgacgg atcattgaaa
    20401 atttcaaaag cttacgacat cacgcacatt gtggacagag tcggcggagg cgattccttt
    20461 atgggaggac tgatctacgg attgctgacc tataaagagg atgatcaaaa ggcattagaa
    20521 tttgctgtgg ctgcttcctg cctgaagcac accatttacg gagatttcaa tatggtatct
    20581 gtagccgaag tagagaagct gatgggcgga gatgcttcgg gcagggtttc cagataaaca
    20641 aaaataatat ggcaaaattt actaaacaac aggtaatcaa tacgatttac acaagcggta
    20701 tggtacctgt cttctacaac aaagacatag aagtgtccaa acaagtctta ctggcctgtt
    20761 accggggagg tgttcgagtt tttgaattta ccaaccgggg cgactttgca caggaagtct
    20821 tcaaagaact ggtcaaatac gccgaaaaaa catgtccgga aatgattatg ggtgccggct
    20881 cggtcgttga cgcaggtacg gcttctctgt atattcaact gggagccaat tttattgtag
    20941 gccccctgtt caaccccgaa gtggcaaagg tttgcaaccg caggctgatt ccttacgctc
    21001 cgggttgtgg ttctgtcacc gaaattggtt acgctcagga agccggttgc gatatatgca
    21061 aaatattccc agccggcaat gtgggtggcc cttcctttgt aaagaacgta atggctccta
    21121 tgccttggtc caagctcatg gttaccggag gtgtaacacc tgaagaaagc aacctaaaag
    21181 cttggtttga tgcgggcgta acctgcgtcg gcatgggttc taacttattt cctgccgacc
    21241 tgataaaatc cgaaaattgg gatggcatta ccaagctttg tgcagaagca atggctatta
    21301 ttcaaaaagt aaaaaagtaa tttatttaca tatttttcac acacaaatta catgaacatg
    21361 agtaaagcac agactgtgac gcaaaaaatg tcacaacaca gatgggtcat tgtggcattg
    21421 ctattttttg ccacaactat caattattta gacaggcaag tattatcact tacctgggat
    21481 gaatttatca aaccggaatt tcactgggat gaatctcact atggaacgat tacttcgttc
    21541 ttttctattt tttacgcaat ctccatgctt tttgccggac gctttgttga atggatggga
    21601 actaaaaaag gttatctttg gtccattggg gtttggagtg taggggcttg tttacacgcc
    21661 gtttgtggtc tcataaccga agcacatgta ggtttacaca gtgcagcaga actggctgcc
    21721 gctacgggtg atctggccgt tgtgatcgcc accttcagca tgtacagctt cctggttgca
    21781 cgaggtattc tcgctctcgg ggaagcaggt aacttcccgg cagccatcaa ggctacggca
    21841 gaatactttc caaaaaaaga ccgtgcttac gcaacctcta ttttcaacgc tggtgcatcc
    21901 ataggagcat tgtttgctcc cttgaccatc ccgccgctgg ccaaagcatt cggatgggag
    21961 atggcgttta tcatcatcgg agcattgggt tttatctgga tgggtttttg ggtttttctc
    22021 tacgacgctc caagaaaaag taagtttgtc aactcacagg aacttgaata cattgaacaa
    22081 gacaagcatg aagaaatagc cgaacaaaag aaaaaagcgg cggaagaaga aaaagttcct
    22141 ttcttgaaaa tactctcctt taaacaaacc tgggcttttg cactcggaaa atttatgact
    22201 gacggagtct ggtggttctt cttgttctgg accccttctt acctctacac ccaatttgat
    22261 atcaagatgt cggagggact cggtatggcc ttgattttta ctttgtacgc cattgtaacg
    22321 gtactttcca tcttaggtgg aaaactaccc acggtcttta ttaatcgagg cggacaaaat
    22381 ccttatgcag cacgaatgaa agccatgttg atttttgctt tcttcccgtt ggttgtatta
    22441 cttgcacaac ccctcggtat gcagttcgcc gagttgggtc aacatgctgc ctggattccc
    22501 gtattgttga tttcgatagg atgttctgca caccaggcct ggagtgccaa cctcttttca
    22561 accatcggcg atatgttccc ccatggtgct attgcaaccg taaccggaat tgggggcatg
    22621 gccggaggtg ttggctcaat gattttgcaa aaaggggccg gtaacttatt cgtgtatgcc
    22681 ggagaaacca acatgacgtt ctttggattt gtagggaaac ccgccggata ctttgtaata
    22741 ttctgtattt gtgccgtggc ttatctgatt ggctggatta ttatgaagtc cctggtgccg
    22801 agataccaaa agattgttat ttaattcagc tttctgccat tgaatgaact acgataaaag
    22861 agcatctcag aaaatgacat gaaccccgaa agttggtcgg tttaacatta ttaagaggtg
    22921 gtcttggata ggaataagag gccggctaaa aattgctttt tagtcggcct cttgcttttc
    22981 cccgatgatt gcctgagttt gtcagcgaaa acggaatgaa cagataaaat cggaaactca
    23041 ggaacactcc tactcaaggc aagagttgaa atttatttca acacataagg aggatcgggc
    23101 tctgcaatcg ctgcttcaat tttgtgtggg aatcccatct ctatagcctt tcccaggcct
    23161 ttgccctgca cattgtaaat acttaatgca tcgccctctc tgatattcac attaatattc
    23221 ctcaaaactg ccttccttac attggccaaa cggatacccc ataaagcttc tccgctgata
    23281 ttttccaaca ccaggccttc gagaggtttg tcggggtgca ccgaagtccc gtctaccaaa
    23341 tccttaacgt ctctgacacg aacgtttttg aaagaaaaat tctttacggt gggaatccct
    23401 tgcaatccgg gcaccggcac ctgatcctgc aaaccgctgc ccattaaatt gaaacggaga
    23461 aaaccgcctt gcatacccga tacgtcgaga ccttcggcac tgatatcttc gatgaaggct
    23521 cccctaccag gcctggattt aatgtaaatg gcatgggtac gggtgttctc aaaacggcaa
    23581 ttttcaattc tgactccccg aatacctccg gaggtttcac ttccaatacc gatgcaagca
    23641 taaatactgt ctgaaagcct gcaattccgt atgtacacat cttcggtggt acgcatcaac
    23701 atgttggctt ccatccccct gcccgacttg atagcaatac aatcatcgcc cgtttcgatg
    23761 tcacatcttt cgatcaggac atgtttacag gagtcaatgt ctataccgtc tccgttgccc
    23821 cctgtagatc tgatagtaag gtctttgaaa tgaatatttt cgcaataagt gggatgtatg
    23881 caccacattc tctggtaaga agtggagaaa tcgctaaaca gaagattttt acaattaatg
    23941 ggctctatca gggccggatg cctcagagga ttgtcactgg ttggcctgcc tcccaatgcg
    24001 ggatggccaa tgattttacc tccacccacg ataccggtat agctggcatc cactgcatat
    24061 atcagagccg tacgaccttg tatccacttg ccttcccaac gcacctgcat cacggggtaa
    24121 tcctcaaaat ggggcgtgcc cctaagaatg gcctccgaat gcaaacgcag caaaactccc
    24181 gaacgtaatt gaatagcgcc ggtcaaatag tcacctgcgg gaacaaacac ctctccgcca
    24241 cccaatacaa agcaacgatc aatggcgtgc tgtaaagcct cggtatttat tgtaattccg
    24301 tcgcccacaa caccaaaatc caacacattt aaatttaatt tgcccggatg ctgaggatat
    24361 tcaaaacccc tgatccggtt gtttgttttt gactcaattc gaggagcagg agctgccatc
    24421 aacgaattca ctgaacctac agagccgagc aaaggaatgg ccaaaaggcc cattcctgtt
    24481 tttttaataa aatcccgacg ggataatgga tcattcatgg taaataaaaa aatcagaaat
    24541 aaacaaactg ccgctaatgt aaggaatccg gccgaaaatc cggctttgac cttcaacaaa
    24601 aaagctccgt acaaaaaatg aactttgaat atactttatt gaactaattt tacacgctgt
    24661 ctattggcaa aatttgatta atttgtatgt ttatgataaa acgaacctga tcccgtatgt
    24721 atccgtgata atgccaaggc tgaacaaaca cttgctgact gtcgttcttt tcctgccctt
    24781 tctactaatg gctcaggaac agttgaccaa tctgcctaca ttgtacatta ccacagattc
    24841 gacttacgcg aacggaacta aagtacccat acaaaaagag gtttattttt caggctctct
    24901 tcgtgttgtt gccggtagcg aaactcccgg acaatacgac agcaccatac agatcagagg
    24961 acgaggcaat tccacctgga atctggctaa aaagccctat cgcatcaagt tggacaagaa
    25021 aacaaggttg ttgggtatga ctgccaaaga aaacgactgg gtgcttttgg ctaaccacgc
    25081 cgataaaacc ctggtgcgca atgctcttgc cttcgaaatc agtaaagcct gcaatattta
    25141 ttacacaccc gattatcgtt ttgttgacgt aattcttaat ggaaattaca taggtaatta
    25201 tatgcttacc gatcaggtag aacgcgataa agaccgtgtg aatactgaaa aaatggatac
    25261 ttcacgcctt gaccccaacc tgacaggagg ctatgtgctc gaaatagacg gttttggaat
    25321 taccggctct tacgatccga gtaaaggtga gttgtttccc gaagctttct ccagtaacat
    25381 aggcaacaaa gtaaccataa aatacccgga caacgacgaa attactgctg ctcaacgtca
    25441 atacatcatc caacattaca atgctttcga acaaagcatt ctaaactaca acaacaccca
    25501 ggttgcctac gaaaatattt gccgttatct ggacaccacc gcctttgtca attggtacat
    25561 cgccaacgaa ctgacagcca accctgatgg agtctggagt atttttatga agaaacaccg
    25621 ggacgacgac cgtctttatt ttggcccgct ttgggacaac gacatctcct ttggaaactg
    25681 caaccggata tattcgcttg caaacgacgg ccgctccgaa tccattatgt tgaaatgttt
    25741 cagtaacaac cgggtaaagg agcttataaa caaagtgctg accatttctc ccatcagaaa
    25801 aatgatcgct gaacgttgga aagttattta tcagaatgag atgctcaaag atgacctgat
    25861 acacctgctc tacaatatgg aagaagaact ggatgcttcg caaaagctga attaccaaaa
    25921 atggagcact gccctttctt cgaggattta caatgaaatc tattacggat ccaacattag
    25981 cttcgccatg cagatggaat atctgcgtaa ttatctcagc gagcgaattg atttcctcga
    26041 cagttttttt agcgccatgg ccaacgaagg cggcattgat cccgaagatc ctcctcttcc
    26101 tccggtggat acggcgacat atttcagccc cgagccttat gtatggtacc atctacaatg
    26161 ccaactcaac gataaatatt tggtttgtca agaggtagcc gggggatatc agcttgatta
    26221 tacttacgat ccggaaaccg cgggcgattg ggcaaaattt gaaatcagtt gcagcaaaga
    26281 cgatgaaaac cgcagtgttt actatctccg caataaggct gccaaaacct atgtctatat
    26341 caatgctgct aaggcagcaa ttcctctgga tccggtccaa aaaacgtttt ttacgcttta
    26401 tcccagccca aacgaagccg ggcattacgg acttatggtt ctttcggaca acagcactct
    26461 ccatgaattg ggcatcgatg ccaatggtag cgtggtctct tggcctacct tgaacaaaaa
    26521 gggtcaacgg gaataccggt ttattcccga ccgctctcat ttccaaagcg tcttcgaccc
    26581 acccgatccc caacaggcaa tgtgggacat ggaagaaaat tgggacaaca acattcccaa
    26641 tgcctacgcc acagccatta ttgcagaagg ggcaaaactg gaattatccc aagacgaaac
    26701 cgtaacggta gacagtctga ttattttgcc cggtgctgaa gtcttgaatt acggtaccat
    26761 ccgggccgat gtcagcctgt tttgctccaa cgacagcctg tcggcctctt acgattacga
    26821 tctggaagct caactgatat ccgaacgcgt gctttaccgg agttttaatc gcagccaaag
    26881 ttggtatgcc ctatccttgc cactcgacgt aaaatcggta cgcaacaaag aaatgaccat
    26941 attacaaccc ggcaaagatt attacctgat ggaatacaag gccgactcca accgttttga
    27001 ttcgctgagc ctggcaagca gcttcaaacc caaagcaggc ccttatctgt tctcggtcag
    27061 cccggaaaga gaacagcaag cttttatttt tgtattcgat accaaaagtt cctatcaaac
    27121 cggcaacagc gattacaact ttaccggcaa caccggtttc aaaacaataa acatccctgc
    27181 ggcaggtacc tacctactgg aagatcattt gcattttgta ctgcaggaag atcagcaaca
    27241 attgtcgctt ccggcttttc atgcctactt ttcagtcagc ggagacaggc tgcccgattc
    27301 tctggccatt tacaacatga atccagccaa cgacaccctg gccttggaga acatatccaa
    27361 acaggggccc ttactagtga gcagagcagg tttgttgatc attttcaaca tggaggacgg
    27421 agaagatttt tccctgttca acctggacgg caagctgctc ttcaaagcca aggctctgaa
    27481 caatcccact tatgttcctc tgcaacaagg tatttacctg cttcgataca aaaagcgttt
    27541 ccataaaata catattccat aataacccat aaaaatacaa gctatgaaaa aaagaatcat
    27601 tagtacattt atactcacag ccatcgttct gagcgtttcg gcgcaaaaaa acgagtggca
    27661 ggatccgggt ataaacgccg tgaacagagc tgaaatgcac accaattatt ttgcctacga
    27721 atcgcgggag gcagctctga aaaactgtag agagagctcc agtaattatc tcagccttaa
    27781 tggtatgtgg aaattctact gggtcaaaga cgctaataat cgccctacgg actttttccg
    27841 taacgatttc gacgaccgaa actgggccaa tatgccggtg cccggtatct gggaaatgaa
    27901 cggctatgga gatccggtct atgtaaacac aggctgggcc tggcgcaatc aattcagaag
    27961 caatcctccc gacattcctg tcgaaaacaa tcatgttggc tcctatcgcc gggaaatcac
    28021 tattcccgat cattggaaag gcaaagagat ttttgcccat ttcggttccg taacctccaa
    28081 catctacctt tgggtaaacg gtaaatacgt aggttacagc gaagacagca aactcgaagc
    28141 cgaatttaac ctgagtaagt atttgcgtcc cggcaaaaac ctgattgctt tccaggtatt
    28201 tcgctggtgc gacggcacct atctcgaaga tcaggatttc tttcgcctga gcggcgtggg
    28261 ccgtgattgc tatctctacg cccgcccttc gaattatatc caagacatca ggatcactcc
    28321 cgacctggat gcccaataca aagacggcag cctgaaaatt gacgtcagca tgaagggcaa
    28381 aggagaactg gaattgaatc tgctggatga aaacaatgaa accgtagcca gtacacaacg
    28441 aaatgcaagc ggcaaattca gtctgatgat gaatctgagc aatccccaaa aatggtcggc
    28501 cgaatgcccc aatctctata ccttggtggc cactcttaag gggggcggcc aggtgctgga
    28561 agctattcct gtcaaagtcg gtttccgtaa aattgaactc aaaggcgatc agctactggt
    28621 caatggacag ccggtactgt tcaagggtgt caaccgacac gaaatggatc ccgactacgg
    28681 ttattatgtt tctccggaac gaatgatcca ggacctcaga ctaatgaaag aacacaacgt
    28741 gaatgccgta cgcacctgtc actatcccga caacaatctg tggtacgatc tctgcgatga
    28801 atacggtctt tatgtggtgg cagaagccaa catcgaaagt cacggtcttg gctacggcga
    28861 aaggacaaca gccaagcgtc ccgattacgc tctggcccac atggagcgca atcagcgcaa
    28921 cgtacagaga agcttcaacc atccttcggt cattttttgg tctttgggca acgaagcagg
    28981 ttttgggccg aattttgaaa tgtgttacaa gtggatcaaa gccgaagacc cctcccgtcc
    29041 ggtacaatac gaacaggcca ggaccaacga atttacggat attttctgcc ccatgtatta
    29101 cggctacaac aattgcatac gttattgcga aggcgatatt caaaaacccc tgattcaatg
    29161 cgagtacgtg cacgccatgg gtaattctga gggaggcatc aaggaatact gggacctggt
    29221 gcgtaaatat cccaaatacc agggcggttt catctgggat ttcgtagatc agtctctgcg
    29281 gaagttcgac aaaaacggcg ttatgtttta cgcctatgga ggagatttca acctttatga
    29341 tgccagcgac ggcaatttct gcgacaatgg cctgatcagc cccgatcgcc tacccaatcc
    29401 tcatttccat gaagttggcc atgtatacca gtccatttgg gccagtgcct ccgatttgga
    29461 aaaaggagaa atcgaaattt acaacgaata cttcttccgt gatctgagtg ccttttacct
    29521 tgaatgggcc ttgctggtaa acggtaaagt ctgccaaagc ggtatggttg acaagattga
    29581 tatcgaagcc caacaaacca aaaagctcaa actcgattac gacttaagcg gcatcagccc
    29641 cgacaaggaa gtattgctga atatttcttt caaactaaga aaagcagaac aattattgcc
    29701 cgccggtttt gtgttggcca aaaatcaact ttgcatacgc gatcgctcaa agcaaagctt
    29761 gaattttgat aatatattga ctgccaacat cgagctacag actcctaaag taatcgaaaa
    29821 cgactggaga ttccttatca tcgaagccga taattaccgt atagaattca ataagcacaa
    29881 cggttacctg agccgctggc aagtaaaggg gactgatctt ctgaacgaag gcggcagcct
    29941 cacgcccaat ttctggcgcg ctcccaccga caacgatttc ggagccaatc tgcaaaacaa
    30001 gttggccgtc tggaaaaatc cgggtttaag actggaatcg tttgaacaag ccatcgaaga
    30061 tggtttggtg gtagtaagag ctcagtacga tatgcgtgct gtttcgtcaa aactccatct
    30121 aacttaccgc atcaataatc agggcagcat agaagtcact caaaaaatga ccgccggcgc
    30181 cgatgcaaag gctcccgaat tgttccgttt cggcatgcaa ctgcaaatgc ctttgctaat
    30241 ggacagaatt gagtactacg gccgtggtcc gatcgagaat tattcggaca ggaacaattc
    30301 taccgatttg ggtatttaca aacaaacggt tgaggaacag ttttatccct atatcagacc
    30361 tcaggaaaac ggcaataaaa ccgatatccg ctggtggaaa caaacgaatg ccggaggcag
    30421 aggtataaaa atccgctcgg tcgctccttt ctcggcctcg gcactgaatt attccatcga
    30481 atcgctcgac gacggtccca gtaaaggaca aagacactcg cagcaaatac ccaaagtgga
    30541 ttataccaac ctttgcatcg acaaagtaca aatgggtctg ggtagtgtaa acagttgggg
    30601 agctatgccc agagacgagt atcgcctgcc ccatcaggac tacgaattcc aatttgtaat
    30661 ggaagtccgt taaaaaatta gtgtaaatgc tgaagaggat gacttttagt catcctcttt
    30721 ttttctgttc attcagtatc cgtctgcaaa gtcaggcaat catcgggaag attatgccat
    30781 caacaggcaa agtaattcac gaagcaattc cgactcaggc aaggccgcct gctcaaaccc
    30841 cttgagcctg gcgtcgtaat gccttatgag acttatgttt cgcacaactt tattcgcatt
    30901 gtaatgttga gctccctgct gatagttttt tactaaaaat ggaggaattc ccagttgtga
    30961 agcaacattg gaagaggatt tgtcgggcag gtaatggtac atcagcagtt ttgaaaaata
    31021 atcaaacaaa acaatcagtg taaccaccat ggcattgttt ttgggattcc gggcgaaata
    31081 ttgcgcaatt tgttgggctt tgaattggtc tttttgcttg atggcatcga taagttcaaa
    31141 attgttgtac tccttgctga cgccgatatt cagctcaacc atggccgccg taatcttata
    31201 aggcttgatt ttgagcaata atttcagttt ttccagttcc cccacaatgc gccccaaatc
    31261 ggtacccaaa aaatcggcca gcatttgggc agctttggct tcgatctgta tctcctgctc
    31321 cgataaaaag cgggtaatcc aggcgggtat ctggttgtcg tacatttttt ttgattcaaa
    31381 taaaagggcc gattgctcta gggctgaata gagttttctt cttttgtcga ggcttttgta
    31441 tttatagtta ataatcaaaa tggtcgaagg catcggttgt tgaacataaa gtaaaaactc
    31501 ttcttcgaaa ttgcgtatca tctgggcttc gcgcaccaat atcacctggt aaggggctaa
    31561 cataggataa cggcgggctt cgttaataag ctgagcaaca ctgagatctt tgccgtaaag
    31621 tatgcgctgg tcgaaatctt gctgactttc ggtcaaaaca agtttgagca gacgattgct
    31681 tatctcgtca atgtaatagg gctcctctcc catcaaaaag tagattggat accgcttgcc
    31741 ggcttctatt tcccgaatga cttcctgtgc attctccgtt tttttggcca tataaatcct
    31801 gatttcacaa gtattgccgt atctttgaat acaaattgat tccaaaaata cgaaatgttt
    31861 gcattaaacc tgcccggctt caagtacaaa ttgcgcaaga aagatgctaa gaactacata
    31921 ttcgaccccc tcaggcgtaa atacgttttt ctcagtcccg aagaatgggt gcggcagcat
    31981 ttttgcaatt acctgattgt ccataaaaat tatccgtccg gacttatcgc caacgagtgc
    32041 agcatccaac tggaaggctt aaccaaacgt tgcgacaccg ttatatacga tgctcatatg
    32101 caagtattta tgctggtaga atacaaagct cccacggtaa cgctcaatca ggaagttttt
    32161 gaccaaattg tacgttacaa ctgggcttta caagccaaat acctgacagt cagtaacggt
    32221 atgaaccatt attgctgcag gatagattac cttgacaata cagtgaattt tttggaagat
    32281 atccccgctt acaacgaggg ttgattcctg caaaaaccgg ataaaaatta tttcttaccg
    32341 gccggttctg ccggtgtttc cactcctgcc aattcgggat cgatcataat gcggccacag
    32401 tattcgcaaa caattacttt tttgcgcatt ctgatatcca tctgtctttg gggcggaatt
    32461 ttattgtagc aaccaccaca ggcatcgcgc tgtacgtaaa ctacggctaa accgtttctt
    32521 gcgtttttgc gtatacgttt gaaggcttgc agaagcctgg gctctatgta actttcgatg
    32581 gttttggctt tttccctgag tgtctcttct tcagagcggg tttctgaaat gatttcatcc
    32641 agttcggatt tcttcacttc caagtcctcg cgtctttcgt tcaattcttc ctgagccaga
    32701 ttcaattctt cgtttttagc tttttcgctg aattgagctt cccggatttt cttctcggca
    32761 tgttcgattt ccaaaccctg aaattcaatt tccttgctca gaaaatcata ttcccggttg
    32821 ttacggacat tgtcttgctg ggctttgtat tttgcgatca gagccgtaga attttcaata
    32881 tcgatcttac agcccaaaat tctgtcggta catgttttta tctctgcctt gaaattttcg
    32941 atgcgggtct ccaggccggc aatgatgtct tccaaatcct gcacttcgag cggaagctcg
    33001 ccgcgaagaa ttttgatttt atcaatttcg gaagctacaa cctgtaattc gtacagtgct
    33061 ttaagctttt cttctacaga aatttctttg ggttcagcct tagtactatc gacgctcata
    33121 tttatataaa attttgtgat ttagctgttt aagtataaaa aaccggattc gtctctcttt
    33181 gggacgaatg gacggcaaag ttagcaatat tttttgaaat taactctaca aacaggtctt
    33241 ttgtaaattg ctccgtttcg taatgtccgg catctataag caacaattta tcgtctactt
    33301 cggcaaaccg gtggtatttg acgtcggcag ttataaaggc gtctgcccct tcggcgatgg
    33361 ccgccgaaat aagttcgctg cctgagcctc cgcaaacagc cactttgttt atttcccgac
    33421 ctgtaagagc ggaatgtctc aacattttgc ttccgaatac ctgttttagg agtattaaaa
    33481 aatccttttc ctccagcgcc cgagtcagaa atccggtcat accggcaccg acattgctca
    33541 tgttttcttt agggactaaa atggcagtag agcttaaatt caatttctcg caaatttttc
    33601 cgttcacacc gtccataaca gcatctacat tggtgtgccc ggcataaatg gcgatcttgt
    33661 tttgtatggc tttaatcaag gtacgctctt gcggattttt ccccgtgatg cttttgattc
    33721 ccctgaaaat aagaggatgg tgggaaacaa ccatattaca tcccttttca agagcttcgt
    33781 ccaataccgc ctcggacaca tccaaacaaa ccaatatcgc tttgacctga gtagcaggat
    33841 ctcccagtat caaaccagcg ttgtcgtagt cctcctgaag ttttagaggg gcaaaattct
    33901 caattaaagt acatatgtcc cgaactttca ttctttgttt tttggagtaa gtatttttaa
    33961 gtgcaattca tccagttgtt cctgatcgat gtttgaggga gcatctatca ttacgtccct
    34021 gcccgaattg ttttttggaa aggcaatgca atctctgatg gaatccaaac cggcaaacat
    34081 agacacgcaa cggtcaaagc caaaagcaat accgccatgg ggaggtgctc cgaatttgaa
    34141 ggcatccatc aggaaaccaa aacgctgagt agcctcttct ttagtaaagc ccaagatctt
    34201 aaaaacatcg gcctgcaacc ggctatcgtg tatacgaata gatcctcctc ctaattctac
    34261 tccattgatt accatatcgt aggcatttgc cctcacctgt gagggatctt tttccaacag
    34321 aggtacatct tcgggcttgg gagaggtgaa aggatggtgc atggcgtaat aacgggctgt
    34381 ttcttcgtcc cattcgaaca tggggaaatc gaccacccac aaaggaacat atagattttt
    34441 gtcgcaaaat cccagtcgcc ggcccatttc gaggcgtagt tcgctgagag cttttcgagt
    34501 gacttgtgtg gctcccgaca aaatcagtat taaatctccg ggctgagctt ggcaagcttc
    34561 ggcccaggtt ttcagatcgt cctgagtgta aaatttatcc accgaagatt tgaagctgcc
    34621 gtctgcttcg caacgaacat agaccatgcc tttggctcca atctgtggct ttttgacgta
    34681 ctctgtcagt tcatccaact gcttgcgagt ataagaggcc gatgcgggtg cacaaatagc
    34741 tccgacatat tcggcctggt caaaaacatt gaaagaacgc cccgtcgtaa ggtgcttaat
    34801 ttcaacaaaa gacataccaa agcgcagatc aggtttatca ctgccgtaat aacgcatggc
    34861 atcggcccag ctcattcgcc taaaagcctc tttaaattca atgcctttga tttctttgaa
    34921 caaataacga atcaaaccct caaaggtatt aagtatgtct tcttgttcaa tgaacgacat
    34981 ttcacaatcg atctgggtaa attcgggttg gcggtcggct ctcaggtctt cgtccctgaa
    35041 acatttaacc aactgaaagt agcggtcata acccgaaacc atcaacaatt gtttaaacat
    35101 ttgaggcgac tggggcaaag cataaaactg acccggattc attctggagg gtaccacgaa
    35161 atctctggct ccctcgggag tggatttaat cagtaccggg gtctctattt cgaggaatcc
    35221 ctgcttatcc agatagttgc gggtggccat agccatttta tgtctcagtt gcaaattcct
    35281 gctcaagggc ggacgcctca gatccaaata gcggtaggtc attctaagct cgtctccccc
    35341 gtcggattgc tcttcgatgg taaaaggcgg cgttgcggca gcgtttaaaa ccttgatttc
    35401 ttcgacctga atttcaatat caccggtgac caattgaggg ttctttccgg atcgttccct
    35461 gaccagacca tgtacctgaa tcacccattc ccggcccatc ttattcaccg tttcaatgca
    35521 agcggcaccg gcttcctggt cgatcaccaa ttgagtaaga ccgtaacggt cgcgaagatc
    35581 aacaaaagtc acagctccca ttttgcgtat acgctgtacc cagccggcca aacaaacttt
    35641 tttgcttaca tcatgaatac ggagttctcc gcaagtatgt gttctgaaca taaacttctt
    35701 tgtttaagac tgcaaaggta cattttccga tgatattttt aatctcttgc ttgtaaattt
    35761 tattctttaa cctgcagggt cttttgagat gtacaaaatt tgtgcttcct ttgcattcag
    35821 aaggaacaga ccacttatat ctttttataa tcctatgaat caagatctta ataaagtgct
    35881 ggtgctgggg tcaggcgctt taaaaatcgg acaggcaggc gaattcgact actcaggctc
    35941 gcaagccctc aaagctttgc gtgaagaagg catccgttcc gtgttggtaa atcccaatat
    36001 tgcaaccatt caaacctctg aaggaattgc agatactgtt tatttcttgc ccgttactcc
    36061 ttattttgta gaaaagatca ttgaaaaaga acgccccgac gggatcttgt tggcttttgg
    36121 agggcagaca gccctgaatt gcggtaccga actttacctt tcgggagtgc ttaaacaata
    36181 cggagtcaaa gtactcggca cttcggtcga agccatcatg tataccgaag accgcgatct
    36241 gttcgttaaa aaactgaacg aaataaactt aaaaactccg gtaagccagg ctgtggaaac
    36301 tatggaggca gccctgaagg cagccgacaa aatcggctat ccggttatga ttcgttcggc
    36361 ttatgccctc ggaggacaag gtagcggcat ttgcacgaat cgggaagagt tcatcgcatt
    36421 ggcccaaaac gccttctcct tttcaaaaca gattttaatt gaagagtcgc tcaaaggttg
    36481 gaaagaaatc gaatttgagg ttattcgcga caaaaacaac cactgtttta ccgtagccaa
    36541 tatggaaaac gtcgaccctc ttggcatcca tacgggggaa tcaatcgtgg tggctcccac
    36601 ctgctcgctg gacgacacag agctcaatat gctgatagaa ctctccaaaa aatgcgtccg
    36661 gcatttgggt atcgtaggcg aatgcaatat tcagtatgcc ttcaactcgg aaaccgacga
    36721 ttatcgcatt atcgaagtga acgcccgctt gagccgttct tcggcactgg cttccaaagc
    36781 caccggttat cctctggctt tcgtggccgc caagctggct ttgggttata cactggacca
    36841 gataggcgaa atgggcacca gtaattcagc ttatatagcc ccccaactcg attacatcat
    36901 ttgcaaaata ccccgctggg atttaaccaa atttgccggc gtttcgcgcg aaataggttc
    36961 ttctatgaaa tctgtcggcg aaatcatgtc cataggaaaa tcctttgagg aaaccattca
    37021 aaaaggactt cgaatgatcg gccagggcat gcacggtttt gtgggcaacg aaggtgccga
    37081 attcgacaat cttgaccagg agctgtcgcg ccctaccgat ttgcgtattt ttgccatagc
    37141 cgatgccctg aaaaaagggt atagcgtgga acgtatctgt gagttaacca aaattgatcc
    37201 gtggtttgtc ggtaagctca aaaatatata cgattacact ctggttttaa aacaatacga
    37261 aaaaattgag gagttacccg aggatatctt actggaggct aaacgcctcg gtttctcgga
    37321 ttttcagatt gcccgttttg tcgaaaatcc taagggtaat atggaaaaag acaatatccg
    37381 cgtacgagat cgtcgtaaac aactcggtgt actgcccaag gtgaaacgca taaacaccgt
    37441 cgcttcggaa catcccgaat tgaccaatta cctttatctg acttacgaag gtgaaactca
    37501 tgatattccc tattatccca acgagaaatc ggttgtagtg cttggttccg gagcttaccg
    37561 catcggcagt tccgtagaat tcgactggtg ttcggtcaac gctatccaaa cagcccgcaa
    37621 cttgggttac aagtccatca tgatcaatta caaccccgaa acggtatcga ccgattacga
    37681 catgtgcgac cgtttgtatt ttgatgaact gagttttgag cgcgtgatgg acgttatcga
    37741 cttggaacgt cccaaaggtg tgatcgtttc tgttggtggc caaataccta acaacctggc
    37801 catgaagctt taccgtcagc aagttccgat cctgggcact tctccggttt ccatcgaccg
    37861 ggccgaaaac agacacaagt tttcgagtat gcttgatcag ttgggcatag atcaaccagc
    37921 ctggaaagag ctaaccagct tagaggacat caagatattt attgaaaaag tcggttatcc
    37981 tgtgttggta aggccttcgt atgtcttatc gggtgcggct atgaacgtct gttacgacga
    38041 agatgatttg gaaagtttcc tgaaaatggc ggctaaagtg tctaaggaat ttccggtggt
    38101 ggtttcacag tttttacaaa acgccaaaga aattgaattt gacgctgtgg ctcacaacgg
    38161 ggatgtggtt gaatacgcta tctccgaaca cgtggaattt gccggcgtac actcgggcga
    38221 tgccacgatg gttttcccgg cacaaaaaat ctattttgcc acagccagac gcatcaagaa
    38281 aatcagtcgc tgcatagcta aagaactcaa catcagcggc ccgtttaata ttcagtttct
    38341 ggccaaaaac aacgaggtca aggtaattga atgcaacctc agagcttcga ggagcttccc
    38401 ctttgtatct aaggtactta aacgtaattt catagatacg gctacccgca tcatgctgga
    38461 tgccccctac gacaaagccg ataaaaatgc ttttgatatc gactggatag gtgttaaagc
    38521 ttcccaattc tctttctccc gtttacataa agccgatccg gtactgggcg tagacatgtc
    38581 gtccaccggc gaggtaggct gtctgggcga cgatttcggc gaagccttgt tgaacgccat
    38641 gattgctacg ggttttaaca ttcctgataa gaaaaaaggc attatggttt cttccggagg
    38701 aagcaaatcg aaagttgact tattggatgc ctgcattatg ttgcaagaaa acggctatcc
    38761 catttatgcc accggaggaa ctcagcaatt tttaaaagaa cacggtgtaa aagccgaatt
    38821 ggtacactgg cccgacgaaa ataacgacaa gcgacaaata ttaaacatga tctctgatcg
    38881 cgcttttgat ttgcttatta atatacccaa aaatctgact aaaagagagt taactaacgg
    38941 atataaaatt cgcagggctg caatagatca caatataccg ttgattacca acgccaggtt
    39001 ggcagcagcc tttattgagt ccttctgctc catcgacatg aatgatatcc agattaaaag
    39061 ctggcaagaa tatatataat ttacctatca tcgcaatatg ggaggctttt ttggaaccat
    39121 ctccaactca agttgtatca aagaccttta ttacggcata gactacaact ctcacctagg
    39181 aacccgccgc ggaggcatgg ccactttata taacggcaag ttttataggg ccattcacaa
    39241 tttggaaaat gcttattttc gctcaaaatt cgagccggag ctactttctt ttttgggaaa
    39301 ctcaggcata ggtgtcatta gtgataccga tcctcagcct atcgtaatta attctcgtct
    39361 gggaaaattt gccatagtaa cagtggcctg tgtcaataac ataaaagaac tcgaaaaaga
    39421 tttactcgat tccggcaggc atttttccga actcagttca gggcaaacca atcagacaga
    39481 actgattgcc ctgatcatta cccaaggcaa gacttttgtc gaaggcattg aattggtttt
    39541 tcaaaaagta aaaggatctt gttccatgct gatccttacc gaggaaggaa tcatcgttgc
    39601 ccgggacaag ttcggacgca cccccattgt cattggcaaa aaagacggag cttatgccgc
    39661 ttcgagtgaa ccctgtgctt ttcccaacct cgattataaa acggactact gtcttggtgc
    39721 aggtgaaatt gttcttctcc gtccaaataa catgcagcaa ttgcgtgagc ccaacaaacg
    39781 caaacaagtc tgttcctttt tgtgggttta ttttgggtat cctgtttccg actatgaaaa
    39841 tataaacgtg gattcagtcc gttataaatg tggtttaacg atgggtaaca acgacgattc
    39901 agaagtagat ttcgtctccg gtattcccga ctcaggtgta ggtctaggtt tgggctacgc
    39961 cgaaggtaaa ggcgttccct accggcgcgc catagtaaaa tacaccccca cctggcctag
    40021 aagcttcact cccagcgacc cgggtatccg taaacttgtg gctaaaatga agctgatacc
    40081 caaccgtagt ctactcaccg gcaaaagggt tatcttttgc gacgactcca tagtacgtgg
    40141 cacacaactt tccgacaatg tgagcgaact gtacaagtat ggagccaaag aagtgcatat
    40201 acgcatcgga tgtccgccgc tcatttacac ctgccctttt ctgcatttta ccgcgaccaa
    40261 gtccgacttg gaactgatta cccgccggat aatacaggag atcgaagggg ctcacgacaa
    40321 aaacctgtct gaatactcca aaaccgattc tgtcaaatac cggcaactgg tggaacacat
    40381 ccgtgcccgg tttgacatta catccctaaa attcaactca ctcgaaaact tggtcaaagc
    40441 cattggttta cctaaagaaa gcttatgtac tcattgcttc gacggctcca gctatttcga
    40501 ttgagtttgt tctgaaataa ataaaaaaca ctatctttgt cgccggaaat attcggggtg
    40561 tagcgcagtc cggttagcgc acctgctttg ggagcagggg gtcacaagtt cgaatcttgt
    40621 taccccgaca agtgaatcac aacgccttta ttgcgatgag atttttcccg aaaaacgacc
    40681 cttttactcc aactaatgag agagggtcgt ttcttatttt ttctccgatt gaaaacaaca
    40741 aaaataattc ctgattaatt agggaaaaaa ttctgtaaat agaatattat tgtatatctt
    40801 tacagtgttt ttacaaaaca cagcaaaatc cttttttatt aaagcttttt gctgttattt
    40861 attttcgttc tacattaacc ttaattttca tgacacacat caagtcgctt gagcaactaa
    40921 caagcaaaag ggatgaactc aaatcgaact tatcccggct ggatgacccc aacaattccg
    40981 agaaaaaagt acagatcaag gttgccatgg caacctgcgg tattgcttcg ggcgcgcgta
    41041 ccactatgga ttttttgact gccgaactca gtaagcgaaa tattccctct gtggttactc
    41101 aaaccggctg catgggctat tgctacgcag aacccaccat tgaagtgaaa ttacccggca
    41161 aagaagccgt aatctttggg tatgtggaca ccaagagagc cgatgaaatt attgaaaaat
    41221 atattaaaac gggagaacta acagagggag ttattccccg gaattacgac acaatcgatt
    41281 aatcatagta cgatggaaac acctgtcaat aaacccatgc agctgcgtat tgccctgcgt
    41341 aactgtggca tgctcgatcc ggagaacata gcggaatcta ttgtccggga tgcctatcag
    41401 gccctgggta aagtgttaaa agaatacagc ccccaggaat gcataaacat tttaaaacaa
    41461 tccggccttc gaggacgcgg aggggccggt ttccccaccg ggctcaaatg ggaaattgcc
    41521 tccaaaagta aatccgatca aaaatacgtc gtttgcaatg ccgacgaagg cgacccggga
    41581 gcctttatgg atcgatccat actcgagggc gatccgcatt cggtgctcga agctatggcc
    41641 atttgcggtt attgcatagg agccgacaaa ggactcattt acatacgagc agaatatcca
    41701 ttggctatta aacgattaaa aatagctatc agtcaagcga tggaatgggg cttgttgggc
    41761 aaaaacatcc tgggtagcgg ttttaatttc gaaattaagc tctgctacgg tgccggagcc
    41821 tttgtttgtg gtgaagaaac cgccctgatt cattccatgg aaggcaagcg cggggaaccc
    41881 accaataaac ctcctttccc tgccaccagc ggttttatga aaaaacccac caatgtaaat
    41941 aatgtggaaa catttgccaa catagctccc atcatactca agggcccgga atggttttcg
    42001 tccatcggga ccaaaaactc caaaggcacc aaagtgtttg ccctggcggg taaaatcaat
    42061 catgtcggac ttatcgaagt ccctatgggt acaagcctga gagaaatcat ctatgagatt
    42121 ggcggaggaa tcaaagaaaa caaaaaattt aaggcggttc aaaccggagg accttctgga
    42181 ggttgtctga ctgagaaaca cctcgatacg cccatagact acgaaaatct gattgccgcc
    42241 ggttctatga tgggttccgg aggaatgatc gttatggacg aaaacgactg tatggtttcg
    42301 gtagctaaat tttatctcga ttttatagta ggagaatcct gcggtaaatg tacaccttgc
    42361 cgggtcggca ataaacgctt gtatgaaatt ttggaaagaa tcacccaggg caaaggggta
    42421 atgaaagacc tggataaact gaaaaatctc agctatataa ttaaagacac tgctctttgc
    42481 ggtttgggac aaacctctcc caacccggtg ttctccactc tggagaactt ttacgacgaa
    42541 tactacgaac atgtggtcga gaaaaaatgt cgtgccggcc attgtaaaaa tctgttccac
    42601 tacgttatca acgaagaatt gtgcgtgggt tgtgctgcct gcaaaaggtg ttgtccggta
    42661 cacgccatca ccggcgaaaa acgcaaacct cattacatcg attccaatat ttgcatccgc
    42721 tgtaatgaat gcatcgaaaa atgctctttc gatgccatta gtattcaact cgaacctaaa
    42781 atcttagtat aatggagatg atcgaattaa gcattgacaa caaagtgttg agagtaaaaa
    42841 aaggtacaac cattctcaaa gctgcccgca aagtgggaat cgaaatcccc actctttgtc
    42901 atatggagct tcacaacatg aatatagaaa acaaaccggg cagttgccgg gtatgtgtgg
    42961 tcgaagtaga cggacgtcgt aacctggccc ccgcctgcat caccgaagtg acacccggca
    43021 tggtagtcaa tacccataca gtcagggtgc tgaatgcccg taaaaccata gtggaattat
    43081 taatttcaga ccacccctac gattgtctgg tttgcgccaa atcgggaaat tgcgatttgc
    43141 agtcgatcgc tcataaattc ggtatccgcc gcgtacactt taaaggcgaa aaatcgacct
    43201 accggaaaga tacttcgcct tctataatac gtgatgtcga caaatgcatt atgtgccggc
    43261 gttgcgaaat gatgtgcaac gaggtgcaaa cagtggg
//
LOCUS       DAOSTD010000004        41528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1061_length_41528_cov_13.990283, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000004 DAOSTD010000000
VERSION     DAOSTD010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 41528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41528
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1061_length_41528_cov_13.990283"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1590)
                     /locus_tag="PLI37_00495"
     CDS             complement(7..1590)
                     /locus_tag="PLI37_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOQ57527.1"
                     /translation="MKSFSHILPILLMLSLSSQVCSAPKDSKQPPRLVLGIHLDQLQE
                     EYLKWFMEGFGEEGFKLLLERGKVYNNCNYGLNCPDAANTNACLLSGCAPMHHGIIAR
                     EWYDRQLERPVSCVYDTDYLGNYTTSTFSPLSLRSNTLGDELKKACKGRSKVFSIGLE
                     AEAAIVAGGRSADAALWMDDASGKWCSSTYYNYMPSWLQKINDYGLFETNNGKNGWRP
                     KFPLSRYVYMPHQKNPGFFSYALSFINGTGYRDFKQTPMANTELNQLALELIDQEKLG
                     EDEYPDLLILHFNTGSQLSGNDSRAALEMQDLYFRLDQDLGWLLKEIDRKIGLDNVLI
                     YLCGTGATRLAVEENPKAPGFYGNYYPERTTALLNLYLTAIYGSERWVQGCTENEIYL
                     NRKRINELNLDYREICNKAALFLTQVEGIQQVVPAYCFLLGDWSSESALSLNYHKERS
                     GDLLLKLENGRNVRWESYPGKNRQIQYANQTTILVFYGAGIKASNHSQLVSIFDLTPT
                     LCGMLYIRPPTASTGQVLF"
     gene            complement(1587..2834)
                     /locus_tag="PLI37_00500"
     CDS             complement(1587..2834)
                     /locus_tag="PLI37_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOQ57528.1"
                     /translation="MLFFVGISPGVKAQTVPALSGQAEIYLLTCSPGQEVWAHYGHTG
                     IRVLDPPTRRDVVFNYGIFDFNSDNFLWNFVRGEIDYILGISSFEGFMHSYRNENRRV
                     ISQKLNLSQEEKAKLWEALVINSLPENRTYRYNFYYDNCSTRARKIIERCLDGAVDYH
                     YEAAYGSMREVIQHYTAKHPWTWFGISLALSSLADAPAPSLDLLFAPELMMEAFEKAT
                     ITAPATNGKDSLQAERCLISEQSILNEIKTVPAEQKNTLPGPVTFSWILFTLVLTLGL
                     RELKTACRYPLVNALIFGLAGIAGLIIAFLALISVHPVTKTNYLLLWLHPLHLLFALL
                     IAFKPFRRSIFYNGYLKINTPVLVFALAGFGFLPQYVHPAMIPLLLSLLSRSLLAWIN
                     PLPKSIRNKKVPGQGSGKSGKNL"
     gene            3100..3483
                     /locus_tag="PLI37_00505"
     CDS             3100..3483
                     /locus_tag="PLI37_00505"
                     /inference="COORDINATES: protein motif:HMM:NF039701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HOQ57529.1"
                     /translation="MKIDYSRLGEWVQNNTLLMVFLLGFVAILLFVFLIVRYKNNKNR
                     LLLAQEYLAAGKEIPRYLLRENSLYARAVKNLFLGIGLVFFLGFLTETWGWASAGLLF
                     IALGVGQMIVFKKSSKDFTGSDLDR"
     gene            complement(3513..4373)
                     /locus_tag="PLI37_00510"
     CDS             complement(3513..4373)
                     /locus_tag="PLI37_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOQ57530.1"
                     /translation="MQTIRFCERLRFCALCLLFSLAGTLQAQEWKLVWSDEFNTEGPP
                     DSGSWNYERGFVRNEELQWYQPGNAYCRGGLLIIEGRKEKVPNPRYNPQARDWRTSRQ
                     QADYTSSSITTRNKRSFLYGRFEVRARIPLAGGSWPAIWTLGNTMEWPSCGEIDIMEY
                     YRIKGEPHILANAAWGGEQRYKAIWDSSVYPLNHFTNADPDWGSKFHIWRMDWDEQAI
                     KLYLDDELLNEIPLDRTSNGSIGNYSNPFRQEHYLLLNLAIGSNGGKPDESAFPLFYE
                     IDYVRVYQKQ"
     gene            4517..5701
                     /locus_tag="PLI37_00515"
     CDS             4517..5701
                     /locus_tag="PLI37_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOQ57531.1"
                     /translation="MSEKFKINRFVHYVGVNDRKTHLFENLWPLPLGVSYNAYLVVGE
                     KIALIDTVESCYASELLEMIVRTVGNRPLDYLIINHMEPDHSSSINAIRQAYPGVQIL
                     GNAKTIQMLEGYYGAHQGIIEVKEGDSLCLGGETLRFYLTPMVHWPETMMTYCEQEQL
                     LFSGDAFGTFGALNGAVLDQDMDLSPYWDEMYRYYANIVGKYGSPVQKALQKLSGLDI
                     RVICPTHGPVWKKELNKVVGIYDKLSRYEGEKGVVIAYGSMYGNTARTAMRMSKELAS
                     LGLETVVMHNLSVSSLSDVLRDVFKYRGLVIGSPTYNAGLFPPVEALVNALAARNVKN
                     KVYACFGSFTWAGAAAKKLQEFGVTMNWEIAGEAVELKQGYSREKTEACALLAKNLLA
                     KL"
     gene            5803..7449
                     /locus_tag="PLI37_00520"
     CDS             5803..7449
                     /locus_tag="PLI37_00520"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOQ57532.1"
                     /translation="MTKSALQIARAEYKPKMPLALLGPVKIKEGAPTESVSDKEEIQK
                     LFPNTYGQPIIEFEPSSQHSSFPPINVGVILSGGQAPGGHNVIAGIFDGIKSLNAESR
                     LFGFILGPAGLIEHNYMELTADIIDEYRNTGGFDMIGSGRTKLETKEQFDRGLLILKA
                     LDIKALVIIGGDDSNTNACVLAEYYKSINAGVQVIGCPKTIDGDLKNEMIECSFGFDT
                     ACRVYSEVIGNIQRDCNSSRKYWHFIKLMGRSASHIALECALQVQPNVCLISEEIEAK
                     NQSLDDVVTEIAKIVARRAEQGNNFGVVLIPEGVIEFIPAMKALIAELNEFLMEYQSE
                     FGLIRKSEQRNYVINKLSPKNSAVFASLPENVAMQLTLDRDPHGNVQVSMIETEKLLA
                     EMITTKLAKWKAEGKFVGKFATQTHFFGYEGRCAAPSNYDADYCYSLGYTASRLIAYG
                     KTGYMSSVRNTTKPAKDWIAGGVPITMMMNMERRHGEMKPVIQKALVDLKGKPFRTFV
                     SKRAAWAIQTDYVYPGPIQYFGPTEVCDQPSKTLQLESGS"
     gene            complement(7470..8765)
                     /locus_tag="PLI37_00525"
     CDS             complement(7470..8765)
                     /locus_tag="PLI37_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOQ57533.1"
                     /translation="MKTKYHSDSPIRIAVVGLGYVGLPLARLFSTKYPTRGFDIDPLR
                     VEAIMQGRDATGELSPEVLAEALGKGFSCSSKLSDIKDCNYYIVCVPTPIDKNNHPIL
                     EPLVQASGLLAKVLKKGDIVIYESTVYPGVTEDICVPVLEKDSGLQFNRDFFVGYSPE
                     RVNPGDRVHSIEKIIKVTSGSTPATADKVDALYNSVLLNGTYKVSSIRVAEASKIVEN
                     AQRDVNIAFINELARIFNAMNLDTKEVLDAAATKWNFIRLSPGLVGGHCISVDPYYLI
                     EKAHIHGYLPRMMSDARQLNNSMGSYVGHRVLKLMNKKGLLVKDARILILGVTFKENC
                     PDTRNTKVVDVYNTLREYTSNIVLYDPHADPADFQKEFGLPLLREGLENRKEKFDVVV
                     LAVAHEAFRKLDIKDLLNKNGLVYDVKSFLPREMVDDRL"
     gene            complement(8808..10682)
                     /gene="yidC"
                     /locus_tag="PLI37_00530"
     CDS             complement(8808..10682)
                     /gene="yidC"
                     /locus_tag="PLI37_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOQ57534.1"
                     /translation="MDKNTLIGFGLILLILVGFSMLQKPTEQKVQQAIQQDSIIQATP
                     QQPLSQQTASYEQELVEAGQTEAQALNDNYGQFAAFLTGENQIYNIENDLMVVSMASK
                     GGRIASVRIKNYVTHDSLPLILFDEKNSSFDITLISSNNRIINTKDLYFTPVQGQNPL
                     EFIFRLALAQDQYLDFVYTLQEDSYLMSMDICGQGLENMLSPSLSNLDLNWAVKMRSQ
                     EKGRKFEQRYSMLQYKFMGDDVEKMSVNKDESKIVNNRLHWIAFKDQFFASVLICDKG
                     LISNELSSKLESDLSPHIKDYKLRSGIDFDMRGNREAKLNFYFGPTKYSILKKFDKGL
                     KKSERLGLDNLVPLGGSIIRWVNVGLIIPMFNFFGSFIGNFGIIIILMTICIKLLILP
                     LTFKSYKSSAKMRLLKPQIDEINTKYAGESKAQERSTATMELYKRAGVNPMGGCLPML
                     LQFPILIAMFWFFPASIELRQESFLWAKDLSTYDAVISWEANIPFISKFFGNHISLFC
                     LLMTITNLIYTKINSDNTGGSNQMPGMKAMMYMMPIMFLFIFNQYSSGLSFYYFVSTL
                     FTIGQTYIFRATIDEEKMLKQLMENKNKPIKKSSFAKRLEEAQRMQREQIRQQAKKRR
                     "
     gene            complement(10687..12291)
                     /locus_tag="PLI37_00535"
     CDS             complement(10687..12291)
                     /locus_tag="PLI37_00535"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOQ57535.1"
                     /translation="MKNTKFIFVTGGVVSSLGKGALSAAMGKLLQARGYRVTIQKFDP
                     YINVDSGTLNPLEHGECYVTCDGHEADLDLGHYERFLNIKTTRANNITTGRIYQTVIN
                     KERRGDYMGKTVQVVPHITDEIKRSIRQLSLKGQYDFIITEIGGTVGDIESLPFIESV
                     RQLTWELGSSNCMCIHLAYVPYLSATKELKTKPVQHSVKLLLELGVQPDILVLRTEHP
                     LTTEIRKKVALFCNVNEKAVIECIDLPSIYEIPLKVRQENLDTIILEKFNLPVNTESD
                     LQPWIDFVNKMKNASEKVHIGLVGKYTSLPDAYISITESLKHASAYSDHCLDLRLISA
                     EKIEENNVEKTLSGLDGLLIGPGYGIRGVEGKISALKFAREQNLPTFGIGLGMQCMCI
                     EFARNVLDLKDATSTEFNPNTADNVIDLMDEQKSVANMVGTMRLGNFDCEVDKNSIAY
                     RAYQNEHIQERHRHRFEFNNNYKERFETAGMKCVGINPDTQLVEIVELSDKKWYLGTQ
                     FHPEYNSTVLNPNPLFVDFIKACINK"
     gene            12407..14134
                     /locus_tag="PLI37_00540"
     CDS             12407..14134
                     /locus_tag="PLI37_00540"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOQ57536.1"
                     /translation="MLNRIIGGFVLLFVLQILPMNAQQSIIPGNSLHFNAVPNFDNYL
                     QPTPEKDSLPKAPSTEIEIEYSLERALLDSLSAILRKSQGLEYDPDSLWFDSDSLWFD
                     SDSLWFDSDTLRFYSDSLGWENALDSLGRILSDTLNLQLSDSLSVDTIPQEELVPKEP
                     ELSPLHRYIRDYDIFKDRVYPKPPMELPEDTVLDYPKMSPLFMPLVFSARKREFKLEW
                     REPKRQVIPESWLQTQELGQSIGIPYVKRLARQILLAFEVEQIDKIRYLNTDLPEPER
                     LEVPLEANRPIQWSAPQRVRPRSIEELPTAPVRYTHWVVKGRLSNQLTQTYVSPNWSS
                     GGVSNMSTLSAFYWVAKYDNKKGIQFDNFIDIKLGFNTVSNDSLRKVNISNDQFQASS
                     KLGIRAIKNFYYSASVDFTTQFLNNYKPNTYDMKAAFLSPAKLYLGLGMDYKIKNKEK
                     GYSFSALLTPFTYRLNYLQNITAFNPKAFGIEEGKHFGQDMGFKIATTIDMQITEFIS
                     WNSYFYFYSDFKYIDSEWKNTFNFNVNHYLSTQLFIHMKMNTKNERPEGEKLIQFKEF
                     LSFGLVYYW"
     gene            14229..14669
                     /locus_tag="PLI37_00545"
     CDS             14229..14669
                     /locus_tag="PLI37_00545"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOQ57537.1"
                     /translation="MDEIRHYGLVESIDEKGLHVRIDQYSACGACQAKNYCSSSGKKE
                     KIIDIFNYQGRYKVGDKVEIQGSTVMGYKAVLWAYVVPLCFTVLILVLSLTVLFPGKE
                     APAALLALFSPLAYYLVLYRFRSALKKEFRFKVRSLEDDVSIEL"
     assembly_gap    14708..14807
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14821..15708
                     /locus_tag="PLI37_00550"
     CDS             14821..15708
                     /locus_tag="PLI37_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HOQ57538.1"
                     /translation="MNVILIAVIVLGGTGIILASVIFLTAKKFWVYEDPRIDEVQNVL
                     PGANCGGCAYPGCRNFAEACVKSESLDGLLCPVGGNDVMKSVASLLGRDAVEADPLVA
                     VLRCNGTCANRPKTSCYEGVSSCMAASLLYGGDTACTYGCLGMGDCVAVCQFDALYID
                     KETGLPVVDEAKCTSCGACVRACPRHLFELRKKGPKSRRIFVSCMNKDRGALARKACS
                     VACIGCGKCEQVCTFDAIAIKDNLAYIDYNACRLCRKCVEACPTNSIWELNFPPRKKA
                     AEQEVSATKVETGKDTTTV"
     gene            15762..17096
                     /gene="rsxC"
                     /locus_tag="PLI37_00555"
     CDS             15762..17096
                     /gene="rsxC"
                     /locus_tag="PLI37_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOQ57539.1"
                     /translation="MRTFSMGGIHPPENKISAGKAIEQVPLPAQVAILLNQHSGAPAV
                     PLVQRGDRVKTGQLIAEAAGYISANIHSSVTGKVLKLDEVYDASGYRCQAVIIEVEPA
                     DEWAEEVVLNSTLKRDCILSPEEIIQKIKEAGVVGMGGASFPSHVKLSPPPGTKPEVL
                     IVNAAECEPYLTSDHQLMLEKGMEIIVGTKITMMALKVNRAVIAIENNKPDAIALLER
                     LAIPYSQIDVMPLAVHYPQGGEKQLIEAVLGRHVPSGALPVVTGAIVLNVGSIFANYE
                     AVQKNKPVIDRVVCVTGKSVKKPGNYLCRIGTSVQDLIDFAGGMPDSTAKVISGGPMM
                     GKALINTQVPVTKGTSGILLMDGNEAHRNEVYNCIRCGDCIEACPMGLEPYLLMSLSE
                     LSEWEAMEKECIMNCIECGCCSYVCPSNRPLLDHLRVGKFKVGAIIRGRKNQ"
     gene            17100..18071
                     /locus_tag="PLI37_00560"
     CDS             17100..18071
                     /locus_tag="PLI37_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOQ57540.1"
                     /translation="MENKLYVSMSPHIHGGDSISRKMWDVVIALIPALLVSLYFFGLG
                     SLLVTSVSIASCVLFEYLIARFLLKKESTLRDGSAILTGLLLAFNLPSNLPLWIVVIG
                     ALVAIGMAKMSFGGLGNNIFNPALVGRVFLLISFPQQMTSWPLPKPFNLHYLDAATGA
                     TPLSVMKSSRFDRLPDMLDMFIGNSGGSLGEVAAAALILGGLFLLIRKVITWHIPVSI
                     LLTVYIFTGILRVANPEVYADPLSHLLTGGLMLGAIFMATDYVTSPMVKKGQVIYGIG
                     IGIITVCIRVFGSYPEGVSFAILMMNGVTPLINAYCKPKRFGEVKKK"
     gene            18068..18706
                     /locus_tag="PLI37_00565"
     CDS             18068..18706
                     /locus_tag="PLI37_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOQ57541.1"
                     /translation="MKKLESSFKNMVLVLGSISIVAAAALGWINELTSEPIRLAAIAR
                     QEKAIRMVSPEFDNQPVEEQFELIDYEGNSLICFPVKKEGKLLGVAIETFSMKGFNGE
                     VRLMLGLKPDGSINNYEVLKQSETPGLGTQMTYWFKTDKNNQSILNKDPGKDKVWVDK
                     DGGDIDAITAATITSRAFLDAVDRAYRAYMEHGGTVVDVVTTATIEEKEAQK"
     gene            18703..19293
                     /locus_tag="PLI37_00570"
     CDS             18703..19293
                     /locus_tag="PLI37_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOQ57542.1"
                     /translation="MKNKVGIILNGLIKENPTFVLVLGMCPTLATTTSAINGIGMGLA
                     TAFVLIFSNMVISLIKNLVPDNVRIPSFIVVIATFVTILELVMQAYLPALHESLGLFI
                     PLIVVNCIVLGRAEAFASKNSVLDSALDGIGMGLGFTFALGLLGMIRELLGAGTLFGL
                     QIIPEDYGMLMFILAPGAFVSLAYLIAIVNRIRKVS"
     gene            19290..19868
                     /gene="rsxA"
                     /locus_tag="PLI37_00575"
     CDS             19290..19868
                     /gene="rsxA"
                     /locus_tag="PLI37_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOQ57543.1"
                     /translation="MIYITIFIAAVFVNNIVLSQFLGICPFLGVSKKISTALGMSGGV
                     AFVLTLSTLITWLIFRYLLEPFELAFLQTISFILVIAALVQLVEIIIKKISQPLYQAL
                     GVYLPLISTNCAILGVAILVIQKEYNLLESVVFAFSTAIGFGLALVLFAGIREQLSRT
                     KIPKGMQGTPIALLVAGLLAMAFMGFSGIVKL"
     gene            19880..20902
                     /gene="galE"
                     /locus_tag="PLI37_00580"
     CDS             19880..20902
                     /gene="galE"
                     /locus_tag="PLI37_00580"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOQ57544.1"
                     /translation="MAASILVTGGTGYIGSHTVVELQQSGHRVIIVDNLSNSNKSVLG
                     GIAKITGIRPDFEQVDCTDFKAMDALFAKYGDIKAVIHFAASKAVGESVEKPLLYYRN
                     NLTSLINLLELMSKYGTEALVFSSSCTVYGQPDVLPVNEDAPIKPALSPYGNTKQISE
                     EIITDSIKAGARYKASILRYFNPIGAHPSAEIGELPLGVPQNLIPYVTQTAAGLREML
                     SVFGNDYNTPDGSCIRDYIHVVDLAKAHVVALDRMLDGKSADKLEIFNLGTGKGLSVL
                     EIVETFQRVTGVKLNYRIVGRRPGDIEQVWADPEKANKVLGWTARETVEETLLSAWRW
                     QQKISS"
     gene            complement(20933..21940)
                     /locus_tag="PLI37_00585"
     CDS             complement(20933..21940)
                     /locus_tag="PLI37_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57545.1"
                     /translation="MKIRLFLLLWLASSFFFIQAQQANPEFKPNGKAIITVFSDFHTG
                     IGAAGKETGFGLERAYLGYQYAFSESLSGKVVFDMGSSKLAGSALERIAYVKNAMLSW
                     KKGNFGLDAGLIGLKQFSVQEKFWGYRYILKSFLDQYGYGYSADMGLSVQYRINDLLT
                     ADATITNGEGYKKLNTDKYFRYGGGLSITPIKELIFRLYYDRHDKDPDNLTHEKSKQI
                     ASFFAGYQNQAFSLGGEYLRMFHSQFRNDRNQHGYSAYTTIKLPKDFQLFGRWDYAAS
                     NKDYDNPLDIWNPGDQQMAILGVQYAPIKGVKLAPNFRMVAPKNDDALYYITVNLSLS
                     L"
     gene            complement(21955..23367)
                     /locus_tag="PLI37_00590"
     CDS             complement(21955..23367)
                     /locus_tag="PLI37_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ57546.1"
                     /translation="MHYKTRLYLNFGLIALAFMFITILFQLDREKRYKTEEIRSKLAA
                     YSEMIDAYLLQNCDTIEVTHILPEDLRFTIIDSEGSVIFDNVIGHIEDMSNHANRPEI
                     LSARTKGSGYAIRRSVSTQTDYLYYAHRCNTGHYIRLALPYNVKLSDVMKQENLIFYG
                     LAVLFIVVLALLLYKADKFSRVMHSLRRFTSDIEKGTVDYKSFQFPDSDSGIIGKKII
                     SLYEQLEKSKQQTDLEKERNRLIKQEMTNNIAHELKTPVSSIQGYLEILNENGDLEST
                     KRKYFVERAYAQTLRLSALIKDIALITKLEEASNLFPREKVNIGRIAEDVINELQAQL
                     DKAGMKVENRLGADLEISGNYNLVHAIFRNLVENSINYAGKNSSIVVECSKEDTEFYH
                     FKYYDTGYGIKEKYLQKIFDRFVRLEESRDRRTGGTGLGLSVVKHAVLFHQGNIIAVN
                     RPEGGLMFKFCLKKHSAVRI"
     gene            complement(23395..24081)
                     /locus_tag="PLI37_00595"
     CDS             complement(23395..24081)
                     /locus_tag="PLI37_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ57547.1"
                     /translation="MKRILVVDDEQDICEILQFNLENAGYEVVSANSAEQALELLLDK
                     NLNYDLLILDVMMGGMSGYQLARKIRTEMNSQLPIVFLTAKNTENDLLTGFSLGGDDY
                     IAKPFSIQEVLARIKAIFRRSESNKPSGDEEILKSRGLILNRKAKTASLDGETLALTK
                     KEFQILDLLMHSADSYFTRGQILDSVWGDESYVLERTVDVHIARLRKKMGAAGEYLQS
                     RPGFGYRWLS"
     gene            complement(24094..24762)
                     /locus_tag="PLI37_00600"
     CDS             complement(24094..24762)
                     /locus_tag="PLI37_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HOQ57548.1"
                     /translation="MDTIRETYLARLTQDFKAMANLVLRQAQILKLQLAGDTTQALKD
                     EMQKNEHSIDDMEIKLRDDIINSIVLYTPRAGDLRRIVSYYDTVIDLERIADTMDSIS
                     RRMQYLNRQDSIYPEYQKYAVNLFEFADSMLQNAIFAFLWADNTMARSIINSDEELDK
                     LHHLSHKKLFEHEKTDKDHPYWIADILDLGRILYGMERIGDGATNIAEAVIFLSEGRD
                     IRHG"
     gene            complement(24773..25546)
                     /locus_tag="PLI37_00605"
     CDS             complement(24773..25546)
                     /locus_tag="PLI37_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOQ57549.1"
                     /translation="MENLNKTPILSLKKLCVSYTPGKFAVNNVSADIYEGLITSVMGP
                     SGCGKSTLIRAINRMHELYPDIRISGNIELKGRDLLHINPSEVRRMAGMVFQRPNPFP
                     TMSIYDNVLAGYKLIGIKQSRHEKDELVESSLKSVGLWDEVKDGLSKRGSFLSGGQQQ
                     RLCIARALALKPDILLMDEPTSALDPIATNLIEELLVKLKKEVTIVIVTHNMTQAARI
                     SDYSMFMYLGELVEYDRTEIMFTNPSDKRTEEYLTGKFG"
     gene            complement(25534..26394)
                     /gene="pstA"
                     /locus_tag="PLI37_00610"
     CDS             complement(25534..26394)
                     /gene="pstA"
                     /locus_tag="PLI37_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOQ57550.1"
                     /translation="MSSLKYRLFKNKLLTLSVVIFSALTAVPLVLIIGKVFLKGWRQI
                     NWDFFTKTTPTTLDALLAINNNQPIPGGIANGIVGTLMMVLVASVIAIPLGVMCGIYL
                     SENRKTKFSTVVRFLTDLLQGTPSIILGIIAYLWVVIPLKGYSALAGSVALAIMMLPL
                     IVRSTEETMNLLPASLKEAGLALGSPYRSVVLKVLLPSAFGGVFTGILLAVSRVIGET
                     APLMLTALGATTIQWDITKPASAVSLLIWEFYNDPNLQSMIWSASLFLLLLVLSLNIL
                     AKQLAKKWRI"
     gene            complement(26391..27251)
                     /gene="pstC"
                     /locus_tag="PLI37_00615"
     CDS             complement(26391..27251)
                     /gene="pstC"
                     /locus_tag="PLI37_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOQ57551.1"
                     /translation="MSDKIFRSLLLVSALILLLIFAGMFFSLSKGAVPAFREFGFFGF
                     IFSDKWISGTGQSTEQYGALAFIAGTLMTSVLALLICLPFSLPVAFFVGEYFRGKKVA
                     GVLGGIVDLLAGIPSIVYGLWGFYALRPLIQSLGLSEQGFGVLTSSLVLAIMIIPYAA
                     SLSAEFIRMVPQELKEGAYSLGATRAEVIRNIIFPTSFSGIFSAYVLALGRAIGETMA
                     VTMLIGNTSRIPRGLMDTGNTMASIIANQFGEADGLKLSSLIAIGLLLFLITAIVNFL
                     GRLMIKRHSV"
     gene            complement(27264..28289)
                     /gene="pstS"
                     /locus_tag="PLI37_00620"
     CDS             complement(27264..28289)
                     /gene="pstS"
                     /locus_tag="PLI37_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HOQ57552.1"
                     /translation="MKKAIFLLAVSSLLLISCSQKTSLSGAGATFPLPYYNLVFKAFT
                     ENAGIDVSYGGIGSGGGIRSLKDEVVDFAGSDAYLSDEEMAEMKATIHVPTCLGAVVL
                     SYNLPDVSSLNLTGDIIAGIYLGKITRWNDAAIQAINPAVSLPDKAITPVYRSDGSGT
                     TFVFSDYMTKINKDWENTLGTGKSLNFAHGIAAKGNPGVAGTISQTEGAIGYIGSEYA
                     FALNSPIAYMQNAAGEFILPSSLSISAAAQEGIADDTRSMITNSSAKGSYPISCLTWI
                     IVYKDQAYANRSEDQARATLSLLNFILSPAAQAQTTMVHYAPLPKEVIDKAQKALSEI
                     TYNGKSL"
     gene            complement(28408..>28654)
                     /locus_tag="PLI37_00625"
     CDS             complement(28408..>28654)
                     /locus_tag="PLI37_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57553.1"
                     /translation="LLLVFLLLMSRCRPCEYQTISTLSLLDSLRVGNSIYRGVMHFCC
                     LELAEEWEDHTVKEIYLAKNYGPIQYKLQNGLVYSRD"
     assembly_gap    28655..28754
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28755..>30014)
                     /locus_tag="PLI37_00630"
     CDS             complement(<28755..>30014)
                     /locus_tag="PLI37_00630"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HOQ57554.1"
                     /translation="KLAWTAGNFGPFTDELEAGVPVPDVTYPRFIEKPDGNLLFECRY
                     KLSGDGDSYLREYDGDTHNWSLVGRYIQGMDTNPNTCAYINRMDYDKQGRLHVSWCWR
                     DDFGGQTNHDLCYAYSDDHGRSWKDTYGNRVATSEAINYTDSRSPGNCLRQGIATLKI
                     ADIPHNKGYINQETQSCDSKGRVHIVNSYMDSGTDSNWSSSRSKAVLHHRFRDTDGNW
                     HHNYIYNKGQKVNSYCRVQIILDAFDNAYVVANGAEIYAATSAAAYSDWELVSNLDAG
                     RFCSEPQIDRNAIDDGLLSFVYLARNNELSIIDYLLDNPKPATGSGLRVYRYSDNRFS
                     NCIDSASGLPGSPLNTNVKSLRWSGCLETRYGEAYSLYLNTTAASRLYINGRILLITP
                     NTKKPVEYAVELPPFNSHRHNIVIESLA"
     assembly_gap    30015..30114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            30253..34257
                     /locus_tag="PLI37_00635"
     CDS             30253..34257
                     /locus_tag="PLI37_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOQ57555.1"
                     /translation="MQISFEYFPYMDQLPSSSVRRVLQDSEGYMWFGTLDGVCRYDGY
                     QVRTFRSDMNNPSLLTNNDIQSLAEDKQNRIWIGTKQGINLLDRNTLNITPFDESPVQ
                     GDNINALACDDKGNMWIATPRGLFCYDNTRNCLKTYWSDPYDEKNLPDQTISHVSKDK
                     QGNVWIMFWNGGLCRYLPESDNFERFPPTKEMSGPFRLYQDKNDQYWLCTWGSGFFKF
                     DPEAPPENRYTRIPVYNTAGEEENILFSMVQDDRYGYFWLMSLTGLYVFEYDEQNTRL
                     IQVDISEHLIGSSKLYSEIIKDKDNNLWIGAYSEGVIFVNFDLPNVRNYGMEVLRQKL
                     GFPVSIRAMSEDSDGLVWLGVNRYGVFFYDRHENRVFSIDEIPTIENHQELSNLKTVN
                     YIREIKSRGEHWICCDDQWIYIFRKDGRGVYYAGRLDIDVSNELNWASGKVLFEDKDS
                     CVWVGMLNQGLVKISPENRVTMVEPIPMVTGITQDLHGDIWVSSEKVGLCCLRPFGET
                     YRLIRYDKYSEGLNTNNIQSVCADLSGKLWLGTKEGRLLTFDKNTGSFEDMSPACAMT
                     GESVLNLLVDRNNNLWISTNKEVTGFNHRTLTSTSYSISDNLVVKSFIKGSYSATSTE
                     EVLFGGNKGFCAFSPGKQHNDSLKSKSVRVHITDIKVHNQSVLSNNDEHDYNPELKRL
                     ILGPAERNVEFEFSSLNYAAPFKVQYAYKLEGLDSEWTKVNATRRFALYNNLHQGKYK
                     LMFMATDENGRWSEPLPGLEIIKRPPLYLSWWAILLYVVLLILLFYALIRFFTNRVRL
                     NQQLNIAQIEKAKSEELTQTKLRYLTNVSHELLTPLTVISCLVEDVDQVSKGEYWQHE
                     VMKVNLSRLKRLLQQILDFRKVESGSMKLKVAESNMIEFVERICAFNFVPLAKERDIR
                     FELQLPDYPVQGWYDPEKLDIILFNLLSNAFKFTPEGGSVRLSLSLLQKSKGRVAQLI
                     VSDTGRGIQPDELEQIFTRFYSNDSSLTVENHGIGLTLTKELVEMHHGSIRVESEPGK
                     GSTFILEFPIEKSAYAPEELVAETTEEEAVLSDQASDTGKSQASTKHKEDVHILIVED
                     NAQLCDTIEAILNRNYHTYKAFNGKEAVALINSRHIDLVVSDIIMPGMDGLELCRTIK
                     NNLDTSHIEVLLLTAKNSINDRIESYNAGADGYLSKPFELKVLQAKINSLIRNRKRKM
                     DSFKHNAEINISSLEYTSLDEKFLENAVSIVEEHIAVTDFNLDLLSSRLNMSKSSLYR
                     KIKSLTGLSPVEFTKNIRLKHACQMLTDRAGNISDIAYAVGFADPKYFTSCFKAEFGL
                     TPTEYIKKIKKEAR"
     gene            34447..35538
                     /locus_tag="PLI37_00640"
     CDS             34447..35538
                     /locus_tag="PLI37_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOQ57556.1"
                     /translation="MLSCRAKDRDCIRPGEIWPDNNGVHINAHGGGMLYYRGQYYWFG
                     EDKSERSNAALKGVRCYSSKDLCHWKDEGIVLAVVEDEPAHELTKGCIIERPKVVYNK
                     KTGTFVMWFHHELRWRGYSAARVGLAVSDKVTGPYRFIHSYRPNPGIWPENMSEEDKS
                     LDMEEASRLESWTPEWLEAVRKGFFVIRDFEGGQMSRDMTVFVDDDSKAYHIYSSEEN
                     LTLHIAELSDDYQSHTGKYIRIIPAGHNEAPAIFKKDGRYFMITSGCTGWRPNAARLL
                     TATSIWGPWEQKPNPWQGEQADISFDSQSTFIFPLAGKKDAFVFMADRWRPRNPSDGR
                     YIWLPLEFEDGLPVLRWRDSWSPAKAFKK"
     gene            35547..37346
                     /locus_tag="PLI37_00645"
     CDS             35547..37346
                     /locus_tag="PLI37_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ57557.1"
                     /translation="MKKSLLIYLLILGGFSLYGRGTAPYVLQNVYARNYETLNGSWNH
                     IVDPFDNGYYDYRMNINRSGFFRNQKQANPSDLVEYNFDTSPCLQVPGDWNTQDDRLF
                     FYEGSVWYKRDFNFEPREGERQFIYFGAVNYFCNIYLNGREIATHEGGFTPFSVEVTD
                     KLRKGHNFLILRVNNDRRPENVPTVNFDWWNYGGITRDVLLVSLPKLFVDDYSLRLKK
                     GSYRELEGYISLSDPQAGVELSLEIPELKIKQTIQTGEQGRADFNIKAKPELWTPQNP
                     KLYRIILRQGNEVIEDNIGFRHITTEGKKILLNGQQVFLSGISIHEEAPYRQGRVYSK
                     EESVLLLSWAKEMGCNFVRLAHYPHNEHMVRAAEEMGLMVWSEIPVYWTIAWDNPDTY
                     QNASNQLRDMMERDKNRCAVVVWSVANETPHSEARDKFLIGLANQVREKDDSRLLSMA
                     MEVTGQGNNISRVRDNMSDYVDIISVNTYLGWYGGSLDAILSRTWEIPYDKPVFFSEY
                     GAGALQGLHGEENVRWTEEYQERLYKNTIEMFDKVEGFSGMSPWILMDFRSTRRQLRG
                     VQDFFNRKGVISERGIRKKAFYVLQNYYKSKDY"
     gene            complement(37474..40785)
                     /locus_tag="PLI37_00650"
     CDS             complement(37474..40785)
                     /locus_tag="PLI37_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOQ57558.1"
                     /translation="MHCRLFSILFTIILFGCSHPNQKFGRHSLADAVDYFATVPDSTR
                     TKTWWFHGETETTKEGITADLEAFKKAGIAGVVYYDQSHGKAPDALDGFSDKWWEMLC
                     FAAREAERIGLSFEIHISNGYVAGGSWISNEYAMKRLASTELMIDGGRYFEGVLEAPL
                     NKYNFYKDVAVLAFPAPNGVGISSSNRPVKLTSNKAEIDLKNLFDPKSDRLSKIPFSP
                     DGKPVYINLEFDEPFTARSITYQVRPKGKATTSATNIPGPPQETFVGTGYRILPDLGQ
                     LEVSQDGINYSKVCDLKPVYRAHESWRQKTISFAAVEGKYYRLNLHDWWEKDEKTPDD
                     MQIGNLVLHAAAKVDQWEEKAGFFSEYIEKDKTPDYPASEAIDLSKIINLTDKMLPDG
                     TLKWEVPAGRWMVMRFAYVPTGGSTKHGRKNLMGRECDKLSVEAAEYHWKHYVQVIID
                     SLKSSNSGILSGVAMDSHEAGAQNWTDNFIQEFTSRQGYDPTPYLPAMMGYVINDVET
                     SNGFLFDVRRNIADMISDNYYGSFERLCNENGLIFTAQAIGNALCIVGDPIQAKSKVS
                     KPQGEFWPIHPDGNYDIKESSSAAHLYNKPIASAEAFTDAKYYHSLSDLKSYADYAYA
                     FGINEFVICASPYQPWLDKIPGSTGGGRQYAINRNNTWWDYSEPFWNYQARNAGILRL
                     GKPSIDLCVYLGENAPVKILTYRLPDIPGGFDFDAFTSHALHTRMSVQNGLINLPGGV
                     NYKMMILPRNSNIPYKALEKIAEMVKKGIKVYGDKPTFSESHRDREMRPAYQKLADEL
                     WGINPNNEGSNTVGKGTVYWGMPLSEALNQAKIKPDISMETGNTKSNKIYFTHRSLSN
                     AEIYFLDNHKDEPEENLFSFSASGKQAQLWNSVTGKRFSIPVSNHNGNSLTIPLHMAA
                     RESFFVVITDIYEDLPPIVWTESDKDTTLLNDNWNVFFDPGLGGPGEVVFDTLHDWTK
                     NNEAGIKYYSGTAVYKRNFYAEQSDKRTFIDLGNPHFVAKVFVNSNEAGIVWCSPWQL
                     EISDYLKEGENTLEIHVANSLINRMVYDATLPEEQRITYSYPQIMKPGDDLMASGLKE
                     VKIIRFLEQQTLGEQ"
     gene            complement(40971..>41528)
                     /locus_tag="PLI37_00655"
     CDS             complement(40971..>41528)
                     /locus_tag="PLI37_00655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOQ57559.1"
                     /translation="NILVIPIGKKIAQASVKMGYNIPSGFENLDFILDKPNAKVVLDL
                     ANQLKQLYLSGKVDKIELIYHHAKSKSSQVLEARTLLPLDLKERDVIEKPVVRERIYE
                     DDFIIEPDRENLLHDLVPYIINMRLYLALLDSSVSEHTARMLAMQTATDNADELLEEI
                     KLQYNKLRQQAITSELLDIVGGSME"
BASE COUNT        10683 a         9149 c         9900 g        11496 t
ORIGIN      
        1 tagtatttaa aataaaacct gtcctgtgga tgcggtgggc ggacgaatat acaacattcc
       61 gcagagggta ggagtcaggt caaaaatgga aacaagctga ctgtggttac tggccttgat
      121 tcccgctccg taaaagacca gtatcgtggt ttggttagca tattgtattt gccggttttt
      181 gcccgggtag gattcccagc ggacgttgcg tccgttttca agtttcagca gtaaatcgcc
      241 ggagcgttct ttgtgataat tcaggctaag agccgattca ctgctccaat cgcccagcag
      301 aaagcagtaa gcgggtacca cctgttgtat accctcgacc tgggtcagaa acagagccgc
      361 tttgttgcag atttcccggt aatcgaggtt gagctcattg atgcgtttgc ggttgaggta
      421 aatttcgttt tccgtgcagc cttgtaccca gcgttcgctg ccgtaaatag ctgtcagata
      481 aaggtttagc aaggcggtag tgcgttccgg ataatagtta ccgtaaaatc ctggagcctt
      541 tgggttttct tccacagcca gcctggtcgc tccggtaccg cagagataaa tcaggacatt
      601 gtcgagtcct attttccggt cgatttcttt gagtaaccag cccagatcct ggtccaatct
      661 gaaatacaga tcctgcattt caagggcggc ccggctgtcg ttgccgctca gttggctgcc
      721 ggtgttgaaa tgcaatatca acaggtccgg gtattcgtct tctcccagct tttcttgatc
      781 gataagctcc agagccaact gattgagttc ggtgttggcc atgggggttt gtttgaagtc
      841 tctgtaaccc gtaccgttga taaaagagag agcgtaactg aaaaagcccg gatttttttg
      901 gtgaggcata tacacatagc ggctcagagg aaatttcggc ctccagccgt ttttgccgtt
      961 gtttgtttcg aacagtccgt aatcgttgat tttttgaagc cacgacggca tataattgta
     1021 ataggtgcta ctgcaccatt tgccactggc atcgtccatc cagagggctg catcggcgct
     1081 tcgccctccg gcaacaatgg cggcctcggc ttccagccct atcgaaaaga ctttggaacg
     1141 tcctttgcag gcttttttga gttcatcgcc caaggtattg cttctcaaac tcaggggcga
     1201 aaaggttgaa gtggtgtaat ttcccaggta atccgtatcg tagacgcagg acacgggcct
     1261 ttcgagttgc cggtcgtacc attcacgggc aatgatgccg tgatgcatgg gagcgcagcc
     1321 gctcaacagg caggcgttgg tgttagccgc atcgggacag tttaaaccgt agttgcaatt
     1381 attgtatact ttgccccgtt ccagcagtaa cttgaagcct tcctcgccaa atccttccat
     1441 aaaccatttc aggtattcct cctgtaactg atccaggtgt atgcccaata ccaggcgggg
     1501 cggttgtttg ctgtctttag gagcggaaca gacctgagaa ctcaggctca acatcaacaa
     1561 aatagggagt atatgtgaaa atgacttcat agatttttac cggatttgcc tgagccttgc
     1621 ccgggcactt ttttgttacg aatgctcttt ggaagaggat tgatccaggc caacaggcta
     1681 cgcgacaata aactcagcag aagcgggatc atggccggat gtacgtattg cggcaaaaag
     1741 ccgaaacctg ccagtgcaaa aactaaaacg ggagtattta tttttaagta gccattgtaa
     1801 aaaatgcttc gcctgaaagg cttgaatgcg attaacaaag caaaaagcaa atgcaggggg
     1861 tgcaaccaga gcagaaggta attggttttg gtaacgggat gtaccgaaat taaggccaag
     1921 aaagcaataa tcaagcccgc aatccctgcc agtccaaaaa taagggcgtt taccagagga
     1981 taccggcagg ctgttttgag ttcccgcagc cccagggtca gaaccagcgt aaacaaaatc
     2041 caactgaatg ttacaggtcc gggcagggtg tttttttgtt cggccggcac tgttttgatt
     2101 tcgttcaata tgctttgttc gcttatcagg caacgctccg cttgaaggct gtctttaccg
     2161 ttcgttgcag gagctgtgat tgtggccttt tcaaaagctt ccatcatcaa ttcgggagcg
     2221 aaaagcagat cgagggacgg agccggagca tcggccagac tgctcagtgc aagggaaata
     2281 ccgaaccagg tccagggatg ttttgcggtg taatgttgaa ttacttcacg catgctgccg
     2341 taggcggcct cgtagtgata atcgacggct ccgtccaggc atcgttcaat tattttgcgg
     2401 gcccgggtag agcaattgtc gtagtaaaaa ttgtagcgat aagtccggtt ttcgggtagg
     2461 ctattgataa ccagagcctc ccatagtttt gctttttctt cttgcgagag gttcagcttt
     2521 tgagaaatca cccggcgatt ttcgttgcgg tagctgtgca taaaaccttc gaaactgctg
     2581 attcccaaaa tatagtcgat ttctccccgc acaaaattcc acaggaagtt gtcggagttg
     2641 aagtcaaaaa taccgtaatt gaaaaccaca tcgcggcggg tcggcggatc cagcacccgg
     2701 atgccggtat gtccgtaatg cgcccagact tcctgcccgg gagagcaagt gagcaggtaa
     2761 atctctgcct gtccgctgag tgcaggaact gtttgtgctt tgacaccggg gctgataccc
     2821 acaaagaaca acattcccat tacgaataaa atgagggatc gttgtgcttt gttgatcaaa
     2881 gaaaaaatcg atttacggct tgctgacatc ggtctcttaa aaatcaggca aaaataggca
     2941 attttagcga gattgtttgt atgatatgga acttgttatg ccttaaaaaa agcagtttag
     3001 gttttgatat tccgataaag ttacgaactt tgcgagtcta atacgggttt cggcgaagat
     3061 accaacgaaa ccgataaaca gttataatgc cggcaaaata tgaaaattga ttacagccga
     3121 ttgggagaat gggtacagaa caacactctg cttatggtat tcttgctggg ttttgttgcc
     3181 atactgctct ttgtctttct gattgttcgt tacaaaaaca acaaaaaccg tttgctgttg
     3241 gcccaggaat acctggcagc aggaaaggaa attcctcgtt atttgctgag agaaaacagc
     3301 ctgtacgcca gggctgttaa gaatcttttt ttgggtatcg gcctggtctt ttttctgggc
     3361 tttctcaccg agacctgggg ctgggcttcg gccggcttgc tgtttattgc cctgggagtg
     3421 gggcaaatga tcgtcttcaa aaaatccagc aaagatttta ccggcagcga tttagaccgc
     3481 tgatttcgat agctttattt attttccctt tctcactgtt tttggtaaac tctgacgtaa
     3541 tcgatctcgt agaacaatgg aaaggcagat tcgtcgggtt ttccgccgtt gctgcctatg
     3601 gccaggttta gcagcaggta gtgttcttgc cggaaaggat tgctgtaatt acctatgctg
     3661 ccgttgcttg tacggtctaa gggaatttcg ttgagtaatt cgtcgtctag atagagcttg
     3721 atggcttgct cgtcccaatc catacgccag atatggaatt tggagcccca gtcgggatcg
     3781 gcgttggtga aatgattcaa aggataaacg gaagaatccc aaatagcctt gtagcgctgt
     3841 tctcctcccc aggctgcgtt ggccagaata tggggttctc ctttgatgcg ataatactcc
     3901 atgatgtcta tttcgccgca cgaaggccat tccatggtat tacccagggt ccagatggca
     3961 ggccaggatc caccggccag cggaatccgg gctcgaactt cgaagcgtcc gtaaagaaaa
     4021 gatctcttgt tccgggtagt gatagaggaa gaagtgtaat cggcttgctg tctggaagtt
     4081 cgccaatctc gcgcctgggg attatagcgg ggattgggaa ctttctcctt gcgtccttca
     4141 ataatcagga ggcctcccct gcaataggcg ttgccgggct ggtaccactg caattcctcg
     4201 ttgcgtacaa atccgcgttc gtaattccaa ctgccggaat cgggtggccc ttctgtgttg
     4261 aattcgtcgc tccagactaa tttccattcc tgcgcctgaa gtgttccggc aaggctaaac
     4321 agcaggcaca aggcacaaaa tctcaatctt tcacaaaatc tgatcgtttg catatgatgt
     4381 ctttttttat gggacaaaaa taggttatat ttacggcttt tttaaaaaat gtatttattg
     4441 ggatgattat tgaaaggagt tcttgctctt gtctgtttag agcggaaaaa ttaactttgc
     4501 aaaaaataat aattcaatgt ccgaaaaatt taaaataaac cgctttgttc actacgtagg
     4561 cgtgaacgac cgcaaaactc atctttttga aaatctctgg cctttgcctc tgggtgtttc
     4621 gtacaacgcc tatttggttg ttggagaaaa aatagctctt atcgatacgg ttgaatcctg
     4681 ttatgcttcg gaattgctcg aaatgatagt ccggacggtg ggtaatcgtc ctttggatta
     4741 tctgatcatc aatcatatgg agcccgatca ttcttcttcg ataaatgcca tacgtcaggc
     4801 atatcccggc gttcagatcc tgggtaatgc caagaccata caaatgctcg aaggctatta
     4861 cggagcacac cagggaatta tcgaggtaaa agagggtgac agcctttgcc tgggcggcga
     4921 aaccctgcgt ttttacctga cccctatggt acattggccc gaaaccatga tgacctattg
     4981 cgagcaggaa cagttgcttt tttccggcga tgcattcggc actttcgggg cccttaatgg
     5041 tgccgtgttg gatcaggata tggacctgag tccttattgg gacgaaatgt accgttatta
     5101 tgctaatatt gtcggtaagt acggttcgcc ggtgcagaaa gccttgcaaa aactttcggg
     5161 tctggatatt cgggtaattt gccctaccca tggtcccgtc tggaaaaaag aactcaacaa
     5221 ggtcgtcggc atttacgata aactcagtcg atacgaggga gaaaaagggg tggtaatagc
     5281 ctatggaagc atgtatggca atacggcccg aacagccatg cgcatgtcga aagagctggc
     5341 aagtctgggt ttagagactg ttgttatgca caatctgtcg gtaagctctc tttcggatgt
     5401 gttgcgtgat gtgttcaagt accgtggtct ggtgataggt tctcctacct acaacgccgg
     5461 gctgtttcct cctgtagaag ctttagtgaa tgctctggca gcccgtaatg tcaaaaacaa
     5521 agtctatgcc tgtttcggct cttttacatg ggcaggtgcg gctgccaaaa agctgcagga
     5581 atttggtgtc acgatgaact gggaaatcgc cggagaggct gtagaattga agcaaggtta
     5641 cagtcgcgaa aaaacagagg cttgtgccct gttagccaaa aacttgttgg ccaaacttta
     5701 agtgttttat aaaaattact atttagccct cgccggcttg tgagtgaaca aaaaaaacac
     5761 gaaatttgtg tcgttttttt acgaacttta tttattccct ttatgaccaa aagtgcttta
     5821 caaattgctc gtgcagagta taagcccaaa atgcccctgg cattgcttgg gccggtaaaa
     5881 ataaaagaag gtgctcccac agaatcggtt tcggacaaag aggagattca aaaacttttt
     5941 cccaacacct acgggcagcc cattattgag tttgaacctt ccagtcaaca cagtagcttt
     6001 cctcccataa acgtaggagt tattttatcg ggcggtcagg ctcccggtgg gcacaacgtc
     6061 attgccggca tattcgacgg cattaaatct ctgaatgccg aatcccgttt gtttggcttt
     6121 attttgggcc ctgccggttt gattgagcac aattatatgg aactgacggc tgacatcatc
     6181 gatgaatatc gcaataccgg cggtttcgac atgataggct cgggccgcac caagctcgaa
     6241 accaaagaac aattcgacag gggtttgctt atactaaagg cactcgacat aaaagctttg
     6301 gttattattg ggggagacga ttccaatacc aatgcctgcg tgctggccga atactataaa
     6361 tccatcaatg cgggagtaca ggtaataggt tgccccaaaa ccatcgacgg cgatcttaag
     6421 aacgagatga ttgaatgctc ttttggtttt gataccgcct gcagagtgta ttccgaagtt
     6481 attggcaata tacagcgcga ctgtaattca tcgcgcaaat attggcattt tatcaagctc
     6541 atgggacgtt cggcttcgca tattgcgctc gaatgcgctt tgcaggtgca gcccaatgtc
     6601 tgtcttattt cggaggaaat cgaggccaag aatcaatctc tcgatgatgt agtgacggaa
     6661 atagccaaga tagtagcccg ccgggccgaa caaggaaata attttggtgt tgttctaatt
     6721 cccgaaggcg taattgaatt tattcccgcc atgaaggctt tgattgcgga gttaaatgaa
     6781 ttcctgatgg aataccaaag cgagttcgga ctgattcgta aatccgaaca acgcaattac
     6841 gttatcaaca aactgagccc caaaaactca gccgtatttg cttcattacc cgaaaacgtg
     6901 gctatgcagc ttacgctgga ccgcgatcct cacggcaacg tgcaggtttc tatgatcgaa
     6961 acggaaaagc tcctggccga gatgataaca accaaactgg ctaagtggaa agccgaaggt
     7021 aagtttgtgg gcaagtttgc cactcaaact cacttcttcg gttacgaagg acgttgtgcc
     7081 gctccttcca actacgatgc cgactattgc tattcgttgg gttataccgc ttcacgactg
     7141 attgcctatg gaaaaaccgg ttacatgtct tccgtacgca acactaccaa accggccaaa
     7201 gactggattg ccggcggagt acccatcacc atgatgatga atatggaacg tcgtcacggt
     7261 gaaatgaagc ccgtaatcca aaaagccttg gtagatctta agggtaaacc attcagaact
     7321 ttcgtttcaa aacgagccgc ctgggccatt caaaccgatt atgtttatcc cggtcccatc
     7381 cagtattttg gtccgaccga ggtctgcgat caaccgagca agactctgca actggagtca
     7441 ggcagttaat tgaatcaaga aagtgttgat taaagcctgt cgtcgaccat ttcccgcggc
     7501 aaaaagctct tgacgtcgta gaccaatccg tttttgttga gcaggtcttt gatatccagc
     7561 tttctgaatg cttcgtgagc cacagccaac actacaacgt cgaatttttc tttacggttt
     7621 tccaggcctt ctctgagtaa ggggaggccg aattcttttt ggaaatcggc cgggtcggcg
     7681 tgggggtcgt aaagcactat attggaggta tattcgcgga gggtattgta cacatccacc
     7741 actttagtat tgcgtgtatc gggacagttc tccttgaaag tcactcccag aatcagtatc
     7801 ctggcgtctt tcaccaacag gccttttttg ttcatcagtt tgagcacccg gtggcctacg
     7861 tagctgccca tactgttgtt aagttggcgg gcatcgctca tcatgcgggg caggtaaccg
     7921 tgaatatgcg ccttttcaat cagatagtag gggtcgacgc ttatgcaatg gccacctacc
     7981 agtccggggc tgaggcgtat gaaattccat ttggtggcag cggcgtccag tacttctttg
     8041 gtgtcgagat tcattgcgtt gaatatcctg gccaactcgt taatgaaagc aatattgacg
     8101 tcgcgctgtg cattttcgac gattttggag gcttcggcca ctcgtatcga cgacactttg
     8161 taggtgccgt tcagtaatac ggaattgtac aaagcatcca ctttgtcggc tgttgcggga
     8221 gttgagcctg aggtgacttt gataattttc tctatgctgt ggacacgatc gccggggttg
     8281 acacgctcgg gagaataacc tacaaaaaaa tccctgttaa actgcaagcc tgaatctttt
     8341 tccagtaccg gtacgcaaat atcttcggtg acgcccggat aaacggtaga ttcgtaaata
     8401 acaatatccc cttttttcag cactttggcc agcaagccgc tggcttgaac gagcggttcc
     8461 agtatgggat ggttgttctt atctatgggc gtgggaacgc atacaatgta gtaattgcaa
     8521 tcttttatat cggacagttt ggaactgcag ctaaatccct ttccaagagc ttcggccagt
     8581 acttcgggac tcaactctcc ggttgcgtct ctgccttgca tgatggcttc cactctgagc
     8641 ggatcaatgt cgaatcccct ggtcggatat ttggtagaga aaagcctggc cagaggcaaa
     8701 cccacataac ccagtccaac gaccgcaatg cggatagggg agtctgaatg gtattttgtt
     8761 ttcatggccg aaaattgctt gaatggatgc ccgggcatcc gttattttta acgccttttt
     8821 ttggcttgtt gtctgatttg ctctctttgc atgcgttgag cctcttcgag tctcttggca
     8881 aagctcgatt ttttgatggg tttattcttg ttttccatca gttgtttaag catcttttct
     8941 tcgtcaatcg ttgctctgaa aatataggtt tgtccaatgg taaacaaagt tgaaacgaag
     9001 tagtagaagc tcaacccgga ggaatattga ttgaaaataa acaggaacat tatgggcatc
     9061 atgtacatca tggccttcat accgggcatt tgattgcttc cgccggtgtt gtcgctgttt
     9121 attttggtgt aaatcaggtt ggtgatggtc atcagcaaac aaaacaagct gatgtggttg
     9181 ccaaagaatt tactgatgaa cggaatattg gcctcccagc taatgacggc gtcgtaagtg
     9241 gacaagtcct tggcccagag gaaactttcc tgccttagtt cgatggaagc aggaaaaaac
     9301 cagaacatgg caatcagtat ggggaattgc agcaacatag gcagacagcc tcccatagga
     9361 ttgactccgg cgcgtttata gagctccatg gtggcggtgg aacgttcctg agctttgctt
     9421 tcgcctgcgt atttggtatt gatttcgtcg atctggggct tgagcaaacg cattttggcc
     9481 gacgacttat acgacttaaa ggtcaggggc aggatcagca gtttgataca gatggtcatc
     9541 aaaatgatga tgataccgaa attaccgata aagctcccga aaaaattgaa catgggaatg
     9601 atcaggccca cattgaccca acggataatg ctgcctccca ggggaaccag attgtccaga
     9661 ccgagtcgtt ccgatttttt gaggcctttg tcaaatttct tgagaatgga atatttggtg
     9721 gggccgaaat aaaaattcaa cttggcttcc ctgttgcctc gcatatcaaa atcaatgccc
     9781 gaacgtagtt tgtaatcttt gatatggggc gacaaatcgc tttctaattt agagctcaat
     9841 tcattggaaa taagaccttt gtcacaaatc agtacggagg cgaaaaactg atccttgaaa
     9901 gctatccagt gtaggcggtt gttcactatt ttgctttcgt ctttgtttac actcattttt
     9961 tcgacatcgt ctcccataaa tttatactgc agcatggaat aacgttgttc aaatttccgg
    10021 cctttttcct gactgcgcat tttgacggcc caattcaggt ccagattgct cagactgggc
    10081 gagagcatat tttcgagtcc ctgtccgcag atatccatag acatcaaata gctgtcttcc
    10141 tgcaaggtat acacaaagtc aagatactga tcctgagcca atgccaacct gaaaataaat
    10201 tccagagggt tttgtccctg cacaggagtg aaatagaggt ctttggtgtt gataatacga
    10261 ttgttggagc ttataagggt aatatcaaac gaagaattct tttcgtcaaa cagaatcaag
    10321 ggcagagaat cgtgagtgac gtagttttta atccggacgg aggcgatgcg tcctcctttg
    10381 gaagccatgc tgaccaccat caggtcgttt tcgatgttgt aaatttggtt ttcgccggta
    10441 agaaaagcag caaactgacc atagttgtcg ttgagtgcct gagcttcggt ttgtccggct
    10501 tctaccagtt cttgttcgta agaggccgtt tgctggctga gcggttgttg aggtgttgct
    10561 tgtattatgc tgtcttgttg gatggcttgt tgcacttttt gctccgtggg tttttggagc
    10621 atgctgaatc cgacaagaat caataaaata agtccaaatc cgattaaagt atttttatcc
    10681 ataatattat ttgttgatgc aggctttaat aaagtctaca aacaaagggt tggggttaag
    10741 tacggtacta ttgtattccg gatggaattg agtgccgaga taccattttt tgtcgctgag
    10801 ctccactatt tccaccaatt gggtgtcggg attgatgccc acgcatttca tgccggcagt
    10861 ttcaaatctt tccttgtagt tgttgttgaa ttcaaagcgg tggcgatgtc tttcctgaat
    10921 atgctcgttt tggtaggcgc gataggcgat ggaattcttg tctacttcgc aatcgaaatt
    10981 acccaggcgc atggtaccta ccatattggc gacgcttttt tgttcatcca tcaggtcgat
    11041 gacattatcg gcagtattgg ggttgaattc ggtggaggtg gcatctttta agtccaatac
    11101 gttacgggcg aattctatgc acatgcattg catacccaga ccaatgccga aagtgggcag
    11161 gttttgttcc cgtgcaaatt ttaaggcaga aattttacct tcgacgcccc ttattccgta
    11221 accggggccg atcaatagac catccagccc cgagagcgtt ttttccacat tgttttcttc
    11281 tattttttcg gccgatatca ggcgcaggtc caggcaatgg tcgctgtaag ccgaggcgtg
    11341 tttcagcgat tccgtaatgg aaatataagc gtcgggcaga ctggtatatt tgcccaccag
    11401 acctatgtga accttttcgc tggcattttt catcttgttg acgaaatcga tccatggctg
    11461 aaggtcggat tctgtattta ccggcagatt gaatttctcc agaataatag tgtccaggtt
    11521 ttcctggcgc actttaagcg gaatttcata aatactgggc aaatcgatgc attctataac
    11581 ggctttttcg tttacgttgc agaacaaggc tactttttta cgtatctctg tagtcagggg
    11641 gtgttcggtg cgcagcacaa gaatgtcggg ttgtaccccc aattcgagca gtaatttgac
    11701 ggaatgttgt acgggtttgg tttttagttc cttggttgcc gacagatagg gaacataagc
    11761 caggtggatg cacatgcaat tgctgctacc caattcccag gttaactgac gcacgctttc
    11821 aataaacggc aaagattcaa tgtcgcctac ggttccgcca atttcggtga tgatgaaatc
    11881 gtattgccct ttgaggctga gttgcctaat ggagcgtttt atttcgtcgg ttatatgagg
    11941 aactacctgc acggttttgc ccatgtaatc gcctcttctt tctttgttga tgactgtctg
    12001 ataaatacgt cccgtagtga tgttgttggc acgggtggtt ttaatgttga ggaaacgctc
    12061 ataatggccc aggtcgagat cggcttcgtg accgtcgcag gttacgtaac actctccatg
    12121 ttccagcgga ttgagggtgc ccgagtcaac attgatgtaa ggatcaaact tttgaatggt
    12181 tactctgtag cctcttgctt gcaggagctt ccccatagct gccgacaagg ctccttttcc
    12241 taaagaagaa actacacctc cggtgacgaa aataaatttg gtatttttca caatcacaag
    12301 tagttatatt aactttacgt tctgcgttac taaaaaaatc taaaagcgca aaagtacgaa
    12361 aaaagtttta aaaagaaagt cttactttcg cagaaaatct caaagtatgc taaacaggat
    12421 aattggtggt tttgtattgc tttttgtttt gcaaatatta ccaatgaatg cccaacaaag
    12481 tatcataccc ggaaattcat tgcattttaa tgctgttccg aattttgata attatctgca
    12541 gccgactccg gaaaaagatt ctttgccgaa agcaccttct accgaaatcg aaattgaata
    12601 ttcgcttgag cgcgccttgc tggacagctt gtcggctatc ctaaggaaaa gccagggact
    12661 ggaatacgac cctgatagtc tttggtttga ctcagacagt ctttggtttg actcagacag
    12721 tctttggttt gactcagata ctcttagatt ttattccgac agtcttggct gggaaaatgc
    12781 actcgacagc cttgggagaa tcttgagcga cacgctgaat cttcagcttt ccgatagttt
    12841 gtcggtcgat accatacccc aagaggagct tgttcccaaa gaacctgaac tatcgccttt
    12901 acaccgttac ataagggact acgatatttt caaagaccgc gtgtatccta agccccccat
    12961 ggaattaccg gaagataccg tgttggatta cccgaagatg agccctttgt ttatgccttt
    13021 ggtctttagc gccagaaaaa gggaatttaa actggaatgg agagaaccca aaaggcaagt
    13081 cattcccgag agttggctcc aaacgcaaga attggggcaa agcatcggaa tcccttatgt
    13141 caagcgcctt gccagacaaa tcctgcttgc cttcgaagtg gaacaaatcg ataagatccg
    13201 ctacctgaat acggatttac cggaacccga aaggctggaa gtgcctctgg aagctaatcg
    13261 ccccatacaa tggagtgcac cccagagagt tcgtccccgg agtattgaag agcttcccac
    13321 cgctcccgta cgttatactc attgggttgt caagggccgt ttatccaacc agttgacaca
    13381 gacgtatgta tcccccaact ggtccagcgg aggtgtgagc aatatgtcta ccttgtcggc
    13441 cttctactgg gtggccaaat acgacaacaa aaaaggtata cagttcgata attttattga
    13501 tatcaaattg ggattcaata ctgtatccaa cgacagtctg cgtaaggtga atatcagcaa
    13561 cgaccagttc caggcttcca gtaaattggg tataagagca atcaagaatt tttactactc
    13621 ggcatcggtc gattttacca ctcaattttt gaacaattac aagcccaata cttacgacat
    13681 gaaagcggcc tttttgtcgc cggctaaact gtatctgggt ttgggtatgg actacaagat
    13741 caaaaacaaa gaaaaaggtt attccttttc tgccttgctg acccctttta cttaccgtct
    13801 gaattacttg caaaacataa ccgctttcaa tcccaaagcc tttggtatag aggaaggaaa
    13861 acatttcggg caggacatgg gtttcaagat tgcgaccacc atcgatatgc aaatcacgga
    13921 atttattagc tggaattcgt atttttattt ctattccgat tttaaataca tagattcaga
    13981 gtggaaaaac accttcaact ttaacgtgaa tcattatctc tcgactcaat tgtttattca
    14041 tatgaaaatg aacaccaaaa acgaaaggcc cgaaggtgaa aaactcatac aattcaaaga
    14101 gttccttagt tttggcctgg tctattattg gtaacatcaa aatataaatt caaatattaa
    14161 ccgacaacct ttgcagattt gttatatctt tgcctcgttt tattacagga cgaagcaaaa
    14221 ggaagatcat ggatgaaatt cgccattacg gattggtgga aagtattgat gaaaaaggcc
    14281 tgcatgttcg aatagaccag tattcggcct gcggagcttg tcaggcgaaa aattattgct
    14341 cttcatccgg gaaaaaagaa aaaatcatcg acatttttaa ttatcagggc cgatacaagg
    14401 tgggagataa ggtcgaaata cagggctcga ctgtcatggg ctacaaagcc gtgctctggg
    14461 cttatgtggt tcctctctgt tttaccgtgt tgatcctggt gttgagtctg accgtcttat
    14521 ttccgggaaa ggaggcgccg gcagccttgc tggccttgtt cagcccgctg gcctattatt
    14581 tggtgttgta ccgttttcgt tctgctttaa aaaaggaatt cagatttaag gttcgatcgt
    14641 tggaagacga cgtaagtatt gagctatgaa tgttatattg atcgctgtta ttgttttggg
    14701 aggtaccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntaa gtattgagct
    14821 atgaatgtta tattgatcgc tgttattgtt ttgggaggta ccggtatcat actggcctct
    14881 gtcatatttt tgacagccaa aaaattctgg gtttacgaag atcccagaat cgatgaagta
    14941 caaaatgttt tgcccggagc taactgcggg ggttgtgctt atcccggttg ccggaatttt
    15001 gccgaagcct gcgtcaaaag cgaaagcctt gacgggcttt tatgtccggt gggaggcaac
    15061 gatgtaatga agtcggtggc ttctcttctg ggcagggatg cagtggaggc cgatcctctg
    15121 gtggctgttt tacgatgcaa cgggacttgt gccaaccggc ccaaaaccag ttgctacgaa
    15181 ggagtttcaa gctgtatggc tgcctccctg ctatacggag gagacactgc ctgcacttat
    15241 ggttgccttg gtatgggcga ttgcgtggcc gtatgccagt ttgatgcttt gtacatagat
    15301 aaggaaaccg gcctgccggt tgtggacgaa gctaaatgta catcttgcgg agcctgcgtc
    15361 agggcctgtc ccagacatct gtttgagtta cgcaaaaaag gtcccaaatc gagacgaatc
    15421 ttcgtttcct gtatgaacaa ggacagaggg gcgctggcgc gtaaagcttg ttccgtggct
    15481 tgtataggtt gtggaaaatg tgaacaggtg tgtaccttcg atgcaatcgc cataaaagat
    15541 aacctggcct acatcgacta caatgcctgt cgcttgtgtc gcaaatgcgt ggaggcttgt
    15601 ccgaccaatt ctatatggga attgaatttt ccgcccagga agaaagcagc cgaacaagaa
    15661 gtttcggcaa caaaagtcga aacaggaaaa gacacaacaa cagtataatt aactatagaa
    15721 taccgggaga aattagagga agactccgaa aatatatgtc aatgagaact ttttcgatgg
    15781 gggggataca tcctcccgaa aataaaatct cggccggaaa ggccatagag caagtgcccc
    15841 tgccggcgca ggtggcaata ttgctgaacc agcattcagg tgcacctgcc gttcccttgg
    15901 ttcaaagggg ggacagagtc aaaacaggac agctgattgc cgaggcggcc ggatatattt
    15961 cggccaacat tcactcttcg gtgacgggta aagtattgaa actcgatgaa gtctacgatg
    16021 cgagcggcta ccgttgtcag gcagttatta tcgaagtgga acctgccgat gaatgggccg
    16081 aggaagtcgt tttgaattcc accctgaaaa gagactgtat actgagcccc gaagagatta
    16141 ttcaaaaaat taaggaagcg ggcgtagtgg gtatgggagg tgccagtttt cccagtcatg
    16201 tcaagttgag ccctcctccg ggtaccaagc ccgaggtgct gatagtgaac gctgcggagt
    16261 gtgaacctta tcttacctcc gaccatcagc taatgttgga aaaaggcatg gagatcattg
    16321 tcggaaccaa gataacaatg atggctctca aggtgaaccg ggcggtaatt gccattgaaa
    16381 acaacaaacc cgacgccatt gccttgctcg aacgattggc tattccctat tctcaaatag
    16441 atgtgatgcc tctggcagtt cattaccccc agggtggaga aaaacaactt attgaagctg
    16501 ttcttggccg tcacgtgcct tcaggagctt taccggtggt taccggcgcc atagtattga
    16561 atgttggcag tatttttgcc aactatgagg cagtgcaaaa aaacaaacct gtcatagacc
    16621 gggtggtctg cgtaacgggc aaaagcgtta agaagccggg caactatctt tgccgtatag
    16681 gaacttccgt acaagatttg attgattttg ccggcggtat gccggattcc acagccaaag
    16741 taatttccgg cggccccatg atgggaaagg ctttgataaa tacgcaagtc cccgtcacca
    16801 aagggacttc gggtattttg ctcatggacg gcaatgaagc ccaccgtaac gaggtataca
    16861 actgcatacg ctgtggagat tgtatcgagg cttgtcctat gggtctggaa ccctatttgt
    16921 tgatgagtct ttcggaattg tccgaatggg aagctatgga aaaagaatgc attatgaatt
    16981 gtattgaatg tggttgttgt tcctatgttt gtccttcgaa ccggccgttg cttgatcatt
    17041 taagggtagg taaattcaag gtgggagcca taatcagagg aagaaaaaac caataagcta
    17101 tggaaaataa actctatgta tctatgtcac cccatattca cggtggtgat agcataagca
    17161 gaaaaatgtg ggatgtcgtc attgccttga tacctgcatt gctggtttcg ctttatttct
    17221 tcgggctggg ttccctgctg gtaacttctg tttcgattgc ttcttgtgtg ctgttcgaat
    17281 acctgattgc acgttttttg ctcaaaaaag aaagcactct gcgcgacggt tcggctatat
    17341 taactggttt gctgctggct ttcaatttgc catccaatct tcctttgtgg atagtggtca
    17401 ttggcgcttt ggtggccatt ggcatggcta agatgtcgtt tggcggcctg ggtaataata
    17461 tctttaaccc ggctttggtg ggcagggtct ttctgttgat ttcttttcct cagcaaatga
    17521 cttcgtggcc cttgcccaaa ccgtttaatc ttcattatct ggatgccgct acgggagcta
    17581 ctcctttgtc ggttatgaaa tcgagtcgtt ttgatcgttt acccgatatg ctggatatgt
    17641 ttatcggcaa ttccggaggc agtctgggcg aagttgctgc ggcagcgctg attctgggag
    17701 gcctcttttt gttgatccga aaagtgatca cctggcatat tccggtatcg atattgctga
    17761 cagtctatat ttttacgggc atactcaggg tggccaatcc cgaagtttat gcagatccgc
    17821 tcagccattt acttacgggt ggcctgatgc tgggagctat ttttatggct accgattacg
    17881 tcacttcacc tatggtaaaa aaaggccagg tgatatatgg gataggtata ggaattatca
    17941 cagtgtgcat aagggttttc ggctcctatc ccgagggagt ctcttttgcc attttgatga
    18001 tgaacggggt aacgcctctg ataaatgcct attgtaaacc taaacgtttt ggggaggtaa
    18061 aaaagaaatg aaaaaactgg aatcttcatt caaaaacatg gtcctggtgc tgggtagtat
    18121 ctctatagtg gctgccgctg ccctgggatg gataaacgaa ctcacttccg agccgatacg
    18181 attggccgcc atagccagac aggagaaggc tattcgcatg gtgtcgccag agtttgacaa
    18241 ccagccggta gaggaacaat tcgagttgat cgattacgaa ggcaacagcc tgatctgttt
    18301 tccggttaaa aaagagggaa aacttctggg ggtggctatt gaaacttttt ctatgaaagg
    18361 tttcaacggt gaagtccggc tgatgttggg tcttaagccc gatggcagta ttaataacta
    18421 tgaggtcttg aagcagagcg aaacgcctgg cctgggtaca caaatgacct attggttcaa
    18481 gaccgacaaa aacaaccaaa gtatactcaa taaagatccg ggaaaggata aagtttgggt
    18541 ggacaaagat ggtggagata ttgatgccat caccgctgcc accattactt ccagagcctt
    18601 tctggatgct gtagaccggg cgtaccgagc ttatatggag catggaggca ccgtggtaga
    18661 tgtagttaca acagcaacta tcgaagaaaa ggaggcgcaa aaatgaagaa taaagttggc
    18721 ataattttga acgggttgat caaagagaac cctacttttg tacttgtatt gggcatgtgc
    18781 ccgaccctgg ccaccaccac ttctgccatt aacggcatag gcatgggcct ggctactgca
    18841 tttgttttga ttttttccaa tatggtgata tcgctcatta aaaacctggt acccgacaac
    18901 gttcgtatac cttcgttcat tgtagtgata gccacttttg taaccattct tgagttggtg
    18961 atgcaggcgt atttacccgc cttgcacgag agtctggggc ttttcattcc cctaatcgta
    19021 gtcaactgta ttgtgcttgg ccgggcggag gccttcgcct ccaaaaactc ggtgcttgat
    19081 tcggcactcg acggtatagg tatgggcttg ggttttactt ttgccctggg tttgctgggt
    19141 atgatacgtg aattgctggg agcaggaacc ttatttggtt tacagatcat ccctgaagat
    19201 tacggcatgc tcatgtttat tctggccccc ggagcctttg tcagtctggc ttatttgata
    19261 gcaatcgtca accgcataag aaaagtatca tgatttatat cactatcttt attgcagccg
    19321 tgtttgtcaa taacatcgtg ctgtcgcagt ttttagggat ttgtcctttt ttgggagtat
    19381 caaaaaagat cagcacggct ttgggaatgt ccggaggtgt ggcttttgtg cttactctgt
    19441 cgactcttat cacctggttg atctttcgct atttgctgga accttttgaa ttggcttttc
    19501 tgcaaacaat cagttttatc ttggttatag cggccttggt tcaattggtc gaaattataa
    19561 taaaaaagat ctctcagcca ctctatcagg ccttgggcgt gtacctgccg cttatttcca
    19621 ccaattgcgc cattctcggt gtagctatcc tcgtaattca aaaggaatac aacttattgg
    19681 aatcggtggt ttttgctttt tcgacggcca taggttttgg gctggctctg gtcttgtttg
    19741 cgggcattcg tgaacaactc agtcgcacca aaatacccaa gggtatgcag ggcacaccga
    19801 tagctttgct ggtggccggt ttgctggcca tggcctttat gggattttcg ggtatagtta
    19861 aattataaat tattaaaata tggcagcatc tattcttgtg acaggcggca caggctacat
    19921 aggttctcat accgtagttg aactgcagca atcgggtcac cgggtaataa tcgtcgataa
    19981 tctttccaat tcaaataaat ctgtgctcgg aggcattgca aagatcaccg gcatcaggcc
    20041 cgattttgaa caggtcgatt gtaccgattt caaagcgatg gatgctttgt ttgccaagta
    20101 cggagacatt aaggccgtca tacattttgc ggccagcaaa gccgtaggcg aatcggtcga
    20161 aaagcctttg ctttattacc gcaacaattt gacatcactg ataaatttgc tggaactgat
    20221 gtctaaatac gggacggaag cgctggtttt ttcctcttcc tgcacagttt atggccagcc
    20281 cgatgtattg cccgtcaacg aagacgctcc cattaagcct gcgctgtcgc cttatggcaa
    20341 taccaaacag atttctgaag agattattac agacagcatc aaagccggag ctaggtacaa
    20401 agccagtatt ttacgctatt tcaatcccat aggcgctcat ccttcggcag aaatcggcga
    20461 gttacccctg ggagtgcccc aaaacctaat tccctatgtt acccagacgg ctgccggtct
    20521 gcgcgagatg ctcagcgtat ttggcaacga ttacaacacg cccgacggat cctgcatcag
    20581 ggattatatc catgtggtcg atttagctaa agcgcatgtc gttgctttgg acaggatgct
    20641 cgacggcaaa tcggccgata agctggaaat attcaacctg ggcacaggca agggactgtc
    20701 ggtgcttgag attgttgaaa cttttcaacg ggtcaccggt gtcaagctca attaccggat
    20761 tgtaggacgt cgtcccggag atattgagca agtttgggcc gatcccgaaa aagccaataa
    20821 agtattgggc tggactgccc gcgagacggt agaagagacc ctcttgtcgg cctggcgttg
    20881 gcaacaaaaa atcagtagct aaccggaggc cttgtaatgt aaagtcgggt atttacaacg
    20941 acagggataa atttaccgta atatagtaga gagcatcgtc gtttttgggt gctaccatcc
    21001 ggaagttggg tgcgagttta acacctttga tcggtgcata ttgaacgccc agtatggcca
    21061 tttgctggtc tccgggattc cagatatcca agggattgtc gtaatctttg ttggaggctg
    21121 catagtccca acggccgaaa agctgaaaat ctttaggcaa tttgatcgta gtgtaggcgg
    21181 agtaaccgtg ttgatttctg tcgtttctga attgcgagtg aaacatacgg agatattcgc
    21241 cgcccagaga gaaagcttgg ttttgatagc cggcaaaaaa ggaggcgatt tgtttacttt
    21301 tttcgtgggt gagattatcc gggtctttgt cgtgcctgtc gtagtaaagc ctgaatatca
    21361 attctttaat gggagtaatg ctgagccccc ctccgtagcg aaaatactta tcggtattga
    21421 gttttttata gccctctccg ttggtaatgg tggcatctgc cgtcagtaga tcgttgatgc
    21481 ggtattgaac gctcagcccc atatcggcac tgtatccgta accgtattga tccagaaaag
    21541 attttaagat atagcgatag ccccagaatt tttcctgaac actgaactgc ttcaaaccga
    21601 taagtccggc atccagaccg aagttccctt ttttccagct aagcatggcg tttttgacgt
    21661 aagcaatgcg ttccagagcc gatccggcca atttactgct gcccatatca aaaaccacct
    21721 tacccgacaa actctcactg aaagcgtatt gataccccag gtaagccctt tccagtccaa
    21781 agccggtttc ttttccggcg gcacctatgc ctgtatggaa atcactgaaa acagtaataa
    21841 tggcttttcc attgggtttg aattcgggat tcgcttgttg cgcctgaata aaaaagaaag
    21901 agcttgctag ccaaagcaat aagaataatc tgattttcat ggttcattta ttgtctaaat
    21961 cctgacagcc gagtgttttt tcaggcagaa tttaaacatc aatcctcctt ccggtctgtt
    22021 tactgctatt atattaccct ggtgaaacaa cacagcatgc ttcactaccg acagtcccag
    22081 tccggtgcct ccggtacggc gatcgcggct ttcttcgagt cgcacaaagc ggtcgaagat
    22141 tttttgaagg tatttttctt tgataccgta acctgtatcg tagtatttaa agtggtaaaa
    22201 ttcagtatct tccttagagc attccacaac aattgagctg tttttgccgg cgtagttgat
    22261 cgagttctca accagatttc taaagatggc gtgcaccagg ttgtagttgc ccgaaatttc
    22321 aagatcggca cctaatctgt tttcgacctt cataccggcc ttgtccagtt gggcctgcag
    22381 ttcgttaatc acatcttcgg caattctacc aatgttcact ttttccctcg gaaagaggtt
    22441 tgaggcttct tcgagcttgg taatcagggc gatatccttg attaatgccg aaagccgcaa
    22501 agtctgggcg taggcacgtt ccacaaagta tttccttttg gtactctcca aatctccatt
    22561 ttcgttcaga atttccagat aaccctgaat gctgctcaca ggggttttga gttcgtgagc
    22621 tatgttgttg gtcatttcct gttttatcaa gcggttacgc tctttttcca gatcggtttg
    22681 ttgtttgctt ttttcgagtt gttcgtagag gcttattatt tttttaccaa taatgcccga
    22741 atcggaatcg ggaaattgaa aacttttata atctaccgta cccttttcga tatccgaagt
    22801 aaaacgcctg agagaatgca ttactcggct gaatttatcg gctttgtaca gcaaaagcgc
    22861 cagcactact ataaagagga ctgccagtcc gtagaaaatc agattttctt gtttcatgac
    22921 atcgcttagt tttacgttat agggcagtgc tagtcgaatg taatgtcctg tattacagcg
    22981 gtgggcatag tagagataat ccgtttgggt cgaaaccgaa cggcggatgg cgtagcccga
    23041 acccttggtc ctcgccgaca gtatctcagg ccggttggca tgattgctca tgtcttcgat
    23101 atggcctatt acattatcaa aaataaccga cccttcacta tcgatgatgg taaagcgcaa
    23161 atcttcgggc agtatgtggg tcacctctat ggtatcgcag ttctgcagca ggtatgcgtc
    23221 tatcatctcg gaataggcag cgagtttgga tcgtatttcc tcggttttgt agcgtttttc
    23281 cctgtcgagc tggaagagaa tggtgataaa cataaaagcc agcgcaatca ggccgaagtt
    23341 aaggtataat ctggttttgt aatgcatgat gtcgggctgt attaaatgtt agattcagga
    23401 aagccagcgg tagccgaaac ccggacgact ttgcaagtat tcgcctgccg cacccatctt
    23461 ttttcgtaat ctggcaatat gtacgtctac ggttctttcc aatacgtaag attcgtctcc
    23521 ccaaacactg tcgaggattt gtccccgcgt gaaataactg tcggccgaat gcatcagcaa
    23581 atccaaaatc tgaaattcct ttttagtcag tgccagcgtt tctccatcca gactggcggt
    23641 ttttgctttg cgattcagga tcagcccccg ggattttaag atttcttcat ctccggaagg
    23701 cttgttgctc tcactgcgac ggaagatggc tttaatgcgt gccaacactt cctgaataga
    23761 aaagggtttg gcaatataat cgtctccacc caggctaaag cccgtcagga gatcgttttc
    23821 ggtgtttttg gcagtcaaaa aaacgatggg cagttgactg ttcatttctg tcctgatttt
    23881 tctggccagt tgatagccgg acatgccacc catcatgaca tcgagaatca gcaaatcata
    23941 gttcaaattc ttgtcaagca ggagttcgag ggcttgttcg gcagagttgg ccgacaccac
    24001 ttcgtaaccg gcgttttcca gattaaactg aagaatttcg caaatatcct gttcgtcgtc
    24061 tacaactaag atgcgtttca tttcgattta tatttatccg tgacgtatat ccctgccttc
    24121 ggagaggaaa ataacagcct cggcgatatt ggtggctccg tctccaatgc gttccattcc
    24181 gtacaagatg cggcccaggt ctagaatatc ggctatccag tagggatgat ctttgtctgt
    24241 tttttcgtgt tcaaaaagtt tcttgtggct caaatgatga agcttatcca attcttcgtc
    24301 gctgttgatt atgctgcggg ccatggtatt atcggcccat aaaaaggcaa agatggcatt
    24361 ttgcaacatg ctgtcggcaa attcaaataa atttacggcg tatttctggt attcggggta
    24421 gatagagtct tgcctgttca ggtattgcat cctccggctg atactgtcca tggtatcggc
    24481 aatgcgttcc agatcgatca ctgtatcgta atatgaaacg atgcgcctga ggtctccggc
    24541 ccggggagta tacaacacga tactattgat tatgtcatca cgaagcttga tctccatatc
    24601 gtcgatagaa tgctcgtttt tctgcatttc atccttcaag gcctgggtgg tatcgcctgc
    24661 cagttgtagt ttaagaatct gcgcctgacg caaaaccaga ttagccatcg ccttgaaatc
    24721 ctgggtcagc cgggccagat aagtttctct tattgtatcc atagtcctgt aattatccga
    24781 attttcctgt caaatattct tctgtgcgct tatccgaggg attggtaaac ataatttcgg
    24841 ttctgtcgta ttccaccagt tctcctaagt acataaacat ggaataatcc gatatgcgcg
    24901 cagcttgtgt catattatgc gttacgatga caatagtaac ttcttttttt aacttgacca
    24961 acaattcttc gatcaggttg gtagcaatag ggtccagagc cgaagtgggc tcgtccataa
    25021 gcaggatatc cggcttcagg gccaacgctc tggctatgca caagcgttgt tgctgtccgc
    25081 ccgaaagaaa agaacctctt ttactcaggc cgtctttaac ttcatcccaa agtcctacgc
    25141 tttttagaga gctttcgact aattcatctt tttcatgacg cgattgcttg atgccgatga
    25201 gtttgtagcc ggccagcaca ttatcgtaaa tgctcatagt ggggaaggga ttcgggcgct
    25261 gaaacaccat accggccatt ctccttactt cgctggggtt gatgtgcagc aaatcccggc
    25321 ctttaagctc tatattaccg ctgatgcgaa tgtcgggata aagttcgtgc atgcggttaa
    25381 tagccctgat cagggtgctt ttaccgcaac ccgacggtcc cattaccgaa gtaatcaagc
    25441 cttcataaat atcggcagat acgttgttga cggcgaattt accgggagta taagatacac
    25501 agagcttttt gagactcagg atgggtgttt tatttaaatt ctccattttt ttgcaagttg
    25561 tttggccaga atatttaaac tcaaaaccag caagagtaaa aacagggaag cactccaaat
    25621 catgctttga aggttgggat cgttgtaaaa ttcccatatc agcaatgaaa ctgccgaagc
    25681 gggcttagtg atgtcccatt gtatggttgt tgctcccaaa gccgtcagca tcagcggagc
    25741 ggtttcgcct ataactctgg acacagccag caggataccg gtaaagacgc ctccgaaagc
    25801 cgaaggcaaa agcactttca acactaccga tctgtacgga gaacccagag ccagtccggc
    25861 ttctttgagg ctggccggca aaaggttcat ggtctcttcg gtagaacgga caatcagagg
    25921 taacatcata atagccaaag cgacactccc cgctaaagcc gaatagccct taaggggaat
    25981 aaccacccaa agataagcaa ttatacccaa aataatagag ggagtaccct gcaataagtc
    26041 ggtcaggaaa cgaaccacgg tcgagaattt ggtttttcgg ttctcggaaa gatagatgcc
    26101 gcacatcacc cccaggggaa tggcaataac cgaagctaca agcaccatca tcaaggtgcc
    26161 cacaataccg ttggcaatac cgccgggaat gggctggttg ttgttgatgg ccagcaaggc
    26221 atccagggta gtgggggtgg tttttgtgaa aaaatcccaa ttgatttgtc tccagccctt
    26281 gagaaagact tttcctataa tcagaaccag tggtactgcc gtgagggcag aaaatattac
    26341 caccgacagc gtcagtaact tatttttgaa taatctgtat tttaatgaac tcatacactg
    26401 tgtcgtttaa tcatcaacct gcccaaaaaa ttaacgatgg ccgttatcaa aaatagtagc
    26461 aggccgatag ctataagcga agacaattta agtccgtcgg cttcgccaaa ttgattggct
    26521 ataatgctgg ccatggtgtt gcctgtgtcc attaaaccgc ggggaattct gctggtgtta
    26581 cctataagca tggttacggc catggtttcg ccgatggctc tccccaaggc caaaacatag
    26641 gccgaaaaga ttcccgaaaa actggttgga aagataatgt ttcgtatgac ttcggcccgg
    26701 gtagctccca ggctgtaggc tccttctttc aactcctggg gaaccatacg aataaattcg
    26761 gcgctcagcg aagctgcata aggaataatc atgatagcca gcaccagcga tgaggtcaat
    26821 acgccaaaac cctgttccga taggcccagg gactggatca aaggcctcag ggcataaaag
    26881 ccccataagc cgtacactat cgaaggaatg ccggccagca gatcgactat gccgcccaaa
    26941 acacccgcca ctttttttcc cctgaagtat tctcctacaa aaaaagctac aggcaaggaa
    27001 aagggtaagc aaattaacag ggccagcaca gaggtcatta aggtccccgc aatgaaagcc
    27061 agggcaccgt attgttcagt gctctggccc gttccggaaa tccacttatc cgaaaaaata
    27121 aagccaaaaa atccgaattc tctgaaggca ggaaccgctc ctttgctcaa agaaaagaac
    27181 atacctgcaa agatcagcaa gaggatcaaa gctgagacca ggagcaagga tctgaagatt
    27241 ttgtccgaca tcagcagccc aaattacaga gacttaccgt tataagtaat ctccgacaag
    27301 gccttttgtg ctttgtcaat gacctctttc gggagcggag catagtgtac catggtagtt
    27361 tgagcttgtg ctgccgggct aagtataaag ttcagcaggc tgagcgtggc ccgggcctga
    27421 tcttcgctcc tattggcgta agcctgatct ttgtacacaa ttatccacgt taggcaactg
    27481 ataggatagg agccttttgc gctggaattg gttatcatgc tgcgagtatc gtcggcgatt
    27541 ccctcctgag ctgcggccga gatagaaagg ctcgaaggca aaataaattc tccggctgcg
    27601 ttttgcatat aagcgatggg gctgttaagc gcaaaggcat attcagaacc tatgtaacct
    27661 atggcgcctt cggtctggct gatggtgccg gccactccgg gattaccttt ggcggctatg
    27721 ccatgggcaa aattgagcga tttgcctgtg ccgagagtat tttcccagtc tttgtttatt
    27781 ttggtcatat agtcgctgaa cacaaaagtg gtaccgctgc cgtcggagcg atatacagga
    27841 gtaatcgctt tatcgggtaa acttactgca ggattgattg cttggatggc ggcatcgttc
    27901 cagcgagtga ttttacccaa gtaaattccg gcgatgatat ctccggtcag attcagggaa
    27961 cttacatccg gcaggttgta cgacaataca acggctccca gacaggtggg aacgtgaatg
    28021 gtggctttca tctcggccat ctcctcatcg ctgagataag cgtcgctgcc tgcaaaatcg
    28081 accacttcgt ctttaaggct gcgtatgccc ccgccacttc ctatgcctcc gtaagagaca
    28141 tcgatgcctg cgttttcggt aaaagcttta aagactaaat tgtaataggg cagggggaaa
    28201 gtggctcctg ctccggacaa actggttttc tgactacaac tgatcaacag taaactgctg
    28261 acagccaaaa ggaaaattgc ttttttcatt ttcttcgtta ttgattaaat tgaaaggttt
    28321 aatttttacg cgaaattcaa caaagcatat gtcagacctg tttcaaactt gttacgatcc
    28381 tgttaagatg ttaagtttcg ctcgggttca atcgcgtgaa tataccagac cattttggag
    28441 tttatattgt ataggtccgt aattcttagc cagatagatt tcttttaccg tgtgatcttc
    28501 ccattcctct gccagttcaa ggcagcaaaa atgcattact cccctataaa tgctgtttcc
    28561 tacccggagg ctgtccagca ggctgagtgt agaaatggtt tggtattcac agggccggca
    28621 cctgctcatc aacaacagga aaaccaacag cagcnnnnnn nnnnnnnnnn nnnnnnnnnn
    28681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28741 nnnnnnnnnn nnnnggccag gctttcgatt actatattgt gccggtgact gttgaaagga
    28801 ggcagttcta ccgcatattc taccggtttc ttggtgttgg gggtgattaa taagattctt
    28861 ccattgatgt agagtctgct ggctgcggtg gtgttcagat acagagagta ggcttctccg
    28921 taccgggttt cgaggcagcc cgaccagcgc aggcttttta cgttggtatt aaggggacta
    28981 ccggggaggc ccgaggcgga atcgatgcag tttgaaaaac gattgtccga ataacgataa
    29041 accctaagcc cgctgccggt agccggtttt ggattgtcca acaaatagtc tataatactc
    29101 aattcgttgt ttcgggccag gtagacaaaa gacaacagac catcgtcgat agcgttccga
    29161 tcgatttgcg gctctgagca aaaacgtccg gcatcgaggt tggaaaccaa ttcccaatca
    29221 ctgtaggcgg ctgctgaggt agcagcgtag atttcagctc cgttggcaac gacataggca
    29281 ttgtcgaaag catccaggat aatttgaacc cggcaatagg aattgacttt ttggcctttg
    29341 ttgtagatgt aattgtgatg ccagttgccg tcggtgtcgc ggaagcggtg gtgcaatacg
    29401 gctttgctgc gtgaactgct ccagttggag tcggtgcccg agtccatata tgagttaact
    29461 atatgtaccc tgcctttgct gtcgcaggat tgtgtttcct gatttatgta gcctttgttg
    29521 tggggaatat cggctatttt gagtgtcgcg atgccctgcc tcaggcaatt gcccggactg
    29581 cgactgtcgg tataattgat ggcttcactc gtggctaccc ggtttccgta ggtgtctttc
    29641 caactgcggc cgtggtcgtc gctgtaagcg tagcaaaggt cgtggttggt ttgtccccca
    29701 aaatcgtctc tccagcacca ggacacatgc aggcggcctt gtttgtcgta atccatgcgg
    29761 ttgatgtagg cgcaggtgtt aggattggtg tccatgccct ggatgtagcg gcctacaagg
    29821 ctccagttgt gggtgtcgcc gtcgtattcg cgcaagtagc tgtcgccgtc tccgctcagt
    29881 ttgtatcggc actcaaaaag caggttgccg tcgggttttt cgataaaacg gggataggtt
    29941 acatcgggta cgggaacgcc ggcttcgagt tcgtcggtga aaggcccgaa attcccggcc
    30001 gtccaggcaa gcttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaggta
    30121 cggttttatt ttattttgag aaatacggct ttttgattta ctttgtacac aaacacctga
    30181 gataccatgt ccgcccgcct tactggatta tttgtttttg caatactgct tgtttttccc
    30241 ttgcggggag aaatgcaaat ttcatttgaa tactttcctt atatggacca attgccttcc
    30301 agttcggtga gaagagtatt gcaggacagc gaaggttata tgtggtttgg tactctcgac
    30361 ggggtctgtc gttacgacgg ctatcaggta agaactttcc ggtctgatat gaataatcct
    30421 tcgctgctga ccaacaacga catccaaagc ctggccgaag ataaacaaaa ccgcatttgg
    30481 ataggcacta agcaaggaat caatcttttg gaccgcaaca ccctgaacat tactcccttc
    30541 gacgaatccc ccgtacaggg agataacatc aatgcactcg cctgcgacga taaaggcaat
    30601 atgtggattg ctaccccgcg gggtttgttt tgctacgaca acacccgcaa ctgtttaaaa
    30661 acttactgga gcgatcccta cgacgaaaaa aacctgcccg atcagaccat aagtcatgtt
    30721 tcaaaagaca agcagggcaa cgtctggatt atgttctgga acgggggctt atgccgttac
    30781 ctgcccgaaa gcgacaattt tgaacgtttt ccgcccacga aagaaatgag cggtcctttc
    30841 aggctttatc aggacaaaaa cgatcagtac tggctctgta cctggggcag cggtttcttc
    30901 aagttcgatc ccgaagcccc gcccgaaaac aggtataccc gcattcccgt gtacaacacg
    30961 gcaggggagg aggaaaacat cttgttcagt atggtgcagg acgatcgcta cggttatttt
    31021 tggctgatgt ccctaaccgg tttgtatgtg tttgagtacg acgaacaaaa taccaggttg
    31081 attcaggtag atatctccga acatttgatc ggatccagca agctgtacag cgagataatc
    31141 aaagacaagg ataacaattt gtggataggc gcctacagcg aaggagtcat ttttgtgaat
    31201 ttcgaccttc ctaatgtgcg taattacggt atggaagtgc ttcgtcaaaa gctgggtttc
    31261 cccgtaagta tcagggctat gtcggaagac tccgatggtt tggtctggtt gggggtaaac
    31321 cgttacggtg tgttttttta cgatcgccac gaaaacaggg tattcagcat tgatgaaata
    31381 cccaccattg aaaatcatca ggaattgagc aacctgaaaa cagtaaacta tatccgcgag
    31441 ataaaaagtc gtggggagca ttggatttgt tgtgacgacc agtggatata tatatttaga
    31501 aaagacgggc gaggcgtata ctatgccgga cgtctcgaca tagacgttag taatgaattg
    31561 aactgggcct cgggaaaagt gctttttgaa gacaaagact cttgcgtctg ggtggggatg
    31621 ctcaatcagg gattggtcaa aataagtccc gaaaaccggg ttaccatggt ggaacccata
    31681 cctatggtaa caggtataac tcaagacctg cacggagaca tttgggtaag cagcgaaaaa
    31741 gtgggtctct gttgtctgag acccttcgga gagacttacc ggctaattcg ttacgataag
    31801 tattccgaag gattgaatac caacaacata caatctgtct gtgcagatct ttccggtaaa
    31861 ctctggcttg gaaccaaaga gggccgtctg ctcacatttg acaagaacac cggcagtttc
    31921 gaagacatga gtccggcctg cgccatgacg ggtgaaagcg ttctgaattt gttggtcgac
    31981 cggaacaata atttgtggat atctaccaat aaagaagtga ccggtttcaa tcacaggacc
    32041 ttgacctcca cttcgtacag catttccgac aacctggttg tcaagtcttt tatcaaaggt
    32101 tcttattctg ccaccagcac cgaggaggtg ttgtttggag gcaacaaagg cttttgcgcc
    32161 ttttcgccag ggaagcagca caacgacagc ctcaaaagca aaagcgtccg ggtacatatt
    32221 accgatatta aagtacacaa ccagtctgta ttaagcaaca acgatgagca cgactacaat
    32281 cccgaactca aaaggctgat tttgggacct gcggaacgta atgtggaatt tgaattttcc
    32341 agtctgaatt atgccgcacc gtttaaggtg caatacgcct ataaattaga agggctcgac
    32401 tcggaatgga ccaaagtgaa cgctacccgg cgctttgctt tgtacaataa tttacatcag
    32461 ggcaaatata agctcatgtt tatggcaacc gacgaaaacg gccgctggag tgagccgctt
    32521 cccggcctgg aaatcataaa gaggcccccc ttgtatctga gttggtgggc aatcctgctg
    32581 tatgttgttt tgctgatttt gttgttctat gccttaattc gtttttttac caatagagta
    32641 cgtttgaatc agcaacttaa tattgctcaa attgaaaaag ccaaatccga agagctcact
    32701 cagaccaagc tcaggtatct gaccaatgta tcgcacgaac tattgactcc tctgacggtg
    32761 atttcctgtc tggtcgaaga tgtggatcag gtgtcgaaag gcgaatactg gcagcacgaa
    32821 gtgatgaaag tcaatttaag ccggctcaaa agattgttac agcaaatact tgatttcaga
    32881 aaagtagaaa gcggctccat gaagcttaag gtggcggaat cgaacatgat cgaattcgtg
    32941 gaacgcattt gtgcttttaa ttttgttcct ctggccaaag aaagagacat ccgtttcgaa
    33001 ttgcagcttc ccgattatcc tgttcaaggc tggtatgatc ccgaaaaact ggatattatc
    33061 ttgttcaacc tgctttcgaa tgcttttaag ttcacgcccg aagggggatc cgtacgcctg
    33121 agcctctctc tgctgcaaaa aagtaaaggt cgtgttgctc agctgatagt cagcgacacc
    33181 ggtcggggca tacaacccga cgagttggag caaatattca ctcgttttta ttcgaacgat
    33241 tcaagtctta cggtcgaaaa ccacggtata gggctgactc tgaccaaaga acttgtcgaa
    33301 atgcatcacg gcagcatcag ggtcgaaagc gaacccggca agggaagcac ctttatcctg
    33361 gagtttccca tcgaaaaatc ggcctatgcg cctgaagaac tggttgctga aaccaccgaa
    33421 gaagaagccg ttctttccga tcaggccagc gataccggta aatcgcaagc ttcaactaaa
    33481 cataaggaag acgtgcatat cctgatagtg gaagacaatg cgcaactttg cgacaccatc
    33541 gaggccattt tgaacagaaa ttaccatacc tacaaggcgt tcaacggtaa agaagcagtt
    33601 gcccttatca acagcaggca tattgatttg gtagtcagtg atattattat gcccgggatg
    33661 gacggcctgg aattatgccg tactattaaa aacaatttgg ataccagtca tatcgaagtg
    33721 ttgttgctga cggccaaaaa ttccatcaac gaccgcattg agagttacaa cgcgggtgca
    33781 gacggatatt tgtccaaacc ctttgaatta aaagtcttgc aagccaaaat caacagcttg
    33841 attcgcaacc gcaaacgcaa gatggattct ttcaaacata atgccgagat caacatttcc
    33901 tcgctcgaat atacttcgct cgacgaaaaa ttccttgaaa atgccgtgtc cattgttgaa
    33961 gaacacattg ccgtaaccga ttttaacctc gatcttctgt cgagtcgact gaatatgtcc
    34021 aagtcgtcgc tttaccgcaa aattaagtcg ctcaccggcc tctcgcctgt agaatttacc
    34081 aaaaacatcc gtctgaaaca cgcctgccag atgctgaccg accgggccgg aaatatttcc
    34141 gatattgcct atgccgttgg ctttgccgac cctaagtatt tcacttcttg ctttaaagcc
    34201 gagtttggtt taacccccac tgagtacata aaaaagatca aaaaagaagc ccggtaaaaa
    34261 aggacttaat ttttgcggct tagtcatatt ggcagaataa tcaccattaa tgaacaattt
    34321 ttggtattat aagctgttga ttgcttctac ttttgtcaat caagagtctt tagttatatg
    34381 acaacgtccg aattttttac gagaaggttt ttgcgcccag tcctgttttt ctcttttaca
    34441 gtgctgctgc tttcgtgcag ggctaaggat cgggactgca ttcgtcccgg agaaatctgg
    34501 cccgacaata acggagtgca tatcaatgcc cacgggggcg gtatgctgta ttaccgggga
    34561 caatattatt ggtttggcga agacaagagc gagcgcagca acgctgctct caagggcgtt
    34621 cgttgttatt cctcgaaaga tttgtgtcac tggaaagacg aaggtattgt gcttgctgtt
    34681 gtagaagacg agcccgctca tgaattgacc aaaggctgta tcattgaacg ccccaaggtg
    34741 gtttacaaca aaaagaccgg cacttttgta atgtggtttc accatgaatt gcgttggcga
    34801 ggctacagcg cggcgcgggt gggtttggcc gtgagcgaca aggtgacggg accttaccgt
    34861 tttatacatt cctacaggcc taatccgggc atctggcccg aaaacatgag cgaggaagat
    34921 aagtcgctcg atatggagga agcctcccgc cttgaatcct ggactcccga atggttggag
    34981 gccgtcagga aaggcttttt tgtaatccgc gatttcgaag gaggacagat gtcgagggac
    35041 atgaccgttt ttgttgacga tgacagcaaa gcctatcata tctattcttc cgaagaaaac
    35101 ctgactctac atattgccga actcagtgac gattatcaga gccatacagg caaatacata
    35161 cgtatcatac cggcagggca caatgaagct cctgccattt tcaaaaaaga tggacgctat
    35221 tttatgatca cttccggttg caccggctgg aggcccaacg ctgcccgcct gctgacggcc
    35281 acttccattt ggggaccctg ggagcaaaag cccaatccct ggcaaggtga acaggcagat
    35341 atttctttcg attcacaaag tacttttata ttcccgctgg ccggcaaaaa agacgctttt
    35401 gtctttatgg ccgatcgctg gagaccccgg aatccatcgg acggacgtta tatttggttg
    35461 ccgcttgaat ttgaagacgg gctaccggta ttacgctgga gagacagttg gtctcctgct
    35521 aaagctttta aaaaatgaat agagttatga aaaaatcctt gttgatttat ttgttgattt
    35581 taggagggtt cagcttgtat gggcgaggca ccgcccccta tgtcctgcag aatgtgtacg
    35641 cccgcaatta cgagactctg aacggatcgt ggaatcatat agtggatcct ttcgacaacg
    35701 gctactacga ttatcgcatg aacatcaacc ggagcggttt ctttcgtaac caaaaacagg
    35761 ccaatccttc ggatctggta gaatacaatt tcgatacatc gccttgccta caagtacccg
    35821 gcgactggaa tacccaggac gatcgcctgt ttttttacga aggcagcgtt tggtataaac
    35881 gagatttcaa ctttgagccc cgggagggag aaaggcaatt tatctatttt ggtgccgtca
    35941 attatttttg caatatatat ctgaacggta gggaaatagc cactcacgaa ggaggcttta
    36001 cccctttcag cgtcgaagtg acagacaagc ttagaaaagg acataatttc ctgattctaa
    36061 gagtaaacaa cgatcgtcgt cccgaaaatg tgcccaccgt caatttcgac tggtggaatt
    36121 acggaggcat cacccgagac gtacttttgg taagtttgcc taaacttttt gtggacgatt
    36181 attcgttgag gttgaaaaaa ggaagctaca gggagctgga aggctatata tctttgagcg
    36241 atcctcaggc aggagtggaa ctgagcctcg aaattcccga actgaaaatc aaacaaacca
    36301 ttcaaaccgg agagcagggc agggcagact tcaatatcaa ggccaaaccc gaattgtgga
    36361 cgccccaaaa tcctaaactc taccggatta tcttgcgtca gggcaacgaa gttatcgaag
    36421 acaacatcgg attcagacac atcaccaccg aaggcaagaa aatattgctc aacggccaac
    36481 aagtcttttt gagcggtatc tccatacacg aggaagcccc ttaccgtcag ggcagggttt
    36541 attccaaaga agaatcggtt ttgctcctgt cgtgggctaa agaaatgggc tgtaattttg
    36601 tgcgtctggc ccattatccg cacaacgaac atatggtgag agctgccgaa gaaatgggtc
    36661 tgatggtatg gtcggaaatc cctgtttatt ggaccatagc ttgggacaac ccggatacct
    36721 accaaaatgc ctccaatcag ctccgggata tgatggagcg cgataaaaac cgctgcgccg
    36781 tggtggtttg gtcggtggcc aacgaaactc ctcacagcga agctcgggat aaattcctga
    36841 taggcttggc taatcaggtg cgcgaaaagg acgacagccg cttgttgagc atggctatgg
    36901 aagtcaccgg ccagggcaac aacatcagcc gggtacgcga caacatgagc gattatgtgg
    36961 atatcataag tgtaaatact tatctcggat ggtatggcgg ctccttggat gccattttaa
    37021 gccgcacctg ggaaatacct tacgacaaac cggtgttttt cagtgaatac ggtgcgggtg
    37081 ccttgcaagg tttgcatggt gaagaaaacg ttcgctggac cgaagagtac caggagcggt
    37141 tgtataaaaa caccattgaa atgtttgata aagtggaggg attttccggt atgtcccctt
    37201 ggatattaat ggatttccgc tctacccggc gccagttaag aggcgttcag gatttcttca
    37261 atcgtaaggg agtgatctct gaacgaggaa tcaggaagaa ggctttttac gtgttgcaaa
    37321 actactataa aagcaaggat tattaagggt taggactgat gccggtcttc tgtgttgaac
    37381 gccggcagac tatcaaggaa ggctgttcaa tcgggcagcc ttctttatta gtattgagct
    37441 gttaaagaat tccccgtggg taatcgacag ctcctactgt tctcccagtg tttgttgttc
    37501 caaaaaccta attattttga cctctttcag accggatgcc atcaaatcat ctcccggctt
    37561 cataatttga ggatatgaat aggttatacg ttgctcttcg ggtaaagttg catcataaac
    37621 catgcggttt atcaacgaat tggctacgtg gatctcaagc gtattttcac cctcctttaa
    37681 atagtccgat atttcgagct gccagggtga gcaccagacg atgccggctt cgttggagtt
    37741 gacaaatact ttggctacaa aatggggatt tcctaaatca ataaaagtcc ttttgtccga
    37801 ttgttcagcg taaaaattcc ttttgtagac tgctgttccg gaataatatt ttattcccgc
    37861 ttcattattt ttggtccaat catgcaaagt atcaaacacc acttccccgg gtcctcccaa
    37921 accgggatca aaaaacacgt tccaattatc gtttaataat gttgtatcct tatctgattc
    37981 agtccaaaca ataggaggta ggtcttcata gatatcggtt attacaacaa aaaacgactc
    38041 acgggcagcc atatgcaaag gaatggtaag cgaatttcca ttgtgattgc taacaggtat
    38101 cgaaaacctt ttacctgtaa cgctattcca aagttgtgct tgtttgcctg atgccgaaaa
    38161 gctaaataga ttttcttcag gctcatcttt atgattatct aaaaagtaga tttcagcgtt
    38221 ggaaagactt cgatgtgtaa aatatatttt attggacttt gtgtttccgg tttccattga
    38281 aatatcgggt ttaatttttg cctgatttaa tgcttccgac aaaggcatac cccaataaac
    38341 agttcccttt ccgaccgtgt tagatccttc attatttggg tttattcccc aaagttcgtc
    38401 tgccagtttt tgatatgccg gtctcatttc acggtcccgg tgtgattctg aaaaagttgg
    38461 tttgtcaccg taaactttaa tgcctttttt taccatttct gcaatcttct ccagggcctt
    38521 atagggtatg ttgctgtttc gcggcaatat catcattttg taattgacac caccgggtaa
    38581 gtttatcagg ccattctgta ctgacatgcg cgtatgtaag gcatgtgacg taaaggcgtc
    38641 aaaatcgaag ccaccgggaa tgtccggcag acggtaagtc aatattttga cgggtgcgtt
    38701 ttctccaagg taaacacaaa ggtctatgga tggtttgccc aggcgtaaaa tacctgcatt
    38761 tcttgcctga taattccaga aaggttcact atagtcccac catgtgttgt tgcgattaat
    38821 ggcgtattgt ctgccaccgc ccgtgctgcc cggtatttta tcgagccagg gctgatacgg
    38881 cgatgcgcaa atcacaaatt cgtttattcc aaaggcataa gcataatcgg cgtaggattt
    38941 taaatcggat aaagagtggt aatatttagc atcggtaaaa gcttcggcag atgcaatggg
    39001 cttgttgtac aaatgggctg ccgacgaact ttctttaata tcgtagttcc catcaggatg
    39061 aataggccag aactcgcctt gtggttttga aactttactt ttagcttgaa tgggatctcc
    39121 cacaatgcac agggcattgc ctatcgcctg tgctgtaaaa atcaatccat tttcattaca
    39181 caaacgttca aaactgccgt aataattatc cgaaatcatg tcggcaatat ttcttctgac
    39241 atcgaagaga aatccgttgg aggtttctac atcgtttatc acataaccca tcatggcagg
    39301 aagatatgga gtcggatcgt atccctgacg ggaggtgaat tcctggataa aattgtccgt
    39361 ccaattttga gcacccgcct cgtggctatc catggctaca cccgataaaa taccactatt
    39421 tgaagatttc agtgagtcaa ttatcacctg aacataatgc ttccaatggt attcggctgc
    39481 ttcaaccgaa agcttatcgc actcacgacc catcaggttt ttacggccat gtttggtgct
    39541 gccgccggtt ggaacataag caaagcgcat caccatccat ctgcccgccg ggacttccca
    39601 tttcaaagta ccatcgggca acatcttatc ggtgagattg atgattttag aaagatctat
    39661 agcctcgctt gccggataat cgggtgtttt gtctttttca atatactctg aaaagaaacc
    39721 tgctttttct tcccattggt caactttggc ggcagcatgt aaaacaagat ttccaatctg
    39781 catatcgtcg ggtgtttttt catctttttc ccaccaatca tggagattca gtctgtaata
    39841 ttttccttct acagccgcga aagaaatggt tttttgtcgc cagctctcgt gtgcccggta
    39901 aacaggtttc aggtcacaaa cttttgagta gttaattccg tcttgtgaaa cttccagttg
    39961 acctaaatcg ggtagaatgc gatagcctgt acctacaaaa gtttcctgtg gcggccccgg
    40021 aatgttggtt gcactggttg tggctttgcc ttttggcctc acctggtagg taatacttcg
    40081 tgcagtgaac ggctcatcaa attccagatt tatgtaaacg ggcttaccat caggggaaaa
    40141 cggaattttg cttagtctat ccgactttgg atcgaacaaa ttctttaaat caatttctgc
    40201 cttgttcgat gttaacttaa caggtcggtt agacgatgaa atgccgacac cgtttggtgc
    40261 cggaaaagcc aaaacagcca catccttata gaaattgtat ttgttcaatg gagcttcgag
    40321 cacaccttca aaatacctac ctccatcaat catcagttca gtagaggcaa gtcgcttcat
    40381 agcgtattca ttgcttatcc aggaacctcc ggcaacatag ccgtttgaaa tatgtatctc
    40441 aaacgaaaga ccgattcgtt cggcttccct ggctgcaaaa cagagcattt cccaccactt
    40501 atcggaaaag ccgtccaaag catcgggagc ttttccgtgt gactggtcgt aataaactac
    40561 accggctata cccgcttttt tgaacgcttc caaatcggct gtaattccct ctttcgttgt
    40621 ttcggtctct ccgtgaaacc accatgtttt ggtgcgtgtg ctgtcgggaa cagttgcaaa
    40681 ataatctacg gcatcggcta aactatgtct gccgaatttt tgatttggat gtgagcatcc
    40741 aaaaagaatt attgtaaata gtattgaaaa aagtctgcaa tgcatctgtt tgtgagatgg
    40801 ttgtgattta atgctgtttt gttcattcat catgaagtac aattctgctt gtttttatgg
    40861 aacgaaaaca ggcttcttac cagttcccgg aaaaaggacc ttttgattag cgtcttgctt
    40921 gattcccttc catatataat tccggcggtc aaataactga taagtagctc ctattccatc
    40981 gagcccccga cgatatccaa caactcgctg gtgatggctt gctggcggag cttgttgtat
    41041 tgtaatttga tttcttcgag taattcatcg gcattgtcgg tggcggtttg catggccagc
    41101 atacgggctg tatgctctga tacgctcgaa tccaacaatg ctaaatacag gcgcatattg
    41161 atgatgtagg gcaccaaatc gtgcaataaa ttttccctgt cgggctcgat gataaagtcg
    41221 tcttcataaa tacgctctct gacgacgggt ttttctatta catccctttc tttcaaatcc
    41281 agaggaagca gtgtgcgtgc ctccaaaacc tgcgagctct tgcttttggc gtggtgatag
    41341 atgagttcaa ttttatcaac ttttccgctt agatacaatt gtttcagttg attggctaaa
    41401 tcgagcacga ctttcgcatt gggtttgtcg aggatgaagt ccaaattttc aaaaccggag
    41461 gggatgttgt atcccatttt cacgcttgcc tgggctatct ttttcccgat gggaataacc
    41521 aaaatgtt
//
LOCUS       DAOSTD010000005        33973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1420_length_33973_cov_13.394835, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000005 DAOSTD010000000
VERSION     DAOSTD010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33973
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1420_length_33973_cov_13.394835"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="PLI37_00660"
     CDS             complement(<1..1130)
                     /locus_tag="PLI37_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOQ57560.1"
                     /translation="MAAVDLLKVKQRFGIIGNAAGLNRAIDVAVQVAATDLSVLITGE
                     SGVGKESFPQILHQYSHRKHGPYIAVNCGAIPEGTMDSELFGHEKGSFTGALAERKGY
                     FEVADGGTIFLDEVGDLPIPTQARLLRVLESGEFIRVGSSKTQKTNVRIVAATNIDVP
                     RAVQTGRFREDLYYRLNTVPIKIPPLRERGEDVVRLFRKFAADFSEKYRMPPIQLQED
                     AQQLLQAYRWPGNIRQLKNITEQISIIERQRNISADILEKYLPENSFEKTPAIYRGHQ
                     TSSQAGFFNSEREILYQVLFDMKKDMRDLKVLVHDLMQNAGMEEGLIRSKDLIYPTYI
                     DKTTELISKRDDSSSGKNVRGQDMPQIQDAAEYVEESLSLDEL"
     gene            complement(1130..2221)
                     /gene="pdxA"
                     /locus_tag="PLI37_00665"
     CDS             complement(1130..2221)
                     /gene="pdxA"
                     /locus_tag="PLI37_00665"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOQ57561.1"
                     /translation="MEDNKLIIGITQGDINSISYEVILKTLLESKVYEPNAVVIYGSA
                     KVAAYHKKALDINQLSINVINSVEEAEWGAVNLINCTDDDVKVEFGKSTPIAGKASFQ
                     ALEAATVDMKAGLIDVLVTGPINKKNIQSSTFQFPGHTEYLEQVFGNGDKALMLMVSD
                     VLRVAVATGHIALSEVPSALSVPLLVEKIKLLDQSLKKDFGVIKPRIAVLALNPHAGE
                     EGLLGREEIEIIAPAIQKAQDQGCYCFGPIAADGFFGADYFDRYDAILAMYHDQGLIP
                     FKALCRDEGVNFTAGLPIVRTSPAHGTAYALAGNNEASESSFRSALYLACDVYRNRVR
                     YFENNKNPLKKQHIVHSGDLSDLPVQDEE"
     gene            complement(2231..3274)
                     /locus_tag="PLI37_00670"
     CDS             complement(2231..3274)
                     /locus_tag="PLI37_00670"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOQ57562.1"
                     /translation="MNKYALISAKVSRFLLLTALILFVSCKDKTIRNPLAPTAVGTPW
                     EVLVVLEKDLWDSPAGESIFSLLREDVPGLPQPESRFEISRCDDDEFTGILKPVRNII
                     QIEISDKYSEPKLFFYRDQWASNQYVLRIVAPSEELFVFFVERNAEIIISYFELAERE
                     RSLQYIRKTHNRAFSEKVYEQFGFKILVPGFMKLSKQADQFFWASNYQNKKRQDMVIY
                     TYPYTDKNTFTPEYLNAKRDSVMKINIPGGPEGSYMSTQEIDPPIFRGINVKGKFAAE
                     IRGLWEVKGDMMGGPFVSLTRLDEVNQRVVTVEVFVYAPEEDKRNLLRHNEAALYSLE
                     LPGEFELEIEEQK"
     gene            complement(3402..4424)
                     /gene="rlmN"
                     /locus_tag="PLI37_00675"
     CDS             complement(3402..4424)
                     /gene="rlmN"
                     /locus_tag="PLI37_00675"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOQ57563.1"
                     /translation="MDKYILLGKSFEELKTWTLAQGLPSYTASQLFEWIYRKPAKSFD
                     EMTNISLQNRALLKEKSELGIYPPIQKSTSKDGTIKYLFPSREGYGVEAVYIPESHRA
                     TLCVSSQAGCKMNCIFCMTGRMGFKAQLSAGEILNQILSLPQGASLSNIVFMGMGEPA
                     DNIDAVIQSLNLLTSPRAMAWSPRRITVSSIGVLPGLKRLLDETRCHIAISLHSTDAA
                     QRLAWMPAQKTWPLLSTIELLKTYDFSKQRRLSFEYIMFKNLNDRIEDAKALLRMLNG
                     LRCRLNLIRFHAFPESELRGSDEEQIQSFATYLNNKGLQTTIRQSRGEDVQAACGMLS
                     SQYKSG"
     gene            complement(4524..6665)
                     /locus_tag="PLI37_00680"
     CDS             complement(4524..6665)
                     /locus_tag="PLI37_00680"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOQ57564.1"
                     /translation="MATLQKIRSQAVLLLIVVGVALFAFIIGDFLTSGSTILRQSQEN
                     IAEINGSPLNYKEYDARILEMEEVYKMQTGQNNLQSDIMHQIRESVFETIVRERLLDD
                     QAQDLGVSVTAKELYDMVNGQNVHYMVQQMPFFQNPETGRFDRNMMMNFLRTIQQEDF
                     SMYSAEAQEQIKQMKNYWLFWENNLKYVRLDEKMVNMVTKAVGANSLDAKDEFEASRR
                     SVDFEYASKSYASVPDSSFKVSLKELSRRYEEQKERFKQEPYRSAAYVVVDILPSQED
                     NDAIKARIDKIATDFSTEENVATTVNSESDLPYVDCYIANNQFSGSLKDFVQQAAVGE
                     VKGPYFEDNAWIMLRMMGKINRSDSVKARQIFISNAGDRKAAEQLADSLLNVLRKGED
                     FAALAGEYSQDRTANDGGEMGWFREIDAYSGIGPEFAEACFTAYKNQFLTVNSKFGWH
                     VVQIQDKTKAVAKSKVAQLYLSVTPSSKTYSAEYNKLSKLMAGNSKAEDFMLAAREAG
                     YQVSNAAVVKSTDNTIGNVPEMRQAVRFVFNNKLGDVSTVLENSNNQFVVVAVTGIYD
                     NKYLSLDQVRDELSREIINEKKAASIIASLQVDDNTVLSDLAKSHSMQTDTVRLVNFS
                     MRRITGLGEEPALLAAVMSAAKGELSQPVAGKNAVYVFRVLSQVEPEIEFDVVAEKRN
                     QDMNNNYRLTYQAYQALRDAAKVEDNRIRFY"
     gene            complement(6811..7407)
                     /gene="lptC"
                     /locus_tag="PLI37_00685"
     CDS             complement(6811..7407)
                     /gene="lptC"
                     /locus_tag="PLI37_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOQ57565.1"
                     /translation="MLLRSLFGKYTGITVASFVTVILFLSFSCKREKADILPNFNSRD
                     SVPGMRADSIVTLISDSGRIRYKVITASWLVYDQLPDPYWFFPEKIYFERFDDSLRIE
                     SIVKSDTARYYTNRKLWELKKNVQVMNLAGEKFETPLLYWDQNLHKIYSDSFIRIEQK
                     DWISTGIGFESNESMTKYSILNSQGNFPIDPEEENSDQ"
     gene            complement(7410..8705)
                     /locus_tag="PLI37_00690"
     CDS             complement(7410..8705)
                     /locus_tag="PLI37_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57566.1"
                     /translation="MAQKGVEDGSMYGHGEDSIRCLQNLSLYQPYAKQGDYASALEFW
                     EIVYAECPASRVGIYIDGAKIKAWQWNKESDPAKKTQIFDELMAVYDQRIKYFGDHDR
                     YPTPWILGKKALDYLQFNPNGDMRLPYEWLKTAIYDSPTANFEPAYATAFISVSRNLL
                     SDDAHKENFFNDYIKAVERLDAKLAVTSPGPEKDRIVGIKNGVDVLFSNSGAADCETL
                     TALFAERIEQNKDNQNFLNSVISLLKRAKCTDTDTYFLASEYRHAVNPTAESAEGCAY
                     QAIKKEDYKKAAEYLKEAIELETNPDGKADYAFLAASALFAGKSYEQARSMALQAAEY
                     RENFGAPYVLIAKMYAGSVDYFDDPILKRAVYWAAVDKLQKAKAVDPSVSDEVDELIA
                     RYKEQYPDTEDIFMHPDLTVGKMFRIGGWIQENTLVRNK"
     gene            complement(8762..9979)
                     /locus_tag="PLI37_00695"
     CDS             complement(8762..9979)
                     /locus_tag="PLI37_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57567.1"
                     /translation="MMFLFYWSVSAQNNTFSPYSRYGYGIISEPAFGGASGMGGIGYG
                     LRSSGQINPMNPASYSAVDSLSFLFDFGLSATYTHFRENNLREARLNSNIEYAAVKIP
                     LSKDWGLSLGLYEYTRLGYAFSSSGSLSDLDGNTLIYSNAYSASGGINNAYLGTSVLF
                     FKHLSLGVNINYKFGSLINKSVLSYPYNAEINPTSVSNVLVVNHFNIDAGLQYEQWFG
                     YKHRLVLGVNYTPDGLMDVNYTTTTTTLDTLIQEHPGLSFGFPQNLGLGFSYTYDNRL
                     TLGMDFQHQAWNKTSFFGVSDSLSLRTRLALGAEFLPLNIAQRYYQAIKYRMGLYYSD
                     SYIKFAPGNLKEIGLSVGLGLPLRNQRTALNLAMEYGKILTPLPGMVQEHYWRVKLSL
                     AFSETWFVKRRFN"
     gene            complement(10017..10790)
                     /locus_tag="PLI37_00700"
     CDS             complement(10017..10790)
                     /locus_tag="PLI37_00700"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOQ57568.1"
                     /translation="MNLIIDQGNSVCKWAFFENVPKAKTDYTRLDALEPAAVFHTRHP
                     EEMLSREIFKQFKPRAAIYSSVLNHTDDVKYEAIKQNVSLCFRFDSTTPIPVKNNYSQ
                     PQSLGADRLAAAVGAWTLAPGKPSLIVDLGTAITYDLLTAEGVYEGGNIAPGIYLRMK
                     ALHENTKALPLPEISVGDFPEFGKDTPSAIKAGVLQGIIHEINGYILQHRQKHPELLT
                     FLTGGDLIYFAGKLKSDIFARPNLVLTGLHRILIDYVNS"
     gene            complement(10806..>11335)
                     /locus_tag="PLI37_00705"
     CDS             complement(10806..>11335)
                     /locus_tag="PLI37_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOQ57569.1"
                     /translation="DRSKVLLIDFGKFDKSGYAYVCQGFDTTLYDCLSSGSPLFLKYQ
                     KLNFVFPLNSEHPRGGIRFFHRFCEENNLGSQLIERHIRKEDIRKGEAFLLVSHTDMI
                     EAVKICRAKKLTLGKDVGLVTFNDAPMLEVIGNGITCISVDFREMGLLAARFINHNER
                     LQTYVPTRLIVRGSL"
     assembly_gap    11336..11435
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11436..11662)
                     /locus_tag="PLI37_00710"
     CDS             complement(<11436..11662)
                     /locus_tag="PLI37_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOQ57570.1"
                     /translation="MRVEFQSNTTKVKQLADALRQAIAQGEYKEGEMLPSINKLSAEL
                     NLARDTVFKAFQELKLSGAIQSAPTKGYYVSS"
     gene            complement(11784..>13607)
                     /locus_tag="PLI37_00715"
     CDS             complement(11784..>13607)
                     /locus_tag="PLI37_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ57571.1"
                     /translation="KVRNNLSLEAGEKKTINQLIEVKQPQLWSPSSPYLYRIHSQVRQ
                     GKIMLDGGISRIGIRLAEFKGKEGFWLNGKPYGQLIGTNRHQDFAYVGNAMPNSQHWR
                     DVKKLREAGCEIIRAAHYPQDPAFMDACDELGIFMIVATPGWQFWNRDPQFQEYVLDD
                     VTQMIRRDRNHPSVLLWEPILNETRFPLDFSMKALEITQKEFPYPGAPLAAADMHSEG
                     VAQHYGVVYGWPKDEGAAEQCIFTREFGENVDDWYAQNNNNRASRSWGELPQLVQALS
                     LADSYDEMFRTSGQFIGGAQWHAFDHQRGYHPDPYWGGQTDCFRQPKYAYYMFKSQVS
                     PQLEHPLIETGPMVFIAHEISPFSNADLVVFSNCDSVRLICREQDTIVKPVLHKDKGM
                     PNAPVIFENVFDFWQMRELSYLQKNWQQVSFVAEGIIDGKTVCSTKKMPSRRSTKLRL
                     RIDHDGQQLIADGSDFLVVIAEVTDDNGNVRRLAKDNILFSVEGEGEIIGDASIGANP
                     RAVEFGSAPVLIRSTRQAGKIKVKAGVLFEGQHSPAPAEIEFESIPARLPFNYLESYQ
                     SSNQEDYRFDKGKDRVKLSEQEIKTLLKEVEQQQKDFGVEK"
     assembly_gap    13608..13707
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13708..14244)
                     /locus_tag="PLI37_00720"
     CDS             complement(<13708..14244)
                     /locus_tag="PLI37_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HOQ57572.1"
                     /translation="MRKLVLFIALFLSLTSLQAYQPEISIAGFFPLEGSGRSVYNFNP
                     GWRFYRGDVPEAQNPDFDDQAWPIVNLPHTVQLMPAEGSGGRNYQGKAWYRKRFVPDS
                     SLQGQQISLYFEAVMGRSTVYLNGKQVLEHLGGYLPFAVNLNEFGVEAGDTCLIAVCA
                     DNSDDKSYPPGKTQYTLDF"
     gene            complement(14268..16583)
                     /locus_tag="PLI37_00725"
     CDS             complement(14268..16583)
                     /locus_tag="PLI37_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 domain-containing protein"
                     /protein_id="HOQ57573.1"
                     /translation="MKNSKHLFLSLVLLSAWTLGFAKPADLLKDYELTWTTQSKGSNE
                     SMPCGGGDIGMNVWVEQGELLVYFSRSGSFDENSCFLKGGRLRLSFPDEPLDTVEFLQ
                     CLKLSRGLVNIQAGSKGNKVDIDVWADVFKPVIHLDIRSASPRKAVISYENWRYRDRL
                     LRKNESQSNSYKWAPPADLKTSADKIDINENGIVFYHQNPSSGTMFDVVVQQQGMEEV
                     KEQMTDPLSDLRFGGELCADGFVFKDTITGVYAGTDFKAWRLESRKPSKNHAIRLVFH
                     TTQQVDSWHKGLDSIKGLIKPRSDKQLNADWWATFWDRSFICIDPEQPDTESWKVGRN
                     YQLFRYMLACNLRGEAPTKFNGGLFTFDPVHVREDRPFTPDFRNWGGGTFTAQNQRLV
                     YFGMLKSGDFDLMIPQFEFYRKSLKNAELRSNHYWGHNGACYTEQMEHFGLPNPSEYG
                     WKRPENYDPGLEYNAWLEYQWETVLEFCLMILESHRYAGSDIGPYMEFIESTLTFFDE
                     HYRFLARQRGSKELNADGQLILYPTSACETYKMAYNSTTCIAGLKVVLESLLALENLS
                     SDQRQRWENMLQTIPPISFREIEGKKVIAPALHWERINNTEVPQLYPVFPWRIYNVVS
                     EDKDTAINTYWLDPDVREFYSHVGWKQYNIFAACLGLTEEAKKLTLLKFKDAEHRFPC
                     FWGPGFDWTPDHNWGGSASIGLQEMLLQTEGERILLFPAWPAGWDVHFKLHAPGQTTV
                     EAELKDGKLLRLEVSPKERLADVESRLVIQE"
     gene            complement(16573..18036)
                     /locus_tag="PLI37_00730"
     CDS             complement(16573..18036)
                     /locus_tag="PLI37_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006281868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOQ57574.1"
                     /translation="MKYFVHLLLLLFVLVLPEKMEAKLRLPAFISSNMVLQQQQETKI
                     WGWSESGNSVKLKASWDNKQYATKANSEGYWELKFNTPEAGGPYTILLDDGEELLLEN
                     VLIGELWFCSGQSNMEMPMKGYPSQGIEGGLQDLMHSKDDQLRLFTVKRAALASRAND
                     VQGHWQEASPGTVADFSATAYYFGRYLRELLGVPVGLINSSLGGSFIEAWMSAESLEG
                     LSDLPIPRTQEEVRSSTHTATALYNGMVHPFLGMNIKGCIWYQGESNTGRPESYKDLL
                     TQMVSTWRKDWGIGDFPFYYCQIAPYDYALITPKGEQAVNAALLREAQAQAEDLIPNC
                     GMAVLMDAGLISDIHPRKKRQAGERLALLALVGSYGLESPEHRSPRYSACEQRDSMLI
                     LSFDRAGLGLDRTRGSLKHFWIAGQDKVFYPAQAKINRDKIQLWSASVPKPVAARYAF
                     DNISEGDLFGVNGQAVSSFRTDNWSRDEIKFFEKHEK"
     gene            complement(18038..>18515)
                     /locus_tag="PLI37_00735"
     CDS             complement(18038..>18515)
                     /locus_tag="PLI37_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57575.1"
                     /translation="KSMESTDLFDWRKSDDMDIKYYAGTAEYHSAFTLDKIPEAEKIY
                     LKFDSVNVMAEVFLNGKSCGIVWTQPYLLEVSSALKTGKNELKINVANTWYNRVQAIN
                     HKLIQEDDYWTNARIWRVNRPRSREGFSLQNFPVEYLQESGIQGDIKLVWTINQAQ"
     assembly_gap    18516..18615
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18616..21295)
                     /locus_tag="PLI37_00740"
     CDS             complement(<18616..21295)
                     /locus_tag="PLI37_00740"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOQ57576.1"
                     /translation="MKRTAIVLLSFLLLGTFSCRRAASLDEVFLNPPDAAKPWIFWYW
                     INGAVTKEGITADLEAMKEIGLEGAYLMPIRDSSRVQYMSNTVLQLSDEWWEMVRFSM
                     EEAGRLGLKMGMHICDGFALAGGPWITPEMSMQKVVYSETLIKGGRIEQLKLDKPETE
                     IDYYRDIAVFALPAPALESSENLRPKVSSSDRQELSFLLDGSGRFKAKDDCWIQYTFD
                     QAFTARSLTIVTGGNNFQAHRLRVAVSNDGKNFCDVKQLVPARRGWQDTDADATYTLP
                     ETTARYYRFYWSPEGTEPAAEDIDPAKWKPELSITKIRLNSRPLIENFEGKSGVVWRL
                     SQRTTENNLAAANCVQLGDIINISDKMDESGNLSNLDLPQGDWLILRMGHTSTGHENE
                     TGGGGRGLECDKFNPEAVRLQFDNWFGAVYKHVDKSLLKGVLTNMHSDSWECGSQNWT
                     PNFLEEFRQRRSYDLLPYLPLYAGIPIGSADISESVLLDIRQTVTELVNDVFFATMSE
                     LAAQKNCVYSSECVSPTMLSDGMLHYQRVDLPMGEYWLDSPTHDKPNDMADAISGAHI
                     YGKNLVQAEGFTQLRGEWNEHPRMVKTLGDFNFAQGVNKLFFHVYCHHPFIGKYPGMT
                     LDGIGLFMQRGQTWWPYASEWVNYLSRCQAMLQRGVPVNDIAVFTGEELPRRAILPDR
                     LVASLPGLFGEEALASEQLRKLNQGAPVHSTSVNVTASANMYTANQWIDPLRGYKYDS
                     FNKDAFLRLARAENGRMVLPGGAGYRVLVFPEPHQMAPDAGYMSLEVARKIKELQDAG
                     VIVLLPSERPVCIPDYHQKEQRNRELEQLTHAIWEKAQQQACLLPFKEENMNRFGLEA
                     DVLFKDLQGKELYDLAWNHRREGASDIWFISNQLDSI"
     gene            complement(21399..>22943)
                     /locus_tag="PLI37_00745"
     CDS             complement(21399..>22943)
                     /locus_tag="PLI37_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ57577.1"
                     /translation="RWERLLSIARNTADDRPVMTSEYVHCMGNALGNFKEYWDEIYSH
                     PRMLGGFIWDWVDQALAKPLEDGRVQMSYGGDFGDYPNSKAFCMNGVILAERQTTPKY
                     WEVKKVYQPALFEQGKDEYHYRITNRNHHTNLDQYNLSWQVTSDGKTIETGNMDLPAV
                     APGDSHELKLPLTFGKKYRGDVRLFLSLQLKEDALWAQKGHELAWEEFNLKKAALTAP
                     ELKKGKIGIDDKEQILIVEGKDFSQQWDKQTGALVSLVFKRQEMLSREEALFPQPNFQ
                     AYRAPTDNDAGFGNWLAKDWSTHLMATPQRKLLNFSCNPEPDGPVEIKTESLYTYLKG
                     TIRCTSVFRIGADGSMDVEHQFHPEGELPVLPRLGVAWAFAEDLQQLQWYGPGPLETY
                     PDRKTGMRTGLWSSTVADQLFPYPRPQESGNHEETQYLILKNSRNKGLKITAVEDVFS
                     FSALPYLATDLSNCRHYTDLQARKAVICSIDAQQLGLGNSSCGPGVLKKYTVKKTQVR
                     YRIEGL"
     assembly_gap    22944..23043
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23044..24236)
                     /locus_tag="PLI37_00750"
     CDS             complement(<23044..24236)
                     /locus_tag="PLI37_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ57578.1"
                     /translation="MKKNIGLLIAGLIIGSCLCSAQAFRPLWENHRVLEINRLPARAS
                     YVPYLEEKQDRTMSLNGNWKFQYVPKVEEAPTSFYRNDYDDAGWAEIPVPSNWELHGY
                     GTPIYISAGYSFKIEPPLVSSEPDPRYTSFSERTPTGSYRRTFDLPAAWSGKKIFLRF
                     DGVMSAFNVWVNEQYVGYSQGSMEMSEFDVTGCVKPGKNLIAVQVFRYSDGSYLEDQD
                     MWRLSGIYRDVSLYATEKIRIYDFGVRTELDDTYTNARLLIEPKLSVYDGQTADGYVL
                     RADLYDAEGKAVFEQTLEQDAVPVLNREIRSNILNARTPQRGARSFGWFDVEVASPHL
                     WTAETPYLYTLELGLYNKGGQAVEKIQTKVGFRSVEIKQGQVLINGKPIRFRGVNRHE
                     HDPFTG"
     gene            complement(24247..26313)
                     /locus_tag="PLI37_00755"
     CDS             complement(24247..26313)
                     /locus_tag="PLI37_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOQ57579.1"
                     /translation="MKKTIMLCALLACTGLYAQDKGLVNTTKSPYARIWNTDVSAVSW
                     TDGFWGERFEVCRDTMVINMWHVMSDPEVSHAFRNLEIAAGEAEGRHSGPPFNDGDFF
                     KWFESAAAVYAVTKDPKLDALMDRIITTIAKAQREDGYMHTQVIIPERKRQREQASQQ
                     SGPVGTAVGTAQGGAFSDRLNFETYNHGHLMTAACVHYRATGKRNMLELAIKAADFLC
                     RFYETATAELARNAICPSHYMGVVEMYRTTGDPKYLELSKNLINIRGMVENGTDDNQD
                     RVPFRQQKLAMGHAVRANYLYAGVADVYAETGEALLLENLESIWSDIVYRKMYITGGC
                     GALYDGTSPDGTNYTPDSIQKVHQSYGRAYQLPNSTAHNETCANIGNMFFNWRMFLLG
                     GDAKYVDVLERTLYNSVLSGISLGGTKYFYTNPLRLSKEFPYVLRWPKERTEYISCFC
                     CPPNTVRTICEAQNYAYSLSKEGVWLNLYGSSNLATKLADGSDIEIVQRSDYPWDGKV
                     ELTFAKLPRKADFSLFFRIPGWCDHYELSINGNPVELQVEKGYARVQAKWKKTDNITF
                     NMRIQPRLIESNPLLEETRNQTAVMWGPLVYCLESADLGNYKIDNISIPSDISFQPEM
                     MEIDGSRLMSLKGEAWYVDQQNWDNVLYREVSSAKKKVPVRLIPYYAFGNRGFEEMTV
                     WMPLDR"
     gene            complement(26326..29106)
                     /locus_tag="PLI37_00760"
     CDS             complement(26326..29106)
                     /locus_tag="PLI37_00760"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOQ57580.1"
                     /translation="MKRLILISALVSLVFLSCKQDQSSAPDLKVVELSCEMFKDPIGI
                     DAPNPRLSWKLQTEARGVRQTAYRLMVASSEEKLAAGQADVWDSGKQNSEASILIPYA
                     GPELRSSRYYYWKVQIWTNANKEAVESETAGWATGLFTENDWKNSRWIGLDGPMPWDV
                     EKAHARLSARYYRNEFNCAKPVRQALVHISGLGLYELWINGGRIGNHVMTPAPTDYRR
                     SILYNSYDVTSSLQQGQNALGVIVGNGRYYAMRQNYKPYKWANFGYPKLRMILNIEYE
                     DGTRQTIGSNERWMMTADGPIISNNEFDGEEYDARKEFPGWTEVGFKTGRNWFPAQRV
                     TIPTAVIRAQPNPEMKISDVFHPQSITRINKNTYILDMGQNMTGWIRMKVKGKAGQEV
                     KLRFAEILQEDGNLGMANLRDALVTDKYILKGDPQGEEWAPIFTTHGFRYVELSNYPG
                     EPKPENFIGEFVSDEMEDLGYFESSSEVLNGIVRNAYWGIKGNYKGMPIDCPQRNERM
                     PWLGDRTIGAYGESFLFENDKLYAKWMDDIREAQRWDGSIPDVAPAYWNYYSDDVTWP
                     AAFFFISEMLYRQFGDLRPIEKNYESMKLFMEHMRRHYLKDGLLVADKYGDWCMPPEA
                     PEIIHSKDPSRITEGALISTAYFYKLYHIMSGFANLLNKPEDATVYLIMAQDMKTAFN
                     RHFYNSEKQYYSNNTVTANLLPLAFDLVPAEARKGVEENVQKTLLETYGGTMATGVIG
                     GSWLMRELTAMGRGDIAFLLASTTKQPSWGYMLTKGATTIWELWNGDTAAPSMNSGNH
                     VMLLGDLLIWCFENLAGISSDTEQVAYKRIIMKPDFGIADLNFINASYKTPYGLVVSN
                     WKKSEGYLDWTIKVPANASALVYLPAVQKKNIKESDRPIGSSRTIRYLGTENNCTLIE
                     LGSGEYHFNVQL"
     gene            complement(29112..30554)
                     /locus_tag="PLI37_00765"
     CDS             complement(29112..30554)
                     /locus_tag="PLI37_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HOQ57581.1"
                     /translation="MKLKNLILKNSLLVLTLVLLACGQKEELPWDNGKLQVDATQCYL
                     QHENGKPFFWLGETGWLLPERLNRLEAEFYLENCRQAGYNVVQVQTINGVPAVNTYGQ
                     LSMIDGFDFSKINQEGVYGYWDHMDYIVETAESKGIYIGMVCIWGGLVKRGLMDEEQA
                     RAYGTFLAERYKDRPNIIWFIGGDIRGDVKPEVWHALAESIKAIDKNHLMTFHPFGRT
                     LSAIWMHNAEWLDFNMFQSGHRRYEQSEPGKEFLPDSTEEDNWRYVEISKTYEPLKPV
                     IDGEPSYEGIPQGLHDPKEPLWQGKDVRRYAYWSVFAGSFGHTYGNSSIMQMYKGGPR
                     PGAYGATVHWVEALKDEGFNQMKYLKNLILSFPFFERIPDQSVIAGENGVRYERLSAT
                     RGNDYLLVYNYTSRPMEIDLTKISGQTKSVWYMDPTSGALSYLGEFENGVQLFQSPKP
                     QAEGNDIVLIAVDAAKKYVDKSWTVLPDLK"
     gene            complement(30567..>31532)
                     /locus_tag="PLI37_00770"
     CDS             complement(30567..>31532)
                     /locus_tag="PLI37_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOQ57582.1"
                     /translation="SVILPFLYYWRYGDDRLIREYYPVMKKYVDYLESRSDNHIVMHG
                     LGDWYDYGDFKSGFSRNTPVPLVGTAHLYLDIQLLVRAATMLGIREDIDYYTQLGNEV
                     NKAFHERFFRADSCIYGTGSQTSHALPLFLDMVEPPYRQRVLDRLVADIEAHGNRLTT
                     GEVGNRYMFQVLARNGLNELMYKMHNHEETPGYGFQVKFGATTLTEQWDPRQGASWNH
                     FMLGSIDEWFFRSLGGIQFDHEQAPGAQHLIIKPEVVGDLTRVKCRTQNLYGTIGVEW
                     EIDEAKQFTMAVEIPANCDAKVFLPGEDMPELIQSGKHQFIKQLP"
     assembly_gap    31533..31632
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31633..32769)
                     /locus_tag="PLI37_00775"
     CDS             complement(<31633..32769)
                     /locus_tag="PLI37_00775"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HOQ57583.1"
                     /translation="MKNRQSKSIDFVKTKSFISRFQLLLGLLLLAGSLSSCVPEIRIG
                     KMSLDYQEGPLTLQNKDFRLGWQILASGNSVVQEAYELEFYRWEASQKSLLWSSGKVT
                     ENNSQQVLYEGPELQARQKYAWRVRIWDQQGRTTAWSKQQEFWTKPEEAWLDAQWIGA
                     ISRADSRLPEGRQFEGNLVNRDPESKALWAATDPLSKQSLYLRRALNLDKPIEEALVY
                     ISGLGHYELSLNGQKVGDSEFAPLWTDYDKTVYYNVYDVKDLLKKGENVAGVLLGNGF
                     FNVQGGRYRKLLVSFGPPTLFFKLFVRYTDGSTGEWISDQNWKYDFSPITFNCIYGGE
                     DYDANLEQTGWDKPGFDDSNWRAVVVQEAPKGVLLAQTANPVKIM"
     gene            33045..>33973
                     /locus_tag="PLI37_00780"
     CDS             33045..>33973
                     /locus_tag="PLI37_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOQ57584.1"
                     /translation="MIESKIEEGLKEMRAFYRTNTTKDYRYRIHSLKTLSSSIAKHEN
                     ELMETLATDLHKSHFESFATEISIVQQEIRFQIRHLRKWSRTRRLATPMVFFPSRSYL
                     YKEPYGVVLIIAPWNYPFHLLFNPLVGALAAGNCVMLKTSPLAPATAGVMEKIVSCFP
                     GDYISIWHGHREVNQALLKARFDYIFYTGSPAVGKVVMEAASKHLTPVTLELGGKSPC
                     IVDKDANIRFAARRIAWGKTINAGQTCIAPDYLLVHHSVKEEFLLEYKQAIFSMFGSD
                     PQASPDYPRIVSSSALARLSGYLKEGKIVLGGA"
BASE COUNT         8645 a         8210 c         7372 g         9246 t
ORIGIN      
        1 agttcatcca gcgaaagcga ttcctccaca tactcggctg catcctgaat ctgaggcatg
       61 tcttggccac gcacattttt accgcttgag ctatcgtcac gtttgctgat cagttcagtt
      121 gttttatcta tgtaggtggg ataaatcagg tctttgctgc gtatcaatcc ttcttccatg
      181 ccggcatttt gcatcaaatc gtgaaccaac actttcaaat ctctcatgtc ctttttcata
      241 tcgaataaga cctggtacaa aatttctctt tcgctgttga aaaagccggc ttgagaagag
      301 gtttgatggc ctctgtaaat agccggggtt ttctcgaaag aattctcggg caggtatttc
      361 tctaaaatat cggccgaaat attgcgttgc cgttcaataa ttgaaatttg ctcggttatg
      421 tttttcagct gacgaatatt tcccggccac cgataggcct gtaaaagttg ttgtgcatct
      481 tcctggagtt gtatgggagg catacggtat ttttctgaaa aatcagctgc aaattttcta
      541 aatagtcgaa cgacatcttc gccccgttca cgcaagggcg gaatcttgat aggtacagta
      601 tttaaacggt aatataaatc ttctctgaag cgaccggttt gcactgcccg gggaacatca
      661 atgttggtgg cagccacaat tcttacatta gttttttgag ttttagaaga acccacacgt
      721 atgaactcac ccgattccaa tactctgagc agacgggcct gggtgggaat aggcaaatct
      781 cccacttcat ctaaaaaaat ggtgcctccg tccgccactt caaaataacc tttgcgttcg
      841 gccagcgccc cggtaaaaga gcctttttca tggccaaaca attccgaatc catggtgcct
      901 tcgggtatgg ctccgcagtt gacggcaatg taggggccgt gtttacgatg actgtattga
      961 tgcaaaatct gaggaaaact ctctttgccc accccgctct cgccggtaat taatacggaa
     1021 agatcggtgg cagccacttg aacagctaca tctatagccc ggttcaaacc ggccgcgttc
     1081 cctatgatac cgaaacgctg cttaactttt aataaatcga ctgctgccat tattcttcat
     1141 cttgaacagg taaatcggac aaatcgcccg aatggacaat atgttgtttt ttaagtggat
     1201 ttttgttgtt ttcgaaataa cgaacacgat tcctgtaaac atcacaggcc aggtacaaag
     1261 ccgatctgaa agaactttcg gaagcctcat tgttgcctgc cagcgcgtaa gcggtgccat
     1321 gtgccggcga agtacgtact ataggtaaac cggcggtaaa attaacccct tcgtcccggc
     1381 ataaggcttt aaatggaatg agcccctgat cgtggtacat ggccaggata gcatcgtagc
     1441 ggtcaaaata atcagccccg aaaaaaccgt cggcagctat cggtccaaaa caatagcagc
     1501 cttgatcctg ggctttttgt atagcggggg cgataatctc aatctcttcc ctacccaaca
     1561 agccttcttc gccggcatgc ggattcaggg caagtacggc aatgcggggc ttgatcaccc
     1621 caaaatcttt tttgagcgac tgatccaata acttgatttt ttccaccaag agcggaaccg
     1681 acaaagccga aggtacttcg gacaaagcga tatgaccggt agccactgcc acacgcaaga
     1741 catcgctcac cataagcatc aaagccttgt ctccattacc aaacacctgt tcgaggtatt
     1801 ccgtatgacc gggaaactga aatgtagagg attgaatgtt ctttttattg atggggcccg
     1861 ttaccagtac atcgatcaag ccggccttca tatcgaccgt agcggcttcc agggcttgaa
     1921 aagaggcctt gccggctata ggggtggatt taccgaattc caccttcaca tcgtcgtctg
     1981 tgcaattgat aagattgacg gctccccatt cggcttcctc gacagagttg atcacattaa
     2041 tacttaactg gttaatatcg agtgcttttt tatgataggc cgccaccttg gccgagccgt
     2101 aaatcactac ggcattcggt tcatacactt ttgactccaa caaggtcttg agaataactt
     2161 cgtaggatat gctgtttatg tcaccttgag taatacctat tattaattta ttgtcttcca
     2221 tttagttctg ttatttttgt tcttctattt ccaattcaaa ttctcccggg agttccaatg
     2281 aatacagggc ggcttcgttg tgtcgtaata ggtttcgttt atcctcttcg ggagcatata
     2341 caaagacctc taccgtcact actctttggt ttacttcgtc aagacgggtc aggcttacaa
     2401 aaggacctcc catcatatcg ccttttactt cccataaacc gcgtatttcg gcggcgaatt
     2461 ttccttttac gtttatgcct ctaaatatcg gaggatctat ctcctgagta ctcatatagg
     2521 aaccctcggg acctccgggt atatttattt tcatcaccga atctcttttg gcgttcaggt
     2581 attcgggggt aaaggtgttt ttgtcggtgt aaggataggt ataaattacc atgtcctgcc
     2641 gttttttgtt ttggtaattc gaggcccaaa aaaattggtc agcctgcttg cttaacttca
     2701 taaaacccgg aaccaatatt ttaaacccaa attgttcata cactttctca gaaaatgcgc
     2761 gattgtgcgt tttacgaatg tattgcaagg aacgctccct ttcggctaat tcaaaataag
     2821 aaataattat ctctgcattt ctttcaacaa aaaagacaaa caattcctca ctgggtgcca
     2881 caattctcaa cacatattgg ttactggccc attggtcgcg gtaaaaaaac aatttaggtt
     2941 ccgaatactt atcggagatt tcaatctgaa taatattacg taccggtttt aaaatgccgg
     3001 taaactcatc gtcgtcgcaa cgggaaatct caaaacgcga ctcaggctgg ggtaaacccg
     3061 gaacgtcttc tctgagcaaa gaaaaaatgc tttcacctgc cggactgtcc caaaggtcct
     3121 tttccaagac cacaagcacc tcccaggggg taccaactgc cgtgggagcc aaaggattac
     3181 gtatcgtttt atccttacac gatacgaaaa gaatcagggc cgttaaaagc aaaaaccggc
     3241 ttactttagc ggaaataagg gcgtatttgt tcatacctaa tggatttaat gcaacaaatg
     3301 acaaaaatag catactttta cggtatatcc gtagtttttc ctgatttttt aaccaagaat
     3361 ctgacttttt gtcatcaaag aggtggaact cagattgcga gttagccaga cttatactgg
     3421 ctggacaaca tgccgcaggc agcttgcacg tcttctcccc gggattgcct tattgtagtt
     3481 tgtaaaccct tattattgag gtatgttgca aacgattgta tctgttcttc gtccgatcct
     3541 ctgagttcgc tttcgggaaa agcatgaaaa cgaattaaat taagtcggca cctgagtccg
     3601 ttcaacatac gaagcagggc tttggcatct tcgatgcgat cgttgagatt tttgaacata
     3661 atatattcaa aggatagacg gcgttgttta ctgaaatcgt aagtcttgag caattctatg
     3721 gtcgaaagta agggccaggt tttttgggcc ggcatccagg ccaatcgttg agcggcatcg
     3781 gtcgaatgca gacttatagc aatatggcat ctggtttcgt ccaacagacg ctttaaacct
     3841 ggcagtactc cgatgctcga aaccgtaatc cgtcttgggc tccaggccat tgcccggggt
     3901 gaagtcaaca gattcaggct ttgaataact gcatcgatat tatctgcagg ttctcccata
     3961 cccataaaaa caatattact caaagaagca ccctggggca gggataaaat ttgattgagg
     4021 atttcgcccg ccgatagctg agccttgaag cccatgcgtc ctgtcataca aaaaatgcaa
     4081 ttcattttgc atccggcttg cgacgaaaca cagagcgtgg cacgatggct ttccggaatg
     4141 taaaccgcct ctacaccgta gccttccctg cttggaaaaa gatatttaat tgttccgtct
     4201 ttcgaagtgc ttttctgtat gggcgggtat atgccaagct cagacttttc ttttagcaga
     4261 gctctgtttt gaagcgagat attagtcatc tcatcaaaac tcttagccgg tttacggtag
     4321 atccactcaa acaattgaga agcagtatac gaaggcagcc cctgagcaag agtccaggtt
     4381 tttaattctt cgaatgactt gcctaacaat atgtacttat ccattgccgt aaaaaaagcg
     4441 gtgcctcata agtacccaac tgctccgttt ggatacttct aagacacctg ctgtttttca
     4501 attgaatagt tgttttgaga gaatcaataa aaacgtattc gattgtcttc gacttttgcg
     4561 gcatcacgca gggcttggta agcctgatag gtcaatcgat agttgttgtt catatcctga
     4621 tttctttttt ctgcaaccac gtcaaactca atctcaggtt caacctgact gagcaccctg
     4681 aatacataga ccgcattttt accggcaacc ggttgggaca attcaccttt ggcagcgctc
     4741 attacagcag ctaacagagc gggttcttcg cccaatccgg taattcttcg catggaaaag
     4801 tttaccaaac gcaccgtgtc ggtttgcatg gagtgggatt tggccaaatc agacaaaaca
     4861 gtattatcgt ctacttgcag agaagcaatg atagaggcgg ctttcttttc attaatgatt
     4921 tcacggctta gctcatcgcg aacctggtcc agacttaagt atttgttatc gtaaataccg
     4981 gtaacagcca ccaccacaaa ctgattgttg ctgttttcca atacagtaga cacatctcct
     5041 aacttgttgt tgaatacaaa acggacggcc tgacgcattt caggaacatt accgatggta
     5101 ttatctgtgc tttttaccac agcggcatta gaaacctggt aaccggcctc tctggcagcc
     5161 aacataaaat cttcggcttt ggaatttcct gccatgagtt tgctcagttt attgtattct
     5221 gcactatagg ttttggagct tggtgttacc gataaataca attgagccac cttggattta
     5281 gctaccgctt tggttttatc ctgaatttgt actacatgcc aaccaaattt tgagtttacg
     5341 gttaaaaatt ggtttttata agccgtgaag caagcttcgg caaattccgg acctattccg
     5401 ctgtaagcgt caatttcgcg gaaccatccc atctctcctc cgtcgttggc agtccggtcc
     5461 tgggaatatt ctccggccag tgctgcaaaa tcctctccct tgcgtaaaac attgagtagg
     5521 ctgtcggcca attgttctgc agctttgcga tcgccggcgt tactgataaa gatttgccgt
     5581 gcttttacag aatcggaccg gttgattttg cccatcatac ggagcataat ccaggcatta
     5641 tcttcaaaat aagggccttt cacttctcca acggcagctt gttgcacaaa atctttcaga
     5701 cttccggaga attggttgtt ggcaatatag caatctacgt aaggcagatc cgactcggaa
     5761 ttaacggtag tagcgacgtt ttcttcggtg ctgaagtccg ttgctatttt atctattctg
     5821 gctttgatgg catcgttgtc ttcctgactc ggaagaatat caactaccac ataggcagct
     5881 gaacggtagg gttcctgttt gaagcgttct ttttgttcct cgtaacgacg actgagttct
     5941 ttaagagaaa ctttgaagct tgaatcggga acgctggcat aagacttgga agcatactca
     6001 aaatcgaccg aacgacggct cgcttcaaac tcatctttgg catccaggct gtttgctcct
     6061 acggctttgg taaccatgtt caccattttt tcatctaaac ggacgtattt aagattgttt
     6121 tcccagaaca gccagtaatt tttcatttgt ttgatttgtt cctgagcctc ggcagaatac
     6181 atggaaaaat cttcttgctg tatggttctg agaaaattca tcatcatatt gcggtcaaaa
     6241 cgtccggttt cgggattttg aaaaaagggc atctgttgta ccatgtagtg aacattttgc
     6301 ccgtttacca tatcgtaaag ttcttttgcg gtaacactta cacccaaatc ttgggcttga
     6361 tcgtccaaaa ggcgttcgcg aacgatggtt tcaaaaaccg attctcttat ttgatgcata
     6421 atatccgact gtaggttgtt ttgtccggtt tgcattttat acacttcttc catctctaaa
     6481 atacgagcat cgtattcctt gtaattgagc ggactgccgt taatttcggc aatgttttcc
     6541 tgcgattgcc tcaaaatggt agaaccggaa gttaaaaagt ctccgataat aaacgcaaat
     6601 aatgcaacac cgaccacgat caacaaaagg acggcctgac ttctgatttt ttgtaaggtt
     6661 gccatatact atttgtaggt tataattgtg ttctttaagc tattcccgag taccgtttac
     6721 aaaacagtat cgaaaaatcc cgcaaatgta caaaaaaata tgaaataaca cgcagcaaaa
     6781 ccaattaaag aacagccctg cacattaaaa tcattggtcg gaattttctt cttcgggatc
     6841 tattgggaaa ttgccttggg aattgagaat actgtatttt gtcatagatt cgttggattc
     6901 aaaacctatg ccggtggata tccaatcttt ttgttcaatg cgaataaaag aatccgaata
     6961 aattttatgc aagttttgat cccaatacaa caagggggtc tcaaattttt caccggccag
     7021 gttcatcacc tgtacatttt ttttgagctc ccagagtttc ctgttggtat aataacgagc
     7081 cgtatcgctt ttcacgatag attcaatgcg taagctgtcg tcaaagcgtt caaaatatat
     7141 tttttcggga aaaaaccagt aaggatcggg aagctggtcg taaactagcc aggaagctgt
     7201 aattactttg taacggatac ggcccgaatc tgaaatcaat gtcacaatgg aatcggccct
     7261 cataccgggc acagaatctc tggaattaaa attaggtaag atatctgctt tttctctttt
     7321 gcaggaaaag gataaaaaaa gtataacagt tacaaaagat gcaactgtta tacctgtata
     7381 ctttccaaat aaactcctta aaagcatctt tatttgttgc ggacaagggt gttttcctgt
     7441 atccagcctc cgattctgaa catttttcca acagtgagat ccgggtgcat aaaaatatct
     7501 tcggtatcgg gatactgttc tttatacctt gctatcaatt catctacttc atcactcaca
     7561 ctgggatcta ccgctttggc tttttgcagt ttatcaaccg ccgcccaata aacggctctt
     7621 ttaagaatag gatcatcaaa atagtcgaca ctgccggcgt acattttggc gatcagcaca
     7681 tagggagctc cgaagttttc ccggtattct gcagcttgca aagccatgga gcgggcttgt
     7741 tcatagctct taccggcaaa cagtgcactg gcagccaaaa aagcataatc ggctttccca
     7801 tccggatttg tttctaattc aatggcttct ttcagatatt cggcggcttt tttgtaatct
     7861 tcctttttga ttgcctgata agcgcaacct tctgccgatt ctgccgttgg attaacagca
     7921 tgtctgtatt ccgaagccag aaaataagta tctgtatctg tgcacttagc cctctttaat
     7981 aaagaaatga cgctattcaa gaaattctga ttgtctttgt tctgctcaat acgttctgca
     8041 aagagagcgg tcagtgtttc acagtcggct gctcctgaat ttgaaaagag cacatccaca
     8101 ccattcttaa tgcctacgat cctatctttt tcgggaccgg gagaggtaac agccaatttc
     8161 gcatcgagcc gttctaccgc tttgatataa tcgttaaaga aattttcttt atgagcatcg
     8221 tcggacaata aattcctgga aaccgatata aaggcggtag cataagccgg ttcaaaatta
     8281 gccgtgggcg aatcataaat cgccgttttt aaccactcgt aaggcagtct catgtcgccg
     8341 ttgggattga actgaagata atcgagtgct tttttcccga gtatccaggg agtaggataa
     8401 cgatcgtgat cgccgaaata cttgattcgc tggtcgtaaa cagccattaa ttcgtcaaaa
     8461 atctgagtct tttttgccgg atcgctttct ttgttccatt gccaggcttt gattttggca
     8521 ccgtcaatgt aaatacccac cctcgaagca gggcattcgg cgtatacaat ttcccaaaac
     8581 tccagggcgc tggcgtaatc tccttgtttt gcgtaaggct gatataaact cagattctgc
     8641 aagcagcgaa tactgtcttc gccatgtccg tacatggacc cgtcttccac ccctttttga
     8701 gccattaaaa ctgaactgaa taacagtcca caaaaaataa atccaatttt cctattcatg
     8761 tctaattaaa tctccgtttt acaaaccatg tttcactaaa ggcaaggctc aattttactc
     8821 tccagtaatg ttcctgaacc atgcccggca agggagtaag tattttccca tattccattg
     8881 ccagattgag agctgtgcgt tgatttcgta aaggcaaacc cagcccaaca ctaaggccta
     8941 tttcctttaa atttccgggt gcaaatttaa tatatgaatc cgaataatac aaacccattc
     9001 tgtatttaat tgcctgataa taacgctgtg ctatgttcaa tggcagaaat tcggcaccca
     9061 gagccaatcg agtacgcaaa gataaactgt cgcttacgcc aaaaaaagaa gtcttattcc
     9121 aggcctgatg ctgaaaatcc atccccaggg tcaacctgtt gtcgtaagta taggaaaaac
     9181 ccaaacccag attctgcgga aagccgaaag aaaggccggg atgctcttga atcagtgtgt
     9241 ccaaagtggt ggtagtggtg gtgtaattca cgtccatcaa tccgtcgggc gtataattca
     9301 ctccgagtac caggcggtgt ttatagccga accattgttc gtactggagt ccggcatcta
     9361 tattaaagtg attaacaacc aacacattgg aaacggaagt gggattgatt tcagcgttat
     9421 agggatagct gagaacagac ttattaatca gagatccaaa tttgtaattg atattcacac
     9481 ccaaagacaa atgcttaaaa aacaagacag aagtgcccaa ataagcatta ttgatacccc
     9541 cggaggcgga gtatgcgttg gaataaatca gagtattgcc gtcaagatcg gacaagctgc
     9601 ctgaagaact gaaagcataa cccaatcggg tatattcgta aaggcccaga cttaagcccc
     9661 aatctttgct caggggtatt ttgacggctg catactcaat attggaattc agtctggctt
     9721 ctctcagatt gttttcccta aaatgcgtat aggtagcgct caaaccgaaa tcaaacaaaa
     9781 aactcaagct gtctacggcg gaataagaag ccgggttcat aggattaatc tggcccgaac
     9841 tacgcaaacc gtaaccgatt ccacccatac cggaagctcc gccaaaggcc ggctcggaaa
     9901 tgattccgta tccgtagcga ctgtagggcg aaaaggtatt gttttgggca gaaaccgacc
     9961 agtaaaacag gaacatcatt aagcctgtta ttagggcttt taagcctgga cggatgttag
    10021 gagttaacat aatcgatgag tattctgtgt aaaccggtta aaactaagtt cgggcgtgca
    10081 aagatgtcac tttttagttt ccctgcaaaa taaattaaat cgcctccggt taaaaaggtc
    10141 aaaagttccg gatgtttctg tcggtgctga agtatataac cgttgatttc atgaataatt
    10201 ccctgcaata ccccggcttt gatggccgac ggggtatctt tgccgaattc cgggaaatcg
    10261 ccaaccgaaa tttcgggcaa gggcaaggcc ttggtatttt catgcaaagc tttcattctt
    10321 aaataaatac cgggggcaat attgcctcct tcatacactc cttcggcagt gagcagatcg
    10381 taggttatgg ctgtacccaa gtctacgata agtgaaggtt taccgggggc cagcgtccag
    10441 gcccccacgg cagcagccag gcggtcggct cccaagctct ggggttgaga ataattattc
    10501 tttacgggaa taggggtagt cgaatcgaaa cgaaaacaca ggcttacatt ttgtttaata
    10561 gcctcgtatt tgacatcgtc cgtatgattt aacacagatg aataaatggc tgcccggggc
    10621 ttaaattgct taaatatttc ccgggaaagc atttcttcag gatgccgggt atgaaatacg
    10681 gcagcaggct caagggcatc aagccgggta taatctgttt ttgccttggg aacattttcg
    10741 aaaaaggccc atttacaaac tgaattccct tgatcaatta tcagattcat tgttgattct
    10801 attattcaaa gagatccgcg tacaatcaaa cgagtaggta cataggtctg caaacgttcg
    10861 ttatgattga taaaacgagc cgcgagcaaa cccatttccc tgaaatcaac agaaatgcag
    10921 gttattccgt taccaattac ttccaacatg ggtgcatcgt taaaagtcac caggccgaca
    10981 tcttttccca gcgtcaattt tttggccctg caaattttaa ccgcctctat catatccgta
    11041 tgtgaaacaa gtagaaaagc ttcgcctttg cggatatcct ctttgcggat atgtctttct
    11101 atcaattgac tgcccaaatt gttttcttcg caaaagcggt gaaagaaacg tattcctccc
    11161 cgaggatgct cagaattaag cggaaacaca aaattcaact tttggtattt cagaaacaaa
    11221 ggactgccgg aactcaggca atcgtacaaa gtagtatcga acccctggca tacataagcg
    11281 taacccgatt tgtcgaattt tccgaaatcg atcaataaga ctttactgcg atccannnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaaga tacgtaataa cctttggtcg
    11461 gggcagattg aatggcaccc gaaagcttta attcctgaaa agctttgaaa accgtatctc
    11521 tggctaaatt gagctctgca ctcaatttgt tgatcgaagg cagcatttct ccctctttgt
    11581 attctccctg ggcaatagct tgtctaagag cgtctgccag ttgcttgact ttggtagtat
    11641 tggattgaaa ttctactctc atttttgggc tagtcgaaca caaatttagt tagataaaca
    11701 cttcctgccg gtgtcacttc gttggctctg acgatgtcga acacatattt agtcagataa
    11761 atacccgttg aatctgtaaa actctacttc tcaactccaa agtctttttg ttgttgctct
    11821 acttccttca acagggtttt aatttcctgc tcagacagct ttactctgtc tttgccctta
    11881 tcaaaccggt aatcttcctg atttgaagat tgatacgatt caaggtaatt aaaaggcaaa
    11941 cgggccggaa tgctttcaaa ttcaatctcg gcaggagccg gtgaatgctg tccttcgaat
    12001 aaaactccgg ccttgacttt gatttttccg gcttgcctgg tggaacgaat caataccggt
    12061 gccgatccga actccaccgc tctgggattg gccccaatgg aggcgtcgcc aattatttcg
    12121 ccctctcctt cgacgctgaa caaaatatta tctttggcta gtctgcgcac attgccgtta
    12181 tcgtcggtga cttctgcaat gactaccaga aaatcgctcc cgtccgcgat caattgctgc
    12241 ccgtcgtgat cgattcttaa tcttaatttg gtggagcgcc gggaaggcat ttttttagtg
    12301 ctgcacaccg ttttcccgtc tataatgcct tcggccacaa agctcacttg ttgccagttt
    12361 ttctgtaaat aactcaattc tctcatttgc cagaaatcga agacattttc gaatatgacc
    12421 ggtgcattgg gcataccctt atccttatgt agaaccggtt ttacaatggt atcctgctcg
    12481 cggcaaatca aacgcacgga atcacagttg ctgaatacca ccagatcggc attcgaaaaa
    12541 ggagaaatct catgagcaat aaaaaccatg gggccggttt cgatgagcgg atgttcaagc
    12601 tgcggtgata cctggctttt aaacatgtaa taagcgtatt tgggctgcct gaaacaatct
    12661 gtctgaccac cccagtaagg atcggggtga tagcccctct ggtgatcgaa agcgtgccat
    12721 tgggctcctc cgataaattg accgctggta cggaacattt cgtcgtagga atcggccaag
    12781 gacaaggcct gaaccaactg gggcaattct ccccaacttc tcgaagcccg gttgttgttg
    12841 ttttgggcat accagtcgtc gacattttcg ccaaactcac gggtgaaaat gcattgttcg
    12901 gccgctcctt cgtccttagg ccaaccgtac accactccgt aatgttgagc aacgccttcg
    12961 gaatgcatat cggcagctgc cagaggggct ccgggataag gaaattcttt ttgtgtaatt
    13021 tccagggctt tcatggaaaa gtccagcgga aaacgggttt cattcaaaat aggttcccag
    13081 agcaagaccg aaggatgatt ccggtcgcgg cgtatcattt gagtaacatc gtccaacacg
    13141 tattcctgaa attgagggtc cctgttccaa aactgccaac ccggggttgc cactatcata
    13201 aatataccca actcatcgca ggcatccata aaggcgggat cctgaggata gtgggcagca
    13261 cggattattt cacaacccgc ttcacgcagt tttttcacat ccctccaatg ctgggagttg
    13321 ggcatagcgt tgcccacgta ggcaaaatcc tgatgccgat tggttccgat caattggccg
    13381 taaggcttgc cattgagcca gaaaccttct tttcccttga attcggccag acggattcca
    13441 atacggctta ttcctccgtc gagcatgatt tttccctgcc tgacctgcga atgaatccgg
    13501 tataagtagg gagaactcgg agaccataat tggggttgtt tgacttcaat aagctgattt
    13561 atagtctttt tttcaccggc ttccaggctc aggttgttcc tgactttnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa atcgagggta tattgcgttt tacccggagg
    13741 ataagactta tcatcgctgt tgtcggcgca aaccgctatt aggcaagtat cgccggcttc
    13801 tactccaaat tcgttcaaat taacggcaaa aggcaaatac ccacccagat gctccagaac
    13861 ttgcttaccg ttgagataga cggtggaacg gcccatcacg gcttcaaagt acagggaaat
    13921 ttgctgccct tgcagagagg aatccgggac aaagcgcttt ctgtaccagg ctttgccctg
    13981 gtaattgcgt ccgccgctgc cttcggccgg catcaactgg acagtgtggg gtaaatttac
    14041 tatcggccag gcctgatcat caaaatccgg gttttgtgct tcggggacat ctccccggta
    14101 aaatcgccat cccggattga agttatacac cgaacgtcca ctgccttcca atgggaaaaa
    14161 gccggctatg gatatttccg gctgataagc ctgcaaggac gttaaagata aaaacaaagc
    14221 gataaataag actaactttc tcattattaa aaatttatag taaataatca ttcctgaatt
    14281 accagccggc tttccacatc ggccaacctt tctttggggc ttacttcgag gcgcagcaat
    14341 ttaccgtctt tcaattcggc ttcgacggta gtctgtcccg gagcatgcaa tttaaaatgt
    14401 acatcccagc cggcaggcca ggcaggaaag agtaaaatac gctccccttc cgtctgcaac
    14461 aacatctcct gcaagccaat tgaggcactc ccaccccagt tgtgatcggg agtccaatca
    14521 aagcccggcc cccagaaaca ggggaaacga tgctccgcat ctttgaattt caacaaagtc
    14581 agtttctttg cctcttctgt taaaccgaga caggcggcaa aaatattgta ttgcttccag
    14641 cctacatggc tgtaaaattc ccgtacgtcg ggatccagcc aataggtatt gatggctgta
    14701 tccttgtctt cggagacaac gttatatatt cgccagggaa acacgggata caactgcgga
    14761 acttccgtat tgtttattct ctcccaatgc agagccggag caatcacttt ctttccttcg
    14821 atctcccgga aactaatggg cggtatcgtt tggagcatat tttcccaacg ttgccgttga
    14881 tctgaactca gattttccag ggccagcaaa gattccaaca ccactttgag tccggcaata
    14941 caggtggttg aattataagc cattttatag gtctcacatg ccgaagtagg gtacaaaatc
    15001 aactgtccgt cggcattcaa ttccttgcta ccccgctgcc gtgccagaaa gcgatagtgc
    15061 tcatcgaaaa aagtcagcgt actttcaata aattccatat aagggccgat gtcgctgcct
    15121 gcgtagcgat gactttcgag tatcatcaga caaaactcca ataccgtctc ccattggtac
    15181 tccagccagg cattgtattc cagccccgga tcgtagtttt cgggacgctt ccatccgtat
    15241 tcgctcggat tgggcaaccc gaaatgttcc atctgctcgg tataacaggc tccgttgtgg
    15301 ccccagtaat ggttactcct taattcggcg tttttaaggc ttttacggta aaactcaaat
    15361 tgagggatca tcaaatcaaa atccccgctt ttaagcatgc caaaatacac gaggcgttga
    15421 ttctgagcgg taaaagtgcc acctccccaa ttgcggaaat ctggcgtgaa aggccggtcc
    15481 tcgcgcacgt gaacagggtc gaaggtaaac aagcctccgt tgaatttggt gggagcctct
    15541 ccacgaaggt tacaagccag catatagcgg aataactgat aattacgtcc tactttccag
    15601 ctttcggtat ctggttgttc cggatcaata caaataaaac tgcgatccca aaacgttgcc
    15661 caccaatcgg cattgagttg cttgtcgctg cggggtttaa ttaagccttt aatgctgtct
    15721 aagcccttgt gccagctatc aacttgctga gtagtgtgaa aaaccagacg gatggcgtga
    15781 tttttggacg gcttcctgct ttccagcctc caggccttaa agtccgtacc ggcgtaaact
    15841 cctgtgatgg tgtctttgaa cacaaaacca tcggcacaga gctcaccacc gaagcgaaga
    15901 tccgacagag gatccgtcat ttgttccttt acctcttcca taccttgttg ctgaactacc
    15961 acgtcgaaca tagtgccgga ggagggattt tgatgataaa aaacgatgcc gttttcgttt
    16021 atatcaattt tatcggcact ggtcttcaaa tcagccggtg gagcccattt gtaagagttg
    16081 ctctgacttt cgtttttacg gagcaagcgg tcgcggtatc gccaattttc gtaggagatt
    16141 actgccttcc ggggactggc agagcgaata tccagatgaa tgaccggctt aaacacatcg
    16201 gcccatacat caatgtccac tttattgcct ttactgccgg cctgaatgtt gaccaaaccc
    16261 cggctcagtt taagacattg cagaaattca accgtatcca aaggttcatc gggaaaactc
    16321 agccgaaggc gaccgccttt gaggaaacaa ctgttttcgt cgaaactgcc gctgcgcgaa
    16381 aaatacacca ataattcccc ctgctcaacc cagacattca tacctatgtc tccgccccca
    16441 cagggcatgg attcgttcga acctttactc tgagtagtcc aggtcagttc gtaatcttta
    16501 agtaaatcag caggtttggc aaagcccaaa gtccaggccg acaacaaaac caggcttaaa
    16561 aaaaggtgct tactattttt catgcttttc aaaaaatttt atttcatccc gggaccaatt
    16621 atcggttctg aaagaggaaa cggcctggcc gttcacgcca aataaatcgc cctccgaaat
    16681 attatcaaag gcgtaacggg ccgcaacggg tttgggaacc gaggcagacc atagctgaat
    16741 cttatcccgg ttgattttgg cctgagctgg ataaaaaacc ttgtcttggc cggctatcca
    16801 aaaatgcttc aggctgcctc ttgttctgtc gagacccagc cctgccctgt caaaactcag
    16861 tataagcata ctgtcgcgct gctcacaagc cgagtagcga gggctgcggt gttccgggct
    16921 ttccagtccg tagctgccca ccagggccaa taaggcaaga cgttccccgg cctgtcgttt
    16981 ttttctcgga tgaatatcgc taatcagacc ggcatccatc aacacggcca taccgcaatt
    17041 gggaatcaag tcttcggcct gtgcctgagc ttccctgagc agggcggcat tcaccgcttg
    17101 ttcgccctta ggtgtaatca gggcataatc gtaaggagca atctgacaat aatagaaagg
    17161 gaaatcccct atcccccaat ctttacgcca ggttgaaacc atttgcgtta agaggtcttt
    17221 gtagctttcg ggccggccgg tattggattc tccctggtac caaatacagc ctttaatgtt
    17281 cataccgaga aaaggatgaa ccatgccgtt gtacagggct gttgccgtat gagtggaaga
    17341 gcgcacttcc tcctgagttc gtgggatagg caaatccgac agaccttcga ggctttcggc
    17401 gctcatccat gcctcgataa aagatccgcc cagactgctg tttatcaagc ctaccggcac
    17461 tccgagcaat tcccgcaggt aacggccgaa ataataagca gtggcactaa agtcggctac
    17521 cgttccgggc gaagcttcct gccaatggcc ttgtacatca ttggcccggg aagccagggc
    17581 tgctcttttg acagtaaaca aacgcaactg atcgtctttt gaatgcatca ggtcctgcag
    17641 gccgccctct attccctgtg agggatagcc cttcatgggc atctccatat tggattgacc
    17701 ggagcaaaac caaagttctc ctatcaaaac attctcgagc aaaagttcct ctccgtcatc
    17761 caaaagaatg gtgtaaggcc cgccagcctc cggggtatta aatttcaatt cccaataacc
    17821 ttcgctattt gccttggttg catattgttt gttgtcccaa gaggctttaa gcttgacact
    17881 gttaccggat tccgaccatc cccaaatttt ggtttcctgt tgttgttgca acaccatatt
    17941 gctcgaaata aaggcaggca aacgcaattt tgcctccatt ttttcgggca acaccaacac
    18001 aaagagcaag agaagcaaat gaacgaaata tttcatacta ttgagcttga tttatcgtcc
    18061 atactaattt aatatcccct tgaatacctg actcttgcaa atattcaaca gggaagtttt
    18121 gcaaactgaa accttctcgc gaacgaggtc tgttaactct ccatatacga gcattcgtcc
    18181 agtaatcgtc ctcctgaatc agcttgtgat tgatggcctg taccctattg taccaggtat
    18241 tggcaacatt gattttcaac tcgttttttc cggttttcag tgctgaactt acttccaata
    18301 agtagggttg ggtccaaaca atgccacagg attttccgtt cagaaaaact tcggccatca
    18361 cattcacact gtcgaatttc agatatattt tctcagcttc gggaattttg tcaagtgtga
    18421 aagccgaatg gtattctgct gttcctgcgt aatatttaat atccatatcg tccgattttc
    18481 gccaatcgaa taaatccgta ctctccatgc tcttannnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnn nnnnngaatc gaatcaagct ggttggatat aaaccagatg tcgcttgctc
    18661 cctctctgcg atgattccag gcaagatcat acaattcttt gccctgtaaa tctttgaaca
    18721 ggacatcggc ttccaaaccg aagcgattca tgttttcttc tttgaaaggt aaaagacaag
    18781 cttgttgttg agctttttcc caaatggcat gagtcagttg ctccaattcc cggttgcgtt
    18841 gctctttttg atgataatcg ggaatgcaga ccggacgttc agaaggtagg agtacaatga
    18901 ctccggcatc ttgtagttct tttattttgc gggctacctc gagactcata tacccggcat
    18961 cgggcgccat ctgatgcggt tcgggaaata ccaacactct gtaaccggct ccgccgggta
    19021 aaaccatcct gccgttttcg gcccgggcca atcgcaagaa cgcgtctttg ttgaaagaat
    19081 cgtatttata gcctctcaga ggatctatcc attggttggc cgtatacata ttggccgaag
    19141 ccgttacatt gaccgaagta gaatgcacgg gtgcgccctg gttcaatttg cgaagctgtt
    19201 cagaggcaag ggcttcttct ccaaacaagc cgggcaacga agccaccagc ctatcgggta
    19261 aaatggccct gcggggaagc tcctctcccg taaacacggc aatgtcgttt accggaacac
    19321 cccgctgtag catggcctgg caacggctaa ggtaattgac ccactccgaa gcatagggcc
    19381 accatgtttg ccctctttgc ataaacaaac ctatgccgtc cagggtcatc ccggggtatt
    19441 ttccaataaa gggatgatga caatacacgt gaaagaataa tttgttcacc ccctgggcaa
    19501 aattgaaatc gcccaaggtt ttaaccatac ggggatgttc gttccattcg cctctcaact
    19561 gggtaaagcc ttcggcctgc acgagatttt ttccgtaaat atgtgctccc gaaatggcat
    19621 cggccatatc gttgggctta tcgtgcgtgg gactgtcgag ccaatactcg cccatgggca
    19681 gatccaccct ctgataatgc agcataccgt cgctaagcat ggtcggggaa acgcattccg
    19741 aagaataaac gcagttcttc tgtgcggcca gctcgctcat ggtggcaaaa aatacatcat
    19801 tcaccaactc tgttacggtt tggcgaatat cgagcaacac actctccgaa atatctgcac
    19861 tgcctatggg aatgccggcg tagaggggca aatagggtaa aagatcgtag gaacggcgtt
    19921 gacggaattc ctccagaaaa tttggggtcc agttttggct accgcattcc caactatcgg
    19981 aatgcatatt tgttaatacg ccttttagta agctcttgtc cacatgttta taaacggcac
    20041 caaaccaatt gtcgaattgc agacggacgg cttcgggatt gaacttatcg cattccaggc
    20101 ccctgccccc gcctccggtt tcattctcat ggcccgtgga agtatgtccc atcctcaata
    20161 tcagccagtc gccttgcggc aggtccagat tgctcaagtt gccggactca tccattttat
    20221 cgctgatgtt gataatatct ccaagttgaa cgcaattggc ggcagctaag ttgttctctg
    20281 tggttcgttg gcttaaacgc cagacaactc cgctctttcc ttcaaaattc tctatcaagg
    20341 gccggctgtt aagcctgatt ttggtaatac tgagttccgg tttccatttg gccggatcta
    20401 tgtcttcggc tgccggttcg gtaccttcgg gtgaccaata aaaacgataa tacctggcgg
    20461 tggtttcggg cagtgtgtaa gtggcgtccg catcggtatc ctgccagcct ctgcgtgcgg
    20521 gaacgagctg tttgacgtcg cagaagtttt ttccgtcgtt gctgacggcc acacgcaaac
    20581 gatgcgcctg aaagttattg cctcccgtca caatagtaag cgaacgggca gtaaaagctt
    20641 gatcgaaagt atattgaatc cagcaatcgt ctttagcctt gaagcgacct gagccgtcca
    20701 acaaaaagct caactcttgt ctatcgctgg aactgacttt gggccggagg ttttcgctgc
    20761 tctccaatgc aggagcaggc aaagcaaaaa cagcaatatc gcggtaataa tcgatttcgg
    20821 tttcaggctt gtccaatttg agttgttcaa tccggcctcc tttgatcagc gtttcggaat
    20881 acaccacctt ttgcatggac atctccggag taatccaggg accgccggca agggcaaaac
    20941 catcgcagat atgcataccc attttcagac ccaaacggcc ggcttcttcc atggaaaaac
    21001 gtaccatctc ccaccattcg tccgagagtt gcaatacggt gttggacata tactgcactc
    21061 tggaagaatc gcgaatgggc attaaatagg caccttccag cccaatttct ttcatggctt
    21121 ccaaatcggc ggtgatcccc tctttggtta ccgcgccatt gatccaatac caaaaaatcc
    21181 aaggtttggc agcgtcggga ggatttaaaa aaacctcatc caacgaagct gcccgccggc
    21241 aggaaaaagt acctaaaagc aaaaagctta gtaggactat agcagttctc ttcatataac
    21301 ttattctttt tgtttcattg ttgcctgccc cgatgctctc aagggccgta caacaaataa
    21361 tttgattatt gtatcactta cttgttattg ttcctgtgtt acaagccttc aatcctgtaa
    21421 cgaacctgag ttttcttcac cgtatatttt tttaaaacgc ccggtccgca actactgtta
    21481 cccaagccta actgctgggc atcgatggaa catatcaccg cctttctggc ttgcaaatcg
    21541 gtataatgac gacaattgct caggtcggta gccagatacg gcaaagcaga gaaagaaaaa
    21601 acatcttcta ctgccgtaat ttttaaacct ttattcctgc tattcttaag aatcaaatac
    21661 tgtgtttctt cgtgatttcc actttcctga ggtctcggat aaggaaacaa ctgatcggcc
    21721 actgtcgacg accacagccc ggtgcgcata ccggttttac ggtcgggata agtttcaagg
    21781 ggccccgggc cgtaccattg cagttgctgc aggtcttcgg caaaagccca ggcgactccc
    21841 agacgcggca gaaccggtaa ttccccttcg ggatggaatt gatgttcgac atccatactt
    21901 ccatcggctc ctattctgaa gacggaagta cagcgtatgg tacctttgag gtaggtataa
    21961 aggctctcgg ttttgatttc gaccgggccg tccggttcag gattgcagct aaagttaagc
    22021 agcttccttt ggggcgtagc catcaagtga gtactccagt ctttggccaa ccagttaccg
    22081 aagccggcat cgttgtctgt cggggctcga tatgcctgaa aattcggctg aggaaacaat
    22141 gcttcttcgc gggagagcat ttcctgccgt ttaaatacca gagaaaccag ggctcccgtt
    22201 tgtttatccc attgctggga aaaatcctta ccttcaacga tcaaaatctg ttccttgtcg
    22261 tcaattccga tttttccttt tttgagttcc ggagccgtca aagcggcctt tttcagatta
    22321 aattcttccc aggcaagttc gtggcctttt tgggcccaca gggcatcttc tttgagttgc
    22381 agactcaaaa aaagacgtac atcgccccgg tatttttttc caaaagtcaa aggaagcttc
    22441 agttcatggc tgtctcccgg cgcaacggcg ggcaagtcca tattgcccgt ttctatggtt
    22501 ttaccgtcgg aagtcacctg ccagcttaag ttatattgat caaggttagt gtggtgattt
    22561 cgattggtta tgcgataatg atactcgtct ttgccttgtt cgaacaaagc cggctgatag
    22621 acctttttca cttcccagta tttaggcgtg gtttgccgct cggccagtat aacaccgttc
    22681 atgcaaaaag ccttggagtt aggataatct ccaaaatcgc ccccgtagga catttgaacc
    22741 cgtccgtctt cgagaggttt ggccaaagcc tgatcgaccc agtcccaaat aaaaccgccc
    22801 agcatacggg ggtgactgta gatttcgtcc caatattcct tgaaattccc caaggcgttg
    22861 cccatgcaat gcacgtactc gctggtcata accggacgat cgtcggcggt gttgcgtgct
    22921 atactcaaaa gcctttccca gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23041 nnnccggtaa acggatcgtg ttcgtgacgg tttactccgc gaaaacgtat gggtttcccg
    23101 ttaataagaa cttgtccctg cttaatttcc acactacgga atccgacttt cgtctgtatc
    23161 ttttctaccg cctgccctcc tttattatat aaacccaatt cgagggtata gagataaggg
    23221 gtttcggcag tccacaagtg aggggaagct acttccacat cgaaccagcc aaaactgcgg
    23281 gcaccgcgtt ggggcgttct ggcattgaga atattggaac gaatttcacg gttgagcacc
    23341 ggcacagcgt cttgttccag ggtttgttca aaaacggctt tcccttcggc atcgtacaaa
    23401 tcggccctca gcacgtatcc gtctgccgtt tgtccgtcgt ataccgatag tttcggttca
    23461 atcaacagac gggcatttgt gtaagtatca tccagttcgg tacgtacacc aaagtcgtaa
    23521 atgcggatct tttcagtagc gtacaacgac acatcccggt aaatacccga caaacgccac
    23581 atatcctggt cttcgaggta actgccgtcg ctgtagcgaa atacctgcac ggctatcagg
    23641 tttttcccgg gctttacgca gccggtaacg tcgaattccg acatctccat actgccctgg
    23701 ctgtatccta catactgctc gttgacccat acattaaaag cactcatgac cccgtcaaag
    23761 cgcaagaata tttttttccc cgaccaggcg gcaggcaagt caaaagtacg cctgtaggaa
    23821 cccgtcggag tcctttcgct aaaagaagta taccggggat cgggctctga gcttaccaac
    23881 ggcggttcga ttttgaacga ataacctgca ctgatgtaaa tcggagtacc gtaaccatgc
    23941 aactcccagt tggagggtac cgggatttcc gcccagccgg catcgtcgta atcgttccgg
    24001 taaaaggacg ttggggcttc ttctaccttg gggacgtatt gaaatttcca gttgccgttg
    24061 aggctcatcg ttctgtcctg cttttcctcc aaataaggaa cgtaagaagc tctggcaggc
    24121 agtctgttta tttccaatac tctatgattc tcccagagag ggcggaatgc ctgagcacta
    24181 cacaggcaag aaccgattat aaggccggcg atgagcaagc cgatgttttt cttcataatg
    24241 aatgtattaa cgatccaagg gcatccaaac tgtcatctct tcaaaacccc tgtttccgaa
    24301 agcataataa ggaatcaatc gtaccggcac tttctttttg gccgaagaca cttctctgta
    24361 aagtacatta tcccagtttt gttggtccac ataccaggct tctcccttga ggctcatcag
    24421 gcggctgccg tcgatttcca tcatctcggg ttgaaagcta atgtccgaag gaatgctgat
    24481 attatcgatt ttatagttcc ccagatcagc agactccagg caatacacca gaggtcccca
    24541 cataacggct gtctgattgc gggtttcttc gagcaaggga ttggattcaa tcaatctggg
    24601 ctgaatacgc atgttgaatg ttatattatc ggtctttttc cattttgcct gtacccgggc
    24661 atagcctttt tctacctgta attctaccgg attaccgtta atgctgagtt cgtaatgatc
    24721 gcaccatccg ggtattctaa aaaacaggct gaaatcggcc ttgcggggaa gcttggcaaa
    24781 cgtcagctcc actttgccgt cccaaggata atcggaacgc tgaacgatct caatatcgga
    24841 accatccgcc aatttggtgg ccaagttact ggatccatag aggtttagcc atactccctc
    24901 tttggaaagg ctgtaagcgt aattctgagc ttcacaaatg gtacgcaccg tgttgggcgg
    24961 acaacaaaaa cagctgatgt attctgtacg ctctttgggc cagcgcaaca catagggaaa
    25021 ctcttttgac aaacgcagag gattggtata aaaatacttg gtcccgccta agctgatacc
    25081 cgacaagaca ctgttgtaca aggtccgttc caatacatcc acgtatttgg catctccgcc
    25141 gagcagaaac attctccagt tgaaaaacat atttccgata ttggcgcagg tttcgttatg
    25201 ggcggtagaa ttgggtaatt ggtaagccct gccgtaggac tgatgtactt tttgtatgga
    25261 atcgggcgta tagttggtcc catcgggcga agtgccgtcg tacaaagctc cgcaaccgcc
    25321 ggtaatgtac attttacggt aaacgatatc cgaccagatt gactcgagat tttccaacaa
    25381 taaggcttcg ccggtttcgg catagacatc cgccaccccg gcgtataaat aatttgccct
    25441 gacggcatgc cccatagcca gtttttgctg acggaaaggc actctgtcct ggttgtcgtc
    25501 ggttccgttt tcgaccatac ccctgatatt tatcaggttt ttagataact ccaggtattt
    25561 gggatcgcct gtggtacggt acatttcgac tacgcccata taatgagagg gacaaatggc
    25621 gttgcgcgcc aattcggcgg tggctgtttc gtagaaacgg cacaaaaaat cggcggcctt
    25681 gatggcaagt tccagcatat tgcgcttacc ggtagccctg taatgcacgc aggcagcagt
    25741 catcaggtga ccatgattgt aggtttcaaa attcaacctg tcgctgaaag ctcctccctg
    25801 ggcagtccca acagcagttc ccaccggtcc gctttgttgc gaagcttgtt ctctttgtct
    25861 tttacgctcg ggaatgatca cctgagtatg catgtagccg tcttcgcgct gggctttggc
    25921 aatggtggta atgattctgt ccatcaaagc atccagtttg ggatctttgg tgacggcata
    25981 cactgctgcc gcactttcga accacttgaa aaaatctccg tcgttaaacg gagggccgga
    26041 atgccggcct tcggcctcgc cggcagcaat ttccagattc ctgaaagcat gtgaaacttc
    26101 gggatcgctc atcacatgcc acatgttaat taccatagta tcgcggcaaa cctcaaagcg
    26161 ttctccccaa aaaccatctg tccaacttac agccgacaca tcggtgttcc atattcttgc
    26221 gtaagggctt ttcgtggtgt ttaccaatcc tttgtcctgt gcatacaggc cggtgcatgc
    26281 cagcagggca cacagcataa tcgtcttttt catgcttttc ttctattata attgtacgtt
    26341 aaaatgatat tctccggagc ccaattcaat caatgtgcaa ttgttttcgg ttcccagata
    26401 acgaatagtc cgggatgaac cgatgggccg gtcgctttcc tttatattct ttttttgaac
    26461 agcaggtaaa taaaccagag ctgaagcatt ggccggtact ttgatagtcc agtctaaata
    26521 tccctccgat tttttccagt tcgaaaccac caatccgtag ggcgttttat aggaggcatt
    26581 gataaagttc aaatcggcaa ttccaaaatc gggtttcatt attattcgct tgtaggccac
    26641 ttgttcggta tcgctgctga tgccggccag gttttcaaaa caccaaatca gcaggtcgcc
    26701 cagcaacata acatggttac ccgaattcat gctgggagcg gccgtgtcgc cattccacaa
    26761 ttcccaaatg gtagtggcgc ctttggtcag catatagccc caactgggtt gttttgtagt
    26821 gctggccagc aggaaggcta tgtctcccct acccatggca gtgagttcac gcatcagcca
    26881 tgaaccgcca atgacaccgg tagccatggt acctccgtag gtttccaaca gtgttttctg
    26941 aacattttct tccacaccct tccgggcttc ggcgggaacc agatcaaaag ccaaaggcag
    27001 gagattggct gtaaccgtat tgttgctgta gtactgtttt tcgctgttgt aaaaatgccg
    27061 gttaaaagct gtcttcatgt cctgagccat aatcagatag accgtggcat cttccggttt
    27121 attcagcaag ttggcgaaac cggacattat atgatacagc ttgtagaaat aagcggtcga
    27181 tatcagtgcg ccttcggtaa tgcgcgaagg atccttggag tgtatgattt cgggagcttc
    27241 gggaggcata caccaatcac cgtatttgtc ggcgaccaaa agaccgtcct tgagataatg
    27301 ccgacgcatg tgctccataa acagcttcat agattcgtaa ttcttttcta tgggcctcaa
    27361 atctccaaat tggcgataaa gcatctcgga aataaaaaag aaagcggccg gccaagtgac
    27421 gtcgtcggaa tagtaattcc aatacgccgg agccacatcc ggaatgctgc cgtcccaacg
    27481 ttgagcttcg cggatatcgt ccatccattt ggcgtaaagt ttgtcgtttt caaacaggaa
    27541 actttctccg taagcaccga tggtacgatc gcccagccag ggcatacgtt cattgcgctg
    27601 gggacagtcg atgggcatac ctttgtaatt acccttgatg ccccagtaag cattgcggac
    27661 aatgccgttg agcacctcgg acgaagattc aaaatacccc aggtcttcca tttcgtcgct
    27721 gacgaattca cctataaaat tttcgggctt gggttctccg ggataattgc tcaattccac
    27781 gtaacggaaa ccgtgagttg taaaaatcgg agcccattct tcgccctgag gatcgccttt
    27841 caatatatat ttatccgtca ccagcgcatc tctcaaattg gccatgccta aattgccgtc
    27901 ttcctgcaaa atttcggcaa aacgcagttt cacctcctgt ccggccttgc cttttacctt
    27961 catacgtatc cagccggtca tgttttgacc catatccagg atgtaggtgt ttttattaat
    28021 cctggtaata gactgaggat gaaatacatc gcttattttc atttcaggat tgggctgggc
    28081 gcgtataact gctgtgggaa tggtcacccg ttgagccgga aaccaatttc tgccggtttt
    28141 gaatccgacc tcagtccaac cgggaaattc cttgcgggca tcgtattctt caccatcgaa
    28201 ttcgttgttg ctgataatgg ggccgtcggc tgtcatcatc cagcgttcgt tgctgcctat
    28261 cgtctgtctg gtaccgtctt cgtactcaat gttcagaatc attctcagtt tgggataacc
    28321 gaaattggcc catttgtagg gcttgtaatt ctggcgcata gcataatagc gaccgtttcc
    28381 cacgatcact cccaaagcgt tctgaccttg ttgcaaggaa gacgtcacat cgtaactgtt
    28441 gtaaagtatg cttctgcggt aatccgtggg agccggagtc attacatgat tcccgatgcg
    28501 tccgccgttg atccacaact catacaagcc cagcccggaa atatgtacca gtgcctgacg
    28561 caccggcttg gcgcaattaa attcattacg gtaatagcgg gccgaaagcc gggcatgcgc
    28621 tttttccacg tcccagggca tgggcccgtc caagcctatc cagcgactgt ttttccagtc
    28681 gttttcggtg aaaagaccgg tagcccatcc ggctgtttca ctctctactg cctccttgtt
    28741 cgcattggtc caaatctgca ctttccagta atagtaacgg ctgcttcgca attcggggcc
    28801 ggcataggga atcagaatgg aagcttcaga attttgcttg cctgaatccc atacgtctgc
    28861 ctgacctgca gccagttttt cctcgctcga agccaccata agcctgtatg ccgtttgtct
    28921 gactccacgg gcctctgttt gcaatttcca actcaaacgg ggattgggcg catcgatacc
    28981 aatcgggtcc ttgaacattt cacagctcaa ttccactact ttgaggtcgg gagcggaaga
    29041 ctggtcttgt ttgcaagata aaaacaccag gcttaccagc gctgatataa gaataagtct
    29101 tttcatagct tttattttaa atcgggtaat acggtccaac tcttatctac atattttttt
    29161 gcggcatcta cggcaatcaa tacaatgtcg ttgccttcgg cctgcggttt cggagattga
    29221 aaaagctgaa cgccgttttc aaattcaccc aggtaactga gagccccgct tgtgggatcc
    29281 atgtaccaga ccgacttggt ctggccactt attttggtta aatctatttc catgggacga
    29341 cttgtataat tgtaaaccaa caaataatcg ttaccacggg tagcgctgag gcgttcataa
    29401 cgaactccgt tttcaccggc tatgaccgac tgatcgggaa tgcgttcaaa aaagggaaag
    29461 ctcaaaatca ggtttttcaa atacttcatt tgattaaaac cctcgtcttt aagcgcttcc
    29521 acccaatgta ccgtagcacc gtaagcgccg gggcgcggac ctcctttgta catctgcatg
    29581 atggaactgt tgccgtaagt atggccgaaa gaaccggcaa atacggacca ataggcgtaa
    29641 cgacgaacat ccttgccctg ccagagaggt tctttcggat cgtgcagccc ctggggtata
    29701 ccctcgtagc tgggttcgcc gtctatcacc ggcttaagcg gctcgtaggt cttgctgatt
    29761 tcaacgtagc gccagttgtc ttcttcggtt gaatcgggca aaaattcctt acccggctcc
    29821 gattgttcgt agcgccggtg cccactttga aacatattga aatccagcca ttcggcattg
    29881 tgcatccaaa tggccgataa ggtgcggcca aagggatgaa aagtcatcaa gtgattcttg
    29941 tcgatggctt tgatgctttc ggccagagca tgccagactt caggtttaac atcgccgcga
    30001 atatcccctc cgataaacca gataatattg ggacgatcct tgtagcgttc tgccagaaaa
    30061 gtgccgtaag ctctggcttg ttcttcgtcc atcaggcctc ttttgaccaa acctccccaa
    30121 atacatacca taccgatgta tattcccttg gattctgcgg tttcgacgat gtaatccatg
    30181 tgatcccaat agccgtatac gccttcctga ttgatcttgc tgaaatcaaa tccgtctatc
    30241 atggaaagct gaccgtaggt attgaccgca ggcaccccgt tgatggtttg cacctgaacc
    30301 acgttgtagc ccgcctgacg gcagttctcg agataaaact ccgcttcgag ccggttgagt
    30361 ctttcgggta acaaccatcc ggtttcaccc aaccaaaaaa atggtttgcc gttttcgtgc
    30421 tgtaaatagc attgagtagc gtccacctgc aatttcccgt tatcccaggg caattcttct
    30481 ttttgtccac aggccagaag cacaagcgtc agcaccaaca ggctgttctt taaaattaaa
    30541 ttcttcagtt tcatttctac tttagcttaa ggtaattgtt ttataaattg gtgttttcca
    30601 ctttgtataa gctccggcat atcctcacca ggtaaaaaca ccttggcatc gcaattggct
    30661 ggaatctcca ccgccattgt gaactgttta gcttcgtcga tttcccattc gacgccgatc
    30721 gtaccgtaaa gattttgtgt acggcatttt acccgggtca gatcacccac aacctcgggt
    30781 tttatgatca ggtgctgagc tccgggggct tgttcgtgat caaactgtat gccgcccaat
    30841 gaacggaaaa accattcatc gatcgaaccg agcatgaaat gattccagga agcaccctgt
    30901 cggggatccc attgttcggt cagagtagtg gctccgaact ttacctgaaa accgtatccc
    30961 ggcgtttcct cgtgattgtg cattttgtac atcagctcgt tgagcccgtt gcgggccaaa
    31021 acctgaaaca tataacgatt gccgacttcg cctgtggtca gcctgtttcc gtgagcttcg
    31081 atatcggcca ccaaacgatc gagtacccgc tgacgatagg gtggttctac catatccaaa
    31141 aataggggta aggcatggga agtctggctg ccggtgccat aaatacaact gtctgccctg
    31201 aaaaagcgtt cgtgaaaagc tttattgact tcattgccaa gttgggtata atagtctata
    31261 tcttcccgga ttcccagcat cgtggcagca cgaaccaaca attgaatgtc gagatacaaa
    31321 tgcgccgtac caaccagagg aaccggggtg ttgcgcgaaa agcccgattt gaaatcaccg
    31381 taatcgtacc agtctcccaa accgtgcatc actatgtgat tgtccgacct gctttcgagg
    31441 taatcgacgt atttcttcat gacagggtaa tattccctga tcaggcgatc gtcgccgtaa
    31501 cgccaataat acaggaaggg tagtattacc gannnnnnnn nnnnnnnnnn nnnnnnnnnn
    31561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31621 nnnnnnnnnn nncataatct ttaccggatt ggctgtctgg gccaacaata cgcccttagg
    31681 agcctcctgc accaccactg ctctccagtt gctgtcgtca aaaccgggtt tatcccagcc
    31741 ggtctgttcc aaattggcat cgtaatcttc gcccccataa atacaattga aggtaatggg
    31801 actgaaatcg tatttccagt tttgatccga gatccattcg ccggtacttc catcggtata
    31861 ccggacgaag agtttgaaaa acaaggtagg aggaccaaaa ctcacgagca atttgcgata
    31921 acgccctccc tgaacattga agaagccatt gcccagcaat acaccggcta cgttttcgcc
    31981 ttttttcagc aagtctttga catcgtacac attgtaatac accgttttgt cgtaatcggt
    32041 ccataggggt gcaaattcag aatcacccac cttttgtccg ttcagactca attcgtaatg
    32101 cccaagccca ctgatataca ccagggcttc ttctatgggt ttatccagat tcagtgcccg
    32161 gcgcaaataa aggctttgtt tagacagagg gtctgtagcc gcccacaaag ccttactttc
    32221 gggatcgcgg ttaactaaat ttccctcaaa ttggcggcct tcgggcaatc tggagtcggc
    32281 ccggcttatg gctcctatcc actgggcgtc gagccaggct tcttcaggtt tagtccaaaa
    32341 ttcctgttgt ttactccagg ccgtagtacg accctgttga tcccaaatgc gtactctcca
    32401 tgcgtatttt tgacgggctt gcaattccgg gccttcgtaa agaacctgct gactgttgtt
    32461 ttcagtgact tttccgctgc tccaaagcag ggatttttga gaggcttccc atcgataaaa
    32521 ttccagttcg taggcttctt gcaccactga attccccgaa gcaagaattt gccaacccag
    32581 gcgaaaatct ttgttttgca acgtcaaagg cccttcctga taatccaggc tcattttacc
    32641 aatccggatt tccggtacac aggaggaaag gctgccggca agcagtaaaa gacccaataa
    32701 aagttgaaat cggcttataa agctcttcgt tttaacaaaa tcgatcgatt tagactgcct
    32761 gtttttcatt ataaagtatt gaattattga ttaacactaa ttttattcaa ggcctgttct
    32821 ctgagctaaa acgattttgg aacagcacag accacataac acaacatgct gaaaaacaac
    32881 gatataacag aaaaatactg ttatattcgc tgcctgagcc aaaagtatta aaaaataatc
    32941 tgaaaaatga ttagatttag gctcttaaat aacaatttat cctaaattgc gagagacaat
    33001 acaaatgagc cggaaagaag taaaacctaa cactttaatt cactatgatt gagtctaaaa
    33061 ttgaagaagg cctgaaggag atgagggctt tctaccgcac taacactacc aaagattacc
    33121 gttaccggat tcattcactc aaaaccctct cttcgtccat tgccaaacac gaaaacgaat
    33181 tgatggagac attggccaca gatttgcata aatcgcattt tgaatcgttt gccacggaga
    33241 tcagcatagt acaacaggaa attcgttttc agatcaggca cttaagaaaa tggtcccgaa
    33301 ccaggagatt ggctacccct atggtctttt tcccttccag aagttacctg tacaaagaac
    33361 cttatggcgt ggtacttatc atagctccct ggaattatcc tttccatttg ttgttcaacc
    33421 ccctggtcgg agccctggca gccggtaatt gcgtcatgct caagacttcg cccctggctc
    33481 cggctactgc cggagtgatg gaaaaaatcg tgtcctgctt tcccggggat tatatcagca
    33541 tttggcacgg acacagggag gtgaatcagg ccttgctcaa agcccgtttc gattatatct
    33601 tttataccgg cagtccggcc gttggtaaag tggtaatgga agccgcctcc aagcatctga
    33661 cccctgtcac cctggagtta ggaggcaaga gtccctgtat tgtggacaaa gacgcaaaca
    33721 tccgttttgc cgctagaaga atagcctggg gcaaaaccat caacgccggt caaacctgta
    33781 tagcccccga ttatctgttg gtgcaccata gcgtaaaaga agagttttta ttagagtaca
    33841 aacaagccat cttttccatg tttggcagcg acccacaagc ttcgcccgat tatccgcgca
    33901 tcgtatcctc ttcggcactg gcacgtctga gcggctatct taaagagggt aaaattgtat
    33961 taggcggggc gat
//
LOCUS       DAOSTD010000006        33671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1437_length_33671_cov_12.939434, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000006 DAOSTD010000000
VERSION     DAOSTD010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33671
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1437_length_33671_cov_12.939434"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2679
                     /locus_tag="PLI37_00785"
     CDS             <1..2679
                     /locus_tag="PLI37_00785"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOQ57585.1"
                     /translation="DFQTALEPALALLNDSTAAQADIDQQTDQLHQAYLDFLDKRLGI
                     TDPWQADNYMHLAASTAQWAHYNVHDPTVVRSRGYFYEYGTDAAWGYSVKGIPYRRSR
                     DLVNWEYRGTVFDAYPQEVLNWKHSVDPNNSRTMTGIWAPYILKVGSEFRLYYSAIFS
                     DAARAMIVLATSNSPEGPWTNRGWIVHSTNTTVFNAIDPTVTIGKDNSHWMLWGSWTQ
                     GIYAIELDPATGLRKGDLNSYKRITRNGPQNTWGWTSSYEGPEVIYHPELDYYYLFIA
                     HGALGNVYHTRVARSKNPDGPYYDYYGNNVNYTVNRDIYPLICYPYQFQNHSGWQGIS
                     HVSAFASGGDYFLMNQGRPSFLPTMMIMHTRKIFWTSDGWPTLSPQRYTRPGIMPAIT
                     RDSIPGKWEEIRLYELMMSNGLAEPLGDDDAPDKFLCKSNTMTFNADGRFSSNFGSGS
                     WNFENDTLYIKRDKESKRIPLIPFYELDWENHRPSLLFTGINPNNGRSLWAKKVPENV
                     VSYNWVTNGSFDEGLKDWALNTYGGSFSESVVSTTYGSGNSFYVKVNSPSANYWDREL
                     KWRVPVRRGMRYKISFLAKSNSQEPFHIEMQRLNGSAYNLRTTFVCNTAMRTSTFITG
                     DAGESSGIYVLNFQYGTHPAGTELWIDEVKLEELSGKDYGNYVTNGDFETGTEGWNMN
                     YSGNVAVGLDRHNPLSGQASLYGKVGNASSLIRSMAGWKVYLFKGRQYRLEFDARGSE
                     GSVLSVFFDTQTAKRIENTLHPETRHYSLLLDSLSESKEYTLNFSFSKNQQGSECWID
                     NIELCDYKEPAAIVNPGGQSENHALLYPNPGKGLFYLDEAAAPAGSRFYIYNSAGILI
                     KELGLRGRELDLTELQAGLYYVKWQGRQQLQKIIIQ"
     gene            2694..3464
                     /gene="argB"
                     /locus_tag="PLI37_00790"
     CDS             2694..3464
                     /gene="argB"
                     /locus_tag="PLI37_00790"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOQ57586.1"
                     /translation="MDKLNIIKVGGKVVEDPAALEQFLQDFAKISGHKLLVHGGGTLA
                     TELAKQLGIENKITEGRRITDAETLKIVTMVYGGLINKNIVACLQAKNINALGLTGAD
                     LNLIRSDKRPVKEIDYGYVGDVKEVQGDLLAELLKLGIVPVLAPITHDKNGQLLNTNA
                     DTIAAETAKALAEYFDVHLIYCFEKKGVLMDPEDDNSVISEIDANLFKEYVEQGIIRE
                     GMIPKLQNAFEAIHCGVKDVTITKAQDILLGKGTRIML"
     gene            complement(3481..4692)
                     /gene="ftsZ"
                     /locus_tag="PLI37_00795"
     CDS             complement(3481..4692)
                     /gene="ftsZ"
                     /locus_tag="PLI37_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOQ57587.1"
                     /translation="MIDELIPFDLPSGEPAIIKVIGVGGGGGNAVTNMFRKGIRDVNY
                     LICNTDKQAMLNSAIPDKLQLGPKTTKGLGAGNHPEIAKAAAEESMEDIKAAFGEHTR
                     MVFVTAGMGGGTGTGAAPVIAGIAREMDILTVGIVTIPFRWEGESKINQALDGVEEMS
                     KNVDALLVINNEQLFRIYPDLTVKNAFLKADDTLTIAAKSIAEIITVPGYINLDFADV
                     NTILKDGGVAVMSAGYGKGQNRLTEALNSALKSPLLNNNDVFRARKILFNITCSDKSE
                     IMTHEMEQIRVFMDQFVNQVEEVIWGIAADNDLDDQIKVTMLASGFGIDDVPQVKVHR
                     QIKSRELEEDIEEKRRKRENRKSTYYPDDFGIPPRRLIYKPRVLSNQEMDNEELIDQL
                     DQQPANQRPFA"
     gene            complement(4701..6074)
                     /gene="ftsA"
                     /locus_tag="PLI37_00800"
     CDS             complement(4701..6074)
                     /gene="ftsA"
                     /locus_tag="PLI37_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOQ57588.1"
                     /translation="MNNVQSYIAVLDPGSSNLILSLGYKDDMGKVNIVSSKSRPIDTE
                     IRRGVVQNVDQVGLMVEELMRELLRDLDRGCSIDRVYVGLNLYSTQCIDSSEKLNLNS
                     QELITEEILSELFDDAIARSLPENKEMIAHFEQEYLVDGFPCLNPKGERPDSLEGRYK
                     LIAARTQYMKNLENSLSNAGLKDCRNILGIQASAEAVLSEEDKNKGAVVIDFGGGTTS
                     MCIYKDNVLRYVCVIPYGGKNISRDLKQLNLSFSQAESLKLEEGTALHYTELMKKEAL
                     NKTEEEIRFFSEPLHDDKEINEIVVARSEEIVENIWAQIIYSGVEVGKIGSGIIICGG
                     ASKMDGLEDLLRIKTQMPVRKADLSCRLSSSCRVEFQDPAYAQTIGLIMLGEKGGCRG
                     PVHSGQSKEQPRQATLPMEELEEITQKAPQKRPKTEAAKPDKKNKEKLISKIGNIFDD
                     LMKQGDY"
     gene            complement(6079..6813)
                     /locus_tag="PLI37_00805"
     CDS             complement(6079..6813)
                     /locus_tag="PLI37_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57589.1"
                     /translation="MAGRVFIWIGSVLMAGYVLFSVRLYSRQQNELQVQRVCIKVKDS
                     VDHPYVTSKEIGRLLKENQLQVIDKKLIEVDLSRIERVIDNHSMVKKAECFLSPSGCL
                     CIKIDQRIPVLRVYTGGGEAFFLDEEGKKMKINKRVPVDVPVALVQKTDSLSLAGLFE
                     LARLINKDDFWDAMIAQIVQESSGKLSLIPRLGELEIRLGKAENMEYKLRALRCFYEE
                     ALPRLGWDRYRCISTEFENQIVCTKK"
     gene            complement(6813..8189)
                     /gene="murC"
                     /locus_tag="PLI37_00810"
     CDS             complement(6813..8189)
                     /gene="murC"
                     /locus_tag="PLI37_00810"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOQ57590.1"
                     /translation="MTIKQVYFIGIGGIGMSNLARYYHSRSCRVAGYDRTETELTKQL
                     VKEGMEIHYCDDVEAIPEAFKEVNTTLVVRTPAVSDMHSELQYFRSAGFRIVKRAEVL
                     GEISRELDALCVAGTHGKTTTSTLLAHLLRQSSLDCNAFLGGISLNYGTNLLLSGKSR
                     LMVAEADEYDRSFHRLSPWMAVITSVDPDHLDIYHSHEAYLEAFAHFTSLIRPGGNLL
                     MKQSLVLQPRLAEGVKTYTYATEQMPHSSKADFRAENITIEEGRLYFDFIYPGGVLKK
                     MELGLPITIHVENALAAMGLAYLCGVSAEELRPALASFKGNQRRFDFQIRRNDFVYID
                     DYAHHPNELKACIRSLKALYPTKELTGIFQPHLYSRTRDFADEFAQALSGLDYVYLLD
                     IYPAREEPIPGVDSKLIFDKMTIEGKTLCSMDEILSLLDRKKPEVLVTLGAGSIDRLV
                     PLLKEKYS"
     gene            complement(8186..9328)
                     /gene="murG"
                     /locus_tag="PLI37_00815"
     CDS             complement(8186..9328)
                     /gene="murG"
                     /locus_tag="PLI37_00815"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOQ57591.1"
                     /translation="MNSRKKSKQQNNETRKPRFIISGGGTGGHIFPAISIANALRQKC
                     PEADILFVGAENRMEMEKVPAAGYPIIGLPVRGFDRQHLLSNVKTLFQLLRSMCKAHK
                     LIREFKPDVAVGVGGYASGPLLRAAAAKKIPCLIQEQNSYAGITNKLLAAGAARICVA
                     YEGMERFFPKEKILLTGNPVRQDLLRSLNQKEAALKHFGLKPDRPVLLVLGGSLGARS
                     INRAVQQQLESIEKSGVQLIWQTGKYYYEEIMAANAELNNKAILITDFISRMDYAYAL
                     ADLVVSRAGAGSISEFALLGKAVLLVPSPNVAEDHQTQNALALVRKGAAVMIGDEEAC
                     EKLIPEALALLEQPGRLQELRSAIKKMALPHAAEHIADEIMKLAKI"
     gene            complement(9294..10520)
                     /locus_tag="PLI37_00820"
     CDS             complement(9294..10520)
                     /locus_tag="PLI37_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOQ57592.1"
                     /translation="MELIQSVFKGDKVIWMIFLFLCIISLVEVFSAIGTLAYARMNPF
                     EPIIRHGLYLLIGVALVVAFHNFHYKYTALLVFPLLLLSVIMLILTPIIGVRLNEAAR
                     WIEIFGIQIQPSELAKLSMVLFSAWFLGRAQGSKEAMDKAFWVVLIVTAALAFLIVFE
                     NLSTAILLCLFVFLMMVVAGVDWKKLLILFLLVASAAALLLFLLIKVERLDGVPRWET
                     WHNRLVTEQLDVRDEGFKITDENRQVSHAKIAISEGKFLGVLPGNSSQRDFLPQAYSD
                     FIFAIIIEDLGWLGLFGVPFLYLFLLFRALKIAKRCTKSVPMLLVLGSALIISMQAFV
                     NMAVAVNWFPVTGQPLPLISRGGSSGVITCLYFAIILSVSNFATQKPIPEEENLVSNE
                     TEDPDYEQQEEIEAAE"
     gene            complement(10524..11858)
                     /gene="murD"
                     /locus_tag="PLI37_00825"
     CDS             complement(10524..11858)
                     /gene="murD"
                     /locus_tag="PLI37_00825"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOQ57593.1"
                     /translation="MKRIVVIGGAESGSGAAVLARKQGFDVFLTDNAPIPQRYRKLLE
                     DNMIAFEEGQHSLELILNADEVIKSPGVPDKAPLIQTIKAKGIPVVSEIEFAGRYTKA
                     KMICITGSNGKTTTTLLTYHILKNGGLNVGLAGNIGQSFALQVAQQDYDYYVIELSSF
                     QLDYMFKFKADVAVMLNITPDHLDRYHYNLQEYVDSKFRIIQNQTEQDAFIYWNDDPI
                     IQREIKKRTIRAKTYPFSLKKDAGIKAFIEDKILVINTPDQQFEIEQEALALRGTHNL
                     YNTMASAISANIAHIRKEEIRTALSNFKGVEHRLEKVAGVAGVEYINDSKATNVNSCW
                     YALESVNTPVVLIMGGTDKGNDYGEIEALVRQKVKAIVCLGVDNTKLHDFFEGKVDEI
                     RDARSMKEAVDLASSLATKGDTVLLSPCCASFDLFENYEDRGRQFKDCVRNL"
     gene            complement(11861..13120)
                     /gene="mraY"
                     /locus_tag="PLI37_00830"
     CDS             complement(11861..13120)
                     /gene="mraY"
                     /locus_tag="PLI37_00830"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOQ57594.1"
                     /translation="MLVPLFDYLNSIDFTGAGMFQYVSFRSIMAFIFSLLIATFIGRK
                     IIRKLQRMQIGDVPRDLDVEGQLSKKGTPTMGGIIIFLSLIVPVLLFAKLDNIYIILM
                     LISTVWLTLLGFADDYIKVFKKHREGLKGKFKIIGQVGIGLIVGLTLYLSPEVVIREN
                     VQLNNGLPNEEVRFEEGEVKSTKTTIPFFKNNNLDYANLVCFMKEHKQAAGWVLFVLV
                     TIFIVTAVSNGANLTDGLDGLTTGTSAIIGVGLGILAYVSSNMNFASYLNIMYIPGSE
                     ELVIYMSAFVGATVGFLWFNSFPAQVFMGDTGSLMLGGVIAVFAIIIRKELMLLFFCG
                     VFLLESLSVMMQVSYFKWTKKRYGVGKRIFKMAPLHHHFQKPGNAGIEALIQKPIKAI
                     PESKIVVRFWIISLLLAALAIITLKIR"
     gene            complement(13179..14633)
                     /locus_tag="PLI37_00835"
     CDS             complement(13179..14633)
                     /locus_tag="PLI37_00835"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOQ57595.1"
                     /translation="MKLSRCIEDLTVLEHLGPKDKEINSVCFDSRLAAPSCLFVAQKG
                     NRSDGHDYIPEVVAKGAAVIVCENLPSEINPEVTYIRTGNSNLALGLISAAWFSHPSR
                     KLKLVGVTGTNGKTTIASLLYNTLKALGYKVGLLSTVCVRVDEQELPATHTTPDAYAI
                     NSLLSQMLAAGCEYAFMEVSSHAVDQERIAGLHFAGGIYTNLTHDHLDYHKTVEAYLR
                     AKKSFFDRLPAEAFALTNADDKNGKVMLQNCRAAHYTYSCRSLADFRARLLETHSDGM
                     QLDFDGREVHVRFIGKFNVSNLLAVYGTAVLLGMDAAEVLRIISTLRPVSGRLEFLNS
                     PTGYTAIVDYAHTPDALKNVLQTLAQIRKQGKIISVVGAGGNRDKTKRPEMALEAVRA
                     SDTLILTSDNPRFEEPQAILDDMYAGVPAEARKRVLSIVDRREAIRTACRLAGKGDYV
                     LVAGKGHEDYQEIKGVKYPFDDKKIITEIFKELL"
     gene            complement(14642..>15376)
                     /locus_tag="PLI37_00840"
     CDS             complement(14642..>15376)
                     /locus_tag="PLI37_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOQ57596.1"
                     /translation="HKSFGTEVLNLKICSDKTLREIRAMLDSVVEKGTGEAVKSKFFP
                     IAGKTGTAVLSKGSMGYKEGGKNYQVSFCGYFPSDRPKYSAIVVIRDPVVGYASGGVM
                     AGGVFKEIAEQIYARELWIDLNSKRVKETKETGPPKIKTGLPGPSFYVMDKLNIKYDD
                     SGVHGERPIVARREEESKRLLLEERNLIENLVPNVLGMGARDAILLLEQWGLQVRLTG
                     KGKVVQQSLRPGTVSHEGQEIVLRLN"
     assembly_gap    15377..15476
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15477..16447)
                     /locus_tag="PLI37_00845"
     CDS             complement(<15477..16447)
                     /locus_tag="PLI37_00845"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOQ57597.1"
                     /translation="MDEQTGEDITKGIRKRYLIIVILCFSVMLFGIIFAAFHIQFAEG
                     AAWAEWKNKQQRENVVVPAGRGNIFSDEGLLMAATVPSYALYMDFQAVHRDTLNKYIK
                     PLCRALSQKFGDLSPADFEARLREGQRRASREYLIHPRRVSYADWKEIKTFPLFSKGR
                     YQSGLYEKKYLERQKMFGSLASRTIGNIYAEFGKGGRSGLELKYDSLLRGQDGVSTRQ
                     KVAGQYMNVNNKEPRDGWNLRTTINIPMQDIVETALKEMLQKTGAASGTAVLMEVKTG
                     KVKAISNLGLTSTGSYQEIQNYAVSDLSEPGSTFKTIAMMAALEEGK"
     gene            complement(16477..16824)
                     /locus_tag="PLI37_00850"
     CDS             complement(16477..16824)
                     /locus_tag="PLI37_00850"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOQ57598.1"
                     /translation="MEQEEENKKTTKRFIFGEFIDGSFLDKNFFRKHAKLIFLIVVLL
                     FVYIGNHYSVIMKLAEINRLQQELNDVKYEALMYTSEFMQESRQSHIKSMVEEKGLEL
                     KELTEPPYTLKVK"
     gene            complement(16828..17730)
                     /gene="rsmH"
                     /locus_tag="PLI37_00855"
     CDS             complement(16828..17730)
                     /gene="rsmH"
                     /locus_tag="PLI37_00855"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOQ57599.1"
                     /translation="METPYHIPVLLSESVDGLALKPGGIYVDATFGGGGHSRAILAQL
                     KGGRLYAFDQDEEVLKNLPEDKRLQFVHGNFRFLSNFMRYYGEEQVDGILADLGVSSH
                     HFDQAGRGFSFRFDVDLDMRMNQRATLTAADILADYSKEELCRVFRVYGEFRQAARMA
                     GAIVKAREEQAVRTAGQLSDLLRPLLSRQAEKKDLARVFQALRIELNQEVGALEDLLT
                     QSLDILKPGGRLVVISYHSLEDRLVKNFMRTGNLEGEVKKDFYGNNLSPFILINNKVL
                     VPSPEEQELNPRSRSARLRIAEKR"
     gene            complement(17738..18223)
                     /locus_tag="PLI37_00860"
     CDS             complement(17738..18223)
                     /locus_tag="PLI37_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOQ57600.1"
                     /translation="MRFLGNIEAKLDAKNRVFVPAAFRKVLDNAVFTKTDEGSQSRIL
                     YLRKDVYQNCIVVYPAPVWEEELSELRARLNKWNPQEQELYRQFMLEAEAVEMDPNGR
                     ILISKRLLLKTGIEKNVRFLGVDNTMEIWAKEALEQPRVDPELFKTRMQELMTKTKIP
                     E"
     gene            18703..>20701
                     /gene="metG"
                     /locus_tag="PLI37_00865"
     CDS             18703..>20701
                     /gene="metG"
                     /locus_tag="PLI37_00865"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOQ57601.1"
                     /translation="MTRQFKRTTVTTALPYANGPVHIGHLAGVYVPADIYVRYLRLKG
                     QDVLFIGGSDEHGVPITLKAKKEGVSPQAIVDRYHHIIKKSFEDFGISFDIYSRTTSA
                     IHHKTASAFFDKLNRQGSFVEKTSEQYYDEEAGQFLADRYITGTCPHCGNENAYGDQC
                     ESCGNSLSPNELIHPRSTISGSKPVMKATKHWYLPLDQYEAFLKQWILEEHKEWKSNV
                     YGQCKSWLDMGLQARAVSRDLDWGVPVPVEGAEGKVLYVWFDAPIGYISNTIELLPDS
                     WEKWWKDPETRLIHFIGKDNIVFHCIVFPAMLKAEGSFILPDNVPANEFLNLEDDKIS
                     TSQNWAVWLHEYLEDFPGKQDVLRYVLTANAPETKDNNFTWKDFQARNNNELVAVFGN
                     FVNRAMVLTHKYFQGKVPARGEPNDYDRQTMEEFTDVQNKVETLLDVFRFRDAQKEAM
                     NLARIGNKYLADTEPWKLAKTDLPRVGSILNLCLQIAANLAIVFEPFLPFTAQKLRRM
                     LGIDSFRWESLGGTDLLPVGHQLNPAKLLFEKIEDDAINQQIQKLLNTKNNNKATREN
                     QAEASWTPAPAKVPIDYDSFCKMDIRVGTILQAEAVPNTEKLMKMLVDTGLDQRTIVS
                     GVLEHFSPEELIGKQVPVLINLEPRKIRGIVSQGMILYAEND"
     assembly_gap    20702..20801
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20802..21935
                     /locus_tag="PLI37_00870"
     CDS             <20802..21935
                     /locus_tag="PLI37_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOQ57602.1"
                     /translation="KMKDQFNIYSSDSQASKVLPLSPEEFDFEAYAEYEARLNQGCKK
                     FWEAPSGVMVYRRMRVKEVFAEDSADWQKSLNWQLGALQQSMGYKADIPNFLEPWYGI
                     GTIASAFGFGYRWEQGQAPALDGKFEQLEHLLQADFKPVAQTAIGSHSLEMTEYFLEQ
                     TRGRLPMSWCDVQSPLNILCNLVDSNMFFPEFYLNPEAVEQALDKITDLLIEFTKVQT
                     RLIGPALVKPGHGFASSRFFDGFGMSDDNIVMLPPDLYNDFAVPRMVRAGRPFGGPVF
                     HSCGNWSDKKEDIVRIDGLRMADGAFSKATDPDANPCEGFAAAFAHTGIVLNARIVGD
                     LETLGQKVKELWAPGMKLIVVTYCENPSEQAAAYDLIHEICRE"
     gene            complement(22023..28793)
                     /locus_tag="PLI37_00875"
     CDS             complement(22023..28793)
                     /locus_tag="PLI37_00875"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57603.1"
                     /translation="MKKFCVLLLALMLLPTWLGAQTVFDNTTVNSTYVLSDTVLPTVA
                     NVDTAEAFSTTQFIMGPGLRVNTFKKQSSDLTATLFDEPNATALTIGGRLPDYVLTFI
                     LRPSKGVTFKPTNISFKAFKNGTGDQVKIDVMNQRGEAAETLLSSIDIARNNEAGGYY
                     TTVSLDITDGVASNDTYILKLYPWGKGMRAGKGCAIGDLVITGVVHGTVIPVPKYTIT
                     PLVSPEGAGEISINPPGTLFDEGTELNLIALRNFGFEFSNWKSSETLLTDSSVLELVL
                     MSDTTVTAHFTPVSTYELAINAPAGVKDYMFDLDPPFFPVAGKKMYETGTSVLVSPRS
                     NAIMDFTGWEGGSAGPDTTVNMTANVTLQANYDVKTYLAGWDFYVAGNKDRFADFASA
                     ENVVTKLMLINADGTSTSWLDKSEMLGASYEGKYCAVNWKPLADKYYYQTCVNASMYN
                     NIKVVASMLYNYNAYQKQLVEYSLDGTNFTTLDTIVMSTNKVWYTKTVALPQAADYAS
                     KVYIRWIPDYTSDIVGTSSTNDGTSITDIFVYAEGLKTDAKADITDTTVVNPNFDTNI
                     NGWTSTTGASSNKIATNKTGDFTGPFWENWKNTSFTGKMSQIITGLPNGSYLLRAAAF
                     CNNGGDGFYLFANSGKTQVQTTEPTFHEVVGVVTDGKLEIGLLAELPTNNWVGLDNVK
                     LYSLGFILNDMITALEELIVEAEAITGDMQASVATGLADAISNAEAAVASRSEVHVVA
                     SLTALRAAIDAANSSIALYAAFPALQTKANYIYSATAAEAEALQAAITTANDTYVAKT
                     ANQTTYDNLVAAMVSYRAANDVTAYVVNPDMTEGKTVGWASDFGEQTAAYPGFEGRFL
                     EKWVSAANNLADFNLSQIISDLPNGTYTLSAWCIATKQNQAGEAAKTYVKNVYLYANN
                     DSTPVSSLNGVPEEFMINVTVADNTLKLGFTGISCTANWVAIDRVRLMSHDVSTLLAA
                     KQVSLNALIGEAEAVVAGVDSAMLGAESFKYAPAKLDALNAAIDSAKAFTGASVNEYV
                     AMENFLTAAMNNLRQMNQPLATQFFYIVHSSLNHLDVADGVKIKTAADTAYYQWFKLL
                     PIGSGELNAYHLKTLDEKFVGLTGTNNWDMSAQDVASSDNNVKFIIEMIDGSKYKLRT
                     IKGLLGTDAVTSGSGVYGNKAAGANSEWSLVEVGEAVYQYHNSSVELKTVLDEANAQY
                     DSTLIAADDFAAAIAAAQAVYDKGTGASVAELQAAVVALNEAIAAFEEANEVPPIVIN
                     KDDYVAFYSQDYEFVAQASDVWTSPNNQAGFSLVEEAGNKYINYDHGNVNSRGAYSNF
                     DIDYAGISEYLVEFDLALTAGNNQATEFALKGTDFAYAGGNVNWGAEKGYLFKLSTTN
                     STTWTIVGSGAESYTVELNALEFYHFLALVRADGIQLTITNAADSVVLDNVAITSGSE
                     GIECVGLHYQAGRYQSKMKIDNILLSKPVPLTKVTWYMQDYENVASAADVWTSPNNQA
                     GYSLVEEEGNKYIYYNHGSVNSRGSYSNFEIDFTDVDKYILEFDLALKAGNNQATEFA
                     VKGTDFAYTGDNVNWGAGGGYLFKLSTTNSTTWTIAGSGAESHTVELNALEFYHYSAE
                     ISADGILLTITNAADSAVLDKAAITSGSAGIECVGLYYMAGRYLSEMKIDNIAFSKLV
                     KGVVMVEPTVLMTGVSGISRIITMLSTQPTAILKYVLVSAEDYEAAVEIADVDTTKYQ
                     WQTYTAPFMVDAEASYLLAYATDGSVNSALVKGVYAAGTEIAVNAPVISLVAVNGTAK
                     TYAITSDNSDVLFAPVSVINYEYGTNSGVYSGPLTIDESCTLTATASTEGYVSASTSI
                     AIDGRTNYRVAKSYDFTQVDQAVIDTMLAHGWTASYDSLNVLINIHLLEGAGSDKNAD
                     NLFYYDQDSVKIQYFPDFAANFDSYELRTGADGKLAGLYSQSSGGRAVTVSNITAGQF
                     VEFIIENVSYIVKSTSTSCSYTMDRYKTLQKVNVFIPDIYLDVNTVSIDITAAGNVNV
                     EYDKAMNIAQNEVLSITGTVNFSRVFGSGYEPFVAPFNKPVIMDADSNVLVRGTDYVI
                     RRLSSGEFVELGPTANIVANTVGYVIKVAEKYVGQPITFVYESETGFELGIAQTTFNA
                     PSSGIAYRTNRRFIPHALGSDTTTVYAYVMNDEGTAFVKTANPVIPAFQACIMADSAT
                     LATTDEIKVEKEQALNGISQDRVVVSREYFDLSGTRLLEPGVGINIERIRYEDGAIEV
                     KKIIIYAR"
     gene            complement(28837..30585)
                     /locus_tag="PLI37_00880"
     CDS             complement(28837..30585)
                     /locus_tag="PLI37_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57604.1"
                     /translation="MKKIYYLTFAFLFCLISVGAYSQAFIETDYVVEFPFDESDYAGE
                     TQVIISDATVTFTADAFNTCGLPFGTGTLITMEEGAEIIMFDSPETWFPDADAEENMD
                     YIDYLTDSEGQKVTSVPFNFLINGNSRIVLDSKCRFGGTITGSGNLTLVVGDSTILDS
                     DLGISSLGEFTGTLTIEVKEGNNQLLIGSTYPGTCPIDNVTYNNASARCYHSIPWTLV
                     LPDGIHVYTQDATHTCFPRTIGHAYVHCSSTCTFRSNQLHLYDLEIDGGADNRNFEMY
                     SVGQGFNYPVNYLGAYAYVRTGALYVNGDKASFSNIINRISIRGREMFVGGVGFIDCT
                     VEGKAGTTAHIKPGATGENDIGTLTILKELYLNSNNSVDIDFGPGGKSDKLVLVGGGA
                     FSGEFTRLWVNIMDDFYDNPVAGDYKVLDAPTLRMNEVPVLDTTFTGTFTTTIDSIYS
                     GDTLIRVDTIVTADIERIDTIGWEISNNLVVNAGQLQTGQHKDSLPEGYAYNFDRFFS
                     EGIIAITGPGYEEGNIVNGPSGINQVKLPDKFVKQREIFNLTGVRTKTLTRGINIIRT
                     TFSDGSVEVQKYFYDE"
     gene            complement(30638..>31018)
                     /locus_tag="PLI37_00885"
     CDS             complement(30638..>31018)
                     /locus_tag="PLI37_00885"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ57605.1"
                     /translation="PEPFYFDPRYTTTPYYRGPWRYNFGIRGRAWLQSKEGSVFADKT
                     LAECVDKQDSIRVMEKIIMDECALECAYEGHRFPDLVRVARRMNREAAGSGNNYLQAV
                     LQGKYLKSGRAVPDYSSEEKWFLP"
     assembly_gap    31019..31118
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31119..32143)
                     /locus_tag="PLI37_00890"
     CDS             complement(<31119..32143)
                     /locus_tag="PLI37_00890"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ57606.1"
                     /translation="MKTNKQIALVVLIGSLMLSSCVQTDIDDALEYDKHYKTTEDADN
                     AILGVYSSFMRMADQMLILNELRGELTDLTAYSGTELQEIDAANPSESNTLADPTSYY
                     NVILNCNDALENFKIMLENNRMTPEEFRERYADLTAMRCYMYLQLAAQFGRVYYITKP
                     IVNLADMRAYSDSAKVTIDQLLPLLIADMESLEFMDPYVTSPLVQYNLDGYDLSYFFI
                     NKKLMLADLYLWNGDYLKAAFLYKEIMDTYSDGEATVNCYTYKLAAATTWTTATAYST
                     WAYQIFFLRYKEDDMEAYNNQWVNIFSDDMTGRRIRYEWIWSMSYDAAYAPSFPLIDL
                     FAKPEDGG"
     gene            complement(32163..>33671)
                     /locus_tag="PLI37_00895"
     CDS             complement(32163..>33671)
                     /locus_tag="PLI37_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOQ57607.1"
                     /translation="RRLDASLGLRYLNSRAEDDYGKGYNSSSNYFVSIGSGSNRLYQT
                     GGAIGNWNWLALYGNVDYNHKNRYFVNALLSYDASSRYGSEIAAFQAFPSLGAAWLIS
                     SEEFMKNSQWIDMFKLRANYSMTGNDGIGNYAAKRYYVTQNFLGNYGLVRGNLVNEKL
                     MPERNNKLSAGLDLALLNERLNISLDLYHSTVKDMLIHAEAPAYTGFRFYIENGGEMR
                     NRGIDASLNARLVEKGAFSWDLGLAASHYQNEILSLSTGDFNTPIAGGLVRTEVGKPL
                     GIFYGYKTEGIYQTAAEAAENQLYKMSGTVKVPYGAGDVRYVNVNGDDLIDQNDMQQI
                     GNPNPDLHGSLWTDLAWKGLSISAVFRYSLGNDVYNYTRRNLESMSSFANQTQAVLNR
                     WKSEAQTATMPRAVYGDPMGNSAFSDRWIEDGSYLKLKTLSLAYAIPIQSGVLRNLTI
                     YGTAENLLCLTAYKGYDPEIISASAEMPLYYGVDAFTMPTCRTFYLGIKIGL"
BASE COUNT         8773 a         8448 c         7430 g         8720 t
ORIGIN      
        1 gattttcaaa cagccctcga gcctgccctt gccttattga acgacagcac ggccgctcag
       61 gctgacattg atcaacaaac agaccaactt catcaggcct atctcgattt tctggataaa
      121 cgcttaggca tcacagaccc ctggcaggcc gacaactata tgcacctggc tgcgtccact
      181 gcccaatggg ctcattacaa cgtccacgat cccacggtag tccgcagcag aggttatttc
      241 tacgaatacg gtaccgacgc ggcctggggt tacagtgtaa aaggcatacc ctaccggcgc
      301 agccgcgatc tggtaaactg ggaataccgg ggcaccgtct tcgatgccta ccctcaggaa
      361 gtactcaact ggaaacacag cgtcgatccc aacaacagca gaaccatgac cggcatctgg
      421 gctccttata tcctgaaagt gggcagcgag ttccggctct attattccgc cattttcagc
      481 gatgccgcca gagccatgat cgttctggcc acttccaata gccccgaagg cccctggacc
      541 aaccggggat ggattgttca ttccaccaat acgacggtgt tcaacgccat cgaccctacg
      601 gtgaccatag gaaaggacaa cagccattgg atgctgtggg gttcctggac tcaaggcatt
      661 tacgctatcg agcttgatcc tgccaccggt ttacgcaaag gcgatctcaa cagctacaaa
      721 cgcattaccc gaaacgggcc tcaaaacacc tggggttgga cctcttctta cgaagggccc
      781 gaggtgattt accatcccga actagattat tattacctct ttatagctca cggcgcctta
      841 ggcaacgtct accacacccg ggtggcacga tccaaaaatc ccgacggccc ttactacgac
      901 tactacggaa acaatgtaaa ttatactgtc aacagggata tttatcctct gatctgctat
      961 ccttaccagt ttcagaacca cagcggatgg caggggattt cgcatgtcag cgcctttgca
     1021 tcgggcggag attatttttt gatgaaccag gggcgtccct cctttttgcc caccatgatg
     1081 attatgcata cccgcaaaat cttttggact tccgacggct ggccgaccct cagcccacag
     1141 cgttatacaa ggccgggtat catgcccgcc attacacggg attccatccc cgggaaatgg
     1201 gaagaaatac gactctatga acttatgatg tccaacggcc tggccgaacc cctgggggac
     1261 gacgatgcgc ccgacaagtt cttatgcaaa agcaacacca tgactttcaa cgccgacggc
     1321 cgctttagtt cgaacttcgg ttccgggagc tggaattttg aaaacgacac gctgtacatc
     1381 aagagagata aagaaagcaa acgaattccg ctgatacctt tctatgagct ggattgggaa
     1441 aaccaccgtc ccagcctttt atttaccgga atcaacccca ataacggacg ttcgctctgg
     1501 gccaaaaaag tacctgaaaa cgtcgtctcc tacaattggg taacaaacgg cagtttcgac
     1561 gaaggcctca aagactgggc gctcaacacc tatggcggca gtttcagcga aagcgtggtg
     1621 tccacaacct acggcagcgg aaattcgttt tatgttaaag tcaacagccc ttccgccaat
     1681 tattgggaca gggaactcaa atggagagtc ccggtaagaa ggggtatgcg ctacaaaatc
     1741 agctttctgg ccaaatccaa ttcccaggaa ccctttcata tcgaaatgca acgcctcaac
     1801 ggcagcgctt acaacctgag aaccactttc gtctgcaaca cggccatgcg gacttcgact
     1861 ttcattaccg gggacgccgg agaatcgagc ggaatctacg tgctgaactt tcagtacggt
     1921 actcaccccg caggcactga gttgtggata gacgaagtta aattggaaga actctcgggc
     1981 aaagactacg gcaactacgt caccaacggc gatttcgaaa ccggcacaga gggatggaac
     2041 atgaattatt cggggaacgt ggctgttggt cttgaccggc acaatcccct gagcggccaa
     2101 gcttcgctat acggcaaggt tggcaacgca tccagtctga taagaagtat ggccggttgg
     2161 aaagtttatt tattcaaagg caggcaatac cggctggaat tcgatgcccg cggcagcgaa
     2221 ggcagtgtgc tcagtgtttt cttcgacacg caaaccgcca aaaggataga aaatacactc
     2281 catcccgaaa cccggcatta cagcctgctg ttggacagcc tgagcgaatc gaaagaatac
     2341 acccttaact tttcgttcag taaaaaccag caaggcagcg aatgctggat agacaatatc
     2401 gaactttgtg attacaaaga accggcagca atcgtaaacc ccggtggaca gagtgaaaac
     2461 catgccctgt tgtatcctaa tccggggaag ggcctgtttt acctggacga agcggcagcg
     2521 ccggcaggca gtcgctttta tatatacaac agtgcgggaa tcctgataaa agaactcggt
     2581 ctcagaggca gagagcttga cctgacagag ttgcaggccg gtttatacta cgttaaatgg
     2641 cagggccgtc agcagttgca aaaaattatt attcaataaa tcagcttgaa aacatggata
     2701 aattaaacat cataaaagtg gggggcaaag tagtggaaga ccctgcagcc ctggaacaat
     2761 ttctgcaaga ctttgccaaa atatccggac ataaactgct ggttcacggt ggaggcaccc
     2821 tggctacgga actggctaag caactgggta tagaaaacaa aataaccgag ggccggcgta
     2881 ttaccgatgc cgaaacgctc aagattgtca ccatggtgta cgggggtctg atcaacaaaa
     2941 acattgttgc ctgtttgcaa gccaaaaaca taaacgccct gggactgacc ggagccgacc
     3001 taaacctgat tcgatcggac aaaaggcccg taaaagaaat cgactacggc tacgtgggcg
     3061 atgtaaagga ggtacagggc gacctcttgg ccgaattgtt gaaactgggc attgtgccgg
     3121 tgctggctcc cattacccac gataaaaatg ggcaattact caataccaat gccgacacca
     3181 ttgccgccga gacggctaaa gccctggcgg aatactttga tgttcatctt atttattgct
     3241 tcgaaaaaaa aggagtcctg atggatcccg aggacgataa cagcgtaatt tccgaaatcg
     3301 atgccaactt gttcaaagaa tacgtcgagc agggcattat ccgggaaggt atgatcccca
     3361 aactgcaaaa cgctttcgag gccatccatt gcggagtaaa ggacgtcacc attaccaagg
     3421 ctcaggatat cctgctgggc aaagggaccc gaattatgct atagtatagt ctaatggtga
     3481 tcaggcaaaa ggacgctgat tggccggttg ttgatcgagc tgatctatca gttcttcgtt
     3541 atccatttcc tgattgctca acaccctggg cttgtaaata agcctgcggg gaggaatgcc
     3601 aaaatcgtcg ggataataag tgcttttgcg gttttcacgt ttacgccgct tttcttcgat
     3661 gtcttcttcc agttcccggc tttttatttg cctgtgcacc ttaacctggg gcacgtcgtc
     3721 tatgccaaag ccactggcca gcatggtcac ttttatttga tcgtccaagt cgttgtcggc
     3781 agcgattccc cagatgactt cttccacctg gttgacaaac tgatccatga acactctgat
     3841 ttgctccatt tcatgagtca tgatctccga cttatcgctg caggtaatgt tgaacaatat
     3901 cttgcgggca cgaaatacat cgttgttatt gagtaaagga gatttcaagg ccgaattcaa
     3961 agcctcggtc agacggtttt gtccctttcc gtaaccggca ctcatcactg ccactccacc
     4021 gtctttcaga atcgtattca cgtcggcaaa atcgagattg atatagcccg gcaccgtaat
     4081 gatttcggca atgcttttgg cggctatcgt caaagtatcg tctgccttga gaaaggcgtt
     4141 tttgaccgtc aaatcgggat agatgcgaaa aagttgctca ttgtttatca ccagcaaggc
     4201 gtccacattt ttgctcattt cctccacgcc gtccagagcc tgatttattt tagattctcc
     4261 ttcccagcga aaaggaatgg taacaattcc aacggtgagt atgtccattt cgcgggctat
     4321 accggcaata acaggcgctg ctcccgtccc cgtgcctccg cccattccgg cggttacaaa
     4381 taccatgcgg gtatgttcgc caaaagctgc cttgatatct tccatacttt cttcggcagc
     4441 cgctttggca atttcgggat gattaccggc acccaaacct ttggtagttt ttgggcccaa
     4501 ctggagttta tcgggtatgg cagaattcag catggcttgc ttgtcggtat tgcatatcag
     4561 gtagtttacg tctcgaattc ctttacgaaa catattggtg acagcattac cacctcctcc
     4621 gcccactcct atcactttaa taatagccgg ttcaccggag ggtaaatcaa agggtattaa
     4681 ttcatctatc atactctatt tcaataatct ccttgtttca ttaagtcatc aaaaatatta
     4741 ccgatcttgc ttatcagctt ctctttattc tttttatccg gctttgccgc ttcggttttg
     4801 ggcctttttt ggggtgcttt ttgagtgatt tcttccaatt cttccatggg taaagttgcc
     4861 tgcctgggtt gttctttact ctgcccggaa tgtacggggc cacggcaacc acccttctcc
     4921 ccgagcatga tcaaacctat ggtttgtgcg tatgccggat cctgaaattc cacacgacag
     4981 gaagaggata aacgacagga caaatcggct ttacgtacgg gcatttgtgt ttttatccgg
     5041 agtaaatcct ccaatccgtc catttttgat gctcccccgc atataataat accggatccg
     5101 attttgccga cttctactcc tgaataaata atttgagccc aaatattttc aacaatctct
     5161 tcgctgcggg ctaccacaat ttcgttgatt tctttatcgt cgtgtaaagg ttcgctgaaa
     5221 aagcgtattt cttcttccgt tttgttgaga gcctcctttt tcataagctc ggtataatgc
     5281 aaggctgttc cctcttccaa tttcagcgac tcggcctggg aaaaactcag gttaagttgc
     5341 ttcaaatcgc ggctgatgtt tttacctcca taaggaataa cacaaacata acgaagtaca
     5401 ttatccttat aaatacacat cgaggtggta cctcctccaa aatcaatcac gacagctccc
     5461 ttatttttgt cttcttcgct caggactgct tcggcgctgg cctgtatacc cagaatattt
     5521 cggcaatctt tcaaacccgc gttgctcagg ctattttcaa ggtttttcat gtactgggtc
     5581 ctggctgcaa tcagcttata tcggccttcg agcgaatcgg gacgctctcc tttcggattg
     5641 aggcagggaa aaccgtccac caggtattct tgttcaaagt gggcaatcat ctccttgttt
     5701 tcgggcagag atctggctat ggcgtcatcg aaaagctccg aaagtatttc ttcggtaatc
     5761 agttcctgtg aattgaggtt gagtttttca ctactatcga tgcattgcgt actgtaaaga
     5821 ttcaatccca catacacccg gtcaatgctg caaccgcggt ccagatctct gagtaattcc
     5881 cgcatcaatt cttctaccat caggcctacc tgatccacat tctgcactac accccgacga
     5941 atctcagtat caataggcct ggacttcgaa gacacaatgt tcactttccc catatcgtcc
     6001 ttatacccca ggctaagtat caaattggag cttccggggt ccagtacggc tatgtacgat
     6061 tgaacgttat tcatatgttt atttttttgt acaaactatc tgattctcaa attcagtgct
     6121 gatgcaacgg tatctatccc aacccaacct gggtaaagct tcttcgtaaa agcaacgcaa
     6181 cgcccttagc ttatattcca tattttctgc tttgcccaac ctgatctcaa gctctcctaa
     6241 tctgggaata aggctgagtt tacccgaaga ttcctgcaca atctgtgcaa tcatggcatc
     6301 ccaaaaatcg tctttgttta tcaggcgagc caattcaaac aaaccggcca gactcaaaga
     6361 atctgttttc tgcaccaaag ctaccggcac atccaccggt actcttttgt tgattttcat
     6421 ctttttgccc tcttcgtcca gaaaaaacgc ttctccacct ccggtataca ccctcaggac
     6481 gggaatacgc tgatcaatct taatacacaa gcagccggaa ggcgaaagaa agcattcggc
     6541 tttttttacc atagaatgat tatctatcac cctttctatg cgactcaggt ctacctctat
     6601 taacttcttg tcaattacct gcagttgatt ttctttcaac aggcggccaa tttcttttga
     6661 tgtaacataa ggatggtcca cactgtcttt cactttgata caaacccttt gaacctgcag
     6721 ctcgttttgt tgcctgctgt agagcctcac ggaaaacagg acatagcctg ccatcaacac
     6781 ggagcctatc catataaaca cccttccggc catcaggaat acttctcttt taataatggc
     6841 accaaacggt caatacttcc ggcccccaaa gttacaagaa cttccggttt tttcctatcc
     6901 agcagactta agatttcgtc catggaacaa agggttttcc cttcgatggt cattttgtca
     6961 aatataagtt tggaatccac tcccggaata ggctcctccc tggccggata aatatccagc
     7021 aggtaaacat agtccaaacc cgacagggct tgagcaaatt cgtcggcaaa gtcacgcgtc
     7081 cggctgtaaa gatggggctg aaaaataccg gtcagttctt tagtaggata tagtgctttg
     7141 agcgagcgga tacaggcttt gagttcattg ggatgatgcg cgtaatcgtc gatataaaca
     7201 aaatcgttgc ggcgaatctg aaaatcgaaa cgacgttgat tgcctttgaa cgaagccaaa
     7261 gccggtcgca attcttcggc actcactccg cagaggtagg ccaggcccat ggccgccaga
     7321 gcgttctcca catgtatcgt aatcggcagg cctaattcca tttttttcaa cacacccccc
     7381 ggataaataa aatcaaaata caaacggcct tcttctatcg taatgttttc tgcccgaaaa
     7441 tcggccttgc tgctatgagg catttgctcc gtagcgtaag tgtacgtttt tacaccctcg
     7501 gccaaacgag gctgcaaaac cagactctgc ttcatgagca agttcccgcc cggacggatc
     7561 aaagaagtaa aatgggcaaa agcctccagg taagcttcgt ggctgtggta aatatccaga
     7621 tgatcgggat ctaccgaagt aatcacagcc atccaggggc tgagccggtg aaaagacctg
     7681 tcgtattcat cggcttctgc caccataagc cggcttttac cggagagcaa aaggttggta
     7741 ccgtagttga gggatattcc gcccaaaaag gcattgcaat cgagcgaaga ttgtctgagc
     7801 aaatgggcca gcaacgtact ggtggtggtt ttgccgtgag ttccggccac acacagggcg
     7861 tcgagttccc gggaaatctc acccagcact tcggcacgct tcacgattct gaagccggca
     7921 gaacggaaat actgcaattc ggaatgcata tcggaaacag caggtgttct cactaccaag
     7981 gtggtattca cctctttgaa agcttcggga atggcctcca cgtcgtcgca gtaatgtatt
     8041 tccatgcctt cttttaccag ttgtttggtc aattcggttt ccgttctgtc gtatccggcc
     8101 acacggcaag aacgggaatg gtaataacgg gcaagattgc tcatgcctat accgccaatc
     8161 ccgataaagt atacctgctt aattgtcata tcttcgctaa tttcattatt tcatctgcta
     8221 tgtgttcggc agcatgcggc aaagccattt ttttgatggc gcttctcagt tcttgcaagc
     8281 gtccgggctg ttccaacagg gccaaagcct cgggaatgag tttttcgcag gcttcctcat
     8341 cgccgatcat aacggcggct cccttgcgca ccaaggccag ggcattttgc gtctgatgat
     8401 cttccgccac attgggcgaa ggaaccagca aaaccgcctt acccaacagg gcaaactccg
     8461 aaatggaacc ggcacccgcc ctcgacacga ccaaatcggc cagggcatac gcatagtcca
     8521 tcctcgaaat aaagtccgtg attaggatag ccttattgtt gagttctgca ttagcagcca
     8581 taatttcttc gtaataatac ttgccggttt gccagatcaa ttgcactccc gatttttcta
     8641 tggattcaag ttgttgctgc actgcccggt tgatactgcg agcgcccagg ctgcctccca
     8701 ggacaagcaa aacagggcga tccggcttca gcccgaaatg cttcagggcc gcttcctttt
     8761 gattgagtga acgaagtaaa tcctgacgca cgggattgcc ggtaagcaaa atcttttctt
     8821 tcgggaaaaa gcgctccatg ccctcgtaag ccacacaaat acgggcagcc cctgctgcaa
     8881 gtaatttatt ggtaattccg gcatacgaat tttgttcctg tatgagacaa ggtatttttt
     8941 tggcagccgc ggccctgagc aaaggaccgc tggcataacc gcccactccg accgctacgt
     9001 cgggcttaaa ttcgcgtatc aacttgtggg ctttacacat acttctcagc aactgaaaca
     9061 aggttttgac attactcagc aaatgttgac gatcaaaacc tctcacaggg agtccgatga
     9121 taggatagcc tgctgccggc actttttcca tttccatgcg gttttcggcg ccgacaaaaa
     9181 gtatgtcggc ctcggggcac ttttgcctga gggcgttggc tatggaaatg gccggaaaaa
     9241 tatgccctcc ggtacctcct cccgaaataa taaaacgcgg tttgcgcgtt tcgttattct
     9301 gctgcttcga tttcttcctg ctgttcataa tccggatctt ccgtttcgtt cgatactaag
     9361 ttttcctctt caggtatagg tttttgtgtg gcaaaattgc taacactcag tataatagca
     9421 aaatacaaac acgtaatgac tcccgaactg cctcctctgc ttatcaaagg caaaggttgt
     9481 ccggtaaccg ggaaccagtt taccgccact gccatgttga caaaagcctg catagatata
     9541 atcagtgccg aaccaagcac caggagcata ggcactgatt ttgtacaacg tttggcaatt
     9601 tttaaggccc taaacaataa aaacaaatac aagaaaggca ccccaaacaa acccagccag
     9661 cccaaatcct ctatgataat ggcaaaaata aaatcggaat aggcttgagg caaaaagtct
     9721 cgctgactac tgttgccggg taaaacaccc aaaaacttac cctcggaaat agctattttg
     9781 gcatgagaaa cctgccggtt ctcgtccgta attttaaatc cttcatccct gacgtccagc
     9841 tgttcggtca ccaatctgtt gtgccaggtt tcccagcggg gcactccgtc caatcgctct
     9901 accttgatca ataaaaacaa cagcaaagcg gctgccgaag ccaccagtaa aaacaaaatc
     9961 aggagttttt tccagtccac cccagccacc accatcataa gaaaaacaaa gaggcagagc
    10021 aaaatagcag tggacaaatt ctcaaacacg atcaaaaaag ccaaggcggc cgtcacaata
    10081 agtaccaccc aaaatgcctt atccatggcc tctttagagc cctgagccct gcccagaaac
    10141 caggcagaaa acaacaccat cgacaatttg gccagttccg agggttgaat ctgtatgccg
    10201 aaaatctcta tccaacgcgc tgcttcgttt agcctgacac ctattatggg agtcagaata
    10261 agcataataa ccgacaacaa tagtaggggg aacaccagca gagcggtata cttgtaatgg
    10321 aagttatgaa aagccaccac caaggcaacc cctatcagca aatataaacc atgtcttata
    10381 ataggctcga aaggattcat tcgggcataa gccagggttc cgatggcgct aaaaacctcc
    10441 accaacgaaa ttatgcataa aaacaaaaag atcatccaga tcactttgtc gcctttgaac
    10501 acactttgta tcagttccat ctcttataaa ttgcgaacgc agtccttgaa ttgacgaccg
    10561 cggtcttcgt aattttcgaa aaggtcgaaa ctggcgcagc aaggcgacaa caagacggta
    10621 tcgcctttgg ttgccaggct gcttgccaga tccactgcct ctttcatcga ccgtgcatcg
    10681 cgaatttcgt ctactttacc ctcaaagaaa tcgtgcagct tagtattgtc aacacccagg
    10741 caaacgatgg ctttcacctt ttgcctgacc aaagcttcaa tttcgccgta gtcgtttcct
    10801 ttgtcggtac ctcccattat caacactacc ggtgtattga ccgattcgag ggcgtaccag
    10861 caggaattta cgttggtggc tttggagtcg ttgatgtatt ccaccccggc caccccggcc
    10921 actttttcca aacgatgttc cacacctttg aaattactca gggccgtgcg tatttcttct
    10981 ttgcgaatat gcgctatgtt ggccgaaata gccgacgcca tagtgttgta aaggttatgg
    11041 gttcctcgca aggccaaagc ctcctgttct atttcaaatt gctgatccgg agtattgata
    11101 accagtattt tatcttcgat aaatgctttt atgccggcat cttttttgag ggagaaaggg
    11161 taggttttgg ccctgattgt tcttttcttt atctcccgtt gaataatggg gtcgtcgttc
    11221 cagtaaataa aagcatcctg ctcggtctga ttctgaataa tccggaattt ggagtccacg
    11281 tattcctgca aattataatg ataacgatcc agatggtcgg gagtgatgtt gagcattaca
    11341 gccacatccg ccttgaattt aaacatatag tccaactgga aactgctgag ttcaatcaca
    11401 tagtaatcgt agtcctgctg agctacctgc aaggcgaaac tttgacctat gttgccggcc
    11461 agccccacat tgagccctcc gtttttcaga atatgataag taagcagggt ggtggtggtt
    11521 ttaccgttac tccctgtaat gcaaatcatt ttggctttgg tatagcgccc ggcaaattca
    11581 atctccgaaa caacgggtat acccttggct ttgattgttt gaatcagggg agctttgtcg
    11641 ggaactcccg ggctcttaat gacttcgtcg gcgttcaaaa tgagttcgag gctgtgttgt
    11701 ccttcttcga atgctatcat gttatcctcg agcaatttac ggtaacgctg gggaataggg
    11761 gcattgtccg tcaaaaagac atcgaaaccc tgtttcctgg ccaatactgc cgctccgctg
    11821 ccgctttcgg ctccccctat aacaacgatt ctcttcattc ttacctgatt tttagtgtta
    11881 ttatggccaa agcagccaac aacagactga tgatccaaaa acgaaccaca atcttggatt
    11941 caggtatggc tttgatgggt ttttgaatca aagcctcgat gcccgcattg cccggctttt
    12001 gaaaatgatg gtgcaaagga gccattttaa aaatgcgctt ccccacccca taacgttttt
    12061 tggtccattt aaaataggag acttgcatca tcaccgacaa ggattccagc aaaaacacac
    12121 cacagaaaaa caacaacatc aattctttgc ggatgataat ggcaaaaacg gcaataacac
    12181 cgcccaacat cagactgccg gtatcgccca taaatacttg cgccggaaaa gaattgaacc
    12241 acaaaaatcc cacagtggcc cctacaaaag ccgacatata aatgaccaat tcctcggaac
    12301 cgggtatgta cataatattc agatacgaag caaagttcat attactcgat acataagcca
    12361 atatacccaa acccaccccg ataatggccg aagttcctgt agtaaggcca tccaaaccat
    12421 ccgtcagatt ggctccgttg gaaacggcgg ttacgataaa aatggtaacc agcacaaaca
    12481 gcacccagcc ggctgcctgc ttgtgttctt tcataaaaca aaccaaattg gcataatcga
    12541 ggttgttgtt tttaaaaaaa ggaatggttg tcttggtcga ttttacttca ccttcttcaa
    12601 aacggacttc ttcgttgggc aggccgttgt tgagctgaac attttcgcga atcaccactt
    12661 cgggactcag ataaagggtc agccccacaa ttaatcctat gcccacctgg ccgataattt
    12721 tgaatttacc cttgagccct tctctgtgtt ttttgaacac tttgatgtaa tcgtcggcaa
    12781 aacccaataa ggtaagccaa acggtactga tcagcatcaa aatgatgtaa atgttgtcca
    12841 gtttggcaaa aagcaatacc gggacaatga gtgaaagaaa aatgatgatt ccacccatag
    12901 taggagtgcc ttttttactt aattgaccct ccacgtccag atcgcgggga acatcgccta
    12961 tctgcatacg ttgcagttta cggattattt tccggccgat aaaagtggct ataagcagcg
    13021 aaaagataaa ggccataatt gacctgaacg atacgtactg aaacattcct gctcccgtaa
    13081 agtcgatact gtttaaataa tcaaacaaag gcactaacat attctcttgt tttgtggctg
    13141 taaactttta taaataaaac aacaaccggg tattggtatc agagtaattc tttaaaaatt
    13201 tcagtaatta tttttttgtc gtcaaaagga tatttcactc ctttgatttc ctgataatct
    13261 tcgtgtcctt tgccggccac caacacgtaa tcgcctttac cggccagtct gcaggcagta
    13321 cgaatagcct ccctgcgatc gacgatcgac agcactcttt ttcgtgcttc ggcaggaaca
    13381 ccggcataca tatcgtccaa aatagcctgc ggttcttcaa acctcggatt atcggaagta
    13441 agtatcaggg tatcgctggc ccgcacagcc tccagagcca tttcgggacg tttcgtttta
    13501 tcgcggttgc cccccgctcc caccacactg ataatcttgc cctgcttgcg tatctgagcc
    13561 agggtttgta aaacgttctt cagagcatca ggtgtatggg cgtaatcaac tatagcggta
    13621 taaccggtgg gagagttcaa aaattccagc ctgcccgata ccggacgcaa ggtgcttatg
    13681 attctgagca cttcggcggc atccatcccc aacaagacgg cagtcccata tacggccagt
    13741 aaattcgata cattgaattt tccgataaaa cgaacgtgta cctcccgacc gtcaaaatcc
    13801 agttgcatac cgtcggaatg agtttcgagc aatctggccc tgaaatcggc caaactgcgg
    13861 caggagtagg tataatgggc agcccggcaa ttttgaagca tcacttttcc gtttttatcg
    13921 tccgcgttgg tcaaggcaaa agcctctgcc ggtaaacggt cgaaaaagct ttttttggcc
    13981 ctgagatagg cctctaccgt tttgtggtag tctaaatggt cgtgagtgag attggtataa
    14041 attcctccgg caaagtgcag accggctatc ctttcctgat ctaccgcgtg tgaactcact
    14101 tccataaaag catattcgca gccggccgcc agcatctgcg ataacaaact gttgatggca
    14161 taagcatcgg gggtggtatg agtagccggc aattcctgtt catccacgcg aacgcaaacg
    14221 gtagacaata aaccgacttt ataacccaga gctttgaggg tattgtagag caagctggct
    14281 atggtagtct tgccgttggt gccggtaact cccaccaatt tcagcttacg cgagggatga
    14341 gagaaccagg cggcagatat taaacccaag gccagattgc tgttgcctgt gcggatatac
    14401 gtcacctccg gatttatctc cgaaggcagg ttttcgcata ctatcactgc ggcccctttt
    14461 gccaccactt cgggaatata gtcgtgcccg tcgctgcggt tgcctttttg agctacaaaa
    14521 agacaagagg gagctgccag ccgggaatca aaacaaacgg agttgatctc cttgtctttg
    14581 gggcccaaat gctccaaaac agtcaaatct tctatgcacc gactcagttt catgcttatc
    14641 gtcaattcaa acgtaatacg atttcctgcc cttcgtgcga gacggtaccc ggacggagcg
    14701 attgttgcac caccttgcct ttgcccgtaa gcctgacttg cagaccccat tgctcgagca
    14761 acaaaatggc atccctggct cccatgccca acacgttggg caccaggttt tcaataaggt
    14821 ttctttcttc gaggagtagt cttttacttt cttcctcccg cctggccact ataggacgtt
    14881 caccatggac tccggaatcg tcgtatttta tattcaattt gtccataaca taaaagctgg
    14941 ggccgggcaa acctgtttta attttgggag gaccggtctc tttggtctct ttgacccgtt
    15001 tactgttcag gtctatccac aattcccggg cataaatctg ctcggcaatt tccttgaaaa
    15061 ctcctccggc catcacacct cccgaggcat agcccactac cggatcgcgt atcaccacaa
    15121 tggcgctgta tttgggacga tccgaaggga aatagccaca aaacgaaacc tgatagtttt
    15181 tgcctccttc cttatagccc atggaacctt tggacaaaac cgccgttcct gttttgcctg
    15241 ctatggggaa aaacttactt ttcaccgctt ctcccgttcc tttttccacc acgctgtcca
    15301 acattgccct gatttcgcgc aaggttttgt cggagcatat ttttaaattc aggacctccg
    15361 taccaaagct tttatgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnattt
    15481 tgccttcttc cagggcggcc atcatggcta tggttttgaa ggtggaaccc ggctcactga
    15541 ggtcgcttac ggcgtagttt tgtatttcct ggtaagatcc cgtggaagta aggcctaaat
    15601 tgctgattgc ttttactttg cccgttttta cctccatcag tacggcggtt cccgaggctg
    15661 ctcccgtctt ttggagcatt tccttcaaag ccgtctcaac gatgtcctgc atgggaatgt
    15721 tgatggtcgt ccttaaattc caaccgtccc ggggctcttt gttgttgaca ttcatgtatt
    15781 ggccggccac tttttgacgg gtcgacactc cgtcctggcc ccggagcaaa gaatcgtact
    15841 ttaactcaag cccactgcgc ccgcctttgc cgaattcggc gtaaatattt cctatggttc
    15901 tcgaagccag agagccaaac attttttgac gttccaggta ttttttttca tacaatccgc
    15961 tttgataacg gcctttacta aacaaaggga aggtcttgat ctctttccag tcggcgtaag
    16021 acactctccg cggatgtatc agatactcac gactggccct gcgctgcccc tcgcgcagcc
    16081 ttgcttcaaa atctgccggc gacaggtcgc cgaatttttg cgacagagcc cggcaaagcg
    16141 gttttatgta tttattgagg gtatccctgt gtacggcctg aaaatccata tacagggcat
    16201 agctcggcac tgtggcggcc atcagcaaac cctcgtccga aaaaatattc cctctgccgg
    16261 cgggaacaac aacgttttcg cgctgttgtt tgtttttcca ttctgcccag gccgcccctt
    16321 cggcaaattg tatatgaaaa gctgcaaaaa tgataccaaa cagcatgaca ctaaaacaca
    16381 gaatcactat gatcaaatag cgtttgcgta taccttttgt tatgtcttca cctgtctgtt
    16441 cgtccatttc ctttccaagc ctgcaagcgg ctccatttat tttactttca aggtataggg
    16501 aggctcggtt aattccttta attccaagcc tttttcctct accatcgatt taatatgcga
    16561 ttggcggctt tcctgcataa actccgaggt gtacatcaaa gcctcgtatt ttacatcgtt
    16621 aagttcttgt tgcaacctgt ttatttcggc cagtttcatt attaccgagt aatgattgcc
    16681 tatataaaca aagagcaaca ctactataag gaatatcaat ttggcgtgtt tgcgaaagaa
    16741 attcttatcc agaaagcttc cgtctataaa ttcgccaaaa ataaaacgtt tggtcgtttt
    16801 tttattttct tcttcctgtt ccatttctta acgcttttct gctattctaa ggcgggcact
    16861 gcgcgaacgc ggattcagtt cctgttcttc cggcgaaggc accagcacct tgttgttgat
    16921 cagaataaag ggactgagat tgttgccgta aaaatctttt tttacctcac cctccagatt
    16981 tccggtcctc ataaaatttt tgaccaaacg gtcttcgagc gaatgataac tgattaccac
    17041 caatcgacct ccgggcttca agatatccag ggattgtgtc agcaaatcct ccaaggctcc
    17101 gacttcctga ttcagctcaa tacgcaaagc ctgaaaaact ctggccaggt cttttttttc
    17161 ggcttgcctg ctcaacaaag gccgcagcaa atccgacagc tgacctgccg ttcttacagc
    17221 ttgttcctct ctagccttca cgatagcccc ggccatcctt gcagcctgac gaaattctcc
    17281 gtacacgcga aatactctac aaagctcttc tttggaataa tctgccagaa tatctgcagc
    17341 cgtaagagta gctctttgat tcatccgcat gtcgagatcg acatcaaaac gaaaagagaa
    17401 tcccctgccg gcctgatcga aatgatgaga cgatacgccc aaatctgcca aaattccgtc
    17461 cacttgttcc tctccgtaat aacgcataaa attggataaa aagcgaaaat tgccgtgaac
    17521 aaactgcaaa cgtttatctt ccggcaaatt tttcagtact tcttcatcct ggtcaaaggc
    17581 atatagccga cctcccttaa gctgcgccaa aatggcgcgc gaatgacctc ctcccccaaa
    17641 agttgcatcc acatagatgc ctcccggttt cagagccagg ccatctacgc tttcactcaa
    17701 caaaacagga atatggtagg gtgtttccat ggtgtaatta ctccgggatt ttggttttgg
    17761 tcattagttc ctgcatccgg gttttaaaca gctccggatc cactcttggc tgctcgagag
    17821 cttcttttgc ccagatttcc atggtattat ccacgcctaa aaaacgtaca tttttttcta
    17881 taccggtttt taacaacaga cgtttggaaa tcaatatacg tccgttagga tccatttcta
    17941 cggcctcggc ttccagcata aactgccggt aaagttcctg ctcctgagga ttccatttat
    18001 taagccgggc acgcaattcg ctcaattctt cttcccatac cggagccgga taaaccacta
    18061 tgcaattctg gtaaacatct ttgcgcaaat acaaaattct actttgcgag ccttcatcgg
    18121 ttttagtaaa aacggcgtta tccagcactt tcctaaaggc ggcaggaaca aacactctgt
    18181 ttttggcatc cagtttagct tcgatatttc ctaaaaatcg catgtttctg tctttttaca
    18241 gcctatattt tgactatttt accccataga ggcaaatttt tcagacaaaa ataaacattt
    18301 tcacccactt tgctacattt tcacccactt tttttaaata aaatgagata aaaagttttc
    18361 aacaagtttt caacagccta tatagctgat tatctgtaat ataaacttca tggatatttt
    18421 tctctttctt ctcttgtttt taaaattctc tgtgttttta tggaaaacaa ggccaacaaa
    18481 ggtcagaatc agagcaatca taaaaaaact acccactctt cggctttata attatgtctt
    18541 agctttacaa acctcacaca cggaaatact ttgaggaacc cgatacccac catcgaagac
    18601 ggtaaataaa ccgggaaaca aagagaaaaa acagagtcgt aaaaaagccc tgctaattcc
    18661 ccgataaatt tgtacttttg cggcttaaaa gcattcatac ttatgacccg acaattcaaa
    18721 cggaccaccg ttaccaccgc tctgccctat gccaacggac cggttcacat cggacacctg
    18781 gccggcgttt acgtcccggc agacatttat gttcgttatc tcagactcaa aggacaagac
    18841 gtcttgttca tcgggggctc cgacgagcac ggtgtgccca ttacactcaa agcaaaaaag
    18901 gaaggagtca gccctcaggc tattgtagac cgctaccacc acatcatcaa gaaatccttc
    18961 gaagattttg gtatttcttt cgatatttac tcacgtacca cttcggccat ccatcacaaa
    19021 accgcttccg ccttttttga caaactcaac cgccagggca gctttgtcga aaaaaccagt
    19081 gaacaatatt acgacgagga agcaggccag tttctggcgg accgctatat caccggaacc
    19141 tgtccgcatt gcggcaacga aaacgcctac ggcgaccaat gcgaatcctg cggaaactcc
    19201 ctcagcccca acgaactgat acatccgcgt tcgaccatca gcgggagcaa acccgttatg
    19261 aaggcgacca aacactggta tctgcccctg gaccaatacg aggcatttct gaaacaatgg
    19321 atactcgaag agcataaaga atggaaaagc aatgtctacg gacaatgcaa atcgtggctc
    19381 gacatgggcc tgcaagccag ggcggtgagc cgcgacctgg actggggcgt tcccgttccg
    19441 gtcgaaggcg ccgaaggcaa agtgctctac gtgtggttcg acgcacctat cggttatatt
    19501 tcaaatacca tagagctctt gcccgacagc tgggaaaaat ggtggaaaga ccccgaaacc
    19561 aggctgatcc attttatagg caaagacaac atcgtgtttc actgcatcgt gtttccggcc
    19621 atgctcaaag ccgaaggcag ttttattttg cccgacaacg tgccggccaa tgaattttta
    19681 aacctcgaag acgacaaaat atccacttcg caaaactggg cagtatggct gcacgaatac
    19741 ctcgaagact tcccgggtaa acaagatgta ttgcgttatg ttttaactgc caacgccccc
    19801 gaaaccaagg acaataattt tacctggaaa gattttcagg cccgcaacaa caacgaattg
    19861 gtagccgtat tcggcaactt tgtcaaccgg gctatggtgc tcactcataa gtattttcag
    19921 gggaaagtac ctgctcgggg cgaacctaac gattacgacc ggcaaacaat ggaggagttt
    19981 accgacgtcc aaaacaaggt cgaaaccctg ctcgatgtat tccgctttag agatgcccaa
    20041 aaagaagcca tgaatctggc ccgcatcggg aataaatacc tggccgacac cgaaccctgg
    20101 aaactggcca aaacagatct gccccgggtg ggtagcattc tgaatctctg cctgcagata
    20161 gccgccaacc tggccatcgt ttttgaaccc ttcctgcctt tcacggccca aaaattgcgt
    20221 aggatgctgg gcatagacag cttccgctgg gagtcgctgg gcggtaccga tctgctgcct
    20281 gtggggcatc agctcaatcc cgccaaattg ctctttgaaa agatagaaga cgatgccata
    20341 aatcaacaga ttcaaaaact gctcaacacc aaaaacaaca ataaagccac acgggaaaac
    20401 caagcagaag caagctggac ccctgccccg gccaaagttc ccatcgatta cgacagtttc
    20461 tgcaaaatgg acattcgcgt tggtaccata ctgcaggccg aagctgttcc caacaccgaa
    20521 aaacttatga agatgctggt agacacgggc ctggatcaac gcactattgt ttcgggcgta
    20581 ctggaacatt tcagtcccga ggagcttatc ggtaaacaag ttccggtcct gatcaatctt
    20641 gaaccgcgaa aaatcagggg catcgtttct cagggcatga tactctacgc cgaaaacgac
    20701 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaaatgaaa gaccaattca
    20821 atatttattc ttccgactct caagccagca aagttctgcc cttaagtcct gaagaatttg
    20881 attttgaagc ctatgccgaa tacgaagccc gcttaaacca aggctgtaag aagttttggg
    20941 aagctccctc cggtgtaatg gtctatcgcc gtatgcgggt aaaagaagtc tttgccgaag
    21001 acagtgccga ttggcagaaa tcccttaatt ggcagttggg agccctgcaa caaagtatgg
    21061 gctataaagc ggacattccc aattttctgg agccctggta cggtataggc accatagcct
    21121 cggcattcgg tttcggctat cgctgggaac aaggccaggc gccggccctg gacggtaaat
    21181 ttgagcagtt ggaacatttg ctgcaggccg atttcaaacc ggtagcccaa acagccatcg
    21241 gcagtcacag cctcgaaatg accgaatact ttctggagca gacccggggg cgtttgccca
    21301 tgtcgtggtg cgatgtccag tctcccctga atatcctgtg caacctggtc gattccaata
    21361 tgttttttcc ggaattttac ctgaatcccg aagcggtaga acaggcctta gataagatca
    21421 ccgatttact catcgagttc accaaggtac agaccagact cataggcccc gctttggtca
    21481 agcccggaca cggttttgct tcgagcaggt tttttgatgg ctttggcatg agcgacgaca
    21541 atatcgttat gttgcctccc gacctttaca acgacttcgc cgtaccgaga atggtccgtg
    21601 ccggccggcc ttttggaggt ccggtgttcc actcctgcgg caactggtca gacaaaaaag
    21661 aagacattgt ccgtatagac gggctccgaa tggccgacgg tgctttctcc aaggctaccg
    21721 accccgatgc caatccctgc gaaggttttg ccgccgcctt tgctcatacg ggcattgttc
    21781 tgaatgcccg catagtcggc gacctcgaaa ccctgggcca aaaggtgaaa gaactttggg
    21841 ctccgggcat gaaactcatc gtggtcactt attgcgaaaa tccctcggaa caggctgcag
    21901 cctacgattt aatccacgag atttgccggg aataaacgat actgttcgtg ccgggcgaac
    21961 caaaaaaagg ggatgtctct tagagacacc ccctttttgg atataagcat cattagttat
    22021 tcctatcggg cataaataat gatctttttg acttcaatgg caccgtcttc gtaccggatg
    22081 cgttcaatat tgattcccac accgggttcg agcaacctgg tgccgctcaa atcgaagtat
    22141 tcgcgcgaaa cgactaccct gtcttgagaa ataccgttca aagcttgttc tttttccacc
    22201 ttgatttcat cagtggtggc cagcgtagca gagtcggcca taatacaagc ctggaaggcc
    22261 ggtattaccg gattggctgt tttcacaaaa gccgtaccct cgtcgttcat cacgtaagcg
    22321 taaacggtgg tagtgtctga accaagagca tgaggtataa agcgtctgtt ggttctgtaa
    22381 gctatgccac tcgacggagc gttgaaagtg gtctgagcaa tacccagttc gaaacctgtt
    22441 tcgctctcgt atacgaaagt tataggctga cctacatact tttcggccac tttgattaca
    22501 tagcccaccg tgttggcaac gatattggcg gtcggtccca gttcaacaaa ttctcccgag
    22561 gagagtcgtc ttataacgta gtcggttccg cgtaccagta cattgctgtc ggcatccatg
    22621 ataacaggct tgttgaaagg cgctacaaaa ggttcgtaac cggagccgaa tacacggcta
    22681 aagttgacgg ttccagttat agacagcact tcattctgag cgatgttcat agccttgtcg
    22741 tattccacat tgacatttcc ggcagcagta atatctatac ttaccgtatt tacatccaaa
    22801 taaatgtccg gaataaagac attcaccttc tgcaaggttt tataacggtc catagtatag
    22861 ctgcaagagg tcgaagtgga tttcactatg taggatacat tttcaataat gaactctaca
    22921 aactggcctg cagtaatatt acttacagta actgccctgc ctccgctgga ctggctgtac
    22981 aaaccggcaa gtttaccatc agctcccgta cggagttcgt agctgtcaaa gttcgctgcg
    23041 aaatcgggga agtactgaat cttgaccgaa tcctgatcgt aatagaaaag gttatcggcg
    23101 ttcttatcac tgcctgcccc ttcgagcaga tgaatattga tcaacacatt cagtgaatcg
    23161 taagaggcgg tccagccgtg agccaacatg gtatctataa cggcctgatc tacctgagta
    23221 aagtcgtaag acttggctac ccggtaattg gtacggccgt ctatagctat gctggtactg
    23281 gcagaaacat aaccctctgt cgacgcggta gcagtcagtg tgcagctttc gtctatggtc
    23341 aacggacctg agtacacacc gctgtttgtt ccgtattcgt aattaatcac cgaaaccgga
    23401 gcaaacaaca catcgctgtt atcactggta atggcataag tcttggctgt tccattcact
    23461 gccaccaacg aaataacagg tgcattgacg gcaatttcgg tgccggctgc atatacgccc
    23521 ttcaccagtg ctgagttgac agagccatcg gttgcataag ccaacagata agaggcttca
    23581 gcgtcgacca tgaagggggc ggtataagtt tgccattgat acttggtagt gtcaacatcg
    23641 gcaatttcaa cagccgcttc gtagtcttcg gcactgacaa ggacatactt caagatggca
    23701 gtgggctgcg tagaaagcat ggtaatgata cgggatattc cgctcactcc tgtcatcaaa
    23761 acggtaggtt ctaccataac gacacccttg accagtttgc tgaaagcgat gttatcgatc
    23821 ttcatttcag atagataacg tcctgccata taatacagac ccacgcattc gatgcctgca
    23881 ctgcccgaag taatggcagc tttgtcgaga acggcggagt cggcagcatt ggtaatcgtc
    23941 agcaatatgc catcggcact tatttcggcg ctatagtggt agaattcgag tgcattcagt
    24001 tccacagtat ggctttcggc accactgccg gcaatggtcc aggtagtaga attggtggtg
    24061 gacaatttaa agagataacc tcctcctgca ccccagttga cattatcacc ggtgtaggca
    24121 aaatcagttc ccttaactgc aaattcggtt gcctggttat taccggcttt cagtgccagg
    24181 tcaaattcca gaatgtactt atctacatcg gtaaagtcta tctcaaagtt agaataacta
    24241 cctctggaat tgacgctgcc gtggttgtaa taaatgtatt tattaccttc ttcttcgacc
    24301 agggaataac cggcctggtt gttgggagaa gtccatacat ctgcggcaga agcaacgttt
    24361 tcgtaatcct gcatatacca ggttaccttg gtcagcggca caggtttact cagcaagata
    24421 ttatctatct tcatcttcga ttgataacga cctgcctgat aatgcaggcc tacgcattcg
    24481 ataccttcac tgcccgaagt aatggcaaca ttatccagaa caacggaatc ggcagcattg
    24541 gtaatcgtta gctgaattcc atcggctctg accaaagcca ggaaatgata gaattcaagc
    24601 gcattcagtt ccaccgtata gctttcggca ccactaccga caatggtcca ggtggtagaa
    24661 ttggtggtgg acaatttaaa gagataccct ttttctgcac cccagttgac attaccaccg
    24721 gcgtaggcaa aatccgttcc cttaagtgca aattcggttg cctgattatt tccggctgtc
    24781 aatgccagat caaactctac cagatactcc gaaataccgg cataatcaat gtcgaagttg
    24841 ctgtaagcac ctctggaatt gacgttgccg tgatcgtaat tgatgtactt gttgcctgct
    24901 tcttcgacca gggagaaacc tgcctgattg ttgggcgagg tccatacatc cgaagcctga
    24961 gctacaaatt cataatcctg gctgtagaaa gccacgtagt cgtctttgtt gataacgatc
    25021 ggcggcactt cattcgcttc ttcaaaggcg gcgatagcct cgttcaaagc tacaacagcg
    25081 gcttgcagtt cggccacgct tgcacctgtt cccttgtcgt acacagcctg ggcggcagca
    25141 atggcagcag caaagtcatc ggcggctatc aaagtggaat cgtattgagc attcgcctcg
    25201 tccaaaacag tcttcaactc aaccgaagaa ttatgatact gataaacagc ctctcctact
    25261 tcgactaagc tccactcgga attggctcct gcggctttat tgccgtagac acccgatccg
    25321 ctggtaactg cgtcggtacc caacaagcct ttgatcgttc tgagcttgta tttgctgccg
    25381 tctatcattt cgataataaa tttgacgtta ttgtcggagg aggctacgtc ttgtgcagac
    25441 atatcccagt tgttggtacc cgtcaagccg acaaattttt catcaagggt cttcaaatga
    25501 taggcattca attctccgga accgatcgga agcaatttaa accactgata ataagcagtg
    25561 tctgcagctg tctttatctt gacgccgtca gctacatcca agtggttcag tgaactatgt
    25621 acaatataga aaaattgagt ggccagcggc tgattcatct gtctgagatt gttcatagct
    25681 gctgtcagga agttttccat ggctacgtac tcgtttaccg aagcacctgt aaaggctttg
    25741 gccgaatcaa tggcggcatt gagtgcgtcc agcttggcag gagcgtattt aaacgactcg
    25801 gctcccaaca tggcgctgtc cactccggca accacagctt ccgcttctcc aatcagggca
    25861 ttcagcgata cttgtttggc tgccagcaaa gtagagacat cgtggctcat caaccttacc
    25921 ctgtctatgg ctacccagtt ggccgtacag cttataccgg taaagccaag cttcagggtg
    25981 ttgtcggcca cagtgacatt gatcatgaat tcttcgggta caccgtttaa gctggaaaca
    26041 ggcgtcgaat cgttgttggc gtagagatag acgttcttta cataagtttt ggcagcttca
    26101 ccggcctggt tttgcttggt tgctatacac caggcactca gggtgtaagt gccgttggga
    26161 agatccgata taatctggct gagattgaaa tcggccaggt tattggcagc agaaacccat
    26221 ttttcgagga accttccttc aaaaccggga tacgcggctg tttgttcacc aaagtcactt
    26281 gcccagccta ctgttttacc ctcggtcata tcgggattca ccacataagc agtaacatcg
    26341 ttggcggctc tgtacgaaac catggcagct accagattgt cgtaagtagt ctggttggct
    26401 gttttagcca cataagtatc gttggcagtc gtaatagcag cctgtaatgc ctcagcctcg
    26461 gccgcggtag cactatagat ataattggct tttgtctgca aagccgggaa tgctgcgtac
    26521 aaagcgatag aactgttggc agcatcaata gccgcgcgca aagcggtcaa cgaagccaca
    26581 acatgcacct ccgaacgaga ggccacagcg gcttcagcat ttgatatggc atcggccaaa
    26641 ccggtagcta cgctggcctg catatcgccg gtgatagctt ccgcttcaac aatcagctct
    26701 tccagagccg taatcatatc gttcaatata aagcccaggc tgtagagttt aacgttgtcc
    26761 agacctaccc agttgttggt gggcagctca gccagcaagc cgatttcgag cttaccatcg
    26821 gtaaccacgc ctaccacctc gtggaaagtc ggctcagtgg tctgaacctg agttttaccg
    26881 ctgttggcaa acaaatagaa gccgtcaccc ccattgttac agaaagcagc agctctcaac
    26941 agataagatc cgttgggcaa gccggtgata atctgagaca tcttgccggt aaagctggtg
    27001 tttttccagt tttcccagaa aggtccggta aagtctccgg tcttgttggt agcaatcttg
    27061 ttgctgctgg ctccggtggt actggtccaa ccattgatgt tggtatcaaa gttaggatta
    27121 acaacggtag tgtcggtgat atccgccttt gcatcggttt tgaggccttc ggcatagaca
    27181 aaaatatcgg taatcgaggt tccgtcgttg gtcgaggacg taccgactat gtccgaggtg
    27241 taatcgggta tccagcggat atacaccttg gaggcataat cggctgcctg aggcaaagca
    27301 acggtcttgg tataccacac cttgttggtc gacatgacaa tggtgtcgag ggtggtaaaa
    27361 ttggtaccat ccaaagaata ttctaccagt tgcttttggt aagcattgta attatacaac
    27421 atggaagcaa ccaccttgat gttgttgtac atggaggcgt tgacacaagt ctggtagtag
    27481 tatttgtcgg ccagaggttt ccagttgacg gcacaatatt tgccttcgta actggctccc
    27541 agcatttcgg acttatccag ccaactggtg ctcgtaccat cggcattgat aagcataagc
    27601 ttagtgacca cattctctgc cgatgcaaaa tcggcgaaac gatctttatt gccggccacg
    27661 tagaaatccc aaccggccaa ataagttttt acatcgtaat tggcttgaag agttacattg
    27721 gcagtcatgt tcaccgtagt atcgggtccg gcacttccgc cttcccagcc tgtaaagtcc
    27781 attatggcgt tggagcgggg gctgaccaag accgaggtac ctgtttcgta catcttttta
    27841 cccgcaaccg gaaagaaagg aggatccaga tcaaacatgt aatctttaac accggccgga
    27901 gcattgattg ccaactcgta cgtactgacg ggcgtaaaat gggcagtaac agtggtatcc
    27961 gacatcaata ccaattccaa cacactggaa tccgtcaata gggtttccga acttttccaa
    28021 ttggaaaatt cgaagccaaa gtttctcaag gctatcagat tcagttccgt gccttcatca
    28081 aaaagcgttc cgggaggatt aatgctgatt tcccctgccc cttcgggact gacaagcgga
    28141 gtgatggtgt atttcggtac gggaatcacc gtgccgtgta ccacaccggt tataaccaaa
    28201 tcaccaatgg cgcaaccttt gccggcacgc atacctttac cccagggata aagcttcagg
    28261 atatacgtgt cgttcgaagc aacaccatcg gtaatatcga gcgatacggt tgtatagtag
    28321 cctccggctt cgttgttacg agcaatgtca atgctcgaca acagagtctc ggcagcctcg
    28381 ccgcgttgat tcataacgtc tattttaacc tggtctcctg tgccattttt aaaggctttg
    28441 aacgagatgt tggtcggttt gaaagtaacg cctttcgagg gtcttaaaat aaaagtgagc
    28501 acataatcgg gtaatctccc gcctatggtc agagccgtag cattgggttc atcaaacaaa
    28561 gtagcagtca gatcgcttga ctgttttttg aaagtattga cccgcaatcc cggacccata
    28621 ataaactggg tggtcgaaaa ggcctctgcc gtatctacat tggccactgt gggcaaaacg
    28681 gtatccgata aaacataggt tgaatttacc gtagtgttat cgaagactgt ttgcgctccg
    28741 agccatgtag gtaagagcat cagcgctaat aacaaaacgc agaatttttt catacatgct
    28801 tagatttaaa atgaataata aaaatattga ttgtaactat tcgtcgtaaa agtacttttg
    28861 aacttcaaca ctgccgtcgc taaaggtagt ccggataata ttgatacccc gggtcagcgt
    28921 tttggtgcgg acacccgtca ggttgaaaat ttcacgctgt tttacaaatt tatcgggcaa
    28981 tttaacttga ttgatgcctg agggtccgtt cacaatattt ccttcctcat agcccggtcc
    29041 ggtaatggca ataataccct cgctgaagaa acgatcgaag ttataggcat agccctcggg
    29101 aagagaatct ttgtgttgac cggtttgcag ctgaccggca ttgacaacca gattgttact
    29161 gatttcccag cctatggtat ctatgcgttc aatgtcggca gtcacgatgg tatcgacccg
    29221 gatcagtgtg tcgcccgagt aaatactgtc gatggttgtg gtaaaagtac cggtaaaagt
    29281 agtgtccagc acgggaactt cgttcatcct cagcgtaggg gcatccaaga ccttataatc
    29341 gccggctacg ggattatcgt aaaagtcatc cataatattc acccataaac gggtaaattc
    29401 gccactgaat gcaccgcctc ctaccaacac cagtttgtcc gactttcctc cggggccaaa
    29461 atcaatatcc acggaattgt tgctgttcag atagagttcc ttgagtatag tcagcgtacc
    29521 gatatcgttt tcaccggtag ctcccggttt aatatgtgct gtcgtaccgg ctttgccttc
    29581 gacggtacaa tcaatgaaac cgactcctcc tacaaacatt tcacgaccac gaatggaaat
    29641 gcgattgata atgttcgaaa aagaggcctt gtctccgttc acgtacaaag ctccggtacg
    29701 cacgtaagca taagcgccca ggtaattcac cgggtaatta aatccctgtc ccacggaata
    29761 catttcaaag ttgcggttat cggctcctcc gtcaatctcc aaatcgtaga gatgaagttg
    29821 attggaacgg aaagtacagg ttgaactgca atgcacataa gcatgcccta tagttcttgg
    29881 gaaacaagta tgcgttgcat cttgcgtgta cacatggata ccgtccggca acaccaaagt
    29941 ccagggaatg gagtgataac acctggcaga agcattatta tacgtaacat tgtctatggg
    30001 gcaagtaccc ggataagttg agccgatcaa aagctgattg ttgccctcct taacttctat
    30061 ggtaagcgta ccggtaaatt cacccaaact tgaaatgccc aagtcggaat ccaggatggt
    30121 agagtcgccg accaccagtg tcagattacc gctgccggtg atggttcctc caaagcggca
    30181 tttactgtcg agcacaatgc ggctgtttcc gttaatcagg aaattaaagg gtacggatgt
    30241 cactttttgt ccctccgaat cggttaaata atcgatgtaa tccatgtttt cttccgcatc
    30301 ggcatcggga aaccaggttt cgggagaatc aaacataatt atttcagcgc cctcttccat
    30361 agtaatcagc gtaccggtgc cgaaaggcaa accgcaggtg ttaaaagcgt ctgcagtaaa
    30421 agttacagtc gcgtcggaaa taataacctg agtttcaccg gcataatccg attcgtcaaa
    30481 aggaaattcc accacataat ctgtttcgat aaaagcctga gaataagctc cgaccgaaat
    30541 caggcagaat agaaaagcaa aagttaagta gtagattttt ttcatagtca attggtttta
    30601 ggtgtgattg ttaatactta gttcgttagt tgtttaatta aggaagaaac catttttctt
    30661 cgcttgaata atcgggaaca gcccgccccg attttaggta tttcccttgc aatacagcct
    30721 gcaaatagtt gttaccacta cccgcagctt cgcggttcat acgccgggcc acgcgtacaa
    30781 ggtcgggaaa acgatgacct tcgtaggcgc attccagagc acattcgtcc ataattattt
    30841 tttccatcac ccggatggag tcttgtttgt cgacacattc ggccaacgtc ttatcggcaa
    30901 aaacagagcc ctctttggat tgcagccagg cgcgtcccct gatgccgaaa ttatacctcc
    30961 agggccccct gtaatagggc gtagtagtat aacgaggatc aaagtaaaag ggttccggnn
    31021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc tccgtcttcg ggtttggcaa
    31141 acaagtcaat cagaggaaaa gagggggcat aagccgcatc gtaactcatg ctccaaatcc
    31201 attcgtaacg aatcctgcgg cccgtcatat cgtccgagaa gatattcacc cactgattgt
    31261 tgtaggcttc catatcgtcc tctttatacc tgaggaaaaa aatctgataa gcccaggtgg
    31321 aataagccgt ggccgtagtc caggtggtag ccgccgccag cttataggta tagcagttta
    31381 ccgtggcctc tccgtcgctg taggtatcca ttatttcttt atataaaaag gcggctttaa
    31441 gataatcgcc gttccaaaga tagagatcgg ccaacataag ctttttgttg atgaaaaaat
    31501 acgacaggtc gtaaccgtcc agattgtatt gcaccaaagg cgaagtgacg tagggatcca
    31561 taaattccag gctttccata tccgcaatca acaagggcag cagttgatcg atagtgactt
    31621 tagccgaatc ggaatatgcc cgcatatctg ccagattgac aatgggtttt gtaatataat
    31681 acacccgacc gaactgtgct gccaactgca aatacatata gcaacgcatc gccgtcaggt
    31741 cggcgtaacg ctcacggaat tcttccggag tcatccggtt gttttcgagc attattttaa
    31801 aattttccag agcatcgttg caattcaaaa tcacattgta atacgaagtg ggatcggcca
    31861 gggtattcga ctcggaagga ttggcggcat caatttcctg cagttccgtg cccgaatagg
    31921 ccgtcaaatc agtgagctct ccgcgaagct cgttcaggat taacatctga tcggccatac
    31981 gcataaaaga cgaatacact cccaaaatgg cattatcggc atcttcggtt gtcttgtaat
    32041 gtttgtcgta ttccagcgca tcgtcgatgt ccgtctgcac gcaggaggac aacatcaggc
    32101 tgcctatcaa aacaactaag gctatctgtt tgtttgtctt cataatggta gaattcttat
    32161 gcttatagtc caattttaat acctaaataa aaagtccggc aagtgggcat agtgaaagca
    32221 tccacaccgt aatagagcgg catttcggcc gaagcgctga tgatttcggg atcatagcct
    32281 ttataagctg tcaggcaaag caggttttcg gcggttccgt atatggtgag atttcttaaa
    32341 acaccgcttt gaatcggaat agcataggcc aggctcaggg ttttgagctt caggtaagaa
    32401 ccgtcctcta tccaacgatc cgaaaaagcg gaattcccca tgggatctcc atacactgcc
    32461 ctgggcatag tggctgtttg agcctccgat ttccagcggt tgagtacagc ctgagtctga
    32521 ttggcaaagg aagacatcga ctccagattt ctgcgggtat aattatagac atcgttaccg
    32581 agggaatagc ggaagacggc cgagatactc aggcctttcc aggcaagatc ggtccagaga
    32641 gagccgtgca aatcgggatt gggattgcct atctgttgca tgtcgttctg atcgatcaaa
    32701 tcatcaccat tgacattgac ataacgcaca tcgccggctc cataaggcac ttttaccgtc
    32761 cccgacattt tatagagttg gttttcggca gcttcggcgg ctgtttggta aatgccctcc
    32821 gttttgtaac cgtagaatat acccaatggc ttaccaacct ctgtcctgac caggcctccg
    32881 gctatagggg tgttgaaatc accggtgctt agcgacaaaa tctcgttttg gtaatgagag
    32941 gctgccaggc ccagatccca gctaaatgct cccttttcga ccagtcgggc gtttagcgat
    33001 gcatcaatac cacgatttcg catttcaccc ccgttctcta tataaaaacg aaaaccggta
    33061 taagccggag cttcggcatg tatgagcata tctttgacag tgctgtgata caaatccaga
    33121 cttatattca atctttcgtt cagcagggcc agatccaggc cggcactcag cttattattc
    33181 ctctcgggca tgagtttctc gtttaccaga ttccccctta ccaaaccgta attgcccagg
    33241 aaattttgag tcacgtaata gcgcttagcg gcatagttcc ctattccatc gttgccggtc
    33301 atactgtaat tggctctaag tttgaacatg tctatccatt ggctgttctt cataaactct
    33361 tccgaagaaa tcaaccaggc tgctccaagg gaggggaaag cctgaaaagc ggcaatttcc
    33421 gatccgtaac gcgaagaagc atcgtaggaa agcaaggcgt tgacaaaata tctattcttg
    33481 tggttgtaat cgacgttgcc atacagggcc agccaattcc agtttcctat agctcctccg
    33541 gtctggtaca gtctgttaga gccggaacct atggatacaa aataattgct cgaagagttg
    33601 taacctttgc cgtaatcgtc ttcggcacgg gaattcagat aacgcaagcc cagagaggca
    33661 tccagccgcc g
//
LOCUS       DAOSTD010000007        33430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1452_length_33430_cov_14.081139, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000007 DAOSTD010000000
VERSION     DAOSTD010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33430
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1452_length_33430_cov_14.081139"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..788
                     /locus_tag="PLI37_00900"
     CDS             21..788
                     /locus_tag="PLI37_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOQ57608.1"
                     /translation="MKIISYNLNGIRSALNKDLTAFLQEEAADVVCFQETKAQPDQID
                     SSLFWEIGYPHCYYHSAVKKGYSGVAILSKREPDRIVVGMDNHKYDSEGRILRADYGD
                     FSILCVYVPSGSMGDVRQQFKMEFLSDFIDFVGELRKERPNLLICGDFNICHKPIDIN
                     HPERHQNASGFLPEERAWMDDWAQTGLIDTFRQFDPSPEKYTWWSFRANSRARNLGWR
                     IDYHWITTTLESNLKGAAILPEAMHSDHCPVVVELDL"
     gene            819..1997
                     /locus_tag="PLI37_00905"
     CDS             819..1997
                     /locus_tag="PLI37_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOQ57609.1"
                     /translation="MAVNRDTSSFFRLVFFLVALIIIAASTWFSNDLGKELAAEEQKK
                     IELWAEAIRLLAQEPQPGEEDVRFDYTLTLKVIEGNTNIPVILTDGDNNVQSYANLNL
                     SKKHPEADLEKKAAHLIQKNRHIQVKLDENTYQYVYYDDSDLLKKLRYFPFVQLGVMF
                     VFLAVTLVSLTVFKRSEQNRVWVGLSKETAHQLGTPISSLLAWAELLKLKDIDPALLA
                     EMEKDTQRLRTIAERFSKIGSRPDAEYHALAPVVENAVNYLRGRSSQKVIFRTHFPKE
                     ELPVLLNIPLFEWVVENLCKNSIDAMNGEGQIDIVAAEVKKQVLIDICDSGKGIPKKH
                     FKAIFRPGFTTKARGWGLGLSLVKRIIEEYHKGKIFVKSSDPGKGTCIRIVLRKALKN
                     "
     gene            complement(1987..>3362)
                     /locus_tag="PLI37_00910"
     CDS             complement(1987..>3362)
                     /locus_tag="PLI37_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57610.1"
                     /translation="QCYANHQFSGPYLFFNPGGKRIKSFRTDDAGLNFRWGKKAAFIN
                     LYSYGISESFRAEDYIFGKEISNNAQKIRQFNVFNLGWHKDCSYLELNLGNNLSRSDF
                     GLGILQSVQKEREWKTSLNLKFLPLDFLLLQTGLAAEYLSLDFKTDKPALAFSMSDSV
                     PMLHQDTSIACFIPEAVLYAKLQLPGQITLGLGLRNKILSDKLPAYLSYQVNLRKKLE
                     EKHSFLLSGGNYQACFYPVYRYTRFSLQTSRQLSLEYTYEGEQLFIQAAIYGKQEESP
                     TYYGPEYKMKILQRDIRGLECSAEYEWNRLRASLSYTLLDAHIKSDSTWHKAENNLPC
                     FLKAYLSYTGKHLLSLSCSGIYRTGLRYTPVESGRYVVEAYAFEPQHGAYNSRRYDDY
                     FRLDLSLNQVIFGKNQRRLVVFATVGNVLNRKNQKARLYSPDYSDTIGYSHYQGRTYY
                     LGAQVSF"
     assembly_gap    3363..3462
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3463..3959)
                     /locus_tag="PLI37_00915"
     CDS             complement(<3463..3959)
                     /locus_tag="PLI37_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57611.1"
                     /translation="MVKHGFILILVCFFPLCIQARSLLEGRVCDQQGNGLFSVLVCLK
                     QQENSCCYSDTKGYFSLDIPDTATDDSLFLFFVGYKSLNIPLKELDLSSFTELRLQED
                     NRMLAAAMIEHNPRLSEEFSLQKINKLDIYLSPISDGDPLKALSFLPAASNTSENANP
                     EFRGSP"
     gene            complement(3935..5101)
                     /locus_tag="PLI37_00920"
     CDS             complement(3935..5101)
                     /locus_tag="PLI37_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57612.1"
                     /translation="METATWQWNEDFLILDNNNSELILKNDSNGALVSLSEAEYIFLH
                     YYSIDNDYEKVCSLMQDYHIEKDLFDALTNKARQLGLLIRQGSIRKTPERSTGLKITA
                     YFLFVRFLRITGKLCGLNLIAECKGNLRFYKLCSLDLHNTVLHKMALSERLQKILLPL
                     YILTMVLLISLLWLLPGAEIRFSGFGMTHIPVFGFFLLLVVSIFACLFIHETGHYLCY
                     MRYGGKSSKMGLGLMFLVFPVMYTHIDQVQLWHDKRRKIKLSLAGVLMDVMIMLLLLI
                     ILSHYHKPDLFALLTACLFYYYAVQLLSNLNPFFPGTDGYYVFEDLIGGERLFGNSYE
                     CFKGFVKHPFAEKISLKRCGYLLYFLIACINISLYWFMVTALMTFPLWSNMVLS"
     gene            complement(5109..5924)
                     /locus_tag="PLI37_00925"
     CDS             complement(5109..5924)
                     /locus_tag="PLI37_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HOQ57613.1"
                     /translation="MDIANLFYDQGLSQYALDFMQSSSMTPFNQLENAQRIQSIMNIP
                     YYSRQIIAHTLERGTTERIKLNTLFNEESIALRNIQIKRKSTRSFHEHINLEELANLL
                     LNSYFITERFEKHSHHLSRRSIPSGGALYPVDLYYINLNTIGLQRGVFVYNPHEEALE
                     LLQPAGDKKNFFRQLRKCFPEAVLGSWNLDLISGIVVFGGVLNRSSCKYGDRGLRFVL
                     MDVGALCQNLHLSAAAGGVSCCAIGGYIDQNLNDFLGFQQPDECALLSMFIGK"
     gene            complement(5930..6646)
                     /locus_tag="PLI37_00930"
     CDS             complement(5930..6646)
                     /locus_tag="PLI37_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57614.1"
                     /translation="MKQLFLLLLLAAVLPLQAQNLQTEIDSAFIHYFKTGRMEEIDRI
                     INALHLENKSSNNRYLNYWTAYGRYKKSLCAQSNPAEEFQKTARIELSEALKCLNKIK
                     NKESEDYALLAILKNYSVAFVANVKIPFVSGEAKKYAQKAIDMDPQNLRAYLALGIRD
                     YYTPAVYGGKTQFEELFLKAISLRDKYGNNPNDPSWGKNDAYYYLLDHYCRKDMEKAR
                     HLFAEAIELYPDDPRILKFK"
     gene            complement(6657..7940)
                     /locus_tag="PLI37_00935"
     CDS             complement(6657..7940)
                     /locus_tag="PLI37_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HOQ57615.1"
                     /translation="MRTICHTAAGLLSEAMIRIPNRRFIPDGLVQSVVSGKNLDWINC
                     RRREIQGSSIAFDEEQSHRGALGEFLERYACATYDSEDFKVSSFSELSKSEPALAPEF
                     FRYYSDEQYERLRELNVYPLGENDLIEWTVCNDFITGKSYWMPAFSIYMPYFSKVNSP
                     HNYMVGTTSTGTAAGKTSRDALISGFLECAERHAFALFWYHQDALPYRSYTKEVILKH
                     YHKNKTVCRLFQNSAVQIKCFDLAAFSPVECMVVFIYFRYKNKIYQSLGCAARFSKTQ
                     ALIKACLEAYQGVEYAISLNEKKLLPEEPDLSRIDDFDKHFHFYNQYPQFRKEAPILR
                     EAARFDSGDEKIYRADPDNKCMDFTVEELKKVGLKHLMYKDISPIDAEELQYKIMRVV
                     TPGWSLLTGNHNWPFLGYCLNGNQTLFTAYPHPFP"
     gene            complement(7943..8797)
                     /locus_tag="PLI37_00940"
     CDS             complement(7943..8797)
                     /locus_tag="PLI37_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57616.1"
                     /translation="MKIKIQNMDIWECRDAIYCKRSDGNLIKIQSKANHKIIEVLKHL
                     RSVASLEDFQSEFVSRTEFSEIVKFLLSNNLIYKECEQKKQLKKIGFYGNKNVFKHLG
                     SDLKTEQFELSFNPVYENNSVQGLHLILTISPVFDHYSLLQDLSYRSYKSMIPVLYVE
                     FSPSTLTLGPLVVPAMNTPSLGCYMKRKAVNLSSLALYSEFILSEEKQRFHNVDIRSY
                     AHYHAGMQLIAIELERFWSFKGKLSTHLMGKSYTIDFLQYKTEETQILKDPASSLFKR
                     PAFSPFNA"
     gene            complement(8901..9104)
                     /locus_tag="PLI37_00945"
     CDS             complement(8901..9104)
                     /locus_tag="PLI37_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57617.1"
                     /translation="MKTLEMTMKVVEDRSAPLMLTERNLPGMCCCTCCCSCCFGTGEG
                     TGGSSTVTTPGDLGNSLSTSRNI"
     gene            complement(9336..10004)
                     /locus_tag="PLI37_00950"
     CDS             complement(9336..10004)
                     /locus_tag="PLI37_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOQ57618.1"
                     /translation="MEQLLDKIAYCVEYGKINLASPYPPDMRGQEGADEYTKRALEQG
                     VSPSDVLTKGLIVGMGKIGVKFRENKVFVPQVLMSARAMSTAMNHLKPYFNSGAVKRK
                     GKFIIGTVKGDLHDIGKNLVSMMVEGSGFEVIDLGVDVPAEKFIEAIQAHPGAYVGMS
                     ALLTTTMASMEEINKAIKQASPQTTTFVGGAPVNDEFRKQIGADYYSPDPQGLTEILT
                     KLTA"
     gene            complement(10066..11145)
                     /locus_tag="PLI37_00955"
     CDS             complement(10066..11145)
                     /locus_tag="PLI37_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57619.1"
                     /translation="MTYIKNPAFSNEPLPVEIVFHPSWWHKHCGIDFDEDFFYHPLKR
                     VESERRMEKELYERFGQFGLGSDYDKDLPLIGAVHNAAGYLLSEMLGCEIRYFADSAP
                     QVIPAQKTDFHVDVDAAFAGPAFKRLEKLMSELKSRYGYLKGDVNWGGILNLAMDLRG
                     EEILMDFLLQPDEVKLYFKKIAELIERFFCFIQQETGSNSISVNRNVINITPAVYLHS
                     ECSHTMISEEQYEEFLLPFDVEWSKKYRPYGVHYCGPDPHRHAAQMAKIPHLDFLDAG
                     WGGDMVCLRKHLPSTFINIRLDPVSINTMSCETLEQHIRRLVNDCANPWLTGICCINM
                     DDKVTDDKIHTIFSTAQQLRREYRL"
     gene            complement(11230..>14019)
                     /locus_tag="PLI37_00960"
     CDS             complement(11230..>14019)
                     /locus_tag="PLI37_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ57620.1"
                     /translation="RNSGSWTKIKVPSCWEQEGFGIYQYGMPFYGKANPPGIANEKGL
                     YKTRFKLPAEWEGRIIRLVFEGVMTDCSAEINKRRAVKLHQGAFYRFYADVSDRVFFG
                     DKENLLEVTVSKESENSSVNLAERRADYWNFGGIFRPVFIQALPAQSIDRTAIDAKAD
                     GSFYAEVFLSTALDGDYSIEAQLLDREGKNIGKALKAELRPGSDKVIVKGQFTDIKTW
                     TAETPHLYDVQFSLLKSKEAVHIVKEQRIGFRTIEVKESDGLYINGQKVMIKGVNRHS
                     FRPETGRTLSREDNYNDVRLMREMNLNAVRLSHYPPDPAFLDACDELGLYVMNELGGW
                     HGKYDTEVGKQLVKEMVTRDLNHPSIIWWSNGNEGGWNTELDSEFHPWDPQKRPVLHP
                     QGNFGGFETMHYRSYGESQEYMRKPEIFMPTEILHGLYDGGHGAGLWDYWVMMRRHPR
                     CAGAFLWVFADEGLMRTDQNGRIDNVGNYAADGIVGPHHEKEGSFFTVKEVWCPVQIK
                     ALGRTVTCTERTEALRQDQNSFIADPEAFVGKFHVENRYDFISLEDCRIKWSLANFAK
                     PGDKSGGHNIIGQGEQAGPKLAPHQSGEFQLKLPANWKEADVLYVSVLNPRGEEIYTS
                     GWYWKSDRDYTRAALAGQANTSPAKLSQSREGSTLTIESGATRLQFDTETGYLIQISK
                     GNKNLSLSGGPRFIAARRSDRSMDVFYNHDDKAAKSKDRIYADISGNSRLTDLEVEEQ
                     EDKILFKTQYFGNLNSVCWTITDREEITVDYEYQYEGVVELAGICFDYPEDNMQGMRL
                     LGKGPYRSWQNRMHGTLFDVWDKEYNDPIPGESFIYPEFKGYYNDWKWVDFQTAQAQL
                     WLGNGTPGSFLGVYTPRDGRDALLYTIPQSGIAVLDVIPGVRNKVNSTDLVGPSSQAK
                     WLSGPQKGSLQIRIR"
     assembly_gap    14020..14119
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14120..14360)
                     /locus_tag="PLI37_00965"
     CDS             complement(<14120..14360)
                     /locus_tag="PLI37_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57621.1"
                     /translation="MKAKNLITFSLIMLLCGSVAEISAFKKQSSTPGFDPLQTAEIYL
                     SGHGPDDAVEWDFMCTGGRNSGSWTKIKVPSCWEQE"
     gene            complement(14411..17125)
                     /locus_tag="PLI37_00970"
     CDS             complement(14411..17125)
                     /locus_tag="PLI37_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57622.1"
                     /translation="MKTPYYLLSFALLLVFSACQPKVQLLTTGSDSPRGNYAKERLSN
                     SLLSNGYTIVDENPAGKATKIYLGCQNDLNFQQTLQEQGLAIEPVEGKEAFRIVADGD
                     KIAVCGADESGLLYGCMELIDRLKTNGRLPKKIDYSDAPDMVLRGTCIGMQKMYLLPG
                     RKVYEYPYTQELFPWFYDKELWIKYLDMLVENRMNSVYLWNGHPFASLVKLPDYPYAV
                     EVSEEDFERNEEMFGFLAQEANKRGIWVIQMFYNIIVSQPFADYHKIPTQDRKVEITP
                     LLSDYTRKSVAAFIEKYPNVGLLVCLGEAMNTHEDDVEWFTETIIPGVKDGLKALGLE
                     QEPPIVLRAHDTNAEMVMDAALPIYKNLYTMNKYNGESLCTYQPRGPWTATHQALSAL
                     GSVHISNVHILANLEPFRYGSPDFIQKSIQAMHNIHGANGLHLYPQASYWDWPYAADK
                     IEDGSRLLEMDRDWIWYQSWARYAWSADRDRKEEIDYWSNLLSELYDCGKQGKYILEA
                     YEQSGEISPKLLRKFGITEGNRQTFLLGMFMSQLVNPNRWTVYPDFANSCGPEGEKLE
                     VWAYREWHGEAHVGETPPQLIAETREHARKAVEAIDKVTRVRKNKEEFARLKNDIYAY
                     KAFTDCFTDKLEASMLVLRYSYSNEISDLEQAYSLMESSLDHYRKLEELTRDSYLYAN
                     SMQTGMRRIPVTGNNGANKTWTELLPQFEQELARFDANIQKLKSGNQTPENLKVLSPA
                     EVKLISPARAEFYQLQEGASVYSDRPFKIKALAPELSQLKALRMSNRQQLNENIRLRF
                     NSREAVDLWVAYFKPFRQGDYMPAPRLETDASANQYGQADIKIANAMEIENLPAVNVH
                     QYRFEAGEHNLNLGKGLCLVLGFSKPEAVFSTRDAGFMEKTAIDWLFY"
     gene            complement(17350..18111)
                     /locus_tag="PLI37_00975"
     CDS             complement(17350..18111)
                     /locus_tag="PLI37_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOQ57623.1"
                     /translation="MKKNLILTFLVVFSCLHLHGQNTESISITLDSDQYKQLEQHFAE
                     KEKAKMRSTDWARFYRYEAMNDSIQKPAKVVFLGNSITEGWYRRHPEFFIENGFTGRG
                     ISGQTTSHMLVRFQADVIDLKPKMVVIMAGTNDIARNNGIISHEHIVQNIKSMCELAQ
                     FHKIKPVLCSILPAHEFGWNKELTPAADIKLLNEMIKAYADANKIPFVDYYSALVDER
                     GGLPVEIAADGVHPNLQGYAIMEPIVLKTIRKYVK"
     gene            complement(18138..21221)
                     /locus_tag="PLI37_00980"
     CDS             complement(18138..21221)
                     /locus_tag="PLI37_00980"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOQ57624.1"
                     /translation="MKTGKFVLFLCFLQIQAVILAQNNWYVKATGTSSENSGVNWESA
                     ISLDDALARSSEGDSIHIAAGTYIPQILLTNGRVEKEKCFEIKNNIALIGGYPADASG
                     HVKPEPAIHPTILSGQLGSELNAFHVVVITAASRRNRPVKLSGLIIRDGVADNTRRTT
                     IDNIVYQQGVGGGLIAEKGNIDISDCVFTNNKAFDGAGCWIQNATLNVRNSKIIGNST
                     SRGKAGGIYLNRVSFNMEQVIVSDNFAETDGGGIYLVGSNGYVNQGLISGNTVNNSAG
                     GVLAAASSTVYLHNVTIGHNEAKTNGGILLENTFMSMANSTIAENTAFESGGAGGVSD
                     NSRIEATSSTITRNSAHNSAGGFIMGGNGILNCYNSILSGNGVEIEVVAGTTSTSRFY
                     QSIFDTQYYNVDGEPDGQISFDADIMLDSLKDNGGFTPTCKILHPETNPVNTKGLTLS
                     EMTLLASKYGLPGFVILKDQRGKDRLTSNIAIGAYQDSVFTRPIHPNFWHFIIYGQSL
                     AEGYQSYPPITSEPVPNNYMIGSNVCINNGNVNCSQIAPLFSSPTPDDVIRNFARSWT
                     DGAKCENSVLGAANHIQHKLTRPVSILATSCATGGKTIEELSKHCQRSPYYYHRDFIR
                     SINSAASFVKRNNYNIECPAIFWMQGETNYSRNQPGLISLNGSTTDKSGYRDLMQILK
                     NDMQSDIMSLYGQQEKPLFITYQVGGKYIKGFEQNIAMAQLEASNQEADIVCAGPVYL
                     MTDRSAGHLDPNGYRWFGEMLAKVFYKTYIEKETFMPLQPSKITKENNHTLRIDYLVP
                     CKPLVLDTLLVAFEKDFGFAIKNNGSLVNINSVTLHENGESVLISCFDELNGDIEIAY
                     AGQDVLGHGNLRDSDPYQSIYTYVDLDGKDENGEYIFEHDAGNTSLHPRYEPRDENGV
                     IYGKPYPLFNFSAHFYYKLPAGTTELIPPSLSDNTSIPAVKENLSTVRLIYHRYNQIQ
                     ISGNWQYAHFYDVSGKLCQKTNHTSIIDTGSLIPGIYIIRVFENDKIHNLKYLRL"
     gene            complement(21282..22112)
                     /locus_tag="PLI37_00985"
     CDS             complement(21282..22112)
                     /locus_tag="PLI37_00985"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOQ57625.1"
                     /translation="MKRLFIAIMAIPFLFGCAKTENRPTLRIMSFNIRYDEPKDGENN
                     WNFRRQACVDMINDQQPDVFGIQEGLIHQVNFLDSALADFAFVGEGRDSSAKNNEYTA
                     LFYNTGKFDLLSSGTNWLSETPEVSSIGWDARFNRIVTWAHLKNKADNKEFVVLNTHF
                     DHLGVKARIESAKLIVQKAAELSGDTLMTFVVGDFNTLPDDPLLDPIYQYLKSSQSTA
                     AKTDSLGTINGFRVTQKDRIIDYIFYRSATPIQYQTIVKDYGVPYISDHYPIMTIFEY
                     "
     gene            complement(22178..23029)
                     /locus_tag="PLI37_00990"
     CDS             complement(22178..23029)
                     /locus_tag="PLI37_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOQ57626.1"
                     /translation="MKTPIKNLTLLFVCISTFGLMLFGCKPADYKFPKDNIRLAVYNI
                     RGEDPNDGVNLWMYRKDSLIKVILENNMEIVGLQEAVPFQLEDIVAGTSYESVGKEGL
                     LNPVIYNGKRFGVIEWNMFWLNENNEPEVIGWDGKYARYCTWVKFKDKKTGKEFYFFN
                     THFDHRGLMAKTNSATLISKEAKRIAGNTPFIIGGDLNSKSNTEAYRILSENFVDSRK
                     SAPQERVYGPVGTAHNFGGVHPVQIDYIFVTPEIRIHTYRAIDEAYENNLFPSDHYPV
                     YVDLTIK"
     gene            complement(23042..25516)
                     /locus_tag="PLI37_00995"
     CDS             complement(23042..25516)
                     /locus_tag="PLI37_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase C-terminal
                     domain-containing protein"
                     /protein_id="HOQ57627.1"
                     /translation="MRKVFYFLLIGLFVFTANASATQADSITGFAKTDSGDPIPNAVI
                     SDGYAVVKTGPDGYYSFPRHDRTEFVFISVPEAYEIPMGDDGVPQIYARVSTSGNFSH
                     NFTLVPFTDGGAADFNHVMIAIADPQVRRDYETWRFRNETIKDIKELKASYPAGTPFY
                     GVVVGDLVWDDYGRLEEHKASVAGTGFPVFNVIGNHDHDMTKCGDIESDHYFKAAFGP
                     TYYSFNRGNIHYVMMDDIDYIGCNSKEYTHNITQNQIDWLIKDLAEVPIEKTIVISVH
                     APIQGSNVANRQTVYNLMGGRRNIQRIIAGHHHRLTNNEVSARLFEHVVGSANGAAWS
                     GDYCSDGTPNGYGVFEAGDRGFNNWYYKATGKSKDYQFEAYPTGSFDESNGKGDCIIA
                     NVWNYDSRWSVNIYENGVKKVMQQFTGVDPRMYDFLGKVGDTRPNYPGSDGGTTVSQN
                     PGPSATSHIFSYKPTDPDAEFIVEVTDRAGNVYTKKLLRNIMVASFDKEEDDYIYRQD
                     FNTLPFYPNHLLTDRTAKGTFVPGHSPKGWYVTTSGNVRPSGSIIPDWSTFNYYRINN
                     GDQDAGWIYSYGEGNPVVPNEHSAERALGSLISSYHRNIHFGVVIENNTGEFLSGLEI
                     DYDGEMWRAGTNPANQQKLSFSYAVNPDAKAIRERRLWISEVATTTVDSLSFVSPVNN
                     TAAQSGLTDVAIDGNNAANRVRIKGKIDAPIAPGQVVLLRWEDIDDRGNDHGLAIDNL
                     VVKAILDETNSTGKQIEDRSTFYNIEKKIFFTGNRHENYVTIYDLTGEVIFQKTTQAN
                     ILDLTGIIDSGVFIVRVGNTAKKLVL"
     gene            complement(25725..27905)
                     /locus_tag="PLI37_01000"
     CDS             complement(25725..27905)
                     /locus_tag="PLI37_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ57628.1"
                     /translation="MKKHLSLLIVFLALVCNVNAQVMEWYTYWGSNVAGNQIQPVRMV
                     VDNAGDIYIAALFGGNSVQILDQTFPSQSGTDLGDATILKMTPDKEIVWSRKLVNSGE
                     AAISDMIIDNEGNLVVAGTFKGMLQADENDSMAMEDPSGYAALSIFVIRYDSNGNVLK
                     MWQLPAEEATVESVTADGSGNIIISGTFGSEMSFDPANLNGFVGNTQYWNQMYVAKYS
                     KDGALIWVKYQQTAEASFVNAFSKADADGNIFVSGAFTGSTTLAGTNLSTQTAINDLF
                     LLKYTPLGEESWVRHIKGSRSDAVTGIEISPIGEVAVMSTYYSEDITIDQSTDTLQNG
                     FATKEGEAKAHHIGIFTFDKDNGDYRWWYSFGQGSTVGGGGAVGSSIRVTDEGVWYVV
                     GTMSSRFGDLYSHSNFGGNARSGLRTVDGIWMQHNTNGGGDGLYFVLTRDGKLASVAR
                     PGGTQTEVIKDIALSPDKNHVYMLLEIRARANVIYTCPDNMFDSFTDLHNNFPERKEM
                     YTLLQVYCPEDPVENKYTNTYVGFTSTLLGKYKLPEIVPNQLPEFTVGEEYNQPLSIA
                     SPAGKQQFYQLLIPEELNFYNNALIGTISTAENLCFGVIATDSTANPGPITYYAQDPN
                     GESIRGLSRNVRYMPLNAKEGSSVKNPLVGNVTFYPTVTSNVLNIQTSETNYVVHLYN
                     QVGQLIGKYSNPISIDVNNLPAGLYHAQLKSMTGAVETAKFIVK"
     gene            complement(28026..29513)
                     /locus_tag="PLI37_01005"
     CDS             complement(28026..29513)
                     /locus_tag="PLI37_01005"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ57629.1"
                     /translation="MKKYFKYLLIVLIPGLLNSCDTEFLNLRNPNAFDVYKFYQSEQD
                     MEDALLAAYQATRSFYDWMFFATEMKSDNATTFDSGTSGGLYYTFVSHQVTSNNTIVA
                     RIWNGLYNTIYKANLVLKHIDGVKMSDESRERIIAEAKFLRALSHFYLVRLFGPVPVV
                     DKVVETITEANAMTRQPLSEAYDLIISDLETLANSTVVPAFYDAGNDNFGRATSTAGA
                     ALLGRVYLHMAATQNKDEYYTNAITYLQKAITLKGYDSFPAGATYPRVFGTSNEGNEE
                     IIFQCMYLSTSSESSPFAQYFQPMGLKGLTSQINGRGFNAGQENLFLEYEAGDRRTPI
                     AIMKAPNNQYYTKKYVDLSNSTGYHGNNWIEIRFADVFLMLAEAYEKKGMTNEAKNYL
                     NKVRVRASLPKYDDSNAAYHAKYPTLRDAIFHERRVELSFENHRWFDLRRLYPNDNDF
                     VAFMQSVEEPNVGNKYTSFKAYEILLPLPYDEVFLNKNLHQNDGY"
     gene            complement(29530..32994)
                     /locus_tag="PLI37_01010"
     CDS             complement(29530..32994)
                     /locus_tag="PLI37_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ57630.1"
                     /translation="MKRKMKFILMILVLCCFSFSAFSQSITLNVRNITVKEAIEQLKQ
                     NSGYTFVFEAGDLDTGKLISVQANQKSIEEVIEQILKDQNVSYEIRDKNIVVTRGNTA
                     RQITTKNITGLVTDVNHEPVIGATVVDLSTNKGTVTGVDGKFTLEVTNQSTLQVSYIG
                     YTTSEVVVGNQQNLTIQLNEDAIMLDEMVVIGYGTVRKSDLTGSVASIKTEELNRSTA
                     NLEQSLVGHTPGVQIKQVSGAPGSGTSIRIRGINSVYTSVEPLFVVDGFPASKDVYIN
                     PSDVASIEVLKDAASAAIYGSRAAGGVVLITTKRGEKDKTRVELDYQFSIQELSRKID
                     MMNADELRELHRDGYNNTYFDFLRINNIYGSDEERWTHSREDDNDTRTANGASKLMLL
                     SPDFLATKYDTDWQDALFSKAPMHRTGVSISGGKSGYKYMFSLGYLDQDGIVAPSNHK
                     RITSRLNMDVDVSDRLSVGVNSNMFYVTERTVRTDGLAFNDGLILNTLGMPPQYPVYE
                     EDGSYATGWTYRNFPYSTFAGENPVALKDLIQQYYTRTRYSVNTDARYKFFDGLYAKV
                     NAGLQISDQIYRYYRPAGEIGQSNGFPGSFDLHARASNDRDFNTDWLLETTLNYNKKF
                     GKHTVNGLAGYSMQKKNYDNIDARGQGFKNDRIPDLSAAGPTATDENGTSAVTSRAAW
                     SIMSFFGRVIYSYDSRYVINASLRGDGSSRFGPAKRWGYFPSVSAGWKMSNEEFFASL
                     VDIMTVRLRASWGISGNFNISNYRHIPNMAQGTYNLGQGTVFTYYPSAFVDQKIGWEK
                     TNQTNIGADLGFLKGRLNLIANYYHSVTNDLLYNTSVSAVTGYTGSWTNLAEGKVYNR
                     GFDLQIDGSLIATQNVKWNAGFNISVNRNKVMGLSDEIITKAQRGQITHITRNGLPIG
                     SFYGMVSQGVINAEEYANILIDKANQSTPGYELVGPPVADYDNVYIGDVKWKDVDGNG
                     KITEDDREIIGNNYPDFTFGFNTSFNWKGLSFSATFDGQYGADVINFSRYYIANQEGG
                     VNTMRSGIDRYRDEANPGNGLIYRANRSAKNLNTKFSTYFVEDASFLRCTNITLGYNI
                     SKNRFLNKLDISNVYVFGSVDNALMFTDYLGYNPDVDYNSGNLAPGIDFGTYPIARSY
                     SVGVKLSF"
BASE COUNT         8682 a         7373 c         7502 g         9673 t
ORIGIN      
        1 gacaaattta actgatcagc atgaaaatta tctcttacaa cctgaacggt attcgatcgg
       61 ccctgaacaa agatttaacg gcctttttac aagaagaagc agccgacgtg gtctgttttc
      121 aggaaaccaa agctcagccc gatcaaatcg attcttccct gttctgggaa atcggttatc
      181 cccattgtta ttaccattct gcagtaaaga aaggctacag cggcgtggcc attttatcca
      241 aacgggaacc cgaccgcatt gtggtgggca tggacaacca caaatacgac agcgaaggcc
      301 ggatattgag agccgattac ggcgatttca gtattctttg tgtctatgtg ccctcgggca
      361 gtatgggcga tgtgagacaa caattcaaaa tggaattttt gtccgatttc atcgattttg
      421 taggagagct gcgtaaagaa cgtcctaatt tgctgatttg cggagacttt aatatttgcc
      481 acaaaccgat agacatcaat caccccgaaa gacatcaaaa cgcttccggc ttcctgcccg
      541 aagaaagagc ctggatggac gattgggctc aaaccggcct gatcgatacc ttcaggcagt
      601 tcgatccctc gcccgaaaaa tacacctggt ggagcttcag agccaattcg agggccagaa
      661 atttaggctg gagaatcgat taccactgga taacaacaac gctggaatcc aatctgaaag
      721 gagccgccat actacccgag gccatgcatt ccgaccactg cccggtggtg gtggaactgg
      781 atctttaacc aaactgtttt taaaaaatcc gtaaaaccat ggctgtcaac cgagatactt
      841 cctctttttt ccgtcttgtt ttttttctgg tcgccctgat cattatcgcc gcatctacct
      901 ggttttccaa cgacctgggt aaagagctgg ccgccgaaga acaaaaaaag atcgagctgt
      961 gggccgaggc tatacggctt ctggcccagg aaccccagcc cggagaagaa gatgttcgtt
     1021 tcgattacac ccttactctt aaagtgatcg aaggcaacac caatatcccg gttattctta
     1081 ccgacggcga caacaatgtt caaagctatg ccaatttgaa tttgtcgaaa aaacatcccg
     1141 aggccgacct ggagaaaaaa gcagcccatt tgatacaaaa aaacaggcat atccaggtta
     1201 aattagacga aaacacctat caatacgtct attacgacga ctccgattta ttgaaaaaac
     1261 tccgttattt tccttttgta caattgggag tgatgttcgt ttttttggcc gtcacactgg
     1321 tttcgctcac agtgttcaag cgatcggagc aaaaccgtgt atgggtgggt ctgtccaaag
     1381 aaacggccca ccaactggga acaccgattt cgtcgctttt ggcctgggcg gaattgctca
     1441 agctcaaaga catagatccc gctctgttgg cagaaatgga aaaagacacc cagcgcctgc
     1501 gaaccattgc cgaacgtttt tccaaaatag gatccaggcc cgatgccgaa taccatgcac
     1561 tggccccggt ggtggaaaat gcagtgaatt acctcagggg acgaagttcg caaaaggtga
     1621 ttttccggac gcatttcccc aaagaagaat tgccggtatt gctgaatata cctttgtttg
     1681 aatgggtggt cgaaaatttg tgtaaaaact ccatcgacgc aatgaatggc gaggggcaaa
     1741 tcgacatcgt agcggccgaa gtaaaaaaac aagtcctaat cgatatttgc gacagcggca
     1801 aaggcatacc caaaaaacac ttcaaagcca ttttccgtcc gggtttcacc accaaggcac
     1861 gcggctgggg gctgggtttg tcgctggtga aacgcatcat cgaagaatat cacaaaggca
     1921 aaatcttcgt caaaagctcc gacccgggca agggcacctg catccgcatc gtattgcgca
     1981 aagcgctcaa aaactgactt gggctcccaa ataatacgtt cggccctgat agtgtgagta
     2041 gcctatggta tccgaataat cgggcgaata gagacgggct ttctgattct tccggtttaa
     2101 aacgtttccc acggtagcaa agactaccaa cctgcgttga ttctttccga atattacctg
     2161 attcaggctt agatccagcc tgaaataatc gtcgtatctc cgggaattat aagctccgtg
     2221 ttgaggttca aacgcgtagg cttctacaac gtaacgaccg ctttccacgg gggtatagcg
     2281 taatcctgtc cggtatattc cgctgcagga aaggctcagc aagtgtttac cggtataact
     2341 taaatacgct ttcagaaagc agggcaagtt gttttctgct ttatgccaag tgctgtctga
     2401 tttgatgtgg gcatcgagca aagtatacga aagagaggcc cggagtctat tccattcgta
     2461 ttccgccgag cattccaggc cccgtatatc tctttgcagt attttcattt tgtattccgg
     2521 accgtagtaa gtaggcgact cttcctgttt gccgtatatt gccgcctgaa taaaaagttg
     2581 ctctccctcg taggtgtact caagcgacaa ttgccgcgaa gtttggagcg agaaacgggt
     2641 ataacggtaa acgggataaa aacaggcctg gtagtttccc ccactgagca aaaaactgtg
     2701 tttttcttcc aatttctttc tgagatttac ttgatacgaa aggtaggctg gaagtttgtc
     2761 cgacaggatt ttgtttctta ggcccaatcc tagggtgatt tgtccgggca gttgaagttt
     2821 agcgtaaagc acggcttcgg gaataaagca ggcgatgctt gtatcctgat gcagcatggg
     2881 caccgagtcg gacatggaaa aagccagtgc gggtttgtcg gttttaaaat cgagagataa
     2941 atactcagcc gcaagaccag tttgtagcaa aagaaaatcg aggggaagaa atttcaggtt
     3001 gagggaagtt ttccattccc gttctttttg aaccgactgc aaaattccta acccaaagtc
     3061 ggagcggctt aaattattac ccagattaag ttccaggtaa gagcagtctt tgtgccagcc
     3121 aagattaaat acattgaatt gtctgatttt ttgagcatta ttgcttattt ctttgccaaa
     3181 aatataatct tcggcccgga aactttcgct gatgccgtag ctgtaaagat taataaaagc
     3241 agcctttttc ccccatctga aatttaggcc ggcatcgtcg gtgcggaaag attttatcct
     3301 cttaccaccc ggattgaaaa acaaataagg cccggaaaac tggtgattgg cgtagcattg
     3361 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggctgcc cctgaattcc
     3481 ggattggcat tttcggaagt attgctcgca gccggtaaaa aactgagggc ttttagggga
     3541 tcaccgtctg agatcggaga caaatagata tccagtttgt ttattttctg caaagaaaat
     3601 tcttcgctta gcctgggatt gtgttcaatc atggcagctg cgagcatacg gttgtcttcc
     3661 tgtagtctga gctctgtaaa agaggaaaga tcaagttctt tcagagggat gtttaaggat
     3721 ttgtatccca caaaaaacag gaacaggctg tcgtctgtgg ccgtatcggg tatatccaga
     3781 ctgaagtatc ctttggtgtc ggagtaacaa caactatttt cctgttgttt taaacaaaca
     3841 agaactgaaa aaaggccgtt tccctgttga tcgcataccc ggccctcgag caaagaacgg
     3901 gcctgaatac acaaggggaa aaagcataca aggatcagga taaaaccatg tttgaccata
     3961 aggggaatgt cattaaagcc gtaaccataa accaatacag gctgatgttg atacaggcaa
     4021 tcaaaaaata cagtaaatac ccacagcgtt tcagggatat tttttctgcg aaaggatgtt
     4081 ttacaaagcc cttgaagcac tcgtaagaat taccgaacaa gcgttccccg ccgatcaggt
     4141 cttcaaacac ataatagccg tcggtgccgg ggaaaaacgg attcagattg ctcaacaatt
     4201 gaaccgcata ataataaaac aagcaggctg taagcaaggc gaacagatcg ggcttgtggt
     4261 agtgggacag aatgatgagc aacaatagca tgatcatgac atccatcagg actccggcca
     4321 gactgagttt gatctttcgc cgtttgtcgt gccacaactg cacttgatca atgtgggtgt
     4381 acatgaccgg aaacaccaga aacatcagtc ccagacccat cttgctgctt tttcctccgt
     4441 aacgcatata acaaagataa tgtccggttt cgtggataaa cagacaggca aaaatactca
     4501 ctaccaaaag cagaaaaaat ccaaaaacag gtatgtgagt cattccgaaa ccggaaaatc
     4561 gtatttctgc ccccggcagc aaccacagta agctgatcaa caaaaccatc gtcaagatat
     4621 agagaggcag caaaatcttt tgcagccgtt cggaaagagc cattttatgc aagacggtgt
     4681 tgtggaggtc cagagagcat aatttgtaaa aacgcagatt acctttacac tctgcaatga
     4741 gatttagccc acagagtttt cctgtgattc tcaggaagcg gacaaaaagg aaataagctg
     4801 ttatttttaa acctgtactt ctctcaggag ttttcctgat gctgccctgc ctgatgagca
     4861 gacccaattg gcgagctttg ttggtgagcg cgtcgaataa gtctttttct atatggtaat
     4921 cttgcattaa actacagacc ttctcataat cgttgtcaat gctgtaataa tgtaaaaaaa
     4981 tatattctgc ttcggacagg cttaccaaag caccgttgct gtcgtttttc aggattaatt
     5041 ccgagttgtt gttatcgaga atcaaaaagt cttcgttcca ttgccaggtc gctgtttcca
     5101 tggttttgtc atttacctat aaacatactg agcaaagcac attcgtcggg ttgttgaaaa
     5161 cccaggaaat cgttcagatt ttgatcgatg taaccaccta tagcgcagca ggatactccg
     5221 ccggctgctg ccgacaaatg caaattctga caaagggccc ccacgtccat caatacgaag
     5281 cgcaaacccc ggtcaccgta tttgcaggaa gacctgttca acacccctcc gaaaacgacg
     5341 ataccggaaa tcaagtcgag attccaggaa cccagcaccg cttcgggaaa acattttctc
     5401 aattggcgaa agaaattttt tttatccccg gccggctgca ataattccaa ggcttcttcg
     5461 tggggattgt aaacaaaaac acctctttgt aagcctatgg tattcagatt gatataatac
     5521 aaatccaccg gataaagagc gccgcccgag ggaatgcttc tgcgcgacaa atggtggctg
     5581 tgtttctcga agcgttcggt aataaagtag gagttgagca ataagttggc caattcttcc
     5641 agattaatgt gttcatgaaa agaacgggtg cttttgcgct taatctgaat atttcgcaga
     5701 gcaatgcttt cttcgttaaa taaagtattc agcttgatcc tttcggtagt tcccctttcg
     5761 agcgtatgag caataatttg ccggctgtaa tagggaatgt tcattatact ttgaatccgt
     5821 tgggcatttt ccagctgatt aaaaggagtc atggaggaac tttgcataaa atccagtgcg
     5881 tattggctca gtccctggtc gtaaaaaaga ttggcaatat ccataactat tacttgaatt
     5941 tcaagattct gggatcgtcg gggtaaagct ctatggcctc ggcaaacaag tgcctggcct
     6001 tttccatgtc tttgcggcaa taatgatcca acaaataata ataggcgtcg tttttgcccc
     6061 aagagggatc gttgggattg ttgccgtatt tgtcgcggag tgaaatagcc ttcagaaaga
     6121 gctcctcgaa ttgggttttc cctccgtaaa cggccggcgt ataatagtcc ctgatgccca
     6181 aagccaggta agccctgagg ttttgaggat ccatgtcgat ggctttttga gcgtactttt
     6241 tggcttctcc gctcacaaat ggaattttta cgtttgcgac aaaagccacg ctgtaatttt
     6301 tgaggatggc taataaagcg tagtcctccg attctttgtt tttgatttta ttcagacact
     6361 tcagggcttc ggacaattca atacgggccg ttttctgaaa ttcttcggcc ggattggatt
     6421 gggcacacag cgattttttg taccggccat aggccgtcca gtaattcagg tagcgattgt
     6481 ttgagctttt gttctccaga tggagagcat tgataattcg gtcgatttct tccatcctgc
     6541 cggttttaaa ataatgaata aatgcggagt cgatttccgt ttgcaggttt tgtgcttgca
     6601 gaggtaatac tgcggctaac aaaagcagta aaaagagttg tttcatacaa ataagtttaa
     6661 gggaaggggt gaggataagc tgtaaataag gtctgattac cgttaaggca atagcctaaa
     6721 aaaggccagt tgtgattacc ggtcaacaga gaccatcccg gggttactac ccgcataatt
     6781 ttgtattgca gttcttctgc atctattggg ctgatgtctt tgtacataag gtgtttaagt
     6841 ccgacctttt tgagttcttc aacggtaaaa tccatgcatt tattatcggg atcggcacga
     6901 tagatttttt catcgcccga atcaaaacgt gccgcctctc tgagtatggg cgcttctttt
     6961 ctgaattggg ggtattgatt gtaaaaatgg aaatgcttgt cgaaatcgtc gatccggcta
     7021 agatccggtt cttccggtag taatttcttt tcattcaagc tgatggcgta ttcaacgccc
     7081 tgataagcct ccaggcaggc tttgatcaga gcctgagttt tgctgaaacg cgctgcacag
     7141 cccaaggatt ggtaaatctt gtttttatag cggaaataga taaagaccac catacattcc
     7201 acaggagaaa aagccgccag gtcaaaacat tttatttgaa cagccgaatt ttgaaagagc
     7261 cggcaaaccg ttttgttttt atggtaatgc ttcaaaatga cttctttcgt gtaagaacga
     7321 taaggcaaag catcctggtg ataccaaaac agggcaaagg catgtctttc ggcacattca
     7381 agaaaaccgc ttatcaacgc gtcccggctt gttttgccgg ctgcggttcc ggtggaggtg
     7441 gttcctacca tataattgtg agggctgttt accttgctaa agtagggcat gtatatgcta
     7501 aaagctggca tccagtacga ctttcccgtg ataaaatcgt tgcagacagt ccattcgata
     7561 agatcgtttt cgcccaaagg gtacacattc aattcccgga gcctttcgta ttgctcgtcg
     7621 gaataatagc gaaagaattc cggagccagg gccggttcgc ttttggaaag ttcggaaaag
     7681 ctggatactt tgaaatcttc ggaatcataa gtggcacagg cataacgttc caaaaattct
     7741 cccaaggccc cgcgatgact ttgttcttcg tcaaaagcta ttgagctgcc ctggatctcc
     7801 cggcggcggc agtttatcca gtccaggttt ttacccgata ctacgctctg caccagaccg
     7861 tccggtatga aacgacgatt gggtattctt atcatggctt cgctcagcaa gcccgctgca
     7921 gtatgacata tcgttctcat tgtcaggcat taaaggggct gaaggccggt cttttgaaca
     7981 aggacgaagc cggatcttta agaatttgtg tttcttcggt tttgtattgc agaaaatcaa
     8041 tagtataaga ttttcccatc aggtgggttg ataatttacc tttgaaactc cagaaacgct
     8101 ccagttcgat tgcgatcagt tgcatgcctg catggtaatg agcataggag cgtatatcga
     8161 cgttatgaaa acgttgtttt tcttcggaca ggataaattc cgaataaaga gccaagctgg
     8221 acaagttgac ggccttgcgt ttcatgtaac aaccgagaga aggcgtattc atagccggga
     8281 cgaccagtgg tcctaaagta agagtgctgg gcgagaattc gacgtacaga acgggtatca
     8341 tgcttttgta acttcggtat gaaagatcct gcaagagcga atagtgatca aaaacaggac
     8401 ttatggtcaa aataagatgc agcccttgca cactattatt ctcgtataca ggattaaaac
     8461 tgagttcgaa ctgctccgtt ttgaggtcgg atcctaagtg cttgaataca tttttgttcc
     8521 cgtagaatcc gattttcttc agttgctttt tttgttcaca ctctttgtat atcagattgt
     8581 tgcttagtag aaattttacg atttctgaaa attctgttct actgacaaat tccgattgga
     8641 aatcttccag tgaggcgaca ctgcggagat gtttcaaaac ctctattatt ttatggttgg
     8701 ccttactttg gatttttatc agatttccgt cggagcgttt gcagtagatg gcgtctctgc
     8761 attcccagat gtccatattt tgtattttga ttttcatggt attgagtatt ttggctgttt
     8821 aggagagccg cccataagca tgagccctga gcgcctctcc ctatactagg cttgaatcaa
     8881 aaacaacaga ttggttaaac ctaaatgttc ctgcttgtac ttaaggaatt accaagatct
     8941 cccggcgtgg ttaccgtgga acttccacca gtgccctcgc cggtgccgaa gcagcaagaa
     9001 cagcaacagg tgcagcaaca cattccggga aggttgcgtt cggtcagcat tagaggagcg
     9061 gagcgatctt ccactacctt catggtcatt tcaagtgttt tcatgatgta taaaatttat
     9121 gtgataaggc ctactcttta ttggcttttc ggctatctcc aggattaatc caatgcaaat
     9181 taaaatcaaa taaatcatta atcaaaatat aatagcgaat ttaacacaac aaaataaatt
     9241 atttaacatt aaacatatgg gcgcaaaaaa accggagcta taaactccgg ttcatatatt
     9301 agtaaataac ctattacgca ttgataaata ccctattacg cagtaagctt tgtcaaaatc
     9361 tcagtcaagc cctgaggatc gggagagtaa tagtcggcgc cgatttgttt gcgaaactca
     9421 tcgtttaccg gggctcctcc cacaaaggtg gtggtttggg gcgaagcctg cttgatggct
     9481 ttgtttattt cttccatact ggccatggtg gtggtcaata gggcagacat gcccacatag
     9541 gcgcctggat gggcctggat ggcttcgata aatttctcgg ccggaacgtc aactcccagg
     9601 tcgatcacct cgaagccgct gccctcgacc atcatgctta ccaaattctt accgatatcg
     9661 tgcaggtcgc cttttaccgt acctatgata aatttaccct tgcgctttac ggcgccggag
     9721 ttgaaatagg gcttcaggtg gttcatggcg gtactcatag ctctggcaga catcagcacc
     9781 tggggcacaa acactttgtt ttccctgaat ttaactccga tttttcccat acccactatc
     9841 aatcctttgg tcaggacgtc gctgggagaa actccctgct ccaaagcccg tttggtgtat
     9901 tcgtcggcgc cctcctggcc cctcatgtcg ggaggatagg gcgaggctaa atttatttta
     9961 ccgtattcaa cgcaataagc gattttgtct aaaagttgtt ccatgttgtt attattaaaa
    10021 taaggtccga ccgcagactg caaattaaat caattttcgg gggctttaca aacgatattc
    10081 ccggcgcaat tgttgggctg tggaaaatat ggtgtgaatt ttatcgtccg ttaccttatc
    10141 gtccatattg atgcagcaga tacccgtcag ccaaggattg gcgcaatcgt tgaccagtct
    10201 gcggatatgt tgttcgaggg tttcgcagct catggtattg atgcttaccg gatcgagccg
    10261 gatattgata aaggtagagg gcaaatgttt acgcagacag accatatctc caccccagcc
    10321 ggcatccaaa aagtcgagat gaggaatttt tgccatttgg gccgcgtgcc tgtggggatc
    10381 gggaccgcaa taatgcacac cgtagggacg gtatttcttg ctccattcca catcgaaggg
    10441 aagcagaaat tcttcgtatt gttcctccga aatcatggta tgcgagcatt ccgaatgcag
    10501 gtaaacggcc ggtgtgatgt tgatcacgtt ccggtttacc gaaatggaat tactgccggt
    10561 ttcttgttgg ataaagcaga aaaaacgctc aatcagctct gcgatttttt tgaaatacag
    10621 tttcacttcg tcgggttgca gcaagaagtc cataagtatt tcttcgcccc ttaaatccat
    10681 agccaggttt aagatgcctc cccaattcac atcgcctttg agatatccgt aacggctttt
    10741 gagttccgac atcagttttt cgagtctctt aaaggccgga ccggcaaaag cagcatcgac
    10801 atctacatga aaatctgttt tttgagcggg aattacctgg ggtgcgctgt cggcaaaata
    10861 gcgaatttca cagcccagca tttcagaaag taaataaccg gctgcgttgt gaacggctcc
    10921 tattagtggt aaatctttgt cgtaatcgct tcccagtccg aattgcccga agcgttcgta
    10981 caattctttt tccatgcggc gctcagattc tactctttta agcggatggt aaaagaagtc
    11041 ttcgtcaaaa tcgatgccgc aatgcttatg ccaccaggag ggatgaaaaa cgatttccac
    11101 cggtaacggt tcgttgctga aagcagggtt cttgatataa gtcatggctt tagttttaat
    11161 ggcttcaaat gtactgtttt tttgccggtc cgaatttttt gcctttccga aagtccggcc
    11221 ggctttgtat tatcttatcc ggatctgcag gctgcccttt tgcggcccgc tcagccattt
    11281 ggcttgcgag gaggggccaa ccagatcggt cgaattgact ttgttgcgca cacctggtat
    11341 aacgtcaagc acagcaattc cactttgagg aatggtgtac agcagggcgt cgcgtccgtc
    11401 gcggggcgta taaacgccca ggaagctgcc cggcgtgccg tttcctaacc agagctgagc
    11461 ctgagcggtt tggaaatcta cccatttcca gtcgttgtaa tagcctttga attcgggata
    11521 aataaaactt tcgcccggaa tggggtcgtt gtattctttg tcccacacat cgaaaagggt
    11581 gccgtgcata cggttttgcc acgagcgata ggggcctttg cccaacagcc gcatgccctg
    11641 catgttatct tcgggataat cgaagcaaat gcctgccagc tcaaccactc cttcgtattg
    11701 atattcgtaa tcgacggtga tttcttcccg gtcggtgatg gtccagcaaa cgctgttcag
    11761 gttgccgaag tattgggttt tgaacagtat tttgtcttct tgttcctcta cttctaaatc
    11821 ggtcagcctg gagttgccgg agatatcggc gtaaattctg tccttgcttt tagctgcttt
    11881 gtcgtcgtgg ttgtaaaata cgtccataga gcggtcgctg cgacgtgcgg cgataaaacg
    11941 cggcccgccc gaaagagata gattcttgtt tcctttactg atttggatga ggtagcccgt
    12001 ttccgtatcg aattgtaaac gcgtagctcc tgattctatg gttagggtac ttccttcgcg
    12061 gctttggctt aatttggcag gagaggtgtt tgcttgtccg gctaaagctg cccgggtgta
    12121 atcccggtcg cttttccagt accagcccga ggtgtaaatt tcttcgcccc ggggattgag
    12181 cacgcttaca tacaaaacat cggcctcttt ccaattggcc ggcagtttaa gctgaaattc
    12241 gccgctttga tgcggggcca gtttgggccc ggcttgttcg ccctggccta taatgttgtg
    12301 acctccgctt ttatcgcccg gtttggcaaa attggccaaa gaccatttta tgcggcaatc
    12361 ttcgagggaa ataaaatcgt agcggttttc tacatggaat tttcccacaa aagcttccgg
    12421 atcggcaata aagctgtttt gatcctgcct cagggcttcc gtccgctccg tgcaggtgac
    12481 ggttctgccc agagccttga tttgtaccgg acaccagact tctttgacgg taaagaaact
    12541 accttctttt tcgtgatgag gccccacgat gccgtcggcg gcgtagttac ccacattgtc
    12601 gatgcggccg ttctggtcgg tgcgcatcag gccctcgtcg gcaaacaccc agagaaaggc
    12661 tccggcgcag cggggatgtc ggcgcatcat tacccagtaa tcccaaagtc cggcgccgtg
    12721 gccgccgtcg tagaggccgt gcaggatttc ggtgggcata aagatttcgg gtttgcgcat
    12781 gtattcctgc gattcgccat aagaacggta gtgcatggtc tcgaaaccgc cgaagttacc
    12841 ctgggggtga agcaccgggc gtttttgcgg atcccaggga tgaaattcgc tgtcgagctc
    12901 cgtgttccag cccccctcat tgccgttgga ccaccagatg atgcttggat ggttcaggtc
    12961 gcgggtaacc atctctttta ccagttgttt ccccacttcg gtgtcgtact tgccgtgcca
    13021 gccgccgagt tcgttcatta cgtaaagtcc cagctcatcg caggcatcca gaaaagcagg
    13081 atcgggggga taatgcgaca aacggacggc gttcaggttc atttcgcgca taagacgcac
    13141 atcgttgtaa ttgtcctctc tacttagggt tctgccggtt tcggggcgga aactgtggcg
    13201 attaacgccc ttgatcatta ctttttgacc gttgatgtat aaaccgtcgc tctctttgac
    13261 ttcgatggtc ctgaatccta tcctttgttc tttgactata tgaacggcct ccttgctttt
    13321 gagcaggctg aattgcacat cgtacaggtg gggtgtttcg gccgtccagg tttttatgtc
    13381 cgtgaattgc cctttcacaa tgaccttgtc tgaacccggc cttaattcgg ctttcagggc
    13441 tttgccgatg tttttgcctt cccggtcgag tagttgcgct tcaatgctgt agtcgccgtc
    13501 cagggcagta ctcaaaaata cttcggcgta aaaagagccg tcggctttgg cgtcaatggc
    13561 tgtacgatcg atgctttgcg caggcagggc ctgtataaat accggacgga agatgcctcc
    13621 gaaattccag tagtcggccc tgcgttcagc caggttgacg ctgctgtttt cggattcttt
    13681 gctcacggtg acttcgagca ggttttcctt gtcgccaaag aatacccggt cgctgacatc
    13741 ggcataaaaa cgataaaaag ctccctgatg cagtttgacg gcacggcgtt tgtttatttc
    13801 tgcggagcag tcggtcatca ccccttcaaa aacgaggcgt attatgcggc cttcccattc
    13861 ggccggcaat ttaaaccggg ttttatatag gcctttttcg ttggcaatgc cgggaggatt
    13921 ggcttttccg taaaaaggca tcccgtattg atagatacca aagccctctt gctcccagca
    13981 ggaaggaact ttgattttgg tccagctgcc gctgtttcgn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnc ctcttgctcc cagcaggaag gaactttgat tttggtccag
    14161 ctgccgctgt ttcgacctcc ggtacacata aagtcccatt cgacggcatc gtccgggcca
    14221 tgaccggaca gatagatttc ggctgtttgt aagggatcga agccgggggt cgaactttgt
    14281 tttttaaagg cggaaatttc ggcgacactg ccgcaaagca gcattatcaa ggagaatgtt
    14341 ataaggtttt tagctttcat aaagtgtttt gtatagtgtt taaggggata gaatctttag
    14401 agggatagta tcagtaaaac aaccagtcga tggctgtttt ttccataaaa ccggcgtctc
    14461 tcgtgctaaa taccgcttcg ggcttggaga aacccagcac caggcaaagt cctttaccca
    14521 ggttcagatt gtgttcgccg gcttcgaaac ggtattgatg cacattcact gccggcagat
    14581 tttctatttc catggcgtta gcaattttga tgtccgcctg accgtattga ttggcgctgg
    14641 catcggtctc cagacgggga gcgggcatat agtctccttg cctgaaaggc ttgaaataag
    14701 caacccaaag gtcaacggct tcccgactgt tgaagcgcag gcggatgttt tcattgagtt
    14761 gttggcggtt gctcatgcga agagccttga gttgactgag ttccggagcc agggctttta
    14821 tcttgaaagg ccggtcgctg tacacgcttg ctccttcctg aagctgataa aattccgccc
    14881 gggcaggaga aataagtttg acttcggccg gactcagcac tttcaggttt tccggggtct
    14941 gattgcccga tttgagtttt tgaatattgg catcgaaacg cgccagttct tgttcgaact
    15001 gaggcaataa ttccgtccag gtcttgttgg ctccgttgtt gccggtgacg ggaatgcggc
    15061 gcataccggt ttgcatgctg ttggcgtaga gatagctgtc gcgggtgagc tcttcgagtt
    15121 tgcggtagtg atccaaactg ctttccatca aactgtaagc ttgttccagg tcggaaattt
    15181 cgttggaata ggaatacctc aaaacgagca tggaggcttc cagcttatcg gtaaagcagt
    15241 ctgtaaaagc cttgtaggca taaatgtcgt ttttgagacg tgcaaattct tctttgtttt
    15301 tgcgcacacg ggtcactttg tcgatggctt ccaccgcttt ccgggcatgt tcccgtgttt
    15361 ctgctatcag ttggggagga gtttcgccca catgtgcttc tccatgccat tcgcggtagg
    15421 cccaaacttc gagtttctcg ccctcggggc cgcaggaatt ggcaaaatcg ggatagaccg
    15481 tccagcggtt ggggttgacc aattgactca taaacattcc cagcaggaag gtttgccggt
    15541 tgccctcggt gataccgaat ttgcgcagca atttgggcga tatttctcca ctttgttcgt
    15601 aggcctccaa aatgtacttt ccttgtttgc cgcagtcata aagttcggat aataagttcg
    15661 accaataatc gatttcttcc ttacggtcgc gatcggccga ccaggcataa cgcgcccagg
    15721 attgatacca gatccagtcg cggtccattt cgagcagacg gctgccgtct tcgattttgt
    15781 ctgccgcata aggccagtcc cagtatgagg cctggggata gaggtgcaaa ccgttggctc
    15841 cgtgtatgtt gtgcatagct tgaatgctct tttgtataaa gtcgggcgag ccgtagcgga
    15901 aaggttccag gttggccaaa atatggacat tggagatatg aacagatccc agagcgctga
    15961 gtgcctgatg tgtagccgtc caggggcctc tgggttgata ggtgcatagg gattcaccgt
    16021 tgtatttgtt catggtgtag agattcttgt aaatgggcaa ggctgcatcc atgaccattt
    16081 cggcgttggt atcgtgcgcc ctgagcacaa taggcggttc ctgctccaat ccgagagctt
    16141 tcagcccgtc tttcacaccg ggaataatgg tttcggtaaa ccattccaca tcgtcttcgt
    16201 gggtgttcat ggcttcgccc aggcagacca gtaaacctac gttggggtat ttttcgataa
    16261 aagccgcgac agatttacgg gtgtaatccg acagcaaagg agtaatttcg actttgcggt
    16321 cttgagtggg tattttgtga taatctgcaa agggttgaga tacaataata ttgtaaaaca
    16381 tctgaatcac ccaaatgccc cgtttgttgg cctcctgagc aagaaagccg aacatttctt
    16441 cgtttcgctc gaaatcttct tcgctcactt ccacggcata aggataatcg ggaagtttaa
    16501 ccagcgaggc aaagggatgc ccgttccaga gataaacgga attcatgcga ttctctacca
    16561 gcatatccag atacttgatc cagagttctt tatcgtaaaa ccaggggaag agctcctggg
    16621 tatagggata ttcgtaaacc ttacgacccg gcagcaagta cattttttgc ataccgatgc
    16681 aagttcccct caacaccata tcgggagcat cggaataatc tattttctta ggcagccgtc
    16741 cgtttgtttt caaacgatcg atcagttcca tgcatccgta aagcaagccg ctttcgtcgg
    16801 ctccgcagac cgctatttta tctccgtctg caactatcct gaatgcttct ttgccttcga
    16861 cgggttctat ggcaaggccc tgttcttgta aggtctgttg aaaattcaga tcattctggc
    16921 agccgagata tatcttggtc gccttgccgg cagggttttc atcaacaata gtataaccat
    16981 tggaaagcaa actgtttgat aatctttctt ttgcgtaatt acctctagga gaatccgacc
    17041 ctgtcgtcag cagttgaact ttgggttgac aggcagaaaa cactaagagc aaagcaaaac
    17101 tcaggagata atagggagtt ttcatgaaga ataggtttaa acgtttcaga attacaaaag
    17161 taggattatt tcagtatcgt aaaactaaaa aaatcaaaaa cttgcgctgt agggcttgct
    17221 tttataaaag aaaaactcag caaaataaaa aagcaagatt gatatcttac tgagatatct
    17281 atcaatcctg ctttaaaatt tgaacttaac gaatatccct attttgccgg gctaaaagaa
    17341 acaacgctat tattttacgt atttccggat ggtttttaat acaatcggtt ccatgatggc
    17401 gtaaccttgc aagttgggat gcacgccatc ggcagcaatt tcaacaggta atcctccacg
    17461 ttcatctact aaggccgaat agtaatcgac gaaaggaatc ttattggcat cggcatatgc
    17521 cttaatcatt tcattcagca atttgatatc ggcagccgga gtcagttcct tgttccatcc
    17581 gaattcgtga gcaggtaata tggagcacaa taccggtttg attttgtgaa actgagccag
    17641 ttcacacatc gatttgatat tttgtacgat atgttcatgg ctgatgatgc cattattacg
    17701 cgcaatgtcg ttggttccgg ccatgataac aaccatcttg ggtttcaggt cgatgacatc
    17761 agcctgaaaa cggacaagca tgtggctggt tgtttgtccg cttattccac gaccggtaaa
    17821 tccgttttcg ataaaaaatt ccggatgtcg cctataccag ccttcagtaa ttgaatttcc
    17881 cagaaaaacc accttggcag gtttctgtat cgaatcgttc atcgcctcgt accggtaaaa
    17941 gcgcgcccaa tcggttgatc gcatttttgc tttttctttc tcggcaaaat gttgttcgag
    18001 ctgtttgtac tgatccgaat cgagtgtgat actgatagat tcagtgtttt gaccatgcaa
    18061 atgcaaacaa ctgaaaacga ccaaaaatgt aagtatgaga ttctttttca tttggtttta
    18121 aattgaatgt tcctgactta taaacgtaga tattttaaat tatgtatttt atcgttctca
    18181 aaaactcgta ttatatagat acctggaatt aaagagccgg tatctataat gcttgtatga
    18241 tttgttttct gacataattt tccggataca tcgtaaaaat gcgcgtattg ccagtttccc
    18301 gatatctgta tttgattgta acgatgatag atcagcctca cagtagacag attctctttt
    18361 acagccggaa tgcttgtgtt gtccgataag cttggtggaa ttaattccgt tgttcctgcc
    18421 ggtaacttat aataaaaatg cgcactgaaa ttaaacaggg gatacggttt cccataaata
    18481 acaccgtttt cgtcgcgagg ttcgtatcgt ggatgtaatg acgtgttgcc ggcgtcgtgt
    18541 tcaaaaatgt attcaccatt ttcgtctttg ccatccaaat cgacgtaggt atagatggat
    18601 tggtaggggt cactatcacg aagattccca tgtcccaata catcctgtcc ggcataggca
    18661 atttcaatat ctccgttaag ttcgtcaaaa caagaaatta aaacactttc gccattttcg
    18721 tgtaaagtga cggagtttat gtttaccaac gaaccattat tttttatagc gaaaccgaaa
    18781 tccttctcaa aagcaaccaa tagggtgtct aaaacaagtg gtttgcaggg cacaagataa
    18841 tcaatccgca atgtatgatt attttctttg gtaattttcg aaggctgcaa gggcataaat
    18901 gtttcttttt caatataagt tttataaaaa accttggcca acatttcccc gaaccaccgg
    18961 taaccatttg ggtctaaatg acccgcactc cggtcggtca tcaaatatac aggtccggca
    19021 catacgatat ctgcttcctg attggaagct tcgagttgag ccatcgctat gttttgttca
    19081 aatcccttta tgtactttcc tccaacctga taagtaataa acaagggttt ctcctgttga
    19141 ccgtataggc tcataatatc cgattgcata tcgttcttta gtatttgcat cagatctctg
    19201 tatcccgact tatctgttgt actaccatta agggatatta aaccgggttg atttcgtgag
    19261 taatttgttt ctccctgcat ccaaaaaata gccggacatt cgatgttgta attgtttctt
    19321 ttaacgaaag aagcagcact gttgatgctc cttataaaat cacgatgata ataatagggc
    19381 gatcgttggc agtgtttgga gagttcttcg attgttttac ctccggtggc acaagaagtg
    19441 gctaaaattg acaccggtcg ggtaagtttg tgttgtatat gattggcggc gccaagaacg
    19501 gagttttcac attttgcccc gtctgtccac gatcttgcga agttccgtat gacatcgtcc
    19561 ggggtgggag atgaaaacaa aggagcaatt tgactacaat tcacattacc gttgttgata
    19621 catacattgc ttcctatcat ataattgttg ggaaccggct ccgatgttat tggaggatac
    19681 gattggtacc cctcagccaa actttggccg taaatgataa agtgccaaaa attaggatga
    19741 atgggtcgtg taaaaactga atcttgatat gcaccaatgg caatgttgct ggttaatctg
    19801 tcctttcccc gttgatcttt aagaatcaca aaaccgggca agccgtattt gctcgccaac
    19861 agggtcattt cggaaagtgt caatcctttg gtattgacag ggttcgtttc gggatgaagt
    19921 attttacatg ttggggtaaa acctccgttg tctttaagag aatccaacat gatatccgca
    19981 tcaaaagata tttggccatc cggttctcca tcgacgttgt aatattgtgt gtcaaaaatc
    20041 gattggtaaa acctggatgt cgatgttgtt ccggccacta cttcgatttc gacaccattt
    20101 ccggataaga ttgaattata acagtttaga atgccgtttc ctcccattat aaatccgccg
    20161 gcactgttgt gagcactatt tcgggtaatg gttgaacttg ttgcctcaat tcgagagttg
    20221 tcactaacac cacctgcacc tccactctca aaggctgtat tttccgcaat tgtactatta
    20281 gccattgaca taaaggtgtt ttcaagtaag attcctccat tcgtttttgc ttcgttgtgt
    20341 cctatcgtaa cattgtgcaa ataaacggta gatgaagcgg ctgctaaaac accacccgca
    20401 ctattgttaa ccgtatttcc ggaaatgagt ccctgattga cgtaaccgtt ggaaccaacc
    20461 aagtaaatgc ctcctccatc ggtttctgca aaattatctg aaacgattac ctgctccata
    20521 ttaaaggaaa cccggttcag ataaattccc cctgctttcc ctctggaagt ggaattgccg
    20581 atgatttttg aattccgaac attcagtgtt gcgttctgaa tccagcatcc ggcaccgtca
    20641 aaggctttat tgtttgtgaa aacacaatcg gagatatcaa tatttccttt ttcggcaata
    20701 agtccgcctc caacaccttg ttggtaaaca atattgtcga tggttgttct gcgcgtatta
    20761 tcagccactc catcacgaat aatcaggccc gataatttca ccggcctgtt ccttctcgat
    20821 gcggcagtta tcaccactac atggaaagca ttcagctcag agcccaactg accgctcaat
    20881 attgtgggat gtatggcggg ttccggtttg acatgaccgg aagcatcggc gggataacct
    20941 ccaataagag caatgttgtt tttaatttca aaacattttt ccttctcaac cctaccattg
    21001 gttaataaaa tttgcggtat gtatgttccc gctgcaatat gaatagaatc accctccgat
    21061 gaacgggcca gcgcgtcatc aagtgaaatc gcactttccc agtttacacc gctgttctcc
    21121 gaagacgtac ctgttgcctt gacataccaa ttattttgtg caagaataac agcttgtatc
    21181 tgtaaaaaac ataaaaacaa aacaaatttt cctgttttca taaagctgtg tgtttaaatg
    21241 atctgcaaat aagcaattgt gtttatgtac attaatcgat tctaatactc gaagatagtc
    21301 atgatcgggt agtgatctga aatataggga actccgtaat cttttacgat ggtttgatac
    21361 tgaattggtg tagcagaacg ataaaagatg tagtcaatga ttctgtcttt ctgtgtaact
    21421 ctgaatccat ttatggtacc cagcgaatcg gttttcgcag ctgttgattg ggatgactta
    21481 aggtattgat agatcggatc aagcagtgga tcgtcaggaa gggtattaaa gtcccccaca
    21541 acaaaggtca tcagtgtatc tcccgacagc tcagccgctt tttgaactat caattttgcc
    21601 gattcaatgc gtgccttaac tccgagatgg tcaaaatgag tgttcaggac aacaaattct
    21661 ttgttgtcgg ccttgttttt aagatgcgcc caggtcacta tacgattgaa tctggcatcc
    21721 cagcctatag aagaaacttc gggtgtttcg ctcaaccagt ttgttcccga acttaacaag
    21781 tcaaatttac cggtattgta aaacaatgcc gtgtattcat tgttttttgc tgaactatcg
    21841 cgtccttcac caacaaaagc aaaatcagcc aatgccgaat ccaggaagtt aacctgatgt
    21901 atgaggcctt cctgaatacc aaaaacatcg ggttgttgat cgttgatcat atccacacaa
    21961 gcctgacggc ggaaattcca gttgttttca ccatctttag gttcatcgta acggatatta
    22021 aacgacataa tacgtaaagt cggtctgttt tccgttttgg cacagccaaa caaaaatggg
    22081 atggccatga ttgctatgaa taatcttttc attataacgc cgttttattg ttcaaaatgt
    22141 tgatttaata attgtttgtc ggagttgggg taataaccta cttaatggtt aaatccacgt
    22201 acaccggata gtggtcggag gggaataaat tgttttcata cgcttcatca atcgcccggt
    22261 aagtgtgtat tcggatttcg ggcgtgacaa agatgtaatc gatctgcacc ggatgcacac
    22321 caccaaaatt atgtgccgtt ccaaccggac catacaccct ttcctgcggc gccgattttc
    22381 ttgagtccac aaagttctcc gacaaaatcc ggtaggcttc tgtattcgat ttactgttca
    22441 ggtcaccccc gattatgaaa ggagtattgc ctgcaatcct ttttgcctct ttgctgataa
    22501 gtgttgcgct gttggtttta gccatcagcc cccgatgatc gaaatgggta ttaaagaagt
    22561 agaattcttt tccggtcttt ttatctttaa attttaccca agtgcaataa cgggcatatt
    22621 tcccgtccca gcctattacc tccggctcgt tgttttcgtt taaccagaac atattccatt
    22681 caatgactcc gaagcgcttg ccgttgtaaa taaccggatt taataagccc tcttttccta
    22741 cagactcata agaggttccg gccacaatat cttccagttg aaaaggaaca gcttcctgga
    22801 gtcccacaat ttccatgttg ttctccagaa taactttgat taacgaatct ttcctgtaca
    22861 tccatagatt tactccgtcg ttgggatctt ctccgcggat gttgtatact gccaggcgga
    22921 tattgtcttt agggaattta taatcagcag gtttacatcc aaataacatc aaaccaaaag
    22981 tgctgatgca tacaaataac aacgtcaaat tctttatggg tgttttcatt tacattttat
    23041 tttataatac tagtttctta gctgtgttac cgacacgcac aataaaaact ccgctatcga
    23101 ttatgccggt caaatccagt atattggctt gggtggtttt ttgaaagata acttcaccgg
    23161 tgaggtcgta aatggttacg tagttttcgt gccggtttcc ggtgaagaat attttttttt
    23221 caatattgta aaatgtactg cgatcttcga tttgcttacc ggtgctgttt gtttcgtcca
    23281 gaatggcttt gacaacgagg ttgtcgatag caagcccatg atcgtttccg cgatcgtcaa
    23341 tatcttccca acggagtaaa accacttgtc ccggggcaat tggtgcgtca attttacctt
    23401 tgatgcgaac acggttggct gcattatttc catcaatagc aacatcggtc aatcccgatt
    23461 gtgccgccgt attgttaacc ggagaaacga acgacaaaga atctacggtt gtggttgcaa
    23521 cctccgaaat ccataatctt ctctcacgga tggctttggc gtcgggatta acagcatagg
    23581 agaagctgag tttttgctga tttgcaggat tagttccggc acgccacatc tctccgtcgt
    23641 aatcgatttc cagtccgctt aaaaattcac cggtgttatt ttcaattact actccgaaat
    23701 ggatattacg gtggtacgaa gagatgagag aacccagggc acgttctgct gaatgttcgt
    23761 tgggaactac cggatttcct tcgccgtatg aatagatcca tcctgcatct tgatccccgt
    23821 tgttgatgcg gtaatagttg aacgtagacc agtctgggat gatgcttcct gaaggacgga
    23881 cattaccgga agtagtaaca taccaacctt tggggctatg tccgggaaca aaggtcccct
    23941 tggccgttct gtccgtcaac aggtgattgg ggtaaaaggg cagtgtgttg aaatcctgac
    24001 ggtaaatata gtcgtcctct tctttatcaa aggaggcaac cataatattg cgcaacagtt
    24061 tttttgtata cacatttccg gctcgatcgg taacttcgac gataaattcg gcatcgggat
    24121 ctgtaggttt gtatgagaat atgtgggaag tggcagaagg tcccggattc tgagaaactg
    24181 tggttccgcc atcgcttccg ggatagttgg gacgggtgtc tcctactttg ccaaggaagt
    24241 cgtacatacg ggggtcgaca ccggtgaatt gttgcattac tttcttcaca ccgttttcgt
    24301 agatgtttac actccaccgg ctatcgtaat tccagacatt ggctatgata caatcgcctt
    24361 taccgttgct ttcgtcgaaa cttccggtag gataggcctc aaattgataa tctttgcttt
    24421 tcccggtagc tttgtaatac cagttattga atcccctgtc tccggcttca aagactccat
    24481 aaccgtttgg agtaccatcg gaacaatagt cacctgacca cgcagctcca tttgcagaac
    24541 cgacaacgtg ttcaaaaagt ctggcactaa cttcattatt tgttaaacga tggtggtggc
    24601 cggcaatgat gcgttgaatg tttctgcgac ctcccatcag gttgtacact gtttgtctgt
    24661 tagctacatt tgatccttga attggggcgt gtaccgagat tacaatggtc ttttcgatag
    24721 gtacctccgc aagatcttta atcaaccaat caatctgatt ttgcgtaata ttgtgcgtgt
    24781 actcttttga gttgcaacca atgtaatcga tatcgtccat catcacataa tggatatttc
    24841 ccctgttgaa agagtagtaa gtaggaccaa acgcggcttt aaagtagtgg tcagactcga
    24901 tgtcaccaca tttagtcata tcatgatcat gattacctat tacattgaaa acggggaaac
    24961 cggtacctgc cacagaagct ttgtgctctt cgaggcgtcc ataatcgtcc caaaccaagt
    25021 cgcctacaac cacaccgtaa aatggggttc cggcagggta ggacgctttt aattctttaa
    25081 tgtctttgat tgtttcattt ctgaaacgcc atgtttcata gtcccttcta acttgaggat
    25141 ctgcgatggc tatcataaca tggttaaaat cggctgcacc tccatcggta aaggggacta
    25201 aggtaaaatt gtgagaaaaa ttgcctgatg tcgaaactcg ggcataaatt tgaggtactc
    25261 cgtcgtctcc catgggaatt tcatacgctt cgggaacaga gataaaaaca aattcggtac
    25321 ggtcgtgtcg aggaaaagaa taatagccat cgggacctgt tttaacaacg gcgtacccgt
    25381 cgctgataac agcattggga ataggatctc ccgagtctgt tttggcaaaa cctgtaatgc
    25441 tgtcggcttg tgtggcggaa gcattagctg taaatacaaa taaacctata agaagaaaat
    25501 agaatacctt tctcatatat cttttttgct aataccggat tcaagaaaag aaaaatggaa
    25561 ctgtcttaaa acaataattc gaccgaaaaa cgataaggtg tcttagaaat gcccaaatga
    25621 tgtgctttag atattcgact cagcctcata ctcaagtatg ctccttcgcc ctcaatctca
    25681 gcacctcgca tttggacact tccaagatac cgacagaaaa tgatttattt aacgataaat
    25741 ttagctgttt ccacagctcc ggtcattgat tttagttgtg cgtgatacaa cccggcaggt
    25801 aagttattta catctatact gattgggttg ctgtattttc caattaactg accaacctgg
    25861 ttgtagagat gaactacata atttgtttcc gaagtttgaa tattcagtac attggaagta
    25921 acggtgggat agaatgtaac atttccaacc aacggatttt tcaccgagga tccttccttt
    25981 gcattcagag gcatgtaacg tacattacga ctcaacccgc ggatagattc accatttgga
    26041 tcttgggcgt aatatgtaat gggtcccgga ttggcagtgc tatcggttgc aatcacgcca
    26101 aaacacagat tttcagccgt tgagatcgtt ccaatcaatg cattattata aaagttgagt
    26161 tcttcgggaa tcaacaactg atagaattgt tgctttccgg ccggagacgc aatggacaat
    26221 ggttgattat attcttctcc tactgtgaat tcgggcaatt gattcggaac tatttccggt
    26281 aatttatact tacccaataa ggttgaagta aatcctacat aggtattggt gtatttgttt
    26341 tcaaccggat cttcggggca atatacctgt aagagagtgt acatttcttt acgttccggg
    26401 aagttgttat ggaggtcggt aaaactatcg aacatgttgt cgggacaagt ataaataacg
    26461 ttagctcttg ctctgatttc caaaagcata tacacgtggt ttttatccgg acttaaagca
    26521 atgtctttga ttacttcagt ttgggttcca ccgggacgtg caacggaggc cagtttgccg
    26581 tcgcgtgtga gcacgaaata aagtccatca cccccaccat ttgtattgtg ttgcatccat
    26641 ataccgtcaa cggttctgag gccggacctc gcgttcccac caaagtttga atgtgagtac
    26701 aaatctccaa agcgagatga catagtacca acgacatacc atacaccttc atcggttaca
    26761 cgaatgctgc tgccgacagc tccgccacca ccaacagtag agccttgtcc aaatgaatac
    26821 caccagcggt aatctccatt gtctttgtcg aatgtgaata taccgatgtg atgtgctttg
    26881 gcctcgccct cttttgttgc aaatccattt tgcagggtgt cggtactttg gtcgattgta
    26941 atgtcttccg aatagtaggt gctcattaca gcgacttcac cgattggact gatttcaatt
    27001 cctgttactg catcgctgcg tgaacctttg atatgtctta cccagctttc ttctcccaag
    27061 ggagtgtact tcagtaaaaa taagtcgttg atagccgttt gagtcgaaag attggttccg
    27121 gccagtgtag ttgatcctgt gaaagcacca cttacaaaaa tattaccatc ggcatctgct
    27181 ttagagaatg cgtttacaaa gctggcttcg gccgtttgct gatattttac ccagattaag
    27241 gcaccatctt tggaatactt agccacatac atctgattcc aatactgggt atttcctaca
    27301 aagccgttca ggttagcggg gtcaaaagac atttcactac caaaagtacc tgaaataatg
    27361 atgttgccgg aaccatctgc agttacactt tccacagtag cttcctcggc tggtaattgc
    27421 cacattttga gcacgttgcc atttgaatcg taacgaatta caaagatgga aagggcagca
    27481 taaccggaag gatcttccat ggccattgaa tcattttcat cggcctggag cattcctttg
    27541 aaagttcctg caaccacgag gttcccttca ttgtcgataa tcatatcgct gatagctgct
    27601 tcgcctgagt taaccagctt acggctccat acaatttctt tgtctggcgt cattttgaga
    27661 atggtagcat cacccaggtc ggtacccgac tgacttggga atgtttgatc aagaatctga
    27721 acactgttgc caccaaaaag ggcagctata taaatgtcac cggcattgtc aaccaccata
    27781 cgtaccggtt gaatctgatt gcctgccaca ttgctacccc agtaggtgta ccattccata
    27841 acttgtgcat tgacattgca taccaatgca agaaatacga ttaaaagaga taaatgtttt
    27901 ttcataaagt tctaattttg aagttagtaa tagtattgtt gatctaataa tgattgcctt
    27961 gtttttccca ttggttccat cttccctgtt ttccgaaaga cagaaccaaa gggtattgtt
    28021 tttaattagt atccatcgtt ctgatgtagg tttttattca ggaacacttc atcatagggt
    28081 agcggtaata aaatttcata agctttaaag ctggtgtact tgttcccaac attgggttct
    28141 tccacacttt gcataaatgc cacaaagtcg ttgtcgttag gatacagtct tctcagatcg
    28201 aaccagcggt ggttttcgaa cgacaactca actcttcttt cgtggaagat tgcatcgcgg
    28261 agtgttgggt atttggcgtg ataagcggca tttgaatcat cgtatttagg caggctggcg
    28321 cgcacacgta ctttgtttaa gtaatttttg gcttcatttg tcatgccttt cttttcgtag
    28381 gcttcagcca gcatcagaaa tacgtcggca aaacgaattt caatccagtt gttaccatgg
    28441 taaccggttg aatttgacaa gtcaacgtat ttttttgtat aatattgatt gtttggtgct
    28501 ttcatgatgg ctatgggagt acggcggtca ccggcttcat attccagaaa caagttttcc
    28561 tgaccggcat tgaagccacg tccattgatt tgtgaagtca aacctttcaa tcccattggc
    28621 tggaagtact gtgcaaacgg cgatgattca cttgaagtac tcagatacat gcattggaaa
    28681 atgatttcct cattgccttc gttagatgtt ccgaaaacac ggggataagt tgctcccgcc
    28741 gggaagctgt cgtatccttt tagcgtaatg gccttctgta ggtaggtaat ggcattggta
    28801 tagtactcat ccttattctg tgtggcagcc atatgaagat agacccttcc aagcaaagct
    28861 gcacctgccg tacttgttgc gcgaccaaag ttatcattgc cggcatcgta gaatgcaggt
    28921 acaacagttg aatttgctaa tgtttccaag tcgctgatga taaggtcgta tgcttcggat
    28981 aatggttgac gggtcatggc gtttgcctcg gtgatggttt cgaccacctt atcgaccacc
    29041 ggaacaggtc caaataagcg tactaagtag aagtgagaca atgcccgtag aaattttgct
    29101 tcggctatta ttctttcacg gctctcatcg ctcattttta ccccgtcaat gtgtttgagt
    29161 accaggttag ccttataaat ggtattgtac aatccgttcc agatccttgc cacgatggta
    29221 ttgttcgagg ttacctgatg ggaaacaaag gtatagtaca atccacctga agttcctgag
    29281 tcaaaagtgg ttgcgttatc cgatttcatt tcggtagcga agaacatcca gtcgtagaaa
    29341 gaccgtgtgg cttgataggc agccaacaag gcatcttcca tgtcctgctc actttgataa
    29401 aacttgtaca catcgaatgc gttcgggttt cttaaattca aaaattccgt atcacaactg
    29461 ttcagtaaac caggaatcaa gacaattaat agatatttaa aatatttttt cataatgtga
    29521 ttttagttat tagaaggata attttacacc tacactatag gaacgagcta tcggataagt
    29581 accaaagtcg atacccggag ccaggtttcc ggagttgtaa tcaacatcag ggttgtaacc
    29641 taagtaatca gtaaacatta aagcgttgtc aaccgatccg aacacatata cattactgat
    29701 gtcaagttta ttgaggaacc ggtttttgga tatgttgtat cctaatgtga tgtttgtaca
    29761 gcgtaggaat gaagcgtctt caacaaaata agttgagaac ttggtgttca ggtttttggc
    29821 tgaacggttg gcgcgatata ttaatccatt tcccggattt gcctcgtcgc ggtagcggtc
    29881 aatacccgat ctcatggtgt ttaccccacc ttcctgattc gctatataat aacgtgagaa
    29941 attgataaca tctgctccgt actgtccgtc gaaagtcgct gaaaagctta gccctttcca
    30001 attaaagctg gtgttgaatc cgaaagtaaa atcgggatag ttgtttccga taatttccct
    30061 gtcgtcttcc gtgattttac cattgccgtc tacatctttc cattttacgt cacctatgta
    30121 cacgttatca taatcagcaa cgggaggtcc taccagttca taacccggtg tgctttggtt
    30181 ggctttatct attaggatat tagcgtattc ttcggcattg atgacgcctt gactaaccat
    30241 gccataaaag gaaccaatcg gtagtccgtt tcgagttata tgcgtgattt ggccgcgctg
    30301 agctttggtt atgatttcat cactgagtcc catcacctta ttacggttga ccgagatatt
    30361 aaatccggca ttccacttca cgttttgggt tgcaatgagg cttccgtcaa tctgtaagtc
    30421 aaatcctctg ttgtacactt taccttcggc aagattggtc caggaacctg tgtaacctgt
    30481 tacagccgaa acgcttgtat tgtataataa gtcgttggta acactatgat aatagtttgc
    30541 aatcaggttc aaacgccctt taaggaaacc taagtctgct ccgatgtttg tctgatttgt
    30601 tttttcccat ccgattttct gatctacgaa ggctgaggga tagtaggtaa ataccgttcc
    30661 ctgtcctaag ttgtaagtcc cctgagccat attgggaatg tgccggtaat tgctgatatt
    30721 gaagttaccg ctgattcccc agcttgctct gagtcgtacg gtcataatat cgactaatga
    30781 agcaaaaaac tcttcattgg acattttcca gccggcagaa acagaaggga aataccccca
    30841 acgtttggcc ggtccgaaac gtgaactgcc atcaccacgc agagacgcgt tgattacata
    30901 gcgactgtcg taactataaa tgacgcgacc gaaaaacgac atgatagacc atgcggcacg
    30961 ggaagttacg gctgaggttc cgttttcgtc cgtagcagtc ggacctgcgg cacttaaatc
    31021 cggaatacgg tcgtttttga atccctgccc gcgagcatcg atattatcgt agtttttctt
    31081 ctgcatcgaa taacccgcca gaccattaac agtatgtttc ccgaattttt tgttgtaatt
    31141 caacgtggtt tcaagtaacc agtcggtatt gaagtcgcgg tcattgctgg cacgggcatg
    31201 caggtcgaaa cttccgggga aaccgtttga ttgccctatt tctcctgcag gacgataata
    31261 gcgataaatt tgatccgaaa tctgtaagcc ggcatttact tttgcataca gaccatcaaa
    31321 gaatttatag cgagcgtctg tattgacact gtatcttgtt cttgtgtaat actgttgtat
    31381 cagatctttc aatgcaaccg ggttctctcc ggcaaaagtc gaatagggaa agttacgata
    31441 agtccaaccg gtagcatagc tgccatcttc ttcatagaca ggatattgag gaggcattcc
    31501 caaggtgttg aggataagac cgtcgttaaa agccaatcca tcggttctga cagtccgctc
    31561 ggtcacatag aacatgtttg aatttactcc cacagataaa cgatcggaca catcaacgtc
    31621 catatttaaa cgggaagtta ttctcttgtg attggagggg gcaacaatac catcctgatc
    31681 caagtaaccc agactaaaca tatacttata tccgctcttt cctccggaga tacttacacc
    31741 cgtgcgatgc atgggtgcct tgctgaacaa agcatcctgc cagtcggtat cgtattttgt
    31801 agcaagaaaa tcgggtgaga gcaacatcag tttggatgct ccgttggcag ttcttgtgtc
    31861 gttgtcgtct tcacggctgt gtgtccatct ttcctcgtcg ctaccgtaaa tattgttgat
    31921 gcgcagaaaa tcgaaataag tgttgttata gccgtcgcgg tgtaattcac gtaactcgtc
    31981 agcgttcatc atgtcgattt ttcttgataa ttcctgtatg gagaattggt agtcgagttc
    32041 aaccctggtt ttatcctttt cacctctttt ggttgttata agtaccacac ctccggctgc
    32101 ccgcgatccg taaatggcag cagaggcagc atctttcaaa acttcgatgg atgccacatc
    32161 ggaggggttg atgtatacgt cttttgacgc aggaaatccg tcaaccacaa ataagggctc
    32221 cacgcttgta taaacggaat tgataccacg aatgcgaata ctggttcctg aaccgggcgc
    32281 tccggatact tgtttgattt gtaccccggg agtatgtccg accaacgatt gttcgagatt
    32341 ggcggtggat ctattcaatt cttcggtttt gatgctggca accgaaccgg ttaagtcact
    32401 tttacgtaca gtaccataac cgataacaac catttcgtcg agcattattg catcttcatt
    32461 caactgtata gtcagatttt gttggttgcc tacaactact tcggaagtgg tataaccaat
    32521 gtaactgacc tgcaaggttg attgatttgt aacctctaac gtaaatttac catccacacc
    32581 ggtaacggtt cccttgtttg tggataaatc aacgacagtt gctccgataa ccggttcatg
    32641 attaacatcg gttaccaaac cggttatgtt tttggtagta atttgtcgag cggtatttcc
    32701 acgagttacg acgatattct tgtctctaat ctcgtatgac acgttttgat ctttaaggat
    32761 ctgttcaatg acttcttcta tcgacttttg atttgcttga acagaaataa gttttccagt
    32821 gtccaaatct ccagcttcga agacaaacgt gtagccgctg ttttgtttta attgctcaat
    32881 agcctctttt actgttatat ttctaacatt cagtgttatg gattgagaga atgccgaaaa
    32941 cgagaaacag caaagaacca agatcattaa aatgaatttc atctttcttt tcataaatgg
    33001 atgctttttt aaaaatatac ccgaaggtat gcttaccaag atatgttttg atgaaatcca
    33061 tactaagatt gtgttacaaa caatgaaaaa tcggctgttt ggtgttgatt tacagagcat
    33121 tacttccctc tggagacttc taatgtgaat gtattgttaa tacggtgctt gcacacagca
    33181 cgaataagat ttgctcgagg tgaggattga aaaaatatgc acaaggcgca atttttgcgt
    33241 tcgatgttgt aatgtgattg tgttcttacc ggacgatggc aaaaggtcaa tgacagaatt
    33301 ttttacattg gtctataaat tgtcaaaaat caagctttgt ctcttgcccc gaatacacca
    33361 gaccggccgt ttttgggact tcggtatcca ggccggcagg acattgggga ctgaccaaaa
    33421 caaattcaaa
//
LOCUS       DAOSTD010000008        33305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1460_length_33305_cov_14.520241, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000008 DAOSTD010000000
VERSION     DAOSTD010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33305
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1460_length_33305_cov_14.520241"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(287..1525)
                     /locus_tag="PLI37_01015"
     CDS             complement(287..1525)
                     /locus_tag="PLI37_01015"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HOQ57631.1"
                     /translation="MKINIFDKKSTMRPWACISIILLLTTAPLGNAQNKSVARVPFNM
                     LGSYIVVKAKINESSGLHFILDTGIRNTILTEWHTNDSLSLVINEERYVQGLGGGDLI
                     FAYVSENNSIQLGKKLTFEKKTIVLLHEDVLELSRRIGSPINGLLGADLLEDYVVQID
                     FVAQRLIFYLKEDFKLPRKYKSLPLTIEQQKLYVPLKVKDNAKEYQLKMLLDTGAGLC
                     AWLRTIGSDVVPIPEKKVYGRIGEGISGEVTGYLARIPTICLGGFCLSNPVVVFPDSA
                     CISDLVIHTGRDGTVGNQLLSRFTLFIDVGGRQLHLHPNSHFKKKFSYNVAGLELIQS
                     IRGLPRYEVAGVWPDSPAAQAGILPGDVIRQVEGDNAYAMSLSELRGYFEQSRKRTLR
                     VVLERDGKLLTLNLDLKSKI"
     assembly_gap    1624..1723
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1771..2580)
                     /locus_tag="PLI37_01020"
     CDS             complement(1771..2580)
                     /locus_tag="PLI37_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOQ57632.1"
                     /translation="MGLCLSACLQIEPRGFFVSYESVDQRFEQSMTWNDQHPFPTLVS
                     SEEEYEFYAVSDIHAGKTNNLGLFLEQALLQQPIAVFMAGDLTTGNANDYHELYAVLP
                     PQDSLPLFPIPGNHDLYFGGWEQYRLLFGSSVYSFSIKTSQTSDLYICLDNAGGTLGA
                     LQLEWLKELLLYERPQHRYCVLVNHNNWVRLRRMLATNPYVEELEVLLELCLKHRVDL
                     VISGHDHKSHTAVFGNTLFITLDALRDSYPTPSYLIVSVGPDAINYSYRNL"
     gene            complement(2598..3323)
                     /locus_tag="PLI37_01025"
     CDS             complement(2598..3323)
                     /locus_tag="PLI37_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57633.1"
                     /translation="MQNTYKALYLILLIMACCYPANSQVELDYLLDLGQTNVSQGLYI
                     STALVSAYSFDKYTLKAGAQFQLIHPSSRVFSAAEFVAGRCFYIGKQTLEAEAFYLYR
                     NFSSLMFISNWGLVVGMRKKHWRFDLGQDFKAYRVREKALSYETEGRVIRENFNLIYR
                     IAYFVNSPEKPWNISLSLTNRDYFLINQETNSMLSLRAETYVSSTMRLYLENWYKPAG
                     ALNMSAHYFGYFARTGFIWTPAF"
     gene            complement(3344..4534)
                     /gene="mnmA"
                     /locus_tag="PLI37_01030"
     CDS             complement(3344..4534)
                     /gene="mnmA"
                     /locus_tag="PLI37_01030"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOQ57634.1"
                     /translation="MKEKIVLGLSGGVDSSVAAFLLKEQGYEVIAVYMINWKDTTGTL
                     SGACPYEEDVLIAELVARKLGIPFHVVDLSDTYRRQVVDYMFEEYARGRTPNPDVLCN
                     REIKFDAFLQTAQKFGAGKIATGHYCRCDSVSIGDGKVYYRLLAGRDKNKDQSYFLCQ
                     LSQKQLSRAVFPIGELDKGRVRELAEELGLASAQKKDSQGICFVGKVDLPVFLQQKLG
                     SKKGRILEVPAYFKAYHRSYPEDPEERIKKQARPYFYRASDGVLVGEHQGAHFYTVGQ
                     RKGLHVGGTEKPLYVLATDVENNLVFVGRGEEHPGLFRKALRINHQEVHWVRPDLALK
                     TGESADYSVRIRYRQALQPAHLHCFEDALYIAFEEEQRSIVAGQFAVWYRGDELLGSG
                     VISA"
     gene            complement(4568..5026)
                     /locus_tag="PLI37_01035"
     CDS             complement(4568..5026)
                     /locus_tag="PLI37_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOQ57635.1"
                     /translation="MEPIVFAYLGIGLMVGLTCVGSAIGVTICGNAVIGALKKNPDQT
                     GAYIGLSALPSTQGLYGFVGYFFMSSYLLPGMNWYAAAAIFGGCLGLGLSGLISAIRQ
                     ADVCANGITAIGSGHNVFGMTMVLAVFPELYAILALLVLILIRGLIPMPV"
     gene            complement(5089..6939)
                     /locus_tag="PLI37_01040"
     CDS             complement(5089..6939)
                     /locus_tag="PLI37_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOQ57636.1"
                     /translation="MKKYSFLVYHAIYIDFLENLREIGVVDVIEKQLGHVEEHSELYE
                     KLEERKRINDLLKLLKQRFPEGLDQAPVDTRIQREDLLRRFESILSEQTQLETKIQVL
                     NREISNLSVWGDFDPQRLEQLREAGCRLDFYFCPRSGFKPDWEEQYRALKIADSGNAV
                     YFITFSFDGQQPQIDAEHLRWPQKSLSELQLEVSALKARRLELEDQLKTEALNQYPSL
                     LYQDKLLSDQFNFSKVLIGTEKAAGDKIMILEGFVPCDREDKLKDLLSREGIYYLAEQ
                     PKQEDKIPVLLKNNRFARLFEPITKMYSLPNYSELDLTPLLAPFFMLFFGLCLGDGGY
                     GLLILLAATLIKPKLGENTRLFATLAQVLGFSTVLVGILTGSFFGVALESLEWSWLKG
                     VKQYFLTENNFGAMFGGYHPLMILSIVIGVVQILYAMCINVVKISIQVGFKYAMSNLA
                     WVVLLIASMVTFGLPALGVTLSVPLTYGLYGLLGLAVLIIVFYNSPGKNVFINVGSAI
                     WDTYNMASGLLGDTLSYVRLFALGLTGSILGSVFNSMAFGMTEAMPGWIRWLAVLLIL
                     LVGHGLNIALNVIGALVHPLRLTFVEFYKNAGFEGGGKAYNPFRKSAQTS"
     gene            complement(6948..7556)
                     /locus_tag="PLI37_01045"
     CDS             complement(6948..7556)
                     /locus_tag="PLI37_01045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOQ57637.1"
                     /translation="MAIQYQYNKTSLQNLEKQLKIRERALPTIKNKESALRLEVKKAK
                     AETRELEKKLEMLIENYREMHALWTEFNAELIKVKDVELGVRKVAGVRIPVLEEVLFE
                     IAPYSMFHAPKWYAGGLDLLKKLATAGIEQDFSVRKLQLLEYARKKTTQKVNLFEKVQ
                     IPGYQDAILKIKRYMEDEENLSKSSQKIVKTRQNAQKQEESL"
     gene            complement(7560..8885)
                     /locus_tag="PLI37_01050"
     CDS             complement(7560..8885)
                     /locus_tag="PLI37_01050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOQ57638.1"
                     /translation="MATKAFQKIYTKITQITKATCSLRAANVGNEELATVDGNLAQVV
                     KIIGDEVTLQVFGGTEGLRTNAEVVFMGKAPSLNVGDQLIGRFFNAFGDPIDGGPVPE
                     GKEVEIGGPSVNPVRRKQPSELIATGIAGIDLNNTLVSGQKIPFFADPDQPYNQVMAN
                     VALRAQTDKIILGGMGLTNDDYLYFKNVFSNAGVLDHIISFVNTTEDPPVERLLIPDM
                     ALTAAEYFAVEKNEKVLVLLTDMTLYADALSIVSNRMDQIPSKDSMPGSLYSDLAKIY
                     EKAVQFPDGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLRRDTDVGKVIVDPFR
                     SLSRLKQLVIGKKTREDHPQLMNAAVRLFADAANAKTKLENGFDLSDYDERCLDFAKE
                     YSAQILAIDVNIDTTEMLDTTWKILAGHFRVEELNIRQDLVNKYMPKVS"
     gene            complement(8897..10654)
                     /locus_tag="PLI37_01055"
     CDS             complement(8897..10654)
                     /locus_tag="PLI37_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOQ57639.1"
                     /translation="MSTKGIVKGIISNLVIVQVDGPVSQNEICYILHKEARLMAEVIK
                     VVGPQAYVQVFESTRGLKVGFEVEFSNHMLEVQLGPGLLSRNLDGLQNDLDKMQGVFL
                     SRGEYTSPLDEKQLWEFQPLAQVGDKVQAADWLGETKENFQAHKIMVPFVLEGSYTVK
                     SIVPQGSYTVKETIAVLLDDSGNEIPVTMVQRWPVKKALKAYKEKPRPFRLLETGVRI
                     IDIANPIVEGGTGFIPGPFGTGKTVLQHAISKQAEADIVIMAACGERANEVVEIFTEF
                     PRLEDPHTGRKLTERTIIVANTSNMPVAAREASVYTAMTIAEYYRSMGLKVLLMADST
                     SRWAQALREMSNRLEELPGQDAFPMDLSAIISSFYGRAGYVILNNGQTGSITFIGTVS
                     PAGGNLKEPVTEGTKKAARCFFALEQIRADRKRYPAINPIDSYSKYIEYPEFEAYIAE
                     NLSDEWIPTVNEMKTLLQRGKEVQEQINILGDDGVPLDYHITYWKSEVIDFVILQQDA
                     FDKVDALCPIERQQYMLKKVMGITRLNFEFDNFTEVMDYFKRVIHVLRQMNYSEFQSE
                     PFRELETELEAVLKERVTQ"
     gene            complement(10672..>11120)
                     /locus_tag="PLI37_01060"
     CDS             complement(10672..>11120)
                     /locus_tag="PLI37_01060"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOQ57640.1"
                     /translation="PNKFVREWFEFELNMNNILTALNCRKYRLPVAGQIIGNNAIAES
                     LKISTVRDFNVSGLFSRMDGLLAIADEENLLEREKMIDRLKWDYLDEHSVHYYFSVEV
                     LLVFLIKLKSLERWLSLDPKSGEESFRSLVNSLRTSARMPADAHIK"
     assembly_gap    11121..11220
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11297..11884)
                     /locus_tag="PLI37_01065"
     CDS             complement(11297..11884)
                     /locus_tag="PLI37_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57641.1"
                     /translation="MNSIKDLTDQLFQEGVEKGRKEAQQLIDEAEKKKSELIAAARQE
                     AAQIIEAAKKQAQELDKNTKAELQLFARQALDALRSEIVNLLTDKVVREAIQTSSNDK
                     TFMQNLLLVFVKNWAENGELEIQTSEAKALTDFFKAKAKELLDKGLTIKQVNNLDTHF
                     ILTAKDGSYKIQFGEEEFINYFKEFLRPQLVEMLF"
     gene            complement(12077..13693)
                     /locus_tag="PLI37_01070"
     CDS             complement(12077..13693)
                     /locus_tag="PLI37_01070"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ57642.1"
                     /translation="MKYITKLVLLLSLGALLTTSCEKMLDTDSERFVPEKDHIIDDEL
                     DAMYAMMGVLTKLQLLGDRYVVLGELRADLMDVTENSESELRALSNLEAGKDNPWLEA
                     DDYYKVINHCNYLINNLDTSLMSGGKNILLPEFAQAKVIRAWTYMQLALNYGKAYYLS
                     TPILTIEEALHSYPVLEKEAILDSLILDLESIQYVQNPDYGDFGSSFYSTYYFLSARF
                     LLADLYLWKNEYAKAASKYFELALDEGRSVTSTYTSTWRSVTPPLVSPNWHAIFDKLR
                     FGPEITAAIGYYNSTSFGDYKESSLALMTQTNYTLAPSANALELFENQVYMYGEEEVA
                     TIIVPGDLRGESGSYEYTERLEDNTIQISDKPRITIYNGEAVYLQRNALLYLRYAEAV
                     NQLNKPSLALAAINYGLSKANIQTYVKPNEIDSGVPWQEFSADVFTSNVGTRSRGQGN
                     KSKVTFPGSLQTLQDSVAFVEEVLVEECALESAFQGNRFHDLMRMSLHNNNYSIIAGA
                     IAKKHKANYAAVYAKMMDPVNWYLPYPDVE"
     gene            complement(13715..16924)
                     /locus_tag="PLI37_01075"
     CDS             complement(13715..16924)
                     /locus_tag="PLI37_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOQ57643.1"
                     /translation="MKNDFKSRVCRLALAVILLLGSTGLAAQQSEIKPVKSQVFIEGK
                     ITDDATGEPINAATIKMGKSSAFTNEEGLFKLQVIGELKPTIIVTAPGYDTREVPHRG
                     QAVMNFTLSSDQFGKALGSKPKKAELDEFINKEGAAVVTMEEIDLTNPMESVESVLQS
                     KFGTIRSVTHSGMPGVGAAMFLRGINTLNVNAKPLFILDGVMLQNFDDQASVHDGFFS
                     NPLTHIDLREVESITVIKDAVSLYGAKAANGVVMIETRRSKDMVTHITANINGGVRSK
                     PQTIPVMDAEQHRLLVSDILKDHPDYDAARLDKMKFFNDNPTFLYYKQNHNNTAWKDF
                     VYRDAVSQSYSLAATGGDEVGMYAIFVNYRNNQGLVRNTSMQQISTRFNSDIVLRDNI
                     SISTKFALSNQSYDLKNDGIISPTSPSGLSYIKAPALYPYSYAKMTGIISTLFEDYDE
                     LGVNNPEAILKKSQQINTQNSFIASAYPTWEINRNLVWKATLGYSMNKLFESYFTPEE
                     GVAPYYLTPSISDYVMKDITVENEARSQTMRSIQTNLDNRLVFTKQFKKHHLQAIGGF
                     RYIGHRMGMDYIKGFNTGEDNLVNISKDLKYRVTDGIDQRTKELSWYLSGSWNYNEKY
                     FAGLAASMDASSRFGYNLKGNALDLFGVSWGLFPSVNASWIASSEDFLAPLKGLDLLK
                     FRLSYGLTGNDDIEDYVTYPYFSSENYLGGGVALVLANLGNPQLKWETTTKANAGVDL
                     SLFNNRFSLSADLFSSNTSDLLVLKQLPSIAGFDYYWSNDGELSNKGYELSANARLLN
                     IGKFRWEVGASLAHYQNELLRLADGDYSTTAYGAEILTAVGQPVGVFYGYKTKGVYGT
                     QAEAEADGLVNRDEVTHLIDYSFGAGDVIFEDFVADGVIDENDKQIIGNPNPDFVGTL
                     SSRIGYKGVNLDILFNYSYGNDVYNFIRRQLESVSDVHNQSTAVLNRWRSENDVTSMP
                     KAVWGDPAGNARFSDRWIEDASYLRLKTLCLSWELPYHIPMVQNLTVWASGNNLWTGT
                     KYLGGDPETSISSRALYQGIDIGLTPQSPSFYIGVKIGL"
     gene            complement(16947..18536)
                     /locus_tag="PLI37_01080"
     CDS             complement(16947..18536)
                     /locus_tag="PLI37_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOQ57644.1"
                     /translation="MKHLTKTVLFTVLALILYSCHQSWEDHYKTQNEALLEKKLMELI
                     NEQSDLSLFASMLKKSGYDKVLSANQAYTVWAPDNAALASVDTSDMDDVNKIVRNHIA
                     RFSYPSSGVQNVAVSMLNGKNVHFIQKEEGLVFGDRKLLKEDLAVGNGILHVIDGMVT
                     YYPNVWEYLSSAANVDSLRDFMYSFEEYFFDERNSLIIGTNDQGQILYDSIIIYSNEL
                     FDYLGNLNHEDSLYTMLFPDNQAWTEAYDTISTYFKYSGSVSTDTVLKAEADLNQSSI
                     TKFVIAKDLIFRGNMTRDELLASDTLVSTGGHVFRDLASLFASATDSTMSNGRCFITP
                     KLNYSTDVWCKPIVVEAEHVRGRIHDRCQLNNRNSLRSWLDTAISNSGYIEVSPISAS
                     AQPVVTFDIPNTLSTAYNIYCVFVPPNVNNKNKDTAELLPTKIRYSIEYLNANGNSTS
                     RTYSRPALYVTNPREMTKMLVTQTAAGVPDPFRFNFCNYEEELTTVRITLTNIVTNAE
                     FESKKFTRDMLIDCIILEPVY"
     gene            complement(18571..20160)
                     /locus_tag="PLI37_01085"
     CDS             complement(18571..20160)
                     /locus_tag="PLI37_01085"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ57645.1"
                     /translation="MKTAFHKIKVAFLAVLLSLGMNSCQDWLTIFPEGELILEDYWKE
                     GAHVEQVVASCYRSLIEPACIERMLVWGEVRSDNMVAGPNVGGDLRDVLDVNILTTNG
                     YANWSSFYTVINFCNTVLLYAPGVVEIDNTYSINQLNAHRAEALAIRALCYFYLVRAF
                     RDVPYITHPSVDGAEDFAMAKTEGHLILDSLILDLKEAKTYAQNNYGVSNVKHNKGRF
                     TRQSIRALMADIYLWQGRYQECVDICREFHRENALLPLASQLRLIDADFMYTEVFNRG
                     NSSESILELQFADDGITNSALNKLYGGGSSGASNSDLSAPSKSLDYSYGSMSAPFKIT
                     DVRWLSFVRGVGSGGYLFINKYVGTQPEQYDNIISYSIRGTTSNFILYRLSDIYLMEA
                     EALNELGEAHLKQALSLVNKTYMRANPSLTDSLSIVDYGNVEAMRELVLLERQREFMF
                     EGKRWFDLVRMVNKGEPIFKLTTIVRRKFNMEAGAEILAVSKMSKMDALYFPILQDEI
                     LSNELLEQNPFYQLEETSAKN"
     gene            complement(20179..23466)
                     /locus_tag="PLI37_01090"
     CDS             complement(20179..23466)
                     /locus_tag="PLI37_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOQ57646.1"
                     /translation="MKTFPKISILLLIAFLLSSTLVYAQGSSEIVRGKVTSSGDNEPL
                     ISVTIMEVDPSNRFISGTVSNYDGEYVLQVKSTQNRLQFKYVGFKTQEIPIGSNRVIN
                     VALDEEALDLAEAVVVGQAVVSDGRFAIPEREISTALQKISTEEFEGIQVSSIDDALQ
                     GRIAGLDIVNVSGEPGSGMSMRIRGTTSISASAEPLVVVNDVPLTYIKIDENFDFATA
                     NEDQYAQLIDVNPDDIESIVILKDAASTAIWGSQGANGVLQITTKRGQRGPTRVSYSY
                     RLSGSRQPKGLKMLNGDDYTMLMKQSYYNPTLENLSAGIPELNYDTNFTEYENFNNNT
                     DWIDAVSRYGFTHDNNLTISGGGEKITFRVSAGYYNQTGTVIGQLLERFSTRSSIEYR
                     VSDRILFTSDFAYTYSNNDRNYENLLGNAYKKMPNVSIYEQDLYGNDTDVYYNIRTDS
                     QLHDSQKNLSNPVAVGMLAKNNTKSYRISPNFSLRYDLLDPYKYNSTLRFNSTVSFSL
                     GNSQKSTFLPSDISNARWNSDKVNLTSNSEDESLDVQFSTSVDYRPAFENRDHSLMLR
                     ANFRVNTGTSGSLGISAYGLPSTIIRDATVPGILGSTSSSTSESRGLSYSWSGHYAYK
                     GRYIFDFTANFNGSSRFGDNFKFGLFPGISGKWIISDEPFWDDYRHIVNMFAFRPSWG
                     IGGNQPSTEYLHLSRYKSYDKGYIDMIAVYPFNIRLTDLRWEKTNSINLGADLEFLKG
                     KYAIDLNYYMKHTTDLLFNKIGLPSHTGYAQLSARNGGTMNNSGWEANLHVRDLVKAG
                     NFRMSFNFNTSNNRNIIVELDDDILATMNPDYTYSNGEYLTRIQERNPYGSIYGFRYK
                     GVYQYNDYIEDVQESAPVVRDKEGNVIFDADGKTKPMYYAYGGSAQYEFKAGDAIYED
                     INHDGTIDELDIVYLGNSLPKFMGGFGSTLRWKQLSLNLFFNFRTGNKVVNAARMEAE
                     NMYTNNNQSIAVNWRWRKEGDVTEMPRALYNYGYNWLGSDRYVEDASFLRFKYMTFNY
                     AVPTQYLKPYKLNTLSFYLTFQNLLVFTNYTGVDPEVGYGSRSVAKDSSKTPRPKDFT
                     LGIKIGF"
     gene            complement(23469..26411)
                     /locus_tag="PLI37_01095"
     CDS             complement(23469..26411)
                     /locus_tag="PLI37_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOQ57647.1"
                     /translation="MFKNTHRAWLFLLSLLLVVGACSDPYPYDDKEPDWLGSSIYDYL
                     VEKGEYTYFTRIIDTCNYREVLAKTGSKTMFVCKDSAFEEYFRNNTDGISRFEDFSVE
                     QLNQIMEYAMVNDASLIETLSYAVGYVKGQVMRRATSLDPFDMLSFEQGDEIPKNKYF
                     EPYREKGLYILNDDTSPTLIHFFEAQMKEKGITDEDFSILFNGKTRQTNDAHLFGIKV
                     IERDITCKNGYIHVLESLMLPRDNMAGYIRKSDSLSIFASLMDRFSAPYYTSSGTELY
                     KTLHPEFRDSIFVRNYFQKVSGTSGAYSILDPHGELIPSDELLRYDPGWNSYQYTSLS
                     MDMGAMFVPDDDAMKFYFSPEGNGSFLHERYKVWDSVPNDIVAMFINAHMKYSFISSV
                     PSKFQGLKDEDGFEIGIEKDNILRARVLCNGAVYVTNEVFPPVDYASVMAPVLVNERT
                     QIFNFALRNLDFNIYLRSLESFYSFLVPFDESFTTYLYPAGQGHETKKMIKLKFNSTS
                     EMVDAVTYTYDEATGQLKDSINVVSTAIALDCLNDIMDYHIVVGDIESGQEYYQTKGK
                     GEIRVQGSGIGMKVWGGGNMEMNRRWGSNWDDSKYTGEVTDILNLKNGKTYFVSNLLQ
                     QPITSVYKTLSNQPEFSKFLSAMMGVPDDMKEHLMLTKDPRTPGLDFNINFFSAFHYT
                     LYAPNNAAMDRAYAAGLPTWDQIAAVPTADDEGAKRAAMIQKVARFLKYHFQDNSVYI
                     GGELLSDVKYETATKNNITNKFHSLRLTQDGSNITIYQSVSNASGIETFNRPPVQVHK
                     ANGLYNLMARDYFFNCATPGDLKSATTTAWTSRTVIHEVDTYFNPIKVPTIRMNNPNY
                     VTTALVPLTADITDNGDGVAYDKNIITKRGFCWSYSREPEWSASGSANQTEVAAASDI
                     VNFKYTLNFPASSNPADTLLYIRAYTESKWAVDGSDGTLEENASKKRLSAYSRELCIN
                     TKKGTIVY"
     gene            26747..28741
                     /gene="dnaG"
                     /locus_tag="PLI37_01100"
     CDS             26747..28741
                     /gene="dnaG"
                     /locus_tag="PLI37_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOQ57648.1"
                     /translation="MIDQITINRILDAARIEEVVGEFVHLRKRGVNYLGLCPFHDDKT
                     PSFSVSPAKGICKCFSCGKGGNVVHFIMEHEQLSYYEALKWLAKKYHIEVVEKQLSPE
                     ELQSKNERESMFVLNEFARDFFKSCLKKVGEGMSIGLKYFLERGLREDTIEKFQLGYA
                     PDERDAFTKNALAKGYQKDFLLKTGLSVADEQGQLFDRYRGRVIFPIHGISGKIRAFG
                     GRILKKDAKAAKYINSPESEIYSKSAELYGIYFARQAIVKQDRCYLVEGYTDVLSLHQ
                     SGVENVVASSGTALTKGQIQLIHRFTDNITVLYDGDAAGIKASLRGIDLLLEEGLNVK
                     VVLLPEGEDPDSFARGHSASALLDYLRKSETDFIRFKTEVLLRDAGDDPVRRAKLIED
                     IVESISLIPNNIIRSEYVKECSRLLDSPELLIQKEVDKKRRKYIERLRQQRGLRESPG
                     TEPAYTPAEPLQQPIPTQADAQKKESTPFSPYDEHERNLMRMIVRYGEQQINLDKNLY
                     PENFRPTVLEFISMDLENEEIEFQHPVYKLMYKEALAHHKEPHFVAERFFLNHHRAEI
                     SLMAGELISEEYELSKIHSRFQVVVEEKDRLHYLVPQFLEELKWKIVKEELAELRSKL
                     KIAEQNKDESTLRDLLEEIKILQELDKELARSLGERIITP"
     gene            28773..29696
                     /locus_tag="PLI37_01105"
     CDS             28773..29696
                     /locus_tag="PLI37_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ57649.1"
                     /translation="MYYVETRSIVKQYTGHLALDKVSIQVPEAKIYGLLGPNGAGKTT
                     LIRILNQITYPDSGEVLLNGNPLKENDVYHIGYLPEERGLYKKMKVAEQALYLARLKG
                     LSKQEAKKRLRYWFDKFNIRDWADKKLEELSKGMQQKVQFIITVLHEPKLLIFDEPFS
                     GFDPVNTQLIKEEILNLKAQGSTILFSTHNMASVEEICDEIGLINKSKLILQGKVQDI
                     KLSHRSGIYLVKLRLPEDPDRAKSQLEKSPFFKIESSSLDPYYWQLELSIRDGLNNNS
                     VFKELYEHFEIVSFEEKIPTMHEIFIKSVKS"
     gene            29693..30982
                     /locus_tag="PLI37_01110"
     CDS             29693..30982
                     /locus_tag="PLI37_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ57650.1"
                     /translation="MNKLSLIIQREYTSRVKKKSFLIMTFVMPLALILIILIPLLLSS
                     VKDNKVRQIAVIDASGLYLPRLQNTPNYQFIPCGDSAEACKNKADYYAYLIIKRNLTE
                     GSPIVRIYSEQQVGADLKDYVQSQLSQMASEDKIASYQIEGLEQIIRDSRVRVKTETI
                     RWTDDGNEQKTSSEISMIIGMITAMLIYFFIFAYGGMVMNGVIEEKSNRIVEIIVSSA
                     KPFQLMMGKIIGIALVGLTQFFLWVVVVLVLVVIGSRFGNIPLDGLSPSMPGTESSLP
                     PGVLDGLQALSSISMGKILFWFVLYFLGGYLLYASLFAAVGGAIDNSEDAQQFTLPMT
                     IPILFALYAGIYGAGNPEGPLTIWCSFIPFTSPIVMMVRLPFGVPFWHLVLSYGLLAL
                     TFVFSTKLAAKIYRTGILMYGKKTTYKVLWQWIKPRR"
     gene            31054..31413
                     /gene="rsfS"
                     /locus_tag="PLI37_01115"
     CDS             31054..31413
                     /gene="rsfS"
                     /locus_tag="PLI37_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOQ57651.1"
                     /translation="MDENQAALKSIIHAIQERKGKKITIVDMSALEHSICRYFVICEG
                     TSNTHILAISDFIREHLYEKMRIKPFAAEGYQNALWIILDYGEIMVHVFHPESRQYYD
                     LEHLWNDAVLTEIPDLN"
     gene            31423..>33305
                     /gene="ftsH"
                     /locus_tag="PLI37_01120"
     CDS             31423..>33305
                     /gene="ftsH"
                     /locus_tag="PLI37_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOQ57652.1"
                     /translation="MSEIQRPKDSKPKPKFNIYWIYALILIGLFAVQFISRKEASKEI
                     TWTEMEEHLVNNRVKEILCINNKNQVRLSIKEEALVSVFGENVTKDKAPVLVVEIPSP
                     DRFSEFIENFNNTHSPDIEVKYDNTSNTFVSVLVSLAPFILLIVIWVLIMRRMAGGGA
                     GGAGGAGGIFSVGKSRARLFEKGGENPITFKDVAGLSEAKQEVEEIVAFLKNPDKYTE
                     LGGKIPKGALLIGPPGTGKTLLAKAVAGEADVPFFSLSGSDFVEMFVGVGASRVRDLF
                     RQAKEKAPCIVFIDEIDAVGRARGKNPNIGANDERENTLNQLLTEMDGFGSNSGVIIL
                     AATNRADILDKALLRAGRFDRQINVELPDINERREIFNVHLRPIKTDDSIDSEFMARQ
                     TPGFSGADIANVCNESALIAARKNKTHVQKQDFLDAVDRIIGGLEKKNKITTAEERRS
                     IAIHEAGHATLSWLLEFAHPLIKVTIVPRGKALGAAWYLPEERQLTTREQMLDEMCST
                     LGGRAAEELFTGKVSSGALNDLERVTKQAYAMVAYFGMSDKLNNLSYFDSSGGSEFSF
                     SKPYSEKTAELIDKEVKALVDEQYLRAKKILSENKDGHQQLANILVEREVIFSEDMEQ
                     IFG"
BASE COUNT         9118 a         7739 c         7325 g         8923 t
ORIGIN      
        1 gcaaaaagct gtttttctca tgatattcct ctttacttac cacggagggt aaaaatgtac
       61 ttttacaaaa ataagctttt ctactgtcag tccaggagct ttttgatatt tttacattta
      121 aattcacaac tgcggccacg caacccggga aattgggtta taatctctga gatcaaaaaa
      181 ttttacagaa taattctgct caagaaacag aacgcattgc tcgataacat gcttctctca
      241 ggaaaatatt gcttaataac agacttccca caatgaagcc tgaactttaa attttcgact
      301 ttaggtccaa attcagcgtg agcagcttac catctcgttc caacacaact cgcagagttc
      361 tttttctact ttgttcaaaa tagcctctca attccgacaa actcatggca taagcattat
      421 cgccttctac ctggcgaata acatcgcccg gcaagatccc tgcttgtgcc gccggggaat
      481 cgggccacac cccggcaacc tcatacctgg gcaaacctct gatgctctga ataagctcca
      541 gtccggccac attgtagctg aatttctttt taaaatggct gttggggtgt aaatgcagtt
      601 gtctgccccc gacatcgata aaaagagtga agcggctcaa caactgattg ccgacggtgc
      661 cgtctctgcc cgtatggata accaaatcgg aaatacaggc cgaatcggga aaaaccacca
      721 ccggatttga aaggcaaaaa ccgcccaggc atatggtggg aattctggcc agatatcccg
      781 ttacttcgcc actgatgcct tcgcctatac gcccgtaaac cttcttttcg ggtataggca
      841 ccacatcgct tccaatggtt ctaagccaag cacaaaggcc agcccccgta tcgagaagca
      901 tttttagttg atattctttg gcattgtctt tgacttttaa cggcacatag agcttctgct
      961 gttctatggt caggggcaga ctcttgtatt ttcggggtaa tttgaaatct tctttgagat
     1021 aaaaaattaa gcgctgtgca acaaaatcaa tctgcaccac gtaatcttcc agtaaatcgg
     1081 cgcccagcaa accattgatt gggctaccga ttcgccggct cagttccaat acgtcttcat
     1141 gcaaaagaac tatcgttttc ttttcgaaag tcaatttttt ccctaattgg atactgttgt
     1201 tttcgctgac gtaggcaaat ataagatcgc cccctcccag tccctgaaca tagcgttctt
     1261 cgttgattac cagcgacaaa ctatcgttgg tatgccactc ggtaaggatg gtgttgcgta
     1321 tgcccgtatc caaaataaaa tgcaggcccg aagattcgtt gatcttggct ttgaccacta
     1381 tataactgcc cagcatatta aatggaactc tggcaacact tttgttttga gcgttcccta
     1441 aaggggccgt tgtcaacagc aggataattg aaatgcaagc ccaaggcctc atggtggatt
     1501 ttttatcgaa gatatttatt ttcattcaaa aaatgaattt catcttcatt tggtgaaaaa
     1561 acctttccgg agacgggcgt atccgcgggg gcctttttca tctcctcttc attctgcgaa
     1621 aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatgaaca atgggggagt
     1741 cccacctatc aacaatgggg gagtcccggc ctataaatta cgataggaat agtttatagc
     1801 atcggggccg acacttacaa tcaaatacga gggagtagga tatgaatctc tcagggcatc
     1861 cagggtgata aagagggtat ttccgaatac ggcagtatga cttttgtgat cgtgtccgct
     1921 gatgaccaga tccaccctgt gtttgaggca taattccaac aaaacctcca attcttccac
     1981 atagggattg gtggccaaca ttctgcgcag cctgacccaa ttgttgtgat taaccagtac
     2041 acaatagcgg tgttgaggac gctcgtaaag cagcaattcc tttaaccatt ccagttgcaa
     2101 ggctcccaaa gttccgcccg cattgtccaa acagatatac aagtcactgg tctgtgaagt
     2161 tttaatactg aaactgtaaa ccgacgatcc gaataaaagt cgatattgtt cccagccccc
     2221 gaaatagagg tcatgattgc ccggtatcgg gaaaagcggc aatgaatcct gaggcggcaa
     2281 aacggcatac agttcatgat aatcgttggc atttccggta gtcaagtccc ctgccataaa
     2341 aaccgctatg ggttgctgca acaaagcttg ctctaaaaac aagccgagat tgttggtttt
     2401 acctgcgtga atatcggaaa cggcataaaa ctcgtattct tcttcggagc tgaccaatgt
     2461 cggaaaagga tgctgatcat tccaagtcat agattgctca aaacgctgat ctacagattc
     2521 atacgaaaca aaaaaacctc tgggttcaat ctgtagacaa gcagaaagac acaggcccat
     2581 acagcacaag cttaatctta gaatgcagga gtccatataa atcctgttct agcaaaataa
     2641 ccaaaataat gggcactcat attaagtgct cctgccggtt tataccaatt ttcgagataa
     2701 agtctcatgg tagaactgac gtatgtctca gccctaaggc tcaacatgga gttggtttcc
     2761 tgatttatca gaaaataatc ccggttggtc aggctcaaac taatgttcca gggcttttcg
     2821 ggactgttta cgaaataggc aatgcggtaa atcaaattga agttttcgcg aatcacacga
     2881 ccctccgttt cgtaagacaa agccttctcc cggacacgat acgccttaaa atcttggccc
     2941 aagtcaaatc gccaatgttt cttcctcata ccgacaacca ggccccagtt ggaaatgaac
     3001 atcagactcg aaaaatttcg atacaaataa aaggcctccg cttcgagggt ttgtttcccg
     3061 atataaaagc atcggccggc aacaaattcg gctgccgaaa atacccggct ggagggatgg
     3121 atcagttgaa actgtgcacc cgccttcaga gtatatttat caaagctgta ggcgcttact
     3181 aaggccgttg aaatgtataa gccctgcgac acattggtct gccccaaatc aagcaaataa
     3241 tccagttcaa cctgagaatt ggcaggataa caacaagcca ttattaaaag aataaggtaa
     3301 agggctttgt aggtgttttg cattggtata ttctcaaaaa atattaagca gaaatcaccc
     3361 ccgaacccag caattcatcg ccccggtacc aaacggcaaa ctgtccggcc acgatgctgc
     3421 gctgctcttc ctcgaaagca atgtacaagg catcttcgaa acaatgcaaa tgggccggct
     3481 gtaaagcctg acgatagcgg atacgcaccg aataatcggc agactctccc gttttcaatg
     3541 ccaggtcggg ccggacccaa tgcacttcct gatgattaat tctaagtgcc tttcggaaca
     3601 aacccggatg ctcttctccc ctgcccacaa agaccaggtt gttttctacg tcggtggcca
     3661 aaacataaag cggtttttcg gttccgccta catgcaagcc ttttcgctgt cctacggtat
     3721 aaaaatgagc cccctgatgt tcgcctacca atacgccatc ggaagcacga taaaaatacg
     3781 gacgggcttg ttttttgatt ctttcttccg ggtcctcggg ataagagcga tggtaggctt
     3841 taaaataggc aggcacctcg agtatccgtc cctttttact gccgagtttt tgttgtagaa
     3901 aaacgggcaa atccactttg ccgacaaagc aaatgccctg cgagtctttt ttttgtgccg
     3961 aagccaaacc cagttcctcg gccagctcac gcactctgcc tttgtcgagt tccccgatgg
     4021 ggaaaacggc tcgtgagagt tgcttctggc tcaattgaca taaaaagtaa gattggtctt
     4081 tgtttttgtc tcgaccggcc aacaggcgat aatacacttt gccgtcgccg atgctgacac
     4141 tgtcgcagcg gcaataatgc ccggtagcaa tcttgccggc accaaacttc tgtgcagttt
     4201 gcaaaaaagc atcaaacttg atttccctgt tgcaaagcac gtcgggattg ggcgtcctgc
     4261 cccgtgcgta ttcctcgaac atatagtcga ccacctgacg gcgataagtg tcgctcagat
     4321 caaccacatg gaagggtatc cccaatttgc gggctaccag ttcggctatc aacacatctt
     4381 cttcgtaggg acaggctccc gacaaggtgc cggtagtgtc cttccaatta atcatgtaaa
     4441 cggcaataac ctcatagccc tgctctttga gcaagaaagc ggcgacgctc gagtcgacgc
     4501 cgccggacaa gccaaggaca attttttctt tcaatgccga tgtgttttat ccgctttcaa
     4561 agcctgatca aaccggcata ggaatcagac cacggataag aatcaatacc aaaagcgcca
     4621 ggatggcgta aagttccgga aaaacggcca agaccatggt cataccaaac acattatgac
     4681 ccgaacctat ggctgtgata ccattggcac aaacatcggc ctgacgtata gccgaaatca
     4741 aaccggacaa tcccaatccc agacaaccgc caaagatagc tgcagcagcg taccagttca
     4801 ttcccggcaa caaataggaa ctcataaaga aatagccgac aaaaccgtaa agcccctgag
     4861 tggaaggcaa agcagacaaa ccgatataag ctcctgtttg gtcggggttc ttttttaagg
     4921 ctccgatgac agcgttaccg catatggtta caccaatggc acttcccaca caagtcaacc
     4981 cgaccatcaa tccaattccg agataagcaa atacaatagg ttccatagtt tgagttttaa
     5041 atttagacga taatattcat agttttaata attccattga gagtgatttc aactggtttg
     5101 ggccgatttt ctaaagggat tgtaggcttt gcccccaccc tcgaaaccgg cattcttata
     5161 aaattccaca aaagtgagac gcaagggatg caccaaagct cctatgacgt tcaaggctat
     5221 gttcaaacca tgccccacca acaaaataag cagtacagcc aaccagcgta tccagccggg
     5281 catggcttcg gtcattccaa aggccataga attgaataca gatcccagaa tactgcccgt
     5341 caggcccaaa gcaaataaac gcacataaga aagtgtgtca cccagcaaac cggaggccat
     5401 attgtatgta tcccatatcg cggacccgac gttgatgaaa acatttttgc cgggagaatt
     5461 gtaaaacaca attatcagga cagccaggcc cagaagaccg tataaaccgt aagtaagagg
     5521 caccgacaac gtcactccta aagcaggcaa accaaaagtg accatagagg caataagcag
     5581 caccacccaa gccagattag acatagcgta tttaaatccc acttgtatgc ttatcttgac
     5641 cacattgatg cacatggcgt acaaaatttg tacaacccct atcacaatcg acaaaatcat
     5701 cagcggatgg tagccgccaa acatggcccc gaaattattt tcggtgagaa aatactgttt
     5761 tactccctta agccatgacc attcgagcga ttccagagct actccgaaaa aagacccggt
     5821 cagtatgcca accagcacgg tcgaaaatcc caggacctgc gccaaagtgg caaataacct
     5881 ggtgttttcg cccaattttg gtttgatcag agttgcggcc agcagaatca acaagccata
     5941 gcctccgtct cccaggcaaa gtccgaaaaa caacataaaa aagggagcca gcaatggggt
     6001 tagatccaat tccgaataat tgggcaggga atacattttg gttatgggct caaaaagccg
     6061 ggcaaaacgg ttgtttttga gcaatacagg tattttatcc tcttgtttcg gttgttcggc
     6121 caggtaatag atgccttcgc ggctgagtaa gtctttgagt ttgtcttccc tgtcgcaggg
     6181 cacaaaacct tcgagtatca ttattttgtc gcccgccgct ttttctgtcc caatcaagac
     6241 tttggaaaaa ttaaactgat cgctgagcaa tttatcctga tacaaaaggc ttgggtattg
     6301 gttcagggct tcggttttga gttgatcttc caactccaaa cggcgagcct ttaaagcgga
     6361 tacttccaat tggagttcgg aaagcgactt ttgcggccag cggagatgtt ctgcatcgat
     6421 ttggggttgt tgtccgtcga agctgaacgt gataaaataa acggcattcc cgctgtcggc
     6481 tattttcaga gcgcgatatt gctcctccca gtcgggtttg aaaccgcttc tgggacagaa
     6541 ataaaaatcc agacgacaac cggcttcccg gagttgttcc agacgttgag gatcgaaatc
     6601 accccaaacc gacaggttgc tgatttcacg gtttaaaacc tgtattttgg tttccagctg
     6661 agtctgctca cttaatatac tttcaaaacg acgaagcaag tcttcacgct ggatccttgt
     6721 gtcgacagga gcctgatcaa gtccttcggg aaaacgctgt ttgagcaatt tcagcaagtc
     6781 gttgatgcgc ttacgttctt cgagcttttc gtacaactcg ctgtgctcct ccacatgacc
     6841 caattgcttt tcgatcacat cgaccacgcc gatttccctg aggttttcga gaaaatctat
     6901 ataaatggca tgatagacca aaaagctgta tttcttcatc ggaacaatca tagactttcc
     6961 tcctgctttt gggcattttg ccgtgttttt actatttttt gcgaagactt ggataaattt
     7021 tcttcgtctt ccatatagcg tttaatcttt aatatggcgt cctggtaacc gggaatctga
     7081 actttttcga ataaattcac tttttgagtg gttttttttc gggcgtattc caacaattgc
     7141 agcttccgga cgctaaaatc ctgttctata ccggctgtgg ccaatttttt gagtaaatcc
     7201 aatcctccgg cataccattt gggagcatga aacatactgt agggggcgat ttcaaacagc
     7261 acctcttcca gtaccggaat tcgtacgccg gctacttttc gcacacccaa ttcaacatct
     7321 tttaccttga taagctcagc gttaaattca gtccacaggg cgtgcatctc cctgtagttt
     7381 tcaatgagca tttcgagttt tttctccaac tccctggttt cggctttggc cttcttcact
     7441 tcaagacgca gggcgctttc cttgtttttg atggtaggaa gagctctctc acgaatcttg
     7501 agttgctttt caagattctg caaggacgtc ttgttgtatt gatattgaat agccatactt
     7561 tacgaaactt tgggcatata cttgttgacc aggtcttgtc tgatgttgag ttcttccacc
     7621 ctgaaatggc cggccagaat tttccaggtg gtatcgagca tttcggtggt atcgatgttg
     7681 acgtcgatgg ctagaatttg ggctgaatat tctttggcaa aatccaggca gcgctcgtcg
     7741 taatcgctca ggtcgaaacc gttttccagt ttggttttgg cattggcggc atcggcaaac
     7801 aaacgcacag cggcattcat cagctgagga tggtcttcgc gggtcttctt gccgatgacc
     7861 agttgtttga gccgagacaa actgcggaag ggatctacaa taactttgcc cacatcggta
     7921 tcgcgacgca aaaacaactg tccttcggtg atataaccgg tattatcggg aatggcgtgc
     7981 gtaatatctc ctcccgaaag ggtggttacc gcaataatgg taatggaacc tccgtcgggg
     8041 aattgtaccg ctttttcgta aatcttggcc aggtcggaat acagagaacc tggcatggaa
     8101 tctttggaag gaatctggtc catacggttg gatacgatcg aaagcgcatc ggcatacagg
     8161 gtcatatccg tgagcaacac caatactttt tcgttcttct ccacggcaaa atattcggcc
     8221 gccgtcaaag ccatatcggg tatcagcaaa cgttccaccg gaggatcttc ggtggtattg
     8281 acaaaactga tgatgtggtc gagcaccccg gcgttactga agacattttt gaaatacaga
     8341 taatcgtcgt tggtcagccc catgccgccc aaaatgatct tgtctgtctg ggctctgagg
     8401 gctacattgg ccattacctg attataaggc tggtcggggt cggcgaaaaa gggaatcttt
     8461 tgtccggaaa ccagggtgtt gttcagatct atgcccgcaa taccggtagc aatcaactcg
     8521 gagggttgtt tgcgccgtac cggattgacc gaagggccgc caatttcgac ttctttgcct
     8581 tcgggcaccg gccctccgtc tataggatcg ccaaaggcat tgaaaaagcg accgataagc
     8641 tgatcgccga cattaaggct gggtgccttg cccataaaga ccacttcggc gttggtgcgc
     8701 aaaccttcgg tacctccgaa aacctgcaag gtgacttcgt ctccgataat ctttaccact
     8761 tgagccagat taccgtcgac ggtagccaat tcttcgtttc cgacattggc agctctcagg
     8821 ctgcaggtgg ctttggtgat ttgggtgatt ttggtgtata tcttttgaaa tgcttttgtt
     8881 gccattatcc cggattttat tgtgttactc gttcttttaa tactgcttcc aattcggttt
     8941 ccaactctct aaacggctct gattgaaact cggaatagtt catctggcga agtacgtgga
     9001 taactctctt aaaataatcc atcacctcgg taaaattatc aaattcgaaa ttcaaacggg
     9061 ttatgcccat caccttcttg agcatatatt gctgacgctc aatgggacaa agagcatcga
     9121 ccttgtcgaa agcgtcctgt tgcaaaatga caaaatcgat cacttccgat ttccagtagg
     9181 taatatgata atcgaggggc accccgtcgt ctcccaggat attgatttgt tcctgcacct
     9241 ccttgcctct ttgcagcaag gttttcattt cgttgaccgt ggggatccat tcgtccgaca
     9301 aattttcggc aatgtaggct tcaaattccg ggtattcaat gtacttggaa tacgaatcaa
     9361 tgggattgat ggccggataa cgtttacggt cggcccggat ttgttccagc gcaaagaaac
     9421 agcgtgcggc ttttttggtt ccttcggtta ccggttcctt caggttaccg cctgcgggcg
     9481 aaaccgtacc gataaaggtg atggatcccg tctgtccgtt attgaggata acataacctg
     9541 cacgcccgta aaagcttgaa ataatggcgg aaaggtccat agggaaagcg tcctgaccgg
     9601 gcaattcttc caggcggttg gacatttccc ggagtgcctg cgcccagcgc gaggtggagt
     9661 cggccatcaa caacactttc aatcccatag agcgataata ctcggcaata gtcatagcgg
     9721 tatacaccga agcctcgcgg gcggccacag gcatgttgga agtattggcc acgatgatgg
     9781 ttctttccgt cagtttgcgt ccggtgtggg gatcttccag cctgggaaat tcggtaaaga
     9841 tttccactac ttcgttggct ctttccccgc aagcggccat aatcacaatg tcggcctccg
     9901 cctgtttgga aatagcgtgt tgcagcaccg ttttcccggt tccgaaaggt ccgggaatga
     9961 agccggttcc gccctcgact atgggattgg cgatgtcgat gatacgcaca ccggtttcga
    10021 gcaggcggaa aggcctgggt ttttctttgt aagctttgag tgcttttttt accggccagc
    10081 gttgtaccat ggtgacgggt atttcattgc cgctgtcgtc gagcaaaacc gcgatggtct
    10141 ctttgacagt ataactgcct tggggtacta tgcttttgac ggtataacta ccctcgagca
    10201 cgaaaggaac cattatttta tgcgcctgaa aattttcttt ggtttcgccc aaccaatcgg
    10261 cagcctgaac cttgtctccg acttgggcca aaggttgaaa ctcccataat tgcttctcat
    10321 ccaaaggaga agtatattcc cctctgctca gaaaaacacc ctgcattttg tcgaggtcgt
    10381 tttgcagtcc gtccaaatta cgggacaata agcccggacc caactgaact tcgagcatgt
    10441 ggttgctgaa ttcaacctcg aatcccactt tcaggccgcg ggtgctttcg aatacctgaa
    10501 cataagcttg cgggccgacc accttgatga cctcggccat cagcctggcc tctttgtgta
    10561 gaatataaca aatttcgttt tggctcaccg ggccatctac ctgcactata accaggttag
    10621 aaatgatccc cttgactatt ccttttgttg acatatcagt gttttagtaa tttactttat
    10681 gtgtgcatct gccggcatac gtgcgctggt tctcagtgag tttaccaggg aacggaaact
    10741 ttcctccccg cttttgggat cgagcgacaa ccagcgttcc aggcttttca acttgattaa
    10801 aaaaaccagc aaaacctcga ccgagaaata ataatggaca ctgtgttcat ccaggtaatc
    10861 ccattttaaa cggtctatca tcttttctct ctccaagagg ttttcttcgt cggcaatagc
    10921 gagcaaaccg tccattcggg aaaaaagccc ggaaacattg aaatcgcgca cggtagatat
    10981 cttcaatgat tcggctatgg cgttgtttcc gattatttgt ccggcaacgg gcaaacgata
    11041 cttgcgacaa ttcaatgccg taagaatatt gttcatattc aattcaaact cgaaccattc
    11101 cctgacaaac ttattgggag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 tacgtcagct gcagtttatt gtcatccaag gaaatgtcgg gaaaacctgc caccaggtaa
    11281 tggtattgac tcataactaa aacaacattt ccaccagttg tggacgtaaa aattccttga
    11341 aataattgat aaattcttcc tctccgaatt gaattttgta agaaccgtct ttggcggtca
    11401 atataaagtg ggtatccaga ttgtttactt gttttattgt caaaccttta tcgagaagct
    11461 cttttgcctt tgccttgaaa aagtcggtca aagccttggc ttcgcttgtt tgaatttcga
    11521 gttctccgtt ttcggcccag tttttaacaa aaaccaataa taagttttgc ataaacgttt
    11581 tatcgttgct gcttgtttga atggcctctc tcactacttt gtcggtcaat aagttgacaa
    11641 tttccgacct taaagcatcc agggcctgac gggcgaacaa ttgcaattcg gccttggtat
    11701 ttttatccaa ttcctgtgcc tgttttttgg cagcttcgat aatttgagca gcctcttgcc
    11761 gggcagctgc aataagttcg gatttctttt tttcggcctc gtcaataagc tgctgggctt
    11821 ccttccggcc tttttccaca ccttcctgga acaactgatc ggttaagtct tttatcgaat
    11881 tcataagatt gttgaaatat gatttagagc cacaatatta ctaatttatt tactgcaccg
    11941 caatgcaatt aagtaaaaaa cattctatgt attggcaaat tataacataa aaaacctccc
    12001 ggcctttggt ccgggaggtt tttttacttc tgtcgccaaa accgacagga attaaaatag
    12061 ggtagaaata taaaatttat tctacatccg gatagggcaa ataccaattg accggatcca
    12121 tcattttggc ataaacggca gcgtaattgg ctttatgttt tttggcgatg gctccggcaa
    12181 taatggaata gttgttgtta tgcagtgaca tcctcatcaa atcgtggaag cggttgccct
    12241 gaaaggccga ttccaaagcg cattcttcga caagcacttc ttcgacaaaa gcgaccgaat
    12301 cttgaagggt ttgcaaactg cccgggaaag tcactttcga cttattaccc tgccctctgc
    12361 tgcgggttcc gacattcgaa gtaaaaacat ccgcactgaa ttcctgccag ggcacgccgg
    12421 aatctatctc gttaggcttt acgtaggttt gtatattggc tttgcttaaa ccataattaa
    12481 tcgctgccaa agccagactg ggtttattca actgatttac tgcttcggca taacgtaaat
    12541 acaataaagc attacgctgc agataaacag cttctccgtt gtatatggta attctgggct
    12601 tatcggaaat ctgaatggta ttatcctcta agcgttcggt gtattcgtaa gatccgcttt
    12661 cgcctctcag gtcaccggga acaataatgg tggctacttc ttcctcgccg tacatatata
    12721 cctggttttc gaatagttcc aaagcattgg ccgaaggagc aagggtataa ttggtttgtg
    12781 tcatcaaagc cagagacgat tctttgtaat ctccgaacga agtggaatta taataaccga
    12841 tggcagcagt aatctccggc ccaaaacgta atttatcaaa aatagcatgc caattgggcg
    12901 acaccagggg tggcgtaacg gaacgccaag ttgatgtata cgtggaggtt acacttctgc
    12961 cttcgtccag ggctaattca aaatattttg aagcagcctt tgcgtattca tttttccata
    13021 agtagagatc tgccaacagg aatctggccg aaaggaaata ataggtactg tagaagctgc
    13081 tcccaaaatc accgtaatcc gggttttgta catattgaat gctttccaga tcaagaataa
    13141 gagaatccag aatagcttcc ttttccaaga caggataaga atgcagggct tcttcaatgg
    13201 taagaatagg cgtacttaag taataagctt tgccgtaatt cagggccagt tgcatatagg
    13261 tccaggcccg aatcacttta gcctgggcaa attcaggtaa taatatattt ttccctcccg
    13321 acatcaaaga agtatccaaa ttgttaatca aataattgca atgattgatc accttgtaat
    13381 aatcgtcggc ttcgagccag ggattgtctt taccggcttc cagattgctc aaagccctga
    13441 gttcagactc tgaattttcg gtcacatcca taagatctgc cctgagttcg cccaagacga
    13501 cataacgatc gcctaataat tgtagtttag tgagcactcc catcatggca tacatggcat
    13561 ccagttcgtc gtcgataata tggtcctttt cgggaacaaa acgttctgag tccgtatcca
    13621 acattttttc gcaggaagta gtcagcaagg cgcctaaact cagtaataaa accaattttg
    13681 ttatgtattt cattgtactg tcttattagt atgattacaa acctattttt actcctatat
    13741 aaaaggaggg actctgagga gttaaaccta tgtctatccc ctgataaagg gctcgactgg
    13801 aaatcgaagt ttccggatca ccccccaggt atttggttcc cgtccacaaa ttattgcccg
    13861 aagcccatac cgtaaggttt tgaaccatag gaatgtggta gggcaactcc cacgacaaac
    13921 acagcgtttt taacctcaag tacgatgcat cttcgatcca acgatccgaa aagcgggcat
    13981 ttcctgccgg atcgccccaa acagccttag gcatactggt gacatcgttt tccgaacgcc
    14041 aacgattcaa tacggcagtg gattgattgt ggacatcgct caccgattcg agttgcctgc
    14101 ggatgaaatt gtacacatcg ttaccgtagg aatagttgaa caagatatcg agattgactc
    14161 ctttgtagcc tatacggctg ctcaaagtac ccacaaaatc ggggttgggg tttcctataa
    14221 tctgcttgtc gttttcatca ataactccat cggcaacaaa atcttcaaag ataacatcac
    14281 cggcgccaaa actataatca atcaaatgag tcacttcgtc tctgttgacc aaaccatcgg
    14341 cttcggcctc tgcctgagtg ccgtagacgc ctttggtttt gtagccgtag aaaactccga
    14401 caggttggcc tacggctgtt aatatttcag cgccgtaagc cgtggtggaa tagtctccgt
    14461 cggccagcct aagcaattca ttctgataat gtgccagcga agcccctact tcccagcgga
    14521 atttaccgat attcagcaaa cgggcgttgg ctgagagttc ataacctttg ttggacagtt
    14581 cgccgtcgtt gctccagtaa taatcgaaac cggctatgga aggcaattgt ttcagtacca
    14641 acaaatcgga agtattcgag ctaaacagat cggcagacaa agaaaagcgg ttgttaaata
    14701 aggaaaggtc cacgccggca ttagctttgg ttgttgtttc ccatttaagt tgaggattgc
    14761 cgaggttggc caaaaccagg gctactccgc cacccaagta attctcagac gagaaataag
    14821 gataggtgac atagtcctca atatcgtcgt tacccgtcaa accgtaactc aacctgaatt
    14881 tgagtaaatc cagaccttta agaggagcaa ggaaatcttc cgaagatgcg atccaggaag
    14941 catttaccga ggggaaaagg ccccagctga caccaaacaa atcgagggca ttgcccttga
    15001 ggttgtagcc aaagcgcgag gaagcatcca tagaggcagc cagaccggca aaatattttt
    15061 cattgtaatt ccaacttccg ctcaaatacc aggacaactc tttggtccgt tgatctatac
    15121 catcggttac ccgatacttc aggtccttac tgatatttac caggttgtct tctccggtat
    15181 taaaaccttt gatataatcc attcccatcc tgtggcctat ataacgaaag cctcctatag
    15241 cctgcaagtg atgcttttta aattgtttgg taaaaacaag gcggttgtcg agattggttt
    15301 gaatgctgcg catagtctgg cttcgggcct cattctctac ggtgatatcc ttcatcacat
    15361 aatcggagat actgggagtc aggtaatagg gcgctacgcc ttcttcgggt gtaaaataag
    15421 attcaaacaa tttattcata ctgtatccca aagtagcctt ccaaaccaga ttcctattaa
    15481 tttcccatgt cggataagcc gatgcgataa aactgttttg agtattgatt tgctgcgatt
    15541 tcttcagtat ggcctcggga ttattcacgc ccaattcgtc gtaatcctcg aacaaagtag
    15601 aaataattcc cgtcattttt gcgtaggaat agggatacaa ggcaggagcc ttgatgtaac
    15661 tcagtcccga aggagaagtc ggagatatta ttccatcgtt cttcaggtcg taactttggt
    15721 tactcagggc aaatttggta gaaatgctta tgttgtctct caatacgata tccgaattaa
    15781 agcgggtact gatttgctgc atactggtat tacgcaccaa tccctggttg tttctgtagt
    15841 tcacaaaaat ggcgtacatt cccacttcgt cacccccggt ggcagccaga gaataactct
    15901 gacttacggc gtcgcggtaa acaaaatcct tccaggcggt gttattgtga ttctgtttgt
    15961 agtaaaggaa agtgggatta tcgttgaaaa acttcatttt gtccaatctt gccgcatcgt
    16021 agtcgggatg atcctttaaa atatcgctga ccaaaagacg atgctgctcg gcatccataa
    16081 caggaatcgt ttgcggtttg gagcgcactc ccccatttat attggctgtg atatgagtta
    16141 ccatatcttt gcttcgcctg gtttctatca tcactacgcc gttggcagcc ttggccccgt
    16201 acagcgagac ggcatcttta attacggtaa tgctttctac ttctctaaga tctatatgtg
    16261 ttaagggatt ggagaaaaaa ccatcgtgta ccgaggcctg atcgtcgaaa ttttgcaaca
    16321 ttaccccgtc caagataaac aagggtttag cattcacatt cagcgtattg attcctctga
    16381 gaaacatagc ggcacctacg ccgggcatgc ctgaatgtgt aaccgaacgt atggttccaa
    16441 atttcgattg cagaacactt tcgacggatt ccatggggtt ggtcaggtca atttcttcca
    16501 tggtaacaac cgccgctcct tctttattga tgaactcgtc caattcagcc tttttaggtt
    16561 tactgcccag ggcttttcca aattgatcgc tcgacaaagt gaagttcata acagcctgac
    16621 ctctgtgagg aacctcacgg gtatcgtaac ccggagcagt aacaataata gtgggtttaa
    16681 gttctcctat cacctgaagt ttaaaaaggc cttcctcgtt ggtaaatgcg gaagattttc
    16741 ccattttgat cgtagccgca tttataggct caccggtagc atcgtctgtg atcttgccct
    16801 ctataaaaac ttgcgacttg accggtttaa tttcgctttg ttgcgcagca agacccgtac
    16861 ttcccaataa aagaattacc gccaaggcta aacgacaaac acgagattta aaatcatttt
    16921 tcatagtgta aagcttcttt tatagattag taaacgggtt caagtataat acagtctatc
    16981 agcatatcgc gggtgaactt cttggattca aattccgcat tggtaacaat gttagtcaga
    17041 gtaatccgca ccgtagtaag ttcttcctcg taattacaaa agttgaagcg aaaaggatcg
    17101 ggtaccccgg cggcggtctg cgtgacaagc atcttggtca tttcacgtgg attggtcacg
    17161 tacagtgcgg gacgactgta agttctggaa gtggaattac cgttggcatt caaatactca
    17221 atactataac gtattttggt cggcaataat tctgccgtat ctttattctt gttgttcacg
    17281 ttgggcggca caaacacaca ataaatattg taagcggtac tcagggtgtt gggaatatca
    17341 aaagtaacaa ccggctgagc actggcagag atgggactta cttcaatata acccgaattg
    17401 gatatggcgg tatcgagcca tgaacgcaaa gaattacggt tatttaattg gcaacgatcg
    17461 tgtatacgtc cgcgaacatg ttcggcctcc accactatgg gtttacacca aacatcggtg
    17521 gaataattca gttttggagt gatgaaacat cttccgttgc tcatagtgga atcggtcgca
    17581 gaagcaaaaa gtgaggcaag gtccctgaaa acatgtccgc cggtagaaac cagagtgtca
    17641 cttgccaata gttcatctct tgtcatgttt cccctaaaaa tcaaatcttt tgctataacg
    17701 aatttggtta ttgagctttg atttaaatcg gcctcggcct ttaaaacagt gtctgttgaa
    17761 acagaaccgg aatacttgaa ataggtactg atcgtatcgt aagcttcggt ccaggcctga
    17821 ttgtcgggaa agagcatggt gtacaggctg tcttcgtgat tcagatttcc cagataatcg
    17881 aatagttcat tggaataaat aatgatagag tcgtaaagaa tctgtccctg atcgtttgtt
    17941 ccgataatta aactgtttct ttcgtcaaaa aagtattctt cgaaagaata catgaaatcc
    18001 cgcagggaat cgacattggc ggcgctgctc aggtattccc aaacgttagg ataataggtc
    18061 accattccat cgataacgtg caaaataccg ttgcccaccg cgagatcctc tttgagtagt
    18121 ttcctgtcgc caaaaacaag cccctcctct ttttgtataa aatgaacgtt ctttccgttc
    18181 aacatactga ctgccacatt ttgcactccg gaactggggt aggaaaaacg agcaatatga
    18241 ttgcgaacaa ttttgttgac atcgtccata tcgctggtat caacgcttgc caaagcggca
    18301 ttatcggggg cccataccgt atacgcctga ttggccgaca acactttgtc gtagcctgat
    18361 tttttgagca tagaggcaaa aaggctcaaa tcggactgtt cgttaatgag ctccatcaat
    18421 ttcttttcta ataaagcctc gttttgcgtc ttgtaatgat cttcccagga ctgatggcaa
    18481 gaataaagta tcaaggccaa gactgtaaac aaaacggttt tagttaaatg cttcatattg
    18541 agtatattta ttaatcgtat tggttaagca ttaattcttg gcagaggtct cttccaattg
    18601 atagaaaggg ttttgctcca ataattcgtt acttaaaatt tcatcctgca aaatcggaaa
    18661 atacaaggca tccattttgg acatcttact tacggccaaa atttcagctc ctgcttccat
    18721 attgaattta cggcgtacta tggtggtcag tttgaaaatc ggttctcctt tgttgaccat
    18781 tctgaccaag tcaaaccaac gctttccctc gaacatgaat tcccgttgac gttccagcaa
    18841 caccagttcg cgcatggctt ccacattgcc gtaatcgact atactcagcg aatcggtcag
    18901 agagggattg gcgcgcatat aggttttgtt aaccagcgaa agggcctgtt tcaaatgggc
    18961 ttcacccaac tcattcagcg cctcggcctc cataagataa atatccgata agcgatagag
    19021 tataaaatta ctggtagtgc ccctaataga ataagaaata atgttgtcgt attgttcggg
    19081 ttgagttccc acgtacttat taatgaagag atagccgcct gaacccacac cccggacaaa
    19141 actcagccat cgtacatctg ttatcttaaa gggagccgac atactgccgt aggaataatc
    19201 caaagatttt gagggagccg acaagtcgct gttggaggca ccacttgaac ctcctccgta
    19261 taatttattc aaagcggagt ttgttattcc gtcgtccgca aattgcaatt ccaatatact
    19321 ttcggaggaa tttccccgat tgaagacctc ggtatacatg aaatcggcat ctatcaacct
    19381 caactggctg gcaaggggta agagggcatt ctcgcggtga aattcccggc aaatatccac
    19441 acattcctga taacgtccct gccacagata gatatcggcc atcagggccc tgatggattg
    19501 cctggtaaaa cgccccttat tgtgtttgac gttgctcaca ccataattgt tttgagcata
    19561 ggttttggct tctttcaaat cgagaatcag tgaatcgaga ataagatgcc cttcggtttt
    19621 ggccatggca aagtcttcgg cgccgtcaac gctgggatgc gtaatatagg gcacatccct
    19681 gaaagcccta accagataaa agtagcacag ggcacgtatg gccagcgctt ctgctctgtg
    19741 agcattcaac tggttaatag aatatgtgtt gtcaatttca accactccgg gagcatatag
    19801 taacaccgta ttgcaaaaat taatgaccgt ataaaagctg gaccaattag cataaccgtt
    19861 ggtggtcaga atattgacat ccaaaacatc tctcagatca cccccaacat tcgggccggc
    19921 caccatatta tcggaacgga cttcgcccca gaccaacatg cgttctatac aggccggctc
    19981 tatcaaagag cggtaacagg acgccaccac ctgctccaca tgagcgcctt ctttccaata
    20041 atcttcgagt atcagctctc cttcgggaaa aatggtcagc caatcctgac aggaattcat
    20101 acccaggctt aacaaaacag ccaggaatgc cacttttatt ttatgaaatg ctgttttcat
    20161 tgttcgattt aattaagctt agaaacctat ttttataccc aaagtaaagt ccttggggcg
    20221 aggtgtttta ctgctgtcct tggccaccga tctggagccg taacccactt cggggtcaac
    20281 accggtgtag ttggtaaaaa caagtagatt ctggaaggtc aggtaaaaac tcaaggtatt
    20341 caatttatac ggtttgaggt attgagtagg taccgcgtaa ttgaaagtca tgtatttgaa
    20401 gcgtaaaaac gacgcgtctt ccacatagcg gtcactgccc agccagttat atccgtagtt
    20461 atacagagca cggggcatct ccgtaacgtc accttccttg cgccaacgcc agttgaccgc
    20521 aatgctttga ttgttgttgg tgtacatatt ttctgcttcc atacgggcgg cgtttaccac
    20581 tttattgcct gtacggaaat taaagaaaag attgagggac aattgtttcc aacgcaaagt
    20641 actaccgaaa cctcccataa atttgggcaa ggagttgccc aggtaaacga tatccaattc
    20701 atctatagtg ccgtcgtgat tgatgtcttc gtaaatggca tcgcccgctt taaattcgta
    20761 ctgagccgaa ccgccgtaag cataatacat tggcttggtt ttgccgtcgg catcaaaaat
    20821 aacattgcct tctttatcgc ggacaacagg ggcactctcc tgaacatctt ctatgtaatc
    20881 gttgtactga taaacccctt tgtagcgaaa accgtatatg gaaccgtagg gatttctttc
    20941 ctgaatacgt gtcaggtatt ctccgttgga gtaggtataa tcgggattca tggtagccag
    21001 gatgtcgtcg tccaattcaa cgatgatatt ccggttgttg gatgtgttga aattgaagga
    21061 cattctgaaa ttccccgctt taaccaggtc gcgcacatgt aaattggctt cccagccgct
    21121 gttgttcata gttcctccgt tgcgggcact taattgggca taacctgtat gactgggtaa
    21181 gccgattttg ttaaacaaca aatcggtggt atgcttcatg taatagttca ggtcgatggc
    21241 atatttcccc ttcaaaaatt ccaggtcggc acctaagttg attgaatttg ttttttccca
    21301 cctcaggtcg gtgagtctga tattgaaagg atatacggca atcatgtcga tataaccctt
    21361 atcgtagctc ttgtaccgac tcaagtgcag gtattcggta gagggctggt tacccccgat
    21421 accccaactg gggcgaaaag caaacatatt gacgatgtgt ctgtaatcgt cccaaaaagg
    21481 ctcgtccgat ataatccact tgcccgaaat gccgggaaac aaaccaaatt taaagttatc
    21541 accaaaacgg gagcttccgt tgaagttggc cgtaaaatca aaaatatagc gccccttgta
    21601 cgcatagtgt ccgctccaac tgtaactcaa tccgcggctt tcactggttg aactgctggt
    21661 tgagcccaaa ataccgggca ccgttgcatc gcggatgatg gtagaaggaa gaccatacgc
    21721 agaaataccc agagatcccg aagttcccgt attaacacgg aaattggcgc gcagcatcag
    21781 gctgtggtcg cgattctcaa aagccggccg atagtcaaca ctggtagaga attgaacatc
    21841 gaggctttcg tcttcggaat tactggtcag gtttacctta tcgctattcc aacgggcatt
    21901 ggaaatatcg ctgggtagaa aagtactttt ttggctgttc cccaaactaa aggacacggt
    21961 tgaattaaat ctcaacgttg aattgtactt atagggatcc agcaaatcgt aacgcagtga
    22021 aaaattcggc gaaatacgat aacttttggt gttgtttttg gctaacatac ccaccgctac
    22081 cggattgctc agatttttct gactatcgtg tagttgagag tctgtacgaa tgttgtaata
    22141 cacgtccgtg tcgttgccgt aaaggtcttg ctcgtaaata ctgacgttgg gcattttttt
    22201 gtaggcattg cccaaaaggt tttcgtagtt cctgtcgttg tttgaatacg tataggcaaa
    22261 gtccgaagta aataaaatac ggtcggaaac acggtattct atgctggagc gggtggaaaa
    22321 acgttccagc aactgaccga taaccgttcc tgtctgattg taataaccgg ccgatacgcg
    22381 gaaagtgatt ttttcgcccc ctcccgaaat agtcaggtta ttgtcgtgcg taaaaccata
    22441 gcggctgact gcatctatcc agtcggtgtt gttgttgaaa ttttcgtatt cggtaaaatt
    22501 ggtgtcgtaa tttaattcgg gtatgccggc agaaaggttt tccaaggtgg gattgtaata
    22561 cgattgcttc atgagcatcg tatagtcatc tccgttcaac atttttaaac ccttaggctg
    22621 acgggaaccg gaaagccggt aggaataaga aacacgcgtt gggccgcgtt gacctctttt
    22681 ggttgtaatc tgcaaaacac cgttggctcc ctgcgatccc cagatggccg tcgaagccgc
    22741 gtccttcaga atcacaatgg attcgatatc gtccgggttg acgtcgatca actgggcgta
    22801 ctggtcttcg ttggctgtgg caaagtcgaa attttcgtca atttttatgt aagtaagcgg
    22861 aacatcgttg accaccacca aaggttcggc actggcactg atggatgttg taccccggat
    22921 acgcatggac atcccggatc cgggttcgcc cgatacgttc acaatatcca gaccggcaat
    22981 acggccttga agggcatcgt cgatagacga cacctgtatg ccttcgaatt cttcggtgct
    23041 gattttttgc agagcggtcg aaatttcgcg ctcgggaatg gcaaaacggc cgtcactcac
    23101 cacggcctgt ccaactacca cagcttcggc caggtccaag gcctcttcat caagagccac
    23161 attgataacc ctattgcttc caatgggaat ttcctgagtt ttaaagccga cgtatttaaa
    23221 ctgtagtctg ttttgtgtac tttttacctg caacacgtat tctccgtcgt aattacttac
    23281 ggtaccgctg ataaaacgat tggacggatc cacttccatg atggtaacgg aaatcaaagg
    23341 ttcattatcg ccggaactcg tcacttttcc tcgtactatt tcggaactgc cctgtgcata
    23401 aaccaaagta ctgctcaaca aaaaggcaat gagtaacagt atgctgattt tgggaaatgt
    23461 cttcatgatt aataaactat tgttcctttt ttggtgttga tacataactc tctactatag
    23521 gcactcaggc gttttttgga tgcattttcc tctaaagttc cgtcactgcc gtctactgcc
    23581 catttgcttt cggtgtaggc acggatgtat aataatgtgt cggcagggtt ggacgatgcg
    23641 gggaaattca aggtgtattt gaaattaaca atgtccgaag cggcagctac ctcagtctgg
    23701 ttagccgaac ctgaagcgga ccattcgggt tctctggaat aactccagca aaatcctctt
    23761 ttggtgatga tattcttatc gtaagccact ccgtctccgt tatcggtaat atcggccgtc
    23821 aaaggcacca gtgccgtggt aacgtaattg ggattgttca tgcgaatggt aggtaccttg
    23881 atcggattga aataggtatc cacctcgtga ataaccgtcc tcgatgtcca ggcagtggta
    23941 gtagccgatt taaggtcacc gggggtagcg cagttaaaga aataatccct ggccatcaga
    24001 ttatacagtc cattggcttt gtgtacctgt acgggaggcc tgttgaaagt ctcaataccg
    24061 gaagcattgg aaacagactg ataaatagtt atgttggaac catcctgagt taagcgtaaa
    24121 gaatggaact tattggtaat attatttttg gtcgccgtct cgtactttac gtcactcagc
    24181 aattctccac cgatgtaaac cgagttgtcc tggaaatgat acttcaggaa acgagccact
    24241 ttctgtatca tggcagccct tttggcgccc tcgtcgtcgg ctgtaggtac ggcggcaatt
    24301 tgatcccagg tgggtaaacc ggccgcatag gccctgtcca tagccgcatt attaggcgca
    24361 taaagggtgt agtgaaaggc cgagaaaaag ttgatattga aatccagccc cggggttctg
    24421 ggatctttgg tcagcatcaa atgctctttc atgtcgtcgg gaactcccat catggccgac
    24481 agaaatttgg aaaattcggg ttggttgctc aatgttttat agaccgaagt gataggctgt
    24541 tgcaataaat tggagacaaa gtaggtttta ccgttcttca aattcaggat atcggtcact
    24601 tcgcctgtgt atttgctgtc gtcccagtta ctgccccaac gccggttcat ctccatatta
    24661 ccgccgcccc atactttcat acctataccg ctgccctgaa cacggatttc tccttttcct
    24721 ttggtttgat aatattcctg tcccgactca atgtctccca ccacaatgtg gtaatccata
    24781 atatcgttca ggcagtcgag ggcaatcgcg gtggatacaa cgtttatgga atcttttagt
    24841 tgacctgtag cttcgtcgta ggtgtatgtt acagcatcaa ccatttcgga agtagaattg
    24901 aatttgagtt tgatcatctt cttggtttcg tgtccctgtc cggcaggata gagataggtg
    24961 gtaaaggatt cgtcgaaggg aaccaggaag ctgtaaaagc tttccagaga tctcaggtat
    25021 atattgaaat ccaagtttct gagagcaaag ttaaatattt gagtccgctc attgaccaaa
    25081 acgggagcca ttacagaagc ataatctacc gggggaaaga cttcgttggt aacatatacg
    25141 gctccattgc ataaaaccct ggcacgaagg atattgtctt tttctatgcc aatctcaaaa
    25201 ccgtcttcgt ccttgagacc ctgaaacttg ctgggcaccg aactgataaa ggaatacttc
    25261 atgtgggcat tgatgaacat ggcgactatg tcgttgggaa cactgtccca aaccttataa
    25321 cgttcatgca aaaaagagcc gtttccttcg ggagaaaaat aaaatttcat cgcatcgtcg
    25381 tcgggcacaa acatcgcacc catatccatg gacaatgaag tgtattgata agaattccag
    25441 cccgggtcgt agcggagcaa ttcatcggaa ggaatcaatt cgccgtgggg atctaaaatg
    25501 gaatacgcgc ccgatgtacc gcttaccttt tggaaataat tacgcacaaa gatggaatcc
    25561 ctgaattccg gatgcagggt tttgtataat tcggtaccgg aggaagtata ataaggtgcg
    25621 ctgaagcgat ccatcagaga agcgaagata gacaggctgt cggatttccg gatgtaaccg
    25681 gccatgttgt cgcgcggcaa cattaggctt tccagcacat ggatgtaacc gtttttacaa
    25741 gtgatgtccc tttcgatcac cttaataccg aataagtgag catcgttggt ctgacgtgtt
    25801 ttaccgttga agagaatgct aaaatcttcg tcggtaatgc ccttttcttt catctgagcc
    25861 tcaaaaaaat gaattaaggt gggcgaggtg tcatcgttca aaatatataa acccttttca
    25921 cgataaggct caaaatactt gttcttcgga atttcatctc cctgctcaaa agaaagcata
    25981 tcaaaggggt cgagcgaagt ggcacggcgc atcacctggc ctttaacgta acctacggca
    26041 taagacaagg tttcgatcaa tgaggcatcg tttaccatag cgtattccat tatctgattc
    26101 agttgctcta cactgaaatc ttcgaaacga cttatgccgt ccgtgttgtt tctgaaatac
    26161 tcctcaaaag cggaatcctt gcagacaaac atcgtcttgc ttcctgtttt ggccaatact
    26221 tcacggtaat tacaagtatc gatgatcctt gtgaaatagg tgtattcgcc cttttctact
    26281 aaataatcgt agatgctgga accgagccag tcgggttctt tgtcgtcgta gggataagga
    26341 tcggaacaag ctccaacgac caagagcaat gaaagtagaa acaaccaggc tctgtgcgtg
    26401 tttttaaaca tactctatga attaaaaggc gttattttag ttaattttgg acgtaattat
    26461 tgagagttct gtatataagg tactattgag gaagtaaatt tagcgctttg cggcaaattt
    26521 aaggtatatc ttgaggaaaa atccatactg aatttatcaa tattggcatt tatgtattaa
    26581 ttttgcccgc aaagataaat gaaatactta atacacctaa ttcgatttag cgatattttt
    26641 gataatttta gttatcgccc tgaacgccta tgtaatttga ctttcgttaa acctaacagt
    26701 cgtattttta actaagtttt cggagcaccc cggcacacct tcgagcatga ttgatcaaat
    26761 caccataaac agaatattag acgcagcccg tatagaggag gtagtaggag aatttgttca
    26821 tttacgcaaa agaggagtaa attatcttgg cctctgccct tttcacgacg acaaaacgcc
    26881 ctcattcagt gtttctccgg ccaaaggcat ttgcaaatgc tttagttgcg gcaagggagg
    26941 taatgtggtt catttcatta tggaacacga acaactcagt tattatgagg cacttaagtg
    27001 gctggccaaa aaataccata tcgaagttgt agaaaaacaa cttagccccg aagaactgca
    27061 aagcaaaaac gagcgggaaa gcatgtttgt actcaacgaa tttgcccggg atttctttaa
    27121 atcctgttta aagaaagtag gcgaaggcat gagtataggt cttaaatatt ttttagaaag
    27181 aggtctgcgc gaagatacca tagaaaaatt ccagttgggc tacgcccccg atgaacgcga
    27241 cgctttcaca aaaaacgccc tggcaaaagg ctaccaaaaa gactttctgc tcaaaaccgg
    27301 cctgtctgta gccgacgagc agggacagtt gtttgaccgt taccggggca gggtgatttt
    27361 tcccatacac ggcatttcgg gcaaaataag ggccttcgga gggcgtattc ttaaaaagga
    27421 cgccaaagca gctaaataca tcaattctcc cgaatcggag atatacagca aaagtgccga
    27481 attgtacggg atttattttg ccaggcaagc catcgtcaaa caagatcgtt gttacctggt
    27541 ggaaggttat accgatgtgc tttccctgca ccagtccggt gtcgaaaacg tggtggcttc
    27601 atcgggcaca gccctgacca aaggacaaat tcaacttatc caccgtttta ccgacaacat
    27661 caccgtgctt tacgacggcg acgctgccgg cattaaagct tcgctgagag gcatcgacct
    27721 gctgcttgaa gaaggcctca acgtcaaagt ggttttgctg cccgagggtg aagacccaga
    27781 ttcttttgcc cggggacaca gtgcctcggc attgctcgat tacctcagga aaagcgaaac
    27841 cgactttatt cgcttcaaaa ccgaagtgct cctgcgcgac gccggcgacg acccggtgag
    27901 aagggcaaag ctcattgaag atattgtcga aagcatctcc ctgattccca acaatatcat
    27961 tagatccgaa tacgtcaaag aatgcagccg actgctcgat tctcccgaat tgctgattca
    28021 aaaagaagtc gacaaaaaac gccggaaata catcgaacgc cttcgtcaac agagagggtt
    28081 gagagaaagc cccggaactg agcccgctta tacgcctgcc gagccgctgc aacagccaat
    28141 ccccacgcaa gcggatgctc aaaaaaaaga aagtacacca ttcagccctt acgatgaaca
    28201 cgaacgcaat ttgatgcgca tgatagtccg ctacggcgaa cagcaaataa acctcgacaa
    28261 aaacctctac cccgaaaact ttcggcccac ggtgctcgaa ttcatttcca tggatctcga
    28321 aaacgaagag atcgaatttc aacatccggt ttacaaattg atgtacaaag aagccctggc
    28381 tcaccacaaa gaacctcatt ttgtagccga acgctttttt ctaaaccatc accgggccga
    28441 gatcagcctg atggcgggag aattgatctc tgaagaatat gaactgagca aaattcacag
    28501 ccggttccaa gtggtcgtag aagaaaaaga cagattgcat tacctggttc cccaatttct
    28561 cgaggaactg aaatggaaga ttgtcaaaga agaactggcc gaactcagat ctaaacttaa
    28621 aatagcggaa caaaacaaag atgaatcgac cctgcgcgac ttgctcgaag aaataaagat
    28681 actccaggag ttggacaaag agctggccag aagcttgggg gaaagaataa ttacaccctg
    28741 attttacaca ttcaaattaa ctacagcact gtatgtatta tgttgaaacc cgaagcattg
    28801 ttaagcaata caccggacat ttggctttgg acaaagtgag tatacaggtg cccgaggcta
    28861 aaatctacgg cctgttaggc cccaacggcg ccggaaaaac cacccttatc cgcattctta
    28921 accagattac ttatcccgac agcggagaag ttttgctgaa cggaaaccct ctaaaagaaa
    28981 acgatgttta ccacattggc tacctacccg aagaaagggg cttgtacaaa aaaatgaaag
    29041 tagccgagca agctctttac ctggccagac tcaaaggctt gtcaaaacag gaggctaaaa
    29101 aaagactcag gtactggttc gacaaattca atatccgaga ctgggccgat aaaaagctcg
    29161 aggagctttc caaaggcatg cagcaaaaag ttcaattcat catcaccgtt ctgcacgaac
    29221 ccaaactcct gattttcgat gagcccttca gcggcttcga tccggtgaat acacagctga
    29281 ttaaagaaga aattttaaac ttaaaagccc agggatccac catacttttt tcgacgcaca
    29341 acatggcctc ggtcgaagaa atctgcgacg aaatcggttt gatcaacaaa tccaaactaa
    29401 tactacaagg caaagtgcag gacatcaagc ttagccaccg cagcggcatt tatctggtaa
    29461 agctcaggct acccgaagac cccgacaggg ccaaaagcca actggagaaa tctcctttct
    29521 tcaaaatcga aagcagctcg ctcgacccat actactggca actggaattg agtatcagag
    29581 acggattaaa taacaacagt gttttcaaag aactctacga gcacttcgag attgtttctt
    29641 ttgaggaaaa aattcccacc atgcacgaga tttttattaa aagcgtaaaa tcatgaacaa
    29701 actttcgctg attatccaac gggaatacac ctcccgggta aagaaaaaat cttttctgat
    29761 aatgaccttt gtcatgcccc tggccctgat attgatcata ctcataccgc ttttattgtc
    29821 ttcggtcaaa gacaataaag tcagacaaat cgcggtcatt gacgcctccg ggctctatct
    29881 tccccgcttg cagaatacgc cgaattatca atttattcct tgcggcgaca gcgccgaagc
    29941 ctgcaaaaac aaagccgatt attacgccta ccttattata aaaaggaatt tgacggaagg
    30001 cagccctata gttagaattt attctgaaca acaagtggga gccgacctca aagattatgt
    30061 acaaagccag ctcagtcaaa tggcctccga agacaaaatt gcttcctacc aaattgaggg
    30121 ccttgagcag ataatccgcg attcgagagt cagggtgaaa acagaaacca tacgttggac
    30181 cgacgacggc aacgaacaaa aaacctcttc ggaaatctcc atgatcatcg gtatgattac
    30241 tgccatgctc atctacttct ttatttttgc ctacggagga atggtaatga acggtgtgat
    30301 tgaagaaaaa tccaaccgca ttgtggagat cattgtttca tcggccaaac cctttcagct
    30361 tatgatgggg aaaataatag gtattgccct ggtaggcctt actcaatttt tcctttgggt
    30421 agtggtggtg ttggttttgg tggtaatcgg cagccggttc ggcaatattc cgctcgacgg
    30481 actaagccct tcgatgcctg ggacggagag ttcgcttcca cccggagtcc tcgacggttt
    30541 gcaggctctc agttctattt ctatgggaaa aattctgttc tggtttgtgc tttacttttt
    30601 gggaggttac ctgctgtatg cctctttgtt tgcagccgtc ggaggagcca tcgacaacag
    30661 cgaggatgct caacagttta cccttcccat gaccattcct atacttttcg ccctgtacgc
    30721 aggcatctac ggcgccggca atcccgaagg gccgcttacc atctggtgtt cgttcattcc
    30781 atttacttct cccattgtaa tgatggtccg tttacccttt ggtgtgcctt tttggcactt
    30841 agttctgtca tatgggttgt tggcactgac atttgtattc agcaccaaac tggctgcgaa
    30901 aatctaccgc acaggtatcc tgatgtacgg taagaagacc acttacaaag tattatggca
    30961 atggatcaag cctcgtcgct aaattaattg gtacaatgtt tgcggtacaa tgcaaatgaa
    31021 agcgtatctt tgtcgctgtt caaaaacgat tctatggatg aaaaccaagc tgcattaaaa
    31081 agtattatcc acgccattca ggaacgaaaa gggaaaaaaa tcactatagt agacatgtcg
    31141 gccctggagc attctatctg caggtatttt gtcatttgcg aaggtacatc caatacacat
    31201 attctggcca tctccgactt tatacgggag catttatatg aaaaaatgag aatcaaaccc
    31261 tttgctgcgg aaggttacca aaacgcccta tggatcattc tcgattacgg cgaaattatg
    31321 gttcatgttt ttcatcccga aagcaggcaa tactacgatt tggaacatct ctggaacgat
    31381 gccgttttga cggagattcc cgatcttaat taagtaaact ttatgagcga aatacaacgt
    31441 cccaaagaca gtaaacccaa acccaaattc aatatctact ggatctacgc tctgattctg
    31501 ataggattgt ttgctgttca gttcatttca agaaaggaag ccagcaaaga aattacctgg
    31561 acggaaatgg aagagcatct ggtaaacaac agagttaaag aaattctctg tatcaacaac
    31621 aaaaaccagg ttcgcttaag tataaaagaa gaggctctgg taagcgtatt tggcgaaaat
    31681 gtgactaaag acaaagcgcc ggttttggtc gtggaaattc cttcgcccga tcgtttttcc
    31741 gagtttatag aaaatttcaa taatactcat tcgccggata tagaggttaa atacgacaat
    31801 acttccaata cctttgtgtc cgtcctggtc agcctggctc cttttatttt gctcattgtc
    31861 atttgggttc ttataatgag gagaatggcc ggcggaggcg caggcggtgc aggcggcgcc
    31921 gggggcattt tcagtgtagg aaaatccaga gccaggttgt ttgaaaaagg cggagaaaac
    31981 cccattacct tcaaagacgt tgccggtctg agcgaagcca agcaggaagt agaagaaatc
    32041 gtagccttcc tcaagaatcc ggataagtac accgagcttg gaggtaaaat acccaagggt
    32101 gccctgctga taggccctcc cggaaccgga aaaacgctcc tggccaaggc cgtagccggc
    32161 gaagccgatg ttcctttctt ttcactttcc ggttcggatt tcgttgaaat gtttgtagga
    32221 gtgggtgctt cgagggttcg cgatctcttt aggcaagcca aagaaaaggc tccctgcatc
    32281 gtttttatcg acgaaattga cgctgtcgga agggctcgcg gcaaaaatcc caacataggt
    32341 gccaacgacg aacgcgaaaa caccctgaac caattgctca ccgaaatgga tggtttcggt
    32401 tccaacagcg gtgtcatcat cttggccgcc accaaccggg cagacatttt agacaaggcc
    32461 ttgctcagag ccggccgttt cgatcgccaa atcaatgttg aattgcccga cattaacgag
    32521 cgccgcgaaa tttttaatgt acacctgcgc cccatcaaaa cagacgacag catcgattcg
    32581 gaatttatgg caagacagac accaggcttt tcgggtgccg acatcgccaa cgtctgtaac
    32641 gaatctgccc tgatcgctgc ccgcaaaaac aaaacccatg tgcaaaaaca ggattttctc
    32701 gacgctgtgg accgtattat aggcggcctg gaaaaaaaga acaaaataac caccgccgag
    32761 gaacgccgtt ccattgccat acacgaagcc ggtcatgcca cactgagctg gctgctcgaa
    32821 tttgcccatc cgctcatcaa agtgaccatt gtacccaggg gtaaggctct gggtgcggcc
    32881 tggtatctgc ccgaagagcg acaattgacc accagggaac aaatgctcga tgaaatgtgt
    32941 tccaccctgg gtggcagggc tgccgaagaa ttgtttaccg gcaaggtctc ttccggtgca
    33001 ctcaacgacc tggaaagggt aaccaagcaa gcttacgcca tggtcgccta ttttggtatg
    33061 agcgataaat tgaacaatct ctcctacttc gactccagtg gcggttccga attcagtttt
    33121 tccaagcctt atagtgagaa aaccgccgaa ctcatagaca aggaggtaaa agctttggta
    33181 gacgaacaat acctgagagc aaaaaaaata ctgagcgaaa acaaagatgg gcatcaacag
    33241 ctcgccaata tcctggtcga acgcgaagtt atcttctccg aagatatgga acagattttt
    33301 ggcaa
//
LOCUS       DAOSTD010000009        33080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1468_length_33080_cov_12.766752, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000009 DAOSTD010000000
VERSION     DAOSTD010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33080
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1468_length_33080_cov_12.766752"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="PLI37_01125"
     CDS             <1..1218
                     /locus_tag="PLI37_01125"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOQ57653.1"
                     /translation="TVNNFFTMYEWRCYSSEDMVNWTDHGAVASLKNFKWMDRTNGAW
                     APHCIERNGKFYLYVPIHGEGIAVLVSDSPTGPFTDPIGKRLVESDHIWQDIDPMVAI
                     YDGQAYLYWGNPNLWYVKLNKDMVSYDLGIGDKGIVSVDMTPEAFGSQKGPGDRVRTT
                     YTEGPWLYKRNKTYYMVYASCGIPERISYSTSTSPEGPWTYRGVIMDRADHLAFTIHP
                     AIIDFKGNSYFFYHDQVLSGGQGFKRSVSVEQFDFNSDGSIPLIPSTREGVKQALSVL
                     KPFKKVEAETMAWSRGLKTGSDSLTGVYVTDINNGDFQLIRNVDFGRGAKVFQASLAS
                     DSDGGLIEIRLGHPEGELIGTLEVLNTGGRQQWKTLSCELKKINGVHDLCFVFKGGEG
                     ALFNFDYWMFKRN"
     gene            1685..4681
                     /locus_tag="PLI37_01130"
     CDS             1685..4681
                     /locus_tag="PLI37_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ57654.1"
                     /translation="MRKLILLLVSGFALSFSLLAQVRIEGRITDQSGEALPGVNVVED
                     GTNNFAVSDVNGNYVLTDVNRNSTLIFSFVGMLREEIQVGNRTRIDVVMREDAVSLEE
                     VVTIGYGTVKKRDLTGAVQSIKSAEITQIPTGNMMQALQGRVAGLDIVRRSGRATSGV
                     EMVLRGKRSISGSNTPLFIIDGVIGNYEDINPNDVESIEIMKDASSTAIYGSAGANGV
                     IIVTTKKGQKGKAVVNVDSYYGVNGFLQFPPVRTGEDYIELRRQANLTTGAWARGDAD
                     SKLFSDPEWDAIQKNQWVDWFELGTRDGVLQNHSVSVSGGSENTIGYFSLNYYNEEGI
                     LKSDDISKYSFRANVEHQAKKWLKAGLNINGAFTDQNERKGQYFTRVLCLLPLGTPYN
                     EDGTINPFPLAGDTQLSPIADMAEDQYVNNRIALGVNPTSYLQINPIKGLTAKSVFSA
                     YLNFSRQGLFKGALSADGYGEGKSSAQIINGNRYNYKWENIVTYDFSIDDVHNFGITG
                     VTSWSENQGENASILGYNLNWNKYLFYNLGATDMQSRVATSSYVRTQMMSYAARVNYN
                     YMGRYFLTVSNRWDGASILAEGNKWDYFPAAALAWRISDEGFMQGLDKLDNLKLRLGY
                     GVTGNAGASAYATLNFGVAGSNLAFQETPAPYYMFSQNLANQVLDWEKSYNWNLGIDL
                     NMFKNRLNITTELYNTDTKDILFQRNLPASTGGYKAGNYSIWENVCETQNRGIEFEVN
                     TVNIKSNNFSWSTTLSLAANREKITKFVSEDPVTNGSTYLVEGYPIQSFYDYKYLGIW
                     QEDEATEAALYGRVPGDVKIEEVLVDSTYTTADRQVIGSPTPKLIFGLSNTFTYKGID
                     LTVLLDARIGQTMSYGILGWYNPNGEGNGPAVVDYWTPENPEGRFPRPNASYSRFANL
                     PLGTNSLFYIDGSYCKVRNVTLGYSLPNNLLRKLNLNKARIYATASNPVIFTKSKYLK
                     NYDPELGGADEFPLAKQLVFGINLSF"
     gene            4703..6454
                     /locus_tag="PLI37_01135"
     CDS             4703..6454
                     /locus_tag="PLI37_01135"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ57655.1"
                     /translation="MKTINKLQILTAVVLGLLLFNACSLEEYNPSGGPTTDEYFSTPT
                     GYEQLINACYFPLTRSWTGGGEDYVVFMAECGTDLWTCPQGEGWMKEVFYYTGLNGGT
                     AHLNEGWQSSYESINYCNAAIRFAPKAGYTDDAVRDAKVAEAHFLRAFFNFFIVEQFG
                     GVYLPKVETTKPITDIPRSSVSEFYELIFSDLKFAMTHLPKIQTERGRATRAAAYHLY
                     AKACLQYASYDAAIDKRTLYTEAKDAALEIINNKASYGLALYAEPADIFNVANNKDNT
                     EAVWVATHSKSSTLNPRGSKYWNRVFKQFGCLQENGICGVFHDINSEYVKFDKRIMPS
                     LKLLDLYGPKDMRYQAFFREEYYATADYTWSEGHCSKFGKDIATFAGIKTIAADSLAM
                     LFTRADIADPLALNYACFDRDLIYNADGSANINYTNYGYPALKKYEAPGMYAGELHKA
                     YSWSDNLTYRLAETYLLAGEALYRLDDPLAVNYFNEIRNRACLNHDGSMNIAMSDIGV
                     DFILAERARELCGEYTRFMDLKRMGKAIFSSYILSNPDISKRQAFDADIHFVRPVPEK
                     MELNYQLNPDDFQNPGY"
     gene            complement(6550..7137)
                     /locus_tag="PLI37_01140"
     CDS             complement(6550..7137)
                     /locus_tag="PLI37_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOQ57656.1"
                     /translation="MALLKRLQQLPVFHNARLVGGTALALHLGHRNSIDLDFFGSFEY
                     SLIDFTDAFNREGFTFNSTQNTSLINIFDIEGVKVDIVNYPFEWLEAPVEDDGIILAG
                     MKDIASMKLAAITNRGTKKDFIDIYFLLQHYSLNEMLQFYKLKYKTDAIYNVIRSLVY
                     FADAESDPLPKMYVPLQWNDVKSSIKDSVKEISIF"
     gene            complement(7125..7442)
                     /locus_tag="PLI37_01145"
     CDS             complement(7125..7442)
                     /locus_tag="PLI37_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57657.1"
                     /translation="MEKEEDKKNPITEFSPNLFWDVTVEDLDMEQHKAFIINRVLDYG
                     QWEDWKIILNHYGLNQIASIARNLRSLFPKSLAFISTVSHIPEDQFRCYKHLQSKNKL
                     WHF"
     gene            7679..9196
                     /locus_tag="PLI37_01150"
     CDS             7679..9196
                     /locus_tag="PLI37_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HOQ57658.1"
                     /translation="MIIGKRLNLEEFRAIVEGKAKIDVDDEVKKSVVECFDFLEDFSK
                     DKVIYGINTGFGPMSQWRIPDVHLIELQYNIIRGHSTGAGEPLPAIYVRASMLSRLMT
                     FLQAHSGVNFQTCRLLVEFINREIYPLVPQHGSVGASGDLVQLAHVALALIGEGEVFY
                     KGERRDTAEVFAELGLEPLKMYIREGLAITNGTAVMTGIGLVNLLDAKRLMEWSLKAS
                     VLMNEIASSYDDFMSGVLNGLKHHSGQKIVASKMRQIAAGSRMLRSRENELYRGTEQQ
                     SFSNKVQPYYSLRCVPQVLGPVWDTMENAEKVLIDELNSVDDNPIVDPHSKTVVHGGN
                     FHGDYVSFEMDKLKMAVTKMTMLAERQMNYLFHDRINGILPPFVNLGVLGLNYGLQAS
                     QFTATSTTAECQTLSNPMYVHSIPNNNDNQDIVSMGTNSALICRQVIENSYQVMAIHM
                     IALAQAVDCLGAADKLAPSTRKLYEELRLLVPAFVEDTPKYKEIERIENRLRGGL"
     gene            9224..9961
                     /gene="fabG"
                     /locus_tag="PLI37_01155"
     CDS             9224..9961
                     /gene="fabG"
                     /locus_tag="PLI37_01155"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOQ57659.1"
                     /translation="MNPTKYALVTGASRGIGRAIALKLSTEGYKIIVNYASNTAAAEK
                     TLSDIKALGGSAELLQFDVSDSKATLEALQTWKSKHPEEYIPVLVNNAGIRKDNLMMW
                     MPQEDWRDVLSVSLDGFFHVTQALLKDMLVKKWGRIVNIVSLSGIKGMPGQANYSAAK
                     GGLVAATKALAQEVAKKRVTVNAVAPGFIKTDMTADLNEAELKSLIPAGRFGEPNEVA
                     ELVAFLVSDSAAYITGEVISINGGIYT"
     gene            9968..11185
                     /locus_tag="PLI37_01160"
     CDS             9968..11185
                     /locus_tag="PLI37_01160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOQ57660.1"
                     /translation="MAQRVVITGMGIYSCIGKNKEEVLKSLFEGKSGIGIVPERRELG
                     YRSALSGILEKPNLKELLDRRQRLCLSEHGHYAYVATLEALQQASISPDYLKLNEVGI
                     LYGNDSSAEAVINGVDIMREKKNTVLVGSGNIFQSMNSTITMNLSTIFNLRGINFTVS
                     GACASGSHAIGMGYLLIRQGLQDCVICGGAEEVNKYSVGNFDALSAFSIREDEPAKAS
                     RPFDKNRDGLVPSGGAATLILESYDSARKRGAGILGEVIGFGFSSNGDHISVPNIDGP
                     VRSLNMAIRGAGVKPGDIKYINAHATSTPIGDINEAKAILEVFGEHKPYVTSTKSQTG
                     HEMWMAGASEVIYSILMMQNNFIGANLNFEEPDEVSINLNIPAKRVDTAFDMFLSNSF
                     GFGGTNSTLIIKK"
     gene            11198..11443
                     /locus_tag="PLI37_01165"
     CDS             11198..11443
                     /locus_tag="PLI37_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOQ57661.1"
                     /translation="MTIEEIVEKATTILAEEFEVEESEITPEASLKETLGLDSLDLVD
                     VVVLVEQHFGVTLKGQDFVGIKTFNDFYELLNRKING"
     gene            11436..12338
                     /locus_tag="PLI37_01170"
     CDS             11436..12338
                     /locus_tag="PLI37_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOQ57662.1"
                     /translation="MAKEPEKQWEGKSRGGRWGYTFFISTIRLFGIRCAYFFLALVVL
                     YFIPFAPKATKSVWLYHRRRRKLGVLRSLRELYCHYYVFGQTLIDKVAMNAGMTNRYN
                     YVFDNYERFLDLLNSGQGVVMIGAHVGCWEAGAGFFGEYGKKINIAMFDVEHQEIKEL
                     LESNSSSKNNYKIIAINQDAISAVLQMKIALNNGEYLCFNGDRYVDKNNARAVEFMNA
                     RANFPIGPFRIAAKCGAPVVFYYAMREPARTYRFIFEEAGIEGKNSEQNITDQYVKSL
                     ERILKSYPRQWFNFYDFWNESIIV"
     gene            12342..13439
                     /locus_tag="PLI37_01175"
     CDS             12342..13439
                     /locus_tag="PLI37_01175"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ57663.1"
                     /translation="MKLSKPLEDIYGKEQQTALEAQRRAQEIAFAPMVFQVSRLMVKF
                     GILDYLAKSRNGATQQAIVEHTGLSKYAVQVLLEASLSIGTVLIKQDRYEISKAGRFL
                     LNDPMARVNMNFNQDVNYLGLFHLEEALREGKPAGLKVFGNWATIYEALSELPEHVQK
                     SWFAFDHFYSDESFAAALEVVFGSNRKINRLLDVGGNTGRWALQCVDYDKDVEVTIMD
                     LPQQLEMMRKNIAGKQGAERIKAYGCNLLDPGAKFPGPAFDVVWMSQFLDCFSEEEVV
                     SICSRAAESMDRHSRLYIMETFWDRQKYETAAYCLTLTSIYFTAMANGNSKMYHSDDM
                     LRCVGNAGLEVEKIVDGLGLGHSIMVCKKKR"
     gene            13436..13843
                     /locus_tag="PLI37_01180"
     CDS             13436..13843
                     /locus_tag="PLI37_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HOQ57664.1"
                     /translation="MNNILELIPQREPMVMVDEFLGMGEAVSKTRFTVREDNLFVDNG
                     QLSECGLIEHIAQSAAARIGYIFKSKNEPVPIGYIGSVNDFIISRAVKPGETIETTVK
                     VLQEVLNITLIEARCYINDKEVASSKMKIFIKE"
     gene            13844..14302
                     /locus_tag="PLI37_01185"
     CDS             13844..14302
                     /locus_tag="PLI37_01185"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOQ57665.1"
                     /translation="MKRNRKIGTTEPILRNITQTTVRFSEVDSMQVVWHGEYVRYFED
                     GREAFGRQFTGIGYLDFYAEGYTAPIVDLQLQCISPLRLNDQVLIETRYIDTPAAKLC
                     FEYIITRQSDSALIAKGSSVQVFIDSDGNMCLNNPEFFQNWKRKWLEKQK"
     gene            14281..15960
                     /locus_tag="PLI37_01190"
     CDS             14281..15960
                     /locus_tag="PLI37_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOQ57666.1"
                     /translation="MVGKTEVNIYIGTTSIYTCLGNKFETLAAMNRGRGGLSFNKQFS
                     MFTGSLEVEMMETYTRFESLMIDQLIEVLSRTNIKLSDPSVQLIISTTKGNVELLEED
                     VDHPSERTFLYTSAELVAARFSASNPPIVISNACISGVSSFVVARELLLSGRFNHAVV
                     VGCDVLSEFITSGFASFKSISEKPCRPYDAQRDGLSLGEACGAVVLTTDISRAVSPYI
                     RLAGGAITNDANHISAPSRTGDGLFYAIRSAMAEAGLSADDIGFVNSHGTATRYNDEM
                     ESKALALSGLNTKPLNSLKGHIGHTLGASGVVETVLAVEQLRQGYIVGTYGFKESDLP
                     HPLAISSGKQKITKACCVKTASGFGGCNAAIVLEGSDSAKLTTENHNILPQKVNVVAK
                     YTLPQSDKAFADYIRVEFKQLGESNLKFYKMSDMSKALYIAVERLLAIEGFSEIEPTR
                     RAIILANRSASIDSDIAHQQIVSQRLPEGPSPAVFVYTLANVSAGEMCIRHKIQGDNT
                     FFIENRDSGLAEDYARLLIARDRADAVICGWCEYLKGEWNVDLKLLKKIYD"
     gene            15962..16216
                     /locus_tag="PLI37_01195"
     CDS             15962..16216
                     /locus_tag="PLI37_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOQ57667.1"
                     /translation="MEQLIGELKEHLIEELNLEEITPEDIDSEAPLFRDGLGLDSIDA
                     LEIILILEKYYGIKLSNSAEAKPIFYSVKTLAEYIMKNRK"
     gene            16238..17416
                     /locus_tag="PLI37_01200"
     CDS             16238..17416
                     /locus_tag="PLI37_01200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOQ57668.1"
                     /translation="MGVVVTGVGIVSALGVGVQSNIVSMREGRSGISAHPSILKTGNI
                     LPVGELRMTNDELHRLADIPVHKHLSRTALLGIIAAREALADAKAEPSKRIGLVSSTS
                     VGGMDLTEGFFREFMKDDSAGRLRDVRMHDCGAITSAMAHYCGINAYTTTISTACSSA
                     ANAVMFGAKLIEHDVVDYVVAGGSDALSAFTLNGFKSLMILDRQACRPFDRSRAGLNL
                     GEGAGYLVLQSDKNSASNYYCRLAGYANRNDAHHQTATSAGGEGAYLAMAEALKLANL
                     SPREVSYINAHGTGTQNNDSAESAAFIRLFGENIPAFSSTKGFTGHTLAAAGGIEAAY
                     SVLSVAKGFLYPNLNFSHPIEEFGLIPNSEFSEGNNIDCVLSNSFGFGGNCSSLVFVR
                     "
     gene            17432..18244
                     /locus_tag="PLI37_01205"
     CDS             17432..18244
                     /locus_tag="PLI37_01205"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HOQ57669.1"
                     /translation="MKCYIDSVVVDSRIPADDIRELIPDALLRRRMSRLLRTAVATAV
                     SCVGGVENIASLDAIITATGWGCLTDSERFLRNMISEDEQLLNPTPFIQSTFNTVGGI
                     IALMGKNHCYNMTYVNRSHSFEDALLDSMIRVADGSSRNLLVGAFDEQTQSQYRIMER
                     MGVFRSQSPGEGSVFVRILSEKSQSSLAQLVRLDFPGNSLNETEVLERYASSSASLLL
                     FNSYRDKGLFPTASAHSFARAVELIRRGESEVVVYNEYLGATPTVSVVQCVG"
     gene            18280..18930
                     /locus_tag="PLI37_01210"
     CDS             18280..18930
                     /locus_tag="PLI37_01210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ57670.1"
                     /translation="MWASADVGGNIYLKSLCRGDRADKLVTLTFDDGPDEVMTPKVLD
                     VLQKHDIKATFFVIGSKVEKFPHIAKRIVAEGHTIANHTYSHKATFPMSSYGAVLEEI
                     QRCTQAIEKATGKLPGLFRPPFGVTNPILGKAVRSLGLQSIGWSIRSLDTIESKSREE
                     VCRRVAGSLHPGAVVLLHDRCDGADRLLEMIIAEIKRQGYNFAPIEEMLKIDVYEV"
     gene            18920..>19527
                     /locus_tag="PLI37_01215"
     CDS             18920..>19527
                     /locus_tag="PLI37_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOQ57671.1"
                     /translation="MKFKKSITFVALMLMASTLFFAQESALEQFEQELKKENTELSTI
                     KCLFTQSRMVSVLADEVNKKGQFYFQIPGNMLLSFDDGDYIKMTPSRFEIKNAGKVNT
                     TKVDSNPMLRNLSSILSACVVGDFSQISKGFTVKAESISGEWVVEMIPRRGKSASKVS
                     KILVHFDKKDMSLNLLRMEEESGDYTEYKFYNKQFNTEIEST"
     assembly_gap    19528..19627
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19628..20617
                     /locus_tag="PLI37_01220"
     CDS             <19628..20617
                     /locus_tag="PLI37_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HOQ57672.1"
                     /translation="VIDYPKNRGKGYALKLGLQRACDLGYRYAISIDSDGQHYADDIP
                     VFIDRIEQSPDSLLIGARNLTADNMPAKNTFANKFSNFWFRVETGQKLSDTQSGYRLY
                     PLEKLKNLRTLTRGYEFEVEVIVRAAWRGVKVENVPVKVFYAPAGERVSHFRPLRDFT
                     RISILNTFLVIHALLFYYPWKFARSLTLENIRKFIDTQILHSSDTNPRMAAAMGWGVF
                     CGIIPIWGYQMIFAGVSAHFLKLNKVIAIVFSNISVPPMIPFILYGSMVVGAKVLNIE
                     NQFSISNVSLESVGLGLGQYVIGSLILALISGLVVFLISLLLMTVFKRRAGNE"
     gene            20610..24449
                     /locus_tag="PLI37_01225"
     CDS             20610..24449
                     /locus_tag="PLI37_01225"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOQ57673.1"
                     /translation="MSKLFIHIFNWFERHTKVFYAVLILLVAVFAVMASRISFQENIA
                     NFFDTSGGNKNAVFQSVKAKDKIIVLFSGEDPGQIIESAEIFQEELESLIRDSLVNSV
                     TAYADEEVIAKVTSFIYEYLPIFLDDSDYRRIGEQLSKDAIDSSIGRVYALLTSPSGM
                     FVGDIVMRDPLNIATHLLQKLEQFNPDLQYEIYDGRLFTSDLSTMLIFVEPSKGMANT
                     GENDKLVSRLEKAEQQAEINGVKIDYVGGPVVAVYNARQIKQDTTITLSLAMLIVVVV
                     IFFSFRNRWSILLLVVPPIFGALFALAMVWLIQGEMSAIAMGAGAVVLGISLSYSIHF
                     VSHLNYTSSPTRIIRELATPLTIGCLTTIGAFAALMFTSSALLQDMGLFSMLALIGTA
                     LFCLIFLPHFLKRFTVTKKSFLLDKIERIVGFDRYKTNLWVVLPIVLATVVGLFFYKD
                     VKFDDDMSNINFTPPHIAEAEARSAEIFGDSSKDIYLVTGSDNIEQLTREYSSLTKLL
                     NEHLQRGDVSKVVTVNDFVVSVEEQKRRIDRWNSFWAERKTETLKQIDESAQRYGFRK
                     GAFDKFGQLLSKEYAPCTYSRSEIGDVPVISEWVSSSGETTSMLCRITVEEENKAALY
                     EQIDQLSHTAVIDRAYFSSKMVESTSEDFNYILFISSLIVFLALLISYGRLELTLLTF
                     LPMAMSWVIILAFMAIADIKFNIVNIVLATFIFGLGDDFSVFIMDGLLQDYKDGKKVL
                     GAHKTAIFFAAFTSIVGMGVLIFARHPALKSIALISVLGLSVVVLVSYTVQPMLFRLL
                     VTSQTERGGFPHTILSVLNTLYCFLYFLMGCIILQIYILILMVLPVKRKTKKLAFHKA
                     VYRFTGIFLNSVPTVRKIRENPHGETYEKPAVIIANHQSFIDILLLLSTSPKIVMVTN
                     SWVWNSPFFGWIVKYADFHHSADGYEPLAEKLKERVAEGYSVVIFPEGTRSVDCSIQR
                     FHKGAFYLAQLLKLDILPMMIYGAGQISAKKQGFYIKRGLVVIKTMKRVAYGDTSFGE
                     SYQQQAKNYRRWYIEQYRKLNNRYGRTCNAYFRDALIKNYIYKTPVLEWYMRIKCRID
                     GYYDLWDRLLPRNGKITDLGCGYGQLSFMLGLLSPERELLGIDYDRNKIDLAEHSFLT
                     KLTKLRFLYADMRSVDLPKSDAVIFNDSLHYVDSGTQEEILKKTLDCLNPGGVIVVRD
                     GDSSQKATHDKIEKTEVWSTKIIRFNKTSQELSFVSSEWMQQFATSNGVDIKIRRCDN
                     RTSETLYILSRKSDEEL"
     gene            24436..25929
                     /locus_tag="PLI37_01230"
     CDS             24436..25929
                     /locus_tag="PLI37_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOQ57674.1"
                     /translation="MKSFDVVIIGGGLGGLSCGAILSKEGLGVCVLEKHSVVGGCLQS
                     FRREGRTLDTGMHYVGSLSEGQIMHQYLKYFGVIDSLRLQKLDENGFDEFHFKDGTSY
                     CHAMGYENFIETLASQFPDERSGLKTLCKTIQDIGDLISPEILLEGKLSADGIKYMSE
                     SAYQEIRKHLRSEKLRNVLAGNCRLYGGEKSVTSLYEYGMVTHSNIQGAYAFVDGSQQ
                     LADALAEVIRSNGGEVCPNSEVLKIRLESNTVEYVETRDGERLQGKYIISSLHPVVTF
                     SLLENNTVYKKAFFTRINSLKNTHGIFSTYLLTKRGTLKYVNRNNYMFNTQDVWATEG
                     HYKGFNIPSVLMCMQPNHLSEHTEVVTLLAPMPLTYYQKWLHTKVENRGEEYREFKAE
                     FSEAVIDFVAEFYPNIRSYIDKVYTASPLTYRDYTATPLGTAYGIVKDYRNPIVTHIP
                     AKTRISNLLLTGQNLNIHGCIGTTVSSAVTSSEILGVEYLSKKIGNA"
     gene            25922..27631
                     /locus_tag="PLI37_01235"
     CDS             25922..27631
                     /locus_tag="PLI37_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOQ57675.1"
                     /translation="MRRLRVKKIWLILLSAIVGIPLVSTLILFTVGACLYHTADFLQP
                     DVEVNMEDYQLVEDSDSLRTSRYGSLYLNKYGLWEAKILGSPIERGAAYGAMGKDLLK
                     YQEDVFVRQIHQLISSERWIKFLHKFVVVFNRNMAKHIPQEFREEIYAMSLSCTDEYN
                     SYGTPYGRQLNYHAAHDIGHAMQEYMLVGCSSFAVWDEQSENGELVVGRNFDFYVGDD
                     FAKNKILLFVEPTEGYKFASIAWSGMMGVLSGMNERGLTVTINAAKGAMPLSSAMPIS
                     LLTRQILQYASNIDEAYRIAQGYETFVSESLLIGSAEDGFAAIIEKSPDNISIYRTES
                     SRIVCTNHYQSEVFSRDKNNLKNIATSDSPYRYERLNELLEELSPIDTGEAAFVLRNR
                     FGLGGKDIGLTNEKSINQFIAHHSVVFQPGRLRMWVSTSPWQLGEYVCYDLNEVFGKE
                     LPSGYSYALDEKNIAADTAALSSEYHRVILYKEQYKAVKEATLHKGALEQKQIEEFVS
                     NNPNYFDTYNALGDFLIAIDETEQALSCFQKALTLEISGMSERKEIEKKIQKYDKRHG
                     KRL"
     gene            27615..28928
                     /locus_tag="PLI37_01240"
     CDS             27615..28928
                     /locus_tag="PLI37_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ57676.1"
                     /translation="MVKDCELHYKDSTEIKRFQELRLRETLAYLAAHSPFYKRMFSEQ
                     NIDIDSIKTIEDMQRLPTTSKQDLQMHNFDFLCVDLLDVIDYVTTSGTLGEPVTFALT
                     ENDLRRLAYNEASSFTMAGCSKKDVLQLMTTIDRRFMAGLAYFLGARELGCGVVRVGN
                     GIPELQWDTINRVGATACIVVPSFLTKMIEFAEKNGIDPNSSSLKRAVCIGEALRLPN
                     GELTTLGKKINEKWQSLELFSTYASTEMQTSFTECEIHRGGHVPADLVIVEMLDENRQ
                     PVSNGEEGEVTITTIGVEAMPLLRFRTGDIAVRFDEPCRCGRKSPRLSSVIGRKGQMI
                     KFKGTTVYPPVLFDILDNIAEVENYIVEVYTNSLGTDQIQIRVGSNNHSDAFVKQIKD
                     VFRSKIRVAPDVKFEPVELIAKLQMPPMSRKVVKFFDLRNNNTVK"
     gene            29017..29571
                     /locus_tag="PLI37_01245"
     CDS             29017..29571
                     /locus_tag="PLI37_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57677.1"
                     /translation="MKKIFLFLLCATGFTCLNAQSDEGKYYVYGVDFTQAKVYAAQES
                     PQQFAVAFEKINRLLIMEPRKYDFSRMLGRIPEVIIEPMLKRNSATDFQNLITLTPRG
                     KDIDYPAIIGSYSLPQTEGTGVVLIAKLLDKPNKEATYDLITFNIATREILSKKEVVG
                     KVRGFGLRNYWASSIYRILKFNEM"
     gene            complement(29580..32963)
                     /locus_tag="PLI37_01250"
     CDS             complement(29580..32963)
                     /locus_tag="PLI37_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOQ57678.1"
                     /translation="MTRIFLKKHFTKLLSALLLSLAVAVQAEDVAVKTGDYTPDWESL
                     SGWDCPEWFKDAKFGIWAHWGPQCFPEAGDWYARFMYYPGSGQYNYHVANFGNPSAYG
                     LKELCHNWKAENWDPERLVNLYKSVGARYFMALGNHHDNFDLWNSPYQEWNSVNVGPK
                     KDIVKGWSDACKKAGLPLGVSIHASHAWTWLEPSQAYDGNLTKEDGYTLNNDSTEKWW
                     KGLDPQELYAQRHTHSTGWNNSGTIHSQWNWGNGASQPSAAYKTKFQNRVLELINDYN
                     PDMLYFDDTAMPFYGCDDAVGQNILAHYYNHSAGLNGGIPNVVVTGKQLTTEQKNYML
                     WDVERGIPDRVQQKYWQTCTCIGDWHYNKSTYNNGSYKSATQVVNMLVDIVSKNGNLL
                     LSIPVKGDGTIDHKEEAVLAGIKAWMDINSTSIYGTRPWKTFGEGPLAEAANPLSAQG
                     FNEGNNYSSRDVRFVQRNDTVFATIMRWPASETFSFASFSMTSEYYSGKVESVQLLGY
                     GDVAFTEHLEGVTVTLPETRPNEIAPVFRFVFDNSEVTLPEIIGAYETRADQLMSRTG
                     RNTGNYIKGSILAFKEQLEAAKSHLNDSVAAQKVIINQLREAYKTLKTTGKHQPGGPE
                     TGHLADLTTAQLIQAGGFTASTQGTRFGTPKNWTVENFRISQRNSAAGVKNGIDNFPK
                     YNTLSLGIWSGDDVEPYTCDLTNARIYRRVRLEPGRYYFGSTFEANYQMSDKSYIFAA
                     KTVLATDKVEDEALAFAKINRGGTGKFYGIYFTIDQAQDIVLGFQANLTEGANQQEFR
                     IQAVKLLSYGIVDLAALNRLIHEADAALANIIINENTGYYKRETALELQATIDAAGLV
                     TEESPDDELADAFEQLKHALNTFLENGKNPGGHPDTENAIDITTDKLTEADHFTRADK
                     SVTTRFAAPKYWTVENFRIPNGSDGVKAGLDKYDGYDCLYLGLWNDRSSNQAGNLANA
                     RIYQKVSLMPGRYYFGNTFSALYQLNKAYLFASDSLLTTNEIETKSIAFEPLMNGVRG
                     DGQYYGIFFTLEEEKDLYLGFQADLATGSNTQEFRASSVILYSYTPPSGIDRIKPEPP
                     STSEAPSYYTITGIKLQSPPEHGFYIVRQGNKTYKAFRR"
BASE COUNT         8974 a         7296 c         8128 g         8582 t
ORIGIN      
        1 acggttaata atttttttac catgtacgaa tggcgttgtt attcgtcgga agatatggtg
       61 aactggaccg atcatggtgc cgtagcctct ttgaaaaatt ttaaatggat ggacaggact
      121 aatggtgcat gggctccgca ttgtatcgag cgaaacggga agttttatct ctatgttccc
      181 attcacggcg aaggtattgc tgtgttggtg tccgattctc ccaccggccc cttcacagat
      241 cccataggga agcgcctggt cgaaagcgac catatttggc aggatatcga tccgatggtt
      301 gctatctatg atgggcaggc ttatctatac tggggaaatc ccaacttgtg gtacgtcaaa
      361 ttaaacaagg atatggtttc gtacgacctt ggcatcggag ataaaggtat tgtttccgtc
      421 gatatgaccc ccgaagcttt tggttcccaa aaaggccccg gagaccgggt tagaaccact
      481 tacaccgaag gcccctggtt atacaaacga aacaagacgt attatatggt ttacgcctcc
      541 tgcggaatcc ccgagcgcat ttcctactca acctcaacgt cgcctgaggg tccctggacc
      601 taccggggcg ttattatgga ccgggccgat cacctggctt ttaccattca cccggccatc
      661 atcgacttta aaggaaattc gtatttcttt tatcacgacc aggttttgtc cggagggcag
      721 ggctttaaac gttcggttag tgttgaacaa ttcgatttca acagcgatgg aagtattccg
      781 ctcattcctt caacaaggga gggtgtaaaa caagctctgt cggtcttgaa acccttcaaa
      841 aaggtcgaag ccgaaaccat ggcctggagc cggggcttaa agaccgggag cgacagcctg
      901 acaggggttt acgttaccga tatcaacaac ggcgatttcc agctaatacg aaatgttgat
      961 tttggcagag gagcaaaagt atttcaggcc agtctggctt ctgactccga tggaggcctg
     1021 attgaaatcc ggctgggtca tcccgaaggc gaacttatcg gcacactcga agtgctcaac
     1081 accggaggtc ggcaacaatg gaaaacgctg agctgcgaat taaaaaagat caatggcgtt
     1141 cacgacctct gctttgtgtt taagggcggc gagggagcat tgttcaattt tgactactgg
     1201 atgtttaaac ggaattgatt tattgttggc atgtatatac gtgcaacatt ttgttccgag
     1261 gcagaaacaa tccggaatag agtaaattaa tctttaccgc ttttgacctg acgcccgtct
     1321 cgctcgatag aaacaaatcc acaaaagggt aaattaaacc ccgccccgca cgcccataag
     1381 cccagcgggg tggggatttt tatgatgcag gtgttaacgt aattaacaaa cagtattacc
     1441 catgttagga cgcagaacaa gataaatcgc agaacagcaa gtagaaaagc ataaaaacac
     1501 aaaggaggaa aaagatcaat ttgttgaatt tcagggtcag aaatgcaaat tgaacaaata
     1561 taacatacct tctcatttta taagactaaa tcgtttaaat aaagtctaca tttatatcag
     1621 tttcaggcaa gtagagaaac aagcagccaa ccttaaatca attcttaatt taaatcgtga
     1681 aggtatgaga aaattaattt tacttcttgt gagtggtttt gctctttcgt tttctttgtt
     1741 agctcaagtg agaattgagg gacgcattac cgatcagtcc ggcgaagctt tgcccggagt
     1801 gaatgtggtc gaagacggga ccaacaattt tgccgtctcc gacgtcaacg ggaactatgt
     1861 tttgacggat gtcaacagaa attcaaccct gattttttct tttgttggga tgttaagaga
     1921 agaaattcag gtggggaaca gaacccgcat tgacgttgta atgagagaag atgccgtgag
     1981 tctcgaagag gtcgtgacca tcggttacgg caccgttaaa aaacgcgatc ttaccggagc
     2041 tgttcaatct atcaagagcg ccgaaatcac ccaaattcct acgggcaaca tgatgcaggc
     2101 tctgcagggc agggtagccg gtctcgacat agtccgcaga tcgggaaggg ccacttcggg
     2161 cgtcgaaatg gttttgcgtg gaaagagatc catcagcggc agcaatacac ccttgtttat
     2221 tattgacgga gtgattggaa actacgaaga catcaatccc aacgatgtgg agtcgatcga
     2281 aatcatgaaa gacgcttcct caacggccat ttacggttcg gcgggagcaa acggagttat
     2341 tattgtaacc accaaaaaag gtcaaaaagg caaagccgtt gtcaatgttg attcttatta
     2401 cggtgtcaac ggatttcttc aatttcctcc cgttcgtacc ggcgaggatt acatcgaatt
     2461 gaggcgtcag gccaacttga ccaccggagc ctgggcaaga ggtgatgctg attccaaact
     2521 gttctcggat cccgaatggg atgccatcca aaaaaatcaa tgggtagatt ggtttgaact
     2581 gggcacaaga gacggagttt tgcaaaacca ttccgtttcc gtgtcgggag gcagcgaaaa
     2641 tacgatcggt tatttttcgc tcaactacta caacgaagaa ggtatcctca aaagcgacga
     2701 catttctaag tattcgttcc gtgccaacgt agaacatcag gccaaaaaat ggttaaaagc
     2761 cggactcaat atcaacggag cttttacaga ccagaacgaa agaaaaggcc agtactttac
     2821 ccgcgttttg tgcctgctgc ccctgggtac gccctacaat gaagatggaa ccatcaatcc
     2881 gtttcctctt gccggagata cccagctgag ccctattgcc gacatggccg aagatcagta
     2941 cgtaaacaac cggattgctc tgggcgttaa tcccacttct tatctgcaaa tcaatcccat
     3001 caaaggactc actgccaaat cggtgtttag cgcctacctg aatttttcga gacaaggact
     3061 gttcaaagga gctttgtcgg ccgacggtta cggcgaaggt aagagctcgg cgcagatcat
     3121 caacggcaac agatacaatt acaagtggga aaacattgtc acttacgatt tcagcatcga
     3181 cgatgtacac aatttcggaa taaccggagt taccagttgg tccgaaaatc agggagaaaa
     3241 tgccagtatc ctgggctaca atctcaactg gaacaaatac ctcttctaca acctgggagc
     3301 taccgatatg cagagcaggg tagccacctc ttcgtatgta cgcacccaga tgatgtcgta
     3361 tgccgcccgt gtcaactaca actacatggg gcgttacttc ctgaccgttt ccaaccgttg
     3421 ggacggagcc tccattttgg ccgaaggcaa taaatgggat tatttccccg ctgccgccct
     3481 agcctggaga atcagcgacg aaggatttat gcagggcctg gacaagctcg acaacctgaa
     3541 gcttcgtttg ggttacggtg ttaccggtaa cgccggtgct tcggcttatg ccaccctaaa
     3601 cttcggagtg gccggatcaa acctggcttt ccaagaaacc ccggctcctt actacatgtt
     3661 ttcacaaaat ttggccaatc aggtactcga ctgggaaaaa tcctacaact ggaacctggg
     3721 tatagatctg aatatgttta aaaaccgttt gaacattact acagaacttt ataacacaga
     3781 caccaaagat attctgttcc agagaaatct gccggcttcc accggcggtt acaaggcagg
     3841 taattattcc atttgggaaa atgtctgcga aacccaaaac agaggtatag aatttgaggt
     3901 gaataccgtc aatatcaaaa gcaataattt cagctggtcc accaccctgt ctttggcagc
     3961 caaccgcgaa aagattacca agtttgtcag cgaagatcct gtgaccaacg gcagcaccta
     4021 tttggttgaa ggctatccca ttcagtcgtt ttatgattat aaatacctgg gcatctggca
     4081 agaagacgaa gctaccgaag ccgctttgta cggcagagta cccggcgatg tgaaaattga
     4141 agaggtgctt gtggacagca cctatactac cgccgaccgt caggtaatcg gttctcccac
     4201 gccaaagctt atttttggtt tatccaatac ctttacctac aaaggcatag atcttaccgt
     4261 actcttggat gcccgcatcg gacaaacgat gagctacggc attttgggtt ggtacaatcc
     4321 caatggcgaa ggcaacggcc cggctgttgt cgattactgg actcccgaaa accctgaagg
     4381 cagattccct cgtccgaacg cttcttattc tcgttttgcc aatcttccgc tgggaaccaa
     4441 tagtttgttc tatatcgatg gttcctattg caaagtgcgc aatgttacgc tgggttattc
     4501 cttgccgaac aatcttctca ggaagttgaa tttgaacaaa gccagaattt acgccaccgc
     4561 gtcgaatccg gtcattttta ccaaaagcaa atacttgaag aattatgacc cggaattggg
     4621 aggtgccgac gaattcccct tggccaaaca actggtcttt ggtataaact tatccttcta
     4681 acgtaaagaa aaacagagat atatgaaaac aattaataaa ttacagattc ttacggcagt
     4741 agttttaggt ttactgctgt tcaatgcttg ttcgctggaa gaatacaatc cttcgggagg
     4801 acccaccacc gacgagtatt tttcgacacc gaccggttac gagcaactga tcaatgcctg
     4861 ttattttcct ctgaccagat cgtggacagg gggcggagaa gattacgtgg tgtttatggc
     4921 cgaatgcgga accgaccttt ggacctgtcc ccagggtgag ggttggatga aagaagtgtt
     4981 ttattatacc gggctcaacg gtggcacggc tcatctgaac gaaggctggc aaagcagcta
     5041 cgaaagcatc aactactgta atgctgccat acgttttgct cccaaggcgg gatacaccga
     5101 cgatgccgta cgtgatgcca aagtggcaga agctcatttt ttgcgtgctt tcttcaactt
     5161 ctttattgta gagcaatttg gcggggttta tttgcctaag gtggaaacga caaaaccgat
     5221 taccgacatt ccccgttcga gcgtgagcga attttatgag ctgatctttt ccgatctgaa
     5281 atttgccatg acccatttac ccaagatcca gaccgaaaga ggtagagcaa ctcgggccgc
     5341 tgcttatcat ctttacgcca aagcctgtct gcaatacgcc tcttacgatg cagccatcga
     5401 caagaggact ctttatacgg aggctaaaga cgctgctttg gaaattatca acaacaaagc
     5461 cagctacgga cttgctcttt acgccgagcc ggccgatatt tttaacgtag ccaacaacaa
     5521 agacaacacc gaggcggtat gggtggccac ccactcgaag agcagcaccc tgaatccccg
     5581 tggttcaaaa tattggaacc gggtattcaa gcagtttggt tgtcttcagg aaaacggtat
     5641 ttgcggtgta tttcacgaca tcaacagtga atacgtaaaa tttgataaac gcattatgcc
     5701 cagcctcaaa ttattggatt tgtacggccc caaggatatg cgttaccaag cctttttccg
     5761 cgaggaatat tatgctacgg ccgattatac ctggtccgaa ggccattgtt caaaatttgg
     5821 caaagacatc gccacctttg caggtatcaa aaccatagct gccgatagtc tggctatgtt
     5881 gtttacccgg gccgatatcg ccgatcccct tgccctgaat tatgcctgtt tcgatcgcga
     5941 tcttatctac aatgccgacg gctcagccaa catcaactac accaactacg gttatccggc
     6001 gctcaaaaaa tacgaagcac ctggcatgta tgccggcgaa ttacataaag cgtattcctg
     6061 gtccgacaac ctaacttatc gtctggccga aacctatctg ctggccggcg aagctctcta
     6121 caggctggac gatcctctgg cagtgaatta tttcaacgaa atcagaaatc gcgcttgcct
     6181 caaccacgac ggcagcatga atatagccat gagcgacata ggtgtcgatt ttattctggc
     6241 cgaaagagcc cgcgagcttt gcggcgaata tacccgcttt atggatttga aaagaatggg
     6301 taaagccatc ttctcaagtt acatactgag taatcccgac atcagcaaac gtcaggcttt
     6361 cgatgccgac attcattttg taaggcccgt gcccgaaaaa atggaattga actatcagct
     6421 caaccccgac gacttccaga atccgggtta ttgattagct gtcacttaaa acgttcaaca
     6481 cccggactcc tgaaagaggg agttcgggtg ttgatttttt agaatagcct ggccggtgta
     6541 atgccgggat taaaagatag aaatttcttt cacagagtct ttaatgcttg attttacatc
     6601 gttccattga aggggcacat acatttttgg caaaggatcg ctttcggcat cagcaaaata
     6661 gaccagacta cgaattacat tgtaaatagc atcggtcttg tatttcaatt tataaaattg
     6721 caacatttca ttcaatgaat aatgttgcaa taagaagtag atatcgatga aatccttttt
     6781 tgtgcccctg ttggttatgg cagcgagttt catcgaggca atatctttca tgccagcgag
     6841 aattattccg tcgtcttcga ccggagcttc gagccattca aatgggtagt ttacaatatc
     6901 aactttcact ccttcgatat cgaagatgtt aattaaagat gtgttttgtg ttgagttaaa
     6961 tgtaaatcct tcgcgattga aagcgtcggt aaaatcaata agcgaatatt cgaaggaacc
     7021 gaaaaaatcc agatcaatag aattgcggtg acccaagtgt agagccagag cagtaccgcc
     7081 aacaagccgt gcgttatgaa aaacgggcag ctgttgaagt cttttcagaa gtgccaaagt
     7141 ttgtttttcg actgtaaatg tttgtagcat cggaattgat cttcgggaat atgactcaca
     7201 gtggaaatga aagccaatga ttttggaaac agacttctca gatttcgagc aatagacgcg
     7261 atctgattca atccataatg gttgagaatt atcttccagt cttcccattg tccgtagtcg
     7321 agtacgcggt ttattatgaa agctttgtgt tgctccatat ccaagtcttc aacagttacg
     7381 tcccaaaaca aattgggtga aaattccgta ataggattct tcttgtcttc ttctttttcc
     7441 attgggaacc tgtgattatt tttctttttc aaaagtaaga agaaaaaatt gaacagaaaa
     7501 gccatttagc tgatattcat aatatatcaa aatcagtcct aaaaacattt tgagataaaa
     7561 acaaaaaatg cttacttcac ggcctaattc caggctgccc tatgtagagg gcagcttaca
     7621 ttaaggactt ttgtaaaccg tttgtattgt cttttttaaa aaatgcgcaa gaaataatat
     7681 gattattggt aagaggttaa atctggagga atttcgggcc attgtagagg gaaaagctaa
     7741 gatagatgtc gatgacgaag taaaaaaaag tgtagtagag tgttttgatt ttttggagga
     7801 tttttcgaag gacaaggtta tttacggtat caataccggg tttggtccga tgtctcaatg
     7861 gcgtattcct gatgtacacc ttatagagtt gcaatataac atcattcgcg gtcattccac
     7921 cggtgcaggc gaacctctgc cggcgattta tgtgcgcgcc tccatgcttt cccgtctgat
     7981 gactttcctg caggcacatt cgggagtgaa ttttcaaacc tgtcggttac ttgtagagtt
     8041 tatcaacaga gagatatacc ccttggttcc gcagcatgga agtgtgggag caagcggaga
     8101 tttggtacag ttggcccatg tcgctttggc tctgattgga gagggcgagg tgttctataa
     8161 aggcgagcgt agagatactg ccgaagtgtt tgcagagttg ggcctggagc ccttgaaaat
     8221 gtacatccgt gagggcctgg caataaccaa cggaacggcg gttatgacgg gaataggcct
     8281 ggtcaatctc cttgacgcga agcgactgat ggagtggtcg ctgaaagcct cggtattgat
     8341 gaacgaaata gcctcttcat acgacgattt tatgtccggt gtattaaacg gactcaaaca
     8401 ccattcaggg caaaaaatag ttgcctcaaa gatgcggcag atagctgcag gcagccggat
     8461 gttgcgtagc agagaaaatg aattgtatcg gggaacagag caacagtcgt tttccaataa
     8521 agttcaacca tactattctt tgcgttgtgt accacaggtt ttaggaccgg tatgggacac
     8581 tatggagaat gccgaaaagg ttttgataga cgagctgaat tcagtagacg acaaccctat
     8641 tgtagaccca catagtaaaa cggttgttca tggcggaaat ttccacggtg attatgtctc
     8701 ttttgagatg gacaagctga aaatggccgt taccaagatg actatgcttg cagaacgaca
     8761 gatgaattat ctgtttcacg accgtatcaa tggcatacta ccgccctttg tgaatcttgg
     8821 tgtgctcgga ttaaactacg gtttgcaagc ttctcagttt acagccacct ctacaacggc
     8881 tgagtgtcag acgctttcaa atccgatgta tgtccactct ataccaaaca ataacgacaa
     8941 tcaggatata gtatcgatgg gaacaaattc cgccttgata tgcagacagg tgatagaaaa
     9001 cagctatcag gtgatggcta tccacatgat tgctctggct caggcggtcg attgtctggg
     9061 agcggccgat aagctggctc cgtccactcg taaactttac gaggagctgc gtttacttgt
     9121 gccggctttt gtagaagata ctccaaaata caaggagata gagagaatag aaaataggct
     9181 aagaggaggg ctgtaaaccg acaacagatt gataagaatg gaaatgaacc caactaaata
     9241 tgcgttagta acaggtgcca gtcgcggcat tggacgggca attgctctaa agctctccac
     9301 cgaaggttat aaaataattg taaattatgc atccaatacg gcagcagccg aaaaaacctt
     9361 aagcgacatt aaggctctcg gcggtagcgc tgaactttta cagttcgatg taagcgatag
     9421 caaggctacg ctcgaagcac ttcagacgtg gaagtcgaaa caccctgagg agtatattcc
     9481 cgtgttggtc aataatgccg gtatccgaaa ggataacctt atgatgtgga tgccacagga
     9541 ggattggcgc gatgtgcttt ctgtttcgct cgacggattt ttccatgtta cccaagcctt
     9601 gctcaaagat atgcttgtta agaaatgggg gcgaatagta aatatcgtct cgctttccgg
     9661 cataaaagga atgcccgggc aggcaaacta ttcggctgcc aaaggtgggc ttgtagccgc
     9721 tacaaaggcc ttggctcagg aggttgctaa aaaacgggtt acggtaaacg ccgtggctcc
     9781 gggtttcata aaaaccgata tgactgccga tttgaacgag gctgaactaa agagtcttat
     9841 tcccgccgga cgtttcgggg aaccaaacga ggtggcagaa cttgtagctt ttttagtatc
     9901 tgactcagca gcctatatca cgggtgaggt gatatccatt aacggaggaa tttatactta
     9961 aggagttatg gcacagcgag tagtgattac aggaatgggt atatactcct gtattgggaa
    10021 aaacaaagag gaggttttga agtcgctttt cgagggcaag tccggtatag gcatagtacc
    10081 cgaaagacgg gaattgggct atcgttccgc tcttagcggc attttggaaa aaccaaatct
    10141 gaaagagtta ttagaccgcc gtcagcgact atgcttgtcc gaacatggac attatgccta
    10201 tgttgccacc ttagaggcct tacaacaggc ttccatatcg cccgattatc taaagttaaa
    10261 cgaagtggga atcctctacg gcaacgatag cagcgccgag gctgtgataa acggagtcga
    10321 cattatgagg gaaaagaaaa acaccgtgtt ggtgggctcc ggtaatattt ttcagtcgat
    10381 gaattcgacc atcaccatga atctgtctac catattcaat cttcgtggaa ttaattttac
    10441 cgtatcgggt gcttgtgcca gcggttcgca cgccataggc atgggttatc tgctgataag
    10501 gcaaggtctg caagactgcg ttatctgcgg tggagccgaa gaggtcaaca agtattccgt
    10561 gggaaatttc gatgctctga gtgccttttc tattcgcgaa gatgaacccg caaaggcatc
    10621 tcgccccttt gacaagaatc gcgatggtct ggtgccaagt ggaggtgccg ccacattaat
    10681 cctggagagt tacgactccg ctcggaagcg gggagccggg atactcggag aggtaatagg
    10741 cttcggattt tcatcgaacg gtgaccatat atccgtgcca aacatagacg gaccggttcg
    10801 ttcgctcaat atggcaatcc gcggtgcagg cgttaagccg ggagatataa aatacatcaa
    10861 cgctcatgcc acttctactc ccattggaga cattaatgaa gcaaaagcaa tcttagaggt
    10921 gtttggcgag cataaaccat acgttacatc aactaaatcg cagacggggc acgaaatgtg
    10981 gatggcggga gcaagcgaag ttatttactc tatacttatg atgcaaaaca actttattgg
    11041 agcaaactta aattttgagg aaccggatga ggtttcgata aatttgaaca taccggcaaa
    11101 aagggtggat acggcatttg atatgttttt gagcaactct tttggctttg gaggtaccaa
    11161 ttcaactttg attataaaaa aataaacaac cggaaatatg actatcgaag agattgtaga
    11221 aaaagcaaca accatattgg cagaagagtt tgaagttgaa gagtctgaga taacaccgga
    11281 agcttctctt aaggagacat tgggcttaga cagcctcgat ttggtagatg tggttgttct
    11341 cgtagaacag cattttggtg ttactctcaa gggacaagac tttgtcggga ttaaaacatt
    11401 caacgacttt tacgagcttc tgaatcgcaa aatcaatggc taaagaaccc gaaaagcagt
    11461 gggaaggcaa atccagaggc ggtcgttggg gctatacatt ttttatctct acgatcagac
    11521 tgtttggaat tcgctgtgcc tatttctttt tggctttagt tgtactctat tttatacctt
    11581 ttgcaccaaa agccaccaaa agcgtttggc tttaccatag gcgtagaaga aaacttggag
    11641 tgctacgatc tctcagagag ctgtattgcc actactacgt atttggtcaa accctgatcg
    11701 ataaagttgc aatgaatgcc ggaatgacaa atcgttacaa ctatgtgttt gacaactacg
    11761 aacgtttttt ggacttgctc aacagtggtc agggagtagt gatgattggt gctcatgtgg
    11821 gttgctggga ggccggagcc ggattttttg gcgagtatgg caaaaagatc aatatagcga
    11881 tgttcgatgt agagcatcaa gagataaagg agctgctcga aagtaattcc agtagcaaga
    11941 ataactacaa aattattgcc attaatcagg atgccatttc ggccgtgttg cagatgaaaa
    12001 ttgcccttaa caacggagag tatctttgct tcaatggcga cagatacgtc gataaaaaca
    12061 acgcccgggc agttgaattt atgaatgcca gagcgaattt ccctatcgga ccatttcgga
    12121 ttgccgcaaa atgcggagca cctgtggtgt tttactacgc gatgcgcgaa ccggctcgca
    12181 cttacaggtt tatattcgag gaggctggga ttgagggtaa aaattccgaa caaaatatta
    12241 cagatcagta tgttaagtcg ttagaaagga tactgaaaag ctatcctcgc caatggttta
    12301 atttctatga tttttggaac gaaagtatta tagtataaga tatgaaactc tctaaaccac
    12361 ttgaagatat atacggcaaa gagcagcaga cggctctcga agcacagagg agagcgcagg
    12421 agattgcttt tgcacctatg gtttttcaag tctctcgcct gatggtgaaa tttggaattt
    12481 tagactattt ggcaaagagt cgtaacggag ctactcagca ggcgatagtg gagcacacgg
    12541 gcttatcgaa atatgctgta caggtgttgt tggaggcatc gctctccata ggcacggtat
    12601 taataaaaca agataggtac gagatttcta aagcaggccg gtttttgcta aacgacccga
    12661 tggccagggt aaatatgaac tttaatcaag acgtcaatta tttaggactt ttccatttgg
    12721 aagaagctct tagagaggga aaacctgcag ggctgaaagt ttttggcaat tgggctacaa
    12781 tctacgaggc tctgtctgaa ctgcccgaac atgttcagaa gagttggttt gcctttgacc
    12841 atttctactc ggatgaatcg tttgcagctg ccttggaagt ggtgttcggt tcaaacagaa
    12901 agattaatcg tttgctcgac gtgggcggca ataccggtcg ctgggccctg caatgtgtgg
    12961 actacgataa agatgtggag gtaacaatta tggatctgcc acagcaattg gagatgatgc
    13021 gcaagaatat agcgggaaag cagggagcag aacgcattaa agcttatggt tgcaatctgc
    13081 tcgacccggg tgcgaagttc cccgggcctg catttgacgt cgtttggatg agccaatttc
    13141 tcgactgctt ctcggaagag gaggtggtga gtatatgcag cagggcagcg gagtcgatgg
    13201 ataggcattc gcgcctttac attatggaga ccttctggga caggcaaaaa tacgagaccg
    13261 cggcctattg tcttacgctc acaagcatct actttaccgc aatggccaat ggcaatagca
    13321 agatgtatca ttcggacgat atgcttcgat gcgtcggaaa tgcaggattg gaagttgaaa
    13381 agatcgtaga cggcctcggg ttgggacatt cgataatggt atgcaagaaa aagcgatgaa
    13441 taatatatta gagctcatac cgcaaagaga acccatggtg atggtagatg agttcctcgg
    13501 catgggagag gctgtatcga aaacccgctt tacagtacgg gaagacaatc tgtttgtaga
    13561 taacggacag ctctccgaat gcggcctgat cgaacacatc gcccagtcgg cagcagccag
    13621 aatcggctat atattcaaga gtaaaaacga gccggtaccc attggctaca taggctctgt
    13681 aaatgatttc ataatttcga gggccgtaaa gcctggagaa actatagaaa ccaccgtaaa
    13741 ggtgctgcag gaagtgctca acatcacttt aatagaggcc cgttgctaca taaacgacaa
    13801 agaagtggcc tcaagtaaaa tgaagatatt tataaaagaa tagatgaaac gaaatcgcaa
    13861 aataggcacc acggagccaa tattgagaaa catcactcaa acaacggtca gattcagcga
    13921 agtggactcc atgcaggtag tgtggcacgg cgagtatgtc cgctattttg aagacgggcg
    13981 ggaagctttc ggacgccagt ttaccggtat cggctattta gacttttatg cagaaggcta
    14041 caccgccccc atagtggatc tgcaactgca atgtatttct cctctccgtc tgaacgatca
    14101 agtgctgata gaaactcgct acatagacac gcctgcggca aaattatgtt ttgagtacat
    14161 catcacccgg caaagcgact ctgctttgat tgcgaaggga agttccgttc aggtgtttat
    14221 agatagcgat ggaaatatgt gtttgaataa tcctgaattt tttcagaatt ggaaaagaaa
    14281 atggttggaa aaacagaagt aaatatttac ataggaacaa cttccattta tacttgcctg
    14341 ggcaataaat tcgagacctt ggctgctatg aacagaggca gaggaggatt gagttttaac
    14401 aagcaatttt ctatgttcac gggttcgctc gaagtagaaa tgatggagac ttatacccgt
    14461 ttcgagtcgc tgatgataga tcagttgatc gaagtactaa gccgtacgaa tatcaaactg
    14521 tccgacccat ctgttcaact catcatatca accacaaaag gaaatgtaga gctacttgag
    14581 gaggatgttg accatccttc cgaacgtaca tttttataca cttcggcaga gttggtggca
    14641 gctcgttttt ctgcctcgaa ccctccgata gtaatctcaa atgcctgtat ttcaggcgta
    14701 tcttcgtttg ttgtagcccg cgagcttttg ctttcgggga ggtttaacca cgcggttgtg
    14761 gttggttgcg atgtgctgtc tgaatttatt acatcgggct ttgcatcgtt taaatcgatc
    14821 agcgaaaaac cttgtcgtcc ctacgatgca caaagggacg ggcttagtct gggcgaggct
    14881 tgcggagcag tagtgctaac caccgatata agcagggcag tgtcgccata tattcgcctt
    14941 gcaggaggtg ctatcacaaa cgatgcgaat catatatctg ctccctcgcg cacaggagac
    15001 ggattgtttt acgccatccg ttcggccatg gcagaggcag gactttctgc agacgatatc
    15061 ggttttgtga attcacacgg aacagctacc aggtacaacg acgagatgga aagcaaggct
    15121 ttggcattat cgggactgaa caccaaaccg ctcaacagcc tgaaaggcca cattggacat
    15181 acgcttggag catcgggcgt agtggagacg gtgcttgccg tagagcagtt gaggcaagga
    15241 tatattgttg gaacatacgg ctttaaggaa tctgacctgc ctcatccgct cgctatttcg
    15301 tcagggaaac aaaagattac aaaagcttgt tgcgtgaaaa cggcctccgg ctttggaggt
    15361 tgcaatgcag ctattgtatt ggagggttcg gatagtgcaa agcttacaac agaaaaccac
    15421 aatatactgc ctcaaaaagt gaatgtagta gcgaaataca ccctgcctca gtctgataag
    15481 gcgtttgccg attatattcg cgtagagttt aaacagcttg gagagagcaa cctgaagttt
    15541 tacaagatga gcgacatgag caaagcgctt tatattgctg tagagagact tttggctatc
    15601 gagggttttt ctgaaatcga acccacgaga cgggcaatta ttcttgcaaa ccgctctgcg
    15661 tctatcgatt ccgatattgc ccatcaacag attgtttctc agcgtttacc cgaaggcccg
    15721 tctcccgctg tatttgtcta tactttggca aacgtctctg ccggcgagat gtgtattcgc
    15781 cacaagatac agggcgacaa cacctttttt atagagaatc gagactccgg tctggcagag
    15841 gattatgccc ggctgctgat agcccgcgac agagcagacg ccgttatttg cggttggtgc
    15901 gaatacctga aaggagaatg gaatgttgat cttaaattat taaaaaaaat atacgactga
    15961 tatggaacag cttattggtg aactaaaaga gcatttgata gaagagctca accttgagga
    16021 gattactccg gaggacatcg acagcgaggc gccgcttttc agagatggct tagggctcga
    16081 ttcgatcgat gcattggaga ttatccttat tttggaaaaa tattacggta ttaaactctc
    16141 aaactctgcc gaggcaaagc caatattcta ttcggtaaaa actttggcgg agtatattat
    16201 gaagaatcgt aaataaagct cagaaaagca agccggcatg ggagtcgtag ttacgggagt
    16261 gggcatagta tcggcgctcg gagtgggcgt tcagagcaat attgtttcta tgcgtgaggg
    16321 gcgcagcggc atctctgcac atccttcgat actaaaaaca ggcaatatct tgcctgtggg
    16381 cgaactccgt atgactaacg atgaacttca tcgattggca gatatcccgg tgcacaagca
    16441 cctttcccgc actgcgctgc ttggaataat tgccgcaagg gaggctttgg cagatgcaaa
    16501 ggcagaacct tcgaaacgta tcggtttggt ttcgtccact tcagtagggg gtatggacct
    16561 cactgagggc tttttccgcg aatttatgaa ggacgattcg gcgggcaggc tgcgcgatgt
    16621 cagaatgcac gattgcggtg ctatcacatc ggccatggct cattattgtg gcatcaatgc
    16681 ctacactaca accatcagca cagcttgttc ttcggcagcc aatgcggtga tgtttggagc
    16741 aaagctcata gaacatgacg tggtcgacta tgtagttgcc ggtggtagcg acgctttgag
    16801 tgcttttacc ctgaatggct ttaagtcgct gatgatactc gaccggcaag cttgccgtcc
    16861 tttcgaccgc agccgtgccg gattaaacct gggcgaaggt gcaggttacc ttgttttgca
    16921 gagtgataag aattcagcct ctaactatta ctgccgcttg gcgggttatg ccaaccgcaa
    16981 cgatgctcac caccaaacgg cgacctcagc cgggggcgaa ggagcttatt tggccatggc
    17041 agaggctttg aagctggcca acttatctcc ccgggaggta agctatatta atgctcacgg
    17101 cacaggcact caaaacaacg attcggccga gtccgccgca tttatcaggc ttttcggcga
    17161 aaatatccct gcgtttagct ccactaaagg ctttacaggt cacacgctgg cggcggcggg
    17221 cggcatagag gctgcctatt cggttttgtc ggtggcaaag ggttttctat atcccaacct
    17281 gaattttagt catccaatag aggagtttgg attgattcca aactctgagt tttcggaggg
    17341 caataacatc gactgtgtgc tttccaattc ctttggcttt ggcgggaatt gcagttcatt
    17401 ggtttttgta cgctaagagg gaggaaagta tatgaagtgc tatatagaca gcgttgtggt
    17461 agatagccgg ataccggcag acgacattag ggaactgatt ccggatgccc ttttgcgcag
    17521 gcgtatgagc cgtttgttgc gaacggcggt tgccacagca gtgagctgcg tgggaggagt
    17581 agaaaacata gcctctttag atgccataat caccgctacg ggctggggtt gcctcaccga
    17641 tagtgaacgg ttcttgcgaa atatgatatc tgaggatgag caacttctga accccacgcc
    17701 atttatccaa tctaccttca atacggtagg tggaataatc gcacttatgg gtaaaaatca
    17761 ttgctacaat atgacttacg tcaatcgcag ccatagcttc gaagatgcac tgctcgactc
    17821 gatgataaga gtagccgatg gcagcagtcg gaatcttctg gtaggagcat tcgatgagca
    17881 gactcagtcg caatatcgaa taatggagcg tatgggagta tttcgcagtc agtcgccggg
    17941 cgaaggttcg gtatttgttc gcattctttc agaaaaaagc caaagcagcc tggctcagct
    18001 tgtcaggctc gattttccag gcaatagtct aaacgaaaca gaggttttag agcgatacgc
    18061 ctcatcgtct gcctcgctgt tgctcttcaa ttcataccgc gataagggac tgtttcccac
    18121 agcttcggct cattcttttg caagagccgt agagctgatt cggcgcggcg aaagcgaggt
    18181 ggtagtgtat aacgaatatt tgggagcgac acctacggtg tcggtagttc aatgtgttgg
    18241 atagcgatca tattcttgtt atgccttacg ctctttttgg tgtgggcttc ggcggatgtt
    18301 ggcggcaata tctatctgaa atcgctatgc agaggcgata gagcggataa gcttgttacc
    18361 ctgacgttcg acgacggacc cgacgaagtg atgaccccaa aagtgttgga tgtattgcaa
    18421 aaacacgata taaaagcgac tttttttgtg attggctcca aggtcgaaaa gtttccccac
    18481 atagccaaac gaattgtagc agagggacat accatagcca accataccta ttcacacaaa
    18541 gccacattcc ccatgagcag ctacggtgct gttttagaag agattcagcg atgcactcag
    18601 gcaatagaga aggctacggg gaagctgccc gggttattca gaccgccatt tggagtgacc
    18661 aacccaatat tgggcaaggc agtgaggtct ctgggtttgc aatccatagg ttggagcatt
    18721 cgttcgctcg acaccatcga gagtaaaagc cgggaagagg tgtgccggag agtggccggc
    18781 agcttgcatc cgggtgccgt agttttgctc cacgacagat gtgacggggc agacaggctt
    18841 ttagagatga ttattgctga aattaaacga caaggatata attttgctcc catcgaagag
    18901 atgttaaaaa tagatgtgta tgaagtttaa aaagagtata acattcgtgg ctcttatgct
    18961 gatggcttcc accttgtttt ttgcacaaga gagcgcactt gagcaatttg aacaggagtt
    19021 gaaaaaagag aataccgagc tatccaccat taagtgcttg tttactcaaa gccgcatggt
    19081 gtcggtattg gccgacgaag tgaataaaaa ggggcaattc tacttccaaa ttcccggcaa
    19141 tatgttgtta tcgttcgacg atggcgacta catcaaaatg accccaagcc ggttcgaaat
    19201 aaaaaatgcg ggtaaagtaa acaccacgaa ggtcgattca aatccgatgt tgcgcaatct
    19261 gagttctatt ctttcggcct gtgtggtggg cgatttttct caaataagca aaggtttcac
    19321 ggtaaaagcc gagtctatct ccggcgagtg ggtagtagag atgattcccc gcaggggaaa
    19381 atctgcgtca aaggtttcga aaatattggt acatttcgat aaaaaagaca tgtctctcaa
    19441 tctgcttaga atggaagaag aatcgggcga ctacacagaa tataagttct acaacaagca
    19501 gttcaacaca gaaatagaaa gcacattnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19621 nnnnnnngtc atcgattacc cgaaaaaccg gggtaaagga tacgccctga agctcggatt
    19681 gcaaagggca tgtgacctgg gttacagata cgccatcagc atagattccg acggccagca
    19741 ttacgcagac gacatccccg tattcatcga tcgtatagaa caatcgcccg actcattgct
    19801 gatcggagcc agaaacctga ccgccgataa tatgccggcc aaaaacactt ttgccaacaa
    19861 attttcgaac ttctggttca gggtagaaac aggacagaaa ctctccgata cgcagtcggg
    19921 atacaggctt tatccgctcg aaaaactaaa aaatcttcgc acattgacgc gcgggtacga
    19981 gtttgaagtt gaagtgattg tgcgtgcagc ctggcgaggc gttaaggtgg aaaatgtccc
    20041 cgtaaaagtt ttttatgcac ctgccgggga gcgtgtttca cattttcgtc cattgcgcga
    20101 ttttaccaga atcagcatat taaacacttt tttagtaata cacgcactgc ttttttatta
    20161 tccctggaaa tttgcccgct ctcttaccct ggagaacatc aggaaattta tagatactca
    20221 gatccttcac agctccgata ccaacccgcg tatggcagca gctatgggtt ggggcgtatt
    20281 ttgcggtatt atacctattt ggggatatca gatgatattt gcaggggtct cggcccactt
    20341 ccttaagctg aacaaggtga tagccatagt gttttcaaat atcagtgttc ctccgatgat
    20401 accctttata ctttacggaa gtatggtagt cggagcaaag gtgttgaaca ttgaaaatca
    20461 attctctatc agcaatgtat cgctggaatc cgtcggtttg ggacttggtc agtatgtgat
    20521 tggcagttta attttggctc tgatttcagg cctggtggtc tttttaatat ctttactgct
    20581 gatgactgtc tttaaaagaa gagccggcaa tgagtaaact gtttatacat attttcaatt
    20641 ggttcgagcg tcacactaag gtgttttacg ctgtactgat tttgttggta gcagtgtttg
    20701 ccgtaatggc ttcgcgtatc tcgttccagg aaaatattgc caattttttc gacacttcgg
    20761 gaggcaataa aaatgccgtc tttcagagtg ttaaggcaaa agataaaatc atagtgctgt
    20821 tttcgggcga agaccccggc caaatcatcg aatctgccga aatatttcag gaagagctgg
    20881 aatctctgat tcgggatagt cttgtcaatt ccgttacggc ctatgcagac gaggaggtga
    20941 tagctaaggt tacatctttt atctacgaat acctgccgat attccttgac gatagcgact
    21001 atcggaggat cggggagcag ttgtcgaaag atgcgatcga cagctctata ggcagggttt
    21061 atgcacttct gacctcgcct tcgggcatgt ttgtaggcga tattgtaatg cgcgatcccc
    21121 tgaatatagc cacccatctg ctgcaaaagc tcgaacaatt caatccggat ttacaatacg
    21181 aaatctacga tgggcgtctc ttcacgagcg atttatccac catgcttatc tttgtggaac
    21241 cctctaaagg aatggcaaac acgggcgaga acgataagct tgtatcgaga ctcgaaaagg
    21301 cagaacagca agccgaaata aacggtgtga agattgatta cgtgggtggt ccggtagtgg
    21361 ctgtatacaa tgcccggcag atcaagcaag atacaacgat cactctgagc ctggcaatgc
    21421 tgatagtagt cgttgtgata ttcttttcgt tccgcaaccg gtggtcgata ttgcttcttg
    21481 tggtaccgcc catattcgga gcattgtttg ctttggctat ggtgtggctt atacagggcg
    21541 aaatgtctgc tatagccatg ggcgcaggag cggtggtgct gggcatatct ttgagttact
    21601 ccattcactt tgtttctcac ctcaactata ccagttcacc cacaaggatt atcagagagc
    21661 tggcaactcc ccttaccatc ggctgcttaa ctaccatagg agcctttgcc gccctgatgt
    21721 ttacatcgtc ggccttgctg caagatatgg ggctgttctc tatgcttgct ctgattggca
    21781 ccgctctgtt ctgcctgata ttccttccac acttcctgaa aaggttcaca gttaccaaaa
    21841 agagcttcct tttggacaaa atagaacgta tcgtagggtt tgacagatac aaaaccaatc
    21901 tttgggttgt tttaccgatt gtactggcta cggttgtagg cttgtttttc tacaaagacg
    21961 tgaagttcga cgacgatatg tcgaacatca actttacgcc ccctcatatc gcagaagccg
    22021 aagctcgctc ggcagagatt tttggcgaca gcagcaaaga tatctacctg gttaccggaa
    22081 gcgataatat agagcaactt acccgggaat attcctctct taccaaactg ctcaacgaac
    22141 atttgcagag aggtgatgtc tccaaagtgg ttaccgtcaa cgattttgtg gtctccgttg
    22201 aggagcagaa gaggaggatt gaccgttgga acagcttttg ggcggagcgc aaaacagaaa
    22261 ccctgaagca aatcgatgag tcggcccaaa gatatggttt ccgcaaaggt gccttcgata
    22321 aattcgggca gttactttcc aaagaatatg ctccttgcac ctacagtcgt tcggagattg
    22381 gcgatgtacc cgttatttcc gagtgggtga gctcttcggg cgagacaaca tccatgctgt
    22441 gcagaattac cgtcgaggaa gagaataaag ccgccctcta cgaacaaatc gatcaactca
    22501 gccatacggc cgtaatagac agagcctact tctcgtcgaa aatggtggag tccaccagtg
    22561 aggacttcaa ttacatactg ttcatatcgt ccctgatagt ctttttggca cttttgatat
    22621 cgtacggacg tctcgaactg acgcttctga cattcttgcc gatggctatg agttgggtga
    22681 taatactcgc ttttatggca attgccgata taaagttcaa catagtcaat atcgttttgg
    22741 ccacctttat ctttggtctg ggagacgatt tcagtgtctt tataatggac ggattgctac
    22801 aggactataa agacggtaaa aaggttctcg gagcacacaa aacagccata ttctttgcgg
    22861 cctttacctc tattgtcggt atgggagtgc ttatatttgc ccggcatccg gcattgaaat
    22921 ccatcgccct gatctcggtt ttggggctaa gcgttgtggt gcttgtatcc tacacggtgc
    22981 agccaatgtt attcaggctg ttagttacaa gccagaccga aaggggagga ttcccacaca
    23041 ccattctaag cgtactcaac actctctatt gcttcctgta cttcctcatg ggttgcataa
    23101 tcttacagat atacatactg atacttatgg ttttgccggt aaagcgaaag acaaaaaagc
    23161 tcgctttcca taaggcagta tatcgtttta ccggaatatt tctcaactcg gtgccaacgg
    23221 taagaaaaat cagagagaat ccccacggcg aaacctacga aaagcccgct gttatcatag
    23281 caaaccacca atctttcatc gatattttgc ttctgctgtc taccagtccc aaaatcgtta
    23341 tggtaaccaa tagttgggta tggaattcgc catttttcgg ttggattgta aaatacgcag
    23401 actttcatca ctctgccgac ggctatgaac cgctagccga aaagctcaaa gagcgggttg
    23461 ccgaaggcta ttccgttgtg attttcccgg aaggtacgcg ttcggtagat tgttcgattc
    23521 aaaggttcca caaaggagca ttttacctgg cacaactcct taagttggat atattaccga
    23581 tgatgatata cggagcgggg caaatctcgg caaaaaaaca gggattctat ataaaacgag
    23641 gtttagtcgt tattaaaaca atgaagcggg tagcgtacgg cgacacatct tttggagaaa
    23701 gctaccagca acaggcaaaa aactaccgga ggtggtatat cgagcagtac cgtaagctca
    23761 acaaccgcta cggacgtact tgcaacgcct atttcagaga cgcattgata aaaaactata
    23821 tctataaaac tcctgtgctt gagtggtata tgcggataaa atgccgtata gacggctact
    23881 acgacctctg ggacaggctt ctgccccgca atggcaaaat tacagacctt ggttgcggat
    23941 acggccagtt atcctttatg ttgggattgc tatcgcccga aagagagctg ttgggtatcg
    24001 attacgacag aaataaaata gatttggcag aacattcgtt tttaaccaaa ctgaccaagc
    24061 tgcgattcct ttatgcagat atgcgaagcg tcgatttgcc caaatcggat gcagtgatct
    24121 tcaacgatag cctgcactac gtcgattccg gcactcagga agaaatcttg aaaaaaaccc
    24181 tcgattgttt gaatcccggg ggagttattg tggtacgcga tggagactcg tcgcaaaagg
    24241 caacgcacga taaaatcgaa aaaaccgaag tgtggtcgac caaaataatc aggttcaata
    24301 agaccagcca agagttgagc tttgtcagct cagagtggat gcagcaattc gctacttcca
    24361 acggcgtgga tatcaagata cggcggtgcg acaataggac ctccgagacc ttatacattt
    24421 taagcagaaa gagcgatgaa gagctttgat gtggtgataa tcggcggagg ccttggcgga
    24481 ctttcctgtg gtgcaatact gagcaaagag gggcttggag tatgtgtgct cgaaaagcat
    24541 agtgttgtgg gtggctgtct gcaatcgttt cggagagagg gccgcacact cgatacggga
    24601 atgcattatg taggcagtct ttccgaaggg cagattatgc atcagtatct gaagtatttc
    24661 ggcgtgatag attcgcttcg actgcaaaaa ttagacgaga acggtttcga cgaatttcac
    24721 ttcaaagacg gcacaagcta ctgccatgca atgggttacg agaattttat cgaaacactt
    24781 gcctcgcaat ttcccgatga gcgttcggga ttaaaaacac tctgtaagac cattcaggat
    24841 ataggcgacc tgatttctcc cgaaatactt ctcgagggta aattatctgc cgacggtatc
    24901 aaatatatgt ccgagtcggc ttaccaggag atacgtaaac acctgcgaag cgaaaaactc
    24961 cggaacgtct tggcgggcaa ttgccgcctt tacggcggcg aaaagtctgt cacttcgctc
    25021 tatgagtatg ggatggtgac ccactcaaat attcagggag catacgcatt cgtagatggt
    25081 tcgcaacagt tggcggacgc tctggcggag gttatcagga gcaatggggg agaggtatgt
    25141 cccaattccg aagttctgaa aatccgtctc gaatccaata ccgtagaata cgtagaaacc
    25201 cgcgacggag agaggctgca gggcaaatat attatctcct cgctacaccc tgttgttacc
    25261 ttctctttat tggaaaacaa tacggtttat aaaaaggcat ttttcacgcg gatcaattcg
    25321 cttaaaaata cccacggaat cttttccacc tatctgctta caaaacgggg aacattgaaa
    25381 tacgtcaatc gcaacaatta tatgttcaat actcaggacg tttgggctac cgagggacat
    25441 tacaaaggct ttaatatccc ttcggtattg atgtgtatgc agccaaacca cttgagcgaa
    25501 cataccgagg tggtcaccct gcttgctcct atgcctttga cttactatca aaagtggctc
    25561 cataccaaag tggagaaccg gggagaagaa taccgggagt tcaaagccga gtttagcgag
    25621 gctgtaatag attttgtagc tgaattttac ccaaacatac ggagctatat cgataaagtt
    25681 tataccgcat cgccgctcac ttacagagat tatacggcca cgccccttgg aaccgcctac
    25741 ggcattgtaa aagattatcg aaatcctatt gttacacata ttccggccaa aacccggata
    25801 tcaaatctgc tgcttacggg gcaaaatcta aacatacacg gttgtatcgg cactacggta
    25861 tcgtctgccg ttacctcttc ggaaatactt ggtgttgaat acctttctaa aaaaattgga
    25921 aatgcgtagg ttgagagtaa aaaaaatatg gctgattctg ctttcggcaa tcgtgggcat
    25981 acctctggta tcgacattga ttctatttac cgtaggagcc tgcctctatc atacggccga
    26041 cttcctgcaa ccggatgttg aggtaaatat ggaggattat cagttagtgg aagacagcga
    26101 ttcattgagg acttctcgct atggcagctt atatttgaat aaatacggcc tgtgggaagc
    26161 taaaatcctt ggttccccta ttgagcgagg tgccgcctac ggtgcgatgg gcaaagattt
    26221 actgaaatac caggaagatg tattcgttag gcaaatacac caactgattt catcggaacg
    26281 ctggataaag tttttgcata aattcgttgt cgtattcaat cgaaacatgg caaaacacat
    26341 acctcaggag ttcagagagg agatatatgc catgtcgctc tcctgtacag acgagtataa
    26401 ctcttacggt acaccttacg gacgacaact caattaccat gccgcccacg acataggaca
    26461 tgccatgcag gaatatatgt tggtgggttg cagttcgttt gccgtatggg acgagcaaag
    26521 cgaaaacggc gaacttgttg tgggacgaaa cttcgacttc tatgtgggag acgattttgc
    26581 caaaaacaaa attcttttgt tcgtagaacc cacagagggc tataaatttg cctcgatagc
    26641 gtggtccggc atgatgggcg tgctgtcggg catgaacgaa cgcggactga ctgttaccat
    26701 aaatgccgct aaaggagcca tgccgctgtc ttctgccatg cccatttcgc ttttgaccag
    26761 acaaattttg caatacgctt caaacataga cgaagcctac cggatagcac aaggctacga
    26821 aacctttgtg tcggagtcgt tgttgattgg ctcggcagaa gacggtttcg ccgcaataat
    26881 agaaaagagt cccgacaata tatcgatcta ccggacagaa tcctcccgca ttgtttgcac
    26941 caaccattac cagtcggagg tttttagccg cgataaaaac aatctaaaaa acatagccac
    27001 ttccgatagc ccatacagat acgagcgact gaatgagtta ttggaagagt tgtcgccgat
    27061 agataccgga gaggcagcct tcgtacttcg aaacagattc ggtttgggcg gcaaagacat
    27121 cgggcttacc aacgaaaagt ctatcaacca attcatcgct caccactcgg tggtgtttca
    27181 gcccggccgg ctgcgtatgt gggtatccac ctcgccctgg caattgggcg aatacgtttg
    27241 ctacgacctt aacgaagtgt ttggcaaaga gctgccttcg ggctattcat acgctttgga
    27301 cgagaaaaat atagccgccg ataccgctgc tttaagcagt gaataccata gagttattct
    27361 ctacaaagag caatataagg ctgtaaaaga agctaccctc cacaagggag ctttagagca
    27421 aaaacaaata gaggagtttg tttcaaacaa tccgaactat ttcgacacat acaatgctct
    27481 gggcgacttt ctgatagcca tagatgaaac ggagcaggcc ctaagctgtt ttcagaaggc
    27541 tctgacactt gaaatttccg gaatgagcga acgaaaagag atagagaaaa agattcaaaa
    27601 atacgataaa agacatggta aaagattgtg aacttcatta caaagacagt actgaaatta
    27661 aaagattcca ggagctgagg ctcagagaaa ctctagctta tttggcggca cattcacctt
    27721 tctacaaaag gatgttttcg gagcaaaata tagacatcga ttctataaaa accatcgagg
    27781 atatgcaaag attgccgact acctcaaaac aagacctgca aatgcataac ttcgattttc
    27841 tttgtgtcga tttgcttgat gttatcgact acgttaccac atcgggtaca ctgggcgaac
    27901 ccgtaacatt tgcgcttacc gaaaacgatc tccggcggct tgcctacaac gaggcaagct
    27961 cctttacaat ggcgggttgc agcaaaaagg atgtgttgca actgatgacc accatcgacc
    28021 gccgttttat ggccggcctg gcctatttcc ttggggccag agaattgggt tgcggggtgg
    28081 tgcgtgtggg caacgggatc cccgaattgc aatgggacac catcaaccgg gtgggagcga
    28141 ccgcctgtat agtagtacct tcttttctga ccaagatgat cgaatttgcc gaaaaaaacg
    28201 gaatcgaccc caactcctca agtctgaaac gagccgtctg cattggcgaa gctttgcgtt
    28261 tacccaacgg agaactcacc actttgggca aaaaaatcaa cgaaaaatgg cagtctttag
    28321 agctattctc cacctacgcc tccacagaga tgcagacctc ctttacagag tgcgaaatac
    28381 accggggcgg gcacgttcct gccgacctgg taatagtaga aatgttggac gaaaaccggc
    28441 agcctgtctc taacggcgag gagggcgagg ttaccataac cacaattgga gtagaggcca
    28501 tgccgctgct gaggtttcgc acaggcgata ttgccgtcag attcgacgaa ccctgtcgat
    28561 gcggcagaaa aagcccccgg ctcagttcag tgatcggcag aaaaggacaa atgataaaat
    28621 ttaaaggcac caccgtatat ccgccggtac tcttcgatat actcgacaat atagccgaag
    28681 tagaaaacta catagttgaa gtttatacaa attcgttggg cacagaccaa attcaaatcc
    28741 gcgtaggcag caacaatcat tcagacgcgt ttgtaaagca gataaaagat gttttccgct
    28801 ccaaaatccg cgtagctccc gacgtcaaat ttgagcctgt tgaactcatc gcaaagcttc
    28861 aaatgccacc catgagccga aaagttgtaa agtttttcga tttgagaaat aataatactg
    28921 tgaagtaaga cgaagattga ttttttgcaa aaacgaaata ttacatttgt tccccgtgag
    28981 ttctaactta atggtaaaca acaaaatcgt ggatatatga aaaaaatatt tttatttctg
    29041 ttgtgtgcaa ccggctttac ttgcttaaat gcacaatcgg acgaaggtaa atattatgtt
    29101 tatggcgtgg attttacaca ggccaaagtg tatgctgccc aagagtctcc ccagcagttt
    29161 gccgtggctt ttgagaaaat caaccggcta cttatcatgg agccccgaaa atacgatttt
    29221 tcccgtatgc tgggacgcat ccccgaggta ataattgaac caatgcttaa aagaaattcc
    29281 gcaaccgact ttcaaaacct cattactctg accccacgag gcaaggatat tgattacccg
    29341 gcaataatcg gaagctactc ccttccccaa accgaaggca caggagtagt gctgatagcc
    29401 aagctcctag acaaacccaa caaagaagca acctacgatt taataacgtt caacatagca
    29461 acaagggaaa ttctctccaa aaaggaagta gtaggcaaag taagaggctt cggattacga
    29521 aactattggg ccagctccat ttacaggata ttgaaattca atgaaatgtg attcaagacc
    29581 tacctcctaa aagccttgta ggtcttattc ccctggcgga cgatgtagaa gccgtgctcg
    29641 gggggtgatt gcagcttgat gccggtgatc gtgtagtagg agggggcttc ggatgtggat
    29701 ggaggctctg gcttgatgcg gtcgatgccg gagggagggg tgtagctgta gaggatcacg
    29761 ctgctggcac ggaattcctg ggtgttgctg ccggtggcga ggtcggcttg gaatcccaga
    29821 tacaggtctt tttcctcctc gagggtgaag aaaatgccgt aatactgccc gtcgcctctc
    29881 acgccgttca tcaagggctc gaaggcgatg gattttgtct cgatctcgtt ggtggtcagc
    29941 aagctgtcgg aggcgaacag gtaggccttg ttgagttggt agagtgccga gaaggtgttg
    30001 ccgaagtaat acctgcccgg catcagagac actttctgat agatgcgcgc gttggccaga
    30061 ttacctgcct ggttgctcga gcggtcgttc cacaaaccga gatacaggca gtcgtagccg
    30121 tcgtatttgt cgagtccggc ttttacgcca tcgctgccgt tgggaatccg gaagttctct
    30181 accgtccagt atttgggagc tgcgaagcgg gtggtaaccg acttgtctgc ccgtgtaaaa
    30241 tggtcggctt ccgtcagttt atccgttgtg atatcgatgg cattctctgt gtcgggatgg
    30301 ccgccgggat tcttgccgtt ctcgaggaag gtgttcagtg cgtgcttcaa ctgttcaaac
    30361 gcatcggcca attcatcgtc ggggctttct tccgtcacca gtccggcagc gtcgatggtg
    30421 gcctgaagtt ccaaggcggt ctctctcttg taatagcccg tgttctcgtt gatgatgata
    30481 ttggccaggg cggcatcggc ctcatggatg agcctgttta atgcagccag gtcaacaatg
    30541 ccgtaagaga gcagtttgac agcctgaatg cggaactcct gttggttcgc accttccgta
    30601 aggttggcct ggaatccgag cacgatatcc tgtgcctggt cgatggtaaa gtaaatgcca
    30661 tagaatttcc cggtgcctcc ccggttgatt tttgcaaaag caagcgcctc gtcttctact
    30721 ttatcggtgg cgagtaccgt tttggcggcg aagatgtagc tcttgtccga catttggtag
    30781 ttggcttcga aagttgatcc gaaataataa cggcccggtt ccagacgtac cctgcggtaa
    30841 atgcgggcgt ttgtcaggtc gcaggtataa ggctccacgt cgtcgcccga ccagataccc
    30901 agactaagtg tgttgtattt cgggaagttg tcgatgccgt tctttacacc ggctgcagaa
    30961 ttcctttgag atattctaaa attctctacc gtccagtttt taggagtgcc gaagcgtgtg
    31021 ccttgcgtgc tggccgtaaa tccgccggct tgaatcaact gtgcggtggt taaatcggcc
    31081 agatgacctg tttccggtcc acccggttga tgcttccccg tagtcttcag ggttttatag
    31141 gcctcacgaa gttggttgat gatcaccttt tgggcggcaa cactgtcgtt aagatggctt
    31201 ttggccgctt caagctgctc tttgaaggcc agaatacttc ctttgatgta attgccggtg
    31261 tttcttcccg ttcggctcat caattggtcg gcccgggtct cataagctcc gataatttcg
    31321 ggcagtgtaa cttcgctgtt gtcgaataca aagcggaaaa cgggagcgat ctcgttgggc
    31381 ctggtttcgg gaagggtcac tgttacgcct tccaggtgtt cggtgaacgc cacatcgcca
    31441 tagccgagta gctgaacgct ctccaccttg ccgctgtagt attccgaagt catggagaag
    31501 gaggcaaagc tgaaagtctc gcttgccggc cacctcatga tggtggcaaa gacggtgtcg
    31561 ttcctctgta cgaagcgaac gtctctcgac gaataattgt tgccctcgtt gaatccctgg
    31621 gccgaaagcg gattggctgc ctctgcgagc gggccttcgc caaaggtttt ccaggggcgg
    31681 gtgccgtaga tactcgttga gttgatgtcc atccaggctt tgatgccggc aagtactgct
    31741 tcttccttat ggtcgatggt gccgtcgcct ttaacgggta tgctgagcag caggttgccg
    31801 tttttcgata cgatgtccac cagcatgttt accacctggg tggcactctt gtagctgccg
    31861 ttgttgtatg tgctcttgtt gtaatgccag tcgccgatgc aggtgcaggt ttgccagtat
    31921 ttttgctgaa ccctgtcggg aatgccgcgc tccacatccc aaagcatgta gtttttctgt
    31981 tctgtggtaa gctgttttcc tgtaaccact acattcggta tgcctccgtt taggccggca
    32041 ctgtggttgt aatagtgagc gagaatgttt tgtcccacgg cgtcgtcgca tccgtagaac
    32101 ggcatggctg tgtcgtcaaa atagagcatg tcggggttgt agtcgttgat aagctccagc
    32161 acacggttct ggaatttggt tttgtaggct gctgagggtt ggctggcacc gtttccccag
    32221 ttccattgac tgtgaatcgt gcctgaattg ttccatcccg tggagtgggt atgacgttgg
    32281 gcatacagtt cctggggatc cagccctttc caccattttt cggtgctgtc gttgttcagc
    32341 gtatagccgt cttccttggt gagatttccg tcgtaggcct gcgagggctc aagccaggtc
    32401 catgcgtggc tggcatgaat gctgacgccc aggggcaggc ctgctttttt acaggcatcg
    32461 ctccatccct tcacgatgtc tttctttggc cccacattaa cggagttcca ttcctgatag
    32521 ggtgagttcc acaggtcgaa attgtcgtga tggttgccga gtgccatgaa atagcgggcg
    32581 cccacgcttt tgtagaggtt taccagtctt tcgggatccc agttttcggc cttccagtta
    32641 tggcagagct ccttgagtcc gtaggcggac ggatttccga aattggcaac gtggtaattg
    32701 tattgtccgc tgcccggata gtacataaaa cgcgcgtacc agtcgcctgc ttcgggaaaa
    32761 cattgaggcc cccagtgtgc ccagatgccg aacttggcgt ccttgaacca ttcgggacag
    32821 tcccatcccg acagactttc ccaatcgggc gtataatcgc ctgtttttac ggctacatct
    32881 tctgcctgca ccgccacggc gagtgacaac agtagggcag ataacagctt cgtaaagtgt
    32941 ttttttagaa aaatgcgtgt catttcgttt ggtgtttatt ctcgcaaaga tgacatattg
    33001 cacgctaaaa atcaaacgaa acttattaaa tgttccattt gcgtaacggg gaaattttat
    33061 attctgtcta ttgccctaaa
//
LOCUS       DAOSTD010000010        32157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1535_length_32157_cov_15.093577, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000010 DAOSTD010000000
VERSION     DAOSTD010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 32157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32157
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1535_length_32157_cov_15.093577"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLI37_01255"
     CDS             complement(<1..278)
                     /locus_tag="PLI37_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOQ57679.1"
                     /translation="MGYVFLYGFCRLLAFLPLNMLYLLSDMILYPLVYYLAGYRKKVV
                     RNNLAASFPDKSDKERRSIEKDFYKHFCSVIVETIYLAGISTEEAKRRT"
     gene            complement(349..1677)
                     /gene="mtaB"
                     /locus_tag="PLI37_01260"
     CDS             complement(349..1677)
                     /gene="mtaB"
                     /locus_tag="PLI37_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOQ57680.1"
                     /translation="MTNQVTTPSPTYLFYTLGCKLNFAESAGLGKKMQQVGYQPVKVG
                     REPDICVINTCAVTGTAEQKCRQNIHRLIRKYPKAFVVVIGCYAQLRAAELAAIPGVD
                     LVLGTDEKMDILQYLRSSKLEKAEVFSGPNSGIHRFFPACARGDRTRYFLKVQDGCDY
                     FCTYCTIPFARGRSRNIGIEEAVRQAHEVAAEGGSEIVLTGVNIGDFGKSSGEKLIDL
                     LRALDGVEGIERFRISSLEPDLLSDEIIEFVAASARFVPHFHLPLQAGSNEMLRLMHR
                     RYDREHFASRVAEIRQSMPGAFIGVDVIVGMRGETQERFEESRSFLESLDISQLHVFS
                     YSERPGTQALKIQPKVSSFELKRRSEILHSLSETKRLKFYGERRGEQAVVLFEKAKKN
                     NLIHGFTENYVRVEAPWRSDWENRSIPLTLGDFNADRSALQAVPIPCGLS"
     gene            complement(1912..2355)
                     /gene="rplI"
                     /locus_tag="PLI37_01265"
     CDS             complement(1912..2355)
                     /gene="rplI"
                     /locus_tag="PLI37_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOQ57681.1"
                     /translation="MEVILKEDVINLGYKDDIVKVKDGYGRNYLIPNGLAVMATESAK
                     KVLAEDLKQRAHKLEKMKADAQDMVSKFEGLHLSIGAKVSSTGKIFGSVGPIQIAEAL
                     EQQGLEVDRKSILLKEAVKEVGEYKATVKLHREVQVEISFEVVAE"
     gene            complement(2370..2639)
                     /gene="rpsR"
                     /locus_tag="PLI37_01270"
     CDS             complement(2370..2639)
                     /gene="rpsR"
                     /locus_tag="PLI37_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOQ57682.1"
                     /translation="MAQNSSEIRYLVPPTVDTKKKKYCRFKKSGIKFIDYKDPEFLKK
                     FLNEQGKILPRRLTGTSLKYQRKVAQAVKRARHLALLPYVTDLMK"
     gene            complement(2643..2987)
                     /gene="rpsF"
                     /locus_tag="PLI37_01275"
     CDS             complement(2643..2987)
                     /gene="rpsF"
                     /locus_tag="PLI37_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOQ57683.1"
                     /translation="MNNYETVFILTPVLSEPQMKEAVEKFKNLLVAEGAEIVNEENWG
                     LRKLEYPIDKKSTGFYQMFEFKANPEVVAKLEVNFRRDESVLRFLILKQDKFFAQYAE
                     KRRSLKQAKKEN"
     gene            3202..3384
                     /locus_tag="PLI37_01280"
     CDS             3202..3384
                     /locus_tag="PLI37_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57684.1"
                     /translation="MKKILILLLKNLGIIIVLAGTVVLAATQFKGILTNTWLLVAAGL
                     FVLGLITQIFINKRVE"
     gene            3501..7787
                     /locus_tag="PLI37_01285"
     CDS             3501..7787
                     /locus_tag="PLI37_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOQ57685.1"
                     /translation="MKATSSLRKSLFAYIIAFFLTPAEFSLSANSRVKPFVTYNHYSS
                     DDEFLQGSITYLLQDRRGLIWVSSFNGLNKYDGYQWTKYKSRPGEKSLLNTNRINYLW
                     ENSSGNFWCEAGNQIYLFDVEKEEFTNLQTVFEQKYLRNVTIESIFPLGNGVSYMISE
                     VGECFRIVDSDPLNKCREIAIKASASKDVFLVFLDPQGFEWIFTKGGTYRYDTDQLVS
                     KIPFRFPAVTEDELWLTSADGRQMGIYSYQNANITYLDFIPFNGYVYKQHFKDSLIYT
                     ATGAGFYLTSIADRQTLCLSKEILQTFFVDSRGCVWGLTTDKDIVVINTKPDSGETVE
                     CQIVELPTDHHFSDPRVSFYEDGSGRIWAIFYDTNDILYYNEGTGRFERPISPEGMIT
                     NTQGVAIDKQGNLWYRTNRHIDMLTLHNQPFEVINSFPGEEVRSMLKDSKDRYWFGLR
                     ENRVLCFRKNRLVHEIPIEGTAYCLYEDSYQRIWIGSKPGGLYCLEFTEGSDNYRLSR
                     YQHDDNDPKSLSHNDVYSILEDSRGHLWIGTFGGGINLFKQGIFHHQNNHLGYDSELP
                     EAVRFICEIRPGLMLIGARNGLFSFNNLFDHPENIRMFRNTRRSLDINSLPDNDVMSI
                     LVTRDSSIYLTTSSGGICRILSTTDDLLSDRIRFESITKEDGLASDVAYSIIEDSQND
                     LWITGGRALSRLQRHEVSDNSGLQSPGIITIYDRTSFPSEVIFSEGHPLTVGNIMIFG
                     TMNGIVTIRSSQVLKDIYTPPLIVSSKSKDLKSKHQARAASIRFAALDYHDKLHLKYA
                     YKLDGVDKDWNITTENTVTYINLPPGNSLFHVKSTNADGIWTDNEQTMLIYVRPLFHE
                     TLPGRLILISAVLLFIALVVYYFQRMYRLRHRLILEQESTDTKLRFFTDISHELRTPL
                     TLIDGPVAEVLEDKQLSEQSRYYLEVVQKNVRRLLNLVNQILDFRKLQHNKITLLIEP
                     LDVKVLLSRIMENFAELANHHRILFNLNCPNDLPVIWADRDKIEKIFFNLLSNAFKYS
                     NDGAAISINVSKLNNSFNGDVEPSVVISVTDQGKGIKREDVEQLFSRFETVIQDNLFR
                     PSSGIGLSLVKQFVDLHHASIVVDSQPGKGSTFSVTFRGGKKHFENDKNVEFYAGNLS
                     ENIHQEQPAESHQEDKTEEERSKILVVEDNEELRHFICRILAADYRVVCANDGEEGLR
                     MGKMEWPDLIITDIMMPKMDGFEMIRHIKADADIYAVPLIVLTAKGEMDDRIYGVEMG
                     VDDYVLKPFSASYLKARVKALLEQRRQLQKHFLEILSQGGNALARRAIEPNMPVITPA
                     DELFIQEALAFMEKNMDNAELTIDQFADALNMGRTVFYNKLKTTLGLTPIDFVQEMRI
                     KRAVQLMKSGDFTIAEIAYQTGFNDPKYFSRSFKKHLGSSPSEYIRSIRK"
     gene            complement(7846..9687)
                     /locus_tag="PLI37_01290"
     CDS             complement(7846..9687)
                     /locus_tag="PLI37_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOQ57686.1"
                     /translation="MMLNRKINRVIKTALVCFPVAIISALLLAGNVYAAGEVFPKIPV
                     DGQKEVPVNAQIILQSTVGLIQGPDSCFLNGEYIAPASIAGGMAIFKPGQLQFATTYT
                     MLVRDGAFLSKVDNRPLAGGSYSFTTCSPKAKVFDAVVAKDGSGDYTSIRQAVKAAPN
                     NRTEPWLIFVRNGVYEELVRTSTSQPNICLVGEDKEKTVLKFSITSYGGHERNNSLFP
                     EAEGQGPVLVANSSDFYLHNITVINSWGYDNQAGPQALALGSYADRFTMFNAVLKSYQ
                     DTWQTGRDEHRHYAFRSYIEGAVDFIYASGNCVFDSCTIALCRNGSVVVAPSHGGGVK
                     YGYVFRDCNVVSSKPGTARTNNYWGRPWHNRPRTSFINTSLSKDINLSPAGWIDHMGG
                     LPVVFAEYNTIDHMGNPVSLASRNTYYWTGSDRNNPTETCIAQAVLSKEEADALTVRT
                     VLSGTDGWKPDNITTELPAPLLYYNENRLYWTAEPQAICYEVLYNGDFLTFTTETSLT
                     FEGDVSGYRVRAVNMYGTLGQVSDPPFVNGIQTETADALNVVFDGSRLLASGFCGKAR
                     VELYAIDGSKAGQYEIEENVRLDLGSVRFFIAKVIANTGICVLKAVR"
     gene            complement(9760..11991)
                     /locus_tag="PLI37_01295"
     CDS             complement(9760..11991)
                     /locus_tag="PLI37_01295"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57687.1"
                     /translation="MKKFTLLFCSLLLAFVASANVIVVDSVSNYATKYGATADGDTLL
                     LYSGTYANQNFPVGKAITILAPDTCEVFLTGEIRGEGVGDGSGLYLENITIGDGASYL
                     FNFAPVGTIKAISLKNCMIQNINRCTFYLGGDQASSAVELVEFEGCTFYNNNTGNWNF
                     SWHTTPIYKWIMNECTFYKNDGVESFYNPRGTYDGKSHEFIFTHNTLYQGCRDANRYF
                     CQVTNKFNGEDSKLVFTDNIIICPDGKVGGKLFSISAGYWDVTIKNNLIHGWEIPTLA
                     EELGDVSMENNYSLADLEIPSAAALFADPANGNFSLYKGVTPVENKSTTGGVLGAQKW
                     LKDAGQLYNLTHGLAAGVDSLAGTISGPSGKIPEGENVTLTAVKNYGFKFVKWVDENN
                     ATLSEETSYSFEMTGDKYVYAVFDAIPLFRLNITCKGGGSYVISETGKDGAYEYYEEG
                     TELVITSKTNLITEFVMGYTDDMSEQFYAPEFSIVMGKDYTIVLEFVQKDFICGWDFD
                     TQRSAVNQQRPADFLSRNYQDTAHVPTLDMYYTLYPDAPWTKTWWNRTDTYTAAVTWL
                     RLTDSGDGTEYKTSDGAMDSSKYYTDQGFYWQTAVCTRGYMSDIKYCFSIRRTYMGHY
                     DYFVQYSYDNTSWETVDTVTTTGGWQDFEVELPNTAAKELIYIRLIPDVTSGYDTNRI
                     FDVYGVYVANIFLLADYDPNAVVAPVREEQEDMIYDLMGRRLKSADRPGIYIINGKKY
                     LIK"
     gene            12185..13849
                     /locus_tag="PLI37_01300"
     CDS             12185..13849
                     /locus_tag="PLI37_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOQ57688.1"
                     /translation="MNKYLLLILHLCFCAANFSLMADTIPAFPGAEGHGRFTTGGRGG
                     LVYHVTSLEDDPVNPKFGTLRYFLNKTEKRTIVFDVSGTIFLKDALNINAGNLTIAGQ
                     TAPGDGICLAGYPVHVNSSNIIIRFLRFRMGDLDPNNEDDALGGRGIKNIVIDHCSCS
                     WSVDECVSFYGNENLTLQWCIISESLRHSNHEKGNHGYGGIWGGANTSFHHNLLAHHS
                     SRTPRLGAADNTDGRDNVDLRNNVIYNYGLVVGCYGAEGMDANIINCYYKPGPSTKTN
                     HNRRSMIIGIGANTTDSARPKWGKYYIAGNWCSSLSDTYKLPTDSYERNTCLDNWNYG
                     VYNNISEPISAQEKAAMRLSEPNNPGLVTTHTAQVAFEKVLGTAGCSLQRDEIDLRIV
                     DETRRGTATFKGSYTAVGGIIDSQNDLKPAGAGSDWSPWPTLNSTEPPKDTDRDGMPD
                     AWEEARGLNPRYPLDRNDIAENGYTNLENYLNSLVEEIVRQELSDYEFVTGSGQNDLP
                     AAINPPIVAKQKKENNKIWYDLSGRPIESPNARGLYIRRGEKLVIR"
     gene            13938..16280
                     /locus_tag="PLI37_01305"
     CDS             13938..16280
                     /locus_tag="PLI37_01305"
                     /inference="COORDINATES: protein motif:HMM:NF027701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOQ57689.1"
                     /translation="MSSCNDHMSEYYARPDWIKGNCYEVLEMDGNYTIFLKGIDKAGF
                     HDMVDGKSIVTVMAPNDSAFSLYLAEQGLNSIEEMDSSELDKLIGFHLMYYAYSADKL
                     INFRPEEGDDVTDEEKKINAGMYYKHRTKSKDPNTIEYDNKRQMNVTVFHQERFLPVF
                     SVMYFDTKMIDAAENYNYFFPDVAWNPNNAGFNVAGAAVTQANLITSNGYIHCLDHVL
                     KPLNTIYTELKNDSNFSQFLSLYKKKETFAYNAELSLEYGNGERLYEHLFSQPLPNIA
                     SEWPTNDYSKLTELSSVGYSIFAPTNAALNTFFDNYWAKGGYAKLDDVSRESVEKLLY
                     NCVYQGALKSGGNSYDGNLAFPEEIRRGDIINSYGAVINVPLDAVPQKYRILCENGLL
                     YGCEELAVPTMFAAVTGPAYQYKDFTHYLIMLTSIGDIQNTLWSDETQLLSLMPDNAQ
                     IEAMGITYDSRNNYLVRGGKALANDFKISTTYAHLVDLSASTCGDTELDATSPGKQQV
                     FRAFSPDYIFYWYVMDGKLTNSFRFNERIYPQLKSDREVFVSFSELPSYKEDGSWSNG
                     KSYTYEGSMFVGMPSETTYKSFQQMMQNNKVDSLLPYHGFVNLMTIAGMYDGAKFNFV
                     QEGENHLMLIPVNSAVFDAIENSAIPGLSFDSAAYGAGSNIFDCCSVADDSSRYVLQM
                     YLKDYFVPLATAGISNYPYLGWKEDTSKGMESLNVIEIYDESTSRVTYQSVKIVITDK
                     GDKLTVRLLESDEEIEVSGDYHYLPFVFEDGCVHFINGMF"
     gene            16286..19543
                     /locus_tag="PLI37_01310"
     CDS             16286..19543
                     /locus_tag="PLI37_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOQ57690.1"
                     /translation="MKKIITLLILCLLLIGTSSAQKTLTGRVYENFGGKEELAVGVNI
                     SFNNAQNRMVEGTVSNSNGEYSLRVPLGGGPYTVVFSYIGMKTQSIPYNGQSSLNVVL
                     EEDAKALDEVVVAAKQEQKNEMGITLREQTAATERILLSEIIEFTPVTSVEEALQGRL
                     AGVDILTGGDPGARNSIRIRGTATLNTSADPLIVINGVPYSTDIDDSFNFATASNEDY
                     AAMLNLSPYDIESIEVLKDAASTAIYGTSGASGVLLITTKTGSKGKPRFTLSSKFTAK
                     FEPESIPMLNGKEYVAFIEDAIWNTANAKGVSTSAALLELLYDTPEINFNKEWRYFNE
                     YNTNTDWLAAVVQDAYTTDNNFSMSGGGEKATYRFSLSYADEDGTTIGTNMNRFSSTL
                     NIGYRFNDKLRVETDFSYAETDKKANWTDNVRSEAMLKMPNKSPYWIDDITLTPTDRY
                     FNRQNAEEFQGAFTGSKNFHPIAMANESFNNSLQQEEKMNFRLNYDILPGLIYTGYVS
                     MKFKTIKNRAFLPQSATGVTMDNTYANRSSDAYSDNLALQTENKLIFRRNWNGIHNIV
                     ATALWRTSDSQSSNYSNTIYGVASKHASDPSTNGAIVERGSGDSQVRSISGIGGINYT
                     FLNRYILSGTVNYEGKSSLGKANRWGLFPSLGLAWHIKEENFLQNADWLSSMKLRCSY
                     GQSGAAPSGTAPYMGTYSALGDGYRDNPSIVPVSVQLNKLKWQTASEYDAGFDAGFLD
                     QRLTATFDIYYKYITDLLQRRIAIPTTSGYNSQGNSIAYYNSGEMSNTGWEFRIDYTV
                     VQNDDWRITLNYNIARNINRIEKLPENLPESSYSLRNGTYAQRIIVGAPVGSFFGYRY
                     LGVYDTTLETYARDARGAVMTDLDGKPIVMKNGTYTCFPGDAKYQDINHDGVINQSDI
                     VYIGNANPVVTGGGGFTAKYKQLTLTTFLHYRLGQKIVNQARMNSEAMYGTDNQSLSV
                     LRRWRSPGDDTDIPRALWQYGYNYLGSDRFVEDCSFLRLKTISLSYGLPKNFCRKIKV
                     NTINAFVTAYDLLTWTNYTGQDPEVTLPSRVTDIAMDRAQTPPGKRISGGLIINF"
     gene            19557..21098
                     /locus_tag="PLI37_01315"
     CDS             19557..21098
                     /locus_tag="PLI37_01315"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ57691.1"
                     /translation="MKKIKHLVAVVLVMALAFTHSACSDWLEVYPQNNQVSDYYWSSK
                     EEVEAMLNGCYHYLREMVTSQLIPLGELRAGQIFSRSGNTNLQEFRVKETDNISNWGP
                     FYEVINVANGVISNAEKVLSRDDTYDKNRLNAHKAEAYFLRALSYFYLVRNWKDVPLV
                     LFPYEDDSQDYHMAQSDEAEVIAQIKKDLLTALESGAAKEYYVKAWENKGRATKWAIY
                     ALLADVCLWSESYADAEKYCNLILNSNSAYAPSFLSTPSHSSWFSIFNPGNSNESILE
                     LQWSEEDEQLNDLPYLFDPSSSRRVYAYSKKMTELFVEEYTHTQQRMAEAVRTMYGGF
                     YCNPPAAYLAAETGFVWKYFGSEVLTEKRTPEHYDPNFILYRTTEVILMKAEALIMQG
                     PERYSEALELIDQVRRRSNLSSTEIDPLNTTELELLECLLNERATELAGEAKAWYDML
                     RFGRRNNYQYKETFITARLLEFNKQASTSWFRSVLLDNNALFLPVWEQELENNPLLVQ
                     NSYYE"
     gene            21120..22943
                     /locus_tag="PLI37_01320"
     CDS             21120..22943
                     /locus_tag="PLI37_01320"
                     /inference="COORDINATES: protein motif:HMM:NF028443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5108 domain-containing protein"
                     /protein_id="HOQ57692.1"
                     /translation="MKKIHSKHTVLPIRLPALVLTVVALIFASGGCRDPYMGQMFELD
                     EGDETKITNIAFMEKYPEDYSLFLDFLKAADYYNALNDASSTVTVFAPDNAAMDSFMK
                     IKDISSFDQLDSAYARQVIQVHMLRGSLNEASFIQYLNEGSIGIPTLFGDYLTLSYGF
                     TNRDIDDVDLANSKPEDTLSIYINNMASVRDIAHQTVNGMVYKLGGVIRPLIETVVEK
                     MHDYGEYELFLEAIEQAGMTGLLNITSDTVQQAMGGYTVNPVNYTVLAVSDETFRSAG
                     IQTMDDLENYLAEKDPAGRSGIARSDSNSVIRRYVSYHILAGANTKSSLTYSLTPNET
                     KVFDTFQAHEIITIQTLVGQTFINRGSRDVCGFVRSDIKASNGYIHKIDGILPVWCPE
                     PSVVVWDFCNSTDIVSIANSYGAANNLGNLYSTPSDAAEYKVDITKSNPYGTIHSFDY
                     KLASTKGTWLRIGYWKCKANTDATLDYENEFNAHLNNLLILNLGYTGWIEFTTPTIVK
                     GTYRVELYYAGAKALAKKFYGGGSAVKFTLDDFLKQHYLWKGWESSQQAVQGEVLFPN
                     ITFETTQSHSFKAVFMDANASTISTYRQMWDYVKFIPLAEE"
     gene            22971..24782
                     /locus_tag="PLI37_01325"
     CDS             22971..24782
                     /locus_tag="PLI37_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57693.1"
                     /translation="MKKHQSILILSLLSLTLAFTACGDAWDNHYGKQTPVADGNVEIY
                     PGSLKEFLASAPEFSTQHALFADAGIYDKMLPGQEYTILLYPDNILDTTSYAQDNSYA
                     AYCICDIALSPADMEEGMGISTWYGKNLWISLQGEQIKINDCPVEAITRTNNAFVYRI
                     NEKVIPVRLSVYDVINGLDDARYSKFKSLIRTYERRYFDPAKCTPAGTNEQGNTIYSD
                     SVKGWSTKNILMDRYSKDGQEMWNMRNEYYNSTVFIPDNDIIQAAVDTALARVPLWLG
                     RTATAADRNKFEKWIIRACFVDRLLNAGQVTGSEDIDCVGGYQKDSLEGKTVFEADMA
                     MWRPWVQKVRTGDMISASNGNIFFVDWLKIPNNVVIYRLKSRFYELWNNMNQEQRDKY
                     FRWDHWIDPMVLNDAQSSFTLSETMPTMYYHVLTAIPDKDARRDSLVCSVTYDGVLFN
                     QNNPRGSQIRECYIPAGEYYLRMGFKHSLLYSLSIQFCDTLLVEDMVMYAQGSNFHFD
                     RGSVSVVDNYGESSIGYPEGYNWHDWSSLSEKAQAYDTDGYPVGIVNMKEDGNFTITI
                     SSSDMSYLYTYGADRNTHNISQLMMYHWCLRPTKNNY"
     gene            24838..27627
                     /locus_tag="PLI37_01330"
     CDS             24838..27627
                     /locus_tag="PLI37_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOQ57694.1"
                     /translation="MGQGNVIYTKDMEKSYDIDHAIKGKIPGLQVVGKSGMPGEGAYL
                     NSGGIHSLYAANTPLIVVNGVPYMYDTKLSTAINGYSRSLFAALNINDIRSITLLTGS
                     DAAKYGSLGSNGVLLIETEQATSDNLNTRISLCTQYGLHFKGRTVDVLDAGGYKNYLR
                     SVGLSRYGQMGPLTTDYPFLQTSSDYPSAYIFNNNTDWQSEIYRPALVTNNVFRVEGG
                     DEIAKYNMSVGYSSDGGILYGTRTDRYHTLINSDITVSRKIDIFSSVGLSYLRSQLQE
                     QGMNKETNPLLAAWFKMPVLNPYFAGSDGELTGIYAPYNFSNINDYPQYPYENISNPT
                     AIVNTLEAADKVYDVNILLGLNYQLTNKIKLTAQYNRQYRYTQEDLFIPGTNNQAIYP
                     QYYGIGRNTVRKAVAENRNSFYGLNVSYRNTPNRIHQWEIDAGGRIMSTSAEYDMSSG
                     YNTANDFYKTLDMTTDEEYSDGYISQWLWANYYLNTGYTWKEWLAANLNFSVDGSSVS
                     GIDAPRYYVYPAGALKFLAARLSFIPEWIDRLDLSAEYALTGNSRFSSNYAKNYYNSS
                     NFFAMGSIIRGDIPNTLLEPEKHNQFKAGIDMSLLNKRVQLSAAAFNSYAYDLIIPQN
                     ISSIYAADNYYENSAAVSTDGLILSVRFVPVETNKFSWTLGGNLTTNKSVVKDLGEQT
                     YMDIYYTQFSEGEDAIVRLEVGETPYQFYGYKTNGIYETSAEARADNYTSVYNSKYKA
                     GDIRFVDRHADGIINEQDKQLLGSTSPDCFGSLFTQLRFRQISLNAQFNYRIGGKIYN
                     AVRRQLESMDNFHNQSVAVLNRWQAEGQKTTMPRAVYGDPSGNNVFSDRWIEDGSYIK
                     LGSLSLSYSIDQPIGKLFRSGTVWISGENLLTFTNYLGNDPELAYNYDEALYGMDFAK
                     VANPITLKIGFNLNF"
     gene            27633..29414
                     /locus_tag="PLI37_01335"
     CDS             27633..29414
                     /locus_tag="PLI37_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57695.1"
                     /translation="MKKYLTILSLSAILGLTSCEAMFITDPDDIINVDDYISEDDEMY
                     KGFLGIMTRMQQSADHSIILTDTRGDYFEVTPNAPLELQKIYSYEPTDGNSYADPAPY
                     YAVVIACNDYIDKMQDYREKAGENMDNNSSKNFNALISSTIRLKVWTYLKLGSIYGEA
                     IWFDNPLEEKIDLSDHTVFSRCNMNQIVNRCLHLLDSGLVLYKNEVISSKIIMDWASW
                     INEEKVTDNYIHWQYMIPEYLPLRCELLLWRGQEEDFRWVRDNIMDYLFKVHMGTIND
                     GVNVAFNDFRYACNIPIRTGADNLYATEYYRMFYNELYNASNFTNFYQVITGIMYDYK
                     NHQTNRIIEYFCPRSPGKYYLRPSSYAVAKYPESDIRGIVQRNNMDVIDGDTCFCKYY
                     YHRGEWLRTRIVEIHPVIPLFRGHDYHFYLAEAENHLHQWHQAEVIMNAGVTNEFADK
                     ILPNYWNSNYSTWFCPAGGYGDIGVAGCVYGKMRDYPVRNLVKTDKTSDGTGPFYNSK
                     NEVFDEDARMREYDLLIMDEALAEYAGEGRAYAIVCRMAERYGAEAVVDRIADKYKGG
                     PYYDQVRNAILSGDYWVRWDLKADDLN"
     gene            29458..31263
                     /locus_tag="PLI37_01340"
     CDS             29458..31263
                     /locus_tag="PLI37_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOQ57696.1"
                     /translation="MKRLVVYTTFILLFCFCVRAELTVIRAIPPDGSTGVDINSRVVL
                     VFDKNISEGRGIACSLNGTVVVPAVSSKMATFILPCMDYSVSYQFHAPAGAFIEKDNP
                     SNLSPEIRLTFTAKSRPQPEARLFDAIVSADSTGDYISVQAAINAAPEKRSKPWLIFV
                     RNGVYNEVIRVPESKPYIHLIGENADSTIIRYKICCTFLVDGGYTGPLEEAEAFGAYA
                     AQFSAGYARGSLGIDAMAHIDAKNFYSENISYINLWGYEERNYPQALAVWCTKDRTAF
                     YNCKALSYQDTWRTPSSGGYRQYLKNCLIEGNVDFIYDNGDVFFDSCQINIIKNDKAT
                     EEAGWIVAPSHSGTKWGYVFKNCGLSAEKKGDSIYLGRAWIAQPKAVFINLQLDDKVV
                     LRPKGWDSHFGQAIPIIFADWGTRNADGSPVDLSQRNNWYWYLNGNDTVQGYSRQYLS
                     DEQAATYTFHNVIPGSDAWRPDLMAERLPAPHLKIESGLLSWNSVPFAICYEIRYRGK
                     FVCFTTENNYRPASAATDYQVRAVNEWGGRGLLSDYATGLKSTSPATATIVRTDFFDL
                     MGRMIPENNISTGKQLVLRKISYSDGNVVVVKEIR"
     gene            complement(31268..31966)
                     /locus_tag="PLI37_01345"
     CDS             complement(31268..31966)
                     /locus_tag="PLI37_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ57697.1"
                     /translation="MTEEKMHIMLCEDDGNLGSLLCEYLQAKGYIVDLLLDGEQGYKA
                     FQKTKYDLCVLDVMMPKKDGFTLAQDIRMMNADTPIVFLTAKNMKEDILTGFKLGADD
                     YLTKPFSMEELLFRIEAIIRRVKGKKAKEVTLYHIGKFTFDTPKQILSIGDKQTRLTT
                     KECELLTLLCSHQNQILERNYALMTIWGENTYFNARSMDVYITKLRKHLKDDPDIQIM
                     NIHGKGYKLVVPEE"
     gene            complement(31963..>32157)
                     /locus_tag="PLI37_01350"
     CDS             complement(31963..>32157)
                     /locus_tag="PLI37_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ57698.1"
                     /translation="YLKKVFERFYRAPSGNIHNVKGFGLGLAYVKKIISDHKGCITAE
                     SELNVGTSFIIHLPLIKKQL"
BASE COUNT         8968 a         8015 c         7172 g         8002 t
ORIGIN      
        1 gtgcgccttt tggcttcttc ggtagaaatt cctgccaggt agatggtttc tactattacc
       61 gaacaaaaat gcttgtaaaa atctttttct atgctgcgcc gttctttatc gcttttgtcc
      121 gggaaactgg cagccaaatt gttacggacc accttttttc ggtaccctgc caggtaataa
      181 accaggggat acaaaatcat atccgaaagc aaataaagca tgtttagcgg caagaaagcc
      241 agcaagcgac aaaatccgta taagaacaca tagcccatag caggtgatta tactataatc
      301 acaaaggtag tggatttttt taaggtaaaa gtttttcctg tcgggaaatc aagacagccc
      361 acatggaatc ggtacagcct gcagagccga gcgatctgca ttgaaatcac cgagagtcaa
      421 aggaatgctg cgattttccc aatcgctgcg ccaaggtgct tccacgcgta cgtagttttc
      481 ggtaaaacca tgaataaggt tgttcttttt ggctttttca aacaatacga ctgcctgctc
      541 gccgcggcgc tcaccataaa atttcagtct ttttgtttcg gatagactat gtagtatctc
      601 gctgcgcctt tttagctcaa agctactcac cttcggctgt atctttaaag cttgcgttcc
      661 gggccgttcc gagtagctga atacgtgaag ttggctaata tcgaggcttt cgagaaagct
      721 tctgctctct tcgaaacgtt cttgtgtttc gcccctcatg cccacgatta catccacacc
      781 aatgaaagcc ccgggcatac tttgacgaat ttcagctact ctggaagcaa aatgttccct
      841 gtcgtatcgc cggtgcatca gacgaagcat ctcattgctg cctgcctgca ggggcaaatg
      901 aaaatgaggc acaaagcggg cggaggcagc cacaaactca atgatctcgt cgctgagcag
      961 atcgggttcc agcgaagaaa tccggaaacg ttcaatgccc tcgactccat ccagagcccg
     1021 cagtaagtct atgagttttt cgccgctgct cttaccgaaa tctcctatgt ttacaccggt
     1081 caaaacgatt tcgctaccgc cttcggcagc cacttcgtgg gcctgccgga cggcttcttc
     1141 gatgccgata ttgcgacttc ggcctctggc aaaaggaatg gtgcagtagg tacaaaaata
     1201 atcgcaaccg tcctgcactt tcagaaaata acgggtgcgg tctcctcggg cgcaggccgg
     1261 aaaaaaacga tgaatccccg agttggggcc cgaaaacacc tcggcttttt ctaatttact
     1321 gctccgaagg tattgcagaa tatccatctt ttcatctgtg cccagcacca gatccacacc
     1381 cggaatagct gcgagttcgg ccgctctgag ctgagcatag caaccgatga ccacaacaaa
     1441 ggctttgggg tatttgcgaa tgagcctgtg aatattctgc cggcattttt gttcggccgt
     1501 tccggttacc gcacaggtgt ttataacgca gatatccggc tcccgtccga cttttaccgg
     1561 ctgataacct acttgttgca tttttttacc caagcccgcg ctttcggcaa aattcaattt
     1621 gcagcccagg gtataaaaca aataagtggg agatggtgtt gttacttgat tggtcataaa
     1681 caaacaggat aagcgggatt ggttttacac ttatgctatg aacctggggt atttcttcga
     1741 tattacaagc gacgaaagag atcgggatta agaggcgaag ttttcgagac agttacaaaa
     1801 cggaaacagc tgcagagcga gaacaattgc agataggaaa cagttgcata ataataaaaa
     1861 gagggtgtct caaaaaattg agacgccctc ctgtctgtat attttgcaga attactcagc
     1921 gactacctca aagctgattt ccacctgaac ttcgcggtgg agcttgacag tggctttgta
     1981 ttctccaact tccttaactg cctccttgag caaaatgctc ttgcggtcta cctccaggcc
     2041 ttgttgttcg agcgcctcgg ctatttgaat cgggcccacc gaaccaaaaa ttttgcctgt
     2101 gctgctcact ttggctccta tgctcaggtg caagccctcg aatttggaaa ccatatcctg
     2161 agcatccgct ttcatttttt ccaatttgtg ggcgcgttgc ttaaggtctt cggccagcac
     2221 ttttttggcc gactccgtag ccattacagc caaaccgttt ggaatgaggt aattacgtcc
     2281 atatccgtct tttaccttaa caatatcgtc tttgtagccg agattgatta catcttcttt
     2341 taaaataact tccatgtcga ttcctccttt tacttcatca agtcggttac gtaaggcaag
     2401 agcgccaggt ggcgggcacg ttttacagcc tgggctactt tgcgctgata tttcagcgaa
     2461 gtgccggtaa gacggcgggg caagatcttt ccctgttcgt tgagaaactt tttaaggaat
     2521 tcgggatcct tgtagtctat aaacttgata ccgctctttt tgaaacggca atatttcttt
     2581 ttcttggtgt ccaccgtggg aggaaccaga taacggattt ctgagctatt ctgtgccata
     2641 gtttaattct cctttttagc ttgtttcaaa cttcttcttt tttctgcgta ttgagcaaag
     2701 aatttatcct gcttcaatat caggaagcga aggactgatt cgtcacgccg gaaattgacc
     2761 tccaatttag ccaccacttc cggattggcc ttaaattcga acatctgata aaaaccggtg
     2821 gactttttat caatgggata ttccagtttg cgcagtcccc agttttcttc attcacaatc
     2881 tcagcaccct ctgcaacgag aaggtttttg aatttttcta ccgcttcctt catctgaggc
     2941 tcagacaaaa cgggagttaa aatgaaaacg gtttcgtaat tgttcataag aaaatttata
     3001 attgttttta tactaactct tttgctggaa tagtgttttc acaaaaagag cggcgcaaag
     3061 gtagtaagat tattttttct gacaaaatct gttttaaaaa aaatcaggat gtaaaaaaag
     3121 ccgaactata atcgatattt gaataaaaag tgtaactttg cgcacattag tagcaagaac
     3181 aattcattca attagattac gatgaaaaag atattaatcc ttttgctcaa gaacctcggc
     3241 attattattg ttctcgcagg cactgttgta cttgccgcca cccaattcaa gggcattctc
     3301 accaatacct ggcttttagt ggctgcaggc ctgtttgtgc ttggattaat cacacaaatc
     3361 ttcattaaca agagagtgga gtaaacacaa cactgataat gtgttgagga ggaaaaacgt
     3421 ttggcttttc ttcctttttt tgttggcagt cctcagacct taacatcgag ccggcagatg
     3481 tccggcaaaa tctatatccc atgaaagcaa caagctcttt gcggaagtca ctttttgcat
     3541 acataattgc tttctttttg accccggcag agttttcttt atcggccaat tcccgggtaa
     3601 aaccttttgt aacctacaat cactacagca gcgatgatga gtttttgcaa ggtagcatca
     3661 cttatttgtt gcaagatcgt cgcggattga tttgggtgag ttcgttcaac ggactaaaca
     3721 aatacgacgg ttatcaatgg accaaataca aatcgcgtcc cggcgaaaaa tctctgctga
     3781 ataccaaccg tatcaactat ttatgggaaa acagcagcgg taacttttgg tgcgaagcag
     3841 gcaaccaaat ttatctgttc gatgtcgaaa aagaagaatt taccaatctg caaacagtct
     3901 ttgaacaaaa atacctcaga aatgtaacta ttgagagcat ttttcccctc ggcaacggag
     3961 tgagctatat gatcagcgag gtgggagaat gttttcgaat tgtggattcc gatcctctga
     4021 acaagtgccg ggagatagca atcaaagctt cagcttcgaa ggacgttttt ctggtgtttt
     4081 tagatcctca gggttttgag tggattttta ccaaaggtgg cacttaccgt tacgacacgg
     4141 atcagcttgt aagcaaaata ccttttcgct ttcctgccgt aaccgaggac gaattatggc
     4201 ttacttctgc cgatggcaga caaatgggga tctattccta tcaaaacgcc aatattacct
     4261 atttggattt tatcccattc aacggttacg tctataaaca gcattttaaa gacagtttaa
     4321 tctatacggc taccggggca ggtttctatc tcacatcgat cgccgacagg caaacgcttt
     4381 gtctgagtaa agaaatactg caaacttttt ttgtcgacag ccggggctgc gtctggggcc
     4441 ttactaccga taaagacatt gtcgtaataa acacaaaacc cgattccggt gaaacagttg
     4501 aatgccaaat agtagaactt ccgacggacc atcacttttc cgatccccgc gtgagttttt
     4561 acgaagacgg gagcggacgc atatgggcta ttttctacga cacaaacgac attttgtatt
     4621 acaacgaagg taccgggcga tttgaaaggc ccatcagccc ggaagggatg ataactaata
     4681 cgcaaggagt cgctatagat aaacaaggca atctgtggta ccgaaccaac aggcacatag
     4741 atatgcttac tttacacaac cagcctttcg aagtaatcaa ctcttttccc ggagaagaag
     4801 tgcgctccat gctcaaagat tccaaggacc gctactggtt cggtctgaga gaaaacaggg
     4861 tgctttgctt tcggaagaat cgacttgtac atgagattcc aatcgagggc actgcgtatt
     4921 gcctttatga agattcatat caacgtattt ggataggcag caaaccgggc ggtttgtact
     4981 gtctggaatt taccgagggt tcggacaatt acaggcttag ccgctaccaa cacgacgaca
     5041 acgaccccaa gagcctgagt cacaacgatg tgtacagtat tttggaagac agccggggac
     5101 atctgtggat aggaactttt ggaggaggta tcaatctgtt caaacaaggt attttccatc
     5161 atcaaaacaa tcatctgggc tacgacagtg aattgcccga agccgtccgt ttcatttgcg
     5221 aaatcaggcc gggacttatg ttgatagggg ccagaaacgg attgttctcg ttcaacaatt
     5281 tgtttgatca cccggaaaac attcgcatgt ttcgcaatac aagacggtct ctggatatca
     5341 acagcctgcc cgacaacgat gtcatgagca ttttggttac aagggacagc tccatttatc
     5401 tgaccaccag cagcggagga atatgccgta tactctccac aaccgatgat ctgctctcgg
     5461 acaggatccg ttttgaatcg ataaccaaag aagacggcct tgccagcgat gtcgcctact
     5521 caataataga agattcccaa aacgacttat ggattaccgg cggccgggca ctttcgcgac
     5581 tgcaacgcca cgaagtttcc gataattccg gcctgcaaag ccccggaatt attactattt
     5641 acgacaggac ttcctttcct tccgaggtga tattttccga aggccatccc cttacagtgg
     5701 gaaacattat gattttcggt accatgaacg gtatcgttac catacgttct tcgcaggttt
     5761 taaaagacat ttatactccc cctttgattg tcagcagcaa atcaaaagac cttaaatcca
     5821 agcatcaggc cagagcggcg agcatacgtt ttgccgccct ggattaccac gataaactac
     5881 acctcaaata cgcatacaag ttggacgggg tggacaaaga ctggaatatc acaaccgaga
     5941 ataccgttac ttacatcaat ttgccgcccg gcaacagttt attccacgta aaatcgacca
     6001 atgccgacgg tatttggacc gacaatgaac aaactatgct catttacgtt aggccgcttt
     6061 ttcatgagac gctgccgggc cgtcttatcc taatttcggc cgtcttacta tttattgcac
     6121 tggttgttta ctacttccag cgtatgtacc ggctgcgcca tcgcttgatt cttgaacagg
     6181 aaagcaccga taccaaactc cgttttttta cagacatcag ccacgaactg agaactccgc
     6241 tcaccctgat agacggcccc gtggcagaag ttttggagga taagcagtta tccgaacaat
     6301 cgcgttacta tctcgaagta gtccagaaga atgtacgcag attgctgaac ctggtgaatc
     6361 aaatactgga tttccgcaaa cttcagcaca acaaaataac tttattgatc gagccattgg
     6421 atgtaaaagt cctcttgtcc cgtatcatgg aaaatttcgc cgaattggcc aatcaccatc
     6481 gcatcctttt caatttaaat tgtccaaacg acttgccggt gatatgggca gacagggata
     6541 aaatagaaaa aatatttttc aatcttctgt cgaatgcttt caaatattcc aacgacggag
     6601 ctgcaataag cataaatgtt tcgaagctga acaacagctt taatggggat gttgaacctt
     6661 cggtagtgat atcggtcacc gaccagggca agggcatcaa acgggaagac gtggagcaat
     6721 tgttcagccg tttcgaaacc gtaatacaag acaatttgtt caggccctcg tcgggcatcg
     6781 gactatcgct ggtaaagcaa ttcgtcgacc tccatcacgc cagcatcgtt gtagatagcc
     6841 agccgggaaa aggcagtacc ttcagcgtaa ctttccgcgg aggaaaaaag catttcgaaa
     6901 acgacaagaa tgttgaattt tacgccggca accttagtga aaacattcat caggaacaac
     6961 cggcagaatc gcatcaggag gacaaaaccg aagaagaacg ctccaaaatc cttgtggtag
     7021 aagacaacga ggaattgcgc cattttatct gccgtatcct tgccgccgat tatcgggtag
     7081 tatgcgccaa cgacggcgag gaaggtttgc gcatgggcaa aatggaatgg cccgatttaa
     7141 tcattaccga catcatgatg cccaaaatgg acggttttga aatgattcgg cacatcaagg
     7201 ccgatgccga tatttatgcg gtccccctga ttgtcctgac ggccaaaggc gaaatggacg
     7261 accgcatata tggagtggag atgggagtgg atgattacgt acttaaaccc ttcagcgcct
     7321 cctatttaaa agcacgtgtc aaggccttgc ttgaacaaag acgccaatta caaaagcatt
     7381 ttctggaaat actgagccag ggaggcaatg cgctggctcg ccgtgccatt gaacctaata
     7441 tgccggtcat tactccggcc gacgaactct ttattcaaga agccctggcc tttatggaga
     7501 aaaacatgga taatgcggaa ctcaccatcg atcagtttgc cgacgccttg aatatgggcc
     7561 gcaccgtctt ttacaacaaa ctgaagacca cgctggggct tacgcccata gattttgtgc
     7621 aggaaatgcg cattaaaagg gcagttcaat tgatgaaatc aggcgatttt accattgcgg
     7681 aaatagccta tcagaccggc ttcaacgatc ccaaatactt cagccgcagt ttcaaaaaac
     7741 acctcggatc aagtccttcg gaatacatca gatccatccg gaaataacca aaggagggcg
     7801 ccttaataat aagacgccct cctcacactc cattctttaa accattcacc taaccgcttt
     7861 caacacgcag attcctgtgt ttgcaataac cttagcaata aaaaaacgaa cggaacccaa
     7921 atcaagtctt acattttctt ctatttcgta ttggccggct ttactgccgt cgatagcata
     7981 caattcgacc cttgcttttc cgcaaaagcc gcttgccaaa agcctgctac cgtcgaaaac
     8041 gacattcagc gcgtcggccg tttcagtctg aatgccgttt acaaaagggg ggtctgaaac
     8101 ctgtccgagt gttccgtaca tattaacggc acgtacacga tagccggaaa catctccctc
     8161 aaaagtcagg gatgtttcgg ttgtgaatgt cagaaaatcc ccattgtaca gaacttcgta
     8221 acagatggcc tgaggctccg ctgtccagta aagacggttt tcgttgtaat acaaaagcgg
     8281 tgcaggcagc tctgtagtaa tattgtccgg cttccagccg tccgttccgc tcagcaccgt
     8341 acgaaccgtc agagcatcgg cttcttcctt gctcagtaca gcctgggcaa tacaggtttc
     8401 cgtgggatta ttcctgtcgc tgcctgtcca gtaataggta ttgcgcgaag ccagacttac
     8461 gggattaccc atatgatcta tggtattgta ttccgcaaaa acgaccggta agcctcccat
     8521 atgatcaatc caaccggccg gagacaggtt gatgtctttc gacaagcttg tattgataaa
     8581 agaagtccgc ggcctgttgt gccaggggcg gccccaataa ttatttgtac gagccgtacc
     8641 cggcttgctc gaaaccacgt tgcaatcacg aaaaacataa ccgtatttca ctcccccgcc
     8701 atgcgaaggg gcaacaacaa ccgaaccgtt acgacaaaga gcaatggtac aactgtcgaa
     8761 aacacaattg cccgaagcat aaataaagtc gacagctcct tcaatgtagc tgcgaaaagc
     8821 gtaatgacgg tgttcgtcac gacccgtctg ccaggtatcc tgataactct ttaaaacggc
     8881 attgaacatg gtaaagcgat cggcataact tcccaaagcc agcgcctgag ggcctgcctg
     8941 attgtcgtaa ccccaggaat tgattacggt aatattatgc aaataaaaat cggaagaatt
     9001 ggccaccaac accggcccct ggccttctgc ctcggggaat aatgaattat tgcgttcatg
     9061 tccgccatag gaggtgatgg agaacttcaa caccgtcttc tccttatctt ctcccaccag
     9121 acaaatattc ggctgacttg tggaagtgcg taccaactct tcgtaaaccc cgtttctgac
     9181 aaaaatcagc cagggttccg tacggttgtt aggggctgct tttaccgcct gacgaatact
     9241 ggtataatct ccggatccgt ctttggccac cactgcatcg aatacttttg ctttggggct
     9301 gcaggtggtg aaagaatagc taccgcccgc caagggcctg ttgtcaactt tagacaaaaa
     9361 ggccccgtcg cggacaagca ttgtataggt agtggcaaac tgtaactgtc ccggcttgaa
     9421 aatggccatt ccgcctgcta tgctggccgg agcaatgtat tcaccgttca ggaaacaact
     9481 gtcaggcccc tgaatcaacc caacggtact ttgaagtatt atttgggcat tcacgggtac
     9541 ctctttctgt ccgtccaccg gaattttagg aaaaacctcc ccggcggcat atacattgcc
     9601 ggcaagtaat aaagccgata ttatcgcaac cgggaagcaa acaagagccg ttttgatcac
     9661 cctgtttatt tttctattca acatcattgg tttccgtttt cggttcaaca aacaaaagaa
     9721 tcctcctctt tatagggatg ctgatttttt tgaatttaat tatttaatca ggtatttctt
     9781 tccgttgata atataaatac cgggacgatc ggcgctctta aggcgacgtc ccatcaggtc
     9841 gtagatcata tcttcctgct cttcccttac cggagccact acagcattag ggtcgtaatc
     9901 ggccaaaagg aagatattag ccacatatac accataaaca tcgaagatac ggttggtatc
     9961 gtatccactg gtgacatcgg gaatcaaacg gatatagatg agttccttgg ctgccgtatt
    10021 gggaagctct acctcgaaat cctgccagcc gccggtggta gtaaccgtgt cgacggtttc
    10081 ccacgaagta ttatcgtacg aatattgaac aaaataatcg taatgtccca tataagtacg
    10141 tctgatggag aagcaatatt taatgtcgct catgtagccc ctggtacaaa cagccgtttg
    10201 ccaatagaaa ccctggtctg tataatactt gcttgagtcc atggcgccat cactggtttt
    10261 atattcggtg ccatcgccgg aatcggtcag acgcagccag gtgacggcag cggtataagt
    10321 atcggtacga ttccaccaag tcttagtcca gggagcatcg ggatacaggg tataatacat
    10381 atccaaagtg gggacatgag ctgtatcctg gtaattacga ctcaggaagt cggccggacg
    10441 ttgttggttt actgcgcttc gttgtgtatc aaagtcccac ccgcaaatga aatctttctg
    10501 aacaaattcc agtacaatcg tataatcttt acccataacg atggagaatt ccggtgcata
    10561 aaattgctcc gacatatcgt ctgtatatcc cattacaaat tcggtaatca aattggtctt
    10621 agaagtaata accaattcgg tgccttcttc gtaatattca taagctccgt ctttacctgt
    10681 ttcggaaatc acataagaac ctcctccctt gcaggtaatg tttagtctaa acaaaggtat
    10741 ggcatcaaaa acggcatata catatttatc gccggtcatt tcgaaagaat agctggtttc
    10801 ttcgctcagg gtggcattgt tttcatccac ccatttcaca aacttgaaac cgtagttttt
    10861 gacagccgtc aaagttacgt tttcgccttc gggtattttt ccactagggc cgctaatggt
    10921 tccggccaga gagtcaacgc cggcagccag accgtgagtg agattgtaaa gttgaccggc
    10981 atctttcagc catttctgag ctcccagtac gccacccgtg gtcgatttgt tctcaaccgg
    11041 agtcacacct ttatataaag agaaattacc gttggccgga tcggcaaaca aagcagcagc
    11101 ggaaggaatt tccaggtcgg ctaaactata gttgttttcc atagaaacat ctccaagttc
    11161 ttctgccaaa gtaggaattt cccatccgtg aattaaatta ttcttgatgg tgacgtccca
    11221 ataaccggca gaaatagaga acaatttacc tccgaccttg ccatcagggc aaataatgat
    11281 attgtcggta aaaaccagtt ttgaatcttc accgttaaat ttattggtca cctggcagaa
    11341 ataacgatta gcatcacgac aaccctgata aagagtgttg tgtgtgaaaa taaattcgtg
    11401 gctcttgccg tcgtaggttc ctctgggatt gtagaatgat tccactccgt cgttcttgta
    11461 aaaggtacat tcattcatga tccacttgta aatgggagtg gtatgccaag aaaagttcca
    11521 gttgcccgtg ttgttattat agaatgtaca accctcaaat tcaactaatt ccacagctga
    11581 agaagcttga tctccaccca gatagaaagt acaacgattg atattctgaa tcatacaatt
    11641 ctttaaggaa atagccttaa tagtacccac gggagcaaaa ttgaacagat agctggcgcc
    11701 gtcgccgatg gtaatatttt cgagatagag ccccgaaccg tcgccaaccc cctcacccct
    11761 gatttctccg gtcaggaaaa cttcgcaggt gtcgggagct aaaatggtga tagctttgcc
    11821 aacagggaaa ttttgattgg cataagtgcc actgtacaat agcagtgtgt cgccgtcggc
    11881 cgttgccccg tatttggtag cataattgga cacgctgtcc acgacaatta cattggctga
    11941 agccacaaaa gcaagcagca atgaacaaaa caaaagagta aattttttca tagtcggtta
    12001 atttaatagg tttagtagtc aattaccacc aaaaataaag catcgaaatt ttctcttagt
    12061 ggattatttg ttgagatagg tgtacttttt aaacaaaaaa tatcctccta ttccgcaaaa
    12121 aagtacaccc aaaggccgct taagcaaggt atttttgttt cgatatataa taaacccgat
    12181 accgatgaac aaatatttat tgctgatttt gcatttatgc ttctgcgcag cgaatttttc
    12241 gttgatggcc gacactattc ccgcttttcc cggggccgaa ggccacggac gttttaccac
    12301 cggcggacgc gggggccttg tgtaccatgt taccagctta gaagacgatc ctgtaaatcc
    12361 caagttcggc accttgcgct attttctgaa taaaaccgaa aaacgaacca tagtttttga
    12421 tgtgtccggc accatctttt tgaaagatgc cctcaatatc aatgcaggca acttaaccat
    12481 tgccggacag acagcaccgg gtgacggtat ttgcctggcc ggttatccgg ttcacgtcaa
    12541 ttcgagtaac attattattc gttttctgcg tttccgcatg ggcgacctcg atcccaacaa
    12601 cgaagacgat gctttgggag gaagaggcat caaaaacatc gtgatagacc actgctcctg
    12661 ctcctggagc gtagatgaat gtgtttcttt ctacgggaac gagaatctca cgctgcaatg
    12721 gtgtattatc agtgaaagcc tgcgtcattc caatcacgaa aagggcaatc acggttacgg
    12781 aggcatctgg ggaggagcca acacttcgtt tcatcacaac ctgcttgccc atcacagcag
    12841 ccgtacaccc cggctgggcg cggcagacaa tacggacggg cgcgacaacg tagaccttag
    12901 aaataacgtt atttacaatt acggtctggt agtaggctgt tacggagccg aaggcatgga
    12961 cgccaacatc attaattgtt attacaaacc ggggccttcc accaaaacca accacaaccg
    13021 ccgtagtatg ataatcggca tcggagccaa caccaccgac agtgcccggc cgaaatgggg
    13081 aaaatactac atcgccggca attggtgttc ttccctgagc gacacttata aattacccac
    13141 cgacagctac gagcgcaata cttgtctgga caattggaat tacggagtgt acaacaatat
    13201 atcggaacct atcagtgccc aggaaaaagc ggcgatgaga ctgagcgaac ccaacaatcc
    13261 gggcctggta acgactcata ccgctcaggt cgctttcgaa aaagtattgg gcactgcagg
    13321 ctgttcctta caacgggatg aaattgacct gcgtattgtg gatgaaaccc gccggggtac
    13381 cgctactttt aagggcagtt acactgcagt gggcggtatc atcgacagcc agaacgacct
    13441 taaaccggcc ggtgccggca gcgactggag cccctggccc accttgaaca gcacggaacc
    13501 ccccaaagac acagatcggg atggtatgcc cgacgcctgg gaagaagcac ggggacttaa
    13561 tcccagatat cctctcgatc gcaacgacat tgccgaaaac ggctacacca acttagaaaa
    13621 ctacctaaat agtttagtcg aagaaatcgt ccgccaggaa ctttcagact acgaattcgt
    13681 taccggttcc ggccaaaatg atctgcccgc agccataaat cctcccatag tcgcaaaaca
    13741 aaaaaaagag aacaataaga tttggtacga cctgagcggc cgaccaatcg agtcgccaaa
    13801 cgcaagagga ttgtatatac gccgtggaga aaaattagtc attcgatagc caacaaaaca
    13861 ccaataaaac catgaaaatc agagcattcc gtagaaaaag acacgcatgg ctgccggcgg
    13921 cattcatcgc cgcgattgtg agcagttgca atgaccacat gtcggaatat tatgctcgtc
    13981 ccgactggat caagggaaac tgctacgaag tcctcgaaat ggatggcaat tataccattt
    14041 ttctgaaagg aatcgacaag gcaggttttc acgatatggt agacggcaaa agcatagtaa
    14101 cggtaatggc accgaacgat tctgccttta gcctctattt ggctgaacag ggactgaaca
    14161 gcatagagga aatggattcc tccgagctgg ataaacttat tggtttccac ctgatgtact
    14221 atgcctacag tgcagataag ctcatcaact ttcgtcccga agaaggcgac gatgtcacgg
    14281 atgaagagaa aaaaatcaac gccggcatgt actacaagca tcgaactaaa agcaaggatc
    14341 ccaacaccat cgagtacgac aacaaacgcc agatgaatgt taccgttttt catcaagagc
    14401 gttttttgcc tgtgttctcg gtcatgtatt tcgacaccaa gatgatcgat gcggcagaaa
    14461 actataacta tttttttccc gatgtggcat ggaatcccaa caatgccgga tttaacgtgg
    14521 cgggcgcagc agtcacacag gctaatctga ttacgagcaa cggctacatt cattgcctgg
    14581 atcatgtgct taaacccttg aataccatat ataccgaatt gaaaaacgac agcaatttca
    14641 gtcagtttct aagcttgtac aaaaaaaagg agaccttcgc ctacaatgcg gaattatctc
    14701 tggaatacgg aaacggagag cgtctgtacg aacacctgtt ctcccaaccc ttgccgaata
    14761 ttgccagtga atggccaacg aacgattact caaaattgac ggaactctcc tcggttggtt
    14821 acagtatttt tgcacctacc aacgctgctc tgaatacatt cttcgacaat tactgggcca
    14881 aaggcggcta tgcaaagctg gacgatgtca gccgtgaatc ggtcgagaaa ctgctttaca
    14941 attgcgttta tcagggagcc ctcaaaagcg gcggcaactc ttacgacggc aatttagcct
    15001 tccccgaaga gattcgccgc ggagacatca tcaatagtta cggtgccgtt atcaatgtgc
    15061 ctctcgacgc tgttccccaa aagtaccgca tactctgtga aaacggactg ctctacggct
    15121 gcgaggaatt agctgtcccc accatgttcg ccgccgtcac cggcccggcc tatcaataca
    15181 aggatttcac tcattacctt atcatgctca cttccatagg tgacatccaa aacacccttt
    15241 ggagcgacga gactcaattg ctctcgctga tgcccgacaa tgcccagata gaggccatgg
    15301 gcataaccta cgacagccgc aacaattacc tggttcgcgg tggtaaagcc ttagctaatg
    15361 actttaaaat cagtacaacc tatgcccacc tggttgatct ttcggcgagc acctgcggcg
    15421 atacggaact cgacgcaaca agcccgggca aacaacaagt gttcagggct ttttcgcccg
    15481 attacatttt ctactggtat gtgatggacg gtaaactgac caatagtttt cgtttcaacg
    15541 agcgcatcta tccacaactt aaatcagacc gggaggtgtt tgtttctttc agcgaattgc
    15601 catcctacaa agaggacggc agctggagca acggcaaatc ctatacctac gaaggatcca
    15661 tgtttgtcgg tatgccttcg gagaccacct acaaatcgtt ccagcaaatg atgcagaaca
    15721 acaaagtaga ctccctgctc ccctaccatg gttttgtaaa cttaatgact atagccggca
    15781 tgtacgacgg ggcaaagttt aatttcgttc aggagggaga aaaccattta atgcttattc
    15841 ccgttaactc tgccgtgttc gacgccatcg aaaactcggc catccccgga ttgagttttg
    15901 attcggctgc atacggcgcg ggaagcaata ttttcgactg ttgctcggtg gccgacgaca
    15961 gttcaagata tgtactgcaa atgtatttga aagactactt cgttccgctt gccacggcgg
    16021 gcatcagcaa ttatccctat ctcggctgga aagaagacac ctccaaaggc atggaatcac
    16081 tgaacgttat tgaaatttac gatgaaagca ccagccgggt cacctaccag tcggtaaaaa
    16141 ttgtgattac ggacaaggga gataaactca ccgtccgtct gcttgaaagc gacgaagaaa
    16201 tcgaggtatc gggagattat cactatctgc cctttgtgtt tgaagatggt tgtgttcatt
    16261 ttattaacgg catgttctga atagtatgaa aaaaataatc accctattga ttttatgctt
    16321 gctgctgatc ggcacaagct cagctcaaaa gacgctgacg ggacgtgtct acgaaaactt
    16381 tggaggtaaa gaagagcttg ccgtaggtgt aaacatctcc ttcaacaacg ctcaaaaccg
    16441 tatggtcgaa ggaacagtct ccaacagcaa cggtgaatac agtctgcgtg tcccgttggg
    16501 aggagggccc tataccgtgg tcttctccta catcggaatg aaaacccaga gtattcccta
    16561 taacggtcaa tcttcgttga atgtagtact cgaggaagat gccaaagcac tggacgaggt
    16621 tgtggtagca gccaagcagg aacaaaaaaa tgagatgggc atcaccctga gagaacaaac
    16681 ggccgctacc gaacgtatac tactaagcga aattatagaa tttactcctg tcacttcggt
    16741 cgaagaagca ttacagggcc gtctggccgg tgtggatatt ttgaccggag gcgatccggg
    16801 agccaggaat tccattcgca ttcgcggtac agccacactc aataccagtg cagacccttt
    16861 gattgtgatc aacggtgtcc cctattcgac cgacattgac gattccttca attttgctac
    16921 ggccagcaac gaagactatg ctgccatgct gaacctgtcg ccttacgaca tcgaaagcat
    16981 agaagtattg aaagacgccg cctccacggc catttacggc acatcgggcg ccagcggtgt
    17041 actgctgata acgaccaaaa caggatcgaa aggcaaaccg cgtttcactc tgtcttcgaa
    17101 gttcacggcc aaattcgagc cggaatcgat tccgatgctc aacggcaagg aatacgtggc
    17161 gtttatcgaa gatgccatct ggaatacggc caatgccaaa ggcgtctcga ccagtgccgc
    17221 tctgctggag ctgctgtacg atacgccgga aattaacttt aacaaggaat ggcgctattt
    17281 caacgaatac aacaccaaca ccgactggct ggccgcagtg gtacaggatg cctatacaac
    17341 ggacaacaac ttttcgatga gcggcggagg cgaaaaagcc acctatcgtt tcagtctttc
    17401 ttacgccgac gaggacggaa ctaccatagg caccaacatg aaccgttttt ccagtacact
    17461 caacatcggc taccgcttca acgacaaatt gagggtggaa accgactttt cgtatgccga
    17521 aacagacaag aaagcgaact ggaccgacaa tgtccgttcg gaagctatgt tgaaaatgcc
    17581 caacaaaagt ccctattgga tagacgatat cacgttgact cccaccgatc gttacttcaa
    17641 tcgccagaat gcggaagaat ttcagggagc ctttaccggg tccaaaaact tccatcccat
    17701 tgccatggcc aacgaaagtt tcaacaacag cctgcaacag gaagaaaaaa tgaactttcg
    17761 cttgaactac gatatcttac cgggacttat ctacacaggg tatgtttcga tgaagttcaa
    17821 aaccatcaaa aacagagcct ttttacccca gagtgccaca ggagtcacta tggacaatac
    17881 ttacgccaac cgcagcagcg atgcctacag cgacaacctg gcactacaga ccgaaaacaa
    17941 attgattttc cgccgcaact ggaacggcat tcacaacatt gtggctactg ccttgtggcg
    18001 caccagcgac agccaaagca gtaactactc caataccatt tacggcgtgg cttccaaaca
    18061 tgcttccgat ccatcgacca acggggccat agtagaaaga ggttccggcg actcacaagt
    18121 tcgctcgatc agcggcatcg gcggtataaa ttatactttt ctcaaccgtt acattctttc
    18181 gggaaccgtc aactacgaag gtaaatcgtc gctgggtaaa gccaaccgct ggggattatt
    18241 tccttccctg ggccttgcct ggcatatcaa agaggaaaat ttcctgcaaa acgccgattg
    18301 gctgagttcc atgaaattac gttgcagtta tggccagagc ggcgccgctc cttccggaac
    18361 ggctccttac atgggaacct attcggccct tggcgacggt taccgcgaca acccttccat
    18421 cgtacccgta tcggtgcaat tgaataagct aaaatggcaa acggccagtg agtacgacgc
    18481 cggttttgac gccggttttt tggatcaaag actcacagcc actttcgaca tatattacaa
    18541 atacatcacc gatttgcttc agagaaggat cgccattccc actaccagtg gttacaattc
    18601 ccagggcaac agcattgcct attacaattc gggagaaatg agcaataccg gctgggagtt
    18661 cagaatagat tacacggttg ttcaaaacga cgactggcgc atcacgctca attacaacat
    18721 agcccgcaac atcaaccgca tcgaaaagtt accggaaaac ctgcccgaaa gcagttacag
    18781 tttaagaaat ggtacctacg cacagcgtat catcgtaggt gcacccgtcg gtagcttctt
    18841 cggttaccgt tacctggggg tttacgacac cactttggaa acttatgccc gcgatgcccg
    18901 gggagccgtc atgacagacc tcgacggcaa acccatagta atgaaaaacg gtacctatac
    18961 ctgtttcccg ggagatgcca aataccagga catcaatcac gacggcgtta tcaatcaaag
    19021 cgatattgtg tacatcggca acgccaatcc ggtagtcacc ggcggcggag gattcactgc
    19081 caaatacaaa caactgacct taaccacctt tttacattat cgtttgggac aaaaaattgt
    19141 caatcaggca cgtatgaaca gtgaggccat gtacggcacc gacaatcaga gcctgtcggt
    19201 attaaggcgc tggcgcagtc cgggcgacga caccgatata ccgagagccc tctggcaata
    19261 cggctacaac tatctgggca gcgaccgttt tgttgaggat tgctcttttc tgaggttaaa
    19321 aaccatctct ctctcttacg gtttgccgaa aaatttctgc cggaaaatca aagtcaacac
    19381 catcaacgct tttgttacgg cttacgacct cctgacctgg accaactata ccggacagga
    19441 tccggaagtg actctgccct cgagggtaac cgatattgct atggacaggg ctcagacccc
    19501 tccaggcaag aggatttcgg gtggtttaat catcaatttt taaaggagga ctcattatga
    19561 aaaaaataaa acatctagtc gccgtcgtcc ttgtcatggc attggccttt acccattccg
    19621 cttgctccga ttggctcgaa gtttatccgc agaacaatca ggtaagcgac tattactggt
    19681 ccagcaaaga agaagtggaa gccatgctca atggttgtta ccactacctc cgcgaaatgg
    19741 ttacttccca actgattcct ctgggcgaac tcagggccgg tcaaatcttt agccgcagcg
    19801 gtaacaccaa cctgcaggaa tttcgtgtca aagaaacaga taacatcagc aattggggcc
    19861 ccttttacga ggtcataaac gtagccaacg gtgttatttc gaatgccgaa aaagtattga
    19921 gcagagacga cacttacgac aaaaacaggc tgaatgcaca caaagcagaa gcttatttcc
    19981 ttagggcttt gagttatttc tacctggtca gaaactggaa agacgtgccg ctggtgctgt
    20041 tcccttacga agacgactct caggattatc acatggcgca gtctgacgaa gccgaagtga
    20101 ttgctcaaat aaagaaagac ctgctgactg ctttggaatc gggagcagcc aaagaatatt
    20161 acgtcaaagc ctgggaaaac aagggccgtg ccaccaaatg ggcaatatat gctttgctgg
    20221 ccgacgtctg cctttggagc gaaagctatg cggatgccga aaagtattgc aacctgattt
    20281 taaattcaaa ctctgcctac gcccccagct tcctttcgac tccctcgcat tcctcctggt
    20341 tctccatttt taatcccggc aacagcaacg aatctatcct ggagctccaa tggagcgaag
    20401 aagacgaaca actcaacgat ctgccttatt tgttcgaccc ctccagttcc agacgcgtgt
    20461 acgcctattc caaaaaaatg actgagttgt ttgtggagga atatacccac acccaacaac
    20521 gtatggcaga ggccgtgcgc accatgtacg gcggattcta ttgcaatcct cccgctgctt
    20581 atctggccgc agaaacaggc tttgtctgga aatatttcgg ctccgaagtg ctgaccgaaa
    20641 aacgcactcc ggaacactat gatcccaatt ttatacttta ccgtacaacc gaagtgatat
    20701 taatgaaagc cgaagcgctg attatgcaag ggcctgagcg ttattcggaa gctctcgaat
    20761 taatagatca ggtccgcaga cgctccaacc tgagttctac ggaaattgat ccgctcaata
    20821 ccaccgaatt ggaattgctc gaatgtttgc ttaacgaacg tgctaccgaa ctggccggcg
    20881 aagccaaagc ctggtacgat atgctgcgtt ttggccgccg caacaactac caatacaaag
    20941 aaaccttcat cacggcccgt ttattggaat tcaacaaaca ggccagtacc agttggttcc
    21001 gctctgtgct cctcgacaac aatgcgctct ttctccccgt ttgggagcag gaattggaaa
    21061 acaatcctct tcttgtgcaa aattcctatt atgaataacg aattcaagga ctaaagttta
    21121 tgaaaaagat acattccaaa catacagtgt taccaatacg attgccggcg cttgttctta
    21181 ccgtcgttgc gctcattttc gcgtcgggag gctgccggga tccttatatg ggtcaaatgt
    21241 ttgaattgga cgaaggcgac gaaaccaaaa tcaccaacat tgcttttatg gaaaaatacc
    21301 ccgaagacta ttcactgttt ctggattttc tgaaagctgc cgattattac aacgccctta
    21361 acgacgccag ttcaacggtt accgtttttg ctcccgacaa tgccgccatg gattctttta
    21421 tgaaaataaa agacatcagc tctttcgatc aactcgattc ggcttatgca cgtcaggtga
    21481 ttcaggtcca catgctgcgg gggagcctca atgaagcttc ttttatccaa tacctaaacg
    21541 aaggaagcat cggcatacct acccttttcg gagattacct gaccttgagt tacggcttca
    21601 ccaaccggga catcgatgat gtagatctgg ccaacagcaa acccgaagac accttgtcga
    21661 tttacatcaa caatatggcc tctgtcaggg atatcgctca tcaaaccgtt aacggcatgg
    21721 tctataaact ggggggagta atccgtcccc ttatcgaaac ggtggtagaa aaaatgcacg
    21781 attacggaga atacgagctt tttctcgaag ccattgaaca agcaggtatg accggcttgc
    21841 tgaatattac cagcgatacg gtacagcaag cgatgggcgg atatacggtt aatcccgtca
    21901 actacaccgt actggccgtt tccgacgaga cgttcaggtc ggcaggcatc caaactatgg
    21961 atgatctgga aaattatctg gcagaaaaag accccgccgg tcggagcggt attgcccgaa
    22021 gcgacagcaa tagcgtgatc cgtcgctatg tttcctacca tattttggca ggggccaaca
    22081 ccaaaagttc actcacttac agtttaactc ccaacgaaac gaaggttttc gacaccttcc
    22141 aagctcacga aatcatcacc atccagaccc tggtcggcca aacgtttata aacagaggca
    22201 gccgggatgt ctgtggcttt gtccgttccg acatcaaagc gagcaacggt tatatccaca
    22261 aaatcgacgg tattttgcct gtttggtgtc cggaaccctc tgttgtagtc tgggattttt
    22321 gcaacagcac cgatatcgtt tccattgcca acagctacgg tgccgccaac aacctgggca
    22381 atctttacag cacacccagc gatgctgctg aatataaggt agatataact aaaagcaatc
    22441 cctacggaac catacattct ttcgattata agctagcctc caccaaagga acctggctca
    22501 gaataggtta ttggaaatgc aaagccaata cagatgctac actggattac gaaaacgaat
    22561 tcaacgctca tctgaacaat ttactcatac tcaatctggg ctataccggc tggatagaat
    22621 tcactacccc taccattgta aaaggtactt accgggtaga attgtactat gcgggagcca
    22681 aggcgctggc caaaaaattc tacgggggag gcagcgccgt caaattcacg ttggacgact
    22741 tcctgaaaca acattacttg tggaaaggct gggagtcgag tcaacaagcc gttcaagggg
    22801 aggttttatt tccaaatatc acttttgaaa ctacgcaaag ccacagcttc aaagctgtat
    22861 tcatggatgc caatgcttcc actatttcca cttaccgcca gatgtgggat tacgtaaaat
    22921 tcattccttt agccgaagag taagctatgc acgcaaaaag aacgacagtt atgaaaaaac
    22981 atcaatccat acttatcctc agtcttctaa gtctaacgct tgcgtttacc gcctgcggcg
    23041 atgcctggga taatcattac ggcaaacaaa cgccggttgc cgacggtaat gtggaaattt
    23101 atccgggcag cctgaaagaa tttctcgctt ccgctcctga attcagcact cagcatgctc
    23161 tttttgccga tgcgggcatt tacgacaaga tgcttcccgg acaagaatat accattttgc
    23221 tttacccgga caacatactg gacactacct cctatgccca agacaacagc tacgccgcct
    23281 actgtatttg cgacattgct ctgtctcctg ccgacatgga agaaggcatg ggcatcagta
    23341 cctggtacgg caaaaacctc tggattagcc tgcagggtga acaaataaaa atcaacgact
    23401 gcccggtcga agccattacc cgcaccaaca acgccttcgt ttaccggata aacgaaaagg
    23461 ttattcctgt ccgactgtcg gtctacgacg ttatcaacgg cctggacgat gccaggtatt
    23521 caaaatttaa gtcgctgata cgcacgtatg aacgcaggta ttttgatcca gccaaatgta
    23581 cgccggcagg caccaacgaa cagggtaata ccatctactc cgactccgtc aaaggctggt
    23641 cgacaaagaa cattctgatg gaccgctaca gtaaagacgg ccaggaaatg tggaacatgc
    23701 gcaacgagta ctacaacagc accgttttta tacccgacaa cgacatcatt caggcagccg
    23761 tagatactgc cttggcccgt gtgccgcttt ggctggggcg tacagccacc gcagccgacc
    23821 ggaacaaatt cgaaaaatgg ataatcagag cctgctttgt agatcgactg ctcaatgccg
    23881 gacaagtaac cggttccgaa gacatcgatt gtgtgggagg ctaccagaaa gacagtcttg
    23941 aaggtaaaac agtatttgag gcagatatgg ccatgtggcg tccctgggta caaaaagtcc
    24001 ggaccggcga catgatttcg gccagtaatg gaaatatctt ctttgtcgat tggctaaaaa
    24061 tccctaataa cgtggttatt taccgtctga aaagccgttt ctacgagcta tggaacaata
    24121 tgaaccagga acaacgcgac aagtatttcc gctgggatca ctggatcgac ccgatggttc
    24181 tgaacgatgc ccagagctcc tttacattga gcgagaccat gcctaccatg tactatcatg
    24241 tactgacggc tatacccgac aaggatgccc gccgcgattc gttggtctgc agcgttacct
    24301 acgacggcgt tttgtttaac cagaacaatc cacgcggcag ccagatacgg gaatgttata
    24361 tccctgccgg cgaatactac ctgcgtatgg gctttaaaca cagcttactt tattcactca
    24421 gtattcaatt ttgcgacacc ctgttggtcg aggacatggt tatgtatgcc cagggatcga
    24481 atttccactt cgaccgcgga tcggtgtcgg tagtcgacaa ttacggggaa tcgtccatcg
    24541 gttatcccga gggctacaac tggcacgatt ggtcgtcgct gagcgaaaaa gctcaggcct
    24601 acgataccga cggttatccc gtcggcatag tcaacatgaa agaagacggc aattttacga
    24661 ttaccatctc atcttccgac atgagttacc tctatactta cggagccgac cggaataccc
    24721 acaatataag tcagctgatg atgtatcact ggtgcctacg tcccacgaaa aacaactatt
    24781 gatatgaaaa gagttctttc aattagcgtc gtactagtct tgtgcctttc gaccgtaatg
    24841 ggacagggca atgttatata cacaaaggac atggaaaagt cttacgacat tgaccatgcc
    24901 attaagggaa aaattccggg tctgcaagta gtcggcaaaa gcggtatgcc cggcgaaggt
    24961 gcctatttaa attcaggagg tatccattct ctgtatgctg ccaacacccc cttgattgta
    25021 gtaaatggtg ttccatacat gtacgacacc aaactttcaa ccgccatcaa cggatacagc
    25081 cgtagtttgt ttgcagccct taatatcaac gatatccgca gcatcacttt attgaccggc
    25141 tccgatgcag ccaaatacgg ctctctgggt tcaaacgggg tgcttctcat cgaaacggaa
    25201 caggccacca gcgataacct gaatacgcgt atttcgcttt gtacacagta cggactgcat
    25261 ttcaaaggcc gtaccgtcga cgtactcgat gccggcggat acaagaatta tcttcgttcg
    25321 gtaggtctgt cccgctacgg gcaaatgggg cctctcacca ccgattatcc ttttctgcaa
    25381 acttcgtccg actaccccag cgcttacatc ttcaacaaca acaccgactg gcagtctgaa
    25441 atttaccgtc cggctctggt caccaacaat gttttccgtg tagaaggcgg cgacgaaatt
    25501 gccaaataca atatgagcgt gggctactct tcggacggag gcatactgta cggcacccgg
    25561 accgatcgtt accatacatt gatcaactcg gacataacgg tcagccgcaa aatcgacatt
    25621 ttcagttcgg tcggactctc ttacttgcgc agccaattgc aggaacaagg catgaacaaa
    25681 gaaaccaatc ccctgctggc agcctggttt aagatgcccg tactcaatcc gtattttgcg
    25741 ggcagcgacg gagaattgac agggatttac gctccgtaca atttttcgaa catcaacgat
    25801 taccctcaat atccctacga aaacatcagc aatccaactg ctattgtaaa tacactggaa
    25861 gccgccgaca aagtttacga tgtcaacatc ctgctcggac tgaattacca actgaccaac
    25921 aagattaaac tcacggcgca atacaaccgg caataccggt atacccaaga agacctgttt
    25981 atacccggca caaacaacca ggccatttat ccccaatact acggaatcgg acgcaacacc
    26041 gtacgcaagg ctgtggccga aaaccggaac agcttctacg gtctgaacgt ctcttaccga
    26101 aataccccga atcggattca ccaatgggag atcgacgccg gaggccgcat tatgagtacc
    26161 tctgccgaat acgatatgtc ttcgggctac aatacggcca acgatttcta caaaaccctc
    26221 gatatgacca cagacgaaga atattccgac ggttatatca gtcagtggct ctgggccaac
    26281 tactacctca ataccggata tacctggaaa gaatggctgg cggccaatct aaacttttcc
    26341 gtggacggat cttcggtaag cggtatcgat gctccgcggt attacgttta cccggcaggc
    26401 gccttaaaat ttttggcagc ccgtctttcg tttattcccg aatggatcga ccggctcgat
    26461 ctgagtgccg agtatgccct gacgggaaac agccgttttt cgagtaacta cgccaaaaac
    26521 tactacaaca gctccaattt cttcgctatg ggatccatta tacgcggtga tattcccaat
    26581 accctgctgg aacccgagaa acacaaccaa ttcaaagccg gaatcgatat gagtttgttg
    26641 aacaaacgcg ttcaactgag cgcagccgct ttcaattcct acgcttacga tttgattatt
    26701 cctcaaaata tttcgagcat ctatgctgcc gataattatt acgagaactc agcagccgtt
    26761 tcgaccgacg gcctcatact gagtgttcgc ttcgtacctg tcgaaaccaa caagttcagc
    26821 tggaccctgg gcgggaacct tacaacgaat aaaagcgtag tcaaagattt gggagaacag
    26881 acttatatgg atatatacta cacccaattc tccgaagggg aagacgccat agtaagactg
    26941 gaagtaggtg aaacgcccta tcagttttac ggatacaaga cgaatggaat atacgaaaca
    27001 agcgcggaag cccgggcaga caactacacc tctgtgtaca acagtaaata caaagccggg
    27061 gatatccgct ttgtcgaccg gcacgcagac ggaatcatca acgaacaaga caaacagttg
    27121 ctgggcagta ccagccccga ttgtttcggt tcgcttttca cccaactgcg cttccggcaa
    27181 atcagtttaa acgctcaatt taattaccgc atcggaggca aaatttacaa tgccgtcagg
    27241 cgccaactgg aatcgatgga taattttcat aaccaatcgg tggctgtgct caaccgctgg
    27301 caggcagaag gacagaaaac caccatgcct cgagctgtct acggtgaccc cagtggaaac
    27361 aacgtctttt ccgatcgctg gatcgaagac ggctcttata ttaaactcgg cagcctcagc
    27421 ctaagctatt ccattgatca acctatcggc aaactgttcc gttccggtac cgtctggata
    27481 agcggagaaa acctgctgac ctttaccaat tacctgggca acgaccccga attggcttac
    27541 aactacgacg aagctcttta cggtatggat ttcgccaaag tggccaatcc tattactctt
    27601 aaaatcggat tcaacttgaa cttctaagcc ctatgaaaaa atacctgacc atcctgagtc
    27661 tgtccgccat tttaggcttg acatcctgcg aagcaatgtt catcaccgac ccagacgata
    27721 tcatcaacgt agacgattat atcagcgaag acgatgaaat gtacaaaggc tttctcggca
    27781 ttatgacgcg catgcagcag tcggccgacc attccatcat cctgaccgat accagagggg
    27841 attatttcga ggtcactccg aacgcgccgc tcgaattgca aaaaatttac agttacgaac
    27901 cgaccgatgg caattcctat gccgacccgg ctccctatta tgcagtggtc atagcctgta
    27961 acgactacat cgacaaaatg caggactacc gcgaaaaagc cggcgaaaac atggacaata
    28021 attcctcaaa gaatttcaat gcccttatca gcagcaccat acgcctaaaa gtctggacct
    28081 atcttaaact cggctccatt tacggcgaag cgatttggtt tgacaacccg ctcgaagaga
    28141 agatagattt atccgaccat accgtattca gccgttgcaa catgaaccaa atcgtcaacc
    28201 gttgtcttca tttgctggac agcggactgg tgctctataa aaacgaagtg atttcttcca
    28261 aaataattat ggattgggct tcgtggataa acgaagaaaa agtgaccgat aattacatac
    28321 actggcaata tatgattccc gaatacttgc ctttacgctg cgaattgctg ctctggcggg
    28381 gacaggaaga ggacttcagg tgggtaagag acaacataat ggactacctg ttcaaagtcc
    28441 acatgggaac catcaacgac ggtgtaaacg tagcctttaa cgatttccgc tatgcctgca
    28501 acatccccat ccggaccggg gcagacaacc tttacgctac cgaatattac cggatgttct
    28561 acaacgagct gtacaatgcc agcaacttta ccaattttta ccaggtaatc accggcatca
    28621 tgtacgatta caaaaaccat cagaccaatc gtatcataga atatttttgc ccgcgcagcc
    28681 ccgggaaata ttatttacgc ccttcctcct acgctgtggc aaagtatccc gaatcggaca
    28741 tcagaggtat cgtacaacgc aacaatatgg acgtaataga cggagatact tgtttctgca
    28801 agtattatta tcaccggggt gaatggctcc gtacccgtat agtggaaata cacccggtca
    28861 ttcccttgtt cagggggcac gactaccatt tctacctggc cgaagccgaa aatcatctgc
    28921 atcagtggca tcaagccgaa gtgataatga atgccggtgt taccaatgaa tttgccgaca
    28981 aaatcctgcc caattactgg aacagcaatt actccacctg gttctgccct gccggcggtt
    29041 acggagatat cggcgtagcg ggctgtgttt acggaaagat gcgtgattac ccggttcgca
    29101 atctcgtgaa gaccgacaaa acttccgacg ggacaggccc tttctacaac agtaaaaacg
    29161 aagtttttga cgaagatgcc cgcatgcgtg aatacgatct gctcattatg gacgaagctc
    29221 tggcagaata cgccggagaa ggcagagcct acgctattgt ttgccgcatg gccgaacgtt
    29281 acggtgctga ggcggtggta gatcgtatcg ccgataaata caagggtggc ccctattacg
    29341 accaggttcg caacgctatt ctaagcggcg actattgggt cagatgggac cttaaagcag
    29401 acgatctgaa ctgattttga gaatccggac attaaatccg tccacaacca tgtaggtatg
    29461 aaaagacttg tcgtttacac aacattcatt ctgctgtttt gcttttgtgt tcgggccgaa
    29521 ctaaccgtta tcagggccat accccccgac ggcagcaccg gagtcgacat caacagccgg
    29581 gtggtgcttg ttttcgacaa gaacatttcc gaaggccgag gcatcgcctg cagcttaaac
    29641 ggcacagttg tcgtacccgc tgtttcctcg aaaatggcca ccttcatcct tccctgtatg
    29701 gattattccg tcagttatca attccacgct cctgccggag cttttatcga aaaagataat
    29761 ccttcgaatc tttctcccga aatcagactg acatttaccg ccaagagcag gcctcaaccc
    29821 gaagccaggc tttttgacgc catcgtctcg gcagacagca ccggtgatta tatcagtgta
    29881 caggcagcca tcaatgcggc tcccgaaaaa cgctccaaac cctggctgat tttcgttcgc
    29941 aacggagttt ataacgaggt gataagggtg cccgaatcca aaccatacat tcatcttata
    30001 ggcgaaaatg ccgacagtac cattatccgg tataaaatct gctgcacttt tttggtggac
    30061 ggaggctaca cagggccttt ggaagaagcg gaagcctttg gggcctacgc tgctcaattc
    30121 agcgccggct atgccagggg ttctctcgga attgacgcca tggcccatat tgatgccaaa
    30181 aatttttatt ccgaaaatat ctcttacatc aatctctggg gctacgaaga acgcaattat
    30241 cctcaggctc tggccgtttg gtgtaccaaa gaccgcaccg ccttttacaa ctgcaaagcc
    30301 ctgagctacc aggatacctg gcgtacaccc tccagcggcg gttaccgcca atacctcaaa
    30361 aattgcctga tcgaaggaaa cgtagatttc atctacgaca acggcgatgt cttttttgat
    30421 tcctgccaga tcaatattat aaaaaacgac aaagccaccg aagaagccgg ctggattgta
    30481 gcgccctcgc acagtgggac caaatggggt tatgtgttca aaaattgcgg cctgagcgcc
    30541 gaaaagaaag gcgattctat ttatctggga cgtgcctgga tagcccaacc caaagccgta
    30601 tttatcaatt tacaactcga cgacaaggtt gtgttgcggc ccaaaggctg ggacagtcat
    30661 ttcggccagg ccatacccat catttttgcc gattggggta cccgcaatgc agacggcagc
    30721 ccggttgatc tgagccaacg caacaactgg tattggtact tgaacgggaa cgacaccgtt
    30781 cagggctata gccggcaata cctgagcgat gagcaagccg ccacctacac cttccataac
    30841 gtcatcccag gctccgacgc ctggcggccc gacctgatgg cagagcgtct gcccgctccc
    30901 catctgaaaa tagagtccgg tctgttgagt tggaacagcg ttccttttgc catctgttac
    30961 gaaattcgtt acaggggtaa atttgtctgt ttcacaacag agaacaacta ccggcctgca
    31021 agcgccgcca cagactatca ggttcgcgca gtaaacgaat ggggaggcag aggcctttta
    31081 tcggactacg ccacaggttt aaaatccaca tccccggcca ctgcgacaat agtccgtact
    31141 gatttttttg atcttatggg gaggatgatc cccgaaaaca acatttccac cgggaaacag
    31201 ctcgttttgc gtaaaataag ctattccgat ggaaatgtag tggtagttaa ggaaataaga
    31261 taatccttta ttcttcggga acgaccagtt tatacccctt tccgtgaata ttcataatct
    31321 gaatatcggg atcgtctttg aggtgtttgc gcaacttggt aatgtaaacg tccatgctgc
    31381 gggcattgaa ataggtattt tcgccccaga tggtcatcag tgcataattg cgctccagaa
    31441 tctggttttg gtgactgcac aaaagggtga gtaactcaca ttccttggtg gttaaacggg
    31501 tttgtttatc accgatggac aaaatttgct tgggcgtgtc aaaagtaaat ttaccaatat
    31561 gataaagggt tacttctttg gcttttttac cttttacgcg cctgataata gcttcgatgc
    31621 ggaacaagag ctcttccata ctgaaaggct tggtcagata atcgtctgca cccagtttaa
    31681 atccggtcag aatgtcctct ttcatgtttt tggcggtcaa aaacacaatg ggggtatcgg
    31741 cattcatcat ccgaatgtcc tgcgccaggg taaatccgtc ttttttaggc atcattacgt
    31801 ccagcacaca aaggtcgtac tttgttttct ggaaagcctt gtaaccttgc tctccgtcca
    31861 acaaaaggtc aacaatatat cccttggcct gcaaatattc gcagagaaga gatcctaaat
    31921 ttccatcgtc ttcgcacagc attatgtgca tcttttcttc tgtcatagct gtttttttat
    31981 taagggtaaa tgaataatga aacttgttcc tacattgagc tcgctttcgg cggtgatgca
    32041 gcctttgtgg tcactgatta tttttttgac gtaggccagg cccaaaccga aacctttaac
    32101 gttatgaatg ttgccgctgg gggcacgata aaagcgttca aatacttttt tgaggta
//
LOCUS       DAOSTD010000011        31579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1575_length_31579_cov_14.542761, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000011 DAOSTD010000000
VERSION     DAOSTD010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31579
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1575_length_31579_cov_14.542761"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLI37_01355"
     CDS             <1..405
                     /locus_tag="PLI37_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ57699.1"
                     /translation="SYIYSNLLLQAINTPPRNVQLEGASPFIVNVDLAYNYSYDYINI
                     TNSLVFNYFSSRVHTIGAQGYKNIMESGVPTLDFVSLSKISRHLTLKLKASNLLNSTF
                     RLTRENSSGSHNEILSQFQKGINVSLGLAYDL"
     gene            456..1625
                     /locus_tag="PLI37_01360"
     CDS             456..1625
                     /locus_tag="PLI37_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57700.1"
                     /translation="MKKYFMNLMVAVAATTLLFSSCEEKEVKEPIDILIEEGVLRGIL
                     THEVTLDAGKTYDLAGAFIVEKGGSITIPAGTKIVAEEGFGNYILVAQGGKIFVEGTA
                     ESPVKMVGKTTEAKAGFWGGLIINGYAPLTSSQQEAKTEINNDYSYGGNKTNDNSGSI
                     TYLILESTGARSSADIEHNGLTLNGVGNGTKIENVFVYECADDGIEFFGGSVNVTNLL
                     VVNTDDDMFDFTQGYNGTLKNAYGIWEQGFTSSESDPRGIEADGNFDGNYKDDTNQSN
                     FRVENMTIDLRLDHNNDKTTQMQDVIKVRRGAKATIVNALVKGTGATGDLIDLTDKKG
                     DGDTSSSISLTNSLTNPNAGKEINAKAETPNVKIEAGNTGCPTNIFSWTGYNFSK"
     gene            complement(1751..2656)
                     /locus_tag="PLI37_01365"
     CDS             complement(1751..2656)
                     /locus_tag="PLI37_01365"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HOQ57701.1"
                     /translation="MSSKLSSKNDLPGKSTAFISVILKGLGQIMLQENSITGLLFLIG
                     IFYGSIAMGFAALLATVCGTAVAYLLKYDHAEINQGLYGFSAALVGVAVMLFLKPVFW
                     SWIIVVIGAVLAAMLQHFFIKRKIPAYTLPFVLVTWLILFVCNLFNSGLFTVSAPAIA
                     EVSDSLTDGFKSFGQVIFQSSLLSGILFFLAVFISSPISALYGLAAAIISAILAFYVS
                     VPINDINLGLYGFNAVLCAIVFAGPKVKDGMWVLISLILALVISLLMLKIGITKLTFP
                     FVLATWVTLFLKNKIFLRKSIAYDK"
     gene            2764..3567
                     /locus_tag="PLI37_01370"
     CDS             2764..3567
                     /locus_tag="PLI37_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HOQ57702.1"
                     /translation="MSHKTEKFHLLIVLLTVLFFACTSKQEQVTRLFLIGDSTVADYA
                     NNYDPGKDYYKTRYPVTGWGQVFQELFVKEELKNFEHLFDSDSVLIDNRARGGRSTRS
                     FFEEGRWRSVYEQLKPGDLVLMQFGHNDAAVDKVERYVSVGGYKEYLRMYVLQTRAKK
                     AIPVILTPVCRNYPWKDGRLENVHGEYPQAAKEVAEEMDVFFIDLNQLSMDAFSEIGR
                     DYVSSRYFMNLPAGVYEAYPDGQSDNTHFQPEGARVVAGLVFEALKALK"
     gene            3674..4663
                     /locus_tag="PLI37_01375"
     CDS             3674..4663
                     /locus_tag="PLI37_01375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ57703.1"
                     /translation="MNKRRLGKTGFDISEISLGTWQVGGRWGSPFSHSNADAVLNTAV
                     DEGINFIDTADVYGDGESEKAVGRLLRSRSETIYVATKCGRKLNPHIDASYQVDVLRK
                     FVEDSLKNMGLDTIDLIQLHCPPTETYDRPEIFALFDTLKKEGKIKHLGVSVERVEEA
                     LKAIEYDNVCTVQIIFNMFRQRPAEDFFARAAQRDVGIIVRVPLASGLLSGKFTINTS
                     FGKDDHRTFNRQGEAFDKGETFSGVDYAQGLAAVEEIKKLFPAGQPLSTFALRWILMF
                     SQVSTVIPGASGPEQVQSNVQATKLPALTEEQMQGIRKIYRDRIWPLIADEVW"
     gene            complement(4785..6473)
                     /locus_tag="PLI37_01380"
     CDS             complement(4785..6473)
                     /locus_tag="PLI37_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOQ57704.1"
                     /translation="MKPAIFPLSLLFSLLMLFSLNPTQLQAKELTKAKNTTVPLYRLF
                     EASISNNKAYQNRFTDVELLVSYQAPSGRRLEFRGFFDGDGKGGGDAQNGKIWKMRFM
                     PDETGIWQYQYRWSDDSPGGKGSFNCTAEGAGKGVLQAYKENPHWFAYNGSDPVYLKS
                     YYETGHGSIGQDFDWIKEHVYGRLIEHGYNHLQVNWLLSLCCFGQYYKDGPEPECSDL
                     TLYEEGKASSTMNLDVWKRMEQHLGFLNNKDIGVHMFLGVDGSRNDGPAWTALSTGEK
                     DFYVRYMVARLAPYANLAGWNYVWEVPGNREEQELGFARLVSQYDIFNHLRTYEDEFP
                     REHYYHLPEYSFAAIENHQIAAPGKNLERHLWRDAWTHHMACLLGYAGKPVFMSEGNA
                     LWRRYWHERTGATQDDLRRSAWACATAGASFTWNGHASEYELYAYGPEGLPFSEDNEF
                     KLSEKYVDILTHVMRNEVDFFRMRPHDELLAHQQALRVYALAEPGQQYLVFAPDGESF
                     SLQLEAGNYNRCCWIDSKSGKKQALPPTQSTGPEQAKAFTPPNKDTDWVLVVRK"
     gene            complement(6506..6727)
                     /locus_tag="PLI37_01385"
     CDS             complement(6506..6727)
                     /locus_tag="PLI37_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57705.1"
                     /translation="MAQKFLKINALSSFFQKKSTYFPAHTKIGAPALAQDVREITIFV
                     AFTASLSQAAQTTQATQTTQTTQTTQAYI"
     gene            6792..8495
                     /locus_tag="PLI37_01390"
     CDS             6792..8495
                     /locus_tag="PLI37_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ57706.1"
                     /translation="MKVFLKSCATLVSRKMVFPLFLFLLPLALNAASGLWNSPLAGEP
                     IPYIVTEPESPQKDYKGKSMTVVYLENLGFGKIGQYSNADNVSWLLAQGYRVIELDYA
                     NHSSMAVSLNINRDIIAINNALESGSFCGLNDCSNYRSYILFEGYRIARDVAYFKDDP
                     TVYNYPDGYTEGDSLYMDIIYPANPREPVPVVLSFSYSNSYATYDKNTGELTDANKHM
                     RLFLPYTLGAFDDSFLEGAPARGIAWAMADHPKYCPWGSGKPVGGANDTYKSYQTNPD
                     AAQKVKSAVRTLRAKSQELGLSGKIGIYGFSRGSDAGAMAVGDRSVPEFENAGLHIGL
                     SDDVQAAALGPGVFDFTQIYNTTDDGDRNLETRCPWAWGPLKDNYELWEKMGAAYLVQ
                     SGLSAPVLFFYNTTDSHYYLDQIQKFKEKLEEWAIPPVSLVDYGSGHSVPKDSASLKK
                     LYDFFDQYLTPPSLDEDTTQSSLSAVESKQLPLMLSYNPSREELSVRFSLTQAAQVEI
                     QLFDMAGMLVFSTEKKAREAGLCTELINLNKLGLQQGIYHVRLRAGGLNTVGKFVFCK
                     S"
     gene            8591..11071
                     /locus_tag="PLI37_01395"
     CDS             8591..11071
                     /locus_tag="PLI37_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOQ57707.1"
                     /translation="MNKNFLLGSIVLLFSACCGQSIPVSSPEKNPMRLFYEQPAAIWE
                     EALPIGNGRLGAMVFGDIETEHLQLNEENVWSGGPGNNIQPELKALLPKIRRLIFEEK
                     YQEAQDLALKVLPKDTGTKGNHGMCYQPLGRLFLHQQGEGQVEDYRRELSIDKALLNI
                     KYRRGGIEFRREIFSSFSDDLIVMEISADRPGALDMEIVLNGEHPGHKIETAQSALYL
                     RATSSSRENKKGAVDFTVIVKPRLEGGTLELKEQSLQVSKADRLVLFVSMASNVKDYN
                     DIGGDPELLAQTLMDSAFAKSYEQLRNDHIAVYRPLFERVDLNLGSTDAVNLSTNQRL
                     EQFKEGNDPQLVALYFQFGRYLLISSSMPGTQPATLQGLWTDKLSPAWDSKYTVNINT
                     EMNYWPAEVCNLPEMHEPLFAMLRDLSITGAEAADQMYGARGWCIHHNTDRWRISGAV
                     DGAFYGLWPMGGAWLSQHIWQHYLYSGDRAFLEEYYPVLKGASLFLKDVLQEEPLHGW
                     MVLSPSMSPENSHRSGVSIAAGTTMDNQIIYDVFSNLIDAAQVLNIDLEYADSLRLLR
                     EELAPMQVGRWGQLQEWMHDWDRPNDRHRHVSHLYGLFPSNQISPYRNPELFVAARTS
                     LQARGDVSTGWSMGWKVNLWARLLDGDRALKLITEQLTPAIRPDGSQTGGTYPNLLDA
                     HPPFQIDGNFGCSSGIAEMLLQSQDGALHLLPALPSAWQEGSVKGLRARGGFQVDLSW
                     KQGLADKILIKSTLGGNCRIRSYVPLKGKGLREAKGPNSNPFYQVPEIKAPLMNNEEA
                     LELPELRSFYEYDVSIPEGESLFLQACR"
     gene            complement(11159..13888)
                     /locus_tag="PLI37_01400"
     CDS             complement(11159..13888)
                     /locus_tag="PLI37_01400"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOQ57708.1"
                     /translation="MKTRILSIAFLVLCCLPSTLSQVNISLTLDQETVPMSQDLIGVF
                     FEDINYAADGGLYAELVQNRSFEYYVVDGYTNLGPLTAWSGVQEGGASVALSVVKNHP
                     LNQHNTNCLKLVIYNQGRAAGVKNTGFDGIALKQNENYFFSAYLKRDTDHDKAVVVQL
                     KSSGGYIIASDTIPAIGSEWQQYHLTLKSKITFNDAALYILPQGSGNVYMDMVSLFPE
                     NTFKNRRNGLRADLAQAIADLQPRFVRFPGGCISHGKGLDNAYRWKHTVGDVAARKPN
                     WNLWGYHQSYGLGFYEYFLFCEDLGAKPLPVLPVGVSCQFRNREVAPLEDMGPWIQDA
                     IDLVEFANGDTSTTWGALRAEMGHPEPFHMEYICLGNEEDDIPEFRVRFMMITDSLRK
                     YCPDIKIIGTSGPGDAGSHYTSLWEFSRENQLDVVDEHYYQSTDWFLNNNHRYDNFDR
                     EGPKVFIGEYASRDDRLYNAIAEAAYLTGVERNADIIELTCYAPLFCNDKHQQWHPDL
                     IRFDNTQVVKTASYYVQQMYGTNPGDEYLASQISYDPSFKPEGRDFKGKFGAGTWNTQ
                     AQFDDLTVSDGDNLLFSEDFSAGSSNWQVLSGSFNAAGGVYSQTANNEPALSIFNSAV
                     DTDHYTFRIKAKKTGGYEGFLIPFAYQDQNNYYWLNIGGWNNTQHAVEKVSNGNKSVL
                     VTSAGRINSNQWYEIRLEARGETMECYLDDNRLFAIPMPPGPVTASVSRDNEREELIF
                     KIVNSGNQALTANINITGGSLIDQQVDCQLLTGDATQRNSLAAPELIKPSTYSLAAGN
                     QFQCQLPAYSMQVFRVKEKGSALNKLHQDKSSLLKIIPNPVHDQKVDIVFEKPDRQSY
                     SLKLYDSNGKLIFQKDKVRDKLTSIDTGRLPASTYLVQVVHNQTKYTGQLILN"
     gene            14185..15378
                     /locus_tag="PLI37_01405"
     CDS             14185..15378
                     /locus_tag="PLI37_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOQ57709.1"
                     /translation="MGILKEKFINYTAPQELMAAGVYPYFREIQSDQDTVVIINGKRV
                     LMFGSNSYLGLTSHPKIKEAAIRAIKKYGTGCAGSRFLNGTLDIHTQLEDRLAKLTGK
                     EAALCYSTGFQVNLGVVSVLGGRNDYLILDELNHASIIEGSRLSFSKTTKYAHNDMEA
                     LEQKLKLCEPGHDIIIVVDGIFSMEGDIVRLPEILALAKKYKASVMVDDAHALGVLGE
                     NGAGTAAHFGLTDEVELIMGTFSKSLASLGGYIAADKEVINFIKHNSRPLIFSASMTP
                     ASAATALAALDIMEQEPERIEHLWKLTHYAREAFKAAGFDTGPSETPIIPLLIRDDFK
                     ALKMTRILLDRGVFVNPVISPAVPKGSSLIRFSLMATHSFEQVDEAVDKVSGVAGELG
                     IFDAV"
     gene            15375..16190
                     /locus_tag="PLI37_01410"
     CDS             15375..16190
                     /locus_tag="PLI37_01410"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ57710.1"
                     /translation="MKKVILITGVSSGFGKEAAVLMAQAGYTVYGTVRKTVKPLQGVN
                     ILYMDLTQPASIDKAVAELLEREKRIDVLINNAGMHLGGPIEEAPSELYRRQLDTNLH
                     GMVHLLQAVLPTMRAQNKGLIINFSSIGGLMGLPFQAFYSASKFAIEGLSEALRMELR
                     AFGIKVVLVNPGDFHTSNTANRINVCDQKGPYARQFEISKARFESDETAGGQPAVMAR
                     KLLKIIAAKHPRHRYVVAAPDQKLAVLLKRILPSDWFMPILASYYGIKKRASN"
     gene            16338..18761
                     /locus_tag="PLI37_01415"
     CDS             16338..18761
                     /locus_tag="PLI37_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ57711.1"
                     /translation="MKKTILCLIALLTISLLGAQEKTDAMLFGDVKTLDGKQHLSFIQ
                     IQVKGTNIGTLTDASGHFKIANLPLGKQIIVARGLGFITQEREVFMERGKAVTLYFEL
                     EEDAMNLEQVVITGTRSRHYIKDVPVRTEVITAKTIENKNAANLYRALEGIPGIRVEN
                     QCQACNFTMIRMQGLGAEHTQVLIDGQPLYSGLAGVYGLQQFSTVDVDKIEVIKGAGS
                     ALYGSGAVAGAINIISREPSYQPQTKIETQIGSHGTQRYDLSSSLRNEKGNVGLTIFA
                     QRYSEQAIDETGEGSTREEVRKPDGVSDHVDSELTNLGFSLFVNDAFGGSDQLVLRGR
                     SVFEQRQGGLLTDDYYTNPFTDGTENIVTKRYEASLSYQKKLGLRSEMNFNTAYVNHQ
                     RNATNDSYLNDYLDTHGDSVPDLRIMRPYLADEQTFTSTLSFSSALNTHRLVFGAQFF
                     YDHIDESGMYVVVDESSSYYGDSYRSMATKSAREVGLFAQDEWAIGRWMLVPGLRLDH
                     HRSGESYKADRQVSDLSLFPETSFEATTLNPRLAIRYKLSDRITLRANVGKGFRAPYG
                     FSEDLHLCSGSPRVWKSSGLKPETSVSYNLSADYYGRKIRLSANLFRTDLKNKIGFSD
                     ADAQVAALGYDYQWKNIDDAFVQGLELTAGYSPVSQLHLSMDFSLNRGEYKEEREDWK
                     GTAYEKESRYISRFPSTAGNFMLEYTPANWALVLNSNYQGPMYIDYYNTDIDPLLGDQ
                     SKIKKTEPFVLLNAKVSRRFGQYKLYAGVDNILDYIQDERHLDDAAYLYAPICGVVFY
                     AGLSIDIRH"
     gene            18849..19523
                     /locus_tag="PLI37_01420"
     CDS             18849..19523
                     /locus_tag="PLI37_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57712.1"
                     /translation="MADLVIENPSLLLLLERFGMETALNDTDVAQLCEQYEVNTPVFL
                     LLCNLYNGFFPQRKDINPEQHRLTDIIKYIKNSHRFYTFEKYPQIRDYIAQLYERQCP
                     EDIQQLEIFFGDYFEEVQEHLRYEEEVVFPYCSYLLEGKAKEEINAFSSAEYRDHHTD
                     IETKLADLKNLMLKHLSLDGDFILKRKILYALMELENDLYIHSQVEELVLLPLLEHLE
                     FKDRNG"
     gene            19516..20121
                     /locus_tag="PLI37_01425"
     CDS             19516..20121
                     /locus_tag="PLI37_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOQ57713.1"
                     /translation="MDKCRVNIAILEPSTVLYEGLSTILLKTHHHYYFFRFTDLEELA
                     VMYKLTPFDIVVANPCMLQNKQKAFIRLKKKMPKVYWIALVYSFYDDELLSLFDNKFS
                     ITEDPKVMLSCFERACDSCKCNEKPDKKKLLSERETEVLIALTKGLANKEIAEMMNIS
                     IHTVMTHRKNIVEKTGLHSLPALTMYAISKKLIAFEPGGEP"
     assembly_gap    20218..20317
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20360..21862
                     /locus_tag="PLI37_01430"
     CDS             20360..21862
                     /locus_tag="PLI37_01430"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOQ57714.1"
                     /translation="MALLKEEISRTFNEYLLIPGLTTKDCVPEKIDLRTPLVRYSKGQ
                     SSEIQLNIPFVSAIMQAVSDSNMAIALAKNGGLSFIFGSQPIEEQAEMVRKVKKHKAG
                     FVVSDANLRPDQRLQDVIDLKAKTGFSTIGITHDGSSNGKLLGIVTSRDYRPGKDDPS
                     KIIRDFMTPFSKLVTGRHGISLDEANDIIWENKINTLPIIDKNQCLRYFVFRKDYDNH
                     KKNPNELLDSHKRLLVGAGVNTRDYMERIPKLVEAGVDVLCIDSSDGFSEWQKETIAF
                     VRSKYGDSVKIGAGNVVDRDGFNFLAQAGADFVKVGIGGGSICITREQKGIGRGQATA
                     LMDVAEARDELYRKEGIYVPICSDGGIVQDYHMVLALAMGADFIMMGRYFARFDESPT
                     KKMLIGGNYVKEYWGEGSNRARNWQRYDMGGNPALKFEEGVDGYVPYAGKLKDNLDIT
                     LGKVKSTMCSCGAFSIPELKKEAKITLVSSTSIIEGGVHDVILKDTKGKH"
     gene            21931..23154
                     /locus_tag="PLI37_01435"
     CDS             21931..23154
                     /locus_tag="PLI37_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HOQ57715.1"
                     /translation="MSYKVPVGHRFEATVKKSLAFLCFFTAISFATEAQELDVAVDLQ
                     KDDTGVYNLTIPLPEGVYRVHLVLGSEESSGHTVVRAESRRLYATEIVTEKGEFKEFS
                     FTVHRRSPLISPQEKVRLKPREIGKLNWDDFLNLEISGRQAQLSQLSIEEANEVPVVY
                     LCGNSTVVDQDNEPWASWGQMITVFFDDRLCFANYAESGESANSFISAGRLKKIMTQI
                     KPGDYVLVEFGHNDQKQKGEGKGAYLSYWKSLTEFIDQAREKKAIPVLITPTQRRSFG
                     DDGRIVDTHEDYPQAMRDLAREKQVPLIDLHQMTRVLYEALGTEGSKKAFVHYPAGSF
                     PGQNQDLADNTHFNPYGAYQIAKCVVMGMKQLGLPLVQFLREEVVYDPAFPDDPGSFN
                     WWPSPYVELQKPEGN"
     gene            23178..26186
                     /locus_tag="PLI37_01440"
     CDS             23178..26186
                     /locus_tag="PLI37_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57716.1"
                     /translation="MSICFCLMVGAYAQFYSGGTDPARLKWQQLETENFKLIYPVGID
                     SLALRYAWLLQTAGIHSIEGLRVDAPKKLPVILHPYTTRSNGFVVWAPKRMELFCLPS
                     ADGEPQNWEISLALHESRHVGQMQWAGKGLFSKLYWLMGEQSQGLAAGLFDMFSVGLL
                     EGDAVMAETALSAAGRGRQAEFLMPYKAYFMDSVHFSLDKWHFGSQKHFIPNEYALGY
                     LKLSAGAFLNSSSFAADTVVTASFSGGTASSEMIFPETASSTTAAVFRDITRNPFHYN
                     KTYKQHFGANFYRLFKPAAAFYRQMWLNEAAEKAPFDSLHLIGPVTNRYVSYSSPCFT
                     PQGLLVLKESLSEPLSLVRIDENNKEQRLRTMGELNSSLEACGNRVYWTEQINSPRWT
                     HESYSILLSYDLEKKKIQRLSKKTRYYFPALCEDGGRVAVASYLIGGGSELHILNASN
                     GEIEQVFSVPFGAQLYEMTWDSSGKRIFAVLIRNNGAGIYALDTQTGNWKELMPPQHR
                     KLSGLKYSKETLSFKSDAGGTDNIFRYRLPTGETYRLTNTRFGAFDYLWNKKEDSLYY
                     ISYDTKGYRLAVLAARELNIEEQDFKAVLPGRRVDAILPDGSNVAGLPENPMDLADRF
                     TAESGLVADTMVFPQDFNYRISSYSKERNLFRIHSWAPFYYDLDELSALSFENLHRSL
                     SPGLMFMSQNDLSTSVLQAGYAYRRGFHSGHLKYVYSGWYPVLSVEAHINDREVIRQG
                     LSYPDAEGRRTPSRDTLSAVALDTKFSAYVPLNFDRYGWHRGVVPSVNWQFLNNRYFI
                     GSRYQSHYFNVVQAGLQLYSVKSMAPRDLFPRLGAGLNLQLAATPINRGYFTDLYYAK
                     CYAYLPGLFKNQGLKLTAAYQLHHTKNISFYLGNAVKLRGVDYINPTPKMMAFSLDYA
                     LPVFPDLSLPGFLYLKRIEFYPFVDLLKTLPETSTGTESESLYSSGLETLLNVHLLRI
                     NVELNLGLRCSYNPAKGMVYEMLFSLPYLN"
     gene            complement(26237..26869)
                     /locus_tag="PLI37_01445"
     CDS             complement(26237..26869)
                     /locus_tag="PLI37_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ57717.1"
                     /translation="MHIQPFCFNPIQVNSYLLWDDDLNALIIDPGCYSNREKNTLQEF
                     IQNNKLILRAILNTHLHFDHILGNAFVEKTYAVGSQAHKDDLFLLNQLPLHAQLFGVT
                     AREICRQPVALLEDGDLVHCGNFTLKAIHSPGHSPGSLVFYCENEKCLFSGDVLFRES
                     IGRSDLQGGNGNILFSSLKKLMELPHDTVVYPGHGPQTTIEYERLHNPFL"
     gene            27196..27570
                     /locus_tag="PLI37_01450"
     CDS             27196..27570
                     /locus_tag="PLI37_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ57718.1"
                     /translation="MSRKITNTELSYAIGQKLRQVRMRSQLTQQNVADDVGISATAYS
                     KMENGKTDFSVTRLTQFAQLFKVYLPDFFDENIQVPRYINDSGTVEDYKNKMLEMRAQ
                     KELLKEILEYRLLKSSDKPLSL"
     gene            complement(27539..28159)
                     /gene="rsmG"
                     /locus_tag="PLI37_01455"
     CDS             complement(27539..28159)
                     /gene="rsmG"
                     /locus_tag="PLI37_01455"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOQ57719.1"
                     /translation="MELIKKYFPELNNVQLSQFENLYKLYSGWNQKINLISRKDLDRL
                     YERHVLHSLGIARFIRFKPSSRILDVGCGGGFPGIPLAILFPEVEFVLLDSIAKKIRA
                     AEDIAQQSGLSNVQFRIERAENEKGLYDFVVSRAAMSMPDLYKICRKNLARTSRNALP
                     NGILALKGGDLSAELSGFGSKVLVTELSQYFEEEFFATKKVVYLSF"
     gene            28295..28948
                     /gene="lipB"
                     /locus_tag="PLI37_01460"
     CDS             28295..28948
                     /gene="lipB"
                     /locus_tag="PLI37_01460"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOQ57720.1"
                     /translation="MIDIVHWGRISYSEAWQKQTALFQARIEAKQQGENLLPDLLISC
                     EHNPVYTLGKNGMINNLLLGNNELEQKGVEFFKTDRGGDITYHGPGQIVIYPILDLEH
                     FGLGLKAYINCLEEAVISFLKNRDIQAGRLDGATGVWLQNPARKICAIGVRCSRYITM
                     HGLALNINTDLNYFRFINPCGFVDKGVTSLQAELGGAVQDLNLCRDELMQELKKQFL"
     gene            complement(28971..30065)
                     /locus_tag="PLI37_01465"
     CDS             complement(28971..30065)
                     /locus_tag="PLI37_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOQ57721.1"
                     /translation="MYEKDKRIVMTLDAGGTNFVFSAVQAYREIVEPVRLPSSADNLE
                     QSLDNIEQGFLQVRLQLKQAPAAISFAFPGPADYSRGIIMDLNNFPAYRGGVALGAFL
                     KNRFNLPVFINNDADLFTYGECIAGALPFVNQRLEAAGSAKRYRNLIGFTFGTGFGCG
                     ITSNNELYLGDNGSAGEVLLLRHKTKRDIIIEDGVSIRAIKRVYRELTGDTRELEPLD
                     VFNIADGKQEGDVQAAQKTFEIFGVMAAEGIAAVSTVLDGLIVLGGGLCGARKFFMPS
                     LMQELNGSINTLDGQSKSRLEMKVYNLDQPEELEEFLHPDIQKIKVPRSEELIDYDPQ
                     KRTGILMSKLGTSKAIALGAYAFALHELDK"
     gene            complement(30144..31502)
                     /locus_tag="PLI37_01470"
     CDS             complement(30144..31502)
                     /locus_tag="PLI37_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HOQ57722.1"
                     /translation="MKFFRTVLLLTTLSLFFCNCRKEQDFRLWAPTPPMGWNSWDCYG
                     PTVEEHEVKANADYMAEKLKEYGWEYVVVDIRWFVENDKAGGYNQTDPRYVMDEYGRY
                     LPALNRFPSAADGKGFKPLADYVHSKGLKFGIHIMRGIPVEAVNRQLPIKGAKGITAD
                     QIFSPDLQCAWLRDNYTIQADKPGAQEYYNSIFNLYASWGVDFIKIDDLSRPYHQAEI
                     ELIRKAIDQCGRKIVLSTSPGATPLSAAEHVSKHANMWRMVDDVWDSWWHIAHLMEVA
                     QGWYPYIKEGTWPDCDMIPLGRISIRGERGGDRMSRLSPDEQYLLMTFFCIFRSPLMF
                     GGDLPSNDDFTLSLLTNQEVLRMHRESSNVCQLYRKDNKIAVSSENTATGEKYLALFN
                     MDDRNTQNLAVNVAELGLQGNCRATELWQNQTLTFSDSIHCDLRPHAAVLYKIHPAEN
                     PN"
BASE COUNT         8300 a         6928 c         7699 g         8552 t
ORIGIN      
        1 tcttatattt attcaaacct cctgctgcaa gccataaaca ccccacctcg taacgtgcaa
       61 ttggaaggag cttccccttt tattgtcaat gttgacctgg cttataacta cagctacgat
      121 tatataaaca taactaattc cctggtgttt aactatttca gcagccgggt gcataccatt
      181 ggcgcacagg gatataaaaa cattatggag agcggtgtac cgaccttaga ctttgtttcg
      241 ctttcaaaaa tcagccggca tctgaccctt aaactaaaag catctaacct gcttaattcc
      301 actttccggc ttacccgtga aaacagcagc ggcagtcaca acgaaatttt aagtcaattt
      361 cagaaaggga tcaatgtgag cctgggactg gcatatgatt tatagataac caatagataa
      421 ccaaaaacaa ttttaacaac ttaaaaatga acaaaatgaa aaagtatttt atgaacttaa
      481 tggtagctgt tgcagcaact accttgttgt ttagctcatg tgaagaaaaa gaagtaaagg
      541 aaccaatcga tatcctgatc gaagaaggag tgcttcgcgg aattttaaca cacgaagtaa
      601 cactcgatgc cggtaaaacc tatgatttag caggtgcttt cattgtagaa aaagggggtt
      661 ccatcactat tcctgccggt acgaagatag ttgccgaaga aggattcggt aattatattt
      721 tggtagcaca aggaggaaaa atctttgtag aaggtacggc tgaaagtcct gtgaaaatgg
      781 ttggaaaaac aacggaagcg aaagcaggtt tctggggcgg actcatcatc aacggatatg
      841 caccgcttac cagctcacag caggaagcaa aaaccgaaat caacaacgat tactcgtacg
      901 ggggcaacaa gacaaacgat aattccggct cgatcacata cttgatttta gaatcgaccg
      961 gagcacgttc cagtgcagac atcgagcaca atggtcttac cctgaacggg gtaggcaatg
     1021 gaaccaaaat cgaaaacgtc tttgtgtatg aatgtgccga cgacgggatt gaattttttg
     1081 gaggctctgt aaatgttacc aacttattgg tagtaaatac cgacgacgat atgttcgact
     1141 ttacccaagg ttataacgga acacttaaga atgcctatgg tatctgggaa caaggattta
     1201 caagcagtga atccgaccca cggggtattg aagccgacgg aaatttcgac ggaaactaca
     1261 aagacgacac caatcagtcg aactttaggg ttgaaaatat gaccatcgat ttgcgtttgg
     1321 accacaacaa cgataaaacc acgcaaatgc aggatgttat caaagtacgc cgcggcgcaa
     1381 aagctactat cgttaatgct cttgtaaaag gcaccggagc aacaggcgac ttgattgact
     1441 taacagacaa aaaaggtgat ggcgacacaa gctcaagcat cagcttaacc aattccttaa
     1501 caaatcccaa cgcaggtaag gagattaatg ctaaagctga aactcccaat gttaagattg
     1561 aagcaggtaa tacaggttgc ccaaccaata ttttcagttg gacaggatac aatttctcaa
     1621 aataaaaagt tgattaattc ttaaagagag gctgtcttca cttttgagac agcctctttt
     1681 tttcttcgtt tggttcaagc aataaatgaa gcagtatgtt aagtcttaaa ttcaataaac
     1741 tgaataacca ttatttgtca tatgctatag actttcttaa aaagatctta ttcttcagaa
     1801 ataaggttac ccaagtcgct aaaacaaatg ggaatgtgag ttttgtaatt cctattttga
     1861 gcattagcag actgataaca agagcaagaa tgagcgatat caacacccac ataccatcct
     1921 tgacttttgg tcctgcaaag acaatagcac acaaaacagc attgaagccg taaagtccga
     1981 ggttgatgtc attgatcggt acagagacgt aaaaggctaa aatagcagag ataattgctg
     2041 ctgcaagtcc atatagagcc gaaatgggtg aactgataaa gactgctaaa aagaataata
     2101 tgcccgataa taagctactt tgaaagatca cttgtccaaa gcttttaaaa ccgtcggtta
     2161 gtgagtcact tacttctgca atagccggtg cggatactgt aaataaaccg ctgttaaaca
     2221 ggttacaaac aaatagtatc aaccaggtaa ccaacacaaa gggtagggta taggcgggga
     2281 tttttcgttt aataaaaaag tgctgcaaca tagctgccaa caccgcaccg ataacgacga
     2341 tgatccaaga ccagaatacc ggttttaaga acaacattac tgctactccg actaatgcag
     2401 cactgaagcc ataaagtcct tgattgattt ctgcatggtc atattttagc aggtaagcaa
     2461 cggcagttcc gcatactgtt gctaaaagcg cggcaaaccc catagcaatt gatccgtaga
     2521 aaataccaat cagaaacagt aagccggtaa tgctgttttc ctgaagcatg atttgtccga
     2581 gtcctttaag aattacggag ataaaggccg tagatttgcc cggcagatcg tttttagagc
     2641 tgagttttga actcattttg ttttattttt cggtctgaaa atttttgaag ctgctaattt
     2701 acaaaatgga aataatacaa aacagaaatt ttgtgtaatt tagggacttt aaatcccaga
     2761 tatatgtcac acaaaacaga aaaattccat ttgcttattg tcctgctgac cgtcttgttt
     2821 tttgcctgta cttcgaagca ggaacaggta acccgccttt ttttaatcgg cgattccact
     2881 gtggccgatt acgccaataa ttacgatccg ggcaaagatt attacaaaac ccgttatccg
     2941 gtcaccggct ggggacaggt gtttcaggag ttatttgtta aagaggagct caaaaatttt
     3001 gagcatttat tcgactcaga tagtgtgctg atcgataatc gtgcccgtgg aggccgcagt
     3061 acgcgttctt ttttcgaaga agggcgctgg cgctctgttt atgagcaatt gaaacccggc
     3121 gatctggtgt tgatgcaatt tggtcacaac gatgccgccg tcgacaaagt agaacgttac
     3181 gtaagtgtgg gtggctacaa agaatatttg cggatgtatg tgctgcaaac cagagccaag
     3241 aaagccattc cggtgatatt gactcctgtt tgtcgcaatt atccctggaa agatggccgt
     3301 ctggaaaatg ttcacggcga atatccccaa gccgccaaag aagtggccga agaaatggat
     3361 gtctttttta tcgatctgaa tcaactgtcg atggacgcct tcagcgaaat aggccgcgac
     3421 tacgtgagca gccgttattt tatgaattta cccgccggcg tttacgaagc ctatcccgac
     3481 ggacagtccg acaataccca tttccagccc gaaggggcca gggtggtggc cggtttggtt
     3541 ttcgaggcgc tgaaagcgct caaatagaac agatatcagg ccaatctttt gaattgagcc
     3601 ggttttgagc cctctttggg aaagtttatt tgaaatttaa aggagtatat ttttttgatt
     3661 taaccaggcc aatatgaaca agcgaagatt aggaaaaacc ggatttgaca tttcagaaat
     3721 cagcttaggt acctggcagg tgggtggcag atggggcagc cctttcagcc acagcaatgc
     3781 cgatgcggtt ttaaatactg cggtcgacga gggaattaat tttatcgata cggccgatgt
     3841 ttatggagac ggagagagcg aaaaggcagt aggcagactg ttacgttccc gcagcgaaac
     3901 catttacgtc gcaacaaaat gcggacgtaa actgaatcct catatagacg cctcttacca
     3961 ggtggatgtc ttgcgtaaat ttgtcgaaga cagcctgaaa aacatgggcc tcgataccat
     4021 cgatttgatt cagttgcatt gccctcccac tgaaacttat gatcgcccgg agatttttgc
     4081 cttgtttgat acgctcaaaa aagaaggcaa gatcaagcat ttgggggtta gtgtcgaaag
     4141 ggtggaggag gctctcaaag ccattgaata cgacaacgtt tgcaccgtac agattatttt
     4201 taatatgttt agacagcggc cggcagagga cttttttgcc cgggcagccc aaagagacgt
     4261 cggcattatt gtgcgggtgc ctctggccag cggcttactg agcggtaaat tcacaataaa
     4321 caccagcttt ggcaaagacg accatcgcac atttaatcgt cagggcgaag ctttcgataa
     4381 aggagaaact ttttccggtg tagattacgc tcaaggcttg gccgctgtag aggaaatcaa
     4441 aaaattgttc cccgccggac agcctttatc gacgtttgcg ctccgatgga ttctgatgtt
     4501 cagccaggta agcacggtaa tccccggggc ttccgggcct gaacaggtgc aatccaacgt
     4561 acaggccacg aaattacctg cgctgacgga ggaacaaatg caggggattc gcaagatcta
     4621 ccgggacaga atctggcctt tgattgccga cgaagtctgg taaaagcagc atctgtccgt
     4681 tttaccggca gtatcctgcc cgtttgatta tcgattcaga cgttcaggtt ttgtttccga
     4741 accgggtttt attttcgaac ttcgagctgt ttcccaattt cgagctattt cctaaccacg
     4801 agcacccagt cggtgtcttt gttgggcggc gtaaaagcct tggcctgttc ggggccggtg
     4861 ctttgcgttg gcggcaatgc ctgctttttg cccgacttgc tgtctatcca gcaacagcgg
     4921 ttgtagttgc cggcttcgag ctgcagcgaa aagctttctc cgtcgggagc aaaaaccagg
     4981 tattgttgtc ccggttcggc cagggcatac actcgtaaag cttgctgatg ggccaggagt
     5041 tcgtcgtggg gtcgcatgcg aaagaaatcc acttcgttgc gcatcacatg ggtcagaatg
     5101 tccacgtatt tttcggagag tttgaattcg ttgtcttcgc tgaaaggcag gccttcgggt
     5161 ccgtaagcat agagttcgta ttcgctggcg tgaccgttcc aggtgaaaga agctcctgcg
     5221 gtggcacaag cccaggctga gcgacgcagg tcgtcttggg tggctccggt gcgttcatgc
     5281 cagtagcgtc tccacagggc gttgccttcg ctcataaata ctggtttccc ggcgtaaccc
     5341 aacaggcagg ccatgtggtg ggtccaggca tcccgccaga gatgacgttc cagatttttt
     5401 ccgggagcgg caatctggtg attctctatg gcagcaaagc tgtattcggg caaatgataa
     5461 tagtgttcgc gcgggaattc gtcttcgtaa gtacgtaagt ggttaaatat atcgtattgg
     5521 ctgaccagac gggcaaagcc caattcctgt tcttcccggt tgccgggtac ttcccagacg
     5581 taattccagc cggccagatt ggcgtaggga gccagacggg ctaccatgta acgaacgtaa
     5641 aaatcttttt ccccggtgct gagagcagtc caggccggac cgtcgttgcg gctgccgtcc
     5701 acacccaaaa acatatgcac accgatgtct ttgttgttga gaaaacccag gtgttgctcc
     5761 atgcgtttcc agacgtcaag gttcatggta gaagaggctt tgccctcttc gtataaagtc
     5821 agatccgaac attcgggttc gggtccgtct ttgtagtatt gaccaaaaca gcagagcgac
     5881 aaaagccagt tcacctgcaa atgattgtaa ccgtgttcta tcaggcggcc gtaaacgtgt
     5941 tcctttatcc agtcgaagtc ctggcctatg gagccgtgac cggtttcgta ataggatttt
     6001 aaatacacgg gatcgctgcc gttgtaggca aaccagtgag gattttcttt gtaggcttga
     6061 agtacacctt tgccggcacc ttcggccgtg cagttgaagc tgcctttacc gcccgggctg
     6121 tcgtcgctcc agcggtattg gtattgccag atgccggttt cgtcgggcat aaaacgcatt
     6181 ttccagatct tgccgttttg tgcatcgcca ccgcccttgc cgtcgccgtc aaaaaaaccg
     6241 cgaaattcga gccggcggcc cgaaggagcc tggtagctga ccaaaagttc aacgtctgta
     6301 aaccgatttt ggtaggcttt attgttgctt atcgaggctt caaaaagcct gtacagaggc
     6361 accgtagtgt ttttggcttt ggtaagttct tttgcttgca gctgagtggg attcaggctg
     6421 aaaagcatca acagggaaaa aaggagactt aaggggaaaa tggctggttt cattctggta
     6481 ataatttttg taagatgtta taattttaga tgtatgcttg cgttgtttgc gtggtttgtg
     6541 ttgtttgtgt tgcttgcgtg gtttgtgcgg cttgtgacaa gcttgccgtg aatgcgacaa
     6601 atatagtgat ttctcttacg tcttgtgcaa gcgccggagc tccgattttt gtatgagcag
     6661 gaaaataggt ggattttttc tgaaagaaac tgctcagagc attgattttt aaaaattttt
     6721 gtgccatatt tacgcttgtt ttaatccact gtgcctattg gcacttaagt tttatcgtat
     6781 aacccttagc tatgaaagtc tttttaaagt cttgtgccac tttggtgagc agaaaaatgg
     6841 tttttccgct ctttttgttt ttgcttcctt tagcgttgaa cgctgcttcc ggcctgtgga
     6901 acagtccgct tgccggagaa ccgattccct atattgttac agaacctgag tcacctcaaa
     6961 aggattataa aggcaagtca atgaccgtgg tgtatctcga aaatctcgga tttggcaaga
     7021 taggccagta cagcaatgcc gataatgttt cctggctgct ggcgcagggt taccgcgtca
     7081 ttgagctgga ttacgccaat cacagtagta tggctgtttc tctcaacata aacagagata
     7141 ttattgctat taataacgca ttggaatcgg gctctttttg cgggctcaac gactgttcta
     7201 actacagaag ctatattttg ttcgagggct atcgcatagc ccgcgacgtg gcttatttca
     7261 aggacgaccc cacggtctat aattatccgg acggatatac cgaaggcgac tctttgtata
     7321 tggacatcat ttatccggcc aatccccggg agcccgtacc ggtggttttg tctttttcct
     7381 acagcaacag ttacgccact tacgacaaaa atactggcga actcacggat gccaacaagc
     7441 atatgcgttt gtttttgcct tatactttgg gagcttttga tgattccttt ttggaaggag
     7501 caccggccag aggcatagca tgggccatgg cagatcatcc caaatattgc ccttggggtt
     7561 ccggtaaacc cgtcggcgga gccaacgaca cctacaaatc ctaccagacc aatccggacg
     7621 ccgcacaaaa agttaaatcg gccgtccgca cgctcagagc caaaagtcag gagctcggat
     7681 tgtcgggtaa aataggtatt tatggttttt cgcgtggttc cgatgcaggt gctatggcgg
     7741 tgggcgatcg ctccgtgcct gaattcgaaa acgccggatt acatatcggt ttatccgacg
     7801 atgtacaggc agctgctctg ggtccgggcg tgtttgattt tactcaaatt tacaatacaa
     7861 cggacgatgg cgacagaaat ttagaaacgc gctgcccctg ggcgtggggg cccttgaagg
     7921 acaactatga actctgggaa aaaatgggag ctgcttattt ggtacaatcg ggactatcgg
     7981 cccctgtgct gtttttctac aataccaccg acagccatta ttacctggat caaattcaaa
     8041 aattcaaaga aaaactcgaa gaatgggcta ttccgcctgt tagtctggtc gattacggta
     8101 gcggccattc cgtacccaaa gactccgctt cgctcaagaa gctctatgac ttctttgatc
     8161 aatacttaac ccctccatcc ttggatgaag ataccacgca aagcagctta tcggcagtcg
     8221 aaagcaagca actgcccttg atgttaagct acaacccttc cagggaggaa ttgtcggtaa
     8281 ggtttagtct gacgcaagcc gcccaagttg aaatacaact ttttgatatg gccggaatgt
     8341 tggttttttc aacagaaaaa aaggcgagag aagcaggttt atgcaccgaa ttaatcaatt
     8401 taaataaact gggtttgcag caaggcattt atcatgtcag actgagagcc ggaggcctga
     8461 atactgttgg aaaatttgtt ttttgcaaaa gctaaaagga caaaaattcg cccgctcctg
     8521 tcgtcaaaca tcagtaatat gccggtcgtt ttatctcttg tatgtttcat tttcttaata
     8581 actacacatc atgaataaaa actttctatt aggcagcatt gttttactat ttagcgcctg
     8641 ctgcgggcaa agtattcccg tatcctctcc ggaaaagaat cctatgcgtt tgttttacga
     8701 acagcccgca gccatctggg aagaagcctt gcccatcggg aacggccgtc tgggtgctat
     8761 ggtctttggc gatattgaaa cagagcattt gcaattgaac gaagaaaatg tatggtcagg
     8821 aggccccggc aataacattc agcctgaact caaggctttg ttgcccaaaa tacgtcgatt
     8881 gatttttgaa gaaaaatatc aggaagcaca agatctcgct ttaaaagtgt tgcctaaaga
     8941 caccgggact aagggcaatc acggcatgtg ttaccagccg ctgggtcgcc tgtttctgca
     9001 tcaacaggga gaaggacagg tggaagatta tcgtcgtgaa ttgagtatag acaaggcatt
     9061 gcttaatata aaataccggc gcggcggcat cgagtttcgt cgtgaaatct ttagttcttt
     9121 cagcgacgat ctcatcgtaa tggaaatatc ggccgatcgt cccggagcct tggatatgga
     9181 aatagtactg aacggcgaac atccgggaca caagatagaa acggcccagt ctgccctcta
     9241 tctgagggct acaagcagca gcagagaaaa caaaaaaggc gcggtggact tcaccgtcat
     9301 cgttaagcca aggcttgagg gaggtaccct ggaactcaaa gaacaatctt tgcaggtgag
     9361 taaagccgac cgcctggtat tgtttgtttc catggccagc aatgtgaaag attacaacga
     9421 tatcggcggc gaccccgagc tgttggcgca aaccctgatg gattcagcct tcgccaaatc
     9481 ctatgaacaa ttgcgtaacg accatatagc cgtttatcgc ccgctgttcg agagggtcga
     9541 tctgaacctg ggcagtaccg atgctgtaaa tttgtctaca aaccaacgtt tggagcagtt
     9601 taaagaaggt aacgatcctc agttggttgc cctgtatttt cagtttggcc gctatctgtt
     9661 aatcagcagc tctatgcccg gtacccagcc tgccacctta cagggtttgt ggaccgataa
     9721 actcagtccg gcctgggata gcaaatacac ggtaaacatc aataccgaaa tgaattattg
     9781 gccggctgag gtttgtaatt tgcccgaaat gcacgagccg ctttttgcta tgctccgcga
     9841 tctttccatc accggagctg aggccgccga tcagatgtac ggagccagag gttggtgcat
     9901 acaccacaat accgaccgct ggcgtataag cggggcggtg gacggagcct tttacggtct
     9961 ctggcctatg ggcggtgcct ggctgagcca gcatatttgg cagcattacc tctattcggg
    10021 tgacagggct tttctcgaag agtattatcc ggtactgaag ggagcttcct tgtttttgaa
    10081 agacgtattg caagaagagc ctctacacgg ctggatggtg ttaagtccct ccatgtcgcc
    10141 cgagaactcg catcgttcgg gtgtaagtat agcggcaggt accaccatgg ataatcagat
    10201 aatctacgat gtgttttcca atctgattga cgctgctcag gttttgaaca tagatcttga
    10261 gtatgccgac agtctgaggt tgctcaggga agaactggct ccgatgcagg tcgggcgttg
    10321 ggggcagttg caggaatgga tgcacgactg ggacaggccc aacgatcgcc atcgtcatgt
    10381 gtcgcatttg tatggtttgt ttccctcgaa tcagatttcg ccttaccgca atcccgaatt
    10441 gtttgtggca gccagaactt cgcttcaggc cagaggcgat gtttccacgg gttggtctat
    10501 gggttggaaa gtgaatcttt gggctcgtct gctcgacggc gaccgggcat tgaagcttat
    10561 caccgaacag ctcactcccg ccatcaggcc cgacggcagc cagacaggag gaacctatcc
    10621 caatttattg gacgctcatc cgcctttcca aattgacggc aatttcggct gcagttcggg
    10681 gatagccgaa atgttgctgc aaagccaaga cggagccttg catctgttgc cggctttgcc
    10741 ttcggcctgg caggaaggca gtgtaaaagg cctcagagcc agaggcggtt ttcaggtgga
    10801 tttatcctgg aaacaaggtt tggccgacaa gattttgatc aagtcaacat taggtggaaa
    10861 ttgccgtatc cgttcctacg tgccgctcaa gggcaaaggc ttgagagagg ccaaaggtcc
    10921 caacagcaat ccgttttatc aggttcctga gatcaaggct cctctgatga acaacgaaga
    10981 ggctcttgag cttccggaac taaggtcgtt ttacgaatac gatgtctcga ttcccgaggg
    11041 cgaaagtctg ttcttacagg cttgcagata agccgctgtg ccctttggta aggcaaacgg
    11101 caacatagcc cttctgtctc gcggatataa taacgggcat gataagaccg atgccgggct
    11161 agttcagaat caattggccg gtgtatttgg tttggttgtg tacaacttga accaaatagg
    11221 tggacgccgg caaacggccg gtgtcgatgc tggttagttt gtctctgact ttatcttttt
    11281 gaaagatcag tttaccgtta ctgtcgtaca atttcaagct gtagctttgt cggtcgggtt
    11341 tctcaaaaac gatgtcgact ttttggtcgt gcaccgggtt gggaattatt ttcagcagcg
    11401 aacttttatc ctgatgtagt ttgttgagag cagatccctt ctcctttact ctgaatacct
    11461 gcatggaata ggccggcagt tggcattgaa attgattgcc ggccgccagg ctgtatgtgc
    11521 ttggtttaat cagttcgggt gctgccaggc tgttgcgttg cgtcgcgtca cctgtaagca
    11581 attgacaatc gacttgttgg tcaatcaacg atcctccggt gatattgata ttagccgtga
    11641 gtgcctgatt tcctgaattt acaatcttga agatcagttc ctcacgctcg ttgtctctgc
    11701 tgacggaagc ggtgaccggg ccggggggca ttggaatagc gaaaagccgg ttgtcgtcca
    11761 gataacattc catggtctcg cccctggctt ccagacgaat ttcataccat tgattggagt
    11821 tgattctgcc ggcagaggtt accaaaacgc ttttgttgcc gttacttacc ttttctacgg
    11881 cgtgttgggt gttgttccat cctccgatat tgagccagta atagttgttt tggtcttgat
    11941 aggcaaaagg aatcagaaag ccttcgtaac ctccggtttt tttcgctttg atgcgaaagg
    12001 tgtagtgatc ggtatcgacg gcagaattga atatactcaa agcgggttca ttattggctg
    12061 tttgactgta tacaccgcct gcagcattga aactgcccga gagtacctgc cagttgctgc
    12121 tgccggcaga gaaatcttcg gaaaagagca gattatctcc atcgctcaca gttaagtcgt
    12181 caaactgagc ctgggtattc caggtacctg caccaaactt gcccttaaaa tcccgcccct
    12241 ccggtttgaa agaggggtca tagcttattt gggaagccag gtattcgtcg ccgggattgg
    12301 tgccgtacat ttgttgcacg taatagctgg ctgtttttac cacttgtgtg ttgtcaaaac
    12361 gaatcaggtc gggatgccat tgctggtgtt tgtcgttgca aaacaaagga gcgtaacaag
    12421 ttagttcaat aatgtcggca ttgcgttcaa cacctgtcaa ataggcggct tcggcaatgg
    12481 cattgtacaa tctgtcgtcg cgtgaggcgt attcgccgat gaaaaccttg gggccttccc
    12541 ggtcgaagtt atcgtagcga tggttgttgt tgagaaacca gtccgtcgat tggtaataat
    12601 gttcgtctac gacgtccagt tggttttcgc gactgaattc ccaaagtgaa gtgtaatggc
    12661 tgccggcgtc accggggcct gaagtaccga taattttaat atcgggacaa tacttgcgca
    12721 gactgtcggt gatcatcata aagcgcaccc tgaattcggg aatatcgtcc tcttcgttac
    12781 ccaggcaaat gtattccata tggaaaggtt cgggatgtcc catttctgcc cgcagcgctc
    12841 cccaggtagt gcttgtatcg ccgttggcaa attcaaccag atcgatggca tcttgtatcc
    12901 aggggcccat atcctccaga ggcgctactt ctctgtttct gaactgacag gaaacaccga
    12961 cgggtaatac gggcaggggt tttgctccaa ggtcttcgca aaaaaggaag tattcataaa
    13021 agcccaatcc gtaagattgg tgatagcccc aaagattcca gttgggcttg cgtgccgcca
    13081 catcgcctac ggtatgtttc catcggtaag cgttgtccag ccccttgccg tgagagatgc
    13141 agcctcccgg aaaacggaca aatctcggtt gaagatcggc gatagcctga gccagatcgg
    13201 cccgaagtcc atttcttcga tttttgaagg tgttctcggg gaaaagcgaa accatatcca
    13261 tatacacatt cccgctgcct tgaggcagaa tatacagtgc agcgtcatta aaagtaattt
    13321 tacttttgag cgttaaatgg tattgctgcc attcactacc gatggcagga atggtatcgg
    13381 aagcaataat ataacctccg ctgcttttca actgtactac caccgctttg tcgtgatcgg
    13441 tatcgcgttt gaggtaagcc gaaaagaaat aattctcgtt ttgtttaagg gcaataccgt
    13501 caaagcccgt gtttttaact cctgcggccc ttccctgatt gtatatgacc aatttcaaac
    13561 aattcgtatt gtgttggttc agaggatgat ttttcaccac actcagggcc accgaggctc
    13621 ctccttcctg cacacccgac caggccgtta aggggcccag attggtgtag ccatccacta
    13681 cataatattc aaaagaacgg ttttgtacca gttcggcgta caagcctccg tcggcggcgt
    13741 agttgatgtc ttcgaaaaag accccgatca aatcctggct catgggaacg gtttcctgat
    13801 caagagtcag ggagatgtta acctgagaca aagttgaagg caagcagcac aggaccaaaa
    13861 aggcaatcga aaggattctt gttttcatac tgatttcttg gaataattgt tgatttttag
    13921 cttcacaaat gcaaatatag tgtatttatt tacaaagccc tagcgctttt tgttgccttt
    13981 ttgttacatt ttatttgtgc cggccttttt agtcattttt tatgaattta taatgaagta
    14041 caattcaatc agatatctta tttggtttat attttatgtt tgtttaccgg ctgtatttgt
    14101 tctggatttg aaaattcttc cctatttttg accggttttt ttattcttct gctcgcgtgc
    14161 agaggacaat gaaaaagtat caatatgggg atattaaaag agaagtttat caattatact
    14221 gcacctcagg aattgatggc ggcaggagtt tatccttatt ttcgcgagat tcagtcggat
    14281 caggatacag tggtaatcat caacggaaag cgagtgttga tgtttggatc aaacagttat
    14341 ctgggtttga ccagccatcc caaaattaaa gaagccgcca tccgggctat caaaaaatac
    14401 ggtaccggct gtgccggttc tcgttttttg aacgggactc tcgatattca tactcaactg
    14461 gaagacagat tggcaaaatt gaccggcaag gaggctgctc tttgctacag taccggtttt
    14521 caggtaaatt tgggagtggt ttccgtgttg ggagggcgca atgattacct gatactcgat
    14581 gaactgaatc atgcttccat catagaaggc tctaggttgt ctttttccaa aaccacgaag
    14641 tatgctcaca acgatatgga agccttggag caaaaattaa aactttgtga accgggacac
    14701 gatattataa ttgtggtaga cggcattttt tcgatggaag gagatattgt ccgtttaccg
    14761 gagattctgg ctttggccaa aaaatacaag gcctctgtta tggtggacga tgctcacgct
    14821 ctgggtgtgt tgggagaaaa cggtgcgggc accgctgcac attttggcct taccgacgag
    14881 gtggaactca ttatgggtac cttttccaaa tcgctggctt cgctcggtgg ttacatcgcg
    14941 gcagacaagg aagtaatcaa ttttatcaag cataattccc gcccgcttat tttcagtgca
    15001 agcatgacgc cggcttcggc agccactgct cttgccgccc tcgatattat ggagcaggaa
    15061 cccgaacgca ttgagcattt gtggaagttg acgcattatg ccagggaagc tttcaaggcc
    15121 gccggtttcg ataccggtcc ttcggaaacg cctatcattc cgttattgat cagggatgat
    15181 tttaaagcct tgaaaatgac cagaatattg ctcgaccggg gtgtgtttgt caatccggtt
    15241 atttcgccgg cagtgcctaa gggttcgagc ttgattcgtt tttcacttat ggcaacccat
    15301 agttttgaac aagtggacga ggccgtagat aaagtgtcgg gtgttgccgg ggaactgggt
    15361 atctttgatg cagtatgaaa aaagtaattc tcattaccgg tgtttcttca ggctttggaa
    15421 aagaggctgc tgttttaatg gcccaggccg ggtatactgt ttacggaacg gttaggaaaa
    15481 ccgttaaacc gctgcaaggc gtaaatatac tctatatgga tttgacgcag ccggcaagca
    15541 ttgataaggc tgttgccgaa ttgttggagc gcgaaaaacg tatcgatgta ttgatcaaca
    15601 atgccggtat gcatttagga ggccctattg aggaagctcc ttcggaattg tatcgcaggc
    15661 aattggacac caatctgcac ggtatggttc atttattgca ggccgtactg ccaacgatga
    15721 gagcacaaaa taaaggctta ataatcaatt tcagctctat tgggggcctg atgggtttgc
    15781 ctttccaggc tttttattct gccagtaaat ttgccatcga aggattgagc gaggccttga
    15841 gaatggagct gcgggctttc ggcattaagg ttgtgcttgt caatccgggc gacttccata
    15901 ccagtaatac cgccaaccgt attaacgttt gtgatcaaaa gggtccttac gccagacagt
    15961 tcgagatttc caaagcccgt tttgaatccg acgaaaccgc cggaggtcag cctgccgtga
    16021 tggcccgtaa acttctaaaa attatagcag ccaaacaccc tcgccatcgc tacgttgtag
    16081 cggccccgga ccaaaaattg gccgtgttgc tcaaacgcat ccttccttcg gattggttta
    16141 tgccgatttt agcctcttat tatggcatta agaagagggc aagcaactga gtaaagctgc
    16201 ccgatacgtc gccgaaaacc ggcattaatc cctaaaatcc ggtatatatt ttgcttaaat
    16261 taccctgttt tagcgattgt gagcccattg tgtgtccgtt aaatttgcgt tcgtttttaa
    16321 taggaaattg aacttaaatg aaaaagacga ttttatgctt aattgcttta ttaacgattt
    16381 ctctgctcgg tgcgcaggag aagacagatg ccatgctttt cggtgatgtg aaaaccctcg
    16441 atgggaaaca acatctctcc tttatacaga ttcaggtcaa aggcactaac attggtaccc
    16501 ttaccgatgc ttccggacat tttaagatcg ccaatttacc tctgggaaaa caaataatag
    16561 tagccagggg tctggggttt attactcagg agcgcgaagt atttatggaa agaggtaagg
    16621 ctgttacttt atattttgaa ctcgaagaag acgccatgaa tctggaacag gtggtaatta
    16681 ccggcaccag gagccggcat tacatcaaag acgttcctgt aagaacagaa gttataacgg
    16741 caaaaaccat agagaataaa aacgcagcca atttatatcg ggccctggaa gggattcccg
    16801 gtatccgggt cgaaaatcag tgccaggcct gtaattttac aatgatacgc atgcagggcc
    16861 tgggggccga gcatacccag gtattgatcg atggccagcc tttgtattcc ggtttggccg
    16921 gagtttacgg tttgcagcaa ttcagtacgg tggatgtgga taagattgaa gtcatcaaag
    16981 gtgccggttc ggccttgtat ggcagcgggg cagtggccgg agccatcaat atcatatcga
    17041 gagagccatc gtatcaacct caaacaaaga tagaaacgca gataggcagc cacggtactc
    17101 aacgttacga cttgtcctcg tctctgcgca atgaaaaagg aaatgtggga ttgaccattt
    17161 ttgctcaacg ttattccgaa caggccatcg atgaaaccgg agagggcagc acccgcgaag
    17221 aagttcgcaa acccgacgga gtttccgacc atgttgattc ggagctgact aatcttggtt
    17281 tcagcttgtt tgtcaacgat gcctttggag gcagtgacca actggtgctc agaggacgat
    17341 cggtttttga acaacgtcag ggcggccttt tgacagacga ttattatacc aatcctttta
    17401 ccgacggtac cgaaaatatc gtcaccaaac gttacgaggc ttctttgagc tatcagaaaa
    17461 agctgggttt gcgttcggag atgaatttca acacggctta cgttaatcat caacgcaatg
    17521 cgaccaacga ttcatatctc aacgattacc tggatactca cggtgattca gtgcccgatt
    17581 tgagaattat gagaccttat ttagccgatg aacaaacttt tacatcgact ctttctttta
    17641 gctcagctct taatacgcat cgtcttgtgt ttggagctca attcttttac gatcatatcg
    17701 acgaatcggg catgtatgtg gtggttgatg aatccagttc ttattacggc gattcttacc
    17761 gttctatggc caccaaatcg gcccgtgaag tcggcctgtt cgctcaagat gaatgggcta
    17821 tcgggcgttg gatgctggtg ccgggcctgc gtctggacca tcatcgttca ggtgagagct
    17881 ataaggccga tcgccaggta tcggatcttt cattatttcc cgaaacctcg ttcgaagcca
    17941 cgactctcaa tccccgcctg gctattcgct ataaattgag cgaccggatt acgctcagag
    18001 ccaatgttgg aaaaggtttt agagcccctt acggtttttc tgaagatttg catctttgca
    18061 gcggttctcc ccgggtttgg aaatcttcgg gtctgaaacc cgagacatcg gtcagttata
    18121 atttatcggc agattattat ggccgaaaaa ttcgtttgag cgccaattta ttcaggactg
    18181 acctgaaaaa caaaataggc tttagcgacg ctgatgcaca agtagctgct ctgggttatg
    18241 attatcagtg gaaaaacata gacgacgctt ttgtacaggg cttggagttg acggcgggct
    18301 acagtccggt gagccaattg catttgagta tggatttcag cctgaatcgt ggagagtaca
    18361 aagaggagcg tgaggattgg aaaggaactg cttatgaaaa ggaaagccgc tatatttcgc
    18421 gttttccatc tactgccggt aactttatgc tcgaatacac tcccgctaac tgggctttag
    18481 tgttgaacag caattatcag ggccccatgt acattgatta ctacaacacc gatattgatc
    18541 ctttactggg tgatcaaagc aagattaaaa aaaccgagcc ttttgttctg ctcaacgcca
    18601 aagtatcacg ccgttttggt cagtacaaac tctatgccgg agttgacaat attcttgatt
    18661 acatacaaga cgaaaggcat ttggacgacg cagcctattt gtatgctcca atttgcggcg
    18721 ttgtattcta tgcaggattg agcatagata tacgtcattg attgtacttt gataaaataa
    18781 gatgtatttt tgcttgagat gtaattatgt atcagactca caggacatat atcaagccgg
    18841 atatgtgtat ggccgacctg gtcatcgaga atccttctct cttgttgctc ctggagcgct
    18901 ttgggatgga aacggcattg aacgataccg atgttgctca gttgtgtgag caatacgagg
    18961 taaatacgcc ggtgtttttg ttgctctgta acttgtacaa tggttttttc cctcaacgta
    19021 aggatataaa tccggagcaa caccgattga ccgacataat taaatacatc aaaaacagtc
    19081 atcgttttta tacattcgaa aaatatccgc aaatccggga ttatatagca cagctttatg
    19141 aacgtcagtg tccggaagat attcagcaat tggaaatttt cttcggggac tatttcgagg
    19201 aagtacaaga gcatttacgt tacgaagaag aggtggtatt tccttattgc agttatttat
    19261 tagagggcaa agcgaaggaa gaaatcaatg ctttctcttc ggcggaatac cgggatcatc
    19321 ataccgatat tgaaacaaaa cttgcggact taaaaaattt aatgctgaaa catttgtcgc
    19381 ttgacggaga ttttattctg aagcgcaaaa tactttatgc attgatggag ctcgaaaacg
    19441 acctatacat acattctcag gtagaggaat tggtgctgct tcctttgttg gagcatctgg
    19501 aattcaaaga caggaatgga taaatgtcgt gtcaatattg ctatcctgga accttccact
    19561 gttttgtatg aaggcttgtc gacgattttg ctaaaaactc accatcatta ttattttttc
    19621 cgttttacgg acctggagga attggcggtc atgtacaaac tgactccgtt tgatatcgtg
    19681 gtggctaatc cttgtatgct gcaaaataaa cagaaagcct ttatccgttt aaagaaaaaa
    19741 atgcccaaag tgtattggat agctttggtg tactcttttt acgacgacga gttgctgagt
    19801 cttttcgata acaaattcag tataacggaa gatcccaaag taatgctgag ttgttttgaa
    19861 agggcttgtg acagttgtaa atgcaatgaa aaaccggaca aaaaaaagct gttgagcgaa
    19921 cgtgaaacag aggtgctgat agcgttgacc aaaggcttgg caaataaaga aatagccgaa
    19981 atgatgaata ttagtataca caccgtaatg acgcatcgca aaaacatcgt cgaaaaaaca
    20041 ggtttacaca gcttgccggc tctgactatg tatgccatat caaaaaaact gattgctttt
    20101 gaacccggag gtgagcctta aatacacaaa actttaattc ttttgaatca tgatggtttt
    20161 tgagcaaaaa gccgtaaatt tgcccaattt taatcttgag tattatggca ttgcttannn
    20221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagc aaaaagccgt aaatttgccc
    20341 aattttaatc ttgagtatta tggcattgct taaagaagaa atctcccgaa cattcaacga
    20401 atatttgctg atacccggac taacgaccaa agactgtgtt cccgaaaaaa ttgatttgag
    20461 aactccgtta gttcgttatt ctaaagggca aagctctgaa atacaattga atataccttt
    20521 tgtgtcggct atcatgcaag ctgtttctga ttccaatatg gcgattgctt tggccaaaaa
    20581 cggaggttta tcctttattt tcggctcgca gcccatcgaa gaacaggccg agatggtgcg
    20641 gaaagtaaaa aaacacaaag ccggttttgt ggtcagcgat gccaacctaa ggcctgatca
    20701 gcgtttgcag gatgtaatcg atttgaaggc caaaactggt ttttcgacga taggcattac
    20761 ccacgatggc agttccaacg gaaaattgct gggcattgtt accagcaggg attaccgtcc
    20821 gggcaaggac gatccgtcaa aaattattcg cgacttcatg actccttttt ccaaactggt
    20881 taccggccgg cacggtatta gtctcgacga agccaacgac atcatctggg aaaataaaat
    20941 caatacgctg cccatcatcg ataagaacca atgcctgagg tattttgtct tccgcaaaga
    21001 ttacgacaat cacaaaaaga atcccaacga acttttggac agtcataaac gtttactggt
    21061 aggggcaggt gttaacaccc gggattacat ggaacgcata cccaaattgg tggaggcagg
    21121 ggtggatgtg ttatgcatag actcgtcgga cggcttttcg gaatggcaaa aagaaaccat
    21181 tgcctttgta cgcagtaaat acggcgacag tgtcaagatc ggagccggta acgtggtaga
    21241 ccgggacgga ttcaattttt tggctcaggc cggagccgat tttgtcaaag taggtatagg
    21301 aggaggctcc atctgtatta cccgtgaaca aaaaggcatc ggccggggac aggctactgc
    21361 tttgatggat gtggctgaag ccagggacga actctaccga aaggaaggta tttatgttcc
    21421 catttgttcc gacggcggta tagttcagga ttaccacatg gttttggctt tggccatggg
    21481 agccgatttc attatgatgg gtcgctattt cgcccgtttc gacgaaagcc ccaccaaaaa
    21541 aatgcttata gggggcaatt acgtaaagga atattggggc gaaggctcca atcgtgcccg
    21601 caactggcag cgttacgata tgggtggcaa tcctgcactc aaatttgaag aaggagtgga
    21661 cggttacgtg ccttatgccg gcaaactcaa ggataatttg gatattaccc tgggcaaagt
    21721 caaatcgacc atgtgcagtt gcggtgcttt ttccatacct gaactaaaaa aggaggccaa
    21781 gattacgctg gtctcatcta ccagtatcat agaagggggt gtacacgatg tgatcctcaa
    21841 agacaccaag ggtaagcatt aacgcttgtt ttagcttaat tccgatatct gctgtctact
    21901 tagctcttat attatttatt tggggaagac gtgagttata aagttcctgt aggtcatagg
    21961 tttgaggcta ccgttaaaaa gagccttgct tttctgtgtt ttttcacggc aattagtttt
    22021 gcaacggagg cccaagagct ggacgtggcc gttgatttgc agaaagacga tacgggtgtg
    22081 tataatctga ctatcccttt gcccgaagga gtgtatcgtg ttcatttggt attgggttca
    22141 gaagaatcgt ccggtcatac ggttgtccgg gcggaatcgc gtcgtttgta tgccacggag
    22201 attgttacgg aaaagggaga gttcaaagag ttctcgttta ccgtacacag gcggagcccg
    22261 cttatttcgc ctcaggaaaa ggtcaggtta aagcccaggg aaatcggcaa actcaattgg
    22321 gacgattttc tcaatctcga aataagcgga cgccaagctc agctcagcca attgtctata
    22381 gaagaggcaa atgaagtgcc ggtggtgtat ctctgcggta attccaccgt ggttgatcag
    22441 gataatgaac cctgggccag ctggggacaa atgatcaccg ttttttttga cgacagactg
    22501 tgttttgcca attacgccga gtcgggtgaa tcggccaata gttttatcag cgccggcaga
    22561 ttgaaaaaga ttatgacgca aatcaagccc ggcgattatg tattggtgga gtttggacac
    22621 aacgaccaaa aacaaaaagg agaaggcaaa ggggcttatc tttcgtattg gaaaagcttg
    22681 actgaattta tcgatcaggc tcgcgaaaaa aaggccatac cggtattgat tacacccacg
    22741 cagcgccgca gttttggaga cgacggccgg atagtcgaca ctcacgagga ttatccccag
    22801 gctatgcgcg atttggcccg cgaaaaacag gtgcccctga ttgatttaca tcaaatgacc
    22861 agggttttgt acgaagcact ggggacggaa ggttccaaaa aggcctttgt ccattatccg
    22921 gcaggcagct ttcccggaca aaatcaggac ttggccgaca atacccattt caatccctac
    22981 ggcgcctacc agattgccaa atgcgtggtg atgggaatga agcaactggg tttgccgctg
    23041 gttcaatttt taagggaaga ggtggtttac gatccggctt ttccggacga ccccggcagt
    23101 tttaactggt ggccgtcacc ctatgtggaa cttcagaaac ctgagggcaa ttgaagatga
    23161 ataaaaagct gagccttttg tcgatatgct tttgcctgat ggtaggggct tatgctcagt
    23221 tttactccgg gggcactgat ccggctcgtc ttaaatggca acagctcgaa actgaaaatt
    23281 tcaagctgat ttatcccgtc ggtattgact cgctggcttt acgatatgcc tggttgttgc
    23341 agacggcggg catacatagc atagagggct tgcgtgtgga tgctcctaaa aaacttcccg
    23401 tcatactgca tccttacacc actcgcagca atggttttgt ggtgtgggcg cccaaaagaa
    23461 tggaattgtt ttgtctgcct tcggccgacg gcgagcctca gaactgggaa atcagcctgg
    23521 ctttgcacga atccaggcat gtgggtcaaa tgcaatgggc gggtaagggc ctgttctcta
    23581 agttgtattg gttaatggga gaacagtcgc aaggattggc tgccggtttg ttcgatatgt
    23641 tttctgtggg tttgctcgag ggcgatgccg ttatggccga aacagcctta tctgctgccg
    23701 gcaggggccg tcaggcagag tttctgatgc cctacaaggc ttattttatg gattcggtgc
    23761 atttttcttt ggacaaatgg catttcggct cgcaaaaaca ttttattccc aacgagtacg
    23821 cgctgggtta cctaaaactt tcggccggcg cttttttgaa ttcttcttct tttgctgccg
    23881 atacagttgt aaccgcttct ttttccggtg gaactgcttc ttctgaaatg attttccctg
    23941 aaacggcctc ttctacgaca gctgcggtat ttagggatat tacccgcaat ccctttcatt
    24001 acaacaaaac ctataaacaa cacttcggtg ctaattttta tcgcttgttt aaaccggctg
    24061 ctgcctttta ccggcagatg tggctgaacg aagcggcaga aaaggctcct ttcgacagct
    24121 tgcatctgat cggtccggtc acaaatcgat acgtctctta cagctctcct tgttttactc
    24181 ctcagggcct gttggttctg aaagagagcc tgtccgaacc tttgagttta gttcggatag
    24241 acgagaacaa caaagagcaa cgtctgcgca ctatgggaga actcaattct tctttggagg
    24301 cctgtgggaa cagggtttat tggacggagc aaatcaacag cccgcgctgg acacacgaaa
    24361 gctattccat tttgttgagt tacgatttgg agaagaagaa aattcagagg ctcagtaaaa
    24421 agactcgcta ttattttcct gcgctgtgtg aagacggagg ccgggtcgct gttgcatctt
    24481 atctgatcgg cggaggctcc gaattgcata tattgaatgc ttcaaacgga gaaatcgagc
    24541 aagttttttc ggttcctttc ggggcccaat tgtacgaaat gacttgggac agctcgggaa
    24601 agcgcatttt tgccgttttg atccgtaaca acggggccgg tatttacgcg ctcgatactc
    24661 agaccggaaa ttggaaagag ttgatgcctc cgcaacaccg taaattgtcc ggtttaaaat
    24721 attccaaaga gacactcagt tttaagagcg atgccggagg caccgacaat atttttcgtt
    24781 atcgtctgcc cacaggggaa acgtatcgtt tgaccaatac ccgtttcgga gcttttgatt
    24841 atctctggaa taagaaggag gacagcctgt attatatttc ttacgacaca aaagggtacc
    24901 ggcttgcggt acttgcggca cgagaactga atatcgagga acaagatttt aaggctgtgt
    24961 tacccgggag gcgtgttgat gcgatcttgc ccgatggctc taacgtagcc ggattaccgg
    25021 agaacccaat ggatctggcc gaccgtttta cggcagagag cggtttggtg gccgatacca
    25081 tggtttttcc ccaagacttt aattatcgga tcagcagcta ttccaaggag cgaaatttgt
    25141 tccgaataca ttcctgggcg cccttttatt acgacttgga cgaactgtcc gcactaagtt
    25201 ttgaaaacct gcaccggagc ctgagtcccg ggttgatgtt tatgtcgcag aatgatctaa
    25261 gcacctcggt gctgcaggcc ggctatgcct atcgccgcgg atttcattca ggccatctca
    25321 aatacgtcta ttccggctgg tatcccgtgt tgagcgtcga agcccatatc aacgatcgcg
    25381 aggttatcag gcaaggattg agttacccgg acgcagaagg aagacgaacc ccgagcagag
    25441 atacactctc tgctgtggct ttagatacca aattttccgc ctatgtgcct ttgaacttcg
    25501 acagatatgg ctggcacagg ggtgttgtgc cttcggtgaa ttggcaattt ttaaataacc
    25561 ggtattttat cggttccagg tatcagagtc attatttcaa tgtcgtacag gcagggctgc
    25621 aactttatag tgtaaagtcg atggctccgc gtgatttgtt tccccgtctg ggagccggtc
    25681 tgaatttgca gttggcagca acccctatta accggggtta ttttaccgat ctgtactatg
    25741 ccaaatgtta tgcttatttg cccggcctat tcaaaaatca gggtctgaaa ttgacggcag
    25801 cttatcaact gcaccatacc aaaaacataa gtttctattt gggtaatgcc gttaaacttc
    25861 gtggtgtcga ctacattaat ccgaccccta agatgatggc ttttagtttg gattatgctt
    25921 tgcctgtttt ccccgatctt tcgctgcccg gttttcttta tttgaagcgt atagagtttt
    25981 atccttttgt ggacctgtta aaaaccctgc ccgaaacttc gaccggtacg gagagcgaaa
    26041 gcctctattc atcaggcctg gaaactttgt tgaatgtaca tctgctgcgc atcaacgtag
    26101 aacttaacct cggcttacgc tgctcttaca atcccgccaa aggtatggtt tacgaaatgc
    26161 tgttctcttt gccttatttg aattaatact tacgattgga gatttttcgg ggtttggttt
    26221 ttcaacccgg cacagatcaa aggaagggat tgtgcagcct ttcgtattct atagtggttt
    26281 ggggtccgtg tcccgggtaa acgaccgtgt cgtgaggcag ctccatcagt tttttaagac
    26341 tcgaaaacag tatgttaccg tttcctccct gcagatcgct gcgtcctata ctttctctga
    26401 ataagacatc gcccgagaag aggcattttt cgttttcaca ataaaaaacc agactgcccg
    26461 gcgaatggcc gggtgagtga atggccttga gagtgaaatt accgcaatgt accaaatcac
    26521 cgtcttcgag caaagcgacc ggttgccggc aaatttcccg ggccgtaacg ccaaagagtt
    26581 gagcatgcag cggcagttga ttcaagagga ataaatcgtc tttgtgtgcc tggctgccca
    26641 cggcataggt cttttcgaca aaggcattgc ccagaatatg gtcgaaatga aggtgagtgt
    26701 tcaatattgc tctcaaaatc aacttgttgt tttggatgaa ttcttgcagg gtattttttt
    26761 ccctgttcga ataacagccg ggatcgatga ttaaggcatt gagatcgtcg tcccaaagca
    26821 aatagctgtt cacctgtatg ggattgaagc aaaacggttg aatatgcata atggccgaaa
    26881 atgaaagggg caaaggtatc aaataattct caaaacttcc tttttggctt gtttatcatt
    26941 catcggttcg aaggtaaaac ctgtgagcag agcgaagaaa acctgagcac ataaaatcaa
    27001 ggtgaagaaa accggaacac acagaaagca gggaaaagaa acagaaataa aaaagggatg
    27061 aaaaaaggaa atacaaaaaa cggaaacgaa gaaaatgaaa acacggagaa aaataaaaaa
    27121 taataatata aaaatgttct tcctatgtaa ataatgtgtt atatttgcat tccgaataca
    27181 ttaaccatta ttgatatgtc cagaaagatt acaaacacag aattaagtta tgccataggg
    27241 caaaagttga ggcaagtacg tatgcgttct caactcacgc aacaaaacgt ggccgatgat
    27301 gtcggtattt cagctaccgc ttattccaaa atggaaaacg ggaagacgga tttttccgtc
    27361 accagattaa cccaatttgc acaattgttc aaagtgtatc tgcccgattt tttcgacgag
    27421 aatattcagg tgccgcgata catcaatgac tccggcactg tggaagatta taagaacaaa
    27481 atgttggaaa tgagggccca aaaagaatta ctcaaggaaa ttctggaata tcgcttactc
    27541 aaaagctcag ataaaccact ttctttgtag caaagaattc ttcttcgaag tattgcgata
    27601 gttccgttac caagactttg gagccaaaac ccgacaattc tgcgctcagg tcgccgcctt
    27661 ttaaagccag tattccgtta ggcaacgcat tacgcgatgt tcgggcaagg tttttacggc
    27721 aaatcttgta caaatcaggc atcgacattg cggcccggct aaccacaaaa tcgtagagtc
    27781 ccttttcgtt ttcggcccgt tctattctga attgcacatt gcttaaaccg ctttgttgtg
    27841 caatgtcttc ggctgcccgt atttttttgg ctatgctgtc gagcaataca aattccactt
    27901 cgggaaagag tatggccagg ggaatacccg gaaaacctcc gccgcaaccc acatcgagta
    27961 tgcggctgct tggtttgaag cgaatgaagc gggcaattcc aagggaatgc aacacatgcc
    28021 tttcgtagag cctgtccagg tctttgcgcg agatcaggtt gattttttga ttccagccgg
    28081 aatagagttt gtataagttc tcgaattggg acagttgaac attgttcagt tcagggaaat
    28141 atttctttat cagctccatt tggcggcctt ttgttcttgg ttttttgtga aaaaacggta
    28201 cttttgactt ttttatggtc cgcaggatac gttcttcaaa atacagccta caaaaatacg
    28261 acaatgcagt caataatcaa gaagattact gtttatgata gatatcgtcc attggggtag
    28321 aatttcctat tcggaagcct ggcaaaagca aaccgcattg tttcaggcaa gaattgaagc
    28381 caagcagcag ggcgaaaatc tgttgcccga tttgcttatc agttgtgaac acaatccggt
    28441 ctatactctg ggtaaaaacg gtatgatcaa caatctgctg ctggggaaca acgagctgga
    28501 acaaaaaggc gtggagtttt ttaagaccga caggggaggc gatatcactt accacggccc
    28561 gggacaaata gtgatttacc ctatcctgga tctggagcat ttcggtttag gactcaaagc
    28621 ctatattaat tgcctggaag aagctgtgat ttctttccta aaaaacagag acatacaggc
    28681 cggtcgcctg gatggtgcaa ccggagtctg gctgcaaaac ccggctcgta aaatttgcgc
    28741 catcggagtc agatgcagtc gttacatcac tatgcacgga ttggccttga acatcaatac
    28801 cgacctgaat tatttccgct ttatcaatcc ctgcggtttt gtagataagg gcgttacctc
    28861 cctacaagcg gaactgggcg gagcagttca ggatttgaat ttgtgccggg acgaattgat
    28921 gcaggagctg aagaaacaat tcctctgaaa cgggccgaaa accacagcaa ttatttatcc
    28981 aattcgtgca gggcaaaagc ataggctccc agcgctatgg ccttgctcgt gcccaatttg
    29041 ctcataagta ttcccgttcg tttttgcgga tcgtaatcga tcagttcctc gcttcgggga
    29101 accttgattt tttggatgtc gggatgtaaa aattcttcta attcctccgg ttgatccaga
    29161 ttatagacct tcatctcgag acggcttttg gattggccgt ccaaggtgtt gatgctgccg
    29221 tttaattcct gcatcagcga gggcataaaa aatttccggg ctccacacag gcctccaccc
    29281 aatacaatca gtccgtcgag cactgtcgaa acggccgcaa tgccttcggc ggccataacg
    29341 ccgaaaatct caaaggtttt ttgggctgct tgaacatctc cttcttgttt tccatcggcg
    29401 atgttgaaaa catccaaagg ttccagttcg cgcgtatcgc ccgttaattc gcggtaaact
    29461 cttttaatgg cccgtatgga cactccgtct tcgatgatga tatcgcgttt ggttttgtgg
    29521 cgcagcagca gcacttcgcc cgccgatccg ttgtctccca ggtacaattc gttgttgctg
    29581 gtgatgccgc aaccaaagcc cgtgccaaag gtgaatccta tcaggttgcg atagcgtttg
    29641 gcgctgccgg ccgcttcgag gcgttgattg acaaaaggca gtgcgccggc tatgcattca
    29701 ccgtaggtaa acaggtcggc gtcgttgttt atgaatacag gcaagttgaa cctgttcttt
    29761 aagaaagcgc ccaaagccac gcctcccctg taggccggaa aattatttaa atccataatg
    29821 atgccccggc tgtagtcggc cggtccggga aaagcaaaac ttatggctgc cggagcttgt
    29881 ttcaattgca accgaacctg caaaaaaccc tgttctatat tatcgagact ctgttccaga
    29941 ttgtctgccg acgagggcaa acgaacaggt tccactattt cgcgataagc ttgtacggcc
    30001 gaaaaaacaa aattcgttcc tccggcgtcg agagtcataa ctatgcgttt atctttttcg
    30061 tacataaggc atatggtagt taatgcaggt tcaagagtgt gcagcaagtg gctgtttaag
    30121 cccgattata atcgagtgga cacttagttg ggattttccg ccggatggat tttgtagagc
    30181 acggcagcat ggggtctcag atcgcagtgg atgctgtcgc tgaaagtcag ggtctgattc
    30241 tgccagagtt cggtagcgcg acaattccct tgcagtccaa gttcggctac attcaccgcc
    30301 aggttttgag tattccggtc gtccatattg aacaaagcca ggtacttttc gcccgtagcc
    30361 gtgttttcgg agcttacggc aattttattg tcttttcgat agagttgaca cacattggag
    30421 ctttcgcggt gcatcctgag cacttcttgg ttggtaagca gtgagagggt aaaatcgtcg
    30481 ttgcttggca gatcgcctcc aaacatcaaa ggagaacgga atatacagaa gaaggtcatc
    30541 agcaggtatt gttcgtccgg actgaggcgg ctcatgcgat ctccgccccg ttcgccccgt
    30601 atggatatgc gtcccagggg aatcatgtcg cagtcgggcc aggtgccttc tttgatgtag
    30661 ggataccagc cttgggccac ttccatcaag tgagcgatat gccaccagga atcccagaca
    30721 tcgtcgacca tacgccacat attggcgtgc ttgctcacgt gctccgcggc cgacagggga
    30781 gtggctccgg gcgaagtgct caacactatt ttccgtccgc attgatctat ggctttgcga
    30841 atcaattcaa tctcggcctg gtgataaggc ctcgagagat cgtctatttt gataaaatcc
    30901 acgccccagg aagcataaag gttaaaaatg gaattataat actcttgtgc tccgggctta
    30961 tcggcctgaa tggtataatt gtctcttagc caggcgcatt gtaagtccgg ggagaaaatt
    31021 tggtcggcag taataccttt ggcgcccttg atgggcagtt gtctgtttac tgcttccacc
    31081 ggaatgcccc tcatgatatg gatgccgaat ttaaggcctt tgctgtgtac gtagtcggcc
    31141 aggggtttga atcctttgcc gtcggctgcc gagggaaaac ggttcagggc cggcaaatac
    31201 cgcccgtatt cgtccataac gtagcgggga tcggtctgat tataaccccc ggctttatcg
    31261 ttttcgacga accagcggat atccaccaca acgtattccc aaccgtattc tttgagtttt
    31321 tcggccatgt aatcggcatt ggcctttact tcatgttctt cgaccgtggg gccgtagcaa
    31381 tcccaactgt tccagcccat aggcggagta ggggcccaga ggcgaaaatc ctgttccttg
    31441 cggcaattac agaaaaaaag acttagtgtt gtcagcaaca aaactgttcg gaagaatttc
    31501 atagtatcta aatgttagtg ttcgtttaca ttttggtgtt tcgtttgaat tttggtgttt
    31561 cggtattccg ggagtccaa
//
LOCUS       DAOSTD010000012        30785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1633_length_30785_cov_14.216564, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000012 DAOSTD010000000
VERSION     DAOSTD010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30785
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1633_length_30785_cov_14.216564"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLI37_01475"
     CDS             <1..319
                     /locus_tag="PLI37_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ57723.1"
                     /translation="ALEMANLFYESGFFTSAWADLILVRWESSLKTPSAEKINIYTSG
                     GELILEPPSGSRLLELYSVQGTKLGSYVLNEGKNFKLELPPGMYLYKIYAGKCYTGKM
                     LLP"
     gene            387..1691
                     /locus_tag="PLI37_01480"
     CDS             387..1691
                     /locus_tag="PLI37_01480"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOQ57724.1"
                     /translation="MKKHLFPLFVLLLLTAGCKNAVKPPQSFDYQPDLEAMGIEAEQI
                     QALDSVLQSFVDAQKLNCLAAFAAKGGKVIYYKAFGWKDLENCVPASVDDYYVLFSQT
                     KAITTVAFMTLVEQGLVAIDDKVADYFPEISDQVVTKVNEDGSYETRPVETPMTFVHL
                     MSHTSGLNAGQVGAIRRAERAQMRRADVASGAEAGQRSFDADPRSGTLEDEMIKLAKY
                     PLGFDPGSEWNYHISTNMLAYMVERISGKPLRQYVKETILEPLGMDHTDWYYPEEALG
                     RFVLPGSIVEGKLVAGTDMFVRGAVSRQQSYCEGAIGLNGPIEDYAKFCQMMLNKGEF
                     NGRRILEPETVELMTTINRLPENNSGGGGFQFGLGFELYNENKKPVPAVSNTAYAWGG
                     MLGTEYIIDPENDLIVLYYINMFRNERLYPVYLAAVYDLLLP"
     gene            1950..2996
                     /locus_tag="PLI37_01485"
     CDS             1950..2996
                     /locus_tag="PLI37_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOQ57725.1"
                     /translation="MNRENVITNSKALFLAVILVSTLLFGSCSKSKKPRYEAYLFAYF
                     EGSAGDRQHQEQLRFALSSDAVHWKALNNNEPILISEEISRSGGIRDPHILRAEDGKT
                     FYLVATDMSTGRNGWRSNPGIVMLKSKDLIHWEHSTVHLSKDYPEHFADAYWVWAPQT
                     IYDPAAGKYLVYFTLKLENDDKLDFYSAYANKDFSGFEAEPSLMFSAKHGAIDADIVF
                     KDGTWHMFYKGNTKDEQGREFENGIKQATAPSLQGPWTEHFEYLDVYHNTRTNVEGSS
                     VFKLNDSDTYILMYDLYSSGRYEFQRSQDLYRFTEKPESFVKDFHPRHGSVISITGKE
                     AERLNRKWGGTALE"
     gene            3061..7545
                     /locus_tag="PLI37_01490"
     CDS             3061..7545
                     /locus_tag="PLI37_01490"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ57726.1"
                     /translation="MSKKINFLVLLAFLTAHVFAQHSLEKLDRGLVAVKISNGVFLSW
                     RIPGEEWHYTTYNVYRDGTKLNNEALQVSNYIDAQGSLSSTYTVASVVDGVEQEPSAP
                     VSVWAQAYKELILDLPPGGVTPDNVSYSYTPNDMSAGHLDDDDRYDLVIKWEPTNAKD
                     NSHSGYTGNVYLDGYTLDGDKLWRIDLGVNIRAGAHYTQFMVYDLDGDGIAEIACKTA
                     PGTKDGLGNYLSKGPAATANHSADYRNSGGYILTGPEYLTIFDGRTGAELTTVSYHPP
                     RGSVDAWGDGYGNRVDRFLAAVAYLDGRRPSLVMARGYYTRTVLAAYDFDGTKLTQRW
                     VFDSNTSGNGGYAGQGNHNLSVADVDGDGKDEIVYGSCTIDDNGKGLYNTYLGHGDAM
                     HVSDFDPYRKGFEVFKCLESGDGGTALYDAANGDILIRHKTYNDCGRCMAGNVTNASP
                     GAEVWGGGKRFSATTRQDISGLSLNVSENFRIYWDGDLLSETLNHTTVTKPTNGSTLL
                     QASGCESNNGTKGTPGLQADLFGDWREELVFRTSNPSKIRIFTTTAPGTYRNYTLMHD
                     RQYRLAIAWQNVAYNQPPHPSYFLGEAEGIVSPPPPVMTNGRLVYKGGGVWDNTSEAW
                     TLDGQNTVYSDGRHVFLTGPQGTNDSIELSGTLQPSVLSMASPGRFYLNGENGTLSGN
                     MKLIKQGSGVFSLDGTHDFSGPTEVWDGKMIFSGSLSASPLWLNINTELAAAGHLNQG
                     LEMRYGSKLFVGGEQAAGELNITGTLRLEEKAELVFDFTGNQPGEADVIKLNGDLYMQ
                     NAAVVRILPAFAGGEQFCEGEYTLLTYTGNAQVDISKIILEGIPEIPAGLALVNDTLK
                     LVVRSSREPATILWKGSYSNNWDLVQTKNFWYNDSSDYFVTGDTVIVNDEAGNQVLNV
                     TETVMPSALIFENTGNFVLTGKGEIAGEGTLTKRGSGKLSILNTNSFTGKVLLEEGIV
                     EVKALPNSIDGNGAIGPVSANAALFEINGGTLGVTAATTSDRALTVGSAGATLQTNAN
                     VAWNARISGGRLIKTGNHTLSLSAANTNTELLLKAGTVQLSTEQAQPGRKVIFEGGTL
                     KAFDSSGSYSSASYPLVVEEGQTGRFYVDSRCTYSNTLTGGGTLYIYVPWVRSDFEGN
                     WSNFTGTIVLMNPNAFRNQSTYGYAKAVIDLGTEGVFDDMKSQTVKIGSLKGKGRLWG
                     AGTWEIGYRNEDFTFQGNIETGVIKKVGAGTMTLSSAMTSTANFLINEGGLTVTGLTS
                     GPGTSNVYIKDGAFMSGNGNIQGSIIVEEGGSLYAGLYQPNNLTAGTSFRTANVQMQT
                     GSHFVVKVDPGMEKSDMMVTSGNFVARGNLIMQNISGQPYREGMSFQIVRPGTITGGF
                     TSVEPESPGEGLEWDLNDFTSTGRINVVAASALDKARAAELLVYPNPTAGKLFVQLPR
                     AAGEWRIKLENLDGQLLMEQGAYDSRLLEIDLTHLKQGLYIITVYSGEQWMKRKVLLK
                     "
     gene            7716..8750
                     /locus_tag="PLI37_01495"
     CDS             7716..8750
                     /locus_tag="PLI37_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOQ57727.1"
                     /translation="MRNFLLIALMLGLASCGLQKNKPLTEKDMSAYLLVYFLDNTHSL
                     HMALSEDGYTFTAVNEGKAVVAGDTIAGQKGIRDPHITRGPDGAFYLAMTDLHIFGRQ
                     RGYRDTQWERPQEEYDWGNNRGFVLMKSFDLIHWTHNKVYIQEAFPHLDVGCAWAPQC
                     IYDPQEEKMMLYFTMRLGHGKTQLYYAYTDDDFTRLLTEPQLLFEYPNPAVQILDADI
                     TPMPDGRYCMMYVAQENPGGIKMAVSDKINSGYVYDERWVDLEPGACEAPNVWKRIGR
                     DKWVLMYDIFSIRPHNFGFVETSDFEHFESLGRFNEADMKTTNFSSPKHGAVIQLTKK
                     EAKTLSDYWK"
     gene            8823..9776
                     /locus_tag="PLI37_01500"
     CDS             8823..9776
                     /locus_tag="PLI37_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOQ57728.1"
                     /translation="MILYLLLVVAGFVCLIYGANWLVDGASALAKKYKMSDLAIGLTI
                     VAFGTSAPELVVNVVASINGHSDLTLGNVIGSNNFNTFIILGLSGLIMPIAVQSSTAY
                     KEIPMSLFITVLLLFMLNDFGFSGEGSLSRFDAIVLFALFLLFMYYVFNQMKSDSAPA
                     TAVVVKSNVTIGSLILLGLVGLAAGGHLVVKNSVAIANALGVSEKIIGLTIVAAGTSL
                     PELATSVVAAIKKNSDIAIGNIIGSNIFNISLILSVSAFIRPIAYNPKFNQDLLVLIG
                     GTVFLLMAVLIGKRKQLSRWEAAVLFVSYASYMLYLVMQEL"
     gene            9831..12530
                     /locus_tag="PLI37_01505"
     CDS             9831..12530
                     /locus_tag="PLI37_01505"
                     /inference="COORDINATES: protein motif:HMM:NF019260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HOQ57729.1"
                     /translation="MKRLFLTGCVLSLGFCCVFAQPLAFPGAEGFGANSRGGRGGKVI
                     YVSNLNDEGPGSLREAVEQEGPRTVVFSVSGIIDLKTRLNIEHPYITIAGQTAPGDGI
                     CLRGETLRIAADHVIVRYLRVRLGDAKHGQGSLQGKDAISISSGRHIILDHCSAGWSL
                     DEVLSSSTRTPDLSNVTVQWCFITEGLNPDGHGFGSLIRGTGGARYSFLHNLYAHNRG
                     RNPRPGNYDSNPHDKDPLGLLLDFRNNVVYNWGGEHAAYNADKVSVTRLNFVNNYFVP
                     GQDSKASGIVYSTGSPYNRAYFAGNYYRDELPDDPWKLVKFNENWSQKDISAYKQNQA
                     FESGLVQTDEAPVAYEKVLETGGAVLPKRDAADQRIVKDVRRRTGRIIQSQEEVGGWP
                     ELKSAEAPLDTDLDGMPDEWEKKHGLNPKDPEDRNKLNAEGYTMLEQYLNSIGSFQPV
                     ASGKHRVIVLTDIENEPDDAMSLVRFLCYCNQWDVEGLVATTSVHQQKETAAWRIKEI
                     LQAYGRVRDNLEKHEPGFPTADYLLSVLREGRPDYGMRAVGKGMDSPGSELIIQAVDR
                     PDSRPVWVLVWGGPNCLAQALWKVKETRSPKELEHFVSKLRVYTISDQDDSGPWIRKT
                     FPELFYIASPGFHRYGGYHYATWSGISGDYFHARCAGGDFSLVDNDWVDKNVQNKGPL
                     GEQYPDIKFLMEGDSPSFMFLIDNGLGDPEHPDWGSWGGRYEYYLPKFEKWFLEPETR
                     PLWTNAMDEVLGSDGRWHTGNHATIWRWREHFQNDFSARMDWTIKTYEQANHPPVPKL
                     GHEAYLRAKAGERVNLSAAGSSDPDGDSLRYNWFMYGEPGTFAMGTARTGNPLKINDS
                     DKQEAWFIAPEKARMGTMHIILAVSDDGHPSLTRYKRVIVEIQ"
     gene            12517..13698
                     /locus_tag="PLI37_01510"
     CDS             12517..13698
                     /locus_tag="PLI37_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ57730.1"
                     /translation="MRYNSLNTLFAGSYRKMAAVMKMVGKTLWNIIGLTVALLLLFLI
                     FMIIPQFGKAPSGRRQAGIEASVHYKNGRFHNLEHTPMFVEQSGNKRMFPKFLFRNKK
                     DLRPTFRIPSVATRLKDLPSGQNLLVWFGHSSYYFQLEGKRFLVDPVLGRRASPVPGM
                     LRAFRGTVVFRVEELPPIDYLLITHDHYDHLDYRTIKKLRRKVGVVVCGLGVGSHLSY
                     WGYPASKIIEKDWNESLMLDDKIRLYTETSRHFSGRFLSRNNTLWLSYVIKTPGYSLF
                     IGGDGGYGSHVAEIGKKHGPFDLAVLENGQYNALWRNIHHFPEDVLRSARDLSARRVL
                     PVHSGKFSLAFHPWDEPLTRLSELNRQSPEPVPLVTPLIGQVVELDNENQVFSEWWLK
                     P"
     gene            13772..14371
                     /locus_tag="PLI37_01515"
     CDS             13772..14371
                     /locus_tag="PLI37_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57731.1"
                     /translation="MKTRISFLGKYASLLLLLSLILSTGCSLGSRIYKKYDQVQKVDR
                     YKLDLNYPDFNLRSPLLYMEQSIVKEVSAKESKYSVYDVLVLNSASYRVKEELYIIVE
                     DEIFKPAVTGLEYDNAREIREKSEEVVRADSSTVKVVTGYTENNKKITRFTYTLDNDV
                     IERIRKASIVLFRYYAGPDMVTIKLKGLKLKRVKRLIEK"
     gene            14780..15550
                     /locus_tag="PLI37_01520"
     CDS             14780..15550
                     /locus_tag="PLI37_01520"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HOQ57732.1"
                     /translation="MNENIFNKKGNNTRSLVVVLGAALVLTGLTLLAFNFGWLNPAFK
                     SVIFSWPMLFILFAVVGFSKRQILLPVLFLLIGVFFLLPRLEIVYPGILGEAGRGGFA
                     KNFWPFLLIIIGLMFIVGVATKRKKKVSFKQDVVDTQAVTSGESGWIISDVVFGGLES
                     VFLEPILKGGDIDVVFGGMVIDLRKTTLPDTTVYLNVDAIFGGITLYIPEDWCVKSKF
                     NPVFGGYSDKRASIIVDDKSDSRLVLQGSLIFSGCTVQ"
     gene            15560..17983
                     /locus_tag="PLI37_01525"
     CDS             15560..17983
                     /locus_tag="PLI37_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007366105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ57733.1"
                     /translation="MKKMMLGIKKIWMLSLLMFLSVSVSVAQNDLAETVRGVVVSGDH
                     THELIQASIYVEGSEPGIGTVTDEQGNFTFRVPVGRQKLRFSSVGFLSQEVDILVNTG
                     KEVVLNVVLEPSFIELQGAEIVARHDKSRPMNKLSVTGARTFSTEETFRFAGSLGDPA
                     RMVRSFAGVIPANDSRNDIIIRGNSPVGVQWSLDGVEIANLNHFNTGIGMTGGQVTLL
                     NTNLLSNSDFHMSAWPASYGNALAGIFDLKMRKGNNKKHEFWAQMGWNGFELGSEGYF
                     SKKSTSSYLVSYRYSIPDLMDKMGFYKGITPKYQDLTMKLDFDLNEKNHLSIIGLWGT
                     SGIGFVMSEVTSVDINEDFLQTTDQRIAIDAKTLIAGATHSVEFTPKTKLTTLFSFVR
                     SDTHMPVDTISLIQPNSEWQTLWDENALEDKYSLHSKLEHRFSYNSLIEAGAKYDLYN
                     VKYLEKESFSDERGFFTNVDEKGYFSLARAYAQYRHNITSKLLLTGGAHGMYLQLNKT
                     YAVEPRFGLRYTPASKHTLALAGGLYSQMIPRSFYFIRTLNPEGDIEYSNKQLGFMKS
                     AHGDLSYDWAFAPDWHFKVETYYQWLYDIPVKNNPDATYTMLQIGGAGDNVIMREGNL
                     VNKGTGKNYGAEFTIEKFMSNNYYLLFNSTIYRSTYTNGFNKKEWSTIFDGRYLFNLA
                     SGYELPLKKGWTLFADIKGSLAGGTRYTPVLEDRSRLEHRVVYDNTRINELQVRNYFR
                     IDLRMGYRKNWKRLTDELFVDFQNLTNRKNLYGLFYDINTGTYEEMLLQGFFPMVTYR
                     INFSIGRKK"
     gene            18052..18774
                     /locus_tag="PLI37_01530"
     CDS             18052..18774
                     /locus_tag="PLI37_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57734.1"
                     /translation="MKTKSILSGLFWIAVFYICWGVSQLISIKTQHSLLSSLLFSIVF
                     TGLIGCFIPIYFKNKFHWRYNKSGPDRVVGYLFLILAILFSTILSGAFLKVIELKYSW
                     TLILKYVLLFFPMSLGIGLFAFLLIPNTIQGWEENKTKSVLLIIAIVAFFFLSFYVDS
                     LFQDMELAVTMGFIGLLLGLSYLYIRNFWIVYPALFIIMLVNTLADNKYDEYNYWVVI
                     VSTLLSLTILTFDFIKNRKINN"
     gene            19180..20019
                     /locus_tag="PLI37_01535"
     CDS             19180..20019
                     /locus_tag="PLI37_01535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOQ57735.1"
                     /translation="MENKIIAKYNRPVFFYGLSLLIPWILWFTVAYISHLPGQSSFLI
                     FIQALLAILGLLAPTFVAAYLFLSDKELFNDLKKRFIRQKGFSPIYTILAFSLIFVSI
                     VIAQLISLIFGHSIDQFYISGSPSFTSALLSPWFILLFAPVVEELAWHSYGTDALRQR
                     FNLFTTSIIFALYWAFWHLPLSFIDGYYHSNVVEEGLLYSLNYVFSLFVLIFLMNWLY
                     YKTNRNIFITIVFHCSANVSNEIFATHPDSKVIQTVLLLIVAIVVLFKEKGMFFRKEY
                     QQL"
     gene            20276..22615
                     /locus_tag="PLI37_01540"
     CDS             20276..22615
                     /locus_tag="PLI37_01540"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOQ57736.1"
                     /translation="MRKYFTAIIFVLFCVNSYSQNFTNNVKGVIKDAFTDEPLISATI
                     TLTVDTLKFHTISDNEGKFHFEKVNAGRISLKINYLGYEPYFANNLILASGKELEVEV
                     QMVQSTQSLAEVTIFARERGELNNELAYVSSRSFDVKETERYAGTSGDPARMATYFAG
                     VQAAGDSRNDIIIRGNSPLGLLYMLEGIPIPNPNHFATMGTNGGAISMLNNNQLSNSD
                     FLTGAFPAQYGNATAGAFDLAMRKGNSEKFEYLLQMGTGGLEAGVEGPFSKNRTSSFM
                     VNYRYAFLELFDKIGVNFGTPSIPKYQDLTFKSDFDFGKTSLSLWGFGGFCSMSMDPG
                     KNVLNFTKNLRTEMGGQTNVAAITMKNIINSKSNLRTSLAFTNSRSSMSVDSVFKNSR
                     EFHFFGGVYTENKLIATTQYKNRFNPKNRIIVGASYTHSFVNFADSAKIQNQYVYGTK
                     NRGNYGLAQFNSQLLHSINDRVELTMGVFNQYSLLNNSLSVEPRAAIRYSSGQNSDIS
                     FAYGLHSQTQPGNVYFNQTLTDTINQTYKLTNANLDFSKSHHFVLGYNVNFRRNFNLK
                     TEVYYQNLFNIPVEIKSSHYSVINYGADFYEVVNDSLVNKGKGRNYGIELTLEKYFSD
                     NYYFLITTSLFNSNYLASDNIWRNTVYNSNFVFNALGGYEIKLPKQQTLTINSNVVYA
                     GGLRNIPIDLEKSKMDGYTVYDYKNAYKHKNPDFFKANIKLLYRVNLKRWSYECGFEL
                     TNITNRKNIWRQIYDAETQTIKTDYQMGIMPGGLFRIYL"
     gene            complement(22651..23751)
                     /gene="dinB"
                     /locus_tag="PLI37_01545"
     CDS             complement(22651..23751)
                     /gene="dinB"
                     /locus_tag="PLI37_01545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOQ57737.1"
                     /translation="MQSTPKYRKIIHVDMDAFYASVEQRDHPEWRGKPLVVGSSHPRG
                     VVAAASYEARKFGIHSAMPSKKALQLCPHLIFVPPRFEVYRSVSREIHDIFSEYTDLI
                     EPLSLDEAFLDVTDNKPGIPLAQDIAKEIKIKIKSQLGLTASAGVSYNKFLAKIASDM
                     QKPDGLTVIHPKKALRVIENLPIEAFWGVGKVTAGRMHELGIHTGKELREQPEDYLVR
                     NFGKIGKLFAEFAQGNDHREVEPDRKRLSVGCEHTFDEDIMGKEQIIARFDDLAYELE
                     RRIEKAHFQGYTFTLKIKRGDFKQVTRSQTAEACFTLAREFIELATELLETVNLDNHG
                     VRLIGFSVSNPPADILSETTLNPQLLIPFEEY"
     gene            complement(23766..24581)
                     /locus_tag="PLI37_01550"
     CDS             complement(23766..24581)
                     /locus_tag="PLI37_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ57738.1"
                     /translation="MTEKTYETASGIIHYWTNKINVDAVTLVFLPGLTADHRLFEKQI
                     EYFKDKYNVFVWDPPAHASSWPFKFDFNLMDKAKWLNEILEHEKITCPVIVGQSMGGY
                     VGQTFAELYPEKLKGFISIDSAPLQRKYLTGIELWLLKRMEPVYLLYPWRSLLKAGSK
                     GTAESEYGRKLIREIMMVYDGDKKRYAKLAGHGFKILAEAYEKDLPYKMNCPTLLICG
                     EKDQAGSTIRFNKAWHKDTGIPIEWIKDAGHNANTDKPEVVNRLIEDFVGKLV"
     gene            complement(24666..27074)
                     /gene="ccsA"
                     /locus_tag="PLI37_01555"
     CDS             complement(24666..27074)
                     /gene="ccsA"
                     /locus_tag="PLI37_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOQ57739.1"
                     /translation="MEKAKRYLSSYKTTITLLLIYAAVMAVATFIEKDHGTDVAKKWV
                     YYSPAFFLLQFLLVLNFIAAWSRQGYVKQKRWALLTVHLAFVVILGGALTTFLFGKEG
                     TVHIREGSKTDEMIIRTAEGISVEKLPFMLELTDFRLNRYPGSASPSSYESDLLVHVD
                     GRVQEAKVFMNNVLDVKGYRFFQASYDRDELGTILSVNRDVAGRTITYTGYFLLFCGF
                     LLTFFTKNSRLRRLGRQLKSLREESKHLTLALILLIIATVSRAQAAENTPNLLIPPEH
                     AAQFAALPVQSDGRIIPMHTLSSEIVRKVYKELKMGQFNPEQFLLSFLAEPQLWIHEP
                     IIAIDNREISSLYGLPRDWASYAQFFDGNGNYKLLRQMQLIYGKLPAERSATDKDMMK
                     LDERVNIVFQLLDYSLLRIYPDPGDETHTWYPPNVSPSIFPKEDSLFVADCFRNYLAE
                     VSRSLQSGEWSKPEQLVAEIREFQLKNDIGAHIDVGKIQAEIRYNRMRIFDRCKLSYF
                     ILGGLMLVLAFMQLLKKQKWADTTIKVLSVAVLCAFLIHAFGMGMRWYIGGYAPWSNS
                     YETMVYVAWATVLAGVVFGRKSTITMALATLFGGIILFVSGLNWMDPQIGTLVPVLKS
                     PWLMFHVAVIVAAYGFFGIGFLLGIVNLSLTAFAKKSELIRFRIKELSITNNMALLVG
                     LTLMTIGTFLGAIWANESWGRYWGWDPKETWALITMIVYSIVTHIHLVKKRDNLWLFN
                     LLSVLAFASVLMTFFGVNYLLSGMHSYGVTDGVSTVFIYIVLAFVTVGILGVLSYRKR
                     RHES"
     gene            27365..28201
                     /locus_tag="PLI37_01560"
     CDS             27365..28201
                     /locus_tag="PLI37_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ57740.1"
                     /translation="MIKIRDLRFSYGNNVVLKDISMELQEGRIYGLLGENGVGKTTLL
                     TLLAGLKEPGAGSIEIDGQKPFDRTPSFLSQIYYLPDEVPAIRMKAIDFAMDRGQFWV
                     NFDPDKFKEIAGVLETDTSMRMDQMSYGQLRKTYISFALACNTRYLFMDEPTNGLDIP
                     SKSQFRKAMSKYTSDESTLLISTHQARELEAIIDPIIILDRKDVLLNATLSEITKKIY
                     FDYSTATSPEALYSELIPGGCIQVLVNKSGEESKVNLEALFNAVHRHKELIKKMFNDE
                     IA"
     gene            28204..29073
                     /locus_tag="PLI37_01565"
     CDS             28204..29073
                     /locus_tag="PLI37_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57741.1"
                     /translation="MGSNVLNMNRSRRYFRSDLRAAVSNYGITFLVFSTLAVTTYLLH
                     GLFFLTTSGEWYAMSSSLRIALFVISGLTVLISSPAKLYGHLTDRREGSAFLMVPVSR
                     LEKFVSMVLISCIIIPFSFGLIYLTLDSLVCLIGPACGDSLFQIIFKSSPSFINTIPD
                     LHIYKESFRSILTPWLYFDDIIQIALLFLLGALIFKKSKTGKTLGSLIIISISLNLLF
                     TPIMSLAMFDKFRMMAEMMSDANITLEAIEQMFPFFSWILRNLAIVDTLSDTLVNGLL
                     LFFVWLRLKKLKH"
     gene            29092..29430
                     /locus_tag="PLI37_01570"
     CDS             29092..29430
                     /locus_tag="PLI37_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOQ57742.1"
                     /translation="MEFNQNKPIYLQIADGILEKILSSELKEGDRILSVRELGAELGV
                     NPNTVMRSYEKLTADGIIYNQRGIGYFVADGARNTSLEKMRADFLENELPQILRRLEL
                     LKLDPKDYLK"
     gene            complement(29476..30657)
                     /locus_tag="PLI37_01575"
     CDS             complement(29476..30657)
                     /locus_tag="PLI37_01575"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOQ57743.1"
                     /translation="MKKNDLLTLVLILTVFWACLSSSSLFAQSSDENTSKSVDLSLHL
                     RPAYHTQFGGTPMEFESGGFKFDHIVIDLGGQLTPKLSYRYLQRLNKVSPIFTKENLP
                     STIDYAYLIYAFNERFSMTAGKQALSIGGFEYNKYPVDVYDYSGISNLISCYLTGVQV
                     AFTPTQNQEFSFQIVNNRMGTWREAIGIPQIGLQLEAPTLPLYYSIGWNSNYLDNALQ
                     LRYAANITSPAKGKTLIMVSGGQKWESGPFSIYIDAFYQHSEIDYLGGIRNLTISPQN
                     VVENVNYFSLLGEANYRFQPQWNILLKCFYDDFSTYKSSIFLDKGNCLTSWNYQAGIE
                     FYPNKDDNLHLFLIATSANYLKPTVAFIATPDNSFRISAGFIYRVPVLSLHRRTSPDF
                     R"
BASE COUNT         8495 a         6638 c         7229 g         8423 t
ORIGIN      
        1 tgcgcttgaa atggccaatt tattttatga gagcggtttt ttcacaagcg cttgggcaga
       61 tttaatactt gtacggtggg aatcgtcatt gaaaactccg tctgcagaaa aaatcaacat
      121 ctacacatca ggcggggagc ttatcttaga gcctccctca ggatctcgtc tgcttgaatt
      181 gtactccgtt cagggaacaa agcttggctc gtacgtgttg aacgagggaa aaaacttcaa
      241 actggaactg cctccgggta tgtacttgta taaaatttat gccggtaaat gctataccgg
      301 taaaatgttg ttgccctgag aaatagtatg atacaatttc aatagataaa tggataatcg
      361 agcgaaaatt caaatacttt ttaactatga aaaaacatct tttccccctt ttcgttttac
      421 tgttgctgac tgccgggtgt aagaatgcag tcaagccgcc tcaatctttt gattaccagc
      481 ccgatttgga ggctatgggt atagaggccg agcaaattca ggccttggat tccgtgttgc
      541 aatcttttgt ggatgctcaa aaactgaatt gcctcgctgc ttttgcggca aaaggaggca
      601 aggttattta ttacaaggct tttggatgga aagacctcga aaactgtgtt cccgcttctg
      661 tcgatgatta ttacgtgttg ttttcccaaa ccaaggccat taccaccgtg gcttttatga
      721 ccttggtgga acaaggactg gtggccattg acgataaggt ggcagattat tttccggaga
      781 tatccgacca ggtggtaacc aaggtaaacg aagatggaag ctacgaaaca aggccggtag
      841 aaacacctat gacctttgtg catctgatgt cgcatacttc gggcctgaac gccggacagg
      901 tgggagccat cagacgggca gagagggcgc aaatgcgaag agccgatgtt gcttcggggg
      961 ctgaagccgg acagcgcagt ttcgatgccg atccccgctc gggaacgctc gaagacgaaa
     1021 tgataaaatt agccaagtat cccctgggat tcgatccggg atcggagtgg aactaccaca
     1081 tcagcaccaa catgctggcc tatatggtgg aacgtatttc gggcaagcct ttgcgtcaat
     1141 atgtaaagga aaccattttg gagcccttag gcatggatca taccgactgg tattatcccg
     1201 aggaggctct gggacggttc gtgctccccg gcagtatcgt cgaaggtaaa ttggttgcgg
     1261 gtacggatat gtttgtgcgt ggtgcagtga gccggcaaca aagctattgc gaaggcgcca
     1321 tcggtttgaa cggacccatc gaggactacg ccaaattctg ccagatgatg ctaaacaagg
     1381 gcgaattcaa tggtcggcga attcttgagc cggaaaccgt ggagctgatg accaccatca
     1441 accggcttcc cgaaaacaat tccggaggcg gagggtttca attcggactc ggatttgaat
     1501 tgtacaatga aaacaaaaaa cccgttccgg ccgtatccaa cacagcctac gcctggggcg
     1561 gcatgctggg aacggaatac atcatcgatc ccgaaaacga cctgatcgtg ctttattata
     1621 tcaatatgtt ccgtaacgag cgcctgtacc cggtttacct ggccgctgtt tatgatttgc
     1681 tgttgccgta acaaagctgt ctgatttatt ctttgcattc atactcgtcc ggaaccattc
     1741 ggcaagggat ggtgacggac gttgtatttt ttccctttta gcccgataag ctgaatccgg
     1801 tcgttggaaa tcttcacaag gtgattttaa aagtttattt ctttctgtta taaatgtttt
     1861 aaatgtaata atactaagcc cttgagtaaa taaaaaaatt aaatttgcct tggtttctgt
     1921 tttcatttgg tattctgaat atttacgaca tgaataggga aaatgtaatt acaaacagca
     1981 aggctctgtt tcttgcggtg atcttggtgt caacactgct ttttgggagt tgttcaaaat
     2041 cgaagaagcc ccgttacgag gcttatttat ttgcctattt cgaaggcagt gccggcgacc
     2101 gccaacacca ggaacaattg cgctttgcgc ttagtagcga tgccgttcac tggaaggctc
     2161 tcaataacaa cgaacccata ctgatttccg aagagatttc ccgatccgga ggcatcaggg
     2221 acccccatat tcttcgtgcc gaagacggca agacttttta tctggtagct accgatatgt
     2281 caacaggcag gaatggctgg aggtccaacc cgggcatagt gatgctcaaa tccaaggatt
     2341 tgattcattg ggagcacagc actgttcatt tgtctaagga ttatccggag cattttgccg
     2401 atgcgtactg ggtgtgggcc cctcagacca tctacgatcc ggctgccggc aaatacctgg
     2461 tttattttac gttgaagctc gaaaacgacg ataaactgga tttttattct gcttacgcca
     2521 acaaggattt cagcggtttc gaagccgaac cttccttgat gttcagcgcc aaacacgggg
     2581 ctatcgatgc cgatatcgtc ttcaaggacg gcacctggca tatgttttac aaagggaata
     2641 ccaaagacga gcagggcaga gaattcgaaa acggcataaa acaggcaacg gcgcccagtt
     2701 tgcaaggccc ctggaccgaa catttcgaat acctcgatgt gtaccacaat acgcgcacca
     2761 atgtggaagg ttccagcgtg tttaaattga acgactccga cacttatatt ttaatgtacg
     2821 atctctattc gagcggaagg tacgagtttc agcgctctca ggatttgtat cgtttcaccg
     2881 aaaagcccga gtcgtttgtg aaagattttc atcccaggca cggcagcgtc atcagcatta
     2941 ccgggaagga agctgagcgt ctgaaccgaa aatggggcgg caccgcgctc gaataattct
     3001 atccccaaag ccgggttgtc ccggttgtat ttaataacat cgtgtgtatc cggtaatatc
     3061 atgagcaaaa aaatcaattt ccttgtcctt ctggcttttt taacagccca tgtctttgcg
     3121 caacacagtc tggaaaagct ggaccgtggt ctggtagccg tcaaaatatc caacggggtg
     3181 tttctgagtt ggagaatacc cggcgaagag tggcattata ccacctacaa cgtttaccgt
     3241 gatggaacca agctcaacaa cgaagctttg caggtttcca attacatcga tgcccagggc
     3301 agcctcagca gcacctatac cgtggcctct gtggtggatg gggtggagca ggagccttcg
     3361 gctccggttt ccgtttgggc acaggcttac aaagaattaa tactggatct gcctccgggc
     3421 ggggttaccc ccgataacgt aagttacagt tatactccca acgatatgag tgcggggcac
     3481 ctggacgacg acgacaggta cgatttggtg ataaaatggg agcccacaaa cgctaaagac
     3541 aattctcatt cgggttatac cggcaatgtt tatttagacg gatacactct ggacggcgac
     3601 aaactctggc gcatcgactt gggtgtcaac atacgggccg gagcgcatta tacccagttt
     3661 atggtgtacg atctggacgg cgacggtatt gccgaaattg cctgtaaaac ggctcccggc
     3721 accaaagacg gcctggggaa ctacctgagc aaggggcctg ccgccacggc aaaccacagt
     3781 gccgattacc gcaatagcgg aggctacata cttacaggcc ccgaatacct gacgattttt
     3841 gacggaagaa ccggagctga attgaccacg gtgtcgtacc atcctcccag gggcagtgtc
     3901 gatgcctggg gagacggcta cggcaaccgg gtggaccgtt ttctggccgc cgtggcttat
     3961 ctcgacggtc ggcgtcccag tctggtcatg gccagggggt attacacccg cacggtgctg
     4021 gcggcttacg actttgacgg taccaaactg acacaacgct gggtttttga ttcgaatact
     4081 tcgggtaacg gcggctatgc cggtcagggc aaccacaacc tgagcgtggc cgatgtggac
     4141 ggagacggaa aagacgaaat cgtttacgga tcctgtacca tcgacgacaa cggtaaaggt
     4201 ttgtacaata cctacctggg acacggcgac gccatgcatg tgagcgattt tgatccttac
     4261 cgcaaaggct tcgaagtctt taaatgcctc gagtcgggcg atggcggcac ggcgctgtat
     4321 gacgcggcaa acggcgatat tcttatcagg cataagacct acaacgattg cggccgttgt
     4381 atggccggta acgtgaccaa cgcctccccg ggagccgaag tctggggcgg gggcaagcga
     4441 ttctcggcca ctacccgcca ggatatcagc ggtctctcgc tgaatgtatc ggaaaatttc
     4501 agaatctact gggacggtga tttactgagc gaaacactca accataccac cgttaccaaa
     4561 cccaccaacg gaagtacgct gttgcaggcc tccggctgtg aatcgaacaa cggcaccaaa
     4621 ggcacccccg gtttgcaagc cgatttattc ggcgattggc gcgaagaact tgttttcagg
     4681 acaagcaatc cttccaaaat caggattttt actactaccg ctcccggcac ttaccgcaat
     4741 tataccctga tgcacgaccg gcaataccgt ttggccattg cctggcaaaa tgtggcttac
     4801 aaccagcccc ctcacccgag ttatttcctg ggcgaagccg agggtatagt ttcgcctccg
     4861 ccgcctgtaa tgaccaacgg acgcctggtg tacaagggag gcggtgtatg ggacaacact
     4921 tccgaagcct ggacgctcga cggacagaac actgtgtaca gtgacggccg gcacgtcttt
     4981 ttgaccggtc cccagggaac aaacgacagc attgagctct ccggaacctt gcagccttcg
     5041 gtgctgagta tggcttctcc gggtcgtttt tacctgaacg gcgaaaacgg aacattatcg
     5101 ggcaatatga aactgatcaa acagggcagc ggagtgttta gtctggacgg tacccacgat
     5161 ttcagcggcc cgaccgaagt ctgggacggg aagatgattt tttcgggcag ccttagcgcc
     5221 agcccgctgt ggttgaacat caacacagaa ctggctgctg caggacacct gaatcaggga
     5281 ctggaaatgc gttacggctc gaaattgttt gtcggtggcg agcaggcggc gggagagctc
     5341 aacatcaccg gaactttgcg tctcgaagaa aaagccgaac tggtctttga ttttacgggc
     5401 aatcagcccg gtgaggcaga tgtcataaaa ctaaacggcg acctctatat gcagaatgct
     5461 gccgtagttc gtattttacc tgcctttgcc gggggtgaac aattttgcga gggagaatat
     5521 accctgctta cttacaccgg caatgctcaa gttgatataa gtaaaataat tctggagggc
     5581 atacccgaga ttccggccgg cctggcattg gtaaacgata cgctcaaact cgtggtacgc
     5641 tcctcgcgtg agcccgcaac catcctgtgg aagggcagct attcgaacaa ttgggatttg
     5701 gtgcagacca aaaacttctg gtacaacgac agctcggact attttgtcac cggcgatacc
     5761 gtcattgtca acgatgaggc cggcaaccag gttctgaatg tgaccgaaac cgtaatgccc
     5821 tcggcgctga ttttcgaaaa caccggtaat tttgtgctca ccggcaaagg agagatagcc
     5881 ggcgagggca cattgaccaa aagaggcagc ggcaaactga gtatcttaaa taccaacagc
     5941 ttcacgggca aagtgttgct cgaagaaggc attgtggaag tgaaagcttt gcccaactcc
     6001 atcgacggca acggagctat cggcccggta tcggccaatg ccgccctgtt cgaaatcaac
     6061 ggaggaaccc tcggagtgac ggctgccacc acgtccgaca gagccctgac ggtaggttcg
     6121 gccggagcta ccctgcaaac caacgccaac gtcgcctgga atgcccgtat ttcgggtggt
     6181 cgcctcatca aaaccggcaa ccatacccta tccttgtcgg cagccaatac caataccgaa
     6241 ttactactca aagccggcac cgtacaactg agtaccgagc aggctcagcc cggacgtaaa
     6301 gtgatcttcg aaggaggtac gctcaaggct ttcgacagca gcggatccta cagttccgcc
     6361 agttatcccc tggtggtcga agaggggcag accggacgtt tttacgttga tagtcgctgt
     6421 acctattcca ataccctgac cgggggcgga accctctata tctacgtgcc ctgggtgcgt
     6481 tccgatttcg agggcaactg gagcaatttt accggcacca tagtgttgat gaatcccaat
     6541 gccttccgta atcaaagcac ctacggctat gctaaagcgg tgatagatct gggaaccgaa
     6601 ggcgtatttg acgatatgaa gagccagacc gtcaagatcg gctcactcaa aggcaagggc
     6661 aggttatggg gagccggtac ctgggaaatc ggttaccgta acgaagactt cacttttcag
     6721 ggtaatatcg aaaccggtgt catcaagaaa gtaggcgccg gcactatgac cctgagttct
     6781 gccatgactt ctacggccaa tttcctgatc aacgagggag ggctcactgt tacgggtctt
     6841 accagcggcc ccggtacctc gaatgtttat ataaaagacg gcgcttttat gagcggcaac
     6901 ggaaatattc agggaagcat aatagtggag gaaggcggca gcctgtacgc cggcctgtat
     6961 caacccaaca acctgacggc gggcacctct ttcagaaccg ccaacgtgca aatgcagacc
     7021 ggttcccatt tcgtggtcaa agtagatccg ggaatggaaa aatcggatat gatggttact
     7081 tcgggcaatt ttgtcgccag aggcaactta atcatgcaaa acatttcagg tcagccttat
     7141 cgggagggca tgtctttcca gatagtgagg ccgggcacca ttacgggagg ctttacatcc
     7201 gttgagccgg agagcccggg cgaaggtctg gaatgggatt tgaacgattt tacctcgacc
     7261 ggccgtatca acgtggtggc cgcctcagct ctcgacaagg cccgggccgc cgaacttttg
     7321 gtttatccca acccgacagc agggaagctt tttgttcaac tgccccgggc cgccggcgaa
     7381 tggcgtatta aactggaaaa tctcgacgga cagctcctga tggaacaagg tgcctacgac
     7441 agcagactgc tcgaaataga cctgactcat ttgaaacaag gcctttatat catcacggta
     7501 tattccggtg agcagtggat gaaacggaaa gtattgttga aataatgata catcgaaacc
     7561 ataccgccga gtgtaaatca agtacttgag aagcacttga gaagctctgg taaaaaaccg
     7621 aaaaacagaa aaataggaaa acagaaaaat aggaaactga aaaatagaaa aaccgaaaaa
     7681 acgattatta gttggaaatt ttaaaacata aagcaatgag aaatttttta ttgattgccc
     7741 tgatgctggg tttggcttcc tgtggtttac aaaagaataa gcctttgact gagaaggata
     7801 tgtctgctta tttgctggtg tattttctgg acaataccca cagtttgcat atggccctga
     7861 gcgaagacgg atataccttt acagccgtca acgagggcaa agccgtggta gccggcgata
     7921 ccatagccgg tcagaagggg atacgcgatc cgcatattac ccgtggcccg gacggtgctt
     7981 tttacctggc tatgaccgat ttgcatattt tcggcagaca gcggggttat cgcgacaccc
     8041 agtgggagag gcctcaggaa gaatacgact ggggcaacaa ccggggtttt gtattgatga
     8101 aatcctttga tctgatccat tggacacaca acaaggtgta catccaggaa gcctttcccc
     8161 atttggatgt gggctgtgcc tgggctccgc aatgcattta cgatccgcag gaggaaaaga
     8221 tgatgcttta ctttaccatg cggctgggac acggcaagac ccaattgtat tatgcttata
     8281 ccgacgatga ttttacccgc ctgctcacgg agcctcagtt gctttttgag tatcccaatc
     8341 cggctgttca gattttggat gccgatatta cacccatgcc cgacggccgt tactgcatga
     8401 tgtacgtggc tcaggaaaat cccggaggca ttaaaatggc tgtttccgac aagatcaaca
     8461 gcggctatgt gtacgatgaa cgctgggtcg atcttgagcc cggcgcttgc gaagccccga
     8521 atgtgtggaa aaggataggc cgggacaaat gggtgttgat gtacgatatt ttcagcatcc
     8581 ggccccataa tttcggtttc gtggaaacct ccgattttga gcattttgag tccctgggtc
     8641 gttttaacga agccgacatg aaaacgacca acttcagctc tcccaaacac ggggctgtta
     8701 ttcaactgac caaaaaagaa gcaaaaacac tgagtgatta ctggaaatga ttcacacatg
     8761 aatgcccaaa gctgcaccgc ctgttttatt ttgaccggta cagataaaat gatgaaccct
     8821 atatgatcct atacttactg ctcgttgttg ccggttttgt ttgtttaatt tacggtgcga
     8881 attggttggt cgatggtgcc tctgctttag cgaagaaata taaaatgtcc gatttggcca
     8941 ttgggctcac cattgtggct ttcggcactt cggcgcctga attggtggtc aacgtggtgg
     9001 cttccatcaa cggccattcc gaccttactt tgggaaatgt aataggaagt aataatttca
     9061 atactttcat cattctcggt ttgtccggcc tgattatgcc cattgcggta cagtcttcca
     9121 ctgcatacaa agaaattccc atgtctttgt ttatcacggt attactcttg tttatgctga
     9181 acgatttcgg gttttcaggc gagggatctt taagccgttt tgatgccatt gttttattcg
     9241 cgctgttttt gctctttatg tattatgtgt ttaaccaaat gaaaagcgat tcagccccgg
     9301 ccaccgccgt ggtggtaaaa tcgaacgtaa ccatagggag tttgatactg cttggattgg
     9361 tcgggctggc agcagggggg catttggtag taaagaatag tgtcgcgatt gcgaacgctt
     9421 tgggtgtcag tgaaaaaatc atcggattga ccatagtggc cgccggaact tctttgccgg
     9481 aattggcgac ttcggtggtg gctgccataa aaaagaattc cgatatcgcc attggaaata
     9541 ttattggctc caacatcttc aatatatcgt tgattttatc tgtaagtgcc tttatcaggc
     9601 cgattgcata taatccaaaa ttcaatcagg acttgttggt gctgattgga ggtaccgttt
     9661 tcctgctgat ggccgtacta atcgggaaaa gaaaacaact gagccgttgg gaggctgctg
     9721 ttttgtttgt ctcttacgca tcgtacatgc tttatttggt gatgcaggag ctttgattat
     9781 tgtcttgttt tttgaaaaac agacgggttt gcattcattt tataaccatt atgaaaagat
     9841 tatttttgac aggatgcgtt ttatcgcttg gtttctgttg tgtttttgcc cagcctttgg
     9901 cttttcccgg agcagagggc tttggtgcca attcccgcgg aggtcgcgga gggaaagtga
     9961 tttatgtgtc caatctgaac gacgagggac cgggcagcct gagggaagcc gttgaacagg
    10021 aaggcccgcg tacggtcgtg ttttccgttt cgggcattat cgatttgaaa acgagactca
    10081 acatagagca tccctatatc accatagcgg ggcaaaccgc tccgggagac ggaatttgtt
    10141 tacgcggcga aacccttcgc atagcagccg atcatgtcat cgttcgttac ctccgggtca
    10201 ggctgggcga cgctaagcac ggacagggca gtttacaggg caaagatgct atcagtatca
    10261 gttccggtcg gcacattatc ttggatcatt gttctgccgg ctggagcctg gacgaggttt
    10321 tatcttcttc gaccagaacg cccgatttga gtaatgtcac tgttcaatgg tgttttatca
    10381 cagaaggtct gaatcccgac ggacatggtt ttggctctct tatccggggt acgggaggag
    10441 ccagatacag tttcctgcat aacctatacg ctcacaacag ggggcgcaat ccccggccgg
    10501 gcaattacga ttccaatccg cacgataagg accccctggg cctcctgctt gatttcagaa
    10561 acaatgtggt ttacaactgg ggtggagaac atgccgctta caatgccgac aaagtgagtg
    10621 taacccgctt gaattttgta aataattact tcgttccggg gcaggactcc aaagcaagcg
    10681 gcattgtcta ttctaccggc tcaccttata atcgtgccta ttttgccggg aattactaca
    10741 gggatgagtt gcccgatgat ccatggaagt tggtgaaatt caacgaaaac tggagtcaga
    10801 aagatatctc tgcctacaaa caaaatcagg ccttcgagtc cggccttgtt caaaccgatg
    10861 aggctcccgt agcctacgaa aaagtgctcg aaacaggcgg agccgtactt cctaaacggg
    10921 atgcggcaga tcagcgcata gtaaaggacg tgcgtcggcg tacgggccgg attattcaaa
    10981 gccaggaaga ggtaggcgga tggcctgaat taaaaagcgc cgaagctcct ctcgataccg
    11041 atttagacgg catgcccgac gaatgggaga agaaacacgg tctaaatccc aaggaccccg
    11101 aagaccggaa taagctgaat gccgaaggct acaccatgct ggaacaatat ttgaatagta
    11161 ttggaagttt tcagccggtt gcttccggga aacacagagt aatcgtattg accgacatcg
    11221 aaaacgaacc cgacgacgct atgtcgctgg tgcgcttttt atgctattgc aatcagtggg
    11281 atgtagaggg tttggtggcc accacttcgg tccatcaaca aaaagaaacg gccgcctggc
    11341 gcatcaaaga aatactgcag gcttacggcc gggtcaggga caatctggaa aaacacgagc
    11401 cgggttttcc cacggcagat tatttattgt ctgtgctcag ggaaggaagg cccgactacg
    11461 gcatgagggc cgttggtaag ggcatggatt cgccgggttc ggaattgatt attcaagcgg
    11521 tggatcgtcc cgattcaagg ccggtctggg ttttggtctg gggaggcccc aactgtctgg
    11581 cacaggcctt gtggaaagtg aaagaaaccc gctcaccaaa agaactggaa cactttgttt
    11641 caaaactcag agtatatact atttccgacc aggacgacag cggcccctgg atacgcaaga
    11701 cttttccgga actgttttat attgccagtc ccggttttca ccgctacggg gggtatcatt
    11761 acgccacctg gagcggaatc agcggagatt attttcatgc tcgttgcgcc ggcggcgatt
    11821 tcagtctggt tgataacgat tgggtggaca agaatgttca aaacaagggc cccctgggag
    11881 agcaataccc cgatattaaa tttttaatgg aaggcgattc tccaagcttt atgttcttga
    11941 tagataatgg gctgggcgat ccggagcatc ccgattgggg gagttgggga ggccgctacg
    12001 agtattatct gcccaaattt gagaaatggt ttctggagcc cgaaacccgg ccgctgtgga
    12061 ccaacgccat ggatgaggtg ctgggcagcg acggccgttg gcacaccggc aatcacgcca
    12121 ccatctggcg ttggagagaa cattttcaga acgatttttc ggctcgtatg gattggacaa
    12181 tcaaaaccta tgagcaggca aatcatcctc cggttcccaa actcgggcac gaagcctacc
    12241 tccgggcaaa agcgggcgag cgggtaaatt taagcgcagc gggcagttcc gatcccgatg
    12301 gcgacagcct caggtacaac tggtttatgt acggagaacc cggcaccttt gccatgggca
    12361 cagccagaac aggcaatcca ttgaaaataa acgattccga caagcaggaa gcctggttta
    12421 ttgctcccga aaaagcccgt atgggcacca tgcacatcat actggctgtc agcgacgacg
    12481 gtcatccctc gctgaccagg tataagaggg taattgttga gatacaatag tttaaatacc
    12541 ctgtttgcag gtagctaccg gaaaatggct gcggtgatga aaatggtcgg aaaaacccta
    12601 tggaatataa ttggcctcac ggtggccctg ttgttgctgt ttttgatatt tatgattatt
    12661 ccacaattcg gtaaggcccc ttcgggcagg cgtcaggccg ggatagaggc ttcggtgcat
    12721 tataagaacg gacgttttca caatttggaa cacacgccca tgtttgtgga acaaagcgga
    12781 aacaaaagga tgtttcctaa attccttttt cgaaacaaga aggacttgcg acctactttc
    12841 cgaatacctt cggtagctac caggctcaaa gaccttccct ccgggcaaaa cctcctagtg
    12901 tggtttgggc attcgtctta ttatttccag ttggagggaa agcgttttct ggtcgatccg
    12961 gtattgggta gaagagcctc tcccgtgccg ggtatgctca gggcttttcg cggaactgtg
    13021 gtgttcaggg tggaagaatt gcctcccata gactatttgc tgattacgca cgatcattac
    13081 gatcacctgg attacaggac cataaaaaaa ctcagacgca aagtgggggt ggttgtttgc
    13141 ggtttggggg tgggttcgca tttgtcgtat tggggctatc cggcctccaa aataatcgaa
    13201 aaagattgga acgaaagtct gatgctcgac gataagatca ggctgtacac cgaaacttcg
    13261 aggcattttt cgggtcgttt cttatcgaga aacaatacac tctggctgtc ttatgtcatt
    13321 aagacgcctg gctacagcct gtttatcgga ggcgacggag gttacggcag ccatgtggca
    13381 gagattggaa aaaaacacgg ccctttcgat ctggctgttt tggaaaacgg gcagtacaat
    13441 gctttgtgga ggaatatcca tcatttcccc gaagacgttc tgcggtctgc ccgtgacctt
    13501 agcgcccgcc gggtacttcc ggtgcattcc ggcaaattca gtttggcttt tcatccctgg
    13561 gacgagccgt taacacgtct gagcgaattg aaccggcaat cgcccgagcc tgttccattg
    13621 gtgactccac ttattggcca agtcgtcgag ctcgacaacg aaaatcaagt tttttcagaa
    13681 tggtggctga agccgtaaaa attgtatttt tgttgccaaa tcccttaaaa tatagttgtt
    13741 tgtttcctgt tatttaattc aaattcacca gatgaaaaca aggatatcct ttttaggcaa
    13801 gtatgcaagc ctgctcttgc tgctttcgct tattttgtcg accggttgct ctttgggcag
    13861 cagaatctac aaaaaatacg accaggttca aaaagtagat cgttacaaac tggacttgaa
    13921 ttacccggat tttaacctga ggtcaccctt gttgtatatg gagcaatcca ttgtaaaaga
    13981 ggtctctgca aaggagagta aatacagcgt ttacgatgtc ttggttttga acagtgccag
    14041 ttacagagtg aaagaggagc tttacatcat cgtagaagac gagattttta agcctgctgt
    14101 taccggcctc gaatacgata atgccaggga gatacgggag aaaagcgaag aggtggtgag
    14161 agccgattct tcgacggtaa aagtggtaac cggctatacc gagaacaata aaaaaattac
    14221 ccgtttcact tatacactcg ataacgacgt aattgagcgc atccgtaaag ccagcatcgt
    14281 cttgtttcgc tattatgccg gcccggacat ggtaacaatc aagctgaaag gcctcaaatt
    14341 gaagagagtt aaaagattga tcgaaaaata gcgagtaatg ctgaaaccgg acagtaggac
    14401 gtaattatgg caaatcattc acatcatcgg catggacatt ccctgcatca cacgcatatc
    14461 tggtcgctcg acggggaacg gcacgtgttt actacccacg tgaaattgga aaattagaga
    14521 gtttcgaaca acttctgaaa gtaaaagaag aaattaagtc cattctggag gactacagtt
    14581 ttgaacacta tactgtcgag acggaattgg atgaagaaag cagctacgtc tcttttgata
    14641 aatcactcta ttacggtcgt acatcaattt ccgataaaaa tttaataaca ttttattatt
    14701 gttgtaacag ttttcttcat gggcttagct aactttgcac agtttgtaat tagaatgtcg
    14761 aactaaaaca aattgaacta tgaatgaaaa catatttaac aaaaaaggga ataatacacg
    14821 tagcttggtg gttgtacttg gtgcggcttt ggtattaacg ggtctgacat tacttgcatt
    14881 taattttggt tggttaaatc ccgcattcaa atcggttatc ttttcttggc ccatgctttt
    14941 tatcctgttt gcagttgtcg ggttttctaa aagacaaata ctacttcctg tccttttttt
    15001 attgatcgga gtttttttcc ttttgccacg tctggagatt gtttatcccg ggatattagg
    15061 cgaagcaggt agaggaggtt ttgccaaaaa tttttggccg tttttgctga taattatcgg
    15121 tttgatgttt attgtcgggg ttgcgaccaa aagaaagaaa aaagtttctt tcaagcaaga
    15181 tgtcgttgat acgcaggctg ttacatctgg agaaagtggc tggataataa gcgatgttgt
    15241 ttttggaggt ttggaaagcg tttttctgga acctattctt aaaggcggag atattgatgt
    15301 ggtttttgga ggaatggtga tcgatttacg gaaaaccact ttgcccgata caaccgtgta
    15361 tctcaatgtg gatgcaattt ttggaggtat aacactttat attcctgaag attggtgtgt
    15421 aaaatcaaag ttcaatcccg tctttggagg ctacagcgat aagcgagcga gtataattgt
    15481 agatgacaaa agcgacagtc ggcttgtttt gcaaggttct ttaatttttt ccggttgcac
    15541 tgttcagtag ttttattcaa tgaaaaagat gatgcttggc ataaaaaaaa tatggatgtt
    15601 atcgcttctt atgtttttgt cggtatcggt aagtgttgca caaaacgatt tagcggaaac
    15661 agtaagaggc gtagtggttt cgggcgacca tactcatgag ttgattcagg cgtcaatata
    15721 tgtcgagggg agcgaacccg gaataggaac ggtgacggat gaacaaggta actttacttt
    15781 cagggttcct gtcggaaggc aaaagttaag attcagtagt gtggggtttt tatcacaaga
    15841 agttgatata ctggtaaata caggtaagga ggttgtgcta aatgtagtgc tggaaccttc
    15901 ctttattgaa ttacaaggcg cagagatagt ggcaagacat gataaaagcc ggccaatgaa
    15961 caagttgtcc gttaccggag caagaacttt tagcacggaa gagacctttc gatttgccgg
    16021 ttcgctcggt gatccggcac gtatggtgcg tagctttgca ggagtgattc ccgcaaacga
    16081 ttcgcgaaat gatattataa tcaggggtaa ctctcctgta ggtgttcaat ggtcgttgga
    16141 tggcgtagag attgctaatc taaatcactt caataccgga ataggcatga caggtggaca
    16201 agtcaccttg ctcaatacca acttgctctc caattcggat tttcatatga gtgcctggcc
    16261 tgcctcctat ggcaacgccc tggcaggcat attcgatttg aaaatgagaa agggaaacaa
    16321 taagaaacat gaattttggg cacagatggg ttggaatggt tttgaactgg gttctgaggg
    16381 ttatttttca aagaaaagca cttcatctta ccttgtgtcg tatagatatt ccatcccgga
    16441 cttgatggat aagatgggtt tttacaaggg tataacacct aaataccaag accttactat
    16501 gaagctcgat ttcgatttaa atgagaaaaa tcatctttcc ataatcggat tatggggcac
    16561 tagcgggatt gggtttgtta tgtctgaagt aacctcggtt gatatcaatg aagacttctt
    16621 gcaaacaacg gatcaacgaa tagctataga tgccaaaaca ttgatagccg gagctacaca
    16681 cagcgttgaa tttacaccga aaacaaagct cactactctt ttctctttcg taagaagcga
    16741 cacccatatg ccggtagata caatatctct tatacagcct aattcagagt ggcaaacgct
    16801 ttgggatgaa aatgcactgg aagataaata ttcgctccac agcaaactgg agcatcgatt
    16861 ttcctacaat tcgctaatag aggcgggagc gaagtacgat ctctataatg tgaaatatct
    16921 tgaaaaagag tcattcagcg atgagagagg cttttttacc aatgttgatg agaagggtta
    16981 ttttagtctc gcgcgggcct atgctcaata tagacataat ataacatcga aattgttgct
    17041 gacaggcgga gctcatggga tgtatcttca attaaacaaa acgtatgcag ttgagccaag
    17101 gttcggtctc cgctacacac ccgcatcaaa acacactctc gcattggcag gtggactata
    17161 cagccagatg attccacgct cattctattt tatcagaact ctcaatcctg aaggcgatat
    17221 tgaatatagc aacaagcagc ttggatttat gaagagtgcg catggtgacc tttcgtacga
    17281 ttgggccttt gctcccgatt ggcatttcaa agtggaaact tattatcaat ggctttatga
    17341 tatacctgta aaaaataacc ctgacgcaac ttatacaatg ttgcagatag ggggtgctgg
    17401 cgataacgtg attatgagag aggggaatct tgtaaacaaa ggaacgggaa aaaattatgg
    17461 tgcggaattt accattgaaa agtttatgag taataattac tatctcctgt ttaattccac
    17521 tatttacaga tcgacctata caaacggatt taataaaaag gagtggagta cgatttttga
    17581 cggtagatat ctctttaatc ttgcgtcggg ttacgagctg ccgctcaaaa aagggtggac
    17641 actctttgcc gatataaagg gctcattggc aggcggtacc cgctacactc ccgtgttgga
    17701 agatcgaagt aggttggagc acagggtggt gtacgacaat acacgcatta atgaattaca
    17761 agtacgcaac tatttcagaa ttgacttgcg aatgggttac aggaaaaact ggaagaggtt
    17821 aacagacgaa ttgtttgttg atttccaaaa cctgacaaat agaaaaaacc tttatggtct
    17881 cttttatgat attaatacgg gcacttatga ggaaatgttg ctacagggct ttttcccaat
    17941 ggtaacatat agaatcaatt tttctatagg caggaagaaa tgatcatcga atgggtatta
    18001 tgcccggttg tgtgtttaga ctctattttt tttaatacaa tcaaatagta cttgaaaacg
    18061 aaatcaattt tatcgggact cttttggatt gcggtttttt atatctgttg gggagtttct
    18121 caactgataa gtattaaaac gcagcactcg ctactgtcaa gcctgttgtt ttccattgtt
    18181 tttacaggac taatcgggtg ttttatccca atatatttta agaataaatt tcattggcgc
    18241 tacaataaat caggtcctga cagagtagtg ggttatcttt ttttgatctt agccattctg
    18301 ttttcaacca tattatcagg agcctttctt aaagtcattg aactgaaata ttcttggacc
    18361 ttaatactga aatatgtact gttgtttttc ccgatgtcat tgggcatagg cttatttgct
    18421 tttttgttga ttccgaacac gatacagggt tgggaggaga acaaaacgaa aagcgttctg
    18481 ctaattattg caatagtcgc tttcttcttt ctctcgtttt atgttgattc gctgtttcaa
    18541 gatatggagt tggctgttac tatgggcttt atcggattgt tgctagggct aagctatctt
    18601 tatatcagaa atttttggat tgtgtacccg gcacttttta taatcatgct agtgaacaca
    18661 ttggctgaca acaagtacga tgaatacaat tattgggttg taattgtcag cactctgttg
    18721 tctttgacaa tactcacgtt tgactttata aaaaatagaa aaatcaataa ttgaaacgat
    18781 acaatctgac ggttggcaat ttgcaataat gacaaccatt ttaggcggac tacttggatt
    18841 ttcaattaat aaaacattga ctaaaaaacc gaatagaaag aaataattgc atcaaataaa
    18901 aaatatttgg aaacgatcaa acccttcgtt tcagatttaa gagagaaatc cgacgtgtca
    18961 tttattgaga atgcaacgaa atcggagaga aactcaacaa aaaagaaagc agaacaatta
    19021 ttgaaaaaag gattgggtat cggatgaaat tttacaatag aagcggaaaa atacaaagtc
    19081 aaaataaatc atgaaacgat tcattcgtta cccatggaat atccggaaga actaaacagc
    19141 gagatcatac agtttattga aaagaatagt aattattaaa tggaaaataa aattattgca
    19201 aagtacaaca gacctgtttt cttttatggt ctatcgttgt taatcccgtg gattctttgg
    19261 tttacagttg cctatatcag tcatttgccg ggacaaagca gttttttaat ttttattcag
    19321 gcgctgttag caatacttgg attattggca ccgacttttg tggctgccta tttattctta
    19381 tcagacaaag agttgttcaa tgacctgaaa aaaagattta tccgtcaaaa aggctttagt
    19441 ccaatatata ctatattagc tttttccttg atttttgtat caattgttat agcccagctc
    19501 atatcattga tatttggaca tagcattgac caattttaca tttccggatc gccaagtttt
    19561 acctcggcat tactttcacc ttggttcatt ctgctttttg cgccggtagt ggaagaattg
    19621 gcatggcact cgtacggaac tgatgcccta agacagaggt ttaatctttt tacgacttcc
    19681 ataatttttg ctctatactg ggctttttgg catttgccgc tttcctttat agacggttac
    19741 tatcacagta atgtagtaga agaaggtttg ttgtattctt tgaattatgt attcagtctt
    19801 tttgttctga tttttttaat gaattggctg tattacaaaa caaaccggaa tatttttata
    19861 acaatagtat ttcattgttc agcgaatgta tcaaatgaga tatttgccac tcatcccgac
    19921 agcaaagtaa ttcaaacagt attgctactt atcgttgcta ttgttgtttt gtttaaagaa
    19981 aaagggatgt tttttagaaa agagtatcaa caactataga ttttcttagg cacatgaatg
    20041 ggactaaaaa agatgtgctg cgatttcttt tgcttgtcat tccgctatcg gtaccgtttt
    20101 atttgttgaa tctaattccg gtacaattgt ttccttttgg aatgcctacg agtgtgctta
    20161 cgataattgt cccgtttgga ttagcagttt atggtgtcgg gaaaaacggt tgttgtgtat
    20221 tacttagttt atctctgtca aaagcaaaca atatgctcga tttaaataat aagaaatgag
    20281 aaagtatttt acagcaatta tatttgtttt gttttgtgta aattcttatt ctcaaaattt
    20341 taccaacaat gtaaaaggcg tgattaaaga tgcttttaca gatgaaccat taataagtgc
    20401 cactatcact cttaccgtcg atacattaaa atttcataca atttccgata acgagggaaa
    20461 atttcatttt gaaaaggtta atgcgggcag aattagtttg aaaatcaatt atttaggtta
    20521 tgaaccctat tttgcaaata atttgatact ggcaagtgga aaagaacttg aggttgaagt
    20581 acaaatggta cagtcaactc agagcttggc agaagtaaca atttttgcaa gggagagagg
    20641 cgaattaaac aatgaattgg cttatgttag ttcacgctct tttgatgtga aagaaacgga
    20701 acgatatgcc ggaaccagcg gcgaccctgc aagaatggca acttattttg ccggtgttca
    20761 ggctgcaggt gattcacgta atgatataat aatcagagga aattcgccgc ttggattatt
    20821 atatatgctg gaaggaattc ccattcccaa tcctaaccat tttgccacta tgggaacgaa
    20881 cggaggcgca atttcgatgc tgaacaacaa tcaattatca aactccgatt ttttgacagg
    20941 tgcatttcct gctcaatatg gtaacgccac cgcaggtgca tttgatttag ccatgcgaaa
    21001 agggaacagc gaaaaatttg aatatttact tcaaatggga acgggaggtt tagaagcagg
    21061 tgttgaagga ccattttcca aaaacagaac ctcaagtttt atggtgaatt atcgttatgc
    21121 ctttttagaa ctcttcgata aaattggagt taatttcggc actccctcta tacctaaata
    21181 tcaggattta acattcaagt ctgattttga tttcgggaaa acttctctgt cgttgtgggg
    21241 atttggcggt ttttgttcca tgtcgatgga tcccggtaaa aacgtcttga attttacaaa
    21301 aaatctgaga acagaaatgg gtggtcagac caatgtggca gcaattacta tgaaaaacat
    21361 tattaattca aaatcaaact tgcgaacttc attggctttc acaaattcca ggtcaagcat
    21421 gtctgttgat agtgttttca aaaacagtag agaattccat ttctttggtg gcgtttatac
    21481 tgaaaacaaa ttaattgcaa caactcaata taaaaatcgt ttcaatccca aaaacagaat
    21541 aattgtgggg gcatcttaca ctcattcatt tgtaaatttt gccgatagtg caaaaatcca
    21601 aaatcaatac gtttacggca ccaaaaacag aggaaactat ggacttgctc aatttaattc
    21661 tcaacttcta cacagtatta atgatagagt tgaattgact atgggtgtgt ttaatcagta
    21721 ttcgctatta aacaactcgt tgtctgttga accgcgggca gccattagat attcgtccgg
    21781 tcaaaatagt gatattagct ttgcctatgg gttacactct caaactcaac ccggaaacgt
    21841 ttactttaac caaactttga cagacactat aaatcaaaca tataaactga ccaatgccaa
    21901 tttggatttt tctaaaagtc accattttgt gttgggatat aatgtgaatt tcagaagaaa
    21961 tttcaatttg aaaaccgaag tttattacca aaacttgttc aatataccgg tagaaataaa
    22021 aagctcacat tattcggtga taaattatgg tgccgatttt tatgaagtag taaacgacag
    22081 tttggtaaac aagggcaaag gaagaaatta cggtattgaa ctgacattag agaagtattt
    22141 ttccgacaat tattattttt tgataacaac gtcattgttc aattcaaact atttagcatc
    22201 agataatatt tggcgcaaca ctgtttataa cagcaatttt gttttcaatg cccttggcgg
    22261 ttacgaaata aaattgccaa aacagcaaac attgacgata aattcgaatg tggtttatgc
    22321 tggtggcctg cgtaatatcc cgatagattt agagaaatca aaaatggatg gttacacggt
    22381 ttatgattac aaaaatgctt ataaacataa gaatcccgat tttttcaaag caaatattaa
    22441 attgctttat cgtgtcaatt tgaaaagatg gagttatgaa tgcggtttcg aactcacaaa
    22501 tattacaaac agaaaaaata tttggcggca aatttacgat gcggaaacac aaaccataaa
    22561 aactgattac caaatgggta ttatgccggg agggctgttt aggatatact tataaacaaa
    22621 gtcaaaaaac aagttcagaa aacccttgtt tcagtactcc tcaaaaggta tcaacaactg
    22681 tgggttaagc gtggtttcgg agagaatgtc ggcaggtgga ttacttaccg aaaagccgat
    22741 taaccggacg ccgtgattat ctaaatttac cgtttccaaa agttctgttg ccaactcgat
    22801 aaattccctg gccaatgtaa aacaagcttc tgccgtttga cttcgggtga cttgcttgaa
    22861 atcgccccgt tttattttca gtgtaaatgt atatccttga aaatgagctt tctcaatgcg
    22921 tctttccagt tcataggcca gatcgtcaaa gcgagcgatg atctgctctt ttcccataat
    22981 gtcctcgtca aaagtatgct cgcatcccac ggacaggcgt tttctatctg gttctacttc
    23041 gcggtggtcg ttgccctgcg caaattccgc aaaaagtttt ccgattttcc cgaagttcct
    23101 gaccagatag tcttccggtt gttcccgcaa ctctttccct gtatggatac ccagctcgtg
    23161 cattcgccct gccgtaacct ttcctacgcc ccaaaaagct tcaatcggca ggttttcaat
    23221 tactcttagg gcttttttag ggtgtataac ggtcaaaccg tcgggtttct gcatgtcgga
    23281 agctattttg gccaaaaact tgttgtacga aactccggca gaggctgtga ggccaagttg
    23341 tgattttatt tttattttaa tctccttggc aatatcttgg gcgagaggaa taccgggctt
    23401 attatcagta acgtctaaaa aagcttcgtc aagcgaaagc ggttcaatca agtcggtata
    23461 ttcgctgaaa atatcgtgga tttctctaga caccgatcta tagacctcaa accgaggcgg
    23521 tacaaaaatc aagtgcggac aaagttgcaa ggcttttttt gaaggcatgg cggagtgaat
    23581 gccaaacttg cgagcttcat aacttgccgc cgcaacgact ccgcgagggt gcgaactgcc
    23641 taccacaagc ggttttcccc gccactcagg gtggtcgcgt tgctctacag atgcataaaa
    23701 agcatccatg tccacgtgga ttattttacg atatttcggt gtactctgca tatgggcaag
    23761 tcaaattata ccaattttcc gacaaaatct tcaatcaacc gattgacaac ctcaggcttg
    23821 tcggtatttg cattatgacc ggcatctttt atccattcaa taggaattcc tgtgtcctta
    23881 tgccatgctt tattaaatct aatggttgaa cctgcttggt ctttttcgcc acaaatcaat
    23941 agggtggggc agttcatctt ataaggcaaa tctttttcgt atgcttcggc aagaatttta
    24001 aaaccatgac ctgccagttt ggcgtatcgc tttttatccc catcgtaaac catcataatc
    24061 tcacgaatca gttttctgcc gtattcactt tctgcagttc cctttgatcc ggctttcaac
    24121 aaggatctcc atgggtacaa tagatataca ggttccattc ttttcagcaa ccaaagttct
    24181 atgccggtca aatattttct ttgcaatggg gcagaatcaa tggaaataaa gccttttaat
    24241 ttttccgggt ataattccgc aaaagtttgt cctacgtaac cacccataga ttgtccgaca
    24301 attacaggac atgtgatttt ttcgtgctca agtatttcat tcaaccattt cgccttatcc
    24361 atcaaattga aatcgaactt gaatggccag gaagaggcat gggctggcgg gtcccaaaca
    24421 aagacattgt atttgtcctt aaagtattca atttgctttt caaacagtct gtggtctgcc
    24481 gttaggcctg ggagaaaaac cagggttaca gcatcaacgt taatcttgtt tgtccaataa
    24541 tggataatac cggaggctgt ttcgtatgtt ttttctgtca ttttaccggt tttatttttt
    24601 cttcaaattt attttttttg tacacaactc ccagtttatc gcctttcact acctttcttc
    24661 aggccctaag actcgtgtct tctctttcgg taggaaagta cgcccaatat gccgacagtc
    24721 acgaaggcta ggacgatata gataaagaca gtggaaacgc catccgttac tccgtaggaa
    24781 tgcatgccgc tcagcaggta gttgacaccg aaaaaggtca tcaaaaccga tgcaaaagcc
    24841 agtaccgaga gcaggttgaa aagccacaga ttatctctct ttttcaccag atggatatgc
    24901 gttacgatgc tatagactat catggttata agcgcccagg tctctttagg atcccagccc
    24961 cagtagcgtc cccatgactc atttgcccag atagctccaa ggaaggtgcc gatggtcatc
    25021 agcgtaagac ccaccagcag agccatattg tttgtgatgc tcagctcttt tatacggaag
    25081 cgtatcagtt cgctcttttt tgcaaaggcc gtcagcgaca gattgacaat gcccaacagg
    25141 aacccgatgc cgaaaaaccc gtaagccgcc acgataaccg ctacgtgaaa catgagccag
    25201 ggtgacttaa gcacaggcac cagggtccct atttgcgggt ccatccagtt cagccccgaa
    25261 acaaacagaa tgatccctcc gaagagtgtt gccaatgcca tggtaatggt acttttcctg
    25321 ccaaaaacaa cacccgccaa aacggttgcc caagccacat ataccatcgt ttcataggag
    25381 ttgctccacg gagcgtaacc gccgatatac caacgcatcc ccataccaaa agcatgaatc
    25441 aggaaagcac ataaaacagc gactgataag acttttatgg ttgtatccgc ccatttttgt
    25501 tttttaagaa gctgcataaa ggccaaaaca agcatcaaac cgcccagaat aaagtaggac
    25561 agcttgcagc ggtcgaaaat tcgcatcctg ttgtatctga tctcagcttg tatctttcct
    25621 acatcgatat gtgctccgat gtcgtttttc aattgaaact ctcttatttc ggcaaccagt
    25681 tgctcgggtt tgctccactc tccgctttgc agcgagcggc tgacttcggc aagatagttc
    25741 ctgaaacagt cagcaacgaa cagagaatcc tcttttggga agatggaggg actgacgttc
    25801 ggcggatacc aggtgtgcgt ctcatcgccc gggtcaggat aaatccgcag caagctgtaa
    25861 tccaacagct gaaagacaat gttcaccctt tcatcaagtt tcatcatatc tttatcggtt
    25921 gccgagcgct ctgcaggtaa tttcccgtaa atcagttgca tctgccgaag caatttatag
    25981 ttgccgtttc cgtcgaaaaa ctgtgcgtaa gaggcccaat ctctgggcaa cccgtagagc
    26041 gatgagattt cgcggttatc tattgcaatt atcggctcat gaatccacag ttgcggctct
    26101 gcaaggaagc tgagcagaaa ctgctcaggg ttgaattgcc ccatctttag ctccttgtac
    26161 actttccgca ctatttcgga tgagagggtg tgcatcggta tgatccttcc gtccgattgt
    26221 acgggcaatg ctgcaaattg ggcggcatgt tccggcggga tcaagagatt cggggtgttc
    26281 tctgcggctt gcgcccgtga aacagttgct atgatcagca atatcaaagc gagggtaaga
    26341 tgtttgctct cttcccgcaa actcttaagc tgacggccca accggcgaag acgggagttt
    26401 ttcgtaaaaa acgtaagtaa aaatccgcag aaaagtaaaa agtaacctgt gtaggttatg
    26461 gtcctgcctg ccacatcgcg gtttacggag agaattgtcc cgagctcatc ccggtcgtac
    26521 gaagcctgga aaaaccgata gcctttcacg tccagcacgt tgttcataaa gacttttgcc
    26581 tcctgtactc tgccgtcaac atgtaccagc aggtcgcttt cataagatga ggggcttgcc
    26641 gaaccgggat agcggtttaa ccggaagtcg gtcagttcca acatgaacgg cagcttttca
    26701 acgctgatcc cttcggcagt gcggataatc atctcatcgg ttttactccc ttcccgaatg
    26761 tgtactgtgc cctctttccc gaaaaggaaa gttgtcaagg ccccgcccaa aatcacaaca
    26821 aaagccagat gcacggtcaa cagcgcccat cgcttctgtt ttacgtagcc ctgtctgctc
    26881 caggcggcaa tgaaattgag taccagcaaa aactgcaaca ggaaaaaagc gggcgaataa
    26941 tagacccact tttttgcaac atcggttcca tgatcttttt caataaatgt cgcgacagcc
    27001 attaccgctg catatatcag aagcagggtg atggttgtct tgtatgagga gagataacgt
    27061 tttgcttttt ccatagatgc ttcaaatttc ttcatttttt gtgtcaagac aaaaaaacga
    27121 agaaaaaaag tcaaggcagc tctcgtttta cacgaaaaac taccggttgt accgatgtcg
    27181 ctcctgctac aaggaggggc tgattgctat accgatacct ctgcaactac gaggatctgg
    27241 cggtcgtgcc gatgcttagg aaagttaaat aatattaatc tcttcgaggt tgtaaaaatc
    27301 tattttatat ttgctgcgta ttagtacact aatacaccaa taaacaatta aatattatat
    27361 taatatgatt aaaatcaggg atttaagatt tagttacggc aataatgtcg tacttaagga
    27421 catttcgatg gagcttcagg agggccggat atatggtctt ctcggagaga acggagtggg
    27481 caagaccact ctgcttaccc tgcttgcggg cctgaaggag cccggcgccg gttcaatcga
    27541 gatagatggt caaaaaccgt ttgaccgcac gccttctttt ctttcgcaaa tctattattt
    27601 gcccgatgag gttcctgcaa tccgcatgaa agctatcgac tttgcaatgg accggggaca
    27661 gttttgggtc aattttgacc cggacaagtt caaggaaatt gccggggtgt tggaaaccga
    27721 tacatccatg cgcatggatc agatgtcgta cggccagctc aggaagacct acattagctt
    27781 tgcgcttgcc tgcaacaccc gatacctctt tatggatgag ccgaccaacg gactggatat
    27841 accctccaag tcacagtttc gcaaggcgat gtccaaatac acttccgatg agtcaacgct
    27901 gctcatctcc acccaccagg cacgggaact agaggcgatc attgatccca taattatttt
    27961 ggaccgcaaa gatgtgttac tgaatgctac gctcagtgaa atcacgaaaa aaatctattt
    28021 tgattactca acggcaacct cccctgaagc tctctattcc gagctgatac ccggaggttg
    28081 cattcaggta ttggtcaaca agagcggcga ggagagtaaa gtcaacctgg aggccctttt
    28141 caatgcagtt cacaggcaca aagagcttat aaaaaagatg tttaacgatg aaatagcata
    28201 gttatgggaa gtaatgtttt aaatatgaat aggtcccggc gttattttcg atcggattta
    28261 cgtgctgccg tttcaaacta cggaataaca ttcttagttt tcagtacact tgctgtcact
    28321 acatatctgt tacacggcct ttttttcctg acaacttcgg gtgaatggta tgcaatgagt
    28381 tcttcacttc gtattgcact ttttgtcata tctggattaa cggtattaat cagttctcct
    28441 gccaaactct acggacatct tactgaccgt agggagggct cggcattcct tatggttccg
    28501 gtatcaaggt tagaaaagtt tgtctcaatg gtgctgatat cctgcataat cataccgttt
    28561 tcgttcgggt taatttatct gacgctagat tcccttgtat gtcttattgg tcctgcctgc
    28621 ggtgactctc tgttccaaat tatttttaaa agctctccat catttatcaa cacaatcccc
    28681 gatctgcaca tatataaaga aagtttccgc tcaattctga ctccctggct ttactttgat
    28741 gatattatcc agattgcgct cttgttcctt ctgggtgctc ttatatttaa aaagtctaaa
    28801 acaggcaaaa ctttaggctc ccttattatc atatccatct ctttgaatct tctgtttact
    28861 ccaatcatgt cgctcgccat gtttgacaag tttaggatga tggcagagat gatgagcgat
    28921 gccaacatca ctctcgaggc catagagcag atgtttccct ttttctcatg gattctgaga
    28981 aatctggcta tcgtggatac attgtccgat actctcgtga acggcctgct cctgttcttt
    29041 gtatggctcc ggcttaagaa acttaaacac taaccgctac agatatgtat tatggaattt
    29101 aatcagaaca aaccgatata tttacagata gccgacggaa tcctggaaaa gattctgagc
    29161 agcgagttaa aagagggcga caggatactc tccgtgcgcg aactgggtgc tgagttggga
    29221 gtcaacccca acacggtaat gcgcagttat gagaagctta ccgctgacgg cataatctac
    29281 aaccagcgtg gaataggata ttttgtggcc gacggtgcgc gcaacacatc tctggaaaaa
    29341 atgagggccg actttcttga aaatgaactt ccgcaaatcc tgcgcaggtt ggaacttttg
    29401 aaactggacc ctaaggatta tctgaaataa tccgattgaa ccgcagagcc gtgacagatc
    29461 tcataacggg gtggttcagc gaaaatcggg agacgtccgt cggtgtaaag agagcaccgg
    29521 aacgcgataa ataaagcccg ctgaaattct gaaactgttg tccggagtcg cgataaacgc
    29581 tacagtgggt ttgaggtaat tagcagaagt tgcgattaaa aacaggtgca aattatcgtc
    29641 tttatttgga taaaattcga taccggcctg gtaattccag gaggtcagac aattaccctt
    29701 gtccagaaaa atgcttgatt tataagttga aaaatcgtcg taaaaacatt tcaaaagaat
    29761 attccattgg ggctgaaacc ggtaatttgc ttctcccaag agggaaaaat agtttacatt
    29821 ttctaccaca ttttgtgggc tgatggtcaa gtttctaatt ccacccaaat aatcgatttc
    29881 ggaatgttga taaaaggcgt cgatgtaaat gctgaaagga cccgattccc atttttgtcc
    29941 accactcacc ataatcagcg ttttgccttt tgcgggcgaa gtgatattcg ccgcatatcg
    30001 aagttggaga gcgttatcca aatagttgct gttccagcca atcgagtagt ataggggcaa
    30061 ggtaggcgct tcaagttgta aacctatttg tggaataccg atggcttctc gccacgttcc
    30121 cattcgattg ttcactattt ggaaagaaaa ttcctggttt tgcgtgggag taaatgccac
    30181 ctgcaccccg gttaaataac aggaaataag atttgatatt ccgctgtagt cgtatacgtc
    30241 tacgggatat ttattgtatt caaagccgcc tatggagaga gcttgcttgc cggcagtcat
    30301 cgaaaaccgc tcgttaaacg cgtatatcag ataagcataa tcgatggtgg agggcaaatt
    30361 ttccttagtg aaaatgggtg aaaccttatt caagcgctgt aagtatctgt acgagagctt
    30421 tggtgtaagc tgccccccaa gatcgatcac tatatggtcg aatttaaatc caccgctttc
    30481 aaattccatc ggtgttccac caaattgagt atgataggct gggcgtaaat gcagtgagag
    30541 atcaaccgat ttagacgtat tttcatcgct cgattgtgcg aatagcgacg agctgcttaa
    30601 acaggcccaa aagacggtta aaataagtac gagggtaagg aggtcatttt ttttcatgcc
    30661 ggtttttaaa ttttaactta cgcaaataaa gtgattttat tgtcaggata aaacaccacc
    30721 acaaatcaaa cgatcagtga tataacccgc aaagttaaaa caaaagccga aataaaaagc
    30781 ggtat
//
LOCUS       DAOSTD010000013        30742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1641_length_30742_cov_14.617851, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000013 DAOSTD010000000
VERSION     DAOSTD010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30742
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1641_length_30742_cov_14.617851"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            487..1344
                     /locus_tag="PLI37_01580"
     CDS             487..1344
                     /locus_tag="PLI37_01580"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOQ57744.1"
                     /translation="MKNKNKISYIYVFLACLLLELIIVPIRGINLELSGLIAFVAYSL
                     FIYFCCKFFRKLNPLLILGFTFLAWFLLNLPVRLFYWKDALITLWEVLMHVLGMITGY
                     LLWVNKRIWKFVTLLVATIVCVVYAANNIKWLDYLSFGKVDSEGEQVLEEDFCLRDLN
                     GSELSLNDFKDKYLVLDFWSSSCGLCIKSFPEVQSFSEKFSNNQRVSFYSVFCVSAKR
                     CETRQTGFDIVQKRGFSFPLLFSEDESYKKFGVSCFPTVVIFSPDNKIIFRGRLEIAI
                     KFINKNINK"
     gene            complement(1569..1997)
                     /locus_tag="PLI37_01585"
     CDS             complement(1569..1997)
                     /locus_tag="PLI37_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57745.1"
                     /translation="MKRFSLVITLALLFVAGTITAQQQQRTGQRGQFSTTARAEREVE
                     QLKSSLSLSEQQAKDVFKICLKYAQQDSVRMAEMRSSGQEFDREAFMKQREEVTKAKT
                     AELNKVFTPQQQKDYAKQLEEAAQRRMQRQGAAPGQGGNR"
     gene            complement(2119..2856)
                     /locus_tag="PLI37_01590"
     CDS             complement(2119..2856)
                     /locus_tag="PLI37_01590"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOQ57746.1"
                     /translation="MGALKWIGGALGWALGGPLGGILGFIFGSIFESVSSGKYAYQQQ
                     DQIQGVRTGEGDFGLSLLVLSAAIMKSDQRVLKSELQYVKNFFRQQFGQQKAEQYILI
                     FRELIKKDYDLVPVCQQIRQAMDYASRLQLLHYLFGLGQSDGQLQEAEIRLIQSIASW
                     LGISAMDYESIRAMFIKDATSAYKILEISPEASVEEIKKAYKRMAVKYHPDKLEHLGE
                     DVQKKATEKLKEVNVAYEQIKKERGFN"
     gene            2953..3519
                     /locus_tag="PLI37_01595"
     CDS             2953..3519
                     /locus_tag="PLI37_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ57747.1"
                     /translation="MSGALNTDLLLIEACLKGDAKAQKELYEQYARKMMPICLRYSND
                     YETAKDLLHDGFVRVFTYLGDYKGEGSFEGWMRRIFVNTALEYLRKFKDKPHLMNIED
                     AYMLESNEHSVLEKMTEAEIIACIQELPTMYRSVFNMFVIEGYSHREIGELLKITESS
                     SRVYLTRAKQLMQSMLIKRYGKNERYRI"
     gene            3500..4822
                     /locus_tag="PLI37_01600"
     CDS             3500..4822
                     /locus_tag="PLI37_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOQ57748.1"
                     /translation="MNDTEYRRYFQQRLSNYESDEVPEFDQLFSKADLALVPAKSGGF
                     RLWPVVLSVASAAAVVALLLILLPAGRDSDYFSDREYRESFSLPDVSSLEIKSKIQAY
                     DEPVLLATSALPDNSDRHTIRRFETIGVQIPASPVSTGSEGVLSENAGDGQEAANAET
                     QQEQVSTGRSQVSEMIHQTTYERSVQEAHRLAAENARQRKRIFRPRLGVAINQNNGLL
                     ASVSPNSYQSMAASFANDYQNAYSVLNAASLMRSGEVDPNAWEKPGNLDMPVVEKPNY
                     HFPLNLSLSVSLPLSSRLELQTGLSYSYLSATTEGETNLYNFTLFQELHYLGVPLRIA
                     YRMIDSDRFGMYASLGGSVEKGLVGKQKNTVVTADNRRDVWTGQQSVHGIQPIASAQL
                     GFDYALNPKLQLYLEPGVVYYFDTDQPLSIRTKTPLFFNLGLGLRYRI"
     gene            4886..4956
                     /locus_tag="PLI37_01605"
     tRNA            4886..4956
                     /locus_tag="PLI37_01605"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4918..4920,aa:Gln,seq:ctg)
     gene            4967..5773
                     /locus_tag="PLI37_01610"
     CDS             4967..5773
                     /locus_tag="PLI37_01610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ57749.1"
                     /translation="MKPIRYLLFCLVLISWQLKAQKQDTIYISTSLGTMKAVFYDDVP
                     RHRSMYTERIKYEAFDGTLFFRVVPNFIIQGGSPDSRNAAPGERVGQGSNQFLLKPEF
                     RPAHFARKGAIAAPRQPDSMNPEKESDCSQFFIVKGRVYTGGYLDSLEMQRNNPLKNA
                     WWAVNYTPHKAEMDSLRKADPRQFNQRLRSYRAEMDSALRSNPQALFFSEEQRAILTT
                     LGGELSLDMQYTFFAEVVEGLEVIDKIAALKTDAYERPLQDVVIHSVRIK"
     gene            5784..6944
                     /locus_tag="PLI37_01615"
     CDS             5784..6944
                     /locus_tag="PLI37_01615"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HOQ57750.1"
                     /translation="MITTIVIVLFYLFVPAIVLRLCHLSKMADKIGAVLICYLIGMVF
                     GALVINDERYAIPDAISTIMIPLALPMLMFEVNIRDWSKMAGKAFLSMLAGVFGLVIV
                     VVLGYFLFKESVPEAAKVSGMLAGVYTGGTPNLASIKAALGVPEDLYIAVNTVDMLVS
                     AAYLLFLLSYAKPLLSKILPPYQSKHGVQQIETNSLLQEEPKLALIPWLKKVKTRHIL
                     AAFGVTLLCAAGGAGLGLLVKNPAYQMAVIILSITTLGIIAANLKPVKKIQGSFDTGM
                     YLILIFSVAVASMADFKDISSKLLPLIGLLSFVIFGSLLLHMLTCKLFRIDTDTAMVT
                     SVALVCSPPFVPVVCGAIKNREVMISGITVGVVGYALGNYLGIAIAYFLQGI"
     gene            complement(6950..8908)
                     /gene="gyrB"
                     /locus_tag="PLI37_01620"
     CDS             complement(6950..8908)
                     /gene="gyrB"
                     /locus_tag="PLI37_01620"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOQ57751.1"
                     /translation="MAEEEIVNNEQSYSAKNIQVLEGLEAVRKRPAMYIGDTDEKGLH
                     HLVYEVVDNCIDEALAGYCSDIRVWINEDGSITVSDNGRGIPVDMHEKEGRSALEVVL
                     TVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSDRLFVQIFRNGKVYEQEYHKGKPLYP
                     VKETGTTDRTGTTITFKPDSEIFTTLEYKYDILANRMRELAYLNAGISLTMTDKRVKN
                     EDGSYKNELFYSEQGLSEFVRFIDTSREPLIEEVIHITTDKYGTPVEIAMIYNNSYAE
                     NVHSYVNNINTIEGGTHLTGFRRGLTRTLKKYAEDSKMLEKLKIEISGDDFREGLTAV
                     ISVKVAEPQFEGQTKTKLGNTDVIGIVDQTVSEALGYYLEEHPKEARIIVDKVILAAT
                     ARHAARKARELVQRKTPLSGGGLPGKLADCSSRDPQKCEIFLVEGDSAGGTAKQGRNR
                     EFQAILPLRGKILNVEKAMPHKVLESEEIRNIYTALGVSIGTEDDSKELNISKLRYHK
                     IIIMTDADVDGSHIDTLIMTFFFRYMKPLIEQGFLYIANPPLYLCKKGKIEEYCWNDK
                     QRQRFIEQHAGGNESAVHTQRYKGLGEMNAEQLASTTMDPEHRILRQVTIENAAAADH
                     IFSMLMGEDVGPRRNFIEANATYANIDA"
     gene            9085..9156
                     /locus_tag="PLI37_01625"
     tRNA            9085..9156
                     /locus_tag="PLI37_01625"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9117..9119,aa:Met,seq:cat)
     gene            9248..10615
                     /gene="radA"
                     /locus_tag="PLI37_01630"
     CDS             9248..10615
                     /gene="radA"
                     /locus_tag="PLI37_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOQ57752.1"
                     /translation="MAKAKTIFLCSNCGSDFPKWMGKCPVCGEWGSINEEIIHRDATS
                     KQLTSQFNAEKSQPQLLAEVEFNHEARISTGGPELDRVLGGGLVQGSVVLLGGEPGIG
                     KSTLVLQTAIKMTHKRVLYVSGEESLQQIKMRAERIKGDNPQAYFLAETSLENIFEQI
                     SRLKPELLIIDSIQTLSTEVADSSPGSVTQVRECATALLKVAKTLGMPIVLIGHINKE
                     GSLAGPKVLEHIVDTVLLFEGDTHHLYRILRSVKNRFGSIAEIGMYEMRSDGLREVTN
                     PSELLLSQHQDGLSGVAIAAAIEGIRPFLIETQALVSTAAYGTPQRSTTGFDLRRLNM
                     LLAVLEKRAGFKIAQKDVFLNIAGGIRVNDPAIDLSVIAAILSSNLNLSIGNKVCLAG
                     EVGLAGEIRPINRLEQRIMEAQKLGFEQIIVPDFSESATDLRRFKIRIDKVRKVEESF
                     RLLFG"
     gene            10713..11288
                     /locus_tag="PLI37_01635"
     CDS             10713..11288
                     /locus_tag="PLI37_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOQ57753.1"
                     /translation="MKEQRMIGSRIKGMRESKSISIEVFSERSGLSVEQIQLIESNSQ
                     IPSLAPLVKMARALGVRLGTFLDDNEDLGPVVSRKEDQPAGISFSSNTASSHNDLQFF
                     SLAQKKAGRSMEPFIIDIQSSTQNGYALSSHEGEEFIYVLEGEIEIFYGKRVFKLSQG
                     DSIYYDSIVDHNVHAANNSEARILAVVYTPF"
     gene            11290..12933
                     /locus_tag="PLI37_01640"
     CDS             11290..12933
                     /locus_tag="PLI37_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ57754.1"
                     /translation="MELSNRTVGEFLEHWATTTPDREYLIYSDRDLRFTYKQFNQRVD
                     NMAKGLMAIGVRRGSHIGIWATNVPDWLTFLYASAKLGAVLVTVNTNYKQHELEYLVE
                     DSDIETLCITEGVFDGNYVDMVNTMLPELKTSQRGHLQSNRFPKLKNIVYIGQEKYRG
                     MFNTAELLLLGQNIDDDELNEIKRSVNCHDVVNMQYTSGTTGFPKGVMLTHHNIVNNG
                     YFTGEHMAFTSEDKLCVPVPLFHCFGVVLATMNVLTHGCTQVMVERFDPLVVLASVQK
                     ERCTALYGVPTMFIAILDHPMFEMFDVTSLRTGIMAGSLCPVELMRRVTDKLHITHIT
                     SVYGLTESSPGMTQTTVNDSFEIRCTTVGRSYPFTEVKVVDPETGKECPPGVQGEICC
                     RGYLLMKGYYKNPQATAEVIDKDGFLHSGDLGIVDENGYYRITGRIKDMIIRGGENIY
                     PREIEEFLYHLEGIKDVQVAGIPSEKYGEEVGAFIILHEGVSMDEEYVREFCRGKIAR
                     HKIPKYVFFVDSFPMTGSGKIQKFKLKDVGLKLLEKRGD"
     gene            complement(12930..13838)
                     /locus_tag="PLI37_01645"
     CDS             complement(12930..13838)
                     /locus_tag="PLI37_01645"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOQ57755.1"
                     /translation="MNHIKNILFLLLLPGLLGACQQKDNYLVADNEILMMEDVSFESR
                     EDLKFKGRVAGAQTYAAGNVAASESTITDEAVKQGSEATDKKKIIRDGHMTIKVKSAQ
                     AAKTEVDSLLLPFGAYYASENFNNNEREATFYLRLRIPAAAFDDFILCLEKGGYGEIL
                     NKDIQARDVTDQFIDLETRLQNKRNYLSRYKDLLKEAKTVKDILQIQEEIRGLEEEIE
                     STTGRLKYLSDQVDYSSLQLTLTEQKDFQYKPEQRDRFGEKLKQALTKGWYGVVDTVI
                     FLFKIWPVWLILGLALVFLRRRNKPA"
     gene            complement(13835..14842)
                     /locus_tag="PLI37_01650"
     CDS             complement(13835..14842)
                     /locus_tag="PLI37_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ57756.1"
                     /translation="MEYRKIGKTDLELSVITFGAWAAGGMMWGGTKQNDAVNAIRASY
                     DMGVTSIDTAPVYGMGYSEQLVGEAIKGLPRDKVQILTKYGLRWDTTEGEYYFDSVSN
                     EGKTVHIYRNARKENIIQECETSLKLLGTDYIDLYQIHWYDPTTPVGESMEAVSRLME
                     QGKVRYAGVCNYSADQMEEAAAFIDLASDQLPYSMLRRGIEDETVPYCIKHNKGILAY
                     SPLQRGLLTGKMKPGQQFDPNDSRTGQVWYLDENIVQVNNFLNRIRPIAEAHKASLSQ
                     VVLRWTVEQPGITVALAGARNVAQATENARAGMLKLSPDELRIIDTELSKINLVFPEK
                     K"
     gene            15057..16730
                     /locus_tag="PLI37_01655"
     CDS             15057..16730
                     /locus_tag="PLI37_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOQ57757.1"
                     /translation="MKLRSHFTGLILFAFFSIALQAAVPAFPGAEGAGAYTTGGRGGK
                     IYRVSNLNDSGPGSLREAVQARGKRIVIFDVAGIIDLQSTLKITNGDITIAGQTAPGK
                     GICLRNYPLQVAADNVIIRFIRSRMGEEAGVEGDAIWGRYRQDIIIDHCSFSWSVDEC
                     ASFYGNKNMTMQWCTICESLNLSVHVKERHGYGGIWGGSPASFHHNLIVHHTSRTPRL
                     SGGDPATEMVDVRNNVFYNWGPTNGGYGGTGGSYNFVGNYYKPGPSTAVKDFLCNRIM
                     YTGPSENPDSAGNYIWGIFHFDDNYFDDSCPDLSETAINNCSRVNSNNYVGLHLSTST
                     NLPGGTKYGIQSSTPFSCLPVSTHQPQDAYDKVLELAGASLKRDKQDQRYVAELIDGI
                     YTYEGSRGSTNGIIDSQNDVGGWDTYTGRNTEGDSDGDGMPDNWEDIHSLDPADPSDG
                     PAYTLSQQYTNVEMYLNSLVASIMEAGLEGAESNLRSVDKPDDTPSYLWTEAGLLVPG
                     AVSLELYTMGGLLLAKAQGSLLEISPTPGFFLLRVKDAAFREHSISLVK"
     gene            16751..17215
                     /gene="bcp"
                     /locus_tag="PLI37_01660"
     CDS             16751..17215
                     /gene="bcp"
                     /locus_tag="PLI37_01660"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOQ57758.1"
                     /translation="MRMLQKGEKAPDFKGVNQQGEIISLSDFRGKRVILYFYPKDNTP
                     GCTAEACSLNDSRSYFLDKGFVIIGVSPDSVESHQRFAAKYGLSFNLISDPEKAIIQA
                     YGAWGEKKNYGKTYMGLLRTTFVISADGIIEEVFPKVDTKKHAEQIIKALGI"
     gene            complement(17219..19948)
                     /locus_tag="PLI37_01665"
     CDS             complement(17219..19948)
                     /locus_tag="PLI37_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOQ57759.1"
                     /translation="MKKHFTNSIWLPALLAVFLSFGINSCKDRPKEKANLAVLAKVQG
                     QRGGAETHPLNDGEIPNELSEQQRNRMMQAVEQANQPQYRRYNQLVSQQLEYRWASPV
                     TIDEVQLYWFNYEGLAKLPQAHRLSYWDGQQFVPVSNAQGYGLENNQFNVTVFDEVTT
                     TALRLELDSLPRYPSTLLEWRVMKSLNSPAVAPMVTAGIDRDVMVGGNTYLNAEIKAV
                     DPVKKLRWSAKGPGKVSFADPKALTTTASVDKPGKYILTLSAQSGKMKSGSSLELIAH
                     YPPQEERLDVVYTKRYKINSPLWNERAKVLITNWIPWCIDQCERRDLTQGQGGLDNFV
                     EAAKALRGEPHGRHLGYVFSNAWVHQTVESMCIALMVDPQGDKEIIAAQNKMKKALER
                     WIPIILSAQEPDGYLHTAYTLRDTTRWTGRWSPRNRSDHEGYVAGYFIESAINHYTLT
                     EGKDTRLYDAAKKLADCWVKNIGPGPDQIEWYDGHQEMEQALVRFGRFVNDMEGNNKG
                     DSYIALAKFLLDMRDKGGEYDQSHLPVQQQYEAVGHAVRAVYTYSGMSDVAAETGDID
                     YQSAVMSLWNNMVNRKYYVTGGVGSGETSEGFGPNYSLRNNAYCESCSSCGLIFFQHK
                     MNIAYHDAKYADLYEETMYNALLGSIDFEGKRFYYTNPLTAYNARDPWHVCPCCVGNI
                     PRTLLMIPTWTYVTGKDGIYVNMYVGSTINVERVAGTDVEMVQQTDYPWKGAVSIIVN
                     PKEDKHFTVYVRVPNRKTSELYTSVPELNTLKSLSVNGEAVEMKADRGYIAIARDWKK
                     GDRIDLEIPLEVQQITADEKVEALSGLVALRYGPMIYNVERADNKDINQAVGSEPFKA
                     VWRSDMFKGIVSIEGKWADGSDLLAIPNYLRMNRIQPVQEGRRSPESMVWMRK"
     gene            complement(20188..23001)
                     /locus_tag="PLI37_01670"
     CDS             complement(20188..23001)
                     /locus_tag="PLI37_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HOQ57760.1"
                     /translation="MSDAQGTFQHRATGSTTQTDRRFEGTAVHTRAEAESLPATNPIH
                     PQPVADRIVPFNLSDSGKHTPILWGLDTAWPSRDNIIRGVAFMGSENIYLVRASFQPT
                     MPLVNGDLQSQQVADLNNRLNLINLTGSHTKVALNCDHPSVDPWYAGNAQNWAQLMDV
                     TARRIQERGRQIVSIAPFNEPDYGWGQGSLTDFFNIAGELRKNPRFDSIRISGGNTLN
                     TDQALSWYNSLKSRLDEGNTHQLAGSFDNYASFFRTVRANGHHATNDELHNVMEAMVG
                     VEYGMQTGIWWGTAELARGEFVKASFGRRLAYAEHRPNWTAATVYRSPEGKVQAFVGS
                     SERQAATTSYSFISSERDVFYDGHGPQRAFTIEIPGGTGYQQGQTNAERVININWGED
                     VPQPVNGRYVLVNRNSAKVMEVENGSTSAGSNVRQNTYTGAAYQQWVVTPVDPRIGGD
                     FSYHSIIANHSGKSLDVYNWSLNNGGNIIVWDDAKGANQQWYLEYAGDGWFYIRSRHS
                     SHCLQIQNASTANAANIQQGTKNGSANQQWRFLPVGAAIEFNPPAAPAEISAAANSHS
                     LRLDWAAVGDADLAGYTIYRSDSLQGSFQTLARNINGTSFVDHSIEAGKTYYYAVKAV
                     DRSLNHSAFSDTVPAVASGGRDMLAWLSFEDNTLDNTPHLNHGEGLEGLSFVEGINAS
                     RALTLDGTRGFVQLPSTLGNHPELSLALWVNWQGGDSWQRIFDFLYDQTSYFYLTPKI
                     GVFNKMRFAIRNKGGEERLEAAALPKNQWCHVAVTLSDSLACLYVNGEIADQSTEFSL
                     NPADVRPIINYLGRGQHVSVLFKGQLDDFRVYSYPLSTDEVAALAANGPDALPEISGS
                     EINELKIWPMPARDLLYLDCGRDHRRTGQIDLYSLDGNKVATYTLKGSKAELDLSHLA
                     AGIYILQLKHGGSRTFRTIVVEP"
     gene            23197..>24704
                     /locus_tag="PLI37_01675"
     CDS             23197..>24704
                     /locus_tag="PLI37_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="silent information regulator protein Sir2"
                     /protein_id="HOQ57761.1"
                     /translation="MKTKSTLVRFALGGGATLLFLFAGLFKTGAQDPRERYYYYEILE
                     PHHAPKPQVEGFATMRVNEALDRGLTATRAQDGQSLYLSWRLLAEDPQDVAFDVIRVE
                     GQKNQRLNKRPITATCDFIVENPPAEAVYKVVPRNNRKSVNSAGAKAAANPGTAAASL
                     DKTKGVLNTGEGSIGIRMEDLPAFPYLSIKLRDGVRPGKMALADLNGDGIYDYIIRTP
                     DSSVDPGMPGDLAGRTYQIEAYLSDGTFLWSFDLGQGIEPGVWYSPFICYDFNGDGKA
                     EVALKTAPGDIVRNEKGRVDYGDEYLSVLDGMTGREIDRVDWPVRNFRYGDLNRQSRH
                     QIGMAFLDGKTPYILAARGTYKLMTVDAWMLRGDKLELAWRWDGDEENPVVRSMGAHN
                     MICTDVDGDGRDEVVLGSCALDDNGTLLWSTGLGHPDKIFISDIDPNRPGMEMLLCLE
                     PWHDNGRGVCLIDPKDGSPIWNIGHKTYHVGDGMVADFDPSSPGLECFASED"
     assembly_gap    24705..24804
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24805..25289
                     /locus_tag="PLI37_01680"
     CDS             <24805..25289
                     /locus_tag="PLI37_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="silent information regulator protein Sir2"
                     /protein_id="HOQ57762.1"
                     /translation="ADFDPSSPGLECFASEDQKGGSSDKYLLSADGRIIGKNEEVPPC
                     RNWAWWDADLLREYISAQFYRSPEGRFGRKSGIGKWKGETLFGEMEGSILMIADVFGD
                     WREEIITALPGELRVYTTNIPARDRRITLMQDALYRNYVAHRSMGYDQAPVPSFYLGE
                     "
     gene            25395..27977
                     /locus_tag="PLI37_01685"
     CDS             25395..27977
                     /locus_tag="PLI37_01685"
                     /inference="COORDINATES: protein motif:HMM:NF025938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOQ57763.1"
                     /translation="MRSFSLNLKIVLLLIFAGQSIGGIAQVIEPESDKDYYLIHSGAC
                     VLSRADDGRAVVQTAGATGTQVLNFVADGRGYYTVKLKDRREYMALSGSWNTYFVSDS
                     ASDAAKYAVEEVRKGVVRLRCKANRKYLGTDNSTAGSHVYSDKSGSDERHYWLISEEI
                     REFPVDTDSLYYLINPSDAFGKTFEGWGVSLCWWANMCGRWEENKIDEIIDWLVSPEG
                     LNYRIFRYNIGGGDDPLNRNCDPHHMALGKGLRAEMEGFKDSADAPYDWSRDAAQRKI
                     MLKIREKRPDAVFEAFSNSPPWYMTYSGCVAGNEPAGHDNLKPEYYEAFATYLVDVCR
                     FYKDSFDLEFKTLEPFNEPVTDYWGRNGGQEGCHFSTASQIAFLRVLAPKLEASGLNT
                     VISASDESYTDQSVRDFLAYRNGQVIALTGQWNTHTYSANNHSRAALRELASAEDITL
                     WMSEVGAGGSGLAGNLSLAQKLIDDIRYIRPEAWIDWQYVEENNDQWCTVRGSFQNQR
                     YWKVKNFYVRRQFTQFIQAGWRFLNVPNNKMLSALSPGGDSLIVVALNTSTVRPAYHS
                     IDLSLFDEIYRGITMSRTSQSENNQTVNDFVWRDSCLQFVLPPQSIGTLMIPVAFTPE
                     TNTEPVSGRPYLIASRCSGLMLQAEGDGVLINDYRPADSSQLWLLQKTGEGYTIGNLQ
                     GLRLNDDGSYFLKTTSTFDPNNQLFDLDLRNDNFFKISSLSTGKALDLQGANYTPGTR
                     VGLYGYDEGGADKVVTRQWMFLRPPRRLSDFNTSLFPPEHETALEVQLSVAPGMVQLF
                     CPSFGHDGKTYRLRLFNLSGIMVRESRLNSAITRLSLPEGFYLLSIDAGAEEKIFVQK
                     IIVP"
     assembly_gap    28035..28134
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            28146..>30742
                     /locus_tag="PLI37_01690"
     CDS             28146..>30742
                     /locus_tag="PLI37_01690"
                     /inference="COORDINATES: protein motif:HMM:NF027309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HOQ57764.1"
                     /translation="MRTPNLYCIKTGLPLILLCFAVFCGSCRQGSTGGSRTELLFERE
                     KTTTLWLDTGEAEVVYTAVDLFRRDVQLVFNADLKIVEDKKDADIVLAFSGLKRPASS
                     GPKKFSPSWLKRSAVAGPKKLAFFKCHRSSFSKPNSRGGEAFRIRLCKSGRKPQLIVS
                     GSEPRGLAYGILELSRMIGVSPWHYFADSRPKELESFEFPLKRIEQQASVKYRGIFIN
                     DEDWGLMPWASQTLAPQQGKGVIGPEAYEKIFELLLRLRANTIWPAMHECTRPFYQVE
                     GNAQMARKYGILLGSSHCEPLARNSASEWDMDGSGDYNFMTNPSNVIQYWAGRLQELS
                     PGENIFTLGMRGKHDGLMQGVKTLEEHKAALSRIIPVQQDLLKQYIDADIEAIPQVFI
                     PYKEVMEVYDAGLEVPDHVTLVWCDDNYGYIRRLSNAREQERSGASGIYYHVSYWGRP
                     HDYLWLASTSPGLIYSEMLRAYKHGADRLWILNVGDIKPAEYLMEYFLDLAWDINAVD
                     NDFGHLQRFMHREFGAEQATALSEVYERYYRLATIRKPEFMGWSRVEESGYGRGGKTP
                     VRDTEYHPEFNKELQHRLEAYREQEQRVAEIRLRIPEQQLSCFFQLVEYPIRAASLMN
                     QKWLYARLARYYSDSRPELARLCAALSLQAYQGIEQLTATYNSLEQGKWERIMDFRPR
                     ELPVFDKPVFNNPELDGPEQKSSEFGKFLFEGPEFEKLFDHTLENNRRLYDSLISQTV
                     QSADSASLVDNSRPFVTACNAARARSVRGKVRSIRGLGHSFEAVQMAQGSSVNYRFDL
                     PESGEYRIVIAAVPNHDVDGQGMKIRVAADGRDLGEFDYKTRGRSEAWKQQVLRGQAL
                     IEIPAREME"
BASE COUNT         7522 a         6995 c         7692 g         8333 t
ORIGIN      
        1 cttcccgggc gcagcccgaa aagtagcgtt tagcatagag ggcgcagccc gaaaagtagc
       61 atttagcgta gtagggcgca gcccgaattt aaatcaatat cactaaattc cagattaaat
      121 cgaagagttt aaaatgatag aaatgtgtta acataccccc ctataagagt tattaaagac
      181 aacgctcttt gttgttgttt aataaaatag tattactttt gcgataattt tttaggaagc
      241 ctcaccatct ttggtctata aaaaccataa aagacacata ttatattatg cccaaacgat
      301 actgtatttt acttactctg gcaaccatcc ttttcacaca tgtatcggtt ggttcctttg
      361 ccgataggaa gaattcggtt tttcttgagg gctctctgtt gtacaatttg ggattgttcg
      421 gaattacaaa aatgaagata aattcaataa tctttttctc catattacaa atattataac
      481 atcatcatga aaaataaaaa caaaatatca tacatctatg ttttccttgc ctgcttactg
      541 ttggaactga ttattgtccc cattcgtggt ataaatttag agctttccgg attgattgct
      601 ttcgtggcat atagcttatt catttatttt tgttgtaaat ttttcaggaa gctcaatcct
      661 ctccttattt taggctttac atttcttgcc tggtttctgt tgaatcttcc tgttcgattg
      721 ttttactgga aagatgcgct gatcactttg tgggaagtgt taatgcatgt tttagggatg
      781 attacaggct atttactatg ggttaataaa agaatctgga aatttgttac actcttagtt
      841 gcaacaatag tatgtgttgt gtatgccgcg aataatatca aatggcttga ttatctgtcg
      901 ttcggcaagg tagatagtga aggcgaacaa gtgttggagg aggatttctg tttacgtgat
      961 ttgaatggaa gtgaattatc gcttaatgat tttaaagata aataccttgt gttggacttt
     1021 tggtcttcat cctgtggttt gtgtattaaa agctttcccg aagttcagtc gttttctgaa
     1081 aagtttagca ataatcagcg agtttcattt tattctgttt tctgtgtatc cgcaaaaaga
     1141 tgcgaaaccc ggcaaaccgg attcgatatt gttcaaaaaa gaggattcag ttttcccttg
     1201 ttgttttcgg aagacgaaag ctataaaaaa tttggcgtca gttgctttcc tactgtcgtg
     1261 attttttcgc cagacaataa aattattttt cgcggcagac ttgagattgc aattaaattt
     1321 ataaacaaaa acataaataa ataaaatatg tatgtatcat aatgtgatga acactataca
     1381 tagaagcttg acaggaaaag gactgattgg gtcgttgctt ttttataagg ggcggaaaag
     1441 caaaaaaatc cgaccaaggc cagcattgat taaattttag aacaaagaag ctgtctcccc
     1501 gcttacgcaa aagagacagc ttctcaaccc aaaaattaaa ccttcatcaa actcaaaaat
     1561 cctccacctt atctgttgcc accttgtccg ggagctgcac cctgacgttg catgcgtctt
     1621 tgggctgcct cttccagttg tttggcgtag tctttttgct gctgaggtgt aaacaccttg
     1681 ttcaattcag ctgtcttggc cttggttact tcttctcttt gtttcataaa ggcctcgcgg
     1741 tcaaattctt gaccactgct tctcatctcg gccatacgga cggaatcttg ttgggcatat
     1801 ttaagacaga tcttaaacac gtcttttgct tgctgttcac tgagtgaaag gctgcttttt
     1861 aattgctcta cttctctttc ggcacgggcg gtggtgctaa attgaccgcg ttgaccggtt
     1921 ctttgctgtt gctgagctgt aattgttccg gccacgaaca gcaaagccaa cgtaattact
     1981 aatgaaaacc ttttcataaa aatgaggatt aaaaataaat aaagtgaatt cgctaaactg
     2041 ggtattagag accatttggt cggaatcgtt taatgcccgg taagcaaata atgataaaat
     2101 aacatcaata aacacttctt aattaaatcc cctttctttt ttgatttgct cgtaggctac
     2161 attcacttct ttgagttttt cggtagcctt tttttgtacg tcttcgccta aatgttccag
     2221 cttgtcggga tggtatttga cagccattct tttataggct ttcttaattt cctccacact
     2281 ggcttcgggg ctgatttcaa gtattttata ggcgctggtg gcgtctttaa taaacatagc
     2341 acgtatcgat tcataatcca ttgccgaaat gcccagccag gaggcaatgc tttgaatcaa
     2401 ccggatttct gcttcttgta actggccgtc gctttgaccc aatccaaata aataatgtag
     2461 gagttggagg cgtgaggcgt aatccatggc ttgtctgatt tgttgacaaa ccggtaccag
     2521 atcgtagtct ttcttgatca gttcgcgaaa tatcaaaata tattgttcgg ctttttgttg
     2581 tccgaattgt tggcggaaga aatttttcac atactgaagt tccgatttta atactcgttg
     2641 atcggatttc atgatagcag ccgaaaggac cagtaaactc agtccaaaat caccttctcc
     2701 ggtgcggact ccctgaattt gatcttgttg ttggtaagca tatttaccgg aactgacact
     2761 ttcaaatatc gagccgaaaa taaaaccgag tattcccccc aggggaccac ccagagccca
     2821 acctaaggct cctcctatcc attttaatgc acccatttgt aaaggaaaat aagtataaaa
     2881 tcaaacggca aagatacata aattcattat tccttatctt tgtaacattt tcgaacaaaa
     2941 agcatctata ctatgtccgg tgccttgaat accgatctgt tgttgattga agcatgccta
     3001 aaaggagatg ccaaagcaca gaaggagttg tatgagcaat acgcacgaaa aatgatgccc
     3061 atatgtctcc ggtattcaaa cgattatgag acagccaaag acctattgca cgacggattt
     3121 gtgcgggtat ttacttattt gggcgattat aagggcgagg gctcattcga gggctggatg
     3181 cgaaggatct ttgtcaatac tgcattggaa tatctgagaa aattcaagga taaaccgcat
     3241 ttgatgaaca ttgaggatgc ttatatgttg gagagcaatg aacactctgt gttggaaaag
     3301 atgacagagg ctgaaataat cgcttgcatt caggaattgc cgaccatgta ccgcagcgtg
     3361 ttcaacatgt ttgttataga aggttattcc cacagagaaa ttggagaatt actaaagatt
     3421 acggaaagtt cctccagagt ttacctgacc agagcaaaac aactaatgca gtcgatgttg
     3481 attaagagat atggaaaaaa tgaacgatac agaatatagg cgctattttc agcaacgttt
     3541 gtcgaattac gaaagcgacg aggtgccgga atttgatcag cttttttcca aggctgattt
     3601 agctcttgta cctgcaaaat cgggcggttt ccggttatgg cctgttgtac tcagcgttgc
     3661 atcggccgcc gccgtcgtcg ctttattgct gatactattg ccggcgggta gagactcgga
     3721 ttatttcagc gacagagaat accgggagtc tttttcactt cccgatgttt cctctttaga
     3781 gatcaaaagt aaaattcagg cttacgatga gccggtacta ttggcgactt ctgctctgcc
     3841 ggataattca gacaggcaca caatacgacg tttcgaaacc ataggtgttc agattcccgc
     3901 atcgcccgta agtaccggct ccgagggtgt tttatccgaa aatgccggtg atggacagga
     3961 agcagctaat gcagaaacac agcaggaaca ggtatcgacc gggcgtagtc aggtaagtga
     4021 aatgatccat caaaccactt acgaacgaag cgttcaggag gcgcacaggt tggcggccga
     4081 aaatgcccgg cagcgtaaga gaatcttcag gcctcgtttg ggtgttgcca ttaatcaaaa
     4141 taacggtttg ttggcttcgg tcagccccaa tagttatcaa tcgatggcag cctcgtttgc
     4201 caacgattat cagaacgcct attctgtgtt gaatgccgcc agcctcatgc gcagcggaga
     4261 agttgatccc aatgcttggg aaaaaccggg taatctggac atgcctgtgg tggagaaacc
     4321 caactatcat ttcccgctca atttatccct cagtgtttcg ctgcccttgt cctccaggct
     4381 ggaattgcag acaggactaa gttattccta cttatcggct acgacggagg gagagacaaa
     4441 tctgtataat tttaccctgt ttcaggaact tcattatttg ggagtgcctt tgagaattgc
     4501 ctaccggatg atcgacagcg atcgttttgg gatgtatgcc tcgctgggag gtagcgtaga
     4561 gaaaggtttg gtcggcaagc aaaaaaatac tgttgttacg gccgataaca gaagagatgt
     4621 atggacgggg caacaatccg tacatggcat acaacccata gcgagtgctc agttgggctt
     4681 tgattatgct ttgaatccca aattgcagtt atatcttgaa ccgggagttg tttattattt
     4741 cgatacagac cagcctctga gtatacgtac taaaactccc cttttcttca atttaggcct
     4801 gggtctgcgt taccgtatct gatttttccc ctttttctgt tttgagcgta aaaaatattg
     4861 tgtactttta cgccgttttt tataatgctc cgttgtgtaa tggtagcact acagattctg
     4921 gatctgtctg tctgggttcg aatcctagcg gagcaacaaa ttcaccatga agcccattcg
     4981 atatctgctc ttttgtctgg ttttgattag ctggcaactc aaggctcaaa aacaggatac
     5041 aatttacatc tctaccagcc ttggtacgat gaaggctgtt ttttatgatg acgtaccccg
     5101 gcataggtct atgtacacag aacgtattaa atacgaggct tttgacggca cacttttttt
     5161 tagagtggtg ccgaatttta ttatacaggg gggatctccc gattcccgca atgcagctcc
     5221 cggagaacgt gtaggccagg gcagtaatca gtttttgctt aaacccgaat ttcgtccagc
     5281 ccattttgcc cgtaaaggtg ccattgcagc tccaagacag ccggacagta tgaatcccga
     5341 aaaggaatcc gattgcagcc aattttttat agttaaaggc agggtgtata ccggaggtta
     5401 cctggatagc ctggaaatgc agcgtaacaa tcctctaaaa aatgcctggt gggcagttaa
     5461 ttacacgccc cataaggcag aaatggacag tctcaggaaa gccgaccccc ggcaattcaa
     5521 tcaacgtctt agatcctatc gtgccgaaat ggattctgct ttgcgcagca atccccaggc
     5581 acttttcttt tcggaagaac agcgcgcaat cctcactacg cttggaggag aactcagcct
     5641 ggatatgcag tatacttttt ttgccgaagt tgtcgaaggt ctggaagtca tagataaaat
     5701 agcagcactc aaaacagatg cctatgaacg cccccttcaa gacgtagtaa ttcattctgt
     5761 tcgcattaaa taaagccggt tttatgatta caactattgt aatagtcctg ttttatcttt
     5821 ttgtaccggc catagtgttg cgtttgtgtc atctgtccaa gatggccgat aaaataggag
     5881 cagtgctgat ttgctatttg ataggtatgg tttttggggc tctggtcatt aacgatgagc
     5941 gttatgccat tcccgatgcc atttccacca taatgatacc tttggctctg cccatgttga
     6001 tgttcgaagt taatattcgc gattggagca aaatggccgg taaggccttt ctttcaatgt
     6061 tggccggagt cttcggtctg gttattgtgg ttgtactcgg atatttcctg tttaaggaat
     6121 ctgtaccgga agcggcaaaa gtgagcggaa tgctggccgg agtctatacc ggaggtactc
     6181 ccaatctggc ttctattaaa gccgctctgg gagtacccga agatttatat attgccgtta
     6241 atacggtgga tatgcttgtc agtgcagcat atttgctctt tttgctttct tatgccaagc
     6301 ctttgctgag caaaatactt cctccgtatc aatcaaaaca cggagtacaa caaatcgaaa
     6361 ccaactcttt actgcaggaa gagccaaaat tggccttgat cccctggctc aaaaaagtaa
     6421 aaacccgtca tattttagct gcttttggag tgactttgct ctgtgcggcc ggtggtgccg
     6481 gtttgggatt attggtcaag aatccggctt accagatggc cgttattata ttatccatca
     6541 ccaccctggg tattattgcg gctaatttaa aaccggtgaa gaagattcag ggaagttttg
     6601 ataccggcat gtacctgatt ttgattttca gtgttgctgt agccagtatg gccgatttta
     6661 aggacatttc atcgaaactt ttgcctctga tcggtttact gagtttcgta atttttggtt
     6721 ctttgttatt gcatatgttg acgtgcaagt tgtttcgtat tgataccgac acagctatgg
     6781 tgacatcggt ggcattggtg tgctcacctc cctttgtgcc ggtggtatgc ggagcaataa
     6841 aaaaccgtga agtaatgatt tccggaatca ccgtgggcgt agtcggctat gccctaggga
     6901 attacctggg catagccatc gcctattttt tacaaggtat ctgattgttt taagcgtcga
     6961 tgttggcgta ggtggcgttg gcttcgataa agttgcgacg gggtcctacg tcttcgccca
     7021 tcagcatcga aaagatgtga tcggcggctg cggcattttc tatagtaact tgtcttaaaa
     7081 tacgatgctc cggatccatc gtggtgctgg ccaactgttc cgcattcatt tcacccaaac
     7141 ctttgtaacg ctgcgtgtga acggcgcttt cgttgcctcc ggcgtgttgt tcgataaaac
     7201 gttggcgttg cttatcgttc cagcagtatt cttctatttt gccttttttg cagagataga
     7261 gcgggggatt tgcaatgtac aggaagcctt gctcgatgag cggcttcatg tagcggaaga
     7321 agaaagtcat tatcagagtg tcgatgtgac tgccgtccac atcggcgtcg gtcatgatga
     7381 tgatcttgtg gtagcgtaat ttggagatgt tcaattcttt gctgtcgtcc tcggtgccga
     7441 tacttacgcc taaagcagta taaatattgc ggatttcctc gctctcgagc accttgtggg
     7501 gcatggcttt ttccacattg aggattttac cgcgcaaagg caggatcgcc tgaaattcac
     7561 gatttcttcc ctgtttggct gtgccgccgg ctgaatcacc ttcaactaag aatatctcgc
     7621 atttttgggg atcgcgactg gaacagtcgg ccagtttgcc tggaaggccg cctccggaga
     7681 ggggtgtttt gcgctgaacc agttctctgg ctttacgggc ggcgtggcgt gcagtagcag
     7741 ccagaatcac cttatccaca ataattctgg cttcttttgg atgttcttcg aggtaatatc
     7801 caagagcctc actgaccgtt tgatcaacta tcccgattac atcggtgttc cctaattttg
     7861 ttttggtctg gccttcgaat tgaggttcgg ccaccttgac cgaaattacc gctgtcagcc
     7921 cttctctaaa gtcgtcaccg gaaatttcaa tttttagctt ttcgagcatt ttggaatctt
     7981 cggcgtattt tttcagagtg cgtgtaaggc cacggcgaaa accggtcaga tgtgtaccgc
     8041 cttcgatggt attgatattg tttacgtagg aatgaacgtt ttccgcatag gaattgttgt
     8101 agatcatagc aatttccacg ggagtgccgt atttgtcggt tgtaatatga atcacttcct
     8161 cgataagcgg ttctctcgat gtgtctataa agcggacaaa ctcgctcaaa ccttgctccg
     8221 agtagaacag ttcgttcttg tagcttccgt cttcgttttt tacccgcttg tcggtcatag
     8281 tcagagagat gccagcgttc aggtaagcga gttcacgcat acggttggcc aatatgtcgt
     8341 atttatattc cagcgtggtg aatatctcac tgtcgggttt gaatgtgatg gtagttccgg
     8401 ttcgatcggt agttcccgtt tctttgaccg gatagagagg tttgccctta tggtattcct
     8461 gttcgtaaac ttttccatta cggaaaattt gaacaaatag gcggtcggag agtgcgttaa
     8521 cgcaggaaac acctactccg tggagtccac ccgaaacctt ataagatcct ttgtcgaatt
     8581 ttccaccggc gtgcagcacc gtcaatacga cttccagtgc cgaacgccct tctttttcgt
     8641 gcatatctac cggaataccg cgaccattgt cggataccgt gattgagccg tcttcattta
     8701 tccacaccct gatgtctgaa caataacctg ccagggcttc gtcgatacaa ttgtctacaa
     8761 cttcgtatac caggtgatgc agtccttttt cgtcggtatc gcctatatac attgcgggac
     8821 gtttgcgaac agcctccaga ccttcgagaa cctggatgtt tttcgctgaa tagctttgtt
     8881 cgttgtttac aatttcttct tcagccataa gtatttatat taagtcaacc ggtcgcgaaa
     8941 agcgaccgca aaaaacgaac aaatgtagcg aaaatcccaa aaaccaccaa gaaacggaat
     9001 gtgtttttta atgctattcg tgaagaaaat accggtaaaa tttggatttt taggaacctt
     9061 ggcttatctt tgcatccaca aaatcgcgga gtggagcagt ggcagctcgt tgggctcata
     9121 acccaaaggt cgcaggttcg agtcctgtct ccgcaactag gtcagagggg ctgtttgaaa
     9181 acagcccttt ttttcggaca tttaccttgg acatttcccc tattaaatcc ctaaaagaat
     9241 tccgctcatg gccaaagcta aaaccatctt tttatgcagc aattgcggtt ccgatttccc
     9301 aaaatggatg gggaaatgtc ctgtttgcgg ggagtggggc agtattaatg aagaaataat
     9361 tcatcgcgac gcgacatcaa aacaactgac ttctcagttt aatgctgaaa aatcccaacc
     9421 tcaacttctg gccgaagtag aatttaatca cgaagcgcgt ataagtaccg gaggcccgga
     9481 gctggaccgg gtactgggcg gaggcctggt tcaggggtct gttgtcttgt tggggggaga
     9541 acccggcatc ggcaaatcaa ctctggtact ccaaacagcc atcaagatga cacataaacg
     9601 tgttttgtat gtgtcgggcg aagaaagttt gcagcagatc aagatgcggg ctgaacggat
     9661 taaaggcgat aatccccagg cttattttct tgcagaaacc tctctcgaaa atatttttga
     9721 gcagatcagc cggcttaaac cagaattact gattattgat tccattcaga ccttaagcac
     9781 cgaagtggcc gattcttcac ccggcagcgt tacccaggtg agggaatgtg caacagcgtt
     9841 gcttaaggtg gccaagactt tgggcatgcc gatcgttttg atcggtcata tcaacaaaga
     9901 aggcagtttg gcagggccca aagtgttgga acatatagta gataccgttt tgcttttcga
     9961 aggcgatact caccatcttt accgtatttt acgctcagtc aaaaaccgtt ttggcagcat
    10021 tgccgagatc ggtatgtatg aaatgcgcag cgatggtttg cgagaagtaa ccaatccatc
    10081 ggaactttta ctgtcacagc atcaggacgg cctgagtggg gtggccattg ctgccgccat
    10141 cgaaggcatt cgccctttcc tgattgaaac tcaggccctg gtaagtacgg cggcttacgg
    10201 cactccgcaa cgttccacta ccggtttcga tcttaggcgg ctcaatatgt tgttggccgt
    10261 actcgaaaaa agagccggtt ttaaaatcgc tcaaaaggat gtctttctta atattgcagg
    10321 gggcattaga gtgaacgatc cggccattga tttgtctgtg attgctgcca tattatcgtc
    10381 caatttgaac ctttccatcg ggaataaagt gtgtttggcc ggcgaggtgg gcctggctgg
    10441 tgagatcagg ccgatcaacc gcttggagca gcgtattatg gaagctcaaa agttgggttt
    10501 tgagcaaatt attgttccgg atttctctga atctgcaaca gacttgcgac gtttcaaaat
    10561 ccgcatagac aaggtgcgca aggtagaaga atctttccgc ctgctatttg gttgaggcac
    10621 ttggagagcg ttgtgttgaa gctttgccgg cttatttgat gaaataaaaa atagacttaa
    10681 ttttaccacc caatttttta aacaatacaa gaatgaaaga acagcggatg atcggttcca
    10741 ggattaaagg aatgcgcgaa tcaaaatcaa ttagtatcga agtcttttcg gaacgttcag
    10801 gcttgagcgt ggagcaaatt cagcttatag aaagcaacag tcagatacct tcgctggcac
    10861 ctctggttaa aatggccaga gcgctggggg tacgtttggg gacctttctc gacgacaacg
    10921 aagacctggg acccgtggtc agccgcaaag aagatcagcc ggccggcata agtttctcga
    10981 gcaatacggc aagttcccac aacgatttgc aattcttctc gctggctcaa aaaaaagccg
    11041 gacgcagtat ggagcctttt attattgaca ttcaatcgtc cacccaaaac ggttacgctc
    11101 tttcttctca cgaaggagaa gaatttattt atgtacttga gggagagata gaaatttttt
    11161 atggtaaaag agtgtttaag ctctctcagg gcgacagcat ttactacgat tccatcgtag
    11221 atcataatgt tcatgctgcc aacaattcag aggcccggat tctggccgta gtttataccc
    11281 ctttctgata tggaattaag taatcgtacc gtaggagagt tcctggaaca ctgggcaaca
    11341 accacccccg atcgtgagta cctgatctat tccgaccgag acttgcgttt tacctacaag
    11401 caatttaatc aacgagtaga taatatggcc aaaggcctga tggccattgg tgtcagaaga
    11461 ggatcgcata taggcatttg ggctaccaat gtccccgatt ggctgacttt tctttatgct
    11521 tcggccaaac tgggagcggt gctggtcacc gtcaacacca attataagca gcatgaactg
    11581 gaatatctgg tcgaagactc cgatatagag accttgtgca tcaccgaagg tgtgttcgac
    11641 ggcaattacg tggacatggt caataccatg ctgccggaac tcaaaacttc gcagaggggc
    11701 catttgcaaa gcaaccgttt ccctaaattg aaaaacattg tttacatcgg tcaggaaaaa
    11761 taccgtggta tgttcaatac ggcggaatta ttgttgctgg gccaaaacat cgacgacgac
    11821 gaactgaatg aaatcaaacg cagcgtaaac tgccacgatg tggtgaatat gcagtatact
    11881 tcgggaacta cgggttttcc caaaggagta atgcttacgc accacaacat tgtgaacaac
    11941 ggctatttta ccggcgagca tatggccttt acatccgaag ataagctctg tgtaccggtg
    12001 ccgcttttcc attgtttcgg agtagtattg gccaccatga atgttctgac ccacggttgt
    12061 acccaggtga tggtggaacg cttcgatcca ctggtggtgc tggcatccgt tcaaaaggaa
    12121 cgttgcacgg ctctgtatgg agtgcccacc atgtttatcg ctattttgga tcacccgatg
    12181 ttcgagatgt ttgatgttac ctcgctgcgc acaggcatca tggccggttc cttgtgtccg
    12241 gttgaattga tgcgcagagt aaccgacaag ctgcatatca cccatatcac cagcgtgtac
    12301 ggacttaccg agagttcgcc gggcatgacc cagaccacag taaacgattc tttcgaaata
    12361 cgctgtacca ccgttggtcg cagttaccct tttaccgaag ttaaagtggt agatcccgaa
    12421 accggcaagg aatgtcctcc cggcgtacag ggtgagattt gttgcagagg ttatctgttg
    12481 atgaagggtt actacaaaaa tcctcaggct acggcggaag taatcgacaa ggacggcttc
    12541 ctgcactcgg gcgatttggg aatagtcgat gaaaacggtt attatcgcat taccggccgt
    12601 atcaaggata tgattatccg tggcggtgaa aatatttatc ctcgcgaaat tgaggagttt
    12661 ctctatcacc tcgaagggat caaagacgtt caggtggccg gcattccttc cgaaaaatac
    12721 ggcgaagaag tcggagcgtt cattattctg cacgaaggtg tcagtatgga cgaagagtat
    12781 gtcagggaat tttgccgtgg caagattgcc cgtcataaga tccccaaata cgtctttttc
    12841 gtcgacagct tcccaatgac cggcagcggt aagatccaaa agtttaaact caaggatgtg
    12901 ggtttaaaac tgctcgaaaa gcggggtgat taagcaggtt tgttacgtct tctcaggaaa
    12961 accagggcaa ggccgaggat taaccagacc ggccagattt taaagaggaa aatcacagtg
    13021 tccactactc cataccaacc tttggtgaga gcttgtttca gtttctcacc aaagcggtcg
    13081 cgttgttcgg gtttgtattg gaaatctttt tgctcggtga gcgttagttg cagactgctg
    13141 taatccactt gatcgcttag gtatttgagc cggccggtgg tgctttcgat ttcttcttcg
    13201 agcccccgga tttcttcctg aatctgcagg atatctttaa cggttttggc ctctttgagt
    13261 aagtccttgt aacgactcaa gtaattgcgt ttgttttgta gacgggtttc cagatcgata
    13321 aactggtcgg ttacatcccg ggcttgaatg tctttgttga gtatttctcc ataaccaccc
    13381 ttttcgaggc agagtataaa atcatcaaaa gcggcagcgg gaatccgcaa cctcaggtaa
    13441 aaagtggcct cccgttcgtt gttgttgaag ttttcgctgg cgtaataagc cccaaatgga
    13501 agcaaaaggc tgtccacttc ggttttggct gcctgagccg attttacctt gatggtcatg
    13561 tggccgtctc gaatgatttt cttcttgtct gttgcttccg atccttgttt tacagcctca
    13621 tcggtgatag tgctttcgga agccgcaacg ttgcctgccg cgtaggtctg agcacctgcc
    13681 acacgccctt tgaacttgag atcttctctt gattcaaagg aaacgtcttc catcattaat
    13741 atttcgttat ctgcaacaag gtagttgtct ttttgttgac atgctccaag cagtcccggc
    13801 aacaggagca gaaaaagaat gttctttata tgattcattt cttttcgggg aataccaagt
    13861 tgattttgct taattcggta tcgataatac gaagttcgtc ggggctgagt ttcagcatgc
    13921 ctgcccgggc gttttcggta gcctgggcta cgttcctggc gccggccaaa gcaacggtga
    13981 tgcccggctg ttcaacggtc cagcgcaata ctacctgact caggctggcc ttgtgcgctt
    14041 cggcaatggg gcggatcctg ttgaggaagt tgttgacctg cacgatgttt tcgtccaaat
    14101 accatacctg gcctgtgcgg ctgtcgttgg gatcaaattg ctggccgggt ttcattttgc
    14161 cggtaagcaa tccccgttgt aaagggctgt aagccaaaat gcccttgtta tgcttaatgc
    14221 aataaggtac agtttcatcc tctatccccc ggcgcagcat gctgtacggc aactgatccg
    14281 atgccaggtc gataaaggca gccgcttctt ccatttggtc ggcagaataa ttgcaaacac
    14341 cggcataacg cacttttcct tgttccatga ggcggctgac ggcttccatg ctttcgccca
    14401 cgggagtggt gggatcgtac cagtgtatct gatagagatc tatgtaatcg gtgcccagca
    14461 atttaaggct ggtttcgcat tcctgaataa tattctcttt acgggcattg cggtaaatgt
    14521 gtaccgtttt tccctcgttg ctgacgctgt caaaatagta ctcgccttcg gtcgtatccc
    14581 agcgcagacc gtatttagtc aggatttgta ctttgtcgcg aggaagaccc ttgatggcct
    14641 ctcccaccag ttgttcgctg tagcccatac cgtaaacggg agccgtgtct atgctggtta
    14701 cgcccatatc gtacgaagcg cggatggcgt tgaccgcatc gttctgtttg gtgcctcccc
    14761 acatcatacc cccggcggcc caggctccaa aagtaatgac cgagagttcc agatctgttt
    14821 tcccaatttt tctgtattcc atagtgattt tgttttgttg ccctcaaaga tataatttta
    14881 ttccggccta tcattatctt tgtaaagtat gaaaggtttt gcagccatcg atttcgatct
    14941 gaggcatcat tatgctttgg ccgatgcaga agcttgtgcg gcgatagagt tgagattatt
    15001 caaaccactt tgaataaata gactaaatca cgaaaaatgt tgttattgaa tatattatga
    15061 agctcagatc acattttacc gggctgatat tatttgcctt tttttctata gcgcttcaag
    15121 ctgccgtgcc ggcttttccc ggagccgagg gagccggtgc ctataccacc ggaggccggg
    15181 gaggaaagat ttaccgggtt agcaatttaa acgattccgg cccgggttct cttcgcgaag
    15241 ccgtgcaggc cagaggtaaa cgcatcgtta tttttgatgt ggcgggcatc attgatttgc
    15301 aatcgaccct gaaaatcaca aacggagaca ttactattgc cggccagaca gcccccggca
    15361 aaggaatatg tttgagaaat tatcccctgc aggtggctgc cgacaacgta attatccgct
    15421 ttattcgcag ccggatggga gaagaggccg gggtggaagg cgatgctatt tggggacgtt
    15481 accggcaaga cattataatc gatcactgct cattcagctg gagcgtggat gaatgcgctt
    15541 ctttttatgg aaacaaaaat atgaccatgc aatggtgtac catttgcgaa agtcttaatc
    15601 tgtcggtgca tgtaaaagaa aggcacggtt acgggggtat ctggggaggc tcgccggcca
    15661 gtttccatca caacctgata gtgcaccaca ccagccgtac gcccagactc agcggaggcg
    15721 atcccgctac cgaaatggtg gatgtacgca ataacgtctt ttacaactgg ggccccacca
    15781 acggaggcta cggaggaaca ggcggcagct acaattttgt gggtaattat tataaacccg
    15841 gccccagcac ggcggtcaag gattttctct gcaatcgtat aatgtacacc ggaccttcgg
    15901 aaaacccgga ttcagcgggg aattatattt ggggcatttt ccattttgac gacaattatt
    15961 ttgacgatag ttgtcccgat ctcagcgaaa cagccattaa taattgttcg agggtgaatt
    16021 ccaataatta cgttggttta catctttcta ccagcaccaa tctacccgga gggaccaaat
    16081 acggcataca gtcgtccact cccttttcct gtttgcccgt gagcacccat cagccccagg
    16141 atgcctacga caaggtgctg gaattggccg gagccagtct gaagcgtgat aagcaagatc
    16201 agcgctatgt agccgaattg atcgacggca tctataccta cgaaggcagc aggggcagca
    16261 ccaacggcat tatagattca caaaacgatg tgggtggctg ggatacctat accggaagga
    16321 acaccgaagg cgattccgac ggtgacggca tgcccgacaa ctgggaagac atccacagtc
    16381 tggatcctgc cgatccttcg gatggtccgg cttacacttt gtcgcaacag tacaccaatg
    16441 tagaaatgta tttaaacagt ctggttgcat ctattatgga agccggtctc gagggggccg
    16501 agtcgaatct acgatcggtt gataagcccg atgacacccc ttcctactta tggaccgaag
    16561 ccggtctgtt ggttcccggg gctgtcagcc tggaacttta tacgatgggt ggcctgctgc
    16621 tcgccaaggc gcagggaagt ttactggaaa tctctcccac acccggcttt ttcctcttaa
    16681 gggttaaaga tgctgctttc cgggagcata gcatcagtct ggtaaaatga aacataacca
    16741 aattaaataa atcagaatgt tacaaaaagg cgaaaaagct cccgacttca agggagtgaa
    16801 tcaacaaggc gaaatcattt ccctgagcga tttcaggggg aaaagagtga tactgtactt
    16861 ttatcccaaa gacaacaccc ccggctgcac ggccgaagcg tgcagcctta acgacagccg
    16921 gtcgtatttt ttggataaag gctttgttat aatcggagtc agtcccgatt cagtggagtc
    16981 gcatcagcga tttgcggcca aatacggcct gtcgttcaac ctgatttccg atcccgaaaa
    17041 agccattatt caggcatacg gtgcctgggg cgaaaagaaa aactacggca aaacctatat
    17101 gggcctcctg cgtaccacct ttgttatttc ggccgatggc attatagaag aagttttccc
    17161 caaggtcgat accaaaaaac acgctgagca aatcatcaag gcgttgggaa tttaaatcct
    17221 atttccgcat ccaaaccatc gactcaggac tgcgtcgtcc ttcttgtacg ggctggattc
    17281 tgttcatcct gagataatta ggtatagcca gcaggtctga gccgtcggcc cattttcctt
    17341 cgatactgac tatgcctttg aacatatcgc tgcgccagac agctttaaag ggctcactgc
    17401 ccacggcttg gttgatgtct ttgttgtcgg ctctttccac attgtaaatc attggcccgt
    17461 agcgaagagc aaccagcccg cttagagctt caaccttttc gtcggccgtg atttgctgca
    17521 cttccagagg aatttccagg tctatgcgat cgcccttttt ccagtcgcgg gcaatggcta
    17581 tataacctct gtctgccttc atttccacag cttcgccgtt tacactgagt gatttgagcg
    17641 tattcagttc gggtaccgaa gtatacagct cactggtttt gcgattgggt acccgcacat
    17701 aaaccgtaaa atgtttgtct tctttgggat tgacgatgat agaaactgcg cctttccagg
    17761 gataatcggt ttgctgaacc atttcaacat ctgtgccggc taccctttct acattgatgg
    17821 tgctgcctac gtacatatta acgtagatac cgtcttttcc ggtaacgtaa gtccaggtgg
    17881 gaatcatcag cagggtgcgg gggatattac ccacgcagca ggggcagaca tgccagggat
    17941 cgcgcgcatt gtaggccgta agcgggttgg tgtaataaaa acgcttgcct tcgaaatcga
    18001 tcgaacccaa taaggcattg tacatggttt cttcgtagag atcggcgtat ttggcgtcgt
    18061 gatatgcgat gttcattttg tgctggaaaa atatcagccc gcagctcgag caagattcgc
    18121 aataggcgtt gttacgcaga gagtagttgg ggccaaagcc ttcggaagtt tcgccgctgc
    18181 ccacaccgcc ggtaacataa tattttctgt tgaccatatt gttccacaag gacattacgg
    18241 cgctttgata atcgatatcg ccggtttcgg cggctacatc cgacataccc gagtaggtgt
    18301 agacggcccg cacagcgtgt ccaacggctt cgtattgctg ttgtacgggg aggtggcttt
    18361 gatcgtattc gccgccctta tcgcgcatgt cgagcaggaa tttggccagg gcaatgtagc
    18421 tgtcgccttt gttgttgcct tccatgtcgt taacaaaacg gccaaagcgc accagggcct
    18481 gttccatttc ctgatgtcca tcgtaccatt caatctgatc gggacccggg ccaatgttct
    18541 ttacccaaca gtcggccagt tttttggcag catcgtacaa acgagtgtct ttgccttcgg
    18601 tcagggtgta gtggttgata gcggattcta taaaatagcc ggccacatag ccttcgtgat
    18661 cgctccggtt acgcggagac cagcggccgg tccagcgggt ggtgtcgcgc aaagtgtagg
    18721 ctgtgtgcag gtagccgtcg ggttcctgag ccgataaaat gatgggaatc cacctttcca
    18781 gagctttctt catcttgttt tgggcggcaa tgatctcttt atcaccttga ggatcaacca
    18841 tcagggcaat gcacatagat tccactgtct gatgaaccca ggcattggaa aacacataac
    18901 caaggtgccg gccgtgaggc tcaccgcgca aggctttggc cgcttcgaca aagttgtcca
    18961 gtccgccttg tccctgagtg agatcacggc gttcgcattg gtcaatacac caagggatcc
    19021 aattagtgat taagaccttg gctctttcgt tccaaagagg actgttgatt ttgtaacgct
    19081 tggtgtagac cacatccaaa cgttcttctt ggggaggata atgtgcaatg agttccaggc
    19141 ttgatccgga tttcattttt cccgattggg cagagagcgt caggatgtat ttgccgggct
    19201 tatccacgct ggctgtggtg gtaagagctt ttggatcggc aaaagatacc ttgcccggtc
    19261 ctttggcgct ccaacgtagc tttttaaccg gatcgaccgc cttgatttcg gcattcaggt
    19321 aggtattacc gcctaccatt acgtcacggt ctataccggc cgtaaccata ggggcaacgg
    19381 ccggagagtt gagcgatttc attactctcc attcgagtaa agtgctcgga tagcgaggca
    19441 gagagtccaa ttccagccgc agcgcggtag tggtaacttc gtcaaaaacg gttacattga
    19501 actgattgtt ctccagtccg taaccttgtg cattgctgac gggtacaaat tgctgtccgt
    19561 cccagtaact gagccggtgg gcctgaggca atttggccag tccttcgtaa ttgaaccaat
    19621 aaagttgcac ctcgtctatg gtgaccgggc tggcccaacg atattccaat tgctggctca
    19681 ctaattggtt gtagcgtctg tattggggtt gattggcttg ttcaacggct tgcatcatcc
    19741 ggttccgttg ctgttcgctc agttcgttag ggatttcacc atcgttgagc ggatgcgttt
    19801 ctgcaccacc cctttgacct tgaaccttgg ccagtacggc caggttagct ttttctttgg
    19861 gtctgtcttt gcaggagttt atgccaaagc ttaagaacac tgccagcagg gcaggcagcc
    19921 aaatggagtt tgtgaaatgt tttttcataa tggttgtgaa tttttataga gtgatttatt
    19981 taagtcagaa gaacgcccgg tattttgaag gcgttgataa ttaatcatta cgcaagttac
    20041 atgcttttat cgaataagtt ctgcgttttg tatgcaaaaa ttcctaaaat cttctcaaag
    20101 tataacacta tagtccaagt aataagggag tttagtgcaa aaaccggttt ctctacagaa
    20161 aattcagacg tgacacccgg accaacatca gggctcaacc acaattgttc tgaacgtacg
    20221 gcttccgccg tgtttgagct gtagaatata aatacctgct gccagatggc tgagatcgag
    20281 ttcggccttg ctacccttta aggtgtaagt agccactttg ttgccgtcga ggctgtagag
    20341 gtcgatttga ccggttctcc ggtgatcgcg gccgcagtcg aggtaaagga ggtcacgggc
    20401 gggcataggc catattttca gttcgtttat ttcgctgccg gaaatctccg gtagggcgtc
    20461 cgggccattt gccgccagtg cggctacttc atcggtcgag agagggtagc tatagaccct
    20521 aaagtcgtcc agttgtcctt tgaacaagac actcacgtgc tggcccctgc ccaggtagtt
    20581 gattatcggc cggacatctg ccggattcag gctgaattcg gtcgattgat ctgctatttc
    20641 tccgtttaca tagaggcaag ccaggctgtc gctcagggta acggccacat ggcaccattg
    20701 attctttggc agcgcggcag cttccagtct ttcttcgcct cctttgtttc ggatggcgaa
    20761 acgcatttta ttaaaaacgc ctatcttggg ggttaggtaa aaatagctgg tttgatcgta
    20821 aagaaaatcg aaaatacgtt gccagctatc tcccccttgc cagtttaccc ataaagccag
    20881 gctgagctcc gggtggttgc ccagagtgga cggcagttgc acaaagcccc gggttccgtc
    20941 gagtgtcagt gcccgggagg cattgatgcc ctccacaaaa ctcaggcctt cgagcccctc
    21001 gccgtgattc agatgtggag tgttgtctaa ggtgttgtct tcgaagctga gccatgcaag
    21061 catgtccctg ccgccgcttg ccaccgccgg caccgtatcg gaaaaggcgg aatgattcag
    21121 cgaacggtct actgctttaa cagcgtaata ataggtcttg cccgcttcga tggaatgatc
    21181 cacaaaggaa gtgccgttga tgtttctggc caaagtctga aacgatccct gcaacgaatc
    21241 ggagcgataa atggtgtaac cggccaggtc tgcgtccccg acggcagccc aatcgagacg
    21301 caaggaatga gaatttgcgg cggcacttat ttctgcaggg gcggcaggcg gattaaattc
    21361 aatggctgcg cccaccggca ggaagcgcca ttgctggttg gctgagccgt ttttggtgcc
    21421 ctgttgaatg ttggcggcat tggcagtgga ggcgttttgt atttgcaaac aatgcgagct
    21481 gtgtcggctg cggatgtaaa accagccgtc gccggcgtat tccaggtacc actgttggtt
    21541 ggcacctttg gcatcgtccc aaacgatgat gttgcccccg ttattgagag accagttgta
    21601 aacatccaac gattttccac tgtgattggc aataatgctg tgatagctga aatcgccccc
    21661 aatgcgggga tctaccgggg ttacaaccca ctgttggtag gctgccccgg tgtaggtatt
    21721 ttgacgtacg ttgctgccag cgcttgtgga gccgttttcc acctccatta ccttggcgct
    21781 gttccggttt accaatacat aacgtccgtt gacgggttga ggtacatctt ctccccagtt
    21841 tatgttgatc actctttcgg cattggtttg accttgctga tagcctgtgc ctcccgggat
    21901 ttctattgta aaagctcgct gggggccatg tccgtcgtaa aagacgtcgc gttcggaaga
    21961 gataaaacta taagaagtag ttgcggcctg ccgctcggag cttcctacaa aggcctgcac
    22021 tttgccctcg ggagagcggt aaaccgttgc tgccgtccag ttgggcctgt gttcggcata
    22081 ggccaggcgg cggccaaagc tcgctttgac gaattctccc cgggccagtt cggcggtgcc
    22141 ccaccaaatg cccgtctgca taccgtattc tacgccgacc atggcttcca tcacattatg
    22201 caattcgtcg ttggtggcgt gatgtccgtt ggcgcgcacc gttctgaaaa acgaagcgta
    22261 attatcgaaa cttccggcca attgatgggt gtttccttcg tcgagacggc ttttcagaga
    22321 attataccaa ctcagtgcct ggtctgtgtt cagggtattg cctccgctga tgcgaatgct
    22381 gtcgaagcgg ggattcttgc gcaattcgcc tgcaatgttg aaaaaatcgg tcaagctgcc
    22441 ctgcccccaa ccgtaatccg gttcgttaaa gggagcaatc gatactatct gacgtcctct
    22501 ttcctgtatg cgacgggcgg tgacatccat cagttgtgcc cagttttggg cattgccggc
    22561 ataccaggga tcaacgctgg ggtgatcgca attcaaggct accttggtat ggctgcctgt
    22621 cagattaata agattgaggc gattattgag atcggctact tgttgcgact gcagatctcc
    22681 gttcaccagg ggcatagtgg gttgaaagga agccctgacc aaataaatat tttcgctgcc
    22741 cataaaagca actccccgta taatgttgtc gcggctgggc caggcggtgt cgagccccca
    22801 gagtattggc gtgtgttttc cgctgtccga taaattgaaa ggaactattc tgtcggcaac
    22861 aggttgcgga tgaatgggat tggtcgcggg taaggattct gcctcggccc gggtatggac
    22921 ggcggtccct tcaaatcgcc ggtcggtttg agtggtggag ccggtggctc tgtgttggaa
    22981 tgttccctga gcatcagaca cagggcatgt aaaaaaaagt aggcccaggg aaaatataag
    23041 tttgtgagaa aatataagtt tgttcatggt tgaaagactg gctgataaaa aataagcgca
    23101 cactcttaca aagatggagg cattatttta cacctcaaaa tgtttgatgt tttttatgaa
    23161 taaattttga aatttattac gacatttgtc gatactatga aaacaaaatc tacacttgtc
    23221 cgctttgccc tgggtggggg agcgacgtta ttattcttat ttgccggtct gttcaagacc
    23281 ggggctcagg atcccagaga aaggtattat tattatgaaa tcttagaacc ccatcacgcc
    23341 cctaaacccc aggtggaggg ttttgctacg atgagggtga acgaggccct tgaccgggga
    23401 ctgacagcaa cacgggctca ggacgggcag tcgctctacc tgagttggag actcttggca
    23461 gaggaccctc aggatgtggc ctttgatgtg attcgtgtgg aaggtcaaaa aaatcaacga
    23521 ctcaacaagc ggccgattac agccacctgt gattttattg ttgagaatcc tccggctgag
    23581 gctgtttaca aagttgtgcc gcgaaacaac cgaaagtcgg tgaattctgc cggagcaaag
    23641 gctgcagcta atccgggcac ggctgcggcc agcctggaca agactaaagg tgtcctaaat
    23701 acaggcgaag gcagcatcgg catccggatg gaagatttac cggcctttcc ctatctaagt
    23761 attaaactgc gagatggcgt caggccgggc aagatggctc tggccgacct caatggcgat
    23821 ggcatttacg attacattat ccgtacaccc gattcgagcg ttgatcccgg tatgccgggc
    23881 gatttggcgg gaagaaccta tcaaattgaa gcttacctga gcgacggcac ttttctctgg
    23941 tctttcgacc tgggtcaggg tatagaaccc ggtgtgtggt attcgccttt tatttgttac
    24001 gacttcaacg gagacggtaa ggccgaggtg gccctgaaaa cggctcccgg cgatattgtg
    24061 cgcaacgaaa aaggccgggt ggactatggc gacgaatacc tctcggtgct cgacggcatg
    24121 acgggacgcg aaatagaccg tgtcgactgg ccggtgagga atttccgtta tggtgatctc
    24181 aatcgtcaga gcagacatca gataggtatg gcttttttgg acggcaaaac accttatatt
    24241 ctggcagcca gaggcaccta taaactgatg accgtggatg cctggatgct ccggggcgac
    24301 aaactggaat tggcctggag atgggacggc gacgaagaaa acccggtggt ccgcagcatg
    24361 ggtgctcaca acatgatttg caccgacgtt gacggcgacg gccgagacga agtggtcttg
    24421 ggttcctgtg ccctggacga caacggcaca ctgctctggt cgacgggtct aggtcatccc
    24481 gacaaaattt tcatcagcga catcgatccc aatcgccccg gcatggaaat gttgctctgt
    24541 ctcgagccct ggcacgataa cggaagagga gtttgtttga tcgatcccaa agacggctct
    24601 cccatttgga acatagggca taagacttat cacgtgggcg acggcatggt agccgatttc
    24661 gatccgtcct ctcccggttt ggaatgtttt gcttcggaag atcannnnnn nnnnnnnnnn
    24721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24781 nnnnnnnnnn nnnnnnnnnn nnnntagccg atttcgatcc gtcctctccc ggtttggaat
    24841 gttttgcttc ggaagatcaa aaaggcggca gcagcgacaa atatttattg agtgccgatg
    24901 gccggataat aggcaaaaac gaagaagtgc ctccttgccg taactgggcc tggtgggacg
    24961 ccgatttgtt gcgcgaatac atcagcgctc agttttaccg cagccccgag ggccggttcg
    25021 gcaggaaatc gggtattgga aaatggaaag gagaaacatt attcggagaa atggagggta
    25081 gtattctgat gatagccgat gttttcggag attggagaga agaaattatt accgccttgc
    25141 ccggggaatt gcgcgtgtac actaccaata ttccggcccg tgatcgccgg ataacactga
    25201 tgcaggatgc tctttatcgc aattacgtgg cgcatcgctc catgggttac gatcaggcac
    25261 cggtgccttc gttttatttg ggagaataaa ggctgaaaaa gctcaggttt ggtaaaccaa
    25321 cacagcctcc tgaatgtcct ttctgtcgtg attgccggaa agtcatttta gttttacgac
    25381 taatgaataa tgaaatgcga tcgttctctt tgaacttaaa aatagtcttg ctgctgattt
    25441 ttgcagggca gtctataggc ggaattgcac aggtgattga acctgaatcc gataaagatt
    25501 attacctgat ccattccggt gcttgcgttt tgtcgagggc cgacgacgga cgcgctgtgg
    25561 tgcagactgc cggtgcgacc ggaacccagg tgctgaactt cgtagccgat ggcaggggct
    25621 attataccgt caagctcaag gatcgccggg aatacatggc gctctcgggc agttggaaca
    25681 cttattttgt atccgattcg gcttccgatg ccgccaaata tgccgttgaa gaagtgcgga
    25741 aaggcgtagt acgtttgcgt tgtaaggcca atcggaaata cctgggcaca gacaacagta
    25801 cagccggctc acatgtctat tccgacaaga gtggctcgga cgaaaggcat tactggctga
    25861 tctccgaaga aatcagggaa ttccccgtcg acaccgacag cctgtattac ctgatcaatc
    25921 cctccgacgc cttcggcaag actttcgaag gctggggtgt ttcgctctgt tggtgggcca
    25981 atatgtgcgg ccgttgggag gaaaacaaaa tagacgaaat catcgactgg ctggtcagtc
    26041 ctgagggttt gaattaccgg atattccgtt acaatatcgg cggaggcgac gacccgctca
    26101 accgcaattg cgatccccac catatggcct tgggcaaggg tctgcgagcc gagatggaag
    26161 ggttcaaaga ttctgccgac gctccctacg actggagtcg cgatgctgct cagcgcaaaa
    26221 taatgcttaa aatcagggaa aaacgtcctg atgcagtatt cgaggctttc agcaattccc
    26281 cgccctggta tatgacctac agcggatgcg tggcgggtaa cgagccggcc ggccacgaca
    26341 acctcaaacc cgaatattac gaggctttcg caacgtattt ggtagatgtc tgccgttttt
    26401 ataaagactc cttcgatttg gaatttaaga ctctggagcc ttttaatgag ccggttaccg
    26461 attattgggg acgcaacggc ggacaggaag gctgtcattt cagcactgca tcgcagattg
    26521 cttttttgcg ggtgctggct ccgaaattgg aggcttcggg tttgaatacg gtgatatcgg
    26581 cctcggacga atcttatacc gatcagtcgg tgagagattt tctggcttac aggaacgggc
    26641 aggttatagc gttgacggga caatggaaca cgcataccta ttcggccaac aaccattcga
    26701 gggcagcctt gcgtgaactg gcctcggcag aagacattac tctttggatg agcgaggtgg
    26761 gcgcgggcgg cagcggcttg gccggtaact tgagtctggc gcaaaaactc atcgacgata
    26821 tccgttacat caggcccgaa gcctggatag actggcaata cgtggaagaa aacaacgacc
    26881 agtggtgtac cgtcagaggc agttttcaga atcaaagata ttggaaggtc aaaaactttt
    26941 acgttcgacg gcaatttacc caattcattc aagccggctg gcgtttcctg aatgtgccca
    27001 acaacaaaat gctgtcagcg ctgagccccg gaggcgattc tctgattgta gtagctctga
    27061 atacgagcac agtgcgtccg gcttaccaca gcatagattt aagcctgttt gacgaaatct
    27121 accggggcat caccatgagc aggacttcgc aaagcgaaaa caatcagaca gtgaacgatt
    27181 ttgtttggcg ggattcctgt ctgcagtttg tgcttccgcc gcaaagcatc ggtacgctga
    27241 tgatacccgt tgcctttact ccggaaacga ataccgaacc ggtaagcggc cggccctacc
    27301 tgatcgcatc gcgctgttcg ggtctgatgt tgcaggctga gggcgatgga gtgctgatca
    27361 acgattaccg ccctgccgat tcctctcagc tctggctttt gcaaaaaacc ggcgagggtt
    27421 atactatcgg caatttgcag ggactccggc tcaacgacga tggtagttat tttttaaaga
    27481 ctacttcgac ctttgatccg aataatcagc tttttgacct ggacttgcgt aacgataatt
    27541 ttttcaaaat ctcttcgctt tctaccggga aagctctcga tttacaaggg gccaattata
    27601 cgcccggtac aagggtgggc ttgtacggtt acgatgaagg gggtgcagat aaggtcgtta
    27661 cacgtcaatg gatgttttta cgtccgccgc gccgactctc cgacttcaat acttctctgt
    27721 tcccgccgga gcatgagacg gctttggaag ttcagttgag tgttgctccc ggaatggtac
    27781 agcttttttg cccctctttt gggcatgacg gaaagaccta ccgtcttcgc ctcttcaact
    27841 tgtcggggat catggttcga gaaagccgtt tgaattcagc tattacccgt ttgtcattgc
    27901 cggagggatt ttatctcttg agtatagatg ccggagccga ggaaaagatt tttgttcaaa
    27961 agatcattgt tccctaaata aaacaaggcc ccggcaaaca taattgcagt gtgtttttgt
    28021 ttattaaaaa acacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnattaaa
    28141 ccagaatgag aactccgaat ctctattgca ttaaaaccgg tttgccttta attctgcttt
    28201 gctttgccgt tttctgtggc agttgccggc aaggctccac aggagggagc cgaacggagc
    28261 ttctgtttga aagggaaaag accactactc tttggctgga taccggcgag gcagaggtgg
    28321 tgtatacggc tgtggatttg tttcgcaggg atgttcagct tgtgttcaat gcggatttaa
    28381 agatagttga ggataaaaaa gacgcggata ttgtgcttgc tttttccggg ctgaaaaggc
    28441 ctgcttcctc cgggccgaaa aagttttcgc cttcttggct gaaaagatct gcagttgccg
    28501 ggccaaaaaa actcgcattc ttcaagtgcc ataggtcttc gttctccaaa ccaaacagcc
    28561 ggggagggga agcctttaga atccgtttgt gcaaaagcgg ccggaaacct caactgatcg
    28621 tgagcggcag tgagccacgg gggctggctt atggaatatt ggaactttcg aggatgatcg
    28681 gggtgtcgcc ctggcattat tttgccgatt cccgcccgaa agaacttgaa tcttttgaat
    28741 tccctctgaa aagaatagaa cagcaagcct ccgtgaaata ccggggtata tttatcaatg
    28801 acgaagattg ggggctgatg ccctgggctt cgcaaactct ggctccgcag caaggcaaag
    28861 gagtgatcgg tccggaggct tacgaaaaga tatttgagct tttgttgcgt ttgcgcgcca
    28921 ataccatttg gccggccatg cacgagtgta cccgcccttt ttaccaggtc gaaggcaatg
    28981 cccaaatggc ccgtaaatac ggtattctgt taggaagttc ccactgtgag cccctggctc
    29041 gcaattctgc ctctgaatgg gatatggatg ggagcggcga ctataatttt atgactaatc
    29101 cttcgaatgt aatccaatat tgggccggac gtttgcagga acttagtccc ggagagaata
    29161 tctttaccct cggaatgagg ggtaagcacg acggcctgat gcagggggtg aaaacgctcg
    29221 aagagcacaa agcggctctg agccggatta ttccggttca gcaggacttg ctcaaacagt
    29281 atattgatgc cgatatagaa gcgattcccc aggtttttat tccttacaag gaagtgatgg
    29341 aggtctatga cgccggcctg gaggtgccgg atcatgtcac tttggtgtgg tgcgacgaca
    29401 actacggtta catccgccgg ctgtcgaacg cccgggaaca agaacgcagc ggcgcttcgg
    29461 gtatttatta tcatgtgtcg tattggggac gtccgcacga ttacctatgg ctggcaagca
    29521 cttcgcccgg cttgatttat tccgaaatgc tgcgggctta taagcacggg gccgatcgtt
    29581 tgtggatatt gaatgtgggc gacatcaagc cggccgaata cctgatggaa tatttcctgg
    29641 acctggcctg ggatatcaat gcggttgaca atgattttgg gcatttgcag cgtttcatgc
    29701 atagggaatt tggcgcagaa caggcaacag cgctgagtga agtctatgag cgctattacc
    29761 ggttggctac cattcgtaag cccgagttta tgggctggag ccgggtagaa gaatccggtt
    29821 acggccgggg aggaaaaact cctgtccgcg atactgaata ccatccggag tttaacaagg
    29881 aattacaaca tcggctggag gcttatcgcg aacaggaaca gcgagtggcc gaaattcgtc
    29941 tccgtattcc cgagcagcaa ttgtcctgct tttttcaatt ggtagaatac cccattcgtg
    30001 cggcttcact gatgaatcag aagtggctgt atgctcgcct ggctcgttat tacagcgaca
    30061 gtcgtccgga gcttgcccgg ctttgcgccg ccctgagcct gcaggcctac caaggcattg
    30121 agcagttaac ggcaacatac aattcgctgg aacagggcaa atgggagagg ataatggatt
    30181 tcaggccccg ggaacttccg gtgttcgata agccggtgtt taataatccg gaactcgatg
    30241 gtcctgaaca gaaaagctca gagtttggaa aattcttgtt cgaaggcccg gagttcgaaa
    30301 agctgtttga ccatacactc gaaaacaaca ggcgattgta tgattccctg atttcacaaa
    30361 cggtccaaag tgccgattcg gcctcccttg tcgataattc aaggcctttt gtaaccgcct
    30421 gcaacgccgc ccgggctcgg agtgttcgcg ggaaagtccg tagcatccgg ggtctggggc
    30481 atagttttga agctgttcaa atggcgcagg gcagtagtgt gaattaccgc tttgacttac
    30541 ccgaaagcgg ggaataccgg atcgtaatag ctgccgttcc caaccacgac gtggatgggc
    30601 aggggatgaa aatcagggtc gctgccgacg gcagggattt gggcgaattc gattataaga
    30661 cgcgtggtcg cagcgaagcc tggaaacaac aagtcctacg cggacaggca ttaatagaaa
    30721 tccctgcccg ggaaatggaa aa
//
LOCUS       DAOSTD010000014        30138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1683_length_30138_cov_16.402054, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000014 DAOSTD010000000
VERSION     DAOSTD010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30138
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1683_length_30138_cov_16.402054"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..>1972)
                     /locus_tag="PLI37_01695"
     CDS             complement(170..>1972)
                     /locus_tag="PLI37_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOQ57765.1"
                     /translation="EYGYRDAVIVEDTVYRFDDNTVNVYLKVDEGRQYFIRDISWVGN
                     TLYSSEELNMRLQIEKGDVYNQKRLNERLHQSPNEDAITDLYMDRGYLFFQAIPVEVS
                     VAEDSIDLEIRLSEGQPASIKRININGNDRLYEHVIRRELYTKPGELFNKAALMRSAR
                     EIAQTGHFDPETMGINPIPNEDGTVDIDLNLTSKSNDQVEFSLGWGSTGIVGSIGLKF
                     TNFSMRNLLRLGEGVLPQGDGQTFSITGRTNGQYYQSYSFSFMEPWLGGKKPNSFSFS
                     GYYSYQTGVSNRYYENYYSNYMMHYATYGNTYDYGDYYLTEMDPTKYIKMWGLSAGLG
                     TRLTWPDDYFHLFSEFSYQHYNLENWMFFLISNGRSNDLSLNFTLSRNSTDNPLYTRS
                     GSSYSLSLGITPPFSLLDNKTDEDYKKMSSQELYHWIEYYKVRFRSRTYTPLNGNRKL
                     VMATRFDIGLLGAFNKYKRSPFGTFYMGGDGMTGYSSSYAYDIIALRGYGNGDLGVGW
                     LYQRAGLELRYPLLMEQSATIYASAFLEAGNLWSDFKHYSPFELKRSAGVGVRIFIPI
                     VGLLGIDWAYGFDPVGTETKAHGGEFHFVIGQEF"
     assembly_gap    1973..2072
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2073..2515)
                     /locus_tag="PLI37_01700"
     CDS             complement(<2073..2515)
                     /locus_tag="PLI37_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOQ57766.1"
                     /translation="MAQEEALPEIYYSYPRELKIADIAVEGEGIDNYDDYVLIGLSGL
                     SVGQIIRVPGSETSEAVKRFWRHGLFSDVKIIADKIEGNEIWLRIVLSPRPRISKINY
                     NGMKKSEIEELEPRLNMIVGNQITPNIIDRAEILIKKHYDGKGYK"
     gene            complement(2595..3338)
                     /locus_tag="PLI37_01705"
     CDS             complement(2595..3338)
                     /locus_tag="PLI37_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOQ57767.1"
                     /translation="MSGLEKIDKSTLPKHVAIVMDGNGRWAKGRGKDRIFGHTEGINS
                     VRAVVEAARELGLSYLTLYVFSTENWNRPQKEVNALMRLMTETIDRETDDLIKNGVRG
                     LVVGDLDRLEKDIQDKVQQFMHNTAQGNELNLVLALSYSSRWEIMEAVKSIAKGLSEG
                     RILEQELDEDCFSKHLCTAGIPDPDLLIRTGGEIRISNFMLWQAAYAELYFTETAWPD
                     FRKEAFYEAIIEYQSRERRFGKTSEQLTK"
     gene            complement(3340..4026)
                     /locus_tag="PLI37_01710"
     CDS             complement(3340..4026)
                     /locus_tag="PLI37_01710"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOQ57768.1"
                     /translation="MTGRRAFFSICLLLCSAAMYGQSYRIEIGGLGGLSFYTGDANQT
                     KLFEKVHPAYGVLMRYHLNRRFSIKGNLLSAGISGTTTDKLEQYPGAEALDFSRRVLD
                     GGLQFEFNFYEFGAADYYPGAGNVSPYVLCGIGLTGYKTDKNALTANIPLGLGIKLKL
                     PARFNLGLECTYRMSFSDKLDYTPQSNLFHLDDPWIAQSAWNKNKDGYFVLAFYLSYD
                     LFYIGSKCYK"
     gene            complement(4093..5433)
                     /locus_tag="PLI37_01715"
     CDS             complement(4093..5433)
                     /locus_tag="PLI37_01715"
                     /inference="COORDINATES: protein motif:HMM:NF039698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6242 domain-containing protein"
                     /protein_id="HOQ57769.1"
                     /translation="MNKLLYSIGKKVCLLVLLSPVLLGVSSCHSFEDQSFSLANVQHF
                     GFDSVPGITRYVFYVDNFAGLIYNNDSLPYQTRIDSLVPSIVFFSSNGNVFINDTMLW
                     KPGDVLDFSRPLSLTNTSDDGKHQKKYRIRVNAHQINPDSMVMNVLSSRFPTIAARNK
                     TISYNGLFKAFFAPDTGSVISYTSTDQGLTWSADAPVSGLDDTIFIQSLTEFKGQYYL
                     LGKQGRLYYSADLNQWSGSADSTKVVSLFAKLEGRKFLSEDALVGIVADNSGDLRFAR
                     FEGESEGWECGTAVPENFPLCEYALTKYKTVTDVPYISLLGGKNASGALNGDSWSTMD
                     GLYWIKVNTKPLHIEPRQGASLFHYDDKMFYYGGLDSRGLCDNTLYVSGNNGVTWSAA
                     DTLLQFYSIDGGLAYQDIFVEEGKYIWIFGGESGSNISNKVWKGNIHRKLFPGK"
     gene            complement(5533..6669)
                     /gene="ribD"
                     /locus_tag="PLI37_01720"
     CDS             complement(5533..6669)
                     /gene="ribD"
                     /locus_tag="PLI37_01720"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOQ57770.1"
                     /translation="MVNNLFTKIMHFSLLHNTSADKNPEALYMQRCLQLAANGRISCS
                     PNPMVGALVVHQNTIIGEGWHHSAGKAHAEPQAIKSVKNRELLKESTLYVNLEPCSHF
                     GKTPPCTDLIIEKKIPRVVIACPDPFPGVCGKGIAKLKAAGIEVELGLEAEAANYLNK
                     SFFCYYQKQRPYIVLKWAQTADAYSDYIRQPGDGQKPLQISSAYSRMLVHKLRAESDA
                     IMIGSRTALLDNPRLTLRHWPGRHPLRIVMDRQNTLSKDSLLLNDELPSLIFTENFSD
                     RRGNKEWLALTGHNSGSMLEDVLAVLHARDVHSLLVEGGSTLQKAFLDAGLWDEIQTE
                     TWHHTIKNGHRALRIPEDQLKACSITSTSIPSENRTITHYLRKH"
     gene            6668..7519
                     /gene="prmC"
                     /locus_tag="PLI37_01725"
     CDS             6668..7519
                     /gene="prmC"
                     /locus_tag="PLI37_01725"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOQ57771.1"
                     /translation="MQTFFSYAAQSLKEQYRPEEIRRLSYALLEDCCGFSKNDIYAGK
                     DTEISTKQRRQLHEKLGRLAEGEPLQYITGVAYFYDLKLSVNPAVLIPRPETEELVEW
                     IINDNKGKTGRILDVGTGSACISLALARHLPGMQVEAWDISPGALALARKNAADLGLD
                     VAFIEQDLFRFNPGDRASVYDVIVSNPPYVTTAEALKMEKRVLEHEPALAMFVPDNDA
                     LIFYRALAALAARTLKPGGSLYVEISIYYGREIVQLFEEAGFEETVLRQDISGRDRMI
                     KAAKYHG"
     gene            7512..7973
                     /locus_tag="PLI37_01730"
     CDS             7512..7973
                     /locus_tag="PLI37_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOQ57772.1"
                     /translation="MDNYSQALNWAQHYCSKAERCSGDVLIKLGKYKLDEASTKRLLE
                     NLRKENYLNDLRYARAYTMDQFRFGRWGRIKIRYMLRRKGLAEADIQQALEQIPGDEY
                     LDLLKALINEKQKHYAGLEAYEKKAKLFRFVYSKGFEPDLINQCLDLSTDY"
     gene            7946..8632
                     /locus_tag="PLI37_01735"
     CDS             7946..8632
                     /locus_tag="PLI37_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOQ57773.1"
                     /translation="MPGFINGLLNLLYPPLCVVCGRNLIAGEEHLCSACLYALPRTRF
                     HSERQNPVCERFWGKLPFEKATAAFHYQKESHMQLLMESFKYKSNRQLAFYLGSYAGM
                     QLGKQDFFEQIDLILPVPLHKNKERRRGYNQSELIARGLAHCSGLPVDTGSLGRVVEN
                     PTQTRRSIWERSRNVQQIFRLNSSEKLAGKHVLLVDDVLTSGSTMEACGNALLEARGL
                     RLSFFALALA"
     gene            8744..10036
                     /locus_tag="PLI37_01740"
     CDS             8744..10036
                     /locus_tag="PLI37_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOQ57774.1"
                     /translation="MKKKTILTTFLIFGLLVHSRAEDIVKSGFNLGALPAISYNSDLG
                     FQYGVILNLFHYGDGSRYPAFDHSMYLEVSRYTKGTGVYRLYYDSDRLIKGVRSFIDL
                     SYMTNLMQDFFGYNGFRSVYSVDSVAANSAFYKMHQNKFHLMADFQGNLPVDNLKWLL
                     GYGLYNYSIAPVNLEKLNKGKSPEEQIPDRPGLYQKYVEWGLISPGEKHGGMLNLLKA
                     GLMYDSRNAKTNPDKGIFTEALLEMAAPLLNESPYVNYIVMHRQYQSLIKNRLNAAIR
                     IGVQGKIGNKAAPFYRKPQLISTFATRTVTTGLGGNSTLRGILQNRVVGDAFALANVE
                     LRWKMINFRWFNQNFYIGTNYFWDAGYIIDPIDWDETVLTANPAYFADKDGLHHSAGL
                     GLKIAMNENFVLSADFGKAFNKQDGAGLGTYIGLNYLF"
     gene            10054..10695
                     /gene="pyrE"
                     /locus_tag="PLI37_01745"
     CDS             10054..10695
                     /gene="pyrE"
                     /locus_tag="PLI37_01745"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOQ57775.1"
                     /translation="MKTLKKLIAEKLLKIKAVKLQPSNPFTWAMGWKSPIYCDNRKTL
                     SYPQLRNFIKIELARIIREKYEQVDAIAGVATGAIAQGAMVAEELCLPFVYVRSSPKD
                     HGLENLIEGDLKPGMKVVVIEDLISTGGSSLKAVEAIRKDGSEVIGMLAIFTYGFDVA
                     IERFKQAKVELTTLCDYDTILETALETNYISDADVKTLQIWRKDPANWKPRIQ"
     gene            10704..11120
                     /locus_tag="PLI37_01750"
     CDS             10704..11120
                     /locus_tag="PLI37_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOQ57776.1"
                     /translation="MTEFKTQTGRIAAPACRVFEVLSDWNQLEKIKEKIPESKLRGLE
                     FDADSCRFVIDSYGVGEIALCISERNPCSVIKIKAEQSPIDFNAIISIEEENPEVCLL
                     TIGLEAALPFMLKHILSPSIQTGLDKILNLLTEYSY"
     gene            11126..>12399
                     /gene="argH"
                     /locus_tag="PLI37_01755"
     CDS             11126..>12399
                     /gene="argH"
                     /locus_tag="PLI37_01755"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOQ57777.1"
                     /translation="MKLWQKNTEVDKDIERFTTGKDREMDLYLAEFDVLGSAAHARML
                     EQIGLISPEELPLLLRGLREIYEQIQAGKFTIEEGVEDVHSQVELILTRKLGDLGKKI
                     HSGRSRNDQVLLDLKLFTRHKLEEVLEAVHALFELLIKLSERYKKVMMPGYTHLQIAM
                     PSSFGLWFAAYAESLVDDVQVLQAAFKICNRNPLGSAAGYGSSFPLNRSLTTQLLGFE
                     GLNYNVVYAQMGRGKMEKTTACALASVAGTIAKLAYDACLFSSQNFGFIALPAEFTTG
                     SSIMPHKKNPDVFELTRAKCNRLQQLPENISAITQNLPSGYFRDYQLLKELFLPAFDE
                     LIDCIKMTSASLERVRINEHILDDPRYEYIFSVEEVNKRVLNGVPFREAYRQVGMEIE
                     AGRFRADKNIKHSHEGSLGNLCNDKITARMNE"
     assembly_gap    12400..12499
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12500..>14949
                     /gene="clpB"
                     /locus_tag="PLI37_01760"
     CDS             <12500..>14949
                     /gene="clpB"
                     /locus_tag="PLI37_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOQ57778.1"
                     /translation="FNKCGVQPQTVVQAVERQMSSYPKVSGGENYLSKEATAVLQKAT
                     AYAAELKDQFVSLEHILLALLSEKSPVSNILKDAGLRLSELKLAIRELRKGKSADTEN
                     AEEVYDALNKYAVNLNEMARNGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGTG
                     KTAIAEGLAHRIVRGDVPENLKTKQVYSLDMGALVAGAKYKGEFEERLKAVVGEVIEA
                     EGEIVLFIDEIHTLVGAGKGEGAMDAANILKPALARGELRAVGATTLNEYQQFFEKDK
                     ALERRFQPVMVNEPDIADSISILRGLKERYENHHKVRIKDEAVLAAVELSSRYISDRF
                     LPDKAIDLMDEAAARLRLQADSVPEELDEISRRIKQLEIEREAIKREKDELKLNQLGK
                     ELADLVEKETAMKAAWQAEKSHIDRIQELKAEIESLRFEAERAEREGDYGKVAEIRYG
                     SIQQAEAAIKKESDALKTLQQSGQLVKEEVDAEDIAEVVSRWTGIPVYKMMQSERMKL
                     LRLEEELHRRVIGQDEAINAVADAIRRSRAGLQDPKRPIGSFIFLGSTGVGKTELAKA
                     LAEYLFNDENTMIRIDMSEYQEKFSVTRLIGSPPGYVGYDQGGQLTEAVRHKPYSVVL
                     FDEIEKAHPDVFNILLQVLDDGHLTDNKGRKVNFKNTIVIMTSNLGSELIRERMDQMN
                     ESNRDQLMEQTREEVLELLKRTLRPEFLNRIDEIIMFKSLTRSEIKEIVRLQLEQLKS
                     NLAERGLRMRYTDKLLDHLAKEAYHPQFGARPVKRLIQRELLNSLSKALLSGSIQEDS
                     LVEIDESGGEILFRSIINP"
     assembly_gap    14950..15049
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15104..15964
                     /locus_tag="PLI37_01765"
     CDS             15104..15964
                     /locus_tag="PLI37_01765"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HOQ57779.1"
                     /translation="MLIKRFVLSLMLLNLSGLLLAQTDVSLFWRKDSLQQTEIFSAEG
                     DLYRLVGHHGPAIENKFMALRLYFNHSGSIDVYSKKIHRLELAQALWYPSEKMQQEQG
                     FGCDEYKVGSTVGLGGINLWDGRQVVKLTATRGRTARVSKNEKGASMEMLAKGIAYKD
                     KMVDILIRVSVNNTDRQATVEAVCTSGHKVQFLTGVNRHPGNSVFNRDGRIGVWGRHP
                     ADVSKAPVLIGGGMKYRPSDFAIKRVTDDAIQLISKPAKKLKTTIVSAGEREEELNTE
                     QAFFDYVKSF"
     gene            complement(16086..16340)
                     /locus_tag="PLI37_01770"
     CDS             complement(16086..16340)
                     /locus_tag="PLI37_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOQ57780.1"
                     /translation="MKKDLHPTNYRPVVFKDMSNGDTFISRSTIQAKETIEIDGETLP
                     LVKLEISSTSHPFYTGKSKLVDTAGRVDKFLNRYGNRNKK"
     gene            16542..>16822
                     /locus_tag="PLI37_01775"
     CDS             16542..>16822
                     /locus_tag="PLI37_01775"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOQ57781.1"
                     /translation="MISYKELGLVNSREMFKKAIAGQYAIAAFNFNNMEQLQAIIQAA
                     VETRSPVILQVSSGARKYANQTLLRYMAQGAVEYAKELGLEHPQIVLHL"
     assembly_gap    16823..16922
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16923..17692
                     /locus_tag="PLI37_01780"
     CDS             <16923..17692
                     /locus_tag="PLI37_01780"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOQ57782.1"
                     /translation="EYAKELGLEHPQIVLHLDHGDTFELCKSCIDMGFSSVMIDGSHH
                     PYEDNVALTRKVVDYAHKYDVTVEGELGVLAGVEDEVSSAVSHYTRPDEVVDFVTRTG
                     VDSLAISIGTSHGANKFTPEQCTRDANGVLIPPPLRFDILEEIEKRIPGFPIVLHGSS
                     SVPQDLVAIINKYGGAMKDSIGIPEEQLRRAAKSAVCKINIDSDGRIAMTAAVRQVLA
                     TKPAEFDPRKYLGPARDSLKELYKHKLINVLGSANKL"
     gene            complement(17797..18789)
                     /locus_tag="PLI37_01785"
     CDS             complement(17797..18789)
                     /locus_tag="PLI37_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ57783.1"
                     /translation="MQYTPSTDRYVNSVGYNYCGKSGLQLPRISLGLWHNFGDVNDQD
                     EAKKMLFTAFDNGITHFDLANNYGTPFGSAETNFGKMLKNDFKSYRDELIISTKAGHL
                     MWDGPYGDWGSRKYMIASLNQSLKRMGLDYVDIFYSHRYDPNTPLEETMGALSDMVKQ
                     GKALYVGISKYPPDKALEAIRLLSENGTPCLIYQGRYSLFNPEVEQSIFPLVEEKNIG
                     FIGFSPLAQGMLSNKYLEGIPPNSRAGQEFGFLKKEQITPEKIEKIKQLNDFARKRQQ
                     TLAQMALAWCLRDKRVTSVIIGASSVKQLSENIQALDNLHFTEEKLRLIGSSVY"
     gene            complement(18825..>19288)
                     /locus_tag="PLI37_01790"
     CDS             complement(18825..>19288)
                     /locus_tag="PLI37_01790"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOQ57784.1"
                     /translation="MLTELNRMEAHYTELFTGSRRQSIRTEVLEYTPEKIPSYEILFR
                     FSSQKGIVDKEDLSGYPVHFLVHNPGTDAISQQKDSISGKKGETSQQTCALYYYHPNR
                     IDISLLKGQTVLFKDNFVIAQFGDLMQLPASEQLMLELDPETGAIVRIKNR"
     assembly_gap    19289..19388
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19389..20110)
                     /locus_tag="PLI37_01795"
     CDS             complement(<19389..20110)
                     /locus_tag="PLI37_01795"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOQ57785.1"
                     /translation="MKKLLFLSLVVLGLSGNLTAQIQSRIVEGPIPYEAFSYHLPKTV
                     FKVRVTIRTTHEEPGIYARYSERLLGISDIVTTERVNNELVSVELDTYTLPDPKRVFY
                     IQAAAPQVSKRRASDTQVPELNLQFTSEGILRAVNMDLSLTESCSGKASKQETTISCP
                     ENANDNSASSAASIHTHDMQLSGSSIRMAELAAKKVFELRDTRLSLLQGELSTMPQDG
                     ASLEKMLTELNRMEAHYTELFTG"
     gene            complement(20123..21655)
                     /locus_tag="PLI37_01800"
     CDS             complement(20123..21655)
                     /locus_tag="PLI37_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOQ57786.1"
                     /translation="MALNIYTTAQIRQLDADTIQIEGIDSSELMERAALGLFERIKTL
                     IAPNKSILALAGPGNNGGDALALSRLMLEAGFQLRCLLLSGNKTLSHDCALNAKKLIE
                     LFPSSFVFDATPEDLVKEAPTDYLIDGLFGSGLNKALEGDFARIVHWLNKQKALRISI
                     DLPSGLYGEDNRTNKPENIVRADLVLGIQFPRLAFLLPENEDYIGRWELTPLDFHPEA
                     LARQTTNYSLVEQNDIRAVLRNRKKFSHKGNYGHGLLIAGSAGMPGAAILAGRAALRA
                     GLGLLSIRMPSALSACIQSAVPEAMTSIDPLSENNISSMENLDLDRFSAIAIGPGLGT
                     GQGQTRVMKALLKETRKPLVIDADGLNILAAEPSLLSLLPRGSILTPHPGEFDRLAGA
                     SDCGYERLEKASAFAKKHGVYLILKGHYTCSIFPDGSRHFNNTGNSGMATAGSGDILC
                     GILLSLLVQAYPPAQCCLLGVYLHGLSGDLALKEQSEESLVAGDLIQYLGKAFKHSKG
                     QI"
     gene            21792..22331
                     /gene="hpt"
                     /locus_tag="PLI37_01805"
     CDS             21792..22331
                     /gene="hpt"
                     /locus_tag="PLI37_01805"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOQ57787.1"
                     /translation="MKTVQVKDRSFALLLTAERIRKRVSELALEINKDMEGKNPLFLV
                     MLNGAFMFAADLLKEINVPCEISFIKYDSYRGTKSSGRLNKLIGLKESLRNRCVIVLE
                     DVVDSGLTMSRLFEELKTKKPAELYLATCIFKPEALKSEVKPDYVGFNLPDVFLVGYG
                     LDYEGYGRNLEAIYELKSR"
     gene            22376..22963
                     /locus_tag="PLI37_01810"
     CDS             22376..22963
                     /locus_tag="PLI37_01810"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOQ57788.1"
                     /translation="MLNIVIFGGPGSGKGTQSALIAEKYNLIHLSTGDLLRDEVASGS
                     ELGKLIHSFIDKGNLVPDQLMLDILSRTIDNLNGKKGVIFDGFPRTIAQAEALDEILE
                     KRNEKLNLLLHLKVADEILQERMLFRSKTSGRSDDTPETIKKRIDIYHQVTAPVINYY
                     RHRGLYHAIKGNGSGTVETIFAEVSSYISAYQQKR"
     gene            22963..23949
                     /gene="obgE"
                     /locus_tag="PLI37_01815"
                     /pseudo
     CDS             22963..23949
                     /gene="obgE"
                     /locus_tag="PLI37_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
     gene            24137..24919
                     /locus_tag="PLI37_01820"
     CDS             24137..24919
                     /locus_tag="PLI37_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57789.1"
                     /translation="MSLFISYKQKRGRFLSALLCSCLVFLPVKAQSSGAYLSEANNLL
                     LNTGFEIWENNLPAHWFGSASNIPETSVFQCAEAHSGESSCRLVRTQKTHVRFSSAPL
                     FLNLGNYRLSYYVKGSGCIRNSFYNGSSYAAYSDYDTLSNQQEWMKKVYEFELKKDVE
                     VQLLFSLCLTDSIGLFIDDVLFESIPAGLNRPVEFSPLIRTAQGLLHLQLSNPQALVV
                     YDMLGRKVCERNVQGDLSIPLSRGYYLIALSGHPKPVKVYIP"
     gene            complement(25028..25426)
                     /gene="ssrA"
                     /locus_tag="PLI37_01825"
     tmRNA           complement(25028..25426)
                     /gene="ssrA"
                     /locus_tag="PLI37_01825"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(25432..26961)
                     /gene="rny"
                     /locus_tag="PLI37_01830"
     CDS             complement(25432..26961)
                     /gene="rny"
                     /locus_tag="PLI37_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOQ57790.1"
                     /translation="MYIVYLVLALSVGIFLGWLIFRYILNGKYLYKIKKAELEAEAIR
                     KNKMQEIKDKFFAMKAELEKQITQRSNRVQSYENKVKQREMQLNQKQDELQRKKNELE
                     IIRENLEAQLEIIDKKKQELDKQHRKGVERLEALSGLSAEEAKERLVESLKEEAKTDA
                     ASYINEIMDEAKMTANKEAKRIVVQSIQRVATETAIENAISVFHIDSDEIKGRIIGRE
                     GRNIRALEAATGVEIIVDDTPEAIVLSAFDPVRREIARLALHQLVSDGRIHPARIEEV
                     VAKVRKQIEEEIVETGKRTTIDLGVHGLHPELVRMIGRMKYRSSYGQNLLQHARETAN
                     LCAVMASELGLNPKKARRAGLLHDIGKVPEDEPELPHALLGMKLAEKYKEKPDICNAI
                     GAHHDEIEMQTLLAPIVQVCDAISGARPGARREIVEAYIKRLNDLEQLALSYPGVIKT
                     YAIQAGRELRVIVGAEKVDDKNTEQLSAEIAKRIQDEMTYPGQVKITVIRETRAVSYA
                     K"
     gene            complement(27062..27358)
                     /locus_tag="PLI37_01835"
     CDS             complement(27062..27358)
                     /locus_tag="PLI37_01835"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOQ57791.1"
                     /translation="MKDDTFRIELYLAEKYYPLRIKRSKEEAYRKAAKLVNDAVTEYR
                     NKFGIRGSDLDIKDILAMVACHFAFEKLEFEKSSDQNILMESISKLDAELGEII"
     gene            complement(27361..27657)
                     /locus_tag="PLI37_01840"
     CDS             complement(27361..27657)
                     /locus_tag="PLI37_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57792.1"
                     /translation="MTEEQQSLLIQFEARVRQAIALCKTLREENKALKEALLQKELSF
                     LNIQEELRDISQKYDRLKLAKMMSANDDDLKATKQRLSKLVREINTCIALVNES"
     gene            complement(27849..>30138)
                     /locus_tag="PLI37_01845"
     CDS             complement(27849..>30138)
                     /locus_tag="PLI37_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57793.1"
                     /translation="DPVIDHNRNDLFLSDFPGLVLVPGELLSYKSPLYTAGTEGTYVG
                     ALSSYYVPAFIEQPTGAIVKNLAELTEALAYTDYESADITIELMNCTDEGGVYLLGNS
                     GFAFPQTGGDLTIKNAEGQNPVLAGRLTSNNGVKAATLLYEGLEFNAQSVYDAYNTDT
                     YSPLYMTGIDSVMVGFNVRNCLFTNLPFKQLILSANKIANYAVIKDFVMEHCVIKDVG
                     VNQDTMAQHSGHMFQLSNKKTYEFDHFTFRNNIVNNWRNSQVFNINRDGTTADSTDVN
                     FVVTIENNLFYKFGGNATNVRNFLEYTKSLGGLTGVININNNIFYERGSQAGFPHAKL
                     TMFLPAPGQSVTLNVLNNFFYPDNVVKDESTPENYPNLRLVGPAETDITFSRNDLFMD
                     SFEGLSKVFRNEARLTISKQSPLFEAGTGGSFIGPEQIYVGNAGTPVIAFLTKQKTMA
                     ATAATVENDPVYLMMAADDNFIVDQIVLTDVSATSPVDLDAYDIALVQESFGGGDKIL
                     TPAGPLGLAKFDLPVLYNKTYAFRGGRALASGQPGSGAEQQGLSITVDPAQQGNDLFK
                     GLTFADDNTLTLFKAPAMDDGTLGAEKSIKALNYAKEVVLSGENTPIAIPTGVTDAVI
                     FINDLPAGTVVGGETLAERMITVGFNFGAMCADNGTNMTNAAFTIWRNALYMLAGMEV
                     PSDPYDALPQTDGVQKNVVFTMGGNLYVQATEQTNINIYNVMGALVRTVRLEQGLNTI
                     EGLSQGQIYVVKIDSEAIKVVL"
BASE COUNT         7773 a         6585 c         7075 g         8205 t
ORIGIN      
        1 cgtaagcggg aatacgcgaa aaaatgtatt ccatatcgac taaggcgtat ttttgagcaa
       61 atacggccat gctgctaaaa agcaaactga ttattaataa agaagctttt ttcataccgt
      121 tgagattttt gtatttgact taaaagccgg tcaaagttaa gaacaaattt taaaattctt
      181 gtcctatgac gaaatgaaat tcaccgccat gggctttggt ttccgtgccg acaggatcaa
      241 atccgtaagc ccagtcaatt cccaataatc ctactatcgg gatgaatatg cgtacgccta
      301 cgcctgccga acgtttcagt tcaaagggag aataatgctt aaaatcagac cagaggttgc
      361 ctgcttccaa aaacgcagaa gcgtaaatgg tagccgattg ttccatcaac aagggatagc
      421 gcagttccaa accggccctt tgatagagcc agccaacgcc cagatcgccg ttgccgtaac
      481 cccggagcgc gatgatatcg taggcatagc tgctggagta gcctgtcata ccgtctcctc
      541 ccatatagaa cgtgccgaaa ggcgatcttt tgtatttgtt aaaggcgccg agcaaaccaa
      601 tatcaaaacg ggtggccata actaatttcc ggtttccgtt caatggggta taggttctgg
      661 aacggaaacg aactttgtag tattctatcc agtggtagag ttcctgactg ctcatttttt
      721 tgtaatcttc gtcggttttg ttgtcgagca aagaaaacgg aggagtgatc cccagcgata
      781 aggaatagga agaacccgag cgggtgtaca aaggattgtc cgtggagttt ctgctcaggg
      841 taaagttaag gctcaggtcg ttgctccggc cgtttgaaat aaggaaaaac atccaatttt
      901 ccaggttgta gtgctgataa gaaaactcag agaacaggtg aaaataatcg tccggccagg
      961 ttaacctggt acccaaaccg gcagacaacc cccacatttt tatgtatttg gtggggtcca
     1021 tttcggtcag gtaataatcg ccgtaatcgt aagtattgcc gtaagtggca taatgcatca
     1081 tgtaattgct gtagtaattt tcgtaatacc tgttgcttac gcctgtctga taagaataat
     1141 aacccgagaa agaaaaagag ttgggttttt tcccgcccag ccagggttcc ataaacgaaa
     1201 aactgtacga ttggtagtat tgcccattgg tgcggccggt aatactgaag gtttgtccgt
     1261 ctccttgagg caaaactcct tcacccagcc ttagcaaatt tcgcatggaa aagttggtaa
     1321 atttcagacc gatcgatccc actatacctg tgcttcccca gccgagagaa aattccacct
     1381 ggtcgttcga tttggaggta aggttcaaat cgatgtctac cgtaccgtct tcgttgggaa
     1441 tggggttgat gcccatggtt tccggatcga aatgccctgt ttgggcgatt tcacgggctg
     1501 agcgcattag ggcggctttg ttgaatagct ctccgggttt ggtgtagagt tctcgccgga
     1561 tgacgtgttc atagagccgg tcgttgccgt ttatgtttat ccgctttatg ctggccggct
     1621 ggccttcgct gagcctgatt tccaaatcaa tggagtcttc agccacagac acctccacag
     1681 gtatggcctg aaaaaagagg tagcctctgt ccatataaag gtcggttatg gcgtcttcgt
     1741 tgggcgattg atgtaagcgt tcgtttaatc ttttttgatt gtagacatcg cctttttcta
     1801 tctggagcct catgttcagc tcttcggaag aatataaagt gttgccaacc cagcttatat
     1861 cgcggataaa gtattgacgt ccttcgtcga ctttcaggta gacgttcacc gtattgtcgt
     1921 cgaagcggta aacggtatct tctacaatga cggcatcgcg gtatccgtat tcnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttttgta gcctttgccg tcgtagtgtt
     2101 ttttgatcag tatttccgct ctgtcgatga tattgggggt aatctgattg cccacaatca
     2161 tattcagtcg gggttcgagt tcctcaatct cggatttctt catcccgttg taattgattt
     2221 tcgaaattcg gggccggggg ctgagaacaa tgcggagcca tatttcgttg ccttctattt
     2281 tgtctgctat tattttgaca tccgaaaaaa ggccgtgcct ccagaagcgc ttcacggctt
     2341 cgctggtttc gcttccgggg actcgtatta tctggccgac ggacaatccg gataggccaa
     2401 tcagcacata atcatcgtaa ttatctatac cttcaccttc gacggcaatg tcggctattt
     2461 ttaattctct gggataggaa taatatatct ccggcaaggc ctcttcttgt gcaataagcg
     2521 gaggtaaggc gcaaaaaaca cataaaaagc cggcaagtag cctgtatatt attgagtgag
     2581 tcttgttcaa tgtcctattt tgttagttgt tcacttgttt tcccaaagcg tctttctctg
     2641 gactgatatt cgatgatggc ttcataaaaa gcttctttcc tgaagtcagg ccaggccgtt
     2701 tcggtaaaat acaattcggc ataagcagct tgccacagca taaaattgct gatacgaatt
     2761 tcgcctccgg tgcggattaa taaatccggg tcgggaatgc cggctgtgca aagatgtttc
     2821 gaaaaacaat cttcatccag ctcctgttcg agaattcttc cctcggatag gcctttggcg
     2881 atgcttttga ccgcttccat aatttcccag cgggaggaat aactcaaagc gagtactaaa
     2941 ttcagttcat taccctgagc cgtattatgc ataaattgct gaactttatc ctgaatgtct
     3001 ttttctaatc tgtctaagtc tcctaccacc aatccccgta cgccattttt gattaaatcg
     3061 tcggtttcgc ggtcgatggt ttccgtcata agccgcatca atgcgttgac ttctttttga
     3121 ggacgattcc agttttccgt cgaaaaaacg tataaggtta aataagaaag tcccaactca
     3181 cgagccgctt ccacaacggc acgaacggaa ttaatccctt cggtatggcc gaaaatgcgg
     3241 tctttgccac ggcctttggc ccaacgtccg ttgccgtcca ttacgatggc cacatgtttg
     3301 ggcagagttg atttgtctat tttttctaat ccggacatct tatttataac atttcgatcc
     3361 tatataaaac aggtcataac ttaggtaaaa ggccaggaca aaatagccgt ccttattttt
     3421 gttccatgca ctttgagcaa tccagggatc atccaaatga aacaaatttg attgaggcgt
     3481 gtaatccagc ttatcggaaa agctcatgcg ataagtgcat tccagcccca gattgaaacg
     3541 ggcaggtaat ttcagcttaa tgcccaatcc caaaggaata ttggcggtaa gggcgttttt
     3601 atcggtttta tatccggtta gtcctattcc gcacaatacg taaggggaaa cgttgccggc
     3661 accgggatag tagtctgctg ccccgaattc ataaaaattg aattcaaatt gcaggcctcc
     3721 atcaagaact cttcgggaaa aatccaaggc ttcggctccc ggatattgct ccagcttgtc
     3781 cgtggttgtg cccgatatcc ccgcactcag gagattccct tttatggaaa aacgccggtt
     3841 caggtgatag cgcattaata ccccgtaagc aggatgcact ttctcgaaaa gcttagtctg
     3901 gttggcatcg cccgtgtaaa acgaaaggcc tcccagaccg ccgatttcta tgcgataact
     3961 ctgtccgtac attgctgctg aacagagtaa taaacaaatc gaaaagaatg ctcttcgtcc
     4021 tgtcatgctg attagttgct gttacatctc ctattctaac gcaaaaaagc cgaattcatt
     4081 ttgcagatac gcttatttcc cgggaaataa ttttctgtga atgttgcctt tccacacttt
     4141 atttgagatg ttgctgccgg attctccgcc gaaaatccat atatacttgc cttcttctac
     4201 aaaaatatcc tggtaagcca atcctccgtc tatgctgtaa aattgaagca gggtatcggc
     4261 tgccgaccag gtcacgccgt tattcccgga aacatacaaa gtattgtcgc aaagcccccg
     4321 gctgtcgagt cccccgtaat agaacatttt atcgtcgtaa tgaaataagg aagcaccttg
     4381 tctgggttcg atatgcaggg gtttagtgtt gactttaatc caatacaggc catccatggt
     4441 actccagcta tctccattca gggcacctga ggcgttttta ccccccagca ggctgatata
     4501 gggaacatcg gttactgttt tgtatttggt aagggcatac tcgcagaggg ggaaattttc
     4561 gggaacggca gtgccgcatt cccaaccctc agactctccc tcgaagcggg caaagcgtaa
     4621 atctccgctg ttgtctgcta cgattccaac caaggcatct tcgctgagaa acttgcgtcc
     4681 ttccaattta gcaaagaggc ttacaacttt ggtgctgtct gcagatccgg accattgatt
     4741 cagatcagcg gaataataca gcctcccttg cttcccaagg aggtaatatt gtcccttgaa
     4801 ttcagtgagg ctctgaatga aaatagtatc gtccagcccg gatacagggg catctgccga
     4861 ccaggtaagg ccttgatcgg tcgaagtata gctgatcaca gagcctgtat cgggagcaaa
     4921 aaaggctttg aacaggccat tgtatgaaat ggttttattt cgggcggcaa tggtcgggaa
     4981 ccggctgctc aggacattca ttaccatgga atcgggattg atctgatgag catttaccct
     5041 aatccggtat ttcttttggt gtttgccgtc gtcggatgta tttgtcaggg ataagggacg
     5101 gctgaaatcc aatacgtcgc cgggcttcca gagcatagtg tcgttgataa atacattacc
     5161 gttggaggaa aaaaagacga tgctgggaac cagcgaatca atccgtgttt gatatggcag
     5221 cgaatcatta ttgtaaatga gcccggcaaa attgtcgacg taaaaaacgt agcgggtaat
     5281 tcccggcacc gaatcaaacc cgaaatgttg cacattagcc agggaaaacg attgatcctc
     5341 aaaactatgg caagaggaaa cccccagcag tacagggctg agcaatacca gcaagcagac
     5401 ttttttcccg atactatata gtagtttgtt cattcagctc caaaaattaa gcgcaaaagt
     5461 aagaagaatt tcagctttaa atacaaataa ctgttttttt atgaataatt agtctgataa
     5521 cagcctttct gctcagtgtt tgcggagata atgcgttatg gtcctgtttt cggatggaat
     5581 acttgtgctt gtaatgctac aggctttcaa ctgatcttcg ggaatgcgca gggccctatg
     5641 tccgtttttt atggtatgat gccaggtttc tgtctgaatt tcatcccaaa gaccggcatc
     5701 caaaaaggct ttttgcaggg tgctgcctcc ctcaaccaac aggctgtgaa catcgcgtgc
     5761 gtgcagtaca gccagcacat cttcgagcat actgccggag ttgtgtccgg tcagagccag
     5821 ccattctttg ttgccgcggc gatccgaaaa attttccgta aaaatcaggc tgggaagttc
     5881 atcgttcagc aacagcgagt ctttggacag ggtgttttgg cgatccatta ctatccgcag
     5941 gggatgtcgt ccgggccagt gcctcagcgt cagacgggga ttgtccagca atgccgtacg
     6001 agagccgatc ataatggcgt cggattctgc cctgagttta tgaaccagca tccggctgta
     6061 ggcgcttgat atttgtaagg gtttttgccc gtctcccggt tgacgaatgt aatctgaata
     6121 agcatcggcc gtctgagccc atttgaggac aatataggga cgttgctttt gataatagca
     6181 gaaaaaactt ttgttcaggt agttggcggc ctctgcctcc agacccagtt ccacttcgat
     6241 gccggcggct tttaatttgg ctatgccctt tccgcaaact ccggggaaag gatccggaca
     6301 ggcaatgact actcggggaa tttttttttc gatgatcaga tcggtgcagg gcggagtttt
     6361 tccgaaatgc gagcagggtt caaggttgac gtagagtgtt gattccttga gcaactcccg
     6421 gttttttacc gactttatgg cctggggttc tgcatgtgcc ttgccggcac tgtgatgcca
     6481 gccttcgccg atgatggtgt tttgatgcac taccaaggct ccgaccatgg gattgggaga
     6541 acaactgatg cgtccgttgg ctgccagttg caggcaacgt tgcatgtaga gagcctcagg
     6601 atttttgtcg gcacttgtgt tgtggagcaa ggagaaatgc attatttttg taaacaaatt
     6661 gttaaccatg caaacatttt tcagctatgc ggctcaaagc ctgaaagagc aataccggcc
     6721 cgaagaaatc aggcggttgt cgtatgcttt gctggaggat tgctgtggct tctcgaaaaa
     6781 tgatatttat gccggcaaag atactgaaat ttcaactaaa caaaggcggc agttgcacga
     6841 aaaactggga cgtctggcag aaggcgagcc tttgcagtat ataacgggag ttgcttattt
     6901 ctacgatctt aaactcagtg tcaaccctgc ggtattgatt cctcggcccg aaaccgagga
     6961 gctggtggaa tggataatca acgataacaa gggcaaaacg ggtaggattt tggacgtggg
     7021 taccggcagt gcctgcatat cgctggcatt ggccagacac ctgcccggta tgcaggttga
     7081 agcctgggat atatcgcccg gtgctctggc tttggcccgc aaaaacgctg cagatctcgg
     7141 gctggatgtg gcgtttattg aacaggattt gttccgtttt aatcccggag accgggcttc
     7201 ggtgtatgat gttattgtga gcaatcctcc ctatgtgacc acggccgaag ccctcaaaat
     7261 ggaaaaaagg gtgctcgaac atgaaccggc tttggctatg tttgtgcccg acaacgatgc
     7321 cctgatcttt taccgggcgc tggcagccct ggcagcccga actctgaaac ccgggggaag
     7381 tttgtatgtg gaaatcagca tttattacgg cagggagatt gtgcagctat ttgaagaggc
     7441 cggtttcgaa gagacagttc tccggcagga tatttccggc cgtgaccgaa tgataaaagc
     7501 cgctaaatac catggataat tattcgcagg cgcttaattg ggcgcagcat tattgctcca
     7561 aagcggagcg ttgttccgga gatgtcttga tcaagctggg aaaatacaag ctggacgagg
     7621 cttcaaccaa acgcttgctc gaaaacctgc gcaaggagaa ttacctaaac gatctgagat
     7681 acgccagggc ttataccatg gatcagttcc gcttcggccg atggggaagg attaaaatcc
     7741 gctatatgct gaggagaaaa ggccttgccg aagccgacat tcagcaagcc ttggagcaaa
     7801 tacccggcga tgaatatctc gacttgctga aagctttgat caacgaaaaa caaaaacatt
     7861 atgcaggttt ggaggcttac gaaaaaaaag ccaagctctt taggtttgtc tacagtaaag
     7921 gctttgaacc cgatcttata aaccaatgcc tggatttatc aacggattat taaatctgct
     7981 ttatccccct ctctgcgttg tgtgtggcag aaaccttatt gccggcgaag aacatctttg
     8041 ttcggcttgc ctttatgctt tgccgcgcac acgtttccat tccgagcgtc aaaatcctgt
     8101 ttgtgaacgt ttttggggaa aattgccttt cgaaaaggct acggcagcct ttcattatca
     8161 aaaggaatcg catatgcagt tgctgatgga gagttttaag tataaatcca accggcaact
     8221 ggctttctat ctcggaagtt acgccggaat gcaactgggc aagcaagact ttttcgagca
     8281 gatagacctg attctgccgg tgcccttaca taaaaacaaa gagcgacgga ggggttataa
     8341 ccaaagtgaa cttatcgcaa ggggcctggc tcactgcagc ggcttgcccg ttgacaccgg
     8401 ttctcttggt cgtgtcgtgg aaaatcctac ccaaacaagg cgcagcattt gggaacgaag
     8461 ccggaatgtg cagcaaattt tcagattaaa cagttccgaa aaacttgccg gtaaacatgt
     8521 tttgctggtg gacgatgttt taaccagcgg ctccaccatg gaggcttgcg ggaatgcttt
     8581 gctggaagcc cgtgggctta gactgagttt ttttgccctg gctttggctt aattctttga
     8641 gacactgtct ttgtatattg caaaatatct gtacttttgt ccctgattta aaacagcttg
     8701 agaagatctg agcaaaaagg tcaactccct aattatgacc gctatgaaaa agaaaacgat
     8761 cctgacgacc tttttgattt ttggtttgct tgtccattct cgtgcagaag acattgttaa
     8821 atcgggattc aacctgggag cattgccggc catatcttac aattcagatt taggttttca
     8881 atacggagtt attctgaatt tgtttcacta cggggatggc tcgcgttatc ctgcttttga
     8941 tcattccatg tatctggaag tgtcgcgata taccaaaggt acaggcgtat accggcttta
     9001 ctacgactcg gaccgtttga taaaaggagt gcgaagtttt atcgatttgt cgtatatgac
     9061 caatctgatg caggatttct tcggctataa cgggtttcgt tcggtttaca gtgtcgattc
     9121 ggtggcagcc aattcggcct tttacaagat gcaccaaaat aaatttcatt taatggccga
     9181 ttttcaggga aatctcccgg tggacaattt aaagtggttg ctgggttacg gcttgtataa
     9241 ctacagcata gctccggtca accttgagaa actcaacaag ggtaaatccc ccgaagagca
     9301 gattcccgac aggcccggct tgtatcagaa atacgtggag tgggggctga tcagtcccgg
     9361 tgagaagcac ggcggaatgc ttaacctgtt gaaggccggc cttatgtacg atagtcgtaa
     9421 tgccaaaacc aatccggata aaggcatctt tacggaagcg ctgctcgaaa tggcagctcc
     9481 tttgctgaac gaaagcccct atgtcaatta cattgtgatg caccgtcaat atcagtcgct
     9541 gatcaaaaat cggctgaatg cagctatacg aattggtgtg cagggcaaga tcggtaataa
     9601 ggcagctcct ttttatcgta aacctcaatt gatttccact tttgctaccc ggaccgttac
     9661 caccggttta ggcggaaact caactctgcg cggcattttg caaaacaggg tggtgggcga
     9721 tgcctttgcg ctggccaacg tggaattacg ctggaaaatg attaattttc gctggtttaa
     9781 ccaaaacttt tacatcggga ccaattactt ttgggatgcc ggttatatca ttgatcccat
     9841 cgattgggat gaaacggttt tgacggccaa tcctgcgtat tttgcagaca aggacggttt
     9901 gcatcactca gccggtctgg gtctcaagat tgccatgaac gaaaatttcg tactctcggc
     9961 cgattttggc aaagcattca acaaacaaga cggggcagga cttggaactt atataggcct
    10021 gaattacctg ttctaattac ataaatcctt aagatgaaaa cattgaaaaa actaattgcg
    10081 gaaaaattat tgaaaattaa ggctgtcaag ttgcagcctt ccaatccctt tacctgggct
    10141 atgggctgga aatcgcccat ttattgcgac aatcgtaaaa ctctttcata tcctcaactc
    10201 aggaatttta tcaaaataga actggctcgc atcattcgtg aaaaatacga acaggtggat
    10261 gccattgccg gtgtagccac cggagccatt gctcagggcg ctatggtagc cgaagaactg
    10321 tgcctgcctt ttgtttacgt tcgttcttcg cccaaggatc acggactgga aaaccttatc
    10381 gaaggtgact taaagcccgg catgaaggtg gttgttatcg aagacttgat ttcaaccggc
    10441 ggcagcagtc tcaaagcggt ggaggctata cgcaaggacg gcagcgaagt aataggaatg
    10501 ctggccatct tcacttatgg attcgatgtg gcaattgagc gtttcaagca agccaaagtg
    10561 gaattgacca ctttgtgtga ttacgatacc attttagaaa cggccctgga aacaaattat
    10621 ataagcgatg ccgatgtaaa gactttgcaa atctggcgca aagatccggc taactggaaa
    10681 cccaggatac aatagagaaa ccgatgaccg aatttaagac acagaccggt agaattgcag
    10741 ccccggcctg cagggttttt gaggttcttt ccgattggaa tcagctcgaa aagatcaaag
    10801 aaaagatccc tgagagtaaa ttgaggggcc ttgaatttga tgccgactcc tgtcggtttg
    10861 tgatagacag ctatggtgtg ggcgaaatcg ccttgtgtat ctcggaacga aatccctgtt
    10921 ctgtgatcaa gataaaggca gagcaatcgc ccatcgactt taacgcgatc atcagcattg
    10981 aagaagagaa tcccgaagtt tgcctgttga caatcggctt agaggctgct cttcccttta
    11041 tgttgaaaca tattttgtcg ccttccattc aaaccggctt ggacaaaatt cttaacttat
    11101 tgaccgaata ctcatattaa taactatgaa actttggcaa aaaaataccg aagtagataa
    11161 agacatcgaa cgttttacta ccggtaaaga ccgggagatg gatctttatc tggccgaatt
    11221 tgatgtgtta ggctctgcgg cgcatgcgcg aatgctggaa caaatcggac tgatttcgcc
    11281 cgaagaactt cctttgttgc tgcgaggatt acgggaaatt tacgaacaaa tacaggccgg
    11341 caagttcact attgaagagg gtgtggagga tgtacactcc caggtggagc ttatactcac
    11401 acgtaaactg ggagacctcg gaaaaaaaat tcacagcggg cgttccagaa acgatcaggt
    11461 cttgctcgat ctgaaacttt tcacccgcca caaactggaa gaagttctcg aagccgttca
    11521 tgccttgttt gagctgctta taaaattgag cgaacgttac aaaaaagtaa tgatgccggg
    11581 ctacacacat ttgcaaatag ccatgccttc ttctttcgga ctctggtttg cagcttacgc
    11641 cgaatcgctt gtagacgacg tacaggtgtt gcaggctgct tttaaaatat gcaaccgcaa
    11701 tcctctgggc tcggctgccg gctatggttc ttcttttcct ttgaacagaa gccttaccac
    11761 ccaattgttg ggttttgaag gcttgaacta caacgttgtg tacgctcaaa tgggcagagg
    11821 gaaaatggaa aagaccacgg cttgtgcttt ggcttcggtg gcaggtacta ttgccaaact
    11881 ggcttacgat gcttgtttgt ttagctctca gaatttcggt tttattgcct tgcccgccga
    11941 attcaccacc ggttcgagca tcatgcccca caaaaagaat ccggatgtat ttgaattgac
    12001 cagagctaaa tgcaacagat tgcaacaatt gcccgaaaac atttcggcca tcactcaaaa
    12061 tctgccttcg ggttatttca gagattatca gttgctcaag gagctttttc tgcctgcctt
    12121 cgatgaactc atagattgca tcaaaatgac ctcggccagt ctggagcggg ttcgtattaa
    12181 cgagcacata cttgatgacc cgcgctatga atacatcttc agtgtggaag aagtcaacaa
    12241 gagagtcttg aacggtgttc ccttcaggga agcttaccgg caggtaggta tggaaattga
    12301 agccggacgc ttcagagccg acaagaacat taagcacagc catgagggaa gcctcggcaa
    12361 tttgtgtaat gacaaaatta cagctagaat gaacgagttn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnt tcaacaaatg cggtgttcaa cctcagactg ttgtacaggc
    12541 tgttgaacgc cagatgtctt cttatcccaa agtaagcggc ggcgaaaact atctctccaa
    12601 agaggccaca gccgtgttgc aaaaagccac ggcttacgcg gctgaattaa aggatcagtt
    12661 tgtatcgctc gagcatattt tgctggcttt gctcagcgaa aaaagcccgg tttccaatat
    12721 actcaaagat gccggcttaa gacttagtga acttaagttg gccattcgtg aattgcgtaa
    12781 gggtaaatcg gccgacaccg agaatgccga ggaggtatac gatgccctga acaaatacgc
    12841 cgtcaacctg aacgaaatgg cccggaacgg caaactcgat ccggttatcg gcagggacga
    12901 ggaaattcgc cgcgtattgc aaatattgag tcgacgtacc aaaaacaacc ctatattaat
    12961 tggtgaaccc ggaacaggaa aaaccgccat agccgaaggt ttggctcatc gcattgtgcg
    13021 cggagacgta cccgaaaacc taaaaaccaa gcaggtttat tcgctcgata tgggcgctct
    13081 ggtagcgggg gccaaataca agggagaatt tgaagaaagg ctgaaagccg tagtgggcga
    13141 agtgatcgaa gccgaaggag agatagtgct ctttatcgac gaaattcata ccttggtggg
    13201 tgcaggcaag ggcgaagggg ccatggatgc cgccaatatc ctgaaacctg ctctggccag
    13261 gggggaactc cgggctgtcg gagccaccac tctgaacgaa taccagcaat ttttcgagaa
    13321 agacaaagcc ctcgaaaggc ggtttcaacc ggtgatggtg aatgagccgg atattgccga
    13381 ttccatttcc atactcaggg ggctcaagga acgttacgaa aatcaccata aggtcagaat
    13441 caaggacgaa gccgtgctgg cagccgtaga attgtcgtcg cgctacatta gtgatcgttt
    13501 tctgcccgac aaagccatcg acttgatgga cgaagcggcg gcccgcctgc gattgcaagc
    13561 ggattcggtt cccgaggaac tcgacgaaat ttcccgccgg atcaagcaat tggagattga
    13621 gcgcgaagcc atcaaacgcg aaaaagacga gcttaagctc aatcagttgg gcaaagaact
    13681 ggccgatctc gtcgaaaagg aaacagccat gaaggcagcc tggcaagcgg agaaatcaca
    13741 tatagatcgc attcaggaac ttaaagcaga aatcgagagt ttgcgttttg aagctgaaag
    13801 ggccgagcgc gaaggcgatt acggcaaagt ggccgaaata cgctatggct ccatacagca
    13861 ggccgaggcc gccatcaaaa aggaaagcga cgcacttaaa accttgcagc agagtggaca
    13921 gcttgtcaaa gaagaagtcg atgccgagga tatagccgaa gtggtttctc gctggacagg
    13981 cattccggtg tataaaatga tgcagagcga acgtatgaaa ttgttgcgtc tcgaggaaga
    14041 attgcatcgc agggtaatag gtcaggacga agcaatcaac gccgtagccg atgctatacg
    14101 ccgaagcaga gccggcttgc aggatcccaa acgccccata ggctccttta tctttttggg
    14161 cagtaccggg gtaggtaaga cagaattggc taaagccctg gccgaatatt tgttcaacga
    14221 cgaaaatacg atgatacgaa tagatatgtc ggaataccag gaaaagtttt cggttaccag
    14281 actcatcggt tcccctccgg gttacgtggg ttacgaccag ggcggacagt tgaccgaagc
    14341 agtaaggcac aaaccttatt cggtggtttt gttcgacgaa atcgaaaaag ctcaccccga
    14401 tgtctttaat attctgcttc aggtgctcga tgacggtcat ttgaccgaca acaaaggtcg
    14461 taaggtgaat ttcaaaaata cgattgtaat tatgacctcc aacctgggtt cggaactgat
    14521 acgggaacgc atggatcaga tgaacgaaag taaccgggat caattgatgg agcaaactcg
    14581 cgaagaagtg ctcgagcttt taaagcgaac cttgagaccg gagtttttaa accgcataga
    14641 cgaaattatt atgttcaaat ccctgactcg ctcggagatt aaggaaattg tgcgcttgca
    14701 actggagcaa cttaagagta acctggccga gagaggcttg cgtatgagat acaccgacaa
    14761 actgctggat cacctggcca aggaagctta tcatccgcaa ttcggcgctc gtccggttaa
    14821 aaggctgatt caaagagaat tgttgaattc tttatctaag gctttattat cgggcagcat
    14881 tcaggaagac agtctggtcg aaatcgacga aagcggaggc gaaattcttt tccgctcgat
    14941 tataaatccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ctgcaatctt
    15061 taatttttta attagttttg cacagtaaca aaaaaacaaa aatatgctga ttaaacgatt
    15121 tgtattaagc ttaatgcttc tgaacttgtc ggggctgtta ttggctcaga ccgatgtgag
    15181 cctgttttgg agaaaagatt ccctgcaaca aacagaaatt ttttctgccg aaggcgattt
    15241 atatcgttta gtgggtcacc acgggccggc tatagaaaat aaatttatgg ctttacgcct
    15301 gtatttcaat cacagcggat cgatcgatgt atattccaag aaaatacatc gtctggaact
    15361 ggcgcaggct ctttggtatc cgtccgaaaa gatgcagcag gaacaaggtt tcggttgcga
    15421 tgagtacaaa gtgggttcta ccgttggctt ggggggtatc aatctttggg atggtcggca
    15481 agttgtaaag ctcacagcca cccggggccg aaccgccagg gtttccaaaa acgaaaaggg
    15541 agccagtatg gaaatgctgg ccaaaggcat agcctacaaa gacaaaatgg tagatattct
    15601 aatacgggta agtgtcaaca acacagacag acaggccact gttgaggctg tttgtacttc
    15661 gggacataaa gtacagtttc ttacgggggt aaaccgtcac ccgggcaaca gtgtcttcaa
    15721 ccgggacggc cggataggcg tctggggcag acacccggcg gatgtttcca aagctcccgt
    15781 tttgataggc ggaggtatga aataccgtcc ctccgatttc gctattaagc gagtaacaga
    15841 cgacgccatt cagctcatat ccaaaccggc taaaaagctt aaaaccacca ttgtatctgc
    15901 gggagagcgg gaagaagagc tgaataccga gcaggctttt ttcgattatg taaagagttt
    15961 ctgatctagg cggaatcggg agtttgtatc actctgtttc atagaattct gtcattaaag
    16021 catcaagccc ggcaaatcga taattgccgg gcttgatgct ttaattggaa tatttccgaa
    16081 gaaatttatt tcttgttgcg gttgccgtag cggttcagga atttatccac acgaccggcg
    16141 gtgtcgacca acttggattt accggtataa aaagggtgcg aagtggaaga aatttccagt
    16201 ttgaccaaag gcagggtttc gccgtctatt tctatagttt ctttggcttg aatggtggaa
    16261 cgtgaaataa aggtgtcacc attggacata tctttgaaaa caactggacg gtaattcgtt
    16321 ggatgcaaat cttttttcat tgctcaaaat attttggctg aatgcgtcag ccgggtttta
    16381 agattataca aattggtaag atctgtaatg gcctgcaaaa gtagatattt ttaatgaaac
    16441 caaaaaaaat atcgggcttt ttttctcatt aagcgcaaaa taccgtaatt ttgtaagctt
    16501 agagataatt aaatgatcaa aatcctttaa atacagatat tatgattagt tacaaagaat
    16561 taggtcttgt caactcaaga gagatgttca agaaggccat agccggtcaa tatgctatcg
    16621 ctgcatttaa tttcaacaat atggagcagt tgcaggccat tattcaggca gccgtcgaaa
    16681 cccgttctcc cgtgattctg caagtgtcca gcggtgcccg caagtacgcc aatcagactt
    16741 tgttgcgtta tatggctcag ggagcagtag aatacgcaaa agaacttggt ctggagcatc
    16801 ctcagattgt gctgcatctg gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16921 nntagaatac gcaaaagaac ttggtctgga gcatcctcag attgtgctgc atctggacca
    16981 cggcgatact ttcgaattat gcaagtcttg tattgatatg ggcttttcct ctgttatgat
    17041 cgatggttcc catcatccct acgaagacaa tgttgctttg accaggaaag tggtggatta
    17101 tgctcataaa tacgatgtta ccgtcgaggg tgagctgggc gttttggccg gagtcgaaga
    17161 cgaagtttct tcggcagtca gtcattacac ccgccccgac gaagtggtag attttgttac
    17221 cagaacaggt gttgattctc tggctatttc tatcggaact tcgcacggag ccaataagtt
    17281 cacccctgag caatgcactc gagatgccaa tggtgtttta attccacctc ccttgcgttt
    17341 tgatattctc gaagaaattg aaaagcgaat accgggtttc cccattgttt tgcacggttc
    17401 ttcttctgtg cctcaggatt tggttgccat tatcaacaaa tacggtggcg ccatgaaaga
    17461 ttccatagga attcccgaag aacaattgcg tagggctgcc aagtcggctg tgtgcaaaat
    17521 caacatcgac tccgacggcc gtattgccat gacggcagcc gttcgtcagg tgttggctac
    17581 caaacccgcc gaattcgatc cccgcaagta cctgggccct gcccgcgatt cgctcaaaga
    17641 attgtacaaa cataaattga tcaatgtttt gggttcggcc aataaactat aattcaataa
    17701 gattcaaagc cgaaacaaga ttccaagccg aattgaatcg gcgtaaaaaa gcgagcctgt
    17761 gctattcaga caggctcgct tttttacatc cggcacctag tagaccgaac tcccgatcag
    17821 ccgcaatttc tcttcagtaa agtgcaaatt atccaatgcc tgtatgtttt cactcaactg
    17881 ttttaccgag ctggcgccta ttataacgga agttacccgc ttatcgcgca agcaccatgc
    17941 caacgccatt tgagccaatg tctgttgtct ttttcgggcg aagtcgttta gttgcttgat
    18001 tttttctatt ttttcggggg tgatttgctc ttttttcaaa aaaccaaatt cttgtccggc
    18061 tcgtgaattg ggaggtatcc cttccaaata tttattgctg agcattcctt gtgccaaagg
    18121 ggaaaaaccg ataaaaccga tgtttttctc ttccaccagc ggaaaaatgg attgctctac
    18181 ttcagggttg aaaaggctgt atctgccctg ataaataagg caaggtgtac cgttttcgct
    18241 cagcaaacga atggcttcaa gggctttgtc gggcggatat tttgaaatgc cgacgtagag
    18301 ggctttgcct tgctttacca tatcggacaa cgcacccata gtctcttcca gcggcgtatt
    18361 cgggtcgtag cggtgcgaat agaaaatatc cacatagtcc aatcccatcc gtttaaggct
    18421 ctgattaagg ctggcaatca tatatttacg cgaaccccaa tcaccgtaag gaccatccca
    18481 catcaagtgt ccggctttgg tgctgataat cagttcgtct ctataggatt taaaatcatt
    18541 tttcagcatt ttgccgaaat ttgtctcggc acttccgaag ggagtaccgt aattattcgc
    18601 caggtcgaag tgagtaattc cattgtcaaa agccgtaaac aacatttttt tggcttcgtc
    18661 ttggtcgttt acatcaccga aattatgcca aagtcccagt gaaattcttg gtagttgcag
    18721 tccgcttttc ccgcaataat tatatccgac tgagtttaca tatcggtcgg tagaaggggt
    18781 atattgcatt ttattggaag tcttgcggat aatcataaaa tctcttatct gtttttaata
    18841 cgaactatag caccggtttc gggatcgagt tccaacatca actgctcaga agccggtaat
    18901 tgcatcagat cgccaaattg cgctatcacg aaattgtctt tgaataaaac tgtctggcct
    18961 ttcaataaac ttatgtcaat tctgttgggg tgatagtaat atagagcgca ggtttgttgt
    19021 gatgtttcac cttttttacc actgatgctg tccttttgtt ggcttatggc gtcggtaccg
    19081 ggattgtgta ccaaaaaatg taccggatac ccgctcaggt cttctttgtc tactatccct
    19141 ttttgagacg aaaagcgaaa caaaatttcg taactgggaa tcttttcggg tgtatattca
    19201 agcacctctg ttcggatgct ttgtctgcgg cttccggtaa aaagttccgt ataatgcgct
    19261 tccatgcggt ttagttcggt aagcatttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19381 nnnnnnnncc ggtaaaaagt tccgtataat gcgcttccat gcggtttagt tcggtaagca
    19441 ttttttcgag cgaagctcca tcctgaggca tagtgctcaa ttctccctgc aataggctga
    19501 gccgggtgtc tctcaattca aagacttttt tggcggccaa ttcggccatg cgaatggaag
    19561 agccggaaag ttgcatatcg tgggtatgaa tagaggctgc agagctggcg ctgttgtcgt
    19621 ttgcgttttc cggacaagaa attgttgttt cctgttttga ggctttcccg gaacaggatt
    19681 cagttaagct aaggtccata tttacagccc tgagaattcc ttcggaggtg aactgaagat
    19741 taagttcagg aacttgtgtg tctgacgctc tcctttttga gacctgaggc gctgcagctt
    19801 gtatgtaaaa aactctttta gggtcgggca gggtataagt atctaattcc accgatacca
    19861 gttcgttgtt tactctttct gtagttacaa tatcgctgat cccaagcaat ctttcggaat
    19921 atctggcata aatgccgggc tcttcgtgtg tggttctgat cgtgacacga accttgaaca
    19981 cagtttttgg aagatgatag ctgaaagctt cgtaagggat gggaccttct acaatacgac
    20041 tttgaatctg cgcagtaagg ttaccggata aaccgagtac aacgagcgat aaaaacagca
    20101 gttttttcat aaatatataa gttcaaattt gtcctttact atgtttgaag gctttaccaa
    20161 gatactgaat caggtctccg gctacaaggc tttcttccga ttgctctttc agggccaggt
    20221 ctccactcag gccatgcaga tatacaccaa gcaggcagca ttgagccgga gggtaagcct
    20281 ggacgagcaa agataataaa attccgcaca gaatgtcgcc actgcctgcc gttgccattc
    20341 cggaattgcc ggtgttgttg aaatgacgac tgccgtccgg gaagatgcta cacgtgtaat
    20401 gccctttcag aataaggtat accccgtgtt ttttagcaaa agcgctagct ttttcgagac
    20461 gttcataccc gcaatccgaa gctccggcca gccggtcaaa ttccccggga tgaggagtta
    20521 agatggatcc ccggggcaat aagcttaata gggaaggttc ggcagcgaga atattgagac
    20581 cgtctgcatc aatgaccaaa ggttttcttg tttctttcaa caaggccttc atgacccgag
    20641 tttgaccctg tcccgttccc aggcccgggc ctatggcaat ggcagaaaaa cggtcgagat
    20701 ccaaattttc catggaggaa atattgtttt cggaaagcgg atcgatactt gtcatggctt
    20761 ccggaacggc gctttgaatg caagcagaca aggccgaagg cattctgatg ctcagcaggc
    20821 caagtcctgc cctcaaggcc gctcgccctg ccagaatagc cgctccgggc attccggcgc
    20881 tgccggcaat tagcagaccg tgtccgtaat tccctttgtg gctgaatttt tttctgtttc
    20941 gcaaaacggc tctgatgtcg ttttgttcta ccagcgaata atttgttgtt tgacgggcaa
    21001 gagcttcggg atgaaaatcc agcggagtca gctcccaacg acctatataa tcttcgtttt
    21061 cgggcagtaa aaaagccagt cgcgggaatt ggatacccag caccagatct gcgcgtacta
    21121 tattttcggg tttgtttgtc cggttgtctt ctccgtacag gcccgaaggc aaatctatgg
    21181 aaatacgcag ggctttttgt ttattcagcc aatgtacaat ccgggcaaaa tccccttcca
    21241 gcgctttgtt tagtccggag ccgaacaatc cgtctataag gtaatccgta ggagcctctt
    21301 ttaccaaatc ttccggcgtg gcatcaaata cgaatgatga agggaacaac tcaatcagtt
    21361 tttttgcgtt caaagcgcaa tcgtgcgaaa gtgttttgtt tccgctcaat aaaagacatc
    21421 tgagttgaaa ccccgcctcc agcatcaaac gggataaagc cagggcatct cctccgttgt
    21481 ttcccggccc ggccagagcc agtatacttt tattgggggc aatcagtgtt ttgattcgct
    21541 caaaaaggcc tagtgcagct ctttccatta gttcggaaga atcgatacct tcgatttgaa
    21601 ttgtgtcggc atccagttgt ctgatttgag ctgtggtgta tatattaagg gccatttttt
    21661 ttacgattat ccgtatattt atccaaaaat ctgtacaaat ttaatacaaa agtaaaaaat
    21721 aattcccgag caatttccag acagccattt ttttgtatat tcgcaacttc gttgtattct
    21781 ttctgaaact catgaaaaca gttcaggtaa aagaccggag ctttgcttta cttcttactg
    21841 ccgaacgtat ccggaaaagg gtttccgaat tggcactgga aataaacaaa gatatggagg
    21901 gtaagaatcc gctgttttta gtaatgctca acggagcttt tatgtttgca gcggatttac
    21961 tgaaggaaat taatgttccc tgcgaaataa gttttattaa atacgattcc tatcgtggaa
    22021 cgaaaagcag cggaaggctt aacaaactga ttgggctcaa agagtcgtta cgtaaccgat
    22081 gtgtaatcgt tttggaagat gttgtagaca gcggtttgac tatgagccgg ttgtttgaag
    22141 aattgaaaac aaagaaaccc gctgaattat acctggccac ctgcatcttt aaaccggagg
    22201 cattgaagtc tgaggttaag ccggattatg tgggcttcaa tttaccggat gtttttttgg
    22261 ttggctacgg tttggattat gaaggttacg gaagaaattt agaagccatt tatgagttga
    22321 agtcccgtta agttgagatt attaggtggc atatagagga taatttttaa agaaaatgct
    22381 gaacattgta atttttggag gtccgggatc gggaaaaggg acccagagtg cattaatagc
    22441 cgaaaaatat aatctgatac acctgtccac gggagatctt ttgcgtgacg aagtagcaag
    22501 cgggtcggag ttgggcaaat taatccactc ttttattgac aaaggcaatt tggtacccga
    22561 tcaattgatg ttagatattt tgtcaagaac catcgataat ttaaacggca aaaaaggggt
    22621 gatctttgat ggatttccca gaaccatagc tcaggctgaa gctttggatg aaatattgga
    22681 aaaaaggaac gaaaagctga acctgttgtt gcatttgaaa gtggccgacg aaattttaca
    22741 agagagaatg ttgtttcgaa gcaaaacttc cggacgttca gacgacaccc ctgaaaccat
    22801 caaaaaacgt atagatattt atcatcaggt tacggctccg gtcataaatt attacagaca
    22861 tagaggctta tatcatgcca taaaaggaaa tggaagcggc accgtcgaaa cgatatttgc
    22921 cgaggtcagt agttacattt cggcttatca gcaaaagaga taatggcaga aagtaatttc
    22981 gttgattacg taaaaattta ttgccgttcc ggtaacggcg gccccggctc aactcatttc
    23041 caccgcggaa gatacatccc caagggcggc cccgacggtg gggacggagg caggggaggg
    23101 catattattt tgcgtggaaa ccggaattac tggacgctga ttcatttgcg ttaccaaaga
    23161 catatttttg ccgaacacgg agaaccgggc ggcaaatcca gaagcaccgg acgctccggt
    23221 aaggatacca taattgaagt tcccctgggt acagttgctt acgatgccga cagcgaggaa
    23281 tttctttgtg aagtgaacga agacggggaa gaaatcgtac ttctgaaagg aggccgtggg
    23341 ggcctgggta attggaattt caggacggca gtcaatcaag cacctcgtta tgcacagccc
    23401 ggagaaccca tggaagaacg tacggtgatt ttggaattaa aactcttggc cgacgtgggc
    23461 ctggtcggtt ttcccaatgc cgggaaatcc accctgcttt cggtattgtc ggccgcaaag
    23521 cccaaaattg ccgattatcc ttttaccacc ctggaaccca atctaggcat agtctcttac
    23581 cgtgacggct tctcatttgt gatggccgat attccgggaa taattgaggg agcctccgaa
    23641 ggcaagggaa tcggtttgcg ttttcttcgt cacatcgaaa ggaattctgt cctcttgttt
    23701 atgataccct gcgacagcaa aaatatcatc gaagaataca ccatattact caacgaattg
    23761 aagcaataca atcctgaatt gctcgataag gccagggtgc ttgccattac caaggcagat
    23821 atgctcgatg cggagctcag agccgccatc cgcaaggaat tgcccgaaat cccttcagtc
    23881 ataatatcgt ctatagatca gaccggctta accgagttaa aggatttgct ttggtcggcc
    23941 ctgaatcaat aaagaacctg tttttaaagc gattagtcaa ttgtttttca gcgattctta
    24001 aaaacagacc caatatacta acaaaacatt aaataaatat cattcaaaag agaatttaaa
    24061 ccaaaataaa cagtatattt gccgcgaatt aaccggattt atctggtact atgtttaact
    24121 taaaaattat actgttatga gtttatttat ctcttacaag cagaaacggg gccgtttttt
    24181 atcggcactg ctgtgctcat gtcttgtttt cttaccggta aaagcccaat cttcaggtgc
    24241 ttatctctct gaagccaaca atttgttgtt gaacacgggt tttgaaattt gggaaaacaa
    24301 tttacccgcc cactggtttg gttctgccag caacattccc gaaacttcgg tgtttcagtg
    24361 tgctgaagcg cattccggtg agtcttcctg tcgtttggtg cgtacccaaa aaacccatgt
    24421 gcgctttagc tctgctcctt tatttttaaa cctcggtaat tacaggttaa gttattatgt
    24481 gaaaggaagc ggctgtattc gcaacagctt ttacaacggc agttcctacg ccgcctattc
    24541 cgactacgac accctgagca atcaacagga atggatgaag aaagtctatg aatttgaact
    24601 caagaaagat gtagaagtgc aattgttgtt ttcgctttgt ttaaccgatt cgataggttt
    24661 gtttatcgat gacgtcctat tcgaaagcat tcctgccgga ctcaaccggc ctgttgagtt
    24721 ttctccttta attcggacag ctcagggctt gctccatctg caattatcga atccacaggc
    24781 attagtagtg tatgatatgc tcgggcgaaa agtctgcgaa agaaatgttc agggcgattt
    24841 gagtattcct ttgtcgcgtg gttattatct gattgcatta tccggtcacc caaagcccgt
    24901 taaggtgtac attccctgat ttttgtttta attggctgtg catcaataac cggagtgttg
    24961 ggtgcaggct ttattatatg taaaacatcc gccaaactgc ttttcgcaag tttggcggat
    25021 gtccttttgg tggagccgga gggcatcgaa ccctcgtcca aacggaaaac caataggctt
    25081 tctacacgct tatcttcgtt tagtttttcg acccggggca aaaccgaagc tatctacccg
    25141 gagcttatct ccttaaattt cggccgggaa ccggagcctt tccctgccta tttccgatat
    25201 tgctgcacca cctggtccgg ccgcttcgga accacagcac cggggtgatg acccgtttct
    25261 gccactgtga cagaaattaa gcttagatct actatacttc gattaagcag cgagtgcgta
    25321 attgttttcg ccagttattg gtttggtaag gatattaaag tgcgtattac catagcactg
    25381 cgtgcttacc tacctgtttt gaccgctgtc aaaaccaatc ggccccctat tctattttgc
    25441 ataactcacg gctctggtct cacggataac cgtgattttt acctgaccgg ggtatgtcat
    25501 ttcatcctga atacgtttgg ctatctctgc agaaagctgt tcggtattct tgtcgtccac
    25561 tttttcggca cccactatta ctctgagttc ccgacctgcc tgaatggcat aggtcttgat
    25621 gactccgggg taggaaagtg ccaattgttc aagatcgttc agccttttta tgtaggcttc
    25681 tacaatttcg cggcgggctc ccggccttgc tcccgatatg gcgtcgcaaa cctgtacaat
    25741 aggagccagc agggtttgca tttcaatttc gtcgtgatgc gcgccaatag cgttgcaaat
    25801 atcgggtttt tctttgtatt tctccgcaag tttcatgcca agaagtgcgt gcggcaattc
    25861 cggttcgtct tcgggtactt tgccgatgtc gtgaagcaag cctgctctcc gggctttttt
    25921 gggattcagt cccagttccg aagccattac ggcacagaga ttagcggttt ctctggcgtg
    25981 ctgcagcagg ttttgtccat acgaagaacg atatttcatc cggccaatca ttctgaccaa
    26041 ttccggatgt aaaccgtgaa cgcccagatc gatcgtggta cgtttgccag tttcaacgat
    26101 ttcttcttcg atttgtttgc gtactttggc tacgacttct tcgatgcgcg cagggtgtat
    26161 gcggccgtcg gaaaccagtt gatgaagtgc gaggcgggca atttctcttc tcaccgggtc
    26221 gaaagccgaa aggacaatgg cttctggggt gtcgtctaca atgatttcca caccggttgc
    26281 ggcttctaaa gcgcgtatgt tacgaccttc ccggccaatg atcctgcctt tgatttcgtc
    26341 cgaatcgatg tgaaaaacac tgatggcatt ctctatggcc gtttccgttg caacgcgttg
    26401 tatcgattga acaacaatac gtttggcttc tttgttggcc gtcattttgg cctcgtccat
    26461 aatctcgttt atatacgaag cggcgtctgt tttggcttct tctttcagcg attccaccag
    26521 tctttctttt gcctcctcgg cggataatcc ggacaaagct tcgagacgtt caactccttt
    26581 acggtgttgc ttgtcgagtt cctgtttttt cttatcgatt atttccaatt gtgcttccag
    26641 gttctcgcgg atgatttcaa gctcgttttt cttgcgttgc agctcatctt gcttttgatt
    26701 cagctgcatt tctctttgct tgactttgtt ttcgtacgat tggactctgt tgctcctttg
    26761 tgtgatctgt ttttccagtt cggctttcat tgcaaaaaat ttgtctttga tttcctgcat
    26821 tttattcttg cgaatagcct ctgcttccag ttcggctttt ttaattttgt agaggtattt
    26881 tccattcagg atataccgga aaatcagcca tcccaggaag atacccaccg acaaagccaa
    26941 tactaaataa actatataca ttatatccat agacgtgtgt attagataaa ataaaaaaac
    27001 actacaatac caaggtcaac accttgatag ctgtagtgcg gaatttgtag tctcagttga
    27061 attatataat ttctcccaac tctgcatcca gtttagaaat actttccatc aagatgtttt
    27121 ggtcagaaga tttttcaaat tctaattttt cgaaagcaaa atggcaggct accatagcta
    27181 ggatatcttt aatatccagg tctgatcccc tgatgccaaa tttattccgg tattcggtca
    27241 ctgcatcgtt aaccaattta gctgctttgc gataagcttc ttctttgctt ctttttattc
    27301 gaagcggata atacttttcg gcaaggtata attcgattct aaaagtatca tctttcattg
    27361 ctatgattca ttaacgagtg caatgcatgt attgatctcc cgcactaatt ttgacaggcg
    27421 ttgtttggta gctttgaggt catcgtcatt ggctgacatc atttttgcta acttaagtct
    27481 gtcgtatttc tgtgagatat cccgaagctc ctcctgaata ttgagaaaag acagttcctt
    27541 ttgcaataag gcttctttaa gtgccttgtt ctcttccctg agagttttgc agagtgcgat
    27601 ggcttgccgt actctggctt cgaattggat tagcaaactt tgttgttcct ccgtcataac
    27661 ccaccgaaat tgtctacaaa agtaaacttt agttttgcca aaaacaaata aaattcaagt
    27721 tttttatatt ctgtaaaggt cttctgttca taggggtttt ataaacacac aagccccggc
    27781 gagatattcg ccggggcttg ttatagtgta tttaagctat agagttagct taaaatatga
    27841 gggtcttatc agagaaccac cttgatggct tccgaatcta ttttaaccac atagatctgt
    27901 ccctgagaga gtccttcgat ggtgttgagt ccctgttcaa gtcttacagt tcttaccaaa
    27961 gcacccatca cgttgtagat gttgatgttg gtttgttctg ttgcctgaac atagaggttg
    28021 cctcccatag taaagactac gttcttttgt actccgtctg tttgaggcag agcatcgtaa
    28081 ggatcgctcg gaacttccat acctgccagc atgtagagag catttctcca gatggtaaag
    28141 gcagcattgg tcatgttggt tccgttatcg gcacacatgg caccgaagtt gaatccaacg
    28201 gtaatcatcc gttcggccag tgtttctccg cccacaacgg tacctgcagg caggtcgttg
    28261 ataaagatca cggcgtcggt tacaccggtc gggatagcga tcggggtgtt ctcaccggag
    28321 agcaccactt ctttggcgta gttgagggcc ttgatagatt tctctgcacc caatgtacca
    28381 tcgtccattg caggagcttt gaacagtgtc agggtgttgt cgtcagcaaa cgtcaatccc
    28441 ttgaacaggt cgttgccctg ttgtgcggga tcaacggtaa tgctcagacc ttgctgttct
    28501 gcacctgaac ccggttgtcc gctggccaga gctctacctc ccctaaaggc ataggttttg
    28561 ttatagagca cgggcaggtc aaacttggcc aatcccagag gtcctgcggg ggtgaggatc
    28621 ttatcaccgc caccaaagga ttcctgaacc agagcgatgt cgtaggcatc caaatcgacg
    28681 ggcgaagtgg ccgaaacatc ggtgagaaca atttggtcaa caataaagtt gtcgtcggcg
    28741 gccatcatca gataaaccgg atcgttttct acggtagcag ccgtggcagc catagtcttt
    28801 tgcttggtaa ggaaagcaat gacgggggtg ccggcgttac caacgtagat ttgttcaggt
    28861 ccgataaagc ttccacctgt tccggcttca aacagcggag actgcttgga gatggtcaat
    28921 ctggcttcgt ttctgaagac tttgctcaaa ccttcgaagc tgtccatgaa caggtcgttg
    28981 cggctgaatg taatatcagt ttctgccggt cccaccaatc tcaggttcgg atagttttcg
    29041 ggagtggatt catccttcac aacattatcg ggatagaaga agttgttcaa gacattcagt
    29101 gtgactgact gtccgggtgc cggcaaaaac atggttagct tggcatgcgg gaaaccggcc
    29161 tggctgcctc tttcgtagaa gatgttgttg ttgatgttga taacaccggt caaaccaccc
    29221 agcgacttgg tgtattccaa gaagtttctg acgttggttg cgtttccgcc aaacttgtag
    29281 aacaagttgt tttcgatagt aacaacaaag tttacatcgg tagagtcggc tgtcgtacca
    29341 tctctgttga tgttgaacac ctgactgttg cgccagttgt taacgatgtt gttgcggaaa
    29401 gtgaagtggt caaactcata ggtcttcttg ttggaaagtt ggaacatatg gcccgaatgc
    29461 tgagccattg tatcctgatt gacacccaca tccttaatca cacaatgctc cataacgaag
    29521 tcctttatga ctgcgtaatt ggcaatcttg ttggcggaaa gaatcaactg tttgaatggc
    29581 aagttcgtaa acaagcaatt gcgaacattg aatcctacca tgacagagtc gataccagtc
    29641 atatacaaag gtgaataagt atctgtgttg taggcatcat ataccgactg agcgttaaac
    29701 tcaagtcctt cgtagagcag tgtggctgcc ttaactccgt tgttggaggt caagcgtccg
    29761 gccaggacgg gattttgtcc ctcagcattc ttgatggtga ggtcaccacc tgtttgcggg
    29821 aaggcaaatc ctgaattacc caagaggtaa acaccgcctt cgtcggtaca attcatcaac
    29881 tcgatggtga tgtctgccga ttcgtaatcg gtgtatgcca aagcttcggt aagttcagcc
    29941 aggttcttga caatagcacc ggtgggctgc tcaatgaaag caggcacata gtaggagctc
    30001 aacgcaccaa cgtatgttcc ttcggtaccg gcggtgtaga gaggcgactt ataagaaagc
    30061 agttctccgg gaaccaatac cagacccggg aagtcgctca ggaacaggtc gttcctgttg
    30121 tgatcgataa ccggatca
//
LOCUS       DAOSTD010000015        29510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1732_length_29510_cov_14.550025, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000015 DAOSTD010000000
VERSION     DAOSTD010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29510
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1732_length_29510_cov_14.550025"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLI37_01850"
     CDS             complement(<1..189)
                     /locus_tag="PLI37_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57794.1"
                     /translation="MILYWKNGAKVHKLTKSTKTGLHLFVIPRENTTFGQQISGTMDN
                     SLLDVLVIVGAVLISVIGA"
     gene            188..1669
                     /locus_tag="PLI37_01855"
     CDS             188..1669
                     /locus_tag="PLI37_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HOQ57795.1"
                     /translation="MSLNFITAQEAASFINHNDNIGFSGFTPAGAPKVIAQAIAEKSQ
                     AERQAGREFKVGVFTGASTGDSLDGVLARAEAVKFRTPYQSNPDLRSGINKGNIPYFD
                     LHLSQLAQELRYGYYGKINTVIIEAAGVNEKGEILLTTGVGITPTAARMADKIIVELN
                     SRHPSFINGLHDLYEPADPPHRREIPIYKPSDRIGQTLLKVDPSKIVGVVKTQLPDEV
                     KAFSEPDETTKRIGDRVAGFLVSEMRAGRIPASFLPLQSGVGNIANAVLGSLGASPEI
                     PAFEMFTEVIQDSVIEMMQSGDVKFASGCSLTITSSALDSIYENWDFFKHRLVLRPQE
                     ISNSPELARRMGLITINTALEADLFGNVNSTHVLGTKMMNGIGGSGDFTRNSYLSIFT
                     CPSTAKDGKISALVPMVSHTDHNEHSVKILITEQGVADLRGKSPMQKAQTIIENCAHP
                     DYKQLLTDYLKQTNGGNTPHNLGNCFNMHREFLDSGDMRNTKW"
     gene            1671..2204
                     /locus_tag="PLI37_01860"
     CDS             1671..2204
                     /locus_tag="PLI37_01860"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF010555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOQ57796.1"
                     /translation="MIRLFLVGYMGAGKTKLGQAIAKELQLSFVDLDQYIEERRFKPI
                     PALFAEFGEEGFRKIEQGYLREVAQFENVLIATGGGTACFENNMDFMNSAGDTVYLQL
                     NAEELFKRLHRKKQNRPLLNTVSDDDLLTKIREMLSQRESFYNKAKYILPTGSLNKEE
                     SLTLFKQIYSKPPSPTI"
     gene            complement(2178..3713)
                     /locus_tag="PLI37_01865"
     CDS             complement(2178..3713)
                     /locus_tag="PLI37_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOQ57797.1"
                     /translation="MYVKLISAAIQGIGAIPVYVEVHVSRGIRFSLVGLPDNAVRESH
                     ERISSALQYCGMDIPRKQVVINMAPGDLRKEGSGYDLPLSVGLMAAGEYVPAESLKDC
                     LFIGELSLDACLQEVRGVLPIALTAREMGLKRLILPHKNAAEASVVEGLEVYGMKHLS
                     EVVAFLKGESRPEPYCSRLDFFDGAQVVPEVDFAEIKGQERAKRAIEVACAGGHNLVM
                     IGPPGSGKTMMAKAIPGILPPMSLDEALETTKIHSVAGKIDARCSLLTSRPFRSPHHS
                     ISSVAMIGGGSNPQPGEISLAHNGVLFLDELPEFQRSVLEVLRQPLEDRSISIARSRY
                     AVRYPAGFMLVASMNPCPCGYFNHPTKECVCTASQVHKYIHRISGPLMDRIDIQMEIV
                     PLEYEQLSSVREQESSQSIRERVIKARRLQNDRFKPDKGLFCNAQMSLKQVQRYAQAE
                     PDAYVLLRNAMEKLDLSARAYSRILKVARTIADLDASERLRTRDIAEAIQYRSLDRGA
                     WGF"
     gene            3981..6131
                     /locus_tag="PLI37_01870"
     CDS             3981..6131
                     /locus_tag="PLI37_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOQ57798.1"
                     /translation="MKKLPLFIFVLFTCSVFNLLAQLPYTNPALSHEARAQDLLGRLT
                     LEEKVALMQDQSPAVPRLGVPEFGWWNEALHGVARSGYATVFPQAIGMAATFDEDAVF
                     RTFTAVSDEARAKATHSKQVEGRRGKYKNLTFWTPNINIFRDPRWGRGQETYGEDPYL
                     TTRLGVAVVKGLQGPDDSHYAKAMACAKHYAVHSGPEWNRHSFDAKNIRPEDLWETYL
                     PAFKALVQEANVKQIMCAYNRYEGDPCCASNSLLVDILREGWNYRHVVVSDCGAINDF
                     YNRGRHETHPDSATASGAAVRAGTDLECGRSYRSLVGAVNKGLIAESELDVSLKRLLK
                     ARFELGDFDPDSIVPWTRIPMSVVNCEAHQQLALEMARKSLVLLKNDKNTLPLPKKAG
                     GILVMGPNADNESMQWGNYNGTPYHTITILQGLEAALGASLPCVRTPHVVTEDKVQFT
                     VDSLMKDLPACDKVLFVGGISPALEGEQMRVSLPGFRGGDRTTIELPAVQRALVKALA
                     EAGKQIVFINCSGSAVGLSEEAKICDAILQAWYPGEKGGQAVAEVLFGDYNPSGRLPV
                     TFYAGDHQLPDFEDYSMEGRTYRFLEEKPLYPFGHGLSYTSFAYSKPKIKKGTKTLSV
                     TVTNTGKRSGEEVIQLYVQRTAPQARGKALRGFRRINLGPGESKEVSFSLSGGTLDFY
                     DSNQAIVTTLPGTYLLEVGGSSDPAACVRTSYTL"
     gene            6151..>6349
                     /locus_tag="PLI37_01875"
     CDS             6151..>6349
                     /locus_tag="PLI37_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57799.1"
                     /translation="MKLYLSILFFLFSAAMLFAGPSCDLKYYTGADGFRNSSAIDILQ
                     AKNGLIWISTRNGLTLFDGYSF"
     assembly_gap    6350..6449
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6450..>8827
                     /locus_tag="PLI37_01880"
     CDS             <6450..>8827
                     /locus_tag="PLI37_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOQ57800.1"
                     /translation="IVFYGPDKGIRGENIHWIAQDREGDEWVLTNRGVSVIGKKTLTS
                     HVEFHYFLEKGDYIWLASNDGKLARYSFEQDSLEFINLPSSTTSFHFMGAMKGDTIAL
                     GTNKGLLLINSCDKTHKHIPVDLIPAGNAGISFVYQDAYNDYWLFAENGVYRYEQVTS
                     EIRFFQSPDVYLQHEYPNKSFAYEDPYGHLWLIPGNGNFCYFDREQQELRYHYSEPGD
                     ASTLISPSVRHFLFDRQGNIWIGTNNTFGKLSFYESTYRVIPAESDEFEVRALFKDSR
                     EVLWVASKHHKLKLLDENYAKIDYRLYDANTHRELRIDFNIYSIYEDKAGNIWLGSRS
                     RGLYLLKRDEQNPYAFAVQHFIHDPADSMSLSDNSIYSIFQDSRKRIWLGTYGGGLNL
                     IREDASGKINFIHSGNSLKNYPEVYAQIVRYITEVDNTLLVGSMHGLFSFSSAFESPE
                     EIRFSRNARNEYIAGSLSNNDVMYIYSDSENQVYILTQNGGVNKLVSGNLLSDNMVFE
                     VIGEREGLASDLTLSMTEDPSGKLWIASKQDLSRYNPSGGLINNFGSYYFKQDFVFSE
                     SKVINTSKGSILFGTNKGILEINPSTMSGSNYVPPLVFTEIKLNNQAASVPIDQLKDI
                     DLKSNQRNFSIQFSALDFNNSKEIRYAYILEGLEKEWHYVNDNRSAEYIKIPKGRYQF
                     VVKSTNSDGVWVNNERKLDITVLPTFWETPWAWLLYIFFFILLVSSIVYVLFIIYRLR
                     HEVTAEQQLSEIKLRFFTDISHELRTPLTLISSPVTDVLEHEKLSETARENLT"
     assembly_gap    8828..8927
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8928..9892
                     /locus_tag="PLI37_01885"
     CDS             <8928..9892
                     /locus_tag="PLI37_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ57801.1"
                     /translation="AKIEVLSEVGKGSSFRLFFPLGSEHFANNPQVEFLLSDRQSDVN
                     LPSEEQSQTGQEDDVQDRQSILIVEDNPELISFLHNILSRDYTVLQATNGLEGWNKTQ
                     EMLPDIIISDVVMPVMDGLDMVKKIKENNNTCHIPIILLSSKASLDDRIKALERGIDD
                     YITKPFSASHLITRVQTLLEQRKFLQDYYLNKFSKNRTSDADLSSESPAIASYDEQFM
                     KQVIDYIEQNIDNVLLVVEDLAKEFPMSRSVFYKKIKSLFGLSPVDLIRTIRIKRAVQ
                     LIDSSTHSFTEIAYMCGFNDLSYFGKCFKKQMGLSPSEYRLKNK"
     gene            9986..12154
                     /locus_tag="PLI37_01890"
     CDS             9986..12154
                     /locus_tag="PLI37_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57802.1"
                     /translation="MKFYRTLTLISGLFFITTGLFANNGLIGWATCNTLGQNGTTGGG
                     RGQIVRVNTKAALEQYAKASGDYIILVEGSFTGTGVLQVSSNKTIVGTGNGATFDGFG
                     IDVKDKQNIILRNLTIKNAKPDAIAMRNTHHVWVDHCDLSNSDDGLFDFTIGSDYLTV
                     SWTLFHDHDKVALANSGTQHFEDVGKNKVCYHHNWFNNTVQRNPRIGYGMGHVFNNYY
                     SNVSSYCVGYHTGASVLIENNYFYKSKSPLNQMYTSVSTAANYADAEERGNIFDSTSG
                     NKKGTGKSFDPAQYYNYAFMLDDAADVPAIVQSKAGNVYGIEYEYLPSPANGAIDVRN
                     IGELWWSGIENVQSWEVYFGTDPDSMVKTSMTGRSFRPEELLPDTEYFWKVNAIKADT
                     TIEGSLWRFHTAPAKASKPYPANGELHAKLRQANTETTTIPTVLSWIPAFEAISYKVY
                     LDTTPDFSDSSYQASPATASIAPGQLRFGVKYYWRVDAVINNNTIIEGDTWDFISDVY
                     YSQEGITECEHMVLNGRAFKEEQSGVWFKASNNWVVSGEAGPGTMSSVWGGPDAICKV
                     SIDYFDESDGNGWYGFYVNDTYVRGWAASRNNDQMVTVNIENVRILRENELRIAFYTH
                     AGELCRTDKMNILITENLEEPQQLQPVVSTPYPPNQEMSMNIYTVSGLLLKTITVRSD
                     ANGRLSDQHWIELPLPSGLYFYTVQGENIPTRGAQRFFKP"
     gene            12214..15051
                     /locus_tag="PLI37_01895"
     CDS             12214..15051
                     /locus_tag="PLI37_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HOQ57803.1"
                     /translation="MKKICLLFSFCALFIYAGAQSPVLKIDVNVDEARKESEVNEPGY
                     TPWKIGKGIMSTSATFEGVQFTLSCVETTGGQLRGSWAKVMVQSPYFARLTCDGANID
                     YGSQTIEAGGKLLLEIAGLPVGTHTIQTMHNSWEDPARKAPSPMNVYLDSVLIHDSVS
                     VTSRAPVASEATILLTVLQVTAEDQVVRMLFEAVTSFTPPAGVNPQYNVWLNAIELNT
                     VEMSKLAKNPCPVDADMHVDADSGSLILSWSPASAKVEKHYLFLGTDKELIENADTTD
                     AALLAGIKACDDTTFFLADPYSMNTYYWRVDELDQDGILSKGNVWSFRPRQLAFRGAE
                     GYGRFATGGRGGKVVYVTNLNASGPGSFHEAVTDKSGPKTVLFAVSGRIELGGRIFMN
                     DYLTVAGQTAPGKGICLSRAPVGSGHEAITRFIRVRLGGGETADGMGMAGSNFSIIDH
                     CSISWAIDEVFSSRNAKNITLQRSMVTEALNVAGHKNYPEGKAHGFAGTVSGDIGTIH
                     HNLLAHNNGRNWSLGGGLDGAGYYAGRMDIFNNVVYNWDGRTTDGGVHEANFVNNYYK
                     RGPAGGTTTIFTAQLEGLGKGSQSYYYAGNVLENNNGSFLCDGTDNNCGRALQVASTQ
                     VVDWTVFVDEPFFPSFATVETAKDAYKSTLSDVGCTMPVFDAQDQRVVRETLNGTYTY
                     VGSKTGKKGLIDNEADAGGFEDYGNEVIDLDVFDTDRDGLPNWWEELHGSNPYSAEGD
                     FSDANADPDRDGYTALEDYLEWMSVPRYYLPKNKSIDIDLAPFAIAYTKNPQFSLATA
                     TDLSLNFSGSTVNVAPQGDYTGIRYFDFTVTDADNSSMTRRIGVYVQEDTASALSATQ
                     HKEMEFKVYPSTFSSELNIETQAGESKEINLILRNLNGQEMLREHYSIHAGAVRLQIK
                     CPAELPAQMYLLELNDAQTGMKYDVKRVFKK"
     gene            15137..19090
                     /locus_tag="PLI37_01900"
     CDS             15137..19090
                     /locus_tag="PLI37_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOQ57804.1"
                     /translation="MKKIFLSLSLLFSTFVLFAQTPAFPTAEGYGMWATGGRGGQVVE
                     VTTLEDNPSNPVVGSLRWAMKQHSGQPITIVFRVSGIIDLKGNDLRMKRNNVTIAGQT
                     APGDGICIKGGCVNLGGSRNLIIRHIRSRVGVLNDTVYNPEVAPTSADFIPGASLNIE
                     NGGNFIVDHCSFAWSAEENVGFYDNDNTTVQWCIFAEGLYDAGHGKGARSYGAVLGGK
                     TASYHHNLLAHNYNRSPRFGATTKNDVVMLLDFVNNVNYNFGKKNACYGGDNRQGSAG
                     LFQLNFVNNYYKQGPAYPNNRTAVFIGASYSTETGRSYGKWHLSGNYIEGSTNAALNA
                     DNYRGLDISAYTEAVPSITMATMKSDHIPVPYPIETESAHEAYSSVLRGVGAFPRDTV
                     DRRIIHEVTTCTASAHGTFNNHRVCGIINKPSDAGGYPVYNTYNQVQDEDHDGMADDW
                     ELANGLDPSNPDDRNLILKSGYTALDAYLCSLVGEDIPVETAKPYDIVVAKDGSGDFT
                     SINAAIETVPENAGRKTIFVKKGVYEEKVFIGNRWQDSRKVISLIGENVDSVVIVWED
                     YHGKTITYPGREGTIVADGMTAATFTVTAPDFYMENITVKNPSKAAQAEAIYQSGDRQ
                     VLKNCKILGNQDTHRTKKGRRYFYYQCTIEGGVDFIYAGGTCYFYQCQIVSNRNGYIT
                     APEDIVYTARLSNGKILYYGFIFNDCDLLAKDGANDVYLGRPWSSESGSIFMNCRLGS
                     HINRAGWNAWGGNEQNCSFAEYKSLNADGSALADVSGRVSWSMQLSDKDRYNLLGMNT
                     IYRAVSSSAFDPVSQFLPFSGPKSLTVSNRKLSWTAVENAVGYVVYANDAAIDFAETN
                     SYYDLIDRGTVNYKVKAIAGNGRLSTFNGLAGGVTANELDSLLNPKHWVNISTSVAPD
                     SAGVILQEPSAKQHLAGTQITLSAQRRYGYTFIKWINANNEVLSEEESFVLTLDKDEQ
                     VTAVFEKLPLYSLNCSVIGGPQELIQLCGSDTVIDGVTYYEAGTEVTLSVVDHPLFRL
                     DSWEDASTDSVRIITMNENKNLTARFRLHPFIVAWTFRMPGNSSYSSDYCSDENNIGE
                     FNLRDQQGNTADWLNYSLLEGGYLGRSAVLNGNALNDRYYYEASFSTKGHSDIVLRSS
                     MAVDSNGYSVQKAEYSTDNGASFETLGVIDIDSTKSWKNSVLPLPENAWDQDLVHIRW
                     IPDYTSEIHGDPQGTDGTAITDIYVLLQEKDALPKVSGRIPLKVWTDGNKLVYLQSGQ
                     PVRVVNVFDMTGRCVVRLSGEEIRSVNLSALSRGMYVLAAYNEINEMAVAKIILR"
     gene            19158..20447
                     /locus_tag="PLI37_01905"
     CDS             19158..20447
                     /locus_tag="PLI37_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOQ57805.1"
                     /translation="MRSFLFTIVVSFILSLPVCSQPLAFPGAEGYGAQTLGGRGGAVL
                     FVSNLNDSGPGSLRQAVEQEGPRTVIFNVSGTIELESDLTIGNPYLTIAGQTAPGDGI
                     TLKNYKIKLAADHVILRYLRVRRGNLSEKQDDAITIQNAENVIVDHCSFSWGTDEVVN
                     TWHGSKNLSLQWCIIAEGLHHKEHGFAATLGGINTSYHHNLIANCPGRNPSVGGNHNY
                     QTHRMDFRNNVIFNFGHRTFDGKPSSINIVNNYFKPGPNSSIDVFARIDEAGVYKEIP
                     ESSWYIEGNVWEGNESISRDNSLGVKGATQWMVDQPNEFSAITTEQAEAAYLSVLAGA
                     GACFPKRDGVDLRILEEARTGNTTYNKGVVMSTSEVGGWPVLNSLPAPADSDRDGMPD
                     EWETKHGLDPSNPEDRNTTNAEGYTMLEQYLNSLVQN"
     gene            20528..>21846
                     /locus_tag="PLI37_01910"
     CDS             20528..>21846
                     /locus_tag="PLI37_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOQ57806.1"
                     /translation="MKKIIILVCLCMAFLSANAQQKKIWNESPKEKKERLAWWTNDRF
                     GLFIHWGPYSLAARHEWVKKREQMDDKSYQKYVDHFNPDLYQPREWAKLAKTAGMKYA
                     VLTSKHHDGFAMFDTKFSDYKITNTLYGKDALKEWVEAFREEGLKIGFYYSLLDWHHP
                     EYTIDVKHPQSPENKEDYERLNRGRDMSVYREFLKNQVTELLTNYGKIDLLFLDFSFP
                     EEEFGKGCEDWGSVELMQLVRKLQPWIIVNDRMDLSDYWGGWDFATPEQHKVDKWPTY
                     KGEKIPWETCQTFSGSWGYYRDELTWKDNKQLLVLLIESVSKGGNLLLNVGPTGRGTI
                     DYRAENALLKIGDWMKYNARSIYGCTQAPDSFETPENTLLTYNPTTNRLYVHLLDYPL
                     RNITLKGMKGKVNYLQFLHDASEIKISTSKGFIAIDQGSTEDNDLRL"
     assembly_gap    21847..21946
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21947..23602)
                     /locus_tag="PLI37_01915"
     CDS             complement(<21947..23602)
                     /locus_tag="PLI37_01915"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOQ57807.1"
                     /translation="MKLLFQSMGILSVLIICTTGCSPTKSKQWAHLRQDTLYIKASQF
                     RAGENTIALGDSANLIHFVIAEKDRVPFNQSVVKQTNEQAAYGPDAKLPYFTVRFAVP
                     IPPAYTPFEVGTLAGLEHGVYHHNHSPGFEIMPNGDALAVFFSSPAGKSESDTAATFV
                     QARLRHGSEDWDMPEVFFDTQFGNDQSGLLWNDMGKIWFFGGGRYISDYVPFRIAVST
                     DNGASWTFSVPQLDTAATDYTAQPVVNAFRDPQGGIYMVCDGDGSESFLWYSADEGKS
                     WKDKGGRTGSRHSTILPLDDKGTLLAQGGKNADIEGWNVRNISHDWGVSWEEGTSSPF
                     PPLGTAQRPSMIRLASGCLFYVTDSYMHKKNIAPPAGWEFGNNAVVAISKDNGETWHI
                     KPLPVQLPQRHRPPHGSLGYSTARQAPNGVIHILTTANFPPIHYELNEAWIWSDLADI
                     TPESDGGEVKSFTETYKDGRLKSAWSARICPNGRYLLHGETTDYYENGAIQHVAVYEN
                     GRKSGEELYYDKEGKLEWKWQRDLLTNTGVWTQYWPNGNKKVES"
     gene            complement(23642..24934)
                     /locus_tag="PLI37_01920"
     CDS             complement(23642..24934)
                     /locus_tag="PLI37_01920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ57808.1"
                     /translation="MSGASNKDLVELKENHSHPEKITKEQWKASALAGMASYLDAGSI
                     VALGAGLALFQEYLNLSNSAVGLLAAIGPNAIGCAIGSIIGGRLGDKWGRKRIYQIDL
                     LVYAFGVLLITFCFNATMLFVGTFIVGVAVGADVPTSLAFVGEVAPDKARGKLLGFTQ
                     VAWSFGPVVVLWLAMFLADLGVTGIRIVFLHLFVVALVTWYLRRSLSESARWTKAAKT
                     KGSQLRLLLKGSNLRGMAYTATIYLFWNLAAGTAGIFNPYIIKTLNAGSSQALSVGLS
                     SAGFIISIIVTSLVFMRYSDRNFKTRKLLWGIGAVMQVVAYSLFLFLPFTIPVIIANI
                     LMFSSGGALAGEAFYKVFSQELFPTMLRGTAQGITFGISRTILGVWSFFVPILAKTGI
                     GPVAALLSVFLLISGSVGFFFMPDTSGKSLEQIAKERA"
     gene            complement(24960..27662)
                     /locus_tag="PLI37_01925"
     CDS             complement(24960..27662)
                     /locus_tag="PLI37_01925"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOQ57809.1"
                     /translation="MNVRNLRTEYKINPLGIANLNPRLSWRLDTEKENSLQTAYRIKV
                     ASTPEALKRNQLVWDSGVVSSDRSVFVEYQGPQSVSGQRLYWQVTVWDNQGNQATSGE
                     TAYWEMGLLTSGEWKAAWITPGWEEVTSQSNPCPFFRKEFDVKKRVAKARMYASALGL
                     YELYLNGEKLGKDYFNPGWTTYSKRIQYQVYDLTALLKQGRNALGAIVGDGWYRGFFG
                     FQGERNLYGDKAGLITQLEITYEDGSKEWILSDPSWKSGKGPLLKSEIYHGEFYDARL
                     EIPGWAEPGFDDSKWEKVTILNQSNEVLVPTEGEPVRITETLKPVKKFVTPRGELVFD
                     FGQNLVGWVRFRLKGERGQTIVLHHAEVLDQEGNFYTDNLRVAKAEDVYIFKGEGIEE
                     YEPRFTFHGFRYLRVSEYPGEISLDDVEGRVVHSDMEKIGEFECSDPLINRLQQNIQW
                     GLRGNFLDVPTDCPQRDERMGWTGDAQAFAPTACFNMNTGAFFTKWMKDLQLDQKDDG
                     NVPWVVPNVLEGGGGTGWSDGYGGTGWADAAVIVPWTVYQSYGDVQILENQYESMKAW
                     VEYMIRHSGSRYIFDYGFHFGDWLSFAEYYTYNYNAPDYGYAGAHTEKDLIATAYFYY
                     STSLMSKIASLLGRNEDSERYAGLLPKIKEAFHREFITPNGRLVSGTQTAYVLALGFD
                     LIPEEEREAMAKRLADDVRYFGHLTTGFLGTPLLCRVLSDNGYPDLAYQLLFNKRYPS
                     WLYPVTMGATTIWERWDGIKPDGSFQTVGMNSFNHYAYGAVGQWLYSTVAGLNIDSSK
                     PGYKHSIIRPCPTRRLNYAKASLQTMYGLLLSSWEIKGDDIIYKFRIPSNTTATIYLE
                     AEKISYSQKAQDLSGIKKMGDKQFVIEAGSGSWEFVAHKAIINN"
     gene            28081..>29510
                     /locus_tag="PLI37_01930"
     CDS             28081..>29510
                     /locus_tag="PLI37_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57810.1"
                     /translation="MKKILTPLLTTLLLFLVAVPKADAIITDPNDYIIYDSGIMADSA
                     HCSTWEFPPFMTITDNVSVKKVNKWGRHNNSVVDYGYPPKTDVATLKGKVDLWGIVIG
                     QEKGYSGISYTAFLEQPEEERPLFYVNRWKGGWLYGRPTDSIVIRIPTTSDSIFIQGC
                     GTNSFYAIMAYELHRGKDSAWYIGGNWGNVFNLGFKPKHAPGTDSTDIVVLGPHKDWY
                     TTGEEWQGNAFQMRLVNAADQNEDGTMKNKKSERWGFFEGTSINRIKIFGQIEKGSVP
                     EFDGTIAGWDFLYRPKASGTVDLTSFVTDIDGEWEADYYSLYASDLGVVRGMLTASSP
                     SDGMQLGKDCGVKAVMISPAGKNDTVDFTLAEEHANYFQIDVSLQDFQEINLNFDFNL
                     RDAADSCVVAYAVDGADTFTVAGKFAAPEDWDGLTHVSVDLPELTNQSYVTIRILQNN
                     GALDENGQFILANLSINGFDYVEHKEG"
BASE COUNT         8009 a         7327 c         6773 g         7101 t
ORIGIN      
        1 ggcaccgatt accgaaatca gtactgctcc gacaatcacc aacacatcta ataaggaatt
       61 gtccatagtg cccgaaattt gttgtccaaa agtagtgttt tcccggggaa ttacaaaaag
      121 atgtaaacct gttttggtcg attttgttaa tttatgtacc tttgcgccgt ttttccaata
      181 tagtatcatg tctcttaatt tcattactgc tcaggaagca gcttctttca tcaatcacaa
      241 cgacaatata ggatttagtg gttttactcc tgccggagca cctaaagtca ttgcacaagc
      301 catagccgaa aaatcgcaag ccgaacggca agccggacgg gaatttaaag tcggtgtgtt
      361 tacaggagct tcaaccggtg attcgctcga cggtgtgctc gcccgcgcag aggctgtgaa
      421 atttcgcaca ccttatcaat ccaatccgga tttacggagt ggtatcaaca aggggaacat
      481 tccctatttt gatcttcacc tctcgcaatt ggctcaggaa ctacgctacg gatattacgg
      541 aaaaataaac acagtcataa tcgaagctgc cggcgttaac gaaaaaggcg aaattctact
      601 taccaccgga gtgggtatca ctcccacggc agcccgcatg gccgacaaaa tcattgttga
      661 gctcaacagc cggcaccctt cgtttatcaa cggtttgcac gatctttacg aaccggccga
      721 tcctccccat cgtcgcgaaa ttcccattta caaacccagc gaccgcattg gacaaactct
      781 cttgaaagta gatccctcca aaatcgttgg tgtagtcaaa actcaattac ccgacgaagt
      841 taaggcattc tccgaaccgg acgaaaccac caaacgcatc ggagatcgcg tagccggttt
      901 tctggtcagc gaaatgagag caggccgtat tcctgcttcc tttcttcctt tgcaatcggg
      961 cgtaggcaat attgccaatg cggtattagg ttcgttgggc gccagccccg aaattcccgc
     1021 tttcgaaatg tttaccgaag ttatacagga ttccgtgatc gaaatgatgc aaagcggcga
     1081 tgtaaaattt gccagtggct gttcacttac catcacaagt tcggcccttg attccattta
     1141 cgagaactgg gactttttca agcatagact ggtcctccgg cctcaggaaa tttccaattc
     1201 tcccgaactg gctcgtcgca tggggctgat taccatcaac acggctctgg aggccgacct
     1261 tttcggcaac gtaaacagca cccacgtact gggcactaag atgatgaacg gtattggagg
     1321 ctcgggcgat tttacccgca attcctacct ttccatcttc acttgtccct cgactgccaa
     1381 agacggcaaa atcagcgccc ttgttcctat ggtttcgcat accgaccaca acgaacattc
     1441 ggtgaaaatt ttaataaccg agcagggagt tgccgactta agaggcaaat ctcccatgca
     1501 gaaagcgcaa accattatcg agaattgtgc tcatcccgat tacaaacaat tgctgacgga
     1561 ttaccttaaa caaaccaatg gcggaaatac tccccataat ctgggcaatt gctttaatat
     1621 gcaccgggaa tttctcgact cgggcgacat gcgcaacacc aagtggtaaa atgatccggt
     1681 tattcctggt tggttacatg ggtgccggca aaaccaagtt gggacaagcc attgcgaaag
     1741 aactgcaact ttcttttgtc gacttggatc aatacatcga agaacggcgc tttaagccca
     1801 taccggcact ttttgccgaa ttcggcgaag aaggttttcg caaaatcgaa caaggctatc
     1861 tgcgagaagt ggcccaattc gaaaatgtat tgattgccac cgggggaggt accgcttgtt
     1921 tcgaaaacaa catggatttt atgaactctg ccggcgacac cgtctatctt cagctcaatg
     1981 ccgaagaatt gttcaaaagg ctgcatcgaa aaaagcaaaa cagacccctg ctcaacaccg
     2041 tcagcgacga cgatttgctc actaaaatca gagaaatgct ctctcaaagg gaaagttttt
     2101 acaataaagc caaatacatt ctgcctaccg gaagtctcaa caaagaagaa tccctgacac
     2161 tttttaaaca aatttattca aaacccccaa gccccacgat ctaggctgcg gtattgtatg
     2221 gcttcggcaa tgtcacgggt acgcaagcgt tccgaagcgt ccagatctgc aatggtgcgg
     2281 gctactttaa ggatacggct gtaagctctg gcagaaagat ccagtttttc catggcattt
     2341 ctcagcaaaa cataagcatc gggctccgcc tgagcatagc gttgcacctg ctttaagctc
     2401 atttgggcat tacaaaataa tcccttatcc ggtttaaagc gatcgttttg caagcgcctg
     2461 gcttttatca ctctttctct gatactttgg ctgctttcct gctccctgac gctacttagt
     2521 tgctcgtatt ccaaagggac aatttccatc tgaatgtcga tgcgatccat caagggcccc
     2581 gaaatgcgat gtatgtattt atgcacttgg gaggcggtgc aaacacattc ctttgtggga
     2641 tgattgaaat atccgcaggg acagggattc atcgatgcca ccagcataaa tcccgccgga
     2701 tagcggacag cgtagcggga acgggcaatg ctgatgctgc ggtcttccag cggttgccgc
     2761 aacacttcga gtacagagcg ctgaaattcg ggcaattcat ctaaaaacaa aactccgttg
     2821 tgggccaacg aaatctcccc gggttgagga ttgctgcctc ctcctatcat tgccaccgaa
     2881 cttatgctat gatgaggcga acgaaaagga cgactcgtca acaacgaaca acgggcatct
     2941 atcttgccgg ccaccgaatg aattttggtg gtttccaaag cctcgtccag gctcatgggc
     3001 ggcaggatac caggtatagc cttggccatc atggtcttac cggaacccgg aggccctatc
     3061 atcaccaaat tatgacctcc ggcacaagcc acttcaatgg ctcttttcgc ccgttcctgc
     3121 ccctttattt cggcaaaatc cacttcggga accacctggg ccccgtcaaa gaaatccaaa
     3181 cgactacaat aaggctcggg gcggctttcg cctttcaaaa aagcgaccac ttcactcagg
     3241 tgcttcatac cgtacacctc caatccttcc accacggaag cttcggcagc atttttatgc
     3301 ggcagtatca gacgtttaag gcccatctct ctggcagtca aagcaatggg caaaacacct
     3361 cttacctctt gcagacaagc atccagtgaa agctctccga taaacaggca atctttgagc
     3421 gactccgccg gcacatattc tccggcagcc atcaggccta cactcagagg aagatcgtag
     3481 ccggaacctt ctttgcgcaa atcaccggga gccatgttaa taactacttg cttacgggga
     3541 atatccattc cgcagtattg caaagcggag cttatacgtt cgtggctttc gcgtacggcg
     3601 ttgtcgggca aacccaccag gctgaaacgt attccccgcg agacatgcac ctctacgtaa
     3661 accggaatag cccctattcc ctggatagcc gcagaaatca atttcacata catgattata
     3721 attttcctgc aaatatacat agatttaata ttatatttac acaacaagaa gaaatgttgt
     3781 ccggaaggtc tcacctgtgg aggccaatta ctgtcttctt accggactga acagcggcaa
     3841 caaaaagatg attcccacag tagcaggccc tattagcatt ttatcgagca tgaaaaactc
     3901 agccgttttt tataaaggaa aatgaatata atgcttattt ttgtgcaagt ttgtttaata
     3961 cacattgaat taatatcgcc atgaaaaaat tacctctgtt tatttttgtt ctctttactt
     4021 gctctgtttt caatttatta gcccaactcc cgtacaccaa tcctgccttg tcgcacgagg
     4081 ctcgtgccca agaccttctc ggccggctca ctttggaaga aaaagtggcc ctcatgcaag
     4141 accaatcgcc ggcggtgccc agattgggtg tgcccgagtt cggttggtgg aacgaagcct
     4201 tgcatggggt ggcacgaagc ggttatgcca ccgtttttcc ccaagccata ggcatggctg
     4261 ctacttttga cgaagacgcc gtctttagga cctttaccgc cgtcagcgat gaggccaggg
     4321 ctaaagccac ccacagcaag caggtggaag gccggcgggg aaagtacaaa aacctgacct
     4381 tttggacgcc caacatcaat attttcagag atcctcgctg gggacggggc caggaaacct
     4441 atggcgaaga tccctatctg accacccgac tcggcgtagc cgtagtgaaa ggtctgcaag
     4501 ggcctgacga cagtcattac gccaaggcta tggcctgcgc caaacactac gccgtgcaca
     4561 gcggtcccga atggaaccgg cacagcttcg acgccaaaaa cattcgcccc gaggacctgt
     4621 gggaaaccta tctgcctgct ttcaaagcct tggtgcaaga ggccaacgtc aaacaaataa
     4681 tgtgcgcata caaccgctac gagggagatc cttgttgcgc aagcaacagc ttgttggtgg
     4741 acatcctcag agagggctgg aattaccggc acgtggtggt aagcgattgt ggtgccatca
     4801 atgatttcta caacaggggc aggcacgaaa cccatcccga ttcggcaaca gcctccggcg
     4861 ctgccgtccg ggcaggcaca gacctggaat gtggacgcag ctacaggtct ttagttggag
     4921 ccgttaacaa agggctgatt gccgagtccg aacttgatgt cagcctgaaa cgtctgctca
     4981 aggccaggtt tgaactaggc gatttcgatc cggatagcat agtgccctgg acgcgcatcc
     5041 ccatgagcgt cgtcaactgc gaagcccatc aacaactggc tttggaaatg gccagaaaat
     5101 ccctggtgct gcttaaaaac gacaagaaca ctcttccttt gcccaaaaaa gccggcggga
     5161 ttctggtgat ggggcccaac gccgataacg aaagcatgca atggggcaat tacaacggca
     5221 cgccctacca caccatcacc atattgcaag gtttggaagc cgcccttggt gcatccctgc
     5281 cctgcgtgcg gacaccccac gtcgtaaccg aagacaaagt gcagttcacc gtcgattccc
     5341 tgatgaaaga cctgcctgct tgtgataagg tgctctttgt aggaggcatc tcaccggcac
     5401 tcgaagggga acaaatgcgg gtaagtctgc ccggattcag gggtggtgat cgcactacca
     5461 tcgaattgcc ggccgttcaa cgagctctgg tcaaggcgct ggcagaggca ggcaaacaaa
     5521 tcgtattcat aaattgctcg gggtcagccg tggggctctc ggaagaagcc aagatttgcg
     5581 acgccatcct gcaagcctgg tatccgggcg aaaagggggg acaagccgtc gctgaggtgt
     5641 tgtttggaga ctacaatccg tcaggccgtt tacctgttac gttctatgcc ggcgaccatc
     5701 agttgcccga tttcgaggat tacagcatgg aaggaagaac gtatcgcttt ctggaagaaa
     5761 aaccccttta ccccttcggt cacggattga gttacaccag cttcgcctac agcaagccca
     5821 aaatcaaaaa ggggacaaaa accttgtccg taacagtgac caatacaggg aaacgcagtg
     5881 gtgaagaagt aatacaactt tacgtgcaac gcaccgctcc ccaagcgaga ggcaaagccc
     5941 tcagaggctt ccggcgcatc aatctcggac cgggcgaaag caaggaagtt agcttttctt
     6001 taagtggagg caccctggac ttttacgact ctaaccaagc catagtgacc accttgcccg
     6061 gcacttatct gttggaagta ggaggcagtt cagatcctgc agcctgcgta cggaccagtt
     6121 atacactttg agtctaaagt tgagagcttg atgaaactat acctgagcat cctgtttttc
     6181 ttgttttcgg ccgcaatgct gtttgccggg ccctcctgcg atcttaaata ttacacaggg
     6241 gccgacggct tcaggaacag ttccgccatt gacatactac aggccaaaaa cggtttaatt
     6301 tggatcagca cccggaacgg gctcaccttg ttcgacggct atagtttcan nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tagtctttta cggaccggat aaaggcatta
     6481 ggggagaaaa cattcattgg attgcccagg acagagaggg ggacgaatgg gttcttacca
     6541 accggggtgt gagcgttatc ggtaaaaaaa cactgaccag ccacgtggaa tttcactatt
     6601 tcctcgaaaa gggcgattac atctggctgg catccaacga cgggaaactg gcgagatact
     6661 ctttcgagca ggatagtctg gaattcatca atcttccgag ttcaaccaca tcctttcatt
     6721 ttatgggagc gatgaaggga gataccatcg cgctgggtac aaacaaagga ctgctcttaa
     6781 tcaactcatg cgacaaaaca cacaagcata taccggttga tttgattccg gccggaaatg
     6841 ccggtatctc atttgtttat caggatgctt ataacgatta ttggctgttt gccgaaaacg
     6901 gggtttaccg ttacgaacag gttacatcgg aaattcgttt ttttcaatcg cccgatgtat
     6961 atcttcaaca cgagtaccca aacaagagct ttgcttacga agacccctac gggcatttgt
     7021 ggctgattcc ggggaatggc aatttttgct atttcgaccg tgaacagcaa gaattacgct
     7081 accattactc cgaaccggga gatgcttcta ctctgatttc tccttccgtc aggcattttt
     7141 tgttcgaccg gcaaggaaat atctggattg ggaccaacaa cacttttggc aagctttcct
     7201 tttatgaaag cacctaccgg gtaatcccgg ctgaaagcga tgaatttgaa gttcgcgctt
     7261 tattcaaaga cagtcgagaa gtcctgtggg tagcctctaa acatcataaa ttaaagctat
     7321 tggacgagaa ttatgctaaa attgactatc gcctttacga tgccaacacc catagagaac
     7381 tccggattga ctttaatatc tattccatat acgaagataa ggcgggtaac atctggctgg
     7441 ggtccagatc aagaggtttg tacctcctga aaagggatga gcaaaacccg tatgcttttg
     7501 ccgtgcaaca ttttatccat gatcccgcag acagcatgag cttaagcgac aacagcatct
     7561 actcgatatt tcaggacagt cgcaaacgaa tatggctggg cacctacgga ggcgggctta
     7621 atttgatccg ggaagacgca tcgggaaaaa tcaatttcat tcacagcggg aacagtttaa
     7681 aaaactatcc cgaagtgtac gctcagatag tacgttatat taccgaagta gacaacaccc
     7741 tgcttgtggg cagtatgcac ggtttattca gtttctcctc agcatttgaa tcgcccgaag
     7801 aaattcgctt ttcccgaaac gcacgcaatg aatatatagc cggctccctg agcaataacg
     7861 atgtaatgta catttattcc gattccgaaa atcaagttta tatactgaca caaaacggcg
     7921 gagtgaataa attggtctcc ggcaatctac tctccgacaa catggtattc gaagtaatcg
     7981 gcgaacggga aggattagcc tccgatttga ccctctccat gaccgaagac ccatcgggca
     8041 agctttggat tgcctccaaa caggatttat cgagatacaa tccctccggc ggtttgatca
     8101 acaatttcgg cagctattat tttaaacagg attttgtttt ttctgaatcc aaagtcatca
     8161 acacctccaa aggcagtatt cttttcggaa ccaacaaagg gatactggaa attaatccct
     8221 ccactatgtc cggaagcaat tatgtgcctc ctctggtttt taccgaaata aaactcaata
     8281 atcaagccgc ctctgttcca attgatcaac tgaaagacat tgatctgaag tctaatcaac
     8341 gaaatttttc catacaattt tccgctttgg atttcaacaa ttcaaaagag atcaggtatg
     8401 cctatatact cgaaggcctc gaaaaagaat ggcattatgt gaacgacaac cgctcggccg
     8461 aatacatcaa gattcccaaa ggacgctatc aattcgtggt gaaatcgacc aacagcgacg
     8521 gggtgtgggt aaacaatgaa cgaaagttgg atattaccgt tttgcccact ttttgggaaa
     8581 ctccctgggc ctggctcctt tatattttct tctttatact gttggtgagc agcattgtat
     8641 atgtgttgtt tattatttac cgcctgaggc acgaagttac cgccgaacag caattgtccg
     8701 aaataaaatt gcgcttcttc acggatattt ctcacgaact cagaactccg ctcaccctga
     8761 tttccagccc tgtaacggat gttcttgaac acgaaaaatt atcggagact gccagagaaa
     8821 atttaacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacg caaaaatcga
     8941 agttctcagc gaagtaggga aaggcagcag cttcaggttg ttttttccct tgggaagcga
     9001 acattttgca aataatcctc aggtggaatt tcttttatcc gatcgacaat cggatgtgaa
     9061 cttaccgtcc gaagaacaaa gccaaacagg gcaagaagac gatgtgcagg ataggcaatc
     9121 catattgatt gttgaagaca atcccgaatt aatcagcttt ttgcacaata ttctatcgag
     9181 agattacacg gtgctgcagg ccactaacgg gctggaaggg tggaataaaa cgcaggaaat
     9241 gctcccggac ataattatca gtgatgtggt aatgcctgta atggatgggc tggatatggt
     9301 aaagaaaata aaagagaaca acaatacctg ccacattccc atcatactat tatcctcaaa
     9361 agcctccctg gacgacagaa tcaaagccct cgagagaggc atcgacgatt atatcactaa
     9421 gccattcagc gccagccacc ttataactcg cgtacagact ttgcttgaac aacgcaagtt
     9481 tttacaagac tattacctga acaaattcag caaaaatcga acaagcgatg cggatttaag
     9541 ttctgagtct cccgctattg cctcctacga tgaacaattt atgaaacagg tcatcgatta
     9601 tattgagcaa aacatcgata atgttttgtt ggtggtggaa gatcttgcta aggaatttcc
     9661 catgagccgg tctgtttttt acaaaaaaat caaaagcttg tttgggcttt cacctgtgga
     9721 tttaattcgt actatccgta ttaaaagggc tgtacaatta atagactcct ctactcattc
     9781 atttactgaa atagcctata tgtgcggctt caacgatctc agctattttg gaaaatgctt
     9841 caagaaacaa atggggcttt caccctcgga gtataggtta aaaaacaaat agttccaatc
     9901 tggaaacaat ttttcctaat tcggccaata actattggct aaacttgcaa gttgaatgtt
     9961 atttcgtttt ttattgaatt ttgttatgaa attttatcgt acccttacac ttatcagcgg
    10021 tttatttttt attacaaccg gccttttcgc caacaacggt ttgattggct gggccacctg
    10081 caacacctta ggccaaaacg gtaccaccgg cggtggtaga ggacagattg tgcgggtaaa
    10141 caccaaagca gctctcgagc aatacgcaaa agcctcgggc gattatatta ttttggtcga
    10201 aggatccttc accggtaccg gtgtactcca ggtaagcagc aataaaacca tcgtcggtac
    10261 gggcaatggg gccacctttg acggattcgg cattgatgta aaagacaaac aaaacatcat
    10321 tctccgcaat cttacgatca aaaatgcaaa acccgacgcc attgccatgc gcaatacgca
    10381 ccacgtctgg gtagatcatt gcgatctatc caattccgac gatggcttgt ttgatttcac
    10441 catcggctca gattacctga ccgtttcctg gactttgttt cacgaccacg acaaagtggc
    10501 tctggcaaat tccggcaccc agcatttcga agacgtcggc aagaataaag tatgttatca
    10561 ccacaactgg ttcaacaata ctgttcagag aaatccccgc ataggctacg gcatggggca
    10621 tgtattcaac aattattatt ccaacgtaag ttcctattgc gtgggttatc ataccggtgc
    10681 cagcgtattg atagaaaaca actattttta taaaagtaaa tcgcccctga atcaaatgta
    10741 tacgagcgtc tctacggctg ctaactatgc cgatgccgag gagagaggca atatcttcga
    10801 ttctacttcg ggcaacaaaa aaggcacagg aaaatccttt gatcccgcac aatactacaa
    10861 ctatgccttt atgctggacg atgccgccga cgtgcctgct attgtacaaa gtaaagccgg
    10921 gaacgtctat ggcatcgagt atgaatacct tccctccccg gcaaacggag ccatagatgt
    10981 aaggaacata ggtgaattat ggtggtcagg tattgaaaat gttcaatcct gggaagttta
    11041 ctttggaacg gatcctgact cgatggttaa gacttcgatg accggacgct cttttcgtcc
    11101 ggaggagttg ctgcccgata cggaatattt ctggaaagtg aacgccatca aggccgacac
    11161 cacaatcgaa ggaagtttat ggagattcca tacagcaccc gccaaggcat ccaaacccta
    11221 tccggccaac ggagaacttc atgccaaact tcgccaggcc aatacggaaa caaccaccat
    11281 tccgacggtt ttgagctgga ttcccgcctt tgaggcaata tcgtacaaag tttatcttga
    11341 taccactccc gacttttccg actcgtccta tcaggcaagt ccggccactg cttccattgc
    11401 tcccggacaa ctcaggtttg gcgtcaaata ttattggcgt gtggatgcgg taatcaacaa
    11461 caacactatt atcgaaggcg acacctggga ctttatttcc gatgtatatt attctcagga
    11521 aggcatcacc gagtgtgagc atatggtatt gaacggcagg gctttcaaag aagaacaatc
    11581 gggggtttgg ttcaaagcct ccaacaattg ggtggtgagt ggagaagccg ggccgggcac
    11641 tatgagcagt gtttggggtg gaccggacgc tatctgtaag gtttccatcg attatttcga
    11701 cgagagtgac ggcaacggct ggtacggttt ttacgtgaac gacacatatg taaggggatg
    11761 ggccgcctca agaaacaacg accaaatggt aacagttaac atagaaaacg tacgcattct
    11821 cagagagaat gaactgagaa ttgcctttta tacccatgcc ggagaactct gccgtaccga
    11881 caagatgaat atcctaatca ccgaaaatct ggaagagcca cagcagttgc agcccgtcgt
    11941 gagcacgcct taccctccga atcaggaaat gagtatgaac atttatacgg tttcgggctt
    12001 gttgcttaaa accattaccg tgcggtccga cgccaatggc aggctctccg atcaacattg
    12061 gatagaattg cccttgcctt cgggtctcta tttctatacc gttcaggggg aaaatatccc
    12121 cacgagagga gcacaacgat tttttaaacc ctaacaggta tccgcatcaa tcccggcctt
    12181 aaatctatat catacccttt taatccggtc aaaatgaaaa agatctgcct gttattcagc
    12241 ttttgcgccc tgtttattta tgccggagct caaagccccg tccttaaaat cgatgtcaac
    12301 gtggacgaag cccgcaaaga atccgaagtg aacgaaccgg gctatacccc ctggaaaata
    12361 ggtaaaggta ttatgagcac atcagctacc ttcgaagggg tacaatttac tctttcctgt
    12421 gtggaaacga ccgggggaca attgagaggc agttgggcca aagtcatggt acaaagcccg
    12481 tactttgccc gacttacctg cgacggagcc aatatcgact acggctcgca aaccatagag
    12541 gccggaggca aactcttgct cgagattgcc ggactgcctg tgggaacaca taccattcag
    12601 accatgcaca attcatggga agatccggca agaaaagccc cttcacccat gaacgtttac
    12661 ctggacagcg tgctgataca cgattcggtc agcgttacca gccgggcgcc ggtcgcttcg
    12721 gaagccacca tcttactgac agtgcttcaa gtgactgccg aagatcaagt ggtacgtatg
    12781 ttgttcgaag ccgtgacatc ctttacccct cccgcaggag taaatcccca atacaatgta
    12841 tggctcaacg ccatcgaact caatacggta gagatgagca aactggctaa aaacccctgc
    12901 cccgtcgatg ccgatatgca cgtggacgcc gacagcgggt ctctgatatt aagctggtcg
    12961 ccagcctcgg ccaaggtgga gaaacattac ctctttttag gtaccgacaa agaactgatc
    13021 gaaaacgccg acaccaccga tgccgccctg ttggcaggca tcaaagcctg tgacgacacc
    13081 accttttttc tggccgaccc ctacagcatg aacacctatt actggagggt ggacgaactg
    13141 gatcaggacg gcatcctgtc caaaggtaac gtctggagct tccgccccag gcaactggcc
    13201 ttccgcggag ccgaaggcta tgggcgtttt gccaccggag gacgaggcgg taaagtggtg
    13261 tatgtgacca atctaaacgc cagcggaccg ggcagcttcc acgaggcggt aaccgacaag
    13321 agtggcccca aaacagtgct gttcgccgtt tcaggcagaa tagagttggg cggccgcatc
    13381 tttatgaacg actatcttac cgtggccgga caaaccgctc cgggtaaagg catttgcctg
    13441 agccgggccc ccgtcggctc cggtcacgaa gctattaccc gttttattcg tgtccgactg
    13501 ggaggaggtg aaacggccga cggcatgggt atggccggga gcaacttcag cattatagat
    13561 cattgctcca taagctgggc catagacgaa gtgttcagct ctcgcaatgc caagaatatc
    13621 accctgcaac gcagcatggt aaccgaagcc ctgaacgtgg caggccataa aaattacccc
    13681 gaaggcaaag cccacggttt tgccggcacc gtcagcggcg acatcggcac tattcaccac
    13741 aacctgctgg cccacaacaa cggccgcaac tggagtttgg gcggaggcct ggacggcgcg
    13801 ggctattatg ccgggcgcat ggatattttc aataacgtag tgtacaattg ggatggtcgt
    13861 actaccgacg gtggagtaca cgaggccaat tttgtcaata attactacaa gagaggtccc
    13921 gccgggggta caaccaccat ctttaccgca caattggaag gtttgggtaa aggatctcaa
    13981 agttattatt atgcagggaa cgttcttgaa aacaacaacg gcagcttttt gtgcgacggt
    14041 acggacaata attgcggccg cgccctgcaa gttgccagca cccaggtggt agattggacc
    14101 gtatttgtag acgagccctt cttcccctct tttgccaccg tcgaaacagc caaagacgcc
    14161 tacaaaagca ctcttagcga tgtaggttgc actatgcctg tttttgacgc ccaggatcag
    14221 cgagtagttc gcgaaaccct gaacggcacc tatacctatg tgggcagcaa gaccggcaaa
    14281 aaaggcctga tcgacaacga agccgatgcc ggcggtttcg aagattacgg caacgaagtc
    14341 atcgatcttg atgtcttcga taccgaccgc gacggactgc ccaactggtg ggaagaacta
    14401 cacggcagca acccgtattc cgccgaaggc gatttctccg atgccaacgc cgaccccgac
    14461 agggacggct ataccgccct ggaagattac ctggaatgga tgtcggtacc ccgttattat
    14521 ttgcccaaaa acaagtcaat agatattgat ctggcaccct ttgccatcgc ttataccaag
    14581 aatccccaat tcagtttggc tacggctacc gatctgagtc ttaacttttc gggatccacc
    14641 gttaacgtgg cacctcaggg cgattatacc ggcatccgct atttcgactt tactgttacc
    14701 gacgccgaca acagcagtat gacacgccgc atcggtgttt acgtgcagga agacacggcc
    14761 tcggccctct ctgcaactca acacaaagag atggaattca aagtctatcc cagcactttc
    14821 agctctgaac tcaacatcga aactcaggcc ggcgaaagca aggaaatcaa cctgatttta
    14881 cgcaatctga acggacagga aatgctcaga gaacactatt ccatccatgc aggtgcagtc
    14941 cgcctccaaa tcaagtgccc ggccgaattg ccggcacaaa tgtatctgtt ggaactaaac
    15001 gacgcacaaa caggaatgaa gtacgatgtg aaaagagtat ttaaaaaata gaaccgctgc
    15061 gcttgccaac ttagcccaga ctaaagttcc attagaatca ccgatataga aactatctca
    15121 tttataaaat tcaagcatga aaaaaatctt tttatccctc agtttgcttt tttcgacttt
    15181 tgtgttgttt gctcagacac cggcttttcc cactgccgaa ggttatggaa tgtgggcgac
    15241 cggaggtcgg ggaggccaag tggtagaagt aaccacactc gaagacaatc cttccaatcc
    15301 tgtggtgggc agcctgcgct gggcaatgaa acaacactcg ggtcagccga taaccatcgt
    15361 tttcagggtc tcgggtatta tagaccttaa gggcaacgat ttgagaatga agagaaacaa
    15421 tgtaaccatc gcgggccaaa ccgctccggg agacggcatt tgcatcaaag gaggttgtgt
    15481 caacctggga ggcagccgga atttaattat acgccatatc cgttcccggg tgggggtact
    15541 caacgatacc gtttacaacc cggaagtcgc tcccacctct gccgatttta taccgggagc
    15601 cagcctgaac atcgagaacg gcggtaattt tattgtagac cactgctcct ttgcctggtc
    15661 tgccgaagaa aacgtcggct tttacgataa cgacaacacc acggtgcaat ggtgtatttt
    15721 tgccgaaggg ctttacgatg ccggtcacgg caagggagcc cgcagttatg gtgccgtgtt
    15781 gggaggcaaa acagccagct accaccacaa tctactggca cataattaca accgttctcc
    15841 gcgtttcggc gccaccacca aaaacgatgt ggtcatgttg ttggattttg tgaataacgt
    15901 aaattacaac ttcggcaaaa agaacgcctg ctacggcggc gacaaccgcc agggaagtgc
    15961 aggactgttt caattgaatt ttgtaaacaa ttactacaaa caagggccgg cctatcccaa
    16021 caacagaacg gctgttttta tcggtgcctc ttacagtacc gaaacaggta gaagttatgg
    16081 aaaatggcat ctttcgggca attacatcga gggttcgacc aatgcggcgc tgaatgcaga
    16141 caactataga ggattggata tcagcgctta tacagaggcc gttccaagta tcactatggc
    16201 caccatgaag tcggatcata tccccgtgcc ctatcctatc gaaacggaat cggctcatga
    16261 ggcttatagc agcgttttga gaggcgttgg ggctttcccc cgcgataccg tggacagacg
    16321 tattattcac gaagtcacca cctgcacggc ttctgcccac ggcaccttca acaatcaccg
    16381 ggtttgcggt attataaata aaccttccga tgccgggggt tatccggttt acaacaccta
    16441 caatcaggta caagacgagg accacgacgg tatggccgac gactgggaac tcgccaatgg
    16501 cctggatcct tcgaatcccg acgaccgcaa cctgattctg aaaagcggat atacggcttt
    16561 ggatgcctac ttgtgcagcc tggtcggcga agacattccc gtggaaacag ccaagcccta
    16621 tgatattgtt gtagccaaag acggcagcgg tgattttacc agcatcaatg cggctatcga
    16681 aaccgttccc gaaaacgccg gccgcaaaac catttttgtt aaaaagggag tttacgaaga
    16741 gaaagtattt atcggtaatc gctggcagga ctcaagaaaa gtaatcagct tgattggcga
    16801 aaatgttgac agcgtggtga ttgtgtggga agattaccac ggtaaaacca tcacctaccc
    16861 gggtagagag ggtacgattg tagccgacgg catgacggct gccaccttta cggtgaccgc
    16921 ccccgatttc tacatggaaa acattaccgt caaaaatccc agcaaggcag cacaggccga
    16981 agccatttat caaagcggag acaggcaggt attgaagaat tgtaagattc tcggtaatca
    17041 ggatacacac cgcaccaaaa aaggaaggcg gtatttttac tatcaatgta ccattgaggg
    17101 aggagtcgat tttatttacg ccggcggcac ctgttatttt tatcaatgcc agatcgtgag
    17161 caaccggaat ggttacatta cggcacccga agatattgta tatactgcaa gactgagcaa
    17221 cggtaaaatc ctgtactacg gatttatatt taacgactgc gacctcttgg ccaaagacgg
    17281 agcaaacgac gtttatctgg gacgtccctg gtcttcggaa tcgggcagta tttttatgaa
    17341 ttgccgcctg ggcagccata tcaatcgggc cggctggaat gcctggggcg ggaacgaaca
    17401 aaattgctcg tttgccgaat acaaaagcct caatgccgac ggatcggccc ttgccgacgt
    17461 atcaggaaga gtgtcgtgga gtatgcagct gagcgacaaa gaccgctata atttactggg
    17521 aatgaacacc atctaccggg ctgtttcatc gagcgctttt gatccggtct cgcaattcct
    17581 ccctttctcc ggccccaaat ctttgacggt cagtaacaga aaactaagtt ggacggccgt
    17641 cgaaaatgcg gtgggttatg tggtctacgc caacgatgcc gccatagact ttgccgaaac
    17701 aaacagttat tacgatctaa ttgaccgggg aacggtcaat tacaaagtga aagccatcgc
    17761 cggaaacggc cggctgagta ctttcaacgg actggccggc ggagtgacgg caaatgaact
    17821 ggattcgctg ctgaatccca aacactgggt aaatatatcc acctcggtag ctcccgattc
    17881 tgcaggagtg attctgcagg agccatctgc aaaacagcat cttgcgggca cacagataac
    17941 acttagcgct caacgcagat acggctatac atttataaag tggatcaacg caaacaacga
    18001 agttctttcg gaagaagaaa gctttgtact tacgctggac aaagacgaac aagtgacggc
    18061 tgttttcgaa aagcttcctt tgtattccct gaattgctcg gtaattggcg gaccacagga
    18121 actgattcaa ctctgtggtt ccgatacggt aatcgacggc gtcacttatt atgaagccgg
    18181 cacagaagtc actttgagcg ttgtcgatca cccactcttc cgtctcgact cctgggaaga
    18241 tgcctccacc gactcggttc gcatcatcac catgaacgaa aataaaaacc tcacggcacg
    18301 ttttcgttta caccccttta ttgtagcctg gacgttcaga atgcccggaa attcctctta
    18361 tagctccgat tattgcagcg acgaaaacaa tataggagaa tttaacctcc gtgatcaaca
    18421 ggggaatacg gccgattggt tgaattattc ccttttggag ggtggttacc tgggccgttc
    18481 ggctgtgctg aacggaaacg ccttgaacga tcgctattat tacgaagcga gcttttcgac
    18541 caaaggtcac tcggatatcg tattacgatc gtccatggcc gtagattcca acggctactc
    18601 tgtccaaaaa gcagaatatt ccacagacaa cggagcaagc ttcgaaacgc tgggagttat
    18661 cgatattgat tcaacaaaaa gctggaaaaa cagcgtcctg cctttacccg aaaatgcctg
    18721 ggatcaagat ttggtgcata tccgctggat acccgattat acctccgaaa tacatggaga
    18781 cccccaggga acggacggca cagccataac ggatatttac gtcttgctgc aagagaaaga
    18841 tgccctgccc aaggtaagcg gaagaattcc cctaaaggtc tggactgacg gcaacaaact
    18901 ggtttatttg caaagcgggc agcccgttcg tgtcgtcaat gtgtttgaca tgaccggtcg
    18961 ctgtgttgtc cggctttccg gcgaggaaat ccggtcggta aatttatccg ctctctccag
    19021 aggcatgtat gtattggcag cttacaacga aatcaacgag atggctgttg ccaagatcat
    19081 tttgaggtaa ttttcaacac tatacgctaa cattaagccg gacttatctt gtcagggtaa
    19141 ttttcaagtc acttataatg agatcttttt tatttaccat agtcgtttct tttatcctat
    19201 ccttgcccgt ttgctctcaa ccattggcat ttccgggtgc ggaaggatat ggtgctcaga
    19261 ccctgggcgg acgcggaggc gcggttcttt ttgtaagcaa tctgaacgac agcggccccg
    19321 gcagcctccg tcaggctgtt gaacaggaag ggccgcgtac ggttattttc aatgtttcgg
    19381 gcactatcga acttgaaagc gatctgacta tcggcaatcc ttacctgact attgccggac
    19441 aaacagctcc cggcgacggc atcacactga aaaattataa aatcaagctg gctgccgatc
    19501 acgtaattct tcgctatttg cgagtgaggc gcggcaacct gtccgaaaaa caagacgacg
    19561 ccatcaccat tcagaacgcc gaaaacgtta tagttgacca ttgttcgttt agctggggca
    19621 ccgacgaagt ggtcaacaca tggcatggtt ccaaaaactt aagccttcag tggtgcatca
    19681 tagccgaagg tctgcaccac aaagaacacg gatttgcagc aacactgggt ggcataaata
    19741 cttcctatca ccataatctt atcgccaatt gcccgggacg gaatcccagc gtagggggta
    19801 accacaacta tcaaacccat cgtatggatt tccgtaacaa cgttatcttc aatttcggcc
    19861 accgaacctt cgacggcaaa ccaagcagca tcaacatcgt caacaattac ttcaaacccg
    19921 gacccaacag cagcatcgat gtgttcgccc ggatagacga agccggtgtt tacaaagaaa
    19981 taccggaaag ttcctggtat atcgaaggta atgtatggga gggcaacgaa agcatttcac
    20041 gcgacaacag ccttggagtg aaaggcgcta ctcaatggat ggtagaccaa ccaaatgaat
    20101 tctcggctat aactacggaa caggccgaag cagcttattt atctgtttta gcgggagccg
    20161 gagcctgttt tcccaaaaga gatggcgtcg atttgagaat tctcgaggaa gcccgcaccg
    20221 gcaacaccac ctataacaag ggtgtggtga tgtccacttc ggaagtgggc gggtggcctg
    20281 tgttgaatag cctccccgcc cccgcagaca gcgatcgcga cggcatgccc gacgaatggg
    20341 aaaccaaaca cggacttgac ccctcaaacc ccgaagaccg gaacacaaca aacgcagaag
    20401 gctacaccat gctggagcaa tatttgaaca gtttggttca aaactgattt gagtttttcg
    20461 actcacttta tacctttatt acatttacag tttccaacaa aactgatata atccatttat
    20521 ttagacgatg aaaaagatca taatccttgt ttgtttatgt atggcttttt taagtgccaa
    20581 tgcgcaacag aaaaaaatct ggaacgaaag cccgaaagaa aaaaaagaac gtcttgcctg
    20641 gtggaccaac gaccgctttg gcttgtttat ccattggggg ccctactctc tggccgctcg
    20701 tcatgaatgg gtaaaaaaac gggaacaaat ggacgacaaa agctatcaaa aatacgtcga
    20761 ccatttcaat cccgatctgt accagccccg ggaatgggcc aaattggcca aaacggcagg
    20821 catgaaatac gcagtactca cctcgaaaca ccacgacggt tttgccatgt tcgacactaa
    20881 attcagcgat tataaaatca ccaacacact ttacggcaaa gatgctctaa aagaatgggt
    20941 tgaagccttc cgcgaagaag ggctgaaaat cgggttttat tattccctgc tcgattggca
    21001 tcatcccgag tacaccatag atgtcaaaca tcctcaaagc cccgaaaaca aggaagatta
    21061 cgaacgactc aaccgcggcc gcgacatgtc tgtttaccgc gagttcctca aaaaccaggt
    21121 cacggagttg ttgacaaatt acggtaaaat tgatctcttg tttctggatt tttctttccc
    21181 cgaagaagaa ttcggcaagg gatgtgagga ttggggctca gtcgaactca tgcaactggt
    21241 tcgcaaactt caaccctgga taatagtaaa cgaccgtatg gatttgagcg attattgggg
    21301 aggatgggac tttgccactc ccgagcaaca taaggtagat aaatggccta cctacaaggg
    21361 agaaaagatt ccctgggaaa cctgccaaac tttcagcgga tcctggggct actaccggga
    21421 tgaattaacc tggaaagaca acaaacaatt gctggtgctc ctgatagaat cggtaagcaa
    21481 aggaggcaat cttttgttga acgtagggcc taccggcagg ggcaccatcg actatcgggc
    21541 agaaaacgcc ctgcttaaaa tcggagactg gatgaaatac aacgcccgct ccatttacgg
    21601 ctgcacccag gctcccgatt cttttgagac tccggagaac accttattga cctacaatcc
    21661 cacgaccaac agactctacg ttcatttact cgattatccc ctgcgcaata ttaccctgaa
    21721 gggaatgaaa gggaaagtca actacctgca attcctgcac gatgcctccg aaattaaaat
    21781 atccacttca aaaggattca tagccatcga tcagggaagc acggaagaca acgacttgag
    21841 gttgagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggat tctaccttct
    21961 tgttgccatt cggccagtac tgagtccaga ctcctgtatt agtaagcaaa tcgcgttgcc
    22021 atttccattc aagcttcccc tctttatcgt aataaagctc ttcgcccgat ttgcggccgt
    22081 tttcatagac tgccacgtgc tgaatcgcac cgttttcgta ataatcggtc gtctcaccgt
    22141 gcaataaata ccggccattg gggcaaatgc gtgcactcca ggccgacttt aaccggccgt
    22201 ctttataagt ttcggtaaag ctctttactt cgccaccatc cgattccggg gtaatatcag
    22261 ccagatcgga ccatatccag gcttcgttca gttcgtagtg aatgggagga aagttggccg
    22321 tggtcagaat atgaataaca ccattaggtg cctgacgtgc ggttgaatag cccaacgatc
    22381 cgtgcggggg acgatggcgt tgcggcaatt gaaccggaag cggtttaata tgccaggttt
    22441 ctccattgtc tttggaaatg gcaactacgg cattgttccc gaattcccag ccggccggcg
    22501 gagcgatatt ttttttgtgc atgtaggagt ctgtcacata aaacaaacaa cccgaagcca
    22561 gacgtatcat agaaggacgc tgggcggtac ctaagggcgg gaagggtgaa gacgttcctt
    22621 cttcccagct tacgccccag tcgtgcgaaa tgttccgaac attccaaccc tcgatatcgg
    22681 cgttttttcc accctgtgca agcagagtgc ctttatcgtc gaggggtagt atggtcgaat
    22741 ggcggctgcc ggttcttcca cctttatctt tccagctttt tccctcatcg gcgctgtacc
    22801 acaaaaaact ttcactcccg tctccgtcgc aaaccatata tattccacct tggggatcac
    22861 ggaaagcatt tactaccggc tgagccgtat agtccgttgc tgccgtatca agctgaggaa
    22921 cagaaaaagt ccacgaggca ccgttgtccg tcgaaacagc tattctgaag gggacataat
    22981 cggagatgta tctgcctcct ccgaaaaacc aaattttgcc catgtcgttc catagcaaac
    23041 cggattggtc gttaccaaac tgtgtatcaa aaaagacttc gggcatatcc cagtcttccg
    23101 agccgtgacg aagcctggcc tgaacaaagg tggcagccgt gtcgctctcg ctcttcccgg
    23161 ccggcgaact gaaaaacaca gccagggcat cgccgttggg cataatctca aaccccggcg
    23221 aatggttgtg gtgatacact ccgtgttcta atcccgcaag tgtgcccacc tcgaaagggg
    23281 tgtatgcagg aggaatcgga acagcaaaac gcacggtaaa ataaggcagt tttgcatccg
    23341 gtccataagc cgcctgctcg ttggtttgtt ttacaacaga ctgattgaaa ggcactctgt
    23401 ctttttcggc aataacaaaa tgaatcaggt ttgccgaatc gcccaaagca atggtatttt
    23461 cgccggctct aaattgagag gcctttatat acagcgtgtc ttgccgtaaa tgcgcccact
    23521 gcttgctttt agtcggggaa catcctgttg tacagattat taagacggac agaattccca
    23581 tggattgaaa gagaagtttc ataggaaatg ttttttgttt ttaaccacac aaacgcccct
    23641 gtcacgcccg ttccttggca atctgctcca aggattttcc cgaagtatcc ggcataaaga
    23701 agaagccaac agagccgctg atcaaaagga agaccgacaa tagggctgcc accgggccga
    23761 taccggtttt ggccaaaatc ggcacaaaga agctccagac cccgagtatc gtccggctta
    23821 taccaaaggt tataccctgt gccgtacctc taagcatagt cgggaaaagt tcctggctga
    23881 acaccttgta gaaagcttcg ccggccagtg caccgccgga agaaaacatc agaatattgg
    23941 cgattataac cggaatggta aagggcaaaa agaggaacaa actataagct actacctgca
    24001 tcacggcccc aatgccccaa agcaattttc tggttttaaa attccggtcg gaataacgca
    24061 taaaaaccaa agaggttact attatggaaa taataaaacc tgcgctcgaa aggccaacgc
    24121 tgagagcctg cgaagaacct gcattaagtg tcttaataat gtaagggtta aaaatacctg
    24181 ccgtacctgc cgccaggttc cagaaaagat aaatagtggc cgtataagcc attcctctta
    24241 aattggagcc tttcaaaagt aagcgaagct ggctgccttt ggttttagca gctttagtcc
    24301 agcgggccga ttcagacagg ctgcgcctca aataccaggt aaccaaggcc accacaaata
    24361 aatgcaaaaa aacaatgcga atacccgtaa ctcccaggtc tgccaaaaac atagccaacc
    24421 agagcaccac cacagggccg aaagaccagg ccacctgtgt aaagccgagc agtttgccgc
    24481 gcgccttatc gggagccacc tcgcccacaa aggccagtga ggtgggtacg tcggcaccga
    24541 cggctactcc tactatgaag gtgccgacaa acaacatggt ggcattgaag cagaaagtaa
    24601 tcaataagac tccaaaagca taaaccaata agtctatttg ataaatgcgt ttacggcccc
    24661 atttgtcgcc cagccttccg ccaataatag agccgatggc acagcctatg gcgttgggtc
    24721 cgatggctgc cagcaaaccc acagcactgt ttgataaatt aaggtattct tgaaacaagg
    24781 ccagtcctgc ccccagtgcc acgattgaac cggcatccag gtaagaagcc attcccgcca
    24841 acgcagaggc tttccattgt tctttagtaa ttttttccgg atgggagtga ttctccttca
    24901 actcaacaag atccttgtta cttgctcctg acatactctt tttaattaaa attattggat
    24961 taattgttta ttattgcttt atgagctaca aattcccagc tgccggaacc cgcttctatg
    25021 acgaactgct tatcccccat ttttttaatg ccggataaat cttgtgcttt ctgggaataa
    25081 cttatttttt ccgcctccaa ataaatggta gctgtggtat tggacggtat tctgaactta
    25141 tatattatat catccccttt tatctcccaa gacgagagca aaagtccgta catagtctga
    25201 agacttgctt tggcataatt caatcgcctg gtgggacagg gcctgatgat actgtgtttg
    25261 taacccggtt tcgaagaatc gatgttcaga ccggccacag tgctgtaaag ccattggccc
    25321 accgcaccgt aagcgtagtg gttgaaagaa ttcattccaa cggtttgaaa agaaccgtcg
    25381 ggtttgatgc cgtcccagcg ttcccagatg gtggttgctc ccatggtaac gggataaagc
    25441 caggaaggat aacgtttatt gaacaataat tgataggcca ggtcgggata gccgttgtcg
    25501 ctcaacacac ggcaaagtaa gggagtacct aaaaaaccgg tggtcaggtg accaaaataa
    25561 cggacatcgt cggccagacg ttttgccatt gcttcacgct cctcttcggg tatcaggtca
    25621 aaacctaagg ccaatacata ggctgtttga gtccccgaaa ccaatcttcc gtttggagta
    25681 ataaactccc ggtgaaaagc ctcttttatc ttgggcagaa ggccggcata acgctcggaa
    25741 tcttcgttcc ggcccaggag cgaagctatc ttgctcatca gggaggtgga ataataaaaa
    25801 taagcggttg cgatcaggtc tttttcggtg tgggcgccgg cataaccgta atcgggagcg
    25861 ttgtaattat aggtgtaata ctcggcaaaa gacaaccagt ccccgaaatg gaaaccataa
    25921 tcaaaaatat agcgggaacc cgaatgcctg atcatatatt ccacccaggc tttcatcgat
    25981 tcgtattggt tctcgagtat ttgaacgtct ccgtaggatt ggtaaacagt ccatggaacg
    26041 attacggcag catctgccca gcccgtacct ccatatccgt cggaccagcc ggtgcctcct
    26101 ccgccttcga gcacgttggg aaccacccag ggcacgttac catcgtcttt ttggtcgagc
    26161 tgcaagtctt tcatccactt ggtaaagaaa gctcccgtgt tcatgttgaa acaggcagtg
    26221 ggagcgaatg cctgcgcatc gcccgtccag cccatacgct catcgcgctg ggggcaatcg
    26281 gtgggaacat ccagaaaatt gccccgcaag ccccattgaa tattttgttg taagcgattt
    26341 attaaaggat cggaacattc aaattcccca attttttcca tatcgctgtg taccacacgg
    26401 ccctctacat cgtcgaggct gatttcgccc ggatattcgc tgacacgcag gtatctaaaa
    26461 ccgtgaaaag taaaacgagg ctcgtattct tctatgcctt ctcctttaaa aatatagacg
    26521 tcttcggctt tggctactct aagattgtcg gtataaaagt tgccttcctg atccagcact
    26581 tcggcatggt gcaatacgat ggtctgtcca cgttctccct tcaggcgaaa gcgcacccag
    26641 cccaccaggt tctgtccgaa atcgaatacc aactctccgc ggggcgtcac aaatttcttg
    26701 acgggtttaa gggtttcggt aattcggacg ggctctcctt ccgttggaac caacacttcg
    26761 ttcgactgat tcagtatagt gactttttcc cacttggaat catcaaatcc cggctctgcc
    26821 caaccgggga tttcaagcct cgcatcgtaa aattcgccat gatatatctc cgatttgagg
    26881 aggggacctt taccgctttt ccaggacgga tcgctcaaaa tccattcttt ggagccgtct
    26941 tcgtaggtga tctccagttg agtaatcagg ccggccttat cgccgtaaag attacgttca
    27001 ccctgaaagc cgaaaaagcc gcgataccaa ccgtcaccaa cgatggctcc cagagcattc
    27061 cggccttgct tcaacaaggc agtaagatcg tacacctggt actgaatgcg tttactgtaa
    27121 gtggtccagc ccggattgaa ataatcttta cccagcttct ccccgtttag ataaagctcg
    27181 tataaaccaa gagccgaggc atacatcctg gccttggcca ctcgtttctt tacatcaaat
    27241 tctttcctga aaaagggaca aggattggac tgtgatgtaa cttcctccca gccgggggtg
    27301 atccaggcag ctttccattc gccggaagtc aataagccca tttcccagta agctgtctca
    27361 ccggaagtgg cctgattgcc ctgattatcc cataccgtaa cttgccagta aagcctctgc
    27421 cccgaaaccg attgcggtcc ctgatactcc acaaacaccg accggtcgga cgaaactaca
    27481 ccgctatccc acaccaattg attgcgtttt aatgcttcgg gagtggatgc caccttaatt
    27541 ctgtacgcgg tttgaagact gttttctttt tcagtatcca atctccagga tagcctggga
    27601 ttgaggtttg caatccccag gggattaatt ttgtattctg tccttaagtt tctgacattc
    27661 atcgttatgt ttaattaatg tttatcacgg gtagttgaaa aatcggttct catcgagacg
    27721 aaagcttgct ccgggactga aacaaagcct gaatgtctct ttcaatgggc tatcaaataa
    27781 aagaataggg cgcaatttag agaaaagttt taaaacatct ctgctaaaga ttgctaattg
    27841 tatttgtttt tgatttacaa ggttaattgg cgtatatatt aaatcgggga cacatcaaaa
    27901 ctttcttgca tacagggaat tgtttctatt aatcatcctt aaaaaattct atcctaccaa
    27961 aaagtcgcaa tcttttatca atttttacca atagaaaatt gccaaaaacc gctaatttta
    28021 cccaaatttg tactttactc ttgatttgtc aacacgttta actttttaat tctacctttt
    28081 atgaaaaaaa tcctgactcc tttattaacg accttattgt tgtttttggt ggcagtaccc
    28141 aaagcggatg ccatcatcac cgatcccaac gactacatta tttacgacag cggcattatg
    28201 gccgactcgg cgcattgctc cacctgggag ttccctccct ttatgacaat caccgacaat
    28261 gtaagcgtga aaaaagtaaa caagtggggg cgtcacaaca attctgttgt cgattacggt
    28321 tatcctccca aaacagacgt tgccacttta aagggcaaag ttgatctctg gggcatagtt
    28381 atcggccagg aaaagggcta cagcggtatc tcctacactg cctttttgga acaaccggaa
    28441 gaagaaagac ctctttttta tgtgaacaga tggaagggag gctggcttta tggacgtccc
    28501 accgactcta ttgttatccg tattcccact accagtgact caatctttat tcagggttgc
    28561 ggtacgaact ctttttatgc cattatggct tatgagcttc atcgcggaaa agattcagcc
    28621 tggtacatcg gcggtaactg ggggaatgtt tttaaccttg gattcaaacc caaacacgct
    28681 cccggcaccg actctacgga catcgttgtc ttaggcccgc acaaagactg gtatacgact
    28741 ggtgaagaat ggcaaggcaa cgccttccaa atgagattgg tcaatgctgc cgatcaaaac
    28801 gaagacggca cgatgaaaaa caaaaaatcg gagcgctggg gctttttcga aggcacctcc
    28861 atcaaccgga tcaaaatctt cggtcaaatc gaaaaaggat ccgttcctga atttgacggc
    28921 accattgccg gatgggactt cttatacagg cccaaagcga gcggaacagt cgacctgacc
    28981 tcttttgtta ccgacataga cggagaatgg gaagcagatt attattccct gtacgcctcc
    29041 gatctgggtg ttgttagggg aatgctcact gcttcttcac cttcggatgg catgcaattg
    29101 ggtaaagatt gcggtgtaaa agccgttatg atttctcctg ccggtaaaaa cgacacggtc
    29161 gatttcaccc tggccgaaga gcacgccaat tatttccaga ttgatgtttc tctgcaggat
    29221 ttccaggaaa tcaacctgaa ttttgacttc aacctacgcg atgctgccga cagttgtgtt
    29281 gtggcttatg ccgtagacgg tgcagataca tttaccgttg ccggaaaatt tgccgctccc
    29341 gaagattggg atggcttaac tcatgtatcg gtagatttgc ccgaactgac caatcaaagc
    29401 tatgtaacta tccgtattct gcaaaacaac ggagccctgg atgaaaacgg tcaattcata
    29461 ctggccaacc tctccataaa cggttttgat tacgtagaac acaaagaagg
//
LOCUS       DAOSTD010000016        28604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1815_length_28604_cov_14.135802, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000016 DAOSTD010000000
VERSION     DAOSTD010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 28604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28604
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1815_length_28604_cov_14.135802"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3425)
                     /locus_tag="PLI37_01935"
     CDS             complement(<1..3425)
                     /locus_tag="PLI37_01935"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOQ57811.1"
                     /translation="MKKKGFYWLFSLLAIVLLPLLLLNNNKFQQKLLQRYAQLLALEY
                     NLEPDFDSFHFQIDKNILIQFEGLRFADSLGQDFFRAERLDINARTLPLLLYKQLDIT
                     DIRLIGANIQVYRVYHDSPLNIAYLGRLFNFERQIFNSIRISSLLMKDCSFSYDVITA
                     PELSSDPLWADKEIFDVNHIAVKKLNTKLSLKIPRKAAPKLTMNSFSMEEAGGFVIND
                     LILKAIFDDNSLKMDNFSLVLPESSMGIDKLELAYDVDSGGHPKSFVLKDPVDVKLAL
                     NFSDITAFLPFDLQLDNSSADLAFTVVGGKDHLELNELYFQIDSVVYFNGNVNLNEIG
                     DEKQFKLLSTVDFLHLQPEALHLIQAVFSIQDSVIPPVVNNLGEMDFCGMVALEPENW
                     NITGEISADPGKLSILTQVEYSSSDAVYDFNLHLNSDSYALNKLFDSRITLGKTAFEI
                     DLNGKKAGIDPPIADLKAIFKTLQINNYTYRNIVLDAYYNGYGFYNANLNSGDKNCLL
                     DLAISLNDLGDEAAINCSLNAHRIDLQSLNWMPAAEEDKGSELSFNMQADLKGNTLQQ
                     LRGRLNIDSLRFENNGELFELDNFYALSGENGGERYVLLKSDLLEGWMYGYIDMTNLL
                     EDARNKALLQYFPTLFPDIKGDTRSMNNYDFRFKLDDTRSLSRALNLPLSLPSGMVFD
                     GYFRDESALFALNLNSEAIELDGWRIRSLKTTLDNPSDSLQWHFSGILEREDTSRINV
                     DFAARALNNELYWETHWHNDTGSVYKIYLSNILRFERENPKSPLSIFSALNPSEFVVR
                     DSLWKMESSDLQWDGKKLIVNNFLVHHNNQHIKLDGTLAAGNEEEFLVDLSEVNLDYI
                     FELLPRRREKPLLLGGSISGAAQIINLFEEPKLNASLSVENFSLNKAVLGDLNVNSSW
                     NEEISGIEMNGILLDGGLQVAEAKGAIFPAADSIYLWIDAEKARLSFIGSFIEDVIEN
                     LDGYGSGYVEIAGTFSNEKVGVTSDCYVEGGVIGVDLLHCNYYFNDSLHLTPQGIFFK
                     NINARDNEGNPAVINGTIRHNYFKDFALDLNIQTEKLKVFDIEPSYSEPFYGRVYATG
                     TAMLNGPLEDIQMDINMRTDERSSFAITLSEESEVSDYSFIEFVDRDKEAPKD"
     gene            3494..4516
                     /gene="tsaD"
                     /locus_tag="PLI37_01940"
     CDS             3494..4516
                     /gene="tsaD"
                     /locus_tag="PLI37_01940"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOQ57812.1"
                     /translation="MKSAYILGIESSCDDTSAAVLKDRSLLSNVIASQKIHKAYGGVV
                     PELASRAHEQNIVPVVDQALKQAGISKKDLSAIAFTLGPGLMGSLLVGTSFAKGLALA
                     LNLPLIQVNHLQGHVLAHFIQVEGKEKPDPNFPFICLLVSGGNSQLVLVHSVREMEII
                     GQTIDDAAGEAFDKCGKMLGLDYPAGPQIDRLATSGNPAAFRFSKPQIPGLDYSFSGL
                     KTSFLYRLRDEMAVNKTFIRDHRADLCASLQATIVDILMDKLIKASKDLNIKEIALSG
                     GVSANSGLRRAVEEIALLKGWTIHLPPLSFTTDNAAMIAVGGYYKFLDRDFCGMDAAP
                     YSRVGI"
     gene            4527..5795
                     /locus_tag="PLI37_01945"
     CDS             4527..5795
                     /locus_tag="PLI37_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOQ57813.1"
                     /translation="MDSSITPSYKNVEIITIGDEIINGQIVDSNSAWIASFLNRSGFE
                     VDKITSVPDDAQSIFRAVDSALGSSPIVLLTGGLGPTKDDITKHCLCSYFKTKLVFNQ
                     AVYDNMTGLFNKRGLRRINDFNRSQAEVPEACTVIVNRKGTAPVTWFEREGKILVSMP
                     GVPLEMMWCMEKEIMPRLLNKVQSHPILQRYIWVKGYPEAVLAEFISDWEDALPPELK
                     LAYLPQRSVMLLRLTAKNNDETTLIGLLETQLAGLRSRLGSAIFAEGVKTLEEALGQV
                     LIKKGLKLALAESCTGGAVGARLTRAPGSSAYFLGGIISYANELKQQWLGVPETLLQT
                     KGAVSKEVAEAMAAGVRIKLKSDIGIALSGIAGPTGGSAEKPVGTVFIALADNDRCLS
                     RKFEFQSDRLGNIEQSVHQALILLLDYLYL"
     gene            5956..6216
                     /gene="rpmB"
                     /locus_tag="PLI37_01950"
     CDS             5956..6216
                     /gene="rpmB"
                     /locus_tag="PLI37_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOQ57814.1"
                     /translation="MSKVCQITGKRAMIGNNVSHSKRRTKRKFNVNLFEKKFYWVEQD
                     CWIALTISAAGLRIINRKGLNQAIKKAGEDGFLRLEEVKILN"
     gene            6228..6416
                     /gene="rpmG"
                     /locus_tag="PLI37_01955"
     CDS             6228..6416
                     /gene="rpmG"
                     /locus_tag="PLI37_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOQ57815.1"
                     /translation="MSKKAKGNRVQVILECTEHKESGMPGTSRYITTKNRKNTTARME
                     LKKYNPILKKVTVHKEIK"
     gene            6425..6577
                     /locus_tag="PLI37_01960"
     CDS             6425..6577
                     /locus_tag="PLI37_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOQ57816.1"
                     /translation="MAKKTVATLHDVGSRNMVKVIKMVKTPETGAYSFQEEMVPVEKV
                     NEYVKK"
     gene            6657..7616
                     /gene="ftsY"
                     /locus_tag="PLI37_01965"
     CDS             6657..7616
                     /gene="ftsY"
                     /locus_tag="PLI37_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOQ57817.1"
                     /translation="MGIFSFFSKDKKEKLDQGLSKTKESFFTKVNRAIAGKSKVDDQV
                     LDELEEILIGSDVGVDTTLRIIERIVKRIERDKYLNTDELYSILREETVELLIENDQE
                     ASRDFIIPEDTKPYVIMVVGVNGVGKTTTIGKLAYQYTQRGKKVFIGAADTFRAAAIE
                     QLCVWGERAGANVVKQAMGSDPASVVFDTLNSALSNQADVVLIDTAGRLHNKINLMNE
                     LTKIKKVMAKLIPEAPHEILLVLDASTGQNAFEQAKQFSLATEVNALAITKLDGTAKG
                     GVVLGISDQFRIPVKYIGLGEGIDHLQIFDRKEFVNALFGSQF"
     gene            7617..8924
                     /gene="rimO"
                     /locus_tag="PLI37_01970"
     CDS             7617..8924
                     /gene="rimO"
                     /locus_tag="PLI37_01970"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOQ57818.1"
                     /translation="MNKKTPKVASVISLGCSKNLVDSEQLLYQLQRAGYRIKHNSDDA
                     EGGVVIINTCGFIQDAKEESIEIILQYAEARKRGNISRLFVMGCLSERYMEELKVEIP
                     EVDAYFGKFNWQNILSELGEVYHEANKNSRILTTPSHYAYIKISEGCNRDCSFCAIPL
                     ITGRHRSREIEDIAQEISDLTRNGVKEFQLIAQDLSYYGKDIYKDYQLAKLIETIADI
                     PGVEWLRLHYAYPQHFPYDILPIIRERDNVCKYLDIALQHCSDGVLQKMRRRIDYSST
                     LKLIERLRSEVPGIHLRTTVMVGHPGETEEDFQALKQFVQEQKFERMGAFAYSDEEGT
                     YAHRHYTDDVPQEIKQARLDEIMNIQQEIAASINREKIGQVYKVIIDREEPDFFIART
                     QFDSPEVDPEVFIEKTQALRIGEFYRAEIYSAGIYDLYGNIRS"
     gene            8940..10235
                     /locus_tag="PLI37_01975"
     CDS             8940..10235
                     /locus_tag="PLI37_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57819.1"
                     /translation="MKRFLVLIYGILLSLPFTAQNTTQGVTQNSSKSEIPTKTWDLYG
                     FIRSDFFVQNRNTRAVVLDLFPLYPMPKYPNGVGDDLNNSPSAGLSSITTRIGFKFNS
                     AGIFGAQKYTGIIETDFGGSSDYVLVRIRKAYSQLDWEKSRLILGQTWHPLFIEELMP
                     NVVSINTGAPFQALNRSPQIRYNYRGQDFNLTAAAIYQVMYTSSGPKGKSNQYQKDAV
                     IPNLFFGAEYNTGKLITGIGGDFKAIRPEKTWIDNSNIERINTRLLNTYSGIAYLKFA
                     HNRWSIISKTLLGQNLSDHSIIGGYIIDNEHNYLSLNTLASLAQVQYGQIHKLGLFGG
                     YSAILGSTASTNENIYNFYGFGAELIPSSNQYQYIRDMHRVMFTYTYNPKNWRMGVEL
                     EYNGVNWGSLNKNPSNQFRISADDYTAAYRMHAVIMYLF"
     assembly_gap    10237..10336
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10337..10815)
                     /locus_tag="PLI37_01980"
     CDS             complement(<10337..10815)
                     /locus_tag="PLI37_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57820.1"
                     /translation="MKKIALSLTAILLLCGCNTYAQVKNVRKAKARLNAETPNLVEAR
                     DAIEPALSDSITKTMADTWFTAGKIYYKLFDQEQKKEWSGAKADGELMAQSLAKCYDS
                     FVIADSLDQMPDKKGRVKPKFTKSITEMVQAMQNGFINSGSFYFKKEDYQSAIRMFE"
     gene            complement(10892..13423)
                     /gene="gyrA"
                     /locus_tag="PLI37_01985"
     CDS             complement(10892..13423)
                     /gene="gyrA"
                     /locus_tag="PLI37_01985"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOQ57821.1"
                     /translation="MIDEERVIRVNIEEEMKSSYIDYSMSVIVSRALPDVRDGMKPVH
                     RRVLFGMNELGNNSDKPYKKSARIVGDVLGKYHPHGDSSVYLTMVRLAQDWSMRYPLV
                     DGQGNFGSVDGDSPAAMRYTECRLRKIAEEMLIDIDKDTVDFQQNFDDTLKEPTVLPT
                     RIPNLLINGASGIAVGMATNMPPHNLSEIVDGTIAYIDNNDISIDELMKYVKAPDFPT
                     GGFIYGYSGVKEAFETGRGRVVIRAKADIESGPQHDKIIITEIPYLVNKADLIKSIAD
                     LTIDKRIEGISNVNDESDRSGMRIVVDVKRDANSNVVLNKLFKMTALQSSFSVNNIAL
                     VYGRPRLLNLKDLIRYFVDHRHDVVNRRTQYELNEAEKRAHILQGLIIACDNIDRVIA
                     LIRASDSPDDARAALMEAFDLSEVQARAIVEMRLRQLTGLEVDKLHKEFDELTQKIAW
                     YKEVLANVSLRMNIIKEELLEVKNKYGDPRRSEIIYASEDFNPEDFYSDDQMIITISH
                     LGYIKRTPLADFRSQNRGGVGSRGSETRDEDFVEYIYPATMHNTMMFFTQKGKCYWLK
                     VYEIPEGSKNSKGRAIQNLLNIDAEDKVNAFIRVTSLDDKEFNDTHYLIFATKQGIIK
                     KTPLEAYSRPRNNGVNAITIREDDQVIQVRLTDGNSEIILANRNGRAIRFHESNVRPM
                     GRTATGVRGMRIDEDGQDEVIGMIIVSEDESIMVVSEQGYGKRSEVDDYRITNRGGKG
                     VKTLNITEKTGKLVAIKSVNDENDLMIINKSGIAIRLTLADVRIMGRATQGVRLINLE
                     RHSDEIASVCKVTSEKEEELEIEDQDNSDEEEFSTNQNEQTADEN"
     gene            13665..16178
                     /locus_tag="PLI37_01990"
     CDS             13665..16178
                     /locus_tag="PLI37_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOQ57822.1"
                     /translation="MNTNKFSKDVLEILGFSNEEARRLGNAVIDPEHLLLGILREKNS
                     KALICLNKLNVDVAFVKQSIEKQIKQEKMIQNPVDLPLSASTERVLKMGLLEAHGMKS
                     EIMDTEHILLAILKEKNNLAANVLNNEGVQWETLRSLFYEKKYPTSGPEFTDEEDDDT
                     EEYGTGGKPDIFQKTQDRPGTGRDTPALDNFGNDLTKAALEGRLDPIVGREKETLRLA
                     QILSRRKKNNPVLIGEPGVGKSAIVEGLALRIVQRKVSRILFDKRIVSLDLASMVAGT
                     KYRGQFEERMKAVLNELQKNPDIILFIDEIHTLVGAGNASGSMDAANMLKPALSRGEI
                     QCIGATTLDEYRKSIEKDGALERRFQKIIVDATTLEETYLILKNIKDRYEEHHNVTYS
                     DEALRACVQLTNRYITDRNFPDKAIDAMDEAGSRVHISNVQVPESIELLEKEIDESNR
                     LKVKAVNEQNFELAADYRDKSKEQLKQLAILKADWESKLTENRQPVDEEKIAEVVAMM
                     SGVPLQRIAQEESKKLLRMKDELKSRIIGQDAAVEAVVKAIQRNRVGLKDPNKPIGTF
                     LFVGPTGVGKTHLAKLLAEAMFDTSDTLIRIDMSEFMEKFTVSRLVGAPPGYVGYEEG
                     GQLTEKVRRRPYSVLLLDEIEKAHPDVFNLLLQVMDEGRLTDSLGRRVDFKNTILIMT
                     SNVGTRQLKEFGKGVGFGYPDSIKQNDYSKSLIHKAITKTFAPEFLNRIDDTIYFEQL
                     SKDSILKIIDLELKAFYQRVESLGYTLDITPEAKNFIAEQGYDVQFGARPLKRAIQKY
                     LEDELAEVILSEKFKTNKHILVELDSAESKIILSNELEK"
     gene            16310..18163
                     /gene="htpG"
                     /locus_tag="PLI37_01995"
     CDS             16310..18163
                     /gene="htpG"
                     /locus_tag="PLI37_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOQ57823.1"
                     /translation="MQKGRIGVTTDNIFPVIKKFLYSDQEIFLREVISNAVDATQKLK
                     TLASVGEFKEELGDLSIRVSLNSKNKTLTVSDRGIGMTAEEIDKYINQIAFSGAEEFL
                     EKYKTDANAIIGHFGLGFYSTFMVSSRVEIVTKSWKEGSKAVRWSCDGSPEYELEEVE
                     KDDRGTDIILYIDNKDYLKEEKINELLNKYCKFLPIPIIFGKKKEWKDGKYQETDQDN
                     QINETEPTWTKKPGKLKEEDYLKFYKDLYPFSEDPLFHIHLNVDYPFHLTGILYFPKI
                     KNSIEIQRNKIQLYCNQVYVTDSVEGIVPNFLTLLHGVIDSPDIPLNVSRSYLQHDDN
                     VKKISNHITKKVGDRLKKIFKDDRKQFEEKWDDLKIFIQYGILTEEKFWDYAKEFSLM
                     QNIDGKYFTYEEYQTLIKEEQTDKDGQLIYLYASDKESQYAYIETAGNAGYDVLLMNG
                     ELDVPLMQHLEQKFEKSRFVRVDSDVIENLIQKKDREQLNLSEEEKESLKKLFSEQLP
                     AMDKVRFELQLDALGENSLPIVLTQNEFMRRMQDMSGLQPGLSFYADMPVSYSMVLNT
                     EHKLIKELIGKELDEKNKNTVNQLIDLALLSNGMLKGESLIKFIKRSCELI"
     gene            18219..20522
                     /locus_tag="PLI37_02000"
     CDS             18219..20522
                     /locus_tag="PLI37_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOQ57824.1"
                     /translation="MSFKRSILIAFCLSLVFYAQAQRVGLVLSGGGAKGLAHVGLIQA
                     LEENQIPIDYVTGTSIGAIVGSLYAMGLSPDEMMALFLSEDFTYWSTGTIQTKDIDYF
                     RKGDLSPEFFSTYLSLSDSLLSNPRSLVPGSLINASQLNYAFVQVYSQYTALCGGDFD
                     RLFVPFRCVATDVHSKQAVIFKNGRLEDAVRASMSFPLVFNAVSVDSMLLLDGGIYDN
                     YPVKLMQKEFNPDFVIGSNVSIVNSKPSEDDVVSLIEHIIIQPTHFEASPDEFIEFKF
                     DLKDVYLMDFHKALETYQKGYDLGISMIDSLKGIISRRQDPDSLAQKRQAFKANLPEL
                     IFDELELTGLNHQQEEFVRRMIGYDKDRRFSLKEFRKAYYAMIADTRISQIIPKVWYN
                     PSTGFFKLKLDIKTEKNLVFALGGNISSSTSNLLYARIGYQRFGKVATYYSADTYIGS
                     IHNALRLNTRWDVAGYIPSYLQASLSVSSFKFYEGEKLFYEELSPAFIQEREILFKLR
                     YGIPAFSQAKFEIGYSVGRISDWYMQSYLSNITRANFDRSYYDLSNFSLRLEKNNLNY
                     RQYPTGGSHYYLLCQYLLGEENYSYPDSVGNRFKESKELGYFQASAAYEGYLNFNKSI
                     TLGCILNAVYNNKRSLDNYTASIIQAPAFTPTPYTLSTFNEAFRSNKYAAIGIMPIWT
                     IRPFLHLRSEFYTFMPLDAIKRGPNQETVLSRSFSNTQFLGELSLVFHQPFVSMSLFT
                     NYLSYPKNNWNVGINIGFLIFNKRLVE"
     gene            complement(20617..20693)
                     /locus_tag="PLI37_02005"
     tRNA            complement(20617..20693)
                     /locus_tag="PLI37_02005"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20657..20659),aa:Arg,seq:acg)
     gene            complement(20794..22305)
                     /gene="dnaB"
                     /locus_tag="PLI37_02010"
     CDS             complement(20794..22305)
                     /gene="dnaB"
                     /locus_tag="PLI37_02010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOQ57825.1"
                     /translation="MDKSTTTTKRKKTSVNIADIGRLQPQALELEKAVLGALMLEKDA
                     FYEVSEILKDECFYEPRHQDIFKAIQQLAVKQKPVDILTVQEQLKQEGVLEEVGGAYY
                     LAELSSKVVSTAHLEYHARIVAQKHLARALISYTSEIQGDAYDEAMDVDELMQKAESK
                     LFELSQQNLKKDVIQINPVIDEALNILNLAAKREDGLSGIESGFHALDKITSGWQNSD
                     LIIIAARPAMGKTALALSMIRNMAVDNKQPVAIFSMEMSNVQLVNRLIVNVCEIEGEK
                     IKSGRLQDFEWYQLDQKIKDLYDAPIFIDDTPSLSVFEFATKARRLIREHQIKLIVID
                     YLQLMNASAKGLKYANRQEEVMTISRSLKALAKELNIPIIALSQVNRASANRAGIMGV
                     PVNEAKRPQLSDLRESGAIEQDADLVIFIHRPEYYRILEDENQNSLVGIAELIIAKHR
                     NGATGDIRLRFRSNFAKFSNLEDDAFFNGEDQPILSSRVNYQAGEDFDFDTDS"
     gene            22454..23272
                     /gene="ispE"
                     /locus_tag="PLI37_02015"
     CDS             22454..23272
                     /gene="ispE"
                     /locus_tag="PLI37_02015"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOQ57826.1"
                     /translation="MICFPNAKINLGLHVLEKRSDNYHNIETLFYPIGLKDILECVPV
                     DKTEHPLIQLSGIPLEGNPGENLVIKAYKLLQKDYPLKAVQIYLHKSIPSGAGLGGGS
                     SDAAFMLKLLNDIQHLGLSTSQLEHYAAQLGADCAFFIHNRPLLAFEKGDRFQVVDFS
                     LKGLFLVLLIPKNIRISTREAYAGIKPVKPGCKLTDCISKPPEYWKDCLVNDFEKTVF
                     VLNPELLHIKQALYEAGALYASMSGSGSSIFGLFRAECDLQDVFPDCYYWSEKL"
     gene            23361..23585
                     /locus_tag="PLI37_02020"
     CDS             23361..23585
                     /locus_tag="PLI37_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOQ57827.1"
                     /translation="MAKIKGAVLVDINRCKGCGLCVDSCPSQVLALAREVNEKGYNYA
                     YMAQPDTCIGCANCAYICPDGCIKVYKVKL"
     gene            23585..24661
                     /locus_tag="PLI37_02025"
     CDS             23585..24661
                     /locus_tag="PLI37_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOQ57828.1"
                     /translation="MMKGIHLMKGNEAIAHAAIRCGCDGYFGYPITPQSEILETLMEE
                     KPWESTGMVCLQAESEVAAINMVFGGASCGKKVMTSSSSPGVSLKQEGVSYLCGADLP
                     CLIVNIMRGGPGLGTIQPSQSDYFQSTKGGGHGDYHLIVLAPNSVQEMADFVGLGFDL
                     SFKYRIPALILSDGVIGQMMEKLKLPAFKPRATEEEIARDCPWALSGRTGMRPQNIIS
                     SLELDPAIMEKRNLERHERYRKVEATEVRYDTFMCDDAEFLIVAFGCAARICQKSVEL
                     LREQGIKAGLLRPITLWPFPKKAIESYCRQVRSILTVELNSGQMVEDVRLAVNGKVPV
                     GHYGRMGGIVPTPEEIVEVLKNNS"
     gene            24835..25605
                     /locus_tag="PLI37_02030"
     CDS             24835..25605
                     /locus_tag="PLI37_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOQ57829.1"
                     /translation="MNRSNNNSIIAPENLVYEKSKLLKDNVMHYCPGCSHGTVHRMIA
                     EVIEEMGMEDKAMGVSPVGCAVFIYKYINIDFAEAAHGRAPALATAMKRLRPDKLVFT
                     YQGDGDLAAIGTAETIHACNRGENITIIFINNGIYGMTGGQMAPTTLEGMKTSTCPYG
                     RNVALNGYPLKITNLLAELEGTCYVTRQSVHTAAAVKKTKRAIRKAFENSMEGKGTSI
                     VEIVSTCNSGWKMSPVQANQWMVENMIPFYPLGDLKDK"
     gene            25609..26145
                     /locus_tag="PLI37_02035"
     CDS             25609..26145
                     /locus_tag="PLI37_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOQ57830.1"
                     /translation="MKEEIIISGFGGQGVLSMGKILAYSALLENKEVSWMPAYGPEQR
                     GGTANVTVIISDELISSPILNSFDTAIILNQQSMDKFEPKIKPGGVLLYDPHGITRAP
                     VRKDIRIHTVDATEIASDMDNSKAFNMIVLGAYLKVLPIVQLKSILEGLRKTLPARHH
                     KLIPMNEQALQAGMDRVK"
     gene            complement(26211..27083)
                     /locus_tag="PLI37_02040"
     CDS             complement(26211..27083)
                     /locus_tag="PLI37_02040"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOQ57831.1"
                     /translation="MKFRLLLIATLFSITLFAQEKTEEAKSPWSYKGITGLNFSQTSF
                     TNWSEGGENSVAGNVYLNASLNYVHNKVSWTNDLSTNFGMYYTTENNWRKNIDNINFA
                     SKYGRSISKALYYAALLDFKTQFANGYNYAVSTTDRVSGFMTPAYLNLSVGIDYKPNE
                     HFSAYYSPVTGKMTFVSDTTFSTNYGLQAGKKNRAELGSSFRASANYDIFKVVNLKSS
                     LDLFTAYNQSFGKIDVNWEVLLNLKVTELLTVTFQSTLRFDDDIKYVDANGVLGPARV
                     QFKEVLGVGLSYKF"
     regulatory      27194..27387
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            27595..28347
                     /locus_tag="PLI37_02045"
     CDS             27595..28347
                     /locus_tag="PLI37_02045"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOQ57832.1"
                     /translation="MNTKHLVRSFTLGLTLIGMLACEPVVSKHEEEICIDFEQPVIEN
                     FQNNQVYAAKGLVFNNKYTLSEYEWEGKTYTSETWYGFSLSAMCDTVTEGFGNQYSCS
                     AGKAAGGKQFGLFYYSAYDADSSCFVQFEEEKEYVIKGMQITNSTYAYLSMKNGDDFA
                     KKFEAGDYLRIIFKSYDAEGKYLAQKTFYLADFRENKKTLLNSWEFVDLRELGKLNKL
                     SIFFESSDTGMYGINTPTYVCVDNLTYIKVQE"
BASE COUNT         8409 a         6108 c         6062 g         7925 t
ORIGIN      
        1 ctgtcttttg gggcctcctt gtcgcggtcg acaaattcta tgaaactgta gtcgctgacc
       61 tccgattcct cagacagtgt aatggcaaaa ctactgcgtt cgtcggtgcg catattgatg
      121 tccatctgga tgtcttctaa agggccattc aacatggctg tcccggtggc atacacccgg
      181 ccgtagaatg gttccgaata ggaaggttcg atgtcaaaaa cttttagttt ctcggtttgt
      241 atattcagat ccagagcaaa gtctttaaaa tagttatgcc taatggtgcc gtttattaca
      301 gccggatttc cctcgttatc tctggcattg atgtttttga aaaagatacc ttgaggcgtc
      361 aggtgtaggg agtcgttaaa atagtagtta cagtggagca aatctacccc gattactccc
      421 ccctccacat aacagtcgct ggttacacca accttttcgt tactaaatgt tccggctatt
      481 tcgacgtatc cgctaccgta accatccaaa ttttcaataa catcttcaat aaagcttcct
      541 ataaaagaga gtctcgcttt ttctgcatct atccacaagt aaatagaatc ggcggcaggg
      601 aatatagcac cttttgcttc ggctacctgt aatcctccgt ctaacaaaat gccgttcatt
      661 tctatgccgg aaatctcttc attccagctt gaattaacgt ttaaatctcc cagaactgct
      721 ttgttgagag aaaaattttc aacactcaat gaagcattta atttaggttc ttcaaataaa
      781 ttgattatct gtgcagcgcc gctgatactt cctcctaaaa gcaggggttt ttctctccgg
      841 cgaggcaata attcaaaaat gtaatccaga ttgacctcac ttaaatcaac caaaaactct
      901 tcctcattgc ctgcagctaa agttccatcg agttttatgt gttggttgtt gtgatgtacc
      961 agaaaattat ttactatcaa tttttttcca tcccattgta aatctgatga ttccattttc
     1021 cataaggagt ctctcactac aaattccgaa ggatttaaag cggaaaatat gcttaaaggc
     1081 gatttaggat tctcccgttc aaatcgcaaa atattgctca aataaatttt atatactgat
     1141 ccggtatcgt tgtgccaatg ggtttcccaa tacaattcgt tattcagggc cctggctgca
     1201 aaatccacat ttatcctgct ggtatcctcg cgttcaagta ttcccgaaaa atgccattgc
     1261 agggaatcgg aaggattatc aagcgtagtt ttaagagaac ggatgcgcca tccgtccagt
     1321 tcaatagctt cagaattcag attgagtgca aataatgccg actcatctct gaaataacca
     1381 tcgaaaacca tgccggaggg cagactcaaa ggtaaattca atgctcgtga caagctcctg
     1441 gtatcgtcaa gcttaaaacg gaaatcatag ttgttcatgc tgcgcgtatc cccctttatg
     1501 tccggaaaaa gggtgggaaa gtactgcaac aatgccttgt ttcgagcatc ttccagcaag
     1561 tttgtcatat caatatatcc atacatccaa ccttcgagca gatcagattt cagtaataca
     1621 tagcgttctc ctccattttc accgctcaat gcgtaaaaat tatccagctc aaacaattca
     1681 ccattgtttt caaaccgcag agaatctata tttaaacgtc ctctcagttg ttgcaatgta
     1741 ttacctttta aatcggcttg catattaaaa gacagttctg aacctttatc ttcttctgcg
     1801 gccggcatcc aatttaagct ttgtaaatca atacgatggg cattcagact gcaattgatg
     1861 gcagcttcgt cacccaaatc attcagtgat atggctaaat ccaataaaca atttttatcc
     1921 ccggagttca gattagcatt gtaaaatccg taaccgttgt agtaagcatc caatacaata
     1981 ttacgataag tgtaattgtt tatttgcagg gttttgaata ttgcttttaa gtcggcaata
     2041 ggggggtcta ttccggcttt tttcccgttt aggtctattt cgaaagctgt ttttcctaag
     2101 gtaatgcgac tatcaaataa cttgttcaga gcgtaggaat cagaattcaa gtgcagattg
     2161 aaatcataaa cagcgtccga agaagaatat tccacctgag tcaggatcga caactttccg
     2221 ggatcggccg atatttcacc ggttatattc caattttcag gctccagagc aaccattccg
     2281 cagaaatcca tttcacccaa attgttaacc acagggggta tgacgctgtc ttgtatcgaa
     2341 aaaacagctt gtatcaggtg aagggcttcc ggttgcagat gtaaaaagtc cacagtggat
     2401 agcaacttga attgtttttc atctccgatt tcgttcaggt ttacgttgcc attgaaatac
     2461 accacactat ctatttgaaa atagagttcg ttcaactcca agtgatcttt tcctccaaca
     2521 acggtaaatg ctaagtcggc actactatta tctagttgta aatcgaaggg caaaaaagca
     2581 gttatatcag aaaaattcag agccaattta acgtctacag gatcttttaa cacaaacgat
     2641 ttgggatgcc ctccagaatc gacatcgtaa gcgagttcca gcttgtctat tcccatgctt
     2701 gattcaggca aaaccaggct gaagttgtcc attttgaggc tattgtcgtc aaatattgct
     2761 tttaatatca aatcgttgat tacaaatcct ccggcttctt ccatggaaaa agagttcatt
     2821 gtcagctttg gggcagcctt ccggggaatt tttagcgaga gttttgtatt cagtttttta
     2881 actgctatat ggttgacatc gaagatttct ttatctgccc ataatgggtc tgacgacagt
     2941 tctggtgcag ttataacatc gtagctgaag gaacagtctt tcattaaaag tgaggatatc
     3001 ctgatggaat tgaagatttg acgctcgaaa ttgaaaagcc gccccagata agcaatattc
     3061 agtgggctgt cgtggtatac tctgtaaact tgaatatttg caccaataag cctgatatct
     3121 gtaatatcca gctgcttata gagcaaaaga ggcagagtcc gtgcgttaat atccaagcgt
     3181 tcggcccgaa aaaaatcctg ccccaaacta tctgcaaatc taagcccctc aaactgaatc
     3241 aatatattct tatctatttg aaaatggaaa gagtcaaagt ctggctccag gttatattcc
     3301 agagccagca actgggcata acgttgtaac agcttttgtt gaaacttatt gttgttcagc
     3361 aaaagaagcg gcaaaaggac gattgccaac aacgagaata gccaataaaa acctttcttt
     3421 ttcaagcgat atattgtact tttgcactcc ctaataatct gagagctacg aaccagcaaa
     3481 aataagaaaa tctatgaaat ctgcatacat tctggggata gaatcttctt gtgacgatac
     3541 ttctgctgct gttttaaaag atcgtagcct tttgtccaac gtcattgcaa gtcaaaaaat
     3601 tcataaagcc tacggcggag tagtacccga actggcttcc cgcgcccacg aacaaaacat
     3661 tgtacccgta gtggaccagg ctctaaaaca agcaggaatt agtaaaaaag acctgagtgc
     3721 catagctttt accctgggcc ccggtttgat gggttcctta cttgttggca cctcctttgc
     3781 taaggggctg gcccttgcac ttaatttgcc gctgattcag gtgaaccatt tacaagggca
     3841 tgttttggcc cattttatcc aagtggaagg taaagaaaag cccgacccca actttccttt
     3901 tatctgcctg ctggtatcgg gcggtaattc tcaattggtg ctggtgcatt ctgtcagaga
     3961 gatggaaatt attggacaaa ccatagacga tgccgccgga gaagcctttg ataaatgcgg
     4021 taaaatgttg ggcctggatt atccggccgg acctcaaata gatcgactgg caacaagcgg
     4081 taatcctgct gcctttcgtt tcagcaaacc gcaaattccg ggtttggact acagcttcag
     4141 cggccttaaa acttcttttt tatatcgttt acgcgatgaa atggctgtaa acaaaacttt
     4201 tatccgggat caccgagccg atttatgtgc ctccttgcag gccactattg tggacatctt
     4261 gatggacaaa ctcatcaaag ctagtaagga tttaaatatc aaagaaatag ctttatccgg
     4321 aggcgtatcg gcaaattccg gcttgcgcag agcggtagaa gaaatagccc tattaaaagg
     4381 ctggaccata catcttccgc ctttgtcctt cactaccgac aatgccgcca tgattgccgt
     4441 aggaggctat tacaaattcc ttgatcggga tttttgcgga atggacgcag cgccctattc
     4501 cagagtcggc atttaaatag accaccatgg acagtagtat tactccgtcg tacaaaaacg
     4561 ttgaaatcat cacgataggt gacgaaatca tcaacgggca aatagtagac agcaattcag
     4621 cctggatagc ctcttttttg aatcgctcgg gctttgaagt ggataaaata acttcggttc
     4681 ccgacgatgc acagtctatt tttcgggctg tggattcagc cctgggcagt tctccgattg
     4741 ttttgctgac aggcggctta ggcccaacca aagacgatat taccaagcat tgtctatgtt
     4801 cctatttcaa gactaaactg gttttcaacc aggctgttta tgataacatg accgggctgt
     4861 tcaataagcg gggcctacgg agaataaacg atttcaatcg ttcacaggcc gaagtccccg
     4921 aagcatgtac agtcatcgtc aaccgcaagg gcacagcgcc ggtcacctgg tttgaacgag
     4981 aggggaaaat tctggtttcc atgccgggag ttcctctgga aatgatgtgg tgcatggaaa
     5041 aagagatcat gccgcgcctg ctaaacaagg tacaatcgca tcccatcctt caacgataca
     5101 tttgggttaa aggctacccg gaagctgttt tggccgaatt tatttccgat tgggaagacg
     5161 ctttgcctcc ggagcttaaa ctggcttatc ttcctcagcg ttcggtaatg ctgttgcgcc
     5221 ttaccgcaaa gaataatgac gaaacaacat tgatcggtct tttggaaacg cagcttgccg
     5281 gcttaagaag ccggctggga tctgccatat ttgccgaagg ggttaaaacc ctggaagaag
     5341 ccctgggaca agtattaata aaaaaaggac ttaaattggc tttggccgaa agctgtacgg
     5401 gtggagcggt cggagccagg cttaccaggg ctcccggcag ttcggcttat tttttaggcg
     5461 gcatcatctc atacgccaac gagcttaaac aacaatggct tggggtgccg gaaaccctgt
     5521 tgcagaccaa aggagcggtg agtaaggagg tggctgaagc tatggccgca ggagtcagaa
     5581 ttaaattgaa aagcgatatc ggcattgcat tgagcggaat agccggcccc acaggaggca
     5641 gtgctgaaaa gccggtcggc accgttttta ttgccttggc cgacaatgac cggtgtttat
     5701 ccagaaaatt tgaatttcaa tcggataggc taggaaacat tgaacagtcg gtgcatcagg
     5761 ctttgatttt gttattggat tatttgtact tgtaaatctg tctcttaagc tcaaactgta
     5821 ttttttataa aatttattcc ctcatttttg gatatttgtc aaaaaaagat gatctttgca
     5881 cccgcaaaaa tctattcttg ttactcgcac acaagaggat gcaaaagcaa ttaaacacaa
     5941 aaacaacata cagcgatgtc aaaagtttgt caaattacag gaaaaagagc aatgattggc
     6001 aataacgtct ctcactctaa gagacgtaca aaacgcaaat tcaatgtcaa cttgttcgaa
     6061 aagaaattct attgggtaga acaagactgt tggattgcat tgaccatttc tgctgcagga
     6121 ttgcgcatca tcaaccggaa aggtttgaat caggctatca agaaggccgg ggaagatggt
     6181 ttcctgcgtt tggaagaagt aaaaattctt aattaggagg agtactcatg tcaaagaaag
     6241 caaaagggaa cagagttcag gttatcctgg agtgcacgga acataaagaa agcggtatgc
     6301 ccgggacttc ccgctatatc actactaaaa accgcaaaaa tacgactgcc agaatggaat
     6361 taaaaaaata caatcctatt ctcaagaagg tcactgtaca taaagaaatt aaataaatag
     6421 actcatggca aagaaaacag tagctactct gcacgatgtc ggtagccgta atatggtgaa
     6481 agttattaag atggtgaaaa cgcccgaaac aggagcctat tctttccagg aagaaatggt
     6541 acccgtagaa aaagtcaacg aatacgttaa aaaataaata gggctgttta tttctgacta
     6601 taaaggttgt tttttcacga aaacagcctt tttgtgcata tataacaagg gaatgtatgg
     6661 gtattttcag ttttttttct aaagataaaa aggaaaagct cgatcagggt ttatctaaaa
     6721 ccaaggaaag ctttttcacg aaagtgaatc gtgccatcgc cggtaaatca aaagtggacg
     6781 accaggtact ggatgaactg gaggaaattc tgattggatc cgatgtagga gtggatacta
     6841 ctctaaggat tatcgaacga atagtaaagc gcattgaaag agacaaatac ctgaataccg
     6901 atgaattata ctctattttg cgcgaagaga ccgttgaatt actcatagaa aacgatcagg
     6961 aagcttccag agattttatt atacccgaag acaccaaacc ttacgtcatt atggtggtag
     7021 gtgtcaacgg tgtaggaaaa accaccacca tcggcaaact ggcttatcaa tacacacagc
     7081 gaggcaaaaa agtgttcatt ggagcggccg acacattcag ggcggcagcc atcgagcaat
     7141 tatgtgtatg gggcgaaagg gcaggagcaa atgttgtaaa acaagcaatg ggctcggatc
     7201 cggcttcggt agtattcgat accctcaatt cggcgcttag caaccaggca gacgtggtac
     7261 ttatcgatac cgccggcagg ttacacaaca aaatcaacct gatgaatgaa ctgacgaaga
     7321 tcaaaaaagt aatggcaaag ctgattcctg aagctccaca tgaaattttg ctggttttgg
     7381 atgcatccac cggacaaaat gcctttgaac aggccaaaca attcagtttg gctaccgaag
     7441 tcaatgctct ggctattacc aaactcgacg gcacagccaa gggtggtgtg gtgctgggca
     7501 tctccgatca attcagaatt cctgttaaat acattggatt gggcgaaggc atcgatcacc
     7561 tccagatatt cgaccgcaaa gaatttgtaa acgctttgtt tggcagccag ttctaaatga
     7621 ataagaagac tcccaaagtg gccagtgtta tctcactggg ctgttccaaa aacctggtag
     7681 attcggaaca attgctatat cagcttcaaa gggccggtta tcgtatcaaa cacaattccg
     7741 acgatgccga agggggagtg gtcataatca atacctgtgg tttcattcag gatgccaaag
     7801 aagaatccat agaaatcatt ttgcaatatg cggaagcccg aaaacgcggg aatataagca
     7861 ggctttttgt aatgggctgc ttatcagagc gctacatgga ggaacttaaa gtagagatcc
     7921 cggaagtaga tgcctatttc ggtaagttca actggcaaaa catactgagt gaacttggag
     7981 aagtatacca tgaggcaaac aaaaatagcc gcatcctcac cacaccttct cattacgcct
     8041 atattaaaat ctcggaagga tgtaaccgcg actgttcttt ttgcgccata ccacttatta
     8101 ccggcaggca ccgctccaga gaaatcgaag atattgctca ggaaattagc gacttaacca
     8161 gaaatggagt gaaagaattt caactgattg cccaagatct atcctattac ggcaaggata
     8221 tctataaaga ctaccaatta gccaaattga tcgaaacaat tgccgatatt cccggagtag
     8281 aatggttgcg cctgcattat gcgtatccgc agcattttcc ttacgatatc ctgccgatta
     8341 ttcgcgaacg tgataatgtg tgtaaatacc tggatatagc tctacaacat tgcagcgatg
     8401 gagtgctgca aaaaatgcgc cgccgtattg attacagctc caccctgaaa cttattgaac
     8461 gccttcgcag tgaagtacct ggtatccacc tgagaaccac agtaatggtg ggacatccag
     8521 gagaaactga agaggatttt caagctctca agcagtttgt gcaagaacag aagtttgagc
     8581 gcatgggcgc ttttgcttat tccgacgaag agggcactta cgcccacagg cattataccg
     8641 acgacgttcc acaggaaatc aaacaagccc ggctggatga gatcatgaac atacagcagg
     8701 aaattgccgc ttccataaac agggaaaaaa tcggacaagt ttacaaagtc ataattgaca
     8761 gggaagaacc tgactttttt atagccagaa cccaattcga ttctcccgaa gtagatccgg
     8821 aagtatttat tgaaaaaaca caagcactta gaatcggtga attttaccgc gccgaaattt
     8881 atagtgcagg tatatacgat ttatacggta atattcgatc ttagtcaact cctttaaaca
     8941 tgaaaagatt tttggtattg atttatggta ttttactcag tttacctttc acggctcaaa
     9001 atacaaccca gggcgtaact caaaactctt caaaaagtga aattccaaca aaaacctggg
     9061 atttgtacgg ttttatacgc agcgattttt ttgtacaaaa ccgcaatacc cgtgccgttg
     9121 ttctcgattt atttccgctt tatcccatgc ctaaataccc aaatggagta ggggacgacc
     9181 tcaacaactc cccttcggca ggtttatcga gcataactac ccgtatagga ttcaaattca
     9241 attctgcagg tatatttggt gcccaaaaat atacaggcat catcgaaacc gactttggag
     9301 gcagctccga ctatgtatta gtgcgtatac gcaaggctta cagtcagttg gattgggaaa
     9361 aaagccgcct gatactgggt cagacctggc atccgctgtt tattgaagag ttaatgccca
     9421 acgtggtatc cataaatacc ggagcgcctt ttcaggcttt aaaccgcagc ccacaaatac
     9481 gctataatta tagaggtcag gattttaacc tgacagcagc agccatttat caggtaatgt
     9541 atacctcttc ggggcctaag ggaaaaagca atcagtatca aaaagatgcc gtcattccca
     9601 atttgttttt tggagccgaa tacaacaccg gcaagctgat tacgggtata ggaggagact
     9661 ttaaagccat acgtccggaa aaaacctgga ttgacaactc aaacattgaa cgcataaaca
     9721 ccagattatt aaacacatac tcgggtatag cctacctgaa atttgcccat aatagatggt
     9781 caatcataag caagaccctg cttggacaaa atttgagtga tcacagcata atcgggggat
     9841 acattattga taatgaacat aattacttgt ctctaaacac tttggccagc ttagcacaag
     9901 tgcaatacgg ccagatacat aaattgggcc ttttcggagg ttattcagcc attttgggca
     9961 gtacagcttc gacaaatgaa aacatttaca atttttacgg ttttggcgcg gaattaattc
    10021 cctccagcaa tcaataccag tacattcggg atatgcatcg tgtaatgttt acatatacct
    10081 acaatcccaa gaactggcgc atgggagttg aattggaata caacggagtc aactggggga
    10141 gcctgaataa aaaccccagc aatcaattta gaatttctgc cgatgattat acggccgctt
    10201 accgaatgca cgctgttata atgtacttgt tttaaannnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnntatt cgaacatcct gatggcactt tgataatcct cctttttaaa
    10381 ataaaaagag ccggaattaa taaaaccatt ttgcatggct tgtaccattt ccgtaatgga
    10441 tttggtaaac ttaggcttca ctctgccctt cttatcgggc atttgatcca aagaatcggc
    10501 tatcacaaag ctatcgtaac attttgccag tgactgtgcc attaattctc cgtcagcttt
    10561 ggcgccgctc cattctttct tttgttcctg gtcgaacagt ttgtaataga tcttacctgc
    10621 tgtaaaccag gtgtcagcca ttgttttggt gattgaatcg ctcaatgcag gctcaatggc
    10681 atctctggct tcgacaaggt taggtgtctc ggcattcagt ctggctttgg ccttgcgcac
    10741 gtttttaact tgtgcataag tattacatcc acacaataaa aggatggctg taagtgataa
    10801 agcaattttt ttcatacgat tcatttttta atattgttga tgttttagat acaaatatac
    10861 atctgaattt gattctgtca atagaaaaga attaattctc gtctgccgtc tgttcatttt
    10921 gatttgttga aaattcttct tcgtctgaat tatcctgatc ttctatttcc agctcttcct
    10981 ctttttccga agtaacctta caaactgaag cgatctcatc gctatgcctt tccaaattga
    11041 tcaggcgaac tccctgcgtg gctctaccca tgatgcggac atcggccaac gtaagtcgga
    11101 tagctatacc ggatttattt atgatcatca gatcgttttc gtcgttcacc gacttaatgg
    11161 ctaccaattt gccggtcttt tcagtgatat tcagggtttt aacacccttc cctcccctgt
    11221 tggtgatacg ataatcatcc acttcggaac gtttgccgta gccttgttcg gaaaccacca
    11281 taatgctttc gtcttccgaa acaataatca ttccaataac ttcgtcctgg ccatcttcat
    11341 cgatacgcat accgcgtacg cctgtagccg tgcggcccat gggtctgacg ttgctttcat
    11401 ggaaacgaat ggcgcgtccg tttctgtttg ccagaatgat ttcactgttt ccgtcggtca
    11461 gacgaacctg aatcacttga tcgtcttcgc gtatggttat tgcatttaca ccattgttcc
    11521 ggggtctgga ataggcttcc agcggagttt tcttgataat accctgcttg gtggcaaaga
    11581 tcagatagtg cgtatcgttg aattctttgt cgtcaaggct tgtcacacgg atgaaagcat
    11641 tcactttgtc ttcggcatca atgttaagca gattttgaat agcccggcct ttggaatttt
    11701 tggaaccctc gggaatctca tacactttaa gccagtaaca cttacctttt tgagtaaaaa
    11761 acatcatggt gttgtgcatg gtggccggat aaatgtattc tacgaagtct tcatcgcggg
    11821 tttcactgcc tcgggaacct accccgcccc ggttttgaga cctgaaatca gccaaaggcg
    11881 tccgttttat gtaacccaga tgtgaaatag tgataatcat ttgatcgtca gaatagaaat
    11941 cttcgggatt gaaatcttcc gaagcgtata tgatttctga acgcctggga tcgccgtatt
    12001 tattcttgac ttccagcaat tcttccttga tgatattcat tctcaaactc acattggcca
    12061 agacctcttt gtaccaggca attttctggg ttaattcatc aaattccttg tgcaatttat
    12121 caacttccaa tccggtaagc tgacgcagcc tcatttcaac tatagcccgt gcctgcactt
    12181 cgctcaaatc aaaagcctcc attaaggcag ctctggcatc gtccggagaa tcagatgctc
    12241 taataagggc gatgacgcgg tcaatattgt cgcaagctat aatcaagcct tgcaaaatat
    12301 gagcccgttt ttcagcttcg ttcaattcgt attgagttcg gcggtttacc acatcgtgcc
    12361 tatggtccac aaaataacga atcaggtcct taagattcaa caaacgcgga cgcccataca
    12421 ccaacgcaat gttattaaca ctgaacgaag actgcaaagc tgtcatctta aacagtttat
    12481 tgagtacaac attggaattg gcatcgcgtt ttacatctac gacaatgcgc ataccgctgc
    12541 gatcagactc gtcgttaaca ttcgaaatac cttcgattct tttatctatt gttaggtcgg
    12601 caatgctttt gatgaggtct gccttgttga ccagataggg aatttcagtt ataattattt
    12661 tatcgtgttg cggaccactc tcaatatcgg ccttggcgcg aatcacaaca cggcctcttc
    12721 ctgtctcaaa agcttccttt acgccagaat acccataaat aaagccaccg gtggggaaat
    12781 cgggagcctt gacatatttc atcaattcgt ctatagagat gtcgttgtta tcgatatagg
    12841 caatagttcc gtccacaatt tccgataaat tatgcggcgg catattggtg gccataccta
    12901 cggcaatacc tgaggctccg tttataagta ggttgggaat acgggtgggt aaaaccgtcg
    12961 gttccttaag tgtatcgtca aagttctgtt gaaaatcgac ggtatctttg tcgatgtcaa
    13021 tcaacatttc ttcggcaatt ttgcgcaaac gacattcggt gtaacgcatg gcagccggac
    13081 tgtcgccgtc aaccgaaccg aagttgcctt gtccgtctac caaaggataa cgcatagacc
    13141 agtcctgggc gagacgtacc atggttaaat aaacagaaga atctccgtgg ggatggtatt
    13201 taccaagcac atcacccaca attctggctg atttcttgta aggtttatct gaattattgc
    13261 ctaattcatt cataccaaac agcactctgc gatgtaccgg cttcataccg tcacgtacat
    13321 cgggcaaggc tcgggaaact atgaccgaca tagaataatc tatgtaagaa gatttcattt
    13381 cttcctcaat gttaaccctt ataactcttt cttcatcaat catttgataa aaatcttaac
    13441 agttaaaaca aaaacacgct aatgtagcac tttttaaaaa actgggcaaa aaaaaacagc
    13501 aataaattca aggaatcaag ccactttcaa gacaatctat actgacattt tgtcctttgg
    13561 caagtttttt gctcatagta aggaaaaact gatagaaaac aagtacattt gaccgtataa
    13621 atgtttatct taggttttac attttgaaca gaaagattat aacgatgaat acaaataaat
    13681 tttccaaaga tgtactcgag attttagggt tcagtaacga agaagcccgc aggttgggca
    13741 atgctgttat tgatcctgag catcttttgt tgggaatatt gagggaaaaa aacagcaaag
    13801 ccctgatttg cctcaacaaa ctcaatgtgg atgtggcttt tgtgaagcaa tccatcgaga
    13861 aacagatcaa gcaggaaaag atgattcaga atcccgttga tttacctctg agcgcatcta
    13921 ccgagcgagt tctcaaaatg ggcttgctcg aggcacacgg aatgaaaagt gaaataatgg
    13981 atacagaaca tattcttttg gccattctaa aagaaaaaaa caatttggca gccaatgtgc
    14041 tcaacaacga aggagtacaa tgggagacct tgcgcagcct gttttatgaa aaaaaatacc
    14101 ctacttcggg gcctgagttt accgacgaag aagacgatga caccgaagaa tacggtaccg
    14161 gcggcaaacc tgacatattt caaaaaaccc aggacagacc gggtaccggc agggacactc
    14221 ctgctctgga caattttggc aacgatctta caaaggctgc tttggaaggc aggctcgatc
    14281 ccattgtcgg gcgcgaaaaa gaaaccctga gactggccca gattctgagt agaagaaaga
    14341 aaaacaatcc ggtacttatc ggagagcccg gcgtagggaa atcggccatt gtagaaggtt
    14401 tagccctgcg catagtacaa cgcaaggtgt cgcgcatatt gtttgacaaa cggatcgtat
    14461 ctcttgatct ggcctccatg gtggccggta ccaaataccg gggacaattt gaggaaagga
    14521 tgaaagccgt tctgaacgaa ttgcaaaaaa atcccgatat cattttattt attgacgaaa
    14581 tccatacttt ggtcggcgcc ggcaatgcca gcggctccat ggatgcggcc aatatgctga
    14641 aaccggcctt atcgagaggt gaaatacagt gtataggagc cactacgctc gatgaataca
    14701 gaaaaagcat cgaaaaagac ggagccctgg aacgccgttt ccagaaaatc attgtcgatg
    14761 ccactactct cgaagaaacc tatttgattt taaagaatat caaagatcgc tacgaagagc
    14821 atcacaacgt cacctactct gacgaagccc ttagagcctg tgttcagctt accaaccgct
    14881 atattaccga ccgtaatttc cccgataagg ccattgacgc catggacgaa gccggttcca
    14941 gagttcatat ttccaatgta caggttccgg agagcataga gttgctcgaa aaggaaatcg
    15001 acgaaagcaa cagactcaaa gtgaaggccg tcaacgagca aaactttgaa ctggccgccg
    15061 attatcgcga taaaagtaag gaacagctca agcagttagc aatccttaaa gcagattggg
    15121 aatccaaact caccgaaaac cgtcagccgg tggacgaaga aaaaatagcc gaagtggtag
    15181 ccatgatgtc cggggttccc ctgcaacgta ttgctcagga agaaagcaaa aaattgctaa
    15241 gaatgaagga cgagcttaaa agtcgcatta ttggacagga cgctgctgta gaggctgttg
    15301 taaaagctat acagcgcaac cgggttggat tgaaagatcc caacaagccc attggaactt
    15361 ttctttttgt cggcccgacc ggtgtgggta aaacccactt ggccaaattg ctggccgaag
    15421 caatgttcga caccagcgac acgcttattc gcatagatat gagcgagttt atggagaaat
    15481 tcaccgtatc acggctggta ggagcccctc ccggatacgt aggctacgaa gaaggaggcc
    15541 aactcaccga aaaagtccgt cgcaggccct actccgtttt gttacttgac gaaatcgaaa
    15601 aagcccatcc ggacgtcttc aacctgttgc tgcaggtgat ggacgaaggc cggctgaccg
    15661 acagcctggg acgcagagtg gatttcaaaa acaccatttt aattatgact tccaacgtgg
    15721 gcactcgtca actcaaggaa ttcggtaaag gcgtaggctt tggctatccg gattccatca
    15781 aacagaatga ttattctaaa tctttgattc ataaagcaat aaccaaaaca tttgctcctg
    15841 aatttttgaa tcgtatcgac gatacgattt attttgagca attgtccaaa gattctatct
    15901 tgaaaattat tgatctggaa ttgaaagcct tctatcagcg ggtggaaagc ttaggttata
    15961 cactagacat tacacccgaa gccaaaaatt tcattgccga acaaggttat gatgtgcaat
    16021 ttggtgcccg ccctctgaaa agagccattc aaaaatacct cgaagacgaa ctggctgaag
    16081 tgattttgag tgagaaattc aaaacaaaca agcatatttt agtcgaattg gatagtgctg
    16141 aaagcaagat tattctctcg aatgagcttg aaaaataaaa atagctgcca aaatagcagt
    16201 caaaatcaaa aaatgtcatt ccctggtgtt tggcattttt tttgttccaa atgagataga
    16261 aaaacagctt tattcattca ttttatcgta tttttaaata tatcaaatta tgcaaaaagg
    16321 acgtattggg gttactacag acaatatctt ccccgtcata aaaaagtttt tatacagcga
    16381 tcaggaaatt tttttacgtg aggtcatttc aaatgccgtc gatgccactc agaaactcaa
    16441 aacactggct tcggtgggag aatttaaaga agaactcggg gatttatcca ttcgcgtaag
    16501 cttaaacagc aaaaacaaaa ccttgaccgt ttccgaccgt ggaattggta tgactgcgga
    16561 agaaatcgat aaatacatca accaaatagc cttttcagga gctgaagaat ttctcgaaaa
    16621 atataagacc gatgctaatg ccattattgg ccacttcggt ttaggttttt attctacttt
    16681 tatggtttcg agccgggttg aaattgtaac caagtcctgg aaagagggat caaaagctgt
    16741 gcgctggagt tgcgacggat cgcctgagta tgaactggaa gaagtcgaaa aagacgaccg
    16801 tggcaccgat attatcctgt atatcgataa taaagattac ctgaaagaag aaaaaataaa
    16861 cgaactgctc aataaatact gtaagttcct gcccataccc atcatctttg gcaaaaagaa
    16921 agaatggaaa gatggaaaat accaggaaac tgatcaggat aaccaaataa acgaaactga
    16981 accgacctgg accaagaaac ccggcaaact gaaagaagaa gattatttga aattttacaa
    17041 agacctctac cctttcagcg aagatccgct attccatatt cacctgaacg tggattatcc
    17101 ttttcatctg accggtatat tgtatttccc caaaattaaa aacagtatag aaattcaaag
    17161 gaacaaaatt cagttgtact gcaaccaggt ttatgtgacc gattccgtag aaggcattgt
    17221 gccaaacttc ctgactttac tgcacggagt tatcgattcg cccgatattc cattgaatgt
    17281 atcccgttct tacctccaac acgacgacaa cgtaaaaaag atatccaatc atatcaccaa
    17341 aaaagtaggc gatcgcctga aaaagatttt caaagatgat cgtaaacaat ttgaggaaaa
    17401 atgggacgac cttaagatat ttattcaata cggtattttg acggaagaaa aattctggga
    17461 ctatgccaag gaattcagtc tgatgcaaaa tattgacggc aaatatttta catacgaaga
    17521 ataccagacc ctgatcaaag aagaacaaac agacaaggac ggtcagctta tttatctgta
    17581 cgccagcgac aaagaaagtc aatacgccta tattgaaaca gccgggaatg ccggttacga
    17641 tgtcctattg atgaatgggg aattggacgt acctctgatg caacacctgg aacaaaaatt
    17701 cgaaaagtcg cgttttgttc gtgtagacag cgatgtcatc gaaaatttga tccaaaaaaa
    17761 ggacagagaa cagcttaatc tgagtgaaga ggaaaaagaa agtctgaaaa agttgtttag
    17821 cgaacaatta cctgcaatgg acaaagttcg tttcgaattg caattggacg ctcttggcga
    17881 aaacagtctg cctattgtgc tcacccagaa cgaatttatg cgtcgtatgc aagatatgtc
    17941 cggcctgcaa cccggtttga gtttttacgc agacatgcct gtgagttaca gcatggtttt
    18001 gaatacagag cataaattga tcaaggaact cattggaaaa gaactcgacg aaaagaataa
    18061 aaatactgta aatcaattga ttgacctcgc tctcctgagt aatggcatgc tcaagggcga
    18121 atcactgata aaatttatta aaaggagttg tgagctcatt taaccgggat aattatcgta
    18181 cttttgataa ctttgtgtta ccaaatctgc gataataaat gagctttaag cgcagcatac
    18241 ttattgcctt ttgtctgagc ctggtatttt atgcccaggc tcagcgcgtt ggtcttgtat
    18301 taagtggcgg cggagcaaaa ggcctggcac atgtggggct gatacaagcc ctggaagaga
    18361 accaaattcc catcgattat gtcacaggga cctcaattgg tgccattgtg ggcagcctgt
    18421 acgccatggg cttgtctcct gacgaaatga tggccctgtt tttgtcggaa gactttactt
    18481 actggtcaac cggaaccatt caaactaaag acatcgacta ttttagaaaa ggagatttaa
    18541 gcccggaatt cttttctacc tatctttctc ttagcgacag ccttttgtcc aatcccagat
    18601 ctttagtacc cggcagcctg attaatgcca gccaactcaa ctacgctttt gtacaggttt
    18661 atagtcagta tacagccttg tgtggaggtg atttcgatcg attgtttgtc ccttttcgtt
    18721 gcgttgcaac cgatgtacac agcaaacaag ccgtaatttt taaaaatggc agactggaag
    18781 atgccgtacg cgcttccatg agtttccctt tggttttcaa tgccgttagt gtggacagta
    18841 tgcttttgct cgacggggga atctacgata attatccggt gaagctcatg caaaaagaat
    18901 tcaaccccga ttttgtcata ggtagcaacg tttctatagt taacagcaag cccagcgaag
    18961 acgatgtggt ttcgctaatt gaacatatta taattcaacc cacacatttt gaagcttctc
    19021 ccgatgagtt tatagaattc aaatttgact tgaaggacgt gtacctgatg gattttcaca
    19081 aagccttgga aacctatcaa aaaggctacg atttagggat ttctatgata gactccctta
    19141 agggaataat ctccagaagg caagatccgg attcattggc tcaaaaaaga caggctttca
    19201 aagcaaacct tcctgagctt attttcgacg aactcgaact caccggtctc aaccatcaac
    19261 aggaagaatt tgtacgcaga atgatcggct acgacaaaga tcgcaggttc tcgctcaaag
    19321 agttccgcaa ggcctattat gccatgatcg ccgataccag aatatctcag atcattccca
    19381 aagtctggta caatccttcc accggatttt ttaaactgaa actggatata aaaaccgaaa
    19441 agaacctggt attcgctttg ggcggaaaca tctcttcttc gacctccaac cttttgtatg
    19501 cacgtatagg ttaccaacga tttggcaaag tggccaccta ttactcagcc gacacataca
    19561 taggcagtat ccacaacgct ttgagattga acacgcgatg ggatgtggca gggtatattc
    19621 ccagttattt gcaagccagt ctttcggtaa gcagcttcaa gttctacgaa ggtgaaaaat
    19681 tattttacga agaattaagc ccggctttta tacaggaaag agaaattctt ttcaaacttc
    19741 gttacggtat acctgcattt agtcaggcta aatttgaaat tggctattca gtgggaagaa
    19801 tcagcgattg gtatatgcaa tcgtacttaa gcaatatcac tcgagccaat tttgaccgca
    19861 gttactacga cttgtcgaat ttttcccttc gtttggaaaa aaacaattta aattacaggc
    19921 aatacccgac gggaggcagc cattactatc ttttgtgtca atatttgctg ggggaagaaa
    19981 actactctta tcccgactct gtggggaacc gcttcaagga aagcaaagaa ttgggttatt
    20041 tccaggcttc ggctgcctat gaaggctatt taaattttaa taaaagtatt actctgggct
    20101 gtatactaaa tgctgtttac aataacaagc gttctttgga taactataca gctagcatca
    20161 tacaggcacc ggcttttact cccacaccct acaccttgag tacttttaat gaagcctttc
    20221 gttctaacaa atacgctgcc ataggaatta tgcctatttg gaccatacgt ccttttttac
    20281 acctgagatc tgaattttac acctttatgc ctctagatgc tatcaaacgg ggacctaatc
    20341 aagaaacagt gctgagcaga tctttttcga atactcaatt ccttggtgag ttatctctgg
    20401 tgtttcacca gccttttgtg tccatgagtc tgtttaccaa ctacctgagc tatcccaaaa
    20461 ataattggaa cgttggcatc aacattggtt tcctaatctt caacaaacgt cttgtcgaat
    20521 gatggatttt taggattatt ggcaaagtga tgggggaaaa ttaaaaaagc tacctggctg
    20581 attttacgcc ttgtagcctt gttcttctcg ggtacgtggt gaccccgaca ggattcaaac
    20641 ctgtaacctt ctgatccgta gtcagatgct ctattcaatt aagctacggg gcctttttta
    20701 acgggcacaa aagtacattt aaatattgat tagcacaatt attattccct cttttttgat
    20761 ttctccttaa aaaactttgt ctggattgtg tgcttagctg tctgtgtcaa aatcaaaatc
    20821 ctctccggct tggtaattga ctcttgaact gaggatgggc tgatcctctc cgttgaaaaa
    20881 ggcatcgtct tcgaggttgc tgaatttggc aaaattgctt ctgaaccgta gtcgaatatc
    20941 tccggtagca ccgtttctgt gcttggcaat aatgagttcg gctatgccca ccaggctgtt
    21001 ttggttctcg tcttccaata tgcggtaata ttcgggccgg tgaataaaaa taaccaagtc
    21061 ggcgtcctgc tcaatggctc ccgattcacg caaatcagat aattgaggcc tttttgcttc
    21121 attgacggga actcccatga ttccggcacg gttggcagaa gcgcggttca cctgagaaag
    21181 cgcaatgata ggtatattca attctttagc taatgccttg agtgaacggg aaatagtcat
    21241 aacttcttcc tgtctgttgg catactttag ccccttagca ctggcattca ttaattgcag
    21301 gtagtctatc acgataagtt tgatctggtg ctcccttatc aaccgtctgg ccttggtagc
    21361 aaattcaaaa actgacagac tcggtgtatc atcaataaaa atgggagcat cgtagagatc
    21421 tttgattttt tggtccaatt gataccattc aaaatcttgt aaacgcccgc ttttgatttt
    21481 ttctccttca atttcgcata cattaacaat caggcggtta actaattgca cattggacat
    21541 ttccatagaa aagatagcca ccggctgttt attgtccaca gccatattcc tgatcatcga
    21601 caaggccaga gcggttttac ccatggcagg cctggcagct ataatgatca ggtccgaatt
    21661 ctgccagccg gaagtaatct tgtccaaggc atgaaaaccc gattcaatac cgctcagacc
    21721 gtcttctctt ttggcggcca ggttcaggat attcagggcc tcgtcgatga cgggattgat
    21781 ttgaataaca tcttttttaa gattttgctg ggagagctcg aacaatttgg actcggcctt
    21841 ttgcatcaac tcatccacat ccatggcttc gtcataggca tctccctgaa tctcggaagt
    21901 atagcttatc aaagccctgg ccaggtgctt ctgggcgaca atacgggcat gatattccaa
    21961 atgcgcggta gaaaccacct tggaacttaa ttcggccaga taataagccc ctcctacttc
    22021 ttccaaaacc ccttcctgtt ttagttgctc ctggacggta agaatgtcta ccggtttttg
    22081 cttgacggcc aattgttgta tcgccttaaa aatatcctga tgccggggtt cataaaaaca
    22141 ttcgtctttt aaaatttcgc tcacttcata aaaagcatct ttctcgagca taagagcccc
    22201 taataccgcc ttttcaagtt caagagcctg aggttgaagt cggccaatat cggctatgtt
    22261 gacggaagtt tttttgcgtt tggtagtagt ggtagattta tccattctgt tgaatttgat
    22321 gtcttcaaaa gtacagaaga aataactgat gcccaaatat acttatcaac attcttttaa
    22381 ccggaatctt caaaactcga atggtgaaat catttaatct gtttattttt gtgtcttaaa
    22441 tacacacttg cttatgattt gttttcccaa tgccaaaatc aaccttggac tgcatgttct
    22501 ggagaaacgc tctgataatt accataatat cgaaactctt ttttatccca taggacttaa
    22561 agatatattg gaatgcgttc cggttgataa gacagaacat ccgcttattc aattgagtgg
    22621 aatacctctt gaaggaaatc ccggagaaaa cctggtaata aaagcctata agctcttaca
    22681 aaaagattac cctctcaaag ccgttcaaat ctatttgcat aagtccatac cgtcgggagc
    22741 cggactgggc ggaggttcat cggatgccgc ttttatgctc aaacttttaa acgacatcca
    22801 acacctgggt ttatcaacat ctcagttgga acattatgct gcacaattag gagccgactg
    22861 tgcttttttc atacataaca gacctttgct tgctttcgaa aaaggggatc gttttcaggt
    22921 agtggatttt tctcttaaag gacttttttt ggttctcctg attccaaaaa acatccggat
    22981 ttccacgcgc gaagcctacg cgggaattaa gccggtaaaa cccggctgca agttgaccga
    23041 ttgtatttct aaaccgcctg aatactggaa agattgtttg gtcaacgatt ttgagaaaac
    23101 ggtttttgtc ctgaatcctg aacttctcca tattaaacaa gccctttatg aagcaggagc
    23161 cctctatgct tccatgtcgg gcagcggatc gtctatcttt ggtttgttca gagctgaatg
    23221 tgatttgcag gatgtttttc ccgattgtta ttattggtct gaaaagctct aaccctttaa
    23281 ttctgtaaaa ttatttcatt acctttgtgc cccttagatg taaggatgtt ctaggtatta
    23341 atcttataat ccaatatgtt atggcaaaaa taaagggggc ggtgctggtg gatatcaacc
    23401 gatgcaaagg ttgcgggctc tgtgttgact cctgcccctc ccaagtgctg gcactggcca
    23461 gagaagtgaa cgaaaaagga tataattatg cctatatggc tcagccggat acctgcattg
    23521 gttgtgccaa ttgcgcctat atttgtccgg atggttgcat caaggtctat aaggtaaagc
    23581 tttgattatg aaaggtattc atttgatgaa aggcaacgaa gccattgcac atgcggctat
    23641 acgttgcgga tgtgacggat actttggtta tcctatcact ccacagtctg aaattttaga
    23701 aacactcatg gaggaaaaac cctgggaaag tacgggaatg gtatgtctcc aggccgaaag
    23761 cgaagtagct gccattaata tggtattcgg aggtgcttcc tgcggcaaaa aagtaatgac
    23821 ctcatcgtcc agtcccggtg tcagccttaa acaagaggga gtttcctatt tgtgcggagc
    23881 cgatctgccc tgtctgattg taaatattat gcgcggaggt ccgggccttg gcaccataca
    23941 gcccagccag tcggattatt tccagtcaac caaaggcggt ggacatggtg attatcacct
    24001 gatagtactt gctcccaact ctgtgcagga aatggccgat tttgtgggct taggctttga
    24061 tttatccttt aaataccgta tcccggctct tattttgtcg gacggagtta tcgggcaaat
    24121 gatggaaaaa ctcaagttgc cggccttcaa gcctcgtgcc accgaagaag aaattgccag
    24181 ggattgtccc tgggctctga gcggaagaac cggcatgcgt ccgcaaaaca tcataagttc
    24241 tctggaactg gatccggcta taatggaaaa aaggaatctg gagcgtcacg aacgttatcg
    24301 caaagtggaa gctaccgaag ttcgttacga cacctttatg tgcgacgatg ccgaatttct
    24361 gatcgttgcc tttggttgtg ccgctcggat ttgtcaaaaa tcggtggagc ttttgcgtga
    24421 acagggtata aaagcgggac tgttacgtcc catcacctta tggcctttcc ccaaaaaagc
    24481 catcgaaagc tattgccgtc aggttcgttc aattctgacc gtagaactaa actccggaca
    24541 gatggtcgaa gatgttcgtc tggcagtcaa tggaaaggtg ccggtcggtc attatggccg
    24601 catgggcggc atagtgccta ctcccgaaga aattgtagaa gtactcaaaa ataacagttg
    24661 atatggatct gcaaggctta aaatacactt tgtccattct ggccattatt ttggctattc
    24721 tatctatagt ctgttatttt tccggtcttt ttttctggtt tatcgctttc ggtataaccg
    24781 cagtcctttg taaactggct gaaatcattt tacgtgtacg aaaaaagaaa taatatgaat
    24841 agaagtaata ataattccat tatagcaccc gaaaacctgg tgtacgaaaa atcaaaactc
    24901 ctcaaggata acgtcatgca ctattgtcca ggttgcagtc acggcaccgt gcatcgcatg
    24961 atagctgaag taattgaaga aatgggcatg gaagataagg ccatgggagt gtcaccggtg
    25021 ggttgtgccg ttttcattta taagtacatc aatatagact ttgcagaagc ggctcacggc
    25081 agggcgcctg ccctggccac agctatgaaa cgcctgcgtc ccgacaaact ggtgttcacc
    25141 taccagggag acggcgacct tgctgccatc ggaaccgcag aaaccatcca tgcctgtaac
    25201 aggggcgaaa atattaccat tattttcatc aacaatggaa tttatggaat gaccggcggt
    25261 caaatggccc ctaccactct ggaaggtatg aagacttcta cttgtcccta cggaagaaat
    25321 gtggcattaa acggctatcc cctcaaaatt accaacctgt tggccgaact tgaaggaact
    25381 tgttacgtaa cccggcaaag cgtgcatacg gccgcagcag tgaaaaaaac caaaagagcc
    25441 atccgtaaag cttttgaaaa ctccatggag ggcaaaggca ccagcatagt agaaatcgtt
    25501 tctacctgta attcgggctg gaaaatgagt ccggtacagg ctaaccaatg gatggttgaa
    25561 aatatgattc ctttttaccc cttgggcgac ttaaaagaca aataaaaaat gaaagaagaa
    25621 ataattatat ccggctttgg gggacaaggc gttttgtcca tgggaaaaat cctggcttat
    25681 tcagccctgt tggaaaacaa ggaggtcagc tggatgccgg cctacggccc ggaacaaaga
    25741 ggcggaacag ccaacgttac tgtcattatc agcgacgaac ttattagttc acccatttta
    25801 aattccttcg atacagccat catacttaac cagcagtcga tggataagtt tgaacctaaa
    25861 atcaagcccg gaggggtact tttatacgat cctcacggta ttacccgggc tccggtgcgc
    25921 aaagacatcc gaattcatac cgtagacgct acggaaattg cctccgatat ggataatagt
    25981 aaggctttca atatgatcgt gctgggagct tacttaaaag tcctgcccat tgtgcagctt
    26041 aaatccatct tggaaggttt aagaaagaca ttgcccgcaa ggcaccataa gctcattccc
    26101 atgaacgaac aagccttgca ggcgggaatg gatagagtga agtaaaccaa attgcccggg
    26161 aagccttcaa attcgtctcg ttcagatacg ggatgtgcga ctacgcagac ttagaattta
    26221 taggacaagc ccacaccaag cacttcttta aactgtactc tggccggacc taaaacaccg
    26281 ttggcgtcta cgtatttgat gtcgtcgtcg aaacgcaggg tggattgaaa agtaaccgtg
    26341 agcagttccg taactttaag gttgagcaac acttcccagt tgacgtcaat tttaccgaaa
    26401 ctttgattgt atgcagtaaa caaatccaga ctggatttaa ggtttaccac cttaaaaata
    26461 tcgtaattag ctgaagctct aaagctacta cccaactctg cacggttttt cttaccggct
    26521 tgcaggccgt aatttgtcga aaaagtagta tccgaaacaa aggtcatttt gccggtaacg
    26581 ggcgaataat aagcactaaa atgttcattg ggtttgtaat ctatacccac tgaaagattt
    26641 aggtaagcag gagtcataaa gccggaaacc ctgtcggttg tactgacggc gtaattgtaa
    26701 ccattagcaa actgggtttt aaaatccagt aaggcagcat aataaagggc ttttgaaata
    26761 ctgcgtccgt atttggaggc aaaattgatg ttatcaatat tttttctcca gttgttttcg
    26821 gtggtgtaat acataccaaa attggtactc aaatcgttgg tccacgatac tttgttatga
    26881 acataattta gagaggcatt caaatacaca ttacctgcca ctgagttttc gccaccttca
    26941 ctccaattgg taaaggaagt ttgggaaaaa tttaaaccgg taataccctt gtacgaccag
    27001 gggcttttgg cttcttcggt tttttcctga gcaaacaaag ttatcgaaaa caatgttgct
    27061 attagaagta atctgaattt catatacttt gtgattaacg agtaaatagg ttttttttaa
    27121 cggctgcaaa tgtaagcaaa ataaagctgt gaaaaaatcc ttcatttgtt tttatcctca
    27181 tacaaaattg actagatttg cggcatcaac tggtgatgcc tgtcctttct cgggaccggc
    27241 ataaaaggga atccggtgta aatccggaac agtgcccgct gctgtaagat tcttacaagg
    27301 ctttgtttct gcaaaccact gtctttgtga cgggaaggtg acaaggtgaa tcaagtcaga
    27361 aaacctgccg gttactattt tcaacgcttt cgggaacaaa agctgaaaaa gttttcccag
    27421 ggatcccttt ttcttcttta tgcccggtca cgcacgcggt gtcatcgtgt tgttgaaaat
    27481 cattgtgcaa gcagggcaaa cacaataaaa catttcaggc ttctgtaaac ggatggggtt
    27541 tctgcgtttt atggagtcat tttttgcagg aacccactta ttataatttt aattatgaat
    27601 acaaaacacc ttgtccggtc tttcacatta ggcctgactt tgattggaat gttggcttgt
    27661 gagcctgtgg tcagtaagca tgaagaagaa atctgcatcg attttgagca acctgttatt
    27721 gaaaattttc aaaacaatca ggtttacgcc gccaagggat tggtttttaa caacaagtat
    27781 accctttcgg aatacgaatg ggaaggtaaa acttacactt cagaaacctg gtacggtttc
    27841 tctttgagcg ctatgtgcga tactgttact gagggctttg gcaatcaata cagttgtagt
    27901 gcaggcaaag ctgcaggagg taaacaattc ggcctctttt actacagtgc ctacgatgcc
    27961 gattcttctt gttttgttca gttcgaagaa gaaaaggaat acgtaataaa aggcatgcaa
    28021 atcacaaatt caacctatgc ctacttgtcg atgaaaaatg gcgatgattt tgcaaagaaa
    28081 tttgaagcag gtgattatct gcgaattata tttaaatcat acgatgccga gggaaaatat
    28141 ttagctcaaa aaacctttta tttggccgac ttcagagaga acaaaaaaac cttattgaat
    28201 tcctgggaat ttgtcgatct gagagaattg ggcaaactaa ataaactcag tatatttttt
    28261 gagtcgagcg acaccggcat gtacggcatc aatacaccga cctatgtctg tgttgacaat
    28321 ttgacttaca taaaagtaca agaataactg tctgccggca aacttgtact gctccgcaga
    28381 agttgctttg gctgtcaaag tataaaacaa gactccattt agcttatgcg gagcattttc
    28441 gtatctttgc gccaaaatac gaccatgtta cgcattgata ttattacggt tttacctgag
    28501 cttttggaag ggcccttaag ctgttccata atacaaagag ccagagataa gaaactggtt
    28561 gacatcaatg tacattgttt acgtgattat tccaccgatc taaa
//
LOCUS       DAOSTD010000017        28432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1834_length_28432_cov_12.301371, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000017 DAOSTD010000000
VERSION     DAOSTD010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 28432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28432
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1834_length_28432_cov_12.301371"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..316)
                     /gene="atpE"
                     /locus_tag="PLI37_02050"
     CDS             complement(74..316)
                     /gene="atpE"
                     /locus_tag="PLI37_02050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOQ57833.1"
                     /translation="MLLSILLQVATDIAILGKALAVALTVFAAGFGISKIGASAMEGI
                     ARQPEAGGDIRISMIIVAALIEGVALFAIVVAGFIL"
     gene            complement(366..1472)
                     /gene="atpB"
                     /locus_tag="PLI37_02055"
     CDS             complement(366..1472)
                     /gene="atpB"
                     /locus_tag="PLI37_02055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOQ57834.1"
                     /translation="MFSKALKYFLSAWMFLAMNTSLSAGVVSQADSPEAEAAPLDVKE
                     LILEHLKDDYEWHIATYKDKDISIPLPVILFSKNSGVQLFSSAKFHQNKGYYKGFYIS
                     KSGKYKGKIVETDASGNEIRPIDLSLTKNALSLLIGSFLLILLMLFIARTYKNEPIKP
                     KKGVVGAMEVFILSVYDDVIKPVLGEDYKEYRYYLLTLFFFIFFNNILGLVPIFPGGA
                     NVTGNISVTFTLALCTFLIVNLTGTKEYYKSIFWPDVPVWLKFPLPLMIIIELVGLIT
                     KPFALMIRLFANIMAGHAIVLGLVSLIFITAGMGAALSSSMTGFSVVLTIFIYFIEIL
                     VAYIQAYVFTTFTAVFISGARVKSPKTEKSLKST"
     gene            complement(1462..1872)
                     /locus_tag="PLI37_02060"
     CDS             complement(1462..1872)
                     /locus_tag="PLI37_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57835.1"
                     /translation="MKQLRKKLMLCLTATLAGVGGILCFLVVVLFKHDITTCYVSIPI
                     FFLFVGVMSILTITKNGITYKNNGRKRANKYMLVRVIKIFLGAAFFLLYWLLVKPEDV
                     KGFALTFAVFYLTYLAFETWSFIQVEKKIKNNVQ"
     gene            complement(1869..2120)
                     /locus_tag="PLI37_02065"
     CDS             complement(1869..2120)
                     /locus_tag="PLI37_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57836.1"
                     /translation="MYLEIITVEQKLFSGNVESVTVPGTMGPFTILKNHAPIVSSLTK
                     GTVKYRSNQAENKLNISGGFIKANLGVVTICLTSSQHVR"
     gene            complement(2148..3677)
                     /gene="atpD"
                     /locus_tag="PLI37_02070"
     CDS             complement(2148..3677)
                     /gene="atpD"
                     /locus_tag="PLI37_02070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOQ57837.1"
                     /translation="MIKSIGTISQIIGPVVDVYFDLDASKQEELPAIHDALLIKRKNG
                     QTLYAEVQQHIGENTVRAVAMDSTDGLRRGMTVRTHGGTITVPVGEQVKGRLMNVTGD
                     PIDGMQDLNREGAYSIHRPAPKFKDLSIITEVFFTGIKVIDLLGPYAKGGKIGLFGGA
                     GVGKTVLIQELINNVAKAHSGYSVFAGVGERTREGNDLLREMIESGVIRYGEEFKKSM
                     EEGDWDLSKVDYEELKKSQATLLFGQMNEPPGARASAALSGLTVAESFRDGTDTNKRG
                     GNDILFFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLSTEMGAMQERITSTKYGSIT
                     SVQAIYVPADDLTDPAPATTFSHLDATTVLSRKIFELGIYPSVDPLESSSRILSPEIL
                     GEDHYQTAQRVKQILQRNKELQDIISILGMEELSDEDKITVRRARKVQRFLSQPFFMA
                     EQFTGVPGVMVPIEETIRGFKMILDGELDEYPEMAFLNVGTIDEAIEKGKILLKQTDQ
                     Q"
     gene            4131..6791
                     /locus_tag="PLI37_02075"
     CDS             4131..6791
                     /locus_tag="PLI37_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOQ57838.1"
                     /translation="MKRFYPVIITFLILALPIQARKSWDVREKGISAASVEKILADTE
                     NWKQTIKDGVEDTRATNLNAFTGEVKAQGEILPEFSELYFANVQANTFEVRWQEGNAY
                     FRMNKDVQTFTIKKLVAGQTITIITKSANSSDPRGIECKSGHCTRTAGAEKMTGAEGD
                     NVNVFQVNADITDSVDVAFGPNVGGLLIYSVIVDQGDEEFDFSESKVAYLYDASTQDI
                     DSDPFFNYTKIAEVASESIDIKDFSSVDTDTLSALENYDLVVLSYGLKADHPFVAMLK
                     KEVNRVPILNLNASLNEAWGWATLRNPSNAAEEGGIGTIKIPEGAEAHAIYADIDVSS
                     AEIALFFESDPALLKCNLIQAYQAVEGGLFANDSVLAVVEGPAGTFNAIHEHASANTY
                     MLIPFGPDAVVVEGESNLSDDAISLINNAVNYLIESKATVVAVMTPSISSDYRDELTI
                     VSIKCATPDATVYYTTDGSEPTENSPIYTAPFQVTEDSTLVKAFAVKQGYDDSKTAAA
                     TILVKKVAKTPVIVLEAQEGKTVVSITCATEDATVYYNYTGSKSIAASAVYTAPIEVR
                     YPLTITAFASGGTYIDSDITSKEIGVTGITKDNIRLDTLAHFDANSTDWYWETEGGSS
                     KVAYYVGKNAKSAYASIDTIVEGNDTTYNYHFVDDVFIYAQNDSLNAKDNGWFMRTKG
                     QVLQWENTNPLMEVGYAGATGYYADAVEDMITTPTRYHITFGPKKSGEPFTASIETSQ
                     KHQGPFDIITFAGNNNSDGASMTLVLETSLDGSDWTTIDTLKHSYYRRFWKRNVSHYE
                     GSDQVFVRVTQVSGSTKAVVYDILLMNNGEVSQGYVALPVHRQAAEVIATEYYNLNGI
                     RMAGPAYQGITIVKKIYSDGHIKVEKIFIK"
     gene            6826..9663
                     /locus_tag="PLI37_02080"
     CDS             6826..9663
                     /locus_tag="PLI37_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOQ57839.1"
                     /translation="MKKILVFLMAATLCFGTSYAAKKRVAFIYQEGYNSGKAVDYGLK
                     EGGYTPDQDPIHNALAAEFDLTLYNSPSTVEVDYDELATYDCVVFSEAMPGNSTLTNG
                     LVKLVGRVPVVSMKTYNYTNGRWSWAAPSNPGTRCNTININEGFDSHAIFAGLTRESD
                     GSIALYDPAKPASSNRLQGFATSGIIAGSPIDAERDKLLAVTTSDNYNNVHEITLLGK
                     PYVLVGLSSDNIWAINDNGVRIIINAVKYVMGEGDIVMEEHEIAYIYDESATGYCGID
                     NDPVYNGTKIAEINTTAINIKDFTAADTDTLAALENFDAVVLCGALNPEHAFVEMLGT
                     EVNRVPMLNFNAFLSQAWGFGTGVNPTANASEQGGIAQINVDSAYLDDALFTDMIIEE
                     GGAVVLYHNYDASLIKTNLVQGFTATDEQLFSKGEVLATVVSGEDTYNALYRLGNKNT
                     YILLPMSFESIFIEGESNLSDDAVQLINNAIDAIISSKGSVTSCIIPSCTFEYSNLST
                     QVTLSSVTEDAVIYYTLDGTEPTAESLRYKEPFTVTVDSTVIKAIATKQGFNNSGVYS
                     GIILVKAVAEAPVITLEAQEGKTVVSITCPTEDATIYYNYTGSNIIAASAAYTAPVEV
                     RYPLTITAFAGGGSYISSGIVSKEIGVTGITKDNIRLDTLAYFDANNTDWFWETSGGS
                     SKVAYYVGKNSRSSYASIDTIVEGNDTTYNYHFIDDIFIYAQNDSLNAKDNGWFMRTK
                     GQVIQWENTSPLMEVGYAGATGYYADAVEDLITTPTRNHITFGPKVSGEPFTASIETS
                     QKHQGPFDIITFAGNNNSDGANLVLVLETSLDGVEWTTIDTLGISYYRRFWKRNVSHY
                     EGSDEVFVRITQVAGGTKAVVYDILLMNNGEVSQGYVALPVHRQAAEVVATEYYNLNG
                     IRMAGPAYQGITIVKKIYSDGYIKVEKIFIK"
     gene            9758..13324
                     /locus_tag="PLI37_02085"
     CDS             9758..13324
                     /locus_tag="PLI37_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOQ57840.1"
                     /translation="MKRLASFFVFTIWAMTVTAADALPAFPGAEGFGKYAVGGRYGSV
                     YRVTNLNDSGTGSLRDAVSTANRIIIFDVSGVIKLNSRLVFKNNLYVAGQTAPGEGIT
                     VYGNGVSFSGASNTIVRYLRFRMGSGGDAGKDAAGVANGTNMIFDHLSVSWGLDETFS
                     ISSDGKGALGDITIQNSIMSQGLMTHSAGGLMQADHITLYRNLYVDNSTRNNKIKGTN
                     QYVNNIVYNWKNGCYIMGGDSQGTSYCNATNNLFINGPAVGGNAFTGGNSNFNIYADD
                     NWQDKNRDGILNPYSIPQSEYSGPPTFQSQPYDYPALPAYPGNALIDSLLPTVGASLP
                     YRDPVDFYVINETMSFGKEGALISKEAELPIGIPTSWPHQSFAKPVDTDGDGMPDAWE
                     TANGTDPTVNDAMTIAANGYANIENYINSITIDDRAFFLRRPFVLSLQESSQNQLVIN
                     WFDYTEGEDGFIVEQLIDGNYTEVARILAADYKLRGTDGSAPGSAAGERTGSVACTVK
                     NLMPATAYSFRVKAFKTEGNHFSEYSSVLEAKTQPEYVPMVEWDSYEPDLIWNIASGA
                     WNFTDLNWGENVYTDSTAVLLTPSESATITIDSVVSPKTVVVGQDHDLTLAGAAIAGE
                     GTSVNKFGKGKLDMGSAAHTYTGATALHSGVLRFATLKNGGENSSIGASQEFAQNWIW
                     DGGCWEYTGGNTSTNRSAKVYQNTTFNISSGATVTMTGTLEGTGGVTVKGGQISVGST
                     SFFGYEGPTRLEEGGSIYLSTTEISKVGLGKSSQLILAGGKLSTKGESNNYETYSFPI
                     HAESGTYSYFAPNRNCSINNVVTGDGTIEYQIPYVREYICKSWDGFTGKLIARGMSSS
                     SDGSQLMLYNGIGIPNAVVELRGNTRVICWKAAATYYLGGLSGASGTYLSGASKNTRN
                     TTMTWIVGGANTDETFWGVIDNRCSAANYDCTTHIQKVGTGDWRLAGTSIYSGSTTVN
                     GGRLVVNGSLTGNGAIAVNNGGTLCGKGSVKGRVTVNNGGKVAVGDTLFNKSDVFTLN
                     GGLNLLNGGIIQVPLYRKQILNNAARIKFAANSTLNGTLELDMTDVTIDIPNNSSFTI
                     FTMGTGVTLSGSISAVEPAEPSETQEWDLSELFTTGKIYVREKGYTALPQVSVVPVSE
                     QYFSLEGVPLGMEPPYTGSPALFIVKKKYQDGSVRIEKVMVY"
     gene            13350..15659
                     /locus_tag="PLI37_02090"
     CDS             13350..15659
                     /locus_tag="PLI37_02090"
                     /inference="COORDINATES: protein motif:HMM:NF027701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin"
                     /protein_id="HOQ57841.1"
                     /translation="MRKRIFFFSLTGICLLLTVLTSCQEELANRPHYAPPDWLKGSAY
                     EVLQDEGNYTMFLRGVDLAGYAPIVKGKSILTVMAPNDSAFTAYLQKAGYAGIDQMHA
                     ENPEQLSKLIGFHLLYYAFNWDNLVNFRPIEGDEASAEALLVNAGCYYKFRTKSADPI
                     SMEWDGKDSVRVYHFERFIPVFNTQLFATRGIDPKYNYEYFFPNTEWTGSHHTGKIDG
                     FNVSNASVIDDDHVITDNGYLYHISQVLDPLETIYTELKNNADYSIFFELFNSYGEYQ
                     EEPELTTDFGNGQTLYLHTHPGLVNIACEWPVTDYTAMDRLSSVGYNLFAPSNDAVQN
                     FFTTYWTPGCGYSNLNELDPLILQYFISQTFGSDDVVAFPEEIKKGLIKTVYGTPVNI
                     DPDQVTWRQMCCNGALYGMDRMTPPAIFSSVIGSAFRDKRFLPYLYALDGSDLVLALA
                     SQNTEFVALIPDTAQFRGAAIRFETIAGGRHELQVIGDDGNYAAMSVGTMRDMVNMNV
                     SSSVNQLSTTGFQVVETNTAFNYWYLYDGKITTSALFNKQLNPTYVDEIYFDFAPLEM
                     NPAQPNGGKWDNGSVYTYTAPSIFMPGEDEGLNRTLAVCNDNTYPYYLFSQLLSKAGL
                     VEEQQLKIVMDDARFIAFVPTNEAIKARITEIPGCDKCSVSDDMVLIGNPNKALLANY
                     LRSYFIISDLTPFSVYPYPGSGMKGSFDTSGAYKLNITDTGTTLTVSLTDKGGIKGAE
                     VSLYNTEKFHSLPFSYSDGAFHLINDILL"
     gene            15670..>18416
                     /locus_tag="PLI37_02095"
     CDS             15670..>18416
                     /locus_tag="PLI37_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOQ57842.1"
                     /translation="MKQIKIFLIVLVAVLVWPQLQAQNTVVTGTVTELYDGKVEPVYN
                     ANVVVVSSNNRYITGAITNIDGQYSIVVPAYEKEPRIRFSYIGLKTQTIDYTGQKVLN
                     VRMEPDSKSLDEVQVTGRRVDRDEMGISFTEQTSSTQKIEMSDIVQSTPISDITEALQ
                     GQLAGVDIVVGGDPGARSAIRIRGVNSLNASSEPLIVVNGIPYHVDIADDFNFATANE
                     EDFGALLNIAPYDIESIEVLKDASATAIYGTKGANGVLLITTKRGSKGKTTFGFNSKF
                     TTKFEPDPIPLLDGNQYVALMQDAIWNAANAKGLTSARNELEQLFNTYDINYVRDWQY
                     FDEYNVNTKWLDYVRQNAYTTDNNFSLSGGGDKATYRYSLGYLNETGTTVGTALNRFN
                     TSFNIRYDFSERLMVNTEFTYTQTNRDANALSTLRSEAQRKMPNKSPYYIDDFTGEAL
                     PSYFSRQNEDFQGAFNGSYHYNPVAMAEMGFNRTMQREEKMTLNVQYVFPFSLMYKGY
                     VSMNMRTSKNRKFIPQEATGVLWTSNYANQSTDAFSDRMTLQTEHKLLYNKTFRDVHA
                     VVATVLANMSQNDSYSYTSATSGNASPNLSDPVAGSVVSSIGSGFSQVRSVRAVAQGV
                     YTYDRRYVFRTTVNREGNSAMGKSERFGTFPAFGVAWNIHEEPFIRDRDNPWLTEGKL
                     RVGLGWSGNAPSGASMYLGAYQSLGEYMSMPAIYPVRIQLDKLKWETSREWNYGFDLE
                     FFRKLGFTFDHYNKYTSDLLLGNVSIPSSTSYTSIRYFNSGELSNKGYEFRITYNAIN
                     TTDWKLSFNFNISHNENKVEKLPANMKEENYTFGNGNYAVRVIEGDPIGSFYGYRYLG
                     VYDNKEETYALDEEGRVMHDYKGRPIVMKNGSTTVFPGDARYQDINYDGVINEYDIVY
                     LG"
     assembly_gap    18417..18516
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18615..20201
                     /locus_tag="PLI37_02100"
     CDS             18615..20201
                     /locus_tag="PLI37_02100"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ57843.1"
                     /translation="MNRFLLTTMTLLALTLSSCNEWLNILPNNEQVTDNYWKTRQDVE
                     AVVASGYYYMRQAVPTMLVWGEVRGGALYSSNSQHSYLQNFNLTPSSGICDYASLYKV
                     IGMANSVLKYAPSVTTEDETYYESVMKSHLCEAYFQRAYCYLVLVKNFREVPLVVEAF
                     VNDDADYMTPKSSEADIIAQIKDDIEKALATGAAKDIYEDTDGYTWQTKGRATKWALY
                     ALMADACLWNHDYDEAIQYCNYILDAPEGDTSFRPRFMSDMTKWYEIFYPGNSNESIF
                     ELNWETHMSQTNNFGSWFSVGASSTLKVTDHLKAKLIAETDEVMANSIRNGWATDGRV
                     GRMIFGTFVSDAADQTKYGTANYLSVWKYMGTDIADRGNTRPAQDANFILYRVPDVML
                     TKAEALVMKGGAQSWQTALDLVNRIRQRAGLDSVNINVSETDEMVMLDAVMNEWTMEF
                     LAEGKHWYNLLRMGRYDKGDGVYKERFINEIIESNQTTKDEWIKSVLMDENAWYMPIP
                     YNEISVNENLVQNPYYKTTK"
     gene            20210..21880
                     /locus_tag="PLI37_02105"
     CDS             20210..21880
                     /locus_tag="PLI37_02105"
                     /inference="COORDINATES: protein motif:HMM:NF028443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5108 domain-containing protein"
                     /protein_id="HOQ57844.1"
                     /translation="MKQTSIKIFMSLAVALLLAACQDDKLNSVYQIFDDNPASKTLEK
                     NENHSEWVKILRYSDMYNALNQATQAFTLLVPDNDAVFAFYQQRGVSGIEDLGKDYAK
                     ALILHHTMLDSIMVESMQLKNSLNNLNGDAISVAIDTLNPGQFIFGGLARTTESGVHA
                     YNALMYYIDAVLTPLVESVYDKLVEDGRYGIMKAALDATAWTQQLHTIYDTVPQEGGG
                     YVVNRRAYALLAVSDEGFRRNNIQSFNDLVAKLSAGTDYTEPDNALNEYVAYHILTNN
                     YTLADLKEMSGDTSRIWETSAKNQVFMITVDTTAGDWQFNILDSAILPTRFVEEYCDV
                     LCKNGYLHEIDAYLPVWEPKPSIVVWDLANYIEVKNTVIESAGPEEYQPAEPVSSEKK
                     IDITKCGAYDYVVSESGVGGSSYAYITYVTCKNNLRDCLKYDRVVFNLGYMGSVSMKT
                     PTLVRGKYKVELNFVYLSDHNFMRQMTDGNGGMIRMTIDGENLKSVTPYTTVPRNVAK
                     VYTATLYDEVEFEQTSTHDFKFVVMDPAASTNSRFSLQFDCITFTPIE"
     gene            21887..>22542
                     /locus_tag="PLI37_02110"
     CDS             21887..>22542
                     /locus_tag="PLI37_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOQ57845.1"
                     /translation="MKTINRICLTGCMLLCLTACDENWQRHYYQSETTITNEQLIMVS
                     QTTQEYLSGNDSRHLSDMYQFLQDNKVFNMLQKKGQLHTLLMVENDAFKAPEAERAEY
                     IAHSHVTDICLSPSNLYHGERVLMWHQKFVTVGIDSLGLLGDLTHITFNGSPVKEVVK
                     TTDGYIYVLDAMIETPISLQDYINDLPEEYSLFKDMVLASGGKLFDKNNSKPIGIDAT
                     G"
     assembly_gap    22543..22642
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22643..23398
                     /locus_tag="PLI37_02115"
     CDS             <22643..23398
                     /locus_tag="PLI37_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57846.1"
                     /translation="QSLDLDNPIKVSNGIVYEVKKLRFPNNILIYRIKDFYRNYEYCT
                     AEEKELYFKYDNLKNIEMKSEVEAWTPMPGVWPMHGNVTFSCYLTDASQGFYLDMQLI
                     KPVYDEAGNISSVRPYLIPPGSYRFAMGFFQNMGVTMNVSVIVLNGSERVKLGEASVT
                     LASTTEFHYDRGTFLSDTWPEGYKEVKNDYTHSKKNNYNTDGGAVMAELEIPDLKGDG
                     SALPMIIKLEMSTGKDSRLAFHHWCLRPTSDNY"
     gene            23410..26571
                     /locus_tag="PLI37_02120"
     CDS             23410..26571
                     /locus_tag="PLI37_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOQ57847.1"
                     /translation="MKQFYTIITALMLALPTLAQAVEGIVISSDGQAVRGAVLSSPGQ
                     TTVVTGEDGTFRMDLQKDAVLSVWADGYYYQQIRYKGQSSLKVVLARQDAHKYTESKV
                     LPFRVEQNVNLPEVNTLSKKDFALGARSVEQALQGELAGLQLINKSGMTGEGAFLSHH
                     GIQTLAANASPLVVINGVPFMPSMDESQLIGGFSKSIFQAYNIYDIQDISFLSGAEAS
                     VYGSLGANGVLLIETDGASSDDMDTKISYHGKFGYNWNDRRLPMMKSSQYKSYLSDIG
                     MTYYNNMETFFANFPFLSPDERDAYNYLYRFDTDWQNELFRRGVTTDHLVRVEGGDAI
                     AKYDISLGYQHDEGALKNTQSDRFNAQINSNVIVSKKFEIGTSIGLAFLNGSYQEQGL
                     IMETNPLLAAYRRAPVLSPYKNDMAGNLLETYASYYYGNNTNTDFIVSNPLAIVNTLA
                     ADKRQYDININARVLYKPTNQLSFMALLGLYYNYDQEGIFVPGINNKDVVPQFDTYGE
                     SKNIVRQGVNETFNSFYHLNAAYNNVFDDVHGVDLRLGGQIISNSFETDMGVGRNTAN
                     DFYQTLADVQRVGRFFSGYGAKWSWLNAYLQAAYTYSDLFRVAANVSTDGASSTGVNT
                     TRMGVFPSASATVMLANFDFIKSAEAVNRLNLYADYGKTGNSRFSHTYGSYYYVSRLY
                     QGIAGIVRNNVPNTEIKWEENLKANMGIDMALWRHRVQIKLGYYNNRATDVLMAGSES
                     ALYGTGVHYVNDAAIDANGFDLSLNVSPVCTGNFKWILGGNITQLNNKVTSLGNRTHI
                     INKLSDGAAIITKVGESPYSFYGYRTDGDYPVYSTVKEAQSAYKYTENGVEYSRPMRN
                     RNGVEFQAGDIKYVDQNNDGIINDLDRVLLGSATPDFYGGLFNRFQYKGFAIDLNFVY
                     SLGNEAYNAVRRITESSLDMSNQSLAVLRRWTVDGQITDMPRANYYDLVGNNDFSDRW
                     IEDASYLKLRDITFSYTFDNILLGFIQGGTLYVSGQNLWCLTPYLGNDPEFSYSYSAM
                     MQGVDYAKLAAPKTVTFGVNLKF"
     gene            26591..28366
                     /locus_tag="PLI37_02125"
     CDS             26591..28366
                     /locus_tag="PLI37_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57848.1"
                     /translation="MKQNMIYRIFAASLLVLSMTACSGFFESKTDDVLSADDYISSQT
                     EVYTGYLGIITKLQAVGDKAIYLTDTRGELLEPTENSLPELIALYNYEQDLTGNGYAS
                     PDAYYEVVIACNDYLENMRAFRKARPELVNDERFDALQSLTLRVKVWAYLTLAEIYGQ
                     AVWFDDPIVKIQDLTDTGRFQLMNIDQVVDRCIDFLDNGDASTDYINGKLDFSWYRWL
                     DPETALASSQYRYWDYMTPPFEGLMAKLCLWKGAALQGKGDYDGARPYYQRCADLILE
                     ALNEVISVQSNAGNGYYLPTAYVPGRYARFWDNVEPYATEVICAIIYDYTRNQTNKLL
                     YHFSSEYPNQYLLRPNVAMVEERFETDDNNPGFSTSVEARNRVVLGDREGKPYLAKYR
                     PIGSARRANAYQDDVHIYLFRCFEYHFFLVEALNNLGRFEAADAVFNGGLAPTDFLTA
                     ADGSMIKDSDGYGTPKPGFEGFINDWTSVASWGTRKYSHMGIRGCFSLKDRPFAIEYY
                     TEAGRRANMKANDMAILDEAMLEFAGEGKVYPMLIRMAKRWGNGTYDATIISSRVAPK
                     YGTRAAEMKPKIESQFFVPWDLLLE"
BASE COUNT         7590 a         6588 c         6747 g         7307 t
ORIGIN      
        1 acgactattc caaatgcgat taacatccag aacaccaatc ccaaatcggg tgtaagtaac
       61 gacatggcaa cgattataaa atgaatccgg caacaacgat ggcgaaaagc gctacacctt
      121 cgatcaacgc ggcaacaatg atcatactga tccggatatc accccccgct tcgggctgac
      181 gagcaatccc ctccatagcg gaggctccga ttttactgat accgaatccg gctgcaaaaa
      241 ctgttaaggc aacagcaaga gcttttccta aaatagcgat gtcggtggca acttgtaata
      301 aaatgcttag taacataatt gtttgtttta aaatttatct gttaataatt cgttgaattt
      361 agtatttaag ttgatttgag agacttttcg gtttttggtg atttaaccct tgctccactg
      421 atgaatacag ctgtgaaagt ggtaaacaca taagcttgga tatacgccac caaaatctct
      481 atgaaataga tgaatatggt taataccact gaaaaacccg tcatggatga actgagtgca
      541 gctcccatac ctgccgttat aaatatcaaa cttaccaaac ccaatacgat ggcatgtcct
      601 gccatgatat tggcaaaaag acgaatcatc aatgcgaagg gtttggtaat caagccaacc
      661 agctcaatga taatcatcaa aggcaaaggg aatttcagcc aaaccggtac atcgggccaa
      721 aaaatgcttt tgtaatattc tttggttccc gtcaggttta caatcagaaa agtgcacaaa
      781 gccaaggtaa acgtaacgga tatgtttccg gtaacgtttg cccctcccgg gaaaataggt
      841 actaatccca aaatattatt gaagaaaata aagaaaaaca gggtcagcag ataatacctg
      901 tattccttgt aatcttctcc taaaaccggt ttgatcacat cgtcgtaaac agataatata
      961 aaaacttcca tggctcctac cacgcctttt ttaggtttga taggttcatt tttgtaggta
     1021 cgggcaatga acagcatgag gagaattagc agaaaggaac ctatgagtaa agaaagggca
     1081 ttcttggtca gtgatagatc aatcggtcgg atttcgttgc cgcttgcgtc agtctccact
     1141 attttccctt tgtatttccc gcttttgctg atataaaaac ccttgtaata acccttgttt
     1201 tgatgaaact tagccgaaga aaacagttgt actcccgaat ttttactgaa caatataacg
     1261 ggtaggggga tgctgatgtc tttatcttta taagtggcta tatgccactc gtaatcgtct
     1321 tttaaatgtt caagtatcaa ctctttaaca tccaatggtg ctgcttcagc ctccgggctg
     1381 tcagcctgcg atacgacccc ggccgacaaa gaagtgttca ttgccagaaa catccatgcg
     1441 gataaaaaat atttaagagc tttactgaac attgttttta atttttttct ctacctgtat
     1501 aaaactccac gtttcaaaag caagatatgt caaataaaac acagcaaaag tcagggcgaa
     1561 gccttttaca tcttcgggtt taactaaaag ccaatacaat aggaaaaaag cagctcccaa
     1621 gaaaattttg ataacgcgca ccaacatgta tttgttggct ctctttctgc cgttgttttt
     1681 atatgtgatg ccgtttttag taatcgtcag aatggacatt actccgacaa ataagaaaaa
     1741 tatgggtata gaaacgtaac aagttgtgat gtcgtgttta aagagaacaa ccaccaaaaa
     1801 acacaagatg ccgccgacac cggccagggt ggcagtcaga cagagcatga gtttttttct
     1861 gagttgtttc atcgcacgtg ttgggaagaa gttaaacaaa tggtgactac accgagattt
     1921 gctttaataa atccaccgga aatattcaat ttgttttcag cttgattgct cctgtattta
     1981 acagtaccct tcgtcaggct cgatactatg ggggcatgat ttttcagaat ggtaaacggt
     2041 cccatagttc ccggaactgt cacagattcg acatttccgg aaaacaactt ctgctctact
     2101 gttataattt caagatacat aaaactcctg ctgaaattct tgagatatta ttgttgatcc
     2161 gtttgcttta gcaaaatttt gcctttttca atggcttcat ctatggtccc gacatttaaa
     2221 aatgccatct ccggatattc gtccaattcg ccgtccagga tcattttaaa gccgcgtatg
     2281 gtttcttcga tgggtaccat cactccgggc acacccgtaa attgttcagc cataaagaag
     2341 ggttgcgaca gaaatcgttg tactttccgg gcacgtctga ccgtaatctt gtcctcgtcc
     2401 gacaactctt ccatgcccag gatggaaata atatcctgta attccttgtt tctctggagt
     2461 atttgtttca cgcgttgagc cgtttggtaa tgatcttctc ccaaaatttc agggctcaaa
     2521 atgcgggacg acgattcgag gggatccacc gaggggtaaa tacccaattc gaatatttta
     2581 cggctgagga cggtggtggc atccagatgt gagaacgtgg ttgccggcgc cgggtccgtc
     2641 aagtcgtcgg caggtacata aatggcctgt accgaagtga tggaaccgta tttggtggag
     2701 gtaatgcgtt cctgcatggc tcccatttcc gtggaaaggg taggctgata acctacggca
     2761 gagggcatgc gtcccaacag ggccgaaact tctgaacctg cttgtgtgaa gcggaaaata
     2821 ttatcgataa agaaaaggat atcgtttcca cctctcttgt ttgtgtctgt tccgtcgcgg
     2881 aacgattcgg caaccgtcaa gcccgacagg gctgctgagg ctctggctcc gggtggttcg
     2941 ttcatctgtc cgaacaatag ggtggcttgg gatttcttca gttcctcgta atccactttc
     3001 gataaatccc agtcgccctc ttccatactt tttttgaatt cttcgccata gcggatgaca
     3061 ccggattcga tcatctccct gagcaaatca ttgccttccc tggtgcgttc tcctacgccg
     3121 gcaaaaaccg aataaccgct gtgtgctttg gctacgttat taattagctc ctgaatcagt
     3181 accgtttttc cgactccggc tccgccgaaa agtccgattt ttcccccctt ggcgtaagga
     3241 cccagcaagt cgatgacttt aatgccggtg aagaaaactt ccgttattat ggataaatct
     3301 ttaaacttag gggccggcct gtggatggag taggcacctt cacgattcaa atcctgcatg
     3361 ccgtctatag gatctccggt gacgttcatc agtctgcctt tgacctgttc gcccacgggt
     3421 acggtgatgg tcccgccatg cgtacgtacg gtcatgcctc ttcgtaaacc gtcggttgaa
     3481 tccatggcga ccgcccgcac ggtgttttct cctatatgct gttgaacttc agcgtataaa
     3541 gtctgaccgt ttttacgctt tatcagcaaa gcatcgtgaa tagccggcag ttcctcttgt
     3601 ttacttgcat ccaggtcgaa ataaacgtcc actaccggac caataatttg cgaaatagtt
     3661 cctattgatt taatcatact caaactgtgt cctaatataa gagggggcaa tagcccacaa
     3721 aattacaaaa aaacagaagc tttgaaatta tttagaacta attttattat aaagttgggt
     3781 tgcagggtca gctaccgctg taagtcatgc tccggttttg tcaaagtttc caaacaaaaa
     3841 ttcaaaattc tgtttaatcc gtcttatttt tgtggaacat tttccaatat tttactaact
     3901 tgcggctctc taaagtgcac agtggtcgtt agtttgaaat aaaagacagg gaaaacgcgc
     3961 tttgcacact gttgggggac agaacctttt taaaacctaa tgagttgtaa aacagtatta
     4021 tcagtagcac taatagcttt catatcggta gctgttacgt cagtttttcc ggtgtcaagg
     4081 actatctttt ttgaataaca attgaataac aatactaaat actaatgctt atgaaacgtt
     4141 tttacccggt tatcattaca tttttaatac tggcattgcc tatacaggcc cgcaagtcgt
     4201 gggatgtgag agaaaaaggg ataagcgcgg cttccgtgga gaaaatcctg gcagacaccg
     4261 aaaattggaa acaaaccatc aaagatggag tggaagacac acgtgctacc aacctgaacg
     4321 cttttacggg agaagtcaaa gcgcaaggcg agatcttgcc cgaattcagt gagctctatt
     4381 ttgccaatgt ccaggccaat acttttgaag tccgttggca agaaggcaat gcttatttca
     4441 gaatgaataa ggatgttcag actttcacca taaaaaaatt ggtggcaggt cagaccatca
     4501 ccatcattac caaatctgcc aacagtagcg accctcgcgg catcgagtgt aaatcgggcc
     4561 actgtacgcg tacggcgggt gccgaaaaaa tgacgggagc agaaggcgat aacgtcaatg
     4621 tctttcaggt gaatgcagat atcaccgact ctgtggacgt tgcttttggt cccaatgtgg
     4681 gcggtctgct tatctacagt gtcattgtgg accaaggcga cgaagaattt gatttcagcg
     4741 aatccaaagt agcttatttg tacgatgcat cgacccagga catcgacagc gatcccttct
     4801 ttaattacac caaaatagcg gaagtggctt ccgagtccat cgacattaaa gactttagct
     4861 cagtcgacac cgatacgctg tctgctttgg aaaactacga tttggtggtg ctttcttacg
     4921 gactgaaggc cgatcatcct tttgtggcca tgttgaagaa agaagtgaat cgtgtgccta
     4981 ttcttaacct gaatgcttcg cttaatgagg cttggggatg ggctaccctg aggaatccga
     5041 gcaatgcggc cgaagagggc ggtatcggca caattaaaat ccctgagggc gccgaagcac
     5101 acgccatata tgccgatatc gatgtcagca gcgcagagat cgctctgttc ttcgagagcg
     5161 accccgcttt gcttaaatgt aatttgatac aggcttacca ggccgtcgag ggcggattat
     5221 tcgccaacga ttccgtgctg gcggtagtag aagggccggc gggaactttt aatgccattc
     5281 acgaacatgc ttctgccaat acctatatgt tgattccttt tggcccggat gcagtggtgg
     5341 tagagggcga aagcaatttg tcggacgatg ctatatcgtt gattaataat gcagtgaatt
     5401 atctgattga aagcaaagct acggtggtgg ccgtaatgac gccttccatt tcgagcgact
     5461 atcgagacga gctaaccata gtcagcatta agtgtgccac tcccgatgcc acggtttact
     5521 acaccaccga cggcagcgaa cccaccgaaa acagcccgat ttacaccgct ccattccagg
     5581 tgacggaaga ttctacttta gtcaaagctt ttgcggtgaa acaaggttac gatgatagta
     5641 aaaccgccgc tgcgacgatt ttggtcaaga aagtggccaa gacacctgtc atcgtcttgg
     5701 aagcacaaga gggtaaaacc gtggtaagca tcacctgtgc taccgaagac gctaccgttt
     5761 actataatta caccggcagt aaaagtatag cagcgagtgc tgtatataca gctccgatag
     5821 aagtgcgtta tcccttgacc ataaccgctt ttgcatcggg tggtacctat atcgactccg
     5881 acattacttc caaggaaatc ggcgtaaccg gtattaccaa agacaatatc cgtttggaca
     5941 ccctggccca cttcgatgcc aacagtaccg actggtattg ggaaaccgaa ggcggctcgt
     6001 ccaaagtagc ctattatgtg ggtaaaaatg cgaaatccgc ttatgcatct atcgacacca
     6061 ttgtggaggg aaacgatacc acctacaact atcattttgt agatgacgtc tttatctatg
     6121 cgcaaaacga tagtttgaac gccaaagaca atggttggtt catgagaacg aaaggtcagg
     6181 tgcttcagtg ggaaaacact aaccccctga tggaggtggg ttatgccggt gctaccggtt
     6241 attacgccga tgccgttgaa gacatgatta ccactcccac caggtaccac atcacctttg
     6301 gtcctaagaa atcgggcgaa cccttcaccg cttccatcga aacttctcaa aagcatcagg
     6361 gtccgtttga tatcatcacc tttgccggca acaacaacag cgacggtgcc agcatgacct
     6421 tggttctgga gacttctttg gacggcagcg actggaccac catcgatacc ttgaagcatt
     6481 cttattacag acgtttctgg aaacgcaacg tatcgcatta cgaaggttcc gatcaagtat
     6541 ttgttcgtgt tacacaagtg tccggaagca ccaaagcggt tgtttacgac attctgttga
     6601 tgaacaatgg cgaagtgtct cagggttacg tggcattgcc cgttcaccgg caagccgccg
     6661 aagtgattgc caccgaatat tataacttga atggtatacg catggcaggc cccgcttatc
     6721 agggtatcac cattgtcaag aagatatatt ccgatggtca tatcaaagtg gaaaaaatct
     6781 ttattaaata attaaacaat caaccattaa ttaaacctat tgaaaatgaa aaaaatcctt
     6841 gtgtttttga tggcagctac gctatgcttc ggcacttcgt acgcagccaa aaagagagta
     6901 gctttcattt accaggaagg ctacaacagt ggtaaggccg ttgattacgg tttgaaagaa
     6961 ggaggctata ctcccgacca ggacccaatt cacaatgccc ttgcggccga gttcgatctg
     7021 actttgtaca acagtccctc gaccgtcgaa gtcgattacg atgagctggc cacctacgac
     7081 tgcgtggtgt tctcggaagc catgccggga aactctaccc tgacgaacgg cttggtgaaa
     7141 ctggtcggca gagtgcccgt cgtttcgatg aaaacttaca actacaccaa cggacgctgg
     7201 tcgtgggcgg caccgagcaa tccgggcacc aggtgtaata ccatcaacat taatgaaggt
     7261 tttgattctc acgccatttt tgccggcctg accagagagt cggacggcag cattgctctg
     7321 tacgatcccg ccaagccggc ttcaagcaac cgtttgcaag gttttgccac ttcgggcatc
     7381 attgcgggtt ctcccatcga tgcggaaaga gataaattgc tggctgttac tacttccgac
     7441 aattacaaca acgtgcatga aataacccta ttgggcaagc cttatgtttt ggtaggtctg
     7501 tccagcgaca atatctgggc catcaacgac aacggtgtca gaataatcat aaatgcagtg
     7561 aagtatgtta tgggtgaagg cgatatcgta atggaagaac acgagatcgc ttacatctac
     7621 gatgaatcgg caacgggcta ttgcggtata gacaatgatc ccgtttacaa cggtaccaaa
     7681 atagccgaga tcaacaccac tgccatcaat atcaaagatt tcaccgcagc cgacaccgac
     7741 actttggctg ccttggaaaa ctttgacgct gtggttttgt gtggcgcttt gaaccccgag
     7801 catgcctttg ttgagatgct gggcaccgaa gtgaatcgcg ttcccatgct taacttcaat
     7861 gctttcttgt ctcaggcctg gggctttggc acgggcgtga atcccactgc taatgcttct
     7921 gaacaaggcg gtattgccca gatcaatgtt gactctgcct accttgacga tgctctcttt
     7981 accgatatga ttatcgagga aggcggtgcc gtggttttat accataatta cgatgcatcg
     8041 ttgatcaaga ccaatttggt acaaggcttc accgccaccg acgagcaatt gttcagcaaa
     8101 ggcgaagtgt tggcaacggt agtaagcgga gaagatactt acaatgccct ttaccgtttg
     8161 ggtaataaga atacgtacat cttgttgccc atgtctttcg aatcgatttt tatagaaggc
     8221 gaaagcaact tgtcggacga tgccgttcaa ttgatcaata atgcgataga tgctattatc
     8281 agcagcaagg gctctgtgac ttcctgtatt atacctagtt gcactttcga atacagcaat
     8341 ctttccactc aggttacctt gagcagtgtt accgaagatg ccgtcattta ttataccttg
     8401 gacggtaccg aacccactgc cgaaagtttg cgttataaag aacctttcac cgtgacggta
     8461 gattctactg ttatcaaggc cattgccacg aagcaaggtt tcaacaacag cggtgtttat
     8521 tcgggtatta tcctggtgaa agcggtggct gaggctcctg tcattaccct ggaagcacaa
     8581 gaaggcaaaa cagtagtaag cattacctgt cccaccgaag acgccaccat ttattacaat
     8641 tacaccggca gtaatattat agcagcgagc gccgcataca ctgcgccggt agaagtgcgc
     8701 tatcccttga ccataaccgc ttttgcaggt ggtggtagct atattagttc cggcattgtt
     8761 tccaaggaaa tcggtgtgac aggtattacc aaagacaata tccgtttaga cactctggct
     8821 tacttcgatg ccaacaatac cgactggttc tgggaaacct cgggtggctc gtccaaagta
     8881 gcctattatg taggtaaaaa ttcgagatct tcttatgcct ccatcgacac cattgtagaa
     8941 ggtaacgata ccacctacaa ctaccacttt atcgatgata tctttatcta tgctcagaac
     9001 gacagtttga atgccaaaga caacggttgg ttcatgagaa cgaaaggtca ggtcattcag
     9061 tgggaaaata cttcgcctct gatggaggta ggttatgccg gtgccaccgg ttattatgcc
     9121 gacgcagtcg aagacctgat tactactccc accaggaacc atatcacctt tggtcctaag
     9181 gtttcgggtg agcctttcac tgcttctatc gaaacttctc agaagcatca gggtccgttt
     9241 gatatcatca cctttgccgg caacaacaac agcgacggtg ctaatctggt actggttctg
     9301 gagacttctc tggacggcgt ggaatggacc accatcgata ccttgggtat ttcttattac
     9361 agacgtttct ggaagcgcaa tgtatcgcat tacgaaggtt ccgatgaggt atttgttcgc
     9421 attacacaag tggccggcgg taccaaagca gtcgtttacg acattctgtt gatgaacaac
     9481 ggcgaagtgt cccagggtta tgtggcattg cccgttcacc ggcaagccgc tgaagtggtt
     9541 gccaccgaat attataactt gaatggtata cgcatggcag gccccgctta tcagggtatc
     9601 accattgtca agaagatata ttccgatggc tacatcaaag tggaaaaaat ctttattaaa
     9661 taaagtttga tctccaaaag ggtagggccg tcaaccagac gacggctctt ccctttttct
     9721 cttctttttt atcttatcga cttattctta atcgatcatg aaacgattag cttctttttt
     9781 cgtttttacc atttgggcaa tgacggtgac ggctgccgat gcgctcccgg cctttcccgg
     9841 ggccgaggga tttggaaaat acgccgtggg cggacgttac ggatcagtct atcgtgtgac
     9901 caacctaaac gacagcggca cgggttcttt acgcgatgcg gtcagcacgg ctaatcgtat
     9961 cattattttc gacgtttcgg gtgtgattaa gctcaacagt cggttggttt tcaaaaacaa
    10021 cctctatgta gccggacaaa ctgctccggg tgagggtatt accgtttacg gcaatggcgt
    10081 ttcgttttcg ggagcgagca ataccattgt ccgttatttg cgattccgta tgggctccgg
    10141 cggcgatgcc ggcaaagacg cagcgggcgt ggctaacgga acaaacatga tattcgacca
    10201 cttgtcggtg tcgtggggat tggacgaaac tttctccatt tcttcagacg gcaagggcgc
    10261 tctgggtgat attaccatcc aaaacagcat catgagtcag ggattgatga ctcactcggc
    10321 cggaggattg atgcaggccg atcacattac tttgtatcga aacttatacg tggacaacag
    10381 tacacgaaac aataaaatca agggtactaa ccaatatgtc aacaatattg tttacaattg
    10441 gaagaatggt tgctatatta tgggaggcga ctctcagggt accagttatt gcaatgcaac
    10501 caacaactta tttatcaatg gcccggcggt aggaggcaac gcttttacgg gcggcaacag
    10561 caatttcaat atctacgccg acgacaattg gcaagacaaa aaccgcgacg gcatcttaaa
    10621 tccatacagc attcctcagt cggaatattc cgggccacct acttttcagt cccaacctta
    10681 cgattacccc gccttacctg cttatccggg caatgctttg atagacagtc tgctcccgac
    10741 ggtgggtgct tcgttgcctt atcgcgatcc ggtggatttt tatgtgataa acgaaaccat
    10801 gtcgtttggt aaagaaggag ctttgatttc caaagaagcg gaactgccca taggtattcc
    10861 cacctcctgg ccgcatcagt cttttgccaa accggtcgac acagacggcg acggcatgcc
    10921 cgatgcatgg gaaacagcca acggtaccga ccccaccgtc aacgatgcca tgaccattgc
    10981 agccaatggc tatgccaaca ttgaaaacta tatcaacagc attactatag acgaccgcgc
    11041 cttcttcttg cgccgtccct ttgtattgtc cttgcaagaa tcttctcaga atcaattggt
    11101 catcaattgg ttcgactaca ccgaaggcga agacggcttt atcgtcgagc aactgatcga
    11161 cggcaattac accgaagtgg cacgcatttt ggccgccgac tataaactca ggggtaccga
    11221 cggaagtgct ccgggcagtg ccgccggaga gagaacgggt tcggtcgctt gcaccgtcaa
    11281 gaacctgatg cctgccacag cttattcttt ccgcgtcaaa gcattcaaaa cagagggtaa
    11341 ccatttctcg gaatattcgt cggttttgga agccaaaacc caacccgaat acgtacctat
    11401 ggtggaatgg gatagctacg aacccgattt gatttggaat atcgcctcgg gcgcatggaa
    11461 ttttactgac ttgaattggg gtgaaaatgt ctataccgac agcaccgctg ttttgctgac
    11521 tccttccgag agtgccacta ttaccataga ctcggtggtg tcgcccaaaa ccgttgtggt
    11581 agggcaagac cacgacctga ccttggcggg ggcagccatt gcgggcgaag ggacttcggt
    11641 caataagttc ggaaaaggca agctcgacat gggttcggcg gctcacactt atacgggtgc
    11701 gacggccctt cacagcggcg tgttgcgttt cgctacgctc aaaaacggtg gtgagaacag
    11761 tagtatagga gcctcacagg aatttgcaca gaactggatt tgggatggag gttgctggga
    11821 atacaccggt ggtaacacat cgaccaaccg ttcggccaaa gtctatcaaa acactacctt
    11881 taatatcagc agtggcgcca cagtaacgat gaccggtact ttggaaggta cgggcggtgt
    11941 caccgtcaaa ggcggacaaa tcagcgtggg cagcacttcg ttttttggtt acgaaggtcc
    12001 cactcgtttg gaagaaggcg gtagtattta tttgagcacc accgagatca gtaaagtcgg
    12061 acttgggaaa tcgtctcaat tgattttggc gggtggaaaa ctcagcacca aaggcgagag
    12121 caacaactac gaaacctatt ccttccccat tcacgccgaa tcgggcacct attcttattt
    12181 tgcacccaat cgcaactgtt ccattaacaa tgtcgtgacg ggcgacggta ccatcgaata
    12241 ccagattcca tatgttcgcg aatacatctg taaaagctgg gacggcttta cgggtaaact
    12301 catcgccagg ggtatgagtt ccagctcgga tggttcgcaa ctgatgctgt acaacggcat
    12361 tggtattccc aacgcagtgg tcgaattacg tggcaatacc cgcgtgattt gctggaaagc
    12421 ggcagccact tactatttgg gcggattgtc gggtgcatcg ggtacttatt tgtcgggagc
    12481 gagcaagaac accagaaaca ctaccatgac ctggattgtg ggcggtgcca ataccgacga
    12541 aactttctgg ggcgtcatcg acaaccgctg cagtgctgct aattatgatt gtaccactca
    12601 cattcaaaaa gtgggaactg gcgactggcg actggcaggt acgagtatct attcgggcag
    12661 caccacggtc aacggaggtc gtttggtggt aaatggttca ctgacaggca acggagccat
    12721 cgcggtgaac aatggtggta cgctttgcgg caagggttcg gtcaaaggca gagtcactgt
    12781 gaacaatggc ggcaaagtcg ctgtgggaga cacgcttttt aataagtcgg atgtctttac
    12841 actgaatggt ggtttgaact tactcaacgg aggtataatc caggtgccct tgtatcgtaa
    12901 acagatattg aataacgctg ccagaattaa atttgccgct aacagtacat taaacggtac
    12961 gctcgaattg gacatgaccg atgtgacgat agacattccc aacaatagtt cgtttactat
    13021 ctttacgatg ggaacaggtg tgactttgag cggaagtatc tctgccgttg aacctgccga
    13081 accttcggaa acgcaggaat gggatttgtc cgaattgttt accacgggta agatttatgt
    13141 tcgcgagaag ggttacacgg ccttgcctca ggtaagcgtg gttcccgtaa gcgaacaata
    13201 tttcagtctg gaaggcgtgc ccctgggcat ggagcctccc tacacggggt cgcctgctct
    13261 gtttattgtc aaaaagaaat atcaggacgg cagtgtccgc atcgaaaaag ttatggtata
    13321 ttaaagcaac aacataaact atttaaacaa tgagaaaaag aatcttcttt tttagtttga
    13381 cggggatctg tctactgctg acagtgttga cttcttgcca ggaggaactg gccaaccgtc
    13441 cgcattatgc tccccccgac tggttaaagg gaagcgccta cgaggtgctt caagacgagg
    13501 gcaactacac gatgtttttg cgcggtgtag atttggcggg ttacgctccc attgtaaaag
    13561 gcaagagcat cctgacggtg atggccccca acgattctgc cttcacggcc tatctgcaaa
    13621 aagcaggtta tgccggcatc gaccaaatgc atgcagagaa cccggaacag ctttcaaagt
    13681 tgataggatt tcatttgctt tattatgcat tcaactggga taatttagtt aatttccgtc
    13741 cgatagaagg cgacgaagcc tccgccgaag ctttgttggt gaatgcgggc tgctattaca
    13801 agtttcgcac caaaagcgcc gaccccattt ctatggaatg ggacggaaaa gactctgtac
    13861 gcgtatatca ttttgaacgg tttattcctg ttttcaacac acagttgttt gctaccagag
    13921 gcatcgaccc caaatacaac tatgagtact ttttccccaa taccgagtgg acgggcagcc
    13981 atcacaccgg aaagatcgat ggtttcaatg tgtcgaacgc gtcagtgata gacgacgatc
    14041 atgttattac cgataacggt tatctttatc atatcagtca ggtgttggat ccgctcgaaa
    14101 ccatttacac cgagttgaag aacaacgccg attattccat ctttttcgag ttgttcaatt
    14161 cttacggtga gtatcaggaa gaacccgaac tgactaccga ttttggcaat ggacaaacct
    14221 tgtatctgca cactcatccc ggtttagtga acattgcctg cgagtggccg gtgaccgatt
    14281 atactgccat ggacaggttg tcgagtgtgg gatataattt gtttgcgccc tccaacgatg
    14341 ctgttcagaa ctttttcact acttattgga cgcccggttg cggttacagt aatctgaacg
    14401 aattagaccc gcttatcttg caatatttta taagccagac ctttggaagt gacgacgtgg
    14461 tagcctttcc cgaagaaatc aagaaaggtt tgatcaagac cgtttacggt acgcccgtca
    14521 acatcgatcc cgaccaggta acctggaggc aaatgtgctg caacggtgct ctgtacggaa
    14581 tggaccggat gacgcctccc gccattttct cttcggtgat aggcagtgct tttcgtgata
    14641 aacgattctt accttattta tatgctttag acggctcgga tttggtgttg gcattggctt
    14701 ctcaaaacac cgaatttgtg gctttgattc ccgatacggc tcagttccgc ggtgccgcca
    14761 tacgtttcga aacgatagcg ggcggtcgtc atgaattgca agtgattggc gacgacggca
    14821 attatgccgc catgagcgtt ggaaccatgc gcgatatggt taatatgaac gtgtcatctt
    14881 cggtaaacca actatcgact accggttttc aggtggtaga aaccaatacg gcattcaact
    14941 attggtatct gtacgatggc aaaattacca ccagtgcttt gtttaataaa cagttgaatc
    15001 ccacctacgt cgacgaaatt tacttcgatt ttgctcccct cgaaatgaac ccagctcagc
    15061 ccaacggggg caaatgggac aacggaagcg tttacaccta cactgctccg agtattttta
    15121 tgccgggcga ggacgaaggc ctgaaccgca cccttgcggt ttgcaacgac aacacttatc
    15181 cttattattt gttctcacaa ttgctcagca aggctggatt ggtcgaggaa caacaattaa
    15241 aaatagtgat ggatgatgct cgtttcatag cttttgtgcc taccaacgaa gccatcaaag
    15301 cgcgtatcac cgagatcccc ggttgcgaca agtgcagcgt ttccgacgat atggtactga
    15361 tcggtaaccc caacaaagcg ctgctggcta attatcttcg cagttatttt attatcagcg
    15421 atttgactcc tttcagcgtt tatccttatc cgggcagcgg catgaaaggc agtttcgata
    15481 catcgggagc ttacaaactc aatatcaccg atacgggtac gacgctcacc gttagtctga
    15541 ccgacaaagg tggcataaaa ggagcggaag tgtctttgta caataccgaa aaattccatt
    15601 ccttgccttt ctcgtacagc gacggtgctt tccatttaat caacgacatc ttgttataaa
    15661 ttggccatta tgaaacaaat aaagatattt ttgatagtgt tggtcgctgt attggtctgg
    15721 ccgcagttac aagctcaaaa tacggtggtg acgggtacgg tcactgaatt gtacgacggc
    15781 aaagtagagc ctgtctataa tgcgaatgtc gtggtggtca gttccaataa ccgttatata
    15841 acaggtgcca ttaccaatat cgacggacaa tacagcatcg ttgttcctgc ttacgaaaag
    15901 gaaccgcgca ttcgattctc ctacatcggc ctgaaaaccc aaacgatcga ctatacggga
    15961 caaaaggttt taaatgtgcg tatggaaccc gacagcaaaa gtctggacga agtacaagtg
    16021 acgggcagga gagtagacag agatgaaatg ggtatttcgt tcacggagca gacttcttcg
    16081 acgcaaaaaa ttgaaatgtc ggatattgtg cagagtacgc ctatctccga tatcaccgaa
    16141 gccctgcaag gccagttggc gggtgtggat atcgtggtgg gcggcgaccc gggagcgcgc
    16201 agtgccatcc gtattcgtgg tgtcaattca ttgaacgctt cgtcggagcc gctcattgtg
    16261 gtcaacggta ttccttatca cgtcgatatc gccgacgatt tcaattttgc cacggccaac
    16321 gaagaagact ttggcgcctt gctcaacatt gcaccctacg acatcgaatc gatcgaggta
    16381 ttgaaagatg cttctgctac agctatttat ggtactaagg gtgccaatgg tgtcttgttg
    16441 attaccacca aaagaggttc aaaaggcaag accacttttg gtttcaacag caagtttacc
    16501 accaagtttg aacccgatcc tattcctttg ctcgatggta atcagtatgt ggctttgatg
    16561 caggatgcca tttggaatgc cgccaatgcc aagggtttga catcggccag aaacgaattg
    16621 gaacaattgt ttaataccta cgatatcaat tatgtcagag actggcagta ttttgatgaa
    16681 tacaatgtga ataccaaatg gttggactat gtgcgtcaga atgcttacac caccgacaat
    16741 aacttttcgc tgtcgggagg tggcgacaag gctacttacc gttattccct gggttatctg
    16801 aacgaaacgg ggaccacggt gggaaccgca ttgaaccgtt tcaacacctc gttcaatata
    16861 cgttacgact ttagcgagcg tctcatggta aacaccgagt ttacgtatac acagaccaac
    16921 agggatgcta atgcactttc gacgcttcgt tccgaggcac aacgcaaaat gcccaacaaa
    16981 agtccctatt acatcgacga tttcacgggc gaagccttgc cgagctattt cagtcgtcag
    17041 aacgaagatt tccagggcgc cttcaacggc agttatcatt ataaccccgt ggcaatggcg
    17101 gagatgggtt tcaatagaac catgcagcgc gaagaaaaaa tgactttgaa cgtgcaatat
    17161 gttttcccct tcagtctgat gtacaaaggt tatgtgtcga tgaatatgcg tacttccaag
    17221 aaccgcaaat tcattccgca agaagctacg ggcgtactct ggaccagcaa ctatgccaac
    17281 cagagtaccg atgctttctc agatagaatg accttgcaaa cagagcacaa attattgtac
    17341 aacaagactt ttagagatgt tcatgcggtg gtggctaccg ttttggccaa tatgtcgcaa
    17401 aacgacagtt acagttacac cagtgctacc agcggcaacg cctcgcccaa tttgagcgac
    17461 ccggtagcgg gaagtgtggt aagcagtatc ggctcgggtt tttcgcaagt acgcagtgtg
    17521 cgtgcagtgg ctcaaggggt ctatacctac gatcgccggt atgttttcag gactaccgtc
    17581 aaccgcgaag gtaactctgc catgggtaag agcgaacgct ttggtacttt ccctgcattc
    17641 ggtgtggcat ggaatatcca cgaagaacct tttatacgcg acagggacaa tccctggctc
    17701 accgaaggta aattgcgtgt cggtttaggt tggagtggta atgccccttc gggtgcatcg
    17761 atgtacctgg gtgcatatca gtctttaggg gaatatatga gcatgccggc catttatcct
    17821 gtccgcatcc agctcgataa actcaaatgg gaaacctcgc gcgagtggaa ctatggtttc
    17881 gacctggaat tctttagaaa attgggcttt acgttcgacc attacaataa atacaccagc
    17941 gatttgttgc ttggaaacgt cagcattcct tcgtcaacca gttacaccag cattaggtac
    18001 tttaatagcg gtgaactgag caacaagggc tacgaattcc gtataactta caatgccatc
    18061 aacacgaccg actggaaatt gagcttcaac tttaacatca gtcacaacga gaacaaggta
    18121 gaaaaactgc ccgccaatat gaaggaagaa aactacacat ttggcaacgg taattatgcc
    18181 gtgcgtgtga tagagggcga ccccatcggt tcgttctatg gctaccgtta tttaggtgtg
    18241 tacgacaata aagaagaaac ttatgcactc gacgaagaag gccgagtgat gcacgactac
    18301 aaaggtcggc ccattgtaat gaagaatggc tccaccaccg tgtttcccgg cgatgcacgc
    18361 tatcaggata tcaattacga tggtgtaatc aacgaatacg atatcgtata tttgggnnnn
    18421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacga gtttgataag agataacgcc
    18541 tccacgcctg tgaccaagcg tttggcattt ggttttaatt tgaattttta aggagggtac
    18601 acacaatgaa aacaatgaat agatttcttt tgacgacgat gaccttgttg gccctgacac
    18661 tcagttcttg caacgagtgg ctgaatattc tgcccaacaa cgaacaagtc accgataatt
    18721 actggaaaac ccgccaggat gtagaagcgg tggtagcctc cggttattac tatatgcgtc
    18781 aggcagtacc caccatgctg gtatggggcg aggtgcgcgg cggtgctctg tattcgagca
    18841 acagccaaca cagctatctg caaaacttca acctgacacc ttcgtcgggc atttgcgatt
    18901 acgcctcctt gtataaggtg ataggtatgg ccaacagcgt gttgaaatac gcgccctcgg
    18961 tgacaaccga agacgaaacc tattacgaaa gtgtgatgaa atcgcatttg tgcgaagcat
    19021 acttccaacg tgcctattgc tatttggttt tggttaagaa ctttcgcgag gtgcccttgg
    19081 tggtcgaggc ttttgtcaac gacgatgccg attacatgac acccaaatcg agcgaagccg
    19141 atatcattgc gcaaatcaag gacgatatcg aaaaggcgct tgcaacgggt gcagccaagg
    19201 atatatacga agacaccgac ggttacacct ggcaaaccaa aggccgtgct accaaatggg
    19261 ccctgtatgc cctgatggcc gatgcctgtt tgtggaacca cgactacgac gaagccatcc
    19321 aatattgcaa ttacattctg gatgcacccg aaggcgacac ttcgttccgt cctcgcttta
    19381 tgtcggatat gaccaaatgg tacgaaattt tctatccggg caattccaac gaaagtattt
    19441 ttgagttgaa ttgggaaact catatgtctc aaaccaacaa ctttggttcg tggttctcgg
    19501 taggagcctc atccactttg aaggttaccg atcatctcaa ggctaaattg attgccgaaa
    19561 ccgacgaagt catggccaat tcgattagaa acggctgggc taccgacggt cgggtggggc
    19621 gtatgatttt tggcaccttt gtgtccgatg cggctgacca gaccaaatac ggaacagcca
    19681 attacctgtc ggtatggaaa tacatgggta ccgatattgc cgacagaggc aacacgcgtc
    19741 ccgctcagga tgccaacttt atactgtatc gtgtgcccga tgttatgctg accaaggcag
    19801 aagctttggt aatgaaggga ggtgcgcagt cctggcaaac agcattggat ttggttaacc
    19861 ggattcgcca gcgcgcaggt ttggattcgg ttaatatcaa tgtcagcgaa accgacgaaa
    19921 tggtgatgct cgacgctgtt atgaacgaat ggaccatgga gttcctggcc gagggtaagc
    19981 attggtacaa tctgctccgg atgggacgct acgacaaggg cgacggtgtt tacaaagaac
    20041 gcttcatcaa cgaaatcatc gagagcaacc agaccaccaa agacgagtgg atcaaatcgg
    20101 tgttgatgga cgaaaacgcc tggtacatgc ctattccgta caacgaaatt tcggtgaacg
    20161 aaaatttggt tcaaaatccg tattataaaa ccactaaatg acaagcctta tgaaacagac
    20221 aagcataaaa atattcatgt ctctggcggt cgcgttgttg ctggcggctt gtcaagacga
    20281 caaactgaac agtgtttatc agatattcga cgataatccc gcttccaaaa ctttggaaaa
    20341 aaacgaaaac cactcggaat gggtaaagat tttgcgttat tccgatatgt acaatgcctt
    20401 gaaccaggcc actcaagctt ttacgctgct ggttcccgac aatgatgccg tttttgcctt
    20461 ttatcagcaa cgcggcgtgt cgggcattga agatttgggc aaggattacg ccaaagcatt
    20521 gattctgcat cataccatgt tggattccat catggtggag tcgatgcaat taaagaactc
    20581 tttgaacaac ctgaacggcg atgccatcag cgtggccatc gacactctga atccggggca
    20641 gtttatcttt ggcggactgg cccgtaccac agaatcgggc gtgcatgcct acaatgcgct
    20701 gatgtattac attgatgcag tgcttacccc tctggtggag agtgtttacg acaaactggt
    20761 cgaagacggc cgctatggta tcatgaaagc agctctcgac gcaaccgcct ggacgcaaca
    20821 actccatacc atctacgaca ccgtgcctca agagggtgga ggttatgtgg taaacagacg
    20881 tgcttacgca ttgctggccg tatcggacga aggcttcagg agaaacaata ttcaatcttt
    20941 caacgatttg gtagccaaat tgtccgcggg aaccgactac actgaaccgg ataatgcact
    21001 gaacgaatac gttgcctacc acattctgac caacaattac actttggcag acctcaagga
    21061 aatgtcgggc gacacttcgc gcatttggga aacttctgct aaaaaccagg tctttatgat
    21121 aacggtagat accacagcgg gcgattggca attcaacatc ctggacagcg ctattttacc
    21181 cacccgtttt gtggaagaat attgcgacgt cctgtgtaag aacggttatc tgcacgaaat
    21241 agatgcctat ttgcctgttt gggaacccaa accttcgatt gtggtttggg atttggccaa
    21301 ctacatcgag gtaaaaaaca cggtgattga atctgcgggc cctgaagaat atcaaccggc
    21361 cgaacctgtg tcgtccgaaa agaaaataga catcaccaaa tgcggcgcct acgattatgt
    21421 ggtgtcggaa tcgggtgtag gaggcagcag ttacgcttac attacctatg ttacttgcaa
    21481 aaacaatctg agagattgtc tcaaatacga ccgcgtggtc ttcaatctgg gttatatggg
    21541 ttccgtttcg atgaaaaccc cgactttggt gcgtggaaaa tataaagtgg aactgaattt
    21601 cgtttatttg agcgaccaca actttatgcg ccagatgaca gacggcaacg gcggtatgat
    21661 aaggatgacc atagatggcg aaaatctcaa gagtgtgacg ccatacacca ccgttccaag
    21721 aaatgttgca aaagtgtata cagctacttt gtacgacgaa gtagagtttg agcagacttc
    21781 cacccacgac ttcaaatttg tggtgatgga tcccgctgcc agcaccaaca gcagattcag
    21841 tctgcaattc gattgtatca ccttcactcc catagaataa aggcttatga aaacgataaa
    21901 ccgcatttgt ttaaccggct gcatgcttct ttgtctgact gcttgcgacg aaaattggca
    21961 acggcattat tatcagtcgg agacgaccat aaccaacgag cagcttatca tggttagtca
    22021 aaccactcag gaatacctgt cgggcaacga tagcaggcat ttgagcgata tgtatcagtt
    22081 tttacaagac aacaaggtgt tcaacatgtt gcagaaaaaa ggacagttgc acactttgct
    22141 gatggtagaa aacgatgcat tcaaagctcc tgaagccgag agagcagaat atattgccca
    22201 ttcgcacgtc accgatattt gtttgtcgcc aagcaacctt tatcacggtg agcgtgtgct
    22261 aatgtggcat cagaaatttg ttaccgtcgg tatcgactcg cttggattgt tgggcgattt
    22321 gacgcatatt acttttaacg gttcgcccgt aaaagaagta gtcaaaacca ccgatggtta
    22381 catttatgtg ttggacgcga tgatagaaac acccatctct ctgcaggatt atatcaacga
    22441 tttgcccgaa gaatacagtc tgttcaaaga catggtgctg gcttccggcg gcaagctgtt
    22501 cgataagaat aacagtaagc ctatcggtat cgatgccacc ggnnnnnnnn nnnnnnnnnn
    22561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22621 nnnnnnnnnn nnnnnnnnnn nncaaagcct cgatctggac aatcccatca aagtgtccaa
    22681 cggcattgtt tacgaagtca agaaattacg ctttccaaac aatatcctga tttatcgtat
    22741 caaagatttt tatcgcaatt atgaatattg cactgccgag gagaaggaac tttatttcaa
    22801 gtacgacaat ctcaagaaca tcgaaatgaa atcggaagtg gaagcatgga cgcccatgcc
    22861 gggtgtgtgg cccatgcacg gcaatgtcac tttcagttgc tatctgaccg atgcttcgca
    22921 aggcttctat ctggatatgc agttgattaa acccgtctac gacgaagcgg gtaatatcag
    22981 cagtgtcaga ccttatttaa tcccgccggg cagctatcgt tttgccatgg gttttttcca
    23041 gaatatgggt gtcactatga atgtgagcgt gattgttttg aacggcagcg aacgcgtcaa
    23101 gttgggcgag gcttctgtga cgcttgcctc caccaccgag tttcactacg accgcggcac
    23161 tttcttgtcg gatacttggc ccgaaggcta taaagaggtc aagaacgatt atactcatag
    23221 caaaaagaac aattacaaca ccgacggcgg agcagttatg gccgagctgg aaatacccga
    23281 cctgaaagga gacggatcgg cacttcccat gatcatcaag ttggaaatgt ccaccggcaa
    23341 agactcccgc ctggcgtttc accattggtg tttgcgtcct acaagtgata actattaata
    23401 ttgcagataa tgaaacagtt ttatacaatc ataacggcac taatgctggc cttgcccacc
    23461 ttggcgcaag ctgtggaagg aatcgttatt tcgtccgatg gacaagcagt acgaggcgcc
    23521 gtgctatcgt cgccgggaca aaccacagtg gtgacaggag aagacggaac gttccgaatg
    23581 gatttgcaaa aagacgctgt gctgtcggtg tgggcagatg gttattacta ccaacaaatt
    23641 cgttacaagg gacaatcctc actaaaagtg gttctggcac gccaggatgc gcataaatac
    23701 accgagtcca aagtactgcc ttttcgtgtg gaacagaatg tcaatttgcc ggaagtcaat
    23761 actttgtcca agaaagattt tgcattgggt gctcgttcgg tggaacaagc tttgcaaggc
    23821 gaactcgccg gtctgcaact gatcaacaag agcggcatga cgggcgaagg tgcttttttg
    23881 tcgcatcacg gcattcagac tctggcagcc aacgcctcac ctttggtggt aatcaatggt
    23941 gtgccgttta tgccttctat ggatgaatcg caattgatag gcggcttttc taaaagtatt
    24001 ttccaggctt acaatatcta cgacattcag gatatctcgt tcctgagtgg ggcagaggct
    24061 tcggtttacg gttcgctggg tgccaatggc gtgttgctca tcgaaaccga cggagccagt
    24121 tccgacgata tggataccaa gatctcgtat catggaaagt ttggctataa ttggaacgat
    24181 cgtcgcttgc ctatgatgaa atcatcgcag tacaaatctt atctgtcgga catcggtatg
    24241 acctattaca acaatatgga aactttcttt gcgaatttcc cgtttttgtc gcccgacgag
    24301 agagatgctt acaattatct gtatcgcttc gacaccgatt ggcaaaacga attgttcaga
    24361 cgtggcgtaa ccaccgacca tttggttcgc gtagaaggtg gtgatgctat tgccaaatac
    24421 gatatttcgc tgggctatca acacgacgaa ggtgcgctga aaaacacaca gagcgaccgt
    24481 ttcaatgcgc aaatcaattc gaacgtcatt gtcagcaaaa agttcgaaat aggcacctct
    24541 atcggcctgg cctttttgaa cggttcgtat caggaacaag gcctgatcat ggaaaccaat
    24601 cctttgctgg ctgcttatcg tcgagcaccg gtgcttagtc cctacaaaaa cgatatggcc
    24661 ggcaacctgc tcgaaaccta cgcatcgtat tattacggca acaacaccaa taccgatttt
    24721 attgtcagca acccccttgc cattgttaac acgctggctg cagacaagcg tcagtacgat
    24781 atcaatatca acgcccgagt tttgtataaa ccgaccaatc agttgtcgtt catggccttg
    24841 ttggggcttt attacaacta cgaccaggaa ggtatctttg tgccgggtat caacaacaag
    24901 gatgtggtgc ctcagttcga tacctacggt gagagtaaaa acatagtgcg tcagggagtc
    24961 aacgaaacct ttaacagttt ctatcacctg aatgctgcct acaataatgt ttttgacgat
    25021 gttcatggcg ttgacctccg tttgggagga caaatcatta gcaacagttt cgaaacggat
    25081 atgggcgtgg gccgcaacac cgccaacgac ttttatcaaa ccctggccga tgtgcaaagg
    25141 gtaggccggt tctttagcgg ttacggagct aaatggagct ggctcaatgc ttatttgcaa
    25201 gccgcataca cttacagcga tttgttccgt gtagctgcaa acgtctccac cgacggtgcc
    25261 tcttcgacgg gtgtcaatac gacaagaatg ggtgttttcc cctcggcttc cgccacagtg
    25321 atgctggcta atttcgactt tattaaatcg gcagaggccg tcaaccgcct gaatctgtac
    25381 gcagactacg gcaagacggg caacagccgt ttctcccata cttacggtag ctattattac
    25441 gtcagccgtt tgtatcaggg cattgcgggt attgtgcgta acaatgtgcc caacaccgaa
    25501 atcaaatggg aagaaaacct caaagctaac atgggtatcg atatggccct gtggcgtcat
    25561 cgtgtacaaa taaaattagg ctattacaac aatcgcgcca ccgatgtgtt gatggcgggt
    25621 tcggaatcgg cactttacgg tacaggtgtt cattatgtca acgatgcagc catcgatgcc
    25681 aatggtttcg atttgtcgtt gaatgtgtcg cccgtttgca ccggaaattt caaatggatt
    25741 ctgggtggca atatcacgca gttgaacaac aaggtgacct cgctgggtaa cagaacccat
    25801 ataatcaaca aattgtccga tggagcagcc atcatcacca aagtaggaga gagtccctat
    25861 agcttctatg gttaccggac cgatggcgat tatcccgttt attctacggt aaaagaagcg
    25921 caatcggcct acaaatacac ggaaaacgga gtggaataca gccgacccat gcgcaaccgc
    25981 aacggggtgg aattccaagc gggcgacatt aagtacgtcg atcagaacaa cgatggtatc
    26041 atcaacgacc tcgacagagt gctgctcggt tcggccacac ccgatttcta cggcggcttg
    26101 ttcaaccgtt ttcaatacaa aggctttgcg atcgatttga attttgttta ctcgttgggc
    26161 aacgaagcct acaacgcggt gcgtcgcatt accgagtctt cgctggacat gagcaaccag
    26221 agtttggccg tgttgcgccg ttggactgtg gacggacaaa ttaccgacat gccgcgtgcc
    26281 aattattacg atttggtggg caacaacgat tttagcgacc gctggatcga agacgcttct
    26341 tacctgaagc tgcgcgatat tacctttagt tataccttcg acaatatttt gctcggattt
    26401 atacaaggcg gcactcttta cgtcagtggg caaaatctgt ggtgcctgac accttatttg
    26461 ggcaacgatc ccgagttctc ctattcgtac agcgccatga tgcagggcgt ggactatgcc
    26521 aagctggcag ctcccaaaac cgtaacgttc ggagtcaatc ttaagttcta agcattaaac
    26581 catcaagatt atgaaacaga acatgatata tcgtattttt gccgcttcgt tgttagtgct
    26641 ttcgatgacg gcttgttccg gttttttcga atccaaaaca gacgatgtgc tttctgcgga
    26701 cgattatatt tcgtcgcaaa ccgaggtcta tacgggctat ctgggcatca tcaccaaatt
    26761 gcaggcggtg ggcgacaagg ccatctacct gaccgataca cgtggtgagc tgcttgagcc
    26821 taccgaaaat tcattgcccg aactcattgc tttatataac tacgaacagg atttgacggg
    26881 taacggatat gccagtcccg atgcttatta cgaggtggtg attgcctgca acgattattt
    26941 ggagaatatg cgcgctttcc gcaaggctcg tccggaactg gtcaacgatg agcgttttga
    27001 tgccttgcag agtcttactt tgcgtgtcaa ggtgtgggct tatcttactt tggcagagat
    27061 ttacgggcag gccgtctggt tcgacgatcc cattgtcaag attcaagatc taaccgatac
    27121 cggcaggttc cagctgatga acatcgatca ggtggtagac cgttgcatcg actttctcga
    27181 taatggcgat gcctctaccg actacatcaa cggaaaattg gacttttcgt ggtatcgctg
    27241 gctcgatccg gaaacggcct tggccagcag tcagtatcgt tattgggatt acatgacccc
    27301 gccattcgag ggcctgatgg ctaagctctg tttgtggaaa ggagccgcat tgcagggaaa
    27361 gggcgattac gacggcgccc ggccttatta tcaacgctgt gccgatttga tattagaagc
    27421 tttgaacgaa gttatttctg tccagagcaa cgccggcaac ggttattatc tgcctactgc
    27481 ttacgttccg ggtcgttatg cacgtttctg ggacaatgta gaaccttacg ctaccgaggt
    27541 gatttgcgcc attatttacg attatacccg taatcaaacc aataaacttt tatatcattt
    27601 cagctcggaa taccctaacc aatatctttt gcgtcccaac gtggccatgg tggaagaacg
    27661 tttcgaaacc gacgataaca atccgggctt ctctaccagt gtcgaggcac gaaacagagt
    27721 ggttttgggc gaccgggaag gcaagcctta cctggcaaaa tatcgtccca ttggcagtgc
    27781 acgtcgtgca aatgcctatc aggacgatgt gcacatctat ttgttccgtt gctttgagta
    27841 ccattttttc ctggtcgagg ctttgaataa tctcggacgc ttcgaagcag cagatgccgt
    27901 ttttaacgga ggtttggcgc ctaccgactt tttgacggca gcagacggca gcatgattaa
    27961 agactccgat ggttacggta cacccaaacc cggtttcgaa ggctttatca acgactggac
    28021 atcggtggct tcctggggaa cccgcaagta cagccatatg ggaattcgcg gctgctttag
    28081 tttgaaagat cgtccctttg ctatagagta ttacaccgaa gcaggacgtc gcgccaatat
    28141 gaaagccaac gatatggcta tactggacga agctatgctg gagttcgccg gcgagggcaa
    28201 ggtttatccc atgctgatcc gtatggccaa acgatggggc aacggcactt acgacgccac
    28261 catcatttcg tcgcgcgtag cccccaaata cggaacaaga gccgccgaaa tgaaacctaa
    28321 aatagaatcg cagttttttg tgccttggga tttgttgctg gagtaggttg ccctagctcc
    28381 tctgaccctc taaaggtcag cggcaatttt tctattatct cacatcggca tc
//
LOCUS       DAOSTD010000018        28010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1877_length_28010_cov_12.998498, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000018 DAOSTD010000000
VERSION     DAOSTD010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 28010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28010
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1877_length_28010_cov_12.998498"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLI37_02130"
     CDS             complement(<1..336)
                     /locus_tag="PLI37_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ57849.1"
                     /translation="MLRNSLFSLVVFCLFGLQAWAKTTTYTDITEADSVLQLAASAYS
                     ESRFDDAAVLYEQVLANYGISAKVYYNLGNAYYKSGSVAPAILNYERALLLNPGDPDV
                     RFNLEMARSR"
     gene            complement(323..2167)
                     /locus_tag="PLI37_02135"
     CDS             complement(323..2167)
                     /locus_tag="PLI37_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOQ57850.1"
                     /translation="MKSRYIGIAFTTILMIFGMELAAADEVVFTATAPSKVILGQQFR
                     LTYTLNAETKDLRIPEITDFEVLMGPSTAKSTKISIVNGQRTSLVEHSYTYVLMPVKV
                     GSFTIPAATATIKKEKRSSNPLTITVLPQDKSTGGASDQGTSSQQTVGFNGEQAFIRA
                     IPSKTKVFEQEGLLLTYKLYTRIDISGVNNIKFPEFKGFLAQEIEIDPNTQWDMENYE
                     GLNYRTAVLKQTILYPQQSGEIEIESGSFDLIFRIRNTNQRMRSLFDDFFDSYQEVRK
                     TILSPKLRIQVEPFPFGKPANFNPFSGNLRLSSSINTTQVKADEAVTIKLELRGEGNM
                     KMLRIPELKFPADFDVYDPKVTNNFKTTTRGVSGTKTIEYLVIPRYSGSFTIPPVNIS
                     YFDLTDKSYKILSTDEFQLEVGRSEQNKEGVVAGTVVSREQLRLLGQDIRYIKVDNIR
                     LRPRTNLIYGKTAFWLAYILPFFFFLLMLYIYRKQAKANADLAAVRTRKANKVAVKRL
                     KKASFYLKNQDKEQFLDEVLRALWGYTSDKLNIPVSQLNKDNIEAQLNRRKVDAEVCA
                     EFLQILQACEFERYAPVTEPQHMGDLYTRTLEVINQMENTINNVHVKK"
     gene            complement(2214..2909)
                     /locus_tag="PLI37_02140"
     CDS             complement(2214..2909)
                     /locus_tag="PLI37_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ57851.1"
                     /translation="MRSIGIFVFLFLFGLAGFAQKAERKHIRQGNSLYEEEKFTEAEI
                     EYRKSLEVDRQSPEGIFNLGNSLFKQEKPKEAIEQYQQITGTEKDSMRLSSAWHNIGN
                     SFMQAQDFGKAIEAYKQALIHNYKDDQTRYNLAMAQAMLKKQQEQPQQEQQQQEEEQK
                     QEQQEQQEQQEQERPEQQNQPDQQEQQQEHISREKAQQLLDALMQDEQEVQEKVKKLQ
                     IQNSRPKKTEKDW"
     gene            complement(2911..>3684)
                     /locus_tag="PLI37_02145"
     CDS             complement(2911..>3684)
                     /locus_tag="PLI37_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOQ57852.1"
                     /translation="KLEKQKRQGAEVIVALDVSNSMMAEDLVPNRLEKSKQTLSRLID
                     QLANDRIGLIVFAGEAYTQLPITADYISAKMFLNTISTEMVPTQGTAIGAAIQLALRS
                     FGLAQEAERAIVVITDGENHEDDAVLQAQEAQKQGVKVHLVGMGSLKGAPIPRGLNDF
                     HKDKEGNVVITKLNEEMCQQIAMAGGGVYVRADNTNTALRVLSKELDSMKKSDIETTV
                     YAEYDEQFQALAWIVLLLLFVDMFILDKKNPALRNLKLF"
     assembly_gap    3685..3784
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3785..4692)
                     /locus_tag="PLI37_02150"
     CDS             complement(<3785..4692)
                     /locus_tag="PLI37_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOQ57853.1"
                     /translation="MIFAQPYYLFLVLLLIPAWVWYYLKRKKRYASLQVSNTFVFQKQ
                     KLGWKNHLQHLPIVLRSLAFALLVVVLARPQSVDSWRNTTTEGIDIVLALDISTSMLA
                     EDLKPNRLEAAKSIASSFIAGRDNDNIGLVVFAAESFTQCPLTTDHAVLMNLFGSIQS
                     GMIEDGTAIGLGLANAVSRIKESQAKSKVIILLTDGTNNRGEIDPITAAELAKTFGIR
                     VYTIGVGTIGKAPYPFRTANGVVYQNIDVVIDEEPLKQIAGMTGGVYFRATDNTKLQG
                     IYQEIDQLEKTKIEVQEYSKREEEYRP"
     gene            complement(4715..5707)
                     /locus_tag="PLI37_02155"
     CDS             complement(4715..5707)
                     /locus_tag="PLI37_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HOQ57854.1"
                     /translation="MPKRIPILLLFSLLTFPAVLGQDVVVEASLDSSMIWIGNPTTMR
                     LSVSAPRDLSVVFPDFKADTLVRGIQVLKRGPVDTVLLDNDRFRLQKNYLLTSFDAEL
                     YYIPPIEFKVGGVNYESNHLSLKVISFEVDTASMELFDIKGVEKPPFVIWDYALPAGY
                     VLGGLLLLLLGYYLYKRFRKTKQITEVEPDPELLLPPHIVALQALDKIKSEKIWTEGK
                     YKLFYTQVTDVLRKYMGRRFEIPAMELSSSEILDFFRKNKEAQPVYEQLKQLLMLADF
                     VKFAKFQPLESENHQALNHAYGFVENTKAEDSLNTEESEAEPKAGVPVPGEGTV"
     gene            complement(5713..6582)
                     /locus_tag="PLI37_02160"
     CDS             complement(5713..6582)
                     /locus_tag="PLI37_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOQ57855.1"
                     /translation="METSDLLKKVRRIEIKTRGLSRNIFAGQYHTAFKGRGMAFSEVR
                     EYQYGDDVRTIDWNVTARFNHPYIKIFEEERELTVVLLIDVSGSRFFGTESLYKKDMI
                     TEIAATLAFSAMQNNDKVGVIFFSDKMEKFIPPQKGRKHILYIIRELIEFKPENNQTD
                     LSVALQYLTNVIKKRCTAFLISDFLVQQDFQTDLTLANRKHDVVAIQVYDQRETELPS
                     VGLIRVKDAETGREMLIDSASKNLRNNYRRWWEEQELRLDTTFKKSKVDAVSVSTAQD
                     YVKALMALFNLRN"
     gene            complement(6594..>6914)
                     /locus_tag="PLI37_02165"
     CDS             complement(6594..>6914)
                     /locus_tag="PLI37_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOQ57856.1"
                     /translation="QVYMDEKIERYIVDIVFASRYPEEYGLKNLKEMISFGASPRASI
                     NLALAARSYAFIKHRGYVIPEDVRGICLDVMRHRIGLSYEAEANNLSSDEIIQEILNK
                     VEVP"
     assembly_gap    6915..7014
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7015..7415)
                     /locus_tag="PLI37_02170"
     CDS             complement(<7015..7415)
                     /locus_tag="PLI37_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ57857.1"
                     /translation="MSQSVDIRELNELIEKKSSFVDMIVMGMDKVIVGQKHLVESLLI
                     GLLADGHILLEGVPGLAKTLAIKSLASLIKAKYSRIQFTPDLLPADVIGTMIYSQKSE
                     EFAVKKGPIFANFILADEINRAPAKVQSALL"
     gene            complement(7546..8481)
                     /locus_tag="PLI37_02175"
     CDS             complement(7546..8481)
                     /locus_tag="PLI37_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOQ57858.1"
                     /translation="MNNRFLLPDLVRFILDTTEDLDKKEVETFVRAYFTVIEKALFEE
                     DFVKINNLGKFSLQKVEARKSINVNTGEAFEIKAHYKLTFVPDVKLREIANKPFEHLE
                     AIELENEQEPIKKETINLNKPTTKEDNIMEKKEKTVFVVTDKPAANEKKTAPETQKAK
                     KGKLVAWIVFVVVMCALIVFALWKTVFTGPEIEPTITEEVVESAPVAPVVEEVTPPAP
                     KVVDPADWETLKVVPLQKGKRLTLLALEYYGNKVFWVYIYEANRSHIKNPNKILAGTK
                     IRIPKPDPAVINAKDPELVRIAKEKETKYRAQFSK"
     gene            complement(8471..8764)
                     /locus_tag="PLI37_02180"
     CDS             complement(8471..8764)
                     /locus_tag="PLI37_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOQ57859.1"
                     /translation="MTNKEFIRAVQLESKLDKQKTALLVEALNASIGKILSEGDCLNI
                     WGFGSFELKKRGERLSVNPSTGKRFMIPPKLVPVFRPGTNLKNKIKQIDSDEQ"
     gene            complement(8820..11540)
                     /gene="ppdK"
                     /locus_tag="PLI37_02185"
     CDS             complement(8820..11540)
                     /gene="ppdK"
                     /locus_tag="PLI37_02185"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOQ57860.1"
                     /translation="MSKKSVYFFGNKTAEGNGNMRELLGGKGANLAEMNLLGIPVPPG
                     FTISTEICTEYFKKGRDKVVAEIKAEVEAAMKKVEKAVNGPGFGDPENPLLVSVRSGS
                     RASMPGMMDTILNLGMNDQAVEVLSKRSGNPRFAWDSYRRFVQMYGDVVLGMKPEKKE
                     DEDPFEVIIHKMKSQRGVSNDTDLTTDDLKELVSLFKAAVKQQTGQEFPADPWVQLWG
                     AVCSVFDSWMNERAILYRKLNNIPSDWGTAVNVQAMVFGNMGDNSATGVAFTRDAATG
                     EDMFNGEYLINAQGEDVVAGIRTPQQITLEGSRRWAQAQNIDEQERQTKYPSLEETMP
                     EAYKELYAIQEQLEVHTKDMQDIEFTIQDGKLWMLQTRSGKRTGAAMVKIAMDMLRDG
                     MLTEKEALLRIDPEKLDELLHPVFQKSALDAAKPISKGLPASPGAASGRVVFFAEDAE
                     EWADKGEKVILVRTETSPEDLKGMNKAQGILTARGGMTSHAAVVARGMGKCCVSAANN
                     LFIDYKARSMKVGDKSIKEGDWLSLNGSTGYVYEGKLATQDPELSGDFAQLMKLSDKH
                     ARMQVRTNADTPHDAAVARSFGAVGIGLTRTEHMFFEGDKIIAMREMILAGDEAGRRK
                     ALAKLLPLQRADFHGIFEVMHDLPVTVRLLDPPLHEFVPHDEKGQKEMADVMGMTLAQ
                     VKERVESLVEQNPMLGHRGCRLGNTYPEITEMQTRAIIEAALDLKAKGIKVMPEIMVP
                     LTGNYKEFNAQKAVIDETAEVIFLERNARIEYKVGTMIEIPRAALTANRIAESAEFFS
                     FGTNDLTQMTFGYSRDDAASFLPIYLEKGILENDPFAILDQKGVGKLVDMATQKGRDT
                     NPNLKCGICGEHGGEPSSVEFCHRVGLNYVSCSPYRVPIARLAAAQAALR"
     gene            complement(11720..13069)
                     /locus_tag="PLI37_02190"
     CDS             complement(11720..13069)
                     /locus_tag="PLI37_02190"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF006688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOQ57861.1"
                     /translation="MQKCLIKNATLVNEGKVFKASVLIEGQHISRIFTEEAAIPSEVL
                     EKYPVIQAGGCYLLPGVIDTHVHFREPGLTHKADMLSESRAALAGGVTSFFDMPNTIP
                     QTTTLDAWEQKMSLAAEHSMANYAFYLGATNDNLSELIHADYSRIPAVKLFMGASTGN
                     MLVDESSALKELFSSIKAIIAVHAESENIIRQNKERYMAQAGIELPIRYHSLIRDAEA
                     CYRSSAQAVEWASKYGSRLHILHLSTQRELSLLKDLALEEKKITAEVCVHHMWFDDRD
                     YERLGAAIKWNPSVKQETDKLALRTALNCNLIDVVATDHSPHLWSEKEGSCFEAASGA
                     PSVQFGLVLMLELSGRGAFGLDTVVEKMCHAPARLFRIVKRGFIREGYFADLTLLKPG
                     AARKIGKGEIISKCGWSPYEGENVSWHVEKTFVNGSLLYDQGRFSQEKAARALEFSV"
     gene            complement(13070..13819)
                     /locus_tag="PLI37_02195"
     CDS             complement(13070..13819)
                     /locus_tag="PLI37_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOQ57862.1"
                     /translation="MKSDSVVIIPTYNEKENIEAIIREVFDLPQPFHILVVDDGSPDG
                     TASIVKKLQKEFPSALHLEERKGKLGLGTAYIHGFKWALRNGYDYIFEMDADFSHNPK
                     DLLKLYQACSKDGADLAIGSRYINRLVNVVNWPIGRVLMSYYASVYVRIVTGMKVSDT
                     TAGFKCYRRRVLETIELDNIRFKGYAFQIEMKFTTWKHGFRIVEIPIVFTNRELGTSK
                     MSGGIFNEAVFGVIKMRLKSVFKKYPQINRN"
     gene            13963..>15917
                     /locus_tag="PLI37_02200"
     CDS             13963..>15917
                     /locus_tag="PLI37_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOQ57863.1"
                     /translation="MELQDLLTKYGSHTNIKAIAHHLSSSSGILRLNGLAGSATALSL
                     AAVFTGGKRDFLLVQDDAEQAAYLYHDLNQLLGRDRVYLFPSSFRRRIGKGLEDAGNI
                     ILRTEVLNALQFSSREPLIVVSYPEALAEKVLSRQELKNNTLKIKVGEQVDNRFVIEL
                     LHEYGFEQTDYVYEPGQFAQRGSLIDVYSWAYELPFRIDFYGNEIESIRSFEVESQLS
                     KSMFSEVNIILHQQNTKGGHLVSLLDYLDEKTVFGFSGLAYCLNRIELIINDSSAFNA
                     AHLLTEISELTKAIQSNNCLEYGSNTSGEQQAEIKFNCSHQKKFSKRFDLLAEHLQKH
                     INDGYQLCILSNNPKQLERITAIFEERKQKIAYSPILQTLHEGFCDHDLKLLCYTDHQ
                     LFDRFHRYSLRSDRVRNGKLALSMKELLQFQFGDYVVHIDHGVGRFGGLFKTEINGKM
                     QEIIKIIYKDNDTIFVSIHNLHRISKYRGKDGSEPTIHKLGSGAWEKMKNRSKSKIKD
                     IARDLIKLYSQRLQEKGFSYSPDSYLQQALEASFIYEDTPDQLKATAEVKKDMELDKP
                     MDRLICGDVGFGKTEVAIRAAFKAVCDNKQVAVLVPTTVLALQHYQTFSERLKDFPCT
                     VDYISRTRTNAEVKSLLEGLSEGKPDI"
     assembly_gap    15918..16017
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16018..17429
                     /locus_tag="PLI37_02205"
     CDS             <16018..17429
                     /locus_tag="PLI37_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOQ57864.1"
                     /translation="AEVKSLLEGLSEGKPDILIGTHKLVGKNVQFKDLGLLIIDEEQK
                     FGVGVKEKLKQLKVHVDTLTMTATPIPRTLQFSLMGARDLSLMSMPPPNRYPIQTEIY
                     PFDKGVLKEAIEYELERDGQLFFINNRVSQLNELENTIRKLVPGLRTVIAHGQMNGAK
                     LEEIVLDFMNHNYDMLICTSIIENGIDIPNANTIFINNAHQFGLSDLHQLRGRVGRTN
                     RKAFCYLFAPPLELLTPDAKRRLQAIENFSELGSGFNIAMQDLDIRGAGNLLGAEQSG
                     FIADLGFEAYQRILNEAMQELRDEEFPLSSEQEEANRADCRYVSDSLLESDLSLYFAE
                     NYVPGSSERMLLYREMDQLEKEEDIRAFEKRMIDRFGPIPSEGQDLIDVVRLRISAGQ
                     LGMEKIILKNNQMICHFVSNPKSSFYQSSCFDRILNYVQQHYKKTRLREQQGKRSLLI
                     EQVGSVKEAHTILLSMLQS"
     gene            17498..19441
                     /gene="thrS"
                     /locus_tag="PLI37_02210"
     CDS             17498..19441
                     /gene="thrS"
                     /locus_tag="PLI37_02210"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOQ57865.1"
                     /translation="MIQISFPDGSVRSYEKGTTAYQIAESISPRLAKESLAASVNGIT
                     RDLNLPINEDAQVELLKWDEPEAKHAFWHTSSHLLAAALQELYPGIQFGIGPAIENGF
                     YYDVDPGEGVIIKESDLPAIEAKMTALLPDKHVLQRREISKNEALNYFKQRGEHYKVE
                     LIQDLEDGTISTYTLGDFTDLCRGPHLTDTGPIKAFKLTSVAGAYWRGDEKRKQLTRI
                     YGISFPKKAMLDEYLILLEEAKKRDHRKLGRELELFMFSPSVGQGLPMWLPRGTELRS
                     RLEEFLKRIQKKFGYQQVITPHIGQKQLYVTSGHYAKYGKDSFQPIHTPEEGEEFLLK
                     PMNCPHHCEIYRFQPRSYKDLPLRFAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFC
                     RPDQLKDEFLKVVDIIFIIFKALDFQEFEAQISLRDPNNHEKYIGSEENWEKAEQAIV
                     EACAEKNLKVKIVQGEAAFYGPKLDFMVKDAIGRRWQLGTIQVDYNLPERFELEYIGS
                     DNSRHRPVMIHRAPFGSMERFVAVLIEHTGGKFPLWLTPDQVVVLPISERFNDYAYKV
                     ADYLKLQDIRCLVDDRNEKIGRKIRDNELKRIPYMLIVGEKEEENSEVSVRKQGEGDT
                     GSVKMTTFAAIFLDEVEKMMNVI"
     gene            19490..20098
                     /gene="infC"
                     /locus_tag="PLI37_02215"
     CDS             19490..20098
                     /gene="infC"
                     /locus_tag="PLI37_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOQ57866.1"
                     /translation="MKNDDIKFQYRINHRIREKEVRLVGDNVETGVYSIQEALKMAED
                     QRLDLVEISPKAVPPVCRIIDFQKFIYQKKKKEKEQKAKATKITIKEIRFGPQTDDHD
                     YNFKLKHAIEFLKEGSKVKAYVFFKGRSILFKEQGELLLLRFANDLEDYGKVENMPVL
                     EGKRMIIMVAPIKKKAATSSPSKKKSATDKPGKDSGSDKKQE"
     gene            20157..20354
                     /gene="rpmI"
                     /locus_tag="PLI37_02220"
     CDS             20157..20354
                     /gene="rpmI"
                     /locus_tag="PLI37_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOQ57867.1"
                     /translation="MPKIKTNSGSKKRFALTGTGKIKRKHAFKSHILTKKSTKRKRNL
                     TRTGLVDKSDMNNIRLLLGMK"
     gene            20456..20806
                     /gene="rplT"
                     /locus_tag="PLI37_02225"
     CDS             20456..20806
                     /gene="rplT"
                     /locus_tag="PLI37_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOQ57868.1"
                     /translation="MPRSVNHVASRAKRKKILSLTKGYFGSRKNVWTVAKNTWEKGLT
                     YAYRDRKAKKREFRALWIQRINAAARLEGFSYSRLMGALHKAGIEINRKVLADLAMNE
                     PQAFKAIVEKAKNA"
     gene            complement(21270..22529)
                     /locus_tag="PLI37_02230"
     CDS             complement(21270..22529)
                     /locus_tag="PLI37_02230"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOQ57869.1"
                     /translation="MQSIEQFNFSGKKVFVRVDFNVPLDENKNITDDTRIRAAMPTLK
                     KIIKEGGMPIIGSHMGRPKKNPDPKYSLKAIQQHVADCLGAPVHFAEDCQKADAQVAA
                     LKPGEALLLENLRFYAEEEGKPRDLAEDASDEEKAAAKKIVKESQKAFTKKLASYADC
                     YVNDAFGTAHRAHASTALIADYFDDNNKMFGYLMNSEVQAVEKVMRDIVRPFTAIMGG
                     AKVSSKIDIIENLLDKVDNLIITGGMAFTFVKAMGGSIGNSICENDKLDLALELIEKA
                     KQKGVKLVLPIDSKVGDAFSNEANTQFVSIDAIPDGWEGMDIGPKTEALFAEVIENSK
                     TILWNGPAGVFEFENFTHGSKSVGEAVVEATKKGAFSLVGGGDSVACVNKFGLAKGVS
                     YVSTGGGALLEAIEGKVLPGIKAIKGE"
     gene            complement(22599..22913)
                     /gene="rhaM"
                     /locus_tag="PLI37_02235"
     CDS             complement(22599..22913)
                     /gene="rhaM"
                     /locus_tag="PLI37_02235"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOQ57870.1"
                     /translation="MKRVAFKMKMTPGMKEEYIKRHDQIWPEIAELIKKSGVSDYSIF
                     LDEETNILFGVQKVEGNLSSQDLGFNEIQKKWWDYMADIMETNPDNSPITIPLVEVFH
                     LE"
     gene            complement(22954..25047)
                     /locus_tag="PLI37_02240"
     CDS             complement(22954..25047)
                     /locus_tag="PLI37_02240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOQ57871.1"
                     /translation="MNNNKIPILMAIAALSFSCSPKEGDRSKEIPISKPQLELTGDLM
                     TPEILWSMGRVSDIQLSPDKKTVLYGVSYYSITQNKGNRELYLVDMEGKHRRQLTATA
                     VSESAAQWSADGKRIYYLSTVSGSSQLWQMLPDGRKPKKLTSVEGGIDAFRFSPDGKK
                     LLYVAQVKYGKRAVDYYPDLDKTTGRIIDDLMYKHWDEWVETIPHPFVADFSSKGLSN
                     VRDLLQDEPFESPMKPWGGIEHLAWSPDGKIVAYTCRKKTGVEYSRSTDSDIYFCFLE
                     ENFRTENITAELPGYDTNPVFSPCGGSLAFCSMSRDGYESDKNRLMVYELSTGKMKDL
                     TADFDQEANSVVWADDAKSLYFVSCWHGRVHIYQAFTDGSPIRQITGGDYDFADVHVA
                     GEYLVGLRHSLSQANEIIRIDPQSGEMLDLSRENKEIYDQLSLGRVEERWVKTTDNKE
                     MLVWVVYPPHFDSSKKYPALLYCQGGPQSPVSQFWSFRWNLQLMAANGYIVVAPNRRG
                     LPGFGQEWLEQISGDYGGQNMKDYLSAIDALSAEPFVDRDRLGCVGASYGGFSVYWLA
                     GNHEKRFKAFISHSGIFNLEQQYLETEEMWFADWDLGGPYWERDNKTAQLSYANSPHR
                     FVDKWDTPILCIHGEKDYRILASQGMAAFNAAILRGIPAELLVFPDENHWISKPQNAI
                     LWQRRFFAWLDKYLK"
     gene            complement(25161..26021)
                     /locus_tag="PLI37_02245"
     CDS             complement(25161..26021)
                     /locus_tag="PLI37_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ57872.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGREELIKVDEEVELAQRIR
                     NGDRAALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKITKAFSKFEQENERKPSPEELSE
                     ELNIPVDKINDTLKVSGRHISVDAPFVEGEDNNLLDVLINDDSPMADRSLMNESLSLE
                     IDRALSTLTERESEIVKMFFGLGCQEMTLEEIGDKFGLTRERVRQIKEKAIRRLRSTS
                     RSKFLKTYLG"
     gene            complement(26107..27630)
                     /locus_tag="PLI37_02250"
     CDS             complement(26107..27630)
                     /locus_tag="PLI37_02250"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOQ57873.1"
                     /translation="MKKFWRNFLIVLSLVLLSAGVSYLTVSKLSDRLIRPIEQQSINQ
                     VEQPLRLVKAEVVPAIETDFTRAANQTVHAVVHIASKTMRSVGSSGPLDIFEYFFGYR
                     DYNQTPKPQTGYGSGVIISADGYIVTNNHVIEGADEISVTLNDRRNYQAKVIGTDPST
                     DIALIKIEEKGLSFITFGNSDELKVGEWVLAVGNPLNLTSTVTAGIVSAKGRNIGILR
                     GNNQNRVQRDKSYSNLSIESFIQTDAAVNPGNSGGALVNTRGELVGINTAIISSSNAF
                     SGYSFAVPVSIVKKVVTDIKEYGSVQRAILGVDISDITPELSKEKKIDVTEGVYVHAV
                     GERSGALDAGVKEGDIVIAVDGEKVTRAAELQEKISRYRPGDKVRLTVLRDKKELNMN
                     VVLRNLQGSTAIVKEAGIEVLGAAFNELSANEKRQLNISYGIRVGGLTEGKLKEAGVR
                     KGFIILKVNDQRIESVADFEKIVRDVQKNAGFGEAALFIVGIYPSGKVAYYAIDLTQ"
BASE COUNT         7621 a         6509 c         5782 g         7798 t
ORIGIN      
        1 acgactgcgg gccatctcca gattaaatcg cacatcgggg tctcccggat tgagcagcaa
       61 agctctttca taattcaaaa tggccggtgc cacagacccc gatttataat aggcattccc
      121 caggttgtaa tatactttgg ccgaaatacc gtagtttgcc aatacctgct cgtacaatac
      181 tgccgcgtcg tcaaaacggc tttcggaata agcactggca gccaactgca gcacagaatc
      241 ggcctcggta atatctgtat aagttgtcgt tttagcccaa gcctgcaagc caaacaagca
      301 aaagactact aagctgaaaa ggctatttct taacatggac attgtttata gtattttcca
      361 tttgattgat aacttccaaa gttctggtat acaaatcgcc catatgttgg ggttcggtta
      421 ccggggcata acgttcaaat tcgcatgcct gcagaatctg caaaaattca gcacagactt
      481 ctgcgtctac ttttcgccgg ttcaattggg cttcgatgtt atctttgttc aactgagaaa
      541 ccggaatatt gagtttgtcg ctggtataac cccaaagcgc tctcaatact tcatcgagga
      601 attgttcttt gtcctggttt ttcaagtaaa aactggcttt cttgagacgt tttacggcca
      661 ctttattggc tttcctggtc ctgacagcgg ctaagtcggc attggctttg gcctgcttac
      721 gataaatata aagcatcagc aggaaaaaga agaaaggcag tatgtaggcc aaccaaaagg
      781 cagtcttgcc atagataagg ttagtgcggg gacgcaagcg gatattatcc actttgatgt
      841 aacgaatatc ctgacccagc aaacgcaatt gttctctact tactaccgta ccggccacca
      901 ccccttcttt gttttgttcg gaacggccca cctcgagttg aaattcatcg gtagacaaaa
      961 ttttataaga tttatcggtc aggtcaaaat aagaaatatt caccggggga atggtaaagc
     1021 tgccggaata gcggggaatt accaggtatt ctatggtttt ggttccgctg actccccggg
     1081 tagtagtctt aaaattattg gtaaccttgg gatcgtacac gtcaaaatcg gccgggaatt
     1141 tcagctcagg gattctgagc atcttcatat ttccttctcc tctgagttcc aatttgatgg
     1201 tgactgcttc gtcggcttta acctgagtag tgttgatgga agagctcagc ctcaagttgc
     1261 cgctgaaagg attgaagtta gccggtttgc cgaaaggaaa cggctcaacc tgaatccgga
     1321 gcttaggcga tagtatggtt ttccgtactt cctgataaga atcaaaaaag tcgtcaaaca
     1381 aactccgcat cctctgattg gtgtttcgta ttctgaaaat caggtcaaaa cttccgcttt
     1441 caatttcaat ctcgccgctc tgctgtggat aaaggatggt ttgctttaag acggctgttc
     1501 gataattcag accctcgtaa ttttccatat cccactgagt attgggatct atttcaattt
     1561 cctgggccaa aaagcctttg aactcaggaa atttaatatt gttcactccg cttatatcta
     1621 tccgggtata caatttataa gtcaacaaca aaccttcttg ttcaaagact ttagttttgg
     1681 aagggatcgc tctgataaat gcctgttcgc cattgaaacc gactgtttgt tgcgaggaag
     1741 ttccctggtc tgaagctccg cctgtacttt tatcctgagg aaggaccgta atagtaagcg
     1801 gattggagct ccttttttct ttttttatgg tcgcagtggc cgcgggaata gtaaaagacc
     1861 ccacttttac gggcatcaaa acataggtat agctgtgttc gaccagagag gtccgctgac
     1921 cgttgactat gcttatcttg gtagatttcg cggtactggg tcccatcaat acctcaaaat
     1981 cggtgatttc gggtattctg aggtctttgg tttcggcatt cagggtgtaa gtaagcctaa
     2041 actgttgacc cagaatgacc tttgaaggag ctgttgccgt aaaaacaact tcatcggcag
     2101 ccgccagctc cataccgaaa atcatcaata tggttgtaaa cgctattcct atatatctac
     2161 ttttcataca tacaccgtaa tcttaccgta ctcaggaaac aaaggtagtc tttttaccaa
     2221 tccttttctg ttttcttcgg ccggctattc tgtatttgta atttcttcac tttttcctgt
     2281 acctcctgtt cgtcctgcat cagggcatcc aacaattgct gcgccttttc acggcttatg
     2341 tgttcttgct gttgttcttg ctgatcgggc tgattttgct gctcgggtct ttcctgttcc
     2401 tgctgttcct gctgctcttg ttgttcctgc ttttgctctt cttcttgttg ctgttgttcc
     2461 tgctgtggtt gttcctgctg tttttttaac atagcttgag ccatggccag attatagcgg
     2521 gtctgatcgt ccttataatt atgaatcaat gcctgcttgt aagcctcaat ggctttcccg
     2581 aaatcctgag cctgcataaa agaattgcct atattatgcc aggcagacga caatcgcata
     2641 gaatctttct cggtgccggt aatctgctgg tactgctcta tggcttcttt aggcttttct
     2701 tgcttaaaca gagagtttcc caaattgaag atgccttcgg gcgattgcct gtccacctcc
     2761 aaagacttgc gatattcaat ttcggcttcg gtgaattttt cctcttcata aagactattc
     2821 ccctggcgaa tatgtttcct ttctgccttt tgagcaaaac cagccagacc gaacaaaaac
     2881 aagaacacaa atataccgat gcttcgcatg tcaaaagagt tttaaattac gtaaagccgg
     2941 atttttttta tccagaataa acatatccac aaacaaaagc aataaaacta tccaggccag
     3001 agcttgaaat tgctcatcgt attcagcata aacggtggtt tcgatatcgg actttttcat
     3061 cgagtcgagc tctttgctca acaccctgag cgccgtgttg gtgttgtcgg cacgaacata
     3121 tacccctccg ccggccattg ctatttgttg acacatttct tcgttgagct tagtaatgac
     3181 cacattaccc tctttgtctt tgtgaaagtc gttcaaaccc cggggaatag gggctccctt
     3241 gagagatccc ataccaacca aatgaacttt aacaccctgt ttttgagctt cctgagcctg
     3301 caaaacagca tcgtcttcgt ggttttcgcc gtcggtaatt accacgatgg ctcgttccgc
     3361 ttcctgggcc aacccaaaag aacgtaaagc cagctgaata gctgctccta tagccgtacc
     3421 ctgagttgga accatctccg tggaaatagt attcaaaaac attttggccg aaatgtaatc
     3481 ggccgtaatg ggtaattgag tataggcctc tcccgcaaaa acaatcaaac ctatcctatc
     3541 gttggccaac tgatctatca agcgggagag agtttgtttg gacttttcga ggcgattggg
     3601 tactaaatct tcggccatca ttgaattgga aacatccagt gcaacaataa cctcggcccc
     3661 ttgtctcttt tgtttttcca atttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnggtctg tattcctcct cgcgtttact gtattcctgt acttctattt tggttttttc
     3841 caattgatca atttcctgat agattccttg caatttagtg ttgtctgtag cccggaaata
     3901 tactcctccg gtcatgccgg caatttgttt gaggggttcc tcatcaatga ccacatcaat
     3961 attttgatat accactccgt tggcggttct aaaaggataa ggagctttgc ctatggtccc
     4021 tacccctatg gtgtataccc gtattccgaa ggttttggcc aattctgccg ctgtgatcgg
     4081 atcgatttcg ccccggttgt tggtgccatc ggtcaacaaa ataataacct tggatttagc
     4141 ctgagattct ttgattctgc ttacggcatt ggccaatccc aatcctatag ccgtaccgtc
     4201 ttctatcatg ccgctttgaa tgctcccaaa gaggttcatc aacacagcat gatccgtagt
     4261 caagggacat tgagtaaaac tttcggcggc aaacacaacc aggccgatgt tatcgttatc
     4321 cctgcccgct ataaacgaag aagcaatact tttggccgcc tccaggcgat tgggtttaag
     4381 gtcttcggcc agcatactgg tcgaaatatc cagtgccagt acaatatcga taccttccgt
     4441 agtggtattc cgccaactgt ctaccgattg agggcgggcc agcactacaa ccaatagagc
     4501 aaaagcaagg ctgcgcaaaa ctataggtaa atgctgaagg tggtttttcc aaccgagttt
     4561 ttgcttttga aacacaaaag tattggatac ctgcaaactg gcgtatcgtt tttttcgctt
     4621 caggtaatac catacccagg caggtataag caaaagtaca aggaacaaat aatagggctg
     4681 cgcaaatatc atactataaa catctgtaaa atcattaaac ggtgccttca cccggcaccg
     4741 ggacgcctgc tttaggttcc gcttcggatt cttccgtatt caagctgtct tcagccttgg
     4801 tattctccac aaaaccataa gcatggttca aggcctgatg gttttcagac tccaagggtt
     4861 gaaacttggc aaacttaaca aaatcagcca gcatcaataa ttgtttcaat tgttcgtaaa
     4921 caggctgagc ctctttattt tttctgaaaa aatccaaaat ttcggagctg ctgagttcca
     4981 tggccggaat ttcaaagcgc cttcccatgt atttacgcag gacatcggtt acctgagtat
     5041 aaaataattt atacttaccc tctgtccaaa ttttttcgct tttgatttta tccaaagcct
     5101 gcaaggctac tatatggggc ggcaacaaaa gttccggatc gggttcgacc tccgtgattt
     5161 gtttggtttt gcggaatcgt ttatacaaat aatagcccag caacaacaat aaaagccctc
     5221 ccagcacgta accggccggc aaagcataat cccagataac aaaagggggt ttttctactc
     5281 cttttatatc gaacagctcc atgctggccg tatccacctc aaaactgatt accttgagcg
     5341 acaagtggtt gctctcataa ttaactcctc ctactttaaa ttctatggga ggaatataat
     5401 acaattcagc atcaaaagag gtcaacaaat aatttttttg caatctaaac cgatcgttgt
     5461 ccaataaaac agtatccacc ggacctcgct ttagaacttg tattccccga acaagcgtgt
     5521 ctgccttgaa atccggaaat actaccgaaa gatcacgggg tgctgaaaca ctcagacgca
     5581 tcgttgtagg attacctatc cagatcatag aagagtccag gctggcttcc accactacgt
     5641 cctgtcccag gacagcagga aatgtcaata agctaaacag caataagatt ggtattcttt
     5701 tgggcatagc gatcaatttc tcagattgaa caaggccatc agggccttca cgtaatcctg
     5761 agcagtagaa accgaaacag catctacttt actctttttg aaagttgtat ccagacgcag
     5821 ctcttgttct tcccaccagc ggcggtaatt atttcgcaga tttttggaag cgctgtcaat
     5881 caacatttcc ctgcctgttt ctgcgtcttt aaccctgatc aaacccaccg aaggcaattc
     5941 ggtctcccgc tgatcgtaga cctgtatggc taccacgtcg tgcttacgat ttgccagagt
     6001 caggtcggtt tgaaagtctt gctgaaccag gaaatcggaa attaaaaaag ccgtacaacg
     6061 cttcttgatg acattggtca agtattgcaa agctacgctc aggtcggttt ggttgttttc
     6121 aggtttaaat tctatcaatt cacggataat atataaaata tgcttcctgc ctttttgggg
     6181 aggaataaat ttttccatct tatccgaaaa aaagatcacc cctaccttat cgttattctg
     6241 catggccgaa aaagccagag tagcagcgat ttcggtaatc atatcttttt tatacaggga
     6301 ttccgtgcca aaaaaacgac tacccgacac atctataagt aacacgacag tcagttcccg
     6361 ttcttcttca aaaatcttaa tgtaagggtg gttaaatcgc gccgtgacat tccagtctat
     6421 agtgcggaca tcatcgccat actgatattc acgcacttcg gagaacgcca tgcctcgtcc
     6481 cttgaaagcc gtatgatact gaccggcaaa aatattacgt gaaaggccac gagttttgat
     6541 ttctattctt ctaacctttt ttagtaagtc actcgtttcc atacttcaga acattaaggc
     6601 acctcgactt tattcagaat ctcctgaata atttcatcac tactcagatt gttggcctct
     6661 gcttcgtaac tcaggccaat acggtgacgc attacatcca ggcaaatacc gcgcacgtct
     6721 tcgggaataa cataaccacg gtgtttgata aaggcatagc tgcgggcagc cagagccagg
     6781 ttgatactgg cacgcggaga agcgccgaat gaaatcattt ctttgagatt tttaagtcca
     6841 tactcctcag ggtaacggct ggcgaatact atatcaacta tgtagcgttc aattttttca
     6901 tccatataaa cctgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntctagc
     7021 aaagcacttt gtaccttggc cggagctctg tttatttcgt cagccagaat aaaattagcg
     7081 aatatcgggc cctttttaac agcaaattct tcggatttct gactgtaaat cattgttcca
     7141 atcacgtcgg ccggcaaaag atcgggagta aactgaatcc ggctgtattt ggctttaata
     7201 agtgaagcca gacttttaat ggccaaggtc tttgccaaac cgggcacacc ttccagcaat
     7261 atgtgtccgt cggccagcag gcctatcagc agggattcaa ctaaatgttt ttgtccgaca
     7321 atcaccttgt ccatacccat gacaatcatg tcgacaaaac tgctcttctt ttcgatcagt
     7381 tcatttaact ctcgaatatc aacgctttga ctcatatcta taaaactttt aaattttgat
     7441 atttgttaga tggaacacaa aattataaag ataggtttag ttttaaggag cttagccgtt
     7501 aaaaaaacct atctttatag acaaaattcg ttatttatac gaattttact tagaaaattg
     7561 cgctctgtat tttgtttctt tttctttggc aatacgtact aactcgggat ctttagcgtt
     7621 tattacggcc ggatcaggtt tagggatacg aatcttggtt ccggcaagaa tcttgttggg
     7681 attcttgata tgtgacctgt tcgcttcgta aatataaacc caaaaaactt tatttccgta
     7741 gtattccaag gcaagtaaag taaggcgctt tcctttttgc agaggaacca ctttgagtgt
     7801 ttcccaatcg gccggatcga ccactttggg agcaggcgga gtgacttcct ccactaccgg
     7861 cgcaacggga gcgctctcga ctacttcttc tgtaatagtc ggttcgattt cgggcccggt
     7921 aaatacagtc ttccataacg caaagacgat cagtgcgcac atgacgacaa caaaaacaat
     7981 ccaggcaacc agtttgcctt ttttggcctt ctgtgtttca ggtgccgttt ttttctcgtt
     8041 tgcagccggt ttatccgtta ctacaaaaac agttttttct ttcttttcca tgatgttgtc
     8101 ttcttttgtg gttggtttat tgaggttgat tgtttctttt tttatcggtt cctgttcatt
     8161 ttctaattct atggcttcca gatgctcaaa tggtttattg gctatttccc tgagtttaac
     8221 atcgggcaca aaggtcaact tgtaatgagc cttaatttca aaagcttctc ccgtgttaac
     8281 attgatactt ttgcgcgcct ctactttctg caggctgaat ttacccaaat tgtttatttt
     8341 aacgaaatct tcttcaaaca aagccttttc tataacggta aaataagccc gtacaaaagt
     8401 ttccacttct tttttatcca gatcctccgt cgtgtcgaga atgaaccgga ccaaatccgg
     8461 aagtaaaaat ctattgttca tctgagtcta tttgtttgat tttgttcttc aggttggttc
     8521 ccggcctgaa aaccggaacc aatttaggag gaatcataaa ccgttttccg gtagatggat
     8581 tgaccgaaag acgttcgcct ctttttttga gttcaaaaga accgaagccc catatgttca
     8641 ggcaatcacc ttcagacagt attttgccga tggaagcatt gagagcttcc accagcaaag
     8701 ctgttttttg tttatccagt ttggattcaa gctgaacagc acgaataaat tctttgtttg
     8761 tcatagtatt ttatcgattt ttttcaaatc aaattctcgt actgaacaga aaccgttcct
     8821 tagcggagag cggcctgcgc agcagccaaa cgggcaatag gcaccctata gggagagcaa
     8881 cttacatagt tcaatcccac acgatggcaa aattccaccg acgagggttc tccaccatgc
     8941 tcaccgcata ttccacattt cagattggga tttgtgtctc ttcctttttg agttgccata
     9001 tcgaccaatt ttcctacccc tttctgatcc agtatagcaa aggggtcgtt ttccaatatt
     9061 cctttttcca agtaaatggg aaggaaagaa gccgcatcgt cgcgactata cccaaaggtc
     9121 atttgagtca ggtcgttggt tccaaatgaa aagaattctg ccgactcggc aatgcgatta
     9181 gctgttaaag cagccctcgg aatttcgatc atggtaccaa ccttgtactc tatgcgtgca
     9241 ttgcgttcca ggaaaatgac ttctgctgtt tcgtctataa cggctttttg agcattgaat
     9301 tccttgtagt tgccagtaag aggcaccatg atttcgggca tgactttgat gccttttgct
     9361 ttgagatcca gcgcagcctc aataatggcc cgggtttgca tttcggtaat ttcaggatac
     9421 gtattaccca aacggcaacc tctgtgaccc aacatgggat tttgttctac caacgattca
     9481 acacgttctt tgacttgagc caaggtcata cccatgacat cagccatttc tttttgtcct
     9541 ttctcgtcgt gaggaacaaa ttcgtgcagg ggaggatcca acagacggac agttaccggc
     9601 aaatcgtgca tcacttcgaa aataccgtga aagtcggctc tttgtaaagg taagagttta
     9661 gccagcgctt tacgtctgcc tgcttcgtca ccggcaagga tcatttcgcg catggctatg
     9721 attttgtcgc cctcaaaaaa catatgttcc gtacgggtaa gccctatacc aacggccccg
     9781 aaagatctgg ccacagctgc atcgtgcggg gtgtcggcat tggttcttac ttgcatgcgt
     9841 gcatgtttat cgcttagctt cattaactga gcaaaatcac cgctcagttc aggatcctgg
     9901 gtagccagtt tgccttcgta aacataaccg gtagatccgt ttaacgacag ccaatctccc
     9961 tcttttatgc ttttatcgcc cactttcata ctgcgggctt tgtagtcaat aaacaggtta
    10021 ttggcggctg atacacagca tttacccatc ccacgggcta ccacagccgc atgagaagtc
    10081 atgccgccac gggctgttaa aatgccctga gctttattca ttcctttcag gtcttcgggc
    10141 gaggtttctg tgcgaaccaa aataaccttt tctcctttgt ctgcccattc ttcggcatct
    10201 tctgcaaaaa agaccacacg gcccgaagct gctcccggtg aggccggcaa accctttgat
    10261 atcggcttgg cggcatccag cgctgatttt tggaaaacag gatgcaaaag ttcgtccagt
    10321 ttttcgggat ctatccgcaa aagggcctct ttttctgtca gcatgccatc acgcagcata
    10381 tccatggcga tttttaccat ggcagctccg gtacgtttac cgctgcgggt ctgcagcatc
    10441 cataatttac cgtcttgtat ggtaaattca atgtcctgca tgtccttagt gtgtacttcc
    10501 aactgttcct gaatggcata cagctctttg taggcttcgg gcattgtttc ttccagtgaa
    10561 ggatacttgg tttggcgttc ctgttcatca atattctgag cttgtgccca acgcctggaa
    10621 ccttcaaggg tgatttgctg tggggtgcga attccggcta ccacgtcttc gccctgtgcg
    10681 ttgataaggt attcgccgtt aaacatatct tcgccggtag ctgcgtctct ggtaaaagcc
    10741 acaccggtgg ccgagttatc gcccatattg ccaaaaacca tggcttgcac gtttacggca
    10801 gttccccaat ctgaaggaat gttattaagc ttgcgataca agatggcccg ttcgttcatc
    10861 caactgtcaa atactgaaca gacggcaccc caaagttgca cccaggggtc tgcgggaaat
    10921 tcctgacccg tctgttgctt tacggcggcc ttaaacaaag aaaccagttc tttgaggtcg
    10981 tcggtagtga gatcggtatc gttggaaacc ccgcgttggg atttcatttt atgaataatc
    11041 acttcaaaag gatcctcgtc ttctttcttt tcgggtttca tacccaatac cacatcgccg
    11101 tacatttgta caaacctgcg gtaagaatcc caggcaaaac gaggattgcc tgatcttttt
    11161 gacaagactt ccacagcctg gtcgttcatt cccagattca ggatggtatc catcatccca
    11221 ggcatggaag ctctggatcc ggagcgtact gaaaccaaca gaggattttc gggatctcca
    11281 aaaccggggc cgtttacagc tttttccact tttttcatag ccgcttcaac ctccgctttt
    11341 atttcggcta ctactttatc tctgcctttt ttgaaatact cggtacatat ttcggtacta
    11401 atggtaaatc ccggaggaac aggtataccc agcaaattca tctcggccag gttggcacct
    11461 ttacctccta acagctcgcg catatttccg ttgccttcgg ctgttttatt tccaaaaaaa
    11521 taaacgctct tcttgctcat tgtaaaatta ttgtaaaaat tagtagttgt tatagctgta
    11581 acagctctat tttagtatct atcaggctgc aaaggtaaat gtttctttag atatgaacaa
    11641 acaaaaaccc aaattgaccc tgatataaat acagcccatg taccaaaaaa catgtttgtt
    11701 tcaacatccg gtctatgtct cagacactaa attccaaggc tcgggccgct ttttcctgac
    11761 taaaacgacc ctggtcatac agcagggaac cgtttacaaa agttttctcc acatgccagc
    11821 ttacattttc cccctcataa ggcgaccacc cgcatttact gatgatttcc cctttgccga
    11881 ttttgcgggc tgcaccgggt ttaagcagag tcaaatcggc aaaataacct tcgcgaataa
    11941 agcctcgctt aacaattcga aacaagcggg ccggcgcatg acacatcttt tcaaccacag
    12001 tatccaggcc gaaagcacct ctgccggaaa gctccagcat caagactaac ccaaactgaa
    12061 cagaaggagc tcccgatgcc gcttcaaagc aggaaccttc tttttcggac cagagatgcg
    12121 gggaatgatc tgtggccacc acatcaatca aattgcagtt taaggcagtt ctaagggcca
    12181 gcttgtctgt ctcctgttta acggaaggat tccacttgat ggcagcaccc agtcgttcat
    12241 aatcccggtc gtcgaaccac atatgatgca cgcagacttc agcggtaatc tttttttctt
    12301 ccagggccag gtccttgagc aagctgagct cacgttgggt agaaagatgt aaaatatgca
    12361 ggcggctgcc gtatttgctt gcccattcta cggcctgggc agaagaacgg taacaagctt
    12421 cggcatccct tatcagcgaa tggtaacgaa tgggaagctc gattcccgcc tgagccatat
    12481 agcgttcttt gttttgtctg ataatattct ccgattcggc gtggacggca attattgctt
    12541 taatgcttga aaagagctct tttaacgccg aagattcatc gaccagcata ttaccggtcg
    12601 aagctcccat aaaaagcttc actgcgggaa tgcggctgta atctgcatga ataagttcag
    12661 ataaattatc attggtggca ccaaggtaaa atgcgtaatt agccatggaa tgttccgcgg
    12721 ccaaactcat tttttgttcc caggcatcca gagtggttgt ctgggggatg gtgttgggca
    12781 tatcaaaaaa agacgtaacc cctcccgcca acgcggcgcg cgactccgac aacatgtcgg
    12841 ctttgtgggt gagtcccggt tcccgaaaat gtacatgagt gtctatgacg cccggcaata
    12901 aataacagcc ccctgcctgt atgacgggat atttttccag cacctcagaa ggtatggcgg
    12961 cctcttcggt aaatattctg cttatgtgtt gtccttcgat taaaacagat gccttgaata
    13021 ctttaccctc gttaaccaag gtggcattct taatcaagca cttttgcatt tagttcctgt
    13081 ttatttgagg gtatttttta aaaacacttt tcagtcgcat tttgattaca ccaaaaacag
    13141 cttcgttgaa tataccacca ctcattttag aagtgccgag ctcccggttg gtaaacacta
    13201 tgggaatctc aacaatacga aatccgtgtt tccaggtcgt aaacttcatt tctatctgga
    13261 aagcatagcc tttgaaacga atgttatcga gttcaatggt ttctaatacc cggcgacgat
    13321 agcatttaaa accggccgtg gtatcggaca ccttcattcc ggtaacgatg cgtacgtata
    13381 cggaagcata gtacgacatc aacacccttc ctataggcca gttgaccaca tttaccaacc
    13441 tgtttatata gcgtgagccg atagccaaat ccgctccgtc tttggagcag gcttggtata
    13501 atttgagtaa atccttggga ttgtgtgaaa aatcggcatc catttcaaaa atataatcgt
    13561 agccgttgcg caaagcccat ttaaagccat gaatataagc cgtccccagg ccaagctttc
    13621 cttttctctc ttccagatga agagctgaag gaaattcctt ctgcaatttt tttacaatgg
    13681 aagctgtacc atcgggagaa ccgtcatcga caaccaaaat atgaaagggc tgaggtaaat
    13741 caaaaacttc gcgtatgatg gcttcaatgt tttctttttc gttgtaggtg ggaataatga
    13801 cgactgaatc tgatttcatt gtacgaaatt tgtgtttaat ttctgctgat taatattact
    13861 ttattaggct cgataaataa accaccaaat atacggtttt tttgcttctt cctgctgtat
    13921 tggccgtatt ttttataatt ttgcaggatt ttaattccca aattggaact acaggactta
    13981 cttacaaaat acggctcaca taccaacatt aaagcgattg cccatcatct gagctcatct
    14041 tcaggcatac tgcgactcaa tggcctggca ggttcggcta cggctctgtc actggctgcg
    14101 gtttttaccg gcgggaaacg tgattttctg cttgtacagg acgatgccga acaagctgct
    14161 tacctttacc acgatctgaa tcaattgctg ggccgggaca gggtgtattt attcccctca
    14221 agtttccggc gcagaatcgg aaaaggtttg gaagacgccg gcaatattat tctcagaacc
    14281 gaagtcctga atgctttaca gttttcttcc agagagcctc tgattgtagt cagttatccc
    14341 gaagccctgg ccgagaaagt cctctcacgt caagagctca aaaacaatac tcttaaaatc
    14401 aaagtcggag agcaagtcga caaccgcttt gtaatcgaat tgctccatga gtatggtttt
    14461 gaacaaaccg attacgttta tgagccgggg caatttgcac aaagagggag tttgatagat
    14521 gtctattcat gggcttatga actacctttt cgtatcgatt tctacgggaa tgaaatcgaa
    14581 agcattcgca gctttgaggt agaaagccaa ttgtccaaat ccatgttttc ggaggttaac
    14641 atcatacttc atcagcaaaa caccaaagga ggacacttag tttctctact tgattacctg
    14701 gacgaaaaaa ctgttttcgg tttttcgggg ctggcgtact gcctcaatag aatagaattg
    14761 ataatcaatg actcttctgc ttttaatgca gctcatttat tgacggagat cagcgaactg
    14821 acaaaagcta tccaatcaaa caattgcctc gaatatggca gcaacaccag tggtgaacaa
    14881 caggccgaaa ttaaattcaa ctgcagtcat caaaaaaaat tttccaaacg ctttgactta
    14941 ctggccgagc atttacaaaa acacataaac gatggctatc aattgtgcat tttgagcaat
    15001 aaccccaaac aattggaacg catcacggct attttcgaag aaagaaagca aaaaatagcc
    15061 tatagcccta tattgcaaac cctgcacgag ggcttttgcg atcacgatct caaattgctt
    15121 tgttataccg atcatcagct gtttgaccgc tttcataggt acagtttgcg ttccgaccgg
    15181 gtgcgcaacg gaaaactggc cttgagcatg aaggaattgc ttcaattcca attcggcgat
    15241 tacgtggtgc acatcgatca cggtgtggga cgtttcggcg gtttgttcaa gacggaaatc
    15301 aacgggaaaa tgcaggaaat catcaaaatt atttacaaag acaacgatac catctttgta
    15361 agcatccaca acctgcatcg tatttccaaa taccggggca aagatggtag cgagccgacc
    15421 atacacaagc tgggcagcgg ggcctgggaa aagatgaaaa accggagcaa gagcaaaata
    15481 aaagacattg cccgcgacct tattaagttg tattctcagc gcctgcagga gaaaggtttt
    15541 tcctacagcc ccgattccta tctgcaacag gctctggaag cctcctttat ttacgaggat
    15601 acccccgatc agctcaaagc cacggccgaa gtcaaaaaag acatggaact ggataagcca
    15661 atggacaggc tgatttgcgg cgatgtcgga ttcggaaaaa cggaggtggc catacgggct
    15721 gccttcaaag ctgtctgcga caacaaacag gtcgcagtac tggttccaac cacagtgctg
    15781 gctctccaac attatcaaac cttcagcgaa agactcaaag attttccctg cacggtcgat
    15841 tacatcagca gaaccagaac aaatgccgaa gtaaaaagct tgcttgaagg tcttagtgaa
    15901 gggaaaccgg atattttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg
    16021 ccgaagtaaa aagcttgctt gaaggtctta gtgaagggaa accggatatt ttgatcggta
    16081 cacacaagct ggtaggaaaa aacgtccaat tcaaggatct gggattattg attatcgacg
    16141 aggaacaaaa attcggtgtc ggtgtgaaag aaaaactcaa acagctcaaa gtccacgttg
    16201 acacattaac catgaccgcc acccccattc ccagaacctt acagttttct ctgatgggtg
    16261 cccgcgattt gtcgctgatg agcatgcctc cccccaatcg ttacccgata caaaccgaaa
    16321 tttacccctt cgacaaaggg gtgcttaaag aagccataga atacgaattg gaacgtgacg
    16381 gacagctttt ttttatcaac aaccgggtta gtcagctaaa cgaattggag aacaccatac
    16441 gcaaactggt tcccggcttg cgtaccgtga ttgcacacgg ccagatgaac ggagccaaac
    16501 tggaagaaat cgttctagac tttatgaatc ataattacga tatgctcatt tgcaccagca
    16561 tcatcgaaaa tggaatagat attcccaatg ccaacactat ttttataaac aatgctcatc
    16621 agtttggcct gagtgacttg caccaactca gaggacgtgt gggccgtacc aatcgtaaag
    16681 ctttctgtta tctctttgcc cctcccctgg aattgcttac ccccgacgcc aaacgccgtc
    16741 tgcaagccat agagaacttc agcgaactgg gcagcggctt caacattgcc atgcaagacc
    16801 tcgacatcag aggtgccgga aatttgctgg gagctgaaca aagcggcttt attgccgatc
    16861 tgggttttga agcctatcag cgcattctga atgaggctat gcaggaactc agggacgaag
    16921 aattccctct cagttcagaa caggaagaag ccaaccgggc agattgccgt tatgtaagcg
    16981 atagcttgct cgaatcggac ctctccttat attttgccga aaattacgta ccaggctcgt
    17041 cggaacgcat gttgctttat cgcgaaatgg atcagctcga aaaagaagaa gacatccggg
    17101 ctttcgaaaa acgaatgatc gaccgtttcg ggcctattcc cagtgaaggc caggatttaa
    17161 tcgacgtagt gcgtttgcgt atctcggccg ggcaactggg catggaaaaa attattctta
    17221 aaaacaatca gatgatttgt catttcgtat ccaacccgaa atcctctttt tatcagtcca
    17281 gttgttttga ccgcattctg aattatgttc agcagcatta caaaaagacc aggttgcgtg
    17341 aacaacaagg taaaagatcc ctgctcattg agcaagtagg cagcgtaaag gaagcccata
    17401 ccatacttct ttcgatgctt cagtcataaa aagaagcatt aaagcaactt gaggaattta
    17461 ttttttgtac ttttgtgcgt tttttaatac acaaattatg attcaaatca gctttccgga
    17521 tggatccgtt cgctcgtatg aaaaaggaac caccgcttat caaatagcag aaagcatcag
    17581 tccccggctg gccaaagaaa gcctggccgc ctccgtcaac ggtattaccc gcgatctgaa
    17641 tcttcccatc aacgaagacg ctcaggtgga attgttaaaa tgggatgaac ccgaagccaa
    17701 gcacgctttc tggcacactt cttctcatct gctggctgcc gccctacaag aattgtatcc
    17761 gggcatccag ttcggtatag gacctgccat cgaaaacggt ttttattacg acgtcgatcc
    17821 cggcgaggga gtcattatta aagaaagcga tttgccggcc atcgaagcca aaatgacagc
    17881 tttactgccc gacaaacacg tactgcaacg ccgggagata tccaaaaacg aagctctgaa
    17941 ctatttcaaa caaagaggcg aacattataa agttgaactt atccaggatt tggaagacgg
    18001 cactatcagc acctatacct taggtgattt caccgatttg tgccgcggcc ctcacttaac
    18061 agacaccggc cccatcaagg ccttcaagct gacatccgta gccggcgcct attggagagg
    18121 cgacgaaaaa cgcaagcaac tcacccgcat ctacggcatc agtttcccca aaaaagccat
    18181 gctcgatgaa tacctgatct tgcttgaaga agcaaaaaaa cgtgatcatc gcaagcttgg
    18241 ccgcgagctg gaattgttta tgttttcacc ttctgtgggg caaggtttac ccatgtggct
    18301 gccccgtggc accgaattgc gcagcaggct cgaagaattt ctgaaacgga ttcaaaagaa
    18361 attcggttat cagcaagtca tcaccccgca tatagggcaa aaacagcttt atgtaacttc
    18421 gggtcattac gccaaatacg gcaaggattc gttccagccc atacacaccc ccgaagaagg
    18481 cgaagaattt ttgcttaaac cgatgaattg cccccatcat tgcgaaatct accgctttca
    18541 gccccgttct tacaaagatc tgcccctgcg ttttgctgaa tttggcacgg tctatcgtta
    18601 tgaacaaagc ggagaattgc acggtctgac cagggtaaga ggttttactc aagacgacgc
    18661 ccatattttc tgtcgtcccg atcaactcaa ggatgaattt ctcaaggtag tggacattat
    18721 ttttatcatc tttaaagcgc tggattttca ggaatttgaa gctcaaattt ctctgcgcga
    18781 tccaaacaat cacgaaaaat acatcggtag cgaagaaaac tgggaaaaag cagagcaagc
    18841 catcgtggaa gcctgtgccg aaaagaacct gaaagtgaag atagtccagg gagaggctgc
    18901 tttttacggc cccaagctgg acttcatggt caaggacgcc atcggaagac gctggcaact
    18961 gggaaccatt caggtagatt acaaccttcc cgaacgtttc gaactggaat acatcggcag
    19021 cgacaacagc agacatcgcc cggtaatgat acatcgtgcg ccttttggat caatggaacg
    19081 ctttgtggcc gtgcttattg aacatacggg aggtaaattt cccctctggc tgactcccga
    19141 ccaggtggtg gtgcttccca taagcgagcg tttcaacgat tatgcctaca aagtggccga
    19201 ctatctcaag cttcaggaca tccgttgtct ggtagacgac cggaacgaaa aaatcggccg
    19261 gaaaattcgc gacaatgaac ttaaaagaat accttacatg ctgattgtcg gggaaaaaga
    19321 agaagaaaat tcagaagttt ccgtaagaaa acaaggcgaa ggtgatacag gttcggttaa
    19381 aatgactacc tttgcagcga tttttttgga tgaagtagag aaaatgatga acgtaatata
    19441 gttaagtttc taaaaattaa taatatacaa cacagggaca aacatttgaa tgaaaaacga
    19501 cgatattaaa tttcaatacc ggattaacca cagaattcgt gagaaagaag ttcgattagt
    19561 aggcgataat gtggaaacag gcgtttacag cattcaagaa gcattaaaaa tggccgaaga
    19621 tcaacgattg gatttggtgg aaatttcacc taaggccgtt cccccggttt gtcgtattat
    19681 tgattttcag aagtttatat atcaaaagaa aaaaaaggaa aaagaacaga aagccaaagc
    19741 aactaagatt accatcaaag aaattcgctt cggtccgcaa accgatgacc acgactacaa
    19801 ttttaaattg aagcatgcca tcgaattctt aaaagaaggt tccaaagtca aggcttatgt
    19861 atttttcaag ggacgttcca ttttgttcaa ggaacaggga gagctcttat tattgcgctt
    19921 tgccaacgac ctggaagact atggaaaagt ggagaatatg ccggtactgg aaggcaaaag
    19981 gatgattatt atggtggctc ctatcaaaaa gaaagctgca acctcctctc cttccaaaaa
    20041 gaaatcggca acagataagc ctggaaaaga ttccggtagt gataaaaaac aagaataaaa
    20101 gaccgtgcga acgggctgta atacaatcaa ttaaaaacag tttaaagtaa aacaaaatgc
    20161 caaagatcaa gactaactcc ggttcaaaaa agaggtttgc ccttaccgga acaggtaaaa
    20221 tcaaaaggaa acatgcattt aagagtcata ttctgaccaa gaaatcaacc aagcggaaac
    20281 gcaatctcac ccgaaccggg ctggtagata aaagcgacat gaacaacatt cgtcttttgt
    20341 tgggtatgaa gtaatgctat tcctacattg tttattactt ataaattaac cgaatgcttt
    20401 agcccataag atcatctgag aagaatgacg ctaacattca aaaaaaatta ctacaatgcc
    20461 aagatcagta aatcacgtag cttcaagagc aaaaagaaag aagattctat ccctgaccaa
    20521 agggtatttc ggatccagaa aaaacgtttg gaccgtagct aaaaatactt gggaaaaagg
    20581 tctcacctat gcctatcgcg accgcaaagc caaaaaacgc gaatttcgtg cattatggat
    20641 acagcgcatc aacgccgctg ccagactgga aggtttttca tacagccgcc tgatgggtgc
    20701 tttgcacaaa gccggcatcg aaatcaatcg taaagttctc gccgatttgg ccatgaatga
    20761 accccaagcc tttaaagcca tcgttgaaaa ggccaaaaac gcttaagaag aaagaaaaat
    20821 aaagcgatac ccataaaaaa gtcccgaaaa cataaagttt tgggactttt ttgatgcaaa
    20881 atcaattaat tctcttatat ttgtgtcacg gaagtcgtgt cgatttgatt tggtaaactc
    20941 atcaatttaa aaaagaaaca ccgagactca gcttaggttt ttcaatggcg ggccacactt
    21001 ctcaggaagc gcttttcgaa gcaaggtgga ttacaaagaa gaccggcact caaatcctgt
    21061 ccatcggagt tttcacatat ccatcgacac ggcttcttct ttttatcaac tttccccact
    21121 acaatccatt tttaccatcc ctaaaggaac atactttgct aggctacgac gggtaattta
    21181 tttcatacct gcaacaaaca aagagaccgc ccctggcgca ccccgggagc agtctcttct
    21241 acatattaga tttaaggtgt ttcagtattt tattctcctt taatagcttt gatacctggc
    21301 aaaactttgc cttctatggc ttcgagtaaa gcacctccgc cggtagaaac ataactaact
    21361 cccttagcca ggccgaattt attgacacag gctacggaat cgcctcctcc caccaacgag
    21421 aaagcgcctt ttttggttgc ttctacaacg gcttcaccga cggatttgga tccatgagtg
    21481 aaattttcga attcaaaaac gccggcgggg ccattccaca atatggtctt ggaattttca
    21541 atgacttcgg cgaaaagggc ctcggttttg gggcctatat ccatgccttc ccatccatcg
    21601 ggtatggcgt caatcgatac aaactgagtg ttggcctcat tactgaaagc atcgccaacc
    21661 ttggaatcaa tcggcaagac cagtttaacg cctttttgct tggctttttc aatcaattcc
    21721 agagccaaat caagtttatc gttttcgcaa atagaattgc cgatactgcc ccccatggcc
    21781 ttgacaaacg tgaaagccat accgcctgta atgataaggt tatctacttt atccagcaga
    21841 ttttcgatga tatctatttt ggaagaaacc ttggctcccc ccatgatggc cgtaaaagga
    21901 cgtacaatat cgcgcattac tttttcaacg gcctgcactt cactgttcat cagataacca
    21961 aacattttgt tgttatcatc gaaataatca gcaataagag ctgtagaagc atgagcacgg
    22021 tgagctgtac cgaaagcgtc gtttacatag caatcggcgt aagaagccaa ttttttggta
    22081 aaagctttct ggctttcctt gactattttt ttagcagctg ctttttcttc gtcgctggcg
    22141 tcttcggcca aatcacgggg tttaccctct tcctcggcgt aaaaacgcag gttctccaac
    22201 agcagggctt cgccgggctt caaagcagcc acctgagcgt cggccttttg acaatcttcg
    22261 gcaaagtgta cgggtgcacc cagacaatcg gcaacgtgtt gctgaatggc ctttaacgaa
    22321 tacttaggat ccggattctt tttgggacgt cccatatgag aacctatgat cggcattccg
    22381 ccttccttaa tgattttctt caatgtaggc atggcagcgc gaatacgcgt gtcatcggtg
    22441 atgtttttat tttcatccag agggacgttg aaatccaccc tgacaaaaac ctttttccct
    22501 gaaaaattaa attgctctat cgattgcata aaagtatgaa gtaagacgtc gcgaagatag
    22561 agaaatattc tgttatatgc tattttctcc ggattttttt attccaggtg gaaaacttct
    22621 accaaaggta tagtgatcgg agaattgtcg ggattggttt ccataatatc tgccatataa
    22681 tcccaccatt ttttttgtat ttcattgaaa cctagatcct gagaagacag atttccttct
    22741 actttttgaa caccaaacag aatattggtt tcttcatcca aaaaaatgga ataatcgctg
    22801 actccgcttt ttttgatcag ttcggctatt tcgggccaaa tttgatcgtg gcgtttgatg
    22861 tactcctctt tcatgccggg agtcattttc attttaaagg ctactctttt catgatttta
    22921 ctatttgcga gtgttaaaat aatcggtact ttctcattta aggtatttat ccagccaggc
    22981 aaaaaatctt ctttgccata atatggcgtt ttgaggcttg gaaatccagt gattttcgtc
    23041 cgggaaaacc aacagttcgg caggtatacc ccgaagtatt gcagcattaa aagcggccat
    23101 tccctgcgaa gccaggatac ggtaatcttt ttcgccgtga atgcaaagaa tgggtgtatc
    23161 ccacttatcc acaaaacgat gaggagagtt ggcgtagctg agctgggctg ttttattatc
    23221 ccgttcccag taaggaccac ccaaatccca atcggcaaac cacatctctt cggtttccag
    23281 gtactgttgt tccagattga aaatgccgct gtgcgaaata aaagccttga aacgcttttc
    23341 gtgattgccg gccagccaat agacggaaaa accgccgtac gaagctccca cgcagcccaa
    23401 acgatcacgg tctacaaaag gttctgcact cagcgcgtca atggccgaaa ggtagtcttt
    23461 catattctga ccgccgtaat ctccgctgat ttgttccaac cattcctgcc caaagcccgg
    23521 aagtccgcgg cggttaggag ccacaacgat gtaaccgttt gcagccatca gttgtagatt
    23581 ccagcggaaa gaccagaatt gactgaccgg actctgaggt ccaccctggc aatataacag
    23641 ggcgggatat ttcttagagg agtcaaaatg aggaggatac actacccaga ccagcatttc
    23701 tttattgtcg gtggttttaa cccaacgctc ttccactctg cctaaactga gctgatcata
    23761 aatctctttg ttttcgcggc tcaaatcgag catctccccg ctttggggat cgatccggat
    23821 aatttcgttg gcctgcgata gagaatgtct taaaccgacc agatactcgc ctgccacatg
    23881 tacatcggca aaatcgtagt cgccaccggt aatttgcctg atgggacttc catcggtaaa
    23941 tgcctgataa atatgcactc tgccgtgcca acagctcaca aaatagagcg atttggcatc
    24001 gtcagcccat accacagaat tagcttcctg atcgaaatcg gcagtgaggt ccttcatttt
    24061 accggtgctg agctcataaa ccatcagacg gtttttatcg gactcatagc catcgcgcga
    24121 catactgcaa aaggccagac tgcctccgca aggtgaaaat acgggattgg tatcgtagcc
    24181 gggcaattcg gcggtaatgt tttcagtacg aaagttttct tccaaaaagc agaaataaat
    24241 atccgaatcg gtcgaacgtg aatattcgac acctgttttt ttgcgacagg tataagccac
    24301 tattttcccg tcgggcgacc aggccagatg ctctatgcct ccccaaggct tcattggaga
    24361 ctcaaagggc tcatcttgaa gtaaatcacg cacattgctt aaacctttgg aggagaaatc
    24421 ggctacaaag ggatgaggaa tagtttctac ccattcatcc caatgcttat acatcagatc
    24481 atcaataatc cggccagtgg tcttatctaa atcgggataa taatctaccg cccttttgcc
    24541 gtattttacc tgggccacgt agagcagttt tttcccatcg ggcgaaaaac gaaaggcatc
    24601 tatgcctccc tcgaccgagg tcagtttttt aggttttctg ccgtcgggca gcatctgcca
    24661 taattgagaa ctgcccgata ctgtagaaag ataatagatt ctcttgccgt cggccgacca
    24721 ttgggctgcc gattcagaaa ctgccgtagc ggtaagctgc cttctatgtt ttccctccat
    24781 gtcgaccaaa tacagttcgc ggtttccctt gttttgagta atactgtaat agctcactcc
    24841 gtacaaaaca gtctttttat cgggggaaag ctggatatcg ctcaccctgc ccatactcca
    24901 taagatttcg ggagtcatta gatcgccggt caattcaagt tgaggtttgc ttataggaat
    24961 ttctttggac ctgtcaccct cttttgggga gcaggaaaaa ctaagtgccg caatagccat
    25021 taatatcggt attttgttgt tattcataac taataatgta aatacaaagt taatcactag
    25081 caaagatatg ccgcttttct aaaaaaaaca aaaggtgccg gggtataccg acaccttcaa
    25141 tactttaagt ttgtttggtt ttatccgagg taagtcttaa ggaatttgct cctggaagtg
    25201 ctacgtaaac gccgtatggc tttttccttg atttgccgga cacgctcccg ggtcaaaccg
    25261 aatttatcgc cgatttcttc cagcgtcatt tcctgacatc ctaaaccaaa aaacatttta
    25321 acaatttcgc tctcgcgttc tgtcaaagtc gacaaggcgc gatcaatttc caggctgagc
    25381 gactcgttca taagcgaacg gtctgccata ggggaatcgt cattgatcaa cacatccaac
    25441 aggttgttgt cttctccttc cacaaaggga gcatccaccg aaatatgacg gcccgacacc
    25501 ttgagggtat cgttaatctt atctacgggg atattcaact cttcagacaa ctcttcgggc
    25561 gaaggtttcc gttcgttttc ctgttcgaat ttcgaaaatg ctttggtgat cttgttcaaa
    25621 gaacccacct ggtttaatgg aagacgtact atacgggact gttcggccag agcttgcaaa
    25681 atagattgtc tgatccacca cacggcgtag gaaataaact taaagcctct ggtttcgtcg
    25741 aatttttctg cagccttaat caagcccaga ttaccttcat taattaagtc gggtaaactc
    25801 aggccctgat tttgatattg tttggccaca gaaaccacga aacgtaaatt agcacgggta
    25861 agtttttcca aagctgcgcg gtcaccgttg cgaatgcgtt gagccagttc tacttcctcg
    25921 tccaccttga taagttcttc ccgtccgatt tcctgaaggt atttgtccag agaagcgctc
    25981 tcgcgattag tgatggattt ggtaatttta agctgtctca tgctttatat aaaaaatagg
    26041 agtgcaaaga tacgttttat ttttgaatta ttcatgttat ttcccaaatt taattcgcaa
    26101 cagactttac tgagtcaaat ctattgcgta ataggctact ttcccgctgg gataaatccc
    26161 cacaataaaa agcgcagcct cgccgaaacc ggcatttttc tgaacatcac gtacgatctt
    26221 ctcaaaatca gcgacggatt caatgcgctg gtcgttcact tttaaaatga taaatccttt
    26281 gcgcacgccg gcttctttca attttccttc ggtcagtccg cctacccgga tgccatagct
    26341 aatgttcaat tgcctttttt cgttggcgct gagttcgtta aaagcagccc ccaagacttc
    26401 tatgccggcc tctttgacaa tggcggtact accttgtaaa ttgcgtaaaa ccacattcat
    26461 attcaactcc tttttgtctc tgagtacggt aagtcttacc ttatcgccgg gacggtaacg
    26521 actgattttt tcctgtaact cagctgctct ggttactttt tcgccatcaa cggcaataac
    26581 aatatcgcct tctttgacgc cggcatccaa tgcaccgctg cgttcaccaa cagcatgaac
    26641 atacacccct tcggtgacgt ctattttctt ctctttagaa agttccggag taatatccga
    26701 aatatcgact cccaatatgg ctctttgtac ggaaccgtac tccttgatgt cggtaactac
    26761 ctttttaaca atgctcacgg gcacagcaaa ggaatagccc gaaaaagcat tggaactcga
    26821 tatgatcgcc gtattgattc cgaccagttc tcctctggta ttgaccaaag cgcctccact
    26881 attgccggga ttcaccgcag cgtcggtctg aataaacgat tcaatgctga gattggaata
    26941 actcttatcc cgctgtactc tattttgatt attgcctctc aaaattccta tgttcctgcc
    27001 cttggcactg acaatacccg cagtaaccgt agaagtcaga ttcagaggat taccgacggc
    27061 cagcacccac tcgccaacct tgagttcatc ggaatttccg aaggtaataa aactgaggcc
    27121 cttttcttca attttaatca gagcaatatc ggtactgggg tcggttccaa tcactttggc
    27181 ctgataatta cgcctgtcgt tgagagtgac cgatatttcg tcggctcctt caataacatg
    27241 attattggta acaatataac cgtcagccga aatgatgact cccgaaccat aaccggtttg
    27301 gggcttcgga gtctggttgt aatcccggta accgaaaaaa tattcaaaaa tatccaaggg
    27361 accgctgctg ccaaccgacc tcatcgtttt agaagcaatg tgaacgacag catgtacggt
    27421 ttggttagca gcacgagtaa agtccgtttc tatggcaggc actacttcag ccttaaccaa
    27481 acgcaagggt tgttccactt gattgatact ttgttgttcg atcggtctta tcaaacggtc
    27541 agataatttg gaaaccgtta aatacgagac tccggcgctc agcagcacca gcgacaagac
    27601 gattaaaaaa tttctccaaa actttttcat tgcagtataa tttaatattc tagtttacta
    27661 ttaaggtaaa tctctgcata taactacaaa aacaattcct aaacggatga aataagtttt
    27721 tatttcatcc caattaacag tgtcttgctg taattaataa tgattacctg cctctttaac
    27781 aagattttag aattaatgaa ttcctgttaa aaaaatgaat gatgacaact gtatcagtac
    27841 agacattttg tcagtccaaa atggttagaa agtaaaaaag gggacaaaat ggtagaaata
    27901 tcaaattatg cactattttt gcatccaagc agagaaccgg cttgtcgctt ttttaaaaga
    27961 ggaaagtccg ggcaacgcag agcgccatac tttctaacgg aaagctattt
//
LOCUS       DAOSTD010000019        27942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1886_length_27942_cov_14.858716, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000019 DAOSTD010000000
VERSION     DAOSTD010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27942
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1886_length_27942_cov_14.858716"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..653
                     /locus_tag="PLI37_02255"
     CDS             90..653
                     /locus_tag="PLI37_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOQ57874.1"
                     /translation="MKKLAFLLVFSLLLVGAYGQSATNEVINTILNGYSARAYTSEPV
                     SDKDIELILDCGVKAPSARNAQPWKFTVIKNGDLVGQIIRNATPGNVVILISGPESAE
                     QGMSVNIDCALATESMFIAALGLGLGARIYTAPIAGVNARKQDFQIPEGYRAVTVLRI
                     GHMDKNVDAVTAASPRKAKAELVNVVE"
     gene            664..1932
                     /gene="purD"
                     /locus_tag="PLI37_02260"
     CDS             664..1932
                     /gene="purD"
                     /locus_tag="PLI37_02260"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOQ57875.1"
                     /translation="MKLLLLGSGGREHALAWKLVQSPKVERLFIAPGNAGTSEVGVNV
                     PLAVDDFAAIRDFALKEQIDMLVVGPEIPLVKGISDFFASDPLTAGIPVIGPSKSGAR
                     LEGSKDFSKAFMKRHNIPTAGYLSVTAENVQEGLDFMKKLRPPYVLKADGLAAGKGVL
                     IIQDLEEAQKELKTMLDGKFGEASKTVLIEEFLSGIECSVFVLTDGRQYKILPEAKDY
                     KRIGEGDTGLNTGGMGSVSPVPFADKLFMQKVEERIIRPTIEGLRKENIDYKGFIFFG
                     LIKVDEEPYVIEYNVRMGDPEAETVVLRIKSDLVDLLEGVARGNLDEKKIEFDPRTAV
                     AVMLVSGGYPGDFEKGKEITGLDRVKESIVFHAGTKIQDGKILSSGGRVLAVSSYGNN
                     IAEALSLSFENARKIHFENSYFRSDIGFDL"
     gene            1966..2973
                     /locus_tag="PLI37_02265"
     CDS             1966..2973
                     /locus_tag="PLI37_02265"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HOQ57876.1"
                     /translation="MSRRNYIRHLSRTNLLWVVGLLLCVLGVVFSTMLLTPGTEVLYS
                     DEVISPFWKPVRGFIPGEWLPVLIQFLLLAANAILFQFIVRRYELLRTLGVLTFFIYC
                     FITAASIPLHCLIPGSFAALFIGLAYFSIFESYRKENCNNQVFNASFLIVLAALFVPP
                     LIFLLPLFWLAFNIVNTMNFKRWLVSLMGALVVFWLLGGVLFLSDRLELIPAFFEACI
                     QYGWVFSAELPTMGIVFFSMLFLLVMLAIVNYLRQVYSEKIGVRSAFSFLLISLLVFV
                     VSMVIFPAYKVDFFLLSIGPASVMLSHYYGSEVNLFGRLALYVFLLTAVGNYLSFFFS
                     F"
     gene            complement(3030..3755)
                     /gene="mnmD"
                     /locus_tag="PLI37_02270"
     CDS             complement(3030..3755)
                     /gene="mnmD"
                     /locus_tag="PLI37_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOQ57877.1"
                     /translation="MKQQNEFIPAKSCEICPTADGSQTLYSNRVQEHYHSTFGALQES
                     RHIFIQAGLLEAYRRKTAGTNPSLKVFELGLGTGLNALLTILEAGKLQLNLSYDCIEA
                     YPLSPDLIKALNYPALFSEQDQNKAAAVLDFIHAAEWNVRHTEPASHTTFTKFRGDFA
                     LFELPQSYDLVYWDPFSPEKQAGMWTEELFASMYKHMTTGGILVSYCAKGEIRRRLQR
                     AGFGVERLQGPPGKREIIRATKE"
     gene            complement(3758..4537)
                     /locus_tag="PLI37_02275"
     CDS             complement(3758..4537)
                     /locus_tag="PLI37_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ57878.1"
                     /translation="MSTMLFQDIIFGPVKSRRLGISLGINLLPLSKKFCSFDCLYCEC
                     GLGDMKNRGILPKTEQVIAALDLKLQELLAKGVVPDVLTFAGNGEPTLHPDFPAIVDA
                     VVVLRDRMVPQAKVSVLSNSSRIDKAEIRAALLKVDNNILKLDSALESTVQWLNRPVK
                     KAYSIQQQIEYMQLFEGKLIVQTLFLRGTFLGKIIDNTTEEEITAWIEALRKIKPLSV
                     MIYTLDRETPVQSLEKIPLKELETIARRLRDATGIPSTVAG"
     gene            complement(4544..6142)
                     /gene="nadB"
                     /locus_tag="PLI37_02280"
     CDS             complement(4544..6142)
                     /gene="nadB"
                     /locus_tag="PLI37_02280"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOQ57879.1"
                     /translation="MVRKFDFLVIGSGLAGMSFALKVADKGTVAICCKSALEEANTFY
                     AQGGIASVTNPATDNFQKHIKDTLICGDGLCDKSVVEKVVKEAPAQIAELIHWGVAFD
                     KDQSGHYDLHREGGHSENRILHHQDATGAEIQRSLIRRIQQHPNIQVFDHHFAIEILT
                     QHHLGATITRQSEHIECYGAYVLNRRTRAIHTFLAKVTMMATGGIGNIYQTTTNPVIA
                     TGDGIAMVYRAKGQVKDMEFVQFHPTALYHPGERPSFLITEAMRGYGAVLRTRDGEEF
                     MQKYDDRASLAPRDIVARAIDTEMKIRGTDYVYLDVTHKEPAETKKHFPNIYKKCLSI
                     GVDITKEYIPVAPAAHYLCGGIEVDKNAESSIDRLYASGECARTGLHGANRLASNSLI
                     EAIVYADSAAKHALGRLKDIGFEERIPLWNDEGTRLPEEMILITQSAKEVGQIMSSYV
                     GIVRSNLRLKRALDRLEILYQETERLFIRSVVSKEICELRNIINTGYLVIKQAQLRKE
                     SRGLHYTIDYPNKNELGHTYPLNS"
     gene            6269..9028
                     /gene="leuS"
                     /locus_tag="PLI37_02285"
     CDS             6269..9028
                     /gene="leuS"
                     /locus_tag="PLI37_02285"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOQ57880.1"
                     /translation="MEYNFSAIEKKWQEEWIKRQTYKVYEDRSKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIVARYKRLQGYNVLHPMGYDAYGLPAEQYAIQTGQHPAITTAN
                     NIERYREQLDKIGFSFDWSREVRTCEPKYYKWTQWAFIQMFKSYYNTELDRARPIEEL
                     IELFESHGNKDIPAYGGEELPEFSAAQWKSFDNLKQQELLMHYRIAYLGDSMVNWCPA
                     LGTVLANDEVSEGLSLRGGYPVEQKLMRQWCLRISAYAERLLQGLEKIDWTDSLKETQ
                     RNWIGRSEGAEMNFGVKDSDLTIPIFTTRADTIFGVTFMVLAPESELVASLTKPEYKA
                     GVDAYLQKVKKRTERERIADRRVSGVFTGSYAINPLSRAEIPIWISDYVLAGYGTGAI
                     MAVPGHDSRDYAFAKHFDLPIIPLIEGCDISEESFDAKEGIMKNSGFLNGLSVPEAIE
                     KTKAYLIKNQLGRIKVNYRLRDAIFSRQRYWGEPFPVYYKDGLPQVLDESSLPLELPE
                     VDNFLPTEKGEPPLGHAKGWKTEEGYPLELNTMPGFAGSSAYYLRYMDPHNEEALVSV
                     EANTYWRQVDLYVGGTEHATGHLLYSRFWNKFLYDLGVVCEDEPFKKLVNQGMIQGRS
                     NFVYRIKDSNTFVSNNLKDKYEYTAIHVDINIVNNDVLDLERFRNWSPDYEDAEFILE
                     DGKYICGWAIEKMSKSMYNVVNPDQIVEKYGADTLRLYEMFLGPLEQSKPWDTNGIDG
                     VHRFLKKLWNLCVSEDEFSFSDAAPDKQELKSLHKLIKKLNWDIEHFSFNTAVSAFMI
                     CVNELSAMKCNKRDIIKPLIILLAPFAPHFSEEIWQALGEQTSVCDAVWPELNEDYLV
                     EDQVTYAVSFNGKTRFMLDVSSEAGKEEVEQLALAHENSQKWLEGKTPKKIIVVPGKI
                     VNIVV"
     gene            complement(9199..10209)
                     /locus_tag="PLI37_02290"
     CDS             complement(9199..10209)
                     /locus_tag="PLI37_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HOQ57881.1"
                     /translation="MKNALFVVSITFLLSFTLRLSAQDNRFNPEGSSTPSVHDPVVAK
                     QGNTYYLFCTGFGISVLSSTDMINWKREKPVFSEPPAWAVEAVPGYRGHTWAPDIIKH
                     QGKYYLYYSVSTFGKNNSCIGVATNKTLDPGSPDFKWEDQGMVLRSIPNRDMWNAIDP
                     NIIIDEDGTGWMSFGSFWEGIKLVKLDSSLCALAEPQEWYPLSRRPRLINDQDVEPGG
                     GAVEAPFIFRKGSFYYLFVSFDMCCRGAQSTYNVVVGRSRKVTGPYVDREGNVMTKGA
                     GTLVVKGNERFPGVGHNAVASFGGKDYLFFHGYDMERNGRSILLIREIKWDDQGWPSV
                     VL"
     gene            10519..11808
                     /gene="metK"
                     /locus_tag="PLI37_02295"
     CDS             10519..11808
                     /gene="metK"
                     /locus_tag="PLI37_02295"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOQ57882.1"
                     /translation="MGYLFTSESVSEGHPDKVADQISDALVDAFLAYDENSKTACETL
                     VTTGQVVLAGEVKSSIYVDVQEVARQVIKKIGYTKSEYRFDGDSCGVFSSIHEQSEDI
                     NRGVDGAAKIDPMNQGAGDQGMMFGYACKDTDNYMPLPLELSHRLLRKLADIRREGKV
                     MTYLRPDSKSQVTIEYNDKGKPERIHTIVVSTQHDEFIPAGKGLSQEQADAEMLSVIR
                     KDIQQYLLPAVFNADPGLKALLDDKLILHVNPTGKFVIGGPHGDSGLTGRKIIVDTYG
                     GKGAHGGGAFSGKDPSKVDRSAAYAARHIAKNLVAAGVADEILIQVSYAIGVAHPVNI
                     FVDTYGKSRVKISDGEIAEKVVQSFDMRPKAIEQRLKLRKPIYSETASYGHFGREPQK
                     LVKHFSSRYEGDKKIEVELFTWEKLDYVEQIKKAFGL"
     gene            complement(11803..12417)
                     /locus_tag="PLI37_02300"
     CDS             complement(11803..12417)
                     /locus_tag="PLI37_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOQ57883.1"
                     /translation="MRYLLFTLFLLLSINSRAQIDEGINRLLPARPPQSIPARIEGGD
                     TVLLVNLNPVYVYPGMVFKNKREERYYSRLVRDVKLCLPLARIVSGTLIETAEYLETL
                     PDDKAREKHLRQMQRDLIKDYEPTLRKMSYSQGKILLKLIVRECDSSPYEIIAAYRGY
                     FAANFWQGVAKLFTADLKTDYNPTDNQTDRLIERIIIMVEQGML"
     assembly_gap    12532..12631
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12724..13611
                     /locus_tag="PLI37_02305"
     CDS             12724..13611
                     /locus_tag="PLI37_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57884.1"
                     /translation="MNVLRFFKRRSAAPDPSVVRGKNNILWYCALIIKVLHSSPGLYA
                     QTISAREWYGDAKSMSMGDAMLISQWLFSEPSHQRQQDNMLGVQRAQATYYLPFNRAS
                     LAVTGLMIQLRSPLADLQLNMGRSGGDDYNESLISLGCARSPTENLSGGFRLGIYSLA
                     GNQLHEGTALLTECFVYYLVNTQWRLGAYMFNPIAAKVSGIPLIQSYHLACAYRPDSR
                     LDFVLEMEKPQRQDILVHLGMEAAVLKCLRCRLGLIYPVMQPSFGLACSFKKFSLEAA
                     CRLHKQLPASYAVTLHYTP"
     gene            13583..15664
                     /locus_tag="PLI37_02310"
     CDS             13583..15664
                     /locus_tag="PLI37_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOQ57885.1"
                     /translation="MLSRCIIRLDCLLLLILISSKLLSAQESYDTDDLEHILSDWVEQ
                     NPEADAEALAQELYELLEQPVNLNDAGREQLESLPFLSPDQKEKLAYFLYRRKPLYSL
                     SELLLCEGMDEVSFRRIRPFLYIGAADQTSEKLDFKKLLSCGKHEIRAYTAAGFQPRQ
                     GLLNAGDSASKAEGKAYLGLPFSGVCKYRYSYGRQLQYGLVMEHDLGERPVDFLSFHI
                     AVHQLAKINTLILGDYRLKLGRGLLVSSGFSSGKTTSPSALASVPKMLSSHFSCSESA
                     YFRGAAIDLSLSEHLQLLAFASGRKLDAVLEEGSFTSVRSDGLHRTSKELSYRNALRQ
                     YSTGAHIGINQSYFQAGANVLYYRFNAFCNPDLKPYSLFYFRGRDNLNFSLDYRFRAG
                     TALFSGECAFDNKCHTAQFHQLQYRPHPLLNMVHSFRYYSPAYQAYFAAAFSENSRVC
                     NETGWFTAMEYRLFERLKMDAYLDVFVFPWLKYGVNAPSSGHDLGLELSSCIVNGLEW
                     SLRYREKTKTANLPVEGRKGITPCADNTKRQLRLQLKAGFGGLSLKTKAELNRFNVAG
                     FETSTGALFSQECRYKTPDERLSLSLQYAMFDTEKYENRIYFYEYDLPGVFSIPAYSG
                     TGSAYVLCCKLKPGRNFQCWARLRHRAYSDREEVGTMLDIVRGPCSTEFRLGFTYRFS
                     AKSGLSGRNQP"
     gene            15789..>16228
                     /locus_tag="PLI37_02315"
     CDS             15789..>16228
                     /locus_tag="PLI37_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOQ57886.1"
                     /translation="MKNFKLLNNVFGWFVFLVAAVTYLLTIEPTTSFWDCGEFISAAY
                     KLDVGHPPGAPFFMLTGRFFANFASGPEQVAKMINAMSALFSAFTILFLFWTITHLAR
                     KVVVKDNDNMKPAEMITVLGAGLVGALAYTFSDTFWFSAVEGEV"
     assembly_gap    16229..16328
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16329..19246
                     /locus_tag="PLI37_02320"
     CDS             <16329..19246
                     /locus_tag="PLI37_02320"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOQ57887.1"
                     /translation="AYTFSDTFWFSAVEGEVYAYSSFFTAVVFWSILKWEEEADKPHS
                     LRWIIFISYLMGLSIGVHLLNLLCIPALVLVVYFRKNQNANAKGAALALLGSFVLIAI
                     VLYGVVPGFVNMAGWADLLFVNVLGFSFNSGAVAYILVMIAVLCWGLAESYRGKNEFR
                     MKLAFALNIVLSGIPFFGNSPWVWILLCGLTLAVVFRWKKINGKLINTSLLALMVMLI
                     GYFSYGVIVIRSNAQPPMDQNSPNNMFTLERYLNREQYGETPLIYGQTFASDIKWKRQ
                     GNICVPQYKKRGPVWSKQVKTSPDEKDRYFISGYNERPVMADELNMFFPRMHSNKPEH
                     VVMYKDWVNFKGTPVKYNKCGQIETVYKPTMAENLRFFFSYQINFMYWRYFMWNFSGR
                     QNDIQNLGGEANNGNWITGFNFIDKHLVGDQSNLPPELADNKGRNRYYMLPLLLGILG
                     LLYQAYAGKKGVQGFWITLLLFLMTGLAIVLYLNQPPLQPRERDYAYAGSFYAFCIWI
                     GMGVPALVSILRKALKPVPSALLAGVLGLGIPVLMASENWDDHDRSDRYTARDFAYNY
                     LIGCDENSIIYTNGDNDTFPLWYLQEVEGIRTDVRVCNLSYLQTDWYYDQMLRPYYES
                     DAFPVSWDKSKYSNGKRDIARVIPRTQDTISLPFAMNWLGSDDASTKRVQGYGDNVDH
                     IPANKLYLDVDAEKVLENGYLEPKYAEHIVPRMFIDLSAKNYLGKHEIMTLKLLEENN
                     WERSMYYAVTVDPALMTMLQRYFLHEGLIYKLAPLESNRAINTDKMYDNMVNKYRWGG
                     IKENPDIYLDENNLRMCRTHRQHFGFLIKALYQEGKYDMALQAIDKCLEELPTEHVPV
                     NFIDGGMAGMLEITEVLYDLGEKERSLAIANEGMDLCIQNLNWFFSLNDPLLRASGRS
                     VNNQLYVMQELRNFLQRAATEASALENPSKVDVAFMEAFNKNTQHFGQFYQQYQRLR"
     gene            19316..19858
                     /locus_tag="PLI37_02325"
     CDS             19316..19858
                     /locus_tag="PLI37_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ57888.1"
                     /translation="MSQKTIYLSFDDGPIPGVTPWVLDTLDRYGIKATFFCVGENVAR
                     YPELFEQIKTRGHQTGNHSYNHLRAFRTNPDDYLANVEKADKLIGSSLFRPPHGELTY
                     AQFRALRKKYELVQWDLISRDYNAKLSPDEVFDIVRKGVRKGSILVFHDSLKAEKNLR
                     AALPRSIEFCLAQGYVFDVL"
     gene            complement(19837..21348)
                     /locus_tag="PLI37_02330"
     CDS             complement(19837..21348)
                     /locus_tag="PLI37_02330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ57889.1"
                     /translation="MEKPLISVIVPVYNMEAYLAECIDSILKSEYSPFELIIMDDGST
                     DKSLQIALEYAAKHPNIKAETQANAGVSAARNAAIELAQGKYILPVDADDLIHKDFLE
                     KAVEVLQKQPSVKVVTARAEFFGDKTGKWLLPDYSPALLARKNMLSACAMYRKSDWTA
                     CGGYCNYIPGREDWDFWISMLKNGGEVVKLNFTGFYYRYRSNSKRISDRGKKKQIIDI
                     LNQRHADFFKRELKGKLHYNRTFSKFFNSINNLLERTKLVLHEDYKNLHPFLSSLPEQ
                     FDTSGDLMFQARNTIKSYRVEGMDVVVKSYGNPLFINRIIYGNIRASKAKRAYNYALK
                     LRDYGIGTPQPLAYIEIKKGLILHKSFFISLKSTFPNRLSDLCTETPAKDVDKLVRGI
                     AVFTAKLHDLGILHKDYSSGNLLYEINKDEVLLQVIDLNRMRFGRVGRKKGCKNFERL
                     CLNDEILSRIAEEYAEVRSFDKTICVEEVLKYNRKHRNRRSKKQVQRQSTSKT"
     gene            complement(21375..22094)
                     /locus_tag="PLI37_02335"
     CDS             complement(21375..22094)
                     /locus_tag="PLI37_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ57890.1"
                     /translation="MPKPLHIITPVKDSYETAAGSIRAIKESDLPPSCSYTIYNDFST
                     ASNKKLLEELTLELGFDLVHLEDLTTNPSPNYRLVLQLAQKKALEAGADLMIIESDVY
                     IKQDTIKKMQAACAELDNPGMVAAVTVDESGVVNFPYLYASSYPSSIIKTRKRLSFCC
                     SILSNSLLSAYDFKFLDPDKSWFDVFISHKSRKLGFNNYLITKLPVLHRPHSSRPWKQ
                     LKYSRPWKYYFLKILKQRDKI"
     gene            complement(22096..22491)
                     /locus_tag="PLI37_02340"
     CDS             complement(22096..22491)
                     /locus_tag="PLI37_02340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOQ57891.1"
                     /translation="MASIFSKIVAGEIPSYKIAEDEHFFAFLDIHPLMPGHTLVIPKK
                     ETDYLFDIDDRELAAMIVFAKKIALALKTAIPCKRIGMAVVGMEVPHAHIHLIPINKE
                     TDMLFSKPKLNPTAEEMKAIAEKISKSLK"
     gene            complement(22499..22975)
                     /gene="greA"
                     /locus_tag="PLI37_02345"
     CDS             complement(22499..22975)
                     /gene="greA"
                     /locus_tag="PLI37_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOQ57892.1"
                     /translation="MATNYMTENGLNKLKEELEHLETVERAYISKQIAEARDKGDLSE
                     NAEYEAAKDAQGMLEMKIANIKELIRNARILDESKISTDTVQILTNVHLLNLKTNQEI
                     QYTIVPDNEANLREKKISVSSPVAQGLFGKKEGEVVDIEVPAGIITYRIKKIEHAK"
     gene            complement(23155..>23505)
                     /locus_tag="PLI37_02350"
     CDS             complement(23155..>23505)
                     /locus_tag="PLI37_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOQ57893.1"
                     /translation="PLSSLEGKVILLDFTAFQANYSAARTIEMRELYNRYELYGFEIY
                     QVSLDPDEHFWKTGALNLPWICVRDKNLLNSAYLSMYNVSNLPSYFLIDRQGNLAGRD
                     ETIQNLDEAIQKLL"
     assembly_gap    23506..23605
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23606..24222)
                     /locus_tag="PLI37_02355"
     CDS             complement(<23606..24222)
                     /locus_tag="PLI37_02355"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HOQ57894.1"
                     /translation="MRNLFFITGLFILLQACSDNTSFVVEGHVENAENKTLYFEFFGL
                     SNTSIIDSVVLDESGKFKFKAPLPEYPDFYRLRLDRQRLFLVVDSQVHINIKASAEAF
                     STGYRVSGSDYCVQMQELALLQSNTRRQYDSIQALFNNKEIEEKAYIDLVLNCIDEHK
                     KQAQGYIFSNPSSPVAYFALFQRIHKLLIFDPFSEKDNRHFAAVAT"
     gene            complement(24290..24985)
                     /locus_tag="PLI37_02360"
     CDS             complement(24290..24985)
                     /locus_tag="PLI37_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOQ57895.1"
                     /translation="MKKLVVLTGAGMSAESGIKTFRGSGGLWENYPIEAVATPEGWKA
                     NPELVTQFYNDRRKQLLEVEPNAGHYELAAMEKSFDVQIITQNVDNLHERAGSSNVLH
                     LHGELTKVRGEVHSEYIVELKPEEYEVKIGDCTPYGDQLRPHIVWFHEMVTMIDPAMD
                     IVRQADYFAVIGSSLVVYPAAGLLHLVPRRSPSYVIDPEELSLCRFPDIVHIKLPASK
                     GVKKLHSLLLEQK"
     gene            25144..>25543
                     /locus_tag="PLI37_02365"
     CDS             25144..>25543
                     /locus_tag="PLI37_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOQ57896.1"
                     /translation="MERISSLQNPRIKQLLRLQAKARERREQALVLVEGAREIQLALS
                     KGYCPEQLFICPELYAHSDYPGLVGEIADEIKTELSLPVFEKLAYRKGSDGLLATLRP
                     KSHLLEELVLSAHPFIVVLEAVEKPGNLGAI"
     assembly_gap    25544..25643
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25644..26096
                     /locus_tag="PLI37_02370"
     CDS             <25644..26096
                     /locus_tag="PLI37_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOQ57897.1"
                     /translation="PFIVVLEAVEKPGNLGAILRTADAAAVDAVIVCDPLCDLYNPNV
                     VRSSVGCLFTVQTAVCSSQQALDWMRRHKINILAAELQASQWYHQTDMRLSTAIVMGT
                     EADGLSPFWLNNADERIKIPMRGYIDSLNVSVSTAVLCFEAMRQRGFR"
     gene            complement(26078..26908)
                     /gene="murI"
                     /locus_tag="PLI37_02375"
     CDS             complement(26078..26908)
                     /gene="murI"
                     /locus_tag="PLI37_02375"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOQ57898.1"
                     /translation="MNAKPIGVFDSGYGGLTILEEFRRKLPQYDYLYLGDNARTPYGS
                     RSFELVNQFTLEAVKYLFGQGCRLVILACNTASAKALRNIQQQYLPSLEYNCRVLGVL
                     RPTVEALGSLTKSGHVGLVGTQGTIQSGSYPIEIQKLFPDIHLESEACPMWVPLVENG
                     EHESPGASYFVKQHLDRLFQRDPLIDTLILGCTHYPLLINQIRKHSPPGLQIVVQGKL
                     VAESLSDYLQRHPEIEELCSKGGSCRYLTTEAEEKFEEMASVFSHTSIRVEQVHLNPL
                     "
     gene            complement(26923..27429)
                     /locus_tag="PLI37_02380"
     CDS             complement(26923..27429)
                     /locus_tag="PLI37_02380"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOQ57899.1"
                     /translation="MYKHFIVAVLCFLPLLAVAQEFGYVNRAEIFQAMPETTEAMKKL
                     DDLAKNYEAELLIMQEEYQKKGSEFVAQRDSLPESIRIRRMTDIQDLEQRIQGFYQES
                     QEHIQRQQQELIIPINDKLTKAVQAVGEELNMVYIFDTSANMGLMYWSPSKCKDVTPL
                     VRAKLGLK"
     gene            complement(27493..>27942)
                     /locus_tag="PLI37_02385"
     CDS             complement(27493..>27942)
                     /locus_tag="PLI37_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOQ57900.1"
                     /translation="QKYALVDMEYIFSRIPAYEMANDQLNQVSKKWQAEVDALANAAK
                     ELYTKYQAEMVYLSDDMKKNKENEILAKEKELADLKRKYFGSEGELYKKRESLLTPIQ
                     DEIYNAVKELAEEQSIMLILDRASGVNIVFASPAIDISDQVLKKMGY"
BASE COUNT         6829 a         6075 c         6855 g         7783 t
ORIGIN      
        1 ggaagggtaa aatgaacttt ccggacggac tactgaaatt agttggaaga aatgaatatc
       61 tttgagctgt atttttaaac agtaacatta tgaaaaaatt ggccttcctt ttagttttta
      121 gcttgttgct cgttggcgcg tatggacaga gtgcaaccaa tgaagtgatt aataccatac
      181 tgaatggcta ttcggccagg gcatatacct cggaaccggt ttcggataag gatatcgaat
      241 tgatactcga ttgcggggta aaagctccca gcgccagaaa cgcccagccc tggaaattta
      301 ccgtcatcaa aaacggggat ctggttgggc aaatcattcg caatgccact ccgggcaatg
      361 tggtcatcct gatttccgga cccgaatcgg ccgaacaagg tatgagtgtg aatatcgatt
      421 gcgctttggc aacggaaagt atgtttatcg cggctttggg cctaggcctg ggcgcccgca
      481 tatacacagc acctatcgcc ggggtgaatg ccaggaaaca ggattttcag attcccgaag
      541 gctacagggc agttaccgtc ttgaggatag ggcatatgga taagaatgtt gatgcggtta
      601 cggcggcttc accgcggaag gcgaaagctg agctggtaaa tgtggtggaa taagcagaaa
      661 aggatgaagc tattgttgtt gggttccgga gggcgcgagc acgcgctggc ctggaagctt
      721 gttcaaagtc caaaagtaga gcgtttattt attgcgccgg gtaatgccgg tacttccgaa
      781 gtgggagtca atgtgcctct tgcggtagat gattttgccg ccatccggga ttttgctctc
      841 aaagaacaga tcgatatgct ggtggttggg ccggagattc ctctggttaa aggcatcagc
      901 gattttttcg cctccgatcc gctaacggcc ggtattccgg ttatcggtcc ttccaaaagc
      961 ggggcgcgtc tggagggcag caaagatttt tccaaggcct ttatgaaaag gcacaacatt
     1021 cctaccgcag gctatttgag tgtgacggca gagaatgtac aggaggggct cgattttatg
     1081 aaaaagctgc ggcctcccta cgtgctcaag gccgacggtt tggctgccgg caagggtgtt
     1141 ttgattattc aggatctcga agaggctcaa aaagaattga aaaccatgct cgatggcaag
     1201 tttggagagg ccagcaagac ggtgcttatt gaagaatttt tgagcggcat agaatgttcg
     1261 gtttttgttc ttaccgatgg ccgtcaatac aagattttgc ccgaagccaa agattacaag
     1321 agaataggcg agggcgacac cggactgaat acgggaggca tgggttctgt ttcgcctgta
     1381 ccttttgccg ataagctttt tatgcaaaaa gtggaggaaa gaattattcg tcctaccatc
     1441 gagggtttgc gtaaggaaaa catcgattat aaaggtttta tctttttcgg attgatcaag
     1501 gtagacgaag aaccttatgt gattgaatac aacgttcgta tgggcgatcc agaagccgaa
     1561 accgtcgtac tcaggattaa atccgatttg gttgatttgc tggagggggt ggcccggggc
     1621 aatctcgatg agaaaaaaat agaattcgac ccgcgtacgg cggttgccgt gatgctggtt
     1681 tctggtggct atccgggaga cttcgaaaaa ggtaaagaaa taaccggact ggacagggtg
     1741 aaagaaagca ttgtgttcca cgccggtacc aaaattcagg acggaaagat actgagctcg
     1801 ggaggcaggg tgctggccgt cagctcttac ggcaacaata ttgcggaggc cttatcgtta
     1861 tccttcgaaa atgcccgcaa gatccatttc gaaaactctt atttccgttc ggatataggc
     1921 ttcgatttat agagcaaacc ggccgcttgt ttttagtttt tgagcatgag cagaagaaat
     1981 tatatccggc atctttcgcg taccaatctc ctttgggtcg tcggtctgtt gctttgtgtg
     2041 ctgggggtgg tctttagtac catgctgctt acgcccggga ctgaagtgct gtattctgat
     2101 gaggtgatca gtccgttttg gaagccggtt cgcggtttta tacccgggga gtggttgccg
     2161 gtgttgatac aattcctttt gttggcggcc aatgcgatcc tgtttcaatt tattgttcgc
     2221 agatacgaat tgctgcgcac gttgggcgtg cttacttttt ttatttattg ctttattacc
     2281 gcagccagca tacccttgca ttgcctgatt ccgggttctt ttgccgccct gttcataggt
     2341 ttagcttact tcagtatttt tgagagttac cgcaaagaaa attgtaataa tcaggtcttc
     2401 aacgcttctt ttttgattgt gttggcggct ttgtttgttc ctccgcttat ttttttgctt
     2461 cccttgtttt ggctggcttt caatatagtc aataccatga acttcaaaag atggctggtg
     2521 tcgctcatgg gtgcattggt tgtgttttgg ttgttgggcg gagtcttgtt tctgagcgat
     2581 cggttggagc tgattccggc cttttttgag gcatgtatac aatacggttg ggtgttttct
     2641 gccgagttgc ctactatggg cattgttttt ttctctatgc tttttttgct ggttatgttg
     2701 gctattgtca actacttgcg gcaggtgtat tccgaaaaaa tcggtgtgcg ttcggctttt
     2761 tcctttttgc tgatctcctt gttggtcttt gttgtttcca tggttatttt tccggcttat
     2821 aaagtcgatt tctttttatt aagcataggc cccgcatcgg tcatgttgtc tcattattac
     2881 gggtcggaag tgaacttgtt tggccgtttg gctttatatg tttttctttt gacggccgtg
     2941 ggcaattatt tgtctttttt cttttccttt tgatcgttct cttggcttgc agttctccgg
     3001 tatttttccg ctttctgagc aggggtacat tattctttgg tggcccggat gatctcgcgt
     3061 tttcccggag gcccttgcag tctttccacg ccgaagcccg ctctttgtaa tcttcttctg
     3121 atttcgcctt tggcgcaata actgaccaga atgccccctg ttgtcatgtg tttatacatg
     3181 gaggcaaaga gttcttctgt ccacattccg gcttgttttt cgggcgaaaa aggatcccag
     3241 tacaccaggt cgtaactctg cggcagttcg aacaaggcaa aatctccacg gaacttggtg
     3301 aatgttgtat gtgaagccgg ttctgtgtgg cgaacattcc attccgcggc atgaatgaaa
     3361 tcgagaacgg cagcagcctt gttttgatct tgctccgaaa acagggccgg atagttgaga
     3421 gccttgatca ggtcgggaga tagagggtag gcttctatgc aatcgtagct caagttcagt
     3481 tgcagcttcc cggcttccag tatggttagc aaggcgttga ggccggttcc caggcctagc
     3541 tcaaaaacct tcagggatgg atttgttccg gcggttttac ggcggtatgc ttcgagcagg
     3601 ccggcctgaa taaagatatg gcgtgattct tgcagagcgc caaaagtgga atgataatgt
     3661 tcctgcaccc tgtttgaata cagggtttgc gagccgtcgg cagtcgggca gatttcgcag
     3721 gacttagcgg gaatgaattc gttttgttgt ttcatgctca gccggccact gtagagggaa
     3781 taccggttgc atcccgcagt cggcgggcga tcgtttccag ttctttcagt ggaatttttt
     3841 cgaggctttg caccggggtt tctctgtcga gggtgtagat catcaccgaa aggggtttga
     3901 ttttgcgcag ggcttcgatc caggcagtga tttcttcttc ggtggtgttg tctatgattt
     3961 tgcccaggaa ggttccgcgc agaaagagag tctgaacgat cagcttccct tcaaagagct
     4021 gcatatattc aatttgttgc tgaatggaat aagccttttt aaccggccgg ttcagccatt
     4081 gtacagtcga ttccaaagcg gaatctaact ttagaatatt gttgtccacc tttaacaggg
     4141 ctgccctgat ttctgctttg tctatccggc ttgaattgga caaaacgctg actttagcct
     4201 gaggaaccat acgatcccgc aacacaacaa ctgcatcgac gatggccgga aagtccggat
     4261 gcaaggtggg ttctccgtta ccggcaaagg taaggacatc gggaacaact cccttggcca
     4321 gcaattcctg aagtttgagg tcgagagcgg caattacttg ttctgttttc ggaagaatac
     4381 ccctgttttt catgtcgccc agcccgcatt cgcaatacag gcagtcgaaa ctgcaaaact
     4441 ttttgctcaa gggtaagagg tttataccca gtgaaatacc gagtcgtcgg ctcttgaccg
     4501 gaccgaatat aatatcttga aacagcatgg tggacatggc ggtttaggag tttaggggat
     4561 aagtatgtcc cagttcgttt ttgttcggat agtcgatggt ataatgcaag ccccggctct
     4621 ctttgcgtaa ctgagcctgc ttgattacca gataaccggt gttgatgatg ttccggagtt
     4681 cacaaatttc cttggaaaca accgaacgta taaagagcct ttcggtttcc tgatagagaa
     4741 tttccagacg gtccaaagcc ctcttcaacc ggaggttgga gcgaacaatg cccacatagg
     4801 aactcataat ctgacccact tctttggcgc tttgggtaat cagaatcatt tcttcgggta
     4861 gccgggttcc ttcgtcgttc cacaggggaa ttctttcttc gaagcctatg tctttcaggc
     4921 gtcccagagc gtgtttggca gccgaatcgg cgtatacgat ggcttctatc agagaattgg
     4981 aagccagccg gtttgccccg tgcagccctg ttctggcgca ttcacccgag gcatacaggc
     5041 ggtctatgct cgattcggcg tttttgtcga cctcgatgcc gccgcataaa taatgggcag
     5101 cgggagccac cggaatatac tctttggtaa tgtcaacgcc gatagacagg cacttcttgt
     5161 agatattggg aaaatgtttt ttggtttcag ccggctcttt atgggtaacg tccagataaa
     5221 cgtaatcggt accccggatt ttcatttcgg tgtcgatggc gcgggcaacg atatcgcggg
     5281 gagccagtga agctctgtcg tcgtattttt gcataaattc ctctccgtca cgtgtacgca
     5341 atacggcgcc gtaaccccgc atggcttccg taattaaaaa agagggtctt tcgcccggat
     5401 gataaagagc agtaggatga aactgcacaa actccatatc ttttacctgc ccttttgccc
     5461 ggtagaccat ggcaatgccg tcgccggtag caatcacggg attggtggtg gtttggtaaa
     5521 tattaccaat gcctccggtg gccatcatgg tgactttggc caaaaaagta tgtatggctc
     5581 tggtacgccg gttcaacaca taagctccgt aacattcgat atgttcggat tgcctggtta
     5641 tggtggctcc caaatggtgt tgggtcagta tttcgatggc aaaatgatgg tcgaaaacct
     5701 ggatgttggg atgttgttgg atgcggcgga tcaggctgcg ttgaatttcg gctccggtag
     5761 cgtcctggtg gtgaaggatg cggttttcgg aatggcctcc ttcgcgatga agatcgtaat
     5821 gtccgctctg gtctttgtca aaggcaactc cccaatggat cagttcggcg atctgggcag
     5881 gagcctcctt taccaccttt tccaccaccg atttatcgca tagtccgtct ccgcagatca
     5941 gggtgtcttt tatgtgtttt tgaaaattat cggttgccgg attggtgacg gaggcgatac
     6001 cgccctgagc ataaaaggtg ttggcttctt ccaaagccga cttgcagcaa atggccactg
     6061 tccctttgtc ggccaccttc aaagcaaagc tcattccggc caggccggag ccgattacca
     6121 aaaaatcaaa tttgcgtacc atagcaacac cattgtttgt ggctcaaaaa tccgaaataa
     6181 cctacttatc gcaggaaatt ggactgcaaa agtaatcttt ttttcatcaa atctttagta
     6241 cctttgcagc gttttttaga aagcgaaaat ggagtacaat ttcagcgcca tcgaaaagaa
     6301 gtggcaggag gagtggatta agcgtcaaac atacaaagtc tacgaagatc gatctaaacc
     6361 taaatactat gtgctggata tgttccctta tccttcggga gcgggtttgc acgtggggca
     6421 tcccttgggc tacatagctt ccgacatagt ggcaagatat aagcgattgc agggatacaa
     6481 tgtgttgcat cctatgggtt acgatgctta cggcctgccc gctgaacagt acgccattca
     6541 aaccggccag catccggcca ttactacagc caataacatt gagcgttaca gggagcagct
     6601 cgataaaatc ggtttcagtt tcgattggag ccgcgaagtc agaacctgtg agcctaagta
     6661 ttataagtgg actcaatggg cttttattca aatgtttaaa agctactaca atacagaact
     6721 ggatcgggcg cggcctattg aagaattgat tgagctcttt gagtcgcacg gcaataaaga
     6781 catccccgct tacggaggtg aagaattgcc cgaattttcg gctgctcagt ggaaaagctt
     6841 tgataacctc aagcaacaag agttgctgat gcactaccgc attgcctacc tgggcgacag
     6901 catggtgaat tggtgtccgg ctttaggcac ggttttagcc aacgacgagg taagcgaagg
     6961 cttgtcgttg cgaggagggt atccggtgga acaaaaactg atgcgccaat ggtgcttgcg
     7021 catttcggcc tacgccgaac gtttgctgca aggtttggaa aaaatcgact ggaccgattc
     7081 gctcaaagaa acccagcgca attggatagg tcgttccgaa ggagccgaaa tgaactttgg
     7141 ggtcaaagat tcagacctga ccatccccat ttttaccacc cgtgccgaca ctattttcgg
     7201 agtaacattt atggtattgg ctcccgaaag cgaattagtg gcgagcctta caaaacccga
     7261 atataaggct ggcgtggacg cctatcttca gaaagtgaag aaacgcaccg aacgggaacg
     7321 catagccgac cgccgggtaa gcggtgtgtt taccggcagt tacgccataa atccgttgtc
     7381 gagagccgaa atccccattt ggataagcga ttacgtgctg gccggctacg gcaccggagc
     7441 cattatggct gttcccggac acgacagccg ggactatgct tttgcaaaac acttcgatct
     7501 gcccattatc cccttgattg agggttgcga tatttcggaa gaaagtttcg atgccaaaga
     7561 aggcattatg aagaattccg gctttttaaa cggattgagt gtgccggaag ccattgaaaa
     7621 aacaaaagcc taccttatta agaaccaact gggacggatc aaagtgaatt accggctccg
     7681 cgatgccata ttcagccgtc agcgttattg gggagagcct tttccggtct attacaaaga
     7741 cggtttacct caagtgttgg acgagagctc tttgcctctg gaattgcccg aagtggataa
     7801 ttttctgcct accgaaaaag gggaacctcc gctgggtcac gccaaaggat ggaaaaccga
     7861 agagggttat cctttggaat tgaacaccat gccgggtttt gccggttcgt cggcctatta
     7921 tttgcgttac atggatccgc ataacgaaga ggccctggtt tcggtagaag ccaatactta
     7981 ctggagacag gtcgatctat acgtcggggg tacggaacac gccaccggac acttgctgta
     8041 cagccgtttc tggaataaat ttttgtacga tctgggggtg gtatgtgaag atgaaccttt
     8101 caagaagctc gttaaccaag gtatgattca aggccgctcc aattttgtgt acagaatcaa
     8161 agacagcaac acttttgttt ccaacaatct caaagacaag tacgaataca ctgccatcca
     8221 cgtggatata aacattgtaa acaacgacgt gcttgatttg gagcgttttc gcaactggag
     8281 tcccgactac gaagatgccg agtttatttt agaagacggc aaatacattt gtggctgggc
     8341 catcgaaaaa atgtccaagt cgatgtacaa cgtggtcaat cccgatcaga tcgtcgagaa
     8401 atacggagcc gacaccctga ggttgtacga aatgttcctg ggtcctctgg agcaatccaa
     8461 gccctgggat accaacggga ttgacggcgt gcaccgtttc cttaaaaagc tatggaatct
     8521 ttgtgtttcg gaagatgagt tcagtttcag cgatgcagct cccgacaaac aggaactgaa
     8581 atcgcttcat aagttgatta agaaactgaa ttgggacatt gaacattttt cgttcaatac
     8641 ggccgtcagc gcttttatga tctgtgtcaa tgaattgtcg gcaatgaaat gcaacaaaag
     8701 agacataatt aaacccttga tcatcctttt ggcccccttt gccccgcatt tcagcgaaga
     8761 aatctggcag gcgctcgggg agcagacttc tgtttgcgat gccgtctggc ctgaattgaa
     8821 cgaagattac ttagtggaag atcaggtgac ttacgccgtt tctttcaacg ggaaaacccg
     8881 ttttatgctg gacgtttcgt ccgaagccgg caaagaggaa gtggagcaat tggccctggc
     8941 tcacgaaaac agtcaaaagt ggctcgaagg caaaacgccc aaaaagatta ttgttgttcc
     9001 ggggaagata gtgaatattg tagtatagtt ctcagtcgga tagtattcct atgataatcg
     9061 attaagtcaa gccaacaaac caaaacccgg ttgagctcga tctgaacgga cagcccgaag
     9121 gcatttacct gattaggccg agttccgacg ggcaaaaagt gttgttgaga ttgatgttca
     9181 gagaggaaca ggcagcgttt acaatacaac gctaggccag ccctgatcat cccatttgat
     9241 ttcgcgtatc agaaggatag agcggccgtt gcgttccatg tcgtagccgt gaaagaacag
     9301 gtaatctttg cccccaaagc tggctacggc attatgtccg acaccgggga aacgctcgtt
     9361 gcctttgact actaaggttc ctgcgccttt ggtcatcacg ttgccttctc tgtccacgta
     9421 aggaccggtc acttttcggg aacggcccac cactacgttg taggtgcttt gggctccgcg
     9481 gcagcacata tcaaaagaaa caaagagata gtagaaactg cctttgcgga aaataaaagg
     9541 ggcttcgacg gctcctcctc cgggttctac atcctggtcg ttgatgaggc gcggtctgcg
     9601 cgaaagcgga taccattcct gaggctctgc cagggcgcag aggctgctgt cgagtttgac
     9661 cagctttata ccttcccaga aagagccgaa gctcatccag cccgtaccgt cttcatctat
     9721 gatgatgttg ggatctatgg cattccacat atcgcggttg ggaatggagc gcaacaccat
     9781 gccctgatct tcccatttga aatcgggcga cccggggtcg agcgtcttgt tggtggcaac
     9841 gcctatgcag gaattgtttt tgccgaaagt ggataccgaa taataaagat aatacttgcc
     9901 ttggtgctta ataatatcgg gcgcccaggt atgtcccctg taacccggca ctgcttcaac
     9961 agcccaagcg ggaggctccg aaaaaaccgg tttctcgcgt ttccagttga tcatgtcggt
    10021 cgaagaaagg acggagatgc caaatccggt acaaaacaaa taataggtat tgccctgttt
    10081 ggccactacc gggtcgtgga cggagggagt ggaactccct tccgggttaa aacgattgtc
    10141 ctgggcggaa agccgcagtg taaaactcaa taaaaaagtg atgcttacaa cgaataaagc
    10201 gtttttcata ttatatggga taaaaagatt cagccctaag tcagggtact gtcaacgagg
    10261 ctcaaaaata gctatttttg gtttaaacga aaactgtgaa gcaaaaatgt ggggattttt
    10321 ttgcaagaca taaaaagccg acgtactttt gcggggtctt atgtggaagg atttggacgc
    10381 ttgcaaccac ttgaaaaaat gctaaagaat catcttcttt tctgcaatct cccctgactt
    10441 ccaagacttt tattaagtgt caccggcccc agatcctgcc ggttcagtgt atttgcgggc
    10501 attaaaacag atagaacgat gggatattta tttacttcag aatcggtgtc cgaaggacac
    10561 cccgacaaag tagccgatca gatttcggat gctttggttg acgcctttct ggcttacgac
    10621 gaaaattcca aaacagcttg tgaaactttg gtaaccaccg gtcaggttgt gctggcaggc
    10681 gaagtgaagt cgagcattta tgttgatgtg caagaagtgg ccaggcaggt tattaaaaag
    10741 atcggttata ccaaaagcga atacagattc gacggggatt cctgtggggt gttttcttcc
    10801 atacacgaac aatcggaaga catcaaccgg ggcgttgacg gtgccgctaa aatcgatcct
    10861 atgaaccagg gtgccggcga ccagggtatg atgtttggtt atgcctgcaa ggataccgac
    10921 aattacatgc ccctgccctt ggagctttcg catcgcctgc tcagaaagtt ggcagacatt
    10981 cgtcgcgaag ggaaagtaat gacttacctg cgtcccgatt ccaaatcgca ggttaccatt
    11041 gaatacaacg acaaaggaaa gcccgaacgg attcatacca tcgttgtttc cactcagcac
    11101 gacgaattta ttccggccgg caaaggactc agtcaggaac aagccgatgc ggagatgttg
    11161 agtgtcatcc gcaaggatat tcagcaatac ctgctgcccg cagtttttaa cgccgatccg
    11221 ggcctgaaag ccttactcga cgataaactt attctgcatg taaatcccac cggtaagttt
    11281 gtgataggcg gccctcacgg cgacagcggt ttgaccggcc gaaaaataat tgtggatact
    11341 tacggcggta aaggagccca cgggggaggt gctttctcag gtaaagatcc ctccaaggtg
    11401 gaccgttcgg ccgcctacgc tgccaggcat attgccaaga acctggtggc tgccggcgtg
    11461 gcagacgaaa ttctgatcca ggtatcgtac gccattggtg tggcacatcc tgtaaatatt
    11521 tttgtcgata cttacggtaa gtctcgtgtt aaaatcagtg acggagagat tgcagaaaag
    11581 gtggtccagt cgtttgatat gcgtcccaaa gccatagaac aacgcttgaa actgcgtaaa
    11641 cctatctatt ccgaaacggc ttcttacggg cactttggca gagaacccca gaagctggtc
    11701 aaacattttt cgtcgcgcta tgaaggagac aaaaaaatag aagtggaatt gtttacctgg
    11761 gaaaagttgg attacgtgga gcagataaaa aaggctttcg gtttataaca ttccctgttc
    11821 aaccataatt attattcgct caatgagtcg gtcggtctga ttatcggtgg gattgtagtc
    11881 cgttttaagg tctgccgtaa acagtttggc tacgccctgc cagaaattgg ccgcaaaata
    11941 acccctatag gctgcgatta tttcgtaagg cgaagaatcg cattcacgta cgatgagctt
    12001 tagcaggatt tttccctggc tatagctcat cttgcgtaat gtgggttcat aatctttgat
    12061 cagatcgcgc tgcatttgtc tcaggtgttt ttcgcgggct ttgtcgtcag ggagtgtttc
    12121 caggtattcg gccgtttcga taagggtgcc cgagactatc cgggccagag ggaggcacag
    12181 ttttacatcg cgtaccaatc gcgaatagta gcgctcttca cgtttgtttt tgaacaccat
    12241 gcccggataa acatagacgg gattaagatt caccagcaac acagtgtctc cgccttctat
    12301 ccgtgcagga atcgactgcg gaggtcgcgc aggaagcagc ctgtttatac cttcgtcaat
    12361 ttgtgcccgg ctgtttatgc ttaacaaaag gaacagcgta aacaaaagat agcgcataca
    12421 attcgtttct ggttttgcaa aagtacctct ttaaaaccaa gtagcaaata aatggaatta
    12481 ttgaacggga agtgtttatt tggcaacaag taaatagtaa agaatcaaac annnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nattattgaa cgggaagtgt ttatttggca
    12661 acaagtaaat agtaaagaat caaacaacag acgttaaatt tgcatcaatt aattttgatc
    12721 gtcatgaatg ttttgagatt ctttaagcgt agatctgcag ctccggatcc gtctgttgtc
    12781 cggggaaaga ataatatctt gtggtattgt gccttgataa taaaggtatt gcattcgtcc
    12841 ccgggacttt atgcacaaac aatttccgcc cgcgaatggt atggagatgc caagagtatg
    12901 tctatgggcg atgccatgct catttcgcaa tggctttttt cggagccttc ccatcaaaga
    12961 caacaagata acatgttggg tgtacagcgt gctcaggcca cctattattt gccttttaac
    13021 cgggcttcgc tggcggtcac cggcctgatg attcagttga gatctcctct ggccgattta
    13081 cagttgaata tggggcgatc gggcggggac gattacaacg agagcttgat cagtctgggt
    13141 tgtgcacgct caccgacaga aaatcttagc gggggcttcc gtctggggat atattctttg
    13201 gccgggaatc agcttcatga gggaacggct cttctgacgg aatgttttgt gtattacctt
    13261 gtcaatacac aatggaggct gggggcttat atgttcaatc ccatagcagc caaagtttcg
    13321 ggtatacccc tgattcaaag ttatcacctg gcctgtgcat atcgtcccga ttctcgtctc
    13381 gatttcgtat tggaaatgga aaaaccccaa agacaggaca ttctggtgca tttgggaatg
    13441 gaagctgccg ttttgaaatg ccttcgatgc aggctgggtc tcatttatcc ggttatgcag
    13501 ccttcgtttg gcctggcctg ctcttttaaa aaattcagtt tggaggctgc ctgccgtctg
    13561 cataaacaat tgcccgcttc ttatgctgtc acgctgcatt atacgccttg attgcctgct
    13621 tttgcttatc cttatttctt caaagctgct tagtgcgcag gaatcgtatg atacggatga
    13681 tttggagcat attctctcgg attgggtaga acaaaatccc gaagccgatg ccgaagccct
    13741 ggctcaggaa ttgtatgaat tgctggaaca acctgtcaac ctaaacgacg ccggcagaga
    13801 acaattggaa tctttgcctt ttttgagtcc ggatcaaaaa gaaaaactgg cctatttttt
    13861 ataccgtcgt aaacctttgt actctttgtc ggagttgctg ttgtgcgaag gtatggacga
    13921 ggtgagcttc aggcgtatcc gccctttttt gtacatcggc gcagctgacc aaacctcaga
    13981 aaagctcgat tttaaaaagc tcctgagctg tggaaagcac gaaatcaggg cctacacggc
    14041 tgccggtttt caacctaggc aggggctgct taatgccggc gattccgcct cgaaagccga
    14101 agggaaagct taccttggac tgcccttttc gggagtttgc aaataccgct acagctatgg
    14161 gcgtcagttg cagtacggtt tggtaatgga acacgatttg ggcgaaaggc ccgtggactt
    14221 tttgtctttt catatagctg ttcaccaact ggcaaagatc aacactttaa ttctgggaga
    14281 ttatcgcctt aaattgggcc gtggtttgct ggtttcttcg ggtttcagtt cgggaaaaac
    14341 cacttccccg tctgcgttgg catccgttcc aaaaatgctg agcagccatt tttcctgttc
    14401 cgaaagtgct tattttaggg gcgccgccat cgatctttct ttgtcggaac atcttcaatt
    14461 actggctttt gcctcaggcc gtaaactgga tgccgtgctt gaagagggca gttttacctc
    14521 tgttcgcagc gacggcttgc accgtacttc gaaggagctc agttacagga atgctttaag
    14581 gcaatacagt accggggcgc acatcggcat taatcaaagc tattttcagg ccggagccaa
    14641 tgtgctttat tacaggttca atgctttctg taatcccgac ctcaaaccct actctctgtt
    14701 ttattttaga ggcagagaca acctgaattt ttcgctcgat taccgttttc gcgccggaac
    14761 ggccctgttc agcggtgaat gcgcttttga taataaatgt catacggccc agttccatca
    14821 gttgcaatac cggcctcatc ctttgctgaa tatggttcac tcttttcgtt attacagccc
    14881 ggcttaccag gcttattttg cggcagcttt ttcggaaaac agccgggtgt gcaatgaaac
    14941 cggctggttt accgctatgg aataccgttt gtttgagcgt ctgaaaatgg acgcttacct
    15001 ggatgtattt gtctttccct ggttgaaata cggagtcaat gccccttcgt cgggtcacga
    15061 ccttgggctg gaactcagct cttgcatcgt aaatggtctt gaatggagcc tgcgttaccg
    15121 ggaaaaaact aaaacagcaa acttgccggt cgaaggaaga aaaggcataa caccatgtgc
    15181 cgacaatact aaaaggcagt tgcgtctgca attaaaagcc ggttttggag gattaagcct
    15241 gaaaacaaaa gcggaactca accggtttaa tgttgccggc tttgaaacat ctacgggtgc
    15301 gttgttttcg caggaatgcc gttataaaac tcccgatgag cggctcagtt tatctttgca
    15361 atacgccatg tttgataccg aaaaatatga aaacagaatc tatttttacg agtacgatct
    15421 gccgggtgtt ttctccattc ctgcctacag tggtacgggc agtgcttatg tattgtgctg
    15481 caagctgaag ccgggccgca attttcaatg ttgggcgaga ctcaggcacc gggcttattc
    15541 cgaccgcgag gaagtgggga ctatgttgga catcgtgcgg ggcccttgtt caaccgagtt
    15601 tcgtttgggt tttacgtatc gtttttctgc caaaagcggc ttaagcggtc ggaatcaacc
    15661 atagtacagg cttgtgtttg gatttgtttt tctatctttg ccgcttattg atgcgataat
    15721 tttatctttg tcccaaattc aacattatca ttcggggttg atcgctagcc cttttgcatt
    15781 gtaacaacat gaaaaatttc aagcttttaa acaacgtatt cggttggttc gtttttttgg
    15841 tggcagccgt aacgtattta ctcaccattg aacctacgac cagtttctgg gattgcggtg
    15901 aattcatttc ggcggcctac aagctcgatg tgggacatcc tcccggagct ccgtttttta
    15961 tgttgacagg tcgcttcttt gcgaatttcg ccagtggccc cgagcaggta gctaagatga
    16021 taaatgccat gtcggcattg tttagcgcct ttactatttt gtttttgttt tggactatta
    16081 ctcatttggc cagaaaagta gtcgttaagg acaacgataa tatgaagccg gcagaaatga
    16141 ttactgtttt gggtgccggt ctggtaggtg ctttggccta cactttttcc gatacttttt
    16201 ggttctcggc cgtggagggc gaagtgtann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16321 nnnnnnnntg gcctacactt tttccgatac tttttggttc tcggccgtgg agggcgaagt
    16381 gtacgcctat tcttcctttt ttacagccgt agtcttttgg tctatactca aatgggaaga
    16441 agaagccgac aaaccgcatt cactgcgctg gattattttt atctcctatc tgatggggct
    16501 ttccataggg gtgcatttgt tgaatttatt gtgtatcccg gctttggtgc tggtcgttta
    16561 tttcagaaaa aatcaaaatg ccaatgccaa aggagccgct cttgctctgt taggctcttt
    16621 tgtcctgatc gccatagtac tttacggtgt ggttcccggc tttgtgaata tggccggctg
    16681 ggccgatctt ttgtttgtta atgtgctggg ttttagcttt aactcgggag ctgttgccta
    16741 catattagtc atgatcgctg tcctttgttg gggactggca gaaagctacc ggggtaagaa
    16801 tgaattcagg atgaaactgg ctttcgcttt gaatatcgta ttgtccggta ttccgttttt
    16861 cggcaacagc ccctgggtat ggattttgct ctgcggattg actctcgcag tggttttcag
    16921 gtggaagaaa attaacggta agctcatcaa tacctcttta ctggcgctga tggtgatgct
    16981 tatcggttat ttcagctacg gggtcattgt gattcgttca aatgcacaac ctccgatgga
    17041 tcaaaattct cccaacaata tgtttaccct cgagcgctat cttaaccgtg agcaatatgg
    17101 cgaaactcct ctgatttacg gacaaacttt tgcctcggac ataaaatgga aaagacaagg
    17161 caatatttgt gtccctcaat acaaaaaaag aggcccggtg tggagcaagc aggttaaaac
    17221 ttcgcccgac gaaaaagatc gttacttcat ttccggctat aacgaacgcc cggtgatggc
    17281 tgatgaattg aatatgttct ttccgcgtat gcacagcaat aaaccggaac atgtggtgat
    17341 gtacaaggat tgggttaatt ttaaaggaac tcccgtcaag tacaataaat gcggtcagat
    17401 tgaaaccgtt tacaagccta ccatggccga aaacctgcgc ttcttctttt cgtatcagat
    17461 taattttatg tactggcgct actttatgtg gaatttctcg ggccggcaaa acgatattca
    17521 aaacctgggg ggcgaggcca acaacgggaa ctggattacc ggttttaatt tcatcgataa
    17581 acacctggtg ggtgatcaga gcaatcttcc gccggaactg gccgataaca agggtcgcaa
    17641 ccgttattat atgttgccct tgcttttggg aattctgggg cttttgtatc aggcctatgc
    17701 cgggaaaaag ggcgtgcagg gattctggat taccttgctt ctctttttga tgaccgggct
    17761 ggccatagtg ttgtatttga atcagcctcc cttgcagcct cgcgagcggg attatgccta
    17821 cgccggttcg ttctatgcct tttgtatatg gataggtatg ggtgtgccgg ctctggtttc
    17881 cattctgcgc aaagccctga agccggtacc gtcggcattg ctggccgggg tgctcgggct
    17941 gggtattcct gtgcttatgg cttccgaaaa ttgggacgat cacgatcgtt ccgatcgtta
    18001 tacggcccgt gactttgcct ataattacct gataggttgc gatgaaaaca gcatcattta
    18061 cactaacggc gataacgata ccttcccctt gtggtatttg caggaggtgg aaggcataag
    18121 aaccgacgtt cgtgtctgca acctgagtta tttgcaaacc gactggtact acgatcaaat
    18181 gttgaggccc tattacgagt cggatgcttt cccggtgagc tgggataaat cgaaatacag
    18241 caacggtaag cgcgatattg cccgtgttat tccccgtacc caggatacga tcagtctgcc
    18301 cttcgccatg aactggctgg gtagtgatga cgcaagcacc aagcgcgtgc agggatacgg
    18361 cgataatgta gatcacattc ctgccaataa gctctatttg gatgttgatg cagaaaaagt
    18421 gcttgaaaac ggttacctgg agcctaaata cgctgaacat atcgtgccgc gtatgttcat
    18481 cgatttgtcg gctaagaatt acctgggtaa acacgagatc atgacgctca agttgctcga
    18541 ggaaaacaat tgggaaagat ccatgtacta tgccgtaacc gtagaccctg cactaatgac
    18601 catgttgcaa cggtatttcc tgcatgaagg gcttatttat aagcttgcac cattggaaag
    18661 caacagggca ataaacactg ataaaatgta cgacaacatg gtaaataaat accgttgggg
    18721 aggtatcaag gagaatcccg atatttacct cgacgaaaac aacctgagaa tgtgtcgcac
    18781 tcacaggcaa cactttggtt ttctgatcaa ggccctttat caggaaggta agtacgatat
    18841 ggctctgcag gctatagata aatgcttgga agaacttccc actgagcacg ttcctgtcaa
    18901 ctttatcgat ggaggtatgg caggaatgct cgaaatcacc gaagttttat atgacctggg
    18961 agaaaaggaa cggtctttgg ccattgcaaa cgaaggaatg gatctttgta ttcagaatct
    19021 gaattggttc ttcagtttga atgatcctct tttgcgtgct tccggcagat cggtcaataa
    19081 tcaactttac gtgatgcagg aacttaggaa ttttctccag cgtgccgcta ccgaagcctc
    19141 cgctctggaa aatccttcca aagtcgatgt cgcctttatg gaggccttta ataaaaatac
    19201 gcaacacttt ggccagtttt atcagcaata ccagcggctc agataattag ctatgctgtt
    19261 tacgcaatta agtcgttgct tgcattggat accgggaatt acctggcgaa agtctgtgtc
    19321 gcaaaaaacg atttacctta gttttgacga cggtccgata cccggtgtaa caccttgggt
    19381 attggacacg ctcgatcgtt acggcatcaa agctactttt ttttgcgtag gcgagaacgt
    19441 tgcccgttat cccgaattgt ttgagcaaat caaaaccaga gggcatcaaa caggtaacca
    19501 ttcttacaac cacctgaggg ctttcagaac aaatccggac gactatctgg ccaatgttga
    19561 aaaggccgac aaactgatag ggagtagttt gtttcgtccc cctcacggcg aattgactta
    19621 cgctcaattc agagctctcc ggaaaaaata cgagttggtg caatgggacc ttatttcccg
    19681 ggattacaat gccaaattaa gccccgatga ggtttttgac attgtacgta agggtgtcag
    19741 aaaaggttct atacttgttt ttcacgattc gctgaaagcg gagaaaaatc tcagggctgc
    19801 cttgcccagg tccatagaat tttgtctggc gcaaggttat gtttttgacg tgctttgacg
    19861 ttggacttgt tttttcgagc ggcggtttcg atgtttccga ttgtatttta atacctcttc
    19921 gacacatatg gttttgtcga aggagcgcac ttcggcgtat tcttctgcaa tccggctaag
    19981 gatttcgtcg ttcagacaaa gtcgttcaaa atttttacag ccttttttac ggcctactct
    20041 tccaaagcgc atgcggttca gatctattac ctgcaaaagc acttcgtctt tattgatttc
    20101 gtacaagaga tttcccgaag aataatcttt atgtaaaata cccagatcgt ggagtttagc
    20161 tgtaaaaacg gcgatacctc ttaccagctt atcgacatct tttgccggag tttcggtaca
    20221 aaggtcgcta aggcgattgg ggaaagtcga tttcaggctg atgaaaaaac tcttatgtaa
    20281 aatcaggcct tttttaatct caatataggc caggggttga ggagtaccga taccgtaatc
    20341 cctgagtttc agggcatagt tgtaagccct tttggctttg gaggctctga tgtttccgta
    20401 aataattctg ttgataaaca agggattacc ataggacttt actacaacat ccatgccttc
    20461 aacgcggtaa ctttttatgg tgtttctggc ttgaaacatt aaatctcccg acgtatcgaa
    20521 ttgttcgggc agagaggaca ggaaggggtg caggtttttg taatcctcgt ggagaactag
    20581 ttttgtgcgt tccagtaagt tgtttattga attaaaaaat ttggaaaagg ttctgttata
    20641 atgcagtttt ccctttagtt ctcttttgaa aaaatccgcg tggcgctgat tcagaatatc
    20701 gatgatttgt ttctttttcc ccctgtcgct aattcttttt gagttgcttc tgtagcgata
    20761 ataaaaaccg gtaaagttta gttttaccac ctctccgccg tttttgagca tggatatcca
    20821 aaaatcccag tcttcgcgtc cgggaatata attgcaataa ccaccgcaag cagtccagtc
    20881 gcttttgcga tacatagcgc aggcactcag catgtttttt ctggccaaca atgctggaga
    20941 ataatccggc agcagccatt ttccggtttt atcaccgaaa aattcagccc tggcggtaac
    21001 aaccttgacc gaaggctgct tttgcaacac ttcgacggct ttttctaaga aatccttgtg
    21061 aataaggtcg tctgcatcga caggcagtat gtatttccct tgcgccagtt cgatggctgc
    21121 gtttctggct gcagacaccc cggcattggc ttgggtttct gccttgatat tggggtgttt
    21181 tgctgcgtat tcgagagcta tctgcaatga cttgtcggtg gagccgtcgt ccataataat
    21241 gagctcaaaa ggggaatatt ccgatttcag gatggaatcg atgcattccg ccagataggc
    21301 ttccatatta taaacaggga ctattacaga gatcaaaggc ttttccatag tcgtgttgta
    21361 agataagcaa ctcgttaaat tttgtcgcgc tgcttaagaa ttttcaggaa gtaatacttc
    21421 cagggccggg aatatttcaa ttgtttccac ggcctgctgc tgtgtggccg gtgcagtacc
    21481 ggcaatttgg taatcaggta attgttgaaa ccgagcttgc gtgatttatg tgagataaat
    21541 acatcgaacc aggatttgtc cggatccaga aatttaaaat cataggccga taaaagactg
    21601 ttgcttagaa tagagcagca aaaactcagc cgtttgcggg ttttgatgat ggatgagggg
    21661 taggaggagg cataaagata agggaaattg acaactccac tctcgtcaac cgtaacggcg
    21721 gccaccattc cgggattgtc gagttcagca caggcagcct gcattttctt tatggtgtct
    21781 tgtttaatgt agacatccga ttcaatgatc atcaggtctg ctcccgcttc gagggctttt
    21841 ttttgagcaa gttgcagcac cagtcggtag ttaggcgagg gattggtggt caaatcttcc
    21901 aggtgcacca agtcgaaacc gagttccaga gttaattctt cgaggagttt tttgttgctt
    21961 gccgtactga agtcattgta aatagtatag gaacaggaag gaggtaaatc cgattccttt
    22021 atggccctga tactcccggc ggcagtttca taagaatctt tgaccggggt aatgatgtgc
    22081 aaaggcttag gcatgctatt tcaaagactt gctgattttt tcggctatag ccttcatttc
    22141 ctccgctgtc ggattgagtt tgggcttaga aaaaagcata tctgtctcct tgtttatggg
    22201 gatcaggtgg atgtgtgcat gaggaacttc cataccaacc acggccatcc ctatgcgttt
    22261 gcagggtatg gcggttttaa gcgccagagc aatctttttg gcaaagacta tcatggctgc
    22321 gagctcccgg tcatcgatat cgaacagata atctgtttct tttttgggta ttaccagtgt
    22381 atgtcccggc atcaggggat gtatatccag aaaggcaaaa aaatgttcgt cttctgctat
    22441 tttgtaagag ggaatttcgc ccgctactat tttgctgaaa atactggcca taactgtatt
    22501 attttgcgtg ttcaattttt ttgatcctgt aggttataat gcctgcgggc acctcaatat
    22561 cgactacttc tccttctttt ttgccgaaaa ggccctgggc aacgggtgaa ctgaccgaaa
    22621 ttttcttttc cctgagattg gcttcgttgt cgggaacaat ggtatactga atttcctgat
    22681 ttgttttcag gttgagaaga tgaacgttgg tgaggatttg caccgtgtcg gtactgattt
    22741 tgctttcgtc cagaatacgt gcgttacgga ttagttcttt aatgttggca attttcattt
    22801 ccaacattcc ctgggcatct ttggctgctt cgtattcggc attttcagag aggtcgcctt
    22861 tgtcgcgtgc ttccgctatt tgttttgaga tgtaagcccg ttctactgtt tccaggtgct
    22921 ccagctcttc tttcaatttg tttagaccat tttcggtcat gtaatttgtt gccatagtcg
    22981 aaatccaaac tttcaaaggg attaaacaaa aagaatcccg gcaaacagcc ggaactcctt
    23041 tcaaaattag gctcaaagat atgtaattct cagtgatata aaaaattttg gccccattta
    23101 aaaatacata tagccaacca aaggaggctt ttttgtttga ccaaatcttc ggatttaaag
    23161 cagcttttgt atggcctcgt ccaaattctg tatagtttcg tctctgcctg ccaaatttcc
    23221 ctgtctgtcg ataagaaagt agctgggtaa gttgctgacg ttatacatcg acagataggc
    23281 ggagttgagc aggtttttgt cgcggacgca aatccagggt aaattcaagg ctccggtttt
    23341 ccagaaatgt tcgtccggat cgagcgacac ctggtatatt tcaaaaccgt atagttcata
    23401 cctgttgtac agctctctca tttcgatggt acgggctgcc gaatagtttg cctgaaatgc
    23461 ggtgaagtcc aacaatataa ccttgccttc gaggctggat aagggnnnnn nnnnnnnnnn
    23521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23581 nnnnnnnnnn nnnnnnnnnn nnnnngtggc tacggccgca aaatgacgat tgtctttttc
    23641 tgaaaagggg tcgaaaatca gtaatttatg tatgcgttgg aatagagcaa agtaagctac
    23701 aggcgaagac ggattggaaa aaatataccc ttgagcctgt tttttatgtt cgtcaatgca
    23761 attcaacacc agatcgatgt aggctttttc ttctatttct ttgttgttga aaagggcttg
    23821 gatggagtcg tattgtctcc gtgtattgct ttgtaataaa gcgagttctt gcatttgaac
    23881 acaatagtcg gagccgctta cccggtagcc ggtcgaaaaa gcttctgcag aggccttaat
    23941 gtttatgtgt acttgtgaat caacaaccaa aaagaggcgt tgccggtcta agcgcagacg
    24001 ataaaagtcg ggatattcgg gcagaggggc tttgaatttg aatttacccg attcgtccaa
    24061 taccacggag tcaattatgg aagtgttgct caggccaaaa aattcaaaat acaaggtctt
    24121 gttttctgca ttttcaacat ggccttccac aacgaagctt gtgttgtctg aacaagcctg
    24181 taatagtata aacaagcccg ttataaaaaa taaatttctc atttactgcg attaacgaaa
    24241 gtgtgggcga aattataaaa aattttagcc aaacagccgg gtgccaacgt tatttttgct
    24301 ccaaaagcaa gctgtgcagc ttttttacac ctttagaagc cggcaacttg atgtgaacaa
    24361 tatccgggaa ccggcataaa gaaagttctt cgggatctat cacgtacgaa gggcttcgcc
    24421 tggggactag gtgcagcaag ccggctgcag gataaaccac caatgagctt cctataacgg
    24481 caaaatagtc tgcttgtctg actatatcca tagccggatc aatcatagtc accatttcgt
    24541 gaaaccaaac aatgtgcgga cgcagctgat ctccgtaggg tgtacaatct cctattttga
    24601 cttcgtattc ctccggtttt agttccacta tgtactccga atgcacttct cctctcactt
    24661 tggtcaattc gccgtgtaaa tgcaagacat tactgctgcc ggcgcgttcg tgcaggttgt
    24721 caacattttg agtgattatt tgcacgtcga aagatttttc catagcagcc aattcatagt
    24781 ggccggcatt gggctccacc tcgaggagtt gtttacggcg atcgttgtaa aattgggtaa
    24841 ccagttccgg attggctttc cagccttcgg gagttgcaac cgcttctatc gggtaatttt
    24901 cccataaacc gccggatccg cgaaaggttt taataccact ttcggcgctc atgcctgcgc
    24961 cggtcaatac taccagtttt ttcatagttc tgcgtgtaaa aatcgggcat ggacgcttaa
    25021 tgtcaggcaa tggtcggaga agttccgccg gcacccgctg tttgcacagc ccgtaaaaca
    25081 aaccattaca aatgtaacaa atatcgcgta tatttgggcc ctaattcccc ctaacttaca
    25141 tacatggaac gaataagcag tctgcaaaat cccaggataa agcaattgtt gcgtttgcag
    25201 gccaaagcca gggagcgcag agaacaggct ctggtattgg tcgaaggagc tcgggagatc
    25261 caacttgccc tgagcaaggg atattgtccc gaacagcttt ttatttgccc cgaactttat
    25321 gctcatagtg actatcccgg actggtgggg gaaatagccg atgagatcaa aaccgaactc
    25381 tccttgcccg tcttcgaaaa gttggcctac aggaaaggat cagacggctt gctggctacg
    25441 ctcaggccca agtcccatct gctcgaagaa ctggttttgt cggcgcatcc ttttatcgta
    25501 gtgctggaag ccgtcgaaaa acccggaaac ctgggggcta tttnnnnnnn nnnnnnnnnn
    25561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25621 nnnnnnnnnn nnnnnnnnnn nnncctttta tcgtagtgct ggaagccgtc gaaaaacccg
    25681 gaaacctggg ggctattttg cgtacggccg atgctgcagc cgtggacgct gttatcgttt
    25741 gcgatccttt gtgtgatctg tacaatccca atgtggtacg gtccagcgtg ggctgtctgt
    25801 ttactgtaca gacggccgtt tgcagttcgc aacaggctct cgattggatg aggcggcata
    25861 aaatcaatat tttggccgca gaattgcaag cttcgcaatg gtatcaccag acagatatgc
    25921 gcctcagcac agccattgta atgggaacgg aagccgacgg gctgagcccg ttctggctca
    25981 ataacgccga tgaacgcatc aaaatcccca tgcggggata tatcgattct ctgaatgtgt
    26041 cggtatcgac ggctgtactc tgctttgaag ctatgcgtca gagaggattc aggtgaactt
    26101 gttcaacacg tatcgaggta tggctaaaga ccgaagccat ttcctcgaat ttctcttcag
    26161 cctcggtagt caggtaacga caactcccgc ctttggagca gagctcctca atttcgggat
    26221 gacgttgcag gtaatcgctc agactttcag ccaccaactt gccttgaacg acaatttgta
    26281 aaccgggcgg ggaatgcttg cgtatttgat tgattagaag aggataatgg gtgcagccca
    26341 gtattagagt gtcgatgaga gggtcgcgtt ggaaaagacg gtccaggtgt tgcttgacaa
    26401 aataagaagc cccggggctt tcgtgttctc cattttcgac cagcggcacc cacatcgggc
    26461 aggcttccga ctccaggtga atgtcgggga ataatttttg aatttcgatg ggataggaac
    26521 cagattgtat tgtgccttgt gttcccacca gacccacgtg cccggatttt gtcagtgaac
    26581 ccagcgcctc gaccgtcggc cgcaatacgc ccaacactct gcagttgtat tcgaggctgg
    26641 gaagatactg ttgttgtata ttgcgcaaag ccttggccga ggctgtgttg caggctagaa
    26701 taaccaaacg gcaaccctgc ccgaacaagt atttaactgc ctctaatgtg aattgattta
    26761 ccaattcgaa agaacgacta ccgtagggag ttcgtgcgtt gtctcccagg tagagataat
    26821 cgtattgcgg gagtttgcga cgaaattctt ccagtatggt cagaccgcca tagccggaat
    26881 caaatacacc tatcggcttt gcgttcatcc cggggaaaag aattatttaa gccccaattt
    26941 agccctgacc aagggcgtga catccttaca tttggacggc gaccaataca tcaggcccat
    27001 attggccgaa gtgtcgaaga tataaaccat attgagttct tcgccgacgg cttgtaccgc
    27061 tttggtcaat ttatcgttga tcgggataat cagctcctgt tgttggcgct gaatatgctc
    27121 ctgcgactcc tgataaaaac cttgtatgcg ttgttccagg tcttgaatgt cggtcatacg
    27181 gcgaatacga attgattcag gcaagctgtc tcgctgggct acaaattcgg aacctttctt
    27241 ttgatattct tcctgcataa tcagcaattc agcctcgtaa ttttttgcca aatcgtctaa
    27301 tttcttcatg gcttcggttg tttcgggcat ggcctgaaat atttcggctc tgttgacgta
    27361 accgaattcc tgagcaactg ccagcagggg caaaaaacat aagacagcaa caatgaaatg
    27421 tttatacata acgtcgtaat ttttgtattg ataataaaaa gtgttatcca actaataaaa
    27481 ggctgatagg gattaataac ccattttctt taaaacctgg tcgctgatgt ctatcgccgg
    27541 agaggcaaaa acaatattga ccccggaggc ccggtccagt attaacataa tggattgttc
    27601 ttcggccagt tccttgacag cgttgtagat ttcgtcctga ataggggtaa gcagactttc
    27661 gcgtttttta tagagttcgc cttcggaacc gaaatatttc cgcttcaaat cggccagctc
    27721 tttttctttg gccaggattt cgttctcttt gttctttttc atatcgtccg aaagataaac
    27781 catttcggcc tgatatttgg tgtatagctc tttggctgca ttagcaaggg catctacttc
    27841 ggcctgccat tttttagaca cctggttcaa ttggtcgttg gccatttcgt aagcgggaat
    27901 acgcgaaaaa atgtattcca tatcgactaa ggcgtatttt tg
//
LOCUS       DAOSTD010000020        27704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_1909_length_27704_cov_12.791385, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000020 DAOSTD010000000
VERSION     DAOSTD010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27704
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1909_length_27704_cov_12.791385"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PLI37_02390"
     CDS             <1..803
                     /locus_tag="PLI37_02390"
                     /inference="COORDINATES: protein motif:HMM:NF040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5913 domain-containing protein"
                     /protein_id="HOQ57901.1"
                     /translation="LNYVVTARARNRIKQTLKEQTQKQAEYGREILQRRMKNRKLEFD
                     EGVMTKVVRKFKYKTLTDFHAALGTEQLDLNQVIDQYVEWYQHDKIAHEAPQVTTAEK
                     YVIEPTPQQIQQAKEDILVIDQNLKGIDFTLSKCCHPIYGDEVFGFVSVTGGIKIHRE
                     DCPNAPQMKTRFPYRIVKARWAGKSGVGQYAASLEVIGKDDIGIVTNISSLISKESDL
                     GLRSIRIDSNDGLFRGYLTVVVQDAHQLEALIKKIKTIKGVKQVERGA"
     gene            complement(885..>1243)
                     /locus_tag="PLI37_02395"
     CDS             complement(885..>1243)
                     /locus_tag="PLI37_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOQ57902.1"
                     /translation="EVPLNIQAGVGLGSLFRVYGQVSPYLSYLISDKIDGAEDFYDAY
                     KDFLSNYDGKPLNSFDYGIGFGAGVEVLFLQLAVKYDFGLSEFKEVAGTVLKDVNPLF
                     SSLKNRNLSISLAILF"
     assembly_gap    1244..1343
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1536..2864)
                     /locus_tag="PLI37_02400"
     CDS             complement(1536..2864)
                     /locus_tag="PLI37_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOQ57903.1"
                     /translation="MKKTVISLLSTALLAAFSLQAQKVWTLKECIDYALANNIAIKTA
                     GLNAEIQGIEKKNTQYRRLPYVSASASHNIGFGQTQDNTGVYVNSNSQSTSAGLSTGI
                     TLFNGFQINNSVKAQQYSTLASLENLNKAKEDLSLNIASAYLQILYNKELYQLALEQV
                     DLSKEQLRQYEIRYAHDKIPEGQLYEIKAKLAQDELSATQQKQTMELSLLELSQLLEL
                     EDWKDFNISEPDSQIMVSKEALQLPAESIYDQAQSNRAAIKSAQYEVMTSEKMLEVSR
                     GARYPSLSLGASYSNSYYDHSNYANPSFKDQLSTNGRSNIGLSLSIPIFNRLNTTNNI
                     KRSNINLETARLQLENTKKILYKEIQQAWFNANTAEEKYRASFETIRATQEAFRFTEE
                     KFNSGRSTIYEYNEARMQLLRTRSEQLQAKYNLMFSLKILDFYRGNSIHL"
     gene            complement(2861..3976)
                     /locus_tag="PLI37_02405"
     CDS             complement(2861..3976)
                     /locus_tag="PLI37_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOQ57904.1"
                     /translation="MKKVIRIVLLVLLAAVVLWTFFFLWQKSRPQAVVYEIIEVEQRS
                     IEKKAVATGKVEPRNEIQIKPQIAGIIDEIYKEAGEMVRKEEVIARIKVIPDMVTLNA
                     AESRVKVAEVNLNQSQTDFDRQKGLYENKVISREEFEKSESGFILAKQEYQTAVDNLA
                     LTKEGITSKSSSQSNTLIRSTIDGMILNIPVKVGNSVIQSNNFNEGTTIATVADMSDM
                     LFVGKVDETEVGQIKENMPIKLTIGALNDRSFDAKLEYISPKGTEESGAILFEIKAAA
                     KITEDIFVRAGYSANAEIVLQSVTDVLSIPESSVEFSGDTSFVYVLTNEEPQQFEKRS
                     IEIGLSDGIYIEVKEGLDADSRLRGAAIDPKAKKPAL"
     gene            complement(4010..>5221)
                     /locus_tag="PLI37_02410"
     CDS             complement(4010..>5221)
                     /locus_tag="PLI37_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ57905.1"
                     /translation="KLRSLLTAFGVFWGILMLTLLLGVSNGLSNGMKSSIQGIATNSC
                     FFFSGTTSEPYAGFKKGRRWDIRLSDIDLIRSQVEGVRDVAPVVFGGRGTDNVMYGDK
                     LGSFNVRGNGPELVRIEEQNMLYGRYINEIDISQKRKVCVIGKNVFSAFFKEGTNPCG
                     LLIRCNGQYYRIVGVTKGVSNNISLGGRSDDMVVLPITTMQQLYNQGDVIHFFGVRAH
                     DHYDIAKLEENIKDILKSRHQISPTDPQAVVSINIGEMFKTFMYLFLGISILTWIVGV
                     GTLLAGAIGVSNIMLVSVRERTKEIGIRRALGAKPSNIISQIMTESLVLTALAGFVGF
                     TLGVGILALIDQIIANAPADSDIFLQQLMIDFKVGISAAAILLFFGLLAGLLPAMRAM
                     QIKPIEALSEE"
     assembly_gap    5222..5321
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5322..6388)
                     /locus_tag="PLI37_02415"
     CDS             complement(<5322..6388)
                     /locus_tag="PLI37_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ57906.1"
                     /translation="MKFFEFDTIREIFSTMRQSKLRTFLTGFSVAWGIFMLIVLLGSG
                     KGLQNGVSSNFSNYAVNMVNVWPRRTNMPYKGFKAYRRLRFRDQDMEMLKNHFPQIDK
                     ASGIISLYGSTVSYGKEYGNYGINGVMPDYKDIRMLSIEAGNGRFINHIDMSEERKVV
                     VINQRVADNLFREEEPLGKYIQIGGIAFMVVGLETKTSNDENGYCYIPHSTAQTIYNR
                     RGYTDEFYFTVTGMDSPEANEAFTLALRKTLARSLIVHPDDTAAIGVWNMAENYVQTM
                     RIFSTIQLFVFIIGLCTLVAGIVGVGNIMVITVKERTREFGIKKAIGASPSNILNSIL
                     IESVIITGVFGYIGMVLGIGTL"
     gene            complement(6398..7096)
                     /locus_tag="PLI37_02420"
     CDS             complement(6398..7096)
                     /locus_tag="PLI37_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ57907.1"
                     /translation="MILLEKLNKTYPGVQPLHVLKDIDLSISQGEYVAIMGASGSGKS
                     TLLNVLGILDDYDTGRYLLDGKLIAGLSEKKAAIYRNKMIGFVFQSFNLISFKNAMEN
                     VALPLFYKGVSRKKRNQLALEYLEKVGLRDWWHHYPNELSGGQKQRIAIARALISQPR
                     IILADEPTGALDSRTTDEVIQLLSQVNAEGITTIVVTHERSVAEQTKRIIHLKDGYIE
                     SDNINIPSLTACSK"
     gene            complement(7176..7640)
                     /locus_tag="PLI37_02425"
     CDS             complement(7176..7640)
                     /locus_tag="PLI37_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57908.1"
                     /translation="MKKTILALAVCLLGFTATFAQSKALGLRGGYGFEISYQQALGDA
                     NRLEADLGLGVFNGLNLAAVYQWVWSLESIGLPEGFKWYAGPGADLYFGFGNNEGNND
                     YGGISAGIGGQIGVEYTFPGIPLQLAIDCRPMWLFGNFGPYWGYAGAVRYVF"
     gene            7977..9350
                     /locus_tag="PLI37_02430"
     CDS             7977..9350
                     /locus_tag="PLI37_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOQ57909.1"
                     /translation="MTDVIKIKKGLDISLKGGAFPEVQSASYTENYALCPTDFHGLIP
                     RVLVKEGEQVLAGTPLVADKNKPEILFVSPLSGVVSAVKRGEKRRLLAIEIRADQKNA
                     ALSYPVRNPLDMDGVKVKEALLEAGLWPYIKRRPYDVIANPRVEPKAIFISAFDTAPL
                     APDYAFILKGQGHDFQTGLNALTKLTKGKVHLSIPAGSGSGELKNAQNVEIHRFEGPH
                     PAGNVGVQINRIDPVNKGETVWTVNALDVLFIGRFFNKGRVDFSRLVALTGPEVYEPR
                     YYRCVAGIQLSALIKGNVHKEIPLRYISGNPLTGTRVEMDDYLGAYDSQVTVLHEGTD
                     VHEFAGWVMPRLNMFSMSKTYFTGLVQKICPRKQFDADTRILGGERALIMSGEYEKVF
                     PMDILPEKLVRACITRDLEKMEELGIYEVAPEDFALCEYVCTSKVEVQRQVRAALDLL
                     KAENGEE"
     gene            9376..10578
                     /locus_tag="PLI37_02435"
     CDS             9376..10578
                     /locus_tag="PLI37_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOQ57910.1"
                     /translation="MKALRKLLDKIKPAFEKGGKLSMFHSVFDGFETFLFVPNRTSQS
                     GTHIHDSIDSKRAMFVVVIALIPALLFGMFNVGYQHYLAIGQAAGFFQTFFWGALAVL
                     PYIVVSYVVGLGIEFTIAQFRGEEIHEGFLVTGMLIPLIIPINTPLWMVALATAFAVI
                     FVKEIFGGTGYNIFNVALMARAFLFFAYPSKMSGDSVWVSLKDYFGFGQEQATIDVYT
                     GATPLGQAAIAGGDNLVLTGITGEPLSWWDTFLGLIPGSIGETSVLAILLGGILLIYT
                     GIASWKIILSVFAGGAFMAILLNIFAVNPYMEMPFWEHWCLGGFAFGAFFMATDPVTA
                     SRTEKGKWIYGFLIGLLAILYRVLNPAYPEGMMLAILFMNIFAKLVDYLVVQNNIKKR
                     LKRARIAQ"
     gene            10590..11321
                     /gene="nqrC"
                     /locus_tag="PLI37_02440"
     CDS             10590..11321
                     /gene="nqrC"
                     /locus_tag="PLI37_02440"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOQ57911.1"
                     /translation="MNKNSNTYIFIYASVMVILVAAALALTNGLLKDQHDKNSEIDKM
                     TQILRSIKVEADKNNAESLFAQNIQTAYILNSAGQVNNQSSPEAAFAVEMSGEVTKPL
                     EKRQLPVYEALVEGDTLYILPVYGAGLWGPIWGYVSLKNDRNTIYAASFSHSGETPGL
                     GAEISTEPFQAPFSGKVLYKEGEFRSIAILKSGQKADNQDAVDAISGGTITSKGVEDM
                     LHNCLGAYKAFFEQVETENTENKEE"
     gene            11325..11945
                     /locus_tag="PLI37_02445"
     CDS             11325..11945
                     /locus_tag="PLI37_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOQ57912.1"
                     /translation="MSSSRKQILINPISKNNPVVVLMLGICSALAVTAQVKPALVMGV
                     SVTAVLAFSNVIISLLRNTIPNRIRIIVQLVVVAALVIIVEQILKAYAYEVWGQLSVF
                     IGLIITNCILMGRLEAFALGNTPFDSFLDGIGNGLGYTAILLIVAIARELLGSGSLMG
                     YAVIPQSFYEGGYMNNGLMILPPMALIVVGCIIWVHRSIDKDLQEE"
     gene            11978..12592
                     /gene="nqrE"
                     /locus_tag="PLI37_02450"
     CDS             11978..12592
                     /gene="nqrE"
                     /locus_tag="PLI37_02450"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOQ57913.1"
                     /translation="MEAFNIFIKSIFVDNMIFAYFLGMCSYLAVSKTVKTSVGLGIAV
                     TFVLVVTLPINYLLDTYVLRPGALAWVSPELESIDLSFLSFILFIAIIASFTQIVEMV
                     VEKFTPALYSQLGIFLPLIAVNCAILGGSLFMQQRNFANIGQATAFGLGSGIGWLLAI
                     VGIAAIREHMSYSKVPKPLRGLGISFIVTGLMGIAFMGFMGIKF"
     gene            12605..13864
                     /gene="nqrF"
                     /locus_tag="PLI37_02455"
     CDS             12605..13864
                     /gene="nqrF"
                     /locus_tag="PLI37_02455"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOQ57914.1"
                     /translation="MILLAFNALTLVLGVVLFLVVILILVGLLIYVKDKLTPSGEVDI
                     DINEGEKILRTEMGDSLLSTLSNNQVFLPSACGGGGTCGMCKCRVLEGGGSILPTETG
                     FFSRREQQEQWRLACQVKVKDNLKIQIPEEILGIKKWECEVVSNHNVASFIKEFVVKL
                     PEGEQLKFKSGSYIQIDVPKIEVDFKDMNVDDRFKSEWDKFKLWGLHMSNPEPTYRAY
                     SMANHPAEGNIIMLNIRIATPPFDKQKGDWMKVNPGVCSSYIFSLKPGDKITISGPFG
                     DFHILDTDREMLYIGGGAGMAPLRSHLMHLFHTLKTTDRKISYWYGARSKNEIFYEED
                     FRAIEKEFPNFSFHIALSEPRPEDNWTGYTGFIHQVILDNYLINHEAPEDIEYYMCGP
                     GPMANAVKVMLDNLGVPKEMLMFDDFG"
     gene            14094..14945
                     /gene="hisG"
                     /locus_tag="PLI37_02460"
     CDS             14094..14945
                     /gene="hisG"
                     /locus_tag="PLI37_02460"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOQ57915.1"
                     /translation="MLRIAIQAKGRLYEETMALLTEANIKVDASRRTLLLPARNYPVE
                     ILFLRDDDIPQAVAGGVADAGIVGENEYVERREEVQIVQKLGFSRCRLSLAIPRDMDY
                     PGLSWFQGKKIATSYPGILKDYLQANKIESEIHVISGSVEVAPGIGLADAIFDIVSSG
                     GTLVSNRLKEVEVVMQSEALLICNPRLCEEKRALLDDLAFRFNAVRQAEGKKYIILNI
                     QNSKIPDVCRLLPGMKAPTVTPLTEEGWSSVHSVIEEKRFWEIIGKLKAIGAEGILVV
                     PIEKMIL"
     gene            14954..16234
                     /gene="hisD"
                     /locus_tag="PLI37_02465"
     CDS             14954..16234
                     /gene="hisD"
                     /locus_tag="PLI37_02465"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOQ57916.1"
                     /translation="MQVINYPPESAWPELMQRPAGNDTALLEQVSGILAQIKLKGDAA
                     VREYSLRFDGYAADSLELDAEEINSSCKEVDPKLKEAIQQAKRNIEAFHQAQVQAPIS
                     LEVQAGVKCWQKAQPIDKVGLYIPGGTAPLFSTVLMLAVPAQLAGCGEIVLCTPPGKE
                     GKIHPAILYTAHLCGIRRIFKIGGVQAIGAMAYGTETVPQVYKIFGPGNRYVTAAKQL
                     VSLEDTAIDMPAGPSEVLVMADRTAIPAFVAADLLSQAEHGSDSQVILLTSSAALAEA
                     VQKEIQTQCAALPRATIAWKALENSKIILLKDREEMLRFSNAYAPEHLIISTKDYRRL
                     ADRVTNAGSVFLGNYSPESAGDYASGTNHTLPTRAYARAYSGLNLDSFKKKISFQEIS
                     REGLQALGPTIEIMAENEELLAHKRAVSIRLKQD"
     gene            16253..17299
                     /gene="hisC"
                     /locus_tag="PLI37_02470"
     CDS             16253..17299
                     /gene="hisC"
                     /locus_tag="PLI37_02470"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOQ57917.1"
                     /translation="MLPLEQLLRKNIQELTPYSCARDEFQGQASVYLDANENPYNGPY
                     NRYPDPLQRELKQKIAAIKGLRPGQVFLGNGSDESIDVLFRAFCEPRIDNVVAIEPTY
                     GMYRVSAGINDVAYRRVLLDELFQIDAGRILAACDARTKLVFLCSPNNPTGNSLNRAE
                     ILKLLDRFEGILVLDEAYIDFASEPSLMGRLDDYPGLVVLQTFSKAWGSAAIRLGMAL
                     ASESIVKVLNKIKYPYNVNLLTQQQAAKMLESAPRVKDWVAQILQERERLRKALSELR
                     LVKHIYPSDVNFLLVRVSDANRLYTALTEQGIIVRNRNNVSLCLGCLRITIGTAGENQ
                     ILLQALKALDQNFS"
     gene            17329..18450
                     /gene="hisB"
                     /locus_tag="PLI37_02475"
     CDS             17329..18450
                     /gene="hisB"
                     /locus_tag="PLI37_02475"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HOQ57918.1"
                     /translation="MKKRALFIDRDGTLIEEPKPSLQVDNLDQLVFLPKVIRNLYFIR
                     KKLDFEFIMVSNQDGLGTDAYPTENFERVQNKMLQILQNEGICFDAIHIDPSLPEQNS
                     PGRKPGTAMLTSYMDGSYDLAASFVIGDRMTDVQLAHNLGCKCILLNEEINLQESKEY
                     AASCVMQSTDWDKISAFLFAGQRKVSIERKTKETEVKLELDLDGSGKTNIDTGIKFFD
                     HMLDQIGRHSGCDLSIIVKGDLEVDEHHTVEDTALVLGEALRQALGDKRGIERYGFCL
                     PMDDSLCSLALDFGGRPWLVWDVNFRREKLGELPTELIFHFFKSLSDSAKMNLNIKAL
                     GDNEHHKSESIFKAFALALKMAIHRDIYRYDLPSTKGLL"
     gene            18522..>18976
                     /locus_tag="PLI37_02480"
     CDS             18522..>18976
                     /locus_tag="PLI37_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ57919.1"
                     /translation="MKRIFFSLGLLTLVACPLFAQTSNLGLAADQPQEQPSRFPTELE
                     VSLSPKDIVYATKDTSVLKMDIYRPQAQDKALPCLVFLYGGGFSSGSKDNKTTVEFCH
                     QMTDRGYVVVAINYRHGMAGVKNKGFKMVRSLERAIGMAVEDLYSATDF"
     assembly_gap    18977..19076
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19256..>20404)
                     /locus_tag="PLI37_02485"
     CDS             complement(19256..>20404)
                     /locus_tag="PLI37_02485"
                     /inference="COORDINATES: protein motif:HMM:NF037079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOQ57920.1"
                     /translation="FSYNHFWDAGKASLCGMTGESGENWISYHHNWFDHSDSRHPRVR
                     VMSVHVYNNFYDGISKYGVGATMGASVFVEANYFRNTKRPMMISLQGTDATGDGTFSG
                     ENGGMIKSFGNVFAETPNLQFIPHTQSATSFDAYEAASRQDSVPSSYKSLVGSHTYNN
                     FDINEALMYTYSPHPAEEVPEIVCGPLGAGRMGKGDFTWSFDNAVDDASYAVNEALKA
                     AIASYSSSLIGFFGQAGNGDPGSGETGGGETGDDPGGGGDPAEEPGNGDIVCTFSRSA
                     GPSNDFFTVTGNYSNSKGTVNYKDSVYTECLKMESSTRIAFVLTDSRRLTLVFGPNEA
                     ASMKLNGIAYTDEDNDKIIVVDRLEAGSYEITKSGVCNLFYIALSPLE"
     assembly_gap    20405..20504
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20505..21382)
                     /locus_tag="PLI37_02490"
     CDS             complement(<20505..21382)
                     /locus_tag="PLI37_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57921.1"
                     /translation="MKKMLTLALLVVVLTGLQANTKLRVDLRDTDSVEHFSLEELESI
                     VFGENNEFSIYFKQGGSLHYPGTASGIQFVQEPDSTPNTIAIVATEGWLEAASVSWEP
                     LESVLSYRVYCKSQETGSEYRPLDSPLIRNYGSYGRADAIGLKAGYYRFKIVPLDREG
                     LEMDDFAAESDFVEVSAHDRSGFAHHNLNEGVGAYKNDGSLKDNAVVFYVTKDNCNSI
                     THNVIIDSKGKTETRTGIGKILQGKQKGHDSTPWAVRFIGTIRTEDFEAGQLLSDQDG
                     LLLKSNAGDAALNVTL"
     gene            complement(21394..21843)
                     /locus_tag="PLI37_02495"
     CDS             complement(21394..21843)
                     /locus_tag="PLI37_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ57922.1"
                     /translation="MKFRSILLPTLFCLFSLNIYSERIVRIEGQELQQALNRISFKGD
                     TAVLHFPGGDSLSIHCLLLSIEFTEGSGLSPLQNTRVLLARNPVKDILPLENLETGSR
                     LLLYNIEGRLVKTQQAHASSVQIDLSDLPAGVYLLRTGKYLVRFVKL"
     gene            complement(21854..23137)
                     /locus_tag="PLI37_02500"
     CDS             complement(21854..23137)
                     /locus_tag="PLI37_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOQ57923.1"
                     /translation="MKTTAVRLYGKKDLRLETFELPAIKDDEILAKIVCDSLCMSSYK
                     AAIQGADHKRVPDDVDKNPIIIGHEFAGELIEVGSKWAHKFKAGEKFSIQPAMNYEGG
                     PVGILSAPGYSYAYIGGDATYVIIPNEVMEQNCLLSYKGAGFYPASLSEPLSCVIGAM
                     HANYHTTPGSYVHKMEIVDKGKMAILAGVGPMGLAAINYVLHREDRKPSLLVVTDIDQ
                     ARLDRAARLYTPEFAASRGIKLVYVNTAALPDPVAGLKELSSGEGYDDVFVFAPVRSV
                     IEQADAILAFDGCLNFFAGPSDPKFSAMMNFYNVHYAYTHVVGTSGGNTDDMIEALEL
                     MGRGMDPAGLITHIGGLNAVIEATLNLPSIPGGKKLIYNHIDLPLTPISDFARLGESN
                     PVFRKLAEICEKNRGLWSIEAENYLLENCASHPKK"
     gene            23350..24873
                     /gene="purH"
                     /locus_tag="PLI37_02505"
     CDS             23350..24873
                     /gene="purH"
                     /locus_tag="PLI37_02505"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOQ57924.1"
                     /translation="MSDNKSIRTALISVYHKEGLDEILHQLHREGVEFISTGGTQQFI
                     ESLGYPCEKVESLTGYPSVLGGRVKTLHPKIFGGILNRRSLDTDRQECVEYDIPAIDL
                     VIVDLYPFQETVKSGAAAEDIIEKIDIGGISLIRAAAKNFNDVVIVASKAQYPALLDL
                     LNEQGATTSLEDRRWFAREAFAVSSGYDAAIFNWFDGADGSALRVAIDHSKVLRYGEN
                     PHQQASFFGDFDELFEQLNGKEISYNNLLDIDAAVSMVNDFDETTFVIVKHNNACGLA
                     SRPVLVDAWNAALSADPVSAFGGVFATNAAIDKTTAEEINKLFFEVLIAPDYDFDALE
                     ILTQKKNRILLIQKEIPQGGKQFRSLFNGVLVQDKDTHTETAEDLRCVTEKESDAAQI
                     EDLLFANKVVKHNKSNAIVLVKDKQLCAAGVGQTSRVDALRQAIDKARSFDMELEGAV
                     MASDAFFPFPDCVEIADEAGITAVIHPGGSINDKLSIEYCNEHGLAMYTTGYRHFKH"
     gene            24881..25900
                     /locus_tag="PLI37_02510"
     CDS             24881..25900
                     /locus_tag="PLI37_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOQ57925.1"
                     /translation="MGIFSFTQKIAIDLGTANTIIMCNDKIVVDEPSVVALDRKTDKM
                     LAVGEKARLMHEKTHENIRTVRPLRDGVIADFYAAEQMIRGLIKIALPRGSGFFSSLR
                     MVVCIPSGSTEVELRAVRDSAEHVGGREVYMIYEPMAAAIGIGIDVEAPEGNMIVDIG
                     GGTTEIAIISLGGIVANKSTRIAGDDFTTDIQEYMRRQHNLRIGERTAERIKINVGSA
                     LTELENPPEDFLVYGPNQMTALPMEVPVSYVEIAHCLEKSVSKVETAILNALEQTPPE
                     LYADIVRNGIYLTGGGSLLRGLAKRLSDKINIPFIVPEDPLHAVARGTSVTLKNVDRF
                     NFLMR"
     gene            25900..26727
                     /gene="mreC"
                     /locus_tag="PLI37_02515"
     CDS             25900..26727
                     /gene="mreC"
                     /locus_tag="PLI37_02515"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOQ57926.1"
                     /translation="MRQLIHFLVRYRNFFLFLFLEILSLILLFRFNDYQQGVLFSSAN
                     RINGKLYTFREAVSGFFALRENNTELLRINSEMELKIARLEESLRKAGLEEEQLKAAG
                     SYEFIHARVIKNSVSQLRNYLTLNVGKLDGVAEGMGVCNHLGIVGVVSVCSDHFSVVI
                     PLLNPDISFSCIVSGSNSVGSLNWKGGDTRFAWLEELPDYAFWAIGDTVVTSGFSDAF
                     PYGIPVGVIESIERKSNSSFHRSKVRLFTTFESLADVRVISHFLREEKLELEKELKQ"
     gene            26724..27212
                     /gene="mreD"
                     /locus_tag="PLI37_02520"
     CDS             26724..27212
                     /gene="mreD"
                     /locus_tag="PLI37_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOQ57927.1"
                     /translation="MIRLFLKHLFLFVLLFLLQIIVFQQLYFWYVTPLVYVYILIRWP
                     ATLPAGWVLILGFLLGLLVDINVDTPGMHAAATTFVAFLRPSLLKGFLNRDEVENLEP
                     REKSMGFLPYWKYAVSIVLLHHTALYVLESFSFFDPLLLLVKIVGSALLSLLLIFLFE
                     RF"
     gene            27232..>27704
                     /locus_tag="PLI37_02525"
     CDS             27232..>27704
                     /locus_tag="PLI37_02525"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOQ57928.1"
                     /translation="MRSSTTDYENRGRIFILIIVGVIFIFIVRLAYLQLFSEEYDKYA
                     DSNALFKKTIYPERGVIYDRNGKLMVYNQAFYDIMVTPREMQGLDTLEFCSTLKIDRA
                     FFEKRMTDIRNRRLNPGYSSYTPQLFMSQLSVEAYGPLQEKLYRFPGFSITNRSL"
BASE COUNT         6971 a         6276 c         6639 g         7418 t
ORIGIN      
        1 ggctgaatta tgtagtaacg gccagagcac gcaatcgcat taagcaaacc ctgaaggaac
       61 aaacccagaa acaggctgag tacgggaggg aaattttaca gcggcgcatg aagaatcgca
      121 aactggaatt tgacgagggg gtgatgacca aagtggtgcg aaaatttaaa tacaaaaccc
      181 tgaccgattt tcacgctgct ttaggaacgg agcaactcga cctgaatcaa gtgattgatc
      241 aatacgtgga atggtatcag cacgacaaaa ttgctcacga agctccacag gttaccacag
      301 ccgaaaaata cgtcattgaa cccacgcctc agcaaataca gcaggcaaaa gaagatattc
      361 tggtgattga tcaaaatctg aaaggcattg attttacctt gtcaaaatgc tgccatccca
      421 tatacggaga cgaagtcttt ggttttgttt ctgttacggg aggaattaaa attcaccggg
      481 aggattgccc caacgccccc caaatgaaaa cccgctttcc gtaccgtatt gtaaaagccc
      541 gttgggcagg aaaatcggga gtcggacaat acgctgcctc gctcgaggtg atagggaaag
      601 acgatatcgg cattgtgacc aacatcagtt cccttatttc caaagagagc gacctgggcc
      661 ttcgttccat ccgaatcgat tccaacgacg gcctgttcag gggttatttg acagtggtag
      721 tacaagatgc ccatcagttg gaagcgctga tcaaaaaaat aaaaaccatt aagggagtta
      781 aacaagtaga aaggggagcc tgataaacca tgaaccccga aagttgaata aaaaactttc
      841 ggggttcacg tcaattttta ttgttttctg tgtagatccg tgggttagaa cagaatggcc
      901 aggctgatgc tcaggttgcg gtttttcagc gagctgaaca gaggattcac gtctttcaga
      961 actgtaccgg ccacttcctt gaattcgctc agaccaaagt cgtattttac cgctaattgc
     1021 agaaagagaa cttcgacacc ggcaccaaat ccgatcccgt agtcaaagga attcaaaggc
     1081 tttccgtcgt aattactcag gaagtctttg taagcgtcgt agaagtcttc ggctccgtct
     1141 attttgtcgg agatcaggta ggagagatag gggctcactt gtccgtaaac acggaacaga
     1201 ctgcccaaac ctactccggc ctggatgttt aggggtactt ccannnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnaccctga aacaggatag ccatcgttaa aataatagct
     1381 gtttttttca ttggaatgaa tttttgggta aatgtgaatg cggcaaaact acagcattta
     1441 tactatcaaa acaataattc cccggattat ttttaaacat aattcataat ccggttttgt
     1501 ttttcagact ttttttcgaa agaaatccgg ttacattata aatgaatgga attccctctg
     1561 tagaaatcca gtatttttaa actgaacatc agattgtatt tagcctgcag ttgttcggaa
     1621 cgggttctga gcagttgcat cctggcttcg ttgtattcgt aaatagtaga acgtccgctg
     1681 ttgaattttt cctcggtaaa acggaaggct tcctgtgtgg cgcgtatggt ttcgaacgaa
     1741 gcccggtatt tttcttcggc cgtattggca ttgaaccagg cttgctgaat ttctttatag
     1801 agtatttttt ttgtgttttc cagctgcaat ctggctgttt ccagattgat gttggagcgt
     1861 ttaatattgt tggtagtgtt caggcggtta aaaataggga tgctgagact cagtccgata
     1921 ttgcttcgcc cgttggtgct gagctgatct ttgaagctgg ggttggcata attgctgtgg
     1981 tcgtaataac tgttggagta ggaagccccc aagctgaggc tggggtagcg ggctccccgc
     2041 gatacttcaa gcatcttttc ggaagtcatc acctcgtatt gagccgattt aatggcggct
     2101 ctgttgcttt gcgcctgatc gtaaatgctc tcggcaggca actgaagggc ttccttgctc
     2161 accatgattt gtgaatcggg ttcgctgatg ttgaagtctt tccagtcttc caattccagc
     2221 aactgactca attccaacaa agaaagctcc attgtttgtt tctgttgcgt ggccgacagt
     2281 tcgtcctgag ccagtttggc cttgatttcg tagagctggc cttcgggaat tttatcgtgg
     2341 gcgtagcgaa tttcgtattg ccggagttgt tccttgctca aatcgacctg ttccagggcc
     2401 aattgataca attccttatt gtaaagaatt tgcaggtaag ccgaggcaat attcaacgac
     2461 aggtcttctt tggctttgtt caggttttcc agcgaagcca gcgtgctgta ttgttgcgct
     2521 ttgacgctgt tgttgatttg aaaaccgttg aaaagagtga ttccggtaga gagtccggcc
     2581 gaagtcgact gtgaattgct gtttacgtaa acaccggtat tgtcctgggt ttgtccgaaa
     2641 ccgatgttat gcgaggcaga ggccgaaaca tagggcaaac gtcggtattg cgtgtttttt
     2701 ttctcaattc cctgtatttc cgcgttcagg ccggccgttt tgatggcaat gttattagcc
     2761 agagcatagt cgatgcattc tttcagggtc cacacttttt gagcttgcag gctgaaagcg
     2821 gcaagcagag ctgtgctcag caggctgatt actgtttttt tcatagggca ggttttttgg
     2881 ctttgggatc gatggcggct cctctcagcc ggctgtcggc atccaagcct tctttgactt
     2941 cgatgtaaat gccgtcggaa aggccgattt ctatggatcg tttttcgaat tgctggggtt
     3001 cctcgttggt cagtacgtaa acaaaagagg tgtcaccgct gaattccaca ctgctttcgg
     3061 ggatggacag aacgtcggta acgctttgaa gcacaatttc cgcgttggcg ctgtaaccgg
     3121 cacgcacaaa aatgtcttcg gtgattttgg ccgctgcctt gatttcgaaa aggatggctc
     3181 cactttcttc tgtacctttg ggtgaaatgt attcgagctt ggcatcaaaa ctacggtcgt
     3241 tcagggcccc gatggtgagt ttaataggca tgttttcttt gatttgaccc acttcggttt
     3301 cgtctacttt gccgacaaaa agcatatcgc tcatgtcggc caccgtggca atagtggtgc
     3361 cttcgttgaa attgttggac tggataaccg aattgcccac tttgaccgga atattcagaa
     3421 tcataccgtc gatggtggaa cgaatcaggg tgttgctctg gctactcgat ttgctggtga
     3481 tgccctcttt ggtcagggcc aggttgtcga cggctgtctg gtattcttgt ttggccagaa
     3541 taaaaccgga ctcacttttt tcgaactctt ctctggaaat cactttgttt tcgtacaggc
     3601 ctttttgacg atcgaaatcg gtttgcgact ggttcagatt gacttcggcc actttgaccc
     3661 tcgattcggc tgcgttgagt gtaaccatat cggggattac tttaatgcgg gcaatgactt
     3721 cctcttttcg taccatttcg ccggcttctt tgtaaatctc gtcgataatg ccggcaattt
     3781 ggggtttgat ctgaatttcg ttgcgaggtt ctaccttgcc tgtcgcgacg gctttttttt
     3841 cgatgcttct ttgttctacc tcgatgattt cgtaaactac cgcttgagga cgtgatttct
     3901 gccagagaaa aaagaaagtc caaagcacga ctgcagctag gaggacaagc agaacgattc
     3961 taataacttt tttcataaag gaggtttttt tgatatgatt attatttgtt tattcttcgc
     4021 ttaaagcttc tatgggttta atttgcatgg ctctcatggc ggggagcagg ccggccagta
     4081 atccgaaaaa caggagaatg gctgcagctg aaattcccac tttgaaatcg atcatcaatt
     4141 gttgtaaaaa gatgtcgcta tcggccggtg cgttggcaat gatctgatcg atcagggcaa
     4201 gtatgcccac gcccagcgtg aagcctacaa atccggccag ggcagttaat accaggctct
     4261 ctgtcattat ttgtgaaatg atgttggagg gttttgctcc cagggcccgg cgaataccta
     4321 tttctttggt gcgctcgcgg accgatacca gcataatgtt gctgacgcct atggctccgg
     4381 ccagcaaagt gcctacgcca actatccagg tcaaaatgga aatccccaga aacaaataca
     4441 tgaatgtttt aaacatttcg ccaatgttta tggatacaac cgcctgtgga tcggtgggtg
     4501 aaatctgatg acggcttttg agaatgtctt tgatgttttc ttccagcttg gcgatgtcgt
     4561 aatgatcgtg ggcacgtacc ccgaaaaagt gaattacatc gccctggtta tacagttgtt
     4621 gcatagtggt gatgggcaaa accaccatat cgtcgctgcg tccccccaga gaaatattgt
     4681 tcgatacgcc tttggtgaca cccactatcc ggtaatactg tccgttgcat ctgatcaaga
     4741 gcccgcaggg attggtgcct tctttaaaaa aagcggaaaa gacattcttg ccgataacgc
     4801 aaaccttacg cttttggctg atgtcgattt cgttgatgta cctgccatag agcatatttt
     4861 gctcttcaat gcgcaccagt tcaggtccgt ttcctctgac gttgaaacta cctagtttgt
     4921 ctccgtacat cacattgtcc gtgcctctgc ctccaaaaac gacaggggcc acatctctga
     4981 ctccttcaac ctgggaacgg ataaggtcaa tatccgaaag acgtatatcc cagcgccttc
     5041 cttttttaaa tccggcgtag ggttcggagg tggtgccgga aaagaaaaaa caggaattgg
     5101 tggcaatgcc ctgaatactg cttttcatgc cgttgctcag gccgttgctg acacccagta
     5161 acagcgttaa catcaaaata ccccagaata caccaaaggc cgtgagaaga ctcctaagct
     5221 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagtgttcct atccccagca
     5341 ccattcctat atagccgaag accccggtga ttatgaccga ttctatcaga atggaattca
     5401 atatattgga ggggctggct ccaatggctt tttttattcc aaactcgcgg gttctttcct
     5461 ttacggtaat aaccataata ttgcccactc cgactatgcc ggcaaccaag gtacataagc
     5521 cgattatgaa gacaaataat tgtatggtgg aaaaaatgcg catggtctgg acataatttt
     5581 ctgccatatt ccatactccg atggcagcgg tgtcgtcggg atgaactatc agcgacctgg
     5641 cgagggtttt gcgcaaggcc agggtaaagg cttcgttggc ttcggggctg tccatgccgg
     5701 taaccgtaaa ataaaattca tcggtatagc cccttctgtt gtaaatggtt tgagccgtgg
     5761 agtgggggat gtagcaatag ccgttttcgt cgttcgaggt tttggtttcc aggccaacca
     5821 ccataaaggc tatgcccccg atctggatgt attttcccag cggctcttct tccctgaaaa
     5881 gattatcggc aacccgttgg ttgattacca ccaccttgcg ttcttccgac atatctatat
     5941 ggttgataaa gcgaccgttg ccggcttcta tgctgagcat cctgatgtct ttgtagtcgg
     6001 gcattacccc gttgattccg taattgccgt attccttgcc gtagctgacg gtactgccgt
     6061 aaagggaaat tatccccgaa gccttatcga tctgaggaaa atgatttttg agcatttcca
     6121 tgtcctgatc cctaaagcgc aaccggcggt aagctttgaa gcctttgtag ggcatattag
     6181 tgcgccttgg ccagacattc accatgttga ctgcgtagtt gctaaagttg gaacttacgc
     6241 cgttctgcaa acctttgccc gagccaagca gcacgataag cataaaaata ccccaagcta
     6301 ccgagaaacc tgtcaaaaag gttctcagtt tgctctgccg catggtgctg aaaatttctc
     6361 taatggtgtc gaactcgaaa aatttcataa aacaggctta tttgctgcag gctgtcaggc
     6421 tgggaatatt gatattatcg gattcaatgt agccatcttt cagatgaatg atgcgtttgg
     6481 tctgttctgc aaccgacctt tcgtgagtta ccacaatcgt ggtgattcct tcggcattta
     6541 cctgtgagag gagttgtata acttcgtcgg tagtacggct gtctagggca ccggtaggtt
     6601 cgtcggccaa tataatacgg ggctgggaga tcaaagcccg ggcaatggca atgcgttgtt
     6661 tctgaccgcc cgacaattca ttggggtagt gatgccacca atctctcagg cctacttttt
     6721 cgaggtactc caaagccagt tggttgcgtt tcttgcggga gacgcccttg taaaacaagg
     6781 gcaatgccac attctccatg gcatttttaa aggagatcag gttgaaagac tgaaacacaa
     6841 agcctatcat tttgttgcgg tatatcgccg ctttcttttc actcaatccg gcaattaatt
     6901 taccgtccag taaatacctg cccgtatcgt aatcgtccaa tatgcccaat acatttaata
     6961 gggttgattt tcctgaacct gaggccccca tgatagctac gtactctcct tgacttatgc
     7021 taaggtcaat gtctttcaac acatgtagag gctgtactcc gggataggtt ttgttgagtt
     7081 tttctaagag gatcattccg ccgatagact tacttaaata aaacggcagt tgcccttcaa
     7141 taggcaactg ccgcataaaa atcgtttatc cattattaaa agacataacg gactgctccg
     7201 gcatagcccc agtaaggacc gaaattgcca aacagccaca tgggacggca gtcgatggcg
     7261 agctgaaggg gaataccggg aaatgtatat tccacaccaa tttgtccgcc aatacctgct
     7321 gaaatacccc cataatcgtt gtttccttca ttgtttccga atccaaagta gagatcggct
     7381 ccgggtcctg cataccattt aaaaccttcg ggaagtccta ttgattccag gctccaaacc
     7441 cactgataca cagcagcaag gttcagtccg ttgaaaactc ccaggccaag gtcagcttcc
     7501 aaacggttgg catcgcctaa agcttgttgg tatgagattt caaatccata accgccgcga
     7561 agaccaagag ccttgctttg tgcaaaagta gctgtaaaac ccaaaagaca aacggctaat
     7621 gcaagaatag tttttttcat aactaaattc agtattgagt gagactagta aaataccctt
     7681 tttcctctaa tgaaaaagcg gcacaaaagt aggcaagaaa acttgcattg acatatacag
     7741 atacgttaac aagtatgaat attaatgttt tttcttttta aatctctggt attcagttta
     7801 taaaattatc agcaaaatta aatacagaaa aatttattta tttgcacgaa tttatgcgaa
     7861 ggttggaaaa atagttgtaa tttcgcggcc gaatttggtc cgctttgaaa ataatgatca
     7921 ttttcaggct taaaactttt ctgaaaacag tgggtgatcc gactaaaaat aacactatga
     7981 cagatgtaat taaaattaag aaaggcttgg atatcagtct taaaggtgga gcttttccgg
     8041 aagttcagtc tgcgtcgtat acggaaaatt atgcgctctg tccgactgat tttcacggac
     8101 tgatacccag ggttctggtt aaggagggag agcaggtttt ggccggaact ccccttgtgg
     8161 cagacaaaaa caagcctgag attctttttg tcagtcccct gagtggtgtg gtaagtgcag
     8221 tcaaaagggg tgaaaaacgc agattgctgg ccatcgaaat ccgtgccgac caaaaaaacg
     8281 ctgccttgag ttatcctgtg cgcaatcctt tggatatgga cggagttaaa gtgaaagaag
     8341 ctcttttgga agccggcctt tggccctata ttaaaagaag gccttacgat gtgattgcca
     8401 atccccgtgt agagcccaaa gctattttta taagtgcttt tgataccgct cctctggcgc
     8461 ccgactatgc ttttattctc aaaggccagg gccatgattt tcagacaggg ttgaatgccc
     8521 tgaccaaatt gaccaagggt aaagtacatt taagcatacc cgccggttcc ggttcgggcg
     8581 aactcaagaa cgctcagaac gtcgaaattc accgcttcga aggccctcat cctgcaggca
     8641 acgtcggtgt gcagatcaac cgcatcgatc cggtaaataa aggagaaacg gtctggaccg
     8701 taaatgcttt ggatgtgctc tttattggcc ggttctttaa taagggcaga gtcgattttt
     8761 cgcgtttggt agcactgacc ggccccgaag tatacgagcc ccgttattac cgttgtgttg
     8821 caggtattca gctctcggcc ctgatcaagg gcaatgtaca taaagaaatt cctctgaggt
     8881 atatcagcgg caatcccctc acaggcacga gggtagaaat ggacgattat ttgggcgcct
     8941 acgacagtca ggtcaccgtg cttcacgaag gtacggatgt acacgaattt gccggctggg
     9001 taatgccccg tttgaatatg ttcagcatga gcaagaccta tttcaccgga ttggtgcaaa
     9061 agatttgccc cagaaagcaa ttcgatgccg acaccagaat actgggcgga gaaagagctc
     9121 ttattatgtc cggcgaatac gaaaaagtgt ttcctatgga tattttaccc gagaaactgg
     9181 tgcgtgcctg cattacccgg gatttggaaa aaatggagga attgggcata tacgaagttg
     9241 cccccgaaga ttttgctttg tgcgaatatg tttgtacttc taaggtggaa gttcaaagac
     9301 aagtccgggc agctctggat ttgttgaaag ccgaaaacgg agaagaatag tagtcattac
     9361 aaaaaacacc atactttgaa agctttgcga aagttacttg acaagatcaa gcccgccttt
     9421 gagaaaggtg gaaaattatc gatgtttcac tcggtttttg acggttttga aacttttttg
     9481 tttgttccca atcgtacttc ccaatcggga actcatattc acgacagcat cgattccaaa
     9541 cgagccatgt ttgtcgtggt tattgctctg atccctgctt tgcttttcgg gatgttcaat
     9601 gttggttacc agcattatct ggccattggc caagctgccg gtttcttcca gacttttttc
     9661 tggggagccc tggcggtgtt accttatatt gttgtttcgt atgtggtagg attgggtatt
     9721 gagtttacca ttgcccagtt caggggcgaa gaaattcacg agggattttt ggtaaccggt
     9781 atgttgatac cccttatcat tccaatcaac acgcctttat ggatggtggc tctggcgacg
     9841 gcttttgccg taatattcgt caaagaaata tttggaggaa ccggctataa tattttcaat
     9901 gtcgccctga tggcaagagc ctttttgttt tttgcctatc cttccaagat gtcgggcgac
     9961 tccgtatggg tcagtctgaa agattacttc ggttttggac aggaacaagc aacgatagat
    10021 gtttatacgg gagcaacacc tctgggacag gcagccatcg ccggcggcga taatctggtc
    10081 ctgacaggca taacaggcga accgctgagc tggtgggata cctttctggg cctgattccg
    10141 ggctccatag gagaaacttc tgttttggcc atacttttag gcggaatatt attgatatac
    10201 acaggcattg ccagttggaa gattattctt tcggtttttg ccggcggcgc ttttatggct
    10261 atcctgctca atatttttgc ggtaaatccc tacatggaaa tgcccttctg ggaacattgg
    10321 tgcctgggcg gcttcgcttt cggagccttt tttatggcca ccgaccctgt gactgcatcc
    10381 cgtaccgaaa agggtaaatg gatttacggt ttccttatcg gcctgttagc catactctat
    10441 cgtgtgttga atccggctta ccccgaaggt atgatgctgg ctatactgtt tatgaatatt
    10501 tttgccaaac tcgtcgacta tctggtcgtg cagaacaaca tcaaaaaacg gctcaaaagg
    10561 gcgcgcatcg ctcaataata tttaggacta tgaataaaaa cagcaatacc tatattttta
    10621 tctatgcttc ggtaatggtt attctggtcg ccgccgccct ggccctgacc aacggactgc
    10681 tcaaagacca gcacgataaa aacagtgaga tcgacaaaat gacgcagata ttgcgctcca
    10741 taaaagtgga agccgataaa aacaatgccg aaagtctttt tgcacagaac atacaaacag
    10801 cctacatact gaacagcgcc ggccaggtga ataaccaatc atcgcccgaa gctgcttttg
    10861 ccgttgaaat gtccggcgaa gtaaccaagc ctttggaaaa acgtcagctc ccggtctatg
    10921 aagctctggt agaaggagac actctctata ttctgccggt gtacggagca ggcctttggg
    10981 gaccgatttg gggttatgtc tctttgaaaa acgacagaaa taccatttat gctgcctctt
    11041 tctctcactc gggagaaacg cccggattgg gggcagagat aagcactgag cctttccagg
    11101 cgcctttttc gggcaaggtg ttgtataaag agggcgaatt tcgttccata gctatactca
    11161 agtccggtca aaaagcggac aatcaggacg cggtagatgc tatttcagga gggaccatta
    11221 ccagcaaagg agtcgaagat atgttgcaca attgcctggg agcctataag gcattttttg
    11281 aacaagttga aacagagaac acagagaaca aggaggaata aactatgagt tcatcaagaa
    11341 aacagatttt aattaatcct atcagcaaaa acaatccggt tgtggtgctg atgctgggca
    11401 tttgttcggc tctggccgta acggctcagg taaaaccggc cctggtgatg ggtgtctcgg
    11461 ttacggcggt tttggccttt tccaatgtca ttatttctct attgcgcaac accattccca
    11521 atcgtatacg catcattgta caactggtag tggtcgctgc tctggttatc atagttgaac
    11581 agatattgaa ggcttacgct tacgaagttt gggggcaatt gtcggtcttt atcggactga
    11641 tcattaccaa ctgtattttg atgggacgcc tcgaagcttt tgctttgggt aatactccct
    11701 tcgattcttt cctcgatggt ataggaaatg gcctgggcta tacggccatt ttactgatag
    11761 tggccattgc tcgtgaattg ttgggttcag gctctctgat gggttacgcc gtgattccac
    11821 agtcctttta cgaagggggt tatatgaaca atggtttgat gattttaccg cccatggcct
    11881 tgatcgtagt gggttgtatt atctgggtac accgcagtat tgataaggat ttacaagaag
    11941 aataaaccta agggaatagt cttacaaata gaagaatatg gaagcattca atatttttat
    12001 caagtcgatt tttgtcgaca atatgatttt cgcctatttc ctgggcatgt gttcttatct
    12061 ggcagtatcc aaaaccgtta agacttccgt gggccttggt atagccgtta cttttgtgtt
    12121 ggttgttacc ttgcccatca attatttgct cgacacttat gtgttgagac cgggagcctt
    12181 agcttgggtc agtcctgagt tggaaagtat cgatctgagt tttcttagtt tcattttatt
    12241 tattgccatc attgcctctt ttacccagat tgtcgaaatg gtggtcgaaa aattcacgcc
    12301 tgctctgtat tcacagttgg gtatcttttt gcctctgata gcggtgaatt gcgccatact
    12361 gggaggttca ttgtttatgc aacaacgcaa ttttgctaac attggccagg ccacggcctt
    12421 tgggctgggc tcaggtatcg gctggctgct ggctatagtc ggcatagcag ccatacgcga
    12481 acacatgagc tattccaaag tacccaaacc gcttcgggga ctgggtattt cttttatcgt
    12541 caccggtttg atggggattg cgtttatggg atttatgggg attaaatttt aggaggaatt
    12601 ttagatgatt ttgttagcat ttaatgcctt gacacttgta cttggtgtag ttttattttt
    12661 agtagtaatc ctcatactgg tcgggctgtt gatttatgta aaagacaaac tgaccccctc
    12721 gggcgaagtg gacatcgata taaatgaggg tgaaaaaatc cttaggactg aaatgggcga
    12781 cagtttatta tcgaccctga gcaacaatca ggtcttcctc ccctcggctt gcggcggcgg
    12841 cggtacttgc ggaatgtgca aatgtcgtgt acttgaaggc ggcggcagca tattacccac
    12901 agaaaccggt tttttcagcc ggagagaaca acaggaacaa tggagactgg cttgccaggt
    12961 caaagtgaag gacaacctta aaattcagat tcccgaagaa attttgggca ttaagaaatg
    13021 ggaatgcgag gtggtttcca atcacaacgt ggcttccttt atcaaggagt ttgtagtgaa
    13081 attgcccgaa ggcgaacagc tcaaattcaa atccggtagc tatattcaaa tcgacgttcc
    13141 caaaattgag gttgacttca aagatatgaa tgtggacgac aggttcaagt ccgaatggga
    13201 caaattcaag ctttggggcc tgcatatgtc caatcccgaa cccacctatc gggcctattc
    13261 tatggccaat cacccggccg aaggcaatat catcatgctg aacatccgca ttgccacacc
    13321 tccgttcgac aaacaaaaag gcgattggat gaaagtgaat cccggtgtgt gttcttccta
    13381 cattttctcg ctaaaacccg gcgataagat cactatttca ggtcctttcg gtgattttca
    13441 catactggat accgatcgcg aaatgctcta cattggaggc ggtgccggta tggctccttt
    13501 gcgttcgcat ctaatgcatt tgttccatac actaaaaacc accgatcgta aaatttcgta
    13561 ctggtacggt gcccgttcca aaaacgaaat tttctacgaa gaggatttca gagccatcga
    13621 aaaggaattt cccaatttca gttttcatat agctttgagc gaacctcgtc ccgaagacaa
    13681 ttggacagga tacacgggct tcattcatca ggtgattttg gataattacc tgatcaacca
    13741 cgaagctccc gaagacattg aatactacat gtgcggtccc ggtcccatgg cgaatgccgt
    13801 taaagtgatg ctcgacaacc tgggagtacc caaagaaatg ttgatgttcg acgattttgg
    13861 atgattaaaa gcttattttt ttgtacaata acaatttatt gatacatttg cggcgtgcta
    13921 acaacacaga catggataaa ttgaacagat accacggaca tcatcatcat tccggacgtt
    13981 gaaaaacggt tggttgattg gttgtgtccg catcattcat aaaaggctta ccgttttgag
    14041 taagcctttt tttttgacaa agcaacattc gtttgacgag aaaaataaac aatatgctca
    14101 gaattgcaat ccaggccaaa ggccgcctgt acgaagaaac aatggccttg ttgacggaag
    14161 ccaacataaa agtggacgcc tcgaggcgca ctttgttgtt gcctgccaga aattatccgg
    14221 tcgaaatttt gtttttgcgc gacgacgaca ttccacaggc ggtagccggt ggagtagccg
    14281 atgccggtat agtaggggag aacgaatacg tcgaacgtcg cgaagaagtg cagatagtgc
    14341 agaagctggg ctttagccgc tgcaggctct cgctggccat tcccagagac atggattatc
    14401 cgggtctttc ctggtttcag ggtaagaaaa tagccacttc ctatccggga attctcaaag
    14461 attatttaca agccaacaag attgagtccg aaatacatgt tatcagcggt tcggtagaag
    14521 tggctcccgg catcggattg gccgatgcca tttttgacat cgtcagcagt ggcgggactt
    14581 tggtcagcaa ccggctcaaa gaagtggaag tggtcatgca gtctgaagcc ctattgattt
    14641 gtaatccccg tctctgtgag gaaaaaagag ccttgctcga cgatctggct ttccgtttca
    14701 acgccgtacg tcaggccgaa gggaaaaagt acatcatcct gaatattcag aacagcaaaa
    14761 ttccggacgt ctgccgcctg ttgccgggaa tgaaagcccc caccgtaacg ccgctgaccg
    14821 aggaaggctg gtcttcggtg cattccgtga tcgaagaaaa acgtttctgg gaaatcattg
    14881 gtaaactcaa agccattggc gcagaaggta ttttggtggt gcccatagaa aaaatgatcc
    14941 tataaatctt ctcatgcaag ttataaacta tcctcccgaa tcggcctggc ccgagttaat
    15001 gcaacgcccc gccggtaacg ataccgctct tcttgagcag gtgtcgggca tactcgcgca
    15061 aattaaactc aaaggagacg ccgccgtcag ggaatattcg ctccgattcg acggctatgc
    15121 agccgactca ttggagcttg atgccgaaga aataaactca tcgtgcaagg aggtcgatcc
    15181 taaattgaaa gaggccattc agcaggcgaa acgcaatatt gaagcctttc atcaagctca
    15241 ggtccaggcc cctatcagcc ttgaggtgca agccggcgtg aaatgttggc aaaaagcgca
    15301 gcccatcgat aaagtcggtt tgtacattcc cggcggaacc gctcctttgt tttcgaccgt
    15361 tttgatgctg gccgttcccg cccaattggc cggatgcgga gaaatagtgc tgtgtacgcc
    15421 tcccggtaaa gaggggaaaa tacatcccgc tattctttat acggctcatt tgtgcggtat
    15481 aaggcgaatc tttaaaatag gcggagtaca ggccattggg gctatggctt acggaacgga
    15541 aacagtgccc caagtctata agatttttgg tccgggcaat cgttatgtta cggcagccaa
    15601 gcaattggtc agtctcgaag acacggccat cgacatgccg gcagggcctt ctgaagtatt
    15661 ggttatggcc gaccggacag ccatacctgc ttttgtagcc gcagacttgc tttcgcaggc
    15721 cgagcacggc agcgacagcc aggttatctt gctgacctct tctgccgctt tggccgaagc
    15781 cgtacaaaaa gaaattcaga ctcagtgtgc cgctctgcca cgtgcaacta ttgcttggaa
    15841 agcattggaa aacagtaaga tcattctact gaaagatcgt gaagaaatgt tgcgtttcag
    15901 caacgcttac gcacccgaac acttgattat ttccacgaaa gattaccgcc ggctggccga
    15961 ccgggttacc aatgccggtt ccgttttctt gggcaactac agccccgaaa gtgccggcga
    16021 ttacgcttcg ggcaccaacc acacgcttcc caccagggct tatgcgcggg cctacagcgg
    16081 tttgaacctg gatagtttca agaaaaaaat cagttttcag gaaatcagcc gcgaaggttt
    16141 acaggcccta ggcccgacta tcgagatcat ggccgaaaac gaagagcttc tggcacataa
    16201 acgagccgtg agtatacgtc ttaaacaaga ttaattcaaa cccattatgt caatgctccc
    16261 tttagaacaa ttgctccgca aaaacatcca agaacttact ccctactctt gcgcccgcga
    16321 cgagtttcag ggtcaggcat cggtgtatct cgatgccaac gaaaacccat acaacggacc
    16381 ctacaaccgc tatcccgatc ctttgcagcg ggagcttaaa cagaaaatcg cagcgatcaa
    16441 ggggttgcgc cccgggcaag tgtttttggg caacggcagc gatgaatcca tcgatgtctt
    16501 gttcagggct ttttgcgaac cccgcataga caacgtggta gccatagagc ctacctacgg
    16561 catgtacaga gtttccgccg gaatcaacga tgtggcctac cgtagggtct tgctcgacga
    16621 actttttcaa atagatgccg gcagaatttt ggccgcctgc gatgcccgta ctaagcttgt
    16681 ttttctttgt tctcccaaca atcctacggg caacagcctg aacagagccg aaatcctgaa
    16741 attgctggac cgattcgagg ggatactggt gctcgacgag gcctacatcg attttgcttc
    16801 ggagccttct ctgatgggcc ggctggacga ttatcccggc ctggttgtgt tacagacatt
    16861 ttcaaaagcc tggggcagcg ctgccatccg cctgggtatg gctctggcct ctgagtcgat
    16921 agtgaaggtg ttgaacaaga ttaaataccc ctacaacgtt aacctgctca cccaacagca
    16981 agccgcgaag atgttggaat ctgctccccg ggtgaaggat tgggtagcac aaatcctgca
    17041 ggaacgtgaa cggctgcgca aagccctgtc agaactccgt ttggtcaaac acatttaccc
    17101 ctcggacgtc aactttttgc tggtaagggt aagcgatgcc aatcgacttt atactgcttt
    17161 gacagaacaa ggcatcattg tgcgcaacag gaataatgta tctttgtgtc tgggttgcct
    17221 tcgaataacg ataggtacag ccggtgaaaa ccaaatcctg ctacaagctt taaaagcctt
    17281 ggatcaaaac tttagttagt gtctttgtat ataaataata gaattccaat gaaaaaaaga
    17341 gccttgttta tcgatcgcga cggaacatta atcgaagaac ctaagcctag cctgcaggtg
    17401 gacaatttgg atcaattggt gttcttgccc aaagtaatcc ggaatttata ctttatacgc
    17461 aagaaactgg attttgagtt tattatggtg tccaatcaag acggcctcgg cacagatgcg
    17521 tatcccacag aaaatttcga aagggtgcag aataagatgt tacaaatcct gcaaaacgag
    17581 ggcatttgtt ttgatgccat tcacatagac cccagtttgc ccgagcaaaa ttcacccggc
    17641 cggaaaccgg gaactgccat gcttacatcc tatatggacg gaagctacga tctggctgct
    17701 tcttttgtaa taggagacag gatgacggat gttcaactgg cgcataatct gggttgtaag
    17761 tgtatattgt taaacgaaga gataaacctt caggaatcta aggaatatgc cgcaagctgc
    17821 gttatgcaaa gcacggactg ggataaaatc agcgcttttc tttttgccgg ccagcgcaag
    17881 gtcagcatag aacgcaagac caaagaaacc gaggtgaagt tggaactcga tttggacggt
    17941 agcggtaaaa ccaatatcga tacgggcatt aaattttttg atcacatgct cgaccagata
    18001 gggcgacatt cgggttgcga tctgagcata attgtcaagg gcgacttgga ggtggacgag
    18061 catcatacgg tagaagatac cgctctggtt ttgggcgagg ccttgcgtca ggccttgggc
    18121 gacaaacgcg gaattgaacg ttacggtttt tgtttgccaa tggacgatag cctttgcagt
    18181 ctggccctcg attttggcgg ccggccctgg ctggtttggg acgtgaattt ccgcagagag
    18241 aagctgggag agctccccac ggagctgatt tttcacttct tcaaatcctt gagcgattct
    18301 gccaaaatga atctgaatat caaagcttta ggtgacaacg aacaccataa atcagaaagc
    18361 atatttaagg cattcgcatt agcactcaaa atggccatac accgcgatat ataccgttac
    18421 gatttgccca gtaccaaagg cctgctttag tatatttgct ctgtgctttt tcaaataatt
    18481 tgctaccttt gacacctccg gttcactaaa taccaaagca aatgaaacgt atttttttca
    18541 gcttaggctt gttgactctc gtcgcctgtc ccttatttgc ccaaacctca aacctgggcc
    18601 tggcagcaga ccaaccacag gagcagccct ccagatttcc cactgaactc gaagtaagcc
    18661 tttctcccaa ggatattgtg tacgccacaa aagatacctc tgtactcaaa atggacattt
    18721 atcgtcccca ggctcaggat aaagctttgc cctgtctggt gtttttgtat ggaggcggtt
    18781 ttagctccgg ttccaaagac aacaaaacca cggtggaatt ttgtcatcag atgacagaca
    18841 ggggctatgt ggtggtggcc atcaattacc gtcacggtat ggccggcgtc aaaaacaaag
    18901 gtttcaaaat ggttcgaagc ctggaaagag ccatcggtat ggcggtagaa gatttatatt
    18961 cagctacgga ttttttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngccg
    19081 tattgcccat gcagcattat caggacgaaa tagaccgttt tattaaaagc tgcgtattgg
    19141 aaggctgttt ttgtcggaaa gacagcacaa taacagatca cgggctgccc aaggccgatt
    19201 acggaaaatg gagactcagc cggatttacg aataaacctt ctttaccaat aagctctact
    19261 cgagggggct gagagcgatg taaaagagat tgcacacacc cgatttggta atctcgtaag
    19321 aacctgcctc cagcctgtct accacgatga ttttatcgtt gtcttcgtcg gtgtaagcta
    19381 taccgttcag tttcatactg gctgcctcgt tggggccgaa aaccagggtc agccgcctgc
    19441 tgtcggtcag tacaaaggca atgcgggtgc tgctttccat cttgaggcat tcggtgtaaa
    19501 cgctgtcctt gtagttaacc gtacccttgc tgttggaata attgcccgtg acggtgaaga
    19561 aatcgttgga gggccccgcc gaacggctga aagtacagac gatatcgcca ttaccgggct
    19621 cttctgccgg atcacctcca cctcccggat cgtctcctgt ttcaccgccc cccgtttcac
    19681 cgcttcccgg atcgccgttc cctgcctgac cgaagaagcc tatcagactg ctgctgtagg
    19741 aggcaatggc cgctttgagc gcttcgttca cggcgtaaga ggcatcgtcg acggcgttgt
    19801 cgaagctcca ggtgaaatca cccttgccca tgcgtcccgc gccaagcgga ccgcaaacaa
    19861 tttccggcac ctcttcggcc gggtgagggc tgtaggtata catcagggct tcgttaatat
    19921 cgaaattgtt gtaggtatgg ctgcccacca gacttttgta agaagaaggc acgctgtcct
    19981 gtctggaagc agcctcgtag gcgtcaaagc tggtggcgga ctgagtgtgg gggatgaact
    20041 ggagatttgg ggtttcggca aaaacgttcc caaagctctt gatcattcct ccgttttcgc
    20101 ccgaaaaagt gccgtcgccc gtggcatctg ttccctgaag ggaaatcatc atgggtcgtt
    20161 tggtattgcg aaagtaattg gcttcgacaa aaacactagc ccccatggtg gcccccacac
    20221 cgtatttgga aatcccgtcg tagaagttgt tgtacacatg caccgacata acgcgtacac
    20281 ggggatgcct cgaatcggaa tggtcgaacc agttgtgatg ataactgatc cagttttcgc
    20341 ccgattcgcc ggtcatgccg caaagcgagg ctttgcctgc atcccagaaa tgattgtagg
    20401 aaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcgagc gttacgttca
    20521 aagcggcgtc tccggcattg ctttttagta gcaggccgtc ctgatcgctc aacagttgtc
    20581 cggcctcaaa atcttccgtg cggatggttc ctataaaacg caccgcccag ggcgtggagt
    20641 cgtggccttt ttgcttgccc tgtaaaattt ttcctatgcc ggtacgcgtt tcggttttcc
    20701 ctttagaatc tattatcaca ttgtgagtga tgctgttgca attgtctttg gtcacgtaaa
    20761 atactacggc attgtccttg aggctgccgt cgtttttgta tgcgccgaca ccttcgttca
    20821 gattgtggtg ggcaaagccg ctgcgatcgt gggcacttac ttccacaaag tctgattcgg
    20881 ctgcgaaatc gtccatctct aaaccttccc ggtcgagggg aacgatttta aaacgataat
    20941 aaccggcttt caagcctatg gcgtcggctc tgccgtagct gccgtaatta cggatcagcg
    21001 ggctgtccaa aggccggtac tcacttcctg tttcctgcga tttacaatag acccggtagg
    21061 agaggacgct ttccaaaggt tcccagctta cggaagccgc ttcgagccag ccttcggtcg
    21121 cgacgatggc tatggtgttt ggggttgaat cgggttcctg cacaaattga atgccggatg
    21181 cagtgccggg gtaatgcagg ctgcctccct gtttgaaata aatactgaac tcgttgtttt
    21241 cgccaaaaac tatgctttcg agctcttcca ggctgaaatg ctccacgctg tctgtgtcac
    21301 gaagatcgac acgcagtttg gtatttgcct gcaatcctgt caaaacaact acgagcaagg
    21361 caagtgtcag cattttcttc atgacaagcg aatctaaagt ttgacaaaac gaaccaggta
    21421 cttgcccgtc ctgagcaaat agacgccggc aggcagatcg cttaggtcta tctgtaccga
    21481 gcttgcgtga gcttgttgtg ttttgaccag gcggccttcg atattgtaca acagcaggcg
    21541 actgccggtc tcgaggtttt ccagcggcaa gatatccttt accggattgc gggccaataa
    21601 aacacgggtg ttttgcaggg gactgagtcc gcttccctcc gtaaattcaa tgctcaacaa
    21661 caggcaatgg atgctcaggc tgtctccgcc gggaaaatgc aacacggcag tatctccttt
    21721 gaagctgatg cggttaagcg cttgctgcaa ttcctggcct tctatccgca ctattcgctc
    21781 cgaatagata ttcaggctga aaaggcaaaa cagggtcggc agcagaatgc tacggaattt
    21841 catggttgaa gatttatttt ttcggatgac ttgcgcagtt ttccagcaag tagttttcgg
    21901 cctcaatact ccacaagccc cggttcttct cacaaatttc agccagttta cggaaaacgg
    21961 gattgctttc gcctaagcgg gcaaagtcgg aaataggcgt aagaggaaga tcgatgtggt
    22021 tgtatatcag ctttttgcct ccgggaatgg agggtaaatt caaggtggct tctatcacgg
    22081 cattcagtcc gccgatatgt gtaatcagtc ctgccggatc catgccccgg cccataagct
    22141 ccaaggcttc aatcatatcg tcggtattgc ctccgctggt gcccaccaca tgggtgtagg
    22201 cataatgcac gttgtaaaaa ttcatcatgg cgctgaattt gggatcggag ggaccggcaa
    22261 aaaagttcag gcagccgtca aatgccagaa tagcgtcggc ctgttctatc acggaacgca
    22321 cgggcgcaaa aacgaagacg tcgtcgtagc cttcaccgga gctaagttct ttcaatccgg
    22381 ctaccgggtc aggcagggcg gccgtattca cgtaaacaag ctttatgccc ctggaggcag
    22441 caaattcggg cgtgtaaagt ctggcggccc ggtcgagcct ggcctggtca atatcggtca
    22501 ctaccagcaa tgaaggctta cggtcttctc gatgtaacac atagttgatg gccgcaagtc
    22561 ccattgggcc aacaccggcc agaatagcca ttttcccctt gtcgacaatt tccatcttat
    22621 gtacgtagga accgggggtt gtgtggtaat tggcgtgcat ggctccgatc acgcagctca
    22681 ggggctccga aagggaggca ggataaaaac cggcaccttt gtaagataaa aggcagttct
    22741 gctccattac ttcgttgggg attattacat aggtggcatc gcctcctata taggcatagg
    22801 aatagccggg agcactcaaa atacccaccg gacctccttc gtaattcatc gccggttgta
    22861 tggagaattt ttctccggct ttgaatttat gagcccattt agaacctact tcgatcagtt
    22921 ctccggcaaa ttcgtggccg atgatgatgg ggtttttatc cacgtcgtcc ggaacacgtt
    22981 tatgatcagc tccctgtatg gctgccttgt aagaagacat gcacaaactg tcgcacacta
    23041 tttttgccaa tatttcatcg tccttaatgg cgggcaattc aaaagtctcg aggcgaagat
    23101 cttttttgcc gtatagacgt actgctgttg ttttcatctt ggttttcggg taaaaaatta
    23161 atatccgcaa aggtagtaaa aatcctcaag ctctctttgg tggaaataat ataaaaaagc
    23221 ctttttaaaa cacttgtttt tatttacatt tgcaaatctt atcgggcaac gctctaattg
    23281 gcctttatct gcttaagaaa gagcaacaaa gcctgtattt tttggattga aattttcatt
    23341 tttattccta tgtcagacaa taaaagtata cggacagccc tgatttctgt ttatcacaaa
    23401 gaaggtttgg acgaaatttt gcaccaattg caccgggaag gggttgaatt tatctccacc
    23461 ggaggcaccc aacaatttat tgaatcgctg ggttatccct gcgaaaaagt cgaaagtctc
    23521 accggttatc cttcggtatt ggggggcaga gtaaaaaccc tgcatcccaa gatatttggc
    23581 ggcattctca accgccgctc gctggatacc gaccggcagg aatgcgtaga atacgatata
    23641 cctgccatcg acctggtgat agtggatttg tatccctttc aggaaacagt aaaatcggga
    23701 gccgctgccg aagatattat agagaaaata gatataggcg gcatttcgct tatcagggcg
    23761 gctgccaaga acttcaacga tgtggttatt gttgcctcta aagctcaata ccctgctttg
    23821 ctcgatttgc tgaacgagca gggagctacc acttcactcg aggaccgtcg ttggtttgcc
    23881 cgtgaagctt ttgccgtttc atcgggttac gatgccgcca ttttcaactg gttcgacggg
    23941 gcagatggct cagctctgag agttgccatc gaccactcca aggttttgcg ctacggcgag
    24001 aatccccacc agcaagcttc ctttttcggt gatttcgatg agttgtttga gcagctcaac
    24061 ggtaaggaaa tttcttacaa caatctattg gatatagacg cagccgtatc tatggtaaac
    24121 gatttcgacg aaaccacttt tgttatcgtc aaacacaaca acgcctgcgg cctggcgtcg
    24181 cgtcctgtgc tcgtagatgc ctggaatgcc gctctttcgg cagatccggt gtcggctttt
    24241 ggaggcgttt ttgctacgaa cgccgccatc gacaagacta cagccgaaga aatcaacaag
    24301 ctctttttcg aagtattgat tgcgcccgac tacgatttcg acgctttaga aatattgacc
    24361 caaaagaaga atcgcattct tttgattcag aaagaaattc ctcaaggggg aaaacaattt
    24421 cgctccctgt tcaacggagt gttggtgcag gataaggata cacataccga aaccgcagag
    24481 gatttgcgtt gcgttaccga aaaagagagc gatgcagctc agatcgaaga cctgcttttt
    24541 gccaacaagg tggtgaagca caataagagt aacgctatcg tgttggtaaa agacaaacag
    24601 ctttgtgcgg ccggcgtggg tcaaacctcc agggtggatg ccctgaggca ggccatcgac
    24661 aaagcaagga gcttcgacat ggagctcgaa ggggccgtaa tggcttccga tgcctttttc
    24721 cctttccccg attgcgtcga aattgcagat gaagcaggca ttacggctgt aattcatccc
    24781 gggggatcca tcaacgacaa attatccatc gaatattgca acgaacacgg tttggccatg
    24841 tacacaaccg gatacagaca ctttaaacat taaaaccgat atgggaatct tttcttttac
    24901 tcaaaaaata gccatcgatt tgggcactgc caacactatc attatgtgta acgataaaat
    24961 tgtggtagat gagcccagtg tagtagccct cgatcgtaaa accgacaaga tgttggccgt
    25021 aggcgagaaa gcacggctga tgcacgaaaa aactcatgaa aatatccgca ccgtacgtcc
    25081 cttgcgcgac ggagtcatag ccgacttcta tgctgccgag caaatgatcc gcggactgat
    25141 taagatcgct ctgccccggg gcagcggttt cttttcttct ctgcgtatgg tggtttgcat
    25201 tccttcgggc tcaaccgaag tggaactgcg tgccgtgcgc gactcggccg agcacgtagg
    25261 cggacgcgaa gtatatatga tatatgaacc catggcagcc gccattggta tagggatcga
    25321 tgtggaagcc cccgaaggca acatgatagt ggacataggc ggcggcacca ccgaaattgc
    25381 cattatttcg ctgggcggca tagtggccaa caaatccacc cgtatcgccg gtgacgattt
    25441 taccacagac attcaggaat atatgcgccg gcaacacaac ctgcgtatag gggaacgtac
    25501 ggcagaacgc atcaaaatca atgtaggctc tgctctaacg gagctggaaa atcctccgga
    25561 agactttttg gtatacggcc ccaatcaaat gacggctttg cctatggaag ttcccgtttc
    25621 ctatgtggaa attgcccatt gcctcgaaaa gtcggtttcc aaagtagaga ccgcgatttt
    25681 gaatgctttg gagcaaactc ctccggagct ttatgccgac atcgtacgca acggcatcta
    25741 cctgaccggt ggaggatctt tgctcagagg tttggccaaa cgcctgagcg ataagatcaa
    25801 cattcccttt atcgtgcccg aagatccttt acatgctgta gccagaggta ccagcgtaac
    25861 tttgaagaac gtagatagat tcaatttcct gatgcgttaa tgcgacaact tattcatttt
    25921 ctcgtccggt acagaaactt cttcttgttt ttgttcctgg aaatcctgtc gctgatactc
    25981 ttatttcgtt ttaacgacta tcagcaaggg gttttattca gttcagccaa tcgaataaac
    26041 gggaaattgt atactttcag ggaagcggta agtggttttt ttgccctgcg cgaaaacaac
    26101 acggagttgt tacgcatcaa cagtgagatg gaactcaaaa tagctcgtct agaagagagt
    26161 ttgcgcaaag ccgggttgga agaggagcaa ttaaaagccg cgggcagcta cgaattcatt
    26221 catgccaggg ttattaaaaa cagcgtttcg caactgcgaa actacctgac cctaaatgtg
    26281 ggtaagctgg acggagttgc cgaaggcatg ggggtttgca accatctggg catagtgggg
    26341 gtggtgagtg tctgttccga ccatttttcg gtcgttattc cgctattgaa ccccgatatc
    26401 agtttcagtt gtattgtttc cggcagcaac agtgtgggtt cactcaattg gaaaggaggg
    26461 gatacgcgtt ttgcctggct ggaggaattg cccgactatg ctttttgggc tataggagat
    26521 acggtggtaa ccagcggttt ttcggatgct ttcccctacg gaattcccgt tggagtgata
    26581 gaaagcatcg agaggaaaag caacagtagt tttcatcgtt ccaaggtcag gctgtttacc
    26641 acttttgagt ctctggcaga cgtgagggta atttcgcatt ttttgcgtga agaaaaactg
    26701 gaattggaaa aagagcttaa acaatgatac gtttatttct gaaacatctt tttttgtttg
    26761 tactcttgtt tttgctgcaa ataatagtat ttcagcaact ttatttctgg tatgtaacgc
    26821 ccttagtata cgtctatatt cttattcgct ggcctgcgac gcttccggca ggctgggtac
    26881 ttatcctggg ttttttgctt ggcctgttgg tcgatatcaa cgtcgatact cccggcatgc
    26941 acgccgctgc cactaccttt gtggcgtttt tgcggccctc cttactcaaa ggttttctga
    27001 atagagacga ggttgaaaac ctggaaccca gagaaaaaag catgggtttt ctcccttatt
    27061 ggaaatacgc cgtcagtata gtcttgctgc atcataccgc gctctacgta ctggaatctt
    27121 tttccttttt cgaccccttg ctcttgctgg ttaaaattgt cggaagcgcc ttgctgagcc
    27181 ttttgttgat ttttttattc gaacgattct aactgaagac cggacattgc catgcgatca
    27241 tctaccaccg actatgagaa taggggcaga atttttattc tgatcattgt gggcgtgata
    27301 tttattttta ttgtccggct ggcgtatttg cagttgtttt cggaagaata cgacaaatac
    27361 gccgacagca acgcattgtt caaaaaaacc atctatcccg aacgcggagt gatatacgac
    27421 agaaacggca aactgatggt atataatcag gccttttacg atatcatggt tactccccgc
    27481 gaaatgcagg gcctggacac cctggagttt tgttccactc taaagataga tcgcgccttt
    27541 tttgagaaga gaatgacaga cattcgcaac cggaggctta atccgggtta ttcttcttat
    27601 acccctcaac tgtttatgtc gcaattgtct gtcgaagcct atggaccgtt acaagaaaaa
    27661 ctctatcgtt ttccgggatt ttcgattacc aaccgcagtc tgag
//
LOCUS       DAOSTD010000021        25806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_2095_length_25806_cov_15.638383, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000021 DAOSTD010000000
VERSION     DAOSTD010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25806
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2095_length_25806_cov_15.638383"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLI37_02530"
     CDS             complement(<1..325)
                     /locus_tag="PLI37_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOQ57929.1"
                     /translation="MKSINLFFKHKTYNDLVMFFLRKVFSVLVIVAISILAFQSCKEI
                     DQEAYYTFKGETVVSYLENDPNQFSEFSAVLKHTGLDALLATYGTFTCFIPTNDAMRA
                     YYADSS"
     gene            complement(482..1906)
                     /locus_tag="PLI37_02535"
     CDS             complement(482..1906)
                     /locus_tag="PLI37_02535"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOQ57930.1"
                     /translation="MNLMSRLLSSCLLVLFFFAVSCTREYGFEHQGMLRLSTDTLSFD
                     TIFSNVGSTTARIKVYNPYKQKLLISEIGLKTGGNSGFRLSVDGLTSSSCSDLELPPR
                     DSLYLFVEITAALQGVADPVLIEDAVYFVTNGHRQEIVLQAYAQDALVWRGKRIEQDT
                     LLKAELPYLIYDSLVIDSGAVLSVDPGVRLHFHDRAVVVSYGSIKAKGELNNPVIFRG
                     DRLDKLFVDFPYEYYPGQWYGIYLDKESYGNEFDWVEIRGAYYGIIADSSSLEQRKLE
                     INNSVIFNMVYSNLYSIHSNIRVNNSLLANSGSYTLLLLGGDAEFVHCTIANYQRLVS
                     RDGPALALANFLGEEGKASIYYPLQKADFVNSIIYGSQLDEIGLGIAEESAHAFFFRN
                     CLIRNSVELAAEYAEACIYNEDPAFMSTGKDYLYNFRIDSTSAAIDKGLDSVALTIPF
                     DLDGNSRTSDIAPDLGAFEWHGLE"
     gene            complement(2009..3460)
                     /locus_tag="PLI37_02540"
     CDS             complement(2009..3460)
                     /locus_tag="PLI37_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOQ57931.1"
                     /translation="MKTLDNLKPALIWSYFLEITRIPRPSGKTQAMQNWLLDFAKKHK
                     LEHDRDAVGNVIIRKAATKGMEHIDSVVLQSHMDMVCEKNSDVQHDFEKDPIDVYIDG
                     DWLKARGTTLGADNGIGMAAQLAVLASDDLEHGPLECLFTVDEEVGLVGAFNIQAGFM
                     RSKRLLNLDSEDEGMLFIGCAGGINTKAYFEFQKEKAKEKLFFFKLDISGLKGGHSGC
                     DIHEGRGNANQLLARFLWQEAQVNELYLVDFTGGNLSNAIPREASCVAAVPVTQKEQI
                     SVRVNMYEAELRNEFSSMEPGLKFTIESHDAVGEVLKKADSARFIQAVYACPNGVIAM
                     SKEIEGLVETSTNLASVKPLSENSWLITSSQRSSIESSNKDISHRVEAVFRLAGMKVE
                     HNEGYPGWKPNPESELVKIMTKAHKEVLGYEPVLTAIHAGLECGLFLQKYPDLDMVSF
                     GPTMTGVHSPDEKLYIPAVENFWNWLVKSLKQM"
     gene            3606..3800
                     /locus_tag="PLI37_02545"
     CDS             3606..3800
                     /locus_tag="PLI37_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57932.1"
                     /translation="MWRVVFLAVLFVGIAVLLLGIRVFFVKGGKFPNSHIEGNKALRD
                     KGICCAARQDSMSRNKSIKF"
     gene            3808..4422
                     /locus_tag="PLI37_02550"
     CDS             3808..4422
                     /locus_tag="PLI37_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOQ57933.1"
                     /translation="MKRINLISNLILAIAIIVLYVLFLNKDCKSSSSDFVNLNDSGRV
                     ETKLPVAYINLDSLLLDYNYSKDLNEQLLRKRENSQASYNQKARQFEQEAAEFQRKYE
                     NNAFLTQQRLQSEQQRLMQKQQELQQLDEKLSQELANEMQKMNEQLRDTIYSYLKEFN
                     KERNYHLIFSNTMNDNILISCDVYDVTKDVVQALNARYAGSKSK"
     gene            4510..6903
                     /gene="lon"
                     /locus_tag="PLI37_02555"
     CDS             4510..6903
                     /gene="lon"
                     /locus_tag="PLI37_02555"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOQ57934.1"
                     /translation="MISEDESEQALNQTDLPDTLPILPLRNMVLFPGVAMPVSIGRNK
                     SLRLVREAYQKNTIIAVFTQKDAKTEDPEKEHLVPVGIVSRIIRVLEMPDNSTTIILQ
                     AIKRCVLDELTLTEPYLKGRIQIREDILPETEDRQYEALIQAIKDLSVKIIKNSSGNI
                     PQEAIFAIRNIEKPFALVNFICANFAFKIQDKLALLQTDNFKDRCYKLLDIMNKESQL
                     LELKVSIQSKAREDINQQQREYFLQQQIKTIQDELGGSAQDQDIKELKSKARNKKWSA
                     DTLKVFKKEVQKLERMNPQSPDYSTQMNYLQTLLDLPWNEYSKDNFNLNRAKRVLNKD
                     HFGMDKVKERILEHLAVLKLKGDMKSPIICLYGPPGVGKTSLGKSVAESLGRKYLRIS
                     LGGLHDEAEIRGHRRTYIGAMPGRIIKSLIKAGTSNPVFILDEIDKLSRDAQGDPSSA
                     LLEVLDPEQNSTFHDNFIDIDFDLSKVLFIATANTLSTIPQPLLDRMELIEVSGYLTE
                     EKVEIAYRHLLPKELENHGLKAGQLILNKTTLRHIIEKYTRESGVRELNKKIAAVFRK
                     VAHKLASDESLTQIEIKPENLKDYLGNPLYIKDKYEGNDYAGIVTGLAWTSVGGEVLY
                     VESSLSKSKSGGKLSLTGNLGEVMKESAILALEYIKAHANDLDISPELFENYNVHIHV
                     PEGAIPKDGPSAGVTMVCSLASSYTQRKIRKQLAMTGEITLRGKVLAVGGIKEKILAA
                     KRFGIKEIILPEENRKDIDDIQPVYIKGLSFHYVRDIREVLQLALLKEKVSKAQQFQ"
     gene            complement(6910..8061)
                     /locus_tag="PLI37_02560"
     CDS             complement(6910..8061)
                     /locus_tag="PLI37_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOQ57935.1"
                     /translation="MKNNSMKAGIKKLIRKLGFNKSGRPLLTRIDTYIIGKFLGTFFF
                     SIVMILAIVVVFDYNEKFDKFILHEAPARAIAFDYFLNFIPYFGVKFSPLFIFISVIY
                     FTSKMAGNSEIIAMLSSGLSFNRLLRPYFISATLLAIMTFLLSSYVIPPANKIRLQFE
                     DQYVRKVSSDYVRNVQMEIEPGVIIYMERYDDKRKIGYNFFMERYEGQQLASRMTAKR
                     IALDSLNRWRVMEYLIRDFEGLHENISMGASLDTTIMMNPAEFFITKGYSEQLTTTEL
                     RQYLDRQKRRGVANIKEYEVEYQRRFSFPFSVFILTLIGVSLASRKVRGGTGLHLGLG
                     LLISFSYIFFDTLSGTFALSGSATPFVAVWIPNAVYALIAFGLYLQAPK"
     gene            complement(8058..9185)
                     /gene="tgt"
                     /locus_tag="PLI37_02565"
     CDS             complement(8058..9185)
                     /gene="tgt"
                     /locus_tag="PLI37_02565"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008566042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOQ57936.1"
                     /translation="MGFKLVARDKNSRARAGVLQTAHGEIQTPVFMPVGTAGSVKAVN
                     QQELEHQVKARIILGNTYHLYLRPGLQILKAAGGLHKFMSWNKPILTDSGGFQVYSLS
                     HRRKMKEEGVTFQSHIDGSRHLFTPENVVDTQRIIGSDIMMALDECTPGTADYSYARS
                     SMELTHRWLDRGLQRYRETEPLYGYEQLYFPIVQGCVYPDLRRQSAEYVASKDCAGYA
                     IGGLAVGEPAEKMYEMIEVVNEILPQDRPRYLMGVGTPENLLEAIERGVDMFDCVLPT
                     RNGRNASLFTGQGKLNMRNKRWATDFSPIEAEGAADIDRLYSKAYLRHLFIAEELLAL
                     QLASIHNLAFYIRLMSEARQHIINQDFTEWKKQMIDQWAQK"
     gene            complement(9223..10080)
                     /locus_tag="PLI37_02570"
     CDS             complement(9223..10080)
                     /locus_tag="PLI37_02570"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOQ57937.1"
                     /translation="MELAVVSGKGGTGKSSITAALAVMKQKLLLADCDVDAANLYLLF
                     QPEKTLEQVYVSGHTAIIDKDSCTNCGLCMDYCRFDAIHLTEEEVQISGISCEGCFLC
                     SRICPEQAIRMEPEDRSRLYAGNFRYGKMVYGRLFPGEENSGRLVNLVRQQARDIARK
                     NELDLILLDGPPGIGCPVISTITGVDKVLIVTEPSVSGLHDLQRIHELCRHFGLSQTV
                     VINKYDLNPDMCTEIERYCREQGLKLQAKIPFDPQMVEAMVKGQSILEYAPESPAALC
                     IKNHLEELL"
     gene            complement(10055..10942)
                     /locus_tag="PLI37_02575"
     CDS             complement(10055..10942)
                     /locus_tag="PLI37_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ57938.1"
                     /translation="MDKQFSIAVASGKGGTGKTFVSVNLFYALEQTGLRVHLVDCDAE
                     APNDKMFFKTDLVDNKKEIFQQVPVINLQACTFCGRCYEYCRYNALFFVPELQKISVL
                     YDFCHGCGACLVACNDGAISERDDLLGEVNTFVLSQNTALFESRIKVGVHSSVPVLKA
                     AVQQAAGADVLLMDAPPGTSCPFVQTVARADYTVLVTEPTPFGLSDLKQSVQTLSSIG
                     IPCGVVVNKAGLGNREVYRYLEEEHIPLLMEIPFDKELAAMYSGGKIPAAEILVWKDR
                     FTDLYHQIKEQYGISSRQR"
     gene            complement(10972..11343)
                     /locus_tag="PLI37_02580"
     CDS             complement(10972..11343)
                     /locus_tag="PLI37_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOQ57939.1"
                     /translation="MEKNKRLIALPMEDGLLCAHFGHCRSFAIVELQDNTIVSIKEVV
                     PPEHVPGLYPRWVAGFGVTDVIAGGMGEKAIQLFNAQNINVFVGAPQKSAEELVKDFI
                     GNKLELSANYCRHDKNHFCGQ"
     gene            complement(11367..11675)
                     /locus_tag="PLI37_02585"
     CDS             complement(11367..11675)
                     /locus_tag="PLI37_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOQ57940.1"
                     /translation="MKIAITSEGDHLQALVDARFGRCAFFAVYDTETKQTTFHANPAK
                     ELAHGAGPAAVQFVARLGVSRVLSGEFGTKVVPLLQELNIERQQIQPERLDKVIDNLK
                     "
     gene            complement(11702..12538)
                     /locus_tag="PLI37_02590"
     CDS             complement(11702..12538)
                     /locus_tag="PLI37_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ57941.1"
                     /translation="MKAVVLSDNRDSSVLPETEHGLSVYLDRGTYKVLLDTGASDLCI
                     RNAGSLGIGLEDVDCIFISHGHADHMGGLASLLKINTKAKVIISPQALSQRFFSNRKS
                     LREIGIQLLVDQYPGRFVFVREDAFIRPDFQVVKPKKWGFPLPKGNQHLFKDSGRGQE
                     PDDFDHELMACFGSEELFVYCGCAHHGLPNILQSINTKINMPIRAMLGGFHLLDSDEQ
                     HTYETEKELSELAEYLKTTQPNTRFYTGHCTGSGAYSFLKQSLGADLELFHTGYEMEL
                     SI"
     gene            complement(12541..12951)
                     /locus_tag="PLI37_02595"
     CDS             complement(12541..12951)
                     /locus_tag="PLI37_02595"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOQ57942.1"
                     /translation="MPRPKLNRRIAAPPLMMGYKPFGIPRKVLSELYLHFDEFEAIRL
                     LDYLGMTQAEAADKMNVSRPTLTRIYEKARRTIARAFVEGKMIMIEGGQVAFEGEWYR
                     CKRCHRLIKGRENHVPCKDCDSYSDEELYRVNEP"
     gene            complement(13122..14450)
                     /locus_tag="PLI37_02600"
     CDS             complement(13122..14450)
                     /locus_tag="PLI37_02600"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOQ57943.1"
                     /translation="MKLYGILLFFLLLMPCAAQTYRNEIFAEDIRTLEVNKEGQWNAN
                     PVIVLNSGEKIHVSFDQLSHDYKRYAYRLIHCNADWTPSGLNELEYMEGFSLQETYRY
                     SLSEGTLTDYTHYELEVPNDQLKLKLSGNYALLLMDRDRQEEILAVACFSVLERRISI
                     KSEVSRSTDLGYNTAYQQLNFVLNATANQIQRPESDLIILVRQNRRLDNEVFGIQALM
                     TSASEIRYEHLPELIFEGGNEYRRFEISSYKFSGLGVDKIRFQQPCYHAYLIESTSRN
                     TAYSFDKDQNGRFYVRNFDADDNQQTEADYFWVHFFLKAPESLDETGIYLQGDFTYAS
                     FGEPYKMKYNPQSGSYEKSLFLKQGAYNYMYLSKNKAVPEATKNLKTSASSRAYRTST
                     ISAKKNPGTTFPVEGSFWETENEYQIFVYYRPVGADYDALIAYDELSSQL"
     gene            complement(14504..16405)
                     /locus_tag="PLI37_02605"
     CDS             complement(14504..16405)
                     /locus_tag="PLI37_02605"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOQ57944.1"
                     /translation="MDIQSILTPVWDYLKNILAYKEDTPLLFTQFYFWAFFALVFALF
                     SLFYKRKQLRTVFLLFASLFFYYKTSGLCLLILLFCITSDYFLAQGIYRSRKQSGKTF
                     LLVVSVSVNMALMAYFKYAYFFTDLVNSLLGTEFVVQDVFAIVGNKLAGNPVFNVDKI
                     VLPVGISFYIFQTLSYTIDVFRGKLKPLNNILDYAFYVSFFPQLVAGPIVRASHFIPQ
                     IYRDFRLSRMQFGMAVFWILNGLVKKIVLGDYIAINFIDRVFANPLMFSGFENLSALF
                     GYSLQVYADFSGYTDIAIGVALLMGFYLPKNFNSPYKATNASNFWKRWHISLSSWLRD
                     YLYFPLGGNRRATFGTYFWILLIALLVVALSGDIWVFVLVVFLLGTTGLIIFFENREN
                     SLKAIAAVNLMTVMLLGGLWHGASLNFMIWGGLNGVGILLYKYWKNWSPVLRAFLVCT
                     LFAAVLLWYQYQALPILKILLIWTAVLSVGTLIRLFVSVLEKFSPGLDRFYLFSSKGM
                     GMIWGVIQTFVFITFTRLFFRSGSNLDPAEANRIAWRTARDMIDQIGGRWYLELIPQV
                     VWEYRHFFILLVAGLFVHWLPERFKRRYRINFALMPLWLMALVVVATVFVVYQFASAG
                     LQPFIYFQF"
     gene            complement(16441..18006)
                     /locus_tag="PLI37_02610"
     CDS             complement(16441..18006)
                     /locus_tag="PLI37_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57945.1"
                     /translation="MAMLALIGFFFPKEGIQLGTVSLQFRSTRDVFVLSGEQVRAPDP
                     EEQLAAMAESFLKEKLEVLSDSMDYYERFFKESPVRIHFPNDDPAMLDLFFETLDSCR
                     VQKRVVRVLHYGDSQIEQDRLSATLRDGLQKIAGGMGVGMIPAIQTIPSRTLRQEFEG
                     NGKRYVLYGPSSLRAPHKRYGPMAAVCEVEDYAYLSVNALKRQNSDLSNQFTNIRLLL
                     GNNSAGFKATLEIKDKYDTKTVEEAGLDLKVISWELDRPENKFSLFLDGSAEIYALLL
                     DGAYGVAVDNIPMRGSSGNVFTGIDGRLLHAAYRAMDVQLFLLEFGGNRVPSIYSREG
                     INNYSGAIGRQIRYLKTLVPGAQVIFIGPADMSCPVDGKMQSYPLLEEFIEGLKKAVL
                     ENGAAFWDMYQVMGGQNSMINWVNARPPLAAPDYIHFTKKGADKIAELLSENMALCYS
                     YYQFRKKHNPNDSTRIGLSSLERVYSDYAEEFEQVIREKLASADSLPEEPDNEAILND
                     FLELLNKNTNNSE"
     gene            complement(18118..20016)
                     /locus_tag="PLI37_02615"
     CDS             complement(18118..20016)
                     /locus_tag="PLI37_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ57946.1"
                     /translation="MKKKTIVDLFEESCAKYAQNVFLWERDTEFRPTTFAETQKEVYR
                     FGAGLLAMGLKKDEKVALLSEGRNKWMIGELGILYTGAVNVPLSIKLQSGSDLVFRIN
                     HSDSRFILVSGTQLPKVREIMDQMPLIEKVIVMDEDITLQDKELYFEDVCKMGDKFNK
                     KHPGKLLEIAQSVQGDDYANISYTSGTTADPKGILLTHRNYTANVEQSRSRADIPPHY
                     RMLMILPLDHCFAHVTCFYVFMSCGASIATVPVGKTALESLRNIPLSIQEVKPDLMMS
                     VPALAKSFKASVEKGVAAKGPAVQKLFDFAMKVAVAYNKEGYNKGTGGTFVLKPLVKL
                     FDKILFSKLRDALGGNMKFFIGGGALLDIDMQKFWYAIGIPMFQGYGLSEATPVISTN
                     FFTKHKLGSSGVPVIPLELTIRDEKGNILPTGQKGEIVIKGENVMAGYWKNPTATAET
                     IKDGWLYTGDMGAMDEDGFLYVYGRFKSLLIASDGEKYSPEGIEESLVTLSPHIEQVV
                     LYNNQSPYTVALIVVNKELLKKDVPSYDSLEGKKQALKLIQQEINAFKQGGVHEGMFP
                     ERWLPATFAVLPEAFTEQNKLLNSTMKIVRGKVEERYSDRIEHMMTAAGKDIFNEKNI
                     EALRQQLM"
     gene            complement(20030..20617)
                     /locus_tag="PLI37_02620"
     CDS             complement(20030..20617)
                     /locus_tag="PLI37_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOQ57947.1"
                     /translation="MKIDIILAGVGGQGILSIAAVIGEAALSQGLFMKQSEVHGMSQR
                     GGDVQSNLRISDRAIYSDLIPAGKADLVLALEPMEALRYLPYLSAEGWLVSNSVPYVN
                     IPNYPEEDKLKESIARFQRKVILDVDSIAREAGSVRAANMVLLGAACPFLRLDYSGLQ
                     QAVRRIFERKGEAVVELNLKALSMGKALAEQEFKP"
     gene            complement(20614..22212)
                     /locus_tag="PLI37_02625"
     CDS             complement(20614..22212)
                     /locus_tag="PLI37_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOQ57948.1"
                     /translation="MNSKLLLLADEAVAQAALDAGISGVYAYPGTPSTEITEYIQSSD
                     KAQRENIHRNWSANEKTAMEAAMGMAFAGKRALVCMKHVGVNVAADPLMNSSISGVVG
                     GLVFVAADDPSMHSSQNEQDSRFYGRFAMIPVLEPSSQQEAYDMTYYGFELSEKLGFP
                     VMLRITTRLAHSRSGVVRREERAQNKLRFSDDPRQFVLLPVNARQRYKKLLAAQQDMQ
                     ALSEASSFNAFFPGKDLSKGVVACGIAYNYLMENFPEGCPFPVLKVGQYPLPKTAIAQ
                     LLENCGEVVVLEEGYPLAEEMIGGLLNRGNKVTGRLSGLLPRDGELNPDIVAAAFGLK
                     TTQLFEKPEVVMPRPPALCQGCGHRDMYDALNVAIAEYGERVKVFGDIGCYTLGYLPP
                     FKALHACLDMGASITMAKGAADAGLEYAVAVIGDSTFTHSGMTSLLDAVYENSPITVI
                     ISDNEVAAMTGGQDSPGTGRLEAICAGLGVEPEHIRSLVPLKKNHDEMVQVIKEEMNY
                     RGVSVIIPRRACIHTLNRKKNSKQ"
     gene            complement(22351..24219)
                     /locus_tag="PLI37_02630"
     CDS             complement(22351..24219)
                     /locus_tag="PLI37_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOQ57949.1"
                     /translation="MVLTLAILFVTAVFFVWGKIRSDIVALCSLLSLVLLGILKPGEA
                     LAGFSNSVVIMMVGLFIVGGAIFQTGLAKSISLRLLKFAGKSEIKLFLLVVLVTVFFG
                     SFVSNTGTVALLLPIVVSMATEAGTNSRRLLMPMAYASSMGGMMTLIGTPPNMIINDT
                     LIKAGYGSLSFFSFLPVGLMITAIGIVYLFPVSKILTRKKEKSSKTGSVKTPDQLSKE
                     YQLADNLFRIEVSKNSDVINKKLSELNITENYHISILVVRRKDTQDGKFFKPVINQRN
                     SRLVSADTTLLPDDLLYVFGNFEEVKKFVTDHKLSFLDKSVSETSRRPDFSRDIKFDE
                     IGIAEVVVMSNSKLVNKMVKESGFRTNYNVNILGIKRSREYLIYNVKDEKIHSGDALL
                     VQGTWQDIERLNNNEPDVVVVGQPSIEASKVPLTSKAPIAAIIMIAMVVAMVINIVPP
                     VIAVMLAALAMILTGCFRNVEAAYKTINWESIVLFAAMIPMATAMEKTGASQMISGSI
                     VGGLDSYGPYAVMAGIYLATSFLTMFISNTATAVLFAPIALQSALSMGVSPYPFLFAV
                     TVAASMCFASPFSTPPNALVMSAGRYTFMDYVKMGLPLQIIYMIVMVFFIPLIFPF"
     gene            complement(24283..25317)
                     /gene="mltG"
                     /locus_tag="PLI37_02635"
     CDS             complement(24283..25317)
                     /gene="mltG"
                     /locus_tag="PLI37_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOQ57950.1"
                     /translation="MKIKVRLLLTASGLLLLLAVGIIVWIYGVFYRPNVLDGQSRHIY
                     LYEEDDFDSLLQQLKDKNLLRDERSFKKAADYYDYPRQMHSGHYVLEAGMNNKRALQI
                     LRLGLQQPVRIRFHNLRKVEQLAGLLSKQVMADSLSLLEIFYDRVWLDSLGLTAETLP
                     ALFIPDTYEVWWNSSPEKLMQLFSRSYRQFWNEERRAKAEALGLSPVEVSVLASIIEE
                     ESNVKDEWPVIAGLYLNRLRRGMYLQACPTVKFALNDFSIQRVLTEHTEVVSPYNTYL
                     HLGLPPGPIRIPQKSSLLAVLDAEKHNYLYMCAKDDFSGRHYFSSSLAEHNRYAQKYH
                     RALNQRRILK"
     gene            25491..25580
                     /locus_tag="PLI37_02640"
     tRNA            25491..25580
                     /locus_tag="PLI37_02640"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25525..25527,aa:Ser,seq:gct)
BASE COUNT         7270 a         6312 c         5613 g         6611 t
ORIGIN      
        1 tggaagagtc ggcataataa gcacgcatgg cgtcgttggt gggaataaag caggtaaatg
       61 tgccgtaggt agccaacaaa gcatccaaac ctgtgtgttt aagtactgca ctaaattccg
      121 aaaactgatt aggatcgttc tctaaataag aaacaactgt ttcaccctta aaagtatagt
      181 aggcttcctg gtcgatttcc ttacacgatt gaaatgcaag aatcgaaatc gcaacaatta
      241 ccaaaacaga gaatactttc cttagaaaaa acataactaa atcgttgtag gttttatgtt
      301 taaaaaaaag gttgatgctc ttcatagcgg tggttatata aagttttttg ccttaaccat
      361 ataaaaatcg atgcaatttt agtacttttt gccgaatctt taatcatggc cggctcaaaa
      421 aaaggattat ttagcataat ataagaaaaa aactaaatcg gtttagccat atacggccga
      481 tttactctaa tccgtgccat tcaaaagctc cgaggtcggg agcaatatcg gacgtccggc
      541 tgttaccgtc caggtcgaaa gggatggtaa gagcaacact atcgaggcct ttatctatgg
      601 cagccgacgt ggaatctatt ctaaaattat ataaataatc tttaccggta ctcataaaag
      661 ccggatcctc gttgtaaata caagcctcgg catattccgc ggccaattcg acactgtttc
      721 ttataaggca attcctgaaa aagaaagcat gagcactttc ttccgctatt cccaggccta
      781 tctcatccaa ttgtgaaccg tagataatgc tgttgacaaa atcggccttt tgcaagggat
      841 agtaaatcga ggctttaccc tcctctccca aaaaattagc caaggccagg gctgggccgt
      901 cccggctgac caggcgctgg tagttggcaa tggtacaatg tacgaattca gcgtcgcctc
      961 ccagtaaaag caaggtatag gagccgctgt tggccagcag gctgttgttt acccgtatat
     1021 tggaatgaat actgtataag ttggaataaa ccatgttgaa aatcacagag ttgtttatct
     1081 ccaatttgcg ctgctccaaa gaagaagaat cggcgataat gccgtagtag gcgccgcgga
     1141 tttccaccca atcaaactca ttaccgtaac tctctttgtc cagatagata ccataccatt
     1201 gcccgggata atattcgtag gggaaatcca caaacaactt atccaggcga tctcctctaa
     1261 aaataaccgg attattgagc tcgcctttgg ctttaatact accgtatgac accacaaccg
     1321 cccggtcgtg gaaatgcagt cggacaccgg ggtcaacgct cagcaccgca ccggaatcga
     1381 tcaccaggct gtcgtagatc aaataaggca actcggcttt cagcaaagtg tcctgttcaa
     1441 tgcgtttgcc cctccataca agggcatcct gggcataagc ctgcaatacg atttcctgtc
     1501 gatgcccgtt tgtaacaaaa taaacggcgt cttctatcaa tacagggtcg gctactccct
     1561 gcaaagcggc cgtaatttcc acaaacaaat aaaggctgtc gcgcggcggc agttccagat
     1621 ccgaacagct cgaggaggta agtccgtcga cgctcaaacg gaaaccacta ttgccccccg
     1681 ttttcaatcc gatttcggaa atcagcaatt tttgtttgta cggattatac accttgattc
     1741 gggctgtggt agaaccgaca ttcgaaaaaa tagtgtcaaa actgagcgta tcggtcgata
     1801 aacgcagcat accctgatgc tcaaaaccat actcccgagt acaggaaacc gcaaaaaaga
     1861 acagcaccaa caggcaggaa gacaataaac gagacatcag gttcattcca cttctttttt
     1921 atactgagcc ctctgctcaa agcatctata ctaactcctt tgctcaaaac agaactttat
     1981 gcaaaaaact gtagatccca gagccaagct acatttgctt taagctcttt accagccaat
     2041 tccagaaatt ttctaccgca ggaatataca acttctcgtc gggagaatgc acacctgtca
     2101 tggtgggacc aaaggaaacc atatccagat cgggatattt ctgcaggaaa agtccgcatt
     2161 ccaaaccggc gtgtatagca gtgagcaccg gctcgtaacc cagtacttcc ttatgggcct
     2221 tcgtcataat tttaaccaat tccgactcgg gattgggttt ccagccgggg tatccttcgt
     2281 tgtgttccac tttcatgcct gccagtcgga aaacagcttc cacccggtgt gaaatatcct
     2341 tattggacga ttcgatggaa ctgcgttgcg aactggtaat caaccagctg ttttcgctta
     2401 gtggcttaac agaggccaga ttggtcgagg tttccaccag tccctcgatt tctttgctca
     2461 tggcaatcac accgttgggg caggcatata cagcctgtat gaaacgcgcg ctgtcggcct
     2521 ttttcaaaac ctctcccact gcatcatgcg attcgatcgt gaatttaagc ccgggctcca
     2581 tcgaagaaaa ttcatttcga agttctgctt cgtacatatt gacccggacg ctgatctgtt
     2641 ccttttgggt caccggtacg gcagctacac aagaggcttc gcgaggaatg gcgttactca
     2701 ggttgccgcc ggtaaaatcg acaagataca gttcgttcac ttgagcttcc tgccaaagga
     2761 aacgagccaa caattgattg gcattgcccc ggccttcgtg aatgtcacaa ccggaatgtc
     2821 cccctttgag gccggatata tccagtttga aaaagaaaag cttttctttg gccttttctt
     2881 tttgaaactc aaaatacgcc ttggtgttaa tgccgccggc acaaccaatg aaaagcatgc
     2941 cctcgtcttc ggagtcgagg ttgagcaagc gtttggaacg cataaatccg gcctgaatat
     3001 tgaaagctcc taccaaaccg acttcttcgt ccacggtaaa cagacactcg agaggaccgt
     3061 gttcaaggtc gtccgaagca agtacggcca actgggcggc cataccgata ccgttgtcgg
     3121 ctcccagagt ggtgcctctg gctttgagcc agtctccgtc aatgtaaaca tcgatggggt
     3181 ctttttcgaa atcgtgttgt acgtcgctgt ttttttcaca aaccatatcc atatgcgatt
     3241 gcaacaccac ggaatcgatg tgttccatac ctttggttgc cgctttacgt ataataacat
     3301 ttccaaccgc atctctatcg tgttcgagct tgtgtttttt tgcaaaatcc agcagccagt
     3361 tttgcattgc ctgagttttt cccgacggcc tcggaatcct ggtgatttct aagaaataag
     3421 accaaatcaa agccggcttt aaattgtcca gtgttttcat tatatatccg ttaaaatatt
     3481 agttgatttt tccaataaaa atagttaatt ccggatttct tcccacgaaa ttgccacaaa
     3541 aataataaaa cttgagggtt tgaacaatag aatgtcctat ctttgtccgc tcaatcgtaa
     3601 atctcatgtg gcgagttgtt tttttagctg ttttgtttgt cggaatcgct gttctcctgc
     3661 tggggatcag ggtctttttt gtgaaaggcg gcaaattccc caacagccac atcgaaggca
     3721 ataaagccct tagagataaa ggcatttgtt gtgcagctcg ccaggacagc atgtccagaa
     3781 acaagtcaat taaattttaa tttcgatatg aagcgaatca acctaatcag caaccttatt
     3841 ttggctattg ccataatagt gctttatgta ttatttttaa ataaagactg taagtcatca
     3901 agcagcgact ttgtaaatct gaatgactca ggccgggtgg aaacaaaatt acccgtcgcc
     3961 tacatcaacc tcgactcact gcttctggat tacaattatt ccaaagatct taacgagcaa
     4021 ttgttgcgca aaagagaaaa ctcccaagcc agttacaatc aaaaggcccg ccagtttgag
     4081 caggaagcgg ccgaattcca gcgcaaatac gaaaacaacg cctttttaac ccagcaaagg
     4141 ctgcaaagcg aacaacaacg cctgatgcaa aagcaacagg aattacagca actggacgag
     4201 aaactatctc aggaactggc aaacgaaatg caaaaaatga acgagcaatt gagggatacc
     4261 atttattcct atctaaagga gttcaacaag gagcgcaact atcatttaat attcagcaac
     4321 acgatgaatg acaatatact gatctcctgt gatgtatatg atgtcacaaa agacgttgtc
     4381 caggcactca acgctcgtta tgccggctca aaatcaaaat aaatccggtt tagtggaatt
     4441 attgttatga taaatctttt ttcaagacaa ctgttttctt ccgatgagga agagggagat
     4501 tttattccca tgatttccga agacgaatca gaacaggcgc ttaaccaaac ggatttaccg
     4561 gacacactac ccatactgcc cctgcgcaat atggtcttgt ttcccggggt agccatgcct
     4621 gtaagtatcg gccgcaataa atccttgcgt ctggtcaggg aagcctacca aaaaaacacc
     4681 atcatcgccg tctttacgca aaaggatgcc aagaccgaag accccgaaaa agaacatctg
     4741 gtgcccgtag gcatagtttc ccgcatcatc agggtgctgg aaatgcccga taattcaacc
     4801 accatcatcc tgcaagccat caaacgctgc gtactggacg aactcaccct gacggaacct
     4861 tatttaaaag gccgtatcca gattcgcgaa gatattttac ccgaaacgga agaccggcag
     4921 tatgaggcat taatccaggc catcaaagat ttatcggtca aaattatcaa aaattcttcc
     4981 ggcaatattc cacaggaagc catttttgcc atccgcaaca ttgaaaagcc ctttgccctg
     5041 gtcaatttta tctgtgccaa ctttgctttt aagatccaag acaaactggc actactgcaa
     5101 accgacaatt tcaaagatcg ttgctataag ctgctggaca tcatgaacaa agagtcgcag
     5161 cttctggagc tcaaagtctc catacaatcc aaagccaggg aagacatcaa tcaacagcaa
     5221 agagaatact ttttgcagca gcagatcaag actatacagg acgaactcgg aggcagtgcc
     5281 caagaccagg atatcaaaga attaaaaagc aaagccagaa acaaaaaatg gtctgccgat
     5341 accctgaagg tatttaaaaa ggaagttcaa aagctggaac gcatgaatcc gcaatctccg
     5401 gattattcaa cccaaatgaa ttacctccag accttgctcg atttgccttg gaacgaatac
     5461 tccaaagaca atttcaatct aaaccgtgcc aaaagagtac ttaataaaga tcactttggc
     5521 atggacaaag tgaaggaacg cattttggaa catcttgccg tgctcaagct caagggcgat
     5581 atgaaatcgc ccattatctg cctgtacggc cctcccggag tgggcaaaac ttccctgggt
     5641 aaatctgtgg ccgaatcgct ggggcgcaaa tacctgcgta tatcccttgg cggcctccac
     5701 gacgaagccg aaataagagg tcacagacgc acttacatcg gagccatgcc cggccgcata
     5761 atcaaaagtt tgattaaagc aggcacatcc aatccggttt ttattctcga cgaaatcgat
     5821 aaactaagcc gtgacgccca aggcgaccct tcttcggctt tactggaagt gcttgatccg
     5881 gaacaaaact ctacttttca cgataatttc atcgacatag atttcgactt gtccaaggtc
     5941 ttgtttattg ccactgccaa cacactgagc accatacctc aacccttgct cgatcgcatg
     6001 gagctcatag aggtttcggg ctacctgacc gaagaaaaag tagaaattgc ctacaggcat
     6061 ctcctgccta aggaactcga aaaccacggt ttaaaagccg gtcaattgat cctgaataaa
     6121 accaccctcc ggcatatcat cgaaaaatac acccgggaat cgggagtgcg tgaactgaac
     6181 aaaaaaatag ccgccgtctt tagaaaagtt gctcataaac ttgcttcgga tgaatcgctt
     6241 acccagatcg aaattaagcc ggaaaacctg aaagattacc ttggaaaccc gctctacatc
     6301 aaagacaaat acgaaggcaa cgattacgcc ggtatagtca cgggactagc ctggacatcg
     6361 gtgggcggcg aagtacttta cgtggaatcc agcctgagta aaagcaaaag cgggggcaaa
     6421 ctcagcctta ccggcaatct gggcgaagtg atgaaagaat cggccatatt ggctttggaa
     6481 tacatcaagg cccatgccaa cgatctggat atttctcccg agctgtttga aaattacaac
     6541 gtccatatcc acgttcccga aggcgccatc cccaaagacg gtccctcggc cggtgtaacc
     6601 atggtttgct ccctagcctc gtcctatacg caacgcaaaa ttcgcaaaca actggccatg
     6661 accggcgaga ttaccctgag aggaaaagtg ctggccgtgg gcgggatcaa agaaaaaatt
     6721 ctggcagcca agcgctttgg aataaaagaa atcattttgc ccgaagaaaa ccgcaaagac
     6781 atcgacgata ttcagcccgt ctacatcaaa ggcctcagtt ttcactacgt aagagatatc
     6841 cgcgaagtgt tgcaactggc tctgctcaaa gagaaagtga gcaaagctca acaatttcaa
     6901 taatactgtc tatttgggag cttgcaggta aagcccgaag gctatcaacg catacacggc
     6961 attgggaatc cacaccgcca caaaaggggt ggcggaaccc gagagagcaa aggttcccga
     7021 caaggtatcg aaaaatatat acgaaaagct gatcaaaagc cccagcccca ggtgcaaacc
     7081 ggtgcctccc ctcaccttgc gcgaggccag cgacacacca atcagcgtga gaataaacac
     7141 cgagaaagga aaggaaaaac gtcgctgata ttccacctca tattccttga tattggccac
     7201 tcccctacgt ttttgcctgt ccaggtattg cctgagttcg gtggtagtca actgttcact
     7261 atagcctttg gttataaaga actccgccgg attcatcata atagtggtat ccagactggc
     7321 tcccatgctg atgttttcat gcaatccttc gaaatcgcgt atcaggtatt ccattactct
     7381 ccaacgattg agcgaatcca gagcgattcg cttggccgtc attctggacg ccaattgctg
     7441 gccttcgtaa cgctccataa aaaaattgta tccgattttc ctcttatcgt cgtaacgctc
     7501 catatagatg attacgccgg gctcgatttc catctgcaca ttgcggacat aatcggaaga
     7561 aactttgcgt acatattgat cttcgaactg caggcgtatt ttgttggcag gcggaataac
     7621 gtaagaactc agcaaaaaag tcattatggc caacaaagta gccgaaataa aataaggtct
     7681 gagcaagcgg ttaaaactca agccgctgga gagcatggca atgatttcgg aattgcctgc
     7741 cattttcgaa gtaaaataga tgacggaaat aaaaataaag agcgggctga attttactcc
     7801 gaaataaggt atgaagttca gaaaataatc gaatgctatg gctctggccg gggcttcgtg
     7861 tagaataaac ttgtcgaatt tttcgttgta atcaaacacc accacgatgg ccagaatcat
     7921 aacaatggaa aaaaagaaag tacccagaaa cttgccgatg atataggtat ctatacgcgt
     7981 caacaaaggc cttccggatt tattaaatcc cagtttccga attagctttt taataccggc
     8041 tttcattgaa ttgtttttca tttttgggcc cattgatcga tcatctgctt tttccattcc
     8101 gtgaaatctt ggtttataat atgttgccgg gcttccgaca tcaaacggat ataaaaggcc
     8161 agattatgga tcgaggccag ttgcagggcc aacaattcct cggcaatgaa taaatgcctc
     8221 aggtaggctt tggaatacaa acggtcgatg tctgcggcac cctctgcttc tatgggagaa
     8281 aagtcggtag cccaacgttt gtttcgcata ttgagcttgc cttgcccggt gaacaaagag
     8341 gcattgcggc cattgcgggt aggcagcaca caatcaaaca tatccactcc ccgctctatg
     8401 gcttcgagca agttttcggg tgtgcccact cccatcaaat aacgggggcg atcttgaggc
     8461 aaaatttcat tgaccacctc tatcatctcg tacatttttt cggcaggctc tcccacagcc
     8521 aggccgccta tggcataacc cgcacagtct ttggaagcga catattcagc cgattgacgc
     8581 cttaaatcgg gatacacaca gccctgcact atggggaaat aaagctgttc gtagccatac
     8641 agcggttctg tttcgcggta acgttgcagg ccgcgatcca gccagcgatg cgtcagttcc
     8701 atcgagctac gtgcatagct gtaatcagcg gtgccgggtg tacactcgtc gagggccatc
     8761 ataatatccg aaccgatgat gcgctgggta tctaccacat tttcgggggt gaataaatgc
     8821 cgggagccgt cgatatgcga ctgaaaagtg actccctcct ctttcatttt gcggcgatgc
     8881 gagagcgaat aaacctgaaa accaccgctg tcggtgagaa taggcttgtt ccaggacata
     8941 aatttatgca gaccgccggc tgctttgagt atctgcaaac ccggacgcaa atacaaatga
     9001 taggtattgc ccaaaataat ccgggctttt acctgatgtt ccagttcctg ctgattgacg
     9061 gctttgacgc tgccggcagt gcctacaggc ataaaaacag gagtctgtat ctctccgtga
     9121 gctgtttgca gtacgccggc cctggcacga ctgtttttgt ccctggctac taatttaaaa
     9181 cccatgcgcc tgaatttgag gcaaagataa cattttgccc acttaaagca gttcttccag
     9241 gtggtttttt atgcaaaggg ctgccggcga ttcgggagca tactccagaa tgctttgtcc
     9301 tttgaccatg gcttctacca tttgcggatc gaaaggaatt ttagcttgca gcttcaggcc
     9361 ttgctcccgg caatagcgtt ctatctcagt acacatatcc ggattaaggt cgtatttgtt
     9421 tataactacc gtctgactga gcccgaaatg tcgacacaac tcgtggatac gctgcaaatc
     9481 gtgcaagccc gacacgctgg gttcggtgac aataagaacc ttatccaccc cggtaatggt
     9541 agaaatcacc ggacaaccga taccgggagg tccgtccagt aagataagat ccaactcgtt
     9601 tttgcgggca atatccctgg cctgctgcct gaccagattt accaggcgac cggaattttc
     9661 ttcaccggga aacaacctgc catagaccat ctttccgtaa cggaaattgc ctgcgtacaa
     9721 gcggctcctg tcttcgggct ccatacggat agcctgctca ggacaaatcc gggaacaaag
     9781 aaaacatcct tcgcaggaaa tcccggaaat ttgtacttct tcctccgtca ggtgtatggc
     9841 atcgaaacgg caataatcca tacaaagtcc gcagttggta caagaatcct tgtctatgat
     9901 tgctgtgtgg cccgaaacat agacttgctc gagggttttt tcgggttgga acaaaaggta
     9961 aagattggcg gcatccacgt cgcaatcggc cagcaagagt ttctgtttca ttactgccag
    10021 agcggctgtg atgctgcttt tgcctgttcc tcccttaccg ctgacgactg ctaattccat
    10081 actgttcttt tatttgatga tacaagtcgg taaagcgatc tttccacacc agaatttcgg
    10141 cggcggggat ttttccgccc gaatacatag cggccaactc tttgtcaaac ggaatttcca
    10201 ttaggagcgg aatatgttcc tcttccagat aacggtaaac ctcccggttg cccaagccgg
    10261 ctttattgac caccacaccg cagggaatac cgatgctcga caaggtctgt accgactgtt
    10321 tcaggtcgct caaaccaaaa ggagtaggtt cggtaaccaa caccgtgtaa tcggcccggg
    10381 ctaccgtctg aacaaaagga caggaagtgc cgggaggagc atccatcaat aagacatcgg
    10441 ctccggcggc ttgctgaacg gccgctttca gtacgggcac cgaagagtga actcccacct
    10501 tgattctcga ttcaaataag gcagtgtttt gggagaggac gaaagtattg acttcgccca
    10561 gcagatcatc tctttcgcta atggcaccgt cgttgcaggc aaccagacag gcaccgcaac
    10621 cgtggcagaa atcatataaa acggagattt tttggagctc cggaacaaaa aacagagcat
    10681 tgtaacggca atactcgtaa caacgcccgc aaaaagtaca ggcttgcaag tttatgaccg
    10741 gcacttgttg aaaaatttct tttttgttgt caacaaggtc ggttttaaaa aacatcttgt
    10801 cgttgggagc ttcggcatcg caatcgacca aatgaactct gaggcctgtt tgctccaaag
    10861 cataaaaaag attcaccgaa acaaaagtct tgcccgtgcc tcctttgccg ctggctacag
    10921 cgatactgaa ttgtttatcc atcccggtgc ttttgatacc tgagcgcctg tttactgccc
    10981 gcagaaatga tttttgtcgt gacggcagta gtttgccgaa agttccaact tattgccgat
    11041 aaaatccttt accaattcct cggccgattt ttgcggagcg cctacaaaaa cgttgatgtt
    11101 ttgagcattg aacaattgta tggctttttc tcccatacct ccggcaataa cgtctgttac
    11161 accgaatccg gccacccagc ggggatacaa accgggcacg tgctccggag gaacgacttc
    11221 tttgatagat acgatcgtgt tatcctgcaa ttcaacgatg gcaaacgacc gacaatgtcc
    11281 gaagtgggca cacaacaagc cgtcctccat gggaagagcg ataagtcttt tatttttttc
    11341 catattctta ggttttactc ctgtttttac ttcaaattat caatgacttt atccagtctt
    11401 tcaggctgta tttgctgcct ttcgatgttc aactcctgca gcagaggtac aactttggtt
    11461 ccgaactccc cggaaagcac acggcttacc cccagacggg ccacaaactg aaccgctgcc
    11521 ggaccggcac catgggcaag ttctttagcc ggattggcgt ggaaggtagt ttgtttggtt
    11581 tcggtgtcgt acacagcaaa aaaggcgcag cgcccaaaac gagcgtcgac taaggcttgt
    11641 aaatgatcgc cttcggatgt aatagcaatt ttcataattc tgtaaatatt aaaatgttaa
    11701 tttaaataga tagttccatt tcatagccgg tgtggaaaag ttccaagtct gcacccaggc
    11761 tttgtttcaa aaaagaataa gcccccgacc cggtgcaatg acccgtgtaa aaacgagtgt
    11821 ttggctgagt ggttttcaaa tactccgcca attcggacag ctctttttct gtttcgtagg
    11881 tatgctgttc atcgctgtcc aataagtgaa aaccgccaag catggccctt atgggcatat
    11941 taatcttagt gttgatgctt tgtaaaatat tcggcaaacc gtgatgagcg caaccacaat
    12001 acacaaaaag ctcttcgctg ccgaaacaag ccatcagttc gtgatcgaaa tcgtcgggct
    12061 cttgtcctct tccgctgtct ttaaataaat gttgattgcc cttgggcaaa gggaaacccc
    12121 acttttttgg ctttactacc tgaaaatcgg gccttataaa agcatcttcg cgcacaaaaa
    12181 caaaacggcc gggatactga tcgaccaaga gttgaatacc gatttccctt aaacttttcc
    12241 gattggaaaa aaaacgttga gagagtgcct gaggacttat aataactttg gctttcgtgt
    12301 tgattttcaa caaagaggct aaaccgccca tatggtcggc gtgtccgtga gaaataaaaa
    12361 tacaatcgac atcctccagg ccgattccca aactcccggc gttacgaata cataaatcgg
    12421 aagctccggt atcgagcaac actttgtaag ttcccctatc cagatatacc gacaatccgt
    12481 gttccgtttc gggcagaacg gaagaatcgc gattatcgga caggactacg gctttcataa
    12541 tcagggttca tttacacgat agagttcttc gtcggaataa ctgtcgcagt ctttgcaagg
    12601 gacatgattc tcccggcctt taatcaggcg atggcagcgc ttgcatctgt accattcccc
    12661 ttcgaatgcc acctggccgc cttcaatcat tatcattttc ccctccacaa aagcccgggc
    12721 aatggttctg cgggcttttt cgtaaatacg agtcagtgtg ggccgagata cattcatttt
    12781 atcggcggct tccgcctggg tcatccccag gtaatcaagc agccttattg cctcaaattc
    12841 atcaaaatgc aaataaagct cgctcaagac ttttcgcgga attccaaaag gtttgtagcc
    12901 catcatcaag ggcggtgcag caattctacg atttagttta ggtcgaggca tggttttata
    12961 ataatagaag cagttttata taatgagagc ggttccacat aatagaagtg gtcttgtacg
    13021 actatacgtg gtttcacata ttgaacggca aagataagag gaattttgaa cttatgttca
    13081 tttcttcccc tatttatttg ttaaattccc ttaggtaaac actagagctg agaactcaat
    13141 tcatcgtaag caatcagcgc atcgtaatct gctcctacag gccggtaata cacaaaaatc
    13201 tggtattcgt tttcagtttc ccaaaaactt ccttccaccg gaaaagttgt accagggttt
    13261 ttctttgcgg aaatggttga agttctataa gccctggagc ttgcagaagt ttttaagttt
    13321 tttgttgcct ccggaacagc cttattctta gataaataca tataattata ggctccctgc
    13381 ttcaagaaca gacttttctc gtaactgccg ctctgtggat tatatttcat tttgtatggc
    13441 tcgccgaaag aagcataagt aaaatcaccc tgtaaataaa tacctgtttc gtccaaggat
    13501 tcgggggctt tcagaaaaaa atgcacccaa aaataatcgg cctcggtttg ttgattatca
    13561 tcggcatcaa aattcctcac ataaaaacgc ccgttctgat ctttgtcgaa actataagcc
    13621 gtattgcgtg aggtcgactc tatcaaataa gcgtggtagc agggctgttg aaacctgatt
    13681 ttatctactc ccaaacccga aaatttatag ctgctgattt caaaacgccg gtattcgttg
    13741 cctccttcaa aaattaattc gggcaaatgt tcatagcgaa tttcactggc agaagtcatc
    13801 aaagcctgta tgccaaaaac ctcattgtcc aaacggcggt tttgtcttac cagtatgatt
    13861 aaatcgctct cgggccgttg aatttggttg gctgtggcat ttaatacaaa attcagttgc
    13921 tgataggctg tattatagcc taaatcggta gatctgctta cttcggattt gatactgata
    13981 cgacgctcca atacagaaaa acaggcaacg gccagtattt cttcctgccg gtctctgtcc
    14041 atcaacagca gagcataatt acccgacaat ttgagtttga gttgatcgtt gggtacttcc
    14101 agttcgtaat gggtataatc cgtcagtgta ccctcggaca aagaataccg gtaagtctcc
    14161 tgcagcgaaa agccttccat gtattccagt tcgttcaaac cggacggtgt ccagtccgca
    14221 ttgcaatgta tcaacctgta agcgtaacgt ttgtaatcgt gcgacagttg gtcgaaagaa
    14281 acatgaattt tttcgccgga attcaataca ataaccggat tggcattcca ttgtccttct
    14341 ttattaacct ctaaagtacg aatgtcttct gcaaagatct cattacggta agtctgcgcg
    14401 gcacagggca tcaacaataa gaaaaacagc aaaattccat ataatttcat aacggccttc
    14461 atgtttggtt ttacgacgga aaggcaaata taatgaaagg actttagaac tggaaataga
    14521 taaaaggctg tagaccagcg ctggcaaact gataaaccac aaagacagtc gcaaccacca
    14581 ccaaggccat caaccacaag ggcatcaggg caaaattgat tctgtaccgg cgtttaaaac
    14641 gttccggcag ccagtgcaca aacaatccgg cgacaagcaa tataaagaaa tgacgatatt
    14701 cccacaccac ctgaggaatc aattccaaat accatcgtcc cccaatctga tctatcatat
    14761 cgcgtgccgt gcgccaggca atacgattgg cctcggccgg atccaggttg gagcccgaac
    14821 ggaaaaacag acgggtgaaa gtaataaaaa caaaggtctg tattacgccc caaatcattc
    14881 ccatcccctt ggacgaaaac aaataaaacc ggtctaagcc cgggctgaac ttttcaagta
    14941 ccgacacaaa caaacggatc aaggttccca cactgagcac agccgtccat atcagcagta
    15001 ttttcaatat gggcaaagcc tggtattggt accagagcaa tacggcagca aacaaagtac
    15061 ataccaggaa agctctcaac accggagacc agtttttcca gtatttgtac aacaaaattc
    15121 caactccgtt caatcctccc catatcataa aattcaggct ggccccatgc cataaacctc
    15181 ccaacagcat cacggtcatc aagttgacgg cagcaatggc ttttaaactg ttctctctgt
    15241 tttcgaagaa aataatcagg cctgtcgttc ccagtaaaaa gactaccaac acaaacaccc
    15301 agatatcacc gctcagcgcc acaaccagta gagcaatcaa cagtatccaa aaataagtcc
    15361 caaaagtggc ccgtcgatta cctcccaatg gaaaataaag ataatccctt agccaggaag
    15421 aaagtgaaat atgccaacgc ttccaaaaat tggaggcatt ggttgccttg taaggagaat
    15481 tgaagttctt aggcagataa aaacccataa gcaaggccac ccctatggca atatcggtat
    15541 aaccggaaaa atcggcgtaa acctgaagtg aataaccaaa aagcgccgac aaattttcga
    15601 atcccgaaaa catcaaagga ttggcaaaaa ctctgtcgat aaaattgatc gctatgtaat
    15661 cgcccaatac aatcttcttg accaaaccgt tgagtatcca aaagaccgcc attccaaatt
    15721 gcatacggga cagtctgaaa tccctgtaaa tttgaggaat aaaatgagaa gccctgacga
    15781 taggcccggc tacgagctgg gggaaaaaac tgacgtaaaa agcataatcc aagatgttgt
    15841 tgagcggttt taattttccc ctgaatacat caatggtata actcagggtc tgaaaaatat
    15901 agaaactgat cccaaccggc aacactatct tatcaacgtt aaacacagga tttccggcca
    15961 acttgttccc aacaatggca aaaacatcct gcaccacaaa ttcggttccc aacaaggaat
    16021 ttaccagatc ggtaaaaaaa taggcgtatt taaaataggc catcaaagcc atatttaccg
    16081 atacactgac taccagtaaa aaggtttttc ccgattgttt gcgggaacga taaatgcctt
    16141 gagccaaaaa atagtcggaa gtaatacaaa acagcaggat caacaggcat agaccgctgg
    16201 ttttgtaata aaaaaacaag ctggcaaaaa gcaaaaaaac agtacgcagt tgcttgcgtt
    16261 tatagaacaa actgaataaa gcaaagacca aagcgaagaa agcccaaaaa taaaattgcg
    16321 taaacaacaa gggagtatcc tccttgtacg ccaatatatt tttaaggtag tcccaaacag
    16381 gggtcaaaat gctttgaatg tccattaatc tgcttagtct taagccttcg ggcaattgat
    16441 ttattcgcta ttgtttgtat tcttgttcaa taattccaaa aaatcattca agatcgcctc
    16501 attgtccggc tcctccggca gactatctgc agaagccagc ttttcgcgaa ttacctgttc
    16561 aaattcttcg gcatagtcgc tgtaaacccg ttccaagctg cttaacccaa tacgggtgct
    16621 gtcgttggga ttatgttttt tgcgaaattg ataatatgag taacaaagag ccatgttttc
    16681 gctgagtaac tctgcgatct tatcggctcc ttttttggtg aaatggatgt aatcgggggc
    16741 ggccaaaggc ggccgggcgt tcacccaatt gatcatggaa ttttgcccgc ccatcacctg
    16801 atacatatcc caaaaggcag caccgttttc caaaaccgcc tttttgagac cctcgataaa
    16861 ttcttccaat agaggatagc tttgcatttt accgtccacc ggacaagaca tatccgccgg
    16921 gccgataaaa atcacttgcg caccgggtac cagcgttttg aggtaacgga tctgacgtcc
    16981 aatggcaccg gaatagttat tgataccctc ccgggaatat atggaaggca cacggttgcc
    17041 gccaaattcg agcaaaaaca actgcacatc catagcccgg taggcggcgt gaagtaagcg
    17101 accgtcgatt ccggtaaaca cattccccga actgcccctc atgggaatat tatcgaccgc
    17161 cacgccgtag gccccatcca acaacagggc gtagatttcg gccgaaccgt ctaaaaacaa
    17221 gctgaatttg ttttccggac gatcgagctc ccagcttatt acttttaaat cgagaccggc
    17281 ttcttcaacg gttttggtgt cgtatttatc ttttatctcg agagtggcct tgaagccggc
    17341 agaattattt cccaacaata aacgtatgtt ggtaaactga ttggaaagat cggaattctg
    17401 acgtttgagc gcattcacac tcaaataagc ataatcctcc acttcacaaa ccgcagccat
    17461 ggggccgtag cgcttgtgag gagctctcag gctcgagggg ccgtaaagaa cgtaacgttt
    17521 gccgtttcct tcaaattcct gccggagagt tctggaagga atggtttgta tcgcgggaat
    17581 catacccact cccatacctc cggcaatttt ttgcaggccg tctctcaggg tagccgacaa
    17641 acgatcctgt tctatctggg agtcgccgta atgcaacacc cgtactactc ttttttgaac
    17701 ccggcaagaa tccaaggttt caaaaaacaa gtcgagcatg gccggatcgt cgttgggaaa
    17761 atgtatgcgt acggggcttt ctttgaaaaa acgctcataa tagtccatcg aatcggacaa
    17821 tacttccagt ttttctttca aaaaagattc tgccatggcg gccagttgtt cttcggggtc
    17881 gggagcccta acctgctccc cggaaagaac aaatacatcc cgggttgaac ggaattgcag
    17941 actgacagtg cccaattgaa ttccttcttt ggggaagaag aacccaatta aggccaacat
    18001 agccatcaca acgaggatga aacaaagtat tctccggaca gacataaggt tggatcaagc
    18061 cctttattaa ggggcttgtg taaatgaaac aaagtaaaat aagtttccgc tcaaattcta
    18121 catgagctgt tgccgcaaag cctcgatgtt tttttcgttg aaaatatctt tgccggcagc
    18181 cgtcatcata tgttctatgc ggtctgagta cctttcctcc accttaccgc gaacaatctt
    18241 catggtagag ttcaacaact tattctgctc ggtgaaggct tcgggcaaaa cggcaaaagt
    18301 ggcaggcaac cagcgctccg gaaacatccc ttcgtgtact cctccttgtt taaaggcatt
    18361 tatttcctgc tgaatcagtt ttaaggcttg ctttttgcct tcgaggctgt cgtaagaggg
    18421 tacgtctttt ttgagcagtt ccttgttgac tactatcaag gccaccgtat aagggctttg
    18481 gttgttgtac agtaccacct gctcaatatg cggagacaaa gtaaccagag attcttctat
    18541 gccttcgggg ctgtactttt cgccatcgct ggcaatcagt aagcttttga agcgtccgta
    18601 cacgtataga aagccatctt catccatagc tcccatgtcg ccggtgtaca gccagccgtc
    18661 tttgatggtt tcggcagttg ccgtcggatt cttccaataa ccggccatta cattttcgcc
    18721 tttgatcaca atttcgccct tctgcccggt aggaagaatg tttccttttt cgtcgcggat
    18781 ggtcagctcc agaggaataa ccggaactcc cgaagagccc agtttgtgct tagtaaagaa
    18841 attggtcgaa ataaccggag tggcttccga tagaccgtaa ccctgaaaca tgggaatgcc
    18901 gatggcatac cagaattttt gcatatcaat gtcgagcagg gcgcctccgc caatgaaaaa
    18961 cttcatatta cctcccagag catcccgcaa cttcgagaac aaaatcttat cgaagagttt
    19021 caccaaaggt ttcagaacga aagttcctcc ggtgcctttg ttgtagccct ctttattgta
    19081 agctacggct actttcatgg caaaatcgaa taatttttgt acggccgggc ctttggcagc
    19141 cacccctttt tccacgctgg ctttgaaact cttggccaaa gccggtaccg acatcatcaa
    19201 atcgggtttg acttcctgaa tagacaaggg aatatttctc aaagactcca gggcggtttt
    19261 gcccaccggt acagtggcga tactggctcc gcaactcata aaaacataaa aacaggtcac
    19321 gtgagcaaaa cagtggtcca gaggcaggat catcagcata cggtaatgcg ggggaatatc
    19381 ggcccgggag cgcgactgtt ccacattagc cgtataattg cgatgcgtca gcaaaatacc
    19441 cttgggatcg gccgttgtcc ccgatgtata ggaaatattg gcgtaatcgt cgccctgaac
    19501 gctttgggcg atttcgagca atttgcccgg atgcttctta ttgaacttat cccccatttt
    19561 gcaaacatcc tcaaagtaga gctccttgtc ttgcagggtt atgtcctcgt ccatgacgat
    19621 caccttttcg atcaaaggca tttgatccat aatttcgcga actttgggca gttgagtccc
    19681 cgaaaccagt ataaaacggg aatcggaatg attgattctg aataccaggt cgcttccgct
    19741 ttgcagttta atggacaagg gcacattgac ggctccggtg tacaaaatac ccaactcgcc
    19801 tatcatccat ttgttgcggc cttccgaaag caaagccact ttttcatctt ttttcaggcc
    19861 catggccagt aatccggctc cgaaacgata aacttctttt tgggtctcgg caaaagtggt
    19921 aggccggaat tccgtatccc gttcccatag aaaaacattc tgcgcgtatt tggcacaact
    19981 ttcttcaaac aagtctacaa tggttttctt tttcatgact gttatgattt tatggtttaa
    20041 attcttgctc ggctagggcc ttgcccatag ataaagcttt gaggttgagc tcaaccaccg
    20101 cttcgccttt acgttcaaaa atacgtctga cggcttgttg caagcctgaa taatcgaggc
    20161 gaagaaaggg acaggcggct cccagcaaca ccatattggc agctctgacc gagccggctt
    20221 cgcgggcaat actgtctaca tccagaatca ctttgcgctg aaagcgcgct atgctttcct
    20281 ttagtttatc ttcttcggga tagttgggaa tattcacgta aggcacggaa ttgctgacca
    20341 gccatccttc ggcggagaga tagggcaaat agcgcaaggc ttccataggt tccagggcaa
    20401 gtaccaggtc ggctttgccg gcaggtataa gatcggaata aatagccctg tccgaaatac
    20461 gaagattgga ctgaacgtcg ccaccccgct gactcatacc gtgcacttcc gattgtttca
    20521 taaacaaacc ctggctcaaa gcggcttcgc cgataacggc ggctatggat aaaatgcctt
    20581 gtccgcccac tccggccagt ataatatcta ttttcattgt ttgctgtttt ttttacgatt
    20641 cagggtatgt atgcatgctc ttcggggaat aatgaccgaa acaccccggt agttcatttc
    20701 ttctttgatc acctgcacca tttcgtcgtg gttttttttg aggggcacca gacttctgat
    20761 gtgttcaggc tccactccca ggccggcaca aatggcttcc agcctcccgg tgccgggaga
    20821 atcctggcct ccggtcatgg ctgccacttc gttatccgaa ataatcacgg ttatgggcga
    20881 attttcatac actgcatcga gcaaagaagt catacccgaa tgagtgaaag tggaatcgcc
    20941 tatcacagca acggcatatt ccaggccggc atcggcagcg cctttggcca tagtaatgga
    21001 agctcccata tccaaacagg catgcaacgc cttgaaagga ggcaaataac ccagcgtata
    21061 gcagccgatg tctccgaaaa ctttcacccg ttccccatat tcggcaatgg caacgttcag
    21121 ggcatcgtac atatcgcggt gcccgcaacc ctggcacaaa gccggaggac ggggcattac
    21181 cacttcgggt ttttcgaaca gttgagttgt tttcaagcca aaggcggccg ccactatatc
    21241 gggattgagt tccccgtcgc ggggcaacag gccgcttaaa cgtcccgtca ccttattgcc
    21301 tcggttcagc aaaccaccga tcatttcttc ggccaggggg tagccttcct ccaacacaac
    21361 tacctctccg cagttctcca acaattgggc aatcgccgtt ttgggcaaag ggtattgacc
    21421 taccttcagc accggaaagg gacaaccttc cggaaaattt tccatcaggt aattataagc
    21481 tatgccacag gccacgacgc ctttggacaa atccttgccg gggaaaaaag cgttaaacga
    21541 cgaagcctcc gaaagagcct gcatatcctg ttgggctgcc agtagttttt tgtagcgctg
    21601 cctggcgttc accggcagca gcacaaattg ccggggatcg tccgaaaagc ggagtttatt
    21661 ttgtgctctt tcttcgcggc gaaccactcc cgaacgggaa tgagccaggc gggtagtaat
    21721 cctgagcatc acgggaaagc ccagtttttc ggagagttca aaaccataat acgtcatgtc
    21781 gtaggcttct tgttgactgg aaggttccag caccggtatc atggcaaagc gaccgtaaaa
    21841 cctcgagtcc tgttcatttt gcgaagaatg catggaggga tcgtcggccg ccacaaaaac
    21901 aaggccgcct actacccccg atatggaaga attcataagg gggtcggccg ccacgttgac
    21961 tcccacgtgc ttcatacaaa ccagggcgcg cttgccggca aacgccattc ccatggcggc
    22021 ttccatggcc gttttttcgt ttgcagacca gttgcggtgt atgttttctc tttgtgcttt
    22081 atctgaagac tgtatgtatt cggtaatctc ggtagatgga gtgccgggat aggcataaac
    22141 acccgatatt ccggcatcga gggctgcctg agccacagcc tcgtcggcca gtaataataa
    22201 tttactattc attcatctga gtttttacaa acggcaaaag taataaaatt tactttatga
    22261 tgtgtcgttg atttaaagcc ctgtggtatt ttaggattgg tgcactgttg gagatcgctg
    22321 cactgtttaa gatcgctgca ctgtttgaga ttaaaaagga aatatcaacg gaataaagaa
    22381 caccatgaca atcatatata taatctgcaa gggcaatccc atttttacat aatccataaa
    22441 ggtatatctt cctgccgaca tcacaagcgc attgggcgga gtggaaaacg gacttgcaaa
    22501 gcacatgctg gccgccaccg taacggcaaa taaaaacgga taaggactca ctcccatcga
    22561 aagcgccgat tgcaaggcaa tgggggcgaa aagcaccgct gtcgccgtgt tactgataaa
    22621 catggtgaga aaagaggttg ccaggtaaat accggccatc acggcatagg ggccgtagct
    22681 gtctaatcct cccacaatgc ttcccgaaat catctgcgaa gcgccggttt tttccatggc
    22741 tgttgccatc ggaatcatgg ccgcgaaaag tacgatactt tcccagttga tggttttata
    22801 ggcggcttcc acatttcgga aacagccggt cagaatcatt gccaaagccg ccagcattac
    22861 cgctattaca gggggtacaa tgttgattac catagcaaca accatggcta tcataataat
    22921 ggcagctatc ggcgctttag aggtaagagg tactttcgat gcttctatcg agggttgccc
    22981 gactactacc acatcgggct cattattatt caggcgttcg atgtcctgcc aggttccctg
    23041 caccagcaaa gcatcgccgg aatgaatttt ctcgtctttc acgttgtaga taaggtattc
    23101 ccggcttctt tttataccaa gaatattaac gttgtagttg gttctgaaac ccgattcttt
    23161 taccattttg ttgaccagct tggagttaga cattaccact acttcggcta tacctatttc
    23221 gtcaaattta atatctctcg aaaaatcggg acgccggctc gtttccgaaa cgcttttatc
    23281 caaaaagctc aatttatgat ctgttacaaa cttctttact tcctcaaaat tcccaaaaac
    23341 atagagcagg tcatcgggca gtaaagtagt atcagcgctt actaaccggc tgttcctctg
    23401 gtttatcacg ggtttgaaaa acttaccgtc ttgggtatct ttacgcctta ccacgagaat
    23461 agaaatatgg taattctccg taatattcag ctccgaaagt tttttattaa tcacatccga
    23521 atttttggaa acctcgatcc tgaacaagtt atcggccaat tgatattctt tactcaattg
    23581 atcgggtgtt tttacggagc ccgttttgga cgatttttct tttttacggg tcagtatttt
    23641 tgaaacagga aagagataaa cgatacctat tgccgtaatc atcaacccga cgggcaggaa
    23701 cgagaaaaac gacaaactcc cgtagccggc tttgatcaat gtatcgttga taatcatgtt
    23761 gggcggcgta ccgatgagcg tcatcatacc gcccatactg cttgcgtagg ccataggcat
    23821 gagcaggcgg cgcgagttgg taccggcttc ggtggccatg ctcacaacaa tcggcagcag
    23881 gagtgccacg gtgcccgtgt tgctcacaaa ggagccgaaa aacacggtaa ccagcaccac
    23941 gaggagaaat agtttaattt cacttttccc ggcaaatttc aataagcgaa ggctgataga
    24001 cttggcaaga ccggtctgaa aaatggctcc tccaacaata aaaagcccca ccatcataat
    24061 taccacagag ttggagaatc cggccaatgc ctcgccgggc ttgagaattc cgagtaaaac
    24121 caacgacaac aaggaacaca aggcaacgat atcggaacgg atttttcccc atacgaaaaa
    24181 aacggctgta acaaacagaa tagcaagggt aagaacaatg ggagaagaca tagtatgatt
    24241 tattattcgc ctgagttata taagcggcaa aagtaataaa ttttacttta agatacgccg
    24301 ttgatttaag gctctatggt atttttgagc atagcgattg tgttcggcca gacttgaaga
    24361 aaaataatgc cgtcccgaaa aatcgtcttt ggcgcacata tacaaataat tgtgtttttc
    24421 ggcatcgagc acagccaata aagaactttt ttggggaatg cgaatcggac cgggaggtaa
    24481 acccaaatgc aggtaggtgt tatacggaga aaccacttcg gtatgctccg tgagtacacg
    24541 ttgaatacta aagtcgttca gggcgaactt taccgtggga caggcttgca aatacattcc
    24601 tctgcgcaaa cggttcaggt aaaggccggc aataacgggc cattcatcct taacgttgct
    24661 ctcctcctct ataatggaag ccaacaccga cacctcgacc ggactcagtc ccagggcttc
    24721 ggcttttgcc ctgcgctctt cgttccagaa ttgtcggtaa ctacgcgaaa ataattgcat
    24781 cagtttttcg gggctgctgt tccaccacac ttcgtaagta tccggaataa acagggccgg
    24841 cagcgtttcc gccgtcaaac cgaggctgtc gagccataca cggtcgtaaa aaatctcgag
    24901 cagactcaga gaatcggcca tcacctgttt actgagcaaa cctgccagtt gctccacttt
    24961 gcgcaggtta tgaaaacgta tcctgaccgg ctgctgcaag cccagacgca gaatttgcaa
    25021 agctctttta ttattcatac ccgcttcgag cacataatga ccactgtgca tctgtcgggg
    25081 ataatcgtaa tagtcggcgg cttttttgaa agaacgctca tccctcaaca ggttcttatc
    25141 ttttagctgc tgcaagaggc tgtcaaaatc gtcttcttcg tagagataaa tatgccggct
    25201 ttgtccgtca agcacattcg gacgataaaa gacaccataa atccagacta taatcccaac
    25261 agccagcaaa agcaacaaac ccgaagccgt caacaacaat cgtactttta ttttcacctc
    25321 tttctgtcat tacgctcccg agggagcatt tacactgttt ttttaataaa tcgcggcaga
    25381 aaatttgaat aaaataaaac tcttcctatc tttgcgccgc gttaaataaa ccaccatcga
    25441 attctcattt gtaattcaaa accgggctta ttatcgcgtt aataagataa ggaaggatgg
    25501 gtgagtggct gaaaccaaca gtttgctaaa ctgtcgtact cgtgagggta ccgggggttc
    25561 gaatccccct tcttccgcca actttgcaag gctgtttctc aaaagagacg gccttgtttt
    25621 ttctccaaga tctccccgct tgttttctct tatttcttcc attagaagtc cggatgtctt
    25681 cccggcctgt cgcccggtca tttcttatct cattcacttc cgccgggctt ttctcgggtt
    25741 taactttcca atcaaaaaat cccggcgctc tgctcaaaac ttcaggcgca aagataataa
    25801 tattcc
//
LOCUS       DAOSTD010000022        25769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_2100_length_25769_cov_16.469044, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000022 DAOSTD010000000
VERSION     DAOSTD010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25769
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2100_length_25769_cov_16.469044"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..597)
                     /gene="cas6"
                     /locus_tag="PLI37_02645"
     CDS             complement(40..597)
                     /gene="cas6"
                     /locus_tag="PLI37_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOQ57951.1"
                     /translation="MRFKLNLNLDPLPYGNTIPVNYQYELSGWIYRTLASGDQAYSTW
                     LHENGFTQNRKQFRLFTFSNLHIPKFQIKGDRLIILSDQACIYLSFLPERSTENFIKG
                     IFSSQSFVLGDRLSKMQCFVQSVELVEPPAYEPVMTFRTLSPMVLSGHLENGKPTYIS
                     PEDMRAAQLIYNNLCAKYQAFYRLQ"
     gene            complement(752..3052)
                     /locus_tag="PLI37_02650"
     CDS             complement(752..3052)
                     /locus_tag="PLI37_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOQ57952.1"
                     /translation="MDILQATNPEEEKIFEQFQLLLNDYLNSNHRKKVDLITKAFKFA
                     YNAHRGVKRRSGEPYIMHPLAVARICCAEIGLGSTSICSALLHDVIEDTEFTVEDIRN
                     NFNDRIASIVEGLTKISGGIFGENASTQTENFRRLLLTMSEDIRVILIKMADRLHNMR
                     TLSALSPSKQYKIAGETMYLYAPLAHRLGLNRIKTELEELSFKYEHPEAYEQITHKLA
                     ITAESRKALYYSFIEPVKHELDKLDLKYYIKERVKTAYSIWKKMTTKNIRFEEIYDLL
                     AVRIIFDSPDIEDEKEMLALEKKQCWDIYSTITNIYKPHPDRLRDWVNNPKANGYQAL
                     HTTVMGFTGDWIEVQIRSTRMHSIAERGFAAHWKYKLGEIEEDNELNHWILTIKEILE
                     SPEPNTLDVLDTIKMNLFSSEIFVFTPKGEIKTLAQGATALDFAFEVHSELGYRCIGA
                     KVNHQLVPMSHVLKSGDQVEILTSENQTPRYEWLKFVTTGKAHSKIKARFRRDNRETI
                     KKGEELLNEFFNKHHLTADADNVQVLLTNCNCINKEELLMKLGTGELTLNNAEAELFK
                     KDNQNLLVKYWKLTFGSKNSKQEKEKGKEKEKGKENKTIDLKSTHRLTEESVLNEYHL
                     SDCCQPIPGDAVLGFVAQNGIIHVHKRQCPIAMRLKSSYGDRIVSAEWATHKKLSFAV
                     EIEITGLDTVGMINQITRIVSDEFSVNINKLNFESKDGVFRGSMTVYVHDVESLNHLC
                     NKISKLKNIKSVTRVERQNKLMGVEE"
     gene            complement(3098..4498)
                     /locus_tag="PLI37_02655"
     CDS             complement(3098..4498)
                     /locus_tag="PLI37_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOQ57953.1"
                     /translation="MEQTEGLPGEAGNLPQQKRVNGKQTGKKLPDSLSNPEKTGQDNL
                     LAEEDAPAIEMNLDSIENSLFSEGLKILIEMDSSHYFDDPGLDPSFHFNANTDSLIYE
                     WQRQSLLIFDENCRPTLTDSSATPETTYIQRLMDLPNIIEMPYNRIVRRFIDLYLNER
                     RMQVEAMLGAGVYYFPLFEQVLDQNGLPIELKYLPVIESALNPKAVSRVGATGLWQFM
                     YATGKMYGLQINSLTDERRDPVKSTYAAVKYLKSLYSMFGDWNLAIAAYNCGPGNVNK
                     AIHRSGGKRNFWEIYYYLPRETRSYVPLFIAANYVMTYYSEHGICPAQNNFPPVIDTF
                     MIHQNMHFQQIAEVCQIPMQEIKLLNPHFRNEIAHGTATKAGILYLPMEYSPVFTELQ
                     DSIPRYKEQELLASRLVSVQSGQAGPTDAIVYRVKRGDNLSVIASRHNVSVQKLKNWN
                     NLKSNTLQIGQRLLIY"
     gene            4512..4661
                     /locus_tag="PLI37_02660"
     CDS             4512..4661
                     /locus_tag="PLI37_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57954.1"
                     /translation="MNIFLKNFPAAGDVEDSGDADDAEDSGDVDDSGDADNAGDADKP
                     KISSI"
     gene            complement(4697..5317)
                     /locus_tag="PLI37_02665"
     CDS             complement(4697..5317)
                     /locus_tag="PLI37_02665"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOQ57955.1"
                     /translation="MADSLSSVLADDLSRDGADGFLIADSLFLRPDMESFMNTDTAAR
                     ERQLFKPLPSKALWYAALCPGLGQFYNRKYWKLPIVASGVVGLAYAISWNGKYYVAYT
                     NAYRDISDNNPDTNSFLDLLPPGYSYTESQLRTVLKNRQQTFRRQRDLSIIGAVGFYL
                     ICILDAYVDAELFDFNVSPDLSIRLQPVRQAPGIDHAMEVSCLFRF"
     gene            complement(5430..6302)
                     /locus_tag="PLI37_02670"
     CDS             complement(5430..6302)
                     /locus_tag="PLI37_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOQ57956.1"
                     /translation="MAAKNRLGRGLDALISTEDIQTVGSSSISEVELELIRPNPEQPR
                     TDFEPEALEELAISIREIGIIQPITLRKMDDGYYQIISGERRVRASKSIGLKKIPAYI
                     KTAEDDSMMEMALIENIQREDLNAIEIALAYQKLAEVYKLTQEKLSERIGKKRTTISN
                     YIRLLKLPAEIQMGLKNKTIDMGHARALINVKSPALQLAIYEEILKQGLSVRKVEELV
                     RNEESGKSKNFKKPLPRKTSEEYLDLQKQLSQKLGTKVRFSINEKGKGSISIPFDSEK
                     KLEEILLSLDKLFN"
     gene            complement(6315..7079)
                     /locus_tag="PLI37_02675"
     CDS             complement(6315..7079)
                     /locus_tag="PLI37_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ57957.1"
                     /translation="MGKIIALANQKGGVGKTTTAINLAASLAVQEKKVLLVDADPQAN
                     ASSGLGIDIRQVGISIYECMIDGANPADGIQKTAIEYLDILPSHIDLVGAELEMINIE
                     NREKILSAVLQPLKSSYDYILIDCSPSLGLITVNCLTAADSVLIPVQCEYFALEGISK
                     LLNTIKIIKSRLNTGLEIEGFLLTMYDQRLRSARQVADEVRRHFQGLVFKTVIQRNVT
                     LSVAPSHGQPVMLYDADSRGAIDHIQLALEIIRKNK"
     gene            complement(7179..8312)
                     /gene="lpxB"
                     /locus_tag="PLI37_02680"
     CDS             complement(7179..8312)
                     /gene="lpxB"
                     /locus_tag="PLI37_02680"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOQ57958.1"
                     /translation="MKYYLIAGEASGDMHAANLMAALVEKDPKAEFRFLGGDKMAAIG
                     GTPLIHYKKMAYMGILPVILHARTLLSNMRICKKDVLSWQPDVLILIDYPGFNLNIAA
                     YIKKKQPGLPIHYYISPKLWAWKGYRIRNIRRDIDRLYSILPFEKTYFDRKNYRAFYV
                     GNPSLDSIEAFKNSKPDLKGWREKQQLTQKPIIALLAGSRRQEIKANLPLMLRVASAY
                     PDHQLVLAGAPGINKDFYAPYIKNYDCRLVYDETYSLLAQSKAALVVSGTATLETALF
                     GVPQVVCYALWIGGFLYKIGRKYLIRTPFISLVNLIAEKEVVVELVANEYNYEQLKNE
                     LNKILYDSAYIAEMQEAYGFMRKKLGEPGAAGRTASLIYNHLT"
     gene            complement(8333..9094)
                     /gene="surE"
                     /locus_tag="PLI37_02685"
     CDS             complement(8333..9094)
                     /gene="surE"
                     /locus_tag="PLI37_02685"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007898479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOQ57959.1"
                     /translation="MKPLFLISNDDGIHARGIRCLIDVLRPLGDMLVVAPETERSGMS
                     TAFTLREPLTCKPVSKEEGLIQYSSNGTPVDCIKLALNLLTDERKPDLIIAGINHGFN
                     GSIAIHYSGTMGAVLEGCMAGYPSIGFSIDDHREEANFEPSLPHIRNIVQAVLDNGLP
                     YGVCLNVNIPKHDEIKGTKICRQGKGRWVEEFIPCSHPDNAKRYWLTGKFLNLEENAG
                     DNDLTSMRQGYISVVPSCIDVTNHSLIGQLQTWEL"
     gene            9302..9757
                     /gene="rplM"
                     /locus_tag="PLI37_02690"
     CDS             9302..9757
                     /gene="rplM"
                     /locus_tag="PLI37_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOQ57960.1"
                     /translation="MDSLSYKTISVNKATAQKEWVVVDATDQVLGRLGTKVAKLLRGK
                     YKPSFTPHVDCGDNVIIINADKVRLTGSKWNDRIYLRHTGYPSGQRKFTPASLMAKDP
                     LSLYRKVVRGMLPKNRLGEAIINNLYVYAGNEHPHQAQKPKAIDLNSLK"
     gene            9761..10147
                     /gene="rpsI"
                     /locus_tag="PLI37_02695"
     CDS             9761..10147
                     /gene="rpsI"
                     /locus_tag="PLI37_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOQ57961.1"
                     /translation="MEAVNAIGRRKAAIARVFVTEGNGTININKKDLSEYFPSDILQY
                     VVKQPLKKLEVDGKYDINVSLKGGGFKGQAEALRLAISRALVKINPEDKPALKAEGFM
                     TRDSRIVERKKPGRPKARKKFQFSKR"
     gene            10283..11149
                     /gene="rpsB"
                     /locus_tag="PLI37_02700"
     CDS             10283..11149
                     /gene="rpsB"
                     /locus_tag="PLI37_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOQ57962.1"
                     /translation="MSRTNFDQLLEAGVHFGHLKRKWNPAMAPYIFMERNGIHIIDLH
                     KTITKLDEAAEAMKNFARSGKKILFVATKKQAKEIVAEKAASVNMPYVIERWPGGMLT
                     NFPTIRKAVKKMSTIDKMFTDGTYSNLSKRERLQIGRKRAKLEKNLGSIADLNRLPSA
                     LFVVDVMKEQIAVHEANRLGIPVFAMVDTNSDPSNIDFVIPANDDATKSIDIIVSTVC
                     AAIAEGLEERKIEKADADAAAAVAEEEEGNENVSRRERRPKTARRERIQKEDEEALKA
                     RVTSKFMKDDDE"
     gene            11207..12028
                     /gene="tsf"
                     /locus_tag="PLI37_02705"
     CDS             11207..12028
                     /gene="tsf"
                     /locus_tag="PLI37_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOQ57963.1"
                     /translation="MAVTMADISKLRSMTGAGMMDCKNALTEANNDMEKAIEIIRKRG
                     QAIAAKRSDREASEGCVLADCKDGFAAIVALKCETDFVAKNQDFIALTKSILDLALAT
                     KPESLEALQNSTMDGRTVADLVTERSGITGEKMELGYYECVKAASVMHYVHPGNKLAT
                     IVGFNKESEYQAARDVAMQIAAMNPVSLDRASVPAKIVQQELEIGREKARQEGKPENI
                     LDKIAQGRLNKFYQESTLMEQAFIKDGKISVAQYLKSVDEDLVPVDFKRVTLNQE"
     gene            complement(12155..12553)
                     /locus_tag="PLI37_02710"
     CDS             complement(12155..12553)
                     /locus_tag="PLI37_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOQ57964.1"
                     /translation="MPAQDWKERLAALAKISKEQSENTETPRFSSASNPKQEHQNPAS
                     SPAPVKKGQLCIRFEKRNGKPATIISEFKGSQQELMELARQLKSTLGIGGSAKDDEIL
                     LQGDLRDKIAPYLQKLGYSLKGDYKKNGSR"
     gene            12700..13341
                     /locus_tag="PLI37_02715"
     CDS             12700..13341
                     /locus_tag="PLI37_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOQ57965.1"
                     /translation="MNEIKKGSTVPEPTLRRLPWYLAFLKMAAARGEIRISSTQISRA
                     INVSAPQIAKDLSFLEISGKTRIGYDVQSLISVLEEYLGFTKIRKAFLFGVGNLGAAL
                     LHDKGLSQFGLEILAGFDVNPRVVGTTIQGVPVYHIKDYPGIQAKIDASIGILTVPVR
                     EAQKVAEEIIQGGIRAIWNFTPFRIHVPEDIVVQNTSIYAHLAVMYNRMEAKL"
     gene            13348..13959
                     /locus_tag="PLI37_02720"
     CDS             13348..13959
                     /locus_tag="PLI37_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOQ57966.1"
                     /translation="MKIVAVGMNYLPHIQELKSEIPEEPVLFIKPDSALCSDKQPFFL
                     PDFSQEIHYEVELVVKISRLGKNIAEKFAHRYYDEITLGLDLTARDLQRNLRACGLPW
                     EISKGFDQSAVVGHFIPKNRFENIQQLHFRLDLNGKTVQQGSTAEMLFGVDRIIAYAS
                     RFFTLKTGDLFFTGTPCGVGPVHKEDHLEAWLEGEKMLHLRVK"
     gene            14013..>15558
                     /locus_tag="PLI37_02725"
     CDS             14013..>15558
                     /locus_tag="PLI37_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57967.1"
                     /translation="MKLYKAIWSLILSCIFTASYAHSLDWYYSLSAQGGRLWSFEGAV
                     QIEEGGLPLYSETFRPAADILRPELRFPVWEEIKGKDLQAYKSLFDKFPDSLELHINK
                     GIQRKEAVWDLYFYPFLHKNGRYYRLLSFEWAYIREATAGLLNEDGQEDTPHFVSSLQ
                     EQPGAESPGQRYAESSRLSSGKFIKISVENTGIYKLSFDDLRKMGIDPAKVQLYGYGG
                     QLLPEDFTQPYIDDLPELSFYIDKEQGYLLFYGLGPLSWNYEASKGLFTRLNNHYSTK
                     GYYFVGERSEGTKPIDELPVPDREPDSEVRAYTAYKLHERDLYSLGGTGRECYGEDLM
                     SNPDLNLSFHFDDILSGEQASIVVETAARSTSPGSTRLYVDNSFMGSMNYSAVSSSDS
                     YTYARGSSLNKTFLPQGNTVAVRLQYNNNGVIPKNAHLNYVAINARCRLVLNSPSLAF
                     RDPKSLGAGKVSRFCLESADAFTRVFEISKTGSMKQVAGELKGTEYQFKVTTDSLREF
                     VAVNLKG"
     assembly_gap    15559..15658
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15659..17989
                     /gene="porU"
                     /locus_tag="PLI37_02730"
     CDS             <15659..17989
                     /gene="porU"
                     /locus_tag="PLI37_02730"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOQ57968.1"
                     /translation="FKVTTDSLREFVAVNLKGNFPKPVVEGQVPNQNLHGLPAPDFVI
                     ITHPDFIAEARELAQAHRDHDRMSVQVVTTEQVYNEFSSGTPDATAYRRFLKMFYDRA
                     TTAEDIPQSLLLFGDGVYDNRLVTAAFSSGFSNPNKILTFQSKESLDGTSSYVSDDYF
                     GFLDDNEGVNLPYDKMDIGIGRFPVRSKVEARIVVDKTLSYINNRDKGSWKNQLCFIG
                     DDGDNNTHVSHADKLAESVKSNNPNFITHKIYVDAYQRETSSAGTTVPDANKRLNELL
                     GSGLLLLNYSGHGSTTTWASEQLFTIGEIKGMQNKRLPLWVTATCDFCRYDDAVTSGG
                     EYVFLNEQGGGIALFTTSRVVYSGPNYTINKSFIDHVFATKNGRRLSLGEVMCLTKQS
                     ESLKHDRNKLNFALIGDPALKLSYPEYKLEITHVNGRELQAGVYDTLSALSTAEFGGR
                     VLRYDGSFADDFDGLVYPVVYDAEDLVETLGNSGNDVFKYYDRSKVLFAGKEQISKGR
                     FTFRFIVPKDLSYSYETGMINLYAYNTRGDIEAQGVFKQFLLGGTNDAVLPDSTGPVI
                     RLFLNDTSFVDGGKVNTKPSLIALLQDESGINMSGMNFGHDLVLTIDDKDRHILNNYY
                     ESDLGSGNSGRIIYKLPELETGRHSLSLRAWDLQNNSSVASIDFLVVDDLSPQLRDLN
                     FYQNSEKAWFVFRHDRPASWMQVEFRVYDVWGRLQWRKQVNMMNPDNSSQELEWNYVG
                     ENGRRVENGVYICRLFVVSDDSEQAVTAKKIMVGLQ"
     gene            18037..19158
                     /gene="porV"
                     /locus_tag="PLI37_02735"
     CDS             18037..19158
                     /gene="porV"
                     /locus_tag="PLI37_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOQ57969.1"
                     /translation="MVKYVSPVGAVVTAALLFFSIDLSARDYNPIVTSVPSLSIAPDA
                     HATGLGDVGAATEPNVNSQYWNPSKYAQMENSAGFALSFTPWLSQLVSDINLTNLTGY
                     MKIGDLQSLSASLRYFSLGEVILRQSLGDQGYAIQPYEMALDVAYSRMLSQYYSMSVA
                     LRYIYSDLMSSDDPAGNAFAADISGFYRRPVYFGREQGFWAFGFDVSNIGTKISYDAN
                     NTSYFIPTNLRLGFSVKYPFNSYNAITVSGDVNKLLVPTPQDSTDFATDISSMVGIFK
                     SFTDAPGGLSEELSEINWGIGLEYAYDGQFFVRMGYHFEDKNKGNRKYFTFGAGFKMS
                     MFSIDASYLLSQAQTSPLDQTLRFTLGFDIEGIRNLLGR"
     gene            19160..19639
                     /gene="ispF"
                     /locus_tag="PLI37_02740"
     CDS             19160..19639
                     /gene="ispF"
                     /locus_tag="PLI37_02740"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOQ57970.1"
                     /translation="MQLRVGQGYDVHRLVAGRELWLGGILIPFEKGLMGHSDADVLIH
                     AVCDALLGAAGLGDIGFHFPDNAAEYKNIDSKILFKRTIDLLAEKQWEVLNIDATLLA
                     ERPKINSYIPQMQACLAGLAQVDKEKISIKATTSEKMGFVGREEGMAAMAVALIGRK"
     gene            complement(19623..20531)
                     /locus_tag="PLI37_02745"
     CDS             complement(19623..20531)
                     /locus_tag="PLI37_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57971.1"
                     /translation="MPEKFCSCCFVLSLLLIAPSIRAQPIPDWWKQLHQYDEKLDWAD
                     YMTYTPAFFGPNALPVPELYDGIIGDRHQLEFSGNLFWGPGEQTQSLATRLSYVFVPG
                     RLSLSAWGVLGEHYRTSREIRDQRASLIEEAEELIFIGDFYLATQFRILAENRYVPEI
                     LGEIVLKTASSDRSRGARFFDTPGYYFDLSLAKDLYHSQAGLLKNIRLVAMGGFLCYQ
                     MNNNYQNDAPIYVAKLKLDFGALGWENGMGGYYGWTGKGDRPLVLRSRLDFETGKNKA
                     FVQYQAGLNDYIAHCLQAGWVFTFGR"
     gene            complement(20596..21138)
                     /locus_tag="PLI37_02750"
     CDS             complement(20596..21138)
                     /locus_tag="PLI37_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57972.1"
                     /translation="MKKILFSLTALLIACTSFAQDNDPFRVGENSGSLKVQEESNQPF
                     ETTVQRKKVQTSSKEAPKEEEKPAVTVNNPSKDIKVEFVSCIGNRAAQTFELTIKVTN
                     LQVNQQIYLNSLLAYDNQGDENYAYGKNWEALTDVTVRQVFAFRNKVLPAKVDKLSVI
                     KLPFVKFGSVEFRNVTIDWR"
     gene            complement(21143..22066)
                     /locus_tag="PLI37_02755"
     CDS             complement(21143..22066)
                     /locus_tag="PLI37_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57973.1"
                     /translation="MKNIILTILLLCPLLGTAQKVEEKSHKSKPAWVNGAEMGYLVVS
                     AEASDIESAKTMILGQLKAQVAGSVASRVLSESTIRTRETSINDAQRQRSEEYESYIN
                     TKAANIPFINEISLSKASDYYWEKIYDKKSKSSIYQYHVKYPFSDLELKRMVEAFLAH
                     EAELDARIKQYADDLNTIPSVEFIDKTLNELRAFLQEFTKDDPRYAQVEQLSNNYRRA
                     YKNIIVEEVSNKPGEVSVRLVLNRRNITTSQQARISSNCADQFNPVYQGDLLVVKFND
                     FNCYEDDSNYIDIRFKFGNDFVSKKIYFKKN"
     gene            complement(22079..22771)
                     /locus_tag="PLI37_02760"
     CDS             complement(22079..22771)
                     /locus_tag="PLI37_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57974.1"
                     /translation="MKKICTLLLGLSLSFMLSFAQEINEASRIAIKVEIPQNIDLKTD
                     ASNILFNRLNQAVALNGLGAASGKFLLVPRVTLLSKDVTTTIPQQFVVEMEVFLFIVD
                     VTQKTILQQESVTLKGIAENENKAVLKAISSLQARNPRLKNLIVRGKDKIVAYYSTEC
                     DVLLKKAESLIARNMYYDAIVELESVPLLNNNVSCFEKSLRMLEQIDQSIRLEAKEKM
                     EQSKHNLDWLTE"
     gene            complement(22773..23750)
                     /locus_tag="PLI37_02765"
     CDS             complement(22773..23750)
                     /locus_tag="PLI37_02765"
                     /inference="COORDINATES: protein motif:HMM:NF040342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HOQ57975.1"
                     /translation="MRNLLLCIALAATILNVSGQAKKPILMVVPSDSYCDRNAFVQEF
                     TDEAGNTRSISDYNKAFKSAESEELRLVISELSKIMAERGFPLKDLEQTMKSIAQSNV
                     ELSLLQSSSSGSAIRESPIDVLKRTAQADIIMDIDFSVKIRGPQRFITYNLRAVDAYT
                     NKVITAVAGDGKPSSSASVGLLLEEAVLNYMDDYTSALQNHFDDIFTNGREVVVVLRV
                     WDNADVDFETDFEYMGETGMLGDIMDVWMDDNTVNSRFSRVSATENTIRYEQVRIPLF
                     KMSFGRERAIDARGFINGLSTMLKAEPFNIQSKIYERGLGEVWLILGEK"
     gene            complement(23769..24212)
                     /locus_tag="PLI37_02770"
     CDS             complement(23769..24212)
                     /locus_tag="PLI37_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57976.1"
                     /translation="MFKSAASYEVQVLGVGQDGTKVFRIWAYDKTVEAAINKAKRDAV
                     AACLFSGLPGSGHTNQTPAICKLSDEETHEAFFQEFFAYPDKKGNGGAYLRFVNRTTD
                     EPPSGKYRVQMKGGYKIAIDVQVMYNNLRSYMEEKGIVKKLGHGF"
     gene            complement(24342..>25769)
                     /locus_tag="PLI37_02775"
     CDS             complement(24342..>25769)
                     /locus_tag="PLI37_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57977.1"
                     /translation="SRAHFFPEGSIWPKAPDRTELISSMLNEQRAGQQIVAYIFNRQP
                     DGNMNLDRVFERGEYSAHDVDMRLSGTLKVETVRDAGSGLIGRSYLMVYDVTNLRTEY
                     SGKDDNRELYWKATAALYLYKLNWDEQDLNTFYDKYWLDENTPAEERQAKKAAFEAWN
                     IPVELVERVNLGDKSVSTGIEANKRRGVLSNKKITDEQRLANAFNELQEQIYSDLNYK
                     IEKQHEDFIVTTPITSLRPIRAKIGTKEDVRHGLRFYAYGFKGNSDGGIKLVRKGILR
                     ATKKVADNRTVATGESPESEFTKIAGYGRVVEGLTLVEKKDLNLSLGVGFNSIPQGGP
                     VNSGFHADLSYENHTGRRFCSYTSLAFNLDGSAMNLILSYGYGLRIPNFEIYPFAGFG
                     MDTYSFDGSDAYEKADLRAYVLQGGLRAQVNIYYPVYLFWNVSYNAVFGKGNIYQAYE
                     GVSNNMGNFRDMNGSQTALGIRYCF"
BASE COUNT         6279 a         5739 c         6465 g         7186 t
ORIGIN      
        1 ggatctcaca acccacgtac tacaaatgga gcaaagagtt cattgaagcc ggtaaaaggc
       61 ttgatattta gcacatagat tgttgtagat caattgagct gcccgcatat cttcgggcga
      121 aatataagtc ggcttgccgt tttccagatg tcccgataga accatgggcg aaagagttcg
      181 aaaggtcatg accggttcgt aagccggtgg ttcgacaagt tctacgcttt gcacaaaaca
      241 ttgcattttg ctcagacggt ctccaagtac aaaagactgg gaagagaaaa tgcctttgat
      301 aaaattttca gtggagcgtt cagggagaaa ggacaggtat atacaagcct gatcggataa
      361 gataatcaga cgatcgccct ttatctgaaa ttttggaatg tgcagatttg aaaaagtgaa
      421 caaccggaat tgctttcggt tctgggtaaa accattttcg tggagccagg ttgaataagc
      481 ctgatccccg ctagccaacg tacgataaat ccatccggat agttcgtatt ggtagttgac
      541 agggattgtg tttccgtagg gtaatggatc taagtttaaa tttaatttaa atctcattat
      601 tttgattaaa tttcccgcaa gttaaataat ttatatttga tgcgctttat ttttctgtgc
      661 ttattttgga gtcatatcgt tatttttata ttaagtttaa atttcggggc acaaaaatct
      721 ccccaaaacc tcccaccacc ccttcaaaaa ctcactcctc cacccccatc aacttatttt
      781 ggcgttctac gcgggtgacg gatttgatgt ttttgagttt ggagattttg ttgcagaggt
      841 ggttaaggct ttctacgtcg tggacgtaaa cggtcatgga gcctcggaat acgccgtctt
      901 tggattcgaa gttgagtttg ttgatgttta ccgaaaattc gtcggagacg atgcgggtga
      961 tctgattgat catgcctacg gtgtcgaggc cggtaatttc tatttcgacg gcgaaagata
     1021 attttttgtg tgtggcccat tcggcagaaa cgatgcggtc gccgtagctg cttttcaggc
     1081 gcatggcaat ggggcattgc cgtttatgta cgtggataat gccgttttgg gctacgaaac
     1141 cgagtacggc gtctcccggt atgggctgac agcaatccga caggtggtat tcgttcagaa
     1201 cagattcttc ggtaagccgg tgtgtcgatt ttaagtcgat ggttttgttt tcttttccct
     1261 tttctttttc tttccctttt tccttttcct gcttgctgtt cttcgatccg aaggtgagtt
     1321 tccagtattt gaccagcagg ttctgattgt cttttttgaa gagttcggct tcggcgttgt
     1381 ttagtgtcaa ttcgccggta cccagcttca tcaaaagttc ttccttatta atgcaattgc
     1441 agttggtcag caatacctga acattgtcgg catcggccgt taagtgatgc ttgttgaaaa
     1501 attcattgag cagttcttca ccttttttta ttgtctcgcg gttgtctctg cgaaaacggg
     1561 ctttaatttt ggaatgagcc ttgccggtgg taacaaactt cagccattcg tagcggggag
     1621 tttggttttc ggaggtgaga atttccacct ggtcgccgct tttgagcaca tggctcatgg
     1681 gtaccaattg atggttgact ttggcgccga tgcaacggta gcccaactcg ctgtgcacct
     1741 cgaaggcaaa atccagggcg gtagctccct gagccagggt tttgatctct cccttggggg
     1801 taaatacgaa gatttcggac gaaaacaggt tcatcttgat ggtgtccagc acatcgaggg
     1861 tgttgggctc ggggctttcc aaaatctcct tgatggtgag tatccagtgg ttgagctcgt
     1921 tgtcttcttc gatttcgccc agtttgtatt tccagtgggc ggcaaaacct ctttcggcaa
     1981 tgctgtgcat tctggtactg cggatctgga cttcgatcca atcgccggta aagcccatca
     2041 ccgtggtgtg cagagcttga taaccattgg cttttgggtt gtttacccag tcgcgtaaac
     2101 ggtcagggtg aggcttgtaa atattggtga tggtggaata tatatcccag cattgtttct
     2161 tttcgagggc aagcatctct ttttcgtcct cgatatcggg cgaatcgaaa atgatacgga
     2221 cagccaaaag gtcgtagatt tcttcgaacc ggatgttttt ggtggtcatt ttcttccaga
     2281 tggagtaggc tgtttttact ctttccttga tgtagtattt caagtccagt ttatccagtt
     2341 cgtgcttaac gggttctata aaggaatagt atagtgcctt tcgcgattcg gccgtgatgg
     2401 ccagtttatg tgtgatttgt tcgtaagctt ccggatgctc gtatttgaaa ctcagttcct
     2461 cgagttcggt tttgatccgg ttcaatccca gacggtgggc cagcggagca tacaaataca
     2521 tggtttcgcc ggcaattttg tattgtttgc ttggcgacag ggcgcttagg gtcctcatgt
     2581 tgtgcaggcg gtcggccatt tttatcagga ttacccggat gtcttcggac atggtcagga
     2641 gcaggcgcct gaaattttcg gtttgagtgg aagcgttctc cccaaagatt cctccggaga
     2701 tctttgtcaa accttccact atggaggcaa ttctgtcgtt gaagttgttt cggatgtctt
     2761 cgacggtaaa ctcggtgtct tctatcacgt cgtgcaaaag agcggaacag atagaggtgg
     2821 aacccagacc gatttcggcg caacagatgc gggctacggc cagaggatgc attatataag
     2881 gctctcccga gcggcgtttg acacctctat gggcattgta agcaaactta aatgccttgg
     2941 taatcaaatc cacttttttg cgatggtttg aattcaggta atcgttgagc aaaagctgaa
     3001 attgctcaaa gattttttct tcttcaggat ttgttgcctg taaaatatcc ataatcggcc
     3061 tctgtgcaga gttttatgcg aaggtactat ttaatcgtta ataaataagc agtcgttgcc
     3121 caatttgcaa cgtattactc ttaaggttat tccagttttt cagcttttga acacttacgt
     3181 tgtgtcttga tgcaattacc gagaggttgt cgccccgttt taccctgtaa acaatggcat
     3241 cggttggccc cgcttgtccc gattgaaccg aaaccagcct cgaagccagc aattcctgtt
     3301 ctttgtaacg gggaatgctg tcttgcaatt ctgtaaagac gggcgaatat tccatgggca
     3361 gatagagtat gccggccttg gtggcggtgc cgtgagcgat ctcgtttctg aaatggggat
     3421 tcaataattt tatttcctgc atcggtatct ggcagacttc ggcaatttgc tggaagtgca
     3481 tgttttgatg gatcataaaa gtatcaatca ccgggggaaa attgttttgg gccggacaaa
     3541 taccgtgttc ggaataatag gtcatcacat aattggcggc aatgaacagg ggcacatagc
     3601 tgcgggtttc ccggggtagg taatagtaga tttcccaaaa attgcgtttc ccgcctgagc
     3661 ggtgtatggc tttgttcaca ttgcccgggc cgcagttgta ggctgctatg gccaggttcc
     3721 agtcgccaaa catggagtag aggctcttca ggtatttgac ggcggcataa gtcgatttaa
     3781 ccggatcccg cctttcgtcg gtaaggctgt tgatctggag tccgtacatc ttgccggtgg
     3841 cgtacataaa ttgccataaa ccggtggcgc cgactctgga aacagccttg ggattcagcg
     3901 ccgattcaat gacgggcaaa tatttgagtt ctatgggcaa accattttgg tcgagtacct
     3961 gttcaaacag tgggaaataa tagactcccg ctcccagcat ggcttctacc tgcatcctcc
     4021 tttcgttcag atagaggtcg atgaaacgtc ttactattct gttgtagggc atttcgatga
     4081 tgttgggtaa atccataagc cgttgaatgt aggtcgtttc gggtgtggca gagctgtctg
     4141 taagagtggg tcggcagttt tcgtcaaaaa tcagcaggga ctgcctttgc cattcgtaaa
     4201 taaggctgtc ggtgttggca ttgaaatgaa aagagggatc caggccggga tcgtcgaaat
     4261 aatggctgga atccatttcg atcagtatct tcagcccttc gctgaagagg ctgttttcta
     4321 ttgaatcgag attcatttca atggccggtg cgtcctcttc ggccaacagg ttgtcttgcc
     4381 cggttttctc cgggttggac aagctgtccg gcagtttttt gccggtctgt ttgccgttca
     4441 ctcttttttg ctgcggcaga ttgcctgctt ccccggggag gccctcggtt tgctccattg
     4501 agcttttttt agtgaatatc ttcttaaaaa attttccggc tgcgggcgat gtagaagatt
     4561 caggcgatgc agatgatgca gaagattcag gcgacgtaga tgattcaggc gacgcggata
     4621 atgcgggcga cgcagacaag cctaaaataa gcagtatata gatgattctt ttcataatcg
     4681 gtttttctcg ttgttgttaa aacctgaata aacaggatac ttccattgcg tgatctattc
     4741 cgggggcctg ccggacaggt tgcaggcgga tactcaggtc gggactgacg ttgaaatcga
     4801 acagctcggc atcgacgtag gcatccagga tgcaaattaa ataaaagccc acggctccga
     4861 tgatgctcaa atcgcgttgc ctgcgaaagg tctgttgacg atttttgagc accgttcgca
     4921 attgcgactc ggtataggaa tagcccggcg gcagtaagtc caaaaaactg ttggtgtcgg
     4981 ggttgttgtc gctgatgtct ctgtaggcgt tggtataggc aacataatat tttccgttcc
     5041 agcttatggc gtaagcaagc cccacgaccc cgctcgctac aatcggcagt ttccagtatt
     5101 tccggttgta aaactgtccc aaacccggac aaagggcggc gtaccacaga gctttggagg
     5161 gaaggggctt gaacaattgc cgttcccggg cggcagtatc ggtattcata aagctttcca
     5221 tgtcgggcct caaaaagagg ctgtctgcaa taaggaagcc gtctgctccg tcgcgggaga
     5281 gatcgtcggc aagtaccgaa gaaagactgt ccgccacgag gaccgtgctg tcttgggttg
     5341 ccgaccggca ggccagacag caaaaaagca gtattggtat gattttggcg aaatagccca
     5401 tcttcttatc gatccggttc aggtcttgtt tagttaaaca atttgtcgag actgagcagt
     5461 atttcttcga gctttttttc cgagtcaaag ggaatgctga tgctgccttt gcccttttcg
     5521 tttatactga agcgcacttt ggtgcccagt ttctgtgaaa gctgtttttg taagtccagg
     5581 tattcttcgg aagtcttgcg gggaaggggc tttttgaagt ttttcgattt gccgctttct
     5641 tcgttgcgaa ccaattcttc gactttgcgc accgataagc cttgtttcag aatttcttcg
     5701 taaatggcca gctgcagagc ggggcttttt acattgatca gcgcccgggc atgtcccata
     5761 tcgatggttt tgtttttgag ccccatctga atttcggccg gcaacttgag caaacgtatg
     5821 taattgctga tggtggttct ttttttgcct atgcgctcac tgagtttttc ctgggtcagt
     5881 ttgtagactt cggccaattt ttggtaagcc aaagcgattt cgatggcatt gaggtcttcg
     5941 cgctgtatat tctcgatcag agccatttcc atcatcgagt cgtcttcggc ggttttgatg
     6001 taggccggga tttttttaag tcctatggat ttggaagcgc gtacacggcg ttctcccgag
     6061 atgatctggt aatagccatc gtccattttg cgcagggtga tgggttggat aataccgatt
     6121 tcccggatgg aaatggccag ttcctcgagg gcttcgggct caaaatcggt tctgggttgt
     6181 tcgggattgg gcctgataag ttccagctca acctcgctga tggacgatga gcctacggtt
     6241 tgtatgtctt cggtgctgat cagggcatcc aatcctcttc ccagtctgtt ttttgctgcc
     6301 atagcgtaag gggcttactt gtttttgcga ataatttcca gagccagttg aatgtggtcg
     6361 atggctcctc tggagtcggc atcgtagagc atcaccggct gtccgtggct cggggccaca
     6421 ctcagggtaa cgttgcgttg aatgactgtt ttgaatacca ggccttgaaa atgccggcgg
     6481 acctcatcgg ccacctgtct ggccgagcgc aggcgttggt cgtacatggt cagcaaaaag
     6541 ccttcaattt ccagtccggt atttagccgg cttttgatga tcttgatggt gttgagcaac
     6601 ttgctgatac cctccagggc gaagtattcg cattgtacgg gaatcaacac agagtcggca
     6661 gccgtcaggc agttgacggt gatgagtccc agagagggag aacagtcaat cagaatataa
     6721 tcgtaggaac tttttagagg ctgcaacaca gcagacaaaa tcttctcccg gttttcgatg
     6781 ttgatcattt ccagttcagc tcctaccagg tcgatgtgag agggcaaaat atccaggtat
     6841 tctatggcgg ttttttgtat gccatcggcg ggatttgctc cgtcgatcat gcattcgtaa
     6901 atgctgatgc cgacttgcct gatgtctatg cccagtcccg acgaagcgtt ggcctgcggg
     6961 tctgcatcga ccagcaagac cttcttttcc tgtacagcca gcgaggccgc cagattgatg
     7021 gcggtggtgg tcttacctac accgcctttt tgattggcta atgctataat tttccccata
     7081 aaccaacgca gatttttttc aagctacaaa gatagaaaaa ataattgtgt ctgtcttttt
     7141 ctttgtgcaa aatctaggtc gattcctttt agcttacttt aggtcaagtg gttgtaaatc
     7201 aggcttgctg ttcggccggc agctcccggt tcgcctagtt ttttacgcat aaagccgtaa
     7261 gcctcttgca tttccgcgat ataggccgaa tcgtacaaaa ttttattcaa ttcgtttttg
     7321 agctgctcgt agttgtattc gttggccacc aattccacca ccacctcttt ttcggcgatc
     7381 aggttgacca gcgaaataaa gggagtgcga atcaggtatt tacgtcctat tttgtacaaa
     7441 aaacctccaa tccacagggc gtagcaaacc acttgcggca ctccgaacaa ggctgtttcc
     7501 agggtggccg tgcccgaaac taccagggct gccttgcttt gggcgagcaa agaataagtt
     7561 tcgtcgtaga ccagccggca gtcgtagttt tttatgtagg gggcgtaaaa atctttgttt
     7621 atgcccggag ctccggccag tacaagttga tgatcgggat aggcggaggc caccctgagc
     7681 atcagaggca ggttggcttt gatttcctgg cgtcggctgc ctgccagcag agcaataatg
     7741 ggtttttgtg tcagttgctg cttttcgcgc cagcccttta aatcgggctt gctgtttttg
     7801 aaggcttcta tactgtccag actgggatta cccacataaa aggcccggta atttttccgg
     7861 tcgaaatagg ttttttcgaa agggagtatg gaatacagtc ggtcgatgtc gcggcggatg
     7921 ttgcgtatgc ggtagccttt ccaggcccag agtttgggcg agatataata atgtatgggg
     7981 aggccgggtt gctttttttt gatgtaagcg gcaatattca ggttgaagcc cggataatcg
     8041 atcagtatga gtacatcggg ctgccagctc aggacgtctt ttttgcagat tctcatattg
     8101 ctcagcagag tccgtgcgtg aagtattacc ggcaggatgc ccatataggc catctttttg
     8161 taatgaatca agggcgttcc tcctatggcg gccattttat ctcctcccaa aaagcgaaat
     8221 tcggccttgg ggtctttttc gactaaggct gccatcaggt tggcggcgtg catgtcgccc
     8281 gaagcttcac cggcaattag atagtacttc atcagttcaa aggctcaata actcaaagct
     8341 cccaggtttg cagttgaccg ataagagaat ggttggttac gtcgatgcat gagggcacca
     8401 ccgaaatata accctggcgc atggacgtca ggtcgttgtc gccggcattt tcttcaaggt
     8461 tgagaaattt gccggtcagc cagtaacgtt tggcattgtc gggatgcgaa caggggataa
     8521 attcttcgac ccagcgtccc ttgccctggc ggcatatctt ggttcctttg atttcgtcgt
     8581 gctttggaat gttcacgttg aggcagacac cgtaaggcag gccattgtcg agcactgcct
     8641 gcacaatgtt gcgaatgtgg ggcagggaag gctcaaaatt agcctcctct ctgtggtcgt
     8701 ctatggaaaa gcctatcgac gggtagccgg ccatgcagcc ttcgagtacg gctcccatgg
     8761 tcccggaata atggatggct atggagccgt tgaaaccgtg gttgatgccg gcaatgatca
     8821 gatcgggttt acgctcgtcg gtcagcagat tcagtgccag tttgatgcaa tctaccggtg
     8881 tgccgttgct cgaatattgg atcaggcctt cttcttttga taccggtttg caggtgagag
     8941 gctctcttag ggtaaaagcc gtagacatac cggatcgttc ggtttcgggg gccactacca
     9001 acatatctcc caaaggtctc aatacatcaa taaggcagcg tattccacga gcgtgaatac
     9061 catcatcgtt cgaaatcaaa aacaaaggtt tcataggcgt aattcaaaat aacaggcgca
     9121 aaggtagtca aaaaaaaacg tagattcttt tgtgagatat gaaaattcgc cgtacttttg
     9181 caggccaatt ctctatggtt tttttaataa gattttagtt cataaggaat taagctcgaa
     9241 agccttgaga tttgtttatt ttatacgata attaattaac aattaaaatc caaaaaaagc
     9301 agtggacagt ttgagttaca agaccatttc tgttaacaag gcaacggctc aaaaagaatg
     9361 ggttgtagtt gatgcaaccg atcaagtatt gggacgtttg gggacgaagg ttgccaagtt
     9421 gctgagaggt aagtacaaac caagctttac accccatgtg gattgtggtg acaatgtcat
     9481 cattatcaat gcggataaag tcaggcttac cggttcgaaa tggaatgatc gcatttattt
     9541 gcgccacacg ggatatccga gtgggcaaag aaaatttact ccggcatcgt taatggccaa
     9601 ggatccatta agtctatacc ggaaagtggt aaggggtatg cttcccaaaa accgtttggg
     9661 tgaagctatt atcaacaatt tgtatgtgta tgcaggaaac gaacatcctc atcaggcaca
     9721 aaagccaaag gcaatcgatt taaactcact taaataaatt atggaagcag taaatgcaat
     9781 aggaagaaga aaggcagcga ttgcccgtgt atttgttact gaaggaaacg gaaccattaa
     9841 tatcaacaaa aaggatctgt cggagtattt tccttccgat attcttcagt atgtggttaa
     9901 acagccgcta aaaaaactgg aagttgacgg gaaatacgat atcaacgtaa gccttaaagg
     9961 aggcggtttc aaaggacaag ccgaggcttt gcgcctggcc atttcccgcg cccttgttaa
    10021 aatcaatccc gaagataagc ctgctttgaa ggccgaaggg tttatgaccc gcgattcccg
    10081 cattgtggaa cgcaaaaaac cgggtcgtcc caaagcaagg aagaaattcc agttcagcaa
    10141 gcgttaatat tttgatacca ctgctcgata aatgaaaaag tagcgtttag tatctaaatg
    10201 gtcaggactt ccggctcagg ccgagaacta ccggacgatt gaatgagccc caaaagaatg
    10261 taaacgaaat taataaacca tcatgtcaag aactaatttt gatcaattac tggaagccgg
    10321 agtgcatttc ggacacctta aaagaaaatg gaaccccgcc atggctccgt acatatttat
    10381 ggaacgtaac ggcatccaca ttatagacct gcacaaaaca attaccaaac tggacgaggc
    10441 tgccgaggcg atgaaaaatt ttgcccgttc gggaaagaaa atactctttg ttgccaccaa
    10501 aaaacaggcc aaagaaatcg ttgccgaaaa ggctgcatcc gtcaatatgc cctatgttat
    10561 agagcgttgg cccggaggaa tgctcaccaa tttccccacc atccgcaaag ctgtaaagaa
    10621 aatgtctact atcgacaaaa tgtttacaga cggtacgtat agcaatttgt ccaaaaggga
    10681 gcgtttacag attggtcgca aaagagccaa gttggaaaag aatctgggca gtattgccga
    10741 tttgaacaga ttgccttcgg ctttgtttgt ggtcgatgtt atgaaagaac aaattgccgt
    10801 tcacgaggcc aatcgtttgg gtatccccgt ttttgccatg gtcgatacca attccgatcc
    10861 ttccaacatc gatttcgtta ttcccgccaa cgacgacgcc accaaatcca tcgatattat
    10921 agtatcgacg gtttgtgccg ctattgccga aggtctggaa gaacgcaaga ttgaaaaagc
    10981 cgatgccgat gctgctgctg ccgttgccga agaagaagag ggtaacgaaa acgtaagccg
    11041 cagagagcgc cgtcccaaga cagcacgcag ggaacgtatc caaaaagaag acgaggaagc
    11101 actgaaagca agggtaacct cgaagtttat gaaagacgac gacgaatagt cggaacatgc
    11161 cggaatggaa ccggtaacga agattgttta taaaaaaaga agaattatgg cagtaactat
    11221 ggcagatatc tcgaaactcc gttctatgac cggggccggg atgatggatt gtaagaacgc
    11281 gcttaccgaa gcgaacaacg atatggaaaa agcgatagaa atcattcgca aaagaggtca
    11341 ggcaattgct gcaaaacgca gtgacaggga agcttccgaa ggatgcgtat tggcggactg
    11401 caaagatgga tttgccgcca tcgtagccct gaaatgtgaa accgattttg tggccaaaaa
    11461 ccaggatttt attgctctta ccaaatctat cctcgattta gctctggcaa caaagcccga
    11521 aagcctcgaa gctctgcaaa acagcactat ggacggcaga acagtggccg atctggttac
    11581 cgaacgcagc ggtattacgg gcgaaaaaat ggaattgggt tattacgaat gtgtgaaagc
    11641 tgcttctgtg atgcattacg tgcatcccgg aaataagctg gcaaccattg tgggtttcaa
    11701 caaagaatcc gaatatcagg cagcccgcga cgtggccatg caaatagccg ccatgaatcc
    11761 ggtttcgctc gacagagctt ccgtgcccgc caaaatcgtg cagcaggaac ttgaaatcgg
    11821 tcgtgaaaag gcccgccagg aaggcaaacc cgaaaatata ctcgataaga tcgctcaggg
    11881 tcgtctgaat aaattttatc aggaatccac tttaatggaa caagctttta ttaaagacgg
    11941 taagatttcg gtagctcagt acctcaagag cgtagatgaa gatttagttc ccgttgattt
    12001 caagcgtgtt accttgaatc aggagtaatc gaatccggtt gtattgaatc gggaaatagt
    12061 atttcgcttg cctgcctttc ctgctccgcc tttagttttg gattgaatca gggagcagta
    12121 tttccaatcc tataaaagaa tggtttccgc ttgactaccg ggaaccattc tttttataat
    12181 cgcctttgag cgaataccct aatttctgta agtaaggcgc gattttgtcg cgcagatcgc
    12241 cttgtagcaa aatttcgtcg tctttggccg aaccacctat gcccaaggtg cttttcaatt
    12301 gcctggccaa ttccatgagt tcctgttggc tgcccttgaa ttcacttata atggtggcgg
    12361 gtttgccgtt gcgtttttcg aaacggatgc agagctgtcc tttttttacc ggcgcaggac
    12421 tagaggccgg gttttggtgt tcctgtttcg ggttagaagc agaactaaac ctcggtgttt
    12481 cagtgttttc tgattgttct tttgagattt tggccagggc tgcaaggcgc tctttccaat
    12541 cttgtgcggg catagagttt gatcgttaat aggttattat ttatatggtc ctgccggact
    12601 ttggacaaaa ataaactgcc tttatggact cacaaaattt tgtgagtcca ttagaattat
    12661 tttattattt ttgcgccaaa tctacattta gtattgagga tgaacgaaat aaaaaaggga
    12721 tcaactgtcc ctgaacctac actcaggcgt ctgccctggt acctggcctt tcttaaaatg
    12781 gcggcggccc ggggtgaaat ccgaatatcg tctactcaga tatcgagagc catcaatgtg
    12841 agtgctcccc agattgccaa agatctttct tttctcgaaa taagcggcaa gacccgaata
    12901 ggttacgatg tacagtcgtt gatttccgtg ctggaggaat acctgggttt tacaaaaata
    12961 cgtaaagctt ttttgtttgg tgtgggtaac ctgggggccg cactgctaca cgacaagggt
    13021 ttgtcgcagt tcgggctgga aattctggcc ggcttcgatg tcaatccccg ggtggttggt
    13081 actaccattc aaggtgttcc ggtttatcat atcaaagatt accccggcat tcaagctaag
    13141 atcgatgctt ctatcggcat tttgaccgtc cccgtgcggg aagctcagaa agtagccgaa
    13201 gaaataattc aaggcggtat tcgggccatc tggaatttta ctcctttcag gattcacgtc
    13261 cccgaagata ttgttgtaca aaatacttcc atttatgccc atttggcggt gatgtacaac
    13321 cggatggaag ccaagctctg agaagatatg aagattgttg ctgtcgggat gaattatctc
    13381 cctcatattc aagaattaaa gtcggaaata cccgaagagc cggttctttt tatcaaaccc
    13441 gattcggctc tctgttccga taaacagcct tttttcctgc ccgatttctc gcaggaaatt
    13501 cattacgaag ttgaactggt ggtaaaaata agcaggttag gcaaaaacat agccgaaaaa
    13561 tttgcccatc gctattacga tgaaatcacc ctgggtctgg atttgacagc ccgcgattta
    13621 caaagaaacc tcagagcctg tggtttgccc tgggaaataa gcaaaggatt cgaccagtcg
    13681 gctgtagtag ggcattttat ccccaagaac cggtttgaaa atatacagca attacatttc
    13741 aggctcgacc tgaacggaaa aacggtgcaa cagggctcta cagccgaaat gttgtttggg
    13801 gtggaccgga taatagccta cgccagccgt ttttttaccc tgaaaaccgg cgatctgttt
    13861 tttacgggta ctccttgtgg agtggggccg gtacacaaag aagatcacct cgaggcctgg
    13921 ctcgaaggtg aaaagatgtt gcatttaagg gtaaaatgac aatagagtgg ccgaatttcc
    13981 gtttcctctc tgttggatag catcgaaact acatgaaact atataaggcg atttggagct
    14041 taatcctgag ttgtattttt acggcttctt atgcacacag cctcgattgg tattacagcc
    14101 tttctgccca gggcggaagg ttgtggtctt tcgagggagc tgtgcagatc gaagagggag
    14161 gcttgccgct ttattccgaa acttttcggc ccgcagccga tatactaagg ccggaactca
    14221 gatttccggt atgggaagaa atcaaaggca aagatttaca ggcttataag tcgctttttg
    14281 ataagtttcc cgacagtctc gaacttcaca tcaacaaagg cattcagcgt aaagaggctg
    14341 tctgggattt atacttttat cccttcctgc acaagaacgg acgttattac aggttgcttt
    14401 cctttgaatg ggcctatatc agggaggcaa ctgccggttt gctgaatgag gatgggcaag
    14461 aggatactcc gcatttcgtt tcttcgttgc aggagcaacc gggcgcagaa tcgccgggac
    14521 agcgttatgc cgaaagctcc aggttgagca gcggaaaatt catcaaaatc agtgtcgaaa
    14581 atacaggcat ctacaagctc agcttcgatg acttacgcaa aatgggcatt gatcccgcca
    14641 aagtgcagct ttacggttat gggggacagt tgttgccgga agattttact caaccctata
    14701 tcgacgattt gcccgaactt tcgttttaca tagacaagga acaaggctac ttgttgtttt
    14761 acggcctggg gcctttgtcg tggaattacg aggcttcaaa agggctgttt acccgtttga
    14821 acaaccatta ttccaccaaa ggttattatt ttgtaggtga acgttccgaa ggcaccaagc
    14881 ccatagatga gctgcccgtg ccggacaggg aacctgactc cgaagtcagg gcgtacaccg
    14941 cttataaact gcacgaaaga gatctttaca gtctcggcgg caccggcagg gaatgttacg
    15001 gcgaagattt gatgagtaat cccgacctga acctgagctt ccattttgac gacatcttga
    15061 gcggagaaca ggcaagtatc gtagttgaaa cggccgctcg cagtacctcg ccgggatcta
    15121 ctcgtcttta cgtcgacaac agctttatgg gatcgatgaa ttattcggcc gtttcttcct
    15181 ccgattctta tacttatgct cggggtagca gtttgaacaa gacttttctg cctcagggca
    15241 ataccgtcgc agtgagactg caatacaaca acaatggggt gatacctaaa aacgctcatc
    15301 tcaattacgt ggcgatcaat gcgcgttgcc gcttggtgtt gaactcgccc tctctggctt
    15361 tcagagatcc caagagcctg ggtgccggca aagtatctcg tttctgcctt gagtctgccg
    15421 atgcctttac ccgtgttttc gaaataagta aaacagggag tatgaagcaa gtggccggag
    15481 aactcaaggg aacggaatat cagttcaagg ttacgaccga cagtttgcgg gaatttgtgg
    15541 ccgtgaacct caaaggtann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
    15661 caaggttacg accgacagtt tgcgggaatt tgtggccgtg aacctcaaag gtaattttcc
    15721 caaaccggtt gtagaggggc aggttcctaa tcagaatttg cacggtttgc cggctccgga
    15781 ttttgtaatc attactcatc ccgattttat tgccgaagcc cgggagttgg ctcaggcaca
    15841 ccgcgaccac gaccggatga gtgtacaagt ggttaccaca gaacaggttt acaatgagtt
    15901 ttcatcggga actcccgacg ccacggctta tcgccgcttt ttgaaaatgt tttacgaccg
    15961 ggcaacaact gcggaggata ttcctcaatc tcttttgttg tttggagacg gggtgtacga
    16021 taatcgcctg gtaacggccg ccttttcttc ggggttttcc aatcccaaca agattttgac
    16081 cttccagtcc aaagagagtc tcgatggaac atcttcttat gtgtctgacg attatttcgg
    16141 ctttctggac gacaacgaag gagtgaatct tccgtacgat aaaatggata tcggtatagg
    16201 gcgtttccct gtacgcagca aggttgaggc caggattgtc gtggacaaaa ccttatctta
    16261 catcaacaac agagataaag gtagctggaa aaatcaactt tgctttatcg gcgacgacgg
    16321 agacaacaat acccatgtgt ctcacgccga taaactggcc gaatctgtca agagcaataa
    16381 tccgaatttt ataacccaca agatttatgt ggatgcttat cagcgggaaa ctagttctgc
    16441 cggtaccacg gtgcccgacg ccaacaaacg actgaacgaa ttgctgggtt ccggattgct
    16501 gctgctcaac tacagcggtc atggttctac caccacctgg gccagtgaac aattgtttac
    16561 cataggcgag atcaaaggaa tgcaaaacaa gcgtttgcct ctttgggtga cggctacttg
    16621 tgatttttgc cgttacgacg atgccgttac ctcgggcggg gaatatgtgt ttctcaacga
    16681 acagggagga ggtattgctt tgtttaccac ttcaagggtg gtgtattccg gaccgaatta
    16741 taccatcaac aaaagtttta tcgaccatgt atttgccacc aaaaatggcc ggcgcctgag
    16801 cctgggcgaa gtaatgtgcc tgaccaagca gtcggagtcg ctcaaacacg atagaaataa
    16861 gttaaatttt gccctgatag gcgatcccgc ccttaagttg agttatcctg aatacaagtt
    16921 ggaaataacc catgtcaatg gcagggaatt gcaggcagga gtgtacgata cgctcagcgc
    16981 actgagtaca gccgaattcg gcgggcgtgt gctgcgttac gacggaagtt ttgccgacga
    17041 tttcgacggg cttgtttacc ctgtcgttta cgatgccgaa gacttggtcg agaccttggg
    17101 caacagcggc aacgatgttt ttaagtatta cgaccgcagt aaggtgttgt ttgccggcaa
    17161 agagcaaatc agtaagggta gatttacttt ccgctttata gtgcccaaag acttgagtta
    17221 ttcctacgaa acgggtatga tcaatttgta tgcttacaat acccggggag acatcgaagc
    17281 tcagggcgtt tttaaacaat ttctgcttgg cggcaccaac gacgcagtct tgcccgacag
    17341 cacaggacct gtgatcagat tatttctgaa cgacaccagc tttgtggacg gcggaaaggt
    17401 aaacaccaaa cccagcctta ttgctttgtt gcaggatgaa agcggcatca atatgagcgg
    17461 catgaatttc ggccacgacc tggtgctgac catcgacgac aaggatcgtc atatcctgaa
    17521 caattattac gaatccgatt tgggctccgg taactcgggg cgtatcatat acaaactgcc
    17581 tgagttggaa acaggtcggc acagtcttag cctgagagcc tgggatttgc aaaacaattc
    17641 ttcggtggcc tccatcgact ttttggtggt cgacgatctg agcccacaat tgcgggactt
    17701 gaatttttat cagaacagcg aaaaagcctg gtttgtattc cgtcacgatc gcccggcgtc
    17761 ctggatgcag gtagagttca gggtgtacga tgtctggggc aggctgcaat ggcgaaagca
    17821 agtcaatatg atgaatcccg acaacagcag ccaggaactg gaatggaatt atgtgggcga
    17881 aaatggccga agagtcgaaa atggggtgta tatttgcagg ttattcgtcg ttagcgacga
    17941 cagtgaacag gcggtaaccg ccaaaaaaat catggtaggc ctgcaataaa aaggcgcttg
    18001 cagagttaaa gagtaataac ttacaaagtt tacaagatgg taaaatatgt cagcccggtt
    18061 ggggcagttg taacggcagc attgttgttt ttttcaatcg atcttagtgc acgagattac
    18121 aatcccatcg tcacttcggt accctcattg agcattgctc ccgacgcaca tgccacgggc
    18181 ctgggtgatg taggcgccgc caccgaaccc aacgtgaatt ctcaatactg gaatccctcc
    18241 aagtatgcgc aaatggaaaa ctccgcgggc tttgctcttt cgtttactcc ctggctcagt
    18301 caattggtga gcgatattaa cctgaccaac ctgacgggtt atatgaaaat tggcgatctg
    18361 cagtcgctga gtgcctcgtt gcgctatttt tcgctgggtg aggttatttt aaggcaatcc
    18421 ctgggcgatc agggttatgc catacagcct tacgagatgg ctctggatgt ggcttattcc
    18481 cgtatgcttt cgcagtatta ttccatgtcg gttgctttgc gctatattta ttccgatttg
    18541 atgagtagtg acgatccggc cgggaacgcc ttcgccgcag atatcagcgg tttctatcgc
    18601 aggcccgtat atttcggacg tgagcagggt ttctgggctt tcggtttcga cgtatccaac
    18661 atcggtacca aaatatctta cgacgccaac aacacttctt atttcattcc aaccaatctt
    18721 cgtttgggat tcagcgtgaa atatcctttc aacagctaca atgccattac ggtgtccggc
    18781 gatgtcaaca agctgttggt tcctacgcct caggactcta ccgatttcgc caccgatatt
    18841 tcgtcgatgg tgggtatttt taagtctttt accgacgctc ccggaggtct ttcagaggaa
    18901 ttgagcgaaa tcaactgggg aatcgggttg gaatacgctt acgacgggca attctttgta
    18961 agaatgggtt atcatttcga agataaaaac aagggtaacc gcaagtactt cacctttggt
    19021 gcgggattta agatgagcat gttcagtatc gacgcttcct acttgctctc tcaggctcaa
    19081 accagcccgc tggaccagac tttgcgtttt acgctgggct ttgatataga gggtataaga
    19141 aatctattag gtcgctagta tgcagttgcg tgtcggacaa ggttacgacg tacatcgtct
    19201 ggtggccggg cgcgagttat ggctgggcgg aattctcatt cctttcgaaa aaggccttat
    19261 ggggcattcc gatgccgatg tgcttataca tgccgtttgc gatgctttgt tgggtgccgc
    19321 cggtctgggg gatatcggtt tccatttccc cgacaatgcc gccgaataca aaaacatcga
    19381 cagcaagatt ttgtttaaaa ggactataga tttgttggcc gagaagcaat gggaagtgct
    19441 caatatcgat gctactcttt tggccgagag gcctaaaatt aactcttaca ttccacagat
    19501 gcaggcttgt ttggccggac tggctcaggt ggacaaagag aaaatttcca ttaaggccac
    19561 tacttccgaa aaaatgggtt ttgtggggcg cgaagagggc atggctgcta tggcggtagc
    19621 tcttatcggc cgaaagtaaa cacccagccc gcctgcagac aatgggcaat gtaatcgttc
    19681 agacctgcct gatattgtac aaaagctttg tttttgccgg tttcgaagtc cagacggctt
    19741 cttagcacaa gtgggcgatc gcccttgcct gtccagccgt agtaaccgcc cattccgttt
    19801 tcccagccca aagctccaaa atccagtttt aatttggcta catatatcgg agcgtcgttt
    19861 tggtagttgt tgttcatctg gtaacacaga aagcctccca tggcaaccaa gcggatgttt
    19921 ttgagcaagc cggcctgcga atggtaaagg tctttggcca ggctcagatc gaagtaatat
    19981 ccgggggtgt caaaaaagcg agcgcctctg cttctgtcgc tcgaagccgt tttcagtacg
    20041 atttcgccta aaatttcggg tacgtagcga ttttcggcca gaatgcgaaa ttgtgtagcc
    20101 aggtaaaaat ctccgataaa aatcagttct tcggcctctt caatgagcga ggcccgctgg
    20161 tcgcgaattt ccctgctggt gcgataatgt tcgcccaaca ctccccaggc gctcaacgaa
    20221 aggcgacccg gaacaaagac gtaactcagg cgggtggcaa ggctttgggt ttgttctccc
    20281 ggtccccaaa acaagttgcc cgaaaattcc agttggtgac ggtcgccaat aattccgtcg
    20341 taaagctccg gtaccggcag ggcgttgggt ccgaaaaagg ccggagtata ggtcatatag
    20401 tctgcccaat ccagcttttc gtcgtattga tggagttgtt tccaccagtc ggggatgggc
    20461 tgagcccgaa ttgatggagc aattaagagc agcgacagaa caaagcagca agagcaaaat
    20521 ttctccggca aagctttgaa caaccgttta cagagaggtc gaatacaatc ggcgggtaaa
    20581 cctgaagggt gggacctatc tccagtcgat ggtgacattg cggaattcca ccgaaccaaa
    20641 tttaacaaaa ggcagcttga tcaccgataa tttatcgact tttgcaggca ataccttgtt
    20701 gcgaaaagca aagacttgtc tgacggtaac atcggtaagg gcttcccagt ttttgccgta
    20761 ggcataattt tcgtctccct ggttgtcgta ggccagcagt gagttgagat aaatctgctg
    20821 atttacctgt aagttggtta ctttgatggt taattcaaaa gtttgtgctg cccggttgcc
    20881 tatgcaggaa acaaattcca ctttaatgtc tttagaggga ttgtttacgg taactgcggg
    20941 tttttcttcc tctttgggcg cttctttgga agacgtttgt acttttttgc gttgtacggt
    21001 ggtttcgaaa ggttgattgc tctcttcctg caccttgagg ctgcctgaat tctcgccgac
    21061 tctaaaggga tcgttgtcct gagcaaagct tgtacaggca atcaatagcg cagtgaggct
    21121 gaagagtatt tttttcatag ggctagttct ttttaaaata gatttttttg gaaacaaagt
    21181 cgttgccgaa tttaaagcgt atatcgatgt agttgctgtc gtcttcgtag caattgaaat
    21241 cgttgaattt aaccaccagc agatcgccct gataaacggg gttgaactgg tcggcacaat
    21301 tagacgaaat tctggcttgt tgcgaggtgg taatgttgcg ccggttgaga accagccgca
    21361 cactgacttc gccgggcttg ttgctcacct cttcgacgat gatgtttttg taagctctgc
    21421 ggtagttgtt gctcagttgt tccacctggg cgtaacgggg atcgtctttg gtgaattctt
    21481 gcaggaaagc tctgagttca ttcagggtct tgtcgataaa ttcaacgctg ggaatggtat
    21541 tcagatcgtc ggcgtattgt ttgatacggg catccaattc ggcttcgtgc gccagaaaag
    21601 cctccaccat gcgtttaagt tccaaatcgg agaagggata ttttacatgg tattggtaaa
    21661 tgctgctttt acttttcttg tcgtagatct tttcccagta ataatcgctg gccttagaaa
    21721 gcgatatttc gttgataaag ggaatgttgg cagctttggt gttgatatag gattcgtatt
    21781 cttcgctgcg ttgcctttgt gcatcgttta tactggtttc cctggtccgg atggtggatt
    21841 ccgatagcac tctggaggcc accgatcccg ccacctgagc tttgagctgg cccaggatca
    21901 tggttttggc gctctcaatg tcggaggctt cggccgaaac caccagatag cccatctcgg
    21961 caccattcac ccaggcgggc ttggatttgt ggcttttttc ttccactttc tgtgccgtgc
    22021 ccagcagagg acagagcagc aagattgtta agatgatatt tttcataaaa ctattttttt
    22081 attcggtcag ccagtccagg ttgtgcttgc tttgttccat tttttccttt gcttcgagcc
    22141 gtatgctttg atcgatttgt tcgagcattc ggagtgattt ctcgaagcag gacacgttgt
    22201 tgttgagcag aggcaccgat tccagttcga ctatggcatc gtaatacata ttacgtgcaa
    22261 taagcgattc ggcttttttg agtaaaacat cgcattcggt actgtagtag gcaacaattt
    22321 tgtccttgcc tctgactata agatttttca atctgggatt cctggcctgt aaggagctga
    22381 tagctttcaa tactgccttg ttttcgtttt cggcaatgcc tttgagagta acggattctt
    22441 gctgcaaaat ggtcttttga gtaacatcta cgataaacaa gaacacttcc atctctacta
    22501 caaattgttg gggaatggta gtggtgacgt ctttggaaag cagcgttacc ctgggtacca
    22561 gcaaaaactt acccgaggcg gctcccaggc cgttcagggc gactgcctga ttgagcctgt
    22621 tgaacaagat gtttgaggca tcggttttca gatcgatgtt ctgaggtatt tccactttga
    22681 tggcaatacg gctcgcttcg ttaatttcct gcgcaaagct tagcataaaa gacaagctca
    22741 ggccaagcaa aagggtgcat atctttttca tattattttt ctcccaatat caaccaaact
    22801 tctcctaaac ccctttcgta tatcttactt tggatattga aaggttcggc tttgagcata
    22861 gtgctcaggc cgttgatgaa accgcgtgca tcgatggcac gttccctgcc gaaagacatt
    22921 ttaaacaaag ggatacgcac ttgttcgtaa cgtatggtgt tttcggtggc cgacactctc
    22981 gaaaagcggc tgtttaccgt gttgtcgtcc atccagacgt ccatgatatc gcccagcatg
    23041 ccggtttcgc ccatgtattc aaaatcggtt tcaaagtcca catcggcgtt gtcccatacc
    23101 cggagcacca ccaccacttc gcggccgttg gtaaaaatgt cgtcaaagtg attctgcaga
    23161 gccgaggtat agtcgtccat gtaattgagt accgcttctt cgagcaagag ccctacggag
    23221 gcagaactcg aaggtttacc gtcgccggca accgccgtaa tcaccttgtt ggtataagcg
    23281 tcgaccgctc gcaggttgta ggtgatgaag cgttgaggac ctcttatctt gaccgaaaag
    23341 tcgatatcca taatgatatc tgcctgagct gtgcgcttca gtacatcgat gggcgattct
    23401 ctgatggcgc tgcccgagct gctgctttgc agcaaggata attccacatt ggactgagct
    23461 atcgatttca tggtctgttc cagatctttc agagggaagc ccctttctgc catgattttg
    23521 gagagttccg aaatcaccag tctgagttct tcactttcag cgcttttgaa ggccttgttg
    23581 taatcggaaa tgcttctggt attgcctgcc tcgtcggtaa attcctggac aaaggcattg
    23641 cggtcgcaat agctgtcgct ggggacaacc atcagtatgg gttttttggc ctgtcccgaa
    23701 acatttagta tcgtggcggc cagtgctata catagtaata ggttacgcat attgtatgga
    23761 ttattaagtt aaaaaccgtg gcctaatttt ttgactatgc ctttttcttc catatagctc
    23821 cgcaggttgt tgtacataac ctgcacgtct atggcaattt tgtagcctcc tttcatctga
    23881 acgcggtatt ttcccgaagg tggttcgtca gtggtgcggt ttacaaaacg caggtaggct
    23941 cctccattgc cctttttgtc gggataggca aagaattcct gaaaaaaggc ttcgtgggtt
    24001 tcttcgtcac tgagcttaca tatggccggg gtctggttgg tatgtccgct gccgggcaaa
    24061 cccgaaaaga ggcaggcagc cacggcatct cgcttggctt tgttgatggc cgcttccacc
    24121 gtcttgtcgt aggcccagat cctgaacacc ttggtgccat cctggccaac ccccagcacc
    24181 tgaacttcgt aactggcagc acttttaaac attcctttgt tgacagaccg ggcactttgg
    24241 gcgtctaacg ggagtgtggt tagacagaac aaagtggtca acattaaagt aaatagatac
    24301 ttggttttca taatcttttt ggtcaaatga tatatatata attaaaaaca atagcggata
    24361 cccagagccg tctgagaccc gttcatatcg cggaaattac ccatgttgtt ggatacgcct
    24421 tcataggcct ggtagatatt gcctttacca aaaaccgcat tgtagcttac attccagaac
    24481 aggtacaccg gataataaat attgacttgt gcccgtaaac caccttgcag cacgtaggct
    24541 cttaaatcgg ctttttcgta agcgtcgctg ccatcgaaac tgtaggtatc cataccgaaa
    24601 ccggcaaaag gataaatttc aaaattgggt atacgcagac cgtaaccata actcaaaatc
    24661 aagttcattg cactgccgtc cagattgaaa gccaaagaag tataggaaca aaacctcctg
    24721 ccggtatggt tctcgtagga aaggtcggca tgaaatccgc tattgacggg ccctccttgt
    24781 ggaatactgt tgaatcccac tccgagtgaa agattcaggt cttttttctc taccagggta
    24841 agcccctcga ccaccctgcc gtaaccggct attttggtaa attccgattc cgggctctcg
    24901 ccggtggcta ccgtacggtt gtctgccacc tttttggtgg ctcgcaaaat gcctttacgc
    24961 accagtttga ttccgccgtc gctgttgccc ttgaagccgt aggcataaaa tcgcaggccg
    25021 tgtctgacgt cttctttggt gccgattttg gctctgatgg gtcgcaggct ggtaatggga
    25081 gtggtaacaa taaagtcttc gtgctgtttc tctattttat aattcaggtc gctgtaaatt
    25141 tgctcttgca gttcgttgaa agcgttcgca aggcgttgtt cgtctgttat tttcttgttg
    25201 ctcagcaccc cccgtctttt gttggcctct atgcctgtgg aaacgctttt atcgccgaga
    25261 tttacccgtt cgaccaattc cacaggtatg ttccaggctt caaatgcggc ttttttggct
    25321 tgcctttctt cggcgggtgt gttttcgtcc agccagtact tatcgtaaaa agtattcagg
    25381 tcttgttcgt cccagttgag cttgtataaa tacagggcgg cggtggcctt ccaatagagt
    25441 tcgcggttgt cgtccttacc ggaatattcc gttcgcaggt tggttacatc gtagaccatt
    25501 aaataactcc ggcctatcag cccgctgccg gcatcgcgga cggtttctac cttgagggta
    25561 ccgctcaggc gcatatctac gtcgtgggcg ctgtattccc cacgttcaaa tacccggtcc
    25621 agattcatgt tgccgtccgg ctgccggttg aaaatataag ctacaatttg ttggcctgcc
    25681 cgttgttcat tcagcatgga gctgataagt tcggtacggt cgggcgcttt gggccaaatg
    25741 ctaccctcgg ggaagaagtg ggcacgaga
//
LOCUS       DAOSTD010000023        24179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_2260_length_24179_cov_14.233958, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000023 DAOSTD010000000
VERSION     DAOSTD010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24179
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2260_length_24179_cov_14.233958"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2437)
                     /gene="infB"
                     /locus_tag="PLI37_02780"
     CDS             complement(<1..2437)
                     /gene="infB"
                     /locus_tag="PLI37_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOQ57978.1"
                     /translation="MSSVRLNKITRELNVGIGTLVEFLQKKGFEVENNPNAKISGEAY
                     MLLVEEFGDGQELLIPKKEKPKIRETEQVTPEPVKQEAPEEPVVSTRKQQFKTLGKID
                     LDAVAKPKATGPKKEEKEEILPKEEKPAKTVEEVITAVASPASTEPEAAEKIKKEESP
                     VQESAKKAPAAEAPVSRTPAADMAEKEEKPTPQKETVRTEEKTIVEEKEEIPETIPPQ
                     KIEEQEDAQEDKKSEEPEIFTLGKRTIKPNIKVVTTIDLDALNQNTRPKRKTKQERLK
                     EQEDKKTQEQALREERKKAETLKGRTEGKTEVSDSKKKRRSRIKDVKIKVDGEFGAPV
                     SRGDRTKGRLKKPVLKTEVSEEDVSKQIKETLARLTSRPKKGAKYRREKREAASQRMQ
                     ELEQKEMREKSVLKITEFVTVNDLATMMDVPVVDVISTCIAIGQMVSINQRLDAETIN
                     FVADEFGFKTEYVSAEVLEAIQEEEDDEEDLVPRPPIITVMGHVDHGKTSLLDYIRNS
                     NVIAGEAGGITQHIGAYNVTLKDGRKMTFLDTPGHEAFTAMRARGAKITDIAIIIVAA
                     DDNVMPQTVEAINHASAAGVPMVFAINKIDKPAADPEKIKQELANMNYLVEEWGGKYQ
                     SQDISAKKGINVKELLEKVLLEAEMLELKANPSRRAVGSIIESTLEKGRGYVATVLVE
                     NGTLEVGDNIVAGVNHGRIKAMFNERGQRVESAGPAEPVLILGLNGAPQAGDNFNVLD
                     SEQEAREIATKREQLQREQGLRTSKILTLAEIGRRIAVGNFKELNLIVKGDVDGSIEA
                     LSDSLIGLSTEQIQ"
     gene            complement(2452..3723)
                     /gene="nusA"
                     /locus_tag="PLI37_02785"
     CDS             complement(2452..3723)
                     /gene="nusA"
                     /locus_tag="PLI37_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOQ57979.1"
                     /translation="MAKTEERINLVEIFSEFKESKNIDRSTMINVIVESFRGVIAKLF
                     GSDEHYDVIINPDSGDFEIWHNREIVEDDQVEDPEHQISLTEARKIESDYEVGEEVTD
                     QVHFSAFGRRAILNLRQTLASKIMELQKESLYNRFIEQVGQLISAEVYQIWKKEILLL
                     DDENNELILPKSEQIPSDFYRKGETVRAVIERVENKTNNPKAVDPKIILSRTSPVFLE
                     RLFELEVPEIHDGLITIKRVARIPGERAKIAVESYDERIDPVGACVGVKGSRIHGIVR
                     ELRNENIDVINYTSNTSLFIQRALSPAKISSIRLDEVNKKAEVWLKPEEVSLAIGKGG
                     MNIKLASMLTNYTIDVFRETDQELEEDDIYLDEFKDEIDEWVIEALKAIGCDTARNVL
                     ALPRETLVEKADLEESTVDEVLRILHAEFED"
     gene            complement(3770..4237)
                     /gene="rimP"
                     /locus_tag="PLI37_02790"
     CDS             complement(3770..4237)
                     /gene="rimP"
                     /locus_tag="PLI37_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOQ57980.1"
                     /translation="MISKEIINQYIEEWINNKPFFLVDLNIGPDFSIAIEIESVQGNV
                     SIGDCAELSRHIESRLSNEDLDFALKVSSAGIGQPFKVFRQYQKHINKEVEVRLKNGQ
                     KFSGTLLSCDEDKFTVNVMRKIKPEGAKRPKIIEQQECYLQAETASVCSKIIF"
     gene            4403..5059
                     /locus_tag="PLI37_02795"
     CDS             4403..5059
                     /locus_tag="PLI37_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOQ57981.1"
                     /translation="MKSVFYPLLIFWSCFTLFCSCSTISNIVKKEFAERNPEDILQNT
                     AAEYIVNSGIEGEAGQWIASQMEILKIMLSEQIGDSVMIRQPEEGLLLVFDSDRLFKQ
                     GLQYVNDSSIETLEVLATSLQDFPNTRLYILVHTDNSSSEFIALSLSDKRAKNIKRIL
                     VSKGVKRSHITAEGRGFMVPVISNESPEGRKINRRVEMAVTANHKMIRQAIKSTKTKR
                     "
     gene            5107..6633
                     /gene="gpmI"
                     /locus_tag="PLI37_02800"
     CDS             5107..6633
                     /gene="gpmI"
                     /locus_tag="PLI37_02800"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOQ57982.1"
                     /translation="MSKKAMLMILDGWGIGNRGKEDVIYNTPTPYWDYLIKNYPNSQL
                     QASGENVGLPDGQMGNSEVGHLNIGAGRVVYQDLVKINISCRDNSILENPGIKAAYGY
                     AKQSGKQIHFLGLVSDGGVHSSLDHLFKLIEISKLYGIKKSFVHCFMDGRDTDPKSGK
                     SFIAALQKHLDRNTGAIASIVGRYYAMDRDKRWERIKEAYDLLVKGKGVKAQDMVQAV
                     QASYDQGVTDEFIKPIVHTDAQGQPLATIQDGDVVLFFNFRNDRARELTIVLTQEDMP
                     QHGMQTLCLEYYTMTPYDATFKGINVLFDKENVDNTLGEIVAKAGKKQLRIAETEKYA
                     HVTFFFSGGREKEFENEDRILIPSPKVATYDLKPEMSVNEVGDALKQAIETEKYDLIV
                     VNYANGDMVGHTGVYSAIQAAVVAVDANIKKVVEAAKSKDYEVIIIADHGNADNALNP
                     DGSPNTAHSLNPVPFVYISRNSSAEVSDGILADVAPSLCHILGIKQPAEMTGRCLIHD
                     "
     gene            6643..7230
                     /locus_tag="PLI37_02805"
     CDS             6643..7230
                     /locus_tag="PLI37_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOQ57983.1"
                     /translation="MLQIDKTIVSLDIINQTFCCDLNKCKGRCCVEGVSGAPLEEDEI
                     AELEKALPAVWDELSPRARQLINEQGVCYKDASGETVTSIVDGKDCVFTYYDEKGVCY
                     CALEKAWREGKSSFIKPVSCHLYPIRLSKLTSGVVALNYQQWHVCVPALAKGKTLGLP
                     IYRFLREPLIRKFGQDWYDQLCEAAKMLEDYPRDV"
     gene            complement(7336..7689)
                     /locus_tag="PLI37_02810"
                     /pseudo
     CDS             complement(7336..7689)
                     /locus_tag="PLI37_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
     gene            7790..8716
                     /locus_tag="PLI37_02815"
     CDS             7790..8716
                     /locus_tag="PLI37_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ57984.1"
                     /translation="MKKQPIILCFFLWTALAAFAQERIDLPLWPDGVPESNGITAEEI
                     NENNYFSNVSVPAMSVFKANPDKNTGAALIICPGGGYARQAAGHEGFQLTGWLNENGI
                     TAIILKYRLPNGHDFIPSKDALQAIRIVRSKAEEWGINPSKIGISGFSAGGHLASTAG
                     THFDYGNPEAEDPIARQSSRPDYMILFYPVISMKEGVTHVGSKRNLLGDNPTKEQMLE
                     YSNELKVSAETPPTLLLLSDDDKGVIPQNSLDFYAALKNHKVPAAMYIFPEGGHGWGY
                     KESFRYHHVWRILMIEWMMQQKILPDMIRQQN"
     gene            complement(8722..11196)
                     /locus_tag="PLI37_02820"
     CDS             complement(8722..11196)
                     /locus_tag="PLI37_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOQ57985.1"
                     /translation="MKNQSSKGTKRLKILTGLFLPLVVLFLLVPLPKFKYPCSTVVYA
                     ANLELLGARIAADGQWRFSPEGEIPDNYVKALILYEDNYFRYHPGVNPVSLGRALVQN
                     LKAGRIVSGGSTLSMQVARMSRNNPPRTLSGKCLEILMALKLELCYSKDKILALYAGV
                     APFGGNVVGITAASWRYFNHPHQKLSWAEAATLAVLPNAPALIHPGRNAAELKQKRDA
                     LLGKLYQKKHIDSLSYQLALAESLPQAPLPLPGLALHVVEKAHKQFPGQNVYTSIDYP
                     LQEKCIALLEQRSKELEQSEIHNLAALVCDVKQNSILAYAGNISAVAQLDVFVDIVQT
                     PRSSGSILKPFLYASMLHQGEILPNTLIADVPVNFSGFSPKNFDLNYQGAVPASEALS
                     QSLNVPAVEMLHQFGEARFLDVLKNLGFTTFVYPSQHYGLSLILGGGECNLLELAGAY
                     ASLARVLNRFGSVQEYSSEDYRPLRLFRENSPEKKSDTKAFHKTNISRFWPEIFPNKR
                     KNAVSKGRKSASENEESLPLLSAASIWCTMEALRQVNRPRERSGWKHFESSRRIAWKT
                     GTSFGLRDAWALGATPEHVVAVWAGNADGEGRPGLTGLRAAAPALFDLFDLLPQSGAW
                     FEQPDSEMNLEEICMESGCKASGYCTRKSLQMVPASGSKTELCPYHRLVHVSKDGKFR
                     VNASCYPLELIRSDSIFVLPPAMEWFYRKTHPSYRGLPPLMESCGEEKEYKQMEILYP
                     RNPYSLYIPRELDGSPGKLVIEVVHRKADALLFWHLDDEWIGNTRRIHQMSISPAPGN
                     HTLSIYDQQGNNLIQHLRIQAGRVER"
     gene            complement(11224..>13473)
                     /locus_tag="PLI37_02825"
     CDS             complement(11224..>13473)
                     /locus_tag="PLI37_02825"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOQ57986.1"
                     /translation="RPEKEYTIRVSESNKQAMDYTIALVDEGLLDLTNFSTPDPWAHF
                     YAKEALGTKTYDMYNEVLGSFGSRLESMYAIGGSDQEIDYSKKKADRFVPIVEVLGPF
                     RLQAGKKAEHKIQLKPYAGSVRAMLIAAGRGRYGSMEKSIRVSEPLMLYASLPRTLAL
                     GEKADLPLTVFAMDESIKTVRIRFETEGRLQGSADTLILFDTPGQQDVFFDLKAAGEA
                     GLGIIKLSAVSGREKTQHSIELDVMHNNPPSTEQVFKMLPSGESWQANLSALGVPGTN
                     KARLEISSVLPLNLEYQLSFLNQYPHQCAEQVVSAVFPLIYVPKLLELSAEKRKEYEQ
                     NIKDCIDRLPRYQSMDRGFAFWPGRSVGELWLTSYLTYFMLEAEKAAYLIPSNLKRAG
                     LDYLANGVRRYREYLPDYKAQIQAYALFVLARAGEPEVAAMNRLMKADCLNNQGKWLL
                     ASAYAASGMKEAAYKLIDLRDTEPGTVSAYTYGSVLRDRCYLLQSLMLLGEWEQAAPL
                     ALKIATDFSRAGRHSTQTTGFVLSVLSEFCLLTSATSEAETELYVNGKPQQTRPSGAF
                     TLVDLSYGRDLELDLRLVNKGKGTVFASLFNQGVKAGMDASEQEKGLALEISYMDQEG
                     KKLNVSKLKQGTNFIVQLRISNKTAFAVDNLALTQMIPAGWEIINERLFGTGSASEDQ
                     NNYDYRDIRDDRVNTYFSLGAGKTKVFTLELNASYCGDFILAPVLCEAMYDQAYYARK
                     GGAKVKVER"
     assembly_gap    13474..13573
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13574..16865)
                     /locus_tag="PLI37_02830"
     CDS             complement(<13574..16865)
                     /locus_tag="PLI37_02830"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOQ57987.1"
                     /translation="MKQINKIAFILLVAIGTVACGNRKDKKFEKDLSDYSGIISAISS
                     GAIRRADPIVVVFTKDIVEKELIGKTLEKNPFVFKPEIKGKCSWKSPNTLVFKPEKPL
                     NWNTNYEASLNLEYLLKLPEKLEKKLNFSFFTAKKQLKLSYDELRLDEGGNYFLEVYI
                     NTSDRFDSHEIEQSLIVEYPHEQLSLKWEHNPETFSHTLSIRNIVREKENSELVLNIK
                     PIDNSYKPSISQYSFTIPGTDVFMLSSVKVKESPDQVLEIVFSDLLNMQQDLRGLVSL
                     DEQKSVKYTIDNNVLKIYPSNTLSGEFTLNISRSVENKQGKTLQKNYSQKINFGRIKP
                     AIRLLGKGVIMPQSQGLILPFSAAGLKAVDLRVIQIFPNNIVAFFQDNDIDETYKWGL
                     NRVGRTIVTKKISLEDKGAEDLTNWNAFSLDLASFIDLEPGSIYNVELGFRKAYSLYE
                     EQASEETDHYLPIDEEMLFPGQSYENTYYNYYYDWRNYENPASQAYFSPNRFVNRNVF
                     CSDLALIAKMDAKGQMSIYVSNLMTTASEAGVQLSVYDYQNQLLTRAQTDGQGYFRFT
                     PERAPFVLFAQKGNQYAYLKLDNSSAIPVANFDTDGIKAEEGIKAFFYAERDVWRPGD
                     SIYLHLILEDREQQLPEGHPINMEIYNPRGQFVKKILTLRDERCIYPFYFATSKDDPT
                     GNWKVVVKAGTHIFQRGIQVESIKPNRLKIELEIREDVLSKSETSWAKLKASWLHGTP
                     AANMKAQVSAVLHPYTPTFEKYKDFDFSTPYPRQSSKEFTLFDGQLDYKGEASVTMSF
                     GPGRDFEGYMQARISTKVYEGGGFSINQRSIPFIASEHFVGLKMNYSYPSWKKLDNDK
                     NHRVEVVSVDEKGNPADLSNIEVKLYDLDYHWWYQSNSNLLASYAGQTYHNPVYSTKI
                     STNKGKGSFELSNNSQRYGCYLLLVTAPDGHTCGQQIYFGLPWGSGIQKGDAQVLALV
                     IERDQYKTGETASLSFPAGKDARALVTLENGKGILHQEWLDNLEAMNEYRFVTTPDMA
                     PNVYVNIMLIQAYAQTANDLPIRLFGLTSFRVEDEQTRLHPVLDIPDELRPEKEYTIR
                     VSESNKQAM"
     gene            complement(16867..17475)
                     /locus_tag="PLI37_02835"
     CDS             complement(16867..17475)
                     /locus_tag="PLI37_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOQ57988.1"
                     /translation="MNYQLVLDKTLEEIKAENKKPTLLLHVCCAPCSTYVLEYLSDYF
                     TIALYYYNPNIFPKEEYDKRLWTLVKFVRELKSEIRPIIEEANYDPSEFYRNIEGLEN
                     EKEGGGRCQKCYALRLEEAARKATEKSLDYFTTTLSISPLKNAQLINDLGAVYENRYG
                     VRYLYADFKKKEGYKRSIELSKQYGLYRQDYCGCEFSMKIKR"
     gene            18140..20764
                     /gene="mutS"
                     /locus_tag="PLI37_02840"
     CDS             18140..20764
                     /gene="mutS"
                     /locus_tag="PLI37_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOQ57989.1"
                     /translation="MSNNISNTPLMKQYFSIKAKYPDAVLLFRVGDFYETYGKDAILA
                     AEILNIALTFKTAGKTGKIEMAGFPFHALDTYLPKLVKAGKRVAICDQLEDPKKTKTL
                     VKRGVTELVTPGVSTNDAVLDHGENNFLAALHLDQKRAGVAFLDISTGEFITAEGSFE
                     YVDKLLSSFNPKEILYERSKRSLFQDTFHYNGPRFEMEDWVYTDDSAQRRLLKQFQTV
                     SLKGFGIQNMPLAIIASASVLYYMDMTQHLQLGHIQQISRIDEDNYVRLDRYTIRNLE
                     LCNTINQEGKSLLEILDKTCTPMGKRQLRRWVLFPLKDIKAIEARQEVVEFFMNNKEI
                     HQYVKERLSLVGDLERIVSKVAVGRVSPREVVHLKNALMSVADIKQYLNSQSLEITPP
                     LMSMIQAVDTCEDLCLRITTELVENPPIALNRGEVIRAGVNEELDQLRNLSTSGKEYL
                     QRLQERESQKTGISSLKVSFNNVFGYYMEVRNVHKDKVPENWIRKQTLVSAERYITPE
                     LKEYEEKILGAEERISQIESDIFNALVLDLVSFVSIIQNNAVQLSQLDCLLSFAEVSS
                     LNNYHKPQLSDSDEINIKAGRHPVIEKQLPDGEIYISNDVFMDNKSEQILIITGPNMA
                     GKSALIRQTALIVIMAQTGCFVPADEAQIGLVDKVFTRVGASDNLSLGESTFMVEMLE
                     AADILNNITPRSLVLFDELGRGTSTYDGISIAWAIVEYIHEQPKARAKTLFATHYHEL
                     NEMEKTFPRIRNYHISVKEADNKVIFLRKMRRGGSEHSFGIHVAKMAGMPPSIVNRAN
                     QILQEFETSQQGKVPAKPVSRLAETREGLQLSFFQLDDPVLSQVRDEILKVDINNLTP
                     IEALNKLNEIKKIVKGRG"
     gene            complement(20770..21873)
                     /gene="ychF"
                     /locus_tag="PLI37_02845"
     CDS             complement(20770..21873)
                     /gene="ychF"
                     /locus_tag="PLI37_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOQ57990.1"
                     /translation="MALQCGIIGLPNVGKSTLFNCLSNTKAQAANFPFCTIEPNVGVI
                     TVPDERLNELAKLVNPKRVVPTTVEIVDIAGLVKGASKGEGLGNKFLGNIRQTDAIIH
                     VLRCFDDSNIAHVNGAIDPVRDKEIIDYELQIKDLETIDSRINKVQKQAQTGGDKQAK
                     VAYEVLLRYKLALEQGKSARTVKFDTKDEQKIAHDLFLLTNKPVLYVCNVDEASILKG
                     NRHVDMVREAIQEEGSELLVVAAKIESEIAEFEDAEERSLFLQELGLKESGVNRLIKA
                     AYKLLNLSVFLTAGPLEVRAWPFKTGSKAPVCAGIVHSDFEKGFIRAEVIKYEDFIRF
                     GSEQAVKEAGKMHVEGKDYVVQDGDVIYFRFNV"
     gene            complement(21913..24141)
                     /gene="polA"
                     /locus_tag="PLI37_02850"
     CDS             complement(21913..24141)
                     /gene="polA"
                     /locus_tag="PLI37_02850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOQ57991.1"
                     /translation="MKRISASNSYADSEKKPSGHSEGEKKLFTPIFVEASQPGASKKK
                     PAGLNLQNSLWDEQGNAVGSSSLPTKIKTQQNIVGNDLFSNNLSAINPDESTDSLPES
                     ILSSLKNTAHYYHILDNEKDIGGFIKEFSESDFFAFSTKKQGTEAMNAQMTGIAFSKE
                     KNKAYYLPCPNNRKKCKELLNSFKPLFENKHSLKIGYDLKNEILLLRNYELSLAGPLF
                     DILIAHYLIQPELRHDLDFLSKSYLNYQCLIKEGLPELKAKKRVESQDLNAEQSLRVE
                     QDLGVQQSLSAEQNLSAEQDLSAEQDLNVKMAAEFICEQADLYLQLKSKLETEIEQGG
                     LNNLYYEIEAPLIYVLADMENSGVYIDTEALKNSSVLLTEELNGIEQEIYGLAGTTFN
                     VNSAKQVGDVLFERLRIVEKAKRTKTGQYSTSEEVLETLRSKHPIVSKILNYRGLKKL
                     LSTYIDALPPLINPKTGKIHTSFNQAVTATGRLSSSNPNLQNIPIRDDKGREIRRAFS
                     AGPGELFLSADYSQIELRVMAHLSEDENMIEAFRLDQDIHAATAARIFKLPIEDVDQD
                     MRRKAKTANFGIIYGISTFGLAERMGVSRAEAKKLIEDYFETYPKIKAYMDHSVQMAR
                     ENNWVETVFHRKRYLPDIQSRNAVVRSYAERNAINAPIQGTAADLIKIAMVRIHLALE
                     SAQLKTKMILQVHDELDFIVPEEELEQVKKIIIYEMENAASFKVPIKAEIGVGKNWLE
                     AH"
BASE COUNT         6303 a         5602 c         5251 g         6923 t
ORIGIN      
        1 cctgaatttg ctctgtgctc aaacctatca gagaatccga caaagcttca atagatccgt
       61 ccacatctcc ttttacaatg agattcagtt ctttgaaatt gccaacggct atacggcggc
      121 cgatttcggc cagtgtcaga attttactgg tgcgcaaccc ttgttctctt tgcaattgct
      181 ctcttttggt ggcaatctct ctggcttcct gttcggagtc gagtacgttg aaattgtcgc
      241 ctgcctgagg agccccattc aatccaagga tcaatacagg ttcggcgggc cctgccgatt
      301 ccacgcgctg ccctcgttca ttgaacatag cctttatgcg tccgtgatta acgccggcaa
      361 cgatgttgtc tccgacttcc aacgtgccgt tttccaccaa tactgtcgcc acgtagccac
      421 gccccttttc gagtgtagac tctatgatgg accccacagc acgtcttgag ggattggcct
      481 tgagttccaa catttcagcc tcgagcaaaa ccttttcgag caattccttc acattgatgc
      541 cttttttggc tgaaatatct tgtgattggt attttccgcc ccattcttcg accaggtaat
      601 tcatattagc cagttcctgc ttaatttttt cggggtcggc tgccggtttg tctattttgt
      661 taatggcaaa taccatgggg acgccggctg ctgaagcgtg gttaatggcc tcaactgttt
      721 ggggcataac gttgtcgtcg gctgccacaa taataatggc aatatccgtt attttggctc
      781 cgcgggcgcg catggccgta aaagcctcgt ggcccggagt gtccagaaaa gtcattttcc
      841 gtccgtcttt cagagtaaca ttgtatgctc cgatgtgctg agttatacct ccagcctcac
      901 ctgcaatcac attagaattg cggatgtagt cgagcagaga agttttaccg tggtccacgt
      961 gtcccattac cgtgataatg ggaggacgag gcaccaaatc ttcttcgtcg tcctcttctt
     1021 cctgaatggc ctccaatact tcagcactga catactctgt tttaaagccg aattcgtcgg
     1081 ctacaaaatt aatggtttct gcgtccaggc gttgattgat cgacaccatc tgacctatgg
     1141 cgatacaagt ggaaattacg tcgacaaccg gcacatccat catggtagcc aggtcgttaa
     1201 cggtaacaaa ctcggttatt ttaagcacgc tcttttcccg catttctttt tgttcgagtt
     1261 cttgcatacg ttgagatgcc gcctctcgtt tttctctgcg gtattttgct ccttttttgg
     1321 gcctgctggt caggcgggca agtgtttctt taatttgttt ggaaacatcc tcttcgctga
     1381 cttctgtttt aagaacgggt ttcttgaggc ggcctttggt cctgtcgccc ctggagaccg
     1441 gagcgccgaa ttctccatcg acctttatct tgacatcctt gatgcgactg cgccttttct
     1501 ttttggaatc tgaaacttcg gttttcccct cggttctccc tttaagagtt tcggcttttt
     1561 tcctttcttc gcgcaaggcc tgttcttgtg tttttttgtc ttcctgttcc ttgagtcttt
     1621 cttgtttggt tttccttttg ggcctggtat tttgattcaa cgcatccaga tcaatggtgg
     1681 taaccacctt gatgttgggt ttaatagtcc ttttccccag ggtgaatatt tcgggctctt
     1741 cagatttttt atcctcttgg gcgtcttcct gttcttcgat tttttgagga ggtattgttt
     1801 caggaatttc ttctttttct tcgacaattg tcttctcttc cgtgcggacg gtttccttct
     1861 gaggggtggg tttttcctct ttttcagcca tatcggctgc aggtgttcgg ctcacaggag
     1921 cttctgctgc cggggctttt tttgcagatt cctgaacggg ggattcttct ttttttattt
     1981 tttcggctgc ttcaggctcg gttgatgccg ggcttgcgac agcagtaata acttcctcaa
     2041 cagtttttgc gggtttttct tccttaggaa ggatttcttc cttttcctct tttttcggtc
     2101 ctgttgcttt gggtttagca acagcatcca ggtctatttt acccagagtc ttgaattgtt
     2161 gtttgcgagt gcttacgaca ggttcttcgg gagcctcttg cttaacaggc tcaggagtta
     2221 cctgttccgt ttctctgatc ttgggtttct cctttttggg aattaaaagc tcctgcccat
     2281 cgccaaattc ttcgacgaga agcatatagg cctctccgct gattttggcg ttggggttat
     2341 tttcaacctc aaatcccttt ttttgtaaaa attccaccaa agtgccgata cccacattca
     2401 gctctctggt tattttattc aatcgaacac tcgacataag caaataattt attaatcttc
     2461 aaactctgca tgaagtatac gcaagacttc atccacagta ctttcctcca aatcggcttt
     2521 ttcaactaaa gtttctcttg gtaaagccaa cacattccga gcagtatcgc aacctatggc
     2581 ttttagggct tcaataaccc attcatctat ttcgtccttg aattcatcca ggtaaatatc
     2641 atcctcttcc agttcctggt cggtttctct gaaaacatct atggtataat tcgtcaacat
     2701 gctggcaagc tttatgttca tgcctccttt gccaattgcc aatgaaactt cttcgggctt
     2761 gagccatacc tcagcttttt tgttgacttc gtccagcctg atagaagaaa ttttggcagg
     2821 actcaaggct ctttgaataa acaaactggt gtttgaggta tagttgatga cgtcaatatt
     2881 ctcgttgcgc aattcacgta ctatgccatg gattctggag cctttcacac ccacacaggc
     2941 tccgaccgga tcaatacgtt catcgtaaga ctccacggcg atttttgctc tttctcccgg
     3001 aatgcgggct acccttttga tggtaatgag cccgtcgtgg atttcgggaa cttccaattc
     3061 aaaaagcctc tcaagaaaaa caggagaggt acgcgacaaa attatcttgg ggtcaacagc
     3121 tttgggattg ttagtcttgt tttcaacgcg ctctatgacg gcacgaaccg tttctccctt
     3181 tcggtaaaaa tcgctgggta tctgctcact cttgggtaga atcagttcat tattttcatc
     3241 gtccaaaagc agaatttctt ttttccaaat ctggtagact tcggcactta tcaattgtcc
     3301 cacttgttca atgaaacggt tgtagaggct ttccttttgt aattccatga tttttgaagc
     3361 cagggtttgc cgcaaattca aaatggcgcg acgtccgaag gcactgaaat gtacctggtc
     3421 ggtaacttcc tcacctactt cgtaatcgct ttctatttta cgggcttctg tcaggctgat
     3481 ttggtgttcg ggatcctcca cctggtcgtc ttctactatt tcgcgattgt gccaaatttc
     3541 aaagtccccg ctgtcggggt tgataataac atcgtaatgt tcgtcagagc caaacagttt
     3601 tgctatgact ccacgaaaag attctacaat cacatttatc attgtagaac ggtctatgtt
     3661 tttagactct ttgaattccg aaaaaatttc gaccagattg attcgttctt ctgtttttgc
     3721 cattgttatc gacattgtgt tgaaatatct attaattctc tcgtatttat cagaatatta
     3781 ttttggaaca tacggaggcc gtttccgctt gcaaatagca ctcttgctgc tcaataattt
     3841 tgggcctttt ggcaccctcg ggctttattt ttcgcataac gttgacagta aatttgtcct
     3901 cgtcacagct taataacgta cctgaaaact tttgtccgtt ttttagacga acctcaacct
     3961 ccttgtttat gtgtttttga tattgcctaa atactttaaa gggttgacct atgcctgctg
     4021 aagaaacttt cagggcaaaa tccagatcct cattgctcag acgggactct atatgcctgc
     4081 tcaactctgc gcaatcgcct atacttacat tgccttgaac agattcaatt tctatggcaa
     4141 tactaaaatc ggggccgata ttgaggtcaa ccaaaaaaaa gggcttgtta ttaatccact
     4201 cttcaatgta ctgattgata atctctttac taatcatcct tttgtgttaa gtaaaaaatg
     4261 aggggaactt ttgtccccct cgcctgtatc ctaacggccg caaaattaca caatatcaag
     4321 tgatttgcaa aaaaaacgca gattatttta atctggaggc taaaaaatat acttttgcgc
     4381 ggactttgat ccgaaatatt gtatgaaaag cgttttttat ccccttttga tattttggag
     4441 ctgttttaca ttgttctgct cttgctctac catttcgaat attgtaaaaa aagaatttgc
     4501 tgaaaggaat cccgaagaca tcctccaaaa caccgctgcc gaatacattg taaattcggg
     4561 catcgaggga gaagccggcc agtggattgc ctcacaaatg gagatcctga aaatcatgct
     4621 aagcgaacaa ataggcgatt ccgtgatgat tcgccaaccc gaagaaggcc ttttgctggt
     4681 ttttgactcc gaccggcttt ttaaacaagg ccttcagtat gtaaatgatt cttccatcga
     4741 gaccttagaa gtacttgcaa catccctgca agattttcct aacaccagat tgtatatcct
     4801 tgtccacacc gacaacagta gttcagaatt catagcgctt tccttatcgg acaagagagc
     4861 caaaaacata aaaagaattc tagtatcaaa gggcgtgaaa cgcagtcata tcacagccga
     4921 aggacgaggg tttatggtgc ccgttatttc gaacgaaagc ccggaaggcc gtaaaataaa
     4981 ccgccgggta gaaatggctg taacggccaa ccataaaatg attcgccaag ctataaaaag
     5041 cactaaaaca aaaagataat tcagccgaat tgcttacctt tgcgcccgat attttagata
     5101 actattatga gcaagaaagc aatgctgatg attctcgacg gttggggtat tggcaaccgt
     5161 gggaaagaag acgttatata caacacccct actccttact gggactatct gataaaaaac
     5221 tatccgaact cgcaacttca ggcatcgggc gaaaacgtgg gtttacccga cggtcaaatg
     5281 ggcaattccg aagtgggaca tctcaacatc ggagccggca gagtggttta ccaggatttg
     5341 gtgaaaatca atatttcctg ccgcgacaac agcattctcg aaaatcccgg catcaaggca
     5401 gcctacggtt acgcaaaaca atcgggcaag caaatccact ttctgggcct ggtttcagac
     5461 ggcggcgtac acagctcgct cgaccatttg tttaagctta ttgaaatctc caagctttac
     5521 ggcataaaga aatcctttgt gcattgcttt atggacggcc gggacaccga ccccaaaagc
     5581 ggtaagtcgt tcatagccgc tctgcaaaag cacctggatc gaaacacagg ggccatagcc
     5641 tctattgtgg gccgttacta tgccatggac agggacaaac gctgggagcg catcaaagaa
     5701 gcctacgatt tgctggtaaa gggcaaagga gtgaaagcac aagacatggt tcaggctgtt
     5761 caggcatcgt acgatcaggg agtaaccgat gaattcataa aacccattgt tcatacggat
     5821 gctcaaggcc aacctctggc caccattcag gacggcgacg tggtgctctt tttcaatttc
     5881 cgcaacgaca gagccagaga gctcaccata gtactgaccc aggaagacat gccacaacac
     5941 ggtatgcaga cgctctgcct ggaatactac accatgaccc cttacgatgc caccttcaag
     6001 ggcataaacg tacttttcga taaagaaaac gtcgacaata ccctgggtga aattgtggca
     6061 aaagccggca aaaaacaact gcgtatagca gaaaccgaaa agtacgctca tgtaaccttc
     6121 tttttctcgg gcggacgcga aaaagaattc gaaaacgaag accgcatact gataccatcg
     6181 cccaaagtgg ccacctacga cctgaaaccc gaaatgagcg ttaacgaagt gggcgacgcg
     6241 ctgaagcaag ccatagaaac cgaaaaatac gatctgattg tcgtaaacta tgccaatggc
     6301 gatatggtcg gccacacggg agtctattcg gccattcagg ccgccgtcgt agctgtggac
     6361 gccaatatta aaaaggttgt tgaagcagcc aaaagcaaag actacgaagt aatcataatt
     6421 gccgatcacg gcaacgccga caatgcgttg aatcccgacg gttctcccaa cacggcccat
     6481 tctctgaatc cggttccttt tgtgtacata agccggaata gttcagccga agtcagcgat
     6541 ggcatactgg ccgatgtggc gccctcgctc tgccacatcc tgggcataaa acagcctgcg
     6601 gaaatgaccg gacgctgtct tatacacgac taattacgga gtatgctgca aatcgataaa
     6661 accatcgtca gtctcgatat aatcaatcaa acgttttgct gcgacctgaa caaatgcaaa
     6721 ggcaggtgtt gtgtggaagg ggtgtcgggt gctccgctgg aagaagacga aattgccgaa
     6781 ctggaaaagg ctctacccgc agtatgggac gaactttcgc cccgagcccg tcaactcatt
     6841 aacgaacagg gagtttgtta caaggatgct tcgggcgaaa ccgttacttc catcgtagac
     6901 ggtaaagatt gtgtctttac ttattacgat gaaaagggcg tttgttattg tgctcttgaa
     6961 aaggcctgga gagaaggtaa aagctcattt atcaaacccg tctcctgcca cctctacccc
     7021 ataagacttt ccaaactgac ttccggagtc gttgctctca attaccaaca atggcatgtc
     7081 tgtgttcccg ccctggccaa aggaaaaact ttaggcttgc cgatttatcg ctttctcagg
     7141 gagcccttaa tccgtaaatt cggacaggac tggtacgatc agctctgcga agccgccaaa
     7201 atgctcgagg attatccccg ggatgtttaa ttaccgactc ctaccccttt tacggttatt
     7261 gttcggattt gttttttctc tgattttatc ccaggcataa gtgccggctt tttgccgttc
     7321 ggaactgttt atatccaacg ttttaaacga taaaatatgc acggtgaccc ccagagcgat
     7381 caagagtaat aaaattttaa gccaacaaat ttccagaaaa aaaagaactg caatcaggat
     7441 gcttatccac aacaaaacaa ccgagaatat tttggtgccc aaaggaatcg ccttatgttt
     7501 tctgtaattt tctatatagc gccctaaaca gggatgttgc atcagccagc ggtaataacg
     7561 atcggaaccc ttatagaaaa gagaagcgct cagcaacaaa aaaggcgtgg ttggtaaaag
     7621 cggcaaaaaa acacccagta tcgccaatgc caaaaacaaa actcccagca cgttatagac
     7681 aactttcatt cttttccgga attggagcac aaaagtagtt ttttacaccg agtctttaaa
     7741 attctggcag aatcctttat atttgctctg atcttaactc atttaaatca tgaaaaaaca
     7801 accgatcata ttatgttttt ttctgtggac agctttggct gccttcgcac aagagagaat
     7861 agacctgcct ttatggccgg atggagttcc cgaaagtaac ggcattactg ccgaagaaat
     7921 caacgaaaac aattattttt ccaacgtcag tgttccagcc atgtcggttt tcaaagctaa
     7981 tcccgacaaa aatacgggtg cggccctgat tatttgtccg ggaggaggct acgcccggca
     8041 ggctgccgga cacgaaggct ttcagctgac cggctggctc aacgaaaacg gcattaccgc
     8101 cataatcctg aaataccgtt tgcctaacgg acacgatttc attccttcta aagacgcctt
     8161 gcaggccatc agaatagtac gaagcaaggc cgaagaatgg ggaatcaacc cttccaaaat
     8221 aggtatttcc ggcttttcgg cgggcggcca tctggcttcg accgcaggca ctcacttcga
     8281 ttacggcaat cccgaagccg aagatcccat cgcccgtcaa agcagcaggc cggattatat
     8341 gatcctcttc tatccggtta tttccatgaa agagggtgtc acacacgtcg gttccaaacg
     8401 caacctattg ggtgacaacc caacgaaaga acaaatgttg gagtattcca acgaacttaa
     8461 ggtaagcgct gaaacaccgc caaccttgct gctgctcagc gacgacgaca aaggagtaat
     8521 accgcaaaac agccttgatt tttatgcagc gctaaaaaac cataaggtcc ccgctgctat
     8581 gtatattttc cccgaagggg gccacggctg gggctacaag gagagcttcc gctatcacca
     8641 tgtctggcgc atcctgatga tagaatggat gatgcagcaa aagattctgc ccgacatgat
     8701 caggcaacaa aattaaagag cttacctttc gactctgccg gcctgtattc ttaaatgttg
     8761 aatcagattg ttgccctgct gatcgtaaat actaagtgtg tgatttccgg gggcagggct
     8821 gatgctcatt tggtgtatcc tgcgtgtatt gcctatccac tcgtcgtcga gatgccaaaa
     8881 caaaagggca tctgctttgc ggtgtaccac ttcgattacc agcttgcccg gactgccgtc
     8941 aagctctctg gggatataca aactgtatgg attacgggga tacaatattt ccatctgctt
     9001 gtattctttt tcttcaccac agctttccat aaggggaggc aggccccggt aagacgggtg
     9061 agttttgcgg taaaaccact ccatagccgg cggtaacaca aagattgaat cactgcggat
     9121 aagttcaagc ggatagcaag aggcattcac cctgaatttt ccgtctttgg atacatgtac
     9181 cagccggtga taaggacata gttccgtttt tgagcctgat gcaggaacca tttgcaagct
     9241 cttccgggtg caataacccg aagctttaca gccactttcc atacatattt cttccaggtt
     9301 catctcgctg tcgggctgtt caaaccaagc tccgctctgc ggtaataaat caaaaagatc
     9361 aaacagggcg ggagcggccg ctctcaagcc tgtaagcccg ggacgtccct cgccgtcggc
     9421 attgcccgcc catacggcta ctacgtgttc gggagtagct cccagggccc aagcgtcgcg
     9481 taaaccaaaa ctggttccgg ttttccaggc aatccgccgg ctgctttcaa agtgcttcca
     9541 gccgctacgt tctcggggac gatttacttg tcgcaaggct tccatggtgc accatatgga
     9601 agcagccgac agtaaaggta aagattcctc gttttcggag gcggactttc ggcctttgct
     9661 aacagcattc ttccttttat tcgggaatat ttccggccaa aagcgactga tgtttgtctt
     9721 atgaaatgcc tttgtatcgc tctttttttc gggactattt tctctgaaaa gtcgtaaagg
     9781 acggtaatct tccgacgaat attcctgaac ggatccaaag cggtttagca ccctggccaa
     9841 agaagcataa gccccggcca attcgagcag gttgcattct cctccgccca aaatcagcga
     9901 caaaccataa tgctgggacg gataaacaaa agtggtgaaa cccaggtttt tcagaacatc
     9961 cagaaaacga gcttctccaa actgatgtag catttctaca gccggcacat taagggattg
    10021 gctcaaggcc tcagaagccg gaactgcccc ctggtagtta agatcaaaat ttttggggct
    10081 gaaacccgaa aaattcaccg gaacatcggc aataagagta ttagggagaa tttctccctg
    10141 atgcagcatc gaggcgtaca aaaatggctt aagaatgctt ccgctgctgc gtggggtctg
    10201 cacgatatcc acaaaaacat cgagttgagc caccgcgctg atgttgcccg cataggccag
    10261 aatactgttt tgctttacat cgcagaccag ggcagccaaa ttatggattt cactttgctc
    10321 cagttctttg ctccgctgtt ccagcaaagc gatgcatttt tcctgtagcg gataatctat
    10381 gcttgtatag acattttggc ccggaaactg tttatgggct ttttccacta catgcagggc
    10441 caaaccgggc aagggtagag gtgcctgcgg cagtgattcg gccaaagcca actgataact
    10501 aagactgtca atgtgttttt tttgataaag cttcccaagt aaagcatccc ttttttgttt
    10561 caattcggct gcgtttctgc cgggatgaat cagagcggga gcattaggca aaacggccaa
    10621 agtagctgct tcggcccagg acaacttttg atgcggatgg ttaaaataac gccaggaagc
    10681 agctgtgata cccaccacat tgccgccgaa aggagccacc ccggcgtaaa gagccaaaat
    10741 tttatctttg gaataacaca attcgagttt caaagccatc agaatctcga ggcattttcc
    10801 gctcagggtt ctgggaggat tattgcgaga cattctggcc acctgcatac tcagggtgct
    10861 tccgccactg acgatgcgtc cggctttgag attttgaaca agcgccctgc ccaacgaaac
    10921 cggattgacg cccggatgat aacgaaaata gttatcctcg taaagaatca gagccttgac
    10981 gtaattgtcg gggatttctc cctcggggct aaagcgccac tggccgtcag cggctattct
    11041 tgcacccaac agctccaaat tggcggcata aaccacagta gagcaggggt acttgaattt
    11101 aggaagaggt accagcaaaa acaacacaac aagcggcagg aacaagccgg taagtatttt
    11161 cagccgcttt gtcccttttg agctttggtt tttcaaacac attccctatg agcttgcccc
    11221 gttttagcgt tctactttta ccttagcccc gccttttctg gcgtaataag cctggtcgta
    11281 catggcttcg cacagcaccg gagccagaat aaagtcccca caataggagg cattcagctc
    11341 cagggtaaat accttcgttt tgccggcccc caacgaaaaa taagtattga ctctgtcgtc
    11401 gcgaatatcc ctgtaatcgt aattattttg atcctcgctt gcggacccgg taccaaaaag
    11461 cctctcgttg attatttccc agccggcggg tatcatttgg gtcaaagcca ggttatcgac
    11521 agcaaaagcc gtcttgttgc tgatgcgcaa ctgaacgatg aaattagttc cctgttttag
    11581 tttgctgaca ttcaactttt taccttcttg gtccatatag ctgatttcaa gggccaatcc
    11641 cttttcctgt tccgaggcat ccatccccgc tttgacaccc tggttgaaaa ggctggcaaa
    11701 aacagtgcct ttacccttgt tcaccaagcg taagtccagt tcaagatccc ggccatagga
    11761 tagatccacc agggtaaagg ctccggaagg ccgggtttgt tgcggcttcc cgtttacata
    11821 caactctgtc tccgcttcgg aagtcgctga ggttagcagg caaaattccg acaacacgga
    11881 aagaacaaaa ccggtggttt gcgtactgtg ccggcccgcc ctgctgaaat ccgtggctat
    11941 tttaagagcc agcggggcgg cctgttccca ctctcccaac aacatcaggc tttgcaacaa
    12001 ataacagcgg tcgcgcaaaa cggagccgta ggtataagcg ctgacggtgc ccggttcggt
    12061 gtcgcgcagg tctatcagtt tataagcggc ctctttcatc ccggaggcag cataagcaga
    12121 agccagcagc catttcccct ggttgttcag gcaatcggcc ttcattagcc ggttcatagc
    12181 tgccacctcg ggttcaccgg ccctggcaag aacaaacaag gcataggctt gaatctgggc
    12241 cttgtagtcg ggtaaatatt cccggtagcg gcgtaccccg tttgcaaggt agtcaaggcc
    12301 ggctctcttt aaattggagg gtattaaata agctgctttt tcggcctcga gcataaaata
    12361 agtcaggtaa gaagtcagcc acagttcgcc caccgaccgt ccgggccaga aggcaaaacc
    12421 tctgtccatg ctttgataac ggggaagacg atcgatacaa tcttttatgt tctgttcata
    12481 ttccttgcgt ttttcagcgc tcaattccaa cagtttcggc acataaatca agggaaaaac
    12541 agccgataca acctgctcgg cacattggtg agggtattgg ttgaggaaac tcaactgata
    12601 ctctaaattt agtggcaata ccgacgaaat ttccagtctg gccttattgg ttccgggtac
    12661 acccaaagca gacaaattcg cttgccagct ttcgcccgag ggcagcattt taaagacttg
    12721 ttccgtactg ggaggattgt tgtgcataac atccagctct atgctgtgtt gcgttttttc
    12781 tctgccggat actgcgctaa gctttatgat gcccaaacct gcttccccgg ctgctttaag
    12841 atcgaaaaag acatcctgtt gaccgggagt atcaaaaaga atcagggtat ctgcagaacc
    12901 ctggagtctg ccttccgttt caaaacgtat cctgactgtt ttgatggatt catccatggc
    12961 aaagaccgta aggggcagat cggctttctc gcctaaggcc agggttctgg gtaaactggc
    13021 atacaacatc aaaggttcgc tgacccggat gcttttttcc atgctcccgt aacgaccccg
    13081 gccggcagca atcagcatgg cccggacaga gcctgcgtag ggtttgagct gtattttatg
    13141 ctcggctttt tttccggcct gtagcctgaa agggcccaag acttctacga tggggacaaa
    13201 acggtcggct tttttcttgg agtaatctat ttcctgatcg gagcctccta tagcgtacat
    13261 gctttccaac cggctgccga atgaacccaa cacctcattg tacatatcat aagttttggt
    13321 tcccagggct tctttggcgt agaaatgagc ccagggatcg ggagtgctga aatttgtcag
    13381 atccagcaaa ccttcgtcca ccaacgctat ggtataatcc atggcttgtt tgttgctttc
    13441 gcttacccgg atggtatatt ctttttcggg gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnccatggc ttgtttgttg ctttcgctta cccggatggt atattctttt
    13621 tcggggcgaa gctcatcggg aatgtccagt accggatgca atcgggtctg ctcatcttcg
    13681 actctgaacg acgttagccc aaaaagcctg ataggcaggt cgttggctgt ttgcgcgtag
    13741 gcctgtatca gcataatgtt tacataaaca ttgggcgcca tgtcgggcgt agtaacaaaa
    13801 cggtattcgt tcatagcctc cagattgtcc agccattcct gatgcaggat gcctttgccg
    13861 ttttccagcg taaccagcgc ccgggcatcc ttgcctgccg gaaaactgag gctggctgtt
    13921 tctccggtct tatattgatc gcgctcaata accaaagcca acacctgagc gtcgcctttt
    13981 tgtataccgc tgccccaggg cagaccgaaa tagatttgct ggccgcaggt atgcccatcc
    14041 ggggccgtaa ccagcaataa ataacaacca tagcgctgcg aattgttgct cagttcaaaa
    14101 ctgccttttc ccttgttggt gctgattttt gtgctgtaaa ccggattatg ataagtttgg
    14161 ccggcgtagg aagccagcag attactgttg ctctgatacc accaatgata gtccaggtcg
    14221 tatagcttaa cttctatatt gctcaggtcc gccgggttgc ccttttcgtc aacactcact
    14281 acttcgaccc ggtggttttt atcattgtcc agttttttcc acgatggata ggaataattc
    14341 attttaaggc cgacaaaatg ttcgctggca ataaaaggaa tactgcgttg attgatactg
    14401 aaaccgccgc cctcgtacac tttggtgctg attctggcct gcatataacc ttcaaagtcc
    14461 ctaccaggac caaaagacat agttacggag gcttctcctt tgtagtcgag ctggccgtcg
    14521 aacaaagtga attctttgct tgattgccgg ggataaggag ttgaaaaatc gaaatccttg
    14581 tatttttcaa atgtgggtgt atagggatgc aatacggcgc tcacctgagc tttcatattg
    14641 gctgccggcg taccgtgcaa ccaggaggct ttgagttttg cccagcttgt ttcggattta
    14701 ctcaatacat cttcccttat ttcaagctca attttcaggc ggttaggttt tatgctttcc
    14761 acttgtatgc cgcgctgaaa aatatgggta ccggctttaa ccactacttt ccagttgccg
    14821 gtaggatcgt ctttgcttgt cgcaaaatag aaaggataaa tacagcgttc atcccgcagg
    14881 gtgagtattt tttttacaaa ttgcccccgg ggattgtaga tttccatatt tatgggatgc
    14941 ccttcgggta attgttgctc ccggtcttcg agtattaaat gcaggtatat gctgtcgccc
    15001 ggccgccaaa catcgcgctc ggcgtaaaaa aaagccttga tgccctcttc tgccttaatg
    15061 ccgtctgtat caaaattagc cacaggaatg gcgctgctgt tgtccagctt caagtaggca
    15121 tattggttac ccttttgggc aaacagcaca aagggagctc tttcgggagt aaaacggaaa
    15181 taaccctgtc cgtcggtttg agcacgggtc aggagctgat tctgataatc gtatacactt
    15241 aattgtactc cggcttcgga ggcggtagtc atcaaattac tcacgtaaat cgacatctgt
    15301 cccttagcgt ccatcttggc tatcaatgcc agatcggaac aaaatacgtt gcggttgaca
    15361 aaacgattag gcgagaaata cgcctgactg gccggatttt cgtagttacg ccaatcgtaa
    15421 tagtagttgt aataagtatt ttcatagctc tgacctggaa acaacatttc ctcatctata
    15481 ggcagataat gatccgtttc ttcggaagcc tgttcctcgt aaagcgaata agcttttcta
    15541 aagcccaact ctacattata gattgatccg ggttctaagt cgataaaaga agccagatcc
    15601 aggctgaaag cattccagtt ggtcaaatct tcggcccctt tgtcctcgag agatattttt
    15661 ttggtaacaa tggttcttcc cactctgttg agtccccatt tataagtctc atcgatgtcg
    15721 ttgtcctgga aaaaagccac tatattgttg ggaaaaatct ggataacacg aaggtcaacc
    15781 gctttgagcc ccgcagcaga aaaaggtaaa atcaggcctt gactctgagg catgatgacc
    15841 cctttgccca acaagcgtat ggccggcttt atacgcccga aattgatttt ttggctgtaa
    15901 tttttttgaa gtgtttttcc ctgtttattt tcgaccgaac gggatatatt cagagtaaac
    15961 tcccccgaca aggtattgga aggatagatt ttaaggacgt tgttatcgat ggtatactta
    16021 acactctttt gttcgtccag ggaaaccaat cctcttaaat cctgctgcat gttaagcaga
    16081 tcagaaaaaa cgatttcaag cacctgatcg gggctttctt ttaccttcac cgagctaagc
    16141 ataaaaacgt ctgtccccgg tatggtaaaa gaatattgac tgatggaagg tttataacta
    16201 ttatcaatag gcttgatatt cagcactaat tcgctgtttt ctttctccct tactatgttt
    16261 cgtatactta aggtgtggct gaatgtttcg ggattatgtt cccatttcaa gcttaactgt
    16321 tcatgcggat actcaacaat caggctctgc tctatctcgt gggaatcaaa acggtctgat
    16381 gtgttgatat atacctccag aaaataatta cctccctcgt ccagtcgtaa ctcatcataa
    16441 gataacttaa gttgcttttt tgcggtaaaa aaactgaaat tcaatttttt ttccagcttt
    16501 tcaggtaact tcagcaagta ttccaaattc aagcttgcct cataatttgt attccaattt
    16561 aagggttttt cgggtttaaa aaccaaagta ttaggtgatt tccaactaca ctttcctttg
    16621 atttcaggct taaagacgaa aggatttttt tcaagggtct ttcctatcaa ttccttttcg
    16681 actatgtctt tggtaaatac cacaacgata ggatctgccc gtcttatcgc cccggagctg
    16741 atggcagaaa taatgccgga ataatcactc aggtcttttt caaatttctt gtcttttcta
    16801 ttcccgcagg ctacagttcc gattgcaacg aggagaatga aagcaatttt gttaatctgt
    16861 ttcattttat cttttaattt tcatggagaa ctcacaacca caataatctt gtctgtataa
    16921 accgtattgt ttggataatt ctatagatcg tttgtagcct tctttctttt tgaaatcggc
    16981 atataaataa cgaacaccat atctattttc gtacactgcc cctaagtcat taataagttg
    17041 tgcatttttc aaaggactga tacttaatgt tgtggtaaaa tagtccaggg atttttcggt
    17101 cgctttacgg gcagcttcct ccaagcgtaa agcataacat ttttggcatc gacctcctcc
    17161 ctctttttcg ttttctaaac cttctatatt ccggtaaaat tcggaaggat cgtaatttgc
    17221 ttcttcaatg attggacgaa tttcagattt taattcccga acaaatttta ctaatgtcca
    17281 aagccgttta tcgtattctt ctttaggaaa tatattggga ttgtaataat aaagtgcaat
    17341 cgtaaaataa tcgcttaaat attccaacac ataggtacta caaggtgcgc agcatacatg
    17401 aagcaaaaga gttggttttt tattttcagc tttaatttct tccagtgttt tgtcgagaac
    17461 aagttgataa ttcatggctt gtataaacat ttctttacac acaaaagtaa ctgtttttac
    17521 taacttccgc ttggttttat caaaaatctg atatggatac aatgtatttt atatctttca
    17581 attgcccatt tcgcttaaaa attcagttat ctaatcgtgg ttttaaggaa tatgattccc
    17641 gaactgaata tcaacgcgaa cttttgcagt aattttctgc ttttgataaa aaagtagaaa
    17701 acttatttgt caacactaat atcattatta atttgaaatt tgaattttaa aacaaaataa
    17761 taaatgatta tagtctgttg atagtgtcga taatttaatt gaaataatct tattaacaat
    17821 agagcgaagt tataaagatc taatcaaata cttattaaca atgtaaataa ttgaacttaa
    17881 atcaaataat agatttatca acataataag cccgatcttg ttattttacc tgtcaaaaac
    17941 agacgttgtt caaaaccaaa ttttgtttgt tgaaaactaa tttataaata aaggatttat
    18001 aatttgcaaa attgagcaat tcgattaccc ggacgatttt tgataaaaca aaatttattt
    18061 atcaaaagct atccataatt attgacaaga tttcgacaga tatcgtattt ttgcaattgc
    18121 atcgaaacaa ccagagtccg tgtctaacaa tatttccaat acaccactga tgaaacagta
    18181 ttttagtatc aaggctaaat accctgatgc tgttttattg tttagagtgg gtgattttta
    18241 cgaaacctat ggaaaagatg ctatactggc cgccgaaata cttaatatag ccctcacctt
    18301 caaaactgcc ggtaagacag gaaaaattga aatggccggt ttcccatttc atgcattgga
    18361 tacttatttg cctaaattgg taaaggcagg caaaagagtg gctatttgtg atcaactcga
    18421 agatcctaaa aagactaaaa ccttggttaa aaggggtgtt actgaattag taacacccgg
    18481 agtttctacc aacgacgcag tattggatca tggtgaaaac aattttctgg cggctttaca
    18541 tctggatcaa aaaagagcag gtgtggcctt cttggatata tcaaccggcg aatttattac
    18601 agctgaaggc agttttgagt acgttgacaa attattgagt tctttcaatc ccaaagaaat
    18661 tttgtacgaa cgaagcaaac gctctctctt tcaagatact tttcattaca atggtccccg
    18721 ctttgagatg gaagattggg tttataccga cgattctgca caaaggcgct tattgaagca
    18781 atttcaaact gttagtctta agggttttgg tattcaaaac atgcctttag ccataatagc
    18841 ctcggcatcg gtgctttatt atatggatat gacccaacac ctacaattgg gacatataca
    18901 gcaaatttcc cgtattgacg aagataatta tgtacgttta gaccggtata ctatacgtaa
    18961 tcttgaatta tgcaatacca tcaatcagga aggcaaaagc ctgctggaaa ttctggacaa
    19021 aacctgcaca cccatgggaa aacgccagct gagacgttgg gtattatttc ctctgaaaga
    19081 cataaaagca attgaagcca gacaagaggt ggtagaattt ttcatgaaca acaaagaaat
    19141 tcaccaatat gtaaaagagc gtctgagtct ggtaggcgac ctggaacgta tagtgtccaa
    19201 agtggctgtg ggtagagtct cacctagaga agtcgtgcat ctgaaaaacg ccttgatgtc
    19261 ggtggccgac attaagcagt atctcaattc tcagtctttg gaaatcacac cgccattgat
    19321 gtcgatgatt caggctgtgg acacttgtga ggacctctgt ctgcgcatta caaccgaact
    19381 ggtcgaaaat cctcccatag ccctgaaccg gggcgaggta atacgtgccg gagtaaatga
    19441 agaactggac caattacgaa atttgtctac atcgggcaaa gaatacctac aaagactgca
    19501 ggaaagagaa agtcaaaaaa ccggcataag cagtttaaaa gtcagtttta ataatgtgtt
    19561 cggctactat atggaggtac gcaacgtgca caaggacaaa gtgcccgaaa actggatacg
    19621 caagcaaaca ttagtcagtg cagaacggta tattaccccg gagctcaaag aatacgaaga
    19681 aaaaatcctc ggagccgaag agcgcatatc tcagatagaa agcgatattt tcaatgccct
    19741 ggtcctggat ttagtcagtt ttgtaagcat tattcaaaac aatgccgtcc agctttccca
    19801 actcgattgt ttgctttctt ttgccgaagt ttcgagccta aacaattacc ataaacctca
    19861 attaagcgat tccgacgaaa tcaatattaa agccggacgc catcccgtca tcgaaaaaca
    19921 actgcccgat ggtgaaattt acataagtaa cgatgtgttt atggacaata aaagcgagca
    19981 gattctaatc ataaccggtc cgaatatggc cggaaaatcg gccttgatac gacagacagc
    20041 tctgattgta attatggcac agacgggttg ttttgtcccg gcagatgaag ctcaaatagg
    20101 attggtcgac aaggttttca ccagggtagg ggcctccgat aatctttcgc tgggcgaatc
    20161 tacttttatg gtagaaatgc tcgaagccgc cgatattttg aacaacatta ccccgcgcag
    20221 cctggtgctt ttcgatgaat tgggtcgggg aaccagcacc tacgacggca tttccatagc
    20281 ctgggctata gtggaatata ttcacgaaca gcccaaagcc agggcaaaaa cactctttgc
    20341 tacccattat cacgaattga acgagatgga aaaaaccttt ccccgcatcc gcaattacca
    20401 catttcggta aaagaagcag acaataaggt gatttttctt cgtaaaatgc gtcgcggcgg
    20461 cagcgaacac agctttggta tacacgtggc caagatggcc ggtatgccac ccagtatagt
    20521 gaacagagcc aatcaaatac tgcaagaatt cgaaacaagc caacagggga aagtgccggc
    20581 taagccggtt tccagactag ccgaaacccg ggagggtttg cagttgagtt tctttcagtt
    20641 ggacgatccg gtactttcgc aggtaaggga cgaaattctc aaagtggata taaacaactt
    20701 gactcctatt gaggcactca ataaattaaa cgagataaaa aagatagtca agggcagggg
    20761 ataataagcc tatacattga aacggaaata tataacatcg ccgtcttgca ctacgtagtc
    20821 tttgccttct acatgcattt tgccggcctc ttttacggcc tgttccgatc caaagcggat
    20881 aaaatcctcg tatttgatga cttcggcacg aataaaacct ttttcaaaat cactatggac
    20941 tatacctgca cagacaggag ctttgctgcc ggtcttaaaa ggccaggccc ggacttcgag
    21001 agggccggcg gttaaaaaca cactaaggtt caacaattta taagcagctt tgatcaggcg
    21061 gttcacgcct gattccttta gtcccagctc ttgcaaaaat aagctgcgtt cttcggcgtc
    21121 ttcgaattcg gcaatttccg attcaatttt agcagctacc accagcagtt ccgagccttc
    21181 ttcttgaatg gcctcgcgga ccatatccac atggcgattg ccttttagaa tggatgcttc
    21241 gtccacatta caaacgtaaa gcacgggttt gttggtcaac aaaaacaggt catgggcaat
    21301 tttctgttcg tctttggtgt caaattttac cgtacgggcc gacttgccct gttccagcgc
    21361 cagcttgtaa cgcagtagca cttcgtaagc tactttggct tgcttgtcgc ctccggtctg
    21421 ggcttgcttc tgaactttgt tgatgcgact gtcgatggtt tccaggtctt tgatttgcaa
    21481 ttcgtagtcg atgatttcct tatccctgac gggatctatt gctccgttta cgtgagcaat
    21541 attgctgtcg tcgaagcagc gtagcacgtg tatgatggca tcggtttggc gaatattgcc
    21601 cagaaatttg tttcccaggc cttctccttt gctggctcct ttgaccagcc cggcaatatc
    21661 gacaatctcg acggtagtgg gtactactcg cttgggattg accaatttgg ccagttcgtt
    21721 caaacgttca tcgggtactg tgataacacc gacgttgggt tcaatagtac aaaaagggaa
    21781 attggccgcc tgagctttag tattggacag acaattgaag agagtggatt tacccacgtt
    21841 gggcaaccct ataatgccgc attgaagtgc cataaactca aagtaaaaga agtcgcgaaa
    21901 gtacaaaaaa catcaatgcg cttcaagcca atttttaccc acgccaattt ctgccttgat
    21961 gggcactttg aacgaagctg cattttccat ttcgtaaata attatttttt taacttgttc
    22021 cagttcttcc tcgggcacga tgaaatcgag ttcgtcgtgc acttgcagaa tcatttttgt
    22081 ctttagctga gccgattcaa gggctaggtg gattctgacc atggctattt tgatcaggtc
    22141 ggcggccgtg ccttgaatag gcgcattgat ggcgttccgt tcggcatagg acctgacaac
    22201 ggcattgcgt gattgtatgt cgggcaggta acgtttgcga tgaaagaccg tttctaccca
    22261 attgttttca cgggccatct gcacagaatg atccatatag gccttgattt tgggataggt
    22321 ttcaaaatag tcttcgatca gctttttggc ttctgcacgt gaaacaccca ttctttctgc
    22381 cagaccgaag gttgaaattc cgtatataat cccgaaattg gcggttttgg ccttacggcg
    22441 catatcctga tcgacgtcct ctatgggaag cttaaatatc ctggctgccg tggccgcatg
    22501 aatgtcctgg tccagtctga aagcctctat catattctcg tcttcgctca ggtgggccat
    22561 tacacgcaat tcaatctgag aatagtcggc cgacaaaaac aactctccgg gaccagccga
    22621 aaaagctctg cgaatttctc ttcccttatc gtcgcgaatg gggatgtttt gcaggttggg
    22681 attgctggaa ctcagtctgc cggtggctgt tacggcctgg ttaaaactgg tatggatttt
    22741 tcctgttttg gggttgatga gcgggggtag ggcatcgatg taagtgctga gtagcttttt
    22801 taagcccctg tagttcaata ttttacttac aataggatgt ttgcttctca aggtttccaa
    22861 tacttcttcg gaggtggaat attggcctgt tttagtgcgt tttgctttct ccacaatacg
    22921 caagcgttcg aacaacacat ccccgacctg cttggcagag ttgacattaa aagtagttcc
    22981 tgccagaccg taaatttctt gttcaatacc gttcaattct tcggttagca gcaccgaaga
    23041 gtttttgagt gcttcagtat ctatatacac tccgctgttt tccatgtcgg cgagcacata
    23101 gatgagcggg gcttcaattt cgtaatacaa gttgttcagc cccccttgtt ctatctccgt
    23161 ttccaacttg cttttgagtt gcaaatagag atctgcctgc tcgcaaatga actcggccgc
    23221 catcttcaca ttcagatcct gttccgcact gagatcctgt tctgcgctga gattttgttc
    23281 cgcgctaaga ctttgttgca ccccgaggtc ctgttccacc cggaggctct gttccgcatt
    23341 cagatcttgc gactcaaccc gttttttcgc ttttagctcc ggtaagccct ctttgataag
    23401 acactggtag ttgaggtagc ttttggacaa aaaatcaaga tcgtgtctta attcgggttg
    23461 aataagataa tgcgcaatga ggatgtcgaa gaggggcccc gccaatgaaa gttcataatt
    23521 gcgcaagagc aatatttcgt tctttaaatc gtagccaatt ttaaggctgt gcttgttttc
    23581 aaaaagcggc ttgaaagaat tcaacaattc cttgcatttt tttctgttgt tggggcaggg
    23641 caggtagtag gctttgtttt tttcttttga aaaggcaatt ccggtcattt gagcattcat
    23701 agcctctgtt ccctgttttt ttgtgctaaa agcaaaaaaa tccgactcgg aaaattcttt
    23761 gataaaaccc ccaatatcct tctcattatc aagaatatgg taatagtgag ctgtgttttt
    23821 taagctgctg agaatcgatt ctggcaacga atctgtagac tcgtctggat ttattgccga
    23881 caaattgttc gaaaacaagt cgttccccac aatattttgc tgagtcttga tttttgttgg
    23941 taatgacgaa gaaccaacag cattaccctg ttcgtcccaa agtgaatttt gtaaatttaa
    24001 tccggcaggt ttttttttgc ttgctcccgg ttgagaagct tccacaaaaa ttggagtaaa
    24061 taattttttt tctccctcgg agtgacctga cggttttttt tcagagtcag cataagaatt
    24121 gctggcgctg attcttttca taagcgtccg aaattccaac tgctcaaaaa tgctcatta
//
LOCUS       DAOSTD010000024        21804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_2593_length_21804_cov_12.308014, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000024 DAOSTD010000000
VERSION     DAOSTD010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21804
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2593_length_21804_cov_12.308014"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3908)
                     /locus_tag="PLI37_02855"
     CDS             complement(<1..3908)
                     /locus_tag="PLI37_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOQ57992.1"
                     /translation="MEEKKKCLTSLGLLVAFCLSFVCLSAADYHFRQLTSDEKLSQNF
                     VDDIYKDSKGFMWFATWGGLDRYDGYSVISYNTENAKLKSNFCYCLAEDSHERLWVGS
                     DLGLSCIDLNTGRSLNLIEENAQYRNALSQTVYDLHCDSQSKLWVAGDHGLVCLVFDE
                     QGGIVNLYSLNMLTVFALCEDYDGRIWLATSSGKLLCLSNKNPGQFDFEIVPELLRRE
                     TFDFIRSIYSDRTGSLWVASSQGLFRYDPHADILQGYQHSETKSGSLPNNFVNDVLQD
                     NTGNIWVATLGGLALWNQETGNFTRFQSDDTDESINNNFLNSLFVDDRNILWIGTEKG
                     GVNWMFEKRKAIHGLRHDPANPGSIYPGPVNSILEDSRGNLWVGLVEGGLHFRARGQD
                     SFRHYLHNNKPGSLTQNTVSKIYEDSESTIWVGTWGGGLNRLRADGSGFKSYVPGDEK
                     GSIASLFISDLVEDSLHNGLWVGMNNGLQFLSKQSGQFLNVFVGSDIPYPPKQVNFLK
                     IDRRQRLWVATNFGLYCFYLHSFDIPGTPAKYDYFRFSLHDPSSGQLAKVISIYEDED
                     EDILWFGTNGDGLYKMEEISGADSADLKKKTGWEGQGVYKVAESYVRFVRYDKRHGFA
                     DNVIYTILEDDNDCLWMSTNNGLVRFEPQNTEVVNYSKEDGLLSNQFYWMAACKGHDG
                     TLYFGNLQGLNYIEPGRFKNNDYMPKPSLTSLQILNNTIEPGVAVNNKVYLEKHLYAS
                     SELVIPKKDKSFSLEFSALDYEFPECIRYSYYLEGFDKQWTDVGPERRFVYYTNLKQG
                     KYLFKLRCTNSDGVWSDKITGLPVVISPPFYQSLMFRLVLILVLLGLASFAFISREKR
                     IKQNRAELETKVKARTKELQEAYRQVEALNQEKLSVFTNITHEFKTPLSLIMGPIKKI
                     LNLSKDPLVLDQLKLAERNAENLLVLVNQLMDFRKVEEKASKLQKTRGNLLDLVREVL
                     LPFADAVERNFVQCSLISRLKNPELIFDAEILRKILSNLLSNAVKHTPDYGSIKIKLF
                     DIQKDNADWIRITVSDSGKGIPEEESEKIFERFYRIEDQETFRAAGQSGTGVGLFLCR
                     ELAQLHGGIIYAGNNRLGGARFVLEIPAEFPTSPVENLVKTENAVGDNVLDREEAEEG
                     RLRGNSSSAVVTPSKPLLLIVEDNKDMRQYLASVLREQYSLLEAADGEEGWTMVQRNL
                     PDVVISDVMMPRMDGIQLCRKIKTEFKTSHIPVILLTAFSSPNNEIEGLESGANDYLS
                     KPFEERLLKAKIKNILQLRERIHQSFAENMSTGQLEIAENS"
     gene            complement(4012..5721)
                     /locus_tag="PLI37_02860"
     CDS             complement(4012..5721)
                     /locus_tag="PLI37_02860"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ57993.1"
                     /translation="MKAFKQLCLGVLLLILPFVGGCDAMLDSGSERYTFEEDARLQTL
                     HDTLYNIVGIFSEMQKLGDRYVLLGELRADLMKVTDEASIDMKEIYYFNMSPDNAYAE
                     MKTYYSIINQCNYIIHYADTAYAEKSDKPLMRIYASAKSIRAWTYMQLVLNYGKVRFT
                     ELPILSVKEAEQDFPLIGMDALFTYLIDDLLPLKDIRLMEPGRFGNFDASRSIFPIPL
                     LLGDLYLWKGEYAAAAAMYYELIFEENLVINSQYIAEWGVENNAISTNTLSSAWFNSL
                     MLQNTEIVSALGAYPDYGHSFSLDSLSSQAKIMPTQIALDKWLNAVYYLNSSSTKNGD
                     LRIYDAVNNNYSMVKFGMITVDEADESVQELIKPTIRKFSQMNLSQSEYDPRHLPLYR
                     SSHVYLRYAEAINRLGYPQLAFAVMKNGLKLETMSLPHLVAEMAAVDSVIPSFMSFTY
                     AFFDNNAGTRMRGSRNVHEDETYFIIPDSITLVQQGYVNYGTAPSKQDTIDYVEDILL
                     EEMAMELAFEGNRFHDLMRIAYRRGDNAYLADRVAAKYEASEQAAIHDMLMVQDNWFI
                     PQE"
     gene            complement(5754..8912)
                     /locus_tag="PLI37_02865"
     CDS             complement(5754..8912)
                     /locus_tag="PLI37_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOQ57994.1"
                     /translation="MKRNSSYMSRIILSLGLLWVAVTLGSVKAADKAAESKLFVKGQV
                     LSQATGSPVFGATVRAGESSVFTNEEGRFELIIQTTNDVLVVTGDDFIAREYPLRGRQ
                     DVVIELYPRIFGENYKTVELPGGKTKSSKMISSAKTVDIGLNQAVSADELYHSSLGGD
                     IRTISRSGLPGIGSLLFIRGLNSLNAKAHPLLVVDGVIWNDISDVQSIFEGFQSNILM
                     NIDVNDIQSITVLKDGTSLYGSKAANGVVSIKTKRGESAVTKIDLRIMAGMVAEPGNI
                     PMMNADQYRVYASELLQTVSPGMLKTPKFLDFLIENPSDPIYNTYHNETDWSQEVYKR
                     GITQGYNISVNGGDQRALYHFSLGYTSNKGIVKTTEMERISSRFNADINVLDNLSVGM
                     NVGFSNIMRSLLDDGVNFYTSPTYMGYAKSPFLSPYSFTSLGYKAKDYDDADLFGIGN
                     PSSFIAGSQNKYKEYRFNVGFMPVYNINKHWTLSTLFDYSLNKYLEDYFSPMHGSYPR
                     ELEGFGMSENTVKNQLIRNTAIFDDTRLSYHLLKKNFEFKTLLGWRYMTNYLEADYIE
                     SHNSGTDNNTTVGNYDFLQTHGLNNRTKSVSTYANVDLAFLNRYFINAVLSLDGSSRF
                     GKEAKGGVELFGHSFALFPAFNAAWLISSESFMKNIRFIDYLKLRAGYDFTGNDGIQD
                     YATKAYFVTRRYIGQANGVILSNLANEELQWETTQSMHAGLDMTLFNERLLLNVDVFD
                     SNTDNLLTLRSLNQIAGVDYYWSNEGKLSNKGFEISAETKLVNTKNFQWSLGASIGHY
                     RNLVEELPEKGYTTPAYGGEIRTEVGQPAGVFWGYKTQGVYASEADALANPMAIVNDN
                     GSLTYFKAGDVIFEDFKKDGILDENDKQVIGDPNPDFYGNFSTKLSFKKWTLNTLFTY
                     SYGNDVYNYLRSQLESGKDFSNQTKAMLLRWTAEGQNATLPRSVYDDPMGNARFSDRW
                     IEDGSFLRLKSVSLSYTIPIENVFIKGLEFSLSANNLFLFTNYLGLDPESSVSDKVLF
                     QGIDNGLIPQTRSYFLGVKVNL"
     gene            complement(8936..10471)
                     /locus_tag="PLI37_02870"
     CDS             complement(8936..10471)
                     /locus_tag="PLI37_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOQ57995.1"
                     /translation="MKTNHSKLWPLIVLFLFALISCQEQAWYEHYEDSGKAGSEKTMM
                     EYIKSEPQLGLFLQMLEVSGYDTLLSVSQTFTVWAPQNNALSGIDLDDTTTVTEIISN
                     HIARYCIGTSSTRSDKVFMLSNKLVSFEGNKFGLTNLVSSNIKVANGLLHIIDGFVPY
                     VPNIWEYTGRIPGLDSLAAYFLSQNQREFNLLESKQIGVNADNQPIYDSSFIESNRYL
                     TERVNLNNEDAVFTALFPNNQAWHKSFDLIKDYYKTTDADGGWTKQKSLAQAALVKDL
                     VYLDNFKEKGIPDSLVTSSGHVIDSAKVLFQGAVSHDASNGVIYVTDSLRIPATSSWH
                     IPLQIEAEDNNYGRASLNAKLYNRTSAGTSVSASGGRYLEVEATGTTILSKTYVDFPI
                     PDVLSAKYDIYCLFVPESVVNRINPKPNVVNLYMSYLGPDGKLKNNVSIKKNVTIDAE
                     DTTLVHVTQMDFPYCSVLDRNSEISDLTVKLRVENAVTASQTRLYTRTMRIDCIIFIP
                     VED"
     gene            complement(10483..>11880)
                     /locus_tag="PLI37_02875"
     CDS             complement(10483..>11880)
                     /locus_tag="PLI37_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57996.1"
                     /translation="KAFELTGYRFIMDEAPIEDLTYDPKNYTPSGFRHTVLEELPKYR
                     KFGYTILAESDSTLANYTDCPKLPNGVKTLEDLYTLAEYFYSSKPGNIGLFSDGDEVK
                     DDYTDKRNYLNRYIAYHCMTRTLLSSRFIKDYDTPHHFKIYDMYEYIETMLEDRLLEV
                     KIDRNYSAPNSEFGFFNCKMAADGKEDASTGILLTDYKDNDAVNGYYHEIDKPLAYTR
                     EFEAALSAKRLRIEAACFFDELATNNMRGNAKWNPNGSNHGEPDKTHAYQIPFGYMDR
                     FFCTESTKFTYIGACSAYEDFQGDEMYARDSYNFTMQTLPIPAGTYEVRMGYQPTAYR
                     GIAQVYWDSIPTGIPVNFSLLASNPDIGHKVPGSEADDPYGYENDKMMRNRGYMKGPD
                     SYKCPVNIYSYSAANARYSTYNLRYILGTYEFKEPAKHYITIVFLGTESGDSQFMMDY
                     LEFCPTELILTEDTH"
     assembly_gap    11881..11980
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12139..13704)
                     /locus_tag="PLI37_02880"
     CDS             complement(12139..13704)
                     /locus_tag="PLI37_02880"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ57997.1"
                     /translation="MKNRLFIVSILALSLFATTACTNWLDTRPESEMILDEYWTTESE
                     AEAVLMAAYRGFITSDCTYRMLVWGELRSDNFTQGTGIGDEINKVLRQEITPSTPYTT
                     WSSFYSVINYCNTFLSFAPDVLKKDDNFTVEKLRKLSAEAYAVRALAYFYLVRAFQNV
                     PWISEPSLDDTQDFIVPQFSERAVLDSIIRDLELYSLPYIPRIHSTDKETKGRFTKNG
                     VYALLADIYLWDNQFEKCSQMCDYILADDYYTLVPAENFYSQVFYTGFSSESIFEFGY
                     DNNIQVNDATRSLYGYQNNQNGQLAFPVTLVSGQYSPFEYPAGSYIESKNDVDIRWKD
                     FFVNDPGVGVYQIFKYAGIQRTENFDGSRSTYSYRNNTSNWIVYRLPDVILMKAEAVL
                     QGKQEEGLAEALSLVNMTYSRSNGGDTLSLDYYDNSYDKLVELVLRERQRELMFEGKR
                     WFDLMRMARRAGSPAPLVSFIARKGEGSEALGKMSQMNALYWPVNKGELEANTALVQN
                     KFYETESTTSTLK"
     gene            complement(13717..16992)
                     /locus_tag="PLI37_02885"
     CDS             complement(13717..16992)
                     /locus_tag="PLI37_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOQ57998.1"
                     /translation="MKLFVKTMIALLLIGFPGLLRAQTEDIIIKGTVTNKANSSETLM
                     GVNIQELDVSNRVVGMSITDYNGQYVIRMKNANNKLVFTYLGFKKQTFEIGSLRVINV
                     QMEEEALELAEAMVTGQRMHTDGSGFVIPEREISTAMQTINAKEFEGLQVGTLDEALQ
                     GRVAGLDIVSTSGNPGSGSSMRIRGTASITGNKEPLIVLNGVPYEVNIKDFDFATSNE
                     EDYAELLSINPDDIEQITVLKDAASTAIWGSKGANGVLMVTTKKGQAGPTLVQYTYRF
                     TRSVQPKGLKMLNGDDYSMMIKQAYFNPRQDESAADIDEFKYSDTFVEYENFNNNTDW
                     IKAVSQTGRTHDHYLNVSGGGERATFRVSGGFYDQTGTIIEQHLNRFTSKAYLDYAVS
                     ERLKFISDFSFTHVDNQRNYSNLLGTAYAKMPNVSIYEQDRDGNDTDVFYNISRNSRL
                     NAAQRDMINPVAIAKLGVNNLKSYRIVPIFQLHYNLTGERSRSLRYQMYVSFDINNNK
                     NSGFLPWEATNNTWNSNQVNRSESNDSESLSVLTENVINWQPKLRNEDHSLTLYGALQ
                     TRTGNSGSQYIASYGLFSDKTKDASNEAYLEGVSTGRSNWRSMGLVGRVHYTYKSRYI
                     ISGVVRRDGSTKFGNKRKFGNFPSLSLKWIISDEDFMADTRSWLSMLAIRPNWGMSGR
                     EPGAEYLHFSRYAAYDSYLGKQATRPVSLRLSDLKWATLREWNIGADIGLFRDKIVMD
                     MNYYHRRTVDMLFSNISLPSTSGFSSMSYANAGTMDNDGWEFNVYTNRLVRRGDFSLD
                     VNFNISNQVNTIIELDDRILANYNKDYDFRNGTYMTRLQEGHSFGSIYGFEYEGVYQY
                     DKYDEEHGHLDAPVARDKDNRVITDETGEPLPMVFAAGTTVEYEFRGGDAKYKDQNHD
                     GSIDELDIVYLGNCNPLFNGGMGFTLRYKTLSCTAFFNYRYGNKVVNAARMNAENMYD
                     NDNQSIAVNWRWRKDGDITGIPRALYNYGYNWLGSDRFVEDASFLRFKYLTFNYILPS
                     NWLKPIGIDRVSAYLTFNNLYVFTKYSGMDPEIGYGDFGISTDNAQTPRSKDFTLGLT
                     IGF"
     gene            complement(17040..19670)
                     /locus_tag="PLI37_02890"
     CDS             complement(17040..19670)
                     /locus_tag="PLI37_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ57999.1"
                     /translation="MNLMKEFKNPFRLLMRLSVVFGLVFLVSCKDDYIYDEKSPDWLG
                     ESIYDYLKQDGHFDYYVKIIEDVGQKEVLAKTGSKTLFVTPDSIFDRFFQDNVWGLNS
                     FDEMNLSQKRQLLFFSMIDNAYLIETLSNYYSGGVLNEGQAMRRLTGLSVLDSVPFIE
                     GNKLPKATVWNRWRTKGMYLLKDNSTKPMVHFLQKYLENVGISDQDFKVMTGADRSYN
                     DAFIFQHKVIKRDIVCKNGYVNVLDGVMIPRDNMAEYIRSNENITQFSELLDRFCAPY
                     HTPGLTTTYNNLHPEATIDSIFYLRYFAPTNQGGVTSYPNGSKIPDDQLLPYDPGWNS
                     YAAGGAALQSDMGVIFAPTNEALDYYFNEGSGRELKKRYGSWDGVPNDILVLLITRHM
                     RKSLVNSFPGLFHKMVDEASSPLNVLNSDIVDSLNYVGVNGLVYVTSSIYPPDDFVSV
                     YSPVLFSEKTLVLDWGIKRYLYRLYLNSMVSKYAFVIPTDKALERYIDPYTYSSNYPT
                     VLKFWFNRLTSSVNVTVFNYDKVNDVVLDSVTTLNNANFIRNRLTRILDQSIVVGDFK
                     EDQDDAVYRDGYYVTKDGNILYADGTADIDGSKLTSNLVNFSAGADIENGTQIHLLDS
                     GIFYQKNGTSFMVDQLPQTPLQSFYTVLSDSAKYPEFDAFFSYCENFPGIFEAGRGSR
                     HFMDFNVTFFNTYRYTVYVPSNAAMEEAFNSGLIIPWDSIAKIESTAEYNAVVDSMQR
                     FIRYHFQDNSLFIHPNQVIEPARYYTATIKNDDSESYFNTYKNKFYRIQAEGDGTGIT
                     LTTEYIDKVNNKPYTAKVDVNARTPDGRTYYNIMTRDYVFNSNPKNLTGLTTSAYSSS
                     EVTTSSTAVVHLIDRVLRYR"
     gene            20082..>21804
                     /locus_tag="PLI37_02895"
     CDS             20082..>21804
                     /locus_tag="PLI37_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOQ58000.1"
                     /translation="MKKNYLKLATRTCLLAITCMLATSALADITSNLHLHYTFDGLTA
                     EQSEVPDVSGNNNAGTRMGGAYYVEGKFGDALRLETVNDYVYMPDGFMVGMEDITIAG
                     WIYKDAANGWDRLVDFATNTDTNMFITTGGPGYVRFAIKNIQVRPAGEEHINGKALTF
                     GRWNHIAVTIKYTNNIGLGILYVNGIEVGRNNNITLKPSDLGFTTKNYVGKSVYTADP
                     SVVGNIDDLRIYKRALTADDIAELLGVPDEITDCYNNLALDLPEDYEFTGLTADLTLP
                     TTWADSAVSISWTSSDTLLIDNEGKIVAFPEKYQDACILTASLLQMRGEEEFTAERTF
                     IVKVYPQETEVNPIIAVWDFTEETLGTDEDGNVTVTDKSGNDFVGTCLGGARIVTMGE
                     SNPVSAMYIEQAGAYFDMGQEIGKAVAGLTDYTISVFYRKDSVGGVTWSSSAYGQWLY
                     CFCNSNDLASDGQGGIFYEPYRAYHAVFTTNYGDETGVQRGQRRTPIGTWHNITYVQS
                     GQKGTLYFDGEVAGSSDNVYIPAYALKAKGHRGTIHNYIGLPCYSGDPKLSKTHIAGL
                     QIYGVALT"
BASE COUNT         6018 a         4998 c         4630 g         6058 t
ORIGIN      
        1 gaattttcgg caatttccaa ctggccggtg ctcatattct cggcaaaact ttggtgaatg
       61 cgttcgcgaa gttgtaggat attctttatt ttcgccttaa gcagacgttc ttcaaaaggc
      121 ttggacaaat aatcgttggc tcccgattcc aaaccttcta tctcattgtt cggagagcta
      181 aaagccgtta ataatatgac gggtatatga gatgtcttaa actctgtttt aattttgcga
      241 cacaattgta tgccgtccat acgaggcatc ataacatcgc ttataactac atccggcaaa
      301 ttgcgctgca ccatcgtcca gccctcctcg ccatctgcgg cttccaacaa gctatactgt
      361 tcccgtaaaa cagaagccag gtactgacgc atatctttgt tatcttccac tatcaacaac
      421 agaggcttcg atggagtcac aacagcggat gagctgtttc ctctaaggcg cccctcctct
      481 gcctcctccc ggtcgagaac gttgtcacca actgcatttt ctgtctttac taagttttca
      541 accggacttg ttggaaattc tgctggaatt tccaacacaa aacgggcccc gcccaagcga
      601 ttgttgccgg cataaatgat acccccgtgc aactgagcca gttctctgca caggaagagc
      661 cccacccctg ttccggattg tccagcggcc cggaaagttt cctggtcttc gatccgataa
      721 aaacgttcga atattttctc cgattcttct tcgggaatgc ctttaccgct gtcgctgacc
      781 gtgattctta tccagtcggc attatctttt tgtatatcaa aaagctttat tttgatactg
      841 ccgtagtcgg gagtatgttt caccgcattc gacagtagat tactcaggat tttacgcaga
      901 atctcggcat caaaaataag ctccggattt ttaagcctgg atatcagcga acactgaacg
      961 aagttcctct cgacagcgtc ggcaaaaggt aataacacct cccggaccaa atccagcaaa
     1021 tttccccggg ttttctgcaa tttagaggct ttttcctcta cctttctaaa atccatcagt
     1081 tgattgacca gcaccagcaa attttcggca ttgcgctctg ccaacttcaa ctgatccagt
     1141 accagaggat ccttgctcag gttgagtatc ttttttatag gccccataat caaacttaaa
     1201 ggcgtcttaa actcatgagt aatgttggta aacaccgata acttctcttg gttcagagcc
     1261 tccacctgcc ggtaagcttc ctgaagttct ttagttctag ctttaacctt ggtttcgagt
     1321 tctgcccgat tttgtttgat cctcttttct ctgcttataa acgcaaacga agccaatcca
     1381 agtaacacaa ggatcagtac caagcgaaac attaggctct gatagaaggg aggacttatt
     1441 acaacaggca gaccggtgat tttgtccgac catactccgt cgctgttggt gcacctgagc
     1501 ttaaacagat atttgccttg tttaagattg gtataataga caaaacgacg ttccggcccc
     1561 acatcggtcc attgtttgtc aaaaccctcc aggtaatagg aataacggat acattcagga
     1621 aactcataat ccagagcgct aaactccagc gaaaaagatt tatctttttt tggtatcacc
     1681 aactcagagc ttgcatacaa gtgtttttcc agataaactt tgttgttgac agccacgccc
     1741 ggctctattg tattgttgag aatttgtaag ctggtcaagc tgggtttagg catgtagtcg
     1801 ttgtttttga agcgtccggg ttctatgtaa ttcaaacctt gtaaattacc aaaatacagt
     1861 gttccatcat gacccttaca agccgccatc cagtagaatt gattactcaa caagccatcc
     1921 tctttggaat aattgaccac ctcggtattc tgaggctcaa agcggaccaa tccattattg
     1981 gtactcatcc ataaacaatc gttgtcgtct tcgagtatgg tatagattac attgtcggca
     2041 aaaccatgtc ttttatcgta acggacaaaa cggacataag attctgcaac cttatacaca
     2101 ccctgacctt cccagccggt ttttttcttt aggtccgcac tgtctgctcc acttatttct
     2161 tccattttat acaggccgtc gccgttagtt ccaaaccaca agatatcttc atcttcgtct
     2221 tcgtagatac tgattacttt ggccaattgc cccgaggaag gatcgtgtaa actaaatctg
     2281 aaatagtcgt attttgccgg cgttccggga atatcgaaag agtgaagata aaaacaatac
     2341 aagccaaagt tggtagccac ccacaaacgc tgcctacgat cgattttcag aaaatttacc
     2401 tgtttgggcg gatagggaat gtctgagcca acaaatacat tcagaaattg tccgctttgt
     2461 ttagataaaa actgcaaacc gttattcatg cccacccata aaccattgtg caggctgtct
     2521 tcgaccaggt ccgaaataaa aaggctggca atactgcctt tttcgtctcc cggcacatat
     2581 gatttgaaac cggaaccatc ggcacgcagg cgattaagtc ctcctcccca ggttcccacc
     2641 catatagtgg attcggaatc ctcgtaaatt ttggataccg tattttgcgt aaggctgccc
     2701 ggcttgttgt tatgcaggta atgccgaaaa gaatcctggc ccctggctct gaaatgcagc
     2761 cctccttcga ccaggcccac ccaaagattg ccccggctgt cttcgagtat ggaattgacg
     2821 ggcccgggat aaatacttcc gggattggca ggatcgtgac ggaggccatg tattgccttt
     2881 cgcttttcaa acatccagtt aactccgccc ttttcagttc caatccacaa aatgttacga
     2941 tcgtctacaa aaagagaatt cagaaaattg ttgtttatgc tttcgtccgt gtcgtccgac
     3001 tgaaaacggg taaaattgcc ggtttcctga ttccacaaag ccagaccgcc cagagttgct
     3061 acccagatat tgccggtatt atcctgcaaa acgtcgttta caaaattatt aggtaaagat
     3121 ccggattttg tctccgaatg ttggtagcct tgcaggatat cagcgtgagg atcgtaacga
     3181 aacaagccct gggaactggc aacccagaga gagcccgttc tgtcggaata gatactgcga
     3241 ataaagtcaa atgtttcgcg ccgtagtaat tccggtacaa tttcgaaatc aaattggccg
     3301 ggattcttgt tggagaggca tagtaacttc cctgaagaag tagccagcca gatacggcca
     3361 tcgtaatctt cacacagagc gaagactgtc aacatattta aactgtatag attaacgatg
     3421 ccgccttgtt catcaaatac caggcaaacc aggccgtgat ctccggccac ccagagctta
     3481 gattgtgaat cacagtgcag atcgtaaacc gtttgggaaa gagcattccg gtattgcgca
     3541 ttttcttcta ttaagttcaa tgaccggccg gtgttcaaat caatacagga caagcccaaa
     3601 tccgatccca cccagagccg ctcatgactg tcttcggcca aacagtaaca gaaattactt
     3661 ttaagttttg cgttttcggt gttgtagctt ataacggagt aaccgtcgta acgatccaaa
     3721 cctccccagg ttgcaaacca cataaagccc ttgctgtctt tatagatatc atcaacgaag
     3781 ttctgagaca gcttctcgtc ggaggtcaat tgtctgaaat gataatcagc cgccgataaa
     3841 catacaaagc ttaagcaaaa tgcgacgagc agacccaggc ttgtcaagca tttcttcttt
     3901 tcttccatgc aaaatgtctt tcgatagaca aaatcgcaac aatgatacaa aaaagaagcc
     3961 agatagccta agtaggactt aatagattcc tccctctata aatgcaaacc gtcactcctg
     4021 cgggataaac caattatcct gaaccatcag catgtcatgg attgctgctt gctccgaagc
     4081 ttcgtattta gcagccaccc ggtcggccag ataggcattg tcgcctctcc ggtaagcaat
     4141 acgcatcagg tcatgaaaac gatttccttc gaaggccagt tccatcgcca tttcctccag
     4201 caatatgtct tcaacgtagt cgatggtatc ttgtttggaa ggagccgttc cgtaattgac
     4261 gtagccctgt tgtaccagag tgatggagtc gggaataata aaataagttt cgtcttcgtg
     4321 cacattgcga ctgcccctca ttcgtgtacc ggcgttatta tcaaaaaagg cataggtaaa
     4381 cgacataaat gaaggtataa cactatcgac tgcagccatt tcggccacta agtgtggcag
     4441 actcatggtt tccagtttca agccgttttt cataacggca aaagccagtt ggggataacc
     4501 caggcggttt atagcctcgg cataacgcaa atacacgtgc gaactacggt acagaggcag
     4561 atgtctggga tcgtattccg attgggacaa attcatctgc gaaaatttcc gtatagtagg
     4621 ctttatcaat tcctgtacag attcgtccgc ctcgtcaaca gtaatcatac caaatttaac
     4681 catggaataa ttgttattga cagcatcgta aatgcgcaaa tccccgtttt tggtagaaga
     4741 cgagttcagg taatacacgg cattgagcca tttgtcgagc gctatctgcg tcggcataat
     4801 tttagcctgt gagctcaggg aatctaggga aaaactatgt ccataatcgg gataagcccc
     4861 tagggccgaa actatttcag tgttctgcaa catgagcgag ttaaaccaag cggatgaaag
     4921 tgtgttggtg cttattgcgt tgttttccac tccccactca gcaatgtatt gagaattaat
     4981 aactaaattc tcttcaaaaa tcaactcata atacatggcc gctgccgccg catactctcc
     5041 tttccaaaga tacagatcgc ccagcaacag gggaatggga aagatggagc gcgaagcatc
     5101 aaaatttccg aacctaccgg gctccatcag gcgaatgtct ttgagaggca gcaaatcgtc
     5161 tatcaaataa gtgaaaagag catccattcc aatcagcggg aaatcttgtt cggcttcctt
     5221 aaccgaaaga ataggcaatt cagtaaaccg caccttacca taattcaaaa ccagttgcat
     5281 ataagtccag gctcgtatag acttggccga agcgtaaata cgcatcaagg gcttatcgct
     5341 tttttcggca taggcagtat cggcgtagtg tatgatatag ttgcactgat taataatgga
     5401 ataataggtt ttcatttccg cataggcatt atcgggcgac atgttgaaat aataaatttc
     5461 tttcatgtcg atcgaggcct catccgtcac tttcatcagg tcggcacgca gttctcctag
     5521 caatacatag cgatcgccca gtttttgcat ttccgaaaaa atgccgacta tattgtaaag
     5581 tgtgtcgtgt aaggtttgca aacgggcatc ctcctcgaaa gtatatcttt cggaaccgga
     5641 atccaacatg gcatcgcaac cgcccacaaa gggcaggatc aacagcaaca cacccaggca
     5701 taattgtttg aaagctttca tttttcttgt catattaata tgagtgatag taattagaga
     5761 tttaccttga ctcccaaaaa gtaacttcgt gtttgaggaa tcaaaccgtt atctatgccc
     5821 tgaaacaaca ccttgtcgct gaccgaactt tcgggatcca gacccaggta attggtgaac
     5881 aaaaacaggt tgttggcgga aagcgagaat tcaaggcctt tgataaacac attctcaatg
     5941 ggaatggtat aagaaagtga aacggattta agccgcaaaa acgaaccgtc ttctatccag
     6001 cgatccgaaa aacgggcatt ccccatagga tcgtcgtata cgcttctcgg caaagtggcg
     6061 ttctgtcctt cggccgtcca tcgcaacaac atagctttgg tctggttgct aaaatcttta
     6121 cccgattcca actggcttct caggtaatta taaacatcgt tgccataaga ataagtaaac
     6181 aaggtattta aggtccattt cttaaaactg agtttagtcg aaaaattgcc gtagaaatcg
     6241 gggttgggat ctccgatgac ctgtttgtcg ttttcgtcca atatgccgtc ctttttaaaa
     6301 tcctcaaaga ttacgtctcc ggctttaaaa taggtcagtg aaccgttgtc gtttacgata
     6361 gccatgggat tagccaaagc atcggcttcg gaagcgtaaa cgccctgagt tttgtaaccc
     6421 cagaaaactc cggcgggctg tccgacctcg gttctgattt cgccgccata agcaggagta
     6481 gtatagcctt tttcgggcaa ctcttcgact aaattgcggt aatgccctat gctggctcca
     6541 aggctccact ggaagttttt agtatttacc aatttggttt cggctgatat ttcaaaaccc
     6601 ttgtttgaaa gtttaccttc gttgctccag taataatcga caccggcaat ctgattcagg
     6661 cttctgagtg ttaacaaatt atccgtattt gaatcgaaca cgtccacatt cagcagcaag
     6721 cgttcgttaa acaaagtcat atccaaaccg gcatgcatgc tttgagtggt ttcccattgc
     6781 agttcctcgt tggctaaatt actaagaata acaccattag cctggcctat atagcgtctg
     6841 gtaacaaaat aagccttggt agcataatcc tgaatgccat cgttgccggt aaaatcgtag
     6901 cccgcgcgca acttaagata atcgatgaac cggatgtttt tcatgaacga ttcggacgaa
     6961 atcaaccaag ccgcgttaaa tgccgggaac agggcaaaac tgtgaccaaa gagctcaaca
     7021 ccccctttgg cctctttgcc gaaacgcgaa gatccgtcca gcgagagtac agcattgata
     7081 aaatacctgt tcaggaatgc caaatcaacg ttggcataag tcgaaaccga ttttgtacga
     7141 ttattcagac cgtgggtctg aagaaaatcg taattcccta cggtagtatt attatctgta
     7201 cccgaattat gcgattcaat atagtcggct tcgagataat tggtcatata acgccaaccc
     7261 agcaaagttt tgaattcaaa attctttttg agtagatggt aactcaagcg ggtatcgtca
     7321 aaaatggccg tattgcggat caactgattc ttgacggtgt tttcggacat accaaaacct
     7381 tcaagctcac ggggataaga accgtgcatg ggactgaaat agtcctccag gtatttgttg
     7441 aggctgtaat caaaaagcgt actcaaagtc caatgtttgt tgatgttata taccggcata
     7501 aagccgacat taaagcggta ctctttgtat ttgttttgag aacctgcaat aaatgaagag
     7561 ggattaccta taccaaataa gtctgcatcg tcgtaatcct ttgctttgta gcccaaggaa
     7621 gtaaaggaat aaggacttaa gaaaggagat ttggcatagc ccatgtaagt gggcgaagta
     7681 tagaagttga ctccatcgtc caacaggctg cgcattatgt ttgaaaaacc gacgttcatg
     7741 ccaacgctta gattgtccag tacgttgata tcggcattga accgggaact tattctctcc
     7801 atttccgttg tcttgactat acccttgttg ctggtataac ccagggaaaa atgataaagt
     7861 gcccgttggt cgccaccgtt cacactgatg ttgtaaccct gcgtgatgcc tcgcttatac
     7921 acttcttgcg accaatccgt ctcattatga taggtattgt agatgggatc ggaaggattt
     7981 tcgatcaaaa aatccagaaa tttgggcgtt ttcaacatac ccggactgac ggtttgcagc
     8041 aactcgctgg cataaacccg gtattgatcg gcgttcatca tcggaatatt tccgggttcg
     8101 gcaaccatgc cggccataat gcgaaggtca attttagtaa cagcactctc accccgcttg
     8161 gtttttatac taacgactcc gttggcagcc ttgctcccgt agagagacgt accgtctttc
     8221 aataccgtga tgctctgaat gtcgttcaca tcaatattca tcaaaatatt ggattgaaag
     8281 ccttcaaaaa tggattgaac atcggaaata tcgttccata ttacaccgtc aacaaccaac
     8341 aacggatgtg ccttggcatt cagagaattc aaaccacgga taaaaagcag gcttcctatg
     8401 ccgggcaaac cggaacgact gatggtacgt atgtctcctc ccaatgagga atggtagagt
     8461 tcatccgctg aaaccgcttg attcaaccct atatcaactg tcttggccga agaaatcatc
     8521 ttcgagcttt tagtttttcc tccgggaagc tccactgttt tatagttttc gccaaatatt
     8581 cgggggtaaa gctctatgac aacgtcctgc ctgcctctga gaggatattc gcgggcaatg
     8641 aaatcgtctc cggtaacaac caaaacatcg ttggttgttt gaataatcaa ctcaaaacgc
     8701 ccctcttcgt tagtaaacac agacgattct ccggctctga cagtagcgcc gaaaacggga
     8761 ctacccgtag cctggcttaa tacctggcct ttcacgaaga gcttgctctc tgcagcttta
     8821 tctgctgctt ttactgaacc taaagttact gcgacccaca aaagacccag tgataaaatg
     8881 atcctgctca tgtaactgct atttcttttc ataatacatt cgtgctttta caggtttagt
     8941 cttcgaccgg tataaaaatt atacaatcta tgcgcatcgt tcttgtatac aatcgcgtct
     9001 gactggcagt aaccgcattc tcaaccctga gtttaacagt taaatcgctt atttcggaat
     9061 tgcgatccag tacgctgcaa taggggaaat ccatctgtgt cacatgtaca agggtggtat
     9121 cttctgcatc gattgtcaca tttttcttta tcgaaacatt gtttttcaac tttccgtctg
     9181 gccctaaata cgacatatac agattgacca cattgggttt cggattgatg cgattcacaa
     9241 cgctttcggg cacaaacagg cagtatatat cgtattttgc cgacaaaacg tccggtatgg
     9301 gaaaatccac ataagttttc gacaagatgg ttgtgccggt ggcctccact tccagatacc
     9361 tgccgcccga agccgacaca gaagtacctg cgctggttcg attatacaac ttggcattga
     9421 gagaagcccg gccgtaattg ttgtcttcgg cctctatctg taaaggaata tgccaagaac
     9481 tggtggcggg tattctcagg gaatccgtga cataaattac cccattacta gcgtcgtggc
     9541 taactgcacc ctgaaataaa actttggcac tgtctattac atggcccgac gaagtcacaa
     9601 gagagtccgg aattcccttt tctttgaaat tatcaaggta aaccaagtct ttgactaaag
     9661 ctgcctgagc cagcgatttt tgtttagtcc aacccccgtc ggcatcggta gttttgtaat
     9721 aatccttaat aagatcaaaa gacttatgcc aggcttggtt gttggggaaa agtgcagtaa
     9781 agacagcgtc ttcgttgttt aggttaaccc gctcggttaa ataacggttg ctttcaataa
     9841 aagaagagtc gtaaataggc tggttatctg cattgactcc tatctgttta ctttccagca
     9901 gattaaattc tcgctgattc tgactgagga aatacgcagc cagagagtcc aaacccggaa
     9961 tgcgacctgt atactcccag atattcggca cataaggaac aaaaccgtcg attatgtgca
    10021 agaggccgtt ggctactttt atattggagc tgaccaaatt tgttaaacca aacttgttac
    10081 cttcaaaaga aaccagttta ttgctgagca taaacacctt atcgctcctg gtagaagaag
    10141 tacctatgca gtagcgggct atatggttac tgattatttc ggtaacggtg gtggtatcgt
    10201 cgaggtctat ccccgacaag gcattgtttt ggggagccca taccgtaaaa gtttgagaaa
    10261 cggacaacaa cgtatcgtaa cccgaaacct ccagcatttg taaaaacaga cccagttgcg
    10321 gttccgattt tatatattcc atcatggtct tttcagatcc ggcttttccc gaatcttcat
    10381 agtgctcata ccaggcttgt tcctggcaag atatgagcgc gaacaggaat agcacaatca
    10441 agggccataa tttcgaatgg ttggtcttca tataatttat gtttaatgtg tatcttcagt
    10501 aagtatcaac tcagtggggc aaaattccag gtaatccatc ataaattgac tatcgcccga
    10561 ttccgttccc aaaaagacga tggtaatgta gtgtttagcc ggctctttaa attcgtaagt
    10621 tcccaggatg taacgcaggt tataggtgct gtaacgtgca ttagcggcac tataagaata
    10681 gatgttgacc ggacatttat aactgtcggg gcctttcatg tagccacgat ttcgcatcat
    10741 cttgtcgttt tcatagccat aaggatcatc cgcttcactg cccggaactt tatgtcctat
    10801 atcgggatta ctggccaata aagaaaagtt cacaggaatg cccgtgggaa tactatccca
    10861 gtacacctgt gcaatacccc ggtaagccgt aggctggtaa cccattcgca cttcgtaagt
    10921 tccggcaggt atgggcaaag tctgcatagt gaaattgtaa gaatcgcgag cgtacatttc
    10981 gtctccctga aaatcctcgt aagctgaaca ggctccaata tatgtaaact tggtgctttc
    11041 ggtgcaaaaa aaacggtcca tgtagccaaa aggaatctga taagcatggg ttttatcggg
    11101 ttctccgtgg ttacttccat tggggttcca tttggcattt cccctcatgt tgttggtagc
    11161 cagttcatca aagaaacagg cggcctcaat gcgaaggcgt ttcgcggaaa gagcggcctc
    11221 aaactctctg gtgtaagcca gaggtttgtc aatttcgtga taatacccgt ttacggcgtc
    11281 attatctttg taatcggtca acaaaatgcc ggtagaagca tcttctttgc cgtcggcagc
    11341 cattttacag ttgaaaaaac cgaactcgct gttgggggcg ctgtaattac ggtctatctt
    11401 tacctcaagc aaacggtcct ccaacatagt ctcaatgtat tcgtacatgt cgtagatttt
    11461 gaaatgatgc ggagtgtcgt aatctttgat aaaacggctc gaaagcaggg tgcgggtcat
    11521 acagtggtaa gcgatatacc tgttcaggta atttcttttg tcggtataat cgtctttcac
    11581 ctcgtccccg tcggaaaaca gacctatgtt ccccggcttg ctggaataaa agtattcagc
    11641 cagagtatac aaatcttcca gcgtttttac accgttgggc aatttgggac aatcggtata
    11701 attagccaga gtggagtcgc tttcggccaa gatggtgtat ccgaatttcc ggtatttcgg
    11761 taattcttcc agcactgtgt gacgaaaacc gcttggagta taatttttcg gatcgtaagt
    11821 taaatcttct ataggagcct cgtccatgat aaatctgtaa cctgtcaatt caaaagcttt
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atgtagtctc cgatcaattc
    12001 tcccgtaaag gtgaaatagt tgtctaccac atcttcggta taacaggatt gaaacaaacc
    12061 cggagatatg gaaaaaagca acaaaagata aagtatcttc tttttaaagg cattggttgt
    12121 tttcatatgc tgtctatttt atttcaaagt ggatgttgta gattctgttt cataaaattt
    12181 attttgtacc agagccgtat tggcttccag ttctcctttg ttgaccggcc aatagagggc
    12241 attcatttgt gacattttac ccagggcttc gctgccttcg cctttacggg caatgaacga
    12301 aaccagaggc gcagggctgc ctgccctgcg ggccatacgc atcaggtcga accagcgctt
    12361 gccttcgaac atcaattctc gctgtctttc tctcaatacc agttcaacca gtttatcgta
    12421 agaattatcg taataatcga gcgataaagt atctcccccg ttggaacggg aataggtcat
    12481 attgaccaga gacagagcct cggccaggcc ttcttcctgt ttcccttgca gcactgcttc
    12541 tgctttcatc agaataacat cgggcaaacg atacactatc cagttcgagg tattgttgcg
    12601 ataggaataa gtgcttctgg atccgtcgaa attttcggtg cgctgaatac cggcatactt
    12661 gaatatttga taaacaccca caccgggatc gttcacaaaa aagtctttcc agcgaatatc
    12721 aacgtcgttt ttactttcta tgtagcttcc ggcagggtat tcgaaaggac tgtattgtcc
    12781 gcttaccagc gtgacaggga aagccagctg gccattctgg ttgttctggt aaccatataa
    12841 tgaacgggtg gcatcgttca cctggatatt gttatcgtaa ccgaattcaa aaatactttc
    12901 ggaagaaaat cctgtgtaaa acacctggga ataaaaattt tcggcaggta caagtgtgta
    12961 atagtcgtct gccaaaatgt aatcacacat ctgtgaacat ttctcaaatt gattatccca
    13021 caagtagata tctgccagca aggcataaac tccgtttttg gtaaaacgtc ccttggtttc
    13081 cttatccgtg gaatgtatcc gcggaatgta aggcagagaa tacagctcga gatcccgaat
    13141 gatggagtcc agtaccgctc tttcggagaa ctgcggcact ataaaatcct gagtatcgtc
    13201 cagactgggt tcactaatcc agggaacatt ctggaaagcc ctgaccaggt agaaataggc
    13261 caatgctctt acggcataag cctcagcgct gagtttacgg agcttttcca cagtaaagtt
    13321 gtcgtctttt ttaagcacat ccggagcaaa actcaaaaaa gtgttgcagt aatttatgac
    13381 ggaataaaac gacgaccagg tcgtataagg agtggaagga gtgatttcct gccggagtac
    13441 cttattgatt tcatcgccaa tgccggtacc ctgggtaaaa ttgtccgaac gtaattcccc
    13501 ccataccaac atacgatagg tgcaatccga agtaataaat ccacggtagg ctgccatcaa
    13561 cacggcttct gcttcggatt cagtggtcca gtattcatct aaaatcattt cactttccgg
    13621 acgcgtatcc agccagttgg tacaggctgt tgttgcgaaa aggctcaagg ccaatatcga
    13681 gacgataaaa agtctgtttt tcatcttttt cataatttaa aagcctatgg tcaaacccaa
    13741 agtaaagtct tttgaacgag gagtctgcgc gttatcggtg cttataccga aatcaccgta
    13801 accaatctcg gggtccatac cggaatactt agtaaacaca tataagttat taaatgtaag
    13861 ataagcgctt actctatcga tacctatggg ttttaaccaa tttgaaggaa gaatgtagtt
    13921 aaaggtcagg tatttgaaac gaaggaagga agcgtcttct acaaaacgat cgctacccag
    13981 ccagttgtat ccataattgt ataaggctct gggaatcccg gtaatatcac catccttacg
    14041 ccagcgccag ttaacagcaa tactctggtt gtcgttatcg tacatatttt cagcgttcat
    14101 gcgagcagca tttactactt tattgccgta acgatagttg aaaaaagcgg tacaagacaa
    14161 agttttatag cgtaaagtaa atcccatacc accgttaaat aggggattgc aattacccaa
    14221 atatacaatg tccaactcgt caatactacc atcgtggttc tgatctttgt atttggcatc
    14281 gccacctctg aattcatatt cgactgtagt acctgcggca aaaaccatgg gcaggggttc
    14341 acccgtttca tctgtaatta ctctgttgtc tttgtctctg gcgaccggag catccaaatg
    14401 tccgtgttct tcgtcgtatt tgtcgtattg atatacaccc tcgtactcaa atccgtagat
    14461 ggaaccgaaa gagtggcctt cctgcagacg ggtcatgtag gtgccgttgc ggaagtcgta
    14521 atctttattg tagttggcca gaatccggtc atccaactca ataatggtat ttacctgatt
    14581 ggatatattg aaattgacat ccaaactgaa gtctcctctt ctgaccaagc gattagtata
    14641 aacattgaat tcccagccgt cgttgtccat ggttccggca ttagcataag acatactgga
    14701 gaaacccgac gtagagggta gagaaatatt ggagaacaac atgtctacgg tacgtctgtg
    14761 gtagtagttc atatccatga caattttgtc cctgaagagc cctatatcgg ctccaatgtt
    14821 ccattctctc agagtggccc atttcaagtc ggacaacctc aggctgaccg gacgggttgc
    14881 ctgcttaccc aagtaactgt cgtaggcggc atagcggctg aaatgcaggt attctgcacc
    14941 gggttccctc ccgctcatac cccagttggg gcgaatggcc aacatactca gccaggagcg
    15001 ggtatcggcc ataaaatcct cgtccgaaat gatccattta agagagagac tggggaagtt
    15061 tccgaattta cgcttatttc cgaatttcgt gctgccatct ctcctgacta cacccgaaat
    15121 gatgtatctc gacttgtagg tataatgcac gcgtcctacc aaacccattg aacgccaatt
    15181 ggatcgtccg gtcgagacgc cttccagata ggcttcattg gaggcatctt tggttttatc
    15241 cgaaaataaa ccataactgg caatgtattg gctgcctgaa tttcccgtcc ttgtttgcaa
    15301 agcgccgtaa agggtcaggc tatggtcttc gtttctaagc ttaggctgcc agttgatgac
    15361 attttctgtt aataccgaaa gtgattccga gtcgttgctt tcggagcggt tcacctggtt
    15421 gctgttccag gtattgttgg tagcctccca gggtaaaaag cccgagtttt tattattatt
    15481 tatatcaaaa gagacgtaca tctgatacct aagcgaacga gaacgctccc cggtcagatt
    15541 gtaatgtaat tgaaaaatag gaacgatacg ataacttttt aggttgttta ccccgagttt
    15601 ggcaatagct accgggttta tcatgtctcg ttgagctgcg ttcaatcttg agttgcgcga
    15661 tatattgtag aatacatcgg tatcgttacc gtctctgtcc tgttcataaa tgctcacatt
    15721 gggcattttg gcataggctg tccccaggag gttagagtag ttccgctggt tatctacgtg
    15781 agtaaacgaa aagtccgaaa taaatttcag gcgttccgat acggcataat ccaaataggc
    15841 cttggaggtg aagcggttga gatgttgttc aataatcgta cccgtctgat cgtaaaaacc
    15901 tcccgaaacc ctaaaagtag cacgctctcc tcctcccgac acatttagat agtggtcgtg
    15961 agtacgaccc gtctgcgaaa cagccttgat ccagtcggta ttgttattga aattttcgta
    16021 ttctacaaaa gtgtcggaat acttaaattc atcgatatcg gcagccgatt catcctgcct
    16081 gggattaaaa taggcttgtt tgatcatcat ggaataatca tccccattga gcatttttaa
    16141 tcctttgggt tgaacggaac gggtaaaacg ataggtgtat tgtaccaaag taggcccggc
    16201 ctgacctttt ttggtcgtca ccatcaaaac tccattggcg cctttagatc cccagatagc
    16261 cgtagatgcc gcatctttca gtacagtaat ttgttctata tcgtcgggat ttatgcttaa
    16321 taactctgca tagtcctctt cgttggaagt ggcgaagtca aaatccttaa tattaacttc
    16381 gtagggcaca ccattaagca caatcagggg ctccttgtta ccggtaatcg aagcagttcc
    16441 acggatacgc atcgatgaac cgctgccggg gttacccgaa gtggaaacaa tatccagacc
    16501 agctacacgc ccctgcaaag cttcgtccag ggtacctacc tgcaaaccct caaattcttt
    16561 agcgttaatg gtttgcatgg cggtagaaat ctctctttcc ggaatcacaa atccacttcc
    16621 gtcggtgtgc atccgctggc cggttaccat agcctcggca agctctaggg cttcttcttc
    16681 catctggacg ttaatcactc tgaggctgcc aatttcaaaa gtctgtttct tgaaacccag
    16741 gtaagtaaat accaatttgt tgttggcatt tttcatcctg ataacgtatt gcccgttgta
    16801 gtcggtgata gacataccta ctacacgatt gctgacgtca agctcctgga tattaacgcc
    16861 catcaaagtt tccgaactgt tagccttatt ggtgacagtt cccttgatta tgatatcttc
    16921 ggtctgcgcc ctgagtaagc ccggaaagcc tataagaagc aaggcgatca ttgttttgac
    16981 aaatagcttc atatatatgg tttgttattg ttattaactc aattattaag tcgaactctt
    17041 tacctgtatc tcagaactct gtcgatgagg tggacaacgg ccgtagaaga agtggtgact
    17101 tcactactgc tataggctga tgtagtcaat cccgtcaagt tcttgggatt ggaattgaac
    17161 acatagtctc tggtcattat gttataatag gtccttccgt cgggtgttct cgcattgaca
    17221 tccactttgg cggtataggg tttattattc accttatcaa tatattccgt tgtcagagta
    17281 ataccggtac cgtctccttc ggcctgaata cggtagaatt tatttttgta ggtattaaag
    17341 tacgattcac tgtcgtcgtt tttgatggtt gccgtataat accttgccgg ttcaattacc
    17401 tgattgggat gaataaacag agaattatcc tgaaaatgat agcggataaa gcgctgcatg
    17461 gaatccacaa ctgcgttgta ttcggcagtg ctctctattt tggcaataga atcccaagga
    17521 ataatcagtc cggaattgaa tgcttcttcc atagcagcgt tgctggggac atataccgta
    17581 taccgatagg tgttgaaaaa agtcacatta aagtccatga aatgccggct gcctctgcct
    17641 gcttcaaaaa ttccggggaa attctcacaa taagagaaga aagcatcgaa ttcggggtat
    17701 ttagccgagt cgctcaacac cgtgtagaac gattgtaaag gagtttgagg caattgatcc
    17761 accataaaag aagtgccgtt tttttggtaa aaaatacccg aatccagcaa atgaatttgc
    17821 gttccgtttt cgatatcggc tccggcactg aagttaacca aattcgaagt cagcttactg
    17881 ccatcgatat cggctgtgcc gtcggcataa agaatattgc cgtctttggt cacataataa
    17941 ccgtcgcggt aaacggcatc atcctgatcc tccttgaaat ctccaaccac aatgctttga
    18001 tccagaattc tcgtcaagcg gttccgaata aaattagcgt tgttaagagt tgtaactgaa
    18061 tccagtacca catcgttcac tttatcgtag ttaaacacgg tcacattgac ggaagaggtt
    18121 aatctgttaa accagaattt gaggaccgta ggataattgg aactgtaggt ataaggatcg
    18181 atgtagcgtt cgagtgcctt atcggtagga atgacaaatg cgtacttact taccatcgaa
    18241 ttcaggtaca agcggtataa atatctctta ataccccagt caagcaccag ggttttttcg
    18301 ctgaatagta cgggactgta caccgacacg aagtcatcgg ggggataaat gctgctggtc
    18361 acatacacta agccgtttac ccctacataa tttaaagaat caacaatgtc cgaatttaac
    18421 acattcaaag gcgaactggc ttcgtcaacc attttatgga acagaccggg aaatgaattt
    18481 accagggact tacgcatatg cctggtaatc agcaatacta atatgtcgtt cggaacaccg
    18541 tcccaactgc cgtagcgttt ttttagctct cgtccgcttc cttcgttaaa ataataatcg
    18601 agggcctcgt tggtaggtgc aaaaataacc cccatatccg attgcagagc agctccgccg
    18661 gccgcatagg aattccagcc cggatcgtaa ggcaataatt ggtcgtcggg tatcttggaa
    18721 ccattgggat aactggtcac tcctccctga ttagtcggag caaaatacct gagataaaaa
    18781 atggaatcaa tggttgcttc gggatgcaaa ttattgtagg ttgtagtcaa gcccggcgta
    18841 tggtaggggg cacaaaaacg atcaagtaat tccgaaaact gagtaatgtt ttcgttgctc
    18901 cggatgtatt ccgccatatt gtcgcgagga atcatcactc catcgagcac attcacataa
    18961 ccgtttttac atacgatatc tcttttaatg accttatgtt gaaagatgaa agcgtcgtta
    19021 taactgcgat cggcacctgt cataacttta aaatcctggt cactgatgcc cacgttttcc
    19081 aaatactttt gcaaaaaatg aaccatcggt ttggtggaat tgtcttttaa caaatacatt
    19141 ccctttgttc tccaacgatt ccatactgtg gctttcggta atttattacc ctcaataaaa
    19201 ggcacactgt ccaatacgga caatcccgtc aaacggcgca ttgcctggcc ttcgttcaga
    19261 acaccgccgc tgtaatagtt cgaaagagtt tcaatcaaat acgcattatc gatcatcgag
    19321 aaaaacaaca attgtcgttt ctgggacaga ttcatttcat cgaaactatt caatccccat
    19381 acattgtcct gaaaaaagcg gtcaaaaata ctgtcggggg taacaaacag ggttttactg
    19441 ccggttttgg ccaatacttc tttctgaccg acatcctcga ttattttgac ataataatca
    19501 aaatgaccgt cctgtttcag gtaatcatag atgctttcgc ccaaccagtc ggggcttttt
    19561 tcatcgtaga tataatcgtc cttgcacgat acaagaaaca ctaatccgaa tacgacactt
    19621 aatcgcatca gtagtcgaaa gggattttta aattctttca tcagattcat atgggtttgg
    19681 atttatttag aaagtcaact gcaacgaatt tcatattgtg aatttcataa gtttttcaaa
    19741 aatttagtca caaatatatg ccataacatc agcatcaggg tggatgattc attcaaataa
    19801 gtggacaaga aaataaaatc aagtttttta acaagcattt tgtccaccaa ttataaacat
    19861 ttatccacca aaaatataca caatacgcat atttttgcga agtgtgttta caaaatcggg
    19921 aggtggagaa gataaaaaac aaaaaatgaa aataaattcc tgtccactct aattattgta
    19981 ctgcccgcaa gtgttatttt actttagtat attgtcttaa tttttcttat ttcgcaaatt
    20041 ctttttacct ttcaacacca ctaactttaa actttatgaa aatgaaaaaa aactacctca
    20101 aattggcaac caggacttgc ctgcttgcca ttacttgtat gcttgcaact tcagcattgg
    20161 ctgatattac aagcaacctg catttgcatt atactttcga tgggcttact gcggagcagt
    20221 ccgaagtacc cgatgtttcc ggcaataaca acgcaggaac acgcatggga ggcgcctatt
    20281 atgtcgaagg taaattcggt gatgctctcc gtcttgaaac cgtgaacgac tatgtatata
    20341 tgcccgacgg ctttatggtg gggatggagg acataaccat tgccggctgg atatacaaag
    20401 atgccgccaa tggttgggac cgcttggtag actttgctac caacaccgac accaacatgt
    20461 ttattaccac aggaggaccg ggttacgtgc gttttgccat caaaaacatt caggttcgtc
    20521 ctgcagggga agaacacatc aatggcaagg ctctcacctt tggccgctgg aatcacattg
    20581 cggttactat caaatacacc aacaacatcg gcttgggtat tttgtatgta aacggtatcg
    20641 aagtaggtcg taacaacaac ataacactga agcccagcga tcttggattt acgacgaaaa
    20701 actacgttgg gaagtcggtt tacactgctg acccctcggt ggtgggaaac atagacgatt
    20761 tgcgcattta caaacgtgcc ctcaccgccg acgacattgc cgagttactt ggcgtacccg
    20821 acgaaattac ggattgttac aacaatctag cattggatct tcctgaagat tacgaattta
    20881 ccggtttgac cgcagatttg acgctgccta ccacctgggc cgacagcgct gtaagcatca
    20941 gctggacatc ttccgacacc ttgctgatcg acaacgaagg caaaattgtg gctttccccg
    21001 aaaaatacca ggatgcctgt atattgacag cctccttgtt gcagatgaga ggcgaggaag
    21061 aatttaccgc cgaacgcacg ttcatcgtaa aggtttatcc ccaggaaaca gaagtcaatc
    21121 ccatcattgc cgtttgggac tttacggaag aaaccctggg taccgatgaa gacggaaatg
    21181 taaccgtaac ggataaaagt ggaaacgatt ttgtgggaac ctgtctgggt ggagcccgta
    21241 tcgtcaccat gggcgaaagc aatccggtca gcgccatgta tattgaacaa gccggtgctt
    21301 actttgatat gggccaagaa ataggtaaag ccgtggccgg attaaccgac tacaccatca
    21361 gtgtgtttta ccgcaaagac agcgtaggtg gcgttacctg gagcagcagc gcctacggtc
    21421 aatggttgta ttgcttctgc aacagcaacg accttgccag cgatggacag gggggtatat
    21481 tctacgaacc ctatcgcgcc taccacgccg tattcaccac caactacggc gacgagactg
    21541 gcgttcaaag aggacaaagg agaactccta taggtacctg gcataatatt acttacgtac
    21601 aaagcggcca aaaaggcact ctgtattttg acggcgaggt ggccggaagc agcgacaacg
    21661 tttatattcc tgcctatgct ctcaaagcaa agggacacag aggcaccatt cacaattaca
    21721 tcggccttcc ctgttattcc ggcgacccaa aattgtccaa aactcatatt gccggcttgc
    21781 agatatacgg agttgctctt accc
//
LOCUS       DAOSTD010000025        21474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_2649_length_21474_cov_16.144731, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000025 DAOSTD010000000
VERSION     DAOSTD010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21474
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2649_length_21474_cov_16.144731"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="PLI37_02900"
     CDS             complement(<1..1373)
                     /locus_tag="PLI37_02900"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOQ58001.1"
                     /translation="MRSKHFILRYRWWIIAAVVLTVALCIWPLTRTTVNPDLESYLPD
                     SMQSKQNSRKIEEIFGAEEPMLIVLESDDILNKSSLERIEQLDRAFSMQPAFKRVFSL
                     FGVQDIHSEEGVMLVDPLIDAIPQSPAETDILRERIKSNDLAYGLVVSDDFRYSLILL
                     SSDKSLEDAELLNIIKQTLEKYPGDEKVMISGQPLLREEASIKIGRDLMILLPLGLLV
                     MFVFLWVSFREGKGVFLPISVVIFSVVVSMALIPLFGWELSIIGVLIPIMMLAIANNY
                     GVYFIARYQDLNAQDPRLTMPQIVQKSFSYLFKPVLFCGLTTMVGIMGLTTHLLIPAR
                     QMGVVTGLGIAFALVASLLFVPAVLSLMKKGKPHHDLTGKPNGPISAFLYRAAVAVVR
                     HPKRVLIGFSVFFALAVTGLLFFKLSPDTNNVLPEKHTFNRAIKAVDEHMGGSKMITL
                     MFEADAK"
     gene            complement(1392..2735)
                     /locus_tag="PLI37_02905"
     CDS             complement(1392..2735)
                     /locus_tag="PLI37_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58002.1"
                     /translation="MKRLILSFFLFTATVGLASAQGLFETAGKSPDNQSEKTVKLGGY
                     VRGSAYGAGENYDFGTLFGETALQLNHKAKLLTMNTDLRFRSGNRFGGSFSEFEIKEA
                     YAGISSKTVDVLLGEQIVSWGRTDGFNPTNNINPNNYFFLSGNPDDQKMSNFMLRSKV
                     RITPMIDWEAIVIPIYRPSVYRYELFDLGASFVDPVLPEQKAGNGSLATRFNFELPGV
                     GFSLSWFQGRDPFYGINLNSVDLSSGTPQVSYRPAFYRRTTFGLDFAVPLSSWIIRGE
                     GAYNFTENEHARMYIPNCDLSYVMGVEYNFGSVTAILQYVGKHVLDFVDLNEPVLSDP
                     MNQLALYQYANERVNYESFLINRKIFKQQFETNHALALTLSGDFVYSTLNAEMTGFYD
                     FSTEEFLLRPKLVWNITDALALSAGYSYMKGPDKSMFSYAGPVLNGAFLEMKFSF"
     gene            complement(2864..3973)
                     /locus_tag="PLI37_02910"
     CDS             complement(2864..3973)
                     /locus_tag="PLI37_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ58003.1"
                     /translation="MAKIASMDVIVWIGFAQALFAGILTITKDKKSSADYLLSAWFFL
                     LSIEFLTVGIELYVYTNFVFLSNTFLLFNPALFLYTRSLVKPSFRLKWPQLLHLLPYL
                     IFKIFSWSLQEPLSFEGFFSPDNTLWYRISFGVFGLVSWIVYLGLTAVILSRHRKRLK
                     NEFSTIDRIKKMGWIMFVVVFYGIYCLIVLLWGLFNIIVFTTESLTLFNYSVLLFFVY
                     IFSFYGLKQRDIFPDKTKEEPVVPEKNSNSFLSTDQMEKISQKLLNYFQKEKPYLNPE
                     FSMSLLSESLKIPRHHLTEVLNKHLGKNFFLFVNEYRVAAVKKQLDKPDNPYSIEAIG
                     YECGFNSKSTFFSVFKTLTGQTPKNYQQEVLQKKN"
     gene            4122..5501
                     /locus_tag="PLI37_02915"
     CDS             4122..5501
                     /locus_tag="PLI37_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOQ58004.1"
                     /translation="MKNYSLVALLLLTVFGIQAQLPVRNAENIEKYKDICRTYIHKEM
                     KGMYREAGGALPYPFLAPGSNQYLDMLWDWDSWLSNVALRQILLEKGKEKDREEALKY
                     EQGCVLNALSYGGMDGWIPIWIERDAPSRQEMLSTRNPWKSNMHKPCLAQHAAFIVRN
                     INGDAEWLRESFYYLQAFVNKYLNFHRHQATGLMYWQTDEAIGVDNDPSTFYRPDASS
                     GSIYLNCLMYKELLAMDYLAQCLNLKDIAAFYNKEAARLLENIREHCWDPRDGFYYSV
                     DLNLRPIEKPDIEFLEAGKLYLHTGQPRTYHCLIQRFSVWSGFMAMWSGVATDEQAAL
                     MVERHFHDTTSFNSASGIRSLSPLEKMYDTRGSGNPSSWQGPIWINVNYLVFRGLINY
                     GYKEEARELAEKTILLLGRDIERFGAMHEYYLPDNGEPVLNRGFQNWNFLVLNMAAWL
                     DGKDVVWEF"
     gene            5513..6874
                     /locus_tag="PLI37_02920"
     CDS             5513..6874
                     /locus_tag="PLI37_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HOQ58005.1"
                     /translation="MSRFIYIITILCLLPVTDLRAGNNREKPRVIVTTDGEIDDQSSM
                     IRFLMHASDYDVAGIVQVNGVQKDGHSKDRWIEAQIEKYAEVLPNLRLHHPDYPDADY
                     LLSVLKVGNETRDDMYKAPPLLAHTEGAQLIIDALLDDDPRPVHILAWGGANTQANAL
                     WQIKQHYSTEQWKRAAKKARLYCIWYQDGGGKWIEENLPEILIYESGAPFRDGGWRYV
                     WDYMSVDYYFKNRISKNPPELQKLMDKPWLAEHITSGHGPLGAAYPQDYTSEGDTPSY
                     MPLIDNGLEQHTDYRLGGWGGRPITVKGNHLQDGDDLDPSVDSLSSHYAFQRWLPAAQ
                     NEWAARADWCVKSYADANHPPVLTLTTPARIEAQPGQKIKLQINATDPDGDQLSYRWW
                     PYREAGTYEGKLSIKQADRKKASLTIPLDAKPGQTIHLICEVTDNGTPPLVRYRRVVV
                     RVR"
     gene            complement(7016..7099)
                     /locus_tag="PLI37_02925"
     tRNA            complement(7016..7099)
                     /locus_tag="PLI37_02925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7064..7066),aa:Leu,seq:taa)
     assembly_gap    7169..7268
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7269..7533)
                     /locus_tag="PLI37_02930"
     CDS             complement(<7269..7533)
                     /locus_tag="PLI37_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58006.1"
                     /translation="MKKIISLLLFLGFFACGRYQSSIPDSPVFMRRNLNTIKCLFPGD
                     FYYITEPETVTDRLGYGGLVLLKGFDDRYYAYDLACPVECRPDV"
     gene            7691..9778
                     /locus_tag="PLI37_02935"
     CDS             7691..9778
                     /locus_tag="PLI37_02935"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOQ58007.1"
                     /translation="MKKSVLILCTLMMISCAKKQGNPFFEDYDTPYGAVPFDKIGIEH
                     YMPAFVEGMNRQKLEIDAIAGNPEAPTFENTIAAMDYSGELLNKVSSVFFGLISSHTS
                     DEMQAIAQELSPLLSEHSDNIRLNAPLFKRIESLYNNRDSLNLNTEQQRLLEKYYKNF
                     VRSGAALDESRQERLREINKEEALLTLQYSDNVLKETNDYRLVIDKEEDLSGLPQTVV
                     EAAAEAAAGAGLEGKWVFTLHNPSRLPFLQYADNRELREKILKAYCMRGDNNNEADNK
                     EIVRKLVNLRVEKAQLLGYNTFAEYVLEETMAKNPKNVYDFLAGIWPRALRQAKKEVT
                     ELQKLIDREGGKFKLQAWDWWYYAEKLRKEKYQLDEEELKPYFELQNVRQGLFTVCNK
                     LFGINLTPVNNVPVYHPDVEVFEVTDADGSHLGLFYSDYFPRESKRGGAWMSNFREQY
                     VKNEQNVRPHIYNVGNFTKPSGNTPSLLSLDEVETMFHEFGHTLHGMLSQCSYPSLNG
                     TNVTRDFVELPSQIMENWCTHPEVLKMYARHYKTGEPMPEELIEKIQRAATFNQGFIT
                     TELMSAAYLDMYWHDLSEQKDYDVNQFETDCLSRIGLIPEIVVRYRSTYFNHIFNNNY
                     HAGYYSYLWAEVLDADAFQAFVENGLFDQKTATAFRQNILERGGTDDPMTLYRKFRGA
                     DPDPKALLERRGI"
     gene            9902..>12876
                     /gene="secDF"
                     /locus_tag="PLI37_02940"
     CDS             9902..>12876
                     /gene="secDF"
                     /locus_tag="PLI37_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOQ58008.1"
                     /translation="MQNKGFVTLFAVLLALVSLYYLSFTAAVKNTEKKAKEYAQGDPV
                     LERNFLDSMAQEKIYLGNTYRECLEKEIGLGLDLKGGMNVVLELSVPDVLRALSGYNT
                     DENFNKALAQASLRMRESQRDYLDLFVEEYKAIDPGARLSAIFSTFEMKGRIDPSSSD
                     ERVIQVLREDIKSAVDNSFNVLRTRIDQFGVVQPNIQRLETGGRILVELPGIKEPERV
                     RKLLQGSANLEFWETYELSELYQALIEANNAVRDMIAATEPQKEQVAEAAPVEKAPVE
                     QVSDTAKTEEERLLEQLAAETEIQTDEQSLEEWKREYPLFAVLQINQSQQGIGAGPCV
                     GYALGRDTAKVNEYLNSRNVRELLPRDVKFLWSVKPINEKTPLFELIAIKVTNRDGRA
                     ALEGDVITDARDDFEQMSGSAVVSMSMNAEGSKTWARITKDNIGKCVAIVLDNYVYSY
                     PRVNTEITGGSSQITGNFTSAEAKDLANVLKSGKMPAPARIVQEDIVGPSLGKEAIKS
                     GLISFAIAFAIVLIYMIFFYGIIPGLVADFALLCNIFFLMGILASFQAVLTLPGIAGI
                     VLTLGMAIDANVLINERIKEELAAGKSIKKALPDGYKNAFSAIFDSNLTTLLTGIILF
                     ILGTGPIKGFATTLSIGILTSFFSGVFITRIIFERLLAKEKLNNITFTTKLSKNILRN
                     PQVNFLGLRKKGYIVAGILVLACILSWSTRGLSQGIDFSGGRNYVVRFDQPVSTQDVR
                     NSLDGIFEDASLSVITIGSENQVRVSTNYKINETYDELDAEIDSILFTGLKDYLPENT
                     GLESFVEDYIVSSQKVGPTVASDIKISAIWSVVAALLVIGLYILLRFRNVAFSVGVVA
                     SLAFVTIFIIGLYSMLYSVMPFSLEIDQTFIAAILTVIGYAVNDSVVIFDRIRETTGL
                     YPKRHKRLTMNSAMNTTLLRTFSTAFSTALVLLAILLFGGDTIRGFAFAMLIGVVFGT
                     LGTLFIASPVAYDIQRKRE"
     assembly_gap    12877..12976
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12977..13258)
                     /locus_tag="PLI37_02945"
     CDS             complement(<12977..13258)
                     /locus_tag="PLI37_02945"
                     /inference="COORDINATES: protein motif:HMM:NF023074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3256 family protein"
                     /protein_id="HOQ58009.1"
                     /translation="MRKVVLLLLITCFIVPGVWSRSVVECFKDMPADLLPGLNTAMKL
                     DMVDFFNSGKTARMLGLLGSEMELKILTEDYLMLQTSAASNVQLKLLPLK"
     gene            complement(13272..14174)
                     /locus_tag="PLI37_02950"
     CDS             complement(13272..14174)
                     /locus_tag="PLI37_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOQ58010.1"
                     /translation="MWLWATFASSLLLGVYEVYKKLSLKENAVVPVLFLTTVFCSLFF
                     LPLLLLSEWAPQSLENTLFFVPRADAKTHLFILLKSFIVLSSWAAAYYAIKNLPLTIV
                     SPIKSTQPVFVLMGAFMVFGERLNLWQWLGVLVSIISIFLYSYVGKKEGISFRRNPWI
                     WCLFLSVLIGSVSGLYDKYMMVNFDRMTVQVWYTIYQIFIMLPLLLIYMHRRKRGKEQ
                     AFDFRWSIVGLAVFLCLADFLYFYALSRPGALISVISPIRRSGVLIPFVVGALFFKDK
                     NIKAKAWILLGVFIGMCCLYLGSK"
     gene            complement(14185..14940)
                     /gene="truA"
                     /locus_tag="PLI37_02955"
     CDS             complement(14185..14940)
                     /gene="truA"
                     /locus_tag="PLI37_02955"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOQ58011.1"
                     /translation="MQRFFLNFSYDGSAYHGWQIQPNAISVQEVMTSVLRTLFEKDLD
                     IIAAGRTDSGVHALAMTAHFDTAYPIENPDAMANKLDRMLPADIAVRSLRRVRPGAHA
                     RFDAISRTYQYKIILQKDPFLRDYSLRLYYEPDVEAMNKAADRLLEYSDFTSFSKVHT
                     DVKTFICKVTHARWERRGSELIFTIQADRFLRNMVRAIVGSLLEVGKGRIDEEGFCRI
                     IEARDRCRAGTSMPAHALYLTEVKYSDDLYLIG"
     gene            15036..16022
                     /locus_tag="PLI37_02960"
     CDS             15036..16022
                     /locus_tag="PLI37_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOQ58012.1"
                     /translation="MNEKILFLEQVDPSVFCGVNNSNIKLLKDLYPKLRIIARGNVIK
                     ILGEEAEIARFEQLINHLQDYLNKYNLINQDVITQLNQGKPLFEPKQENLILYGVNGK
                     PILARTINQQKLVKAYEKNDLLFAVGPAGTGKTYTAIALAVRAFKNKEVRKIILSRPA
                     VEAGEKLGFLPGDMKEKVDPYLQPLYDALEDMIPPFKLREYMENKQIQIAPLAFMRGR
                     TLNDAVIILDEAQNTTGLQIKMFLTRLGLNAKMIVTGDLTQIDLPAATTSGLVESLKV
                     LHGIEGIGKVEFDVRDIVRHKLVKHIVKAYEDYQQEQKKLKNTRTDRNNPNE"
     gene            16034..16984
                     /locus_tag="PLI37_02965"
     CDS             16034..16984
                     /locus_tag="PLI37_02965"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOQ58013.1"
                     /translation="MKQALVKTDFNFDGQISVYHGKVRDVYNIEDELLVMVVSDRISA
                     FDVVLPKGIPYKGQVLNQIAAKFLDATSDIVKNWKIAVPDPMVTVGVKCEPYEVEMVI
                     RGYLTGHAWREYKSGKRSLCGIQLPEGMKENERFPEPIITPTTKAKEGHDEDISREEI
                     IASGLVGKAEYEKLEEYTRAIFKRGTEMAAQMGLILVDTKYEFGKKDGEIILMDEIHT
                     PDSSRYFYADGYEAKLAANLPQKQLSKEFVRQWLIENGFQGKSGQQVPYMSDEYCRSV
                     SERYIELYEKVTGENFVRAETEDVKARIERNVTQFLKQYR"
     gene            16987..18156
                     /locus_tag="PLI37_02970"
     CDS             16987..18156
                     /locus_tag="PLI37_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOQ58014.1"
                     /translation="MSDQRYQLRGVSAGKEDVHRAIKNIDKGLYPKAFCKIIPDILGG
                     DPAYCNIMHADGAGTKSSLAYLYWKETGDLSVWKGIAQDALIMNIDDLLCVGACDHIL
                     VSSTIGRNKHLIPGEVISAIINGTEELLEELRSMGLGVYSTGGETADVGDLVRTIIVD
                     STVSCRMKRADVIDNANIKGGDVIVGLASFGQAVYEKSYNSGMGSNGLTSARHDVFAH
                     YLAQQYPESYDPNTPEKLIYSGQLRLTEPVPGTGMTAGQLVLSPTRTYAPVIKAVLNE
                     LRPRIHGMVHCTGGAQTKVLHFVENKRVVKDKLFPLPPLFKLIQEQSGSDWKEMYKVF
                     NMGHRMEIYLDPSDAERVIEIAAGYGVEAQIIGRIEEARANQLIIESEYGRFEYY"
     gene            18243..19328
                     /gene="prfA"
                     /locus_tag="PLI37_02975"
     CDS             18243..19328
                     /gene="prfA"
                     /locus_tag="PLI37_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOQ58015.1"
                     /translation="MTEHSLLDRLDWLQSRFEEVSTLIASPDAVSDMKRYVRLNKEYR
                     DLEQIVLARREYLQLINNINEAKALLEHESDAELREMAREELNKETARQPQLEEKIKL
                     MLIPADPQDSKNAIVEIRGGTGGDEAAIFAGDLFRMYSKYCESKNWKVEVTNSNPGTS
                     GGYKEIIFTVSGEQVYGTLKYESGVHRVQRVPLTETQGRVHTSAATVAVLPEAEEFDV
                     ELNESDIKVDTFCSSGPGGQSVNTTYSAVRLLHIPTGVVVQCQDERSQLKNKAKAMNE
                     LRSRLYDMEYQKYLEEISSKRKTMVSTGDRSAKIRTYNYPQARITDHRINLTMYNLSA
                     VLNGELQEIIDQLIIAENAERLKAGGY"
     gene            19341..20168
                     /gene="pyrF"
                     /locus_tag="PLI37_02980"
     CDS             19341..20168
                     /gene="pyrF"
                     /locus_tag="PLI37_02980"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOQ58016.1"
                     /translation="MNRQEIISQIKTKATFLCIGLDPDIQKIPSHLLKGEEPLFAFNK
                     AIIDATHDLCVAYKPNLAFYESCGLEGWKAFEKTVRYIREKDPTQFIIADAKRGDIGN
                     TAEMYARSFYESLPIDAVTLAPYMGKDSVQPFLSYPGKWAILLALTSNRGSADFQMLE
                     DAQGERLFEKVLRRSSDWGDEFSMMYVVGATQGSLLQDVRKLVPRHFLLVPGVGTQGG
                     SLKEVARYAMNDDCGLLVNASRQILYASKEEEFAQAARQEAMKLQQEMQTYLKALWG"
     gene            20159..21379
                     /locus_tag="PLI37_02985"
     CDS             20159..21379
                     /locus_tag="PLI37_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ58017.1"
                     /translation="MGMKQKIINDPVFGFINLPTGFLYQVYQHPYLYRLSRIQQLGLS
                     FLVYPGARHCRFQHITGALHLASEAIRQLRLKGNEITEAEAEGTVAAIMLHDIGHGPF
                     SHVLEPVLFNGLSHEKISLLLMERMNKEFNGKLDMAIAIFKGSYPKKFLHQLISSQLD
                     VDRLDYLRRDSFFTGVVEGNIGSSRIIKMLAVCEDRLVVEAKGIYSIENFLMARRLMY
                     WQVYLHKTSLAAEKMLHNLLHRAKVLKQAGQDLTCSPALSFFLSGKLKPGEISDVALD
                     HFIALDDTDIWSAIKNWQNHPDVVLSTLSRNFLNRKLFKIEISEQETPPSRVQEDLQR
                     IALQLNIGIEEARYFISLNKVSSHIYDDADYGIDILYNDGRIRPITQASDILNLEVLS
                     KKVRKYAYSYLRKV"
BASE COUNT         6112 a         5314 c         4876 g         4972 t
ORIGIN      
        1 tctttggcat cggcctcaaa catgagcgta atcattttac tgcctcccat atgctcgtca
       61 acagctttaa tggctctgtt gaaagtgtgt ttttcgggca gcacattatt ggtatcgggg
      121 ctgagtttaa aaaacagcaa accggtgacg gccagcgcaa agaacaccga aaaaccaata
      181 aggactctct tagggtgacg aaccactgca acggccgccc ggtacaaaaa agcagaaatg
      241 ggcccattgg gtttaccggt taaatcatgg tgcggtttac ctttcttcat caacgacaac
      301 acagccggaa cgaacagtaa actggctacc aaagcaaagg ctattcccaa gcctgtaacc
      361 acacccattt gcctggccgg aatcaataaa tgcgttgtca aacccataat tcccaccatg
      421 gtggtcaatc cgcaaaacag taccggctta aacagatagg aaaaagattt ttgtactatc
      481 tgaggcatgg tcaggcgtgg atcctgagca tttaaatcct gataccgggc aataaaatag
      541 actccgtaat tattagctat agccagcatc attataggaa tgagtacacc gattatactc
      601 aattcccagc cgaaaagggg aatcagcgcc atgcttacca cgacggaaaa aatcaccacc
      661 gaaatcggca aaaacacccc tttgccctct cgaaaagaca cccacaaaaa cacgaacatc
      721 acaagcaaac ccaggggaag taaaatcatc aggtcccgcc caatcttgat actggcttct
      781 tcgcgcaaca aaggctgacc ggaaatcatc accttttcgt cgcccgggta tttttcgagg
      841 gtttgtttga tgatattcaa caattctgcg tcttcgagcg atttgtcgga gctcagcaaa
      901 atcagactgt aacggaaatc gtccgaaacc accagtccgt aagccaaatc gttgcttttt
      961 atcctctctc tgagaatgtc cgtttcggca gggctttgag gaatggcatc tatcagcgga
     1021 tctacgagca tcacgccctc ttccgagtga atatcctgaa ccccgaacaa agagaaaacc
     1081 cttttaaagg caggctgcat cgaaaaagcc ctgtcgagct gctctatgcg ctccaggctg
     1141 cttttattta aaatatcgtc gctctctaat actatcagca tcggctcttc cgccccaaaa
     1201 atttcttcta tcttccggct gttttgtttg gattgcatcg agtccggcaa atagctttcc
     1261 agatcgggat tgacagttgt cctggtcaat ggccaaatac ataacgctac cgttaataca
     1321 accgcagcta taatccacca gcggtagcgt aaaataaaat gtttagatct catcgtcgtc
     1381 tatcttgata atcaaaaact gaacttcatc tccagaaaag ctccgttcaa aacaggacct
     1441 gcatagctga acattgattt atcggggcct ttcatatacg aatagccggc actgagtgcc
     1501 agggcatcgg taatattcca gaccaatttg ggtctgagca ggaattcttc ggtagaaaaa
     1561 tcgtaaaagc ccgtcatttc agcattcaga gtgctataga caaaatctcc cgacaaagtc
     1621 aaagccaggg catgattcgt ttcgaactgt tgtttaaata ttttgcgatt tataagaaaa
     1681 gattcgtaat tgaccctctc attggcgtac tgatacaggg caagctgatt catagggtcg
     1741 ctcagcaccg gttcgttcaa atcgacaaaa tcgagaacgt gtttacccac gtattgcaaa
     1801 atggccgtaa cggaaccgaa attatactcc acccccatca catagctcag gtcacaattg
     1861 ggaatataca ttcttgcatg ctcgttttcg gtaaaattat aggctccttc tcccctgatt
     1921 atccaggaag acaaaggcac ggcaaaatcc aacccaaagg tggttctgcg ataaaaagcc
     1981 ggtctgtagc tgacttgcgg agtacctgag ctcaaatcta cgctgttcag gttaatgccg
     2041 taaaagggat cgcggccctg aaaccaggag agtgaaaaac cgaccccggg caattcaaaa
     2101 ttgaagcggg tggccaggct gccgttgccc gctttttgct cgggcaacac cggatcgaca
     2161 aaagaagctc ccaaatcaaa taattcgtaa cggtacaccg acggccggta aatgggaata
     2221 acaatggcct cccaatctat catgggggtg attcttacct tgcttctgag cataaaattg
     2281 gacatcttct ggtcgtccgg attacccgac aagaagaagt aattgttggg attgatattg
     2341 ttggtaggat tgaatccatc ggttcgcccc cagcttacaa tctgctctcc cagcaggaca
     2401 tcaaccgttt tagaactgat gccggcgtaa gcctctttga tttcaaactc cgaaaaagag
     2461 ccgccaaaac ggttgccgct acgaaaacgc agatcggtat tcatcgttaa aagcttggct
     2521 ttgtgattga gttgcaaggc tgtttcgcca aataaagtac caaagtcgta gttttcgccg
     2581 gcgccgtagg ccgaaccgcg aacgtaaccg cccaatttga cggttttttc gctctgatta
     2641 tccggcgatt tacccgcggt ttcaaacaaa ccctgtgcag acgcaaggcc gactgtggca
     2701 gtaaatagaa agaatgataa aataagacgt ttcataaaag cgcattttaa ataattaatg
     2761 ttgcgccaaa atacagtttg cactgcttcc tacggtctta ttttaaaaag tcggagaagt
     2821 tgaagaagta cgattttaaa aagatggact aaacggattt cggttaattc tttttctgta
     2881 aaacctcctg ttgataattt ttgggagttt ggccggtaag ggttttgaaa accgagaaaa
     2941 aagtagattt gctgttgaag ccgcattcgt aaccgatggc ttcgatcgaa tagggattgt
     3001 cgggtttatc cagttgtttt ttaacggccg ctacccggta ttcgttcaca aagagaaaaa
     3061 agtttttgcc caggtgtttg ttcagcactt ccgtgagatg atgccgcggt atttttaagc
     3121 tctccgacaa aagactcatg ctgaattccg ggtttaaata gggtttttct ttttgaaaat
     3181 agttcaacaa cttctgactt atcttttcca tttgatcggt actcaaaaaa gaattggagt
     3241 tcttttcggg aacgaccggt tcttcttttg ttttatcggg aaaaatatcg cgttgtttga
     3301 gcccgtaaaa actgaatatg taaacaaaaa acaagaggac cgaataattg aaaagcgtca
     3361 gcgattcggt ggtaaatact ataatattaa acaaacccca caacagcact atcaggcaat
     3421 aaatgccgta aaaaacgacc acaaacatga tccagcccat ttttttaatg cggtcgatgg
     3481 tggaaaattc gtttttaagc cttttgcggt gacgcgataa aatgacagca gtgagcccca
     3541 aatagactat ccaggaaacc aggccgaaaa caccaaacga tatccggtac cacagcgtat
     3601 tgtccgggct gaaaaatccc tcaaacgaca aaggttcctg cagactccac gaaaatattt
     3661 taaaaattaa ataaggcaat aaatgcagca attgcggcca tttgagacga aacgagggct
     3721 ttaccagaga acgggtataa aggaacaaag ccggattgaa cagcaaaaaa gtgttggata
     3781 aaaacacaaa attggtgtaa acatacaact ctatcccgac cgtaagaaat tctatactca
     3841 ataaaaaaaa ccaggccgaa agcaggtaat cggctgaact ttttttgtct ttagttatgg
     3901 taagaatccc tgcaaaaagg gcctgggcaa aacctatcca gactataaca tccatgcttg
     3961 caattttcgc caaaggtaca gaaattaatc tttgagcgca cagaaaaaaa acctactttt
     4021 gggacatctt atttcaacat cacggcttta aaggcttgaa aaaccggtgg aaccaatttg
     4081 ttttcaccgg cttgattcgc caagcgtcat ttataaacat tatgaaaaat tattctttag
     4141 tcgcattgct cctgctcacc gtattcggca tacaggccca actccccgta cgaaacgccg
     4201 aaaacatcga aaaatacaaa gacatctgcc gaacctacat tcacaaagaa atgaaaggta
     4261 tgtaccgtga agccggcgga gctctgccgt atcccttttt agcgcccggc agcaaccaat
     4321 acctcgatat gttgtgggat tgggattcct ggctgagcaa cgtagctctg cggcaaattc
     4381 tactggaaaa gggcaaggaa aaagaccgcg aagaagctct taaatacgaa caaggctgtg
     4441 tgctgaacgc cctgagttac ggcggtatgg acggatggat tcccatctgg atagaacgcg
     4501 atgccccctc aaggcaagag atgctgagca cgcgcaatcc ctggaaaagc aatatgcaca
     4561 agccctgcct ggctcaacac gccgccttta ttgtgcgcaa catcaacggc gacgccgaat
     4621 ggctgcgcga atcgttttat tacctccagg cctttgtaaa taaatacctc aattttcaca
     4681 ggcatcaggc caccgggctg atgtattggc aaaccgacga ggccataggc gtggacaacg
     4741 atccttccac tttttaccgt cccgatgcaa gctccggctc catctatctg aactgcctga
     4801 tgtacaagga actgctggct atggactact tagctcaatg cctgaactta aaagatatcg
     4861 cggcttttta caacaaagaa gccgcccggt tgctcgaaaa catccgggag cattgctggg
     4921 atccgcggga cgggttttat tacagcgtcg atttgaacct gcgccccata gaaaaaccgg
     4981 acattgagtt tttggaagcc ggcaaactct acctgcatac cggccagccc cgcacctatc
     5041 attgcctgat tcagcgcttc agcgtttgga gcggatttat ggccatgtgg tcgggagttg
     5101 ccactgatga acaggcagcc ctgatggtgg aacggcactt ccacgatacc acttcgttca
     5161 actcggcctc cggcatccgg agcttatcgc ctctcgaaaa aatgtacgac acccgtggca
     5221 gcggcaaccc ctcatcctgg cagggcccga tatggatcaa cgtcaattac ctggtgttcc
     5281 gcgggctgat caactacggc tacaaagagg aagcacggga attggccgaa aaaaccatcc
     5341 tgttgctggg cagggacatc gaacgcttcg gagccatgca cgaatactac ctgcccgaca
     5401 acggagaacc ggtgctgaac cggggctttc aaaactggaa tttcttagtc ttgaacatgg
     5461 ccgcctggct cgatgggaaa gacgttgttt gggagtttta aaatataact gtatgagtcg
     5521 cttcatttac attatcacca tactttgtct tctgcccgtc accgacctgc gggcaggaaa
     5581 caacagagaa aaaccgcgcg tgatagtcac caccgacggc gaaatcgacg accagagttc
     5641 aatgatacgt ttcctgatgc acgcctccga ctacgacgta gcgggtatcg tacaagtcaa
     5701 cggggttcag aaagacggac acagcaagga tcgatggatc gaggcgcaaa tcgaaaaata
     5761 cgccgaagta ttgcccaatc tgcggcttca ccatcccgat tatcccgatg cagactatct
     5821 tttgagcgtg ctcaaagtgg gcaacgagac ccgcgacgat atgtataaag ccccgccgct
     5881 gctcgcccac accgagggcg cacagttgat catcgacgcg ttgctcgacg acgatccgcg
     5941 tcccgtgcat attttggcct ggggcggcgc aaacactcaa gccaacgcat tgtggcaaat
     6001 caagcaacat tattctaccg aacagtggaa acgggcagcg aaaaaagcgc gtctctattg
     6061 catctggtat caggacggcg gaggaaaatg gatcgaagag aacctaccgg agatactgat
     6121 ctacgaatcg ggcgccccgt ttcgcgacgg cggttggcgc tatgtctggg actacatgag
     6181 tgtggattac tatttcaaaa accgtatcag caagaatccc ccggagttgc agaagttgat
     6241 ggacaagccc tggctcgccg aacacatcac ctccgggcat ggccccctgg gagcagccta
     6301 cccgcaagat tataccagcg agggcgatac tccttcgtac atgccgctga tcgacaacgg
     6361 actggagcaa cacaccgact acagacttgg cggctggggc ggacgcccca taaccgttaa
     6421 aggcaatcac ctgcaagacg gcgacgacct cgacccctcc gtcgacagtc tcagttcgca
     6481 ctacgccttc caacgctggc tccccgccgc ccaaaacgaa tgggcagcac gtgcagactg
     6541 gtgcgtaaaa tcctacgccg acgccaatca tcccccggtg ctaacattaa ccacacctgc
     6601 ccgcatagaa gcccaacccg gccaaaagat caagcttcaa atcaacgcca cagaccccga
     6661 cggcgaccaa ctaagctacc gttggtggcc ataccgcgaa gcaggcacct acgaaggcaa
     6721 gctaagtata aaacaagccg accggaaaaa agcctcgcta accatccccc tcgacgccaa
     6781 acccggccaa accatccacc taatctgcga agtaacagac aacggaacac cacccctagt
     6841 ccgctaccgg cgggtggttg tgagggttcg ttgagtattg ttgtttttat ctaagggatt
     6901 atacttgctg accgaagaag agatacaaat cctgcccata ttgtatcttg gagaaaaatc
     6961 atcaagaaga aacacagaag ccccacccaa tcgatcgatt ttgtggggct tccggtgccc
     7021 gaaaccggac tcgaaccggt acagcctcaa tggccaaagg attttaagtc cttcgtgtct
     7081 accattccac cattcgggct gccttttata gaacaggaac gcattaatgg ctttacatta
     7141 agttgcttgg catattgaat agcttcgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnta acgtcgggac ggcattctac cgggcaggcc aggtcgtagg catagtaccg
     7321 gtcgtcgaaa cctttgagca acaccagacc tccgtaaccc aggcggtcgg ttacggtttc
     7381 gggttctgtg atataataga agtctcccgg gaataagcat ttaatggtat tcaggttgcg
     7441 cctcataaaa accgggctgt cgggaatgct cgactgatag cgtccgcagg caaaaaagcc
     7501 caaaaacaac aacaaagaga ttattttttt cactacagct ttttttaatg ccggcaaatc
     7561 atacctttat gcggctgaat ttatcaggtc tcaaagatac atattcctcc ggccacttcg
     7621 gatgttttgg ccaaaaaata aggtataact catttaaatc cattattttt gtgctcaaaa
     7681 aattgaaaaa atgaaaaaat cggttcttat tttatgtaca cttatgatga tctcctgcgc
     7741 caaaaagcaa ggtaatcctt tttttgaaga ctacgacacc ccttatggtg ccgtaccttt
     7801 cgataaaatc ggtatagaac attacatgcc ggcttttgtg gaaggcatga accggcaaaa
     7861 gctggaaatc gacgccatag ccggcaaccc cgaagcacct actttcgaga ataccattgc
     7921 agctatggac tattcgggcg aattgctcaa taaagtatct tcggtgtttt ttggtttaat
     7981 ttcgagccac accagcgatg agatgcaggc cattgctcag gaattgagtc ccctgctctc
     8041 ggagcacagc gacaatattc gcttaaatgc cccgcttttt aagcgcatcg aaagcctcta
     8101 caacaataga gacagcctga atctgaacac cgaacagcag cgtctgctcg agaaatacta
     8161 caaaaacttt gtgcgctccg gggctgctct cgacgaaagc cggcaggaac ggctgcgcga
     8221 aatcaacaaa gaggaggctc tgctcacctt gcagtattcc gataatgtgc tcaaagaaac
     8281 caacgactac cgcctggtga tcgacaagga agaagatttg tccggcttgc cgcagacggt
     8341 ggtggaagcc gctgccgaag ctgccgccgg ggccggtctt gagggcaaat gggtatttac
     8401 cctgcacaat cccagccgcc tgcccttttt acaatacgcc gacaatcgcg aacttcgcga
     8461 aaagatactc aaagcctatt gtatgcgggg cgacaacaac aacgaagccg acaataaaga
     8521 aatagtccgc aaactggtca acctgcgtgt agaaaaggct caactgctgg gttacaacac
     8581 ttttgccgaa tacgtgctgg aagaaaccat ggcaaaaaat ccgaaaaatg tgtacgactt
     8641 tttagccggc atctggccac gagccctgcg gcaagcgaaa aaagaagtaa cagaactcca
     8701 gaaacttatc gaccgcgaag gcggtaaatt caaactgcag gcctgggatt ggtggtatta
     8761 cgccgaaaaa ctgcgcaaag aaaaatatca actcgacgaa gaagagctga aaccttattt
     8821 tgagttgcaa aacgtgcgcc aaggtttgtt tacggtgtgc aacaaattat tcggcatcaa
     8881 cctaactccg gtaaacaacg tgcccgttta tcatcccgac gtggaagtct tcgaggtaac
     8941 cgacgccgac ggctcccacc tgggtttgtt ctactcggat tatttccccc gcgaaagcaa
     9001 aaggggcgga gcctggatga gtaatttcag ggaacaatac gtcaaaaacg aacaaaacgt
     9061 caggccgcat atttacaatg tgggcaattt taccaaaccc agcggcaaca ctccctcgct
     9121 gctcagcctc gacgaagtgg aaaccatgtt tcacgaattc ggacataccc tgcacggaat
     9181 gctgagccaa tgcagctacc ccagcctgaa cggcactaac gtgacacgcg actttgtgga
     9241 gctcccctcg caaattatgg aaaactggtg tacgcatccc gaagtgctca aaatgtatgc
     9301 ccggcattat aaaaccggag agcccatgcc cgaagagctg atcgaaaaaa tacagcgcgc
     9361 cgctactttc aaccagggct ttattacaac cgaactgatg tcggccgctt acctcgatat
     9421 gtactggcac gatctgagcg aacaaaaaga ctacgatgta aatcaatttg agaccgactg
     9481 cctcagccgc atcggactga ttcccgaaat cgtggtacgc taccgcagca cctatttcaa
     9541 ccacatattc aacaacaatt accacgccgg ttattattcc tatctctggg ccgaagtact
     9601 ggacgcggat gcttttcagg cttttgtcga aaacggcctt tttgaccaga aaacggccac
     9661 agccttcaga caaaacattc tcgagagagg cggcaccgac gacccgatga ccctttaccg
     9721 gaaattccgg ggagccgatc ccgatccaaa ggctttgctc gaacgcagag gcatttaagt
     9781 ggcatgaata aaacaaaaga cttgaaatta aatgccttta agttctttgt gaataaaaaa
     9841 aagtctatct ttgcgcccta aattaaaaat caaggaataa ctaataaaat atccaacaac
     9901 aatgcaaaac aaaggatttg taactctttt tgcagttctg ctcgccctgg tcagcctcta
     9961 ttatttgtct tttaccgcag cggttaagaa caccgaaaag aaagccaaag aatacgcaca
    10021 aggcgaccct gttctggaac gtaattttct ggattcgatg gcccaggaaa aaatttattt
    10081 gggcaacacc tacagggaat gcctcgaaaa agaaatcggt ctcggtctcg acctcaaagg
    10141 aggtatgaac gtcgttttgg aactgtccgt tcccgatgtg ctcagagctt tgtcgggcta
    10201 caataccgac gaaaacttca acaaggcatt ggcgcaagcc agcctccgta tgagagaaag
    10261 ccagcgtgat tacctggatc tttttgtcga agagtacaag gccatcgacc cgggggcccg
    10321 tctctcggcc attttcagta cgttcgaaat gaagggacgc atcgacccct ccagttccga
    10381 cgaaagagta atacaggtat taagagagga tattaagagc gccgtcgaca actcatttaa
    10441 cgtattgcgc acccgtatcg accagttcgg ggtggtccag cccaatattc agcgtctgga
    10501 aaccggcggc cgtattttgg ttgaattgcc cggtattaaa gaaccagaac gtgttcgtaa
    10561 actgcttcag ggttcggcca atctggaatt ctgggaaacc tatgaattga gcgaattgta
    10621 tcaggctttg atagaggcca acaacgccgt tcgcgacatg attgcggcca ctgagcctca
    10681 aaaagaacaa gttgccgaag ctgctccggt agaaaaagcc ccggtagaac aagtaagcga
    10741 caccgccaaa accgaggaag aacgcctgct ggaacagctg gctgccgaaa ccgaaatcca
    10801 aacggacgaa cagagcctgg aagaatggaa aagagaatat cccttgtttg ccgtattgca
    10861 gattaaccaa tctcagcaag gcattggcgc cggcccctgc gtaggttatg ctctgggacg
    10921 cgataccgcc aaagtaaacg aatacctcaa cagccgcaac gtaagggaat tgttgccccg
    10981 cgatgtgaaa tttttatggt cggtcaagcc cattaacgaa aaaacacctc tttttgagct
    11041 gatcgccata aaagtgacca atcgtgacgg tcgcgccgcc ctcgaaggcg atgtgatcac
    11101 cgatgccagg gacgacttcg aacaaatgag cggctcggcc gtggtctcca tgtcgatgaa
    11161 tgccgaaggt tccaaaactt gggctcgcat taccaaagac aacataggca agtgcgttgc
    11221 cattgttctg gataattacg tgtattccta tccccgggtt aacaccgaaa tcaccggcgg
    11281 ctcctcgcag attaccggaa actttacctc ggccgaagcc aaagacctgg ccaacgtatt
    11341 gaagtcggga aaaatgccgg ctcctgcccg cattgtgcaa gaagacatag taggtccttc
    11401 gctgggtaaa gaagctataa agtcggggtt gatctctttt gcgattgctt tcgcaattgt
    11461 gctgatctac atgatctttt tctacggaat cattcccggt ctggttgccg acttcgccct
    11521 tttgtgcaac atcttctttt tgatgggtat attggcctct ttccaggcgg tgctgacact
    11581 gcccggtata gcgggtattg tgctgaccct cggcatggcc atcgacgcca atgtgcttat
    11641 aaatgagcgc atcaaagaag agctggcggc gggtaaaagc atcaaaaaag ccttgcccga
    11701 cggatacaag aatgccttct cggccatctt cgactccaac cttaccacct tgctgaccgg
    11761 tataatactc tttatactgg gaacgggacc catcaaaggt tttgccacta ctttgagcat
    11821 cggtatcctg acttcgttct ttagtggagt ctttattacc cgcatcatct ttgaacgtct
    11881 gctggccaag gaaaaactaa ataatatcac ttttaccacc aagttatcga aaaatatttt
    11941 aaggaatccc caagttaatt ttttgggcct gcgcaagaaa ggctacatcg ttgcgggaat
    12001 tttggtcttg gcgtgcattc tgtcgtggag cacacgcgga ttgagtcagg gtatcgattt
    12061 ttcggggggt cgcaattacg tagttcgctt tgaccaaccc gtttcaacgc aagacgttcg
    12121 caacagcctt gatggcattt tcgaggatgc ctcgctttcg gtaatcacta tcggctccga
    12181 aaaccaggtc agggtaagta ccaactacaa gataaatgaa acctacgatg aactggatgc
    12241 tgaaatcgac agcatactgt tcaccggctt aaaagactac ttgcccgaaa ataccggttt
    12301 ggaatccttt gtcgaagact acatcgtatc ttcgcaaaaa gtcgggccta ccgttgcctc
    12361 cgacatcaag atttcggcta tatggtcggt agtggctgct ctgctggtaa tcggcttgta
    12421 catcctgttg cgtttccgca atgtggcctt cagtgtcggt gttgtcgctt ccttagcctt
    12481 cgttaccata tttattattg gcttgtattc tatgctgtac agcgtgatgc ctttctcgtt
    12541 ggaaatagat caaaccttta ttgcggctat cctgacggtg attggttatg ccgttaacga
    12601 ctcggtggtg atcttcgacc gtatccgcga aacaacgggc ctttatccca agcggcataa
    12661 gcgactcacc atgaactcgg ctatgaatac caccttgttg cgtactttca gcaccgcgtt
    12721 tagtaccgcc cttgtactgc tggccatcct tctttttgga ggagacacca tccgcggttt
    12781 tgcctttgcc atgttgatag gtgtggtatt cggaacactc ggaacgctct ttattgcctc
    12841 ccctgttgct tacgatattc agcgtaagag ggaaaannnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnttta aggggtaaca actttaactg cacattgctc gcagccgagg
    13021 tctgcaacat taagtaatct tcggtgagta ttttcaattc catctctgaa cctaataaac
    13081 caagcattct cgctgttttg ccgctgttaa agaaatcgac catatccaac ttcatggcgg
    13141 tgttcagccc cggcagcaaa tcggcgggca tatctttgaa acattccact acacttcgac
    13201 tccaaacgcc cggtacgatg aagcaggtaa ttaacaacag taaaacgact tttctcatac
    13261 ctaaacaacg attacttaga gcccaaatac aggcaacaca ttccaataaa cacgcccaac
    13321 aaaatccagg ctttggcctt aatgtttttg tctttaaaga acaaagcccc taccacaaaa
    13381 ggaatcaaca cccccgaacg gcgtatgggc gaaatcaccg aaatcaatgc tcccggcctg
    13441 ctcagggcgt aaaagtacaa aaagtctgcc aggcagagaa acacggctaa acctactatg
    13501 gaccagcgaa agtcgaaggc ctgttccttg cctctttttc gccggtgcat atagatgagc
    13561 aacaggggca acatgatgaa tatctgataa atggtgtacc aaacctgcac agtcatgcgg
    13621 tcgaaattca ccatcatata tttatcgtag agtccgctga ccgatccgat caacacggat
    13681 aagaacaaac accagatcca gggattgcgc ctaaaagaaa taccttcttt tttgcccaca
    13741 taagagtaca gaaaaatact gatgattgaa accagcaccc ccagccattg ccaaaggttg
    13801 agccgctccc caaaaaccat aaaagcaccc atcagcacaa aaaccggctg agtggattta
    13861 atgggagata caatggttaa aggtagattt ttgatggcat aataggcagc ggcccacgag
    13921 cttaaaacga taaacgattt aagtaaaata aaaagatgtg ttttcgcgtc tgcccgcggc
    13981 acaaaaaaca aggtgttttc gaggctttgc ggagcccact ccgaaagcag caacaaaggc
    14041 aaaaaaaaca gggaacaaaa aaccgtggtc aaaaacaaga ccggtacaac ggcattttcc
    14101 tttaacgaca gttttttata tacttcgtaa acccccaaca agagggaaga agcaaaagta
    14161 gcccacaacc acataagtca aaaatcaacc aattaaatac aaatcatcac tatatttcac
    14221 ttccgtcaga tacagagcgt gtgccggcat ggatgtgcct gccctgcaac ggtcgcgggc
    14281 ctcgataata cggcaaaatc cctcttcgtc tatcctaccc ttgcccactt cgagcaaact
    14341 acccactatg gctcgtacca tattgcgcaa aaaacggtcg gcctgtatgg tgaaaatcag
    14401 ctcactcccg cgacgttccc aacgagcatg tgttaccttg caaataaagg ttttgacatc
    14461 ggtatgcact ttgctgaagc tggtaaaatc gctgtactcc aacagacggt cagcagcctt
    14521 gttcatagcc tccacatcgg gttcgtaata caaacgaagg ctgtaatctc tcaggaaagg
    14581 atctttttgc agtattattt tatactgata agttctcgag atggcgtcga agcgggcgtg
    14641 agcgccgggc cgtaccctgc ggagggaacg aacggcaata tcggccggca gcattcggtc
    14701 cagtttatta gccattgcat ccggattttc gatgggatag gccgtatcga aatgcgccgt
    14761 catagctaag gcgtgtacac cggagtcggt gcgtcctgca gcaataatat ccaggtcttt
    14821 ttcgaataaa gtgcgcagca cagaggtcat aacttcttgc acgctaatgg cattgggttg
    14881 tatctgccat ccgtggtaag cgctgccgtc gtaagaaaaa ttcagaaaaa aacgttgcat
    14941 taccgggaga ggaaaaattt ggctgcaaag ttagtcttta ttttttattc caatcgaatt
    15001 gagtactttt gcgcaaaatg ttaggccccg ggtgaatgaa cgaaaaaatc ctgtttctgg
    15061 aacaagtaga cccctctgtg ttttgcggag tcaacaacag taatatcaaa ctactcaaag
    15121 atttatatcc caaactacgt atcattgccc gcggaaatgt gattaagatt ttaggcgaag
    15181 aagccgaaat tgcccgattt gaacaactaa tcaatcactt gcaagactac ctaaacaaat
    15241 acaacctgat caaccaggat gtcattaccc aactcaacca gggcaagcct ctttttgaac
    15301 ccaaacagga aaatcttatt ctttacggtg tcaacggcaa acccatcctg gccagaacca
    15361 tcaatcagca aaagctggtt aaagcttacg aaaaaaacga cttgttgttt gccgtagggc
    15421 cggccggcac aggtaaaacc tatactgcca tcgccctggc cgtaagggcc ttcaaaaaca
    15481 aagaagtacg taaaattatc ctgagccgtc cggcggtcga agccggtgaa aaactgggtt
    15541 ttctgcctgg cgacatgaaa gaaaaggtcg atccctattt gcaaccgctt tacgatgccc
    15601 tcgaggatat gattccgccc tttaagctca gggaatatat ggaaaacaag cagatccaga
    15661 ttgctcccct ggctttcatg cgcggaagaa ccctgaacga tgccgtcatc attttggacg
    15721 aagctcaaaa caccaccggc ttgcaaataa aaatgtttct tacccgcctg ggcctgaacg
    15781 ccaaaatgat agtcaccggc gacctcaccc aaattgacct tccggcggca acgacttcgg
    15841 gtctggttga atcgctcaaa gtgttgcacg gcatcgaagg catcggcaaa gtggagttcg
    15901 atgtacggga catcgtcagg cataagctgg ttaaacatat agtgaaagca tacgaagact
    15961 accaacagga acaaaaaaaa ctaaaaaaca cccggaccga ccggaataac cccaacgaat
    16021 aaactctagc tttatgaaac aggctttggt aaaaacagac ttcaattttg acggacaaat
    16081 atctgtttat cacggaaaag tccgtgacgt atacaatatc gaggacgaat tgttggttat
    16141 ggtggtcagc gaccgcatat ccgccttcga cgtggtgctg cccaaaggga tcccttacaa
    16201 gggacaggtg ctcaaccaaa ttgccgctaa atttctggac gccacctccg atatcgtcaa
    16261 aaactggaaa atagccgtgc ccgaccccat ggttactgta ggtgtcaaat gtgaacccta
    16321 cgaagtggaa atggtgatac ggggctatct cacgggccat gcctggagag aatacaagtc
    16381 cggcaaacgc agcctgtgcg gtattcaact gccagagggc atgaaagaga acgaacgttt
    16441 tcccgaaccc atcatcaccc ccaccaccaa agccaaagag gggcacgacg aggatatttc
    16501 cagagaagaa atcatagcct ccggcctggt cggcaaagcg gaatacgaaa aattggaaga
    16561 atatacccgg gctattttta aacggggcac cgaaatggcc gcccaaatgg gcctgatcct
    16621 ggtggacacc aaatacgaat tcggcaaaaa agacggcgag atcattctga tggacgaaat
    16681 ccacaccccg gattcttcac gctattttta tgccgatggc tacgaagcaa agctggcagc
    16741 caacctgcct caaaaacaac tttccaagga gtttgtccgc caatggctca tcgaaaacgg
    16801 gttccagggt aaaagcgggc agcaggttcc ctacatgagc gacgaatact gccggagcgt
    16861 ttcggagcgc tatatcgaac tttacgaaaa agtgaccggc gaaaactttg tacgtgcgga
    16921 aaccgaagac gtaaaagccc gtatcgagcg caatgttacc caatttttga aacaatatcg
    16981 ttaattatgt ccgaccagcg ctatcagctc agaggggttt cagccggcaa agaagacgta
    17041 caccgggcta ttaagaatat cgacaagggt ctttatccca aggccttctg caagataatt
    17101 cccgatatac tgggcggcga tccggcttat tgcaacatta tgcatgccga cggagccggc
    17161 accaagtctt cgctggccta cctctattgg aaggaaaccg gcgatctttc cgtttggaaa
    17221 ggcattgccc aggatgcgtt aataatgaac atcgacgact tgctttgcgt cggagcctgc
    17281 gatcatatat tggtttcttc taccataggc cgcaacaagc atttgattcc gggcgaagtt
    17341 atttcggcca ttatcaacgg caccgaagag ttgttggaag aactccgttc catgggtctg
    17401 ggcgtctatt ccaccggagg cgaaacagcc gatgtgggcg acctggtgcg caccattata
    17461 gtggacagta ccgtaagttg ccggatgaaa cgagccgatg tgatcgacaa cgccaacatc
    17521 aaaggaggag atgtcatcgt agggttggcc tcgtttggac aggctgttta cgaaaaaagc
    17581 tacaacagcg gcatgggaag caacggactc acttcggcca gacacgatgt gtttgctcat
    17641 tatctggctc aacaatatcc cgaaagctac gaccccaata cacccgaaaa attaatttac
    17701 tccggacagc tcagactgac agaacctgtt cccggcacgg gaatgactgc cggacaactg
    17761 gtgctttccc ccacccggac ctatgctccc gttatcaagg cagtactgaa cgaacttaga
    17821 ccccggatcc acggcatggt gcattgtacc ggaggtgctc aaaccaaagt gttgcatttt
    17881 gtcgaaaata aaagggtggt caaagacaag cttttcccct taccgcctct attcaaactg
    17941 atacaggaac aatcgggcag cgattggaaa gaaatgtaca aagtcttcaa catggggcac
    18001 cgtatggaaa tctatctcga tccttcggat gccgaacgag ttatagaaat agccgccggc
    18061 tacggcgtcg aagctcaaat aatagggcga attgaagaag ctcgggccaa tcaattgata
    18121 atcgaaagtg aatacggccg tttcgaatat tattagccgg aatttgtaac gaaaacagcc
    18181 tttagcacat ctatgggctg gcacccgcac aactttttct tttttttctt ttaagcaaat
    18241 aaatgaccga acattcctta ctggacagac tcgactggct acaaagccgt tttgaggaag
    18301 tatccaccct gattgcctct cccgacgcgg tttcggatat gaaacgctac gtcaggctca
    18361 acaaagaata cagggatctg gaacaaattg tcctggctcg ccgggaatac ctgcaactca
    18421 tcaacaacat aaacgaagcc aaagccctgc tcgagcacga aagcgatgcc gaattgcgcg
    18481 agatggcccg cgaggagctg aataaagaaa cagccagaca gcctcaactc gaggaaaaga
    18541 taaaacttat gctgattccc gccgatcctc aggatagtaa aaacgccatc gtcgaaatcc
    18601 gtggaggtac cggcggcgac gaagctgcca tctttgccgg cgatttattc aggatgtaca
    18661 gtaaatattg tgaatccaaa aattggaaag ttgaggtaac caactccaat ccgggtactt
    18721 cgggaggtta caaggaaatc atttttaccg tcagtggcga gcaggtctac ggaacgttga
    18781 aatacgaatc gggggtgcat cgcgtacaaa gagtacccct gaccgaaacc cagggcagag
    18841 tacatacttc ggcagccacg gtggctgtgt tgcccgaagc cgaagaattc gatgtagaac
    18901 tgaatgaatc cgacattaag gtggatacct tttgttcttc cggacccggc gggcagtctg
    18961 tcaacactac ttattcggcc gtccgcctgc tgcatattcc caccggtgtt gtggtacaat
    19021 gccaggacga acgttcgcaa ctgaaaaaca aagccaaagc catgaacgaa ttgcgctcgc
    19081 gcctttacga catggaatac caaaaatacc tggaagagat ctcttcgaaa cgtaaaacaa
    19141 tggtttccac cggagaccgt tcagctaaaa tccgcaccta caattaccct caagcccgta
    19201 tcaccgatca caggatcaat cttacaatgt acaacctgag tgccgtactc aatggtgaat
    19261 tgcaggagat tatcgatcag ctaatcattg ccgaaaatgc cgaacgcctc aaagccggag
    19321 gttattgata cccatatatc atgaacagac aagaaattat ttcccaaatc aagaccaagg
    19381 ccaccttttt gtgtatcggc ctcgatcccg acatacaaaa aataccctca catcttctga
    19441 agggggaaga gcctttattc gctttcaaca aagccattat cgacgccact cacgaccttt
    19501 gtgttgccta caagcccaat cttgcttttt acgaaagctg tggtctcgag ggttggaaag
    19561 ctttcgaaaa aacggtccgt tacatccggg aaaaagatcc cacgcaattc ataattgccg
    19621 acgccaaaag aggagatata gggaatactg ccgaaatgta cgcccgcagc ttttacgaaa
    19681 gcctgcccat agacgccgtc accctggctc cttatatggg caaggacagt gttcagcctt
    19741 ttttgtccta tcccggcaag tgggcaatcc tgctggccct gacttccaac cggggttcgg
    19801 ccgacttcca gatgctggaa gacgcacagg gggaacgtct gtttgaaaaa gtgcttcgcc
    19861 gctcttccga ctggggcgac gaattttcga tgatgtacgt ggttggtgcc actcaaggca
    19921 gtctgcttca ggatgtaaga aaactggttc ctcgtcattt cctgctggtt ccgggcgtag
    19981 gaactcaggg aggcagcctg aaagaagtgg cacgctatgc gatgaacgac gactgcggat
    20041 tgttggtaaa cgcctcgcga caaatacttt atgcttcaaa agaagaagaa tttgctcagg
    20101 cagcccgcca agaagccatg aaacttcagc aagaaatgca aacttatctg aaggctttat
    20161 ggggatgaaa caaaaaataa taaacgatcc tgttttcggg tttatcaatc tccccacagg
    20221 tttcctctac caggtgtacc agcatcctta cctctaccgt ttgagcagga tacaacaact
    20281 gggcttgtcg ttcctggtgt acccgggagc caggcattgc cgttttcaac acatcaccgg
    20341 agccctgcat ttggcaagcg aagccatacg ccaattgcgc ctcaaaggca atgagatcac
    20401 cgaggccgaa gcagaaggca ccgtggctgc cattatgttg cacgacatcg ggcacgggcc
    20461 tttttcccat gtgctggaac cggttttgtt caacgggctt tcgcacgaaa aaatttctct
    20521 cttgctgatg gagcgtatga acaaagagtt caacgggaaa ctcgatatgg ccatcgccat
    20581 attcaaaggc agctatccca agaaattttt acatcagctt atctccagtc aattggatgt
    20641 ggaccgcttg gattatttaa ggcgggacag tttttttacc ggagtagtcg agggcaatat
    20701 cggctcttcg cgcatcataa aaatgctagc cgtatgcgaa gacaggctgg tggttgaggc
    20761 caaaggcatt tattccatcg aaaatttcct gatggcccgg cgtttgatgt attggcaggt
    20821 ttaccttcac aaaacttcgc tggcagcgga aaaaatgttg cacaatttgc ttcacagagc
    20881 caaagtgctc aagcaggcag gccaggatct gacttgcagc ccggccttgt cttttttcct
    20941 ttccggtaaa cttaaaccgg gcgaaatcag cgacgtcgct ctggatcatt tcatagcatt
    21001 ggacgatacc gacatttggt cggccataaa gaactggcaa aaccatccgg acgtggtttt
    21061 gtccactttg tcccggaatt ttcttaaccg taaattgttt aaaattgaga tatccgaaca
    21121 ggaaacgccg ccctcccgcg ttcaggaaga cttgcaacgc attgccttgc aattgaatat
    21181 cggtattgag gaggcaaggt attttatttc gctcaataag gtaagcagcc atatttacga
    21241 cgatgcggac tacggcatcg atattctgta caacgacggc aggatacgcc ctataaccca
    21301 ggcttcggac attcttaacc tcgaagttct atcaaaaaaa gttagaaaat atgcctattc
    21361 ctacctgaga aaagtttgat ctgttgaaca agccaaatta aaagctatcg attattagta
    21421 atattttata tctttgcgcc aaatttttaa catggatttt acagccagcc aaat
//
LOCUS       DAOSTD010000026        20744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_2759_length_20744_cov_14.734835, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000026 DAOSTD010000000
VERSION     DAOSTD010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20744
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2759_length_20744_cov_14.734835"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLI37_02990"
     CDS             complement(<1..448)
                     /locus_tag="PLI37_02990"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOQ58018.1"
                     /translation="MKKTRPIIIRILACIIGLPVLVFIAVLVLLLHPKSLHSIVQTQA
                     EKHLNCPLEIGSIDLDLFDNFPKMELSIKELNLLNPFPGTPNDTLLGLKQIKTSVHLG
                     KLLSQRALVIDTFLVDRLNLYAYIDSNGQSNFAVLNLESDPEDTTAL"
     gene            complement(480..854)
                     /locus_tag="PLI37_02995"
     CDS             complement(480..854)
                     /locus_tag="PLI37_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58019.1"
                     /translation="MKKSQHKTSLFFWTIFFVSYVSTSCITESEYDSSLLIGKWVSGT
                     EYYRYNEDGTGLTWDTADDVTEQEAQAFTWTLVYSTLTHMHILEVGGVVPKVYTVTEL
                     TATSLKYEDEFGKTYSFTKSSS"
     gene            complement(864..>1079)
                     /locus_tag="PLI37_03000"
     CDS             complement(864..>1079)
                     /locus_tag="PLI37_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HOQ58020.1"
                     /translation="LSFEQDPELLVLNGRYGPYISKKGQNYKIPKKTDPATLSYQDCL
                     DLIEKQAKETNIKKGRKMIRKSPPKKK"
     assembly_gap    1080..1179
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1180..3376)
                     /gene="topA"
                     /locus_tag="PLI37_03005"
     CDS             complement(<1180..3376)
                     /gene="topA"
                     /locus_tag="PLI37_03005"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOQ58021.1"
                     /translation="MQKNLVIVESPAKAKTIEKFLGKDFKVMSSFGHIRDLRKKDLGV
                     EIENNFKPVYEISPEKKKLVGELKSAAQKAETIWLASDEDREGEAIAWHLSEVLNLKE
                     ENTKRIVFHEITKEAIENAIKKPRNIDLNLVNAQQARRVLDRIVGFELSPVLWKKIKP
                     ALSAGRVQSVAVRLVVEREREIQQFNSESFYKLSLVFLVPNVDGSITELKAEFPEKIE
                     QKETALALLELLKTATFKVEDISTKPLRRSPAPPFTTSTLQQEAARKLGFSVAQTMRV
                     AQSLYESGKITYMRTDSVHLSSLAINTAKAEITRLYGEDNVKTRQYQTKSKGAQEAHE
                     AIRPTYMSVHSVDASGPEKKLYELIWQRSIASQMADARLEKTTITISHSNGNQLFTAS
                     GEVVTFEGFLKVYEYPDKENEAENTGSKLLPSVQLQQTLNLDTAQAVQRFSQKPPRYT
                     EASLVRKLEELGIGRPSTYAPTISTIQQREYIEKGNREGEKRQYNLLELSKTRQINDK
                     TKTEMSGVEKAKLFPTDVGMVVNDFLLESFPYIMNYQFTANVEKYFDQIAEGELEWVE
                     LIDQFYKDFHPAVQEATAYSGPKVGSRLLGKDPKSGRPVSVRIGRFGPLVQIGTAEDE
                     EKPLFASLRKEQSMDSINLEEALALFKLPRELGSFEGKSLSVGSGRYGNYIRHGNQFV
                     SLPKEFDPLEVSLDQAIQLVKDKREEDRKKIILSFEQDPELLVLNGRYGP"
     gene            complement(3560..4078)
                     /locus_tag="PLI37_03010"
     CDS             complement(3560..4078)
                     /locus_tag="PLI37_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOQ58022.1"
                     /translation="MALIKSVRGFDPVIGPNCYLAENASIIGDVVLGEGCSVWFNTVL
                     RGDVNSIRIGNNVNIQDGSVLHTLYQKSVIEIGDNVSIGHNVTIHGAKIEKLALIGMG
                     AVVLDHAVIGEGAIIAAGSVVLSGTRVEPYSVYAGVPAKFVKFADAEQTNTINKRISD
                     NYRFYASWYEEE"
     gene            complement(4080..5867)
                     /locus_tag="PLI37_03015"
     CDS             complement(4080..5867)
                     /locus_tag="PLI37_03015"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOQ58023.1"
                     /translation="MYNSIPKRLAALRNLMETNAIEACIIPTSDSHQGEYVSSHFKFR
                     KYLSGFTGSAGTLVVGKDKAGLWTDSRYYLQAEQELRGSGIDLFKTTLPETPGIPEWI
                     AEMNYRSAGIDGEVFAAREVLKMIQVFNKHGVRLVDDFKPYTRVWPDRPDLPDSPFYV
                     FPVGYAGKSVKEKLSELRAELSCQGADYLPVSGLDDIAWLFNLRGSDVDFNPVGLAFA
                     LIGPDIANLYTDKNKIDESTLAYLKENNIQLRDYSALYADLSKLPAGSGVFTDTTRIN
                     YFIYKHIPSNCRRVEGTAPTTALKSIKNPVEIAGFRTAMQKEGVALVRFWRWFEAALE
                     QGKELYETDLVRKMSEFRLEQDHCKGDSFYSIISYNEHGAIVHYSPSESTKARILPDG
                     YLLVDTGGQYLEGTTDITRTFSLYRGATPEEFKQDYARILKGNIALSECIFPTHTRGS
                     QLDIMARQFLWKDLLQYGHGTCHGIGHFLNVHEGPQSIRMEENPELIKAGMVMSNEPG
                     IYRTGKYGLRIENMMLVIEKGESEFGQFLGFETLSLCPLELKAIEKKYYTGQEIDWIN
                     AYHRFVYNRLSAFLSEEEKAWLALKTKEI"
     gene            6064..6255
                     /gene="rpsU"
                     /locus_tag="PLI37_03020"
     CDS             6064..6255
                     /gene="rpsU"
                     /locus_tag="PLI37_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOQ58024.1"
                     /translation="MIIVPVKEGENIERALKKFKRKFEKTGTVKELRNRQAFRKPSVV
                     KRQQKMKAVYVQQLQQNEE"
     gene            6352..7266
                     /locus_tag="PLI37_03025"
     CDS             6352..7266
                     /locus_tag="PLI37_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOQ58025.1"
                     /translation="MWVEQYIQYLRYEKNYSSHTVFAYKRDLEQFLAFASDTFQLEHP
                     SDIDADIIRAWMLALMEKGDTARSVNRKLSSLRSYWRYLLRQGLVTSNPTQKVLTPKI
                     KKALPSFLKEEELNDLLDKAREEGQTDSFTESRDNLILEMFCLTGIRRAELIGLKTGD
                     VDIGSASLKVTGKRNKQRILPFGTALKKSIENYLVLRSDYLDKLEDYPEVEPPFFINA
                     KGRALNPRRVYSLVNKKLAEVGNLSQKSPHVLRHTFATTLLNRGAELNAVKELLGHAS
                     LSATEVYTHTTFEELKKVYKQAHPRAEN"
     gene            7281..7580
                     /gene="raiA"
                     /locus_tag="PLI37_03030"
     CDS             7281..7580
                     /gene="raiA"
                     /locus_tag="PLI37_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOQ58026.1"
                     /translation="MEIRIQSVRFDATSALEKFIQKKVAKLAQYYEGILSAEVTLKVT
                     KPETVANKEALIRLNISGRDLFASKTCDTFEESIDEAVEALEKQLKRHKDKIRSK"
     gene            7696..7778
                     /locus_tag="PLI37_03035"
     tRNA            7696..7778
                     /locus_tag="PLI37_03035"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7730..7732,aa:Tyr,seq:gta)
     gene            7834..7906
                     /locus_tag="PLI37_03040"
     tRNA            7834..7906
                     /locus_tag="PLI37_03040"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7867..7869,aa:Gly,seq:tcc)
     gene            7910..7981
                     /locus_tag="PLI37_03045"
     tRNA            7910..7981
                     /locus_tag="PLI37_03045"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7942..7944,aa:Thr,seq:ggt)
     gene            8025..9212
                     /gene="tuf"
                     /locus_tag="PLI37_03050"
     CDS             8025..9212
                     /gene="tuf"
                     /locus_tag="PLI37_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOQ58027.1"
                     /translation="MAKEHFNRTKPHVNIGTIGHVDHGKTTLTAAITTILAKRGLSEV
                     KSFDQIDNAPEEKERGITINTSHVEYETENRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     IIVVAATDGPMPQTREHILLARQVNVPRLVVFMNKCDMVDDEEMLELVEMEMRDLLGF
                     YEFDAENTPVIRGSALGALNGVAQWEDKVIELMAAVDNWIQVPPRAVDKPFLMPVEDV
                     FSITGRGTVATGRIETGIIKTGEEVQIIGLGAKGKKSVVTGVEMFRKILDQGEAGDNV
                     GLLLRGIDKDEIKRGMVVTHPGKVTPHTSFKASVYILKKEEGGRHTPFHNKYRPQFYI
                     RTLDVTGEITLPEGTEMVMPGDNLEIKVELIYPVACSEGLRFAIREGGRTVGSGQITE
                     ILK"
     gene            9275..9350
                     /locus_tag="PLI37_03055"
     tRNA            9275..9350
                     /locus_tag="PLI37_03055"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9308..9310,aa:Trp,seq:cca)
     gene            9364..9570
                     /gene="secE"
                     /locus_tag="PLI37_03060"
     CDS             9364..9570
                     /gene="secE"
                     /locus_tag="PLI37_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOQ58028.1"
                     /translation="MKKFFKRVATYVKDAYNELVHKVSWPTPSELSNSAIVVLTASLV
                     IALIVFIIDLSLENLMTFIYEKVF"
     gene            9583..10125
                     /gene="nusG"
                     /locus_tag="PLI37_03065"
     CDS             9583..10125
                     /gene="nusG"
                     /locus_tag="PLI37_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOQ58029.1"
                     /translation="MSETSKKWYVLRAISGKENKVKEYIEASINNSSLGEHVSQVLIP
                     TEKVYQSRGGKKVLKERSYLPGYVLVEANLVGEVAHALRNVPNVLGFLGGMNNPQPLR
                     ESEINRILGKVDELQEAGDDISINYYVGENVKVNFGPFSGFSATIEEVNNEKKKLKVM
                     VKIFGRKTPLELGFMQVEKE"
     gene            10189..10632
                     /gene="rplK"
                     /locus_tag="PLI37_03070"
     CDS             10189..10632
                     /gene="rplK"
                     /locus_tag="PLI37_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOQ58030.1"
                     /translation="MAKEIAGQIKLQIRGGAANPSPPVGPALGSKGVNIMEFCKQFNA
                     RTQDKAGKVLPVVITYYSDKSFDFVVKTPPAAVQLLEASKTKLGSAEPNRKKVGEVTL
                     DQVRAIAEDKMSDLNCFTLESAMKMVAGTARSMGIRIKGELPTNN"
     gene            10645..11340
                     /gene="rplA"
                     /locus_tag="PLI37_03075"
     CDS             10645..11340
                     /gene="rplA"
                     /locus_tag="PLI37_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOQ58031.1"
                     /translation="MAKLTKNQKLALEKMEVGKAYSLREASELVKEITFTKFDASVDI
                     DVRLGVDPRKANQMVRGVVSLPHGTGKQVRVLVLCTPDKEAEAKEAGADHVGLDDYIE
                     KIKAGWTDVDVIITMPSTMGKIGALGRILGPRGLMPNPKSGTVTMEVGNAVREVKQGK
                     IDFKVDKNGIVHAAIGKVSFTPDQIRENAKEFILTLIKLKPTAAKGTYLKSIYLSSTM
                     SKGIKVDPKSIED"
     gene            11355..11876
                     /gene="rplJ"
                     /locus_tag="PLI37_03080"
     CDS             11355..11876
                     /gene="rplJ"
                     /locus_tag="PLI37_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOQ58032.1"
                     /translation="MKKEDKYIVVEQITQLVNEYEHFYVTDIASLNSEETSSLRRACH
                     KEDVKLYVVKNTLLKKALQGLETDYSSLFDTLKGNTALMFCNTGNAAAKLIKEFRKDK
                     SVPALKAAYVEECFYIGPEHLDTLIHIKSKNELIADIVALLQSPAKNVVSALQSGGTI
                     LHGVLKTLEEREN"
     gene            11949..12323
                     /gene="rplL"
                     /locus_tag="PLI37_03085"
     CDS             11949..12323
                     /gene="rplL"
                     /locus_tag="PLI37_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOQ58033.1"
                     /translation="MADLKAFAEQLVNLTVKEVNELAEILKNEYGIEPAAAAVAVAAG
                     PAAAGPAAEEKTSFDVVLKSPGAAKLAVVKAVKESCGLGLKEAKDLVDAAPSVLKEGV
                     TKDEADALKKMLEEAGAEIELK"
     gene            12431..16234
                     /gene="rpoB"
                     /locus_tag="PLI37_03090"
     CDS             12431..16234
                     /gene="rpoB"
                     /locus_tag="PLI37_03090"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOQ58034.1"
                     /translation="MSSTTDNQRINFASIKSPIDYPDFLEVQLKSFQDFLQLDTPPEK
                     RKNEGLYKVFSESFPITDTRNNFVLEFIDYYVDPPRYTIEECIERGLTYSVPLKAKLK
                     LYCTDPDHEDFETKFQDVYLGAFPYMTPKGTFVVNGAERVIVSQLHRSPGVFFGQSVH
                     ANGTKLYSARIIPFKGSWIEFATDINNVMYAYIDRKKKLPVTTLLRAIGFETDKDILE
                     IFNLAEEIKVTKTGLKKHVGRKLAARVLKSWVEDFVDEDTGEVVSIERNEIIIDRETV
                     LESSHIDDILESGTPTVLLHKEDPDMSDFSIIYNTLQKDPSNSEKEAVLYIYRQLRNA
                     EPADDSSAREVIQNLFFSEKRYDLGEVGRYRINKKLQLNTDMDTRVLTKDDIIHIIRY
                     LIELVNSNAIVDDIDHLSNRRVRTVGEQLNNQFGVGLARMARTIRDRMNVRDNEVFAP
                     VDLINAKTVSSVINSFFGTNALSQFMDQTNPLAEMTHKRRLSALGPGGLSRERAGFEV
                     RDVHYTHYGRLCPIETPEGPNIGLISSLCVFAKINKLGFIETPYRKVSNGIVEKEVVF
                     LTAEEEENLVVAQGNALLNEDGSFVRNRIKARLEADFPVVEPNKVDLMDVSPSQIASI
                     AASLIPFLEHDDANRALMGSNMMRQAVPLIRCEAPIVGTGLEKTLIKDSRTQLMAEGD
                     GVVEYVDATSIHIRYDRTEDEEFVSFDDAVKIYNVPKFQKTNQSTTIDLKPIVRKGQR
                     VTKDQLLTEGFATEKGELALGRNLKVAFMPWKGYNFEDAIVLNERLVREDIFTSVHVD
                     EYTLEVRETKRGMEELTADIPNVSEDATKDLDKNGIIRIGAHVKPGDILIGKITPKGE
                     SDPSPEEKLLRAIFGDKAGDVKDASLKATPSLSGVVIDKRLFSKAIKNRKTRLTDKAI
                     LPKLDEEFEEEVAAIRAVLLEKLWVLVSGKTSQGIKDNLDTEVIPKGVKFTQKILNEI
                     NYSSIQLSKWTSDNHKNDMIRDLIINYLKKEKELDAKLKRKKFNVTIGDELPAGIIQM
                     AKVYVAKKRKIKVGDKMAGRHGNKGIVSRIVRQEDMPFMEDGTPVDIVLNPLGVPSRM
                     NLGQIFETVLGWAGQKLGVKFATPIFDGAELEDLEQWSDKAGLPRYGKCYLYDGGTGD
                     RFDQPATVGVIYMLKLGHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWA
                     LEAFGASHILQEILTIKSDDVAGRSKAYEAIVKGEPMPQPGIPESLNVLLHELRGLGL
                     SIELD"
     gene            16257..20513
                     /gene="rpoC"
                     /locus_tag="PLI37_03095"
     CDS             16257..20513
                     /gene="rpoC"
                     /locus_tag="PLI37_03095"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOQ58035.1"
                     /translation="MALKKDIKTKTNFSKISIGLASPEEILELSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIQ
                     LVVPVVHIWYFRSLPNKIGYLLGLPTKKLDSVIYHERYIVIQPGILEEEEGVKVNDLL
                     SEEEYLALMDKLPRENQMLEDSDPQKFIAKMGADAVHDLLSRLDLDALSFDLRHRADT
                     DTSMQRKNDALKRLQVVEAFRAARHRNKPEWMVIKIVPVIPPELRPLVPLDGGRFATS
                     DLNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKSSAIKTDSN
                     RPLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKDMAAELYKP
                     FIIRKLIERGIVKTVKAAKKFVDRKDPVVWDILEHVMKGHPVLLNRAPTLHRLGIQAF
                     QPKMIEGKAIQLHPLACTAFNADFDGDQMAVHLPLGNEAIAEAQLLMLSSHNILNPAN
                     GAPITVPSQDMVLGLYYMTKERIGAQGEGLTFYSPEEAKIAYNEKKVSLHAVVKVRIE
                     GELIQTTIGRILVNEYVPEEVGFVNTVLSKKELRNVIGKVIASCGVARTAQFLDDIKD
                     LGYSMAFQGGLSFNLDDVIVPDEKTELIQGGYEEVEAIMEEWRQGFITPNERYNKIID
                     VWTIVNNKLSNILIKQLAEDDQGFNSVHMMMDSGARGSKEQIRQLSGMRGLMNKPQKF
                     GAEGGQIIENPILANFKEGLSVLEYFISTHGARKSLSDTALKTADAGYLTRRLVDVSH
                     DVIVNEEDCGTLRGLTALEIKGVASLYERILGRVSVHDVFNPDTNELLVSAGQEITED
                     IAQKIQDSLVDRVEIRSVLTCESKKGVCAKCYGRNLATGRMVEKGEAVGVIAAQSIGE
                     PGTQLTLRTFHAGGVAKSENVENKLVAPYDALVEIDELRVVPSFEDKNVRIVVGRYAE
                     IHLIDPVTNMVTTHNLSYGSRLYVDPGDMVKQGDLICEWDVYNAVIICEQSGKIEFES
                     IEEGVTYQTYMDETTKIKDKIIIESKDRSHVPTIHIMDDKGQRLYSYNLPVGAHLLKE
                     SGDRIEAGQVLVKIPRAIPKSGDITGGLPRVTELFEARNPSNPAVVSEIDGDVIELGR
                     IKRSNREIVIQSKTGEIKKYLVPLSKQVLVQLNDFVRAGTPLSEGSITPADILAIKGP
                     TAVQEYIVNEVKNVYRMQEVKINDKHFEVIVRQMMRKVEIVDAGDTHFLEQQEVDKNE
                     FMEENDRIWGKKVVLDPGDSATLKAGMIVTARKLRDENSSLKRRDLRLVEVRDAIPAT
                     SNQILQGITRAALQTSSFMSAASFQETTKVLNEAAIQAKVDRLEGMKENVITGNLIPA
                     GTGQREFEKLIVGSREEFERIEATRDSLTQDVVVEEAEHE"
BASE COUNT         5765 a         4437 c         4841 g         5601 t
ORIGIN      
        1 tcagggctgt ggtgtcttcg ggatcgcttt ccagattaag gacagcaaaa ttggactgtc
       61 cgttgctgtc gatgtaagcg tagaggttta ggcggtcgac gagaaaagta tcaataacca
      121 gtgcccgctg tgagagtaat ttccccaaat gaacagaagt ctttatttgt ttaagtccca
      181 gcagagtatc gttgggagta ccgggaaagg gattgagcag attcagttcc ttgatgctaa
      241 gttccatctt agggaagttg tcaaacagat ccaggtcgat gcttcctatt tctaagggac
      301 agttcaggtg tttttctgcc tgagtttgta ctatggaatg aaggcttttg ggatgcaaca
      361 acaagaccag taccgcaata aaaaccagca ccggcagacc gatgatgcat gccagtatcc
      421 tgattattat tgggcgggtc tttttcataa atcaggtact aagtctcgga aagctcctgt
      481 taagagctgc ttttggtaaa actgtaggtt ttaccaaact cgtcctcgta ttttaagctg
      541 gtggctgtaa gttcggtaac tgtatatact ttgggcacca ctcctccaac ctccaaaatg
      601 tgcatatgag tgagggtgga gtagaccaaa gtccaggtaa atgcctgagc ctcttgttct
      661 gttacgtcgt cggctgtgtc ccaggttagt cccgttccat cctcattgta gcggtagtat
      721 tcggtaccgg aaacccattt acctatcagc agtgaagaat cgtattctga ttccgtgata
      781 caggaagttg atacgtagct gacaaaaaaa atggtccaaa aaaataagct tgttttgtgt
      841 tgggattttt tcatagcgca ggtttatttc tttttgggtg ggctttttct gatcatttta
      901 cggccctttt ttatattggt ttctttggct tgtttttcga taagatcgag acaatcttgg
      961 tagctgagtg tagccgggtc tgtttttttg gggattttat aattttgtcc ctttttgctt
     1021 atgtaaggtc cgtagcgacc attgagaacc aataattccg gatcctgttc aaagctgagn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aggtccgtag cgaccattga
     1201 gaaccaataa ttccggatcc tgttcaaagc tgaggatgat ttttttccgg tcctcttccc
     1261 gtttgtcttt aaccagttgg atggcttgat ccaaactcac ttccagagga tcaaattcct
     1321 tgggtaagga aacaaattga tttccatgcc gtatataatt tccgtaacgt ccgctgccca
     1381 cgcttaagct tttaccctcg aaagatccca gttcgcgggg aagcttaaac aaagccaggg
     1441 cttcttccag gttgatactg tccatgcttt gttcttttcg cagggaggca aacaaaggtt
     1501 tttcttcgtc ttcggccgtg cctatttgta ccaggggtcc gaaacgacct atgcgtaccg
     1561 ataccggacg gccgctttta ggatctttac ctaaaagccg gctgcccact ttgggtccgg
     1621 aataagcagt ggcttcctgt actgccggat ggaaatcttt gtaaaattgg tctatcaatt
     1681 ctacccactc caattcgcct tcggcaattt ggtcgaaata tttttccaca ttggccgtaa
     1741 attggtagtt cataatgtag gggaaagatt ccagaagaaa atcgttgacc accataccga
     1801 catcggtggg aaagagtttg gctttttcta ctccgctcat ttcggttttg gtcttatcgt
     1861 tgatttgtct tgttttagac agctccagca ggttatactg gcgtttttca ccctctcggt
     1921 tgcctttttc gatgtattcc cgttgttgga tggtggaaat agtcggagcg taggtcgaag
     1981 gccttccgat gcccaattct tcgagtttac gaaccaaaga ggcttcggta tagcgtggcg
     2041 gtttttggct gaaacgttga acagcctggg cagtatccaa attcagtgtt tgttggagtt
     2101 gaacagaggg cagcaattta ctgccggtgt tttcggcttc attttctttg tcgggatatt
     2161 cgtagacctt cagaaaacct tcaaaagtga caacttctcc gctggcggtg aacaactgat
     2221 ttccattgga atgactaatg gtaatggtgg ttttttccag acgtgcatcc gccatttgag
     2281 aggcgatgct cctttgccaa atgagttcat ataatttttt ttcgggcccg gaggcatcaa
     2341 cgctgtgtac actcatatag gtcggacgta tggcttcgtg cgcttcctgg gcacctttgc
     2401 ttttggtctg gtattgcctg gttttgacgt tgtcttcacc gtacaggcgg gtgatttcgg
     2461 ctttggcggt attgatggcc agcgaagata gatggaccga gtcggtacgc atataggtga
     2521 tttttcccga ttcatagagc gattgggcta cccgcatggt ctgagctacc gaaaacccga
     2581 gtttacgggc ggcctcctgt tgcaaagtag aggtggtaaa gggtggagca ggtgagcgcc
     2641 tgaggggttt ggtgctgata tcttccactt tgaatgtggc tgttttaagc aattccagca
     2701 aggcaagagc cgtctctttt tgttctattt tttcgggaaa ttcagcttta agttccgtta
     2761 tgcttccatc gacattaggc accagaaaaa caaggctcag cttataaaag ctttccgaat
     2821 tgaattgctg aatttctctt tcccgctcca cgaccagtcg aacagccacc gattgtaccc
     2881 ttccggcaga aagtgccggt ttgatctttt tccaaagcac cggcgataat tcaaatccca
     2941 caatgcggtc aagtacgcgc cgggcttgct gtgcatttac caggttgaga tcgatgttgc
     3001 ggggtttttt gatggcgttt tcgatggcct ctttggtgat ttcgtggaat acgatacgtt
     3061 tagtattctc ttctttaaga ttgagcacct cagacaaatg ccaggcgatg gcttcacctt
     3121 cgcggtcttc gtcggaggcc agccaaatag tttctgcttt ttgagctgcc gattttaatt
     3181 cgcccaccag ttttttcttt tccggagaaa tttcatagac cggtttgaaa ttattttcaa
     3241 tttcaacacc taaatctttt tttctcaggt cacgaatgtg cccaaaactc gacattactt
     3301 tgaagtcttt tcctaaaaac ttttcgatgg ttttggcttt tgccggggat tctactataa
     3361 ctaaattctt ttgcattaaa gagcgctttc aattaattcg gaactattta tccggaagtt
     3421 caacatcaca aaaaaaagag ggcttaaatc caattattca ccaattcaca gctgtaaaaa
     3481 cggcgcaaaa ttagtaaaaa cagttcagtg tcgggttatt tttctgaaaa tttatgcaga
     3541 aatcagcata tgcaagacct tattcttctt cataccaact cgcgtagaat cggtaattgt
     3601 ctgaaattct tttattgatc gtattggttt gttctgcgtc ggcaaacttg acaaacttgg
     3661 ccgggacacc ggcatagaca ctgtagggtt caaccctggt tccactgagc actaccgaac
     3721 ctgctgcaat tatggccccc tctccgatga cggcatgatc cagcactaca gcccccattc
     3781 ctataagtgc aagtttctca attttagctc cgtgtatggt aacgttgtga ccgatggaaa
     3841 cattgtcccc gatttctatg acagattttt gatacagggt gtgcagcacg gagccgtcct
     3901 gtatattcac gttgtttcct atacgtatgg aattgacgtc tccccggaga accgtgttga
     3961 accagacgct gcagccttcg cccaatacca cgtctccgat aatgcttgcg ttttcggcca
     4021 aatagcaatt cggcccgatt acggggtcga agcccctgac tgattttatt aatgccatgt
     4081 tatatttcct ttgtttttag agccagccag gctttttctt cttcgctcag aaaggctgaa
     4141 agccggttgt agacaaaacg gtggtaagcg tttatccagt cgatttcctg cccggtataa
     4201 tatttttttt caatggcctt aagttccaag ggacacagac taagggtttc gaaacccaag
     4261 aactgcccga attcggattc tcctttttca attaccagca tcatattctc tatacgcaga
     4321 ccgtattttc cggtgcggta aatgccgggc tcgttggaca tgaccatacc ggctttgatc
     4381 agttcgggat tttcttccat gcgtatgctc tggggacctt cgtgcacatt taggaaatgc
     4441 cctatgccgt gacaggtgcc gtgtccgtat tgaagcaggt ctttccagag aaactggcgg
     4501 gccataatgt ccagttgaga gcctctggta tgggtgggga atatacattc ggacagggca
     4561 atattgccct tgagaatgcg ggcataatcc tgtttgaatt cctcgggagt ggctccccgg
     4621 tagagtgaaa aagtccgggt aatgtcggta gtgccctcga ggtattggcc tccggtgtct
     4681 accaataggt aaccgtcggg caaaattctg gctttggtac tctccgaagg actgtagtga
     4741 acaatggctc cgtgttcgtt gtaactaatg atggaataaa aactgtctcc tttgcagtga
     4801 tcctgttcca gtctgaattc gctcattttg cggaccaggt cggtctcgta gagctctttt
     4861 ccttgttcca gggcagcttc gaaccagcgc cagaaacgaa ccaatgccac accctctttt
     4921 tgcatggcgg tacgaaatcc ggcaatttcg accggatttt tgattgattt cagagctgtt
     4981 gtgggagcag taccttccac ccggcggcaa tttgagggta tatgtttgta aatgaaatag
     5041 ttgatacgtg tcgtgtctgt aaagactccg gaacctgccg gaagtttact taaatctgca
     5101 tacagagccg aatagtcacg tagttgaata ttgttttctt tgagataggc cagcgtgctt
     5161 tcgtctattt tgttcttgtc ggtgtatagg ttcgcgatgt ccgggccgat cagggcaaaa
     5221 gcaagaccta ccggattgaa atccacgtca ctgcccctta ggttaaacag ccaggcgata
     5281 tcgtccagac cgctgacggg cagatagtct gctccctgac aactgagctc ggctctgagt
     5341 tccgaaagtt tttcttttac gctcttgccg gcatagccga cggggaaaac ataaaaaggg
     5401 gaatccggta aatccggcct gtcgggccat acccgggtgt aaggtttgaa atcgtcgacc
     5461 aatcttaccc cgtgtttatt gaagacctgg atcattttga gcacttcgcg agcggcgaat
     5521 acttctccgt cgataccggc cgaacgataa ttcatttcgg ctatccattc gggaatgccg
     5581 ggagtttcgg gcagggtggt tttaaacaaa tcgataccac tgcccctgag ttcctgttcc
     5641 gcctgaaggt aatacctgga gtctgtccat aagccggctt tgtccttgcc tactaccaga
     5701 gttccggcac tacccgtaaa ccccgagagg tattttctga atttaaaatg cgaggaaacg
     5761 tattctccct gatggctgtc tgaggttgga attatgcagg cttctatggc attggtctcc
     5821 atcagatttc gtaatgccgc caatcttttc ggaatgctgt tgtacattat attaaggtat
     5881 atggtcggtt tagtaaaagt gcaaaggtag taaaaaaaac agctttttaa cttccggctc
     5941 ttaattgaat tttgggtagt aaacataaaa tattttcgaa agattctgag ttttttttgt
     6001 ttactgattt gttttgtcta cttttgcagg ccgaattaaa atcattttat acaaaacacg
     6061 attatgatca ttgttcccgt caaagagggt gaaaacatcg aaagggcttt gaagaagttc
     6121 aaacgtaaat tcgaaaaaac aggaaccgtt aaagaactaa gaaacagaca agctttcaga
     6181 aagccttcgg ttgtcaaacg ccagcaaaaa atgaaagctg tttacgttca acagttgcaa
     6241 caaaacgaag agtaaaacag acagaaaaac acttaaaatt aggtctgttt ttttgttgtt
     6301 ctgaaatagc tttgctatat tcgttgcgga gcaatttttc aagcagcgcc tgtgtgggta
     6361 gagcaataca tacaatactt acggtatgag aaaaattatt cttctcatac tgtttttgcc
     6421 tataaacgcg atttggaaca attccttgcc tttgcttccg acacttttca actggaacat
     6481 ccctcagaca tcgacgccga tattatcaga gcatggatgc ttgccttaat ggagaagggc
     6541 gacactgccc gttccgtgaa tcgcaaactt tcttctctcc ggtcgtattg gcgttatctg
     6601 ttgcgtcagg gtttagtgac atccaatccc acacaaaaag tgctgacacc caaaattaaa
     6661 aaggctcttc cttctttttt aaaagaggag gaattaaacg acttactcga taaagccagg
     6721 gaggaaggac agactgattc ctttaccgaa agccgtgata atctgatttt agagatgttt
     6781 tgccttacag gtatccggcg ggcggaatta attggtctta aaaccggaga cgtagacata
     6841 ggctcagctt cgctcaaggt tacgggaaag cgcaataaac agcgtatttt gccctttggg
     6901 accgctctaa aaaagtcaat agaaaattat ttggtgttac gcagcgatta tctggataaa
     6961 ctcgaagatt acccggaagt ggagccgccc ttttttatca atgccaaagg acgggctctg
     7021 aatcctcgcc gggtatatag tcttgtcaat aaaaaattag cagaggtggg caatttgtct
     7081 caaaaaagcc cgcatgtgct caggcataca ttcgccacca ccttattaaa caggggagcc
     7141 gaactgaatg cagtcaagga actcctgggg catgccagct tgtctgctac ggaagtgtat
     7201 acccatacta ctttcgaaga attgaaaaaa gtgtataaac aagcccatcc aagggcagaa
     7261 aattgaaaag gagaataatt atggaaatta gaattcaatc cgtgcgtttt gatgcgacca
     7321 gtgctttaga gaaatttata caaaaaaagg tggctaagct ggctcagtac tacgaaggta
     7381 tcttgtcggc cgaggtaact ctgaaagtaa ctaaacccga aactgtcgca aacaaagaag
     7441 ccttgatcag gcttaatatt tcaggccggg atttgttcgc ttccaaaacc tgtgacactt
     7501 ttgaggaatc tatcgacgaa gctgtggaag ctttggagaa acaattgaag agacataaag
     7561 ataaaatccg gagtaaataa aatttcaaaa attgttttgt ggttaaaaaa tattgcctac
     7621 atttgcagcc gtttctctaa aagggagttt tagagcatgg ttgcttcgac gtaatcgtgt
     7681 aaattgaagg aattggggca gttaccagag tggccaaatg gggcagactg taaatctgct
     7741 ggttgatacc tacggtggtt cgaatccatc actgcccaca aattattttt aaggggatcg
     7801 gttttttgga attattgaac ccaaaaatat tttgcgggaa tagctcagtt gatagagcat
     7861 tagccttcca agctgagggt cgcgggtttg agtcccgttt cccgctcttg cctgtgtagc
     7921 tcagtggtag agcacttcct tggtaaggaa gaggtcccga gttcaaatct cggcaccggc
     7981 tcaaacagta tagatagatt acttacttat tttaattttc gattatggca aaagaacatt
     8041 ttaacaggac taaaccccac gtcaacatcg gtacgattgg tcacgtggat catggtaaaa
     8101 ccacattgac tgccgctatc actacgattt tggcaaaaag aggactttcc gaagtaaaat
     8161 ctttcgatca gattgacaac gcaccggaag aaaaggaaag aggtattaca attaatacat
     8221 cgcacgttga atatgaaacc gaaaatcgtc actacgcaca cgtagactgc ccgggacacg
     8281 ccgactatgt taagaacatg gttaccggtg ctgctcagat ggacggtgcc attatagttg
     8341 tagccgccac cgatggtcct atgccccaaa ctcgcgaaca tatcctcctc gcccgtcagg
     8401 taaacgttcc cagactggtt gttttcatga acaagtgtga tatggttgat gacgaagaaa
     8461 tgctcgaatt ggttgaaatg gaaatgcgcg atttgttggg tttctatgaa ttcgatgccg
     8521 aaaatacacc tgtaattcgc ggttctgcac tgggtgcttt gaacggtgtt gcccaatggg
     8581 aagacaaagt aattgaactt atggctgccg tagacaattg gattcaagtt cctccccgtg
     8641 ccgttgacaa acctttcctt atgcctgttg aagacgtatt ctcgatcaca ggtcgtggta
     8701 ccgtagctac cggacgtatc gaaaccggta tcatcaaaac cggtgaagaa gtacagatta
     8761 tcggtttggg tgccaagggc aagaagtctg ttgttacagg tgtggaaatg ttccgcaaga
     8821 tcctggatca gggagaagcc ggtgacaacg taggtttgtt gctcagaggt attgacaagg
     8881 atgaaatcaa acgtggtatg gttgttaccc accccggaaa ggttactcct cacaccagct
     8941 tcaaagcttc ggtctatatc ctgaaaaaag aagaaggtgg acgtcacact cctttccaca
     9001 acaaataccg tcctcaattc tacattcgta cccttgacgt aaccggcgaa attacccttc
     9061 ccgaaggaac cgaaatggtt atgcccggcg acaacttgga aatcaaagta gaattgatct
     9121 atccggtggc ttgcagcgaa ggtttgcgtt ttgctatccg tgaaggcgga cgtaccgttg
     9181 gatcaggaca gattactgaa attctgaaat aattaaggca aatatactct cggtggggct
     9241 gtcagcgtga tagctctacc gaggggtgtt taatacgggt ctagctcagt tggtagagca
     9301 tcggtctcca aaaccgagtg tcgggagttc gagtctctcg acccgtgcca taaaccccat
     9361 cctatgaaaa agttttttaa aagggtcgcc acctatgtca aggatgcata caatgaactc
     9421 gtacacaagg tctcttggcc tacaccgtct gagttgagca atagtgcgat tgtcgtgtta
     9481 actgcttccc tggtgattgc acttattgta tttatcattg atttaagctt ggaaaatctc
     9541 atgaccttca tctatgagaa ggtgttttaa gctggaagaa aaatgtctga aacgtcaaag
     9601 aaatggtatg ttttacgtgc cattagcggt aaagaaaaca aagtgaagga gtatattgaa
     9661 gcttccatca ataattcttc tcttggtgag cacgtgtctc aggtattgat tccaacagaa
     9721 aaagtttacc agtcacgtgg cgggaaaaag gtgctcaaag agcgttctta tttgcccggt
     9781 tatgtgctgg tcgaagccaa tctggtaggt gaagtggcac acgcccttcg aaatgttccc
     9841 aatgtgctcg gttttttggg tggaatgaac aacccccagc cgctccgtga atccgaaata
     9901 aatcgcatcc ttggtaaggt cgatgaatta caggaagccg gagacgacat cagtatcaat
     9961 tactatgtcg gtgagaacgt aaaggtgaat tttggaccct ttagcggttt tagcgcaacc
    10021 atcgaagagg tgaacaatga gaaaaagaaa ctgaaagtca tggtgaaaat tttcggccgg
    10081 aaaactccgc tcgaattggg ttttatgcaa gtagaaaaag aataaaagat ggttacgcat
    10141 cctgtatttt ttttcaaaac aatcctcaaa ttttaaatta aaacgaaaat ggctaaagaa
    10201 attgccggtc aaatcaaatt gcaaataaga gggggcgcgg caaatccctc tccgcctgta
    10261 gggcccgcac tgggttccaa gggtgtgaac ataatggagt tttgcaagca attcaatgcc
    10321 agaacacagg ataaagccgg aaaggtgttg cctgttgtta ttacgtacta ttccgataag
    10381 tctttcgatt ttgtcgttaa aactcctcct gcagccgtac aactgttgga ggcttccaaa
    10441 accaaattgg gttcggctga gccaaatcgc aagaaagtgg gcgaagtcac ccttgatcag
    10501 gttagggcaa ttgcagaaga caaaatgagt gacttaaatt gctttacgct ggaatcggct
    10561 atgaaaatgg ttgccggtac agccagaagc atgggtatca gaataaaggg tgaattaccc
    10621 acaaacaatt aaacttcgac gaaaatggca aaattgacaa aaaatcaaaa attggctttg
    10681 gagaagatgg aagtgggtaa agcgtattca ttgagagaag cctcagaact ggttaaggaa
    10741 atcaccttta ccaaatttga cgcttctgtc gatatagacg ttcgcctggg agttgatccc
    10801 cgtaaagcca atcaaatggt acgcggcgta gtgtcgctgc ctcacggaac cggaaaacaa
    10861 gtacgggtat tggtgttgtg tactcctgat aaagaagccg aggctaaaga agccggagcc
    10921 gatcatgtag gtcttgacga ttacatcgaa aagataaaag ccggttggac ggatgtggat
    10981 gtaattatca ctatgccttc gaccatgggt aaaattggtg ctttaggccg aattttgggt
    11041 cccaggggct tgatgcccaa ccccaagagt ggtactgtta ccatggaagt cggcaatgca
    11101 gtcagagaag ttaaacaagg aaaaatagac tttaaggtag acaagaacgg tattgttcat
    11161 gctgccatcg gaaaagtcag ttttactccg gatcaaatcc gtgagaatgc caaagaattt
    11221 attctcactt tgatcaagtt gaagccgact gctgccaaag gcacttattt gaagagtatt
    11281 tatctttcaa gcacaatgag caagggcatc aaagtggatc ccaagagcat tgaagattaa
    11341 aaaacagaac aattatgaaa aaggaagata aatatattgt agtagagcaa atcactcaat
    11401 tggtaaacga gtacgaacat ttttatgtta ccgatatcgc atccttgaat tccgaagaaa
    11461 cctcttcgct tagaagggct tgtcataagg aagatgttaa gctctacgta gttaaaaaca
    11521 cactgcttaa aaaagcgctg caaggtttgg aaacagacta ttcttctctt tttgatacct
    11581 taaaaggcaa taccgctctg atgttttgca acaccggaaa tgcggcagcc aaattgatta
    11641 aggaatttag aaaggacaaa agtgttcctg ctttgaaagc tgcatacgta gaagagtgtt
    11701 tctatatcgg tcccgaacat ttggacaccc tgattcatat caagagcaaa aatgaactga
    11761 ttgcagatat tgttgccttg ttacaatcac cggccaagaa tgtggtttct gctctccaaa
    11821 gcggaggtac aatacttcac ggtgttttga aaacactgga agaacgcgaa aattaggtct
    11881 tctgataaaa agaagtctca aaaacaaacg aataaacaat tttaaataac aattaataca
    11941 ttactaaaat ggcagatttg aaagcttttg ctgaacaatt ggtaaattta accgtcaaag
    12001 aagttaacga acttgctgag atcctcaaga atgaatatgg tatcgaaccc gctgctgctg
    12061 ctgttgccgt ggctgccggt cctgctgctg ccggtcctgc tgcagaagaa aaaacttctt
    12121 tcgatgtggt tttgaaatca cccggagcag ctaaactggc tgttgtaaag gcagtaaaag
    12181 aatcttgtgg tcttggtttg aaagaagcta aagacttggt agatgccgct ccttccgttt
    12241 tgaaagaagg cgttacaaaa gacgaggcag atgcgctgaa gaaaatgctt gaagaggcag
    12301 gtgccgagat tgaacttaaa taaaagtact tgcctgatca acaggtcccc cggtttagaa
    12361 tctccgtgat tggaggttct aaaccttttg tgtctatatt cttttttaag ttcttataat
    12421 ccctcgataa atgtcctcaa ctaccgacaa ccaaagaata aattttgcat ccataaagag
    12481 cccgatcgat tatccggatt tcttagaagt acagttgaag tcatttcagg acttcctgca
    12541 attggatacc ccacccgaaa aaagaaaaaa cgaaggattg tacaaggtgt tttccgaaag
    12601 ttttccaatc acggatacac ggaacaattt tgtcctcgaa tttatcgatt actacgttga
    12661 tcctccccgt tataccatcg aagaatgtat tgaacgggga ttgacttaca gtgttcctct
    12721 aaaggcaaag ctcaaacttt attgtaccga tccggatcac gaagattttg aaaccaaatt
    12781 tcaggatgta tacctgggtg cgtttcccta catgaccccc aaaggcactt ttgttgttaa
    12841 tggtgccgag cgtgtaattg tatcgcagtt acatcgttca cccggggtat tttttggtca
    12901 aagcgtgcat gccaacggga cgaaacttta ttccgcccgc attattccct tcaaaggctc
    12961 gtggatagaa ttcgccacag acatcaacaa cgtgatgtat gcctacatcg accgtaagaa
    13021 gaaattaccc gtaactactc tgttgcgtgc catcggtttt gaaacagaca aagatattct
    13081 ggaaattttc aacctggccg aagaaatcaa agtaaccaag accggtttaa aaaaacacgt
    13141 cggccgtaaa ctggctgccc gtgttctgaa aagctgggtg gaagactttg tggatgaaga
    13201 caccggcgaa gtggtttcca tagaaaggaa cgaaataatc attgacaggg aaaccgttct
    13261 tgaaagcagt catattgacg acattcttga atctggaaca cctactgtcc ttctgcacaa
    13321 agaggatccc gatatgtccg atttcagcat catatataat acgctgcaaa aggatccgag
    13381 taattcagag aaggaagccg tgctttatat ttaccgtcaa ttgcgtaatg cagaaccggc
    13441 cgacgattcc agtgcccgcg aagtaatcca gaatttgttc ttctccgaaa aacgttacga
    13501 cctcggagaa gtgggacgtt accggattaa taagaaactc cagttgaata cggatatgga
    13561 taccagggtc ctgaccaaag acgatattat tcatatcatc cgttatttaa tcgaactggt
    13621 caattccaac gccattgtgg acgatataga ccacttgagc aacagacgtg tgcgcaccgt
    13681 cggcgagcaa ctcaacaatc agtttggcgt gggccttgcc cgcatggcaa gaaccattcg
    13741 cgacagaatg aatgtacgcg acaacgaagt gtttgctccg gttgatctaa tcaacgccaa
    13801 aacggtatca tcggttatta attccttctt tgggaccaat gccttgtcgc agtttatgga
    13861 tcaaaccaac cccctggccg aaatgaccca taaacgccgc ttgtcggcct tgggtccggg
    13921 cggtttatcg cgtgaacgtg ccggttttga agtccgcgac gtgcattaca cccactacgg
    13981 acgtctttgc cccatcgaaa cacccgaagg tcccaacatt ggtttgattt cgtctttgtg
    14041 tgtgtttgca aaaatcaata aacttggttt tatcgaaact ccctaccgca aagttagcaa
    14101 cggtatagtc gaaaaagagg tggttttctt aaccgcagaa gaggaagaaa atctggtagt
    14161 ggcacaaggt aatgctctct tgaatgaaga cggttctttc gttcgcaaca gaattaaagc
    14221 tcgtctcgaa gccgacttcc cggtagttga acccaataag gttgacctga tggacgtttc
    14281 tccttctcaa atagcctcta ttgcggcctc tttgattcct ttcctggaac acgacgatgc
    14341 caaccgcgct ctgatgggtt cgaacatgat gcgtcaggcc gtgcccttga tacgttgcga
    14401 ggcaccgata gtaggtaccg gtctggaaaa aactctgata aaggattccc gtactcaatt
    14461 gatggccgaa ggcgatggag tggtcgaata tgtggatgct acaagcattc atattcgtta
    14521 cgaccgtacc gaagacgaag aatttgtgag tttcgacgac gctgtcaaaa tctacaacgt
    14581 tcctaaattc caaaaaacga accaaagcac caccatcgac ctgaaaccca ttgttcgtaa
    14641 gggacaaaga gtgactaaag atcagctttt gaccgaaggc ttcgctaccg aaaaaggcga
    14701 attggctctt ggccgcaacc ttaaagtggc ttttatgccc tggaaaggct ataacttcga
    14761 agacgccatc gtgttgaacg aacgcttggt acgcgaagat atttttactt ctgtgcatgt
    14821 cgatgagtac actctcgaag ttcgcgaaac caagcgcggc atggaagaat tgactgcaga
    14881 tattcccaac gtcagcgaag acgccaccaa agacttggac aaaaacggta tcattcgtat
    14941 cggtgctcat gtcaagcccg gtgacattct tatcggaaaa attactccca agggagaatc
    15001 cgatccttca ccggaagaaa aactcttaag agctattttt ggagataaag ccggtgatgt
    15061 taaggatgcc tctctgaagg caactccttc tctcagtggt gtagttatcg acaaacgtct
    15121 cttctccaaa gccattaaga accgcaagac ccgcttgacc gacaaagcca ttctgcctaa
    15181 acttgatgaa gaattcgaag aagaagtagc cgccatacgt gccgtattgc tcgaaaaact
    15241 ctgggttttg gtaagcggta aaacttctca gggaatcaaa gataatctgg ataccgaagt
    15301 catacctaaa ggcgttaagt tcactcaaaa gatcctgaat gaaatcaact attcttccat
    15361 tcagttgagt aaatggacct cagacaatca caagaacgat atgattcgcg atctgattat
    15421 caattacctc aagaaggaaa aagaattgga tgcgaaactc aaacgtaaaa agttcaatgt
    15481 gaccataggc gacgagttac ccgccggcat cattcagatg gccaaggttt acgttgccaa
    15541 aaaacgcaaa atcaaggttg gtgacaagat ggccggacgt cacggaaaca aaggtatcgt
    15601 ttcgcgcatt gttcgccagg aagatatgcc ttttatggaa gacggaactc cggttgatat
    15661 agtcttgaac cctcttggcg taccttcgcg tatgaacctg gggcaaattt ttgagacggt
    15721 attgggatgg gccggacaaa agttaggcgt taaatttgcc acgcctattt ttgacggtgc
    15781 cgagttggaa gatctggaac aatggtccga caaagccgga ttaccgcgtt acggtaaatg
    15841 ctatctctac gatggcggca ccggtgatcg ttttgaccaa ccggccacgg tgggtgtaat
    15901 atatatgctt aaactcggcc acatggttga tgataagatg catgcccgtt ccattggacc
    15961 atactccctg attacccagc aacctctggg tggtaaggct cagtttggcg gccagcgttt
    16021 cggagaaatg gaagtttggg cgctcgaagc ctttggagct tcgcacatac tgcaggaaat
    16081 cctgaccatt aagtcggacg atgtagccgg ccgcagcaaa gcttatgaag caatagtcaa
    16141 gggagaacct atgccgcagc ccggtattcc cgaatccctg aatgtattgt tgcacgaact
    16201 cagaggcttg ggcctaagca tcgaattaga ttaacaactt caattacaca cagaatatgg
    16261 ctctcaaaaa agatataaag accaaaacaa acttctcgaa aatctctata ggattggctt
    16321 cgcccgaaga aattcttgaa ttgtcgagtg gtgaagtatt gaagccggaa accattaatt
    16381 atcgtaccta taaacccgaa cgtgacggtt tgttttgcga aaggattttt ggtcccgtaa
    16441 aagattatga atgtcattgc ggtaagtaca agcgtatccg ttacaagggc atagtttgcg
    16501 accgttgcgg ggtagaagtt acagaaaaga aagtacgtcg tgaacgtatg ggacacatac
    16561 agctggtagt cccggttgtt cacatttggt atttccgttc actacccaac aaaattggct
    16621 atttgctggg tttacccacc aaaaagctgg attcggttat ctaccacgaa cgttatatag
    16681 ttatccagcc cgggatactc gaagaggaag aaggtgtcaa ggttaacgac ttattatcgg
    16741 aagaagaata tctggctctc atggataaac tgcccagaga aaatcaaatg ctcgaggaca
    16801 gcgatccgca gaaatttatt gccaaaatgg gtgccgatgc agttcacgac cttttatcca
    16861 gactcgatct ggatgcattg tcgtttgatt tgcgtcaccg tgccgacaca gacacctcca
    16921 tgcagcgtaa gaacgatgcg ctcaaacgtc tgcaggtggt ggaagctttc cgtgcagcca
    16981 gacacagaaa caaacccgag tggatggtca ttaagattgt cccggtaatc cccccagagt
    17041 tgcgtcccct ggttcctttg gacggtggtc gttttgcgac ttccgacctg aacgatctct
    17101 accgtcgtgt tatcatacgc aataatcgtc tgaaacggct tatagaaatt aaagctccgg
    17161 aggtgatatt aaggaatgaa aaacgcatgc tgcaggaagc tgtcgattct ttgtttgaca
    17221 actcccgcaa atcaagcgcc ataaaaaccg attccaaccg tccgcttaag tctttgtccg
    17281 attcgctcaa gggtaaacaa ggccgtttcc gtcagaatct gttgggaaaa cgtgtcgact
    17341 actcagcccg ttctgttatt gtagtcggtc ctgaattgaa aatgcacgaa tgcggattac
    17401 ccaaggatat ggctgcagaa ttatataaac cgtttatcat acgtaaactg attgagcgtg
    17461 gaatagtaaa aaccgtaaaa gccgccaaaa aatttgtaga ccgcaaggat ccggtggttt
    17521 gggacatcct ggaacacgtc atgaaaggcc atcccgtact gcttaaccgt gcccctactt
    17581 tgcatcgtct cggtattcag gcattccagc cgaaaatgat agaaggcaaa gcaattcagt
    17641 tacatcctct tgcttgtacg gcttttaatg ccgacttcga cggagaccag atggctgtgc
    17701 atcttccctt gggtaacgaa gctattgccg aagctcaatt gttgatgttg tcttcgcaca
    17761 acattctgaa tccggccaat ggagctccca ttaccgttcc gtcgcaggat atggtgttgg
    17821 gtttgtatta tatgaccaaa gagcgaatag gggctcaggg agaaggattg actttctatt
    17881 ctcccgaaga agccaaaata gcctacaacg agaaaaaggt ttctttgcat gccgttgtaa
    17941 aagtgcgtat tgaaggtgaa ctgatacaga ctaccatagg acgtattctc gtaaacgaat
    18001 atgtgcccga agaagttggt tttgtaaata ccgtcctttc caaaaaagaa ctgcggaacg
    18061 taataggtaa agtcattgct tcttgcggag tagcacgtac agctcagttc cttgatgata
    18121 tcaaggattt aggttactct atggctttcc agggaggttt gtccttcaac ctggacgatg
    18181 tgatcgttcc ggacgaaaaa accgagttaa ttcagggagg ctatgaagaa gtcgaagcca
    18241 tcatggaaga atggagacag ggttttatca ctcccaatga acgttacaat aagattatcg
    18301 acgtttggac cattgtaaac aacaaacttt ctaatattct catcaagcag ttggccgaag
    18361 acgatcaggg tttcaattcc gtacacatga tgatggattc cggagctcgc ggatcgaaag
    18421 aacagattcg ccagttgtcc ggtatgcgcg gcttaatgaa taaacctcaa aaatttggcg
    18481 ctgagggtgg acaaattatc gaaaacccca ttttggccaa ctttaaagag ggtttgtcgg
    18541 tgctggagta ttttatttct actcacgggg ctcgcaagtc tttgtctgat actgcactaa
    18601 aaacagctga tgccggatat cttacacgtc gtttggttga tgtatctcac gatgtcattg
    18661 tcaacgaaga agattgtgga accctgagag ggcttacagc cttggaaatc aaaggggtag
    18721 catcacttta tgaacgcatt ctcggacgcg tttccgtaca cgatgttttc aacccggata
    18781 ccaacgagct tttggtgtca gccggccagg aaatcacgga agacattgca cagaaaattc
    18841 aggactctct cgtcgaccgg gtggaaatcc gttcggtatt gacctgcgaa tccaaaaaag
    18901 gagtttgcgc caaatgttat ggccgtaatt tagccaccgg acgtatggtg gaaaaagggg
    18961 aggctgtggg cgttattgct gcacaatcca ttggtgaacc gggaactcag ttgacactgc
    19021 gtactttcca tgccggtggt gttgccaaga gcgaaaatgt tgaaaacaaa cttgtagcac
    19081 cttacgacgc cctggttgaa attgatgaat tacgggtagt accctctttt gaagacaaaa
    19141 acgtcaggat cgtagtaggc cgttatgctg aaatccacct gatcgaccct gtcaccaata
    19201 tggttaccac acataatctt tcttacggat cacgtttata cgttgatccc ggggatatgg
    19261 ttaaacaagg cgatctgata tgtgaatggg atgtctataa tgcggtaatc atctgcgaac
    19321 aatcgggtaa aatcgaattc gaaagcattg aagaaggtgt cacttatcag acttatatgg
    19381 atgagaccac caagattaag gataagatca tcatcgaatc caaagatcgt tcccatgtgc
    19441 ccaccatcca tattatggac gataagggtc aacgcctgta ctcttataac ctgcccgtag
    19501 gcgctcacct gctcaaagaa tcgggcgacc gtatcgaagc aggccaggtg ttggtcaaga
    19561 tcccgcgtgc cattcccaaa tcgggcgaca tcaccggagg tcttccccgt gttaccgaat
    19621 tgttcgaggc ccgcaaccct tcaaatcctg ccgttgtttc cgaaatcgac ggagatgtaa
    19681 tagaattggg acgtatcaaa cgtagtaatc gtgagattgt tatccagtca aagaccggtg
    19741 agatcaaaaa atacctggtt cccttgtcca aacaggtgct ggtgcagctc aatgactttg
    19801 ttcgtgccgg aactcctctg tcggaaggat ctattacgcc tgcagacatc ttggccatta
    19861 aaggtccgac tgctgttcag gaatacattg taaacgaagt taagaatgta tatcgcatgc
    19921 aggaagtaaa gatcaacgac aaacatttcg aggttattgt gcgtcagatg atgcgcaagg
    19981 tagagattgt cgatgccggc gatacccatt tcctagaaca acaagaggtc gacaaaaatg
    20041 aatttatgga agaaaacgat cgtatctggg gtaaaaaagt agtgctcgat ccgggtgatt
    20101 ctgccacttt gaaagccggt atgatagtta ctgcccgtaa attgcgcgac gagaattcca
    20161 gtctgaaacg tcgcgacttg agactggtag aagtaagaga cgccattcct gccacttcca
    20221 atcagatttt gcagggtata acccgtgcag ctcttcaaac cagcagcttt atgtcggcag
    20281 cttccttcca ggaaaccacc aaggtactca acgaagcagc tatacaggca aaagttgacc
    20341 gcctggaggg catgaaagaa aacgtaatta cgggtaacct gataccggcc ggaaccggac
    20401 aacgtgaatt tgaaaagctg atagtcggtt caagagaaga atttgagcgt atcgaagcaa
    20461 cccgcgacag cctcactcag gacgttgtgg ttgaagaagc cgaacatgaa taactgatta
    20521 gtaagtaatc tttagcataa tcggaaagag gaaggccgtt ccaaagaacg gccttcctcg
    20581 tgtttacccg gtacgaacat tctctaagtg gtaaaacttt ttccctcaac tgtcggatta
    20641 aattaaaaat tcatctggtt cttattaaaa atataccatt aatcccaccg ataattggct
    20701 aaaatttggt cctttttgag attgaaaaat gctgtttggt gtgt
//
LOCUS       DAOSTD010000027        19981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_2879_length_19981_cov_15.937970, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000027 DAOSTD010000000
VERSION     DAOSTD010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19981
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2879_length_19981_cov_15.937970"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..1587)
                     /gene="mnmE"
                     /locus_tag="PLI37_03100"
     CDS             complement(193..1587)
                     /gene="mnmE"
                     /locus_tag="PLI37_03100"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOQ58036.1"
                     /translation="MKQDTICAISTPPGSGGIAVIRLSGPKAIAIADQLFVPGKNGCP
                     VREQASHSCQYGQLMEEADKVLDYVLLSVFRGPNSFTGEDTVEFYCHGSLFIQQRLIQ
                     LLIQLGCRQAGPGEFTQRAFLNGKMDLSQAEAVADLIAADSSSANQLALKQMRGGFSK
                     DLADLREKLLHFVSLIELELDFSEEDLIFASRDELKKLADQLESKLSALCESFSLGNA
                     IKNGIPVAIVGETNSGKSTLLNKLLNEDKAIVSDIHGTTRDSIEDLVSIRGLSFRFID
                     TAGLRKTKDRIESLGIERTYQKIEQASIILWLIDSTQLSEHIDWMAERIIPRSQGKKL
                     LLVFNKTDKIAGEELGIVENLFAHIPGDRIQISAKYGQNIEELKSKLIELTNLPEFDK
                     TDTIVSNIRHFEALSAGLEAIRRVREGLQQNLSGDFIAQDIRECMYHLGLITGQISND
                     EVLGQIFSRFCIGK"
     gene            complement(1593..2465)
                     /locus_tag="PLI37_03105"
     CDS             complement(1593..2465)
                     /locus_tag="PLI37_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOQ58037.1"
                     /translation="MSLHFLESELIINEDGSIFHLRLKPEQLADRVVLVGDPDRVSLV
                     ASKFDIVECRVRNREFHSVTGSYYGKRMTVLSTGIGTDNIDIVMNELDALANIDFEKR
                     TEKAEKKSLSIVRIGTTGGLQEDLPVGSFLISEKSIGLDGLLNFYAGRDKVSDLKFEE
                     AFCNHTAYLKQWARPYVVSANPELLARIGQKDMLRGVTVTANGFYGPQERQLRLKLAL
                     PGFNESLRSFRYDSYRITNYEMESAGVAGLAALMGHKAMTVCLVIVNRFASDAKTNYK
                     DRMSELITLVLDRL"
     gene            complement(2521..3495)
                     /gene="floA"
                     /locus_tag="PLI37_03110"
     CDS             complement(2521..3495)
                     /gene="floA"
                     /locus_tag="PLI37_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861713.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOQ58038.1"
                     /translation="MSPGFGLLILIPVIILLIIFFYYVPFFLWLSAKVSGVNISLIQL
                     FLMRIRKVPPTIIVNALIEGHKAGLKELRRDDLEAHYLAGGHVQQVVHALVSASKANI
                     DLSFQMATAIDLAGRDVFTAVQMSVNPKVIDTPPVTAVAKDGIQLIAKARVTVRANIK
                     QLVGGAGEDTVLARVGEGIVSSIGSSENHKMVLENPDSISKVVLRKGLDAGTAFEILS
                     IDIADIDVGKNIGAELQMDQAQADKNIAQAKAEERRAMAVALEQEMKAKAQEARARVI
                     EAEAEVPKAIAEAFRSGNLGIMDYYRMRNIESDTNMRESIAKPPTPKK"
     gene            complement(3535..4014)
                     /locus_tag="PLI37_03115"
     CDS             complement(3535..4014)
                     /locus_tag="PLI37_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOQ58039.1"
                     /translation="MNIALVVGLIVLGIFFMILEIFFLPGVSVALIGGLACFAGGIIV
                     AYTNLGPTAGHITLISSAAAMAIAFVWFIRSKTLKKMSLETNIDSKIAAHQGAEVKVG
                     DVGRCISRLAPTGKILINGKTLEGRSENEILDEGTQVLVIRISDLNVIVKKYDDNVS"
     gene            complement(4021..5460)
                     /locus_tag="PLI37_03120"
     CDS             complement(4021..5460)
                     /locus_tag="PLI37_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ58040.1"
                     /translation="MRNLLIVFLLFTVPLSAQTLTEARAMYINGEYEKALPVFEQELK
                     RKPKDVNLNYWYGACLYHTGNPTAALPYLVNAEKNKIPTASLLLADYYHRQYQFDKSA
                     GHLDKYIAFGLSSNSDKVARMLKASEKAKQMLKAVEDVVFIDSLVVPMDSVFENIRLH
                     SANGTVLPLRKLFPEKGDSLSVAYLTERKDCVFYDDTTAENGWDIFSLNRTMNSWETI
                     DLVKSLNQASNERFPYVLNDGITIYFASDGYNSIGGYDLYVSRYHLASESFFTPEPLP
                     MPFNSVYNDYFYIVDEYVGRGFLVTDRYAGKDSVTIYTFIPESTRKILSGKSEQEIIE
                     YARLTSISLTQDGIDTDSIQTMLEERYQYDNNTVLHELEHTGSEQLRFFVRDDLVYEN
                     ENDFRSEEARQAYLQYLKLQARFNSLNTQLEADRPKYTGKDNPEQQELASRILNAEWV
                     LLFLERDIPLKEYEIRRLELEKIDAPINN"
     gene            complement(5649..8570)
                     /locus_tag="PLI37_03125"
     CDS             complement(5649..8570)
                     /locus_tag="PLI37_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HOQ58041.1"
                     /translation="MNTRRKTAALFFAAVLIAGTTLAANPARIKFNFNPSWLLHIGDQ
                     PGAHNPEFDDTEWKTVHLPAAFNEDEAFAKSIHDLTDTVVWYRKHFKIPSTYKNQKVF
                     IEFEGLRQAAEIWINGREIGLHENGVMAFGLDLTDHLVFGNKENVIALRIDNDWKYKE
                     KATGTGFQWNNNNFNANYGGLTKNVVLHICGKLYQTLPLYSHLGTVGTYVYATDINVR
                     DKTAVLNVESEVKNEQEKAQTFYMKVIVRDNDHNMIREFNSGEITLQPGESRIVKASG
                     NLKALRFWSWGYGYLYNVTSSLISGKKTLDLVRTQTGIRKTAFRNGMFYLNERVLHLK
                     GYAQRSSNEWPAVGMSVPPWLSDFSNGLMERGNANLVRWMHVTPWKQDIESCDRVGLL
                     QAMPAGDAESDVEGRSWELRKELMRDAIIYNRNHPSIIFYECGNETISEEHMAEMKVI
                     RDIYDPNGGRAIGSREMLDSKEAEYGGEMLYINKSAGIPMFAHEYCRDEGLRKYWDNY
                     SYPYHRDGEGPLYRNEDASAYNRNQDSFAEEAVRRWHEFWVHRPGTGRRVSAGGVNII
                     FSDTNTHFRGAENYRRSGEVDAMRIEKEAYYAHKIMWDGWVEVEGNKYSNHIIGHWNY
                     EPGTVKDVLVVSNASAVELFLNGRNLGFGERQHHFLFKFKDVPFEAGSLKALSYDSRG
                     QLAGEPAEIKTADEAAALRLQYWQHPKGFRADGSDMVLIQVEVVDKNGLRCPLANHLI
                     HFDVEGPAEYRGGIAQGENNYILSRDLPVECGVNRILLRSTTEAGKIVVRANAEGLSV
                     DSLVLNSLEVPVKDGLSTFFPKDELISYLWRGATPGYASFVMSRLPLSIQSAVAGSNP
                     EEAALSFDDNELSRWDARGPAGEVWIDYTLSRSAVLTEACLKLGNFRNTSYHLRILTE
                     QNEVLWEGQTEKTLGYVNLSLNPASPTRNVRIESLGDGDQARLSIVEAEFYTQIPAQG
                     R"
     gene            8722..10500
                     /locus_tag="PLI37_03130"
     CDS             8722..10500
                     /locus_tag="PLI37_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOQ58042.1"
                     /translation="MSEKDWYIKMILWREKHIKESNFILILSFVVGLLTSLAALLLKT
                     LIHQIHTWVSSGSAIDHANYYYLLYPALGIFLTALFVRYVVRDDIGHGVTKILYAISR
                     RQGRIKRHNTWTSMVSSSITIGFGGSVGAEAPIVLTGAAIGSNLGSFFKMDHKTLMIL
                     IGCGAAGTIAGIFKAPIAGLLFAIEVLMIDFTVASVMPMLVSSVTAATLAYFMSGTGV
                     AISFDGADVFQLVNVHWVIVLGIFCGLVSLYVIRGMNTIEGWYKKIKNPWLKIILGAL
                     ALGLLIFLFPPLYGEGYESIEALINGNLTELTHNSLFYGVQQHFGWLALYLLLIIVFK
                     VFASASTNGAGGVGGVFAPSLFVGSFTGFLVATAINYFGLGTVSPGNFALMGMAGLMS
                     GVMHAPLTGMFLIAELTGGYDLFLPLMIVASVSFMTILLFEKHSIYSMRLAQKGQLLT
                     HHKDKAVLTLMRIENLIEKDFDVLSPDMTLGDLVKVISKAKRNMFPVVDKRGVLLGVV
                     LLDDIRNIMFRQELYGRFKVTNLMISPPAVLKISNSMEEVMKIFDDTNAWNLPVKDDE
                     GLYVGFVSKSKIFNSYRRVLVHFSDE"
     gene            10503..11474
                     /gene="fmt"
                     /locus_tag="PLI37_03135"
     CDS             10503..11474
                     /gene="fmt"
                     /locus_tag="PLI37_03135"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOQ58043.1"
                     /translation="MRKQELKIVYMGTPEFAVAPLQALIDNHYNVAAVVTMPDKAMGR
                     GLKVQASPVKQLARSYSLPVLQPDNLKDPVFLEKLRSYDADLQIVVAFRMLPEAVWAM
                     PPMGTFNLHASLLPQYRGAAPIQWAVINGEKQTGLSTFLLDRQMDTGRILLQEKIAIG
                     PDETGGSVYSRLMELSGPMVLRTVDLLLSGQSRPVDQNTLIKEAKPLKTAPKLHKENT
                     RIDWSRPGAELYNFIRGLAPYPAAWTAWQKSGESIQTVKIHQAAFVAQEHHMECGKLL
                     SDQKTYIKTPVPDGFLHWEFFQPAGKRILSVAEFLRGRPGIEDFKLL"
     gene            11598..12191
                     /locus_tag="PLI37_03140"
     CDS             11598..12191
                     /locus_tag="PLI37_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ58044.1"
                     /translation="METHRQMTDEALVSRFANGDNQAFDILLNRHKNRVYTYILLIVR
                     NRDLAEDVFQETFMKAIVTIKQGRYVDSGKFYAWVTRIAHNLIIDIFRKERNENTISN
                     DEFEDVDLFNNPKFSDENVEDRLVKKQVMKDVSSLVRMLPDSQKEVVVLRYYKNLSFK
                     DIADQTGVSINTALGRMRYAVLNMRRMAEGKDLVLSF"
     gene            12525..13175
                     /gene="rpe"
                     /locus_tag="PLI37_03145"
     CDS             12525..13175
                     /gene="rpe"
                     /locus_tag="PLI37_03145"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOQ58045.1"
                     /translation="MRLVAPSLLSANFARLEADLTMINESQADWLHLDVMDGVFVPNI
                     SFGFPVLEAVARVCTKPLDVHLMIVEPQKFIPQVKALDAYLMNVHYEACTHLHRVVSE
                     IHQAGMKAGVTLNPHTPVSVLEDILPDLDLVLLMSVNPGFGGQVFIEQTYAKIVKLRQ
                     MIDDCKSPALIQIDGGVSLDNAGALYRAGADVLVSGSFIFKSPRPKEIIEQIKKAE"
     gene            13232..14857
                     /locus_tag="PLI37_03150"
     CDS             13232..14857
                     /locus_tag="PLI37_03150"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOQ58046.1"
                     /translation="MPQVFSYGMVSFVFQQAPFLRITISLISGMILAPFEFFPGLLFS
                     FILLLATVFLRLFTAKAGAAWRLRKLPGFLILLCCFCLGSALFRGRQQERACPKLENY
                     YLRAEFEILSNPQIKPNSLQCPVRIICSEPSCWKNKRCVLYLAKDSLALSLRAGNRIL
                     AQTTLRPYSRCGRPMEFNYERYLLNKGFCAGAFVPARAWMLQSRYSSPRAKSDAFRDR
                     LIGRFRQAGMEEEPLALVSALVLGCKDLMEAEQRQAFSSAGVAHILAVSGMHVSILFL
                     LLDKLLFFIKPWRAGLFFKQSLLIVLLWFYAFVTGMSASVLRAVLMCSVSALGLCLGR
                     RSNSWNTLAFSAFALLTVNPCYLTDVGFQLSFMAVASILLLMPGAEYYLGMITERSAS
                     VAKKSIKAKIVGIYRETLLLSLVAQLGTAPLSIYYFNQFPQYFLLGNVLLVPLATLLF
                     YLCFAFIVLSPLPWLSEVLVRPVNALASFFCRSSAYIENMRGSVVADLWPSKQDVLIC
                     YLLLALLFYLFKNKRYICALNKLISKLYKQISD"
     gene            14862..15908
                     /locus_tag="PLI37_03155"
     CDS             14862..15908
                     /locus_tag="PLI37_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOQ58047.1"
                     /translation="MISKIIDEDLIQKTNSLLQKCDNIVLITHEYPDGDGLGSALAMY
                     DYLSLEGKQVSVLLPNEIPSFLQWMPGADSVIVYEQDPETARSLLGQAEVIIFLDLNS
                     MERVGAMASFISQLTVHTVLIDHHNLREKPIAHVEIQLPQIASTCELVFRYICRVGAF
                     EKLSKDCAVCIYTGMMTDTGAFTYNSQSNEMYLIINQLIVKGIDKDEIYKKVYGNYTE
                     NRMRLMGYALYEKMEVLPVYQTAIITLSREELDRFGHQAGDTEGLVNQPLNISDVRFS
                     VFLREEEQNEIKISLRSEGDLSVSKIAADLFQGGGHFNASGATYRGSMQEALRIIKEA
                     LPNYLGTPEDEEDN"
     gene            15948..16517
                     /locus_tag="PLI37_03160"
     CDS             15948..16517
                     /locus_tag="PLI37_03160"
                     /inference="COORDINATES: protein motif:HMM:NF027435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4827 family protein"
                     /protein_id="HOQ58048.1"
                     /translation="MKYSILPCFLLAVALLSASCNKDVITYAEQLEAQQKSISKFIDN
                     NGFLVIDSIPSTVPWEDSEGRKMFYLSKSGLYLHVVDTGRQAGKTVNNTLVCVRYTEC
                     SMEGDTTYSNMDYATDPIEIYFNTVSTGTGSSTYYWGDCKAWHEPLNYVGDLGHVMII
                     APASVGMPYYSAGNRMLARYYDLRYTFWK"
     gene            16526..17908
                     /gene="glmM"
                     /locus_tag="PLI37_03165"
     CDS             16526..17908
                     /gene="glmM"
                     /locus_tag="PLI37_03165"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOQ58049.1"
                     /translation="MNQLIKSISGIRGVIGGTPENSLNPFNIVRFTAAYARWIRQNSD
                     RQSGKIVVGRDARISGEMLFQLVSGTLMSLGFDVLDIGLATTPTTELAVRMSGADGGI
                     ILTASHNPKQWNALKLLNQEGEFLDAAAGRFVCDQADSDRVEFAGVDQLGKIYREDFT
                     QKHIEAVLALPLVDVEAIYKAGFRVAIDCVNSVGGLVLPPLLRALGVQEIIELNCTPD
                     GHFPHNPEPLPQHLGEISSLLSRGGIDVGFVVDPDVDRLAIVCEDGSMFGEEYTLVAV
                     ADYILGRTPGNTVSNLSSTRALRDVTEKYGGKYFSAAVGELNVVAQMKAVNAVIGGEG
                     NGGVIYSESHYGRDALVGIALFLSHLAHKQQKVSELRAELPAYFMDKQRIDLSPGLSL
                     PLIFDTLKKRYAGEKITDIDGVKIDFSDRWVHVRGSNTEPILRIYAEAPSQEAANALA
                     TEVMELIHRL"
     gene            18001..19008
                     /locus_tag="PLI37_03170"
     CDS             18001..19008
                     /locus_tag="PLI37_03170"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HOQ58050.1"
                     /translation="MKKLALLVFLFLLLSCSGKKGNDSLQDDRQTSPAHSRKASQNDK
                     NRPAAQTEAPKTFIMPEIPPMLVESGSRTLYLLNHYWDNFDFSDTSYIGLKEITERAF
                     IHYAQMLSQRDVVPYDKAVEGMLTMLNKAQMDSAMYRYFTGLCKKYFYRGNSPMMNDE
                     YYIPVLEHMLQSPHLAPASKKAVELDLKRLKRNLPGQSAENFDYTLHSGKTQTLHQLK
                     AEYILLFFNNPDCPACKEVQEQISASPLLSKLIQKGSIKVLALYPDSDLASWDKHREQ
                     IPSAWINAYDKDQRIMLDELYDLKAIPMLYLLDGQKKVLIRDGKVKQVEQLLSAIIQQ
                     E"
     gene            19074..>19981
                     /locus_tag="PLI37_03175"
     CDS             19074..>19981
                     /locus_tag="PLI37_03175"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOQ58051.1"
                     /translation="MNNKLSILFGLGLMLLTSCSSGYTIKGKLAGNELDGQQVMIINL
                     ATDEAVDSGLVENNSFLIKGTVEEPAVCAALIGNKFSYFVLENARIALDMEKSFNASA
                     TPLGKSFEALYTAMNQLNESTNQKLGELAPEEQQAYYENEVIPASKVLAKEHLDKNLD
                     NTVGEFAYMLYSSFADPQELIDAYEAMGDNLKDNPQTLRIYQKSKSMLTTAEGMPFVD
                     FTVTNAQGTQSLSDYVGKGKYVLVDFWASWCGPCLQETPNIVELYEKYHKKGLDILGV
                     AVWDKPEDTQKKIEELGLPWPQIIDA"
BASE COUNT         4938 a         4616 c         4880 g         5547 t
ORIGIN      
        1 aggctggatt aacgcttcgg aatccctgtt tgaggcaaaa tctaaactca gcattgcccc
       61 caatgcccaa atacccgaag gagcggaatt gccgctgaac ttcaaggtgg tttcaaagca
      121 gagtcaggtt aaataaaatg gtcttccgac aggacaagac tcaggttgat ttaaacgata
      181 agttaagagg ttttattttc cgatgcaaaa gcgactaaag atttgtccta atacttcgtc
      241 gttgctgatc tgaccggtaa taagccccag gtggtacatg cattctctga tgtcctgagc
      301 gataaaatcg ccggacaagt tttgttgcaa accttcccga acgcgcctta tagcttccag
      361 gccggcggag agggcttcaa agtgccttat gttgctgact atggtatcgg ttttgtcaaa
      421 ttcgggcaga ttggttagtt ctatcaattt agatttgagt tcttcgatgt tttgtccgta
      481 tttggccgat atttgaatgc ggtcgccggg aatatgcgca aataagtttt cgacaatacc
      541 taactcttcg ccggcaatct tatcggtttt attgaagacg agcaagagct ttttcccctg
      601 actgcgagga ataatacgtt cggccatcca atcgatgtgc tcgctgagct gggtagaatc
      661 tatcagccag agaataatgg atgcctgttc tattttttga taggtgcgtt ctatgcccag
      721 gctttcgatg cggtctttgg ttttgcgtaa gcctgccgta tcgataaaac gaaaactaag
      781 gcctcttatg ctgaccaggt cttcgatgct gtcgcgggtg gtgccgtgta tgtcggaaac
      841 aatggctttg tcttcgttga gtaatttgtt gagcaaagtc gatttgccgg agttggtttc
      901 acccactatg gccaccggta tgccgttttt tatggcgttg cccaggctga aggattcgca
      961 aagggcagaa agtttggatt ccagttgatc ggccagcttt ttgagttcgt cccggctggc
     1021 gaaaatcagg tcttcttcgc tgaaatcaag ttccagttct atcaaggaaa caaaatgcag
     1081 caatttttcc ctgagatctg ccagatcctt ggagaaacct ccccgcatct gtttcagtgc
     1141 cagttgattt gccgaagaag aatctgctgc aatcaggtcg gctacggctt ctgcctgcga
     1201 caggtccatt ttcccgttca gaaaagcccg ttgggtaaat tctcccggcc cggcctgccg
     1261 gcaacctaat tgaattaaga gctgaatcag cctttgctga ataaagagag aaccgtggca
     1321 ataaaactcc accgtatctt caccggtaaa agaatttggg ccccggaata cgcttagcag
     1381 gacgtagtca agtactttat cggcctcttc catcagctgt ccgtattgac aactgtgact
     1441 cgcctgttcc ctaacagggc agccattttt accgggaacg aacaactgat cggcaattgc
     1501 aatggctttc ggtccggaga gcctgataac agcaattcct cccgaacccg gcggagtgga
     1561 gatggcacaa atagtatctt gtttcatagg tcttacaggc ggtcgagtac cagggttatc
     1621 agttcagaca tacggtcttt gtagttggtt ttggcatcac ttgcaaaacg atttactatg
     1681 accaggcaaa ccgtcatggc tttgtgtccc ataagagcag ccaggcccgc cacaccggca
     1741 ctttccattt cgtaattggt aatgcggtaa gagtcgtaac ggaagctgcg caggctctcg
     1801 ttgaatccgg gtagggctaa tttcaatcga agttgtcttt cctgggggcc gtaaaaaccg
     1861 ttggctgtta cggttacccc gcggagcata tctttttgcc ctatgcgggc aagcagttca
     1921 ggattggccg aaaccacata gggacgagcc cattgtttca ggtaagcagt atgattacaa
     1981 aaagcttctt caaatttcag gtcggaaacc ttgtcgcggc cggcataaaa attgagcagg
     2041 ccgtctaagc cgatggattt ttcggaaatc aggaagctgc ccaccggcaa gtcttcctgt
     2101 aggcctccgg tggtgcctat cctgactatg ctgaggcttt tcttttcggc tttttcggtt
     2161 cgcttttcaa aatcgatatt agccagcgca tcgagttcgt tcatcacgat atcgatgttg
     2221 tcggtgccga tgccggtgga aagtacggtc attcttttac cgtaataaga tccggtaaca
     2281 ctgtggaact cacgatttcg tacccggcat tccacaatat caaacttgga ggctaccagg
     2341 cttactcgat cgggatcacc taccagtacg acccgatcgg ccagttgttc cggcttaaga
     2401 cgaagatgaa aaatgcttcc gtcttcgttg ataatcagct ctgattcgag aaaatgcaaa
     2461 ctcatggcga tgaagtttat gccccgttta aagagcagtt aaactaagtg ataagggacg
     2521 ctatttcttg ggcgtcgggg gcttggcaat ggattccctc atgttggtat ccgactcgat
     2581 atttctcatc cggtagtaat ccattattcc caggttgccg ctgcggaaag cttccgctat
     2641 tgctttgggc acttcggctt cggcttcaat tactctggct ctggcttcct gagccttggc
     2701 tttcatttct tgttccagag caacagccat ggcacgtctt tcttcggcct tggcctgagc
     2761 gatgttctta tcggcttgag cctgatccat ttgcagttct gcgccgatgt ttttgcctac
     2821 atcaatgtcg gcgatatcga tcgagagtat ttcaaaagca gtaccggcat caaggccttt
     2881 gcgaagcacc actttggaga tagaatcggg gttttcgagc accattttgt ggttttccga
     2941 cgaaccgata gacgatacta taccttcacc aacacgcgcc agaacggtgt cttcaccggc
     3001 accacccacc aactgcttga tattggccct cacagtaacc ctggctttgg caatgagctg
     3061 gatgccgtct ttagcaacgg cagttaccgg aggagtgtcg ataactttgg gatttaccga
     3121 catttgtacg gcggtaaaaa cgtcccgccc ggccaggtcg atggctgtag ccatctgaaa
     3181 actaagatcg atattggctt tggatgccga taccagggcg tgaaccactt gttgcacgtg
     3241 acctccggcc aggtaatgtg cttccaaatc gtcgcgccgc agttctttga gtccggcttt
     3301 gtgtccctct atcagggcgt ttacaataat cgtgggagga actttacgta ttcgcatcaa
     3361 aaatagctga atcagagaaa tgtttactcc ggaaactttg gccgataacc agaggaaaaa
     3421 cggcacataa tagaagaaga taatcaaaag aatgattacc ggaatcaaaa taagcaaacc
     3481 aaatcctggt gacataatag tataatttaa aagtgaatac tcgttgtttt tatgttatga
     3541 cacattgtcg tcgtattttt tgacaataac gttcaaatcg gaaatcctta ttaccaacac
     3601 ctgagtgcct tcatccaata tttcgttttc gctgcgtccc tcgagagttt ttccgttgat
     3661 cagaatttta cccgtcggtg cgagccggga aatacaacgg cccacgtcac cgactttgac
     3721 ctctgcgcct tgatgggcag ctattttgga atcgatattg gtttccagcg acattttttt
     3781 gagcgttttg gaacgaataa accagacgaa agctatggcc atggccgcag cagaggaaat
     3841 cagagtaata tggccggccg taggtccgag gttggtgtag gccactatta tgcccccggc
     3901 aaaacaagcc aatccgccaa tgagggctac ggaaacaccc ggaaggaaaa aaatctccag
     3961 gatcataaaa aaaataccca atacgatcag gccaacgacc agagctatat tcatactgtt
     4021 ttaattgttt attggtgcat cgattttttc caactccaaa cgtcttattt catattcctt
     4081 gagtggaata tcccgttcca aaaagagcaa tacccactct gcatttaaaa ttctggaagc
     4141 caattcctgt tgttccggat tgtctttgcc cgtatacttg ggccggtcgg cttctaattg
     4201 ggtattcaag ctattgaaac gggcttgcaa cttcaaatat tggagatatg cctgacgggc
     4261 ttcctcgctc ctgaagtcgt tttcgttttc atacacgagg tcgtctctaa caaaaaagcg
     4321 aagttgttca ctaccggtat gttcgagctc atgcaatacg gtattgttgt cgtattgata
     4381 gcgttcctcc agcattgttt ggatagaatc ggtgtctatg ccgtcctgtg tcagagagat
     4441 agaggtaagg cgtgcatatt cgataatttc ctgttccgat ttaccgctga ggatttttcg
     4501 ggtgctttcg ggtatgaaag tgtaaattgt aacactgtct ttgccggcat agcggtctgt
     4561 aactaaaaaa cccctaccga catattcgtc aacgatatag aaatagtcgt tatagacaga
     4621 gttgaaaggc atgggtagag gctcgggagt aaaaaaggat tcggaggcaa ggtgataacg
     4681 actcacgtac aagtcgtagc cgcctatgga gttgtagccg tccgaagcaa aatagatggt
     4741 aatgccatcg ttgagcacat aagggaagcg ctcgttactt gcctgattta aactcttaac
     4801 caggtcgatt gtttcccaac tgttcattgt gcggttgagg ctgaaaatgt cccagccgtt
     4861 ttcggccgta gtgtcgtcgt aaaaaacaca gtctttacgc tctgtcaggt aggcaacact
     4921 cagggaatct cccttttcgg ggaacagctt tctgagagga agtacggtgc cgttagcgct
     4981 gtgtaatctg atgttttcga aaacagaatc catcggaacc accaggctgt caataaaaac
     5041 gacgtcctcg acggccttca gcatttgttt tgctttctcg gaagccttga gcatgcgcgc
     5101 cactttatct gaattggaac ttaagccgaa agcgatgtat ttatcaaggt gtccggcact
     5161 tttgtcgaac tggtattgcc tgtggtaata gtccgccaat aacaggctgg ccgttggaat
     5221 tttgtttttt tctgcgttta ccaggtatgg cagggccgct gttggattgc cggtatggta
     5281 gaggcaggct ccgtaccagt agtttaggtt gacgtctttg ggtttacgtt tgagttcctg
     5341 ttcgaaaacc ggtagtgctt tttcatattc accgttgata tacatagccc tggcttctgt
     5401 cagggtttga gccgacaggg gaacggtaaa aaggaggaat actataagca ggtttctcat
     5461 gtccggactg aatcagttac aaaaatacaa aattaaaaac ggattatccg gcggcagact
     5521 gtatttttgc ggaaataatg tctatgactc ttttggagcc tgctcggaaa ttaagtaagg
     5581 gactcgattt tcgcgtaaag caagccatcg acaagtatac tcttattcag gacggcgatt
     5641 tgtccgcttt atctgccttg tgccgggatc tgagtgtaaa attccgcttc aacaatactc
     5701 agccgggcct gatcgccgtc gcctaggctt tcgatgcgaa cgttgcgggt aggtgaagcg
     5761 ggatttaggc tcagattgac gtagcccagg gtcttttctg tttgtccttc ccagagtact
     5821 tcgttttgct cggtaagaat tctcaggtgg tagctggtat tcctaaagtt accaagttta
     5881 aggcaggctt cggttagtac cgcactgcgc gaaagagtgt aatcgatcca gacttctccg
     5941 gccgggccgc gggcatccca ccggctcaat tcattgtcgt caaagctcaa ggctgcttcc
     6001 tcgggattac tgccggcgac agccgattgt atgctgagcg gaagtctcga catcacaaag
     6061 gatgcatagc ccggcgtagc tccccgccac aggtaagaaa tcaattcgtc cttgggaaag
     6121 aaagtactca gaccgtcttt taccggaact tccaggctgt taagcacgag agaatcaacg
     6181 ctcagaccct ctgcgtttgc ccggactaca atttttccgg cttcggtggt cgacctaagc
     6241 aaaattctgt ttactccgca ttctaccggt aaatcgcgcg aaagaatgta gttgttttct
     6301 ccctgggcga tgcctcccct gtattcggcc ggaccttcaa cgtcgaagtg aatcaggtga
     6361 ttggccaggg ggcagcgcag gccgtttttg tcaacaactt cgacttggat caaaaccatg
     6421 tccgatccgt ctgcacgaaa acctttagga tgttgccagt attggaggcg cagggcggcg
     6481 gcttcgtctg cggttttaat ttcggcaggt tctcctgcca attgtccccg gctgtcgtaa
     6541 ctgagggctt ttaggctgcc ggcttcgaag ggaacatctt tgaatttgaa caggaaatga
     6601 tgctgacgct ccccaaagcc caggttcctg ccgttcagga acaattcaac ggcagaggca
     6661 ttggaaacca ccagcacatc tttcacggtg cccggctcat agttccagtg gcctattata
     6721 tggttgctgt atttattgcc ttctacctca acccagccgt cccacattat tttgtgagcg
     6781 taataagcct ctttttctat gcgcatggca tctacctcgc cactgcggcg atagttttcg
     6841 gctccgcgga aatgagtgtt ggtgtcggaa aatataatat tgacacctcc ggcacttact
     6901 cgccttcctg tccccgggcg gtgtacccag aactcgtgcc agcgccgcac ggcctcttcg
     6961 gcaaaggagt cctggttgcg attgtaggcc gaggcgtctt cgtttctgta cagggggcct
     7021 tctccgtcgc ggtggtaggg ataggaataa ttgtcccagt acttgcgcag gccttcgtcg
     7081 cggcaatatt catgggcaaa catcggtatt ccggcgcttt tgttgatgta aagcatttcg
     7141 ccgccgtatt cggcttcttt actgtcgagc atttcgcgcg atcctatggc tctgccgccg
     7201 ttgggatcgt atatgtcgcg gatgaccttc atttcggcca tgtgttcctc gctgatggtc
     7261 tcattgccgc attcgtaaaa gataatactg ggatggttgc ggttgtagat gatggcatct
     7321 cgcataagct ctttgcgtaa ttcccaggaa cgtccctcta cgtcactttc ggcatcgccg
     7381 gcgggcatcg cctgcaacag gcctacacgg tcgcaggatt ctatgtcttg tttccagggg
     7441 gtaacgtgca tccagcgaac cagattggca ttgcctcttt ccataagtcc gttgctgaaa
     7501 tcgctcaacc agggaggtac cgacatgccc acagccggcc attcgttgct gctgcgttgt
     7561 gcatagcctt tcaggtgcaa caccctttcg ttcaggtaaa acatgccgtt gcggaaagcg
     7621 gttttgcgta ttcctgtttg ggttctgacc aggtccaggg tttttttacc gcttatcaac
     7681 gaagaagtta cattataaag gtagccgtaa ccccaactcc agaatcttaa ggccttaaga
     7741 ttgcctgaag ctttgacgat gcggctttct cccggttgaa gagtgatttc gcctgagttg
     7801 aattcgcgga tcatgttgtg gtcgttgtct cttactatga ccttcatata gaaagtttgc
     7861 gctttttcct gctcattctt cacttccgat tctacgttga gcacggctgt tttgtctctt
     7921 acattgatgt ctgtggcgta tacgtaggtg cccacagtac ccagatgact gtacaagggg
     7981 agcgtctggt agagtttgcc gcaaatatgc aaaacaacgt ttttggtcaa gcctccgtaa
     8041 ttggcgttga aattgttgtt gttccactga aagccggtac ctgtggcctt ttctttgtat
     8101 ttccaatcgt tgtctatcct gagggcgatt acgttttctt tgtttccgaa aaccagatga
     8161 tccgtcaggt ccagaccgaa agccatcact ccgttttcgt gcaggccaat ctcccggccg
     8221 ttgatccata tttcggcagc ctgacgcagg ccttcaaatt caatgaatac tttttgattt
     8281 ttgtaagtgg agggtatttt aaaatgtttg cgataccata cgacggtatc ggtcaaatcg
     8341 tggatggatt tggcaaaagc ttcatcctcg ttgaaggcag cgggcaggtg aacggttttc
     8401 cattcagtgt cgtcaaattc cggattatga gcgccgggtt ggtcgccgat gtggagcagc
     8461 caggagggat tgaagttaaa cttaatgcgg gcgggatttg ccgccaaagt tgtgccggca
     8521 attaaaactg cggcgaaaaa aagagcggct gtttttctgc gtgtgttcat gaacttaact
     8581 attggaatat acctcgaaac agacgggtga tagcaggttt ttttaacgaa gggcaaactt
     8641 acataaaaag cggttaaaac tgaaatcatc ttgcagattt gaattaaaat gatgtccttt
     8701 gccggcaacg aaatttctcg aatgagcgaa aaagattggt atatcaagat gattctttgg
     8761 agggagaagc acatcaaaga atccaacttc attctgatac tgagctttgt ggtaggcttg
     8821 ctcacatcgt tggcagccct gttgctgaaa acccttatcc atcagataca tacctgggtt
     8881 tcttcgggat cggccattga ccacgctaac tattattact tactgtatcc ggctctgggt
     8941 atttttttga cagctctgtt tgtccgctac gtggtaaggg acgacatcgg acacggagta
     9001 accaagattc tgtacgccat ttcacgtcgc cagggcagga tcaaacgaca taatacctgg
     9061 acttcgatgg tttccagctc cattaccatc ggtttcggtg gttcggtagg agcagaagca
     9121 cctatagtgc taaccggcgc tgccattggt tccaacttag gaagtttctt caaaatggac
     9181 cacaagaccc ttatgatttt gatcggttgc ggagccgccg gaaccattgc cggtattttc
     9241 aaggcgccca ttgccggact cctgtttgcc atcgaggtgt tgatgatcga tttcacggta
     9301 gcctctgtta tgccgatgct ggtatcttca gtgaccgcgg ctaccctggc ctactttatg
     9361 tcgggtaccg gtgtggccat ttcttttgac ggtgccgatg tttttcaact ggtcaatgta
     9421 cactgggtta ttgtcctggg cattttttgc ggcctggtct ccttgtacgt gatccggggt
     9481 atgaatacca tcgaaggctg gtataaaaaa atcaaaaatc cctggttaaa gatcatatta
     9541 ggagctttgg ctctgggctt acttatcttt ttgttccctc ctttgtacgg agaaggttac
     9601 gagagtatag aggcgcttat caacggcaat ctgaccgagc taactcacaa cagcctcttt
     9661 tatggtgtcc agcaacattt cggctggcta gctctttatt tgctgctcat catcgtattt
     9721 aaggtttttg cctcggcttc gaccaatggc gccggcgggg taggcggagt ttttgctccc
     9781 agcctgtttg tgggaagttt caccggcttt ctggtggcta cagcaattaa ttatttcggc
     9841 ctgggcaccg tgtctccggg taattttgcc ctgatgggca tggccggact gatgtcgggc
     9901 gttatgcatg ctccgcttac ggggatgttc ctgattgccg aacttaccgg aggttatgat
     9961 ctgttcctgc ctttgatgat agtggcttcg gtttcgttta tgaccatctt actgtttgag
    10021 aaacacagta tttactccat gcggctggcc caaaaaggcc aactgctgac ccatcacaaa
    10081 gataaggccg tgcttacgtt gatgagaatc gaaaacctga tcgaaaaaga ctttgatgtg
    10141 ctcagtcccg atatgaccct gggagatttg gtaaaggtga tatccaaagc caaacgcaat
    10201 atgtttcccg ttgtcgacaa acggggggta ttgttgggtg ttgttttgct ggacgatatc
    10261 cggaacatta tgtttcgtca ggaattgtac gggcgtttca aagtgaccaa tctgatgatt
    10321 tcgccgccgg ctgtactaaa aatcagcaac tctatggaag aggtaatgaa gatatttgac
    10381 gataccaatg cctggaactt gccggtaaaa gacgatgagg gcctctatgt gggttttgtg
    10441 tccaaatcca agattttcaa ctcctatcgc agggtgttgg tccatttttc cgacgagtaa
    10501 ttatgcgaaa acaagagttg aagatagtgt atatgggtac gccggagttt gcggtagccc
    10561 ccctgcaagc tttgattgac aaccattata atgtagcagc cgtagtcact atgcccgata
    10621 aagccatggg gcggggcctt aaagtgcagg cttcgccggt aaaacaactg gcccggtcat
    10681 actccttgcc cgtgctgcaa cccgataacc tgaaagaccc cgtttttttg gaaaaactgc
    10741 gctcttacga tgcggatttg cagatagttg tggcttttcg gatgttgccc gaagccgtct
    10801 gggccatgcc tcctatggga acttttaacc ttcatgcttc cctcttgccc cagtacaggg
    10861 gagctgctcc catacaatgg gctgttatca acggcgaaaa gcaaaccggt ctaagcactt
    10921 ttttgctcga ccgccaaatg gacaccggcc gcattctttt acaggaaaaa attgccattg
    10981 gtcccgacga aaccggcgga tccgtttatt cccggctgat ggaactttcc ggccccatgg
    11041 tgctccgtac cgtagatttg ctcttgagcg gccaaagccg gcctgtggac cagaatacat
    11101 tgatcaaaga agcaaaaccc ttaaagacgg ctcccaagtt acacaaagaa aatacacgta
    11161 tcgactggtc gcggcccgga gccgagcttt ataattttat tcgcggcctg gctccctatc
    11221 cagccgcctg gacagcctgg caaaaatcag gcgagagcat tcagacggta aaaatccatc
    11281 aggctgcttt tgtggctcag gaacaccata tggaatgcgg caaactgctg agcgatcaaa
    11341 aaacctatat aaagaccccg gtacccgatg gttttttgca ctgggaattt tttcagcctg
    11401 caggaaaaag aatcctttcg gtagccgaat ttctgagagg caggcccggt atcgaggact
    11461 ttaaactgct ctgacgacaa aaaaatgctt aaatacttgc agatttaaaa aaatcctctt
    11521 tcatttgcag cactaaccat taaaaccatt tgcctattga tccaatacta ctctttttaa
    11581 cgttttaaaa aagagagatg gaaacacacc gacagatgac cgatgaagca ttggtcagca
    11641 ggtttgcgaa tggagacaac caagcttttg acattttatt gaatcgccac aaaaacaggg
    11701 tgtatactta tatactgttg attgtccgta atagggattt ggcagaagat gtatttcaag
    11761 aaacttttat gaaggccatt gtaaccatta aacaaggccg ttatgtggac agcggcaagt
    11821 tttatgcctg ggttacgcgt atagcacaca atctgatcat cgatattttt cgcaaagaac
    11881 gcaacgagaa tactatttcg aacgacgagt ttgaagatgt ggacttattc aacaatccta
    11941 agttctcgga cgagaacgtc gaagacaggc tggtaaaaaa gcaggtaatg aaagacgtca
    12001 gcagtctggt tcgcatgttg ccggacagcc aaaaagaggt ggtggtattg cgctattaca
    12061 aaaacttaag ctttaaggat attgccgatc agaccggtgt cagtatcaac acagccttgg
    12121 gccgcatgcg ctatgcagtg ttgaatatgc gccggatggc cgaaggaaaa gacttggtat
    12181 tgtcttttta aaccacagat aaacaagcag ttatatcaaa agttgttttt tggtgtaaaa
    12241 aaaagcgttt gttttatacg atggaacaaa aatcctcgtt atagaataaa cgctttttta
    12301 attatacgcc tatggaaaaa atttctacac cgcgaactca atcatcagtg aaaaataaat
    12361 cagtctttct gatagaaccc aaagcatcca ccctggattt tttacgtata tttgccagag
    12421 tttaccccaa caacgaatta gcagtaccgg gtaaatctgt gttaagttta aattaaacca
    12481 agtcctttta atcagggctt gtatttcgta actaattttc caccatgcgt ttggttgctc
    12541 cttctttgct gtctgccaat tttgcccgtc tggaggcaga tcttacgatg attaacgaaa
    12601 gtcaggccga ctggctgcat ctcgatgtaa tggacggcgt gtttgtgccc aacatttcct
    12661 tcggcttccc tgtgctggaa gctgttgccc gcgtttgtac caagcctttg gacgtgcacc
    12721 tgatgatagt ggaacctcaa aagttcattc ctcaggttaa ggccctggat gcgtatttaa
    12781 tgaatgtaca ctacgaagcc tgtacgcatt tgcaccgtgt cgtcagcgaa attcatcagg
    12841 caggcatgaa agccggtgtt actctcaatc cccatacgcc ggtttcggta ttggaggata
    12901 ttttgcccga tctggacctg gtactgctga tgtcggtcaa tcccggattt ggaggccaag
    12961 tctttataga gcagacttat gcaaaaatcg ttaagctcag gcaaatgatc gatgactgca
    13021 agagtccggc tctcattcag atcgacggag gtgtcagcct agataacgcc ggggctttat
    13081 atcgtgcagg tgccgatgtg ttggtttcgg gcagttttat tttcaagtcg ccccggccca
    13141 aggagatcat cgagcaaata aaaaaagctg agtaagagag tttgaaataa aacctcttaa
    13201 aggtttccga aacgttaata aataaatatc tttgccgcaa gtattctctt acggcatggt
    13261 atcttttgtt tttcaacaag caccctttct gcgcattacg atttccctga tatcgggaat
    13321 gatccttgcg ccttttgagt tttttccggg cttgttgttt tcctttatct tgctgctggc
    13381 gactgtgttt ctccggctgt ttaccgctaa agccggagcg gcttggcgtt tgcgcaaact
    13441 gcccggcttc ttgattttgt tgtgctgctt ttgtttaggt tctgctcttt ttcggggcag
    13501 acaacaggaa agggcctgtc cgaagctcga aaattattac ctgagggctg aattcgaaat
    13561 tttgtccaat cctcaaatta aacccaacag tctgcaatgc cctgtcagga taatttgctc
    13621 cgagccttct tgctggaaaa ataaacgttg tgtgctttac ctggccaaag acagcttggc
    13681 tttgtcgttg agagccggca accgtatttt ggctcagact accttgaggc cctacagtcg
    13741 gtgcggcagg cctatggaat ttaattatga gcgatatttg ttgaataaag gcttttgtgc
    13801 gggagctttt gtgcctgccc gggcctggat gttgcagagt agatactcta gcccgcgggc
    13861 aaaatcggat gctttccgcg atcgcctcat cggccgtttt cgacaggcag gaatggaaga
    13921 agagcccctg gctttggttt cggcactggt gttgggttgc aaagacctga tggaggcaga
    13981 gcaaaggcag gctttttctt cggcaggcgt agcccatatt ctggccgtaa gcggaatgca
    14041 tgtgtctatt ttgtttctgc tgctcgacaa gttgcttttt tttattaagc cgtggcgtgc
    14101 cggcctcttt ttcaaacaaa gcctgctcat tgttctgctt tggttttatg cctttgttac
    14161 gggcatgtct gcgtcggtac tcagggcagt gctgatgtgt agtgtttctg ctttggggct
    14221 ttgtttggga cgacgttcaa acagctggaa tacgctggct ttctcggctt ttgcactctt
    14281 gacagtcaat ccctgctatt taacggacgt cggttttcag ctgagtttta tggctgttgc
    14341 ttccattctg ttattaatgc cgggtgccga gtattatttg ggcatgatta cggagcggtc
    14401 tgcctcggtt gccaaaaaaa gcataaaggc caaaatcgtt gggatttatc gcgaaaccct
    14461 gctgctgtct ttggtcgccc agcttggaac cgctccgctt tctatttatt attttaacca
    14521 atttcctcag tattttttgt tgggcaatgt tttgttggtt cccctggcta ccctcctgtt
    14581 ttatctctgt ttcgctttca ttgttttaag tcctttacct tggttgagcg aggttctggt
    14641 ccggcctgta aatgcacttg caagcttttt ttgccgcagc agcgcttata tagaaaatat
    14701 gcgtggatca gtggttgccg atctttggcc ttcgaaacag gatgtgctga tttgttatct
    14761 gcttttggcc ctgctttttt atctgttcaa aaataagcgg tatatttgcg ccctcaacaa
    14821 gcttatatct aagttgtata agcaaatttc cgattaaaac tatgatatcg aagattattg
    14881 acgaggacct gattcaaaag acaaatagct tattgcaaaa atgcgacaat atagtgctga
    14941 ttacgcacga ataccctgat ggcgacggcc tgggttcagc tttggctatg tacgattatt
    15001 taagtttgga gggaaaacaa gtcagtgtgc tgctgcccaa cgaaattccc tcttttttgc
    15061 aatggatgcc cggtgccgat tccgtaatag tgtatgaaca ggatcccgaa actgcgcgca
    15121 gtctgctcgg acaagcagaa gtgataatat tcctggatct caacagcatg gaaagagttg
    15181 gagccatggc ctcctttatt agtcaactga cggtacacac ggtgttgatc gatcaccaca
    15241 acctccgcga aaagccaatt gcgcatgtgg aaatacagtt gccccaaata gcttcaacct
    15301 gtgagttggt gtttcgctac atctgcaggg tgggggcttt cgaaaaactg agcaaagatt
    15361 gcgcagtctg tatttataca ggaatgatga cagatacggg agcttttacc tacaattctc
    15421 aatccaatga gatgtatctg attataaatc aattaattgt aaaaggtatc gacaaggacg
    15481 aaatttataa aaaagtctac ggcaattaca ccgaaaatcg catgcgcctg atgggctacg
    15541 cgctctacga aaaaatggaa gtcctgcctg tatatcaaac agccattata acgctgagcc
    15601 gcgaagagtt ggatcgtttt ggtcatcagg ccggcgatac cgaaggtctt gtaaatcagc
    15661 cgcttaatat aagcgatgtg cgcttttcgg tgtttttacg cgaagaagaa cagaacgaaa
    15721 tcaaaatttc cttgcgttcc gagggcgatc tgtcagtctc aaaaatagct gcggatctct
    15781 ttcagggggg cggtcatttc aatgcttccg gcgccactta ccggggcagt atgcaggaag
    15841 ccctgcgaat aatcaaagaa gctttgccca actacctggg aactccggaa gatgaggaag
    15901 ataattaaaa aaaacctacc tttgcgctct tattcaacgg atttagtatg aaatactcca
    15961 ttttaccctg ttttttgctg gctgttgccc tattgtcggc ctcctgcaac aaggacgtca
    16021 ttacctatgc tgaacaattg gaagcccaac agaagagcat aagcaaattt atagacaaca
    16081 atggattttt agtgatcgac tctattccct ccactgtgcc ctgggaagac agcgaaggtc
    16141 gtaaaatgtt ttatctgagc aaaagcggac tctatctcca cgtggtggat accggcaggc
    16201 aggcaggtaa aacagtcaat aatacgttgg tttgcgtacg ttatacagaa tgttccatgg
    16261 agggagacac tacctactcc aacatggact acgccaccga tcccatcgaa atttatttca
    16321 acaccgtatc caccggtacc ggttccagta cctactactg gggcgattgc aaagcctggc
    16381 acgaacctct gaactatgtc ggcgacctgg ggcatgtaat gataatagcg ccggccagtg
    16441 taggtatgcc ttattattcg gccggcaata gaatgttggc gaggtattac gatctaagat
    16501 atactttttg gaagtaaata attttatgaa tcaattaatt aaatctattt cgggtatcag
    16561 gggtgtgata ggcggaacgc cggagaatag cctgaatcct tttaatatcg ttcggtttac
    16621 ggctgcttat gcgcgctgga ttcgtcagaa ttccgacaga caatccggaa aaatagttgt
    16681 gggacgcgat gcccgcatct cgggcgaaat gctttttcaa ctggtttcgg gtactttgat
    16741 gagtctgggt ttcgatgtgc tcgatatcgg cctggctacc acgcctacca ccgagctggc
    16801 ggtacggatg tcgggagccg acggaggtat tattctcaca gccagtcaca acccaaaaca
    16861 gtggaatgcc ctgaaactgc ttaatcagga gggtgaattt ctcgacgcgg ctgccggtcg
    16921 ttttgtttgt gaccaggccg acagcgaccg ggtggaattt gccggagtag accaattggg
    16981 caaaatttat cgcgaagact ttactcaaaa acatatcgaa gccgtgctgg ccttgccttt
    17041 agtggatgtt gaagctattt ataaagccgg gttccgtgtt gccatcgatt gcgtcaattc
    17101 ggtgggcggc ttggtgttgc ctcctttgct cagggcccta ggcgtacagg aaatcattga
    17161 actgaattgt actcccgacg ggcatttccc gcataatccc gaacccttgc cccaacatct
    17221 gggcgagatt tcctccctgc tcagtcgggg cggtatcgat gtgggcttcg tggtcgatcc
    17281 agacgtagat cgcctggcca ttgtttgcga agacggttcc atgttcggcg aagaatacac
    17341 tctggttgcc gtagctgatt acattctggg ccgtacgccg ggcaataccg tttccaatct
    17401 gagttcgacc cgcgccttac gcgatgtgac cgaaaagtac ggcggcaagt atttttccgc
    17461 tgccgtggga gaattgaacg tggtcgccca aatgaaagcc gttaatgccg ttataggggg
    17521 cgaaggcaat gggggagtga tttattccga atcgcattac ggtagagatg ccctggtggg
    17581 cattgccttg ttcctttcgc atttagctca caaacagcaa aaagtatccg aactgcgggc
    17641 ggaattgccg gcttatttca tggacaaaca acgaatagat ttgagtcccg gcctaagttt
    17701 gcctctgatt tttgatacac ttaaaaaacg ctatgccggc gagaagatta ccgacatcga
    17761 cggggtcaaa atagatttca gcgaccgctg ggtgcatgtg agaggttcca acaccgaacc
    17821 gattctgcgc atttacgccg aagctccctc tcaggaagca gccaatgctc tggcgacaga
    17881 agtcatggaa ctgatacacc gcctctaaag tggagcgttt tgtgcccatt tcgatggcgg
    17941 gggccggatg aaaagccggt ggaggtacta taatgcccgt aacaattaat gtctggaaca
    18001 atgaaaaagc ttgcattgct cgttttcctc tttttgttgc tgtcgtgcag cggcaaaaaa
    18061 ggcaatgatt cattgcaaga cgatcggcaa acttcgccgg cgcattcccg caaggcaagc
    18121 caaaacgata aaaaccggcc tgctgcacag acagaagccc caaaaacttt tataatgccc
    18181 gaaataccgc ccatgctggt ggaatccggc agccggacct tgtatttgct gaatcactat
    18241 tgggacaatt ttgattttag cgacacttct tacatcggtt taaaagagat caccgaacgg
    18301 gcttttatcc attatgccca aatgctcagt cagcgcgatg ttgttcctta cgacaaggca
    18361 gtcgaaggca tgctgaccat gttgaacaag gctcagatgg acagcgccat gtaccgttat
    18421 tttacggggc tctgcaaaaa atatttttac cggggcaatt cgcccatgat gaacgatgaa
    18481 tactacattc ccgtattgga gcatatgttg caaagtcctc atttggctcc cgcttcaaaa
    18541 aaggctgttg agctcgattt aaaacgcctc aaacgcaatt tgccgggaca atcggccgaa
    18601 aatttcgact acactttgca ttcgggtaaa actcaaactt tacaccaact aaaggctgag
    18661 tatatcctgc tgtttttcaa taaccccgat tgcccggcct gcaaagaggt gcaggaacaa
    18721 atttcggctt cgccgctctt gtcaaagctt atccaaaagg gcagcatcaa agtattggct
    18781 ctataccccg acagcgatct ggcctcctgg gataaacacc gggagcagat tccctctgcc
    18841 tggataaacg cttacgacaa agatcaacgg atcatgctcg acgaattata cgatctcaag
    18901 gccattccca tgctctactt actggacgga caaaaaaagg tcctgatcag agacggcaaa
    18961 gtgaaacagg tggaacagct tttatccgct atcattcaac aagagtagtg attattcagg
    19021 aatagcaatt cattactaat tattttaaaa caaccaatta aaaacaatca agcatgaaca
    19081 acaaactgag tattttattt ggcctgggcc tgatgctgct cacatcctgt agctccggct
    19141 ataccatcaa gggcaaactg gccggcaacg agctggacgg acagcaagtg atgattatca
    19201 atctcgccac cgacgaagcg gtggacagcg ggttggttga aaacaacagc ttcctgatca
    19261 aaggaactgt agaagaaccg gcggtatgtg ccgctcttat cggcaataaa ttttcttatt
    19321 tcgtgctcga aaatgctcgt attgctctgg atatggagaa gtctttcaat gcttcggcca
    19381 ctcccttggg caaatctttt gaggcgcttt atacggccat gaatcagctc aacgagtcta
    19441 ccaaccaaaa gttaggagaa cttgctcccg aagagcaaca ggcttattac gaaaacgagg
    19501 tcattccggc ttctaaggtc ttggccaaag aacacttaga taagaactta gacaatactg
    19561 tgggtgaatt tgcctatatg ttgtattctt ccttcgccga tcctcaggaa ttgatcgatg
    19621 cctacgaggc tatgggcgac aatctaaaag ataatcctca gactctgcgc atttaccaaa
    19681 agagtaaatc gatgttgacc acggccgaag gcatgccctt cgtggatttc accgttacca
    19741 atgcccaggg cacgcagtcg ctttcggatt acgtgggcaa aggtaagtat gtgttggtcg
    19801 atttctgggc cagttggtgc gggccttgtt tgcaggaaac gcctaatatc gtggagctgt
    19861 acgaaaagta ccacaaaaaa ggcctcgata ttttgggtgt tgctgtttgg gataaacccg
    19921 aagatactca aaagaagata gaagaattgg gtcttccctg gccccagatt attgatgcac
    19981 a
//
LOCUS       DAOSTD010000028        19707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_2926_length_19707_cov_17.200743, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000028 DAOSTD010000000
VERSION     DAOSTD010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19707
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2926_length_19707_cov_17.200743"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="PLI37_03180"
     CDS             complement(<1..1388)
                     /locus_tag="PLI37_03180"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOQ58052.1"
                     /translation="MKDSGISMNPNELVQFLNKPSQDFTRKDIMRFCEERQIKMLNFR
                     YVAEDGRLKTLNFVINSRDYLETILTRGERVDGSSLFSFIESGSSDLYVVPRYRTAFV
                     NPFAEVPTLDIFCSFYNNHGRPLESDPYTILHRADQLFTKETGSRFMALGELEYYVIA
                     PAGGLYSGVDQKGYHASEPFAKYEFIRNEALQMIAMAGGNIKYGHSEVGCFTQGESYY
                     EQHEIEFLPCTVEKAAEQLVLAKWILRMLGNKYGVGISYAPKITEGKAGSGMHFHMML
                     EKDGKNLVAGKGGLSDVARKMIAGILDLSDAITAFGNTIPTSYFRLVPNQEAPTRICW
                     GDRNRSVVVRVPLGWTGAEKMIFDANPLESEPLPASESRQTFEYRVSDGSADIFLTLA
                     ALIVASLHGIRRPDALELAEQLYVDVNIFKPEHREKMQSLEQLPLCCADSAEALKRKR
                     AVFEENNIFPAGL"
     gene            complement(1467..>2120)
                     /locus_tag="PLI37_03185"
     CDS             complement(1467..>2120)
                     /locus_tag="PLI37_03185"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOQ58053.1"
                     /translation="IGTNGEMALVKGEEIFTCATAAGPAFEGAGLSSGMPALKGAIAA
                     FESPSNFKVIGDIEPVGVCGSGAIDVIAWLRRNHLMDESGLIQQDFEIYPGIQITQAD
                     IREIQLAKSAIYSGIKILLKRAGLNFQELEALFLAGGLGNYIHIPSAVEIGLLPAELK
                     DKTFAVGNSAGIGALQFLRSEVFANKVHRVVQQSQYIELSDDEDFALEFAMNMDFLK"
     assembly_gap    2121..2220
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2221..2672)
                     /locus_tag="PLI37_03190"
     CDS             complement(<2221..2672)
                     /locus_tag="PLI37_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOQ58054.1"
                     /translation="MYKILLRENSQTRVIQTQGQQSLLEILRNEDYSIYAPCGGKGRC
                     GKCLVDIKGEGKVLACNYYPAGDIEVILPAKEEAQILATQTAYLEDFPPAHSRQLLHP
                     DAYGAAIDLGTTTIVCYFVHLASGRIVKISSWMNPQTAYGADVISRIS"
     gene            complement(2665..3582)
                     /locus_tag="PLI37_03195"
     CDS             complement(2665..3582)
                     /locus_tag="PLI37_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOQ58055.1"
                     /translation="MTKIDRSKMGLRLIVLIATVIIALFLSSLLLNLFLLLSRGLSFG
                     RLGSELGAQMSNPSFLLLMQGLQTLLVFILPPFLLSYWYGEKNSRFLKLVKAKPVDVL
                     LAMFSILAAVPLVNFLVSWNQDIQFPSFLEGVEKWMRVAEDNAQRVTELMLQGESWSD
                     LLWQLLVVAVMAGFGEELLFRGTLQSIISSKGRIQLAIWITAFVFSLIHMQFYGFIPR
                     LLLGAWFGYLLLWSGSIWVPVAAHFANNAISVVFHYAEKNNYLASDPELLGVGENTWQ
                     LIPSILLLTACGLFFYLRYRKSTLKKQHV"
     gene            3747..5105
                     /gene="tig"
                     /locus_tag="PLI37_03200"
     CDS             3747..5105
                     /gene="tig"
                     /locus_tag="PLI37_03200"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOQ58056.1"
                     /translation="MNVEKQLIDPVNASIKINLVKADYQEKVDKTLKTYRQKANVPGF
                     RPGMVPLGLIQKMYGKAVLAEEINKIVLEALYNYIKENNLNILGEPLPGKQQTPPDFN
                     AGEDFEFIFDIALAPELDFTISKKDSIPYYKIEVSDDMLEKQIKSLASRGGRYEKVEE
                     AQEGDTIKGTLTEIPEEGQKDSEDLVKVEEAIILPAYFKNEEEKAKMKGVRPGDKIRF
                     NPYKAAEGHETELASMLHLPKEKAVEIKSDFEFEVKEISRFKEAELNRELFDQIYGKD
                     VITSEEAFRTKVREEIEARFAPESDYRFMVDAEKQLVKKLKDVEFPVEFLKRWLLAAD
                     EKRNPATIDQDMPKLVEELKWHLMKEEIIKRNNIQVSEADVLETAKKATRAQFAQYGM
                     MSVPDDLLNNYAAEMLKKPETARNLSDQTNSAKVADFLKSAVKLNEKTVSAEEFNKLY
                     EN"
     gene            5111..5776
                     /gene="clpP"
                     /locus_tag="PLI37_03205"
     CDS             5111..5776
                     /gene="clpP"
                     /locus_tag="PLI37_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOQ58057.1"
                     /translation="MNDFRKYATRHLRMDGMALDQYSKITSSYISPTIIEERQLNVAQ
                     MDVFSRLMMDRIIFLGTQVDDYTANVVQAQLLYLDSAEPGKDISLYLNSPGGTVYAGL
                     GIYDTMQFISSDVSTICTGVAASMAAVLLVAGTKGKRFALKHSRVMIHQPMGGVQGQA
                     SDMEITVREIQKIKKELYSIISSHSGQTIEQIEKDSDRDYWMTAAEAKDYGMIDEVML
                     KSK"
     gene            5779..7014
                     /gene="clpX"
                     /locus_tag="PLI37_03210"
     CDS             5779..7014
                     /gene="clpX"
                     /locus_tag="PLI37_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOQ58058.1"
                     /translation="MAKTSTDKCSFCGRSRSEVELLISGISAHICEECAERAHSISKE
                     IIGSSKNKQFELKAEDLPKPKEIKAFLDLYVIGQEEAKKFLSVSVYNHYKRLMQTKKN
                     DEVEIEKSNIIMVGATGTGKTLLAKTIAKKLKVPFTIVDATVLTEAGYVGEDIESLLT
                     RLLQVADYDVAAAEKGIIFIDEIDKIARKGDNPSITRDVSGEGVQQGLLKLLEGSVVN
                     VPPQGGRKHPEQKMIPVNTQHILFICGGAFDGIEKKIAARLNTQVVGFGASKQVARID
                     RENLLQYIAPQDLKAFGMIPEIIGRLPVLTYLEALDRKALRAILTEPKNSIVKQYIKL
                     FEMDGVTLSFDDDVLEYIVDKAIEFKLGARGLRSIAETIMMDMMYETPSGRRKNIRID
                     LKYAQSKIEAANQSRLREQ"
     gene            7110..9290
                     /gene="recQ"
                     /locus_tag="PLI37_03215"
     CDS             7110..9290
                     /gene="recQ"
                     /locus_tag="PLI37_03215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOQ58059.1"
                     /translation="MAKSNPLAESLKLHFGFDTFKGNQEAIIRNIMEGKDTFVLMPTG
                     GGKSLCYQLPSLLLNGTAIVISPLIALMKNQVDAMRNFSDEDGVAHFINSSLNKAAID
                     QVRNDITSGKTKLLYVAPESLTKEENIDFLKQIPISLFAVDEAHCISEWGHDFRPEYR
                     RIRPLIDEIGRAPIVALTATATPKVQHDIQKNLGMMDATVFKSSFNRPNLFYEVRAKT
                     SSIDKEIIKYIKANPGKSGIIYCLSRKKVEKLAETLQVNNIKALPYHAGMDSQTRTAN
                     QDAFLMERVDVIVATIAFGMGIDKSDVRFVIHYDIPKSLEGYYQETGRAGRDDGEGYC
                     LAFYSNKDLQKLDKFMQGKPVAEQEIGKELLLETAAYAESSVCRRKSLLHYFGETYDK
                     ENCGNCDNCINPKKQVEAQELLSMVLETVVAVKEKFKTDHIIDVLSGNETSDVVSYKH
                     NELELFGMGAEEDEKIWNAVIRQALIDGYLDKEIENYGLLKITDSGRNFLKKPRSFKV
                     TEQLDYEEMEEDMQMHGSATSAVDPIMFSMLKNLRKKISKQLNLPPYVIFHDPSLEAM
                     ATTYPITMEELQNIPGVGAGKAKRYGQEFLSLIKIHVEENEIERPEDLRVRTVANKSK
                     LKVSIIQAIDRKVALDDLANTKGLGFSELLDEIEAIVYSGTRINISYFLMEIMDEEHI
                     EEIFDYFKDSSSDSIQEAMDVLGGDYTEEEIRLVRIKFISEYGN"
     gene            9343..11265
                     /locus_tag="PLI37_03220"
     CDS             9343..11265
                     /locus_tag="PLI37_03220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ58060.1"
                     /translation="MRKILLACLLSVIFSNVRAQADDPVVMNINGENILRSEFEYNWK
                     KNYNISSTDKKNLDEYLDLFINFKLKVAEAKAQGIDTTQAFITELRGYRSQLTAPYLT
                     DKQTEEKLIDEAYAFTSQYVDLSHILIPLDRNAAPQDTLTVYNQLMELRERILKGEDF
                     GRLAAEYSQDNSSRDGGRIGTFLCSRLIYPIAKTAFYSQPGEIGLPVRTAFGYHLIKL
                     NAKHQAPGQYISGHILQSAPSGAPEEVADSAKQHITEIWGLLESGQDFAQLADTRNDD
                     RYVVGKGGKYQELSPGSLPYEYEQQVFDLKDGEYSQPFKTEYGWHIVKRFEITPLPEK
                     GEILEELKITLQRDERAQAGRRALVNKLKKDYNYQPDTKALTQFEQTLAGLENPDSTL
                     RTEAARLPVLASFEQNSITSTDFAEYYLQRKSLITSVPTAFEDYVEQRILAYEDSRLE
                     KKYPDFGNLMREYHDGILLFEISNQKVWEKAVEDQEGLAAYFKANKADYQWDKPRFKG
                     ALIHCADETTAKKAKKMAGKLKYDSLAFELKRSFNNDSTTLIKVEQGIYVMGEYAPVD
                     KLVFKSGDWNPDKSFPIVFTKGKVLKKGPEEYSDVRGAVIGDYQNYLEKEWIKELKQK
                     YPVSIFEEHLQILKAE"
     gene            11316..12215
                     /locus_tag="PLI37_03225"
     CDS             11316..12215
                     /locus_tag="PLI37_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ58061.1"
                     /translation="MKHLLWLLLLMPAWLQTCLGKETESPRVAVVRVSDATLYLDEIQ
                     NALPTGLNSVDSMRFVNSYTENWVKETLLYQAASKNLGQSPEINRMVENYRRSLIIYQ
                     YQQQALNEKMESEVTEADMAEYYEKNANRFISNKNLIKGLFIKVPLSAPGLDKLKIWY
                     KKNDTESLDNIEKYSYQNASIYEYFHETWTSLDDVMDQIPANIADQEKFLRNNKFLEV
                     EDGNYCYLLYISDYITTGSPQPLDYVSAQIRNILVNSRKTEFIHQFENDMLEQAKRKG
                     AIKYFSIPVVDTASAQDLTIDPQ"
     gene            12212..13561
                     /locus_tag="PLI37_03230"
     CDS             12212..13561
                     /locus_tag="PLI37_03230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ58062.1"
                     /translation="MKQFIYLIALILAFALQSLQAQNNIVDEIVWIVGDEAILKSEVE
                     AYRLQALQEGQKFSGDPYCVIPEQLAVQKLFLHQAELDSIIVTDNDVITNVDRNINRM
                     ITVLGSKEKMEEYFGKPISKIREEQREYARNEQTIHLMKQSLLKGLSVTPAEVRAYYK
                     SLPSDSIAIIPAEIEVEIITVEPRIPEAEIESIKERLREFTERVNNGERFSTLAVLYS
                     QHEATAKFGGETGFYGKGELMPEFAAVAFSLTDPTKVSRIVETDDGFHIIQLIEKRGD
                     RVNCRHILLRPEVSATERNEAMVRLDSIADFIRKGKLSFADAAMFYSQDKNTRKNGGL
                     MFNPNTGTAHFQLEELPQEVSKVVYSMNIGEISAPFSMRNPDNDREICAIVRVKNRTK
                     AHKATVEEDFVKLKDIVLQNKQEKLIREWIVNKQKGIYVRIDERWRNCDFQYPGWIK"
     gene            13602..15197
                     /locus_tag="PLI37_03235"
     CDS             13602..15197
                     /locus_tag="PLI37_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOQ58063.1"
                     /translation="MSLGLLCLCSLVLQAQGNRIFLEHANSTNFDKNISPDYHFLIGD
                     VQFRHDSARLYCDTAHYYRADNSLRAFGNVRIEQGDTLFIYGKTLFYDGNKKLAQLRN
                     NVRMVNKEVTLFTDHLDYDRVANIGYYYRGGKIVDASNTLSSDYGRYNPDTKMAFFKK
                     DVVLTHPQFLLKTDTLNYNTETGTASIISRTEIEAENADIIAYRGWYNTKTDKSLLLD
                     RSYIISEKRSLTADSILYEQSLDIGEAFVNIELIDSSRNTTLTSDYAFYDQSNNYVLL
                     TKKALLREYSGQDTFYLHADTLLGKQDSIYDTFRAYYNVRSYRRDLQGLCDSAFYSTR
                     DSVLQLFGNPILWADNQQISGQQIFLYTKNQQPDYMEVEGLALLASKEDSGLFNQSSG
                     RKLTAYFLDNKVRRVVIEGNPESIYLPRDEKQKILGMNRLENGSLYIFMDKEGKVERL
                     KVSPQPKGKFYPLQLLTEEVKYLEHFSWPIHLRPTDPADVFRLTDRPRAENMSTNIEG
                     AAGEGANAPGKAALRTFKSKNSK"
     gene            15199..15498
                     /locus_tag="PLI37_03240"
     CDS             15199..15498
                     /locus_tag="PLI37_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58064.1"
                     /translation="MSFFKQRKPRKFDHQPIYYDAQKEARQERLQKVRVELGLMDEKE
                     LKKDYEKIIRGSFKDAVPGGSRTKRKQDSNKTMRILVVLFIIILVVYFLASRYAG"
     gene            15488..17437
                     /gene="mutL"
                     /locus_tag="PLI37_03245"
     CDS             15488..17437
                     /gene="mutL"
                     /locus_tag="PLI37_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOQ58065.1"
                     /translation="MLDKNLAGNTIRVLPESVANQIAAGEVIQRPASAVKELMENAID
                     AGASQIDVLVKDAGKSLIQVTDNGKGMSPQDAILAFERHATSKLSVATDLLKIRSMGF
                     RGEALASIASVAQVELQSKRPEDELGTKVSIAGSKIENQETVLCASGSRFSVRNLFYN
                     LPARRKFLGSNAKEWLHVRNEFIQIALAYPELQLSLVHNGESIFRLSPSGLRQRIVAI
                     YGEQLNKKLFPIEVETEIVRISGFVGSPDAARKRASEQFFFVNRRFIRHPYFRKSILS
                     VFEPLVPPSYQPIFFVYLEAAPDSIDVNIHPTKTEVKFEHEQIIWPILNAAVRQSLGK
                     FHAVPSLDFDKENAPEIRVFTGEKDVPPPKVDFDPSYNPFQRKSDSVHKAGISFPDRD
                     KQIGHWEQLYRDFKAEGKSAVELPQTESGPVSKIQEEKESYLKEELDLLFDENSQIEA
                     LQTLFSASETLDEIGSNLFQVFGRYIALVTADALLLIDQHRAQTRILFEQYSNQLDTG
                     QSFSQTLLFPEILALDKNRLIEFESIKPDLISLGFQFEKNGENYLITATPEHTQGLHP
                     VAIVTDLLDASMESLPNFSNELKDKLALRLAKSTAIPYGQKLSLTEMKNLCQHFFDCA
                     IRNHTPEGKKIILRVSAQEIIKRLN"
     gene            17443..19182
                     /gene="rnr"
                     /locus_tag="PLI37_03250"
     CDS             17443..19182
                     /gene="rnr"
                     /locus_tag="PLI37_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOQ58066.1"
                     /translation="MAKKKTNVRIRKKDLKSDIIRFFQENRQKKYDYLQLAGTFNLKR
                     QTQQLMLFDILEELHRQGFLDKAAGNRYSTFKQKLTNNGTHPDSYESILEEFDISEDY
                     PEKALQAAELLSDSIPGEEYAYREDFRTVCTFTIDPWDAKDFDDALSLRKLPNGNWEV
                     GVHIADVTYYVKQGSAIDREAEKRGTSIYLVDRTIPMLPERLSNGLCSLRPDEDKRCF
                     SCIFEMNEAAQVKNHRIVRTVIRSDRRLNYEEALDIIQGKNDPVQEEIRVLNELAKKL
                     QSKRFKNGAIRFESSEICFKVDEKGKPLEIHFKTPTEANNLIEEFMLLANKTVAEFIG
                     KKSKKTFVYRIHDSPDLEKLEDVARFIRRFGHNLKLQGGKKAVAASINQLLGEVQGRK
                     EGDVISAITIRAMAKAVYSTDNIGHYGLAFDHYTHFTSPIRRYPDMMVHRLLERYMSG
                     GNSVNKQKWEEKCRHCSDMEMNAAQAERASVKYKQIEFMSERIGNSYEGVISGITDFG
                     FFVEIIENKCEGLVPMRDLNDDYYEFDEQNYCLRGRREKKVYRLGDPVKVQVLRANLE
                     KRQLDFKLINLEE"
     gene            19463..>19707
                     /locus_tag="PLI37_03255"
     CDS             19463..>19707
                     /locus_tag="PLI37_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOQ58067.1"
                     /translation="MNELFSSLPYKVADINLADFGRKEIKLAEHEMPGLMAIRRKYGE
                     SKPLKGARITGSLHMTIQTAVLIETLTALGAEVRWAS"
BASE COUNT         6064 a         4520 c         4301 g         4722 t
ORIGIN      
        1 agaccggccg ggaagatgtt gttttcttcg aaaacggctc ttttccgctt taaagcttcg
       61 gcagaatcgg cacaacacag cggcaactgt tctaaagact gcatcttttc ccgatgttcc
      121 ggcttgaaaa tgtttacgtc cacataaagt tgctcggcca gctcaagagc atccggccgc
      181 ctgattccat gaagagaagc aacaatcaaa gcggccaggg tcaggaaaat atcggccgaa
      241 ccgtcggaca ccctatactc aaaggtttgc ctggattccg aagcaggtag aggctccgat
      301 tccaagggat tggcatcaaa aatcattttt tcggcccctg tccagcccag aggcacgcgt
      361 accaccaccg agcggttgcg gtcgccccag caaatacggg taggtgcttc ctgattgggc
      421 accaatctga aataagaagt aggaatggtg tttccgaaag ctgtaatagc gtcgctcaaa
      481 tccaaaatgc cggctatcat cttgcgggca acatcgctta atcctccctt gcctgcaaca
      541 aggtttttac cgtctttttc gagcatcata tgaaaatgca ttccgctgcc ggctttgcct
      601 tcggtaatct tgggagcata actgatgcct acgccgtatt tgttgcccaa cattctcagt
      661 atccatttgg ccaacaccaa ttgttcggcg gctttttcaa cggtacaggg taagaattcg
      721 atttcatgct gctcgtaata gctttctccc tgagtaaaac aacccacctc ggaatgtccg
      781 tattttatgt tgccacccgc catggctatc atttgcaaag cctcgttacg aataaactcg
      841 tatttggcaa aaggttcgct ggcgtgataa cctttttgat ctacaccgga atacaagcct
      901 ccggccggag caatcacata atattccaac tcgcccagtg ccataaagcg actgccggtt
      961 tctttggtaa acaattgatc ggctctgtgt aaaatcgtat acggatcgct ttcgagaggc
     1021 ctgccgtggt tgttgtaaaa agaacagaaa atgtccagag tgggaacctc tgcaaaggga
     1081 tttacaaaag ccgtgcgata acggggaacc acataaagat cgctgctgcc cgattcgatg
     1141 aaagaaaata aactgctgcc gtctacccgt tctccccggg tcagtatcgt ttcgaggtag
     1201 tccctggaat tgattacaaa attcaaggtt ttcaaacgac cgtcttcggc cacatagcga
     1261 aaattcagca ttttaatctg ccgttcttcg caaaaacgca taatatcctt gcgggtaaag
     1321 tcttgtgaag gtttgtttaa aaattgaacc agttcattag ggttcatcga gattccggaa
     1381 tctttcatcg aacgagattg aattaaaaag tgcaaaattc atcctcttgc gcaagaaaaa
     1441 caaatcttgt ggctttattt ttagaattat ttcaaaaaat ccatgttcat agcaaattct
     1501 agtgcaaaat cttcgtcgtc ggacagttct atgtattgcg attgttgaac aaccctgtgc
     1561 actttgttgg caaacacttc tgagcgaaga aactgcaagg cgccgatgcc ggcactgttg
     1621 cctaccgcga aggttttatc tttgagttcg gccgggagca agcctatttc aacggccgag
     1681 ggtatatgaa tgtaattgcc caggcctccg gccagaaaca aggcttccaa ctcctgaaaa
     1741 ttcaagccgg cacgctttaa taaaatttta atgcctgaat aaatggccga tttagccagt
     1801 tgaatttccc ggatatcggc ctgagttatc tgtatgccgg ggtaaatttc aaaatcctgc
     1861 tgaatcaatc cgctttcgtc cattaaatga ttacgccgca accaggcgat cacatcgatg
     1921 gctccggagc cgcaaacacc tacgggctcg atgtctccaa tgactttgaa atttgaaggg
     1981 ctttcaaaag cggcaatggc tcccttaagt gcgggcatcc cggagctcag tccggctcct
     2041 tcgaaagcgg gaccggcggc cgttgcacaa gtaaatattt cctccccttt gaccaaagcc
     2101 atttctccgt tggttccgat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 tagctgatcc tggaaatcac atcggcaccg taggctgttt gcggattcat ccaggaactg
     2281 atcttcacaa ttcgcccgct ggctaaatgt acgaaataac acactatagt ggtggtaccc
     2341 aagtctatgg ccgccccata agcgtcggga tgcaacaatt gccggctgtg tgcgggtgga
     2401 aaatcctcga gataagctgt ttgagtggca agtatctgcg cttcttcttt ggcaggcaaa
     2461 atcacttcaa tatcaccggc agggtaataa ttgcaggcca gcactttccc ctctcctttg
     2521 atatcgacca aacatttacc gcaacggcct ttccctccgc agggagcata gatggaataa
     2581 tcttcgttgc ggagaatttc cagcaggctc tgttggccct gagtttgaat tacccttgtt
     2641 tgagagtttt ctctcaacag aattttatac atgttgcttt tttaatgtcg attttctgta
     2701 acgcaggtaa aaaaacaggc cacatgccgt caacagcagt atagatggaa taagttgcca
     2761 ggtattttcc cctacaccca acagctcggg gtctgatgcc aggtaattgt ttttttcggc
     2821 gtaatgaaac actacggaaa tagcgttgtt ggcaaaatga gccgccacag gaacccagat
     2881 cgatccggac cataaaagca ggtagccaaa ccaggccccc aacaatagac gaggtataaa
     2941 accgtaaaat tgcatatgta tcagactgaa gacgaaagca gttatccaga tggccaactg
     3001 aattctgccc ttagaagaaa tgatactttg caaagtcccc ctgaaaagta attcttctcc
     3061 aaaacccgcc ataacagcta ctaccagcaa ctgccacaat aaatcgctcc aggattctcc
     3121 ttgcagcatc aattccgtga cgcgttgtgc attgtcttcg gcaaccctca tccatttttc
     3181 tacaccctct aaaaaggagg gaaactgtat gtcctgattc caacttacca agaaattaac
     3241 cagcggtacg gcagctaaaa tactgaacat ggccaataaa acatcaacgg gtttggcttt
     3301 gaccaatttc aaaaaacggc tgtttttttc accgtaccaa taacttaata gaaaaggtgg
     3361 aagaatgaac accaaaaggg tttgcaggcc ctgcatcaac aaaagaaaag aagggttgct
     3421 catttgtgcg cccaactcgg aaccaagtcg cccaaagctc aaaccacggc tgagtagtaa
     3481 aaaaaggttg agtagtaagg agcttaaaaa gagagcaatt atcactgtgg ctatcagcac
     3541 tataagcctt aagcccattt tgcttctgtc tatctttgtc attcaatgag cattttttcg
     3601 caaacctaat tcaaagtttt gtcaattcaa agattaagag tatttttgcg tctccaaaaa
     3661 aatgccttta agagcaacaa gcagaaggac aaagccctgc tttgaaaagc ttgttcggta
     3721 aaaggacagg ttcaaacatc aacaacatga atgtagaaaa acaactcatt gatccggtca
     3781 atgcaagtat caagatcaac ttagtcaaag ccgactatca ggaaaaagta gataaaacac
     3841 tcaaaaccta tcgccaaaag gccaatgtgc ccggtttccg tccgggaatg gtgcccttgg
     3901 gtctgattca gaaaatgtac ggaaaagccg ttctggcaga agaaatcaat aaaattgtat
     3961 tggaagcttt atacaattac atcaaagaaa ataatctgaa catcttgggc gaacccctac
     4021 ccggcaaaca acaaacccct ccggatttca atgccggcga agattttgaa tttatttttg
     4081 atattgccct ggctcccgaa cttgatttta caataagcaa aaaagacagc atcccttact
     4141 ataaaataga ggtcagcgac gacatgctgg agaaacagat taaatcgctg gcaagtcgcg
     4201 gcggaaggta cgaaaaagtg gaagaggccc aggaaggcga caccatcaag ggaacactga
     4261 ccgagattcc cgaagaaggt caaaaagatt cagaagatct tgtaaaagtg gaagaagcca
     4321 ttattcttcc tgcctatttc aaaaatgaag aagaaaaggc caaaatgaaa ggtgttcgtc
     4381 ccggcgacaa aatcagattt aatccttata aagccgccga aggccacgaa acggaattgg
     4441 cttccatgct gcatttgccc aaagaaaaag ccgtggaaat caagtccgat tttgaattcg
     4501 aagtaaaaga aatcagccgt ttcaaagaag ccgaactcaa ccgggaactc ttcgaccaga
     4561 tttacggtaa agacgttatt acctccgaag aagccttccg taccaaagtt agggaagaaa
     4621 tagaggcccg gttcgcaccc gaaagcgatt atcgttttat ggtagatgcc gaaaaacaat
     4681 tggtaaagaa actcaaggac gtggaatttc ctgttgaatt cctgaaacgc tggctcttgg
     4741 ctgccgacga aaaacgcaat cccgcaacca tagatcagga catgcccaaa cttgtcgaag
     4801 aactgaagtg gcatctgatg aaagaagaaa tcatcaaacg caacaatatt caggtaagcg
     4861 aagccgatgt cctggaaacc gccaaaaaag caacccgggc acaatttgcc caatacggca
     4921 tgatgtccgt cccggatgat ttgttgaaca actacgctgc cgaaatgctc aaaaaacccg
     4981 agacggcaag gaacctgagc gaccagacca acagcgccaa ggtcgccgac tttttaaaat
     5041 ctgctgtcaa gttgaacgaa aagactgtat cagccgaaga attcaataaa ctttacgaaa
     5101 attgaaaaac atgaatgatt ttagaaaata cgccacccgc catttaagaa tggacggcat
     5161 ggctttagac caatattcaa aaattaccag tagctatatt tctccaacca ttatcgaaga
     5221 gagacaattg aatgtagctc aaatggatgt tttttcgcgc ctgatgatgg accgcatcat
     5281 tttcctgggc actcaagtag acgattatac agccaacgtc gtgcaagctc agctgctcta
     5341 cctggattct gccgaaccgg gcaaagacat ttctttgtac ctgaattctc cggggggaac
     5401 tgtttatgcc gggctgggca tttacgacac catgcagttt atttccagcg acgtttctac
     5461 catttgtacg ggagtggcag cctcgatggc cgccgtactt ttggtagccg gcaccaaagg
     5521 caaacgcttt gcacttaaac attccagggt aatgatacat caaccgatgg gtggagttca
     5581 gggacaagcc tccgatatgg aaatcacggt aagagaaatt cagaagatta aaaaggaatt
     5641 gtattctatt atctcatcgc attccggaca aaccattgaa caaatcgaaa aagattccga
     5701 tcgcgactat tggatgactg ccgccgaagc taaagactac ggaatgattg acgaggtgat
     5761 gttgaagagc aaataataat ggctaaaaca tctacggata agtgcagttt ttgcggcaga
     5821 tcacgcagcg aggttgaact tttgatttcc ggcatttcgg ctcatatttg tgaagaatgc
     5881 gcagaaagag ctcacagcat aagcaaagaa attatcggct catcaaagaa taaacagttc
     5941 gagcttaagg ctgaagattt gcccaaaccc aaagagatca aggctttttt ggatctatac
     6001 gttatcggcc aggaagaagc caaaaagttc ctatccgttt cggtttacaa ccattataaa
     6061 aggttgatgc agacaaaaaa gaacgatgag gtagagatag agaaatcaaa catcatcatg
     6121 gtaggggcca ccggcacggg caaaaccttg ctggcaaaaa ccatagccaa aaaactgaaa
     6181 gtgcctttta ctatagtgga tgccacggtg cttaccgaag ccggttacgt gggcgaagac
     6241 atcgaaagcc tgttgacacg cctgcttcag gtggccgatt acgatgtggc tgctgccgaa
     6301 aaaggcataa tttttatcga tgaaatagac aagattgccc gcaagggaga taatccttcc
     6361 atcacacgtg acgtcagcgg cgaaggagtg caacagggct tgctgaagct gctcgaaggt
     6421 tctgtggtga atgtcccgcc tcagggaggg cgtaaacatc ccgaacaaaa aatgattccc
     6481 gtcaacactc aacatatatt gtttatctgc ggcggcgctt ttgacggtat agaaaaaaag
     6541 atagccgctc gcttaaacac ccaggtggtc ggttttggag cctccaagca agttgcccgg
     6601 attgatcgtg aaaacttact gcaatacatt gcgccacagg atctcaaggc ctttggaatg
     6661 atcccggaaa tcatcggcag gctgcctgta cttacctatc tggaagccct ggatcgtaag
     6721 gcattacgag ccattcttac agaaccgaag aattccatcg ttaagcagta tattaagctt
     6781 ttcgaaatgg acggggtaac tttgagcttt gacgatgatg tccttgaata cattgtagac
     6841 aaggctattg aatttaagct tggagcaaga ggtttgcgtt ccatcgccga aaccatcatg
     6901 atggatatga tgtacgaaac tccgtccggg cgcagaaaaa atatccgtat agatttaaaa
     6961 tacgctcaat ccaaaattga ggcagctaac cagtccaggc tcagggaaca atagcatgtt
     7021 tcagaaaaat aattctagtt tttcccttta tttgttggta ttctgaaaga tttgttttta
     7081 aatttgtttg tcagcaaaaa aacgtagtta tggcaaaatc caatcctttg gcagaaagtc
     7141 ttaagttgca ttttggattc gacactttca aaggaaatca agaagctatc atccggaaca
     7201 taatggaagg caaggacact ttcgtgctca tgcctaccgg gggtggaaaa tctttgtgtt
     7261 atcaattgcc ttctctgctt ttaaatggca cagcaatagt tatttcccct cttatcgcac
     7321 tgatgaagaa tcaggtggat gccatgcgta atttcagcga cgaagacgga gtggctcatt
     7381 ttattaattc ttccttgaac aaagccgcca tcgatcaggt acgcaacgat attacgtcgg
     7441 gcaaaaccaa attgctctac gtggctcccg aatctctgac caaagaagaa aacatcgatt
     7501 ttctaaaaca gatccccatt tcgctctttg cggtagacga agcgcattgt atctcggagt
     7561 ggggtcatga cttcaggccg gaataccggc gtatccgccc acttatcgat gaaatcggca
     7621 gagcacctat cgtagcgctt acagccaccg ccacgcctaa ggtgcaacac gatattcaaa
     7681 aaaacctggg aatgatggat gccactgttt ttaaatcatc cttcaaccgc cccaatcttt
     7741 tttacgaagt cagagccaaa acctccagca tcgataaaga aatcattaaa tacatcaagg
     7801 ccaatcccgg caaatcgggt attatttatt gcctgagccg caaaaaggtg gagaaactgg
     7861 ccgaaaccct gcaagtaaac aatatcaaag ccctccctta tcacgccggt atggattcac
     7921 aaacccgaac agccaaccaa gatgcgtttc tgatggaaag ggtagatgtt atcgtagcga
     7981 caattgcctt tgggatgggt atcgacaaat cggacgtacg ctttgtcatt cattacgata
     8041 ttcctaaaag tctcgaaggt tattatcagg aaacagggcg agccggacgc gacgacggcg
     8101 aagggtattg tttggctttt tattccaaca aggatctgca aaaactcgat aaatttatgc
     8161 agggcaaacc tgtggccgaa caggaaatag gtaaggagct tttactggaa actgcggctt
     8221 atgccgagtc atcggtgtgc agaaggaaat ctttactcca ttatttcgga gaaacttacg
     8281 ataaagaaaa ctgcggtaat tgcgacaatt gcataaatcc aaaaaaacaa gtggaagctc
     8341 aggaattact ttctatggtt ttggaaaccg tcgttgctgt caaagaaaaa ttcaaaacag
     8401 atcacattat tgatgtttta tcgggcaacg aaacctcgga cgttgtttcc tataaacaca
     8461 acgaactgga attgttcggc atgggagccg aagaggatga aaaaatctgg aatgccgtta
     8521 ttcgtcaagc ccttatcgat ggctatctgg ataaggaaat cgaaaattac ggcttactca
     8581 agattaccga ctcgggcaga aattttctga aaaagccgcg cagcttcaag gtaaccgagc
     8641 aattggatta cgaggaaatg gaagaagata tgcaaatgca cggcagtgca accagcgccg
     8701 tagaccccat catgttttcc atgctcaaaa atttgcgcaa aaaaatatcc aagcaactca
     8761 atcttccgcc ttacgtcatt tttcatgatc cttccctgga agccatggcc accacctacc
     8821 ccatcacaat ggaagagttg caaaatattc ccggagtagg ggccggcaag gccaaacgtt
     8881 acggacagga atttctttcg ctcatcaaaa ttcacgtaga agaaaacgaa atcgaacgtc
     8941 ccgaagattt gcgcgtgcgc accgtagcca ataaatcaaa actgaaagta tccattattc
     9001 aggctatcga ccgcaaggtg gcgctggacg acctggccaa taccaaaggc ctgggttttt
     9061 cggaattact cgacgaaatc gaagcaatcg tatactcagg caccagaata aacatctctt
     9121 acttcttgat ggagataatg gacgaagaac acatcgaaga gattttcgat tacttcaaag
     9181 attcatccag cgacagcatc caggaagcta tggatgtgct gggtggcgat tataccgaag
     9241 aagaaattcg cctggtccgt attaaattta tttctgaata cggaaactaa acaacgtatt
     9301 tacgcgtttg aatggtaatg ctcacattat actgattgtc ttatgagaaa aattttgtta
     9361 gcctgtctgt tatctgttat tttttcaaat gtccgagctc aggccgatga cccggtagta
     9421 atgaacatca acggggaaaa tatactcaga tcggaatttg aatacaactg gaaaaaaaat
     9481 tacaacatca gttctacgga taaaaaaaat ctggacgaat acctggatct cttcataaac
     9541 tttaagctta aagtggccga agccaaagct cagggaatag acaccactca agcctttata
     9601 accgaactca ggggctaccg tagtcaattg acggctccct atcttaccga taaacagacc
     9661 gaagaaaaat taatagacga agcttatgcc tttacgagtc aatacgttga tttgagtcat
     9721 atcctgattc cgctggacag aaatgcagct ccgcaagata ctctgacagt gtacaaccaa
     9781 ctcatggagt taagggaacg cattttaaag ggagaagatt ttggccgcct ggctgcagag
     9841 tattctcaag ataattcgag ccgcgacgga ggccgcatcg gaactttctt gtgttccagg
     9901 ctaatctatc ccatcgctaa aaccgccttc tacagccaac cgggagaaat aggcctgccc
     9961 gtcaggactg ccttcggcta tcatctgata aaactgaatg ccaaacacca ggctcccgga
    10021 caatacatct cgggacatat tcttcaatct gcacccagcg gtgctcccga ggaggtggca
    10081 gattcagcca aacaacatat taccgaaatc tggggcctgc tcgaaagcgg acaagatttt
    10141 gcccagttgg ccgacactcg caacgacgac cgctatgtgg tgggtaaagg aggaaaatac
    10201 caggaactaa gcccgggatc gctgccctat gaatacgaac aacaggtgtt cgatttaaaa
    10261 gacggcgagt attcccaacc ttttaaaacc gaatacggct ggcatattgt aaaacgcttt
    10321 gaaatcactc ccctacccga aaaaggagaa atccttgaag aactcaagat cactttacaa
    10381 cgcgatgaaa gagctcaggc aggcaggagg gccctggtaa acaaattaaa aaaggattat
    10441 aattatcaac cggacaccaa ggctctgact caatttgaac aaaccctggc cggactcgaa
    10501 aatccggact ctacgttgcg gacagaagca gcccgactgc ccgtgctggc aagtttcgaa
    10561 caaaactcca taacgtccac ggattttgct gaatactatt tgcaaagaaa atccctgatc
    10621 acctccgttc ctacggcttt tgaagactac gtcgaacaga ggatactggc ctatgaagac
    10681 agccggcttg aaaagaaata cccggatttt ggaaatctga tgcgtgaata ccacgatggc
    10741 attctgcttt ttgaaatcag caatcaaaag gtttgggaaa aagcagtcga agatcaggaa
    10801 ggcctggcgg cttacttcaa agccaacaaa gccgactatc aatgggacaa acccaggttc
    10861 aaaggagccc ttatccattg tgccgacgag accaccgcca aaaaggccaa aaaaatggcc
    10921 gggaaattga agtatgattc actggctttt gaattgaaac gcagcttcaa taacgacagc
    10981 accactctga tcaaggtgga acaagggatt tacgtgatgg gagaatatgc cccggtagat
    11041 aaattggttt tcaaaagcgg cgattggaat ccggacaaat cgttcccgat cgtatttacc
    11101 aagggtaaag tcctgaaaaa aggacccgaa gaatattccg atgtacgcgg agcagtcatc
    11161 ggtgattacc aaaactacct ggaaaaggaa tggattaaag aactcaaaca aaaatatccc
    11221 gtcagtatct tcgaagaaca cctccagatc ctaaaagcag aataaacaaa tactgagggt
    11281 tttttagtat ctttgtactc tttttatgtt agactatgaa acatctactc tggctgttgt
    11341 tgcttatgcc tgcctggctg cagacatgcc tgggcaaaga aaccgaatcg cccagagttg
    11401 ccgttgtccg ggtctcggac gctacccttt accttgacga aattcaaaac gctctgccaa
    11461 caggcctgaa ttcagtcgac agcatgcgtt ttgtcaattc ttacaccgaa aactgggtaa
    11521 aagaaaccct tttataccaa gctgccagta aaaatctggg ccaaagtcct gaaataaaca
    11581 gaatggtaga aaactaccgc cgctcgctca ttatatatca atatcagcaa caggcgctga
    11641 acgaaaaaat ggaatcggag gtgaccgaag ccgatatggc agagtattac gaaaaaaacg
    11701 ccaatcgttt tatttccaac aaaaatctga ttaaaggatt gtttatcaaa gttcccctgt
    11761 cggctccggg actggacaaa ttgaaaatct ggtacaaaaa aaacgatacc gaatcgctgg
    11821 acaatattga gaaatattcg tatcaaaacg caagtattta tgaatatttc cacgagacct
    11881 ggactagtct ggacgatgta atggatcaga ttccggccaa tatagccgac caggaaaaat
    11941 ttctccgcaa caataagttt ctcgaagtcg aagacggaaa ttactgttac cttttgtata
    12001 taagcgatta cattacaacc ggcagccctc agcccttgga ctacgtgagc gcccaaataa
    12061 gaaacatttt agtcaacagc aggaaaacag aattcattca tcaatttgag aacgatatgc
    12121 ttgaacaagc caaacgcaag ggggcaataa aatacttttc cattcctgtt gtcgacaccg
    12181 cctccgctca agatttaacc atcgatccac aatgaaacag tttatatacc taatagccct
    12241 tatactggcc ttcgcattac aaagcttgca agcccaaaac aacatagttg atgaaattgt
    12301 ctggatagtg ggcgatgaag ctattctgaa atccgaagtg gaggcctatc gtctgcaggc
    12361 tttgcaggaa ggacaaaagt tcagcggcga tccctattgt gtcatacccg agcaactcgc
    12421 tgttcaaaaa ttatttctac atcaggctga actggacagt attatagtga ccgacaacga
    12481 tgtaatcacc aatgtggacc gcaacatcaa ccgtatgatt acggtactgg gttccaaaga
    12541 aaaaatggaa gaatattttg gtaaacctat ttccaaaatc cgcgaggagc aaagggaata
    12601 cgccagaaat gaacaaacca tacatctgat gaagcaatcc ctgctcaaag gtctgagcgt
    12661 tactcctgcc gaagtgcgtg cttattataa atcgctgccc tccgacagta ttgccatcat
    12721 cccggccgaa atcgaagtgg agattatcac agtggaaccc agaattccgg aggctgaaat
    12781 tgaatccatc aaagaacgcc tgcgtgaatt taccgaaaga gtcaacaacg gcgaaagatt
    12841 ttctaccctg gctgttttgt actctcagca cgaagctacg gctaaattcg gaggagaaac
    12901 cggtttttac ggtaaaggag aactgatgcc tgaatttgcc gccgtcgcct ttagtctgac
    12961 agaccccacc aaagtgtcgc gcattgtgga aaccgacgac ggttttcata tcattcaatt
    13021 gatcgagaaa agaggagatc gcgtgaattg ccggcatatt ttgcttagac ccgaggtttc
    13081 agctaccgaa agaaacgaag caatggtacg tctggatagt attgccgatt ttattcgcaa
    13141 gggtaaatta agtttcgcgg atgcagccat gttttattcg caagacaaga acacccggaa
    13201 aaacggagga ttgatgttca atcccaatac ggggaccgct cattttcaac tcgaagaatt
    13261 gcctcaagaa gtcagcaagg tggtttactc gatgaatata ggcgaaattt ctgctccctt
    13321 tagtatgcgc aatcccgaca acgacaggga aatatgcgcc atagtgcggg tcaaaaacag
    13381 gaccaaggct cataaagcaa cggtagaaga agatttcgtg aaattaaaag atattgtctt
    13441 acaaaataaa caagaaaagc tcatccggga atggatcgta aacaaacaaa aggggattta
    13501 tgttcgtatc gacgaaagat ggcggaactg cgatttccaa tatccgggat ggattaagta
    13561 atatgttcaa tgttgccgac ataaagcctg cattgcggca tatgagcctt ggtttattgt
    13621 gcctctgttc tcttgttttg caggctcagg ggaacagaat attccttgaa cacgccaaca
    13681 gcaccaattt tgacaagaac atcagtcccg actatcattt tttgataggc gatgtacaat
    13741 tcaggcacga cagtgcccgt ctgtattgcg atacggctca ttattacaga gccgataatt
    13801 ctctccgggc ttttggaaat gtccgaattg aacagggcga taccctgttt atttacggta
    13861 aaaccctttt ttacgacggg aataaaaaac tggcccaact ccggaataat gttcgcatgg
    13921 tcaacaaaga ggtgacctta tttaccgacc acctggatta cgacagagtg gctaacattg
    13981 gatattacta caggggcgga aaaatcgtcg atgcttccaa tacccttagc tcagattacg
    14041 gacgctacaa ccccgacacc aaaatggctt ttttcaaaaa ggatgttgtt ctgactcacc
    14101 ctcagtttct gttgaaaacc gacactttaa attataacac cgaaacagga acggcctcca
    14161 ttatttccag gaccgaaatc gaggccgaaa atgcagatat aatagcctac cggggctggt
    14221 acaataccaa aaccgataaa agcctcttgt tggatcgttc ctatattatc agtgaaaaac
    14281 gcagtctaac tgcagattcc attctttacg aacagtcgct cgacatcggt gaagcctttg
    14341 tgaacattga attgatagat tcttcgagaa atactacgct aacttccgat tacgcctttt
    14401 acgatcaaag caacaattac gtcctcttaa ccaaaaaggc cctgctcaga gaatactccg
    14461 gccaggacac cttttatttg catgccgata ccctgctggg aaaacaagac tctatttacg
    14521 acaccttcag agcttattac aatgtcagga gctatcggag agatttacag ggactctgcg
    14581 attctgcctt ttattcaacg cgcgactccg tactccagct tttcggcaat cccatccttt
    14641 gggcggacaa tcagcaaatc agtggacaac agatctttct gtacaccaaa aatcaacaac
    14701 ccgactatat ggaggtggag ggcttggctt tgctggcatc caaagaagac agcggcctct
    14761 ttaatcaatc ctcgggcaga aagcttaccg cctattttct ggataacaag gtgcggcggg
    14821 tcgttataga aggcaatccg gaatccatct acctcccccg cgacgaaaaa caaaagatat
    14881 taggtatgaa ccgcctggaa aacggttctt tgtacatatt catggacaaa gagggtaaag
    14941 ttgaaagact aaaagtgagc cctcaaccta aaggaaagtt ttatcctttg caactgctca
    15001 ctgaggaagt gaagtatctt gagcatttca gctggcctat acacctgcgc ccaacagatc
    15061 ctgcggatgt ttttcgtctg accgacaggc caagagctga aaacatgagc accaacattg
    15121 aaggtgctgc cggcgagggt gcaaacgctc ccggcaaggc agcactcaga acatttaaaa
    15181 gtaaaaacag taagtaatat gtcgtttttt aaacaaagaa agcccagaaa atttgatcat
    15241 caacccattt attacgatgc tcaaaaagaa gcccgccagg agcgcttgca aaaagtcaga
    15301 gtagagctgg ggctgatgga tgaaaaagag ctaaaaaaag attatgaaaa aatcattcga
    15361 ggcagtttca aagacgctgt cccgggtgga agtcgcacca aacgaaaaca agacagcaac
    15421 aaaacgatga gaattcttgt cgtactgttc ataatcatcc tcgtggtata ttttttagct
    15481 tctcgttatg ctggataaaa acctggccgg caacactata cgggttttgc ccgagtcggt
    15541 ggctaaccaa attgccgccg gagaggtgat tcagcgtcct gcctccgctg taaaagagct
    15601 gatggaaaat gccatcgacg caggagccag ccagattgac gtgctggtaa aagatgccgg
    15661 aaagagcctg atccaggtaa cagacaacgg taagggcatg tcgccccagg atgcaatact
    15721 cgctttcgaa aggcatgcta cctccaaatt gagtgtggca actgacttac taaaaattcg
    15781 cagcatgggc tttcggggcg aagctctggc ttctatagcc tcggtagccc aggtggaatt
    15841 gcaaagcaaa agacctgaag acgaactggg aaccaaagta tcgatcgccg gctccaaaat
    15901 cgaaaatcaa gaaacagtgc tttgtgcttc aggctcacgc ttttcggtac gtaatctatt
    15961 ttacaattta ccggctcgtc gaaaatttct gggtagtaac gccaaagaat ggctgcacgt
    16021 acgtaatgaa ttcattcaaa ttgccctggc ctaccccgaa ttacaactca gccttgtcca
    16081 caatggcgaa agcattttca ggctatctcc ttcgggattg agacaacgca ttgttgctat
    16141 ttatggtgaa cagctcaaca aaaaattatt ccccatcgaa gtggaaacgg aaatcgttcg
    16201 aatcagcggt tttgtaggat cacccgatgc ggcacgcaaa agagcctccg agcagttttt
    16261 ctttgtaaac cggcgattta taagacaccc ctatttcaga aaatccattt tgagcgtatt
    16321 tgagcccctg gtgcctccgt cgtatcaacc gatctttttt gtttatcttg aagctgctcc
    16381 cgacagtatt gacgtgaaca tacatcccac aaaaaccgaa gtaaagtttg aacacgaaca
    16441 aatcatctgg cccatcttaa atgccgccgt acgtcaaagt ctgggaaaat ttcacgccgt
    16501 tccctcactg gacttcgaca aagaaaatgc tcccgaaatc cgggtgttca ccggagaaaa
    16561 agacgtgcct cctcccaaag tggactttga tcccagctac aatccttttc agcgcaaaag
    16621 cgacagcgtg cacaaagcgg ggatatcatt tccggacagg gacaaacaaa taggccattg
    16681 ggaacagttg tatcgcgatt tcaaagcgga aggtaaaagt gctgtagaat taccgcagac
    16741 agaatccggc ccggtaagca aaattcagga ggaaaaagaa tcttacctga aagaagaact
    16801 agaccttctt ttcgatgaaa actctcagat tgaggcctta caaaccctgt tttcagcctc
    16861 ggaaaccctg gacgaaatcg gttcaaatct ctttcaggtt ttcggtcgct atattgccct
    16921 ggttacggca gatgcacttt tgctgataga tcagcacaga gcccaaacca ggattttgtt
    16981 tgaacaatac agcaaccaac tcgacacggg gcaatctttt tcgcaaacct tgcttttccc
    17041 ggaaatcctc gcactggaca aaaaccggct gatcgaattt gagagcatca aacccgattt
    17101 aatcagtttg ggctttcaat tcgaaaaaaa cggcgaaaac tatctgataa cagccactcc
    17161 cgaacatact cagggtttac atccggtagc cattgtcacc gatttgctcg atgcctccat
    17221 ggagtcttta cccaactttt cgaatgaact gaaagataag ctggccctgc gtttggccaa
    17281 atcgaccgcc ataccctacg gacaaaaatt gagtcttaca gagatgaaga atttgtgcca
    17341 gcatttcttc gactgcgcca tcaggaatca tactcccgag ggcaaaaaaa tcatcctgcg
    17401 cgtgtcggcc caggaaataa taaaacgact aaactaagta aaatggccaa aaaaaagacc
    17461 aatgtccgaa tcagaaaaaa agacctgaaa tcggatatta tacggttttt tcaggaaaac
    17521 cggcaaaaaa aatacgacta cctgcaactg gccgggactt ttaatttgaa aagacaaacc
    17581 cagcaactga tgctcttcga tattttggaa gaactccaca ggcagggttt tttggataaa
    17641 gctgccggga atcgctattc aactttcaaa caaaaactca caaacaacgg tactcacccg
    17701 gatagctacg aaagtattct cgaggaattc gacattagcg aagattatcc tgaaaaagcg
    17761 ctgcaggccg ccgaattatt atcggatagt attcccggag aagagtatgc gtacagagaa
    17821 gatttcagga cagtctgcac ctttaccatc gatccctggg atgccaaaga tttcgacgac
    17881 gccctctccc ttagaaagtt acccaacggc aactgggaag tcggcgttca tattgccgat
    17941 gtcacttact acgtaaaaca aggctcagcc atcgaccggg aggccgaaaa aaggggtact
    18001 tcgatctact tggtcgaccg caccatccct atgctgcctg aacgcttgag taacgggcta
    18061 tgttcgctca gaccggatga agataaacgc tgtttttcct gcatctttga aatgaacgag
    18121 gccgctcagg ttaaaaatca ccgcattgtg cgcacggtaa tacgctctga caggcgcctg
    18181 aactacgaag aagccttaga cattattcag gggaaaaacg atcccgttca ggaagaaatc
    18241 cgtgttttaa acgaattagc caagaaactg caaagtaaac gcttcaaaaa cggagccatc
    18301 cgtttcgaga gttcagaaat ctgcttcaaa gtggacgaaa aagggaagcc cctggaaatt
    18361 catttcaaga cccccaccga agccaacaac ctgatcgagg aattcatgct tttggccaac
    18421 aaaacggtgg ccgaattcat tggtaaaaaa agcaaaaaaa cctttgtcta cagaattcac
    18481 gacagccccg atctggagaa gctcgaggat gtagcccgtt ttatcaggcg cttcgggcat
    18541 aatttaaaac tgcagggagg caaaaaggcc gtagcggctt ccatcaatca attgctgggc
    18601 gaggtacaag gccgcaaaga aggtgatgtt atttctgcca taaccatacg agccatggcc
    18661 aaagccgtct attccaccga caacatcgga cattacggac tggcttttga tcattacacc
    18721 cattttacct caccaatacg ccgttatccc gatatgatgg tgcaccgcct gctggaacgc
    18781 tatatgtcgg ggggaaacag cgtcaataaa cagaagtggg aggaaaaatg ccgccactgt
    18841 tccgatatgg aaatgaatgc agcccaggcc gaaagggctt cggttaaata caaacagatt
    18901 gaatttatgt ccgaacgcat cggcaatagt tatgaaggtg tgatttcggg tatcaccgat
    18961 ttcggctttt ttgtggaaat catcgaaaat aaatgcgaag gtctggtacc catgcgcgac
    19021 ctcaacgacg attactatga attcgacgaa caaaactact gcctcagggg aagacgcgaa
    19081 aaaaaggtct acaggctggg agatcctgtc aaggtgcagg tgttaagagc caatcttgaa
    19141 aaaaggcaat tggactttaa gttgataaac ctggaagaat aaaatgagta atttctttag
    19201 aaaaacaagc cgcctcaact cacgttgaga cggcttgcat atatagagga aattaaaaag
    19261 agttgtctat cacaatcgtt tcaagcacaa actcatcgat atagatgtat gctttgaaaa
    19321 aaacgctaca aaaaaaatgc attttttttc ttaaagttta atctcagcat aaaaaaccga
    19381 ttccccggct tttttttgta tctttgcaca ccaaaaacgt gttaataatt aatcgattaa
    19441 aaacatattc cccatttgca tcatgaatga gttgttttct tctcttcctt acaaagtagc
    19501 cgacattaat ctggccgatt ttggccgtaa agaaattaaa ctggcagagc acgaaatgcc
    19561 cggcctaatg gccatccgtc gtaaatacgg cgaaagcaaa cccctgaagg gcgcccgcat
    19621 taccggttcg ctacatatga ccatccaaac ggctgtattg atcgaaactc tcaccgccct
    19681 cggagcagaa gttcgctggg ccagttg
//
LOCUS       DAOSTD010000029        19691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_2929_length_19691_cov_15.031473, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000029 DAOSTD010000000
VERSION     DAOSTD010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19691
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2929_length_19691_cov_15.031473"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..6452)
                     /locus_tag="PLI37_03260"
     CDS             complement(<1..6452)
                     /locus_tag="PLI37_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58068.1"
                     /translation="MLLLSFPGRVESATYYLTASGAGSAQVASNWNTEPDGSGTAAGS
                     FTVSGDIFIIPPDIDGTVSANWIFNHIQNSVMTLEVNGSLSINQGVVMTLYRKNKGYT
                     TMIVRGSLIFKDSGINQLIGTYQGANNGETRFSLLPGATVKTPNTAGLLGTGTQSLRT
                     TNIIIALDNAANYYFNGSTNQTLSGLPTRVNDLVLEGSGTKTLASNLTVNGYLSLRQT
                     AYFANGGYTLSFGPASSLEYKNTGNRTTGTEFPANFGGAGGLIIDPGTGYTVSLSGNK
                     TALTNIFIRSGTFDMQTYTLSRATSGGTLSIEAGTTLRIGGTGSISANYSVPILDCTS
                     NIEYYGSNQIVRGINYGNLLLSGSGTKTMQPGTTSICNDIIVSGTAQLVAANTLSIGR
                     NVSVGASAKLKGGSHTFNVAGTFQVQGTFEYDSSTVNFNGGSPVVVHPGNFYNIIFSG
                     YGTKTAIGSLNVKGDMTINTKFNAGSFSHSLAGNFVNNGSFIAASSTIVLEGTAMQTI
                     SGSSSTTFHHLTVNGTQDFILATSSVVSGTLNLTKGLLSLGNNNLRVSAVSGGSALSY
                     VKTNGLGTLRRRISGGGSEVVFPVGNSTYNPIGITNTTPADSDYYFVRVVDEIPSLAN
                     DSTKTVYHKWYITEFTPGGSALTIRVTYDLSSGVGANFNPAFTPVIGYYTGSKWAAAM
                     PATVSSYTFSSAVGAFVTDDMTGSDDYFCVGSGDAFSASRLAIVDMRPATPFANHPNV
                     SIMIEAQNSSGIPVDLSSTTTYDLTAFNTSFSAIPSGSISAGASRDTLSNLTFTTAST
                     TATVQADRTSGENLSPLSSDTFVVVSGTLYRPYTNVATYWSNAIWIYSSDGGASWTTV
                     PIQKTDFSASDAVIIPTGYITKVNTTASFYNLRIEPGAELEIVTSGSLTFKHSGSENG
                     LVVQGTLHNSGGTLTNTLKSHPVSVRGGTYIHNKVGDTIPEIEWISRGSSLSTCRIQK
                     IPAHGFNQTFQQLEFAHTGSLSIDGDITVENRLSLINGIVQTGSYKIVLAAQATVSSL
                     NNARIQGNIRAYVPNAIEPALLFPLGDANVYSPMNINFEGSTFGSGYLDASTSVQAPP
                     FASGLSQSKYINRRWTLISSGVEGFTSYSATFGYGNEDKIGNPDYTALVVREFNITTQ
                     TWSVTNTDSLQPNLTRCSGIKHFGCFIIGEDECSAEKSFWLGSINTDWHTGGNWCNGS
                     VPDALQDVYIPAGTANQPIINTTANPATCRDLTIQVGASLTLSSSNLIHVKGDWILQG
                     NFISNQGTVILDGTVPQQMYGASVFCNLTINNPAGVTAHSNLQVNSILDMQSNNPSMS
                     KGVLDMGENVLTMGSGAYTRGPGDVSGSIYRDYFVLDREYSFGHRYTRFYFEDVSGQD
                     LPSTMLVRVTLGNAPDWRSYSGDTMMNAVRRLYEITITGSEKFIPGIKFHYKDNEYAT
                     GLNESNLSIWILKNDSVKELGQAEIDVTNNYLMVENINFGNISNFKLTVAPKKAPLLT
                     WTGNYNKARTDWNMPQNWSPQYTPSSDFRLRIPATTYDPVLPPGASCVSLEIASGGVL
                     NTAANATLVLYGDWVNLAGVSGFNAGNSLITFNANSTVHGSTDFYSLAATDGAQITPA
                     LNAEIGISGTFGLSVTGRLNADEQANTVTFKGTNQYLPTSADCMMEFWNLVISGTGTK
                     YFPAIVEIAGNFTNNGTICTDTCKTKFIFNGTVAQTIGGTTSTHFNHLIINNGSGGSV
                     GLATAATADSLTMTSGVLTTDATNLLSITNTLAGSVSGGSATSYVNGPLKRSLPENLS
                     TAAGYHFPLGKGGLYLPFSLVNPTTGAGAVTAQVEAFAANCGGTADKTTLDSISQSEY
                     WNLSTVGNFINSGVSLHRSSALNALNAIGGCTTLAGVYSNLNGTVQGSSILNSDQIGS
                     YRYFVLAKKFTQITWNGSSSSTWFTATNWTPPVVPGVSSQIIIPNVTTLPVISGSSPA
                     NDLSLAGSLEIKDNATLSLQAGPLLSLQPGITVNTNNSGKIVLESGARYLNLSTGTPR
                     LEIRRELSGGKGWRMLSSPVPATFAQLLPSPLVTQGYTGSTFPTLQPNLLWWDETDAG
                     TTLQSWRQPANSGDSIVKGRGYFYFIFNGAGRLDSAGNSTGQNYTDVLPKTLSVTGVE
                     AFGGSGVFSYNLHFTPRD"
     gene            complement(6521..6943)
                     /locus_tag="PLI37_03265"
     CDS             complement(6521..6943)
                     /locus_tag="PLI37_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOQ58069.1"
                     /translation="MAKTNKVNAIWLRNMAFEAELDGHRLVMDAAAEFGGRDLGPRPK
                     PFMLLALAGCTGMDVVSLLKKMRVEFEAFSVEVEGELSEERPIRYTKMHVIYSFKGRD
                     LPLEKLEKAVKMSEEQLCGVGAVYRKCMEVTSEIRVSN"
     gene            complement(7060..8832)
                     /locus_tag="PLI37_03270"
     CDS             complement(7060..8832)
                     /locus_tag="PLI37_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOQ58070.1"
                     /translation="MSSNFHPFVLPFTIGTLALFSVLILKYNKWISKFDRQQKKLIRR
                     NLFTGKLIPAVWEMINEGLFHKKVSRYNRRLGYMHRSIALGWFLLISVGFVETVFHFQ
                     GRGHAPWIGVFFRFFVRDNQTLSAWIFAQIMDLLLLYILSGVTMAVFKSIFSKTVGMR
                     KTTRLKLFDTSLRYSLWSIFPLRLLSESATAALADNGGFLTQSLGNLMGERFAGVVEL
                     PLWTLYSIALGLFLCLLPFSRFSHIFTELMLIYFRKMGLREREKPTGFTRFELSACSR
                     CGICIDNCPLDSELNITDIQPVYLIRDIRNRMPHRNATENCLMCNRCESDCPVGIQIS
                     EIRRQERDKKKLDTHDNYGYIEDVQSFNAIGRVIYFGGCMSHLTPGITESMKKIFDTV
                     GQKYWMMDEERTICCGRPLLQQGFFNQAAELRRKNTQLIMESRSKTLVTSCPICYQSF
                     RKEYNLPIKVVHHTEYIVTMLRLGKLRLNRSELRLAYHDPCELGRGCGIYDEPREVLS
                     ALGQLLKLEQEYKNSLCCGYNLGNTVLELDQQLKVRDASLRNLTDSHPDVIATACPLC
                     KKAFQHSTTQPIKDVAELVVENLR"
     gene            complement(8842..9174)
                     /locus_tag="PLI37_03275"
     CDS             complement(8842..9174)
                     /locus_tag="PLI37_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOQ58071.1"
                     /translation="MADFGFSISKPQSIDTNDMDVGRLQALEAKVPSFKRCINCGACT
                     ATCSAGQFGGFNIRRIHNLYRWDQRKGLEKELQQCMLCGKCTLVCPRGVNLRYLIIRL
                     RKALADNK"
     gene            complement(9177..10316)
                     /locus_tag="PLI37_03280"
     CDS             complement(9177..10316)
                     /locus_tag="PLI37_03280"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ58072.1"
                     /translation="MKEREKPVHGDDNPKPREVKRRLKSTKRISRRKRLEFEEVELPL
                     QVAIIGGGVAGMEAASVLTEHRCPVILFEARPELGVNVKNKYKIFPDFSDAGRLVETM
                     TKAMDHPLITVNKDTEIVEIIREPDAYTLLDNKGVPHTVPAVLLASGYEPFDAKRKEE
                     LGFGIYEGVINSLQLEDMLREHKVLNSLGEDPRRIVFLQCVGSRDEKVGNHYCSKICC
                     ITAVKQAIEVKRQLPDTEVYVFYMDLRLWGQGYEELYREAQEQYSVRFVRGRISEAAS
                     TFDGRVQIKSEDTLLGQPLKMVTDLLVLMIGMEASPGTQKLGKTCGLEGPYGFVRSKS
                     PHLEDNLSDKPGFFLAGTCKRPMHIAEAVNDGRAAALEIIKYLRK"
     gene            complement(10327..11388)
                     /locus_tag="PLI37_03285"
     CDS             complement(10327..11388)
                     /locus_tag="PLI37_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOQ58073.1"
                     /translation="MTTKKHYWNEYQKEIADDKYFYERSCIRQTFFPGSETAFLRILK
                     EELGKDILDVENQHTCTGIGFYSDIVPLETTMTIVARLFSLMTEAGYENYVPSCVTSF
                     GVFSEIVDLWEHQPDMLEKARKILFETTGRTFELPKNIAHPSDIIYKFREELKEKMRY
                     RLVNRFTGRPLKVVEHIGCHYAKIFPDKGVGKAEFPHVLAGMIESWGGEVVDYPERRH
                     CCGFGFRQYVVRANRGYSVANSKKKFESMQPYEPDFIVTNCPGCTMFMDKWQYTIAEM
                     EHKTYDKKGYGIPVLTYEEMAGLLLGYDPWELGLQLHQVQSERLLDKLGIHYDPNEKY
                     KSVSGRILPNPEKPQYLRV"
     gene            complement(11385..12065)
                     /locus_tag="PLI37_03290"
     CDS             complement(11385..12065)
                     /locus_tag="PLI37_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOQ58074.1"
                     /translation="MGNLYDLLTQDVRFEEALNSCMNCGICTAVCPAAEFYNYDPRKI
                     VDTIQRRNEDEIEALLRSETIWYCGQCLSCKTRCPRGNTPAVLIAILRSVSQKMGYFT
                     ESEKGLQQFAISKKIAQTILDTGYCVHPDTMSYEQHPEQGPVWKWYTKYIEEIAPRFG
                     ANYHGEGPGALRNIRKETLEEINKIFEATGGKELMRKVETGAKNKTNIRNDEDLFNLV
                     YNRNTSKE"
     gene            complement(12086..13315)
                     /locus_tag="PLI37_03295"
     CDS             complement(12086..13315)
                     /locus_tag="PLI37_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOQ58075.1"
                     /translation="MKSQLKCVKCGYISPGFAAWFEQDQTCPKCGSKHSEAEYQADYT
                     QLEALYKQGADSFWHYFDFLPLENRDNIISCKEGAIPIESWDFLEDFAREHYGIECRA
                     LVYRNDLNGGTQTFKDVAGSMAASLFREHGVKQYCIASTGNTATAYAKYLSLAGVAFK
                     VFVPAGANEDSVQEILGFGQEVVRSQGDYARAKQEAADYARQNKVLISAGNFDPIRVE
                     AKKTMVFEFMRQLGKMPEVYIQAVAGGTGPIAIDKGVREIEKYYPEVVLPRMLLVQQD
                     LCDPMVQAWEQAVKEGFPENYHKNYPVIDNPQTKVSILSTGNPGMYPVIAPIVRKTGG
                     SFLRISEASLPALARKVYDQQGVLLGPASIVCLAGFYRALEEKRIHQGDTVLINCGEG
                     MQRAAAFHKEVLAVAGK"
     gene            complement(13312..>14178)
                     /locus_tag="PLI37_03300"
     CDS             complement(13312..>14178)
                     /locus_tag="PLI37_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ58076.1"
                     /translation="VYDSESHACIIDGLRMHFGKRFVFQHNDMDSFRAQMESATALAE
                     QQRGGILVITEGVFGMAGDLGKLDEIVAMKKDFKFRLLVDDAHGFGTMGATGAGTGEH
                     LNCQDGIDIYFSTFAKSMAGIGAFVASDRAIVDHLRFNMRSQIFAKSLPMPMVIGAMK
                     RLELLKTKKELRENLWVIVRALQNGLKEKGFDIGRTQSPVTPVFLKGGVNEGSNIVVD
                     LRESYNIFCSIVVYPVVPRDVIMLRIIPTAAHRMEDVERTINVFSEIKKKLETGEYKR
                     EIPNMNLVNKQK"
     assembly_gap    14179..14278
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14279..14729)
                     /locus_tag="PLI37_03305"
     CDS             complement(<14279..14729)
                     /locus_tag="PLI37_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ58077.1"
                     /translation="MKDIFERIQTTPGPLGQYQGAADGYFMFPKLEGEIQPIMRFNGR
                     DVLVWSLNNYLGLANHPEVRKADADASKEWGLAYPMGARMMSGDTVHHKKLEQDLADF
                     VGKEAGYLLNFGYQGMVSIIDTLLSRRDVVVYDSESHACIIDGLRMHF"
     gene            complement(14948..15904)
                     /locus_tag="PLI37_03310"
     CDS             complement(14948..15904)
                     /locus_tag="PLI37_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOQ58078.1"
                     /translation="MTIQILLLLLGFVLLIKGADWLVDGGAALARKYHVSELAIGLTI
                     IAFGTSMPEMVVNVFAAYKGHADIVYGNILGSNIFNLLGILGIAGLISPLIVQASTVW
                     KEIPLSLLAVVILYLLSNSIFTQNELLSRWDGIILLLVFAFFLYYVFRQLKQENTTSV
                     GEQHKEFTMFKIILFIVLGLAFLVVGGRLVVNNAVKIAEFLGISQTIIGLTIVAVGTS
                     LPELATSVVAAVKKNNDIAVGNIIGSNIFNIFFILGVSALIRPIEYNTKFNFEMYFLS
                     FGTILVFIAMFSGKKRRLDRWEAGILLLMYVGYTILLINEQV"
     gene            complement(15930..16754)
                     /locus_tag="PLI37_03315"
     CDS             complement(15930..16754)
                     /locus_tag="PLI37_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOQ58079.1"
                     /translation="MPNSKGLTLEHKEERQRITQVKTPGMYAFWTSNEDNKGSAVLIC
                     PPGGYQKLTYHLAGFQWAKWFNTMGVNAFVLIYRLPNSPDLIEREKGPIQDAQRALKI
                     IRNMASVRNIDPGKVGIVGASAGGHLASTLGTHFEDFSLIGDSIDRYSVRPDFMVLIS
                     AVINMGDFAHEGSVQAFLGADAGKEKRDYYSNEKQVTEQTPPTFLVHARNDNTVPVQN
                     SIQFYNAMLEKGVDGSLHIFPYGRHSISLKNESVMLNYWTSLCENWLYEMGLLKQK"
     gene            complement(16897..18012)
                     /locus_tag="PLI37_03320"
     CDS             complement(16897..18012)
                     /locus_tag="PLI37_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58080.1"
                     /translation="MKHSKLFFLRALLLVLFAGFDLAASAQANRADYTGHTNRTNPPA
                     KLKLLSAHPREVIGPEHPDVILSRNFSGFETGQVIKMDGTYHMFVNEMFVRPFRDLRI
                     SYWTSKDALNWERRATIRESIPGRSSYNPRSEVWVTGVEFNEDENAWNIFYVAYRAGD
                     ETKGEIARSDYSGRIWRAKSVIPGIKGIAGPYADMGIVLQPGEHSQAWEGQQAVASFN
                     PYKVGDTWYAFYDGHNYIPQGGWPTGMARAEKLEGPWTRMPEDFNPLPIVDEFMENTV
                     ITQLKNGKHLAVFDSFGDREIGYSLSEDGLNWSKETRIKVQFEDRAWAKDGNHSLRTP
                     LCAIEEDDGTFTVIYTGLMDRREEAFYAVGKCTLAWE"
     gene            complement(18165..19508)
                     /locus_tag="PLI37_03325"
     CDS             complement(18165..19508)
                     /locus_tag="PLI37_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOQ58081.1"
                     /translation="MSIHASTENAQTKTLLSGEPGDPVRSICFLPLDSIAFSADLGEF
                     KPFVNISSDTLPYATFSGDLIDAGMYVAFYPWKLALNLNRDTLLTLKLPYTQQYKAGG
                     FASGAYPMVSWSYDTNLHFKNLCGLLKLNLCGDVSVSSLSLCAPGLAGVAGVDMRYQG
                     VPQLIMPTPPAYDTLTLDCGEGVQLDDEQATAFYFVLPPALYESVTLIVRTTDHQQMT
                     ETTQNLRIVRSSITPTSVIEFDSDDNPNPPGNTVLDIQGNVYKTVAIGNQIWMAENLK
                     STLLNDGTEIPHITDNSSWNTLTTPGYCRYNNDPALYSDYGLLYNWFTVETGKLCPQG
                     WHVPSHAEWTELTDYLGGESIAGDKLKESGTGHWAGSNPGSTNETGFTGLPGGLRNKY
                     GGFQDIGYGGFWWSATEGDGGGVWYRYVYFDYSAVYAYDNGLKEFGFSVRCVKSD"
BASE COUNT         5025 a         4779 c         4671 g         5116 t
ORIGIN      
        1 ctgtcgcggg gcgtaaaatg gaggttgtag ctgaaaacgc cggaaccgcc gaaggcttcg
       61 acacctgtca cagagagagt ctttggcagt acatcggtat aattctggcc ggtagagttt
      121 ccggctgaat cgagccgccc ggcgccatta aaaataaagt aaaaatagcc tctgcccttc
      181 acaatactgt cgccactgtt ggcgggttgt ctccagctct ggagagtggt tcccgcgtcg
      241 gtttcgtccc accacaacag attgggttgc agcgtgggaa aggtcgaccc ggtatagccc
      301 tgggtgacca agggcgaggg caacaattga gcgaaagtgg ccggtaccgg cgaggaaagc
      361 atgcgccagc cctttccgcc gctgagttcg cgccgtattt cgagccgggg agtaccggtg
      421 cttaaattca ggtaacgggc acccgattcg agaacgattt ttccgctgtt gttggtatta
      481 accgtgatac ccggctgcaa gctgagcagc gggcctgcct gcaaacttaa ggtggcgtta
      541 tctttgatct ccaggctccc tgccaggctc aggtcgttgg ccggactgct gcccgaaata
      601 acgggaagag tcgtcacgtt gggtatgatt atctgactgg aaacgcccgg aactaccggc
      661 ggggtccagt tggttgccgt aaaccaggta ctggaagagg agccgttcca ggtgatttgc
      721 gtaaattttt tagccagtac aaaataacga tagctgccga tctgatcgga attcagaata
      781 gaactgccct gaaccgtacc gttgagatta ctgtacacgc ctgccagggt agtacagccc
      841 ccgatggcgt taagtgcatt cagcgccgaa gagcgatgca aggaaacgcc gctattgata
      901 aaattgccca cagtacttag gttccaatac tcgctttgac tgatcgaatc caatgtggtt
      961 ttgtcggctg ttcctccgca gtttgcagcg aaagcttcca cctgggcagt aaccgcacct
     1021 gcgccggtag taggattgac caaagaaaaa ggcaggtaga gccctccttt acccaagggg
     1081 aaatggtagc cggccgcagt gcttaggttt tcgggcaaac tgcgtttaag agggccgttc
     1141 acataagaag tagcggaacc gcccgaaact gaaccggcca gggtattcgt gatgctgaga
     1201 agattagtag catcggtggt caaaaccccc gaggtcatgg tcaggctgtc ggctgtggca
     1261 gcagtagcca gaccgacact gcctccgctg ccgttgttta ttatcagatg attgaagtgt
     1321 gtagaagtgg tgccccctat ggtttgagct acggtaccgt tgaatatgaa tttggtttta
     1381 caggtatcgg tgcaaatagt accgttgttg gtaaaattac cggctatttc cactatggcc
     1441 ggaaaatatt ttgtacctgt tccgcttatg accaggttcc aaaactccat catacagtcg
     1501 gccgaagtgg gtaaatattg atttgtacct ttaaacgtta cggtgtttgc ctgctcatcg
     1561 gcattcaatc ttccggtaac ggaaagaccg aacgttccgc tgattccgat ctccgcattc
     1621 agagccggag tgatttgtgc cccgtcggtt gccgccagac tgtaaaaatc ggtgctgccg
     1681 tgaacagttg agttggcatt gaaagtgata aggctgttgc cggcattaaa cccggaaact
     1741 ccggccaggt ttacccaatc gccgtaaagg acgagcgtgg cattggctgc tgtattcaaa
     1801 accccgccgg aagctatctc gagcgaaaca cacgaagctc ccgggggtaa aaccggatca
     1861 taggttgttg caggtattct taacctgaaa tcgctcgaag gagtatattg aggactccag
     1921 ttttgcggca tattccagtc ggtccgagcc ttattgtagt tacccgtcca cgtcagtaaa
     1981 ggagcctttt ttggggcaac ggtaagtttg aagttgctga tgtttccgaa attaatattc
     2041 tctaccatca ggtaattgtt ggttacatcg atctctgcct gtcccagttc ttttaccgaa
     2101 tcatttttca aaatccagat actcaaattg gattcgttca ggcccgtagc gtattcgttg
     2161 tctttgtaat gaaacttgat gccgggaata aacttttcgc tgccggtaat agtgatttcg
     2221 tagagcctcc tgactgcatt catcatggtg tccccgctgt aggatctcca gtcgggcgca
     2281 ttacctagag ttacccttac cagcatggtg ctgggaagat cttgtccact gacatcttcg
     2341 aaataaaaac gggtatatct atgtccgaaa ctgtattcgc gatctaaaac aaaataatcg
     2401 cgataaatgg agccgcttac atcgcccggg ccgcgagtat aggctcccga gcccatagtc
     2461 agcacatttt cacccatatc gaggacgccc ttgctcatag aagggttatt gctttgcata
     2521 tccaaaatgg agttgacttg cagattgctg tgagccgtaa caccggcagg attgttgatg
     2581 gttaaattac aaaagacact cgcaccatac atttgttgcg ggacggtgcc gtccagaatc
     2641 acagtaccct gattggatat gaaattacct tgcagtatcc aatcgccttt gacatggatc
     2701 agattggagg aactcaaagt caaactggct ccgacctgga tggtgaggtc cctgcatgtg
     2761 gctgggtttg cggtagtgtt gataatgggc tgattggctg ttccggcagg gatatagaca
     2821 tcttgcaaag catcgggaac agagccgttg caccagtttc ctcctgtgtg ccagtcggta
     2881 ttgatacttc ccagccaaaa agatttttca gcggagcatt cgtcctctcc tatgataaag
     2941 caaccgaagt gttttatacc actgcagcgg gtcaaattgg gttgcaaact gtcggtattg
     3001 gtcaccgacc aagtttgggt ggtaatatta aactcgcgta caaccagggc ggtataatcg
     3061 ggattgccga ttttgtcttc gttcccgtaa ccgaaagtag cactgtaaga agtaaaaccc
     3121 tccactcccg atgaaatgag tgtccatcta cgattgatgt atttgctttg acttaaaccc
     3181 gaagcaaaag gtggtgcctg gaccgaggtg gaagcatcca gataacccga accgaaagtg
     3241 gagccttcga agtttatgtt catcggactg taaacattag cgtctccaag cgggaaaagc
     3301 aatgcaggct ctatagcgtt gggaacataa gccctaatat taccttgaat acgggcgttg
     3361 tttaaactgg agactgttgc ctgcgctgcc agtacaattt tgtaactgcc ggtttgcact
     3421 atgccgttga tcaggctcag ccgattttcc actgttatat cgccgtctat gctcaggctt
     3481 cctgtatgag caaattccaa ctgttgaaaa gtctgattga atccatgagc gggtattttt
     3541 tggatgcggc aggtggataa gcttgatccc cggctgatcc attcgatttc aggaatggta
     3601 tcgcccactt tgttgtggat ataagtgcct ccccgtaccg aaacagggtg tgatttgagg
     3661 gtattggtaa gagttccgcc cgaattgtgc agagtgccct gtaccacaag gccgttttcg
     3721 ctgccggaat gtttaaaagt cagggatccg gaagtaacaa tttccaattc ggcccccggt
     3781 tcaattctaa gattgtaaaa agaggccgtg gtgttgactt tggttatata tccggtagga
     3841 atgataacgg catcgctggc cgaaaaatcg gttttttgaa tcggaacagt ggtccagctt
     3901 gctcctccgt cactactgta aatccaaatg gcattggacc aataggtcgc cacgttggta
     3961 taaggcctat acaaagtgcc gctgaccacc acgaaagtgt cgctacttaa cggagacaaa
     4021 ttttcgccac ttgtgcggtc ggcctgcaca gtggctgtgg tactggccgt ggtaaaagtc
     4081 aggttgctca gcgtgtcgcg cgatgcaccg gcagaaatac tgcccgacgg aatagccgaa
     4141 aacgaagtat tgaaggccgt tagatcgtag gtcgtagttg agctgagatc tacaggaatg
     4201 cccgagctgt tttgagcttc gatcattatg gacacattgg gatgattggc aaagggagtg
     4261 gccggcctca tatctacaat ggccagtctc gaagcgctaa aagcatctcc ggaacccacg
     4321 caaaaatagt cgtcgcttcc ggtcatatcg tccgtaacaa aagctccaac agcagaacta
     4381 aaggtataac tgctaacagt agcgggcata gctgccgccc atttactacc ggtataatac
     4441 ccgattaccg gcgtaaaggc cggattgaaa tttgcaccga ctcccgacga aagatcataa
     4501 gtaacacgaa ttgtcaaagc agagcctccg ggtgtaaatt ccgtaatata ccatttgtgg
     4561 tatacggttt ttgtcgaatc gttggccaac gaaggtattt cgtccacgac tcgcacgaaa
     4621 taataatcac tatcggccgg agtcgtattg gtaataccaa ttggattgta agtggaattt
     4681 cccacgggaa acaccacttc gctcccgccg ccgctaattc ttcgtctcaa tgtgcccagg
     4741 ccattggttt tgacatagct caatgcgctt ccgcccgaaa cggccgaaac cctgagattg
     4801 ttgtttccca aactcagcaa acccttggtc aggttgagcg ttccgcttac caccgacgaa
     4861 gtggccagta taaaatcctg ggtgccgttg acagtaagat ggtggaatgt tgtggaagag
     4921 cttccgctaa tggtttgcat cgcggtgcct tccagtacaa tggtgctgct tgccgcgatg
     4981 aaactgccgt tgttaacaaa attgccggcc agtgagtggc tgaagctgcc cgcattaaac
     5041 ttggtattaa tggtcatatc gcccttaacg ttcaggcttc ctatggctgt tttggttccg
     5101 taaccactaa aaattatgtt gtaaaaatta ccggggtgaa ccacgacagg actaccccca
     5161 ttgaaattga ccgtagaaga atcatactcg aaagtgccct gcacctgaaa agtgcccgcc
     5221 acgttgaaag tatggctgcc gccctttagt ttggccgagg caccgacgga gacattacgc
     5281 ccaatgctca gtgtgttggc tgcaacaagt tgggctgttc ccgacacgat aatatcgttg
     5341 cagatggaag tggttccggg ctgcatggtt ttggtaccac tacccgacaa cagcaaattt
     5401 ccgtagttta ttcctctgac tatctggttg ctgccgtagt attcaatgtt gctggtacaa
     5461 tcgaggatgg gaacagaata attggcagaa atcgaaccgg ttccgccaat tcgtaaggta
     5521 gttccggctt cgatagacaa ggtaccccca ctggtagcgc gactcagtgt gtaggtttgc
     5581 atatcaaaag tacccgatcg aataaagatg tttgtgagag ctgttttatt gcccgacaga
     5641 ctgacggtat acccggtgcc cggatcaata atcaatccgc cggctcctcc aaaattggcg
     5701 ggaaattccg ttccggtagt tctgttgccg gtatttttgt attccaacga agaagccggt
     5761 ccaaaactta gggtatagcc tccgttggcg aaatacgccg tttgccttaa cgataagtaa
     5821 ccgttgacgg taaggttgga agcaagcgtt ttggtaccgc tgccctccaa caccagatcg
     5881 tttactctcg tgggcaaacc gcttaaggtt tggttggtgc taccgttaaa ataataatta
     5941 gccgcattgt ccagcgctat aatgatgttg gttgtgcgta gggattgtgt gcctgttccc
     6001 agcagtccgg ctgtattcgg tgttttgacg gtagcacccg gcagaagcga aaaccttgtt
     6061 tctccattgt tggcaccttg ataggttccg atcagttgat tgataccgct gtctttaaat
     6121 atcagactgc cgcgaactat catggtggta tagcctttgt tctttctgta aagcgtcata
     6181 acaaccccct gattgatact gagcgaccca ttgacctcta aggtcatcac tgaattctga
     6241 atgtgattga aaatccagtt tgcgcttaca gtaccgtcaa tatccggagg aataatgaat
     6301 atatccccgg aaacagtgaa gctgccggcc gccgtaccgc taccgtcggg ttcggtattc
     6361 cagttggatg ctacctgcgc actgccggca ccgctggctg tcaaataata ggtcgcggac
     6421 tcaactctgc ccggaaagga cagcagtaac aacgcgacaa ttattatttt caagaccggg
     6481 gacttcatat tgctcaaact ccttcctgtc tataaattcc tcaattcgat acacgaatct
     6541 cagaagtaac ttccatacat ttccggtaaa ctgctcctac cccacacaac tgttcttcgg
     6601 acattttcac ggccttttcc agcttttcca ggggcaggtc gcgtcccttg aaactataga
     6661 taacgtgcat tttggtatag cggataggtc tctcctcgct gagttcaccc tctacctcga
     6721 cactaaaagc ttcgaactct acacgcatct ttttgagtaa acttaccaca tccatacccg
     6781 tgcagccggc cagtgccagc agcataaagg gtttgggcct ggggcctaaa tcccggccgc
     6841 caaactcggc agcagcgtcc attacgagac ggtgcccgtc gagctctgcc tcaaaagcca
     6901 tattccggag ccagattgca ttgaccttat ttgttttggc catatacttt tggttttttt
     6961 tatcaattcc ttatttcata agccggaacg aacatatgtc cgataacacc acgacaaata
     7021 tagtcttttt ttaaacagga ggcgatcgga acacgtattc taccgaagat tttccaccac
     7081 cagctcggcc acgtccttga ttggctgtgt agtggaatgt tgaaaagctt ttttgcacaa
     7141 agggcaggcc gtagcaataa cgtcgggatg agagtccgta agattacgga gcgaagcatc
     7201 ccttaccttt aattgttggt cgagttccaa cacggtattg cccaggttat aaccgcaaca
     7261 gagactgttt ttgtattctt gttccagctt caacagttgc cccaaggccg aaagcacttc
     7321 gcggggttcg tcgtaaatac cgcagcccct gcccaactcg cagggatcgt ggtaggccag
     7381 cctcagttcg ctgcggttga gcctgagttt acccaggcgc agcatggtta caatgtattc
     7441 ggtgtggtgt actaccttga tgggcagatt atattctttg cggaaagatt ggtagcaaat
     7501 ggggcaggaa gtcaccaggg ttttggagcg cgactccata atgagctgag tatttttgcg
     7561 cctcagttcg gctgcctgat tgaaaaagcc ctgctggagc agaggacgtc cacagcatat
     7621 ggtcctttct tcgtccatca tccagtattt ctgtcctacg gtgtcgaaaa tctttttcat
     7681 cgattcggta atacccggcg tgagatgcga catgcagccc ccgaaataaa tgacccggcc
     7741 aatggcattg aaggactgta catcttctat ataaccgtaa ttatcgtgag tgtctagctt
     7801 ttttttgtct ctttcctggc ggcgaatttc ggaaatctgt atgcctaccg gacaatcgct
     7861 ttcgcaacgg ttgcacatca ggcaattttc ggtggcgttg cgatgaggca tgcgattgcg
     7921 tatatcgcga attaaataaa ccggctgaat atcggtgatg ttgagctcgg agtcgagggg
     7981 acaattgtcg atgcaaatac cgcagcgcga acaggccgaa agttcaaatc gagtaaaacc
     8041 ggtgggcttt tcccgttcac gtaaacccat tttgcggaaa taaatcagca tcagttcggt
     8101 aaaaatatgg gaaaaacgag aaaaaggcag gagacagaga aacaaaccca gggctatgga
     8161 atacaaggtc cataaaggca attccaccac cccggcaaaa cgttctccca ttaaattacc
     8221 caagctttga gttaaaaaac ctccgttatc ggccaaagcc gcagtggcac tttcggaaag
     8281 caggcgcaaa ggaaaaatag accaaagcga ataacgcaaa ctggtatcaa acaacttcag
     8341 acgcgtagtt ttgcgcatgc ctacagtttt tgaaaaaatc gatttaaaca ccgccattgt
     8401 cactcccgac aatatataga gcagcaacaa atccatgatc tgagcaaaaa tccaggccga
     8461 aagagtttga ttatcgcgta cgaagaaacg aaagaaaacg ccaatccagg gagcatgtcc
     8521 ccggccctga aaatgaaata cggtttcgac aaaaccaacg cttataagca aaaaccagcc
     8581 caaggcaatg ctacggtgca tataacccaa acggcggttg tatctgctta cttttttgtg
     8641 aaacaaacct tcgttgatca tttcccaaac tgccggaata agcttaccgg taaataggtt
     8701 gcggcgtatg agtttttttt gctgacggtc gaacttactg atccacttat tgtacttaag
     8761 aatcaaaacg ctgaacaagg ccagcgtacc gatggtgaaa ggaagtacaa aaggatgaaa
     8821 gttggaagac atgggttcag cttatttgtt gtcggccagg gctttgcgta aacgaataat
     8881 aaggtaacgc agattcactc cgcgggggca gactagggta cacttgccgc atagcatgca
     8941 ttgctgcaac tctttttcca atcctttgcg ctgatcccaa cgataaagat tgtggatgcg
     9001 acgtatgttg aagccaccaa actgaccggc agaacaagtt gccgtgcagg ctccgcagtt
     9061 gatgcagcgt ttgaaggacg gaactttggc ttccaaagct tgcaagcgac ccacatccat
     9121 atcgttggtg tcgatcgact gaggttttga aatactgaat ccaaagtcgg ccatagttat
     9181 tttctcaagt acttaataat ttccaaagcc gctgcacggc cgtcgttcac ggcctcggcg
     9241 atatgcatgg gacgtttgca ggtgccggcc agaaaaaaac ctggcttatc gcttaggtta
     9301 tcttccagat gcggactctt gcttcgaaca aaaccgtagg gcccttcgag tccgcaggtt
     9361 ttgcccagtt tttgggtacc cggggaagct tccatgccta tcatcaacac cagtaagtcg
     9421 gtaaccatct tgagcggctg ccccagtaaa gtatcttcgg attttatctg tacgcgaccg
     9481 tcgaaggtgg aggcggcttc cgaaatgcga ccccggacaa aacgcacact gtattgttcc
     9541 tgcgcctcgc gatacaactc ctcgtagccc tgaccccaaa gtctcaggtc catataaaag
     9601 acatagactt ccgtatcggg aagctgacgt tttacttcta tagcctgctt tactgcagta
     9661 atgcaacata tcttggaaca atagtgattg cccacttttt cgtcgcgcga acctacgcat
     9721 tgtaagaaga ctatgcgcct ggggtcttcg cccaggctat tcaagacttt gtgttctctg
     9781 agcatatctt cgagctgcaa tgaattgata acgccttcgt aaataccaaa acccagttcc
     9841 tccttgcgtt tggcatcgaa aggctcgtaa ccgctggcca gcaaaacagc cggtaccgta
     9901 tggggcacac ccttgttgtc gagcaaagta taagcatcgg gttcccggat gatctctacg
     9961 atctcggtgt ctttgtttac agttatcagg ggatgatcca tcgctttggt catggtctct
    10021 accaaccggc cggcatccga aaaatcggga aaaatcttgt atttgttttt cacgttgacg
    10081 cccagttccg gacgggcctc aaacagaatg accggacaac gatgttctgt cagcaccgaa
    10141 gcggcttcca tgcccgccac tccccctccg atgatggcca cctgaagggg taattccacc
    10201 tcttcaaact cgaggcgttt tctgcgactt atccttttgg tggatttcag cctgcgtttt
    10261 acttctctgg gtttggggtt gtcgtctccg tggaccggtt tttctctttc tttcatctcc
    10321 aaagtattaa acacgtagat actgaggctt ttcgggatta ggtaaaatcc tgccgcttac
    10381 acttttgtac ttttcattgg gatcgtaatg aatgcccagc ttgtcgagca ggcgttccga
    10441 ctgcacctga tgcaattgca gtcccaattc ccaggggtcg tagcccagta aaagcccggc
    10501 catttcttcg taagtgagta ccggaatgcc ataacctttt ttatcgtagg ttttatgctc
    10561 catttcggct atggtgtatt gccatttatc cataaacatg gtgcaacccg gacagttggt
    10621 cacgataaaa tcgggctcgt agggttgcat cgactcaaat ttctttttgg aattagccac
    10681 cgaataaccc ctgttggctc tgaccacgta ttgcctaaaa ccaaaaccgc aacagtgacg
    10741 gcgctcggga taatctacca cctcgcctcc ccaggattcg atcatacccg ccagcacatg
    10801 cggaaattcg gctttgccca cccctttgtc ggggaaaatt ttagcgtaat ggcagcctat
    10861 gtgttccacc accttcagag gccggccggt gaaacggttg accaggcgat agcgcatttt
    10921 ctctttgagt tcttcgcgaa acttatagat aatatccgaa ggatgagcga tgtttttggg
    10981 caactcaaaa gttctgccgg tggtttcgaa taagatcttc cgggcttttt cgagcatatc
    11041 cggttgatgt tcccagagat cgacgatttc ggaaaaaacg ccgaaagaag taacacaaga
    11101 gggcacgtag ttttcgtaac cggcttcggt cataagcgaa aacagacggg ccacgatggt
    11161 catggtggtt tccagtggca caatatcgga atagaaacct atgcccgtac aggtgtgctg
    11221 gttttccaca tccagaatgt ccttgcccag ctcttctttg agtattctca aaaaggccgt
    11281 ttccgatccc gggaagaagg tctgccggat gcaactgcgc tcgtaaaaat acttatcgtc
    11341 ggcaatctct ttttggtatt cgttccaata atgttttttt gtagtcattc tttggaagta
    11401 tttctgttat agaccaaatt aaacaaatct tcgtcgtttc tgatgttggt tttgtttttt
    11461 gccccggttt ccactttgcg catcagctct ttgcctccgg tggcttcaaa aatcttgttg
    11521 atctcttcca gggtttcctt gcggatgttg cgcaaagcgc ccgggccttc accgtgataa
    11581 ttggcgccaa agcgtggggc tatctcctca atgtactttg tataccactt ccataccggc
    11641 ccctgttcgg gatgctgttc gtagctcatg gtatcgggat gtacacaata gcctgtatcc
    11701 aaaatcgttt gggctatttt tttggagatg gcaaattgtt gcaggccttt ttcgctttcg
    11761 gtgaaatagc ccatcttttg ggaaacactc ctcaggatag ctatcaatac tgcgggagta
    11821 ttgcctcgcg gacaacgggt tttacacgag agacactgcc cacaatacca aatggtttcg
    11881 gaacgtaaca gggcttctat ttcgtcctcg ttacgacgtt gaatggtatc gacaatcttg
    11941 cgcggatcgt aattgtaaaa ctcagccgcc gggcaaacgg cggtgcaaat tccgcaattc
    12001 atacaggagt tcaaggcttc ttcgaagcgg acatcctgag tgagtaaatc gtataaattc
    12061 cccattagct atgttttaat ccgatttatt taccggcaac agctaacact tctttgtgaa
    12121 aagcagctgc acgttgcatg ccttcgccgc agttaatcag tacggtatcg ccctgatgga
    12181 tacgtttttc ttccaatgct cggtaaaaac ccgccagaca tacaatggag gccggcccga
    12241 gtaacacacc ctgttgatcg tagaccttac gtgccagagc aggcaagctc gcttcgctga
    12301 tacgcaaaaa actgccgccg gttttccgta ctataggagc aataaccgga tacataccgg
    12361 gattgcctgt tgacaagatg gacactttgg tttgaggatt gtctataacc gggtaattct
    12421 tgtggtaatt ttcggggaag ccctctttga cggcctgctc ccaggcctgc accatgggat
    12481 cgcaaaggtc ttgctgcacc agtaacatcc ggggcaaaac aacttcggga taatactttt
    12541 ctatttcgcg cacccctttg tcgatcgcaa taggacctgt gcccccggct actgcctgaa
    12601 tatacacctc gggcatttta cccaattgac gcataaactc aaaaaccatg gtttttttgg
    12661 cttccacccg tatgggatcg aaattaccgg cagaaatcaa cactttgttt tgacgggcgt
    12721 agtcggccgc ttcctgtttg gcccgggcat aatcgccttg cgagcgaacc acctcctggc
    12781 cgaaacccag aatttcctgc acagagtctt cgttggcgcc ggcaggcaca aatactttga
    12841 aagctacacc cgccaaagag aggtatttgg cgtaagcggt agcggtgttg ccggtggagg
    12901 ctatgcaata ctgctttact ccatgttccc tgaataagga ggcggccatg gaaccggcta
    12961 cgtccttgaa ggtctgtgtg cctccattca aatcgttgcg atataccaat gcccggcatt
    13021 cgataccgta atgctcgcgg gcaaaatctt cgagaaaatc ccaagattca atgggaatgg
    13081 ctccttcttt gcaggagatt atgttgtcgc gattttcgag cgggagaaaa tcgaaataat
    13141 gccagaagct gtcggctccc tgcttgtata aagcttccag ttgcgtgtaa tctgcctggt
    13201 attctgcctc ggaatgtttg ctaccgcact tcggacaggt ctgatcctgt tcaaaccagg
    13261 cagcaaagcc ggggcttata tagccgcatt taacacactt gagctgagat ttcatttttg
    13321 cttatttacc aggttcatat tgggaatttc ccgcttatac tcgccggttt ccagtttttt
    13381 ctttatctcg ctgaacacgt tgatggtacg ctctacgtcc tccatgcgat gtgctgccgt
    13441 cggaatgatg cgtagcataa taacatctct gggtaccacg ggatagacca ctatcgaaca
    13501 gaatatattg tagctttcac gtaaatctac tacgatattg gaaccctcgt tgacaccgcc
    13561 cttcaggaag acaggtgtta ccggcgattg ggtgcgcccg atatcaaaac ctttttcctt
    13621 gagcccgttt tgcagagcac gaactataac ccaaagattt tcgcgcaatt cctttttggt
    13681 tttgagcaat tccagcctct tcatagcgcc gattaccatg ggcatgggca atgatttggc
    13741 aaaaatctgg gaacgcatgt tgaaacgaag atgatcgaca atagcccggt cggaagccac
    13801 aaaagcgcca ataccggcca tacttttggc aaaagtggag aaataaatgt cgataccgtc
    13861 ctggcaattc aaatgttcgc cggtgccggc ccccgttgcg cccatggtac cgaaaccgtg
    13921 agcatcatct accaataaac ggaacttgaa atcctttttc atcgccacaa tctcgtcgag
    13981 tttgcccagg tcgccggcca taccaaatac gccctcggtg atgaccagaa ttcctcccct
    14041 ttgctgttcg gcaagggctg tagccgactc catttgggcg cggaagctat ccatgtcgtt
    14101 gtgctggaac acaaaacgtt taccaaaatg catcctcaat ccgtcgatga tacaggcatg
    14161 cgattccgag tcgtaaacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca
    14281 aaatgcatcc tcaatccgtc gatgatacag gcatgcgatt ccgagtcgta aacaacgacg
    14341 tcgcgacggc tcagcaaggt gtcgataatg gacaccatac cctggtaacc gaaattaagc
    14401 aaatatccgg cttccttgcc tacgaaatcg gccaggtctt gttccagttt cttgtggtgc
    14461 acagtgtcgc ccgacatcat acgggcaccc atgggataag ccagacccca ttcctttgag
    14521 gcatcggcat cggccttacg tacctcggga tggttggcca aacccaggta attgtttaag
    14581 ctccacacca gcacgtctct accattgaaa cgcattatgg gttggatttc accttcgagt
    14641 ttcgggaaca taaaatagcc gtcggcagcg ccctgatatt ggcccagcgg acccggagtt
    14701 gtttgaattc tttcaaaaat gtccttcatt actagtcttt taaaagattg tgtgatataa
    14761 aaaaataaaa attgttttag ttttgaatta aatttcctct ctttatcaat aagttatcag
    14821 tcctatgcaa aaaaaggcat attattcgca agggtatact taacaaccga caaaggtaac
    14881 actacgagcc atactcttct aattcttaac aatatttaat tggctgcgag gaccgggacc
    14941 gggcttttca tacctgttcg tttatcaaca aaatcgtgta cccgacgtac atcaaaagca
    15001 gaataccggc ttcccagcgg tcgagccgtc ttttcttgcc cgaaaacatg gcaataaaaa
    15061 ccagaatggt tccgaagctc aggaaataca tttcgaaatt aaacttggta ttgtactcaa
    15121 taggcctgat caaagcactg actcccagaa taaagaaaat attgaaaata ttggaaccga
    15181 tgatattccc cactgctata tcgttgtttt tcttaacggc agccactacc gaggtggcca
    15241 gctcaggcag cgaagtccca accgctacta tggtcaatcc tatgatagtc tggctaatgc
    15301 caagaaactc ggcaattttc accgcattgt ttacaaccag cctgcctcct actaccaaga
    15361 acgccaatcc cagaacaata aacaatatta ttttgaacat ggtgaattct ttgtgttgtt
    15421 cccccacaga agttgtgttt tcttgtttca attgcctgaa tacatagtag agaaaaaagg
    15481 caaacaccag caaaagaata atgccatccc acctggacaa caattcgttt tgggtaaaaa
    15541 tactattgga aagaagatag agtataacaa ccgccagtaa ggataaaggt atttctttcc
    15601 aaaccgtact ggcttgaact atcaggggag aaatcaatcc ggcaatacca agtatgccca
    15661 aaagattgaa aatattactc cccagaatat ttccgtaaac gatgtcggca tgccctttgt
    15721 aagcagcaaa cacgttgacc accatttcgg gcatagaagt accgaaagca atgatagtaa
    15781 ggccaatagc caattcagaa acatggtatt tgcgagccag tgccgcacct ccgtcaacca
    15841 accagtcggc tcctttgatc aacaaaacga atccgagcag caacaacaaa atttgtattg
    15901 tcataattgt caaaataaaa acacgttcat tatttctgtt tcaacaggcc catttcgtag
    15961 agccagtttt cacataagga tgtccaataa ttgagcatga ccgattcgtt tttcaacgaa
    16021 atggaatgcc ggccataggg gaaaatatgt aaactaccgt caacaccctt ttcgagcatg
    16081 gcgttgtaga attgaatact gttttgtaca ggcacggtat tatcgttgcg ggcgtgaaca
    16141 agaaaggtgg gcggcgtttg ttccgttacc tgtttttcgt tggaataata atcgcgcttt
    16201 tccttgccgg catcggctcc cagaaaagcc tgcacactcc cttcgtgggc aaaatcgccc
    16261 atattgataa cggccgagat caacaccata aaatcgggcc gaacggaata gcgatcgatg
    16321 gaatctccga taagcgaaaa gtcttcgaaa tgcgttccga gggtcgatgc cagatgccca
    16381 cccgccgagg ctccgactat tcccactttg cctgggtcga tattccggac agaggccata
    16441 tttcgaatga ttttaagcgc tctttgggca tcctggatag gccctttttc tctttcgatc
    16501 aggtcgggag aattcggcag gcgataaatc agcacaaaag catttacacc catggtattg
    16561 aaccatttag cccattgaaa accggccaga tggtaggtca gtttttgata gcctcccggg
    16621 ggacaaatca aaacggcact gcctttatta tcttcgtttg atgtccagaa agcatacatg
    16681 cccggagttt tcacctgtgt aattcgctgc ctctcctcct tgtgttccag ggtcaaccct
    16741 ttggaattcg gcattttgcc ttccggccaa agcggtatga attcctgggc aggggcatta
    16801 aaaaaaagac ataagcccgg caaaaagaat agcaaaaatt tagtccgtgg tcggttcatt
    16861 ttttctgcaa tatgatctgt tgattattct cattgattat tcccaggcca aagtgcattt
    16921 tcccaccgca taaaaggctt cctcccgccg gtccatcaat ccggtataaa ttacggtaaa
    16981 agtgccgtcg tcctcttcga tggcacagag cggggtgcgt aaagaatgat ttccgtcttt
    17041 ggcccaggcc cggtcttcga actgcacttt gattctggtt tctttgctcc agttcagacc
    17101 atcttcggat agactgtagc cgatttcacg atcaccaaaa gaatcgaaca cagccaggtg
    17161 ttttccgttt ttcaactgag taataacggt attttccata aactcgtcca cgatgggcaa
    17221 aggattgaaa tcttcgggca tcctcgtcca gggaccttcc agcttttccg cccgtgccat
    17281 tcccgtcggc caacctccct gaggaatgta attgtgcccg tcgtaaaagg cataccaagt
    17341 atctcctact ttgtacggat tgaagctggc aacagcctgt tgtccttccc aggcctgaga
    17401 atgttcgccg ggttgcaaga ctatacccat atcggcgtaa gggcctgcaa tgcccttaat
    17461 tccggggatg acagacttgg cacgccagat tctgccacta tagtccgacc gggctatttc
    17521 tccctttgtt tcgtctccgg ccctataggc aacataaaaa atattccagg cgttttcgtc
    17581 ttcgttgaat tcaactccgg taacccagac ttcggaacgc ggattgtagg aggatctgcc
    17641 gggaatactt tcccttatgg tcgcccggcg ctcccaattc agcgcatcct tgcttgtcca
    17701 gtaagaaata cgcagatcgc gaaaaggacg cacaaacatt tcgtttacaa acatatggta
    17761 agtaccgtcc attttaataa cctgaccggt ctcaaaaccg gaaaaatttc tgctcagaat
    17821 cacatcaggg tgctcaggac caattacttc ccggggatgg gcagacagaa gtttgagttt
    17881 agcgggagga ttggtgcgat tggtatgacc agtgtaatct gcacgatttg cctgagcaga
    17941 ggctgccaaa tcgaaaccgg caaagagaac caacaataat gctctcaaaa agaataactt
    18001 agaatgtttc acttcagaag cgcttgaaaa agacgggcaa aggtacaaaa taatttaagc
    18061 cgtatgctat ttccgtcgga agtaatgttg ccggccctta gcgctgtggt tgcggtgatc
    18121 aatgcaagcg gatcggttac aaagcttgaa atgaatagtg atgattagtc gctcttaacg
    18181 caacgaacag aaaacccaaa ctccttaaga ccattgtcgt aagcgtacac agcactgtaa
    18241 tcgaagtata catatcggta ccagacgcca ccaccatcgc cctctgtagc ggaccaccaa
    18301 aaaccgccgt agccaatatc ttgaaatcct ccatatttgt tacgcaagcc acccgggagg
    18361 cctgtaaaac cggtctcgtt ggtggatccg ggatttgaac ctgcccaatg cccggttccg
    18421 ctttctttga gtttatcacc ggcaatgctt tcaccaccga ggtaatcggt taactctgtc
    18481 cattccgcat ggcttggaac atgccaaccc tgcggacaca atttgcccgt ttctaccgtg
    18541 aaccaattgt atagaaggcc ataatcgctg tataaggccg ggtcattgtt gtaccggcag
    18601 tagccgggtg ttgtcagggt gttccagctt gaattgtcgg ttatatgagg tatttctgtc
    18661 ccatcgttca gaagggtgct tttcaggttt tcggccatcc aaatctgatt gcctatggct
    18721 actgttttgt aaacattgcc ttgaatatcc aggacggtat tgcctggagg attggggttg
    18781 tcgtcgctgt caaactcaat aaccgaagtc ggggtaatgg aagaccgcac tatcctgaga
    18841 ttttgggtag tttcggtcat ttgttgatga tcggtggtac gcacgatcaa ggtcactgat
    18901 tcatagaggg ccggaggaag tacaaaataa aaggctgtgg cttgctcatc atcgagttgg
    18961 acaccttcgc cgcaatcgag ggtaagggtg tcgtaagcag gcggtgtagg cattattaat
    19021 tggggcaccc cctggtagcg catatcgact ccggccaccc cggccagtcc gggagcacaa
    19081 aggcttagag aacttacgga cacgtctcca catagattca gcttgagcag gccgcaaaga
    19141 tttttaaaat gcaagttggt atcgtaactc caggaaacca tggggtaagc tccactggca
    19201 aaaccgccgg ccttatattg ttgcgtatag ggtaatttca gtgtcagcaa agtatctctg
    19261 ttcaggttga gggcaagttt ccagggataa aaggcaacat acataccggc atcgatgaga
    19321 tcaccgctga aagtagcgta aggcaaggtg tcgctgctta tgttgacaaa aggcttaaac
    19381 tcacccaaat cggcggaaaa agcaatgctg tcgaggggaa gaaagcaaat gctgcgcaca
    19441 gggtcgccgg gttctccgct aagcagggtt ttggtctgag cgttttccgt cgaggcatga
    19501 atgctcattg gggaattttg atgcttgccg tcccgggaag aaaacacatc accggtacat
    19561 cccatcagca acaataaaac tggaaaacaa ggaataaacc tatgtgaaat ggtcttcata
    19621 tttattatct aagatctaag tatatgacaa aaatttgaaa acattgattc gatatcggtt
    19681 tgtatgattg a
//
LOCUS       DAOSTD010000030        19660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_2934_length_19660_cov_10.231370, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000030 DAOSTD010000000
VERSION     DAOSTD010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19660
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2934_length_19660_cov_10.231370"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /gene="cysS"
                     /locus_tag="PLI37_03330"
     CDS             complement(<1..968)
                     /gene="cysS"
                     /locus_tag="PLI37_03330"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOQ58082.1"
                     /translation="MMQQLSIYNTLSRQKEVFKPLHQGRVGLYVCGPTVYGDPHLGHA
                     RPAITFDILFRYLSRLGYKVRYVRNITDVGHLENDADEGEDKIAKKARLEELEPMEVV
                     QYYLNRYHKAMEALRVLPPSIEPHASGHIIEQIRMVQKILDNGYAYVSKGSVYFDVEK
                     YNKSHHYGKLSGRNIEDMLSATRSLEGQDEKKNAMDFALWKKASPEHIMRWPSPWSDG
                     FPGWHIECTAMGTRYLGEEFDIHGGGMDLLFPHHECEIAQSVAALNKESVRYWMHNNM
                     ITINGQKMGKSLGNFITLEEFFNGSHPMLTQAYAPMTIRFFILQAH"
     gene            complement(953..2029)
                     /locus_tag="PLI37_03335"
     CDS             complement(953..2029)
                     /locus_tag="PLI37_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOQ58083.1"
                     /translation="MSDFFTDIAQLSFKTRLEELKDYAFASYNIQNEQMFNLLNEAQA
                     CFYGRKYNFRSIFSYQDFRERLPILKNSDIQPYLRKMTAGKPNILWPGVCKHFVEVPQ
                     SGGQKSGNDKYYLPVSDQMLSENFFQGINDCYALYLRDYPESRLFSAFSVWLGVDSKE
                     ENLGNITSVLRKNMPFIMSLLTFPNELPEESADEQLTEKIFKKCQQDKISNFHGTLSS
                     FRGFLDEASAFFGKKSIREICPSAEVLFHRGIPLSAQMEQGVEGLNYQASYCSPEGFF
                     GIQERPGEAALLLMIDISIFYEFIPVDVALEPENAIPLEEVVLGKDYRMIITTCSGLW
                     RYCSGGPAVRFVSEKPYKFILCNN"
     gene            complement(2102..2911)
                     /gene="rnc"
                     /locus_tag="PLI37_03340"
     CDS             complement(2102..2911)
                     /gene="rnc"
                     /locus_tag="PLI37_03340"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOQ58084.1"
                     /translation="MGPGRITFPFFFNKAFYKKENRESYSVFRKILGFYPRNLVLYEL
                     AFRHKSASIRGRDGVLLNNERLEFLGDAVLNVLVTDLLFSHFECEGEGFLTNTRSKIV
                     QRETLNNIALELGLDKWVLKAEYASSPRQSIFGNSLEALIGAVYLDRGYRSCKRFLKK
                     KIIQRFIDVEHIANQEINFKSKIIEWCQHQKVELNFEHIELLAEEESPLNFEARLFLN
                     GIYVASGRGCTKKQAQQKAAQDAIATVESRQGLYADIMAAGIRQKMQENYF"
     gene            complement(2893..4158)
                     /gene="fabF"
                     /locus_tag="PLI37_03345"
     CDS             complement(2893..4158)
                     /gene="fabF"
                     /locus_tag="PLI37_03345"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOQ58085.1"
                     /translation="MELKRVVVTGLGAITPLGNSVSEYWTGLLNGVSGSGPISRFDTT
                     HFKTKFACEVKNYNPEDYFDRKEIRVYDHFAQYAIISAKEAISDARLIPEQEDLDRIG
                     VIWGAGIGGIQSFENEVTDFAKGDGKPRFSPFFVPRMIADLSAGHISIMYGFRGPNYC
                     TVSACASSNNAFFDAFNYIRLGKANVIVCGGSEAAITPCGVGGFNAMRAISTRNEDYA
                     TASRPFSKSRDGFVLGEGGACLIFEELEHALARKAPIYAEVAGCGLSADAHHITAPHP
                     DGLGATKVMENALEDAGMQPEDIDYINVHGTSTPLGDIAEVKAVKKIFGEHTYKLNIS
                     STKSMTGHLLGAAGAIESVACIKAIQENIIPPTINFTEGDEDPEIDYGIDFTFGKACK
                     REVRAALSNTFGFGGHNASIIFKKWDPGV"
     gene            complement(4227..4466)
                     /locus_tag="PLI37_03350"
     CDS             complement(4227..4466)
                     /locus_tag="PLI37_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOQ58086.1"
                     /translation="MSETADKVKSIIAEKLGIELSQVTPTASFTNDLGADSLDTVELI
                     MSFESEFGISIPDEEAEKISTVSDAIDYVEKTLKK"
     gene            4618..5196
                     /gene="purN"
                     /locus_tag="PLI37_03355"
     CDS             4618..5196
                     /gene="purN"
                     /locus_tag="PLI37_03355"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOQ58087.1"
                     /translation="MKNLQKHRLAVFASGSGSNAENIARYFDGREDVKVVLFLSNKKN
                     AYVLERGKKLRIASHAFEKTDMEEGRILQLLQENQVNFIVLAGYLQKIPPEIIQAYPD
                     KIVNIHPALLPKFGGKGMYGERVHEAVIQAGEKESGITIHYVNEHYDRGNIIFQASCP
                     VLASDTPSELALRVHELEYLHYPRIIDKLLKE"
     gene            complement(5315..6850)
                     /locus_tag="PLI37_03360"
     CDS             complement(5315..6850)
                     /locus_tag="PLI37_03360"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOQ58088.1"
                     /translation="MKNVIKVSIGRWAFTLNEEAYILLGNFLDRLKAYYEKEADTDKR
                     VQEIENLIGEYLSDCVKSIDQVVDEKQIQQAIELLDLPDFGQQAASTDGSAAESDNCG
                     RSQKRLYRSAERKVLGGVFSGLGEYFGIDPVGLRLLYVVFVVIGMFSFRFNMGDHIHF
                     RLFGIGGITSFLLLMYLVMWIVMPLARTPQEKRAMRGGRTSARRVVHDLGTQPGSNYR
                     EDRNFSLGSFLLSFIRVFVGVVLILVGASGLLALPMLLYFWDMPADWFGYLELLPLRP
                     GLLWFKVLTLIAVFVPLLLFLYEGIVLLLKLRVRKFNLGAIFLVVWILSLFILAFSGL
                     LASRSFWGEGKYQSTEALDLPSDTLYLEIDAEFSSPEQFVCAYEDEFFVYCSKSSSDD
                     KFLYIIPQVRLSSGDTPKMTQGVSLRAKNNIIAQIKAEDCVDFVKQEGNKIKVSPLYF
                     DKDKPWTFEQMSLHLFLPENAVLYVQGKKGKWQEIRHNRGSFYPFVGYTDDIEGFIIS
                     NSF"
     gene            complement(6847..7182)
                     /locus_tag="PLI37_03365"
     CDS             complement(6847..7182)
                     /locus_tag="PLI37_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOQ58089.1"
                     /translation="MNTDNVKAQMRKGVLEYSILSILSRGDEYASSLIKILKDARMIV
                     VEGTLYPLLTRQKNQGLLSYRWEESPQGPPRKYYSITDKGREMLSELDVTWKEIVDSI
                     EIIRNTPNQ"
     gene            complement(7191..7760)
                     /locus_tag="PLI37_03370"
     CDS             complement(7191..7760)
                     /locus_tag="PLI37_03370"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOQ58090.1"
                     /translation="MKKVVSKGIGGYSFQIEEDACRILQDYIEQFKSKLGEAEDVSEI
                     MDDIEARIAEILREEIHSPNQVVGEGLIRQVVARMGMPDGSNDFTEYHVPYTSTKRAT
                     RRLFRNPDDRKIGGICGGLGAYLGVDATLLRILFLIGFFLGLLSFWVYLILWICVPDA
                     RTPAQKLQMQGLPVTAENLLRYSAKQSEK"
     gene            complement(8063..9364)
                     /gene="tyrS"
                     /locus_tag="PLI37_03375"
     CDS             complement(8063..9364)
                     /gene="tyrS"
                     /locus_tag="PLI37_03375"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOQ58091.1"
                     /translation="MNFIEELSWRGMIHDMMPGTEEQLKKEMTTAYVGIDPTADSLHI
                     GHLVGIMMLRHLQRSGHKPIALIGGATGMIGDPSLKSGERNLLDEETLRHNQEGIGRQ
                     LAKLLDFESTAPNAAELVNNYDWMKDYSFLNFIRDIGKHITVNYMMAKDSVKKRLSAD
                     SNVGMSFTEFSYQLLQGYDFLYLNEHKNCKLQMGGSDQWGNITTGTELIRRKNGGEAY
                     ALTCPLITKADGGKFGKTESGNIWLDPRYTSPYKFVQFWLNVSDADAEKYIKIFTFLG
                     REEIESLCVQHNKAPHLRLLQKKLAEEVTCMVHSREEYEAATEAAAILFGSSSTEQLL
                     RLDEKTFLDLFEGIPVFQVDPDLFKQGVKAVDLLAEHAAVFPSKGEMRKMVQNGGLSI
                     NKNRCQQFDQLLDTSFLLHGRYLLIQKGKKNHFLITTSSEN"
     gene            9569..10252
                     /locus_tag="PLI37_03380"
     CDS             9569..10252
                     /locus_tag="PLI37_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ58092.1"
                     /translation="MKTLRNSALIFATKPSAITPQIEEFLKDRKAYFETTNNVQKALD
                     FITTDSPCLVIFDTPTDDPKALDLCKKLKKLKEESRFNIYFLIDNNSNLEEQLDWFKN
                     GVDEFGTRDTNSILICQRIRNILAKYEDSLIDVIPQKGIVIDSDRYLVYKNGKEIFLP
                     RKEFELLQLLNSRPTKVFTRDEIFRHVWGNRSIVGDRTIDVHVRKIREKIGSNHIVTV
                     KGVGYKFIG"
     gene            complement(10242..10928)
                     /locus_tag="PLI37_03385"
     CDS             complement(10242..10928)
                     /locus_tag="PLI37_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOQ58093.1"
                     /translation="MFVDIHTHREQHYLKFIEQEQFDSLSPRRDNSRWFFLNIASEDF
                     IPGSNLPPFLSLSTGIHPWKADVLPDTEKLEAVFAHPDIRLIGEIGLDKRCMVPYELQ
                     FQVFELQLEMAGTLKKPVLIHQVSSMDKILVCKKAHPEIPAWIIHGFRGKKTQALQWL
                     QHDFFLSFGPQFNNAALQACPPERLFFETDASGIAIEEIYKKAAQVFACPVTALELQV
                     ERNVNSLFNQ"
     gene            complement(10949..11170)
                     /gene="yidD"
                     /locus_tag="PLI37_03390"
     CDS             complement(10949..11170)
                     /gene="yidD"
                     /locus_tag="PLI37_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOQ58094.1"
                     /translation="MRKLVIFLVLLPVRFYRLFISPLIPSSCRYTPTCSEYALQAVQK
                     YGPVKGVRLAIRRILRCHPWGGHGYDPVP"
     gene            complement(11167..11559)
                     /locus_tag="PLI37_03395"
     CDS             complement(11167..11559)
                     /locus_tag="PLI37_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOQ58095.1"
                     /translation="MDKSQLSFGKAERLCGELRINKLFEQGKSFVVYPLRVCWLLVPP
                     AEKAPVQVMVGVPKKKLRKQVQRNRVRRLIRETYRLSKGDLLNEVRGKEAQLLLAFIW
                     MSEQVPEYKDLKTKMQEALRQLRKKLSL"
     gene            complement(11654..12403)
                     /locus_tag="PLI37_03400"
     CDS             complement(11654..12403)
                     /locus_tag="PLI37_03400"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005682536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOQ58096.1"
                     /translation="MKIKKILVSQPQPATEKSPYFDIEKKYGVKFVFRPFIKVEGISA
                     KEFRQQKIAIQDYSAVVFTARTAIDHFFRLCEELRVSVSEELKYFCIGEQIALYLQKY
                     IQYRKRKIFYPESGRVEDLVELIQKHSNDNNFLVSVSYVHKDDLISKLDENNINYTKA
                     VMYRTVSNDFEEGEAFDYDMLIFFSPSGIVAMLKNFPNFVQGDIHIACLGPTTARAIR
                     EAGLRLDLEAPTPNAPSITAALDQYLKDNGK"
     gene            complement(12422..13210)
                     /locus_tag="PLI37_03405"
     CDS             complement(12422..13210)
                     /locus_tag="PLI37_03405"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HOQ58097.1"
                     /translation="MAVQDYVRSESFDYPEAPDSLAADVTENSLTLQRPLYEGIPRQE
                     GPLFGLKPFIVLILGLFVFSYIFGPRSSLFLHSLKDIFVYRERSSIFVDSDVRLVRYR
                     RLLSVFGFLVCLLLLSLIFQPEQNASLNSLDARSLGYALLFLGVFLLAKFLLYRFFTS
                     FHFDVSTARRWQNDNLILFIVLSVSYAILVFVNEIVLIGPECLLKIALILWILDKLIL
                     LAKTVHYFSDQRLSWFHFIIYLCALEILPASIFYKGIIWVRGIV"
     gene            complement(13217..13972)
                     /locus_tag="PLI37_03410"
     CDS             complement(13217..13972)
                     /locus_tag="PLI37_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOQ58098.1"
                     /translation="MEKTININLRVWRQRGPKAKGAFESYKLDGVSTDSSFLEMLDQL
                     NEQLISEGKEPIVFDHDCREGICGMCSLYINGHPHGPDEDITTCQLHMRKFKDGETIT
                     IEPWRSHGFPVIRDLMVDRSAFDKIIQAGGYVSVNTGGIPDANAIPIPKPVADMAMDA
                     AACIGCGACVAACKNGSAMLFVAAKVSQLALLPQGKVEAARRAKAMVQKMDELGFGNC
                     TNTGACEAECPKNISITHIARLNREFLKAKFKD"
     gene            complement(13974..15929)
                     /locus_tag="PLI37_03415"
     CDS             complement(13974..15929)
                     /locus_tag="PLI37_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOQ58099.1"
                     /translation="MSTDKKTKTIVAKIPEGALSEKWSNYKAQQKLVNPSNKRRLDVI
                     VVGTGLAGASAAAALGELGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYR
                     LFYDTIKGGDYRAREANVYRLAEVSNSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVS
                     RTFYARGQTGQQLLLGAYSALSRQVHKGSVKLYTRREMLDLVVVDGKARGIIVRNLIT
                     GKIERYSAHAVVIGTGGYGNAFFLSTNAMESNGSAAWQVYKKGAYFANPCYAQIHPTC
                     IPVQGEMQSKLTLMSESLRNDGRIWVPKKKEDAQAVRAGKLKPTEIAEEDRDYYLERR
                     YPAFGNLVPRDVASRAAKERCDAGYGVGSGLAVYLDFKDAIERLGKDVVETRYGNLFQ
                     MYEKIVDDNPYETPMMIYPAIHYTMGGIWVDYELQTTIPGLYSIGEANFSDHGANRLG
                     ASALMQGLADGYFVLPYTIQNYLADEIRTPRISTDLPEFAEAERNILERIERLMSIKG
                     KRSVDSLHKQLGHIMWDFVGMGRNKKGLLKAIDEIKQVKKEFWSNVFIPGEGLDLNVE
                     LEKALRLADFIEIGELMARDALHREESCGGHFREEYQTPEGEALRQDDKFNYVACWKY
                     MGEDKEPEMYKEELKYEFVQVQQRNYK"
     gene            complement(15937..16617)
                     /locus_tag="PLI37_03420"
     CDS             complement(15937..16617)
                     /locus_tag="PLI37_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOQ58100.1"
                     /translation="MAVFIINSSIGRKLIMSITGAALVLFLTFHSIMNVFVIISPKVY
                     DAICYFLGANWYALIATLVLAFLTVVHVLYAFWLTLQNKIARGRDAYSVNKRPKGVEW
                     ASENMLVLGLIILGFLALHLYNFWYKMQLTELLGKINGEEISVAGAALVQGLFQSPVY
                     CLLYIVWLTALWFHLTHGVWSALQSIGWSNDIWLPRIKVISNVVSTIVVGLFMLVPIY
                     YLIVNFIL"
     gene            16781..17677
                     /locus_tag="PLI37_03425"
     CDS             16781..17677
                     /locus_tag="PLI37_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOQ58101.1"
                     /translation="MNTNQSKRLKETICQLSQYESFKELRHERAYSDTLPSTIKLEEI
                     IRICRGILFPGYFGNASVNKQNLAYHMGLNTEKLLVLLSEQIAAGLSFFPRDKYQNAT
                     ELKEKADTIAEEFIFNLPELRKILYTDIMATYNGDPAAESIDEVIYCYPGIRAISSYR
                     IAHLLLKLDVPIIPRIITEMAHSETGIDIHPAASIGHSFSIDHGTGVVIGATAILGNH
                     VKLYQGVTLGAKSFSLDDKGNPVKGVPRHPILEDNVIVYSNATILGRITIGENAVVGG
                     NIWVTEDVPPGARVVQSKRISE"
     gene            17684..18838
                     /locus_tag="PLI37_03430"
     CDS             17684..18838
                     /locus_tag="PLI37_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOQ58102.1"
                     /translation="MREDSFEMLAKTLQGLEGVLAEEIVKLGGNNVKPAKRSVFFEGD
                     KSLMYKANLRLRTALRILKPIAVFDAAHPDQIYEAVKAVNWKNFMDADQTFMVETVVY
                     SDTFRHSNFVSYRVKDAIADHFREKTGKRPSVQLVNPDLYIHLHIARKHCTLLLDSSG
                     ESLHKRGWRIKQTEASLNEVLAAGMLLLAGWKGQCPLTDPMCGSGTLLIEAALIALDI
                     PPGIYRRHFAFEKWKDFDRELFRTLYEEENRKEFRFRIQGNDISARAIKMAAENIKNA
                     GLSKHIDLKVQGVQEITQAPPNGLLVCNPPYGERLDDNKLNELYQSLGERLKHVFAGN
                     TAWILSSRKDLLNCLGLRHSARHFLMNGALNCEYRRYDIFSGKKKDQFTQ"
     gene            18853..19476
                     /locus_tag="PLI37_03435"
     CDS             18853..19476
                     /locus_tag="PLI37_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HOQ58103.1"
                     /translation="MAKKNKFYVVWQGRKPGIYSSWEACKSEVEAFESARYMGFETQE
                     QAERAFKDGWKKHYALKKSKGGNPEPQTRPTGEALTVDAACSGNPGKMEYRGVLLHNG
                     KEVFHEGPFEEGTNNIGEFLAIVHALALLDKRNIRLPVYSDSANALLWVKKKKCGTKL
                     LPSPRNKELFQLINRAEQWLLKHDRDFPVYKWDTKAWGEIPADFGRK"
     gene            complement(19481..>19660)
                     /locus_tag="PLI37_03440"
     CDS             complement(19481..>19660)
                     /locus_tag="PLI37_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58104.1"
                     /translation="YNRTDAFVYLISTKRVNCFLSFNFHYTRQGGLNHQQQLVLRFNP
                     GNMLPVEKSMSVLGK"
BASE COUNT         5587 a         4679 c         4170 g         5224 t
ORIGIN      
        1 taatgagcct gtaaaataaa aaagcgaatg gtcattggag cgtaggcctg cgtcagcatg
       61 ggatggctgc cgttgaaaaa ttcttccagt gtaatgaaat ttccaagcga cttgcccatt
      121 ttttgcccat tgatggtaat catattgttg tgcatccaat agcggacact ttctttgttg
      181 agggctgcca ccgattgggc tatttcgcat tcgtggtgag gaaaaagcaa atccattcca
      241 ccgccgtgaa tatcgaattc ttctcccaga tagcgggtgc ccatagcggt acattctata
      301 tgccaaccgg ggaaaccatc gctccagggc gaaggccacc gcattatatg ttcgggcgag
      361 gccttcttcc acaaagcgaa gtccatagcg ttcttttttt cgtcctgacc ttcgaggctg
      421 cgggtggcac taagcatatc ttcgatgttg cgtcccgaca acttgccgta atgatggctc
      481 ttgttgtatt tttcaacatc aaaatagaca gagcctttgc tcacgtacgc ataaccgttg
      541 tcgaggattt tttgcaccat ccgtatctgc tcaataatat gccccgatgc atgaggctca
      601 atgctgggag gcaaaacccg gagtgcctcc attgctttgt ggtagcgatt taaataatac
      661 tggaccacct ccatgggttc gagctcttcc agccgggcct ttttagcaat tttatcctct
      721 ccttcgtctg catcgttttc caaatggcct acgtcggtaa tgttgcgaac gtagcgcact
      781 ttatagccca accggcttaa gtagcgaaat agtatatcga aggttatggc cggacgggca
      841 tgacccagat gcggatcgcc gtaaaccgta gggccacata cataaagccc cacacggcct
      901 tggtgcaggg gtttgaaaac ttctttttgc cggcttaatg tattgtaaat actcagttgt
      961 tgcataatat aaatttgtag ggcttctccg aaacaaagcg aacagccggg ccaccggagc
     1021 aatatctcca caatccggag caagtggtaa taatcattct atagtctttg cccaggacga
     1081 cttcttccaa aggaattgca ttttcaggtt caagagcaac gtcgaccggt ataaattcgt
     1141 aaaaaatact gatgtcgatc atcagtaaaa gggcggcttc gccgggccgt tcctgaatgc
     1201 cgaaaaagcc ttcgggactg caatatgagg cctggtaatt caatccttct acgccctgtt
     1261 ccatttgagc ggagaggggg atacccctgt gaaagagcac ctcggccgag ggacagattt
     1321 cccgaatgct ctttttgccg aaaaaagcgg aagcttcgtc caaaaagccc ctgaacgaag
     1381 aaagtgttcc gtgaaaattg gaaattttat cctgctgaca tttcttaaaa attttctctg
     1441 tcagctgttc gtcggccgat tcttcgggca attcattggg aaaagtgagc aagctcatta
     1501 taaagggcat attcttacgt agaacagacg tgatattccc caggttttct tctttgctgt
     1561 ctactcccaa ccacaccgaa aaggcggaga aaagcctact ctccggataa tccctcaaat
     1621 acagtgcata gcaatcgttg ataccctgaa agaaattttc gctcagcatc tgatcgctga
     1681 caggcaagta gtatttatcg tttccggatt tttgacctcc gctttgagga acttccacaa
     1741 aatgtttgca aacgcccggc cacaggatgt tgggtttgcc ggcggtcatt ttgcgcaaat
     1801 aaggctgaat atcggagttt ttcagaatag gaagcctttc gcggaaatcc tgataactga
     1861 aaatgctcct aaagttgtat ttcctcccat aaaaacaagc ctgagcctcg ttcagcaagt
     1921 tgaacatctg ctcattctgg atattgtaag acgcaaatgc gtaatctttc aattcttcca
     1981 gtctggtttt gaatgataac tgggcaatgt ctgtgaaaaa atcactcata acacaggggg
     2041 cttgaatatt agcctcaaaa gtaatgcatt tttccacagc attcaagccg ggaataaaat
     2101 ttcaaaaata attttcttgc attttttgcc ggatgccggc agccataata tcggcataca
     2161 aaccttgccg gctttctaca gtggcaatcg catcctgagc tgctttttgc tgggcctgct
     2221 ttttggtaca tcccctgccg ctagccacat aaatgccgtt caaaaaaaga cgggcttcga
     2281 agttgagcgg cgattcctcc tcggcaagca gctcaatatg ttcaaaattt aattcaactt
     2341 tttgatgctg acaccactca ataattttcg atttgaagtt gatttcttga ttggcaatat
     2401 gttcaacatc aatgaaccgc tgtatgattt tctttttgag aaaacgcttg caactgcggt
     2461 aacctctatc caaatagacg gctcctatga gagcttcgag actattccca aaaatggatt
     2521 gcctgggaga tgaggcatat tcggctttga gtacccattt atccaaaccc agttccagag
     2581 ctatgttgtt tagggtttcc cgctgtacga tttttgaacg agtgttggta agaaaaccct
     2641 ctccctcgca ttcaaaatga ctgaagagta aatcggttac caacacattc aacacggcat
     2701 ctccgagaaa ctcaagacgc tcattattga gcaaaacgcc gtcgcggcct cttatcgaag
     2761 ccgacttgtg tcgaaaagcc aactcgtaca aaaccaaatt acgaggataa aacccgagaa
     2821 tctttctaaa aacagaatac gattccctgt tttccttttt ataaaatgcc ttgttgaaaa
     2881 aaaacggaaa agttatacgc ccgggtccca ctttttgaaa ataatgctgg cattatgtcc
     2941 cccaaatccg aaggtattgg acaaagctgc gcgaacttcg cgtttacagg cttttccaaa
     3001 ggtaaaatca atgccgtaat cgatttcggg atcttcgtct ccttcggtaa aattgatggt
     3061 gggaggaatg atgttttcct gtatggcctt gatgcaggca acagattcta tcgctccggc
     3121 cgcacccagc aggtgtccgg tcattgattt ggtggaactg atgttcaatt tatacgtgtg
     3181 ttcaccgaat atttttttga cagccttcac ttcggcgatg tcacctaagg gagtggaggt
     3241 tccgtgcaca ttgatgtagt cgatgtcttc gggctgcatt ccggcatcct ccagcgcatt
     3301 ttccattact tttgtggcac ccaggccatc gggatgaggt gccgtaatgt ggtgagcatc
     3361 ggccgataat ccacaaccgg ccacctcggc ataaatagga gccttacggg ccagtgcatg
     3421 ttccagctct tcgaaaatca ggcaagctcc tccctcgccc agcacaaaac catcgcggct
     3481 tttgctgaag ggacggctgg cagtggcgta atcttcgttg cgggtagaaa tggcacgcat
     3541 ggcattgaat ccacctaccc cacagggagt aatggcagcc tcggaacctc cgcaaacaat
     3601 aacattggct ttacccaaac gtatgtaatt gaaagcatca aaaaaggcgt tattcgaaga
     3661 ggcgcatgcc gatacggtgc aataattcgg cccccggaag ccatacatga tcgaaatatg
     3721 acctgccgac aaatctgcaa tcatccgggg aacaaaaaac gggctaaacc tgggtttccc
     3781 gtctcctttg gcaaaatcag tcacctcatt ttcaaaactt tgaatacctc ctattccggc
     3841 gccccagata acaccaattc tgtccaaatc ttcttgctca ggaattaagc gagcatcgga
     3901 aatagcttct tttgcagaaa ttatggcgta ctgtgcaaaa tggtcgtata cgcgaatctc
     3961 tttgcgatcg aaataatctt ccggattgta attctttacc tcacaggcaa attttgtttt
     4021 gaaatgtgta gtgtcaaaac gtgaaatagg cccggaacca cttactccat taagtaaacc
     4081 cgtccaatat tctgatacgg aattacccaa aggggtaata gctccaaggc ctgttacgac
     4141 aactcttttt aattccattt tctaggaatc ttcaagaagt cccgtgttcg gttttcctct
     4201 tatgtaccgg gaactcctgt atggaattat tttttcagag ttttttcaac gtaatcgatt
     4261 gcgtcactga ctgttgagat cttttcagct tcctcgtcag gaatactaat gccgaattcc
     4321 gattcgaaac tcataatcaa ctctacagtg tcgagagagt cagcaccaag atcattagtg
     4381 aagcttgcag taggggtaac ttgagaaagt tcgatgccta atttttccgc aataatcgat
     4441 ttaactttat ctgcagtttc agacataaca gttaattttt tagtgaataa ataagtttgt
     4501 ctcaaaaaaa ttgagacgac aaaggaatga aatatttcaa acaaacgcta attttgtgct
     4561 gtaattattc taaaaactcc tttcgctttt taacatattg ttgaatatct gtcaattatg
     4621 aaaaacctcc aaaagcatcg cctggccgtt tttgcctccg gttccggctc taacgccgaa
     4681 aacatagccc gctatttcga tggaagggaa gatgtgaaag tcgtattatt cctttccaac
     4741 aaaaagaatg cctacgtact cgaaaggggg aaaaaactcc gtattgcctc ccatgctttt
     4801 gaaaaaacag acatggaaga gggaagaatc ctgcagctgt tgcaggaaaa tcaagtaaac
     4861 tttattgttc tggccggcta tcttcaaaaa attccgcctg aaattattca ggcttatccc
     4921 gacaaaatag taaacattca ccccgcatta ttacccaaat tcgggggcaa gggcatgtat
     4981 ggcgaacggg tacacgaagc agttatacag gcaggcgaga aggaatccgg cattaccatc
     5041 cactacgtaa atgagcacta cgatcgggga aacatcattt tccaagccag ttgtccggtg
     5101 ttggcttcgg atacgccttc ggagcttgca ctacgcgtac acgaactgga atacctgcac
     5161 taccccagga ttatagacaa actattgaaa gaataatcat tgcatcttta tatgataatt
     5221 ggtatctctt gaatgagcat tatctaaaaa atcctccgga agcatgggct ggttccggag
     5281 gattgataag tcgatccgat tgatgctgtt ttacttaaaa tgaattacta attataaagc
     5341 cttcaatatc atcggtatag cccacaaaag gatagaacga acctctgtta tgccttattt
     5401 cctgccattt accctttttc ccctgtacat acaatacagc attttcgggc aaaaacaaat
     5461 gcaggctcat ttgttcgaaa gtccagggtt tatctttgtc aaaatataaa ggactgactt
     5521 taattttgtt tccttcctgt ttcacaaaat caacgcagtc ttcggcctta atttgggcaa
     5581 taatgttgtt tttagccctt agactcaccc cttgggtcat tttgggcgta tcaccgctgc
     5641 tcaggcgaac ttgaggaata atatagagaa atttgtcgtc ggagctgctt ttgctacaat
     5701 aaacaaagaa ttcatcttcg taagcacata caaattgttc aggcgaagaa aattcggcat
     5761 ctatctcgag gtataaggta tctgagggca aatccaaggc ttctgtgctc tgatattttc
     5821 cctcacccca aaaagaacgg ctggcaagta aaccgctgaa agcaagtata aaaagagata
     5881 atatccaaac gacaagaaaa atagctccca agttaaattt acgcacccgc agtttaagca
     5941 acagaactat tccttcgtac aagaacaaca acaaaggcac aaaaacggca attaatgtca
     6001 ataccttgaa ccagagcaaa cccggacgca gaggcaataa ctccagataa ccaaaccaat
     6061 cggcaggcat atcccaaaaa tacaacaaca taggcaaggc aagcaaaccg gaagctccaa
     6121 ccaaaattaa aacaacacct acaaaaacac ggatgaagct gagcaaaaaa ctgcccagac
     6181 tgaaatttct gtcttcgcgg taattgctgc cgggctgagt ccctaaatca tgaacaaccc
     6241 tgcgggccga agttcgtcct cccctcatgg cccttttctc ctggggcgtt cgagccaaag
     6301 gcattacgat ccacataacc aaatacatca aaagaagaaa agaagtaatg cctcctattc
     6361 caaacaggcg gaaatgtata tgatcaccca tgttaaaacg gaaactaaac attccgatca
     6421 caacaaaaac cacgtacaag agacgtaagc caaccggatc tataccaaaa tattcgccca
     6481 gtccgctgaa cactccgccc agcacctttc gttcagcact acgatacaag cgtttttgac
     6541 tcctgccgca attatcgctc tctgccgcgc tgccgtctgt cgaagcagcc tgttgtccaa
     6601 aatcgggtaa atccagcaac tcgatggctt gctgtatttg tttctcgtcc accacttgat
     6661 cgatagattt aacgcaatcc gataaatact ccccgataag gttttcaatc tcctggacgc
     6721 gtttgtccgt atcggcttct ttttcataat aagccttgag ccggtccaaa aagtttccca
     6781 gcaaaatata ggcctcttcg ttcaaggtaa aggcccagcg accaatactt actttaatta
     6841 catttttcat tgatttgggg tattacgaat tatttctata ctatcgacaa tttccttcca
     6901 ggtcacatca agttccgaga gcatttccct acccttatcc gtgatgctgt aatacttgcg
     6961 gggaggcccc tgaggcgatt cttcccaacg gtaactgagc aaaccctggt tcttttgtct
     7021 ggtaagcagg gggtacaaag tgccctccac cactatcatc cgggcgtctt taagaatttt
     7081 gatcagggaa gaagcgtatt catctcctct ggacaagatg gatagaatac tatactccag
     7141 aactcccttt ctcatctgag ccttaacatt gtccgtattt atttcccggt tcatttctct
     7201 gattgtttag ctgaataacg caataaattt tcggccgtta ccggaagtcc ctgcatctgc
     7261 agtttttgtg caggtgtcct tgcgtccggc acacatatcc acaaaataag gtatacccaa
     7321 aacgacaaga gccccaaaaa gaagccaatc aaaaacaaaa tcctcaacag ggtggcgtcc
     7381 actcccaaat aggctcccaa acctccgcat attccaccta tcttacggtc gtcgggatta
     7441 cggaaaagcc tgcgtgtggc tctttttgtc gaagtatagg gcacgtgata ttctgtaaaa
     7501 tcattgctgc cgtcgggcat acccatgcgt gccactactt gtcgtatcag gccttcgccc
     7561 acaacttggt tgggagaatg tatttcttcc cgaagaattt cggcaatgcg ggcctcaata
     7621 tcgtccatga tttcggagac gtcttcggct tctcccagct tgctcttaaa ttgttcaatg
     7681 taatcctgca aaatgcggca ggcatcttct tcaatttgga aagaatagcc cccaatcccc
     7741 tttgaaacaa cctttttcat gagttttatt atttgattta gtccggttcc aacatcggaa
     7801 tccggtattc ggggacaaat atagtatggt gtatagtact atgcaataca tagtactaag
     7861 atttaagatt attttacgat taaaaagcct ccggcccgtt tgggaaccgg aggctttttg
     7921 gttgtctcac gaagactcga acttcgacag gcagaaccaa aacctgcagt gctaccatta
     7981 caccatgaga caatcctgtt tagctttcta aaaaagcgct gcaaagatag gtatttttta
     8041 tattaccaac caagtgtttt tattaatttt cggaagatgt agttattaag aaatggtttt
     8101 tcttgccttt ctgtatcagg agatagcggc cgtgcaagag gaaggaagta tccaaaagct
     8161 gatcaaattg ctgacacctg tttttattaa tggataagcc gccattttgt accattttgc
     8221 gcatctcgcc tttcgatggg aaaacggcgg catgctcagc caacagatca acggctttta
     8281 cgccttgttt aaataaatca gggtcaacct gaaaaaccgg aattccttcg aaaagatcta
     8341 aaaaggtttt ttcgtccaaa cgaagcaact gttcggtcga ggatgagcca aaaagtatcg
     8401 ctgccgcttc ggtggctgcc tcatattctt ccctggaatg aaccatacag gtaacttctt
     8461 cggccagttt tttttgcaac aacctcaaat gcggagcctt gttgtgttgc acacaaaggc
     8521 tttcgatttc ctcgcggccg agaaaggtaa atatctttat atatttttcg gcatctgcat
     8581 cgctcacatt caaccaaaat tgcacaaatt tatagggact ggtataacgc ggatcaagcc
     8641 agatattgcc cgattcggtc ttgccgaatt tgcctccgtc tgccttggta atcaaagggc
     8701 aggtcagggc ataggcttct cccccgtttt tgcggcgtat taattccgtc cccgtggtaa
     8761 tatttcccca ttgatcggag ccgcccatct gcaacttaca atttttatgt tcgttgaggt
     8821 aaagaaaatc gtagccctgc aacaattggt acgaaaattc cgtaaacgac atgcccacat
     8881 tggagtcggc actgagtctt tttttgacgg aatctttggc catcatataa tttacggtga
     8941 tgtgtttgcc aatatcccgg ataaaattca aaaaactgta gtctttcatc cagtcgtaat
     9001 tgtttaccaa ttctgccgca ttgggggcgg tactttcaaa atcgagtaat ttagccaact
     9061 gccggcctat tccctcctga ttgtgtctca gcgtctcttc gtccagcaaa ttacgctctc
     9121 ccgatttgag cgaaggatcg ccaatcatac cggttgcacc tccgatcaat gcaataggtt
     9181 tgtgccccga gcgctgtaaa tgcctaagca tcattatacc gaccaaatga ccaatgtgta
     9241 acgaatcggc ggtggggtcg atgcccacat aagcggtcgt catttctttt ttgagttgtt
     9301 cctctgtccc gggcatcata tcgtgtatca tacccctcca gcttaattct tcaatgaagt
     9361 tcattgttga tctaaaagta aagatgataa ttactgaggc acaaaaatac gtttattttg
     9421 tgggaaattt gaatcattct cataaagtaa cttgttttaa ttgattcttc atcttatatt
     9481 tgtgttgctt tagcaagaaa acgatattca ttgctattcg attcacttat ttttatcgca
     9541 tatatcatcc acatttaaaa caaaaacaat gaaaacgctt agaaactcgg cattgatttt
     9601 tgccacaaaa ccgtccgcca ttactcctca gattgaggaa tttttgaaag atcgcaaagc
     9661 ttattttgaa acaacaaaca acgttcagaa agccttggat tttatcacca cagactctcc
     9721 ctgtctggtc atttttgaca cgcccaccga cgatcccaaa gcattggatc tgtgcaaaaa
     9781 acttaagaaa ctcaaggaag aaagccgctt caacatctat ttccttatcg acaacaacag
     9841 taatctggaa gaacagctcg attggttcaa gaacggggta gatgaattcg gtaccagaga
     9901 caccaacagc atccttatct gccagcgcat caggaatatc ctggccaaat acgaagacag
     9961 cctcatcgat gttatacctc aaaaaggaat tgtcatcgac agcgatcgct atctggtata
    10021 caaaaacggc aaagaaattt tcctgcctcg caaagaattt gaattactcc agctcttaaa
    10081 ttcaaggccg accaaagtgt ttacccgcga cgaaatcttc agacacgtct ggggtaatcg
    10141 ttctatagtg ggcgacagaa ccattgacgt acacgttcgc aagattcgtg aaaaaatcgg
    10201 cagtaatcac attgttaccg ttaaaggagt aggctacaag tttattggtt aaacaagctg
    10261 tttacatttc tttctacctg taattcaaga gccgtaacag ggcatgcaaa aacctgtgcg
    10321 gctttcttgt atatctcttc tatggctatc cccgacgcat cggtctcaaa aaacaggcgc
    10381 tccggcggac aagcctgcaa ggctgcattg ttgaattgag gaccgaaaga aagaaaaaaa
    10441 tcgtgttgaa gccattgcaa agcctgcgtt tttttccccc taaaaccgtg aataatccaa
    10501 gctggaattt cgggatgggc ttttttgcat accagaatct tgtccatact acttacctga
    10561 tgaataagta cgggcttttt tagcgtcccg gccatttcca gttgcagctc gaaaacctga
    10621 aattgcagtt cataaggtac catgcacctt ttgtctaagc ctatttctcc gatcaggcgg
    10681 atgtcaggat gtgcaaaaac agcctctaat ttttccgtat ccggcaaaac atctgccttc
    10741 caagggtgaa tccctgtact taagctaaga aagggaggta aattcgagcc cgggataaaa
    10801 tcttcggatg ctatattaag aaaaaaccac ctggaattat ccctgcgagg cgaaagagaa
    10861 tcaaattgct cctgctcaat aaacttcagg tagtgttgtt ctctatgggt atgaatatca
    10921 acaaacataa gtattccggt ttcagtaatc agggcaccgg gtcgtaacca tgccctcccc
    10981 agggatgaca acgcaatatc cttctaatgg ccagccggac ccctttgaca gggccgtatt
    11041 tctgcacggc ctgcagggca tactccgagc aggttggagt atagcggcag gaagaaggaa
    11101 tcaggggaga aataaacagc ctgtaaaacc gtaccggcaa aaggaccaaa aaaattacca
    11161 gctttctcat aacgatagtt tttttcttaa ctgccggagg gcttcctgca tttttgtttt
    11221 caggtctttg tattcaggca cttgttcaga catccagata aatgccaaca gcagttgagc
    11281 ctcctttccc cgtacttcat tgagcagatc acctttagaa agacgatagg tttccctgat
    11341 aaggcgtctt actctgttcc gctgcacctg ttttcgtagc ttttttttgg gaacacccac
    11401 cattacctgt acgggagctt tttcggcagg gggcaccagc aaccaacaaa ccctcagcgg
    11461 ataaaccacg aacgatttcc cctgctcaaa gagcttgttg atgcgtaatt ccccacacaa
    11521 acgttccgct ttaccaaagg atagttggct tttatccata aaaaagacac tttatttctg
    11581 caaaaatagc gaaataaagt gtctttgaat taattcaaaa gaatattacc ttttactgct
    11641 actaccgttt ttgttatttt ccgttgtcct ttaaatactg gtccaaggct gcagtaatgg
    11701 agggagcgtt tggtgtgggg gcttccaggt caagcctcag tcctgcttcc cgaatggctc
    11761 tggccgtagt cggccctagg caggctatgt gaatatcccc ttgaacaaaa tttggaaaat
    11821 tcttcaacat agcaacaatt cccgaagggc tgaagaaaat caacatatcg taatcaaaag
    11881 cctccccttc ttcgaaatca ttactaaccg tacgatacat taccgcttta gtgtagttga
    11941 tattgttttc gtccagtttg gaaattaagt cgtctttgtg aacgtaagat accgaaacca
    12001 aaaagttatt gtcgttacta tgcttttgaa taagctcaac caagtcttct accctgccgc
    12061 tctcggggta aaaaatttta cgtttacggt actgaatgta tttttgcaga tagagagcaa
    12121 tttgttcccc tatgcagaaa tacttcagct cttccgaaac cgaaactctg agttcttcac
    12181 acaaacggaa aaaatggtca atggcggttc tggcggtgaa gaccacagca gaataatctt
    12241 gaatggcaat cttctgctgc ctgaactctt tggcgcttat gccctccact tttataaaag
    12301 gcctaaacac aaacttgaca ccgtattttt tctcaatatc aaaatagggg gatttctctg
    12361 ttgccggctg aggctgagaa acaaggatct ttttgatttt caacgcgata aatcgtttaa
    12421 attatacaat acctcgtacc caaataatgc ctttgtaaaa gatactggca ggcaaaattt
    12481 cgagtgcgca aaggtaaata ataaaatgaa accacgaaag tctttggtcg gaaaaataat
    12541 gaacggtctt ggccagaagt atcaatttgt ctaaaatcca gagtattaga gctattttca
    12601 ataaacactc cgggcctata agtactattt catttacaaa aacgagaatg gcatacgaga
    12661 cggagagtac aataaacaaa attaaattat cgttttgcca tcgccgtgca gtggaaacgt
    12721 cgaaatgaaa agaagtaaaa aaccggtata ataaaaactt tgccaatagg aacactccca
    12781 aaaagagtaa ggcatatcct aagctcctcg catccaaact atttagacta gcattttgtt
    12841 ccggctgaaa aatcagagat aacaacaaaa gacaaaccag gaagccaaaa accgataaca
    12901 acctccggta acgtactaat ctaacgtcgg aatcaacaaa aatactggat cgttcgcggt
    12961 atacaaagat gtcctttaat gaatgaagga agagcgagct tctgggtccg aatatatacg
    13021 aaaaaacaaa aaggcccaat atcaaaacga taaagggctt taagccgaac aggggacctt
    13081 cttgtctggg tatgccttcg tacaaaggcc tttgtagtgt cagtgaattt tcggtaacat
    13141 cggctgcaag gctgtccgga gcttccggat agtcgaacga ctcggaacga acgtaatcct
    13201 ggacagccat ggctgctcaa tctttaaatt ttgccttcaa aaattctcta ttcagacggg
    13261 caatatgggt tatggaaatg tttttgggac attcggcctc acaagcaccg gtattggtgc
    13321 aattgccaaa tcccaattcg tccatcttct gaaccatcgc cttagcccgc cgtgcggcct
    13381 ctactttgcc ctgaggcaac aaagccagtt ggctgacctt ggcagctaca aacaacatgg
    13441 ccgaaccatt tttacaggca gcgacacagg caccacatcc aatgcatgca gcggcatcca
    13501 tagccatgtc tgcaacaggt ttagggatgg gtatagcgtt ggcatcgggg attcctccgg
    13561 tatttaccga aacataaccg ccggcctgaa taattttatc gaaagcagaa cggtccacca
    13621 tcaggtcgcg gattaccggg aaaccgtggg aacgccaggg ttcgatggta atggtttcac
    13681 cgtctttgaa tttgcgcata tgaagctggc aggtggtaat atcttcatcc gggccgtgag
    13741 gatggccgtt gatgtacagg gaacacatcc cgcaaatacc ttcgcggcag tcgtgatcga
    13801 aaacaatcgg ttccttgcct tctgaaatca attgttcatt gagttgatcc aacatttcca
    13861 gaaaagaact gtcggtagaa actccgtcca gcttatagct ttcgaaagca cccttggcct
    13921 tggggcctct ttgacgccag acccttaagt ttatatttat tgtcttttcc atattatttg
    13981 taattgcgtt gttgtacttg tacgaactca tatttcaact cttccttata catttcgggt
    14041 tctttatctt cacccatgta tttccaacag gctacgtagt tgaacttgtc gtcctgacgc
    14101 aaagcctctc cttcgggggt ttggtattct tcgcgaaaat ggccgccgca ggattcttca
    14161 cgatgcagtg cgtcgcgggc catcagctcg cctatctcta taaaatcggc caggcgcagg
    14221 gctttttcca gttctacatt caaatcgaga ccttcgcccg ggataaagac attgctccaa
    14281 aattcctttt taacttgttt gatctcatca atggctttaa gcaaaccttt tttgttgcgg
    14341 cccatcccca caaaatccca cataatgtgc cccaattgct tatgcaggga atctaccgaa
    14401 cgttttccct tgatcgacat aagtcgttct atacgttcca gaatgtttcg ttctgcctcg
    14461 gcaaattccg gcaaatccgt cgaaatacgg ggtgtgcgta tttcgtccgc caggtaattc
    14521 tgtatggtat agggtaatac aaaatagccg tcggccaaac cttgcatcaa agccgaggcg
    14581 ccgaggcggt tcgctccgtg atcggaaaag ttagcttctc caatactata aagcccggga
    14641 atagtagtct gcaattcgta atcaacccag attcctccca tggtatagtg aatggccgga
    14701 taaatcatca tgggcgtttc gtaaggattg tcgtccacga ttttttcgta catctggaaa
    14761 aggttgccgt aacgggtttc aacaacgtct ttgcccaaac gctcgatggc gtctttgaaa
    14821 tcgagatata cagccagtcc gcttccaact ccgtaaccgg catcacaacg ttccttagct
    14881 gctctcgaag ctacgtctct gggcaccagg ttgccgaaag ccggatacct gcgttccagg
    14941 taataatctc tgtcttcttc ggctatttcg gttggtttta atttgcctgc acgaacagcc
    15001 tgagcgtctt ctttcttttt gggcacccaa atgcggccgt cgttacgtaa ggattcggac
    15061 atcagggtaa gtttggactg catttccccc tgaacgggaa tgcaggtcgg gtgaatctgt
    15121 gcataacagg gattggcaaa ataagcgcct tttttgtaca cctgccaggc agccgaacca
    15181 ttggattcca tggcattggt cgagaggaaa aaagcattgc cgtagcctcc tgtaccgatt
    15241 accacggcat gagccgaata tcgctcgatc tttccggtta tcaggttgcg gacaatgatg
    15301 cccctggctt tgccgtccac aaccaccaga tcaagcattt cccgccgggt atagagctta
    15361 acgctgccct tgtgaacctg acggctcagg gctgaataag ctccaagcaa cagttgctgg
    15421 cccgtttgtc ccctggcata aaaagtacgg gagacctgag ctccgccaaa ggaacgattg
    15481 tcgagcaaac caccgtattc cctggcaaaa ggaacgccct gggctacgca ttggtcaatg
    15541 atactattgg aaacctcggc caggcggtaa acgttggctt cgcgggcgcg atagtctcct
    15601 ccttttatgg tatcgtaaaa caaacgataa accgaatcac cgtcgttttg ataattttta
    15661 gcggcattga tacctccctg tgcggcgatg gagtgtgcac gacggggaga atcctgaata
    15721 cagaaattca gcacattgaa gcccaactca ccaagtgcgg ctgcagcgga agctcccgcc
    15781 aaacctgtgc ccaccacaat tacatccaga cgacgcttat tggagggatt taccagcttc
    15841 tgttgagcct tgtaatttga ccatttttca ctcaacgcac cttccgggat ctttgcgaca
    15901 attgtcttag tttttttatc ggtactcata gcccttttat agtataaaat ttacaatcaa
    15961 ataataaata ggaaccagca taaagaggcc caccactatg gtcgatacaa cattggaaat
    16021 taccttgata cgcggcaacc agatgtcgtt gctccagccg atgctttgca gggcactcca
    16081 gaccccatga gtcaggtgaa accacaaggc ggtcagccat actatgtaaa gcaggcagta
    16141 aacagggctt tgaaacaagc cctgaactaa ggccgctccc gcaacactga tttcttctcc
    16201 gtttattttg cccagcagct cggtcagttg cattttatac caaaagttgt acaaatgcaa
    16261 ggccagaaag cccaggatta tcaaacccaa taccaacatg ttttccgaag cccactcaac
    16321 acccttgggc cgcttattta ccgagtaggc atccctgccg cgcgctattt tgttttgcag
    16381 ggtaagccag aaggcataca aaacgtgcac cacagtaagg aaagccagga ccagagtagc
    16441 aatcaacgca taccagttgg ctcccaaaaa ataacagatg gcatcataca ccttgggtga
    16501 aatgatgaca aaaacattca taatactgtg gaaggtgaga aaaagcacta aggcagctcc
    16561 cgtaatactc ataatgagct ttcttccaat tgatgaatta ataataaaaa cagccatatc
    16621 cgatttaaat tagcaagttt taatctcctc gaatgagaag cggcaaaagt acgttgtttt
    16681 tgggaaaccg gcaaacaatt aaagaaatat ttccggaata ccaaaagcac ccatcaagta
    16741 aaaaaattac gtatttttgc cgaggaaata tactacaatt atgaatacta accaatccaa
    16801 aaggttaaaa gaaactattt gccaattgtc tcaatatgaa tcgttcaaag agctgaggca
    16861 tgagcgggct tattccgaca cgctaccctc gaccattaag ctggaggaaa ttatccggat
    16921 ttgccgcggc attctttttc ccggttattt tggaaacgcc tctgtcaaca aacaaaacct
    16981 ggcgtatcat atggggctta acaccgaaaa attactggtc ctgctgagcg aacaaatcgc
    17041 tgccggattg agttttttcc ccagggacaa ataccagaat gccactgaac ttaaagaaaa
    17101 ggccgatact attgccgaag aattcatttt taatttaccc gagttgcgca aaattctcta
    17161 taccgacatt atggctacct acaatgggga tcctgcagcc gaaagcatcg atgaagtcat
    17221 ttattgctat ccgggcatca gggcaatcag cagttaccgg atcgcccatc ttttgctaaa
    17281 gctcgacgtg cccattattc ccagaataat taccgaaatg gcgcattccg aaaccggtat
    17341 cgatattcac ccggcagcaa gtatagggca ttcgttttcg atcgaccacg gcacaggagt
    17401 ggttataggg gctacagcca tcctggggaa ccacgtgaaa ctttatcagg gtgttactct
    17461 gggtgccaaa agcttttcgc tcgacgataa gggtaatccc gtcaaaggag ttccccgcca
    17521 ccccatactc gaagataatg ttattgtcta ctccaacgcc accatactgg gaagaattac
    17581 tataggcgaa aacgctgtag tgggcggaaa catctgggtc accgaagatg ttcctccggg
    17641 agcacgggtt gttcaatcca aacgcatctc tgaataagca accatgcggg aagatagttt
    17701 tgaaatgctg gccaaaactc tccaaggcct ggaaggagtg ctggccgaag aaattgttaa
    17761 gttgggtgga aacaatgtaa aacccgctaa acgctctgtt ttctttgaag gagacaaaag
    17821 ccttatgtac aaggctaatc ttcgtctgag aaccgctctt cgcatactta aacccatagc
    17881 cgttttcgat gccgcccatc ccgaccagat ttacgaagcg gtcaaagctg ttaattggaa
    17941 gaattttatg gatgccgacc agactttcat ggtagaaact gtagtttact ccgacacctt
    18001 caggcattcc aattttgttt cttacagagt aaaagacgct attgctgacc acttcagaga
    18061 gaagaccggt aaacgcccct cggtgcaact ggtcaatccc gatttgtaca tccacctgca
    18121 catcgcccgg aaacactgca ctctcttact cgacagttcc ggagaatcgt tgcacaaaag
    18181 aggctggcgg ataaagcaaa ccgaagcttc actcaacgaa gtactggccg ccggtatgtt
    18241 gctgctggcg ggctggaaag ggcaatgccc tctgacagac cccatgtgcg ggtcgggtac
    18301 cctactaatc gaggcagcct tgattgccct ggatatccct ccggggatct atcgccggca
    18361 ttttgccttc gaaaaatgga aagattttga ccgggaactt ttccgcacac tctacgaaga
    18421 agaaaaccgg aaagaatttc gttttcgcat tcagggcaac gatatttcgg cacgggcaat
    18481 aaaaatggcg gccgaaaaca taaaaaatgc cggtttgagc aaacacatcg acctgaaagt
    18541 tcaaggcgta caagaaataa cacaagcgcc gcccaacggt ctgctggtgt gtaatccgcc
    18601 ttacggggaa agactcgacg acaataaatt aaatgagcta taccaatcgc tgggcgaaag
    18661 gctgaaacat gtttttgccg ggaacactgc atggatactg agttcccgca aagatctgct
    18721 caactgttta ggcctcaggc attcggcacg tcattttttg atgaatggag cattgaactg
    18781 cgaatatcgt cgttacgata ttttcagcgg gaagaaaaaa gatcagttta cccaatagaa
    18841 tagaacctgg caatggctaa aaaaaacaag ttttacgtgg tttggcaggg tcgcaaaccg
    18901 ggcatctact caagttggga agcctgcaaa tctgaagtag aggcttttga gtcggctcgt
    18961 tatatgggct ttgaaactca agaacaagcc gaacgagcct ttaaggatgg ttggaaaaaa
    19021 cactatgccc ttaaaaaaag caagggcggc aacccggagc cccaaacccg ccccaccgga
    19081 gaagcactta ccgtcgatgc agcctgcagc gggaatccgg gcaaaatgga gtaccggggg
    19141 gtcttgcttc acaacggcaa ggaagttttt cacgaaggtc cttttgaaga aggcaccaac
    19201 aacataggtg aatttctggc catagtacat gcgctggctc tgctggacaa acgcaacatc
    19261 cgtctgccgg tctattccga cagcgccaat gcactgctgt gggttaaaaa gaaaaaatgc
    19321 ggaaccaaac tcttgccaag ccccagaaac aaggaactct ttcaactgat caaccgggcc
    19381 gaacaatggc tgctcaagca tgaccgggat tttcccgttt ataaatggga tacaaaggcc
    19441 tggggcgaga ttcccgcaga ttttggccga aaataaacgc ttacttaccc aaaaccgaca
    19501 tcgacttttc caccggcagc atattcccgg gattgaacct tagcaccaac tgctgttggt
    19561 gattcaatcc tccctgccgg gtgtaatgga aattaaacga taaaaagcaa ttgacccttt
    19621 tggtcgatat caggtaaaca aaggcatcgg tgcggttata
//
LOCUS       DAOSTD010000031        18610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_3139_length_18610_cov_14.407815, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000031 DAOSTD010000000
VERSION     DAOSTD010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18610
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3139_length_18610_cov_14.407815"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(618..1043)
                     /gene="rplQ"
                     /locus_tag="PLI37_03445"
                     /pseudo
     CDS             complement(618..1043)
                     /gene="rplQ"
                     /locus_tag="PLI37_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(1048..2040)
                     /locus_tag="PLI37_03450"
     CDS             complement(1048..2040)
                     /locus_tag="PLI37_03450"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOQ58105.1"
                     /translation="MAILAFQKPEKVIMLEADEQFGKFEFRPLEPGFGITIGNSLRRI
                     LLSSLEGYAINSVRIDGVDHEFASIPGVLDDMTNIILNLKQIRFKQIIEDTDFEKVSL
                     TVSGLQELKAGDLGKAFTAFEVLNPDLKICSMEKSVSFNMELTVNKGRGYVPAEEQEL
                     GEVDVNVIAIDSIYTPIKNVKYSVENYRVEQKTDYEKLVLEIATDGSIHPKDALKEAA
                     KILIYHFMLFSEEKITLDVSESDGGEEFDEEVLRMRQLLKTKLVDLDLSVRALNCLKA
                     ANVETLGHLVTFNKTDLLKFRNFGKKSLTELEDLLSKMDLSFAMDISKYKLEKE"
     gene            complement(2053..2658)
                     /gene="rpsD"
                     /locus_tag="PLI37_03455"
     CDS             complement(2053..2658)
                     /gene="rpsD"
                     /locus_tag="PLI37_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOQ58106.1"
                     /translation="MARYIGPKSKIARKFGEPIFGPDKVLSKKNYPPGMHGSNRRRKT
                     SEYGIQLREKQKTKYTYGVLERQFRKTFERAARSKGITGEVLLQLLESRLDNLVYRLG
                     LAPTRDAARQLVVHRHIVVDGKVVNIPSATIKPGQVVGVRERSKALEVIDNALSGFNH
                     SKYPWLEFDAASKSGKYLHVPERADIPENIKEQLIVELYSK"
     gene            complement(2679..3068)
                     /gene="rpsK"
                     /locus_tag="PLI37_03460"
     CDS             complement(2679..3068)
                     /gene="rpsK"
                     /locus_tag="PLI37_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOQ58107.1"
                     /translation="MAKKTVAAKKRVVKVDAVGQAHIHSSFNNIIVSLANGNGQIISW
                     SSAGKMGFRGSKKNTPYAAQMAAQDCAKVAYDLGLRKVKAYVKGPGNGRESAVRTIHG
                     AGIEVTEIIDVTPLPHNGCRPPKKRRV"
     gene            complement(3094..3474)
                     /gene="rpsM"
                     /locus_tag="PLI37_03465"
     CDS             complement(3094..3474)
                     /gene="rpsM"
                     /locus_tag="PLI37_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOQ58108.1"
                     /translation="MAIRIVGVDLPQNKRGEIALTYIFGIGRSSAQKILAEAGVDKDI
                     KVKDWTDDQAGAIREIIGHSYKVEGDLRSEVQLNIKRLMDIGCYRGIRHRIGLPVRGQ
                     STKNNARTRKGKKKTVANKKKAAK"
     gene            complement(3516..3632)
                     /gene="ykgO"
                     /locus_tag="PLI37_03470"
     CDS             complement(3516..3632)
                     /gene="ykgO"
                     /locus_tag="PLI37_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOQ58109.1"
                     /translation="MRVRASVKKRSADCKIVRRKGRLYVINKKNPKFKQRQG"
     gene            complement(3645..3863)
                     /gene="infA"
                     /locus_tag="PLI37_03475"
     CDS             complement(3645..3863)
                     /gene="infA"
                     /locus_tag="PLI37_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOQ58110.1"
                     /translation="MAKQAAIEQDGTIVEALSNAMFRVELENGHEITAHISGKMRMHY
                     IRILPGDKVRVEMSPYDLSKGRISFRYK"
     gene            complement(3880..4665)
                     /gene="map"
                     /locus_tag="PLI37_03480"
     CDS             complement(3880..4665)
                     /gene="map"
                     /locus_tag="PLI37_03480"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOQ58111.1"
                     /translation="MIYLKTEEEIDYLRESSQIVAKALAEVAKHIQPGVTTKYLDQVG
                     EEYIRSCGAVPSFLHYNGYPASLCISVNEEVVHGIPSVQRVLHEGDIVSVDCGAYKNG
                     FHGDSAYTFCVGEVNEELRALMRTTKEALYLGIEQAVDGSRLGNIGHAIQQHCQARGY
                     TVVREMVGHGIGRNLHEGPEVSNVGKRGAGPLLRTGMVIAIEPMINMGRRNIVIENDG
                     WTVRTADRKPSAHFEHTVVVREGKAEILSSFDYIKEVIGERFI"
     gene            complement(4675..6003)
                     /gene="secY"
                     /locus_tag="PLI37_03485"
     CDS             complement(4675..6003)
                     /gene="secY"
                     /locus_tag="PLI37_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOQ58112.1"
                     /translation="MKLIETLKNIWKIEELKSRLLTTLLLVVVFRFGSFVVLPGIDPQ
                     MLTGLHAQTREGLMSLLDMFTGGAFSNASVFGLGIMPYISASIVIQLLSIAVPYFQKL
                     QREGESGRRKMNQYTRYLTVAILLLQGPAFLVNLSANGVPLPSGLWFRMSSVILLASG
                     SMFTLWLGERITDKGIGNGVSMIILIGIIARLPQSLFQEFVSRLSANSGGLVMFLGEL
                     LLLLAVTVFAILLVQGTRKIPVQYAKRIVGNKQYGGARQYIPLKVNAAGVMPIIFAQA
                     IMFIPATVIQFSKAGATSAFAIAFSDITGFWYNFIYAILIILFTWFYTAITINPVQMA
                     EDMKRNGGFIPGVKPGKRTADYLDEIMSRITFPGSLFLAFIAIMPAFAKFFGVSSGFS
                     QFFGGTSLLILVGVVLDTLQQIESHLLMRHYDGLLKSGRIKGRTGSIAAY"
     gene            complement(6019..6516)
                     /gene="rplO"
                     /locus_tag="PLI37_03490"
     CDS             complement(6019..6516)
                     /gene="rplO"
                     /locus_tag="PLI37_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOQ58113.1"
                     /translation="MNLNNLKPAPGSVKTRKRIGRGPGSGLGGTSTRGHKGAKSRSGY
                     SKRRGFEGGQMPLQRRLPKFGFTNINRVEYKAINLDILQTLAESKSYKVINRDVLVQA
                     GFISASQKVKILAKGSLSANLHVVADAFSAAAVKAIEAAGGKAQLVSEFTAPPQKEEN
                     KAIEE"
     gene            complement(6527..6703)
                     /gene="rpmD"
                     /locus_tag="PLI37_03495"
     CDS             complement(6527..6703)
                     /gene="rpmD"
                     /locus_tag="PLI37_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOQ58114.1"
                     /translation="MSVIKIKQVKSRIGAPKDQKRTLDALGLKKLNSVVEHEENAAIL
                     GMVNKVKHLVKIVE"
     gene            complement(6717..7235)
                     /gene="rpsE"
                     /locus_tag="PLI37_03500"
     CDS             complement(6717..7235)
                     /gene="rpsE"
                     /locus_tag="PLI37_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOQ58115.1"
                     /translation="MAGQLNRVKTTNDLELKDKLVAINRVTKVTKGGRAFSFSAVVVV
                     GNEDGIVGYGLGKAGEVTAAISKGAEAAKKNLIKIPVHKGTIPHEQSAKYSGAKVLIK
                     PATPGTGVNAGSAMRAVLESAGVTDVVAKSLGSSNPHNMVKATIKALSELRDAYMVSQ
                     NRGISVERVFKG"
     gene            complement(7240..7584)
                     /gene="rplR"
                     /locus_tag="PLI37_03505"
     CDS             complement(7240..7584)
                     /gene="rplR"
                     /locus_tag="PLI37_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOQ58116.1"
                     /translation="MTTKELRRIKIKQRVRSKSFGTAASPRMTVFRSNKQIYVQFIDD
                     REGKTLASASSLKLETKTGKREQAAKVGEMAAKNAIEAGITTVVFDRNGYLYHGRIKE
                     LADAARKGGLKF"
     gene            complement(7606..8163)
                     /gene="rplF"
                     /locus_tag="PLI37_03510"
     CDS             complement(7606..8163)
                     /gene="rplF"
                     /locus_tag="PLI37_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOQ58117.1"
                     /translation="MSRIGKLPIVIPSKVSVEVDKNNVVTVKGPKGELQQALTGGITA
                     EVKDNHVYVSRPTDGKEHRSLHGLYRALIHNMVVGVSEGFRSVQELVGVGYRASNDGQ
                     LLKLALGYTHEIYLLLPDEVKVETKSERNKNPLIMLESCDKQLLGQICAKIRSFRKPE
                     PYKGKGIRFQGEEVRRKAGKSAVKK"
     gene            complement(8182..8577)
                     /gene="rpsH"
                     /locus_tag="PLI37_03515"
     CDS             complement(8182..8577)
                     /gene="rpsH"
                     /locus_tag="PLI37_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOQ58118.1"
                     /translation="MTDPIADYLTRLRNAISANHRVVEIPASNLKKEITKILLQKGYI
                     LNYKFVEDGPQGSIKIALKYDPLSKQNAIKKLIRVSTPGLRKYVGHREIPRVLNGLGI
                     AVISTSRGVMTDKEAKELKLGGEVLCYVY"
     gene            complement(8638..8907)
                     /gene="rpsN"
                     /locus_tag="PLI37_03520"
     CDS             complement(8638..8907)
                     /gene="rpsN"
                     /locus_tag="PLI37_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOQ58119.1"
                     /translation="MSKESMKAREVNRAKLIAKYAEKRARLKAEGNYEALQDLPKNSC
                     PVRIHNRCKITGRPKGYMRQFGISRIQFREMASQGLIPGVRKASW"
     gene            complement(8922..9473)
                     /gene="rplE"
                     /locus_tag="PLI37_03525"
     CDS             complement(8922..9473)
                     /gene="rplE"
                     /locus_tag="PLI37_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOQ58120.1"
                     /translation="MNTANLKKEYQERIIPALTKEFGYTSVMQVPKLEKIVINQGLGK
                     AVADRKIIETAIAELTAITGQKAVVTTSKKDISNFKLRKKMPIGAMVTLRRDKMYEFL
                     ERLVRVALPRIRDFKGIESKLDGHGNYTLGVQEQIIFPEINIDNITHLAGMNITMVTS
                     AKTDEEGYALLREFGLPFKNINN"
     gene            complement(9473..9793)
                     /gene="rplX"
                     /locus_tag="PLI37_03530"
     CDS             complement(9473..9793)
                     /gene="rplX"
                     /locus_tag="PLI37_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOQ58121.1"
                     /translation="MTKLHIKKGDTVYVNAGEDKGKTGRVLEVDVQKRRAIVEGVNFV
                     SRHTKPNAKSPQGGILKKEAPVHISNLNPLDPKSGKPTRIGRVRNEKGKLVRISKKSG
                     EEIK"
     gene            complement(9816..10181)
                     /gene="rplN"
                     /locus_tag="PLI37_03535"
     CDS             complement(9816..10181)
                     /gene="rplN"
                     /locus_tag="PLI37_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOQ58122.1"
                     /translation="MIQAESRLNVADNSGAKEVLCIRVLGGTGRRYASVGDIIVVAVK
                     NVIPSSEIKKGVVSRAVIVRTKKEIKRADGSYIRFDDNACVMLTNAGELRGSRIFGPV
                     ARELRNTNMKIVSLAPEVL"
     gene            complement(10184..10441)
                     /gene="rpsQ"
                     /locus_tag="PLI37_03540"
     CDS             complement(10184..10441)
                     /gene="rpsQ"
                     /locus_tag="PLI37_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOQ58123.1"
                     /translation="METRNLRKERYGVVIGNKADKSITVVSKFKEKHPIYRKFVSKTK
                     KYYAHDEKNECNIGDTVRIMETRPLSKTKRWRLVEIIERAK"
     gene            complement(10453..10650)
                     /gene="rpmC"
                     /locus_tag="PLI37_03545"
     CDS             complement(10453..10650)
                     /gene="rpmC"
                     /locus_tag="PLI37_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOQ58124.1"
                     /translation="MKAKEVRELTTKEIMERVDAEVDRLNTLTLQHSISPLDNPVQIK
                     ESRRLIARLRTILVERNKENN"
     gene            complement(10656..11087)
                     /gene="rplP"
                     /locus_tag="PLI37_03550"
     CDS             complement(10656..11087)
                     /gene="rplP"
                     /locus_tag="PLI37_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOQ58125.1"
                     /translation="MLQPKRTKFRRQQKGRMKGNAQRGNQLAFGSFGIKTLENKWITG
                     QQIESARVAVTRYMQRQGQIWIRIFPDKPFTKKPAEVRMGKGKGAPEGFVAPVTPGRI
                     LIEVEGVPYDVAKEALRLAAQKLPVTTKFVVRRDYDQNQNV"
     gene            complement(11106..11855)
                     /gene="rpsC"
                     /locus_tag="PLI37_03555"
     CDS             complement(11106..11855)
                     /gene="rpsC"
                     /locus_tag="PLI37_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOQ58126.1"
                     /translation="MGQKVNPISNRLGIIRGWDSNWYGGDSYGDSLLEDSKIRKYLNV
                     RLAKASVSRIVIERTLKLVTITVCTARPGIIIGKGGQEVDKLKEELKKITDKDVQINI
                     FEIKRPEMDAVIVANNIARQIEGKMAYRRAVKMAIASTMRMGAEGIKVQVSGRLNGAE
                     MARSEMYKEGRTPLHTFRADIDYALAEALTKVGLIGIKVWICRGEIYGKVDLAPQFGT
                     TQGSGRGERSFAGERREDRKGGFRKKRNSNR"
     gene            complement(11827..12270)
                     /gene="rplV"
                     /locus_tag="PLI37_03560"
     CDS             complement(11827..12270)
                     /gene="rplV"
                     /locus_tag="PLI37_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOQ58127.1"
                     /translation="MGARKRIAADARKEALKTMYFAKLNGVPTSPRKMRLVADMIRGK
                     EVFLALGILKFSSKEAAGRMEKLLRSAISNWEQKNERKAEAGELYVSEVKVDSATMLK
                     RLRPAPQGRGYRIRKRSNHVSLSINTISKNEQNIKSDGTESKSDK"
     gene            complement(12286..12555)
                     /gene="rpsS"
                     /locus_tag="PLI37_03565"
     CDS             complement(12286..12555)
                     /gene="rpsS"
                     /locus_tag="PLI37_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOQ58128.1"
                     /translation="MSRSLKKGPYINVKLENKVLAMNESGKKSVIKTWARASMISPDF
                     VGHTIAVHNGNKFIPVYITENMVGHKLGEFSPTRTFRGHSGNRKK"
     gene            complement(12577..13401)
                     /gene="rplB"
                     /locus_tag="PLI37_03570"
     CDS             complement(12577..13401)
                     /gene="rplB"
                     /locus_tag="PLI37_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOQ58129.1"
                     /translation="MAVRKLKPTTPGQRHKIIGAFDTITASAPEKSLVFGLTKSGGRN
                     NAGKMTMRYIGGGHKRRLRVIDFKRNKDGVPATVKTIEYDPNRSARIALLYYADGEKR
                     YIIAPNGLEVGMTIMSGDNAAPEIGNALALKNIPVGTVIHNIELRPGQGAKMVRSAGA
                     FAQLVSKEDNYVGIRMPSGEIRRVLDSCKATVGSVGNSDHSLERSGKAGRSRWLGRRP
                     RTRGVAMNPVDHPMGGGEGRASGGHPRSRKGLLAKGLRTRYPKKHSSKYIVERRKK"
     gene            complement(13408..13698)
                     /gene="rplW"
                     /locus_tag="PLI37_03575"
     CDS             complement(13408..13698)
                     /gene="rplW"
                     /locus_tag="PLI37_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOQ58130.1"
                     /translation="MGIIIKPIVTEKMTMITEKLNRYGFRVLPEANKIEIAKAVEKMY
                     NVTVVSVNTSNYRGKNTSRYTRGGVIKGKTAAYKKAIVTLKEGDVIDFYSNL"
     gene            complement(13710..14339)
                     /gene="rplD"
                     /locus_tag="PLI37_03580"
     CDS             complement(13710..14339)
                     /gene="rplD"
                     /locus_tag="PLI37_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOQ58131.1"
                     /translation="MELNVYNIKGEDTGRKVVLSDSIFAVEPNDHVLYLAVKQHLANK
                     RQGTHKTKGRSEITGSGRKLGRQKGGGSARRGDIKSPIMVGGGRAFGPQPRDYSFKLN
                     KKVKSLARKSALTYKMRDNAIVVVEDFQFEKAKTRDFVSIMQNLHLPEKKVVFVLSEA
                     NKNVYLSARNLPKVQVVMFSELDTYKILDAKTLVLMEGSLAGIEQTFNA"
     gene            complement(14339..14956)
                     /gene="rplC"
                     /locus_tag="PLI37_03585"
     CDS             complement(14339..14956)
                     /gene="rplC"
                     /locus_tag="PLI37_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOQ58132.1"
                     /translation="MPGLLGKKIGMTSVFSADGKNIPCTVIEAGPCVVTQVKTVEKDG
                     YRAVQLGFQEKTEKHTNKAEQGHFEKAGVAPQRHLVEFKDFGDEIKCGDVITSELFEA
                     DEFVDVVGTSKGKGFQGVVKRHGFSGVGGASHGQHNRLRAPGSLGASSWPARVFKGMH
                     MAGRTGGERVTIENLQVIKVIPEHHLVLVKGSVPGAKGSIVIIYK"
     gene            complement(15001..15306)
                     /gene="rpsJ"
                     /locus_tag="PLI37_03590"
     CDS             complement(15001..15306)
                     /gene="rpsJ"
                     /locus_tag="PLI37_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOQ58133.1"
                     /translation="MSQKIRIKLKSYDHNLVDKSAEKIVKAVKATGAVVSGPIPLPTH
                     KRIFTVNRSTFVNKKSREQFELCSFKRLIDIYNSSSKTVDSLMKLELPSGVEVEIKV"
     gene            complement(15367..17487)
                     /gene="fusA"
                     /locus_tag="PLI37_03595"
     CDS             complement(15367..17487)
                     /gene="fusA"
                     /locus_tag="PLI37_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOQ58134.1"
                     /translation="MVKADFQYTRNIGIMAHIDAGKTTTSERILFYTGLTHKMGEVHD
                     GAATMDWMEQEQERGITITSAATTTFWNYNNNKYKINLIDTPGHVDFTVEVERSLRVL
                     DGAVAVFCAVGGVEPQSETVWRQADKYNVPRVAYVNKMDRSGADFFSVVSQLRTILKA
                     NPCIIQIPIGAEETFKGVVDLIKMKALLWHDETLGAEYSVEEIPAGLRAEAEEWRDKM
                     LEQVAEYDDRVMQKYFDDPSTIKEEEIHQALRKATLMQALQPVLCGTSFKNKGVQTLL
                     DAVCAYLPSPMDTEAIEGRDPNNPGRVLLRHPDDREPLTALAFKIATDPYVGRLCFVR
                     VYSGVLKSGSYVLNTRNNRKERVSRLFQMHSNKQNPVDVIGAGDIGALVGFKDIRTGD
                     TICDENKPIVLESMDFPDPVIGVAVEPKTQKDLDKLGAGLSKLAEEDPTFTVHTDEQS
                     GQTIISGMGELHLEIIIDRLRREFKVECNQGPPQVSYKEAITREVQLREVYKKQTGGR
                     GKFADIIVKIGPKDADFVGDLQFSSEVKGGNIPTEYIPSIQKGFQTAMRNGVLAGYPV
                     DNLKVTVLDGSFHPVDSDALSFEICAIQAFRNACVKATPVLLEPIMKLEVVTPEENMG
                     DVIADLNRRRGQVGGMESNRSGARIIKAQVPLAETFGYVTALRTITSGRATSSMEFSH
                     YSPMTSIMAKAVLKERAGREDLII"
     gene            complement(17499..17975)
                     /gene="rpsG"
                     /locus_tag="PLI37_03600"
     CDS             complement(17499..17975)
                     /gene="rpsG"
                     /locus_tag="PLI37_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOQ58135.1"
                     /translation="MRKSKPKKRVILPDPVFNDTKVTKFVNHLMYDGKKNISYEIFYS
                     ALKTVKAKLPNEEKSALEIWKTALDNITPQVEVKSRRVGGATFQVPTEIRPDRKESIS
                     MKNIILYARKRGGKSMSDKLAAEIVDAFNNQGGAFKRKEDMHRMAEANRAFAHFRF"
     gene            complement(18117..18503)
                     /gene="rpsL"
                     /locus_tag="PLI37_03605"
     CDS             complement(18117..18503)
                     /gene="rpsL"
                     /locus_tag="PLI37_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOQ58136.1"
                     /translation="MPTIQQLVRKGRVKLTESSKAPALDSCPQRRGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNQKEVNCYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGTL
                     DTAGVDGRKQRRSKYGAKNPKETSKK"
BASE COUNT         4534 a         4492 c         3846 g         5738 t
ORIGIN      
        1 tccttctcat aataaaatta taatcgcttt agaaatgaac aaacttaaca ctttaggaca
       61 aatgcgccac ataaatcaat ctaagatatg caaataaaga taaactactg aagtgtaaaa
      121 gttatgattg aaaaactcaa aaaaaagccg cttcaaaatt gaaacggctt ttggtattga
      181 ctcaaactgt tatggttgga gttaaattat tcctgtttgg actcgtcttc ctgtgctgct
      241 tgttcctcag ttttgccttc tgtaggttca gcgggagctt cggctgtttt ggctttcgaa
      301 tcagcaggag attcgacggc ttcagctttt ggttcaacgg catcggattc agtagctttg
      361 actgccttag cctttggttc tgccaccttg gattcagtag tttcagtaga ttcggcggct
      421 tcttcagctt ctggctcggt agttttggac tcagcggcct caacggtttc agcttctggt
      481 tcggcagtct tagattcaac agcctcagct gctttgccct ttgattctac agcatcagat
      541 tctgcgccct tagcctttgg ttcaacaacc ttggattcga tcttttctgt ttttacttcg
      601 gtggcccctg ctttttcttc actcttcttt tttgaagagc ggcgggtacg cgcttttctg
      661 tccactttgg ttttgagcat gctctcgttg aagtcaacca attcgatgaa acacatctgg
      721 gcattgtcgc ccaggcgata gccggttttg ataatgcgtg tatagccgcc gggtctgtcg
      781 gccactttct gagcaactac ctgaaacaat tcagtgacgg ctctcttatc ttgcaaaaac
      841 ttaaaagcca accttctgga agccgtagta tcttctttgg aaagggttat tataggttct
      901 atttcttttc tcagagcctt ggctttggct gtggttgtga aaatccgttt gtgcataatc
      961 aaagatgttg ccatattaga caacatcgat ttgcgatgcg ctgtcttacg actcaggtga
     1021 tttaatttct tattgtgtct cattatttta ctctttttct aatttatact tggaaatgtc
     1081 catggcaaaa gacaaatcca tctttgacaa caagtcttcc agttctgtca aagatttctt
     1141 gccgaaatta cggaatttca gtaagtcggt tttgttgaaa gtaaccagat gccccaatgt
     1201 ctcgacgttg gctgccttca ggcaattgag cgcacgtacc gacagatcca aatctaccag
     1261 tttagtcttg agcagctgac gcatacgtag tacttcctcg tcaaattctt ctccgccatc
     1321 ggattcactc acgtctaagg tgattttttc ttccgaaaag agcataaaat gatagatcaa
     1381 tattttggcg gcttccttca aagcgtcttt gggatgaatg gaaccatcgg tggcgatttc
     1441 cagcaccagt ttctcgtagt cggttttttg ttcaacacga tagttttcga cggaatattt
     1501 tacgtttttg atgggtgtgt aaatggagtc gatggcaatg acattcacat ccacttcgcc
     1561 caattcctgt tcttctgccg gaacgtaacc gcgtcccttg tttacagtaa gctccatatt
     1621 gaagctgacg cttttttcca tggaacagat tttcaaatcc ggattgagaa cttcgaaagc
     1681 ggtaaatgct ttacccaaat ctccggcttt aagttcctgc aaaccactga ccgtcaagct
     1741 gactttttca aagtcagtat cttctatgat ttgtttaaaa cgtatttgct tgaggttcag
     1801 aataatgttg gtcatatcat ccaaaacacc cggtatactt gcaaattcgt gatccacacc
     1861 gtctatacgc accgaattga tggcataacc ttctagagat gagagcaata tccgcctcag
     1921 ggaattgccg atcgtaatcc cgaaaccggg ctcaagagga cgaaattcga attttccaaa
     1981 ttgttcgtcc gcttccaaca taatcacctt ttcaggtttt tgaaatgcta atatagccat
     2041 aattacggtg ttttatttag agtacaattc aacaatgagt tgttctttga tgttttcggg
     2101 tatatctgct ctttcgggaa catgcagata ttttccggat ttagaagcag catcaaattc
     2161 caaccaggga tatttgctgt ggttaaaacc ggaaagggca ttatcaatta cttccagggc
     2221 tttggagcgt tcgcgcacac caaccacctg gccgggtttt atagtagcgg aaggtatgtt
     2281 taccaccttg ccgtccacaa caatgtggcg gtgtacaacc agctggcggg cagcatcgcg
     2341 agtgggagcc agtcccagac gatacaccag gttatcgagc ctcgattcca gcaactggag
     2401 caacacttcg ccggtaatcc ctttagagcg tgcagccctt tcgaaggtct tgcgaaattg
     2461 tctttccaat acaccgtaag tgtattttgt tttttgtttt tcacgtagct gtatcccgta
     2521 ttctgaggtt ttcctgcgac gattgctgcc gtgcataccc ggagggtaat tttttttgct
     2581 taataccttg tcggggccaa aaatgggttc cccaaatttg cgtgcaattt ttgatttcgg
     2641 accaatatat cttgccattg tttaagtcat taatagattt atactcttct tttcttcgga
     2701 ggacgacaac cattgtgggg caatggtgtt acatcgatta tctctgtaac ttcgatgcct
     2761 gctccgtgaa tggtacgtac ggcagactct cttccgtttc cgggtccttt gacatatgcc
     2821 ttgacctttc tcaagccaag atcatatgcg actttggcac agtcctgagc agccatttgg
     2881 gctgcgtaag gggtattttt cttcgaacct ctgaatccca tcttgccggc tgaagaccag
     2941 gatatgatct gtccgttgcc attggccagg gatacaatga tgttgttgaa agaagaatgg
     3001 atgtgcgctt gtcctacagc gtcaactttg acgactcttt ttttagctgc aactgtcttt
     3061 tttgccattg tttattgatt tgatttaaat ttatcactta gccgctttct tcttgtttgc
     3121 taccgtcttt ttcttgcctt tacgtgtacg ggcattgttt ttggtgctct gtccgcgtac
     3181 gggtagaccg atacgatgac ggattccccg gtaacaacca atgtccatta aacgtttgat
     3241 gttcaattgc acttcggagc gtaaatcacc ttcaacctta taggaatgac cgataatttc
     3301 gcgaatcgca ccggcctggt catcagtcca gtctttgact ttaatgtctt tgtccacacc
     3361 ggcttccgcc agtattttct gagcactact gcgccctatc ccgaaaatat aggtcaacgc
     3421 aatttcaccc cttttgtttt gcggcaagtc tacaccaacg attcttatag ccatacttaa
     3481 tctgttatta tattaaaagt ttgaattaca acctactatc cctgacgttg tttaaactta
     3541 gggttctttt tgtttattac gtataaacgg ccttttctgc gtacaatctt gcaatcggca
     3601 ctacgtttct tgactgatgc tcttactctc atttttataa tattttattt gtatctaaat
     3661 gaaatccttc cttttgataa atcgtagggg gacatttcca ccctgacctt gtctccgggt
     3721 aaaatacgta tgtagtgcat ccgcatcttt cccgaaatat gtgccgtaat ttcgtggccg
     3781 ttttccaatt ctacccggaa cattgcattc gacaatgctt cgactatggt tccgtcctgt
     3841 tctattgcag cttgttttgc cataaatgat attttcaatt taaatgaaac gttctccaat
     3901 aacttctttg atgtaatcaa acgaagacaa tatttcggct ttcccctcac gcacaacaac
     3961 cgtatgctca aaatgagccg acggtttacg gtctgcagta cgaactgtcc agccgtcgtt
     4021 ttcgataact atattacgcc tgcccatatt gatcataggc tcaatggcaa taaccatccc
     4081 tgttcttaag agaggacctg ctcctctttt gcctacgttc gacacctccg gcccttcgtg
     4141 taaattacgc ccgataccat gtcccaccat ttcgcgaaca acggtatagc ctctggcctg
     4201 gcaatgttgt tggatagcgt gaccgatgtt ccccagcctg ctgccgtcaa cagcctgctc
     4261 tatgcccaaa tacagggctt ctttggttgt tctcatcagt gcccgcaatt cctcgtttac
     4321 ttctcccaca caaaaggtat aggctgaatc accgtgaaat ccgtttttgt aggcgccaca
     4381 atctaccgaa actatatctc cttcgtgcaa aactctttga accgagggta ttccgtgcac
     4441 cacctcttcg ttaacggaaa tacaaagcga agcgggataa ccattgtaat ggaggaagga
     4501 agggacggct ccgcaagaac ggatatactc ctctcctacc tgatccagat acttggtagt
     4561 aactccgggt tgaatatgtt ttgcaacttc ggccaacgcc ttggccacta tttgactact
     4621 ttctctaaga tagtctattt cttcttctgt cttcaaataa atcattgccc ggctttaata
     4681 agcagctata cttccggtgc gtcctttgat gcggccggac ttcaacaacc cgtcgtaatg
     4741 cctcatcaag agatgacttt ctatttgctg caaagtatcc agcacaacgc ccaccaaaat
     4801 cagcaaagag gttccgccaa agaattgcga aaagccggaa ctgacgccga aaaatttggc
     4861 aaaagcgggc ataatagcaa taaaggctaa gaacaaagag ccggggaagg taattctgga
     4921 cataatttcg tccagatagt cggctgttct cttgccgggt ttgactccgg gaataaagcc
     4981 accgttacgc ttcatatctt ctgccatttg aaccggatta atagttatgg cagtgtagaa
     5041 ccaggtaaac aatataatga gtatggcgta aataaaatta taccaaaagc ctgtgatatc
     5101 gctgaaagca atggcaaaag cactggtggc accggctttt gagaattgaa tcacagtggc
     5161 gggtataaac ataatggcct gagcaaagat aattggcata actccggcag cattcacttt
     5221 gagtggaata tactgacggg ctcctccgta ttgcttgttg ccgacaattc ttttggcgta
     5281 ttggacggga atttttctgg ttccctgcac caaaagtatc gcaaatacgg taactgccag
     5341 caaaagcagc aattcaccga ggaacatgac caatcccccc gagttagccg atagacggga
     5401 aacaaactcc tgaaacagag attgaggcaa acgggcaata ataccaatca aaatgatcat
     5461 ggaaacaccg ttaccgattc ctttgtcggt gatgcgttcg cctaaccaga gggtaaacat
     5521 actacccgag gccagcagga ttaccgacga catacggaac caaagcccgg aaggaagagg
     5581 tactccgttg gcagagagat tgaccaaaaa agccggacct tgaagcaaaa ggatagctac
     5641 cgtcagataa cgggtgtatt gattcatttt gcggcggccg ctttctcctt cgcgttgcaa
     5701 cttctggaaa tagggaacag ctatactcag caactgtatc acaatggatg cggaaatgta
     5761 aggcatgata cccagaccaa atactgaagc gttcgaaaaa gcgcccccgg taaacatatc
     5821 cagcaaggac atcaaacctt cgcgggtctg agcatgtaaa ccggttaaca tctgaggatc
     5881 gatacccgga agcaccacaa aagagccgaa gcggaaaacc accaccaaca aaagagtggt
     5941 cagtaaacgg cttttgagct cttctatctt ccagatattc tttagggttt ctatcagttt
     6001 cattcgattg tgtgtttatt attcttcaat cgctttattt tcttcttttt gaggaggagc
     6061 tgtaaactca ctgaccaatt gtgccttgcc tccggcggcc tcaatagcct taacagcagc
     6121 agccgaaaaa gcatcggcaa caacatgcaa gttggcactc aaactgcctt tagccagtat
     6181 cttaaccttt tgcgaggcag aaataaaacc ggcttgcact aaaacatctc tgttgattac
     6241 cttataagat ttgctttcgg ccaaagtttg taaaatatcc agattgatgg ctttatactc
     6301 aacacggttg atgttggtaa aaccaaattt gggcaaacgc cgttgtaaag gcatctgtcc
     6361 tccttcaaaa ccacgccttt tggaataacc cgagcgcgat tttgctcctt tatgacctct
     6421 ggtcgaagta ccgcccagac ccgatcccgg accgcgtcca atacgtttac gagtttttac
     6481 cgagcctggt gccggtttga gattatttag attcatgctt gtattattat tcaacgattt
     6541 tcactaagtg ttttacttta ttgaccatgc caaggatagc tgcattctcc tcgtgttcta
     6601 ccacactgtt gagttttttc aatccaagag catccagggt tctcttttga tctttaggtg
     6661 cgccaatacg acttttaact tgtttgatct ttataaccga catgatgttt tcctccttat
     6721 cctttaaata cacgttcaac tgaaataccc ctgttttgcg aaaccatata agcgtcacgc
     6781 aattcactca gagctttaat agtagcttta accatattgt gaggattaga tgagcccagc
     6841 gattttgcta ccacatcggt aacgccggca ctttcgagca cggcacgcat ggcgctaccg
     6901 gcgttgactc cggtccccgg tgtagcgggt ttgataagga ccttggcacc actgtacttg
     6961 gcagactgtt cgtgaggaat agtgcctttg tgtacaggta ttttgatcaa gtttttcttg
     7021 gctgcttcag ctcctttgga aatagcggct gttacttcgc cggccttacc caggccgtag
     7081 cccactatgc cgtcctcgtt gcctactacc acaacggcag aaaagctaaa ggcacgtcca
     7141 cccttggtaa ccttggttac ccgattaatg gctaccaatt tgtctttcaa ctctaaatca
     7201 ttagttgtct taactctatt caattgtcct gccatgatgt taaaatttaa gaccgccttt
     7261 acgagcggca tcagccaatt ctttaattct tccgtgatag agatagccgt tgcggtcaaa
     7321 aaccactgta gtgatacctg cttcaatagc attcttggcg gccatttcac ccacttttgc
     7381 agcctgttcc cttttgccgg tttttgtttc gagcttcaaa gatgaagcag aggccaaggt
     7441 tttcccttct ctatcgtcta taaattgcac gtaaatctgc ttgttgcttc tgaaaacggt
     7501 catacggggg cttgcggcgg taccgaagct cttagaacgt acccgttgct ttattttgat
     7561 tcttcttaat tctttagttg tcatatctgg tagtgtttta tacgattatt tcttgacagc
     7621 agacttaccg gcctttctgc gaacctcctc accctggaag cggatacctt tgcccttgta
     7681 gggttcgggt ttacggaaag agcgtatctt ggcacaaatc tgtccaagca attgtttatc
     7741 gcaggattca agcataatca gcggattctt gttacgttcc gacttggttt ctaccttcac
     7801 ctcatccggc aaaagcaagt aaatttcgtg tgtataacca agagccaatt taagtaattg
     7861 gccatcgtta ctggcacgat aacccacacc taccaactcc tgtacactgc gaaaaccctc
     7921 ggacactcct accaccatgt tgtgaataag agcacgatac aggccatgca gggaacggtg
     7981 ttctttaccg tcggtgggac gggaaacata tacgtgatta tcttttacct ctgcggtgat
     8041 gcctccggtc aaagcctgtt gcaattcacc tttaggtcct tttacagtaa ccacgttatt
     8101 cttgtcaacc tctacgctta ccttggaggg aatgactatc ggcaattttc caattcttga
     8161 catattattc tcctcctgcg attaataaac gtaacataat acttcaccgc caagttttaa
     8221 ctccttggct tccttgtcgg tcatcacacc ccgggaggtt gaaataacag caatgcccaa
     8281 accgttcagt acgcggggaa tctcgcgatg gcctacatat ttgcggagac cgggagtaga
     8341 tacacgaatc agttttttaa tggcattttg tttactcaga gggtcgtatt ttaaggcaat
     8401 tttaatacta ccctggggac catcttctac gaacttatag ttcagaatat aacccttttg
     8461 taaaaggatt ttggtgatct cctttttcaa attagacgcg ggaatttcga ctaccctatg
     8521 attggcactg atagcattcc gtaatcttgt caaataatct gcgataggat ctgtcataat
     8581 gttggtttaa attaatccgg actccccgga caatatttaa ttatataatt tgaatgttta
     8641 ccagctggct ttacgaacac cgggtatcaa accttgtgaa gccatttcgc ggaattgaat
     8701 acgcgagatc ccaaactgac gcatgtaacc tttaggtctg ccggtaattt tgcaacggtt
     8761 gtgtatgcgt accgggcagg aatttttagg taaatcctgt aatgcttcgt agttaccctc
     8821 agctttgagt ctggctcttt tttcggcgta cttggcaata agttttgctc ggtttacctc
     8881 gcgggctttc attgattctt ttgacatact gctattttat cttaattatt tatgtttttg
     8941 aaaggcaagc caaattccct tagcaaggca tagccttctt cgtcggtttt ggcgctggtt
     9001 accatggtga tattcatgcc tgccaagtgg gtaatattgt caatgttgat ttcaggaaag
     9061 ataatctgct cctgaactcc caatgtgtaa ttaccatgtc cgtccaattt gctttcgatt
     9121 cccttgaagt ctctgatacg gggcaaagct acgcgaacaa ggcgttccaa aaattcgtac
     9181 atcttgtcgc gacgcagagt aaccatcgct ccgatgggca ttttcttacg aagcttaaag
     9241 tttgatatgt cttttttgga cgtggtaaca acagctttct gtccggtaat ggcggttaac
     9301 tcggctatgg cagtttcaat aatctttctg tctgctacag ccttacccaa accttggtta
     9361 ataactattt tttctaattt ggggacctgc atcactgagg tgtagccaaa ttcctttgtt
     9421 aaagcaggaa taatgcgttc ttgatattct ttcttgaggt tagctgtatt cattacttaa
     9481 tttcctcccc tgattttttg gatattctga ctaatttccc tttttcgttc ctgacacgac
     9541 caatgcgagt aggcttaccc gatttaggat ccaagggatt taaattggaa atgtgaacgg
     9601 gggcctcttt tttaagtatg cctccctgag ggctttttgc attaggctta gtgtgcctgg
     9661 atacaaaatt gaccccttcg acgatggctc gtcttttttg tacgtccact tcgagcacgc
     9721 gacctgtttt acccttatct tcgcctgcat tcacatatac tgtgtcgcct tttttgatat
     9781 gtaactttgt cattataact ggtatttcta tacaattaaa gtacttcggg agccaaagaa
     9841 actatcttca tattggtatt acgaagctcc ctagcaaccg ggccaaaaat acggcttcca
     9901 cgcaactcgc cggcattggt caacatcaca caagcgttgt cgtcaaaacg gatgtaagaa
     9961 ccgtcggctc tttttatctc ctttttggta cgaactatta cggccctgga tacaacacct
    10021 ttcttaattt cactggaggg aatcacattc tttaccgcta ccacgataat atctcccacc
    10081 gaggcataac gtctcccggt gcctcccagc acgcggatac acaacacctc ttttgcacca
    10141 ctgttatcgg ccacattgag cctgctttct gcttgtatca tgattattta gctctttcga
    10201 tgatttcaac taatctccat ctcttggttt tactcaaggg acgggtttcc ataatcctta
    10261 cagtatcacc gatgttacat tcgttctttt cgtcgtgggc ataatacttt tttgtcttgc
    10321 tgacgaattt tctgtaaatc ggatgcttct ccttaaactt actcacaacg gtgatggatt
    10381 tatctgcttt gttgccgatg acaacaccgt atctctcttt tcttaaattt ctggtttcca
    10441 tcaggctatg tattagttat tttctttatt gcgttcaact aaaatggttc tcagccgtgc
    10501 aatcaaccta cgactttctt ttatctgaac aggattgtcc agaggagaaa tgctgtgctg
    10561 aagcgtcaag gtgttcaaac gatcgacttc tgcgtcgacc cgctccatta tttccttagt
    10621 ggtcaattct ctgacttctt tagctttcat aggtcttata cattttgatt ttgatcataa
    10681 tcacgtctga cgacaaactt agtcgtgaca ggcaactttt gggctgccag acgcaatgct
    10741 tctttggcaa catcgtaagg tacgccttct acctctataa gtattctgcc cggagttacc
    10801 ggggcaacaa aaccctcggg agcacctttt ccttttccca tacggacttc ggcgggcttc
    10861 ttggtaaaag gtttatccgg gaaaatccgg atccatatct gaccctgacg ttgcatataa
    10921 cgggttacag cgacacgggc agattcaatc tgctggccgg taatccactt gttttccaga
    10981 gtctttattc caaaagaacc aaaggccaat tgattgccgc gttgggcatt ccctttcatg
    11041 cggccctttt gttgtcttct gaatttcgtt cttttaggct gtaacattgt ttcgcgatta
    11101 taatattatc tattgctgtt tctcttcttg cggaagccac ctttccggtc ttcacgacgc
    11161 tcgccggcaa aagacctttc gcctctgcca ctaccctgag tagtaccgaa ctggggagcc
    11221 aaatctactt taccgtaaat ttcacctctg cagatccaaa cttttatacc tatcagacca
    11281 actttggtca gagcttctgc cagggcataa tcgatgtcgg ctctgaaagt atgcaggggc
    11341 gtacgtcctt ctttatacat ttcggaacgt gccatttctg ctccgttcaa gcggcctgac
    11401 acctgtactt tgatgccttc ggctcccata cgcatggtgg aggcaatggc catttttacg
    11461 gcacggcgat aagccatttt tccttctatc tgtcgtgcaa tattattagc cacaataaca
    11521 gcgtccattt cggggcgttt gatctcaaag atattgattt gaacatcctt gtcggtgatc
    11581 ttttttaatt cttctttcaa tttatccact tcctgaccac ccttaccgat aatgataccc
    11641 ggacgggcag tacaaacggt aatggtaacc aatttgagtg tacgttcaat tacaatgcgg
    11701 gaaacactcg ctttggccag acggacattc agatacttac gaatcttgct gtcttccaac
    11761 aaagaatctc cataactatc accaccatac cagttagaat cccaacctct gatgatacct
    11821 aaacggttac ttatcggatt tactttctgt cccatctgac tttatgtttt gttcgttttt
    11881 acttatcgtg ttaatactca ggcttacatg attggagcgc ttgcgtatgc gataccctct
    11941 accctgggga gcgggacgca gccttttcaa catagtggca gagtcaactt taacctcgct
    12001 gacatacaac tcacctgctt cggccttgcg ctcgtttttc tgttcccaat tggagatggc
    12061 ggaacgaagc aatttttcca tgcggccggc agcttcctta gacgaaaact tcagtatgcc
    12121 cagggccagg aatacttctt tgccgcgtat catatcggca accagacgca ttttgcgagg
    12181 cgacgtagga acaccgttca atttggcaaa atacatcgtc ttcaaggctt ctttccttgc
    12241 gtctgctgct attctttttc ttgcacccat tgtctctatc tgtttttatt tttttctatt
    12301 tccactgtgg cctctgaacg tacgagtcgg agagaattct cccaacttgt gtccgaccat
    12361 gttttcggtg atatacaccg gtatgaactt atttccattg tgaaccgcta tggtatgtcc
    12421 cacaaaatcc ggagaaatca tagaggctct ggcccaggtc ttgatcaccg attttttgcc
    12481 ggattcattc atggctaaaa ctttgttttc aagttttaca ttgatatacg gtcccttttt
    12541 taatgaacga ctcataatat ttcctacttt atcctattat tttttccttc tttctacaat
    12601 gtacttgctt gaatgtttct taggatatct tgttctaaga cccttagcca acaaaccttt
    12661 gcgcgatctt ggatgcccac cggaagcgcg gccttcgcca ccacccatgg ggtgatcaac
    12721 cgggttcata gctacaccac gggttctggg acgacgaccc agccacctcg aacggccggc
    12781 tttaccggag cgctccaggc tgtggtctga attgccgaca ctgccaactg tggctttgca
    12841 agaatctaac accctgcgga tttctcccga tggcatacga atacctacgt aattgtcttc
    12901 tttggaaacc aattgcgcaa aagcaccggc ggaacgtacc atcttagcac cttgtccggg
    12961 acgaagctcg atattgtgaa taacggtccc cacaggaata tttttcagtg ccagggcatt
    13021 tccgatttcc ggagctgcat tatctcccga cataatggtc atcccgactt caagtccgtt
    13081 gggagctata atgtatcttt tctcaccgtc ggcataataa agcaatgcta tgcgtgctga
    13141 acggttggga tcgtactcaa tggttttcac tgttgcagga acgccatctt tatttctctt
    13201 gaaatcgata acacggagtc ttcttttatg accgcctccg atatagcgca tagtcatttt
    13261 ccccgcgtta ttgcgtccgc ccgatttagt cagaccgaat acaagagatt tttccggtgc
    13321 actagcagtt atggtatcga acgcaccgat aatcttatgt ctttgcccgg gtgttgtggg
    13381 ctttaatttt cgtactgcca tcttatttta taaattgctg taaaaatcaa tcacatcacc
    13441 ttctttcaag gtaactatgg cttttttgta ggccgccgtt ttgcccttta ttactccgcc
    13501 tctggtatag cgactggtgt ttttgcctct gtagttgctg gtgttaacac taaccacagt
    13561 aacattgtac atcttttcga ccgccttggc aatttcaatt ttattcgctt ccgggagtac
    13621 acggaatccg taacgattta acttttccgt aatcatcgtc atcttttcgg taacgatagg
    13681 cttaattata attcccattg tttttctcct tatgcgttaa aagtttgttc aattccggcc
    13741 aacgaacctt ccatcagcac cagtgttttt gcgtcaagta ttttataagt atctaattca
    13801 gaaaacatta caacttgcac tttcggcaaa ttacgagccg acaaatatac gtttttattt
    13861 gcttccgaca atacaaaaac tacctttttt tcaggaagat gcaaattttg catgattgat
    13921 acaaaatctc tggtcttggc tttctcaaac tggaaatctt cgaccaccac aatggcgttg
    13981 tcgcgcatct tgtaggtcaa ggccgatttt cgagccagcg acttcacttt tttgttcaat
    14041 ttaaaactgt aatcacgggg ctgagggcca aatgctctgc ctccaccaac cataatcggc
    14101 gatttgatat ctccgcggcg tgcactgccg ccacccttct gacgaccaag ttttctgccg
    14161 gaacctgtaa tctcactacg tcccttggtt ttgtgcgttc cttgcctttt gttggccaaa
    14221 tgttgtttaa ccgccagata caatacatga tcgttgggtt caacagcaaa aatggaatcg
    14281 ctcaacacaa cctttcttcc ggtgtcttca ccttttatat tatatacatt cagttccatc
    14341 atttatagat tattacaatt gaacctttag ctcccggaac agaaccttta accaaaacaa
    14401 gatggtgttc gggaataact ttgatcacct gaaggttttc gatggtcacc ctttctccgc
    14461 cggtacgtcc ggccatatgc atacccttga aaacgcgggc aggccatgag gatgctccca
    14521 acgaacccgg ggcacgtaaa cggttgtgct ggccgtggct ggctccaccc acacccgaga
    14581 atccatgacg cttgacaaca ccctgaaatc ctttaccttt ggatgttccg acaacgtcaa
    14641 caaattcatc ggcttcaaac aactcagagg tgatcacgtc accacactta atttcgtcac
    14701 cgaaatcttt aaattccacc agatgtctct gaggtgctac cccggctttt tcaaaatggc
    14761 cttgctctgc cttgttggta tgtttttcgg ttttttcctg aaaacccaac tgtacggccc
    14821 gataaccatc tttttctacg gttttaacct gagtaacaac acaaggaccg gcttcaatca
    14881 cagtgcatgg gatgtttttc ccatcggcac tgaaaacgga tgtcattccg attttttttc
    14941 ctaataatcc tggcatttct ctaaattata taattatata aatcatgcat ttttgcgcta
    15001 tcagactttg atttccacct caacgccgct gggtaattcg agtttcatca aagaatcaac
    15061 tgtttttgaa cttgaattgt agatgtcgat cagtctcttg aaagaacaca attcaaactg
    15121 ttcgcgagat tttttgttta cgaaagtaga gcggttcacg gtaaatatcc ttttgtgcgt
    15181 gggcaaagga atggggccgc ttaccacagc tcccgtcgcc ttaacggcct ttacaatctt
    15241 ttcggcggat ttgtccacca ggttgtgatc gtaagatttg agtttaattc tgattttctg
    15301 gctcataaat gttttattaa tggtttattt cggcgatccg tagagctatt ttcactgatc
    15361 tgccgtttat atgattaaat cttccctccc cgcacgttct ttgagtacgg ctttggccat
    15421 aattgaagtc atgggagagt aatgtgaaaa ttccatactg ctggttgccc ggcccgacgt
    15481 aatggtacgc agggcagtta cgtaaccgaa agtctcggcc agcggcacct gtgctttaat
    15541 gattctggct cccgaacggt tagattccat gccgcccacc tggccgcgac gtctgttgag
    15601 gtcggcgatt acatcgccca tattctcttc gggtgtaacc acctcgagtt tcataatggg
    15661 ctccaataac acgggagtag ccttaacaca ggcatttctg aaggcctgaa tggcacaaat
    15721 ctcaaacgag agtgcatcgg agtctaccgg gtggaaggag ccatccaata cggtaacctt
    15781 gagattatct accggataac cggccaaaac cccgttacgc atagctgtct ggaaaccctt
    15841 ctgaatggaa ggtatgtatt cggtgggtat gtttccaccc ttgacttcgc tcgagaattg
    15901 cagatcgcca acaaaatcgg catctttggg gccaatttta acaatgatgt cggcaaattt
    15961 cccccgtccg ccggtttgtt ttttgtaaac ttcacgcagc tgcacctccc gggtaatagc
    16021 ttccttatac gaaacctgag gaggtccttg attgcattcc actttgaatt cgcgacgtaa
    16081 acggtcgata atgatttcca gatgcaattc gcccatgcca ctaatgatgg tttgccccga
    16141 ctgttcatcg gtgtgaacgg taaaagtggg gtcttcttct gctaattttg acagaccggc
    16201 acccagctta tccaggtctt tttgagtttt gggctcaacg gctacgccga ttacgggatc
    16261 ggggaaatcc atcgattcca gtacgatggg tttattttcg tcgcaaatgg tatcgccggt
    16321 gcgtatatcc ttgaaaccta ccaaagctcc gatatctccg gctccgataa catcgacagg
    16381 attctgttta ttagagtgca tctggaacaa cctcgacacg cgttccttac gattgttccg
    16441 ggtattgagc acataactgc ccgacttgag cacaccggaa tagacacgta caaaacacaa
    16501 acggccaacg tagggatcgg tggcaatttt gaacgccaag gcggtgagag gctcccggtc
    16561 gtcgggatgt ctcaacaaaa ctctaccggg attgttggga tccctccctt cgatggcctc
    16621 ggtatccatg ggactgggca aataagcaca gacagcatcc aacagtgttt gcaccccttt
    16681 gtttttgaaa gaagtgccgc agagtacggg ctgcaaggct tgcatcaggg tggccttgcg
    16741 taaagcctga tgaatttcct cttctttgat ggtggaggga tcgtcaaaat atttctgcat
    16801 cacgcgatcg tcgtactctg ctacttgctc cagcattttg tccctccatt cttcggcttc
    16861 ggccctgaga ccggcgggaa tttcttcgac ggaatattcg gctcccaaag tttcatcgtg
    16921 ccacagcaag gccttcattt tgatcaggtc gacgactccc ttgaatgttt cttctgcccc
    16981 aattggaatt tgaataatgc aaggattggc cttgagtatc gttcgcaatt gagaaaccac
    17041 cgaaaagaaa tcggcacccg aacgatccat cttgttgacg taggctacgc ggggcacatt
    17101 atatttatcg gcctgtctcc agactgtctc cgattggggt tctaccccgc cgaccgcaca
    17161 aaaaacagcc acagcaccgt ccagtactct aagagagcgt tctacctcaa cggtgaagtc
    17221 tacgtgcccg ggggtatcaa tcaggttgat cttatattta ttgttgttgt agttccaaaa
    17281 agtggtagta gcagcagaag taatggtaat accccgttcc tgctcctgct ccatccagtc
    17341 catagtggca gcaccatcgt gcacctcacc cattttgtga gtaagcccgg tgtagaaaag
    17401 aatacgctcc gaagtggtag tctttcccgc atcgatatgg gccattatgc ctatatttct
    17461 ggtgtactgg aagtctgctt ttaccatcgt ctgatcaatt aaaacctgaa gtgagcaaaa
    17521 gcacggttgg cttcggccat tctgtgcata tcttccttac gcttgaatgc tccgccctga
    17581 ttgttgaaag catcaacaat ttctgcagcc agcttatcgg acatggattt tcctccacgt
    17641 ttgcgggcat acaaaataat atttttcata gaaatggatt ccttgcgatc cgggcggatc
    17701 tctgtaggaa cctggaaagt ggcacctccc acgcggcggg attttacctc gacttgagga
    17761 gtaatgttgt cgagggcggt cttccagatt tcgagtgcgg atttttcttc gttaggcaat
    17821 ttagccttta cggttttcaa agcggaatag aaaatttcgt acgaaatatt cttcttcccg
    17881 tcatacatca ggtgattgac aaattttgtc accttggtgt cattaaaaac aggatccggg
    17941 aggataaccc gttttttcgg tttgcttttt ctcattttct caaataattg accgttttat
    18001 tgttttggct gttcgtcttc aacgctccca ccggaacatt tactcaaccc ggaaaaacta
    18061 gtctccctta cttaccaata cgctaaaaac tggtgatgaa tgtatgatgc tttaatttat
    18121 tttttgcttg tttccttagg atttttggct ccgtacttgg aacggcgttg tttacgaccg
    18181 tctacaccgg cagtgtcgag tgtgccgcga acaatgtgat aacgaacacc gggtaaatcc
    18241 ttcacacgac ccccgcgaac caacactata gagtgctcct gcaggttatg gccttcgccg
    18301 ggtatatagc agtttacctc tttctggttg gtcaggcgaa ccctggccac tttgcgcatt
    18361 gcagaattcg gttttttggg tgtggttgta tagacacgta cacaaacgcc acgcctttgc
    18421 ggacaggaat ccagagcagg cgctttgctg ctttcggtca gttttaccct tcctttcctt
    18481 actaattgtt gaattgttgg catactactt cttttggtct taaatcatta atccttaaat
    18541 atgtgcccat tttttactca aaaacgggct gcaaagatag aaagtctttt tattttatac
    18601 aaataagttg
//
LOCUS       DAOSTD010000032        18008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_3253_length_18008_cov_16.836183, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000032 DAOSTD010000000
VERSION     DAOSTD010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18008
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3253_length_18008_cov_16.836183"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PLI37_03610"
     CDS             complement(<1..706)
                     /locus_tag="PLI37_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ58137.1"
                     /translation="MTTASDCIRKYRAFLKLEKSLSENTLAAYMSDLQKLMDFSGDKA
                     LTELKHEDLEEFLCHLRDLGIGARSQARILSGIKSFYGFLLIEDYISTDPTELIETPQ
                     LGKKLPEVLSVDEIDKLLASIDLSRKEGQRNKAMLETLYSCGLRVSELTGLKLSCCYF
                     KEEYLLVEGKGSKQRLVPISRKAMKEISLWLIDRNLLPKQKNQEDFVFLNRRGHKLSR
                     SMVFKIIKEQALLAGIN"
     gene            806..1231
                     /locus_tag="PLI37_03615"
     CDS             806..1231
                     /locus_tag="PLI37_03615"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOQ58138.1"
                     /translation="MKIAIINGPNLNLLGKREPDIYGSQNFESYLEILRNDYPQVEFE
                     YFQSNHEGAIIDKLHEIGFSYDGIVLNAAGYTHTSIAIGDAIRAIQTPVIEVHISDIY
                     KREEYRHISRTEAACIASFVGYGMESYRMAVEALLEYIS"
     gene            1243..1875
                     /locus_tag="PLI37_03620"
     CDS             1243..1875
                     /locus_tag="PLI37_03620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOQ58139.1"
                     /translation="MDELLERYICSRIDPEPELLKELRRETYVRFLNPRMSSGHLQGR
                     VLAMLCKMIRPRRVLELGTFTGYSTLCLAEGLPEGSRILSIDRNDELEEHLKTWFSRS
                     ELGSKIDLLFGEALDLIPKLTESFDLVYMDIDKRDYKSCYDLLLQHQPSGTFILIDNT
                     LWNGKPMREISCKDTQSLAIADFNDYIAADSRVEKIILPIRDGLTVLLVK"
     gene            1896..2231
                     /gene="rbfA"
                     /locus_tag="PLI37_03625"
     CDS             1896..2231
                     /gene="rbfA"
                     /locus_tag="PLI37_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOQ58140.1"
                     /translation="METIRQNKISRLIQKELSEVFREQTTALPGTMVTVSMVRVSPDL
                     SVAKAYLSLFPPEKSKEMLANIQNSAKSIRYKMAQRLRFQLRRMPELSFFLDDSLDYA
                     QNIEDLLKK"
     gene            2228..3445
                     /locus_tag="PLI37_03630"
     CDS             2228..3445
                     /locus_tag="PLI37_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOQ58141.1"
                     /translation="MNLAFHIARRYLFSKKSHNAINIISMVSVFGVAAATIAMVCTLS
                     VFNGFQDLLTGLYKNFEPELKIRAFEGKTFRLNSDFDLIRAHKEVAVFCESLEENALI
                     RYKGSQASALVKGVSDNFSELANMDSLVYAGKFVLEEEGFQFASIGIGLASLLGTGSS
                     FIDPVTLYAPKRTGSLNPLNPASAFKTRRILLTATFGIDQPEYDDRIIIVPLNFAREL
                     FEYTDELSSVELKLKKGANLQRVKEDFRQLLGPDFIVEDLKEQKEDIYRINRVEKWIT
                     YLILCFILLIALFNVIGSLSMLILEKKQDAGILRRLGADHKTIRKVFLYEGVMIAFLG
                     AFSGILLGVLLCLIQQHFGLLKLGSGNFIVGSYPVLVKASDLFFILLTVLAVSLPSVW
                     WPVHYYFKNDELP"
     gene            3429..5705
                     /locus_tag="PLI37_03635"
     CDS             3429..5705
                     /locus_tag="PLI37_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOQ58142.1"
                     /translation="MTNFLKIAYGRTLVITGLISWLFVLDFSPLQAQRSYGGRPLSIE
                     EEVAYVRALADYLVELPSFDVDSLLREDSIYHNPNGALRFAHKMFVNLSPENSGITFN
                     LEDGTKIWKLGLRSKGAYSLNVIFEEYIVPPGARLYLYNPDKTQVLGSFTSDNMQESG
                     EFAIAPISGEEIMIEYHEPADAAFPGRLRLTEVNHDYRGPMLLGTKFDKLNLPCVPEL
                     SCQAEYDSIGRSICLLILNGTTYCTGTFVNNTACDGRPLLLTAAHCLENNPSLAGRTV
                     VFMNYLSPRCMHQIRGSEEFSLSGCQALAFSEETDYLLMELKEKIPPDYRLYLAGWSR
                     DTSARYESPFACLHHPYGEGKRYAQTTDSLTYATWPYSGDGFDPDSHWSVRQWQEGHT
                     WTGSSGAPLLNKHHQLIGFLTGGDSGGSSGCGPYQLGDFFVRFQKAWDYESDSLNQLA
                     HWLDPLSSDALTISGLDPNQLNPARRITNLRPTDSIAGLRHENPENGFLFGHNSFEYK
                     IFAEEFRQDSNSMLKGVYLMPSKGVKSSSYPVYIDVMKGGFVPEETLASIELNPYYLE
                     YRNRNFHETSKVHFANAENYVCFDPPLSVGKHFYVAIRLVYPSKTVAAIDSFALYSAY
                     HKERAAKNTVWLNNGTGWEFFTAMNRRAHSSSLWLEPVVMQDTLPVVEPPLVVDTLSQ
                     RIPLYYSFQDRLLHISFPTGWRSPFEIRIFDPAGRLMHFTSTEQETMTLYIPESFKGF
                     YIIQLTERRRRYVQKIVF"
     gene            5860..7551
                     /locus_tag="PLI37_03640"
     CDS             5860..7551
                     /locus_tag="PLI37_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58143.1"
                     /translation="MNKTFKLSILVSLLLILSGCGSKLALDREHFTVTPQVLEAVGGK
                     VDATIDGVFPAKMFPAKGIVTITPVLKYEGGEVEGAPMSFQGEKAVGNNKVIPTAAGG
                     NFSMKSSFDYKPEMKRSELFVRINSVTGNKTQDHPEIKLADGVVSTENLLNAQKAKPS
                     PGADKFQRIISEVTEAQILFLIQQSNVRTSELRSDEIKALVSAISNATQTENKEISGI
                     NVASYASPDGPVKLNESLAGSREKNTVNYLNRELRKAKAQGDINAEFTAEDWDGFKKL
                     LEASDVQDKALILRVLSMYSDPEQREAEIKKLSSAYKVLADEILPKLRRSKIALTVDV
                     IGKSDEEIKALFGSNPSELNVEEILYAATLYSTPAEKEKVYQKTTQLYPNDSRAFNNI
                     GMIKFEAGDVAGAKTWFEKAYTIDKSKESSLNLALCAISEGADNATVEAYLNQAAGAE
                     NYEGALGLLYIKKGDYANAVKAFGDQKSNNAALAQILAKDYSKAKATLAGVEKPDAET
                     YYLQAIVGARTNNKAEIVDGLKKSIAQDPAMAKKAANDIEFAKYLVDAEVASLLS"
     gene            complement(7666..9522)
                     /gene="ispG"
                     /locus_tag="PLI37_03645"
     CDS             complement(7666..9522)
                     /gene="ispG"
                     /locus_tag="PLI37_03645"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOQ58144.1"
                     /translation="MTYCRNLFNYQRRKSSPVNIAQVPLGGSYPVRIQSMTNCSTLDT
                     VCCVEQIKKIVAAGADYVRLTAQGTKEAENLGNIRREIRKQGVEVPLIADIHFNPKAA
                     EMAASQVEKVRINPGNFADPLHIELPKGGVSDLGGVSILELEKQKVRERLLPLLAICK
                     KRGTALRIGVNHGSLSSRMMLRYGDSPRGMVESCMEYLRICVEEDFSRVVISIKSSNT
                     LAMIHTVRLLVKTMEAEGMSFPLHLGVTEAGDGEDGRIKSAVGIGSLLLDGIGDTVRV
                     SLSEDPEVEIPVARKLVEYVEERKGHALINAKAFEGFDPYSYKRRESKKAGIIGKEQV
                     PVVIQDHSQGLTTDYSFADPMPDFIYLGTKTPEAPRKDNIPQICDYRVYREQTQCYPL
                     FEVAQIEELSGCKAALRFLRVCHTDLNEGLLQQLEKEQNLVLWLYSSHLNAAAEMRAA
                     FHTLLNLGSYLPVVVASAYSTEDVENLQVQAATDLGFLLADGFGDGVCISAGGLQAEC
                     LSLMFGILQATRSRISKTEYISCPGCGRTLFNLQSTLAKVKAGTSHLKGLKIAVMGCI
                     VNGPGEMADADYGYVGAGRGRISLYKGKLCVEKNIPEEEALERLLNLIQKDR"
     gene            complement(9497..10024)
                     /gene="purE"
                     /locus_tag="PLI37_03650"
     CDS             complement(9497..10024)
                     /gene="purE"
                     /locus_tag="PLI37_03650"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOQ58145.1"
                     /translation="MKAIVSIIMGSTSDLPVMEKAADLLNEFQIPFEMHALSAHRTPE
                     AVEKFSRTAAERGIKVIIAAAGMAAHLPGVIAASTTLPVIGVPIKASLEGMDALLAIV
                     QMPPGIPVATVGINGATNAALLAVEILAVADQELQARLIAYKTGLKNKIVKADRELQQ
                     LKYEYKTNDLLSESL"
     gene            complement(10029..10406)
                     /gene="gcvH"
                     /locus_tag="PLI37_03655"
     CDS             complement(10029..10406)
                     /gene="gcvH"
                     /locus_tag="PLI37_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOQ58146.1"
                     /translation="MNDPKTLKFTKNHEWIRVEGQEAYVGISDFAQSELGEIVYVDIT
                     TVGESLQKEEVFGSVEAVKAVSDLFIPVGGTVLEANAELEDHPELINKEPYDAGWIIK
                     ISLNNSAELQELLSYEQYKEMIG"
     gene            complement(10440..11873)
                     /gene="rpoN"
                     /locus_tag="PLI37_03660"
     CDS             complement(10440..11873)
                     /gene="rpoN"
                     /locus_tag="PLI37_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOQ58147.1"
                     /translation="MLHQELSLKLQQKLSPQQILRIKLLEIPELEMEERILRELDENP
                     ALELGGDPESKEESLEEEAEEQQDELDLEDYLQDDDDLYGNREYDKNTPDRRETFFGS
                     SALSLRDFLMQQLDMQNLTENEKIIGEYLIGNIDDDGYLRIPLETVSDQISFQAFKEV
                     SLDEMHKMLKVIQDFEPAGVAAQNLKDCLLLQLYRKPKTVLTEQAIHLLEQYFEEFSH
                     KHHDRILHRWGITREEYDAIVGEILKLNPKPGNAWEGMPGESGKQIIPDFIIENIDGN
                     LVLSLNEGNLPEIHVSSRYLRLLQDYNASPHKQSEETRNAAVFARQKVEAAYWFIEAI
                     KQRQQTLLSTMQAIMDYQKEFFMSGNEKKIRPMILKDIANITSYDISTISRVSSSKYV
                     QTSFGIFPLKYFFSESLLTDSGEEVSTIRVKNVLQEIIDNEDKSKPFTDDELEKIMQD
                     KGYRVARRTISKYRSQLKIPVARLRVQ"
     gene            complement(11940..13604)
                     /locus_tag="PLI37_03665"
     CDS             complement(11940..13604)
                     /locus_tag="PLI37_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOQ58148.1"
                     /translation="MSKAVRIFCVNNNKNMLMPMGVSLLEVYQQLSQELNMEPIAARV
                     NNKTESLSYELYNPKRIEFIEMNSASGMRVYVRTLTFLISSAIYELYPEGSFSIEHSV
                     SNGYYCELDLKRECTRKDLDAIKKRVLELIEAGNPIDSVKEETSIVIELFRKRGRKDI
                     VDLLSTFGSLYTTYCKMGEHIDYYYGALAYSSGINKNFDLIPYLDNYLLRIPSRSNPS
                     ELAPLVPQPKMQDVFREFVRWNKIMDLRYVGDLNLMCRQGRATELIKVAEALQEKKIA
                     QLADTIAKGLDKIRIVLISGPSSSGKTTFSKRLSVQLLAMGIRPIAISMDDYFLEREQ
                     TPKDEKGEFDYESLYALDLDLFNRDLGNILEGQEVEIPSFNFALGTKEYKGNKVRLTP
                     QNILILEGIHALNPELLKDVPPEAQFKLYISALTAISLDKHNRIPTTDNRLIRRIIRD
                     SRYRGTSARETIARWDSVRAGEEKWIFPFQENADAMFNSALIFEFSVLKNYIEPVLRE
                     VPQNCVEYSEARRLLRFLDYFVPITDWEIPPTSLLREFVGGSSFRY"
     gene            13720..15540
                     /locus_tag="PLI37_03670"
     CDS             13720..15540
                     /locus_tag="PLI37_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOQ58149.1"
                     /translation="MDYSFYDFLMLVGSLGLFLFGMKMMSEGLQKAASKKLKEFLSAM
                     TSHRYAGVLMGMLVTAIIQSSSATTVMVVSFVNAGLISLVQSISVIMGANIGTTITAW
                     VISVFGFNFNIAELSIPLIGIGIIPLLFSKKNNHKNIGEFIIGFALLFMGLSYLKDSV
                     PDLQNNPEALEFISKFTQKGYGSVLIFLLFGTLLTIVIQSSSATMAVTLIMCAKGWIS
                     FEMGAAMVLGENIGTTVTANLAAISTNVSGKRAALAHTTFNVIGVIWMLIVFFPFVGM
                     IQKIIVNVTGVDPKQLDVFTASLDPGVLKLISDPGITLDDPQLISLRSQLVSLQASTS
                     YALSLFHTMFNLINTGLLIWFTGTIAKIVTKIIPSKKTDEEFQLRYISTNLLSASELA
                     IPEAKKEIDDYITRTRRMFDMVRDLYYEKQESNFEKIYARILKYESISDRVELEIATF
                     LNNVSEGRLSDKSKHQIRQMIRMTSEIESIADSCHNIANTIKRRWDAKVVLTDEMTEH
                     VEIMSGLVYKQLEGMANLILKNSHDTAELLKIRDAEIEINKYRNQLYQENDLDLEAKK
                     YTYQEALYYIDIIRECERLGDYIVNVVEAYTGGSKLFAHI"
     gene            15646..16356
                     /locus_tag="PLI37_03675"
     CDS             15646..16356
                     /locus_tag="PLI37_03675"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOQ58150.1"
                     /translation="MKKNVMLAVVFAGTMCLSLCFTSCDCKCGSKAMKTNVDSLSYAY
                     GVGLGDNLAQNLSSFPAELNMDLFLEAFEKTIKGKTEGLSIQPDQAYQVFQSCLTAIQ
                     MESAQKNKEEAGKFLEENAKREGVVTTESGLQYEVLLAAEGPKPTATDRVSVHYHGTL
                     LDGSVFDSSIDRGEPAQFSLNGVIPGWTEGIQLMSVGSKYKFYLPSDLAYGDNGAGDV
                     IQPGAMLIFEVELLEILK"
     gene            complement(16475..>18008)
                     /gene="ribF"
                     /locus_tag="PLI37_03680"
     CDS             complement(16475..>18008)
                     /gene="ribF"
                     /locus_tag="PLI37_03680"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOQ58151.1"
                     /translation="AMELKGIKNILFDFGGVLVKLNPQACLDAFGALGAPQVADYLTP
                     YGHTGPFGLFENGKIGLEEFRKEIRRIFGIKAGDEEIDRAWAAFLEDIPLENTTIVHN
                     LSKKYRLFLLSNTNPIHIRKLEQFEQAGRPLNQCFEKCYLSYEIGFSKPHKEFFEHVL
                     KDSCLKAEECLLIDDGPANCRSAEALGMKTLCPGSVEDLSKFFPEMLNTVTAEKTVAT
                     IGFFDGVHLGHKQLIASLRREAAQRNLASLVLAFWPHPRMVLHSDYCPQLLNDCSEKL
                     ALLRACGADHTQLVRFDKQLARMSAFEFMNQVLKKEWNVACLLAGYDHRFGKNKEEDF
                     DDYRRYGDVLGIEVLKAGPVLLPDSSKAISSSYIREALLKGEILKANAALGYDYTIKG
                     RVGGGYGVGRKLGFPTANIIPSDPHKLIPLPASYIVLAEVENRLYPGMLNIGQRPTLQ
                     NGCQRSIEVHLIDFSGDLYDKGITVHFKKHLRPERRFPSTEELSDQLKLDREAVLRFF
                     KQ"
BASE COUNT         4743 a         4073 c         4129 g         5063 t
ORIGIN      
        1 tgttgatacc ggccaacagg gcttgctctt tgattatttt aaaaaccatg gaacgtgaaa
       61 gtttatgtcc gcgacggttc aaaaatacaa aatcttcctg atttttttgt ttaggcagca
      121 gatttctgtc gattaaccaa agcgagattt ccttcatcgc tttgcgggaa atgggcacta
      181 atcgctgttt gctccctttg ccttcgacca gtaaatattc ctctttaaag taacagcacg
      241 agagtttcag tccggttagc tccgaaaccc ggaggccgca gctgtagagt gtttccagca
      301 tggctttgtt gcgttggcct tctttacgcg acaagtcgat actggccaaa agtttgtcga
      361 tttcgtctac ggagagcacc tcgggcagtt ttttaccgag ctggggcgtc tcgatgagtt
      421 cggtgggatc ggtcgaaata taatcttcga tcagcaaaaa gccgtaaaaa gatttgattc
      481 cggaaagtat tctggcttgt gagcgcgcac cgataccaag atctctcaga tgacacagaa
      541 attcttccaa gtcctcgtgc ttaagttctg tcagggcttt gtcgccgcta aaatccataa
      601 gcttttgcaa atcgctcata taggcagcaa gggtgttttc tgacagagat ttctccagtt
      661 tgagaaaagc ccggtatttt cttatgcaat cggaagctgt cgtcatattt tttttacctt
      721 tgtcaggcag aaaatgaacc gcacatacgg caagcggggc aaaagtagta aataaatcat
      781 agaggaacag ctaaatttta aatgtatgaa aattgccatc atcaatgggc ctaatctgaa
      841 tctccttgga aaaagagaac cggatatata cggctcacaa aatttcgaat cgtatttgga
      901 aattttgaga aatgactatc ctcaggtgga attcgagtac tttcaatcca atcacgaggg
      961 ggccatcatc gacaaacttc atgaaatcgg gttcagttac gacggcatcg ttttgaatgc
     1021 tgcgggctac actcacacat ccattgcgat tggtgacgcc ataagagcaa tacaaacacc
     1081 ggtaattgaa gttcatattt cggacattta caaaagagaa gaatatcgtc atatttccag
     1141 aacggaagct gcgtgcattg ccagctttgt aggctatgga atggaatcgt accgcatggc
     1201 tgtggaggca ttgttagaat atatttcata attgtgtggt gagtggatga gcttttagaa
     1261 aggtacattt gtagccgtat tgatcctgaa cctgaattgt tgaaggaact cagaagagag
     1321 acatatgtac gttttttaaa tccaaggatg tcgtcaggtc atttgcaggg gcgggtgctg
     1381 gccatgttgt gcaaaatgat ccgccctcga agggttctgg agctgggaac ttttaccggc
     1441 tattccacgt tatgcttagc cgaggggtta cccgaaggtt cacgaattct gagcatagac
     1501 cgcaacgatg aactggaaga gcacctgaaa acttggtttt caagatcgga actgggctca
     1561 aagatcgatc ttctttttgg agaagcgctt gatttaattc ctaaactgac cgaatctttc
     1621 gatttggtgt atatggatat tgacaaaaga gactacaaaa gttgttatga cttattgctt
     1681 caacaccagc caagcggcac ttttattttg attgataaca ctttatggaa cggaaagccc
     1741 atgcgggaaa tttcctgcaa agatactcaa agccttgcca ttgcagattt taacgattat
     1801 attgcagcgg attcccgggt agaaaaaatc atactaccca tccgagacgg gttgaccgtt
     1861 ctgcttgtaa aataaaaaca cataaaaaca gagagatgga aactatcaga caaaataaaa
     1921 taagccggct tatacaaaaa gaattgagcg aagtgttccg ggaacagact acagcgcttc
     1981 cgggtaccat ggtcacagtc agcatggtcc gtgtcagtcc tgatctgagt gtggccaagg
     2041 cttacctgag tcttttccca cctgaaaaaa gcaaagaaat gctcgccaac atacagaaca
     2101 gtgccaaatc catccgctat aagatggccc aaaggttgcg ctttcagcta agaaggatgc
     2161 ccgaattgag tttctttctg gacgattcac tggattatgc tcaaaacata gaagatttgc
     2221 tcaaaaaatg aatcttgcat tccatattgc ccgtcgttac cttttttcaa aaaaatcaca
     2281 caatgccatc aatattattt cgatggtttc ggtttttggg gttgcggcgg ccactatagc
     2341 aatggtttgt actctttcgg ttttcaacgg ttttcaggat ttgttgacgg gtctgtataa
     2401 aaactttgaa ccggagctta agattcgtgc ctttgaagga aaaaccttca ggctgaattc
     2461 cgattttgac cttatacgag cccataaaga ggtggctgtc ttttgtgaga gcttagaaga
     2521 gaacgccctg atcagatata aaggaagcca ggccagcgct ttggtcaaag gcgtttcgga
     2581 caatttttcc gagctggcca atatggacag ccttgtttat gccggtaaat ttgttctcga
     2641 agaagagggt tttcaatttg ccagcattgg catcggcctg gcaagtttgt tgggtaccgg
     2701 aagctccttt attgatcctg taactcttta cgctcctaaa agaacaggtt cgttgaatcc
     2761 gctgaatccg gcatcggcct ttaaaacccg gcgtatttta ctaacggcca cttttggcat
     2821 tgaccaaccc gaatacgacg accggatcat tatcgtgccc ctgaattttg ccagagaact
     2881 gttcgaatat accgatgaat tgagttccgt ggagttgaaa ctaaaaaaag gagctaacct
     2941 tcaacgggta aaagaggatt ttcgtcagct tttgggcccg gattttattg tggaagacct
     3001 aaaagaacaa aaagaagata tttaccgaat taaccgggta gaaaaatgga tcacctacct
     3061 gattttgtgt tttattttgc ttattgccct gtttaacgtg atcggctccc tgtctatgtt
     3121 gatcctggaa aaaaagcagg atgccggcat tcttaggcgt cttggtgccg atcataagac
     3181 catccgtaaa gtgtttttgt acgaaggcgt tatgatcgct tttttgggag ctttttcggg
     3241 cattttgctg ggagttttgc tttgcctgat tcagcaacat tttgggcttc tcaaactagg
     3301 gagcggtaat tttattgttg gttcctatcc tgtgcttgtt aaggcaagcg atttgttttt
     3361 tattttgctc accgtcttag ctgtcagtct gccctctgtg tggtggccgg tacattatta
     3421 ttttaaaaat gacgaacttc cttaagatcg cttatgggag aactctggtg atcacaggtc
     3481 taatctcctg gttgtttgtt ttggattttt ctcctttgca agcccagcgt tcttatggag
     3541 gcagaccttt gtccattgaa gaggaagtgg cttatgttcg agccctggcc gattatttgg
     3601 tggaattacc ttcctttgac gtagactctc tgcttcgtga ggattcgatt tatcacaatc
     3661 ccaacggggc gttgcgtttt gcccataaaa tgtttgtaaa tctgagtcct gagaattccg
     3721 gcattacctt taatctggaa gacggtacca aaatatggaa actgggcttg cgctccaaag
     3781 gagcctacag cttgaatgtg atttttgaag agtatattgt gcctccgggg gcccggctct
     3841 atttgtataa ccccgataag acacaagtgt taggatcttt tacttcggac aatatgcagg
     3901 agagtggtga atttgccatt gctccgattt ccggcgaaga aattatgata gaatatcatg
     3961 aaccggccga tgcggccttt cccggcagac tccgtctcac cgaagtgaat cacgactacc
     4021 gtggcccgat gcttttggga accaaatttg ataaattgaa tttgccctgc gtgcccgagt
     4081 tgagttgtca ggcagagtac gacagcatcg ggcgcagcat ttgcctgttg atacttaacg
     4141 gcaccactta ttgtaccgga acttttgtaa acaatacagc ctgcgacgga cgtcctcttt
     4201 tacttacggc tgctcattgc ctcgaaaaca atccctcatt ggccggcagg acagtggtct
     4261 ttatgaatta cctttctcct cgatgcatgc atcaaatcag gggttccgaa gagttttccc
     4321 tttcgggttg tcaggccctg gccttttcgg aagaaaccga ttaccttttg atggagctga
     4381 aggaaaaaat accacccgac taccgcttgt atctggccgg ctggagccgg gatacgtcag
     4441 cgcgttacga gtcacccttt gcctgccttc accatcccta cggagaagga aaacgctacg
     4501 cgcagaccac agactctttg acctatgcta cctggcctta ttccggagac ggttttgatc
     4561 ccgacagcca ctggtctgta aggcaatggc aggaaggaca cacctggacc ggctcttcgg
     4621 gagccccctt gctgaacaaa caccaccaac tgatcggatt tctgaccgga ggcgattccg
     4681 ggggaagcag cggttgcggc ccctatcagt tgggtgattt ttttgtccgc ttccaaaagg
     4741 cctgggatta cgaaagcgat agccttaacc aattggcaca ctggcttgac ccgctttcga
     4801 gcgatgcact cacgataagc ggtctggatc caaaccagct taatccggcc aggcgtataa
     4861 ccaatctcag acccacggat tctatcgccg ggttacggca tgaaaatccg gaaaacggtt
     4921 ttcttttcgg gcacaacagt tttgagtaca aaatctttgc cgaagaattc cgccaggata
     4981 gcaattccat gcttaaaggg gtgtatttga tgccttcgaa gggtgttaaa agtagctcct
     5041 atcctgttta catagacgtt atgaaaggag ggtttgtgcc ggaagaaact ttggcttcca
     5101 ttgagttgaa tccctactat ctcgaatacc gtaaccgcaa tttccatgaa acatctaaag
     5161 tgcattttgc caacgccgag aattatgtct gttttgatcc ccctctgtcg gtaggcaaac
     5221 atttttatgt ggctattcgc ttagtctatc cttccaaaac ggtggccgcc attgacagct
     5281 ttgctttgta ttccgcttat cataaggaaa gagcggccaa aaacaccgta tggttaaaca
     5341 atggaacagg gtgggagttt ttcacagcga tgaaccgcag agctcattcg agttcactgt
     5401 ggctcgaacc tgtggtaatg caagacacgc tccctgtcgt agagcctcct cttgttgtcg
     5461 atacgctcag tcaacgaatt ccgctgtatt attcttttca ggacagattg ctgcatatct
     5521 cgtttccgac gggctggcgc agcccctttg aaattcgcat tttcgacccg gccggtcgtc
     5581 tgatgcactt tacttcgacc gaacaggaaa ctatgacgtt gtacattcct gaaagtttca
     5641 aaggatttta tatcattcag ttaaccgagc gcaggcggcg atatgtgcaa aaaattgttt
     5701 tctaaagcgt gtaaatagcc aaaacacacg gctttaagga ctaaaatctg ttaaattgtt
     5761 aattttctcg cccaaatacc gaaggattca attaaatgtt tcattttaaa tgtatatttg
     5821 tgggttgaaa tttttactaa caacataata cattgatcca tgaacaaaac ctttaaactt
     5881 tcaatcctag tatccctttt gttgatttta tcgggatgcg gctctaagtt agcattagat
     5941 cgtgagcatt ttacagttac accacaggtt cttgaagccg taggtgggaa agtggatgca
     6001 accatcgatg gtgttttccc tgccaaaatg tttccggcta agggtatagt tactattacc
     6061 cctgttttga aatacgaagg tggtgaagtc gaaggagcac cgatgagctt ccagggagaa
     6121 aaggccgttg gcaacaacaa agttatacct acggctgccg gtggtaactt cagcatgaaa
     6181 tcctccttcg actacaagcc cgagatgaaa cgctcagagc tttttgtccg cattaattca
     6241 gttacgggca ataaaaccca agatcacccc gaaattaaac tggccgatgg tgtggtatcc
     6301 acagaaaatc tgttgaatgc tcaaaaggct aagccttcac ccggcgcaga caaatttcag
     6361 cgtatcattt ctgaagtaac cgaagctcaa atccttttcc ttattcaaca atccaatgtt
     6421 cgcaccagcg aactcagaag cgacgaaatt aaggccctgg tttctgctat ttcaaatgct
     6481 actcagactg aaaataaaga aatttccggc attaatgttg cttcttacgc gtctcccgac
     6541 ggccccgtta agctgaacga atctttggca ggcagtcgtg aaaaaaacac ggtcaactat
     6601 ctaaatagag aattgagaaa agccaaagcc caaggtgata tcaatgctga gtttaccgcc
     6661 gaagactggg atggatttaa gaaactgctg gaagcttccg acgtacagga caaagctctt
     6721 atactgcgtg ttttgtctat gtattccgat cctgaacaac gcgaagcaga aatcaaaaag
     6781 ttgtcgtctg cctacaaggt gttggccgat gaaattctgc ccaaactcag acgttcaaaa
     6841 atagctttga ctgttgacgt tatcggtaaa tcggacgaag aaatcaaggc gttgttcggc
     6901 tccaatccct ctgaactcaa cgtagaagaa atactttacg ctgccactct gtacagtact
     6961 cctgccgaaa aggaaaaagt ttaccaaaaa acaacgcaac tatatcccaa cgattcccgt
     7021 gcattcaaca acatagggat gataaaattt gaggccggag atgttgccgg agccaaaacc
     7081 tggttcgaaa aagcctatac gatcgataaa tccaaagaat cttctctcaa cctggccctg
     7141 tgtgccattt ccgaaggagc tgacaatgct accgtcgaag cctacctgaa tcaagctgcc
     7201 ggtgccgaaa attacgaagg agccttaggt ttgctttata tcaagaaagg tgattatgcc
     7261 aatgctgtta aagcctttgg agatcagaag agcaataacg ctgctctggc tcagattttg
     7321 gccaaagatt acagcaaagc caaagctact ctggccggag ttgaaaaacc cgatgctgag
     7381 acctattatc ttcaggctat tgtgggtgcg cgcaccaata ataaggctga aatcgtggat
     7441 ggtttgaaga agtccatagc tcaagatccg gccatggcta aaaaagcggc caacgatatt
     7501 gagtttgcaa aatatctggt cgacgccgaa gttgcaagcc ttctgagcta agcacgatac
     7561 aattccccca aagggtgctt ttaaaaatga cagaaccccg aaagttaaat aaaagacttt
     7621 cggggttttc ttatgcgcaa aaaacacatc cgcagaactg ctgtatcagc ggtctttctg
     7681 aatcaagttc agcaagcgct ccaacgcttc ttcttcggga atgttctttt cgacgcaaag
     7741 cttgcctttg tagaggctaa tccggcctct gcctgccccc acataaccat agtcggcatc
     7801 ggccatttcg cccggaccgt ttacgataca gcccattacg gcaatcttga ggcctttcag
     7861 atgagaggtg ccggctttta ctttggccag ggtgctttga agattgaaca gagtccgtcc
     7921 gcaacccgga caggagatgt attcggtttt gctgattctg gagcgggtgg cctgcaaaat
     7981 accgaacatt aacgagaggc actctgcctg caagcctcct gccgagatgc acacaccgtc
     8041 gccaaaaccg tcggctaaca aaaaacccaa atcggttgca gcctgaactt gcagattctc
     8101 gacatcttct gtggagtagg cggaagcaac aacgacgggc aaataggagc ccagatttaa
     8161 tagggtatga aaagccgccc gcatttctgc tgcggcattc aaatgggaac tgtacagcca
     8221 aagtactaga ttttgctctt tttcaagttg ctgcaataat ccttcgttca ggtctgtgtg
     8281 gcagacgcgc agaaaacgca gtgctgcctt acagcccgat aattcttcga tttgtgcgac
     8341 ttcaaataga ggatagcatt gggtctgctc tcgatatacc ctgtaatcac agatttgtgg
     8401 aatgttgtcc ttgcggggag cttcgggagt tttggttccc agatagatga aatcgggcat
     8461 agggtcggca aaagaataat ctgttgtaag cccttgtgaa tgatcttgta tgacaacagg
     8521 gacctgctcc tttccgataa ttccggcttt tttactctcg cgccgcttgt agctgtaggg
     8581 atcaaaacct tcgaaggcct tggcattgat taaagcgtgc ccttttcttt cttccacgta
     8641 ttccaccagt ttgcgagcta ccggaatttc tacctcagga tcttcgctaa gcgatacccg
     8701 cacggtatct ccaattccgt ccaacagcag gctaccaata cccacagccg atttgatgcg
     8761 tccgtcttcg ccgtctccgg cttcggttac acccaagtgc aaagggaaac tcatcccttc
     8821 ggcttccatg gtctttacca gcaagcgcac agtatggatc atagccaggg tgttggacga
     8881 tttaatggag atgactaccc gactgaaatc ttcttccacg caaatgcgca gatattccat
     8941 gcaggattcc accatgcccc ggggactgtc tccgtaacga agcatcattc tcgacgaaag
     9001 cgatccgtgg ttgacgccga tgcgcagagc ggtaccgcgt tttttgcata tagcaagcaa
     9061 aggcagcagc ctttccctga ctttttgttt ttcgagctca agaatactta ctcccccaag
     9121 atcacttacc ccgcccttgg gtagctcaat gtgcagagga tcggcaaaat tgcccggatt
     9181 gatccggact ttttctactt gggaagcagc catttcggcg gccttgggat tgaaatgaat
     9241 atcggcgatc aaagggactt caactccctg tttccgtatt tccctgcgaa tattgcccaa
     9301 attttcggcc tctttggtcc cctgtgccgt taggcgcaca taatcggctc ctgcggcgac
     9361 tatttttttt atctgttcca cacaacagac cgtgtccagt gtggaacaat tggtcataga
     9421 ctgtatgcgt acaggataag acccgcccag aggaacctga gcgatgttta ccggggagct
     9481 ttttctacgt tggtagttaa agagattccg acaataagtc attggttttg tattcgtact
     9541 taagctgttg caattcccgg tcggccttta caattttgtt tttaagtccg gttttataag
     9601 cgataagccg ggcttgcaat tcctggtctg ccacagccag tatttccacg gccagcaggg
     9661 cagcgttcgt tgctccgttg atgcctacgg tggcgacggg gatgcccgga ggcatctgta
     9721 caatggccag taaggcatcc atgccttcga gactggcttt aataggcact ccaatgacgg
     9781 gcagagtagt ggaagctgca ataactccgg gcagatgtgc agccatgcct gcggcggcaa
     9841 taattacttt gattcctctt tcggctgcgg tcctcgaaaa cttttcaact gcctcggggg
     9901 tgcgatgagc cgacagcgcg tgcatttcaa agggaatttg aaattcgttc agtaagtctg
     9961 ctgctttttc catgacaggc aagtcggagg tactgcccat aattatgctg acaatcgctt
    10021 tcataggttt atcctatcat ttctttatat tgttcataac tgagcagttc ctgcagctct
    10081 gcggaattat tcaagctgat ttttattatc cagcctgcgt cgtaaggttc tttgttgatg
    10141 agttccggat gatcttccaa ttcagcgttg gcttcgagta cggttcctcc tacgggaata
    10201 aaaaggtccg aaacggcctt gacggcctca acactgccaa aaacttcttc tttttgcaag
    10261 ctctctccta cggtcgtgat gtctacgtag acaatttctc ccagttcaga ttgtgcaaaa
    10321 tcacttatac ctacgtaggc ttcttgccct tctacacgaa tccattcgtg atttttggta
    10381 aattttaatg ttttagggtc gttcatattc ttataagtat ttaaaataga aaagctgttt
    10441 tactgtacac gcaacctggc caccgggatc ttcaactgcg aacggtattt ggaaatggtg
    10501 cgcctggcca ccctgtatcc tttgtcctgc ataattttct ccaactcgtc gtcggtgaaa
    10561 ggcttactct tgtcctcgtt gtcgatgatt tcctgcaaaa cattttttac cctgatggtg
    10621 gaaacttctt ctccggagtc ggttagcagc gattctgaaa agaaatattt caaaggaaag
    10681 attccaaaag atgtttgcac gtatttactg ctcgaaactc tcgatatggt ggaaatatcg
    10741 taagaggtga tgttggcaat atctttgagg atcatcggcc tgattttttt ttcattgccg
    10801 ctcataaaga actccttttg atagtccatg atggcctgca tggtgctgag cagggtttgc
    10861 tgtcgttgtt ttatagcctc gataaaccaa taggccgctt cgactttttg acgggcaaaa
    10921 acggctgcat ttctggtttc ctcgctttgt ttatgcggac tggcgttgta gtcctggagt
    10981 aatctaagat agcggctgct tacgtgaatc tcgggtaggt tgccttcgtt gagactgaga
    11041 acaagattac cgtcaatatt ctcgataata aaatccggaa taatctgttt tcccgattcg
    11101 cccggcattc cttcccaagc gttgccgggt ttgggattga gtttaagaat ttctccaact
    11161 attgcgtcgt attcttcacg agtgatgccc catctatgga ggatgcggtc gtggtgcttg
    11221 tgcgaaaatt cttcaaagta ctgttccagc aggtggatag cctgctccgt caaaacagtc
    11281 ttgggttttc ggtaaagttg cagcaacaga caatctttaa gattctgggc ggcaacaccg
    11341 gcgggttcga aatcctggat caccttgagc attttgtgca tctcgtccaa ggatacttcc
    11401 ttgaatgcct gaaaagaaat ctgatcggaa accgtttcga gaggaatgcg cagataaccg
    11461 tcgtcgtcga tgtttcctat gaggtattca ccgattattt tttcattttc ggtgagattc
    11521 tgcatatcaa gttgctgcat cagaaaatct cgcaaagaca aggcacttga tccaaaaaaa
    11581 gtctccctgc ggtcgggggt gtttttgtcg tattcccggt ttccgtacag atcgtcgtcg
    11641 tcttgcaaat aatcttccag gtctaattcg tcttgctgct cttctgcttc ctcctcgagg
    11701 ctctcttctt tgctttcggg atctcctccc agttccaagg caggattttc gtctaactcc
    11761 cgcaggatac gctcctccat ttccaattcg ggaatctcca gcagcttgat tctcagaatt
    11821 tgctgaggag aaagtttttg ttgaagcttt aacgaaagtt cctggtgtaa catgttgttc
    11881 cgtcaattaa aacctttgtg caaaagtagc atttttcggt tgaatcagcg gtgattcggt
    11941 taataacgaa aactgctgcc tccgacaaat tctcttagta gcgaagtggg aggaatttcc
    12001 caatcggtaa tggggacaaa gtaatccagg aagcgcagta aacgtctcgc ttcggagtat
    12061 tcgacgcaat tctgggggac ttctctcaat acgggttcta tgtaattttt taatacagag
    12121 aattcaaaga tcagtgccga attaaacatg gcatcggcat tctcctggaa ggggaaaatc
    12181 catttttctt ctccggcacg gacactatcc cagcgggcta tagtttccct agccgaagtt
    12241 ccccggtagc gggaatcccg gataattctc cggatcagcc tgttgtctgt ggttggaatc
    12301 cggttgtgtt tatccagcga aatggcggta agggctgaaa tatacaattt aaattgggct
    12361 tcaggcggaa catctttcaa caattcggga tttagtgcgt gaatgccttc cagaataagg
    12421 atgttttgcg gcgtcagtct gactttattg cctttgtatt ctttggttcc cagagcaaaa
    12481 ttaaagctgg gaatttccac ttcctgccct tcgagtatgt tgcccagatc gcgattgaaa
    12541 agatccaaat ccaaggcata aagcgattcg taatcaaatt cgcctttttc atccttaggt
    12601 gtttgctctc tttccaaaaa ataatcatcc atagaaatgg ctatagggcg tattcccata
    12661 gcaagcaatt gtacggataa acgtttgcta aaggtggttt tgcccgacga agaaggccct
    12721 gaaatcagca ctatccgaat tttatccagc cccttggcaa tggtgtcggc caactgagcg
    12781 atcttttttt cctgcagggc ttcggccacc ttgatcaact ccgttgctct gccctgacgg
    12841 cacataagat tcaaatcgcc cacatagcga agatccataa tcttgttcca acggacaaat
    12901 tctctgaaaa catcctgcat tttgggttgg ggcaccaagg gtgccagttc ggagggattt
    12961 gaccttgatg ggatgcgcaa taaataattg tccaggtagg gaatcaagtc aaaattctta
    13021 ttaatacccg atgaataggc caaagctccg taataataat cgatatgttc ccccattttg
    13081 cagtaagtgg tgtagaggct gccaaaagta ctcagcaaat caacaatgtc ttttctgcct
    13141 ctttttcgga acaattcaat aactatggag gtttcttctt ttacgctatc tataggattg
    13201 ccggcttcaa taagttcaag tacccttttc ttgatggcgt caagatcttt ccgggtgcat
    13261 tcccgcttca gatccagctc acaataatag ccgttcgaaa cggaatgttc aatactgaaa
    13321 ctgccctcgg gataaagctc gtaaatggcc gaggagatca aaaaagtcag ggtgcgtaca
    13381 tatacccgca tacccgaagc agaattcatc tcgatgaatt cgatcctttt gggattgtac
    13441 aattcatagc tcagactttc ggtcttgtta ttaactctgg ctgcaatagg ctccatgtta
    13501 agttcttgcg aaagttgttg gtaaacttcc agcaagctta cccccatggg catgagcatg
    13561 tttttgttgt tatttacaca aaaaatacga acggcttttg acatagtaat agtgaaaaat
    13621 cggctctaag gtacaattaa atttccatag attctgtaat taaaaaagtt tcacttactt
    13681 ttgtggcgca aaagcaggat ttatatttat acagcttcca tggattattc tttttatgat
    13741 ttcctcatgc tagtcggctc actgggccta ttccttttcg ggatgaaaat gatgagtgag
    13801 ggactacaaa aagcagcaag taaaaaatta aaagaattcc tttcggctat gacttcgcac
    13861 cgttacgcgg gagtcttgat gggaatgttg gtaacggcca ttatccagtc ttcttcggcc
    13921 acaacggtga tggtggttag tttcgtcaat gcggggttga taagtctggt gcagtccata
    13981 agcgttatta tgggagccaa tattggtact acaataacag catgggttat ctcggtcttt
    14041 ggatttaatt tcaacatagc ggaactctcg attcccctga ttggaatcgg tattatacct
    14101 ttgctgtttt ccaagaaaaa caatcacaaa aacattggag aattcattat aggttttgct
    14161 cttttgttta tggggctttc ttacttgaaa gattccgtgc ccgatcttca aaataatccg
    14221 gaagcgcttg aatttatatc caaatttacc caaaagggct acggctccgt tttgattttt
    14281 ttgctctttg gcaccttgct gaccattgtg attcagtctt ccagcgctac catggcggtt
    14341 accctgatta tgtgtgccaa gggatggatc agttttgaaa tgggagcggc catggtcctg
    14401 ggcgaaaaca tagggactac ggtaacggcc aacttggcag cgatttccac taatgtgtcg
    14461 ggtaagcggg cagccttggc gcataccacc ttcaacgtga tcggtgtgat ctggatgttg
    14521 attgtgtttt tcccttttgt gggcatgatc caaaaaatca tcgttaatgt aaccggtgtt
    14581 gaccccaagc aactggatgt ctttactgcc agcctggatc ctggggtgct taaattgatt
    14641 tccgaccccg gaataaccct cgacgatcct caattaataa gcttacgcag ccaattagta
    14701 tccttgcagg ccagcacatc ttacgcgctt tcgttgttcc ataccatgtt taatcttatc
    14761 aacaccggtt tgttgatttg gtttacggga acgatagcca aaatagtcac caagattatt
    14821 cccagtaaaa agaccgacga agaattccaa ttgagataca tcagcaccaa tctgctgtct
    14881 gcttcggaat tggccatacc cgaagccaaa aaagaaatcg acgattacat taccagaacc
    14941 cggagaatgt ttgatatggt gcgtgattta tactatgaaa aacaagagtc caactttgag
    15001 aagatctatg cccgcatatt gaaatatgaa agtatcagcg atagggtaga actcgaaata
    15061 gccaccttcc tgaataatgt ttcagaaggc cgtctgagcg ataaaagtaa gcatcaaatc
    15121 aggcaaatga ttcgcatgac ctccgagata gaaagtatag ccgacagttg ccacaacatt
    15181 gccaacacca tcaagaggcg ctgggacgca aaggtggttt tgaccgatga aatgaccgag
    15241 catgtcgaaa ttatgtccgg tttggtgtat aaacagttgg agggcatggc aaatctgatt
    15301 ttaaaaaata gtcacgacac tgccgaatta ctcaaaatca gagatgcgga gatagagatc
    15361 aacaaatacc gcaaccagtt gtaccaggaa aatgacctcg atctggaggc caaaaaatat
    15421 acctatcagg aggcgttgta ctatatagat attatacgcg aatgcgaaag gttgggcgac
    15481 tacattgtca atgtggtgga agcctatacg ggaggatcta aattgtttgc tcatatctaa
    15541 ataaggtcat caatttcgtc tgatccgaag tttttgtctt atctttgcaa acttttgatt
    15601 atcacggata gaatttcaac aacaaataca tcaaaaatta gaattatgaa gaaaaatgta
    15661 atgttagccg tcgtttttgc cggcaccatg tgtttaagct tatgtttcac ctcttgcgac
    15721 tgcaagtgtg gaagtaaagc aatgaaaacc aacgtagaca gccttagcta tgcctatggc
    15781 gtagggctgg gagacaattt ggcgcaaaac ctcagctctt ttcccgccga actgaatatg
    15841 gatctgtttt tggaagcttt cgagaaaacc atcaagggaa agaccgaagg tctcagcatc
    15901 cagcccgatc aggcttatca ggtattccaa agttgcttga cggccataca aatggaatcc
    15961 gctcaaaaga ataaagaaga ggccggtaaa ttcctggaag aaaacgctaa gagagaaggc
    16021 gttgtaacta ccgaaagcgg attgcaatac gaagtcctct tggcggccga aggtcccaag
    16081 cctacagcaa cagaccgagt tagtgtgcat taccacggta ctctgcttga cggttccgtt
    16141 tttgacagtt ccattgatcg cggcgaaccc gcccaatttt cgttgaacgg tgttataccc
    16201 ggatggaccg aaggcattca attaatgagc gtaggctcca agtataaatt ttatttacct
    16261 tccgatttgg cttacggtga caacggtgcc ggcgatgtta tccaaccggg cgccatgttg
    16321 attttcgaag tagaattgct cgaaatcctt aaataataga agaatgattt gtttacggta
    16381 attcgttagc cgtttttatt agcccggcaa gtttaccgga gacaaaacat acattttcag
    16441 gagtcgtctt aaagtctgct ttgagacgac tcttttactg tttaaaaaag cgcaacacgg
    16501 cctctctgtc gagtttgagc tgatccgaca gctcttcagt ggatgggaaa cgcctttcgg
    16561 gtctaaggtg ttttttgaaa tgaacggtga ttcccttatc gtagaggtcg cccgaaaagt
    16621 cgattaaatg cacctcaatg ctcctctgac agccattctg caaggtgggg cgttgtccta
    16681 tattcaacat gccgggatac agcctgtttt cgacttctgc caaaacaatg taactggcag
    16741 gcaagggtat aagtttatga ggatcggaag gaataatatt ggccgtggga aatccaagtt
    16801 tgcgacccac tccgtaaccg ccgcctactc tgcctttgat ggtgtaatcg taacccaggg
    16861 cggcgtttgc tttcaatatt tctcctttta gcagggcctc tctgatgtaa gaggaactga
    16921 tggccttgga agaatcggga agtaagaccg ggccggcttt taaaacttca atgcccagca
    16981 catcgccgta acggcgataa tcgtcgaagt cttcttcctt gtttttacca aaacggtgat
    17041 cgtaaccagc cagcaaacaa gccacgttcc attctttttt caggacctgg ttcataaatt
    17101 cgaaagccga cattcgggcc aattgtttgt cgaaacgtac aagttgggta tgatcggcgc
    17161 cacaggcccg cagcagagcc aatttttcgg aacaatcgtt caataattgc ggacaataat
    17221 cgctgtgtaa gaccattctg gggtgaggcc aaaaggccag caccagcgag gccaggtttc
    17281 tttgtgccgc ctctcgacgg agcgaggcta tcagttgttt gtgtcccagg tgaacaccgt
    17341 cgaaaaaacc gatggtggcc actgtctttt ccgctgtaac cgtgtttagc atttcgggaa
    17401 aaaacttgct caaatcctcc acgctgccgg gacaaagggt tttcattccc agggcttccg
    17461 cactgcgaca attggccggc ccgtcgtcga taagaagaca ttcttccgcc ttgagacagc
    17521 tgtctttgag cacgtgttca aaaaactctt tgtggggctt ggaaaagcca atttcgtagg
    17581 acaaatagca tttctcaaag cattggttta gaggccgccc cgcctgctca aactgctcaa
    17641 gtttacggat atgtatggga ttggtattac tcaacaaaaa gagcctgtac tttttgctca
    17701 ggttatggac aatagtcgtg ttttcgagtg gaatatcttc gagaaaagca gcccaggccc
    17761 ggtctatttc ttcgtcgccg gccttgatgc caaagattct tctgatttct ttccgaaatt
    17821 cctccaggcc gattttaccg ttttcgaaaa gaccaaacgg tccggtatgc ccgtacggcg
    17881 tgagataatc tgccacttgt ggcgctccca gcgccccaaa agcatccagg caggcctgtg
    17941 gattgagctt gaccagcacc cccccaaaat caaacaaaat attctttatt ccctttagtt
    18001 ccatcgcc
//
LOCUS       DAOSTD010000033        17770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_3310_length_17770_cov_15.907988, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000033 DAOSTD010000000
VERSION     DAOSTD010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17770
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3310_length_17770_cov_15.907988"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLI37_03685"
     CDS             <1..732
                     /locus_tag="PLI37_03685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ58152.1"
                     /translation="YLISCQGVQDPEPLDFQRKDVSISFTGSTGIAANRNNSIKAARG
                     KIVWLLDDDVRFTQSDLRRILECYSAKPEIDLACFQIRTPEGRPYKHYKEQAFRMKSL
                     DDVRDVSSIEITFRLASIRAKGLVFDERFGLGRQWPCSEEFLFLAACLKAGLHIHYFP
                     YPVVEHPYESTIKKMSQYSPVLLEKSGAVNAVLYGRKAYLRNLVSLIKARKAIKNENM
                     SACDFLRIKNRGSRKLLHSNRDERS"
     gene            719..1714
                     /locus_tag="PLI37_03690"
     CDS             719..1714
                     /locus_tag="PLI37_03690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOQ58153.1"
                     /translation="MKGPEISVFIPVYNAGAYLKPTLESVLNQSFTDFELLVVDDSST
                     DGSWLILQDYAGREPRMRILQKEHGGNVPKSWNVALPLLRGKFVFYLSQDDLLSPDCF
                     EQMIRRQRECDADCVLPDMLRFTRDPSDAVQEQALTAPDADYNRILDGRSAFLLSLDW
                     SIHGFMLCRRQFYTEEEFDESTFNSDEYVTRKIFLKSGQVAFSRGRFYYRRGNPNSIT
                     DPGHPLRYQVLRTNRRLLRLMQEQGFDRQELYKYRRQVFDTFCGLHRYYLNHKNSYVS
                     TEQSSIQALFAEEYEALHKERKATKRAYRHSGVKQRIRAALMLSSYGVFVLFNKK"
     gene            1716..2753
                     /locus_tag="PLI37_03695"
     CDS             1716..2753
                     /locus_tag="PLI37_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOQ58154.1"
                     /translation="MKHRQIILPPEWAKQAFVQLTWPHKDSDWADNLEEVEACYIEIA
                     RHILRYEPLLVVCSHPNSVRAKIAPQSGQKLFLYELPCNDTWTRDFGPISVYVDGKVC
                     IYDFGFNAWGGKFAFDLDNKLTAKLLEAGAFGPDRLYSDRKNFVLEGGSIESDGAGSL
                     LTTSECLLSPTRNPGMNRDEIELYLKQAFGLQRVLWIENAYLQGDDTDGHIDMLARFC
                     NPDTIAYVSAGFPQTAGGHEPKQLESLRAMEGELKSFRTLSGQSYRLIPLPLPDVVRY
                     KNEVLPASYANFLIINGAVLLPVYGQEKDQQAVKQLAKAFPDREIIPVNCLALIKQHG
                     SLHCISMQHPV"
     gene            2883..4256
                     /locus_tag="PLI37_03700"
     CDS             2883..4256
                     /locus_tag="PLI37_03700"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOQ58155.1"
                     /translation="MISSQRYLLFFLLMSLLLACTNTTGQYDLTYPVVRMDFSDELVL
                     EGSVEATNSTFIKCPDVYNSKIIYLVEDGAWVEAGDTVCILENQQLVNQLETVKRLCE
                     LYQAEYNKGVADLELDYALLQAQVKNNETQMAISSLDSLQLQYYTENQRKIAEIRLEK
                     ARIESRKLKKKLSALETIQRSELRKMELQIIQSQTLLESTQASLDELILKAPKAGMAI
                     RGDSDISDGKIQEGEEVYPQTPLVIIPDSGQVRIKIQATEAAYKRLALGQKVEYTFDA
                     MPGNFAYGFIVMKSPRGYTMSRNSRVKFFDVLASVDSGLQTPTAGLSANCRVFLNYLP
                     DTVAVPIVSVFQEDSINVVYIKKGSGFIRQEVSVALQSSKEAVIACGLRGGEDIAMLQ
                     PLRSKVKDEVFLDYEQRNRFKTVKNDSLPADFLEVPQHIVPRRDSPEQMEEEPTFQFQ
                     DHIIIVR"
     gene            4266..5498
                     /locus_tag="PLI37_03705"
     CDS             4266..5498
                     /locus_tag="PLI37_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HOQ58156.1"
                     /translation="MKKSILLLLPIGLLCAACGKEKKQEIPLTQVRQETFYIDLYEEG
                     DIQATQSKVISSPEISWRYGDLKITYVVDDGQEVEAGDTVVIFDPSEVRKAIVDAESR
                     LEIHYAELEKMQAQQESDMEEMMADFEVTELSLEISRLEYESAEYEAVIKKKEIELNL
                     ERATIALEQAKSQIENRKKIQKEELTQKKLIISQAKSELEDAYNTLNLLFVTSPSPGI
                     AIVSQNNTTGLKFQEGDQVWKGQPMIELPNLNELKAAIKINEVDIAKITKGQRVIIRP
                     DAFSDSLYFGHIQTVANLAVNKERNSRIKVFPVDVLINSYPTDLMPGLTVSCRIIIDE
                     IPDAVYIPLNALFKEGIQDIVYVKTATGFRIQEVKTGQSNADYIIIEEGLKAGENIAM
                     ANPFLVAEANKSSKKQAQ"
     gene            5495..6703
                     /locus_tag="PLI37_03710"
     CDS             5495..6703
                     /locus_tag="PLI37_03710"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOQ58157.1"
                     /translation="MKRTYFSLLLFGLSAGLFLLPGCKQTAQESNLIKRGDFKQTITE
                     TGELFTVDNRSIVMPRYGRYWYNMKIIGLAEHGSKVKAGDSIVQFDPTEVKKFIMDRE
                     TRLENEQANLEKLLVQQQNNLKNLQSTLRGEEASFNLRKLAMEYTKFESERVQEIKRL
                     QFRQAEINFEKVKRRIELTKVMAASDLKIQKIKVAQIENEIKMAQDALPQLTMRAPIS
                     GIFQVARKRRSRDNIAVGDDVRFGTMVGSVPDLTWMKVQTTVNEVDRAKITQGQPVIV
                     RLDALPQVAFDAQVSFISVLCRPYDNNDRRKVFDIEVKLLESDERLKPGMTVSCEFIA
                     NDLKDVLYVPNTCLLKEGGRYWIFMPKATGFDKVAVEFHTRNNTHSVISGNIAEGQQV
                     IPVAGIIDQE"
     gene            6710..7948
                     /locus_tag="PLI37_03715"
     CDS             6710..7948
                     /locus_tag="PLI37_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ58158.1"
                     /translation="MQFEEIVRVALHALMDHKFRSFLTMLGIIFGVASVIAMLSIGEG
                     AKREAIAKYQDLGVNNIIVREKKLSNAELEEVRARFSPGLSINDARVIREIVPEVTQV
                     APQAEKESEVKMGDMSAKSTVIGITPAFIEILNYETEEGSFITADHYNNSLKVCVLGA
                     GLANSFFSNQSALGKMVKVDDQWLEVVGVMKSKAVFTETVGELASRDLNNDVYLPLTT
                     FNKRFAKENLLESEIKQITVRVNSSDKLMESAALIRSILDRHHFNNQDYSIVIPFELL
                     KQEEKERQIYNILLGAIAAISLIVGGIGIMNIMLASVMERTREIGIRRAIGARKKDIM
                     SQFVVEAVAISITGGLIGVVLGVVLSFSISLFTDVTTYIRVYSIFIAFFFSVLVGISF
                     GYLPAKNAANLEPIESIRYE"
     gene            7961..8683
                     /locus_tag="PLI37_03720"
     CDS             7961..8683
                     /locus_tag="PLI37_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ58159.1"
                     /translation="MLIEIRNLVKNYVMGDIQVQALRDINLTIDRNEYVAIMGPSGSG
                     KSTLMNILGCLDTPSDGRYLFDNVDVSTLSDDELSAMRNREIGFIFQNFNLLPKMNAL
                     QNVELPLMYAGIPKAMRRERAIEALERVGLADRMDHKPTELSGGQRQRVAVARALVTK
                     PGILLADEPTGALDSKTGVEIMALFDELHREGNTLILITHEREIAEYSRRIIQIRDGL
                     IFSDEENIRSDNVEEKIYKTRG"
     gene            8694..10181
                     /locus_tag="PLI37_03725"
     CDS             8694..10181
                     /locus_tag="PLI37_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOQ58160.1"
                     /translation="MKTKTLICLLFFSLVFPHRPKAETYHLDLDQSIFIAKNKSFEML
                     SLLQDIKIAEYNLKSTTSGLKTHVNLNLTLPEYTETIRQFEDTSGIVFYSVRQLNYNG
                     SLRINQPLPTNGNIYLQTNLTNTDDLNNAKRLMNLNTRLGLTQPLDAIYGYNNIRAQL
                     IQAKLAYEQSQKQLKRADLDLVYNVTSVFYNLLSVQKRKELAQMNLERQKEAYEIAKN
                     KFAAGLIKEVEALQMEVDLAEAQNNYDLAIMDQSSAANRFKELIGIDIKDSVVLNSVL
                     EYEVVLIDPQKAVELALQNRLEIREQEIQIELGEMSIKRQKAEGMVKSSLTAYYQKVG
                     VSELGTDKGFLNSLDHSASDFMARPQNFGVGLTVSVPIIDWGQNKALVRSAESRLQKS
                     LYRQEEVRRSIEREVLDLSNEVISSLKRLQLLEKNLVIAEKSFEITLARFSNGDIASQ
                     ELALERERLNNAYTSHLSAYINYQLRMADLMRKTFYDFQNDKPIL"
     gene            complement(10183..10524)
                     /locus_tag="PLI37_03730"
     CDS             complement(10183..10524)
                     /locus_tag="PLI37_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58161.1"
                     /translation="MKESTPVLELNIDDFKLSDQVNATATRVQIFGVDWEALFNSKTG
                     YISPAVPVLGGFLPPLGDKAIYKLMEENPGYDVVIYPQFETKVKRFGLVGLIYREITV
                     KATARLGKLKK"
     gene            complement(10735..11307)
                     /gene="ruvC"
                     /locus_tag="PLI37_03735"
     CDS             complement(10735..11307)
                     /gene="ruvC"
                     /locus_tag="PLI37_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOQ58162.1"
                     /translation="MSTPPIIKERIILGIDPGTTIMGYGVIRVLNNKPEFVSMGILQL
                     NKYEDHYLRLQKIFERCIGLIDEYLPDELAIEAPFFGKNVQSMLKLGRAQGTAIAAAL
                     SRDIPIFEYAPLKIKMAISGNGRASKEQVADLLQRFLGISSDQLLPQLDATDGLAAAF
                     CHYLQSNRPLYEKPYKSWKDYIRKNPGSCH"
     gene            11571..11644
                     /locus_tag="PLI37_03740"
     tRNA            11571..11644
                     /locus_tag="PLI37_03740"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11605..11607,aa:Ala,seq:ggc)
     gene            11985..12314
                     /locus_tag="PLI37_03745"
     CDS             11985..12314
                     /locus_tag="PLI37_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58163.1"
                     /translation="MNKITSFFLLACSLALNGHSQVVSIDNSFGQNGKTIIPNTTEIG
                     FFDFYKQGNIIASGIAINDAGKYNITMAKTNADGVPDESFGSGGLAKISGYDHSSPIG
                     LSITDDN"
     gene            12321..13124
                     /locus_tag="PLI37_03750"
     CDS             12321..13124
                     /locus_tag="PLI37_03750"
                     /inference="COORDINATES: protein motif:HMM:NF028473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58164.1"
                     /translation="MIGNFTKVQFQGYETLIVRFDENGMLDEHFGDSGRINFDAAGDV
                     VLLNCESNDFMLLGGIESERKEIGENVNVTTTGYYISKYDYYGNLDENFGDTGKAHLP
                     KGLCPGAVKTLNDGSIAVAGAYNCLSDDELGLCKLTPAGQLDTVFANDGIWHWNTMPV
                     FDLSYEGFCGILEDSSGNLILSGTGLHTNESGWKDTDFLCNFSAEGIPDTDFGGNGFY
                     WFDFLEISGKLFKIGDNYVCIMMNADCAFKIHYVNADGSFGDYLLKFRK"
     gene            complement(13128..14585)
                     /locus_tag="PLI37_03755"
                     /pseudo
     CDS             complement(13128..14585)
                     /locus_tag="PLI37_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            14968..15501
                     /locus_tag="PLI37_03760"
     CDS             14968..15501
                     /locus_tag="PLI37_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOQ58165.1"
                     /translation="MEFSALVKLRQSDRKYEARGVEKEKIIRCLETARLAPSANNSQP
                     WKFVVVDQPELKEQVAECAVSVELNKFTHQAPVIVAVVQEKVNMLSGMGSVIQDKEYA
                     LLDLGIAVNQFCLQAAELGLGSCIVGWFNEAKVKSLLNVPAKKRVPLLITLGYSVAKT
                     REKSRKKLEEISNWNSY"
     gene            15511..16212
                     /locus_tag="PLI37_03765"
     CDS             15511..16212
                     /locus_tag="PLI37_03765"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOQ58166.1"
                     /translation="MKNTARLLYGIALTAAIYFATLLLSRLIRNIDGLVINSYLTQAL
                     ILSCLSVAAILSMKKHLSYRIALPKFKLILRPIIFTIITYVGFSLFLSLLANLIGVSR
                     GEVHPAIKLNPVQFLLFVVIAASIAEELLFRGFLLNLLAPLKQRGIKILNRKISLSVM
                     ISALLFGLAHLSLASFGYSPYFLIRTVVFTCCIGLIAGYYQEKYNNNAYAITVHMTSN
                     LVGILPVIMLAMQKA"
     gene            complement(16270..16491)
                     /locus_tag="PLI37_03770"
     CDS             complement(16270..16491)
                     /locus_tag="PLI37_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58167.1"
                     /translation="MIGGISTEEQYVKTVQKYKYPWLLTAEINDENKLWEKYCVSGGG
                     GGQFLMDNKGKIIAINPSLEDIKRILPEE"
     gene            16752..>17770
                     /locus_tag="PLI37_03775"
     CDS             16752..>17770
                     /locus_tag="PLI37_03775"
                     /inference="COORDINATES: protein motif:HMM:NF033591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOQ58168.1"
                     /translation="MNLARIKAMSLMICALCKVQRVAYTKLASAFDTDATAGSSLRRI
                     QRLIAECVIDTDLIAKLILKLIPVKSPYNLAMDRTNRKYGNTNINILTLGIIYDGMAF
                     PVVYTMMDKRGNSNTRERIKLVNRFKRIAGKDSIAHLMADREFVGEECLGYLNACGIH
                     YHIRIRENFHVIRHGRKAKAFWLFNDVKLGESKHLDGIYYVNGQACYLSGSKIKDKKG
                     KPELQILVSYCNAEKALEMYKLRWQIETLFKGLKSSGFNIEGSHVRNLDRMSNLFAII
                     MVAYVWCYLVGIYIHENIKQIKVLKHGRRAVSLFKYGLDYICQCLVNHTNRYRINVFK
                     FLSYT"
BASE COUNT         5038 a         3773 c         4093 g         4866 t
ORIGIN      
        1 tatcttattt cctgtcaggg agttcaagac ccggagcctc tcgattttca acgtaaagat
       61 gtctccatca gttttaccgg cagcacgggg attgccgcca atcgcaacaa cagcatcaag
      121 gccgcaaggg gcaaaatcgt ctggttgctc gacgacgatg tgcgctttac acagtccgac
      181 ctccggcgta ttcttgaatg ttacagtgca aagcccgaaa ttgacctggc ctgtttccaa
      241 atcagaaccc ccgaaggcag gccctacaaa cactacaaag agcaagcctt cagaatgaag
      301 agtctcgacg atgttcggga tgtaagctct atagaaatta ccttcaggct ggcctccatc
      361 agggccaaag gtcttgtttt tgacgaacgt ttcggactgg gcaggcaatg gccttgcagc
      421 gaggaattcc tgttcctggc ggcctgcctc aaagcaggtc tgcacattca ttattttcct
      481 tatccggtgg tagaacatcc ttacgaaagc accattaaaa aaatgagcca atacagcccg
      541 gtgttgctgg aaaaatcggg ggccgtcaat gcggtgctct acggaagaaa agcttatttg
      601 agaaacttag tttcgctgat caaagcccgc aaagccatta agaacgaaaa catgtcagcc
      661 tgtgattttt tgcgtatcaa aaaccgcggc agtcgtaaac tccttcattc aaaccgagat
      721 gaaaggtcct gaaatttcgg tctttatacc ggtttacaac gccggcgctt atctgaagcc
      781 gaccttggaa tccgttttga atcaaagctt caccgacttt gaactgctgg tcgtcgacga
      841 cagttctaca gacggcagtt ggcttatatt gcaggattac gccgggcgtg aaccgcgaat
      901 gcgaatactt caaaaagagc acggagggaa tgtgcccaaa tcctggaatg tggcgcttcc
      961 gctgctgagg ggcaaatttg tgttttatct ttcacaggac gatttgttgt cgcccgattg
     1021 ctttgaacaa atgatccgtc gtcagaggga atgcgatgcc gattgcgttt tgcccgatat
     1081 gctgcgcttt acccgggatc cctcggatgc tgttcaggag caagctctta cagcgcccga
     1141 tgccgattac aatcggatac tcgacggacg ctctgccttt ttactcagcc tcgactggag
     1201 tattcacggt tttatgctct gtcgcaggca gttttacacc gaagaagaat tcgacgaaag
     1261 cactttcaac agcgatgaat acgtcacccg gaagatcttt ttaaaaagcg ggcaggtggc
     1321 tttttccaga gggcgttttt attacagaag aggcaatccc aacagcatta ccgatccggg
     1381 acatcccctt cgttatcagg tgctcagaac caatcgacga cttttgcggt tgatgcagga
     1441 gcagggcttt gacaggcaag aattgtacaa ataccgccgg caggtcttcg atactttctg
     1501 cggtctgcac cgttattatc taaaccataa aaacagctac gtttccacag aacaaagcag
     1561 cattcaagcc ctttttgcag aggaatacga ggcattgcac aaagaaagaa aagcgacgaa
     1621 aagagcctac cggcattcgg gcgttaaaca aagaattcgg gcggctttga tgctaagttc
     1681 gtatggagtg tttgttttat tcaataaaaa gtaacatgaa acaccggcaa atcatattac
     1741 ctcccgaatg ggcaaaacaa gcctttgttc agctgacctg gccgcataaa gacagcgatt
     1801 gggcagataa tctggaagaa gtagaagcct gttatattga aattgcacgg catattcttc
     1861 gctacgagcc cctgttggta gtatgcagcc acccaaattc agtgcgggca aaaattgctc
     1921 cacaaagcgg gcaaaagctt tttttgtatg aacttccctg caacgatacc tggacccggg
     1981 atttcgggcc catttccgtc tatgtcgacg gtaaagtttg tatatatgat tttggtttta
     2041 atgcatgggg tggaaaattt gcttttgatc ttgacaacaa actgactgcc aaacttctgg
     2101 aggccggagc ctttggtccg gaccgcctct actccgaccg gaagaacttt gtgctcgaag
     2161 gcggcagtat agaatcggat ggagccggca gcttgctaac cacctcagaa tgcctgttgt
     2221 ctcccacacg caatccggga atgaacagag atgaaattga gttgtattta aaacaagcct
     2281 tcggtctgca acgggtgctt tggatagaaa acgcctattt gcagggagac gataccgacg
     2341 ggcatatcga tatgctggcg cgtttttgca acccggatac catcgcttat gtttcggccg
     2401 gttttcctca gacagccggg gggcatgaac ctaagcaact tgagtccctg agagccatgg
     2461 agggagaatt aaaatcgttc aggacacttt cgggacagtc gtaccgcttg attcccctgc
     2521 ctttgcccga tgtcgttcgg tataaaaacg aagtattgcc ggccagttat gccaattttt
     2581 taatcattaa cggagccgtt ttgttgcccg tgtacggaca agaaaaagat caacaggctg
     2641 taaaacagct tgctaaagct tttcccgatc gggaaattat tccggttaat tgcctggcgt
     2701 tgatcaaaca gcacggctcc ttgcattgca taagtatgca acatccggta taagaaaggc
     2761 gaagatatgt ataaacattt gagatattta tatcttttta agactattct taggagacgt
     2821 tttaacttaa tgcaaggctg ttatgtaaaa ttcatttagc tttgcctgtc catatttcac
     2881 caatgatttc gtcacaacga tacttgctgt ttttcctatt gatgagcctg ttgttggcct
     2941 gcacaaatac aacagggcaa tacgatttga cttatcccgt ggttcgcatg gatttttcgg
     3001 acgaattggt gcttgagggt agtgtcgaag caacgaattc cacctttatc aaatgcccgg
     3061 atgtttacaa ctctaagata atttaccttg tcgaagacgg ggcctgggtt gaagccggcg
     3121 acacggtttg catactggaa aatcagcaat tggtcaatca actggagacg gttaaacggc
     3181 tttgcgaact atatcaggca gaatacaaca aaggggtggc cgatttggag ttggattacg
     3241 ccttattgca ggctcaggtc aaaaacaacg aaacgcaaat ggccattagt agtctggatt
     3301 ccctgcaatt gcagtattac actgaaaatc agcgaaaaat agcagaaatt cgtttggaaa
     3361 aggccaggat agagtcccgt aaactcaaga agaaactaag tgccttagaa accatccagc
     3421 gctcggaact tcgtaagatg gagttgcaaa tcattcagag ccaaacgctg ctcgaatcga
     3481 cccaggcttc gctggacgag ctgattttga aggctccaaa agccggcatg gccataaggg
     3541 gcgattccga tattagtgac ggcaaaatac aggaaggaga agaagtgtat ccgcaaacgc
     3601 ctttagtgat tattcccgat tccggccagg taaggataaa aatacaagcc actgaggcag
     3661 cttataagcg attggctctt gggcagaaag tagaatacac ttttgacgct atgccgggga
     3721 attttgctta cggattcata gtgatgaaat cccccagagg ttataccatg agccgcaatt
     3781 ccagggtgaa gttttttgat gtgctggctt cggttgacag cggtctgcaa acgcccacag
     3841 ccggtttatc ggccaactgc agggtctttt tgaattattt gcccgatacc gtggctgttc
     3901 ccattgtttc ggtttttcag gaagacagca tcaatgtggt gtacataaaa aaaggttccg
     3961 gttttattcg acaggaagtg tcggtagctc tgcagtcgtc caaagaagct gtaatagcct
     4021 gcggtctgcg gggtggggag gacattgcta tgttgcaacc tctcaggtcg aaggtcaaag
     4081 acgaagtttt cctggattat gaacagcgta atcgctttaa aaccgtcaaa aacgacagtt
     4141 tgcctgcgga ctttttggag gtgccgcaac acatcgtacc acgacgggat tctcccgaac
     4201 agatggaaga ggagcctacg tttcagtttc aagatcatat tattattgta agatagtcct
     4261 acaggatgaa aaagagtatt ttattattat taccaatcgg tcttttatgt gccgcctgcg
     4321 gcaaagaaaa gaaacaggaa attccgttga cccaggtcag acaggaaaca ttctacatag
     4381 atttgtacga agaaggagat attcaggcta cgcagtccaa ggtgatttct tcccccgaaa
     4441 tatcgtggcg ttacggcgac cttaagataa cttatgtggt ggacgacggt caggaagtgg
     4501 aagctggcga caccgtggtt atttttgatc cctccgaagt gcgcaaggcc atcgtagacg
     4561 ccgagtcgcg cctggagatt cattatgcag agctcgaaaa aatgcaggct caacaggaat
     4621 cggatatgga agaaatgatg gccgatttcg aagtgacgga attgtcgctc gaaatttccc
     4681 gtctcgaata cgaatcggcc gagtacgaag ccgtaatcaa gaaaaaagaa atcgaactca
     4741 atctcgaaag agccaccatt gctttggaac aagcaaaaag tcagatagaa aatcgcaaaa
     4801 aaattcagaa agaagagcta actcaaaaaa aactcatcat aagccaggca aagtctgaac
     4861 tggaagatgc ctacaatacc ttgaacttat tgtttgttac ttccccttcg ccgggcattg
     4921 ccattgtaag tcaaaacaac accacaggac ttaaatttca ggagggcgat caggtatgga
     4981 aaggtcagcc tatgatagaa ttacctaatc tgaacgaatt aaaagcggcg ataaaaatca
     5041 acgaagtgga cattgctaaa atcaccaagg gtcaacgagt gattatacgt cccgatgctt
     5101 tttcggattc cttgtatttt ggccacattc aaaccgtagc caatctggcg gttaacaaag
     5161 aacgcaactc cagaatcaag gttttccccg tggatgtgct tatcaacagt tatccaaccg
     5221 atttgatgcc gggccttacc gtgagttgcc gcattatcat cgatgaaatt cccgatgccg
     5281 tttatattcc tttgaatgct ctttttaagg aaggtatcca agacattgta tatgtgaaaa
     5341 cagctaccgg gtttaggatt caggaagtca aaacggggca gtccaatgcg gattacatta
     5401 ttatcgaaga ggggctcaaa gccggagaaa acattgcgat ggccaatcct ttcctggtgg
     5461 ccgaagccaa taaatcttcc aaaaaacagg cacaatgaaa cgtacatatt tttccctgct
     5521 gttgtttgga ctgagtgccg gtttattcct gttgccgggt tgcaagcaaa cggcacaaga
     5581 gagtaacctg ataaaacggg gcgatttcaa acaaaccata accgaaaccg gcgaattatt
     5641 tactgtcgac aaccggtcta tagtgatgcc ccgctacggc aggtattggt acaacatgaa
     5701 aattatcggc ttagcggagc acggatcaaa ggtaaaagcc ggcgattcca tcgttcagtt
     5761 cgatcctacc gaagtgaaaa aattcatcat ggatcgcgag acccgcttgg aaaacgaaca
     5821 ggctaatttg gaaaaattgc tggttcagca acaaaacaac ctgaaaaatc tgcagtccac
     5881 cctgcgtgga gaggaagcct ccttcaacct gcgcaagctt gccatggaat acactaagtt
     5941 tgagtcggag cgggtgcagg aaattaaaag attgcaattt cgtcaggccg aaatcaattt
     6001 cgaaaaagta aaaagacgga ttgaactaac caaggtaatg gcagccagcg atcttaaaat
     6061 acaaaagatc aaagtggcgc aaatagaaaa tgaaatcaaa atggctcagg acgctctgcc
     6121 gcaactcacg atgcgtgcgc ccatttcggg catattccag gtggcccgca aacggcgttc
     6181 gcgcgacaat atagccgttg gagacgatgt gcgtttcggc acgatggtgg gcagcgtccc
     6241 cgatctgacc tggatgaagg ttcagacaac ggtcaacgaa gtagaccggg ctaaaatcac
     6301 acagggacag cctgttattg tgcgcctgga cgccttgccg caagtggctt tcgatgctca
     6361 ggtgtctttc atcagtgtgt tgtgtcgtcc ttacgacaac aacgacagaa gaaaggtgtt
     6421 cgatatagaa gtaaaacttc tcgaatcgga cgaaaggcta aaacccggaa tgaccgtcag
     6481 ctgcgaattt atagccaacg atctgaagga cgtgttatat gtacctaata cttgtttgtt
     6541 gaaggagggc ggacgttact ggatatttat gcccaaagcg accggatttg ataaggtggc
     6601 cgtagaattt catacccgca acaatacgca ttccgttatc agcggcaata ttgccgaagg
     6661 ccagcaggtg attcctgttg ccggaattat agatcaagaa taaaatacta tgcaattcga
     6721 agaaatagtg cgtgtggctc tgcacgcctt gatggatcat aaattccgtt ctttcctcac
     6781 catgttgggc atcatctttg gggtggcatc ggttattgcc atgctttcca taggtgaagg
     6841 tgccaagagg gaagccatag ccaagtacca ggacctgggg gtaaacaata ttattgttcg
     6901 cgaaaagaag ttgtccaatg ccgagttgga agaagtacgg gccagatttt ctcccggttt
     6961 gtctatcaac gatgccagag tgatcaggga aatagttcct gaggtgacac aggtggcccc
     7021 tcaggccgaa aaagaatccg aagtaaaaat gggcgatatg tccgccaaat ctacggtgat
     7081 tggtattacg ccggctttta ttgagatttt gaattacgaa accgaagaag gcagctttat
     7141 taccgcagac cattataaca acagccttaa ggtttgcgta ttgggagccg ggctggcaaa
     7201 ctcctttttc tccaatcaaa gtgctttggg taaaatggta aaagtggacg atcaatggct
     7261 cgaagtagtg ggagtaatga agtccaaggc tgtttttacg gaaacggtag gggaactggc
     7321 ctcgcgcgat ttgaacaacg atgtctattt acctttgacc actttcaaca agcgttttgc
     7381 caaagaaaac ctgctcgaga gcgaaatcaa gcaaatcacc gttagggtga acagctcgga
     7441 caaactgatg gaaagtgccg ccctgatacg cagcatcctt gaccgtcatc atttcaataa
     7501 ccaggattat agcatagtga ttccttttga gcttctgaag caggaagaaa aagaacggca
     7561 gatttacaat attctgctgg gtgccatcgc tgccatttct ttgatagtag gagggatagg
     7621 tattatgaat attatgttgg cctcggttat ggagcgaacc cgcgaaattg gtatccgccg
     7681 ggctatcggt gcccgcaaaa aggacattat gagccagttt gtggtcgaag ctgttgccat
     7741 cagtattacg ggaggcctca tcggagtggt gctgggtgtg gttttgtctt tttccatttc
     7801 cctgtttacc gatgtaacca cttatatcag ggtgtattcc atatttattg cttttttctt
     7861 ttctgtattg gtgggtatca gcttcggtta cctgccggcc aaaaatgcag ctaatcttga
     7921 acctatcgag tcgatacgct acgaataatg aaaacgcttt atgctcattg aaataaggaa
     7981 tttagtcaaa aactatgtta tgggcgacat tcaggtgcag gcccttaggg atataaacct
     8041 gaccatagac cgcaacgaat atgtggccat aatggggccc tcgggatcgg ggaagtctac
     8101 gctgatgaat attttgggtt gccttgatac acccagtgac ggccggtatt tgtttgacaa
     8161 tgtagacgtc agtactctca gtgacgatga gttgtctgct atgcgcaacc gggagatcgg
     8221 ttttattttc caaaacttca accttttgcc caagatgaac gctttgcaaa atgtggagtt
     8281 gcctttgatg tatgcaggca tacctaaagc aatgcgtaga gaaagagcca tcgaggcgct
     8341 cgaacgagtc ggtctggccg accgtatgga tcataaaccc accgaactca gcggtggaca
     8401 gcgacagaga gtggctgtag ccagagcctt ggtgaccaaa cccggcatct tgttggccga
     8461 cgaacccacc ggcgccctcg actccaagac cggagtggaa atcatggcct tgttcgatga
     8521 attgcaccgt gagggcaaca ccctgattct gatcacacac gaaagggaaa ttgccgaata
     8581 cagccgcagg attattcaga ttcgcgacgg tctgattttt tcggacgaag aaaatattcg
     8641 ttcggacaac gtggaagaaa agatttataa aaccagagga taaattattg ataatgaaaa
     8701 caaaaacact tatttgccta ctgtttttca gcttggtttt ccctcatcga cccaaggctg
     8761 aaacctatca tctggacctg gatcaaagca tttttattgc caaaaacaaa agctttgaaa
     8821 tgcttagttt gttgcaggat attaaaattg ccgaatacaa cctcaagtcg accaccagcg
     8881 gattaaagac gcacgtgaat cttaatctga ccttgcccga atacacggaa accatccgtc
     8941 agttcgaaga cacctcgggt atcgtctttt attcggtgcg acaactcaat tacaacggat
     9001 ctctgcgtat aaaccagcca ttacccacca acggcaatat ttacctgcaa accaacctga
     9061 ccaataccga cgatctgaac aacgcaaagc gcctgatgaa tctcaatacc cgtctgggtt
     9121 taactcagcc gctggatgcc atttacggct acaacaatat ccgtgcgcaa ctcatacaag
     9181 ccaaattggc ttatgaacag tcgcaaaagc agcttaaacg tgccgatctc gatttggttt
     9241 acaacgtaac cagtgtgttt tacaatttat tgtcggtaca gaaaaggaag gaactggccc
     9301 aaatgaacct tgagagacaa aaagaggcct acgaaatagc caaaaacaaa ttcgcggccg
     9361 gtttgatcaa ggaggtagaa gccttgcaga tggaggtaga tttagccgaa gctcaaaata
     9421 attacgacct ggccattatg gatcagagtt cggctgccaa ccgttttaaa gaattgattg
     9481 gcattgatat caaagacagt gtggtgttga attcagttct cgaatacgaa gtggtgctta
     9541 tcgatcctca aaaagccgta gagttggctt tacaaaaccg cctcgaaatc cgcgaacagg
     9601 agattcagat tgagctcggc gagatgagca tcaaacgtca aaaagccgaa ggtatggtca
     9661 agagcagcct gacggcatac taccaaaaag tgggtgtcag cgaactgggt accgacaaag
     9721 gctttttaaa ttcactcgat cattctgctt ccgactttat ggccaggcct cagaattttg
     9781 gggtgggact tacagtcagc gtgcccatca tcgattgggg ccaaaacaaa gccttggttc
     9841 gctctgctga atcgcgtctt cagaaaagcc tttatcgtca ggaagaagtg cgtcgcagca
     9901 tagaacgcga agtgctcgac ctttccaacg aggttatcag cagccttaaa cgtttgcaat
     9961 tgctcgaaaa aaacctggtg attgccgaaa agagtttcga gataaccctg gcccgtttct
    10021 ccaacggcga catcgctagc caggaattag ctctggagcg cgaaaggctc aacaacgctt
    10081 acacctcgca cctgtcggcc tacatcaact accagttgcg catggccgac ctgatgcgta
    10141 aaacttttta cgacttccag aacgataagc cgatacttta ggctattttt tcagtttccc
    10201 caggcgggct gtagctttga ctgtgatttc tctgtagatt aaacctacca ggccaaagcg
    10261 tttgactttg gtttcgaatt gcgggtaaat tacgacgtcg taaccgggat tttcttccat
    10321 caatttataa atggctttat cgcccaacgg aggtaaaaag cctcccaata ctggaacagc
    10381 cggagaaata taaccggttt tactgttgaa aagggcttcc cagtctacgc cgaaaatctg
    10441 taccctggtg gcagtggcgt ttacctggtc cgaaagttta aaatcgtcga tgttgagttc
    10501 caacacagga gtgctttctt tcatcagata ggcttgtgat ttgcagctgc taaacactag
    10561 caggccggca gccagaacga caaataatag attgtttttc ctaaaaattg gtcaattagc
    10621 tgattagtaa ttttttgatt tggtagttta attgtaatgc ggatgattct tgtagagccg
    10681 attatggagc ttattttgaa gaagaagttt attatttatt gataaagcca atgtttagtg
    10741 gcagctgccg ggattttttc ggatataatc cttccaactc ttgtatggtt tttcgtacaa
    10801 aggtcggttg ctttgcaaat aatgacagaa ggcggcagcc agtccatcgg tagcatccag
    10861 ctgaggcaag agctgatcag aactgatacc caggaagcgc tgcagcaaat cggcgacttg
    10921 ttccttggag gcccggccgt tgccgctgat ggccatcttt atttttaagg gcgcatattc
    10981 aaaaatgggg atgtctctgg ataaagctgc agcaatggca gtgccctgag ctcggcccag
    11041 tttgagcatc gattgcacgt ttttgccgaa aaaaggcgct tcgatggcca gctcgtctgg
    11101 caggtattca tcaatcaaac cgatacatcg ttcaaagatt ttttggagcc tcaggtagtg
    11161 gtcttcgtat ttgttgagtt gcaatatgcc catgctgaca aactcgggtt tgttgttgag
    11221 cactctgatg acaccgtagc ccataatcgt tgttccggga tcgattccta aaatgatgcg
    11281 ttctttgata ataggcggtg ttgacatact gttgagtgaa attctgttta gttaaacgct
    11341 gttggtcgaa atgtgattgg ttaagagcag ttgcacaaaa cgcctgactc gaattgcctg
    11401 ctttgctttt tcccctgagc gttttttaca cctgctgtct gcgaaggtaa gacaaaaatg
    11461 acaactttta cgatttgggc ttaaataaat aggcgcaggg ctgatttttt tatactcttt
    11521 gtttatcgga attgaaattt atactatctt tgccgccggt ttacccgcaa ggggcattag
    11581 ctcatctggc tagagcgcaa cactggcagt gttgaggtga gcggttcgag tccgctatgc
    11641 tccacaaact ttataacccg ctataaaaca gcgggttatt ttttgccgta taggtaaaga
    11701 aacttcaaag aaccttcaaa aaaatagttg aaatacaata aattatgtat ttttgccaga
    11761 cctgtgtcgg gaaaaaggaa tcggttttca actattgttt ggaatgttgg ggtgcaagag
    11821 aattgatgct tgggattggc tcaaagcctg cgcgggcaat tgcgcaattg gataaggaga
    11881 taattaattg attaattaag attagagaat cagacaatat caagagaacc agacaataca
    11941 atacaataca atacaataaa tcccggagct atgggtaaat tagtttgaat aaaattactt
    12001 ccttcttttt gttggcctgt agcctggcct taaatggcca ttctcaggta gtttcgattg
    12061 acaacagctt tggacagaat gggaaaacaa ttattcccaa taccaccgaa atcggctttt
    12121 ttgattttta taagcagggg aacattattg cttccggcat cgcaataaac gatgctggaa
    12181 aatataatat aacgatggca aaaaccaatg ccgatggagt acctgatgaa agtttcggta
    12241 gcggaggcct ggcaaaaata agcggttacg accatagttc tcctattggt ttaagtatta
    12301 ctgacgacaa ctaaatcgtg gtgattggaa attttaccaa agttcagttt caaggctatg
    12361 aaacgctaat tgttcgattt gatgaaaacg gaatgctcga tgaacatttt ggtgacagtg
    12421 gaaggatcaa ttttgatgct gcaggagatg ttgtattgtt aaattgtgaa agcaacgatt
    12481 ttatgcttct tggaggtatt gaaagtgaaa ggaaagaaat cggcgaaaat gtgaatgtta
    12541 caactacagg ctattatata tcaaaatacg actactacgg gaatttggac gaaaacttcg
    12601 gcgatacggg gaaagcgcat ttgcccaagg gcctttgtcc aggtgcggta aaaactctca
    12661 acgacggttc aattgctgtt gccggagcgt acaactgtct ttcggatgat gaattaggct
    12721 tatgtaaatt aactcccgcg ggacaattgg acaccgtttt tgccaacgat ggcatttggc
    12781 actggaatac catgcctgtt tttgatttaa gttatgaagg cttttgtggg attttggaag
    12841 atagtagtgg aaatcttatt ctctcgggta cgggactaca tacaaatgag tccggttgga
    12901 aagatactga ctttttatgc aatttctctg ccgagggaat accggacacc gattttggtg
    12961 gaaacggttt ttattggttt gattttttgg aaatctccgg taaattattc aaaataggag
    13021 ataattatgt gtgcattatg atgaatgccg actgtgcctt taaaattcat tacgtgaatg
    13081 ctgatggtag ttttggtgat tatttactga agtttcgcaa gtaaaattta agctgtcagt
    13141 ttaagttcca acctgtcaaa atttatataa tgtttgaacg tatcactttt gttgatcaaa
    13201 gcatccataa ttctatcgtc ctcggcttca aagacttcgg cgataattct gacaagctcc
    13261 tgaagaactc cccaaatgcg ttcggtgaca gaaagttgta ttactccctc tgtagcttga
    13321 ttaaaaagtc ctccaatagt ctgataggca gcaaaacgtt ttaccgtact caggatattg
    13381 tactgaagca aggtaataga gatgcttgca atttgactgg caaagtccct gcattgcgat
    13441 tttcccagtc cgagaaggcc ctttgcctct ttgaagaaaa cctccacggc ccatcttttg
    13501 gaatatatcc ggtaagcttc cataaaatcc aatttgtaat tagtggtcag caaacctttc
    13561 caatctgcgt ttttaccacg acggcaaaaa taaagtctga cccgtactcc tgccagttca
    13621 acatcggcca cgccataata gcaatggagc ttacggctat actttatttc ttttctggct
    13681 tgaagcaagc ggataagttc cctggcactg cgttgcttct tttcaaacag gtacttggtt
    13741 ttacccagct ttatcatgcc caggtaatga caatccatat gcctggattt gataaactta
    13801 acaatgtctg tacaggtaaa ccaactgtct gccaacaaat aatcaaagcg tatcccctga
    13861 ccaatggcct gttttaccat ccttatggct ttttgtattt tgttttgcgt atactcgtcg
    13921 atccgttcgt ttatggcctc catgagggaa cgatctttat ggtattgttt gtcgcgttct
    13981 tttggcgtca taccaaaagc tttctcagca cgttttcctt cttctccctg aagtgaaaaa
    14041 tccagcactg tctgtgtctt gccatcggtg cggcaaagga acagcgcttt aaagccaagc
    14101 agggaagaat gactcacatg agagaaaatc cgaccgatct tctcgatttt tttacccgcc
    14161 tttggcaaat cggtatcatc aataatcaga caaacagggt ccttgccttc tgggtttttg
    14221 cttcgcaaaa taattttgtt aagcaattac agattaattc gataaacaat tttacgccag
    14281 tcaatagccc catttaacag gaaacgataa aatacatcct ttttgcagga tacgaatttg
    14341 gccagacagg acgatgtata atgagaaaca tttttaacca tgaataaggg aaacaacaac
    14401 aagagttcga aaacctgcaa acgagtgtat ttgcaattcc agtggctctc taatgccaga
    14461 gtgtcttcgg atagtttaag gtgtttaata acctccatga ttgttcttat tgctttgctg
    14521 tcgtcatttt tcgtaaaaaa tgtgctgatt tctgagaata atcttgtact ttcgtgtctt
    14581 ggcattgggt gcggtatttg ttgttttgtc acctcaaata tactgatttt cagatgattc
    14641 tgaaaatata cccaatgctt ttttagttta aatagtgact ttttttactt gcgaaacttc
    14701 agttattgat taattcgttg agttcctcca ttatttgtaa agccctctcc gatttaagat
    14761 cttccaaatc cattgttttg ttgataattt tttccttgtc catactatca gtggcaaata
    14821 ttgaattttt tgcatagtta gttgaaataa taagaataag cacacaaagt cgtctgattt
    14881 ttatactcag cccctaatcg gcaagagccg tatttttttg tatgtttgcc tgattaataa
    14941 attcaattca atcaaaacac aatcaatatg gaattctcag cattagtgaa acttcggcaa
    15001 agcgaccgga aatacgaggc gaggggggtg gaaaaagaaa aaatcatccg gtgcctggag
    15061 acggcccgtc ttgcgccttc ggcgaataat tctcagccct ggaaatttgt ggtggtcgat
    15121 cagcccgaat tgaaggagca ggtggcggaa tgcgctgtga gtgtggaatt gaataaattt
    15181 acacatcagg cgcctgtaat tgttgcggtg gtacaggaaa aggtgaatat gctatcggga
    15241 atgggctctg tcatacagga caaagaatac gccctgctcg atctgggcat tgccgtgaac
    15301 cagttttgcc tgcaggccgc cgaactgggg ctgggtagtt gtatagttgg ctggttcaac
    15361 gaagccaaag tcaaaagcct cctgaatgtt cctgccaaaa aacgagtgcc tttactcatc
    15421 accctgggtt attcggttgc gaagaccaga gaaaaatcca gaaaaaagct ggaagaaatt
    15481 agcaactgga attcatattg atccataacc atgaagaaca ctgcaagatt gctttacgga
    15541 atcgccctga cggcagctat atattttgca accctattgt tgagtcggct aatcaggaat
    15601 atcgatggct tagtcattaa ttcttacctg actcaggctc ttattctgtc ttgcctgtcg
    15661 gtcgcagcca ttctatcgat gaaaaagcac ctgagctacc gcattgcact accgaaattc
    15721 aagttgatac tcaggccaat aatatttacc ataattactt acgtgggatt ttctcttttt
    15781 ttgagtttac tggctaattt aatcggagtt tcaagaggag aagtgcatcc tgcaatcaaa
    15841 ttgaatcctg tgcaattcct gctttttgtg gttattgctg ccagtattgc cgaagaattg
    15901 ttgttccgcg gctttttgct taatttgctt gctccgttaa aacagagagg aatcaagatt
    15961 ttaaacagga aaataagttt atctgtgatg attagtgccc tgctctttgg cttagctcat
    16021 ttgagcctgg cttcctttgg ctacagtcct tattttttga ttcgcacagt tgttttcacc
    16081 tgctgcattg ggctgatagc cggttattac caggaaaaat acaacaacaa tgcttatgcg
    16141 ataacggtgc acatgacatc caacctggtt ggcatacttc ctgtaattat gctggctatg
    16201 caaaaagctt gaaaagaatc cttttatcaa tctcaatgca acgattcttt tgttttgcaa
    16261 gactttcttt tattcttccg gaagtattct ttttatatcc tccagagagg ggttaatggc
    16321 aattatttta cctttattgt ccatcaaaaa ctgaccgcct ccacctccgg agacacaata
    16381 tttttcccat aatttatttt catcatttat ttcggctgtc agcaaccagg gatacttgta
    16441 tttttgaacg gtctttacat attgttcttc agtactaata cctccaatca caccgacaac
    16501 tacaaatcct ttttctttta attcctcata aacgggcagt attttttgac tttttcttct
    16561 aagtatatga caatttttag aaaattatca tttaatatat tgattatcaa ttaaatatct
    16621 ttgcaaaaga ccttggaatt tcgtaacttt aaggtattaa ccatcatatt attaccatgt
    16681 tgcgaagctc caaggcccac tacaaaagta gcgaattgta tccgattctg aacatgcatt
    16741 tcggaaaaag tatgaatctg gcaagaatca aggctatgag cctgatgata tgtgccctct
    16801 gcaaggtgca gcgggtggca tataccaaac ttgcatctgc gttcgatacc gacgcaactg
    16861 ccggctcttc tttaaggaga atccaacgcc tgatagccga atgtgtgatt gatacggacc
    16921 tcatcgcaaa gctcattttg aaattaatac ccgttaagag tccctacaat cttgctatgg
    16981 acaggaccaa caggaagtac ggcaatacga acatcaacat tctcactctc ggcatcatat
    17041 atgatgggat ggcattccct gtcgtctata cgatgatgga caagaggggc aactccaaca
    17101 ctcgggaacg catcaaactg gtcaatcgtt tcaaaaggat tgcagggaaa gattccatag
    17161 ctcatctgat ggcagacagg gaattcgttg gagaagaatg tcttggatac ttgaatgctt
    17221 gcggtatcca ctatcacatc aggatacgcg agaacttcca tgtcatcaga catggtcgca
    17281 aggctaaagc cttttggctt ttcaatgatg tgaagttggg tgaaagcaag catcttgatg
    17341 gtatctacta tgtgaacgga caggcatgtt acctgtccgg ttctaaaatc aaggacaaga
    17401 aggggaaacc agagctgcag atacttgttt cgtactgcaa tgctgaaaaa gccctcgaga
    17461 tgtataagct tagatggcag atcgaaacgc tattcaaagg cctgaaatcc agcgggttca
    17521 atatagaagg ctcgcatgtc aggaatcttg atcgtatgtc gaatctgttt gctatcatta
    17581 tggttgcgta tgtctggtgt taccttgtag gaatttatat tcacgaaaac atcaagcaga
    17641 taaaggtctt gaagcacgga agaagggcgg tgagcttgtt caagtatgga ctggactaca
    17701 tatgccaatg ccttgtcaat catacaaacc gatatcgaat caatgttttc aaatttttgt
    17761 catatactta
//
LOCUS       DAOSTD010000034        16273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_3677_length_16273_cov_10.562215, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000034 DAOSTD010000000
VERSION     DAOSTD010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16273
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3677_length_16273_cov_10.562215"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..575)
                     /locus_tag="PLI37_03780"
     CDS             complement(177..575)
                     /locus_tag="PLI37_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOQ58169.1"
                     /translation="MSRFELFLKYPLFKGMSMDELFPLLTKVSLDFDNYAAGDLIFNK
                     DIVCENLVFLLDGQVELENEQNEKRIAQGPELIAYTRLFGRKRNMPLTARALDNCSIM
                     QIDRKSLLFLMRNNQTVLCNYLDLISDMIA"
     gene            656..1249
                     /locus_tag="PLI37_03785"
     CDS             656..1249
                     /locus_tag="PLI37_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOQ58170.1"
                     /translation="MIMKINLKEILSAFTVLFAIIDIIGSTPIIIDLQKKPGGVPALK
                     ISLISFLLLIGFFFLGDGVLALFGVNISSFAVAGAIVIFAVAVEMTFDIEVFRHKGPP
                     EVSSIVPLVFPLIVGAGSFTTMLSLKAEFQTINIIIALFLNMVVVFVVLKGIKRIEKL
                     IGNAGIYVARKFFGIILLAISVKLFTTNLGGLLSQFK"
     gene            complement(1325..1615)
                     /locus_tag="PLI37_03790"
     CDS             complement(1325..1615)
                     /locus_tag="PLI37_03790"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOQ58171.1"
                     /translation="MRRKNTQRLAEVLDEVLKNQQLQGKLNETAAIRLWPEVVGKALA
                     EHTDNLYFKHSVLHVHVKSAIIRNELMLIRPLLLKRLNEKIGSQAVKNIVLH"
     gene            complement(1623..2723)
                     /gene="recF"
                     /locus_tag="PLI37_03795"
     CDS             complement(1623..2723)
                     /gene="recF"
                     /locus_tag="PLI37_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852665.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOQ58172.1"
                     /translation="MYLQKLSIFNYKNIQEASIEFSPRLNCFFGNNGVGKTNFLDAIH
                     YLSFCKSHTNPIDSQNIFHEADYFVLQGNYLIEGKEEEYYCAMKRRQKKIFKFNKKEY
                     ERLADHIGRLPLVIVSPADENLIREGSDERRRFMDIALSQFDRSYMEALMSYNKALCQ
                     RNALLKNEQMNREDSLYEMYDAPMSDYAGLIYQKRREFIAAFQPVFEHYYHIVSDRSE
                     SIGLQYKSHLEQGNLQEQFLRSREKDIILGFTTKGIHKDELEMQIDGYPIKRVGSQGQ
                     NKSYLIALKLAQYEYLKQAGKKKPILLLDDLFDKLDASRVERIIQLVSKEDFGQIFIT
                     DTHREHLNMILQDNGKDFRVFHIENACICPIK"
     gene            2841..3539
                     /locus_tag="PLI37_03800"
     CDS             2841..3539
                     /locus_tag="PLI37_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ58173.1"
                     /translation="MAKKKVSKKAAPKEEMQNIGEVLTRSEQFIENNQKSLLVALLVI
                     VVVVSGIILFRNSYMGPKEKEAQEMMFRGEQYFAADSFELALHGDDYEFIGFEGIIDN
                     YGITKTAKLASAYAGLCYKHLGDYEKAIDRLNKLNAVDIMVSPALTGAIGDCYVELDQ
                     FQKALPYFERAGQSDNNLLAPIYLMKAGRVYEALGKYDSARGVYEKIKKKYPLSQEGL
                     NVDRYIERTRLQKK"
     gene            3536..4030
                     /gene="ribH"
                     /locus_tag="PLI37_03805"
     CDS             3536..4030
                     /gene="ribH"
                     /locus_tag="PLI37_03805"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOQ58174.1"
                     /translation="MSTKNLSEYNPDTLPDASDMKIGIVVSEWNYDVTVKLLEGAREF
                     LLRNNVAPENIHVAWVPGSFELIYGAARMEGSEPVDAVIALGSVIRGETPHFDYVCSG
                     VTQGIAQLNAKGSIPFIFGLLTTDDMQQALNRAGGIHGNKGVEAAKTAIQMIYFDRQL
                     DYLD"
     gene            complement(4090..5925)
                     /locus_tag="PLI37_03810"
     CDS             complement(4090..5925)
                     /locus_tag="PLI37_03810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOQ58175.1"
                     /translation="MEKYLVSARKYRPDNFKSVVGQSALTTTLQNAIKNKQLAHAYLF
                     CGPRGVGKTTCARIFAKAINCFHPNNQGEACNKCESCVSFNEQRSYNIHELDAASNNS
                     VDDIRSLTEQVRIPPQLGKYKVYIIDEVHMLSSSAFNAFLKTLEEPPAHAIFILATTE
                     KHKIIPTILSRCQIYDFHRISINDICAYLQYVADSEGIETEPEALNAIALKSDGGMRD
                     ALSIFDQVSSYGEGKITYKHVIDNLNILDYDYYFRLVEAFLSENTTESLLLFDQILNQ
                     GFDGLHFINGLASHFRDLMVSKDPRSISLLEQAESVRAKYKKQAEECSMSFLFKALKL
                     SNECSLQYNQSKNKRLLVELCLIQLCLTDEAKKKPQGLKTDNTPSPKVEPNPLPASKT
                     QTPDSPSSPSMASNPKPATPTDSASQPNPQTGNTGESDKETRPHQQAAVPEPAPQNKN
                     PNTGTDKERPLAGLTTSIRRRKIQAEENQSREATEKETIKTEEHFSEENLIEAWLRFA
                     GTLKEDPYLSNSMQACKPRLLNKSDFEVVVFHPLTAQRLEAIRADIENSLQRDLQNGK
                     IHMQLRISEENELKKPLSPQDQLDEMIKKNPNLQSLKQKLSLELQ"
     gene            6066..6365
                     /locus_tag="PLI37_03815"
     CDS             6066..6365
                     /locus_tag="PLI37_03815"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOQ58176.1"
                     /translation="MRKILAFYEKIKPYLNKYLIVLLLFGVLIVFIDEHNLIRRMRYD
                     SKIRALRKEIRQYERERDRALEQLEDLRTGSEELEKIARERYLMKKDDEEIFLLE"
     gene            6367..6711
                     /locus_tag="PLI37_03820"
     CDS             6367..6711
                     /locus_tag="PLI37_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58177.1"
                     /translation="MSKETRNNLLFTLAAVLILISSVLVMEHQAWARFSFAAGVVLYI
                     AHRALTAYKGDDFRLKRLNRMFAFNAIVLILAVYLMFKNSNSWVAILLIVAVIELYTA
                     FRSASYQKENGQ"
     gene            6713..7450
                     /locus_tag="PLI37_03825"
     CDS             6713..7450
                     /locus_tag="PLI37_03825"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOQ58178.1"
                     /translation="MSSSSFRFKQFEVFHDRCAMKVGLDGVLLGAWAKPSAELSEAAR
                     LKILDVGSGSGLIALMLAQRFPSSSVFGIEKDIQAALQAQENVVRSPWPERIHIIQGS
                     FPESINDNFSPEEQEFDLIVSNPPYHRNALKAKAYTRNLARHSDKLSFEQLAVSVAGL
                     LSEKGIFSLIVPAEAAEFIEELCWGQKLYPTSICRISHRQGKEPKRTLLAFSRSRADI
                     SRRELQIEDKKGEYTAEFKALTSDFYL"
     gene            7516..10944
                     /gene="ileS"
                     /locus_tag="PLI37_03830"
     CDS             7516..10944
                     /gene="ileS"
                     /locus_tag="PLI37_03830"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOQ58179.1"
                     /translation="MSKKFAEYTEFDLSAINKSMLEKWESTDLFQKSTALREGGPSFV
                     FYEGPPSANGMPGIHHVMARTIKDIFCRYRTMKGFEVKRKAGWDTHGLPVELGVEKAL
                     GITKEDIGKKISVIDYNAACRKDVMKYTREWEELTRKMGYWVDMSDPYITYDNRYIET
                     LWYLLKQLYQKGLLYKGYTIQPYSPAAGSGLSTHELNQPGCYRDVKDTTCVAQFKIKN
                     PRPEWSQHGEAFFLAWTTTPWTLPSNTALCVGPAISYVAVQSYNFYTGQPATYILAKD
                     LLTAHFSPKAAELPLDRYNAGEKNIPYKVLPQDYKGEDLLGIEYEQLIPWVNPGGKAF
                     EVISGDFVSTDDGTGIVHIAPTFGADDQFVAKKFSISPLLLVDKEGNTRPMVDTAGKF
                     FRLEDLDPAFVGSNINVEKYAPYAGRYVKNEYMPEGLESEDNLDVDLCVMLKQENRVF
                     KIEKQVHNYPHCWRTDKPILYYPMDSWFIKTTACRDQMMALNQTILWKPASTGTGRFG
                     KWLENLQDWNLSRSRFWGTPLPIWRTEDGSEELCIGSVAELYQEIEKSVDRGFMKANP
                     YKDFVPGLYTAENYSVEKIDLHRPYVDEIVLVSPSGKAMRREPDLIDVWFDSGAMPYA
                     QIHYPFENKEAFDSGQCYPADFIAEGVDQTRGWFFTLHALAAMLFDSVAFKSVVSNGL
                     VLDKNGNKMSKRLGNAVDPFVTIEKYGSDPLRWYMITNASPWDNLKFDEEGIEEVRRK
                     FFGTLYNTYSFFVLYANVDGFDNQAAQIPWQERPEIDRWILSQLNSLIRDTDQYYNDY
                     EATRSGRAIADFVGENLSNWYVRLNRKRFWGGGYSKDKLSAYQTLYTCLETVSRLMAP
                     IAPFYADRLFLDLNAVAKGERCESVHLADYPKCNYEAIDSALENRMQMAQQISSMVLA
                     LRRKVNTRVRQPLQTIMIPALDAAQKEAIEAVRELILNEVNVKELKFIDTAGGLLIKR
                     IKPDFKKLGPRCGKNMKQVAKMLGEMQQDAIAEFEQKGYCTIELEGKNLRIETADVEI
                     LSEDIPGWLVANEGRFTVALDVNITEELRREGLARELVNRIQNLRKAQNFEITDRIHI
                     LIEKHPAFVGTMEDWGEYISAQTLALSLQQVDRLEEGTILDMDDMSLNIKILKA"
     gene            11054..11431
                     /locus_tag="PLI37_03835"
     CDS             11054..11431
                     /locus_tag="PLI37_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOQ58180.1"
                     /translation="MSEIIRYSDAELEEFRQLIQKKLDKARNEYEQLKASINNIDGND
                     VMDTSPIFKVFEEGANTLLKEETGRLAQRQMKFIENLEAALVRIDNKTYGICRETGKL
                     IPKERLRAVPHATLSIEAKNIKS"
     gene            11435..12088
                     /locus_tag="PLI37_03840"
     CDS             11435..12088
                     /locus_tag="PLI37_03840"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOQ58181.1"
                     /translation="MAKPSKVWKAFVLIAIILVVDQAVKVYIKTHFQLHESVRVTDWF
                     YIYFTENSGMAFGWEFFDKIFLTLFRIIAAGLLIWYMVHISRKKQFPTGYVLSIALII
                     AGALGNVIDCVFYGLIFDHSYGQVAGFFPEGGGYAPIFYGKVVDMLFFPLINTTLPDW
                     LPIWGGRDFLFFRPVFNIADTSITTGILILLIFYRSCLAEDTGKKSKKNKAGSPHTA"
     gene            12100..12933
                     /locus_tag="PLI37_03845"
     CDS             12100..12933
                     /locus_tag="PLI37_03845"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOQ58182.1"
                     /translation="MNGIKDILNKVLLLGMSVLMLTACRQEERKVLSQKKMAAVLTDI
                     YLTEAVLQNVDRKKKTEWSRGLEDVFFQDLAYGRVLEKHRITEDVFYNSVAHYSQQYK
                     VYAKIYEQVEFNLQAIRDEVDRRDKLETQLRELAQQQAALIEALDTASYIAWFEIWAI
                     DTLVLSDVLGLQADSVVLSDTSVLQADSVVFSDTLGMQTDSVVLLETLVSQTGDVLLS
                     PLNDSIVYTISFWPKPLVRDSIEWTMVDFMPFMESGRLFREGKNSVTINLKRTIKEVG
                     H"
     gene            complement(12914..13681)
                     /locus_tag="PLI37_03850"
     CDS             complement(12914..13681)
                     /locus_tag="PLI37_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOQ58183.1"
                     /translation="MLSKAKIKWIHSLNLKKNRKELHLFVAEGHKLVTELLPHMSCRF
                     LTATTEWLQRYPDIRAAEIAEASLQELNKASNQQHPQEVLAVFEIPDRKPDLNYLKNS
                     LTLALDNIQDPGNLGTIVRTADWFGINTLLCSVGCADAYNPKSVQASMGSIARVAVHY
                     VKLEEFIPTLNLPVFGAFLEGKNLYEFRHLPQAAVILMGNESNGISGKLKPLVNEKLY
                     IPEFPPGSIRAESLNVSIATGIICAAFRKPPTNARLP"
     gene            13799..16135
                     /locus_tag="PLI37_03855"
     CDS             13799..16135
                     /locus_tag="PLI37_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOQ58184.1"
                     /translation="MRVTSLFYVLIPLLSISCNTLKYVPEDKQLLTGTSIEIKDEKFP
                     KKELKNYLRQQPNSRFWGLFRINLGLYNISGQDTSKWINRGLRKIGEAPVIYEELLTQ
                     RSAMALERYLFSKGYFDSEVEIRTVSKGRKIKTIYNIRPNQAYRINSYTAEVDPRDPF
                     HMLIYGALPLSAVQTGDKFDTGLLDEERSRLVSWARNYGYFAVNKDHFSFEADSSLNK
                     NLVDLRLIINPYLQRSREGGSQYVPHKQYRIRRVYFMLDVPSSAYLRNLGIQGSSHFF
                     TELADYDSIPYENYAVVYRDKPFIKPKVLVDNCMIIPGDYYDARAVNRTYSRFNSMQN
                     MKYVNIRFEEISAQDSTSGSEAQLDCYVVLTPGQNNIISMDVEGTNTAGDLGVAGTLT
                     YSHLNLLRGGETFSTSLRGAYEALSSSFQNDYTEFGGEIGLQFPDFKIPFLSSDFKKR
                     VDANTNFKISYDNLSRPEFLRTMASGAVEYIWRNQRLRQNLSLLDLAYVYMPRVDSAF
                     KATYLTDNSYLKYSYEDHFILRTQYGILYSSVPFGVSNRTFFTIRANIESAGNVLRLA
                     YSLLDAEKGENQAYTMGKIPFSQYLKGEFEYARSVVLNTRNRFAYRAGVGVAYPYGNS
                     KILPFEKRYFSGGANSVRGWSVRTLGPGTYKNNLNRIDFMNQSGDLKLDLNAELRSKL
                     FWVLEGGLFVDVGNIWTLRNYDNQPGGQFQFDEFYKQLACSAGLGLRFDFNFFLVRVD
                     AGMKVFDPSGLTADDRWRIKHIDQWNDFALHLAIGYPF"
BASE COUNT         4347 a         3396 c         3901 g         4629 t
ORIGIN      
        1 tgaagaccaa taacggtcag cgctgtcata ataaaaaata atttagtttt catctattca
       61 gttctttctg ttttgtctta attaattcct tcagcaacag tattatcgtg gctgccttga
      121 gaatcgttta taagtagtag aaaatggaga agtagaatga gtttgtcgat gccggtttaa
      181 gcaatcatgt ccgaaatcaa gtcgaggtaa ttgcataata cggtttgatt gttgcgcatt
      241 aaaaacagta gagactttct gtctatctgc atgatgctgc aattatccaa agctctggca
      301 gttagtggca tattgcgttt ccggccgaat aaacgtgtat aggcgatcaa ttcaggccct
      361 tgtgcgatgc gtttttcgtt ttgttcgttt tcgagttcta cttggccgtc cagtaaaaag
      421 accagattct cgcaaacgat gtctttgtta aatataagat ctccggcagc atagttgtcg
      481 aagtcgaggc tgactttggt taaaagcggg aacaactcat ccatactcat tcccttaaac
      541 aaagggtatt tcaaaaaaag ttcaaatctg gacatctcgc attttttaga gtataaacaa
      601 ttccttggcg aaagtactca agatttctga aaatgcgcta tttttgccaa aaataatgat
      661 tatgaaaatc aatttaaaag agatactaag cgctttcacg gttttatttg ccatcatcga
      721 tatcattggt tcaaccccga tcatcattga tttgcagaaa aagccgggag gagttcccgc
      781 tttaaaaatt tccttgattt cttttctcct tttgattggt tttttctttc tgggagacgg
      841 ggtgcttgct ttgttcggag taaatatatc ttcgttcgct gtagccggtg ctatcgtaat
      901 ttttgctgtg gcggtcgaaa tgactttcga tattgaagtg tttcgccaca aaggcccgcc
      961 cgaagtttct tccatagtgc ctttggtctt tcctctcatc gtcggtgccg gttcttttac
     1021 aaccatgctc tcgttaaagg ctgaatttca aacaattaat atcataatag cgctgttcct
     1081 gaatatggtg gttgtctttg tggtcctcaa aggaatcaag cggattgaaa agcttattgg
     1141 aaacgcgggt atttacgtcg cacggaaatt tttcgggatc attctgttgg ccatttcagt
     1201 aaaattattc acaaccaatt tgggtggcct gcttagccaa ttcaaatagc attggatttg
     1261 cacgatatgg aagatttggg cgaattagtg cttgcctagc taattcagat agaatccggg
     1321 ggaatcaatg cagcactatg tttttaacag cctggctgcc gattttttcg ttgaggcgtt
     1381 taagcaacag aggcctgatg agcatcagtt cgttacggat aatggccgat ttgacgtgga
     1441 cgtgcaaaac ggaatgtttg aaatacaagt tgtccgtatg ttcggccagg gctttaccca
     1501 ccacttccgg ccatagcctg atggctgccg tttcgttgag cttgccttga agttgctggt
     1561 ttttgagcac ttcgtccagt acttctgcca gacgttgggt gtttttccta cgcataaagc
     1621 tcttatttta taggacaaat gcaggcgttt tctatgtgaa aaacgcgaaa atctttacca
     1681 ttgtcctgta aaatcatatt caggtgctcc cggtgagtat cggtaataaa aatttgcccg
     1741 aaatcttctt tggaaaccag ttgaatgatg cgttccaccc gcgaagcgtc cagtttatcg
     1801 aaaagatcgt cgagcagaag tatgggtttt ttcttgccgg cttgtttcaa gtattcgtat
     1861 tgtgccagtt ttaaggcaat caggtaactt ttgttttgtc cctgggagcc cacccttttg
     1921 atgggatagc cgtcgatttg catttcgagc tcgtctttgt ggatgccctt ggtggtaaaa
     1981 cccagaataa tatctttttc cctcgatcgt aggaactgtt cctgcaaatt accctgttca
     2041 agatgcgatt tatattgcag tcctatactt tcgctgcggt cggaaacgat atgataataa
     2101 tgttcaaaaa ccggctgaaa ggcagctatg aattccctgc gtttttgata aatcaacccg
     2161 gcatagtcgc tcatgggagc atcgtacatt tcgtagagag aatcctctct gttcatctgt
     2221 tcgtttttca acagagcgtt gcgttggcaa agagccttgt tgtaactcat gagggcctcc
     2281 atgtaggagc gatcgaactg cgaaagggca atgtccataa acctccggcg ttcgtcactg
     2341 ccttcgcgaa tcagattttc gtcggcggga gacacgatga ccaggggtaa gcgacctatg
     2401 tggtcggcca ggcgttcgta ttctttttta ttgaatttga atattttttt ttggcggcgc
     2461 ttcatggcgc aataatattc ttcttccttg ccctcgatca ggtaattgcc ctgaagcaca
     2521 aaatagtcgg cttcgtgaaa aatattctga gagtcgatcg gattggtgtg gcttttacaa
     2581 aacgaaagat aatgaatggc atccaggaaa ttggttttgc ccactccgtt attaccgaaa
     2641 aaacaattta atcggggaga aaattcaata ctggcctctt gtatgttctt gtaattgaaa
     2701 atggatagct tttgcaggta catgggtgag tttcggacaa aatgcctaca aaagtataaa
     2761 aaaaatcacg gaaattttat taatagaacg aaatgctata cttttgcaca ccaaatttgt
     2821 cttatacaat tacaaaagaa atggctaaga aaaaagtatc caaaaaggca gctccgaaag
     2881 aggaaatgca aaatattggc gaagttttga cccgttcgga acaattcata gaaaacaacc
     2941 agaaaagtct tttggtagct ctgttggtta tcgttgtggt ggtaagtggt atcatacttt
     3001 tcagaaacag ttatatgggt cctaaagaaa aagaagccca ggaaatgatg ttccgagggg
     3061 aacagtactt tgccgcggat tcatttgagc tggctctgca tggagacgat tatgaattta
     3121 taggttttga aggtatcatc gataattacg gcattaccaa aacggccaag ctggcctcgg
     3181 cctatgcggg tttgtgctac aagcacctgg gcgattacga aaaagccatc gacaggctca
     3241 ataaactcaa tgcagtcgat attatggttt cgcctgcgtt gacgggagca atcggcgatt
     3301 gttatgtgga actcgatcaa tttcaaaaag ccttgcctta tttcgagaga gcaggccaat
     3361 ccgacaataa tttattggct cccatttatt tgatgaaagc agggcgtgtt tacgaagcac
     3421 tgggaaaata cgacagcgcc cgcggagttt atgaaaaaat aaaaaagaaa tatcctcttt
     3481 cgcaagaagg tctgaatgta gacaggtaca tcgaaaggac aagattgcag aaaaaatgag
     3541 tactaaaaat ctttccgaat acaaccctga tactctgccc gatgcgtccg acatgaaaat
     3601 aggcattgtg gtttccgaat ggaattacga tgttacggtt aaattgctgg aaggggcgcg
     3661 tgaatttttg ctgcgtaaca atgtggctcc tgaaaacatt cacgttgcgt gggttccggg
     3721 cagttttgag ttgatttacg gggcagccag gatggaaggt tctgaacccg tggatgctgt
     3781 tatagctttg ggtagtgtga tccgcggaga aacgcctcac ttcgactatg tttgttccgg
     3841 agtaacgcag ggtatcgccc agctgaatgc taaagggagc atccccttta ttttcggtct
     3901 gctcaccacc gacgatatgc agcaagcttt aaacagggcc ggcggcatac acggcaataa
     3961 aggggtggaa gctgctaaga ccgccataca gatgatttac ttcgaccgac agctggatta
     4021 tttggactaa agaagtttgc cgcataatcc cggcacattt cctcaaactt cagttggaag
     4081 aaaagccgtt tattgcaact caaggcttaa tttttgtttc aaactttgca ggttcggatt
     4141 ctttttgatc atttcgtcca gctgatcctg agggcttaag ggctttttta attcattctc
     4201 ttcgctgatt cgtaactgca tgtgaatttt gccgttttgc aaatcccgtt gcaaactgtt
     4261 ttctatatct gcccgtatgg cttcaagccg ttgagcggtc agcggatgaa aaaccactac
     4321 ttcgaaatcg ctcttgttca acaaacgagg tttgcatgcc tgcatggagt tcgataaata
     4381 aggatcttcc ttgagcgtgc ccgcaaaacg gagccaggct tcaatcaggt tttcttcgct
     4441 gaaatgctct tcagtcttaa tggtttcttt ttcagttgct tcccggctct gattttcttc
     4501 tgcttgtatt ttacgtcggc gtatggacgt ggtcagaccc gctagcggcc tttccttgtc
     4561 tgtcccggtg ttgggatttt tattttgtgg ggcaggttcg ggtactgcgg cttgctgatg
     4621 tggccgggtc tccttgtcgc tctcccctgt gttgccggtc tgaggattag gctgagatgc
     4681 agaatcggtc ggtgttgccg gctttggatt ggacgccatg gatggagaag agggactgtc
     4741 cggagtttgg gttttagaag caggaagtgg atttggctcg actttgggcg aaggggtatt
     4801 atccgttttc aagccttgcg gttttttttt tgcttcgtcg gtcagacaaa gttgaatcag
     4861 gcaaagctcc accaaaaggc gcttgttttt gctttgatta tattgcagac tgcattcgtt
     4921 gctgagtttg agggctttga acaaaaagct catgctgcat tcttcggcct gttttttata
     4981 tttggcgcgt acactctcag cctgctccaa caggcttatg ctgcggggat cttttgaaac
     5041 catgagatct ctgaaatgag aagccaggcc gttgatgaaa tgcaagccgt caaaaccctg
     5101 gttcaaaatc tgatcaaaca acaacaaaga ctctgtggta ttttcgctca aaaaagcctc
     5161 aaccagcctg aaataatagt cgtaatccag aatgttcaga ttatcgatga catgcttata
     5221 cgtaattttg ccctccccgt aactggaaac ctggtcgaaa atggagaggg catcgcgcat
     5281 gcccccgtcc gatttcagag caatggcgtt cagggcttcc ggttccgtct cgataccctc
     5341 ggagtcggct acgtactgca gataggcaca gatgtcgttg atgctgatgc ggtgaaaatc
     5401 ataaatttga caacgggaga gtatggtagg tatgatttta tgcttttcgg tagttgcgag
     5461 aataaaaata gcgtgagcgg gaggctcttc cagtgttttg agaaaagcat taaaagcgct
     5521 ggaagagagc atgtgtactt cgtcgataat gtaaaccttg tatttgccca gttgtggtgg
     5581 aatgcgcact tgttcggtca gagaacggat gtcgtcaacc gagttgttgg aggcggcatc
     5641 cagttcgtga atattgtagg aacgctgctc attgaaagaa acgcaagatt cgcatttgtt
     5701 acaggcttct ccctgattgt tgggatgaaa acaattgatg gctttggcaa aaatcctggc
     5761 acaggtggtt ttgcctactc ctctggggcc gcaaaacaaa taggcatgag ccagttgctt
     5821 gttcttgatg gcattttgga gagttgtggt aagggcactc tgcccaacga ccgatttaaa
     5881 gttgtccgga cggtatttcc gtgctgaaac caaatatttt tccatacgaa ttacgctgct
     5941 ggcttaggtg gacaaagata cataaggctt ttctaaaaag gaagttccgg caacgatttt
     6001 tgatttagaa aacacttggg catccggatt attctcgtaa ttttgttctg ttcataaatc
     6061 cgcctatgcg caagattttg gcgttttacg aaaagattaa accgtatttg aataaatacc
     6121 tgatcgtgtt gcttttgttc ggtgtgctga ttgtgttcat cgatgaacat aatctgatca
     6181 gacgtatgcg atacgacagt aaaatacgcg ccttgcgtaa agaaatacga caatacgaaa
     6241 gagaaagaga ccgggctctg gaacagcttg aggatttgag aaccggcagt gaagaactgg
     6301 agaaaattgc ccgagaacgc tatttgatga aaaaagacga tgaagaaatt tttttgctgg
     6361 aatagaatgt ccaaagaaac ccgtaataac ctgctgttta cattggccgc ggtgttgatc
     6421 ctgatttcat ccgttttggt aatggaacat caggcctggg ccaggtttag ttttgcagcc
     6481 ggagtagtcc tctacattgc gcatcgtgct ctcacggcct acaaaggcga cgatttcaga
     6541 ctaaaacgac ttaacagaat gtttgccttc aatgccattg tccttatact tgctgtctac
     6601 ctgatgttca aaaacagcaa ttcctgggtg gcgatcttgc tcatagtggc ggtaatcgaa
     6661 ttgtatacgg cttttcgttc agcttcctat cagaaagaaa acggacaata gcatgtcatc
     6721 ctcctctttc cgttttaagc aatttgaggt ttttcacgac cgttgcgcca tgaaggtggg
     6781 gctggacggg gtgttgttgg gtgcatgggc aaaaccctcc gccgagttgt ccgaagcggc
     6841 aagattgaag atactcgatg tgggcagcgg ttccggcttg attgcgctga tgttggctca
     6901 gcgttttccc tcctcttcgg tttttggtat cgaaaaagac atacaggcgg cattgcaggc
     6961 acaggaaaat gttgtccgca gcccctggcc cgaaagaata catattattc agggctcttt
     7021 tcccgaaagc atcaacgaca atttcagtcc cgaagagcaa gaattcgatc tgatcgtttc
     7081 caatcctcct taccatagaa atgccctgaa ggccaaagct tacacgcgta atttggcccg
     7141 acacagcgac aagttgagtt ttgaacaatt ggctgtaagc gttgccggct tgttgagcga
     7201 gaaaggaatt ttttctctta ttgtgcctgc cgaagccgca gagtttatag aagaactctg
     7261 ttgggggcag aagctttatc ctacaagcat ttgccggatt tcacaccgtc agggtaaaga
     7321 acccaagcgt accctgttgg ctttttccag gtcgcgggcg gatatttctc gtcgggaatt
     7381 gcaaattgag gacaagaaag gtgaatatac tgctgaattc aaggccttaa cctcggattt
     7441 ttacctttaa atcctttttc atagtacatt tttttaatgt acctttgcgc cctcaattag
     7501 gataacgatc gcaagatgtc aaaaaaattt gctgagtaca cagagttcga tttgtcggcc
     7561 atcaacaaat cgatgttaga gaaatgggaa tcaaccgatc tttttcagaa aagtacggct
     7621 ttgagagaag gagggccgtc ttttgtgttt tacgaaggac ctccttcggc caatggcatg
     7681 cccggaatac accatgtaat ggccaggacc atcaaggata tattttgccg ttacagaacc
     7741 atgaaaggct ttgaggttaa acgcaaagcc ggttgggata cccacggcct ccctgtggaa
     7801 ttgggagtgg aaaaagccct gggaattacc aaagaagata taggcaaaaa aatttccgtc
     7861 atcgactaca atgccgcttg tcgtaaggat gtgatgaaat acacccgcga gtgggaggag
     7921 cttacccgca agatgggtta ttgggttgat atgtccgatc cttacatcac ctacgacaat
     7981 cgctacatcg aaactttgtg gtacttgctt aagcaattat accaaaaagg tttgctttac
     8041 aagggttata ccattcagcc ttattctccc gccgccggtt cgggcctgag tacccacgag
     8101 ttgaatcagc ccggatgcta cagggacgtc aaagacacaa cctgtgtggc ccagttcaag
     8161 ataaaaaacc cacgtccgga gtggagccaa cacggagaag cctttttcct ggcttggacc
     8221 accactccct ggactttacc ctccaatacg gctctttgcg tggggccggc gatcagctat
     8281 gtggctgtgc aaagctataa tttttacacc gggcagccgg ccacctatat cctggccaaa
     8341 gatttgctga ctgcccattt cagtccaaaa gcagccgaat tgcctttgga tcgttacaat
     8401 gcgggggaaa agaacatacc ttacaaagtg ctgccacagg attacaaagg agaggatttg
     8461 ctgggcatag aatacgagca attaatcccc tgggtaaatc cgggagggaa ggcttttgag
     8521 gtaatcagcg gtgactttgt ctccaccgac gacggaacgg gtattgtaca cattgccccc
     8581 acttttggag cagacgacca atttgttgcc aaaaaattca gcataagccc tcttttattg
     8641 gtcgataaag aaggcaatac gcgccccatg gtagacaccg ccggcaaatt cttcagattg
     8701 gaagatctcg atccggcctt tgtcgggagc aatattaatg tggaaaaata cgcaccatac
     8761 gcgggccgtt acgtcaagaa cgaatatatg ccggaaggtt tggaaagcga ggataatcta
     8821 gatgtggacc tttgtgtgat gctcaaacag gagaaccgtg tttttaaaat tgaaaagcag
     8881 gtacacaatt atccccattg ttggcgcacc gataagccaa tcctgtatta tcccatggac
     8941 agttggttta taaaaacgac cgcctgccgc gaccaaatga tggctctcaa tcagactatt
     9001 ttgtggaaac cggcctccac cggtaccggc cgctttggca agtggcttga gaatctgcag
     9061 gactggaacc tgtcgcgcag tcgcttctgg ggtacacctc tgcccatctg gcgtaccgaa
     9121 gacggtagcg aagaactctg tatcggctcg gtagctgagc tgtaccaaga gatagagaag
     9181 tctgtcgacc gcgggtttat gaaagcaaac ccttacaagg attttgttcc cggtttgtat
     9241 acagccgaaa attattcggt cgaaaaaata gacctgcatc gtccctatgt ggacgagata
     9301 gtgttggttt ctccctcggg caaagccatg aggcgtgagc ctgacctgat tgacgtctgg
     9361 ttcgattccg gagctatgcc ttacgcccag atacattatc ccttcgaaaa caaagaagcc
     9421 ttcgactcgg gtcaatgtta tcctgccgat tttattgccg agggagtaga ccagacccgc
     9481 ggctggtttt ttaccctgca cgctctggcc gccatgctct ttgattccgt tgcctttaaa
     9541 agtgtggttt ccaatggctt ggttcttgat aaaaacggaa acaaaatgtc aaaacgtctg
     9601 ggcaacgctg tagatccttt cgttaccatc gaaaaatacg gttccgaccc tttgcgttgg
     9661 tatatgatta ccaatgcctc tccctgggat aatttaaaat tcgatgaaga aggaatcgaa
     9721 gaagtcagga gaaaattttt cggcactctc tacaacacgt attctttctt tgttttatat
     9781 gccaatgtag atggtttcga caatcaggca gcacaaattc catggcagga aagacccgaa
     9841 atagatcgct ggatactttc acagttgaac agcctgattc gtgatacaga tcagtactat
     9901 aacgactacg aagccaccag atctggacgc gcaatagccg acttcgtggg tgaaaattta
     9961 agtaactggt atgtccgcct caaccgcaag cgtttctggg ggggtgggta ttccaaagac
    10021 aaactttcgg cttaccagac cttgtacacc tgtctggaaa ccgtatcccg gctgatggct
    10081 cccattgctc ctttttacgc agaccggctg tttttggatc ttaatgccgt tgcaaaggga
    10141 gagagatgtg aatctgttca cctggccgat tatccgaaat gcaattacga agccatagac
    10201 agcgccctcg agaaccggat gcagatggcg cagcaaattt cgtccatggt gctggctctg
    10261 agacgtaagg taaataccag ggtaaggcaa cccctgcaaa ccattatgat tcctgctttg
    10321 gacgccgccc agaaagaagc catcgaagct gtccgtgaac ttattctaaa cgaagtaaac
    10381 gtcaaagaac tgaagtttat agatactgcc ggcggattgc tcattaagcg catcaaaccc
    10441 gatttcaaga aactggggcc cagatgcggg aaaaacatga aacaggtggc aaagatgttg
    10501 ggagaaatgc aacaagacgc cattgccgaa tttgaacaaa agggttattg cacgatagaa
    10561 ctcgaaggca agaacctgcg tatcgagacc gccgatgtgg aaattctctc ggaagacatc
    10621 cccggttggc tggtagccaa cgaaggacgg tttaccgtgg ctctcgatgt caatatcact
    10681 gaggaattga gaagggaagg tttggctcgt gaattggtga atcgtattca gaacttgcgc
    10741 aaggctcaaa attttgagat aaccgaccgt attcatatcc ttatagaaaa gcatcccgct
    10801 tttgtcggaa ccatggaaga ttggggagaa tacatctctg ctcagacatt ggctttaagt
    10861 ctgcaacaag tggatcggct tgaggaagga acgatcttgg acatggatga tatgtcgtta
    10921 aatatcaaga ttttaaaagc ttgattattt ttaagggcat ggggagcgat ttcctattgc
    10981 tgtcgatatt tatttttgct atattcgcag attaaaaatc aaacaataga ttaaccatat
    11041 aacacaaagt accatgtcag aaattattcg ttattcagat gcagaactgg aagagtttcg
    11101 tcagttaatt cagaaaaaat tagacaaggc cagaaatgag tatgaacaat taaaagcgag
    11161 catcaacaac atagatggta acgacgtaat ggacacttcc cccattttca aagtttttga
    11221 agaaggtgcc aatactttat tgaaagaaga aaccggacgt ctggcccaac gccagatgaa
    11281 gttcatcgaa aatttggagg cagccttggt tcgtatagat aacaagacct acggcatttg
    11341 cagggaaaca gggaaactca ttcccaaaga gcgcttacgt gcagtccccc acgcaacgct
    11401 gagtattgaa gctaaaaaca ttaagtctta aaggatggcc aaaccttcca aagtatggaa
    11461 agcgtttgtt ctaattgcca tcatattggt tgtagatcaa gcggttaagg tatatataaa
    11521 aacacatttc cagttgcacg aaagtgttcg tgtgacggat tggttttata tttactttac
    11581 cgaaaacagc gggatggcct ttggctggga attttttgac aaaatattcc tgaccttgtt
    11641 tcgaataatt gcggccggct tgctgatatg gtatatggta cacataagcc gcaaaaaaca
    11701 attccctaca ggctatgttt tgagtatagc acttattata gcgggtgcct tgggcaacgt
    11761 gattgattgc gtgttttatg gtttgatttt tgatcacagt tatggccagg tagccggatt
    11821 tttccccgag ggcggtggtt atgctccgat tttttacggc aaagtggttg atatgctgtt
    11881 tttcccgttg ataaacacca ctttgcccga ttggctgccc atttggggag gccgtgattt
    11941 tttgtttttc aggcctgttt tcaatattgc cgatacctcg attactacag gtattctgat
    12001 tctgttgatc ttttaccgct catgtctcgc cgaggataca ggaaagaaaa gcaagaaaaa
    12061 taaggcaggc tctcctcata cggcctgacc accctgttta tgaatggcat taaagacata
    12121 ctgaacaaag tattacttct tggaatgtcg gttctgatgc tgacggcttg cagacaagaa
    12181 gagcgcaagg tcctttcaca aaaaaagatg gctgccgtgc tgacggatat ttatcttacg
    12241 gaagccgttt tgcagaatgt agataggaag aaaaagacag aatggtcccg ggggcttgag
    12301 gatgtttttt ttcaggatct ggcctatgga agggtattag agaagcaccg tatcaccgag
    12361 gatgtttttt ataattctgt ggcgcattac agccagcagt acaaggtcta tgcaaagata
    12421 tacgaacagg ttgaattcaa tttgcaagcc atccgcgatg aggtagaccg gcgcgacaag
    12481 ttagagacac aattacgaga gttggcccaa caacaggcag ctcttattga agctctcgac
    12541 acggcctctt acatcgcttg gtttgagatt tgggcgatag acacgcttgt tctgtcagac
    12601 gtcttgggct tgcaagcaga ctcggtggtt ttatcggaca cctcggtctt gcaagcagac
    12661 tcggttgtct tttcagatac cttgggcatg caaacagact cggttgtttt attggaaacc
    12721 ttggtttcac aaacaggcga tgtactttta tcccccttga acgattcaat tgtatatacc
    12781 atctcttttt ggccgaaacc gcttgtccgg gacagcatcg agtggacaat ggtcgatttt
    12841 atgcccttta tggaatcggg gagattattt cgggagggta aaaactctgt tacgataaat
    12901 ttgaaaagaa cgattaagga agtcgggcat tagtcggcgg tttcctgaaa gcggcacaaa
    12961 tgattcctgt agcaatggag acattgagcg attctgccct tatgctgccc ggcggaaatt
    13021 ccgggatata cagcttttcg tttacaagag gcttaagttt cccggaaatc ccattgcttt
    13081 cgttccccat caatatcacg gctgcctgtg gcagatgccg gaactcataa agattttttc
    13141 cttcgaggaa ggccccgaaa acaggaaggt tcaatgtggg gataaattct tcgagtttaa
    13201 cataatgaac cgccaccctg gcaatggagc ccatgctggc ctgtacggat ttgggattat
    13261 aagcatctgc gcatcctacg gaacaaagca gcgtattgat gccgaaccag tcggctgtac
    13321 gaacgatggt gcccaggttg ccggggtcct gaatattgtc gagcgcgagc gttaagctgt
    13381 tctttaaata attcaaatcc ggttttctat cgggtatttc aaaaacggcg agtacttctt
    13441 gagggtgttg ttgattgctc gctttgttga gttcctgcaa agaggcttcc gctatttcag
    13501 cagccctaat gtccggatat ctttgcagcc attctgtggt agccgtcaaa aaacggcagg
    13561 acatatgggg gagcagctct gttaccaatt tatggccttc ggctacgaac aaatggagct
    13621 cttttctgtt ttttttaaga ttcagcgaat gtatccactt tattttggct ttactcagca
    13681 taagcttttt ttaggaaaac aaaactacaa aagaaattca gcccgcagaa atttatctat
    13741 gtgaatttgt gaacattgct tggaatgatg tacatttgcc gctataagaa aatccggaat
    13801 gcgagtaaca agtctgtttt atgtgctgat tcccctgttg tctatttcct gcaatactct
    13861 caaatatgtg ccggaagaca aacaattgct tacgggaaca agcatagaga ttaaggacga
    13921 aaagtttccc aaaaaagagt tgaagaatta cctgcggcaa caaccaaact cgcgcttctg
    13981 gggtttgttc agaattaatc tgggattgta taatatatcc ggtcaggaca caagcaaatg
    14041 gatcaatcgt ggcctgagaa agataggaga agctccggtg atttacgaag aattgctcac
    14101 tcagcgttct gcaatggcct tggagcgcta tttgttcagc aaaggttatt ttgactcgga
    14161 agtagaaatc cggacagtga gcaaaggacg gaaaatcaaa accatttaca acattcgtcc
    14221 caatcaggct taccgaatca acagttacac ggcagaagtc gatccccgtg atccttttca
    14281 catgcttatt tacggcgctt tgcctttgtc tgcggtgcaa acaggagaca agttcgatac
    14341 cggcctgctc gacgaagagc gttcacgact ggtttcctgg gccagaaact atggctattt
    14401 tgctgttaat aaagatcatt ttagttttga ggcagacagc agcctcaaca agaaccttgt
    14461 tgatctgagg ctgataatca atccttattt gcagcgtagc cgggagggtg gttctcagta
    14521 cgtgcctcat aagcaatacc gtattcgccg ggtttatttt atgttggatg tgcccagttc
    14581 ggcttatctg cgaaatttag ggatccaggg atcttcccat ttttttacgg agctggcgga
    14641 ttacgattcc ataccctacg agaattatgc cgttgtatat agggataagc cttttattaa
    14701 acccaaagtg ttggtagata attgtatgat tattcccggc gattattacg atgcacgagc
    14761 tgtgaacaga acttattccc gcttcaactc catgcagaac atgaaatacg tcaatattcg
    14821 atttgaagaa attagcgcac aggattccac ttcgggcagt gaggctcaac tggattgtta
    14881 tgtggtgttg acacccgggc agaacaatat aatctctatg gatgtggagg gaaccaacac
    14941 ggcaggcgat ctgggagttg cggggacatt aacctattcg caccttaatt tgcttagagg
    15001 cggagaaact ttcagcacaa gtttacgtgg agcctatgag gctttgtcga gttcctttca
    15061 gaacgactat accgagtttg ggggcgaaat cggtttgcag tttccggatt ttaaaatccc
    15121 ttttttgtcg agtgatttca aaaagagagt ggacgccaat actaatttta aaatcagcta
    15181 cgataacctg tcccgtccgg aatttttaag aactatggct tccggtgccg tcgaatatat
    15241 ctggaggaat caacgtttgc gtcagaattt gagtttgctc gatctggcct acgtctatat
    15301 gccccgggtg gactctgcct tcaaagcgac ctatctcacc gataattcat acctaaaata
    15361 cagttatgaa gatcatttta tattgcgaac ccaatatgga attctttatt ccagcgtccc
    15421 tttcggggtc tctaaccgga ctttctttac catacgtgcc aacattgaat cggcaggtaa
    15481 tgtgttaaga ctggcttatt ccctgttgga tgcagaaaag ggcgaaaatc aggcttatac
    15541 gatgggaaaa atcccttttt cgcaatacct gaaaggagaa tttgaatatg cgagaagcgt
    15601 ggttctgaat actcgtaacc gttttgctta tcgtgcgggt gtgggggtgg cttatcctta
    15661 tggcaattca aagatcttgc cgtttgaaaa acgctatttt tccggaggag ccaactcagt
    15721 acgcggttgg tctgtcagaa ctctggggcc gggaacctat aaaaacaacc ttaacagaat
    15781 agatttcatg aatcagtccg gcgaccttaa gctggacctt aatgcggaat tgcgcagtaa
    15841 attattttgg gtactcgaag gcggattgtt tgtggatgtt ggcaatatct ggactttacg
    15901 taattacgac aatcaaccgg gtggtcagtt ccaattcgat gagttttata aacaattggc
    15961 ttgcagtgcg ggtctgggtt tgcgcttcga ttttaatttc tttttggttc gtgtggatgc
    16021 gggtatgaaa gtgtttgatc cctcggggct tactgcagat gatcggtgga ggattaagca
    16081 tatcgatcaa tggaacgact ttgctctgca cctggccata ggctatcctt tttgaggctt
    16141 tatataaaaa agctgtgccc ccggggagac acagcttttt tttaaatcgt actaaatggt
    16201 gtatgttaag atgttatgcc aatttaacat ttactgcatt taatcctttt ctgccttctt
    16261 ccagttcgaa agt
//
LOCUS       DAOSTD010000035        15614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_3879_length_15614_cov_17.197506, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000035 DAOSTD010000000
VERSION     DAOSTD010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15614
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3879_length_15614_cov_17.197506"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PLI37_03860"
     CDS             <1..833
                     /locus_tag="PLI37_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOQ58185.1"
                     /translation="EILGMNIFNSVFVDCTASPEISALYADLLSHNVSVVAANKIAAS
                     SDYDNYARLKELARRKGVKYLFETNVGAGLPIINTMNSLIHSGDRILKMEAVLSGTLN
                     FIFNTISAENPLSKTIKMAQEKRFSEPDPRIDLSGTDVIRKLVILAREAGYRVEQEDV
                     VKNLFIPDKYFKGSLEDFWKNIPEMDADFEAKRKLLEKDSLRWRFVAKMENGACSVGL
                     HEVGPSHPFYDLEGSNNIILIQTERYFQDPMVIKGYGAGAGVTAAGVFGDIISIANIR
                     "
     gene            835..2148
                     /locus_tag="PLI37_03865"
     CDS             835..2148
                     /locus_tag="PLI37_03865"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOQ58186.1"
                     /translation="MKYLIVLGDGMADEAMETLGGKSPMQVAFTPCMDRLAREGRCGR
                     LITVPEGFPPGSEIANLSLLGYDVSKQFEGRGSLEAASMGIDVPEGYMAARCNLICIQ
                     DGKIKNHSAGHISTSEAGELIDFLNKELGSEDLVFHTGVSYRHLLLLKGGNKELDCTP
                     PHDVSGTAFAEVLPRAVHPRAEQTARLLRELILRSQEILPAHPVNLARKARGLDTANS
                     IWPWSPGYRPAMLPLREKYPIRSGSVISAVDLIKGTGVYAGLEPIEVPGATGLYDTNY
                     EGKAQAAIDELKKKDFVFLHVEASDEASHEGNVDLKIRTIEYLDQRLIRPILEEISTW
                     DEAVTIAVLPDHPTYCRTKTHAAEAVPFLIYRSDAGSAQARPTKGPGSFFSPGTGILE
                     PSGKLFVPDAVQVFDEFSVREGFYGTLQGEEFIRAFFGLSTKADP"
     gene            2215..3531
                     /gene="thrC"
                     /locus_tag="PLI37_03870"
     CDS             2215..3531
                     /gene="thrC"
                     /locus_tag="PLI37_03870"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOQ58187.1"
                     /translation="MNYYSTNKQAPPVSLQEAVVRGLAPDRGLYMPERINTLPASFFE
                     ELPGLSFREMSYRVAMAFFGEDLPEDTLRELVYDTLSFETPLVRVDEHIYSLELFHGP
                     TLAFKDVGARFMARLLAYFIRRQGQEKVHVLVATSGDTGSAVANGFLGVEGIQVHVLY
                     PKGLVSEIQEKQFTTLGQNITALEIEGNFDDCQRLVKQAFMDTELNRRFHLTSANSIN
                     VARFLPQAFYYFNAYAQWKRYNDPSKLVFCVPSGNFGNITAGFFAWRMGLPVERFIAS
                     NNRNDVFYEYLQSGHYRPRASVATLANAMDVGDPSNFARILDLYGHSHEAITAEIAGA
                     RYDDQEIRDTVYRTFKEKNYLLDPHGACGYRALEEGLKPGESGIFLETAHPAKFKDTV
                     EDILGEAIVVPKRLQAFMQAKKQSIPLSPAFDDFKAYLMQQKGFNK"
     gene            3568..5592
                     /locus_tag="PLI37_03875"
     CDS             3568..5592
                     /locus_tag="PLI37_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOQ58188.1"
                     /translation="MEQSSMYRPETSSQGLREFTLRALLIGLVLAVVLGAANAYLGLK
                     AGMTIAATYPAAVISMAVLRFFKGSLLEENFARTVGSIGESVAAGAIFTLPAFFIAGV
                     WGGEEFSSWSSYMTASLILVTGGVLGVLFVALLRRVMVEDKELPFPESTAAAEIHKSG
                     RSSTGDSKYLVWAMVGGALVTLGSALKLFASSAKAFFKVAIAKLTSGHTIEGGFYAAA
                     PNVSPAFLGVGYIIGPRLASLNFSGSVMAWGLMVPLLLAVFGRDFVNGFAVDGVAPNS
                     QEAWMLAANTVWQSVVRIVAIGGMLLAAIFTLYRMRSSLTTGLKRSVKDLRKAASESG
                     EEVVRTEKDLKTSWVLAGIVLAAIATFCITYFIFKTTVLVAAVAATIMIVLAFFFSAV
                     SGYLVGLIGSSNNPISGLTITALVITALILVLCGVDENTGGVAAVLGVAAIVCVAAAV
                     AGEMFQDLKAGHILGGTPWKMQLGDIIGVVLSGFVMFGVLIILNNADIAMGGMQGYAG
                     GFGSEALPAPQASLMAILSNAVVAGSMTWILIIAGMLLCIALILMGVKSPMLVFVGMY
                     LPFQTVAAIFVGGLIKGLLDMRTAKMNYSPVQLAKVENTGILLASGMIAGEALMGLII
                     AIFALFNVFFSEMLPIEQPSYWIGLVLMFAMAYLMIVLPLKKAAKTNGQK"
     gene            5579..5935
                     /locus_tag="PLI37_03880"
     CDS             5579..5935
                     /locus_tag="PLI37_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HOQ58189.1"
                     /translation="MAKNKPQNLFDLIPSVNERIRIETGEDKLLQLVFPRFRSAWLNR
                     RLTPKGRSPYIHIKLEEHGSAVVQLIDGQRSVREIALALAEHFEQEPGYEHRIAVFIE
                     HLRRDNYISLRQPEVL"
     gene            complement(6016..>8055)
                     /locus_tag="PLI37_03885"
     CDS             complement(6016..>8055)
                     /locus_tag="PLI37_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOQ58190.1"
                     /translation="HCDNRDKKTFRSRYVLPLLQNPYIRKCSPKAAALEKELRIKNRL
                     YPGYDELQQDDLLSLLFSPVKTAYELGELLLKLFSLLSQTIDNDDETNTETGQTDNKT
                     NEIYLPLFQEALFKSYTQVQRLNALLKKDLLEINLQTYKNLLLQVLDLSVPFSGEPLN
                     GLQIMGMLETRCLDFKNILMLSVNEGQMPKNRAENSFIPYNLRRAFNLSGPEHQDAIS
                     AYYFFRALQRAENITLVYNSSTEGLNRGEMSRFMLQLAIESKLPIEILHLSSEIKTLP
                     GSSILVPKTPALMQQLYDMYDQAAANTNSQKRYLSPSMLNTYLDCRLKYYFQYLRGLK
                     KEEEISEQMDPLCFGTIFHAAAQYLYVKLILQKNGRKHSHENILASMEDIRAFNLALK
                     APDSPAGFLNRNAATGERLNEKIETDKQFEERIDTGEQLNGRIEAADLQALYADKDCL
                     ERAVDYFMAKEFFRLKDGQNLPALNGEQLIQRRLLIQFLKFLLKLDQNYAPFELLGLE
                     TFVEKHMELLPTPGRKLKLRVGGIIDRMDKKDETIRICDYKTGGKAESASFDKGLFKS
                     DQKRNKHVFQTFLYAGIVKDQYPASMVKPELVYIHQAGKPDYSSDIVVKKSGKSGAKT
                     NGSNVVNDFGEFKETFETELQNVVGELFDPAESFDQTSVLETCEHCDFNTICRRK"
     assembly_gap    8056..8155
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8156..9033)
                     /locus_tag="PLI37_03890"
     CDS             complement(<8156..9033)
                     /locus_tag="PLI37_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOQ58191.1"
                     /translation="MKTFLQYCAEDIYNKYADSLNELCIVFPNNRARLFFVEALYKQA
                     GKAIWAPEFSSISELFGLRCPLELGDPILLNSLLFKVYQKQGLDKESFDDFYPWGNLL
                     LKDFDNIDKNLADAGQLFRNISELAAYSDDFEYLSPEQIAAIQRFFAHFETDRSELKK
                     RFIRLWNELFNIYTQFKEELTARNIAYEGMLQRWVVEHFDDKQAFEANSYLFVGFNAL
                     NGCEKFLFGKLQKAGKARFYWDYDRYYLNNPGHEAGHFIRENLKLFPNELNESCFDNW
                     KQTAKKIRFIDSSTEHA"
     gene            complement(9030..>10053)
                     /locus_tag="PLI37_03895"
     CDS             complement(9030..>10053)
                     /locus_tag="PLI37_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOQ58192.1"
                     /translation="LQPFAALPLIPVNFQQLMNVTIFKEDYREECVQQFVDNLNLLYV
                     AFTRAEKNLIICSKAPGQGKKNTEKNQEIKSVAELIYKILSDPAHPLFSPHFKVENDE
                     EDESILEGGGRRKEEEIADTTDDPVENTQFAGFEYGKICTDSSGNEEVRTEQDLAIEY
                     RSFPRQTGFRQSNPSRDFVSEGGKGEFHNSYIDRGKLLHKLFSRIKKQSDAPAAVRSL
                     LNEGLIDEEEQDKLLQYTQTALEHPRAKEWYSGNYRLYNECVILYQDAQHKLREKRPD
                     RVIRQEDKMVVIDFKFGKALPKYRRQVARYMELLRRMGHKQVEGYLWYVDEQQVEQVE
                     AGEVEL"
     assembly_gap    10054..10153
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10154..12219)
                     /locus_tag="PLI37_03900"
     CDS             complement(<10154..12219)
                     /locus_tag="PLI37_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOQ58193.1"
                     /translation="MARINIYKASAGSGKTWRLSVEYIKLLIKNPESYRHILAVTFTN
                     KATTEMKERILHYLQLLSDPGTGSEDPVLKTLEKETGLSRQTIAARAEQALSLLLHDY
                     SRFRVETIDSFFQSILRNLAKELGLGSSMNIELEEDEILEEAVDLMIEKAGENKELLK
                     WLQDYIEENLFEGRNRKIAPALKKFGETIFSESFQSREKELYRVLSDKSFLNDYREEL
                     FKLKAQANERLKGMGQRFFELIAQQGLEIDDLSNKNRGVAGYFLKLLNEEYKIDIFNT
                     TAQKALTDPEKWATAQHPRRAEIIEIAENTLIPFLQECEAERPKLYSIYHSCDLSLKH
                     IYKIGLLTDISLEVRAINREQNRFLLSDTAVLLKTLISDSDTSFVYEKAGTELQHIMI
                     DEFQDTSRVQWSNFKPLIFEGLSKNYDSLLVGDEKQAIYRWRNSDWRILGNLEKELAG
                     TELQFNTLNSNFRSEQRIVQFNNALFKQASLLSTASLENKLGIDCPDLALAYKEMEQQ
                     CSRKDEKGWVKAVFVRDDNNYYRHSLEQVVKQVEYLQEQGITPDQICILIRYKKHIPE
                     IAAYFAQYQSDHPDSPFCYTIVSDEAFRLDASPAVQILIAALKMLANPDDKLAREELE
                     QCLTRLLKTYGEEPDTFQEDLPADFESQLNELIHIPLFELADLLIRLFDLGKIPAQES
                     YLFSFM"
     gene            12376..14496
                     /locus_tag="PLI37_03905"
     CDS             12376..14496
                     /locus_tag="PLI37_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOQ58194.1"
                     /translation="MVLLFVNINGGLYAAGELSGQEERKIRSLISKMSLEEKAGLLHG
                     NSKFYVGAIPRLGIPELALSDGPHGVRAEINRDNWSYAGWTNDSSTYFPTGTALAATW
                     NPDLARLRGEVLGEEARFRGKDILLGPGVNIIRSPLCGRNFEYMSEDPYLTAQMTTAY
                     IRGLQSRDVAACVKHYLANNQETDRGSVDVYMSDRALHEIYLPAFKAAVQEAGTYSIM
                     TAYNKFRGLWCAEHPYLLDSLMRQELGFDGIFVSDWNGTHSTLASAKAGLDLEMGTDK
                     PYSEYYFAQPLIDAVRKGELPESLVDRKVAGILSLMMKLKIFDPAKRFEGSFNTPEHQ
                     QATYRSAAESIVLLKNDKGILPLDATKIKSLAVIGDNATRKQSFGGQSSEIKALYEVT
                     PLEGLKNRAKGRFEIRFAQGYEKSQRPSNRPDWALIDEAAALAAQCDAAVIMAGLNHD
                     YDTESRDKPDMALPYGQLELIRAVVKANPNTVVVIIAGSAVDMAGFNYRVPGLLWAWY
                     NGMEGGNALADVLLGEVNPSGKMPFSLPFCLDQSNAHALGEFPGRNRKVDYFDDIFVG
                     YRWFDTYGIAPLYPFGHGLSYTQFEYSESRADKEEWSMNEKLRLSLVLSNTGRFDGAE
                     VVQLYVSDPESELPRPQKELKAFQKVFLKAGESRELVFELPISDLAYYNDIKREWLLE
                     PGTIKCLLGSSSRDIRQEISIKVN"
     gene            14541..15563
                     /locus_tag="PLI37_03910"
     CDS             14541..15563
                     /locus_tag="PLI37_03910"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOQ58195.1"
                     /translation="MNKNINWSELSFGYLPTDYNVRCYFRRGKWGELEVSSSENITMH
                     MAATCLHYGQEAFEGLKAFRGKDNKIRIFRLDENAKRLQSSAETILMEKFPLEMFREA
                     VLKVVKLNEAYCPPYESGASLYIRPLLIGTGPRVGVKPADEYLFVVFVSPVGPYFKEG
                     FKPTNMVIMREFDRAAPQGTGHVKVGGNYAASLHSGEIAHAKGYSAVLYLDSKEKKYI
                     DECGPANFFLIRGNSYVTPASHSILPSITNKSLMQLAEDMGMEVIRRQIRVEELNDFE
                     EAGACGTAAVISPVARIDDADTGKSYVFSKDGKPGKVCEKLYHKLRAIQYGDEPDTYG
                     WTTFVE"
BASE COUNT         3586 a         3570 c         4005 g         4253 t
ORIGIN      
        1 acgaaattct gggtatgaac atctttaatt ccgtttttgt ggattgtacg gccagccccg
       61 aaatctcagc cctttacgcg gatttgttgt cacacaacgt gtctgtggta gcagccaaca
      121 aaatagcggc atcgtccgat tacgacaatt atgcacgact caaggaactg gcccgtcgca
      181 aaggggtgaa atatcttttc gaaaccaatg tgggagccgg tctgcccatt ataaatacga
      241 tgaatagctt gattcacagc ggagacagaa ttttaaaaat ggaagccgtt ttgtcgggca
      301 cgttgaattt tattttcaac accatcagtg ccgaaaatcc cctgagcaaa accataaaaa
      361 tggctcagga gaaacgtttt tcggagcccg atccccgcat cgacctgagc ggaaccgacg
      421 tcatccggaa attggtgatt ctggcacgcg aagccggtta cagagtagaa caggaagatg
      481 tggtgaaaaa tctctttatt cccgacaaat acttcaaagg cagtctggaa gatttttgga
      541 aaaacattcc cgagatggat gccgatttcg aagcaaaacg caagttgctc gagaaagatt
      601 ctctgcgctg gcgctttgtg gccaaaatgg aaaacggagc ttgttcggtt ggtcttcacg
      661 aagtcggacc atcgcatccc ttctacgatc tggaaggcag caacaacatt attctgatac
      721 agaccgagcg ctattttcag gatcccatgg ttatcaaagg ctacggagcc ggagccggtg
      781 taacggctgc cggagtgttt ggcgatataa tctccatcgc gaacattcgt taagatgaaa
      841 tacctgattg ttctgggaga cggcatggcc gacgaagcca tggagacctt gggcggaaag
      901 tcgcccatgc aggttgcctt tactccctgt atggaccggc tggccaggga gggtcgttgt
      961 ggccggctca ttacagtacc tgaaggtttt cctcccggca gtgaaattgc caatctgagt
     1021 ttactgggat acgatgtaag taagcaattc gaaggcagag gttcgctcga agccgccagt
     1081 atgggaatcg atgtccccga aggctatatg gcggcgcgct gcaatcttat ctgcatacag
     1141 gacggcaaga ttaaaaatca ttctgccggc catatttcta cttccgaagc cggggaattg
     1201 atagattttc tgaataagga gttaggcagt gaggatttgg tttttcatac gggtgtttcc
     1261 tatcgtcatc tcttgctgct caagggtggc aacaaagaat tggattgtac tccgcctcac
     1321 gatgtgtcgg gtaccgcctt tgccgaagtg ttgccacggg ccgtgcatcc ccgggcagaa
     1381 caaacagccc gtttacttcg cgaattgatt ttgcgatcgc aggagatttt gccggctcat
     1441 ccggtcaatt tggcccgcaa ggccagaggc ctggatacgg ctaattccat ttggccctgg
     1501 tcgccgggct accgtcccgc tatgctgccg ttgagggaaa agtatccgat acgaagcggt
     1561 tcggtgattt cggctgtgga tttgatcaag ggcacaggcg tttatgcagg cctggaaccc
     1621 atcgaagtgc ccggagccac cggtttgtac gacaccaatt acgaaggcaa ggctcaggcg
     1681 gccatcgacg aactcaagaa aaaagatttt gtgtttttgc atgtggaagc ttctgacgaa
     1741 gccagtcacg aggggaacgt cgatttaaag atccgcacca tagagtacct cgatcaacgc
     1801 ctgattcgcc ccatactgga ggagatttcc acttgggacg aggccgtcac catagctgtt
     1861 ttgcccgacc atcccaccta ctgccggacc aaaacccatg cggcagaggc ggttcccttt
     1921 ttgatttatc gcagcgatgc aggttctgcg caagctcggc ccaccaaagg cccgggcagc
     1981 ttttttagcc cggggaccgg tattttggag ccctccggca agctttttgt ccctgacgct
     2041 gtacaagtct tcgatgaatt ctcggtgcgg gagggctttt acggcacgct gcagggggag
     2101 gaatttatcc gtgccttttt cggcctgagt acgaaagcag acccttaatt acaaagaagg
     2161 aagggtttag atgttgaatt aacacagtca gacatttttt tcgatcaaca aataatgaac
     2221 tactacagca ccaacaaaca agctccgccg gtctctttac aagaagccgt ggtcaggggg
     2281 ctggctcccg accggggttt gtacatgccc gagcgcatca acactttgcc ggcttcgttt
     2341 tttgaagagc ttccgggcct gagctttcgg gaaatgtctt accgggtggc catggccttt
     2401 tttggagaag atcttcccga agatacgctc cgggagctgg tttacgacac gttgagtttt
     2461 gaaacgcctt tggttagggt tgacgagcat atctacagtt tggagttgtt tcacggccca
     2521 accttggcgt tcaaagacgt aggcgcccgt tttatggccc ggctgctggc ctattttatt
     2581 cgtcggcagg ggcaggagaa agtccatgtg ttggtagcca cctcgggcga taccgggagt
     2641 gccgtggcca atggcttttt gggagtggaa ggcattcagg tgcatgtttt gtatcccaaa
     2701 ggcctggtca gcgaaattca ggaaaagcag tttaccacct tggggcaaaa catcactgct
     2761 cttgagatag agggcaactt cgacgattgc cagcgcctgg tcaagcaggc ctttatggat
     2821 acggagctta accggcgctt tcatctgact tcggccaatt ccatcaatgt ggctcgtttt
     2881 ctgcctcagg ctttctatta tttcaatgcc tatgctcaat ggaagcgtta taacgatcct
     2941 tcgaagctgg ttttttgcgt ccccagcggg aatttcggta atatcacggc cggctttttt
     3001 gcctggcgta tgggattgcc cgtcgaacgt tttattgctt ccaacaaccg caacgatgtt
     3061 ttttatgaat acctgcaaag cgggcattac cggccacggg cctcggtagc cactttggcc
     3121 aacgcaatgg acgtgggcga ccccagcaat tttgcccgca tcctcgattt gtacgggcac
     3181 tcccatgagg ccattactgc cgaaatagcc ggagctcgct acgacgatca ggaaatccgg
     3241 gatacggttt accgtacttt taaagaaaag aactacctgc tcgatcccca cggtgcctgc
     3301 ggctacagag ccctggaaga gggtctgaaa ccgggcgaaa gcggcatctt tctcgaaacg
     3361 gctcatccgg ccaaattcaa agatacggtc gaggacatac tgggcgaggc tatagttgtg
     3421 ccaaaacgcc tgcaggcctt tatgcaggca aagaaacaga gtattcccct aagccctgcc
     3481 ttcgacgact ttaaagctta tttgatgcaa caaaaaggct ttaataaata aaaaatgcta
     3541 cattagcggc gctgaatttt aaatactatg gaacaatctt caatgtatcg ccctgagact
     3601 tcgagtcagg gactcagaga atttacgcta agagccttgc ttatagggct ggttctggcg
     3661 gtagtgctgg gagcagccaa cgcttactta gggctcaagg caggtatgac cattgctgcc
     3721 acttatccgg cagccgtaat cagtatggct gtattacgtt tttttaaagg cagcctgctg
     3781 gaagaaaatt ttgcccgtac ggtaggatcc atcggtgaat cggtggctgc cggggctatt
     3841 tttactctgc cggccttttt tattgccggt gtatggggcg gggaggaatt ttcttcctgg
     3901 agcagttata tgacggcttc gctcatcctg gttaccggcg gtgtgctggg agtgcttttt
     3961 gtggctcttt tgcgcagagt aatggtcgaa gacaaggaat taccttttcc agaaagtacc
     4021 gcagcagccg aaattcacaa aagcggacgc agcagtacgg gcgattccaa atacctggtc
     4081 tgggctatgg tgggcggagc tttggtaacg ctaggctctg ctttgaagct ttttgcttcg
     4141 agtgccaagg cttttttcaa ggtggccata gctaaattaa ccagcggaca caccatcgaa
     4201 ggaggttttt acgccgccgc tcccaatgtc agcccggcct ttttgggcgt gggttacatt
     4261 ataggaccaa gactggcctc gctgaatttc agcggctcgg taatggcctg gggtttgatg
     4321 gtcccactct tactggctgt attcggtcgc gattttgtga acggttttgc cgtggacggt
     4381 gttgccccca attctcagga ggcctggatg ctggccgcca atacggtatg gcagtcggtg
     4441 gtgagaatag tggccatagg cggaatgttg ctggcagcta tttttacgct ttatcgcatg
     4501 cgttccagcc tgactaccgg cttgaaacgt tcggtgaaag acttgagaaa agcggcttcc
     4561 gaaagcggag aggaagtggt aagaaccgaa aaagacctga aaacttcctg ggtattggca
     4621 ggcatagtgt tggccgccat tgctactttt tgcatcacct attttatttt caaaaccacc
     4681 gttctggtgg cggcggttgc tgctaccatt atgatagtgc tggccttttt cttttcggcc
     4741 gtttcgggct atctggtggg tttgatcggt tcgagcaata atcccataag cggcctgacc
     4801 atcactgccc tggtgattac cgccctgatt ttggtgcttt gcggggtaga cgaaaacacg
     4861 ggaggcgtag ctgccgtgct gggcgtggcc gccattgttt gtgtggctgc cgccgtggcc
     4921 ggcgaaatgt ttcaggacct caaggccggg catatcctgg gtggaacacc ctggaaaatg
     4981 cagctgggcg acatcatagg tgtggtgctc tccggttttg tgatgttcgg tgtgctgatc
     5041 attctcaata atgcagatat tgccatggga ggtatgcaag gttacgcggg aggttttggc
     5101 agcgaagccc tgccggctcc tcaggccagc ctgatggcca tactttccaa tgctgtggtg
     5161 gccggttcta tgacctggat tttgattatt gccggtatgc tgctttgtat agctttgatt
     5221 ttgatgggtg taaagagccc catgctggtc tttgtgggta tgtacctgcc gttccagacg
     5281 gtagctgcta tttttgtggg aggccttatc aagggcttgc tcgatatgcg tacagccaaa
     5341 atgaattatt caccggtaca actggccaaa gtcgaaaaca ccggcatttt gctcgcttcg
     5401 ggtatgatag ccggcgaagc cttgatggga ttgattattg ccattttcgc tctcttcaat
     5461 gttttcttct cggagatgtt gcccatagag caacccagtt attggatcgg cttggtgctg
     5521 atgttcgcga tggcctatct gatgatagta ttgcctctga aaaaagccgc caaaaccaat
     5581 ggccaaaaat aaaccccaaa atttattcga cctgataccc tctgtaaacg agaggatacg
     5641 catagaaaca ggggaagaca agcttttgca gctggtcttc ccccgtttca gatcagcctg
     5701 gttgaatcgt cgtctcactc ctaagggaag atcgccctat attcacatta agctcgaaga
     5761 gcacggatcg gccgttgtgc aattgataga cggccaacgt agcgtcagag agatcgccct
     5821 ggctctggca gagcatttcg aacaggaacc gggctacgag caccggatag ccgtttttat
     5881 tgagcattta cgtagagata actacatcag cctcaggcag ccggaggttc tttagttttg
     5941 cctgttgcca gggaaaaaga gtccttttat tgtccttttt tgaaccgata gtctgctttt
     6001 gcttaactta ggatattact tgcgtctgca aatagtattg aaatcacaat gctcgcatgt
     6061 ttcgagtacg gaggtttgat cgaagctttc tgcgggatcg aacagttcgc cgacgacgtt
     6121 ttgcagttcg gtttcgaaag tttccttgaa ttctccgaaa tcattcacca cattgctgcc
     6181 gttggtttta gccccggatt ttccggattt ttttacaact atatctgagc tgtaatcggg
     6241 cttgccggcc tgatgaatat atacgagttc gggtttaacc atcgaagcgg gatattgatc
     6301 cttaacgatg ccggcgtaaa gaaaggtctg aaagacgtgc ttgttgcgtt tttggtcgga
     6361 tttgaacaag cccttgtcga aggaagcgct ttcggctttg ccgccggttt tgtaatcgca
     6421 gatgcggata gtctcgtctt ttttatccat gcggtcgatg atgcctccca ctcgcagttt
     6481 tagtttcctg ccgggggtgg ggaggagttc catgtgtttt tctacgaaag tttccaggcc
     6541 caacaattcg aagggtgcgt agttttgatc cagtttgagt aagaatttca ggaactgaat
     6601 cagcaatcta cgttgaataa gttgttcgcc atttagtgcc ggcaggtttt gtccgtcttt
     6661 gagacggaaa aactctttgg ccataaaata atccactgca cgttccagac agtctttgtc
     6721 ggcgtataag gcttgcaggt cggcggcttc gattcttcca tttagctgtt caccggtgtc
     6781 gattctctcc tcgaactgtt tatctgtctc gattttctca ttgagccgct caccggtcgc
     6841 tgcattacgg tttaaaaacc cggccgggga atccggcgct ttcaaagcga gattgaaagc
     6901 ccggatatct tccatgcttg ccaaaatgtt ttcgtggctg tgcttacgtc cgtttttttg
     6961 tagaatcagt tttacataga ggtattgagc ggccgcatga aaaatagtac cgaaacacag
     7021 gggatccatt tgttcgctga tctcttcttc ttttttcagc ccccgcaggt attggaagta
     7081 gtattttaaa cggcaatcga ggtaggtatt caacatgctt ggggagaggt agcgcttttg
     7141 actgtttgtg tttgctgcgg cctggtcgta catatcgtaa agctgctgca taagggctgg
     7201 ggtcttgggg accagaatgg aagagcccgg gagggttttg atttccgaac tcaaatgcag
     7261 gatttcgatg ggcaacttgc tttctattgc caattgcagc ataaagcggc tcatttcgcc
     7321 ccggttcagg ccttcggtgg agctgttata caccagcgtt atgttttcgg ccctttgcag
     7381 cgctctgaaa aaataatagg cggaaatggc gtcctgatgc tccgggccgc tcagattgaa
     7441 cgccctgcgc agattgtagg gaataaacga attctcggct ctgtttttgg gcatttgccc
     7501 ttcgtttacc gagagcatca gtatgttttt gaaatcgaga caacgggttt cgagcattcc
     7561 cataatttgc aatccgttga gcggttcacc cgaaaaaggc acactcaggt caagtacctg
     7621 aagcaagagg tttttatagg tttgcaggtt gatttcgagc aggtcttttt tcaggagagc
     7681 gttcaggcgt tgcacttggg tatagctttt gaatagagct tcctggaaca aaggtaggta
     7741 gatttcgttg gttttgttgt ctgtttggcc ggtttccgta tttgtttcgt cgtcgttgtc
     7801 gatggtttga ctcaggaggc tgaacagctt gagcagcagt tctcccagct cgtaggccgt
     7861 ttttaccggg ctgaacagga ggctcaacaa atcatcttgt tgtaattcgt cgtatcccgg
     7921 atagaggcgg tttttaatgc gcagttcttt ttcgagagct gcagctttgg ggctgcattt
     7981 tcggatatag ggattttgca gcaaaggcaa tacatagcgg gagcgaaagg tttttttgtc
     8041 ccggttgtcg cagtgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcatg
     8161 ttcggtggaa gaatctatga accggatttt ttttgctgtt tgtttccagt tatcaaaaca
     8221 actttcgttc agctcattgg gaaaaagttt caggttttca cggataaaat gccccgcttc
     8281 gtgtccggga ttgtttagat aatagcgatc gtagtcccag tagaagcgtg ctttaccggc
     8341 tttctgcagc ttgccgaaca gaaacttttc gcagccgttc aaggcgttga aaccgacgaa
     8401 aagataggag ttggcttcga aagcttgctt atcgtcgaaa tgttcaacca cccaacgttg
     8461 caacatacct tcataggcta tattccgggc tgtcagctcc tctttgaact gagtatagat
     8521 gttgaataat tcattccaaa gccgaataaa gcgttttttc aattcgctgc gatctgtttc
     8581 gaaatgagca aagaaacgct gaatggccgc aatctgttcg ggcgataaat actcaaagtc
     8641 atcgctgtag gctgccagtt cgctgatgtt gcgaaacaat tgcccggcgt cggccaggtt
     8701 tttgtcgatg ttatcaaaat ccttgagcaa cagatttccc cagggataaa aatcgtcgaa
     8761 actttctttg tcgagtccct gtttttgata caccttaaac agaaggctgt tgagtaaaat
     8821 aggatcgcca agttccagcg ggcagcggag accgaaaagt tcgcttatac tgctgaattc
     8881 cggtgcccag atggctttcc cggcttgttt gtagagggcc tctacaaaaa acagcctggc
     8941 cctgttgttg gggaacacga tgcaaagttc attcaggctg tcggcatatt tgttgtagat
     9001 gtcctcggcg caatattgga gaaatgtttt catagttcta cctccccggc ttctacttgt
     9061 tccacctgtt gttcgtcgac ataccataga tagccttcca cctgtttatg ccccatccgg
     9121 cgaagcagtt ccatataccg ggccacttgc ctgcggtatt tcggcagggc tttgccgaac
     9181 ttgaaatcga tcaccaccat tttgtcttct tgtcgaataa ctctgtcggg acgcttttca
     9241 cgcaacttgt gctgagcgtc ttggtataaa ataacgcatt cattgtacag gcggtaattg
     9301 cccgaatacc attcttttgc ccggggatgt tccagggccg tttgagtata ttgcagcagc
     9361 ttgtcctgct cttcttcgtc aatcaaaccc tcgttcagca gcgaacgtac ggcggccgga
     9421 gcatccgact gctttttgat gcgtgaaaac aatttatgca gcagtttgcc acggtctatg
     9481 tagctgttgt gaaattcgcc tttgccgcct tccgacacaa aatcgcgcga aggattggat
     9541 tgccggaatc cggtttgacg gggaaaactt ctgtattcaa tggcaaggtc ctgttcagtc
     9601 ctcacttctt cgttcccgct tgaatcggta cagatcttgc cgtattcaaa accggcgaat
     9661 tgggtgtttt ccaccggatc gtctgttgta tctgcgattt cttcttcttt tcttcttcct
     9721 cctccttcca aaatcgattc atcctcctca tcgttttcga ctttaaagtg cggcgaaaac
     9781 aggggatgag ccgggtcgga cagtatttta taaattaatt cggccaccga ttttatctcc
     9841 tgattctttt ccgtgttttt tttgccttgc ccgggggctt tgctgcagat gattaaattt
     9901 ttttcggccc gcgtaaaagc tacgtacagc aagttgaggt tatcaacgaa ttgttgtacg
     9961 cattcttccc ggtaatcttc cttgaatatg gtcacattca taagttgttg gaaatttaca
    10021 ggaatcaaag gcaaagcggc gaaaggttgc aggnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnntccataa agctgaaaag ataactttcc tgagccggta ttttgccgag
    10201 atcaaaaagg cgtatcagca ggtcggccag ttcaaacaag ggaatgtgga tcaattcgtt
    10261 caactgtgac tcaaagtctg ccggtaagtc ttcctgaaag gtgtccggct cttcgccgta
    10321 ggtttttaaa agccttgtca ggcattgttc gagttcttcc ctggccagct tgtcgtcggg
    10381 attggccaac attttgagag cggcaatcag tatttgcaca gccggggagg cgtcgagacg
    10441 aaaagcctcg tccgatacta tggtatagca aaaggggctg tcgggatgat ctgactgata
    10501 ttgcgcaaag taggcggcaa tttcgggtat gtgtttttta taacgtatca atatgcaaat
    10561 ttgatccggt gtaattccct gttcctgaag atattctact tgcttgacca cttgctcgag
    10621 gctgtgccgg taataattgt tgtcgtctcg cacaaagacc gcttttaccc agcctttttc
    10681 gtctttccgg gagcattgtt gctccatttc tttgtaggcc agggcaagat ctgggcagtc
    10741 tattccgagt ttgttttcca acgaagctgt ggagagtagg ctggcctgtt taaaaagagc
    10801 attgttgaat tgaactatgc gttgttcgct gcgaaaattg ctgttaagtg tattgaattg
    10861 cagctccgtt ccggccagtt ccttttctaa attacccagg attcgccagt cgctgttgcg
    10921 ccagcgataa atggcttgtt tttcgtcccc caccagcagg ctgtcgtaat ttttggaaag
    10981 gccttcaaaa atcaggggtt tgaagttgga ccattggacg cgcgaagtgt cctgaaattc
    11041 gtctatcata atgtgttgca gctccgtgcc cgctttttcg taaacaaagg aagtgtcgct
    11101 gtcgcttatg agggttttca gcaagacggc cgtgtctgaa agcagaaaac ggttttgttc
    11161 ccggttgatg gccctgactt cgagggaaat gtcggtgagc aggccgattt tgtagatgtg
    11221 tttcaggctg aggtcgcagg aatgatagat ggaatagagt ttgggccgct cagcttcgca
    11281 ttcttgcaga aatggtatca gcgtattttc ggctatctct attatttcgg ctcgccgggg
    11341 gtgttgggct gtggcccatt tttcgggatc tgtaagggct ttttgagcgg tggtattaaa
    11401 aatatctatt ttgtattctt cgttgagcag ttttaaaaaa tagccggcta cccccctgtt
    11461 tttattggat aaatcatcta tttcaagccc ctgttgcgcg ataagctcaa agaaacgctg
    11521 ccccattcct ttcagccttt cgttggcctg ggctttgagt ttaaagagtt cctcgcgata
    11581 atcgtttaga aaggatttat cggagagcac ccgatagagt tctttttctc tggactgaaa
    11641 agattccgaa aatatagttt cgccgaattt tttgagggcg ggagctattt tgcgattgcg
    11701 tccttcgaac agattttctt cgatgtaatc ttgtaaccat ttgagtagtt ccttgttttc
    11761 ccccgctttt tctatcatca agtccacggc ctcttccagg atttcgtctt cttccaattc
    11821 aatgttcata gagctgccca agcccagttc cttggccagg ttgcgcagga tactctgaaa
    11881 aaaactgtcg atggtttcta ccctgaagcg gctgtaatcg tgtaacaaaa ggcttaaagc
    11941 ttgttctgcc cgggcggcga tggtttgccg cgacaggccg gtctcttttt cgagggtttt
    12001 cagcaccggg tcctcgcttc cggtgccggg gtctgaaagc agctgcaggt aatggagaat
    12061 acgctccttc atttcggtgg tggccttgtt ggtaaaagtc accgccaaga tatgccggta
    12121 ggattcgggg tttttaatta acagtttgat gtattccacc gacagacgcc aggtttttcc
    12181 cgagccggca gaggctttgt aaatattgat ccgggccatt tgctgttttc aatgctaatt
    12241 tcccctcaga gacagaaaat cggagaagaa ttcttatgtg ccaaagatag aaaattctta
    12301 cctttgcctc tcattctaca aggataaaaa ttgattcatt ctgcatccat gaaagccgta
    12361 ttttaccatt tatgtttggt tctgctgttc gtaaacatca acggcggact ttatgccgcc
    12421 ggcgagctgt cggggcagga agaaagaaag attcgctctc ttatctccaa aatgagcctc
    12481 gaagaaaaag ccggcttgtt gcacggtaat tccaagtttt acgttggggc cattccccgc
    12541 ttgggtattc ccgaattggc cctctcggac ggtcctcacg gagtaagagc ggaaatcaat
    12601 cgcgacaatt ggagttacgc aggctggacc aacgattcct ctacttattt ccccaccgga
    12661 accgcgcttg ctgccacctg gaatcccgat ctggcccgtt tgcgcggcga agttctgggc
    12721 gaagaggccc gtttcagggg caaagatatt ttgctgggtc ccggagtgaa cattattcgt
    12781 tctccgcttt gcggcagaaa cttcgaatac atgagcgaag atccttacct gacggcacaa
    12841 atgacaacgg cctacatcag gggtttgcag agccgcgatg tggcagcctg cgtcaagcat
    12901 tacctggcca acaaccagga gaccgacaga ggctcggtag atgtgtacat gagcgaccgg
    12961 gctttgcatg agatatattt accggcgttt aaagccgctg tgcaggaggc gggcacctat
    13021 tccatcatga cggcttacaa taagtttcgg ggactctggt gtgccgagca tccttatttg
    13081 ctcgacagtc ttatgcgaca ggagcttggt ttcgacggga tttttgtcag cgactggaac
    13141 ggcacccatt ctactttggc ttctgccaaa gcgggtttgg acctggagat gggcaccgat
    13201 aaaccgtata gcgaatacta ctttgcgcaa cctctgatcg atgccgttcg caaaggtgag
    13261 ctgcccgaat ccctggtcga ccggaaagtg gccgggattt tgagcctgat gatgaaactc
    13321 aaaattttcg atcctgccaa acgcttcgaa ggtagtttca atacccccga acaccagcag
    13381 gctacttatc gctcggcggc tgaatccatc gtgttgctta agaacgataa gggtatttta
    13441 cctctcgatg cgactaaaat aaaatccctg gccgtgattg gtgataacgc cacccgcaaa
    13501 caatctttcg gcgggcagag ttccgaaatc aaagccttgt acgaagtgac tcctttggaa
    13561 ggattgaaaa acagggccaa aggccggttt gagattcgtt ttgcccaagg ttacgaaaaa
    13621 agccagcgtc cttcgaacag acccgattgg gccttaattg acgaggcagc cgccctggcg
    13681 gcgcaatgcg atgcggctgt tataatggcc ggcctcaatc acgactacga taccgagtcg
    13741 cgcgacaagc ccgatatggc attgccatac ggtcaattgg agttaatcag ggcagtggtc
    13801 aaagccaatc ccaataccgt tgtggtgatt attgccggct cggcggtcga tatggccggt
    13861 ttcaattacc gggttcccgg tctgctgtgg gcctggtaca acggaatgga aggcggtaat
    13921 gccctggccg acgttctgct gggagaggtc aacccctcgg gcaagatgcc ttttagtctg
    13981 cccttttgcc tggatcagtc caacgcccat gctttaggtg aattccccgg ccgcaacagg
    14041 aaagtggatt atttcgacga tatttttgtg ggttaccgct ggttcgatac ttacggtatt
    14101 gctcctttgt atccttttgg tcacggcttg tcttacactc aatttgaata ctcggaatcc
    14161 cgtgcggata aagaggaatg gagtatgaat gaaaaactta ggctcagttt ggtcctcagc
    14221 aatacgggcc gcttcgacgg agccgaagtg gttcaattgt acgtttccga cccggaatcg
    14281 gaattgcccc gcccccaaaa ggagcttaag gcttttcaaa aagttttcct gaaagccggc
    14341 gaaagccgtg agttggtttt tgagttgcct ataagcgatt tggcctacta taacgacatt
    14401 aaacgggaat ggctgttgga accgggaacg attaagtgtt tgctgggctc ttcgtcgaga
    14461 gacatccgac aggaaataag tattaaggtc aactaagacc tgggatatca tattaataaa
    14521 gcgcaagata tataactgct atgaacaaaa acatcaattg gtctgaattg tcgtttggct
    14581 atctgcctac cgactacaat gttcgctgct attttcgcag gggcaagtgg ggagaactgg
    14641 aagtttcgtc ctccgaaaat attactatgc acatggctgc tacctgtttg cattacggtc
    14701 aggaagcttt cgaaggttta aaagccttta ggggtaaaga caacaaaata cggatttttc
    14761 gtctcgacga aaatgccaaa cgtctgcaat cttcggccga aaccatttta atggaaaagt
    14821 ttcccctcga aatgttcaga gaggccgtgc tgaaagtggt gaaattgaac gaagcctatt
    14881 gtccgcccta cgaatcggga gcttctctgt acatcaggcc tttgttgatc ggaaccggcc
    14941 cgagagtggg agttaaaccg gccgatgagt atttgtttgt ggtatttgta tcgcctgtgg
    15001 gtccctattt caaagagggc tttaaaccaa ccaacatggt aattatgcgg gaatttgacc
    15061 gtgctgctcc tcaaggtacc ggacatgtaa aggtcggcgg caactacgcc gccagtttgc
    15121 attccggcga aatagctcac gcaaaaggtt attcggctgt gttgtatctc gattcgaaag
    15181 aaaagaaata cattgacgaa tgcggcccgg ccaatttctt tttaatcagg ggcaacagct
    15241 atgtaacccc ggcttcgcat tccattctgc cctccattac caacaaaagc cttatgcaat
    15301 tggccgaaga tatgggcatg gaagtgatac gcaggcaaat ccgggtagaa gaactcaacg
    15361 atttcgaaga agcaggcgct tgcggcaccg cggctgtaat cagtccggta gcaaggatag
    15421 acgatgcgga taccggaaaa agctatgtgt tttccaaaga cggcaaaccg ggcaaagtct
    15481 gtgaaaaatt ataccataaa ctcagggcta ttcaatacgg ggatgagccc gatacctacg
    15541 gctggaccac ttttgtcgag tgaggctgta ccccttctat ggcaaaaaac aaactgaaaa
    15601 aatttgagga tctg
//
LOCUS       DAOSTD010000036        15474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_3921_length_15474_cov_16.978663, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000036 DAOSTD010000000
VERSION     DAOSTD010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15474
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3921_length_15474_cov_16.978663"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1325)
                     /locus_tag="PLI37_03915"
     CDS             complement(<1..>1325)
                     /locus_tag="PLI37_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOQ58196.1"
                     /translation="EGRRQIHKLRKDKINLIRSLDLLIIDEISMVRADLLDAVSDNLC
                     RYRRDSRPFGGLQLLMIGDVQQLAPVAKDDEWALLRTVYDSPYFFSSQALSKMPYTTI
                     ALTEVFRQSDAFFLNLLNKVRENKLDEAGLKALNSRHISGFNPDDNEGYITLTTHNYQ
                     AQSINEAKLAAIAQPEFCYKARLQGLFPESSFPTNQELLLKKGAQVMFVKNDSSPERL
                     YFNGKIGRVTDLCEDYICVQCEGDAREVVVQREQWFNTKYTLDPETKDIEETVEGVFE
                     QYPLKLAWAITIHKSQGLTFEKVIIDAASAFAHGQVYVALSRCKTLEGIVLNRPLNPW
                     ALKDDAVVSAFSREILNHPADPQHLLQAKRRYFVDRLLDLFDFQELLLALRLFNRQCR
                     DHLEALYPAYVADAGKACSACVNEITGVGLAFGNQISRISEACAEPEQEP"
     assembly_gap    1326..1425
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1426..1730)
                     /locus_tag="PLI37_03920"
     CDS             complement(<1426..1730)
                     /locus_tag="PLI37_03920"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOQ58197.1"
                     /translation="MQNNSLNTAAGVKATLLSYARSEKAAVLSRFFKTGPGQYGEGDL
                     FCGVTVPQVRSVARRALDIGRDEIEFLIQDDLHEVRQCGFFILVEQYRKQPPKRAEIV
                     "
     gene            complement(1712..2749)
                     /locus_tag="PLI37_03925"
     CDS             complement(1712..2749)
                     /locus_tag="PLI37_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOQ58198.1"
                     /translation="MNHIERFYATIRHQKVDYPASWLGIPDKAALPALYRHFGVDNMV
                     ALKAKINDDIFPVEMPYHSPTSNAIYAAFDFAKKKHGLPDERTLTAPGFFEDYSDPAD
                     VDKFDWPDPEKYIDPALCRKVVDEVPEGYAVMGVVWSAHFQDACAAFGMETALMNMML
                     APELFMAVTDRIVDFYLRANEIFYRATRGKLHAVLIGNDLGSQNGLMISPEAVREFVL
                     PGTRKLVQQAKRYGLEVMHHSCGSIHDVIPDLMEAGADIIHPIQALAAHMEPDRLKKD
                     FPSASFCGGVDAQYLLVHGNPDDVRAKVRELKALFPTGLIFSPSHEAILQDIPPANIE
                     ALFDEVCKTTP"
     gene            complement(2767..3465)
                     /locus_tag="PLI37_03930"
     CDS             complement(2767..3465)
                     /locus_tag="PLI37_03930"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOQ58199.1"
                     /translation="MPVFYAPDIADRTCLPPEESAHCVRVLRLKEGEEIRLADGKGHF
                     YRARISNADAKQCEVEILEVLPQASDSIGLHLAVAPTKNADRMEWLAEKLTELGIDSL
                     SFVCSFHSERKHLKTDRISKIAVAAMKQSERSLLPEIRDMVDFKQLLSRDFEGQKLIA
                     HCWPEEEKPFIKEVYLPGKPVLVLIGPEGDFSREEVEMAKSVGFQPISLGNIRLRTET
                     AAIFVATAIRLANM"
     gene            complement(3470..4075)
                     /locus_tag="PLI37_03935"
     CDS             complement(3470..4075)
                     /locus_tag="PLI37_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HOQ58200.1"
                     /translation="MSEKEISRILMTVWGLSYSRMKSGAYILILAEATGDRRLPIIIG
                     SSEAQAISFSLEKKTPPRPLTHDLMASMCHAFGLELKEVFISDCVEDVFYAELYFEHR
                     GEQITLGSRTSDAVALALRVGAPIYTTESMLRKRGMVFEETEQRGRHQMRIKKIRDEE
                     TWEDASDEELQAALQKAVEHEEYEQASLIRDELKKRKEAKS"
     gene            4382..4459
                     /locus_tag="PLI37_03940"
     tRNA            4382..4459
                     /locus_tag="PLI37_03940"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4417..4419,aa:Ile,seq:cat)
     gene            4650..6125
                     /locus_tag="PLI37_03945"
     CDS             4650..6125
                     /locus_tag="PLI37_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HOQ58201.1"
                     /translation="MNNNNVLILLDSLRDWAPYYETNSLLTVLDYLQYKNGEKSRKLV
                     INLSNDYSYNSEGYYCSLLAQTRGHQVIPGIDIINKLESGTGIRMDQNLQRICYQWIQ
                     AHEPAEPTWSIVIYFGTCKEKGLEKIARFIFEHYPCPILKVTFNTRSRNQIESVQAFP
                     LRELNDEQQTAFAEALDRFSQRVWQQPRSNKTSRFSLAILYDPEEKFPPSNKQALKKF
                     SEIAKKMRIHTEFITEEDANRLMEFDALFIRTTTSLNHYTFHLSQLAAQHGIAVIDDP
                     LSIIRCTNKVYLNELFEKEKIPVPPSMLILKSRRPSFEEITENIGEPFILKIPDGSFS
                     IGMVKIETEEALNEALDELFEKSAILLAQAFTPSEFDWRIGVLNGEPLYACKYYMAKG
                     HWQIYHHFQSGRSYSGTVETIPIYQVPRTVIETAVKAASLIGKGLYGVDLKMVKDKAM
                     VMEINDNPSIDHGCEDIVLGNELYYRILDHFERSLNNNERF"
     gene            6128..6568
                     /locus_tag="PLI37_03950"
     CDS             6128..6568
                     /locus_tag="PLI37_03950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOQ58202.1"
                     /translation="MLIRRASKSDLEAVLKIENSSFEAERFTRPQLLYLMTKARGLFL
                     VLELEAEIAAYLSILTHKKHHSLRIYSIAVDPAHRGMKLGQLLIEKAIAYALELQLPA
                     ISLEVNTRNSSAIALYTRNGFNTVEILKDYYGKGENALKMIKKL"
     gene            complement(6616..7227)
                     /locus_tag="PLI37_03955"
     CDS             complement(6616..7227)
                     /locus_tag="PLI37_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58203.1"
                     /translation="MSVNAQISRELVQNYIDLELANYPQATLIDLYKNYFQDAFGPGH
                     LMNDTNAAKDYLVKELAETDYCDTILFQPLGIHRDFYRINLSLVKNGTIALDTLLNGM
                     KESSVLARKPELSDWIIEWNQVLGIIQAYKPDIPNLQEDIQTIHRNLEKGIVVSHHSR
                     QFTEAYHPHYRIIHKSVVERWMTSERKGDGPGIQELIRLEPEK"
     gene            complement(7302..7799)
                     /gene="nrdG"
                     /locus_tag="PLI37_03960"
     CDS             complement(7302..7799)
                     /gene="nrdG"
                     /locus_tag="PLI37_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOQ58204.1"
                     /translation="MLRYLNYDIVFQEVPGEVSLAINITNCPNRCKGCHSPELMKDIG
                     EELNESVLLSLLEKYGKAISCICFMGGDASPKDVQHLATFLKKQTAFKIKVAWYSGRQ
                     ELPSNFEIVHFDFIKLGPYVQNLGGLNSLTTNQRFYRVENAKMIDDTKMFFKIGHSNF
                     LEKKY"
     gene            complement(7777..9867)
                     /gene="nrdD"
                     /locus_tag="PLI37_03965"
     CDS             complement(7777..9867)
                     /gene="nrdD"
                     /locus_tag="PLI37_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOQ58205.1"
                     /translation="MEVYIIKRDGKRELFSAEKIKDAIAKAFLSVGSFATQEVLGNIM
                     CRIHIYDGISVEEIQNQVEIALMAERYYAVAKSFMLYRQKHIEDREVRDKLKFLIDYC
                     DASNAASGSKYDANANVENKNMATLIGELPKSNFIRLNRRLLTDRLKEMYGKEVSDKY
                     IELLTNHFIYKNDETSLANYCASITMYPWLIGGTYSIGGNSKAPTNLKSFCGGFINMV
                     FMVSSMLSGACATPEFLLYMNYFIGLEYGRDYYKKVDAIVDLSRKQRSLDKVITDHFE
                     QIVYSINQPTGARNFQAVFWNISYYDRYYFESLFENFVFPDGSKPDWDSLNWLQKRFM
                     KWFNRERLRTVLTFPVETMALLTEDGEVRDKEYGDFTAEMYAEGHSFFTYMSDKADSL
                     SSCCRLRNEIQDNEFSYTLGAGGVSTGSKSVLTINLNRCIQYATKKGMFYMLFLEEVV
                     ELAHKAQLAYNENLRYMKEKGLLPLFDAGYINLGRQYLTIGVNGLVEAAEFLGIEIND
                     NPDYEYFVQQVLGLIEKINKKYRGKEVMFNCEMIPAENVGVKHAKWDKRDGYVSKRDC
                     YNSYFFVVEDSTLNVIDKFRLHGAKYIRHLTGGSALHLNLEEHLSKEQYRQLLRIAAQ
                     EGCNYFTFNIPNTICNSCGHIDKRKLNECPACTGRDLDYLTRIIGYMKRVSNFSQARQ
                     KEASNRYYAKVSQL"
     regulatory      complement(10017..10219)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10489..>11606
                     /locus_tag="PLI37_03970"
     CDS             10489..>11606
                     /locus_tag="PLI37_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOQ58206.1"
                     /translation="MMRVGDTYYMSSTTMHMAPGVPVMTSKDLINWKITSYCYDILDD
                     VDALNLVNGKSTYGRGTWASSIRYHKGLFYVSTFAQTTNKTYIYTTDDPEKGEWKKTS
                     FAPAYHDHSLFFDDDGRIYFIWGVGRLRIIELEADLSGVKEETERVLIENASAPAGSP
                     MGLQSEGSQLFKVNGRYYLFNICWPRGGMRTVVIHRAPSLEGPWEGRLALQDKGVAQG
                     GMVNTPDERWFAYLFRDYGSVGRIPYLVPIQWQDGWPVLGIEGKVPDELDLPAGKGLK
                     PGIVNDDEFNRKAGEPELPLVWQWNHNPDNSLWSLSERPGYLRLKTGRIDKSFLLARN
                     TLTQRSFGPQSSGSTLLDVSGMKDGDVAGLVALQRKFG"
     assembly_gap    11607..11706
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11707..12834
                     /locus_tag="PLI37_03975"
     CDS             <11707..12834
                     /locus_tag="PLI37_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOQ58207.1"
                     /translation="ENLIDWTDHGVILSQYDPPWVDSTSYSMWAPDCIERNGKYYFYF
                     PAHTKADENGRRSLGIGVAVADKPEGPYLPQTEPIKGARGIDPNVFIDKDGQAYLYWS
                     QQHIYVAKLKENMLELDSEPYIIPNLPEKGLKEGSWLFEREGIYYLTFPHVENKTERL
                     EYAIGKHPMGPFTMTGVIMDESPTACWTNHHSILEYRGRWYLFYHHNDYSPDFDKNRS
                     IRIDSLFFNEDGTIQKVQPSLRGVGLTPANTEIQLDRYSALSPDGAEIAYIDKNRPFE
                     GWKTILNKPGAWVQYNAVDFGNKKKKRVEIRALAAQGGILELRLNQPDGPLLAGLTLP
                     AGAEWENYDFKIKKTGGGIDNLIVLLRSESELEIDWLQFQP"
     gene            12889..14046
                     /locus_tag="PLI37_03980"
     CDS             12889..14046
                     /locus_tag="PLI37_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOQ58208.1"
                     /translation="MKKTSFTLVFLLISYFSFAQFAGMMRGQQAEALPEGFKPSSTNT
                     FLALYPAVNAQTRQALFKVVAPTANNVQVDLCNKKYDMTKDDRGVWTCMSDPQVVGFH
                     YYAILIDGVAVMDRNTKAFFGSNWMSSGIEIPEGPEGDYYRFDKNIPHGQVRSIYYWS
                     EINGMERHVNVYVPAEYEQKTDKSYPVLYLVHGWGEDENGWSNQGRMANIMDGLIASG
                     KAVPMIVVMPSGDIQTNPDVRRASGDISQIFVQDLLPFIDRNFRTKTDKQNRAMAGLS
                     RGGFQTCQTVSTNLDKFGWMGTFSGFFIRGDNSLETAFNGLFKDPSEFKRQIKLLYIS
                     TGTEENTPKAQVEALKAKGIDNIVYSESQGTAHEWLSWRRALNEFAQLIFK"
     gene            14099..>15474
                     /locus_tag="PLI37_03985"
     CDS             14099..>15474
                     /locus_tag="PLI37_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOQ58209.1"
                     /translation="MKPTPFFSGVFKLPALLLLFAVITPPLYAQKAVKASGTPLFTDF
                     EYQGNDKIYQNHPLKPDEFYNPILQGCYPDPSITRKGDDYYLVCSSFAMFPGVPIFHS
                     LDLVNWTQIGHVLDRKSQLIIHNCGISAGIYAPQIIYNPYNDYFYMITTQISGGIGNM
                     VVKTKDPHQGWSEVIKLDFNGIDPSLFFDDDGKAYVVHNDGPDRGKELYNGHRVIKIW
                     EYDTEQDKIIPGSDKIIVDGGVDISKKPIWIEAPHIYKKNNKYYLMCAEGGTGGWHSE
                     VIFVSDSPKGPYIPAPGNPILTQRHFAGDRANKVDWAGHADLVLGPDNKTYYGVFLAV
                     RPNEKNRVNTGRETFILPVDWSGEFPVFVNGLLPMEPKLKMPQGVTENNTGKENFLPN
                     GNFSFSDNFSADKPDYRWISLRGPREEFIETSKQGLKIKPYPVNIKEVKPTSTLFLRQ
                     MHNSFSFTV"
BASE COUNT         4133 a         3813 c         3383 g         3945 t
ORIGIN      
        1 ggctcttgtt ccggctcggc acatgcttcg gatattcgac tgatttggtt tccaaaagca
       61 aggccaacgc cggttatttc gttgacacag gcagaacagg ccttgcctgc atctgccacg
      121 taggccggat ataaagcttc caggtgatcg cgacattgcc ggttaaacaa acgcaaggcg
      181 agcaaaagct cctgaaaatc gaataaatcg agcaaacgat caacgaaata cctccgcttg
      241 gcttggagca agtgttgcgg gtcggccgga tggttcagaa tctctctgct gaaagcgctc
      301 accacggcat cgtccttgag cgcccaaggg ttcagggggc gattgagcac tattccctcg
      361 agggttttgc atcgactcaa ggccacatag acctggccat gggcaaatgc agaagcggca
      421 tctataatca ctttttcaaa ggtcaggccc tggcttttgt gtatggtgat ggcccaggcc
      481 agtttgagcg ggtattgttc aaaaacgcct tcgactgttt cttcgatatc tttagtttcg
      541 ggatccaggg tgtatttggt gttgaaccat tgctcacgct gaaccacgac ttcccgggca
      601 tcgccttcgc attgcacaca tatataatcc tcgcacagat cggtaacccg gcctattttc
      661 ccgttgaaat acaacctttc gggagaggaa tcgtttttga caaacatcac ctgagctccc
      721 tttttgagca gcaattcctg attggtggga aaagatgatt cggggaacaa accctgcaac
      781 ctggccttgt agcaaaattc gggctgggca atggctgcca gtttggcttc gttgatcgat
      841 tgggcctgat aattgtgcgt ggtcagggtg atgtaaccct cgttatcgtc gggattgaaa
      901 ccacttatgt gtctgctgtt gagagctttc aaaccggctt cgtcgagctt gttttcgcgc
      961 actttattga gcagattcag aaaaaaggca tccgattgcc tgaatacttc ggtaagggct
     1021 atggtggtgt aaggcatttt cgacaaagcc tgactactga aaaaataggg agaatcgtac
     1081 accgtacgta agagagccca ttcgtcgtct ttagccaccg gggccaactg ctgcacatcg
     1141 cctatcatca gcagttgcaa accgccaaaa ggccggctgt ctctgcggta acggcaaagg
     1201 ttgtcgctga cggcatccag caggtcggca cgtaccatac ttatttcgtc gataatcaag
     1261 aggtcgaggc tgcggataag attgatttta tccttgcgca acttatgaat ttgtcgtctg
     1321 ccctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnactat ttcagctcgc
     1441 tttgggggct gtttgcgata ttgttccacc agaataaaga atccgcactg acgtacttcg
     1501 tgcaggtcgt cctgaatcag gaactcaatt tcgtctctgc ctatatccaa agcccgccgg
     1561 gccacagaac gtacctgagg caccgtcacc ccgcaaaaaa gatcgccctc gccgtattga
     1621 ccgggaccgg ttttgaaaaa gcggctcaac acggcggctt tttcgcttcg ggcataactc
     1681 aaaagggttg cttttacccc ggcagcagta ttcagggagt tgttttgcat acttcgtcga
     1741 atagagcttc aatgttggcc gggggaatat cttgtagtat ggcttcgtga ctgggagaga
     1801 aaatcagacc ggtcgggaaa agagctttga gttctctcac cttggcccgc acatcgtcgg
     1861 gattgccgtg caccaacaag tactgagcat caacccctcc gcaaaagctg gcagagggaa
     1921 aatccttttt gagcctgtcc ggttccatat gagcagccag agcctgtatg ggatgaatga
     1981 tatcggcccc tgcttccatc aggtcgggaa tcacatcgtg gatggaaccg caggaatgat
     2041 gcatcacttc caacccgtaa cgtttggctt gctgtaccag ctttcgggta ccgggcaaaa
     2101 caaactccct gacggcttcg ggcgaaatca tcaggccgtt ctgactgccc aggtcgttgc
     2161 caatcaaaac ggcatgcagt ttgccccggg tggcacggta aaaaatctcg ttggccctaa
     2221 ggtaaaaatc cactatcctg tccgtaacgg ccataaacaa ttccggggcc aacatcatat
     2281 tcatcagggc cgtttccatg ccaaaagcgg cacaggcatc ctgaaaatga gccgaccaaa
     2341 ccacgcccat aacggcataa ccctcgggca cttcgtccac tactttgcgg caaagcgccg
     2401 gatcgatgta tttttcgggg tcgggccaat cgaatttatc cacatcggcc ggatcgctgt
     2461 agtcttcgaa aaagccggga gccgtcaggg tgcgttcgtc gggcaagccg tgctttttct
     2521 tggcaaaatc gaaagcagcg tagatggcgt tactggtggg cgaatggtaa ggcatctcca
     2581 caggaaaaat atcgtcgttt attttagcct tgagagccac catattgtcc acaccaaaat
     2641 ggcggtacag ggcaggtaat gccgctttgt cgggtatgcc cagccaggaa gcgggataat
     2701 ctaccttttg atgtcttata gttgcataaa agcgttcaat gtgattcata gtatgttaga
     2761 aaaaagttac atattggcca agcggatggc ggtagccaca aatatggcgg ccgtttcggt
     2821 gcgcaaacgg atgttgccca gggaaatggg ttggaagccc accgatttgg ccatctctac
     2881 ctcctcacgg ctaaagtcgc cttcgggccc tatcaatacc aaaacaggct tgccggggag
     2941 gtaaacttct ttgataaagg gtttttcttc ttcgggccaa caatgggcta tcaatttctg
     3001 tccttcaaaa tcgcggctca gcaattgttt aaaatctacc atgtcgcgga tttcaggcag
     3061 gagagatcgt tcggactgct tcatggcagc cacggcaatt ttagagatgc ggtcggtctt
     3121 gagatgtttg cgctcggaat ggaaactgca tacaaagctc aggctgtcga tgcccagttc
     3181 ggtcagcttc tcggccaacc attccatacg atcggcgttc ttggtgggag ctacggccag
     3241 atgcaagcct atggaatcgg aggcctgggg taacacttcc aatatttcca cttcgcattg
     3301 cttcgcatct gcgttactga tgcgggcgcg ataaaaatgc cccttaccgt cggccaaacg
     3361 aatttcttcc ccttccttca ggcgtaaaac cctgacacaa tgggccgact cctcgggagg
     3421 aagacaagtc cggtctgcaa tatcgggagc ataaaatacc ggcataaggt caggatttgg
     3481 cttctttgcg tttttttaat tcatccctta tcagggaagc ctgctcgtat tcttcatgct
     3541 caacagcctt ttggagcgct gcctgcaatt cttcgtcgct ggcgtcttcc caggtttcct
     3601 catcgcgtat ctttttgata cgcatttgat gccggcccct ctgttctgtt tcttcgaaaa
     3661 ccatgccccg cttgcgcaac atactttcgg tagtataaat aggagcgccc acccgcaaag
     3721 ccagggccac agcatcggaa gtcctggaac ccaaggtgat ttgctctccc ctgtgctcaa
     3781 aatagagctc cgcgtaaaac acatcttcca cgcaatccga aataaacact tctttgagtt
     3841 ccagcccgaa ggcatgacac atagaagcca tcaaatcgtg ggtaagcgga cgcggaggcg
     3901 tttttttctc caacgaaaac gaaatggcct gagcctcgct ggaacctata atgatgggta
     3961 aacgccggtc tcccgtagcc tcggccaaaa tcaggatata agcacccgat ttcatacggc
     4021 tgtaggatag gccccagacg gtcatcaaaa tacgcgaaat ctctttttcc gacataatac
     4081 tattcttttt attttgaggg ttcagtcctg tcagacaaca agctctttga gttaccatct
     4141 gcttttttat cctgctttag cgtcagtcgt ttacacctaa aaatcgggat tcgagcgctc
     4201 tgaagctttc cggatattcc tgtacactaa gcgacaacgc cgatttgaca gagtttaccg
     4261 aaccgtgtac aaagataaga gatgactgag caaaatccaa aagaaaaatt gtgttttgcc
     4321 cttggctatt tcactaaaaa gcagtaatat tgcagccgct ttccggagac agttccaaag
     4381 cgggcccata gctcagtcgg ttagtagcac ctgactcata atcagggggt cactggttca
     4441 agtccagttg ggcccaccaa tcaaagccaa gcagttacat gaatttgtga ctgctttttt
     4501 gttataatta ccctcctatc ctcctgaaac tgtatttttt tactccccgg aacaattatt
     4561 caggaatttc tctacctttg gggcgctgaa aaaaatgaaa aggcagccct ttttcggtgt
     4621 ttataatgtg ctgcttatca gatgtttgta tgaacaacaa caatgtgttg attctgctcg
     4681 attccctccg cgactgggcc ccttattacg agaccaacag cctgcttact gttctggatt
     4741 acctgcaata caaaaacggc gaaaagagcc gcaagctggt tattaatctg agcaacgatt
     4801 acagttacaa ttccgaaggc tattactgct cgctgctggc ccaaaccaga ggacatcagg
     4861 tgattcccgg catagatatc atcaataaac tcgaaagcgg caccggtatc cggatggatc
     4921 aaaacctgca gcgaatttgc tatcaatgga tacaggcaca cgaaccggcc gagcccacct
     4981 ggagcatagt tatctacttt ggcacctgca aagaaaaagg cctggaaaaa atagcccgtt
     5041 ttatttttga gcattatccc tgccccattc tgaaagtcac tttcaataca cgctccagaa
     5101 atcaaattga aagcgtgcag gctttcccgc tgagggaact caacgacgag cagcaaaccg
     5161 cctttgccga ggctctcgat cgtttcagtc aacgggtctg gcaacaaccc cgttccaata
     5221 aaaccagccg ctttagcctg gctatacttt acgatcccga agaaaagttt ccacccagca
     5281 acaagcaggc gctgaaaaaa ttcagcgaga tcgccaaaaa aatgcgtatt cataccgaat
     5341 ttattaccga agaagacgcc aatcgcctga tggagtttga tgcgctgttt atccgcacca
     5401 ccacctcgct caaccactat acgtttcatt tatcgcaatt ggctgctcaa cacggaatag
     5461 cggtaatcga cgatcccttg tccattattc gttgcaccaa caaggtctat ctgaacgaat
     5521 tgttcgaaaa agagaagatt ccggttcctc cctccatgct tattcttaag tcgaggcgtc
     5581 cctcgtttga agaaattaca gaaaacatcg gtgagccttt tatcctgaaa atacccgacg
     5641 gctccttctc gataggcatg gttaaaatcg aaacagagga ggctctgaac gaagctttgg
     5701 acgaactctt tgaaaaatcg gccattctgc tggcccaggc ttttactccc tccgaattcg
     5761 actggcgtat aggcgtgctc aacggagaac ccctctatgc ctgcaaatat tacatggcca
     5821 aagggcattg gcagatttat caccacttcc agtcgggacg cagttacagc ggcacggtcg
     5881 aaaccatacc tatttaccag gtgccgcgca ccgtgatcga aaccgccgta aaagcggcct
     5941 cattgattgg taaaggcctg tacggagttg atcttaagat ggtcaaagac aaggccatgg
     6001 tgatggaaat caacgacaat cccagtattg atcacggctg tgaagatatc gtgctgggca
     6061 acgagcttta ttaccgcatt ttggatcact ttgaacgatc actgaacaac aacgaacgat
     6121 tctgactatg ctgattcgcc gggcaagcaa aagcgacctg gaagctgtgc tcaaaataga
     6181 aaacagttcc ttcgaagcgg agcgctttac gcgccctcag ttgctttatc ttatgaccaa
     6241 agcccggggc ttgtttctgg tgctggaact tgaagcggaa atcgctgctt atctctctat
     6301 tttaacacat aaaaaacatc acagcctgcg catatattcc atcgccgttg accccgccca
     6361 ccggggaatg aaactgggtc agttgttgat cgaaaaagcg atagcttacg ccctggagct
     6421 ccaattaccg gccatcagcc tcgaagtgaa tacccgaaac agctcagcca tagccctgta
     6481 cacccgcaac ggttttaata ccgtggaaat tctgaaagat tattacggca agggagaaaa
     6541 tgcgctgaaa atgatcaaaa agctctgacc gttcaacact tcgctgcaat ttgttccgaa
     6601 gcgatgagct ttaatttatt tttcaggttc taagcggatt aattcttgta ttcccggtcc
     6661 atctcccttt cgttcagatg tcatccatct ctctaccacc gatttgtgaa taatgcgata
     6721 atgcgggtga taggcttcgg tgaattgccg gctgtggtga gaaacaacga ttcccttctc
     6781 taaatttcga tggattgttt ggatgtcttc ctgcaaattc ggaatatcag gtttgtacgc
     6841 ttgaatgatg ccgagtacct gattccattc gatgatccaa tcggacaatt cgggttttcg
     6901 tgccaatacc gaactttctt tcatgccatt cagcaatgtg tcgagagcta tggtgccgtt
     6961 tttgaccaga cttaggttta ttctgtaaaa atcacgatga attcccagag gttggaaaag
     7021 aatcgtatcg caataatccg tttctgccag ttctttaacc agataatctt ttgccgcgtt
     7081 cgtatcgttc attaaatgcc ccgggccaaa ggcatcctga aaataatttt tatacaaatc
     7141 aatcaaggta gcctgcgggt aattggcaag ttccaaatca atataattct gaacaagctc
     7201 ccggctgatt tgtgcattta cgctcatcgt gcaaaataag agggctgttg tgaaaaatat
     7261 ttttatgcgc attgcaaaaa acaaagaatt acaaatatcg cttaatattt tttctcaaga
     7321 aagtttgaat gaccaatttt gaaaaacatc ttggtatcgt caatcatctt tgcgttttca
     7381 accctataaa agcgttgatt ggttgtaaga gaattcaggc ctcccaagtt ctgcacataa
     7441 ggtcccaact tgataaaatc aaaatgaaca atctcaaaat ttgaaggcaa ttcttgtctt
     7501 cccgagtacc aggcaacttt tattttgaat gccgtttgtt ttttcaagaa tgtcgccaaa
     7561 tgctgcacat ctttagggga agcatctcca cccataaaac aaatacaact aatcgctttt
     7621 ccgtattttt cgagcaaact caaaagtacc gactcattca gttcttctcc gatgtctttc
     7681 atcagttccg ggctgtggca gcctttacat ctgttgggac aatttgtgat atttatagcc
     7741 aaactcactt cacctggaac ttcttgaaaa actatatcat aattgagata ccttagcata
     7801 atacctattc gaagcttcct tctgtctggc ttgagaaaaa ttactgactc gtttcatgta
     7861 cccgataata cgggttaaat aatccaaatc tcggccggtg catgccggac actcgttcaa
     7921 tttgcgttta tctatatgcc cacaactatt gcaaatagta ttgggtatgt tgaatgtgaa
     7981 ataattacat ccctcctgcg cagctattcg caaaagctgg cggtattgtt ctttactcag
     8041 atgctcttca agattgaggt gcaaggcaga tcctccggtg aggtgtcgta tgtattttgc
     8101 accgtgcaaa cgaaatttat ctatcacatt taatgtgctg tcttctacaa caaagaaata
     8161 gctgttataa caatctcttt tggaaacata gccatcgcgc ttatcccatt tagcatgctt
     8221 aacccctaca ttctcggcag gaatcatctc gcaattaaac atcacctcct tgccccggta
     8281 ttttttgtta atcttctcta tcaatccaag cacttgctga acaaaatatt cgtaatcggg
     8341 attatcattg atttcgatcc ctaaaaactc ggcagcttca actaaaccgt tcacgccaat
     8401 agtaaggtat tgacgtccaa gattaatata acctgcgtca aaaagaggca gcaatccttt
     8461 ttctttcata taccgcaggt tttcgttata tgccaactgt gctttatggg caagttccac
     8521 aacctcctcc aaaaacaaca tataaaacat cccttttttg gtggcatatt gaatacaacg
     8581 attcaaattg atcgtcagca cactcttgga tccggtcgaa acacctccgg ctccgagcgt
     8641 ataactgaac tcgttgtctt gaatttcatt gcgcagcctg cagcaactac tcagcgaatc
     8701 ggccttatcg ctcatataag tgaaaaagga atgaccttcg gcatacattt cagcggtaaa
     8761 atcaccatac tccttgtccc ttacctctcc gtcttctgtc aacaaagcca tagtctcaac
     8821 aggaaaagtc aagaccgttc gtaagcgttc gcggttgaac catttcataa aacgcttttg
     8881 taaccaattg agcgaatccc aatcgggctt gctgccatcg gggaacacaa aattttcaaa
     8941 taaactttca aaatagtacc tgtcgtagta cgaaatattc caaaacacgg cttgaaagtt
     9001 acgtgcgccg gtaggttggt tgatagaata tacaatctgc tcaaaatgat ctgtaattac
     9061 tttgtccaaa gagcgttgtt ttctggacaa atctactatt gcatctactt ttttataata
     9121 atctctaccg tattccaagc cgataaaata attcatatac aacaaaaatt ccggtgtggc
     9181 acaagcaccg ctcaacatgc tggaaaccat aaagaccata ttaataaagc ctccgcagaa
     9241 agatttgaga ttggttggag cttttgaatt tcccccaata gaataagttc ctccaatcaa
     9301 ccatggatac atggtaatac tggcacaata attggccagg ctggtttcat cgtttttata
     9361 aataaaatga ttcgtcagca attcaatgta tttgtcggac acttctttac cgtacatttc
     9421 tttcagcctg tcggtcaaca atcttcgatt taatcttata aagtttgatt taggtaattc
     9481 gccaatgagt gtcgccatat ttttattttc cacattggcg tttgcatcat acttactgcc
     9541 ggaggcggca ttggatgcat cgcagtaatc gatcaggaac ttgagtttat cgcgcacttc
     9601 acggtcttcg atgtgttttt gacggtaaag cataaaagat tttgctaccg cataataccg
     9661 ttcagccatt aacgctattt ctacctgatt ctgaatttct tccacagaaa ttccatcgta
     9721 aatatgtatc cggcacataa tattgcccaa cacttcctga gttgcaaaac tccccaccga
     9781 aagaaatgct tttgcaatgg catctttaat cttctcagcc gaaaaaagct cccttttccc
     9841 gtctctttta ataatgtaca cttccatgac gtatctgttg aattaatatc atcgttcaac
     9901 gagagatcgg ggaaaagaaa acggcctata ccaaagacaa atcagcaact ttgatcaggt
     9961 gtttattaaa aaacaaatga atcgtaagtc cgccaatcgc tcttcacccg aaagctctcg
    10021 gtaaaaaaac atcaggcagg tcttctgact tgtttcgggc agtgcgcctt cccatccaaa
    10081 acgacagtgg ccgagaatac actccttact ttagaaactc acagctacgg ggatagttcc
    10141 tgactttcac aggattccct tttaatcccg gattgcgaat aaaaagcaat ctctgagaac
    10201 caaatgctgg gaacaaagat atacaaattt accttagtgg cataaatata attctgaatt
    10261 tacattcaga tatgtgcaac aaactctttt ggcaaaggca gaaaaaatgt atattcgttg
    10321 cgaattttaa agttttttgt ttcaaccaac catataaaat ttaaccttat gatctgccaa
    10381 cgccttttgc ttttttcttt ctgcgcttta tgtttgttaa gcgccaatga aagccccgct
    10441 cagaaagccc aaaatcccgt catttttgcc gacgtgcccg atatgtctat gatgcgtgtg
    10501 ggcgatactt actacatgag cagcaccact atgcatatgg ctcccggggt gcctgttatg
    10561 acatcaaaag acttgattaa ctggaaaata accagttact gctacgatat actcgacgat
    10621 gtggacgcac tgaacctggt caacggcaag agcacctacg gcagaggcac ctgggccagc
    10681 agcatacgct accacaaggg gctcttttac gtttccactt tcgctcaaac caccaacaaa
    10741 acctatatat acaccaccga cgatcctgaa aagggcgaat ggaaaaagac ttcctttgct
    10801 ccggcttacc acgatcacag cctgtttttt gacgatgacg gccgcattta ttttatctgg
    10861 ggggtgggcc gtttacgcat catcgagctt gaggccgatc tgagcggcgt gaaggaagaa
    10921 acggaacggg ttttaataga aaacgccagc gcgccggcag gcagcccaat gggcctgcag
    10981 tccgaaggtt ctcaactgtt caaagtaaac ggcaggtact atttgttcaa tatttgctgg
    11041 cccaggggtg gtatgcgcac ggtggtgata catcgtgccc ccagtctcga gggcccctgg
    11101 gaaggacgtt tggctttgca ggacaagggt gtggctcagg gcggtatggt caacacaccc
    11161 gacgagcgct ggtttgccta tcttttccgc gactacggtt cggtaggccg gatcccttat
    11221 ctggttccga tacaatggca agacggctgg ccggtgcttg gaatagaggg caaagtgccc
    11281 gacgaactgg atctgccggc cggtaaaggt ctgaaaccgg gcattgtcaa cgacgatgaa
    11341 tttaatcgta aagccggtga acctgaactg cctctggtct ggcaatggaa ccacaatccc
    11401 gacaacagcc tctggtcgct gagtgaacgc cccggctatt tacgcttgaa aacgggacgc
    11461 atagacaaga gttttttgct ggcccgaaac acgctgactc aacgcagttt tggtccccaa
    11521 agttcgggca gcaccctatt ggatgtttca ggaatgaaag acggcgatgt agccggattg
    11581 gttgccctgc agcgtaaatt cggacannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnngaaa atctcatcga ttggaccgac catggcgtga tcctcagcca gtacgatccg
    11761 ccctgggtcg attctacttc gtacagcatg tgggcccccg attgcataga gcgaaacggg
    11821 aaatactact tttattttcc ggcccacacc aaagcagatg aaaacggccg cagaagtctt
    11881 ggcatcggag tggctgtagc cgacaagccc gaaggccctt acctgcccca gaccgaaccc
    11941 atcaaaggag cacgcggaat agatccgaac gttttcatcg acaaagacgg ccaagcctac
    12001 ctatattggt cacaacaaca tatttatgtg gccaaattga aggagaatat gcttgaactg
    12061 gattccgaac cttatattat ccccaattta ccggaaaaag gacttaaaga agggtcctgg
    12121 ttatttgaaa gagagggaat ttactacctg actttccccc atgttgaaaa caagacggaa
    12181 cgcctcgaat acgctattgg taagcatcct atgggtccct ttaccatgac gggggttatt
    12241 atggacgaat cgcccacggc ttgctggacc aaccatcatt ccattttgga ataccggggc
    12301 cggtggtatt tgttttatca ccacaacgat tattcgccgg attttgataa aaaccgctcc
    12361 atccgtatcg acagtctttt tttcaatgag gacgggacta ttcaaaaagt acagcccagt
    12421 ctgcgtggag taggattaac cccggccaat accgaaatac aactcgaccg gtattctgcc
    12481 ctcagtcccg atggtgccga aatcgcttat atcgacaaaa acaggccttt cgaaggctgg
    12541 aaaacgatat taaacaaacc gggagcctgg gtgcagtaca acgccgtcga ttttggcaat
    12601 aaaaagaaga aaagggtaga aatcagggct ctggcagcac aaggcggcat cctcgagcta
    12661 aggctcaacc agcccgacgg gcctcttttg gccggcctta cactcccggc cggagctgaa
    12721 tgggaaaact acgacttcaa aattaaaaag accgggggcg gtatagacaa cctgatcgtt
    12781 ctgctccgtt cggaatctga gctggaaatt gactggctcc aatttcaacc ctaaacccta
    12841 aatttgacta tctaactaat tgaaccacca actttaatag tttaaatcat gaaaaaaact
    12901 tcttttactt tagtctttct gctgataagc tatttcagct ttgcccaatt tgccggcatg
    12961 atgcgcgggc aacaggcaga agccctgccc gaaggcttca aaccttcttc gaccaatacc
    13021 ttcctggctt tgtacccggc cgtcaatgct caaacccgcc aggcgctctt taaagtggtg
    13081 gctcccaccg ccaacaatgt tcaggtggat ttgtgcaata aaaaatacga catgaccaaa
    13141 gacgacagag gggtatggac ttgtatgtcc gatcctcagg tggtcggttt tcactattac
    13201 gctattctca tcgacggcgt agcggtgatg gatcgcaaca ccaaggcctt tttcggtagc
    13261 aactggatgt cgtcgggcat cgaaattccc gaagggcccg agggcgacta ctaccgtttc
    13321 gacaaaaaca ttccccacgg tcaggttcgt tccatctatt actggtccga gatcaacgga
    13381 atggaacgcc acgttaacgt ttatgttccg gccgaatacg aacaaaaaac cgacaagtcg
    13441 tatcccgtac tctatctggt acatggttgg ggcgaagacg aaaacggctg gtccaaccaa
    13501 ggccgcatgg ccaacattat ggacggactt attgcctcgg ggaaggcggt acccatgatc
    13561 gtggttatgc ccagcggtga cattcaaacc aatcccgacg ttcgccgggc cagtggagac
    13621 atcagtcaga tatttgttca ggatttactg ccttttatcg accggaactt cagaaccaaa
    13681 acagacaaac aaaaccgagc catggccggc ctttcgagag gtggctttca aacctgccag
    13741 acggtctcca ccaacctcga taaatttggc tggatgggaa ccttcagcgg attttttata
    13801 aggggcgaca acagcctcga gacggctttc aacggtttgt ttaaagatcc ttcggaattc
    13861 aaacggcaaa tcaagctgct ctacatcagc acaggcaccg aagaaaacac acccaaagct
    13921 caggttgaag ccctcaaagc caagggcatc gataatattg tttattcgga atctcaggga
    13981 acggctcacg aatggctcag ttggcgcagg gcattgaatg aatttgccca attgatcttt
    14041 aagtaatccg acccggttca aatcccatta ataagaaaaa caaccattat ttcaacgtat
    14101 gaaacctact ccattcttct ccggtgtttt taaactaccg gcactgctcc tgttatttgc
    14161 tgtaatcact cccccgctct atgctcaaaa agcagtcaaa gcttccggga ctcccctgtt
    14221 taccgatttt gaatatcagg gcaacgacaa gatttaccag aaccatcctc tgaaaccgga
    14281 cgagttttac aatcccattc ttcagggttg ttaccccgac ccgagcatca cccgcaaagg
    14341 agacgattat tatttggttt gctcctcttt tgctatgttt cccggagtgc ccattttcca
    14401 ttccctcgac ctggtcaact ggacgcaaat aggccatgtg ctcgacagga aatcccaact
    14461 gataatccac aactgcggca tcagcgcagg catctatgcc cctcagataa tctacaatcc
    14521 ttacaacgat tatttttata tgatcaccac ccaaatttcc gggggtattg gcaatatggt
    14581 cgtcaagacc aaggatcccc accagggatg gagcgaagtg attaaactgg atttcaacgg
    14641 catcgacccc tctctgtttt tcgacgacga cggcaaagct tatgtggtcc acaacgacgg
    14701 ccccgaccgt ggtaaagaac tctacaacgg acacagggta attaaaatct gggaatacga
    14761 taccgagcaa gacaaaatca ttcccggcag cgacaaaatc atcgtggacg gaggcgtgga
    14821 tatatccaaa aagcccattt ggattgaagc tcctcatatt tacaaaaaaa acaacaagta
    14881 ctacctgatg tgtgccgaag gcggaacagg aggctggcat agcgaagtga tttttgtgag
    14941 tgacagcccc aaaggccctt atattccggc acccggcaat cccatcctga ctcagcgcca
    15001 ttttgccggg gatagggcca ataaagtgga ttgggcaggt catgccgatt tggtacttgg
    15061 ccccgacaat aaaacctatt acggggtctt tttagctgtc cgtcccaacg aaaagaaccg
    15121 ggtgaacacg gggcgcgaaa ccttcatcct tcccgtcgac tggtcgggtg aattccccgt
    15181 ctttgtaaac gggctgcttc cgatggaacc taaactaaag atgcctcagg gtgttaccga
    15241 aaacaacacc ggcaaggaga acttcctccc caacggcaac ttcagcttta gcgacaactt
    15301 cagcgccgac aagccggatt accgctggat aagcctgaga ggaccccgcg aagaatttat
    15361 agagacttca aaacaagggc ttaaaatcaa accttatccc gttaatatca aagaagtcaa
    15421 accgacctcc accctctttc tccggcaaat gcacaacagt ttctccttta cggt
//
LOCUS       DAOSTD010000037        15229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_3993_length_15229_cov_15.923290, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000037 DAOSTD010000000
VERSION     DAOSTD010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15229
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3993_length_15229_cov_15.923290"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..3401
                     /gene="secA"
                     /locus_tag="PLI37_03990"
     CDS             99..3401
                     /gene="secA"
                     /locus_tag="PLI37_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOQ58210.1"
                     /translation="MGFNNILTKLFGNKSQRDLKEINPFVEAIKAAYPAVEALSNDQL
                     RALSSEIRNNIQASVSAERAEIERLKEEIETLELEEREKPYEQIDKIEKDITDKLEKA
                     LNDALPQVFSIVKDTARRFKENEKIVVTANDFDRNLAATHDFVHIEGDKAIYQNHWLA
                     GGNEIVWDMVHYDVQLFGGVVLHQGKIAEMATGEGKTLVATLPVFLNALTGNGVHVVT
                     VNDYLSKRDSEWMGPLYMFHGLSVDCIDKHRPNSEERRQAYLADITFGTNNEFGFDYL
                     RDNMAGSPLDLVQRKHNFAIVDEVDSVLIDDARTPLIISGPVPKGDDQLFEEFRPKVE
                     RVVNVQREMATKLLAESKKLMASEDKKEQEEGSLLLYRTHKAMPKNKALIKYLSEQGI
                     KASMQRTEEFYMQNNNQQMHIVTDPLYFVIDEKNNTIELTDKGIDFITGDSEDSQFFV
                     LPDIAAQLSELENQGLNPEDKQTKKDELLQNFSIKSERIHTVQQLLKAYCLFDRDDEY
                     VVVDNKVKIVDEQTGRIMEGRRYSDGLHQAIEAKERVTVEAATQTFATITLQNYFRMY
                     HKLSGMTGTAETEAGEFWDIYKLDVVVIPTNKPIARNDMDDRLYKTKREKYTAVIEEI
                     IRLVEAGRPVLVGTTSVEISELLSRTLTLRKIKHNVLNAKLHQREAEIVANAGQKGVV
                     TIATNMAGRGTDIKLTGEVRKAGGLAIIGTERHESRRVDRQLRGRSGRQGDPGSSVFY
                     VSLEDDLMRLFGSERISGIMDRMGLEEGELIENSMISKAIERAQKKVEENNFGIRKRL
                     LEYDDVMNNQREVIYTRRRHALMGERIGMHLANTIYDTIKSLVEEAYDQRDFEGLKMD
                     ILKYLAMEVPMDEEHFYDQRAENSVDEIYEAALNLYKRKTEKMASIVNPVIQQVYEKQ
                     AAQYENILIPITDGKRTYNIPCNLKAAAESQSKEVVKTFEKAILLHTIDEAWKENLRE
                     LDELRQSVQNASYEQKDPLLIYKLESYNLFKTMIDNINKKSISILMRGQIPIREPESV
                     RKAETQKRHDYSKYRAQRDSLDGGSPARRAGSDREQRQLEPVRVEKKVGRNDPCPCGS
                     GKKYKNCHGRGL"
     gene            3420..4232
                     /locus_tag="PLI37_03995"
     CDS             3420..4232
                     /locus_tag="PLI37_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ58211.1"
                     /translation="MKQQYFKDKVVVITGASSGIGLACARLFAAQGAKLALAARSLDK
                     LQVIQKELPAAVLAVKTDVSKEEDCRKLIEQTIATYGGIDILVNNAGISMRAVFKDLD
                     LKVLHQLMDVNFWGCVYCTKYALPSLLERKGSVVGVTSIAGFKGLPARTGYSASKFAM
                     NGFLDTLRTEHLYDDLHVMIFAPGFTASNVRFSALTADGSPQGETPRKEEKMMSAETV
                     ARHMLRGLRKRKDQMILTPLGKAAVLVNKFFPKLVSRMEYKMMKAEPDSPLS"
     gene            complement(4261..5286)
                     /gene="tdh"
                     /locus_tag="PLI37_04000"
     CDS             complement(4261..5286)
                     /gene="tdh"
                     /locus_tag="PLI37_04000"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOQ58212.1"
                     /translation="MKALVKLRPEKGIWMCERPIPTPGINEVLVKINKTSICGTDLHI
                     YKWDEWSQRTIKTPMIIGHEYAGEIVEMGKGVENLKVGDRVTGEGHIACGHCRNCRRG
                     KLHVCENVVGIGVNRDGAFAEYLAVPASNIVRLDPRISDEMASIMDPFGNATHAALSF
                     PLTGEDVLITGAGLIGSMATAVCRFAGARYIVVSDVIEYRLELARKMGATLTVNPAKG
                     ESIEAAIKSLRMRGFDIGLEMSGSPQAFESMIENMYNGSKIALLGILPDSTQVNWNQI
                     IFKALTLKGIYGREMWETWYQMEQMLITGIDLSPVITHRFDVDDFQKAFDVMESAACG
                     KVILNWA"
     gene            complement(5348..6535)
                     /gene="kbl"
                     /locus_tag="PLI37_04005"
     CDS             complement(5348..6535)
                     /gene="kbl"
                     /locus_tag="PLI37_04005"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOQ58213.1"
                     /translation="MYEKFKEFLSNELESIRASGLYKDERVIHSPQAANIKLADNREV
                     LNFCANNYLGLSNHPLVREAAARVLEERGYGMSSVRFICGTQDLHKELEKEISRFFGT
                     EDSILYASCFDANGGLFEPLLSEQDAIISDALNHASIIDGVRLCKAKRYRYANADVED
                     LEKQLKEAQSQRFRLIVSDGVFSMDGNVAPLDKIYALAQQYDAMLMLDESHSAGVVGQ
                     TGRGITELFGLKGRPEIITGTLGKAFGGAIGGFTTGRREIIQMLRQRSRPYLFSNSIP
                     PMVAAAGIIVFRMLQNTNELQDKLHRNTAYFVQKMSALGFDLKPTESAICAVMLYDAR
                     LSQEFADRMLDEGIYVIGFSYPVVPREQARIRVQLSAEHETEHLDRCIAAFAKVGKAL
                     DVI"
     gene            complement(6670..7236)
                     /locus_tag="PLI37_04010"
     CDS             complement(6670..7236)
                     /locus_tag="PLI37_04010"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOQ58214.1"
                     /translation="MRQSFKKIEKAIKRWYLMLVVGVIFIVLGIWTLATPVDAYIALS
                     FVFAMGFLVSGITEMVFALSNKHQNWGWSLVLGILSTVVGVLLLINPAASMVTLPLYV
                     GFTLLFRSISGMTTAYDMKQYGILDWGTLMVTAVLGLILSFVLLWNPGFASINVVIWT
                     AVAFIVLGGYSIYFSFELRKLNKLMKEE"
     gene            complement(7259..9127)
                     /gene="ftsH"
                     /locus_tag="PLI37_04015"
     CDS             complement(7259..9127)
                     /gene="ftsH"
                     /locus_tag="PLI37_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOQ58215.1"
                     /translation="MDKNKITDDPSQRPNNNWKSFLWTLALLLILNSLVFPNLFKPKI
                     IPTTYSAFINKVDSGLIKQVNIDGNKIYFATPGDTIDRKGDTIDLIFQTGKMNDANLV
                     DRLLVADSPNENGKIEFTETIPRENSPILSFLLYWVLPGAIFFFIWRNAMKSMGGRMG
                     GNYMSFGKNKEKIYAEDEIKTTFADVAGQDEAKESLNEIIDFLHNPKKYNEMGAALPK
                     GVLLVGPPGTGKTLIARAVAGEAKVPFFALSGSEFVQMFVGMGAAKVRDLFSQANQKA
                     PCIIFIDEIDAIGKRRDGMTSNDEREQTLNQLLTEMDGFDGQKGVVILAATNRPESLD
                     KALLRPGRFDRRVQMELPDLEGRKAILQVHLKKVKHEEIDLDLIARATSGASGAELAN
                     IVNEAALRAVRNESSSVTTADLEESIETVIAGAQRKGKVISDEEKRIIAYHEIGHAMV
                     AAMQSHSAPVHKITIIPRTSGALGYTMQIDEGERNLYSKEDLENRIATFTGGRAAEEV
                     IFNRITTGASNDIEQATKIARAMITRYGMSEEFDMVALETINNPYLSSDTSLSCSPET
                     AARIDDVVLSIVKNAHEKAKKIIRENEAKMHELSEYLIKKETITGEEFMKILEAKK"
     gene            complement(9315..10199)
                     /locus_tag="PLI37_04020"
     CDS             complement(9315..10199)
                     /locus_tag="PLI37_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ58216.1"
                     /translation="MMNHSRNPYSTLRESIINLGYEWQEKDYFFCHGLLPKIDNTITD
                     MKEYVGVLLCTDGDIDISINKVDYNLTASTLLIAHPLSIVHIIRSKRQYKGILLFVAR
                     DFLAKNIVNAQLAKPFRQISHHQYTLTNLNREQQKILIDIYKLIYRKRATKQSVFQLE
                     TLRNLFLVFICEVAEIFLESKEVESHSTRNEEIADAFFNLLLRPVKITNKTAFFADQL
                     NISPKHLIKAVKTVAGKAPGVFIHEKITDDAKILLTDKKLTIGQVSEKLGFSETSSFS
                     KFFKKQTGTSPSLFREQF"
     gene            complement(10208..10771)
                     /locus_tag="PLI37_04025"
     CDS             complement(10208..10771)
                     /locus_tag="PLI37_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOQ58217.1"
                     /translation="MTELVEQYPYLLPLLIFFGRIFDVTLGTLRIIFVSKGKRTIAPA
                     IGFFEVFIWIVIISQILSHANDLISYLSYAAGYAAGTYAGMLIESKLAFGVLLYRVYT
                     KESGAELVQMLSKDGFGATMIHGEGSVGKVDVVETVVARQQMKKVERVINNFDEHAFY
                     VTEDVLTAQNGIFPKTANVFKRWRPGK"
     gene            complement(10905..14078)
                     /locus_tag="PLI37_04030"
     CDS             complement(10905..14078)
                     /locus_tag="PLI37_04030"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOQ58218.1"
                     /translation="MKADAMRYIKTLCYICLCSLFVLLSSLPLQAQKALYSDQFPLQD
                     VRLLDGPFKRAFDLNVQNLLKYDMPRLLQPYYKQAGLDNGAQAFGNWAGLDGHVGGHY
                     LSALSIHYAASQDPYTRGLLKARIDSMIACLKTCQEAAVQLDPKMEHYLGGVPDSKNL
                     WTRFAQGDFSIYNRTWVAWYNVHKMFAGLRDAWLYAGDSTALEMFFNYCDWALAITSG
                     LSDAQMQTMLDIEHGGMNEVLADAYRLSGDLKYLNAAKRFTHRLFLDGMANENARFLD
                     NRHANTQVPKFVGIQRIYELSPSLLNYAKAAEFFWKDVVYNRSLALGGNSRSEHFIAP
                     SACGDYVTYREGPETCNTYNMLKLTENLFRLNQKAAYADFYEQALYNHILSTQHPEHG
                     GYVYFTSARPRHYRVYSQVNQAMWCCVGSGMENHGKYNQFVYTHRGDSLYVNLFIPTE
                     LQWRDKGVVITQETAFPYETSSRLSIRIPGGGSRDFSLWLRHPYWADSSAYCLRINGE
                     IVAHNSRPQTYVCISRSWQHGDVLSIDLPMKVHYKTLPNVNEYLALMYGPILLGANVS
                     TDRLDGLLAAEGRMDHVAEGPLLPLNQAPVLVGNRDSLAQAVHLVDSATLSFEVTDFL
                     KHNYDTTYKHLKLQPFYSLHDARYMMYWMQLRPDEYREIYDLLNREEQERLALEARTV
                     DQIATGEQQHDQNHHVQGVNTGVGVYQNTYYREATQGGWFGYEMSTLGVDTLVLRATY
                     WGYESGNRTFDILVEGDTIARENLVGKWNISEFRSVDYEIPVALTAGKSSIRVTFDAL
                     EGHVAGGLYHLRLMSDPGRAGRLRYEQVLVADIDAGNAASESKYRVFVSGSSTGSHQG
                     ETWRDAYNGGYIRYTIPLDGQDSMFLRVRYWGNEGLSREFDMLIDEVAFASESLVGKW
                     NVSAFQEVDYNIPSHLFKGKSKINLKFRARPGNYAGGLFYVWLYALQAQEPDALAYER
                     EDSREMDVLITDGMLRLRLPLEDRGLNNSLRIFGMNGSLMYSGTIREEVRDEVQVFDL
                     RRLPHPQCYVLLCSNEKGSKAQKLMIP"
     gene            complement(14149..>15229)
                     /locus_tag="PLI37_04035"
     CDS             complement(14149..>15229)
                     /locus_tag="PLI37_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOQ58219.1"
                     /translation="RDSVLRVKQLMNYYRYNLSVNRDAIDDPTLVPLQEEFSFIADPN
                     QHKSIEIAFYRGGIIEGYVEILRDGEYVGQSGLRLNVRSDDADFQTVVRTMNDGSFYA
                     MDLHPGKYTLEVDSTQLGFLNVCSEPAQLHFEIRALSEGDYLEGLKLTLIPLEESEAE
                     EPEVVEAEAEVVEAEAEEVEAEEVEVVEMEAVETVEPVETPKAVEAATKIEKVDSGKT
                     AAETAADTPEPKTTQELSAEQEKLFSSGVTRERRGVIAFTFASAELTKDSAPAVLWMA
                     NNLKSGEWKALTIYGNADSTGEEAYNMILSIRRADAVKEALVRQGVSELRIRTVGNGE
                     NKPFATNETKEGRRLNRRIDLEIVE"
BASE COUNT         4091 a         3865 c         3419 g         3854 t
ORIGIN      
        1 gttgtatatt cgtccgccca ccgcatccac aggacaggtt ttattttaaa tactacatca
       61 aaaatcatca gactaaacaa tactaacaat ataccattat gggattcaac aatatattaa
      121 ccaaactttt cggcaataaa tcgcaacgcg atttaaagga gatcaacccc tttgtcgaag
      181 caatcaaagc tgcttaccct gccgtcgaag ctctaagcaa cgaccaactc agggctctga
      241 gcagtgaaat acgcaacaac atccaagcat cggtttctgc cgaaagagca gagatcgaac
      301 gcctcaagga ggaaatcgaa acactggaac tcgaagaacg cgaaaaacct tacgaacaaa
      361 tcgacaaaat agaaaaggac attacggaca aactcgaaaa agctctgaac gatgcattac
      421 cacaggtctt ttcgatcgta aaggataccg cccggcgttt caaggaaaac gaaaaaatcg
      481 tggtcaccgc caacgatttc gaccgcaacc tggctgccac tcacgacttt gtgcatatcg
      541 aaggtgataa agccatctac caaaaccact ggttagccgg cggcaacgaa atagtctggg
      601 atatggtaca ttacgacgta caactctttg gcggtgtagt actgcatcaa ggtaaaattg
      661 ccgagatggc caccggcgaa ggtaaaaccc tggtcgccac cctgcccgtc tttctgaatg
      721 ccctcaccgg caacggcgtc cacgtggtaa cggtgaacga ttatctttcc aagcgtgact
      781 ccgagtggat gggaccgctc tatatgtttc acggcttgtc ggtagattgc atagacaaac
      841 accgccccaa ttccgaagaa cggcgccaag cttatctggc cgacatcact tttggcacca
      901 acaacgaatt cggtttcgac tacctgcgcg acaatatggc cggcagcccg ctcgacctgg
      961 tgcaacgcaa acacaatttc gccattgtgg acgaagtcga ctccgttttg atcgacgatg
     1021 cccgtacccc tctcatcatt tcgggtccgg tgcccaaggg cgacgaccaa ctcttcgaag
     1081 aatttcgtcc caaggtagag cgtgtagtca acgttcagcg cgaaatggcc acaaaattgc
     1141 tggctgagtc caaaaaactc atggcctcgg aagacaaaaa agaacaggaa gaaggctctt
     1201 tgctgctcta ccgcactcac aaggccatgc ccaaaaacaa agctctgatc aaatacctca
     1261 gcgagcaggg catcaaagcc agtatgcaaa gaaccgagga gttctatatg caaaacaaca
     1321 accaacaaat gcatattgtg accgaccccc tgtattttgt catcgacgaa aaaaacaata
     1381 ccatcgagct caccgacaaa gggatcgatt tcatcaccgg cgacagcgaa gacagccagt
     1441 tctttgtttt gcccgacata gcggcacaac tgtcggaact cgaaaaccag gggctgaatc
     1501 cggaagacaa gcagacaaaa aaagacgaac tgctgcaaaa cttctccatc aaatcggaac
     1561 gcattcacac ggttcagcaa ttgctcaaag cctattgtct cttcgaccgt gacgacgaat
     1621 atgtggtagt cgacaacaaa gtgaaaattg ttgacgaaca aaccggccgt attatggaag
     1681 gccgccgcta ttccgacggt ttacaccagg ccatcgaggc caaggaaaga gttaccgtcg
     1741 aagcggccac tcagaccttt gccaccatca cgctgcagaa ttatttcaga atgtaccata
     1801 agctgtcggg tatgaccggt actgccgaaa ccgaggcagg tgaattctgg gatatttata
     1861 aactggatgt agtggtgatt ccgaccaaca agcccatcgc ccgtaacgat atggacgacc
     1921 gcctctacaa gaccaagaga gagaagtaca cggccgttat cgaagaaatc atccgcttgg
     1981 tcgaagccgg gcgaccggtg ctggtaggta ctacttcggt agaaatctcc gaattgttga
     2041 gccgtacact caccctacgt aaaatcaagc ataacgtgct caacgccaag ctacaccagc
     2101 gcgaagccga aatcgtggcc aacgccggac aaaaaggcgt ggtaaccatt gccaccaaca
     2161 tggcgggacg tggtaccgac atcaagctca ccggagaagt tagaaaggcc ggaggtttgg
     2221 ccatcatagg cacggaacgt cacgaatcgc gccgtgtcga ccgtcagctg cgcggacgtt
     2281 caggccgtca gggcgacccc ggatcttcgg tgttttacgt atcgctcgaa gacgacctga
     2341 tgcgtctgtt cggatcggaa cgtatttcgg gcatcatgga ccgcatggga ctggaagaag
     2401 gcgaactcat cgaaaactcc atgatttcta aagccatcga aagggcacag aaaaaagtgg
     2461 aagaaaacaa cttcggcata cgtaaacgtc tgctcgaata cgacgacgtt atgaacaacc
     2521 agcgcgaagt catctacacc aggcgccgcc atgccctgat gggagaacgt atcggtatgc
     2581 acctggccaa caccatttac gacaccatca aaagtttagt cgaagaagct tacgaccaac
     2641 gcgacttcga gggtctcaaa atggacatac tcaaatacct ggctatggaa gtgcccatgg
     2701 acgaagagca tttttacgat cagagagccg aaaacagcgt agacgaaata tacgaagcgg
     2761 ccctgaacct ctacaaacgt aaaaccgaaa agatggcctc catagtcaat ccggtcattc
     2821 agcaggtcta cgaaaagcag gctgcccaat acgaaaacat tctgataccc atcaccgatg
     2881 gtaaacgtac ttacaacatc ccctgtaacc tgaaagcagc cgccgaaagc cagtcgaaag
     2941 aagtggtaaa aacgttcgaa aaagccattc tgttgcatac catcgacgaa gcctggaaag
     3001 aaaatctccg cgagctcgac gagttgcgtc aatcggtaca aaatgccagc tacgaacaaa
     3061 aagaccctct gcttatctac aaactggagt cgtacaacct cttcaaaacc atgatcgaca
     3121 atatcaataa gaagagtatc tctattttga tgcgcggaca aattcctata cgcgaacccg
     3181 aatcggtacg caaagccgaa acgcaaaaac gccacgacta cagtaaatac cgtgctcaaa
     3241 gagactcttt ggatggcgga agtccggctc gccgagctgg gtcagacaga gagcaacgcc
     3301 agctggaacc ggtaagggta gaaaaaaagg tgggtcgcaa cgatccctgt ccctgcggtt
     3361 cgggcaaaaa gtacaaaaat tgccacggta ggggtttata aacatttaag actgaggtta
     3421 tgaaacaaca gtattttaaa gacaaagttg tagtgatcac cggagcgtcg tcgggcatag
     3481 gcctggcttg tgcccggctt tttgccgctc agggtgcaaa actcgcgctg gcagcaagat
     3541 ccctcgacaa actacaagtc atacagaagg aacttcctgc agccgtactg gccgttaaaa
     3601 ccgacgtcag caaagaagaa gattgccgaa aactcataga gcaaaccata gcaacctatg
     3661 gaggcatcga tatcctggta aacaatgccg gtatttccat gcgggccgtt ttcaaagacc
     3721 tcgacttgaa agtcttgcat caattgatgg atgtaaattt ctggggctgt gtctattgca
     3781 ccaaatacgc tttgcccagc ctgctcgaac gcaagggctc ggtggtgggc gttacttcca
     3841 tagccggctt caagggactg cccgcccgca caggctattc ggcctcaaaa tttgccatga
     3901 acggctttct cgacaccctg cgtaccgaac atctgtacga cgacttgcat gtgatgattt
     3961 ttgccccggg ctttacagcc tccaatgtgc gcttttcggc tctgacagcc gatggcagcc
     4021 ctcagggcga gactccccgc aaggaagaaa aaatgatgtc ggccgaaacc gtggcccgtc
     4081 atatgctacg gggactgcgc aaacgcaaag atcagatgat actcactccc ctgggtaaag
     4141 cggcagtgct ggtgaacaag tttttcccga agctcgtcag ccgcatggaa tacaaaatga
     4201 tgaaagccga gcccgactct cctttgtctt aaacgagtgt tcaggcccag ttcaaagtgt
     4261 tcaggcccag ttcaaaataa ctttgccgca ggcggcagac tccattacat cgaaggcctt
     4321 ttgaaaatcg tccacatcaa aacggtgtgt aataaccggg ctcaaatcga tgcctgtgat
     4381 aagcatttgc tccatctgat accaggtttc ccacatttcg cggccataaa taccctttaa
     4441 ggtcaaagcc ttgaatatga tttggttcca gtttacctga gtagaatcgg gcaaaatacc
     4501 caaaagagca atcttcgatc cgttgtacat attttcgatc atgctttcga aggcctgcgg
     4561 agaacccgac atttccaggc ctatatcgaa accacgcatg cgcagcgact taatcgcagc
     4621 ttcgatgctt tcgcctttgg caggattcac tgtgagggtg gcgcccattt tccgagccag
     4681 ctccaaacgg tattcgataa catcgctcac cactatgtaa cgggctccgg caaagcggca
     4741 aacagccgtt gccatggagc cgatcaggcc ggctccggtg ataagcacgt cttcgcctgt
     4801 caggggaaac gacaaagccg catgtgttgc gttaccgaaa ggatccatga tcgatgccat
     4861 ctcgtcgctg atgcgggggt cgagtcttac gatgttcgaa gccggaaccg ccaggtattc
     4921 ggcaaaggcc ccatcgcgat tcaccccaat accgaccacg ttttcgcaga catgcagctt
     4981 accccggcgg caattgcggc aatgtccgca ggcgatgtgt ccttcgccgg tcacccggtc
     5041 gccgactttt aaattttcca cacccttacc catttccaca atttctcctg cgtactcgtg
     5101 tccgatgatc atgggtgtct tgatagtacg ctgcgaccat tcgtcccatt tgtagatatg
     5161 cagatcggtg ccgcaaatgg aggttttgtt gattttgacc agcacctcgt tgataccggg
     5221 cgtgggaatg ggtctctcac acatccagat gcctttttcg gggcgcagtt ttaccaaagc
     5281 tttcatctta gaaatctgtt aaataaaacc aataagctct tatagttcaa cgctctagtt
     5341 gaaattatca tatcacatcc agagccttgc ccactttggc aaaggcagca atgcaacgat
     5401 ccaagtgttc ggtttcatgt tcggccgaga gctgaacacg aatacgggcc tgctctctgg
     5461 gcaccaccgg ataagagaac cctatcacat aaatgccttc gtcgagcata cggtcggcaa
     5521 attcctgcga aagcctggca tcgtaaagca tcacggcgca tatggccgac tccgtgggtt
     5581 ttaaatcgaa gcccaaagca gacattttct gcacaaaata ggccgtgttg cgatgtagtt
     5641 tatcctggag ttcgttggtg ttttgcagca ttctgaacac tatgataccg gcagcggcca
     5701 ccatgggcgg aatggaattg gagaacaggt aaggtctgga tcgctgacgc aacatctgaa
     5761 taatttcgcg acgcccggtg gtgaaaccgc ctatggcccc tccaaaagct ttacccaagg
     5821 tgcctgtaat gatttcaggc cggcctttga gcccaaacaa ttcggttatt cccctgcccg
     5881 tttgcccgac cactcctgcc gaatgcgatt catccagcat cagcatagca tcgtattgct
     5941 gagccagggc gtagatttta tccaatggag ccacgttgcc gtccatcgaa aaaactccat
     6001 cgcttacaat cagcctaaaa cgctgagact gtgcttcctt caattgcttt tccaaatcct
     6061 ccacatcggc attggcgtaa cggtagcgtt tggccttgca aagcctgacc ccgtcgataa
     6121 tggaagcatg gttgagcgca tcggaaataa tggcgtcctg ctcactcagc aaaggttcaa
     6181 acaaacctcc gttggcatca aaacaagagg cgtagagaat actatcttcc gtaccgaaga
     6241 agcgggagat ttccttttcc aattctttgt gcaaatcctg ggtgccgcaa ataaaacgca
     6301 ccgacgacat gccgtatcct ctctcttcga gcacacgggc cgctgcttcc ctcaccagag
     6361 gatggttgga caatcccagg taattattgg cgcaaaaatt caacacttca cggttatcgg
     6421 ctaattttat gttggccgcc tgaggagagt gaatcaccct ttcgtcctta tacaatcccg
     6481 aagcacgaat gctttcgagt tcattactga gaaattcttt aaatttttcg tacatattat
     6541 tgtgtttttg tttaattcta cccctatttg ccacaaattt attcataatt cttgacaaac
     6601 aaacacaaca gcgggcacgc ttaaaaaaaa gcgcacccgc acgaacacct atgaaagatc
     6661 attgaaattt tattcctctt tcatcaattt attcagcttt cgcaattcaa aggagaaata
     6721 aatggaataa cctcctaaga cgatgaatgc tacagcagtc caaataacca cgttgatact
     6781 tgcgaacccc ggattccaaa gcaaaacaaa agataaaatc aagcccaata cggcggttac
     6841 cattaaggta ccccaatcta aaataccgta ttgcttcatg tcgtaagccg tggtcatgcc
     6901 cgatatggag cggaaaagca aggtgaatcc cacgtaaagc ggtaaagtta ccatagacgc
     6961 agccggattg atcagtagaa gaacccccac aacggtactc aatatcccca ataccagcga
     7021 ccaaccccaa ttttgatgtt tgttgcttaa ggcaaaaacc atttcggtaa ttccgctgac
     7081 tagaaatccc atagcaaaca caaaagacaa agctatgtag gcatctaccg gcgtggctag
     7141 tgtccaaata cctaaaacta taaatatcac tcctacaacg agcattaaat accatcgttt
     7201 aatggctttt tctattttct taaatgactg tctcatgatg ttgaatttta tacgttattt
     7261 attttttagc ttccaatatt ttcataaact cttctcctgt aatggtttct tttttaatga
     7321 gatattccga taactcgtgc attttggctt cgttttcgcg aatgattttt ttcgcttttt
     7381 cgtgcgcatt tttcactatg gaaagcacta catcgtcgat acgggctgcc gtttcaggag
     7441 aacaacttaa agatgtgtcg ctgctcaaat agggattgtt tattgtttcc aacgccacca
     7501 tatcaaattc ctcgctcatt ccgtagcggg taatcatggc acgggcaatt tttgtagctt
     7561 gttcaatgtc gttggatgca cctgtggtaa ttcggttaaa aataacctct tcggcggcgc
     7621 gtccaccggt aaatgtggct attctgtttt ccaaatcttc tttactatat aaattacgtt
     7681 caccttcgtc aatttgcatg gtataaccga gggcccccga tgtacgtggg ataatggtaa
     7741 ttttatgcac cggagcggaa tgcgactgca tagcggcaac catagcgtgt ccgatttcgt
     7801 gataagcaat aatgcgtttt tcttcgtcgg aaatcacttt tccttttctc tgtgctccgg
     7861 cgattacggt ttctatgctt tcttccaagt cggcggttgt cacgctgcta ctttcgtttc
     7921 ttacggctct cagagcggct tcattcacaa tattggccag ttcggcaccc gatgcacccg
     7981 aggtggcgcg tgcaatcaaa tccagatcta tttcttcgtg ctttactttt ttcagatgca
     8041 cctgcaagat ggctttacgt ccttccaaat cgggaagttc catttgaacc cgccggtcga
     8101 acctgccggg acggagcaat gctttgtcga gactttcggg gcgattggtc gctgccagaa
     8161 taaccactcc tttttgtccg tcgaaaccgt ccatttccgt gagcaattga ttcaatgttt
     8221 gttcacgctc atcgttggaa gtcattccgt cgcgcctttt gccgatggca tcaatctcat
     8281 cgataaagat gatgcagggt gctttttggt ttgcttgact aaacaaatcg cgcactttgg
     8341 cggcacccat tcccacaaac atttgaacaa attcggatcc ggaaagagca aagaaaggca
     8401 cttttgcttc gcccgcaaca gctcttgcaa tcagtgtttt acctgttccc ggagggccaa
     8461 ccaataaaac acctttgggc aaggcagcac ccatttcgtt atatttcttg gggttatgca
     8521 gaaaatcgat tatttcgttt aaagattctt tggcttcatc ttgccccgcc acatcggcaa
     8581 aggtggtttt tatctcgtct tcggcgtaaa ttttctcttt gtttttcccg aaactcatat
     8641 aatttccgcc cattcttcca cccatcgact tcatggcatt tcgccagata aagaagaaaa
     8701 tcgctcccgg caatacccag taaagcaaga aactcaatat gggggaattt tcgcggggaa
     8761 tggtttcggt aaattctatt tttccgtttt cgttggggct gtctgccact aataaacggt
     8821 caaccaaatt ggcatcgttc atttttcctg tttgaaaaat caagtcgatg gtgtcgccct
     8881 tacggtcgat ggtgtcgccg ggtgtagcaa aataaatttt gtttccatca atgtttacct
     8941 gcttgattaa tccggaatcg actttattga taaaagcgct gtacgttgtc ggaataattt
     9001 taggtttgaa cagatttgga aaaaccaaag aattaagtat cagcaataag gcaagtgtcc
     9061 acaaaaaact tttccaattg ttattgggtc tttgcgaagg atcgtcggtg attttattct
     9121 tatccatttc tataaattat aatgctttgc tttgtaatgt tttccggggc tgtctcaaaa
     9181 cacatatcaa aaacacagca gttgagcact tacgagacaa gtcctgaata atgcgaatta
     9241 tgacccacaa aattaagaaa cacagcaggc atttcgttgt ccgaaattca catgcagttg
     9301 cctaaaattc tcttttagaa ctgctctctg aataaggatg gagaggtgcc tgtttgcttt
     9361 ttaaaaaatt tgctgaacga cgaggtttcg gaaaatccca atttttctga aacctggccg
     9421 atagtaagct ttttatcggt caacaatatc ttggcatcgt cggtaatttt ctcatggata
     9481 aaaacgccgg gagcttttcc ggctactgtt ttaaccgctt tgatcaggtg tttaggggag
     9541 atatttaatt ggtcggcaaa gaaggccgtt ttgttcgtta tttttaccgg cctcaaaagc
     9601 aaattgaaaa aggcatcggc gatttcttcg tttcttgttg aatggctttc cacttctttg
     9661 ctttccaaga atatttcggc tacttcgcaa ataaaaacca agaacaaatt gcgcaaggtt
     9721 tcaagttgaa acaccgattg tttggttgcc ctttttctgt aaatcaattt gtaaatatcg
     9781 atcagaatct tttgctgttc tctgttaagg ttggtaagtg tgtattggtg atggctgatt
     9841 tgacgaaaag gttttgcaag ttgagcattt acaatgtttt tagccaaaaa gtctcgcgcc
     9901 acaaacaaaa gaatgccttt gtattgtctt ttcgatcgta taatatgtac aatggacaaa
     9961 ggatgtgcga taagcaaagt cgatgccgtc aggttataat ctaccttatt gatacttata
    10021 tcaatatcgc catccgtaca caacaacaca cccacatact ctttcatgtc ggtaatcgtg
    10081 ttgtcgattt tcggaagcag accatggcag aaaaaataat ctttttcctg ccattcgtaa
    10141 cccagattta tgatggattc tcgcaatgta gaatatggat tgcgtgagtg attcatcatt
    10201 atttaactta cttccccggt ctccagcgtt taaagacatt ggcggttttg gggaaaatgc
    10261 cgttttgggc tgtaaggaca tcttcggtta cgtagaaggc gtgttcgtcg aaattattta
    10321 tcaccctttc tacctttttc atttgctgcc gtgccactac cgtttctacc acatccacct
    10381 ttccaaccga gccttcgccg tgaatcatgg ttgccccgaa tccgtctttg ctcagcattt
    10441 gtacaagctc ggcgccactc tctttggtgt acactctgta taacaacacg ccgaaggcca
    10501 atttactctc tatcagcata cctgcgtagg taccggcggc gtaaccggcg gcgtaggaaa
    10561 gataagatat caaatcgttg gcatgagaaa gaatctgcga aataatcaca atccatataa
    10621 atacctcgaa gaaaccgata gccggtgcta tcgtcctttt ccctttggag acaaagatta
    10681 ttctcaaggt acccagtgta acgtcaaaaa ttctcccgaa gaaaatcaga aggggcaaca
    10741 aataaggata ttgttctact aattctgtca ttttattttg tcttaaaatc tacttgtact
    10801 cacttggttt gtaaatcgcc tggtttgatc acttgccgga cggactgaat aaccgctgca
    10861 aattcgtatc gtcgtaactg cttattggat agattggtaa agctttaggg gatcatcagc
    10921 ttttgagcct tggaaccttt ttcgttggaa cagagcaaca catagcattg cggatggggc
    10981 agacggcgaa gatcaaacac ctgaacttcg tccctaacct cctcccgaat tgttcctgaa
    11041 tacatcagcg aaccgttcat cccaaaaata cgcaaactgt tgtttaaacc ccggtcttcg
    11101 aggggcagcc tgagtcgaag catgccgtcg gtgattaaaa catccatttc cctgctgtct
    11161 tcacgctcgt aagccagagc gtccggttcc tgagcttgca gggcatagag ccaaacgtaa
    11221 aacaatccgc cggcgtaatt gcccggcctt gccctgaatt tcaggttgat cttgcttttc
    11281 cctttaaaca agtgcgacgg gatgttgtaa tctacttcct gaaaggcact tacattccac
    11341 ttacccacca ggctttcgct ggcaaacgcc acttcgtcta tcaacatatc gaattcgcgg
    11401 ctcaggcctt cgttgcccca gtagcgcaca cgcaaaaaca tactgtcctg gccgtcgagt
    11461 ggaatggtgt aacgaatata tcctccgttg taggcatctc tccaggtctc gccctgatgg
    11521 ctgccggtgc tggaacccga tacgaaaacc ctgtatttgc tttccgaagc ggcattgcca
    11581 gcatcgatgt ccgccaccaa cacttgctcg tagcgcagtc tgcctgccct acccggatcg
    11641 gacatcaggc gaaggtggta caagcctccg gccacatgcc cttccagcgc atcaaaggta
    11701 actctgatac tgcttttgcc tgccgtcagg gctaccggaa tttcataatc caccgatctg
    11761 aactccgaaa tattccattt cccgaccagg ttttcgcggg caatggtatc gccttcgacc
    11821 agaatgtcaa aagttctgtt accgctttcg taaccccaat aagttgctct caacaccagg
    11881 gtatcgacgc ccagagtgct catttcataa ccgaaccagc ctccctgagt ggcttctctg
    11941 taataggtat tttgatacac ccctaccccg gtattcactc cctgaacatg gtgattctgg
    12001 tcgtgttgtt gctcgccggt ggcaatctgg tctaccgtgc gggcttccag agccagacgc
    12061 tcctgctctt cgcggttcag cagatcgtaa atttcccggt attcatcggg gcgcaactgc
    12121 atccagtaca tcatataacg ggcatcgtgc agggaataaa aaggttgcaa cttcaaatgc
    12181 ttgtaggtgg tatcgtagtt atgtttcaaa aaatcagtta cctcaaaact caatgtagcc
    12241 gaatcgacca aatgtacggc ctgagcaagg gaatcgcgat tccctaccaa taccggcgcc
    12301 tgattcaagg gcagcagggg cccttcggcc acgtgatcca tacggccctc ggcggccagc
    12361 aaaccgtcca gccggtcggt gcttacattg gctcccaaca aaatggggcc gtacatcaaa
    12421 gccaggtatt cgttcacatt gggcagagtt ttataatgga ctttcattgg caggtctata
    12481 ctcagcacat cgccgtgttg ccagcttcgg ctgatgcaaa cataagtttg cggacggctg
    12541 ttgtgagcaa cgatttcccc gtttatacgc aagcaatagg cgctgctgtc ggcccaataa
    12601 ggatgacgca gccaaaggct gaaatcacgt gatccgcctc cgggaatccg gatgctcagg
    12661 cggctgcttg tttcataggg aaaagccgtc tcctgggtaa taaccactcc tttgtcgcgc
    12721 cattggagtt ccgtggggat aaacaaattc acgtacaagg aatcgccacg atgagtataa
    12781 acaaactggt tgtatttacc gtgattttcc ataccgctgc ccacgcagca ccacatagcc
    12841 tgattcacct gcgaatacac ccggtaatgc cggggccggg ccgaagtaaa atacacatag
    12901 cccccgtgtt cggggtgttg agtcgataaa atatggttgt agagcgcctg ttcgtaaaaa
    12961 tcggcgtaag ctgccttttg attgaggcga aaaaggtttt cggtcagttt gagcatattg
    13021 taagtattgc aagtttcagg gccttcgcgg taagtgacgt aatcaccgca ggccgagggt
    13081 gcaataaaat gctcgctgcg gctgttgccg cctaaagcca gagaacgatt ataaacaacg
    13141 tctttccaaa aaaattctgc cgccttggcg tagttgagca aggagggcga gagctcataa
    13201 attcgctgga ttccaacaaa tttaggtact tgggtattgg cgtgcctgtt atcgagaaaa
    13261 cgtgcattct cgttggccat gccatcgagg aataatctat gggtaaaccg tttggctgcg
    13321 ttcaaatatt tgaggtcgcc actgagccgg taagcgtcgg ccagcacctc gttcatgcct
    13381 ccatgctcaa tatccagcat ggtctgcatc tgagcgtcag acaatcctga agtaatcgca
    13441 agggcccagt cgcaataatt aaaaaacatt tccaaagcgg tgctgtcgcc cgcatacagc
    13501 caggcatcgc gcaaaccggc aaacatcttg tgcacattat accaggccac ccaagtgcgg
    13561 ttataaatgc tgaaatcgcc ctgagcaaaa cgggtccaga ggtttttgct gtcgggaaca
    13621 cctcccaggt aatgctccat tttgggatcc agttgaacgg cggcctcctg acatgttttc
    13681 aggcaggcaa tcatgctgtc tatcctggct ttgagcaggc ccctggtgta aggatcttgc
    13741 gaggcggcat aatgaatgga cagggccgaa aggtaatgcc cgcccacgtg accgtccaaa
    13801 cccgcccagt tgccgaaagc ctgggctccg ttgtcgagac ctgcttgttt gtaatagggc
    13861 tgcaataaac gaggcatatc gtacttgagc aagttttgaa cattcaggtc aaaggcccgc
    13921 ttaaaagggc cgtcgagcaa acgaacatcc tgcaaaggaa attgatcgga atacaaagct
    13981 ttttgggcct gcagaggcaa agaggaaagg agtacaaata acgagcataa acaaatataa
    14041 cacagcgttt taatataacg catagcgtct gctttcatag aaaatgaatt taaaatttaa
    14101 agaacacaaa tgtaccttca ttccggcgaa tcctcaaaca aaattccatt attccacaat
    14161 ctccagatcg atgcgccggt taagccggcg tccctctttt gtttcgttgg tggcaaaagg
    14221 tttattttcg ccgttgccta cggtgcggat gcgcagttcg gatactcctt gccggactaa
    14281 tgcctctttg acggcatcgg cccgcctgat cgaaagaatc atattgtaag cttcttcgcc
    14341 tgtgctgtcg gcattaccgt aaatggtcag ggctttccac tcaccgctct tgagattatt
    14401 ggccatccaa aggactgcag gagccgaatc tttggttaat tccgccgaag caaaggtgaa
    14461 agcgatcact cctctgcgct cgcgagtgac tccggaacta aagagttttt cctgttcggc
    14521 acttaactct tgggttgttt ttggctccgg tgtatccgca gcggtttcag cagcggtttt
    14581 acctgagtca actttctcaa ttttagtcgc ggcctcaaca gctttagggg tttcaacagg
    14641 ctcaacggtt tcgacagcct ccatctcaac aacttcaact tcctcagctt caacctcctc
    14701 ggcctcagct tcaacaacct ctgcttcagc ttcaacaacc tctggctcct cggcttcgct
    14761 ttcctccagc ggaatcaacg tcagtttcag cccttcgagg taatcgcctt cggagagggc
    14821 tctgatttca aaatgcaatt gagccggttc gctgcacaca ttcaaaaagc ccaattgcgt
    14881 agaatctact tcgagcgtgt atttgcccgg atgcaggtcc atagcataga aactaccatc
    14941 gttcatggtt ctcaccaccg tttgaaaatc ggcgtcatcc gaacgcacat tcaatcgcag
    15001 accactttgt cccacatact cgccatctct cagtatctcc acataacctt caataatgcc
    15061 gcccctgtag aaagcaattt ctatcgactt gtgctggttg ggatcggcaa taaaggagaa
    15121 ttcctcctgc agaggcacca gggtaggatc gtcgatagca tcgcgattca cgctgagatt
    15181 gtagcgatag taattcatca attgcttaac acgcaacacg ctgtcgcgg
//
LOCUS       DAOSTD010000038        15207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_4000_length_15207_cov_14.921067, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000038 DAOSTD010000000
VERSION     DAOSTD010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15207
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4000_length_15207_cov_14.921067"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..786)
                     /locus_tag="PLI37_04040"
     CDS             complement(115..786)
                     /locus_tag="PLI37_04040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOQ58220.1"
                     /translation="MNYTLIRSRRKTLTLQIKPDGSLLVRCPLRLNLREVERFIQAKQ
                     HWIVAKQQLLRENFEKRQAFRLHPGMELRYLGRTYPLRLSENRKSGFDARGFYLGNKI
                     PEEKIRATVVKIYKELATTHITAKVEHYARLYKLKYTDVRIGSAKTRWGSCSSKNRLI
                     FSWRLIMADELCVDYVVLHELAHTLEHNHSHQFWTKLGRMMPNYPEARRQLRIFGRQL
                     QAEDW"
     gene            complement(783..1622)
                     /gene="nadC"
                     /locus_tag="PLI37_04045"
     CDS             complement(783..1622)
                     /gene="nadC"
                     /locus_tag="PLI37_04045"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOQ58221.1"
                     /translation="MASLEDFIRNAFAEDIGEGDHTSLSCIPASARGKSMLLIKEAGV
                     VAGVRVALEVFRLFDPQLQTTVFIQDGQKVKPGDIVFETAGSVRSLLQTERLMLNIMQ
                     RMSGIATTTRLYADRLQGLHTKVIDTRKTTPGMRLLEKEAVVLGGGSNHRMGLYDMIL
                     LKDNHVDFAGGIAKAIAATRDYLQEKKLNLKIEIEVRSLDELEQVLQCGGVDRIMLDN
                     FDPATTRKAVERIAGRYETESSGGITLDTIRAYAECGVDFISVGALTHSIRSLDMSFK
                     AVL"
     gene            1727..2200
                     /gene="rlmH"
                     /locus_tag="PLI37_04050"
     CDS             1727..2200
                     /gene="rlmH"
                     /locus_tag="PLI37_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOQ58222.1"
                     /translation="MKVILLLSGKTGEDYARKAIKLFEERLKHYLAFEIVYLPELKRS
                     GKISEQEQKQREAALLEPWIKPENTLVLLDERGREYTSEAFAAYLQKKMNAGLRNLVF
                     VCGGPYGFDASVYKRVPERLSLSKMTFSHQMVRVFFVEQLYRAMTILRGEPYHHS"
     assembly_gap    2223..2322
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2764..4596
                     /gene="ilvD"
                     /locus_tag="PLI37_04055"
     CDS             2764..4596
                     /gene="ilvD"
                     /locus_tag="PLI37_04055"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOQ58223.1"
                     /translation="MKHPLRSAVCTEGRRMAGARALWLANGMKKEQLGKPIIAIVNSF
                     TQFVPGHVHLHNIGQEIKAEIEKAGCFAAEFNTIAIDDGIAMGHDGMLYSLPSRDLIA
                     DSVEYMVNAHKADAMICISNCDKITPGMLMAALRMNIPSVFVSGGPMEAGKLGDTYLD
                     LIDAMVQAADPKVSDEELAAVEAVACPGCGSCSGMFTANSMNCLNEALGMALPGNGTV
                     LATHVNRKKIFQQAARQIVHNTFAYYRDGDQRVLPRSIASKAAFMNAMALDIAMGGST
                     NTILHLLALAQEAEVDFTMDDIDALSRKIPCLCKVAPSSHYHVQDVNRAGGILSIMDE
                     LDQAALIDNSVYRVDGYTLKEAMDKYSIRSANVLPEALALYKSAPGGKFNNTLASQGA
                     VYKELDTDRSLGCIRDIEHAYTRDGGLAILKGNIAQDGCVVKTAGVDESIWKFSGKAK
                     VFHSQEAACDGILDGRVEAGDVVVIVFEGPKGGPGMQEMLYPTSYIKSRGLGKSCALI
                     TDGRFSGGTSGLSIGHISPEAASGGAVALIRDNDIIDIDIPHRSINLRIGAEELNQRW
                     LEEEKKAAGAFKPKRDRKVSKALKAYAGMVSSADKGAVRIIEED"
     gene            4600..6303
                     /gene="ilvB"
                     /locus_tag="PLI37_04060"
     CDS             4600..6303
                     /gene="ilvB"
                     /locus_tag="PLI37_04060"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOQ58224.1"
                     /translation="MEKKKITGAEALLFAFKEEKVDTIFGYPGGAIIPVFDALYTHKE
                     RLAFHHILTRHEQGAAHAAQGYARVSGKVGVVIVTSGPAAANIVTGVADAMLDSTPLV
                     VVTGQTSVAALGSDAFQETDVLGITQAITKWSYQIRRAEDISWAVARAFYIAREGRPG
                     PVVLDLSKDAQNQLIEFSYEKCRYIRSYQPEPELNPDKIARAAQLINQAKKPFAVVGQ
                     GVLLGRAEQELFNFLNKADIPAASSLLGLDVMPVDYRLNMGMVGMHGNVAPNKKTNEC
                     DVLIAIGMRFSDRVTGKLSEYAPQAKVIHFDIDPSEINKNVQVEVGVLGNVKKSLPAV
                     TELLDKADHKEWIESFEEHKLCEYREVVSKDLYPNNGILTMAEVVKCLSDNTGENTIV
                     VTDVGQNQITAARYFSSYNTRKIVTSGGLGTMGFGLPAGVGAALGSPESKVFVISGDG
                     GIQMTIQELGTIMEYKIPVKVIILNNNYLGMVRQWQELFYEERYSSTSIQNPDFIALA
                     AAYGIKGRKVEQRKDLAEAVREMIAADSAYVLEVMVEKKGIVYPMVPAGKCITEIMLK
                     S"
     gene            6305..6865
                     /gene="ilvN"
                     /locus_tag="PLI37_04065"
     CDS             6305..6865
                     /gene="ilvN"
                     /locus_tag="PLI37_04065"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOQ58225.1"
                     /translation="MMEKTLYTVTVYSENQVGLLNQVTIVFTRRQVNIESLTVSRSAI
                     PGVHKFTITANTDRETIRKIVLQIEKRIDVLKAYYYTDDEIIFQEIALYKVPTLSLMS
                     SNGIETLVRKYNARILEVTKDYTVIEQAGHKAEIDAMFDALNKNKILQFVRSGRVAIT
                     RSTVERLSNFLEEMEARRKEVFNGNN"
     gene            6890..7936
                     /gene="ilvC"
                     /locus_tag="PLI37_04070"
     CDS             6890..7936
                     /gene="ilvC"
                     /locus_tag="PLI37_04070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOQ58226.1"
                     /translation="MAKMNFGGVMENVITREEFPLKKAKETLKNETIAVIGYGVQGPG
                     QSKNLRDNGFNVIVGQRKGGATWDKAVADGWVPGETLFEIEEACRRGTIIQYLLSDAA
                     QIEVWPRIKPHLTAGKALYFSHGFAITYKDRTNIIPPKDIDVILVAPKGSGTSLRTMF
                     LEGRGLNSSYAIFQDATGKAFDRVVALGIGVGSGYLFETTFQREVYSDLTGERGTLMG
                     AIQGLLLAQYEVLRANGHTPSEAFNETVEELTQSLMPLFAQKGMDWMYANCSTTAQRG
                     ALDWMGPFHDAVKPVFEKLYKEVANGNEAQRSIDSNSKPDYREKLEEELRQLRESEMW
                     QTGAVVRKLRPENN"
     gene            complement(7933..9126)
                     /locus_tag="PLI37_04075"
     CDS             complement(7933..9126)
                     /locus_tag="PLI37_04075"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOQ58227.1"
                     /translation="MNPVSLRLASLAPSETLAMSQKSNELKAMGFDVINMSVGEPDFF
                     TPDHIKLAAKQAVDNNFSFYSPVPGYPALRKAIVEKLKKENGLDYTVDQIVCSNGAKQ
                     SVCNVLLSIISPGDEVIVPAPYWVSYVEMVKLAEGKNVIVSAGIEQDFKITAAQLEAA
                     VTPKTKALILCSPSNPTGSVYSKEELQALAAVLAKHPQVYILSDEIYEHINYLGKHES
                     IAQFESVRERVIVINGVSKAYAMTGWRIGWIAAPQWIASACNKLQGQYTSGPCSVSQE
                     AARAAYTGDQACVAEMRTAFERRKNLIVKLMREVPGFKVQEPKGAFYVFPDCSAYIGK
                     SFKDKTITNATDLAMFLLEEAHVAAVGGVAFGAPNCLRFSYATSDENIVKAIDRIKVA
                     LAKLA"
     gene            complement(9174..10181)
                     /locus_tag="PLI37_04080"
     CDS             complement(9174..10181)
                     /locus_tag="PLI37_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOQ58228.1"
                     /translation="MKKRTALLILLTVFLCGTWDVQAKSKEKTDKKSFWDLIERPKKA
                     YLFSYFMGGGDGLHFLYSYDGILWKNLNRGEIFLKPEVGTAKLMRDPSIVQAPDGVFH
                     MVWTTGWTENNIGYASSTDLIHWSEQREIPVMAHEPEVRNTWAPELFYDKKSKQYYII
                     WASTIPGRFPAPGSSESDYNHRLYYTTTKDFERFSPTELFYDPEFNVIDAAILKHKGI
                     YYMFLKNETLEPVEKNIRVVASKKLTAFPKTVSEPISGIEWAEGPTPLQIGKYTYVYW
                     DKYRNQRYGAARCKDLKKGVWDDISKIISFPSGVRHGTAFEVEQEVLNNLLKMDENPQ
                     N"
     gene            complement(10189..11406)
                     /locus_tag="PLI37_04085"
     CDS             complement(10189..11406)
                     /locus_tag="PLI37_04085"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOQ58229.1"
                     /translation="MQEKIKLNSIDEAIEAIRQGEIIIVVDDEDRENEGDFITSAELI
                     TPEKVNFMITVGRGVLCAPITEERCLELELDKQVANNTSILGTPFTVTVDKLEGCTTG
                     VSSHDRAETIKALADPNTKPEDLGRPGHISPLYAKNKGVLRRAGHTEASIDLCRLAGL
                     RPAAALIEIMNEDGSMARLPELYKLAQKHKLKLIAIKDLIAYRLKQESLVEMGQSVQM
                     PTKYGFFKLIPFRQIATGQEHIALTKGEWSKDEAVLVRVHSSCLTGDVLGSMRCECGE
                     QLHKAMEIIEKEGKGVVVYMNQEGRGIGLMNKIQAYKLQEQGMDTVDANLHLGFDADE
                     RDYGVGAQILSKLGVRKMRLMTNNPIKRIGLEAYGLKIVENIPLEIPTNPYNEFYMKT
                     KKERMGHNLKNIK"
     gene            complement(11372..>12732)
                     /locus_tag="PLI37_04090"
     CDS             complement(11372..>12732)
                     /locus_tag="PLI37_04090"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOQ58230.1"
                     /translation="MVPLSLPLAVLLASLMTFGNFGEKLELLAMKSAGISLYNIMRPL
                     IVLMILLSVFSFVFQDRILPVAQMKLQSLTVSMQQKSPALSIPQGAFYSDIEGMTIYV
                     KEKDNKKKLLKDIMIYDFSQGFDNAAVTLADSGLLRLTEDKLNLVLTLYHGEGFSNFD
                     KQQAVTKDIPYRRESFREKEIIIPFDNNFNRIDESQFQSSHLSKKNDELKQVIDSMGS
                     LLDSTKIKKKIQYTESHFMGRNTEKGRELIGRDSMARATEVPLFNPDIDSLFLTLNKA
                     QADNMLDKALKKVGTIRSEFEYQEIALSREHNQFRRSGIEYYRRYALSVACLVFFFIG
                     APLGAIIRKGGIGMPAVVSVILFIAYYMIDAAGQKLVRNGTLEAFYGMFLSTAALLPL
                     GIFLTYKSANDSAIFNLDSYIAFFKRIFNVKFTYRYLRYVFRVALKKKYYARKNKTKQ
                     YR"
     assembly_gap    12733..12832
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12833..13071)
                     /locus_tag="PLI37_04095"
     CDS             complement(<12833..13071)
                     /locus_tag="PLI37_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOQ58231.1"
                     /translation="MFRIKRLHLFVLQSFFPIFLMTFLISNFILVMQLVWRVAEYIVG
                     KGIEFTAYLEFLLYAAATMVPLSLPLAVLLASLMT"
     gene            complement(13150..13524)
                     /locus_tag="PLI37_04100"
     CDS             complement(13150..13524)
                     /locus_tag="PLI37_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOQ58232.1"
                     /translation="MKEAFRLEYSLKGSVPVLWKLISTPNGLSEWFADNVQAEDSIYT
                     FQWGRTSEQAEVVSMVQGESIRYRWLDEPEDTYFEFNIQYSELTGNVSLWVTDYAEPE
                     DQEDAVNLWNYEIEKLMRRSGM"
     gene            13694..13767
                     /locus_tag="PLI37_04105"
     tRNA            13694..13767
                     /locus_tag="PLI37_04105"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13728..13730,aa:Met,seq:cat)
     gene            complement(13843..15084)
                     /locus_tag="PLI37_04110"
     CDS             complement(13843..15084)
                     /locus_tag="PLI37_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58233.1"
                     /translation="MKNLNMLALIALILAFCSCSQEQEVNTAALDVQTIGGYVQKGPF
                     LNGTSITFSELSEEFVPTGKNFSTQINDNKGSFELLNLTLVSPYVELEANGFYYNEVK
                     NENSAAQLTLYALSDLTDKSSLNVNVLTHLERNRVKHLIANGLSFSEAKSQSQREILS
                     LFEIDKQNVANSELLDITKQGDDNAILLAVSVILQGHLSISELSELLANISTDIREDG
                     LLNNPALGSMLINNAKYLNLENIRQHLENRYEALEMDVSIPDFEGYVNAFIENTDFVL
                     TRHIEYPAAGQHGLNILDREKTQYAAGDYSMKAVLPEGTNLKVKISGDNWVYPAMQNN
                     TGWDYSEWNATEKSRLFTSVKTGEIDFEIRFQYKENSGASQGDTINPPSGNTDLNKVN
                     LFVYENGTPEPTWTKEITITP"
BASE COUNT         3832 a         3505 c         3741 g         3929 t
ORIGIN      
        1 taaaatggta ccgataaata agcgaattgt ccacgagggt taaaaacacc cgacttttgc
       61 ggacttgccg gcagcgatga atagctctca ggtaaggttt tcagtaagta ggttttacca
      121 atcctccgcc tgcagctgtc tgccaaaaat ccgcagttgt cggcgggctt ccgggtagtt
      181 gggcatcatt cggccgagtt ttgtccagaa ttggtggctg tggttgtgtt ccagggtgtg
      241 ggccaattcg tgcagcacca cgtaatcgac gcagagttcg tctgccatga tgagtcgcca
      301 gctgaaaatg agccggtttt tggaactgca cgagccccag cgggttttgg ctgaacctat
      361 gcgtacgtcg gtgtatttga gcttgtacag acgggcgtaa tgctctactt tggctgtaat
      421 atgtgttgtg gcgagttctt tatagatttt cactaccgtg gcccggattt tttcttcggg
      481 aatcttgttg ccgaggtaaa aaccccgggc gtcgaatccg ctttttcgat tttcggaaag
      541 acgcagggga taagtcctgc ccaggtagcg gagttccatg cccgggtgca ggcggaaggc
      601 ttgccgtttt tcgaaatttt ccctgagcag ttgttgtttg gcgactatcc aatgctgttt
      661 ggcctgaata aagcgctcca cttcgcgcag gttcagtctc agagggcaac gcaccaagag
      721 gctgccgtcg ggcttgatct gcaaagttag ggttttgcga cgggaacgga tcagcgtata
      781 attcataata cagccttgaa cgacatatcg aggctacgta tggagtgggt gagggcgcca
      841 accgaaataa aatctacgcc gcattcggca taggcccgga tggtatcgag ggtgatgccg
      901 cccgaagatt cggtttcgta gcgtcccgct atgcgttcta cggccttgcg ggtggtggcg
      961 ggatcgaaat tgtcgagcat gatgcggtcc actcctccgc attgaagcac ctgctccagt
     1021 tcgtccaggg aacgaacttc gatttcgatt ttcaggttga gctttttttc ctgcagataa
     1081 tccctggtgg ctgcaatggc tttggctatg ccgcctgcaa aatcgacgtg attgtctttg
     1141 agcaaaatca tatcgtagag gcccatgcga tggttgctgc ctccgcccag taccacggct
     1201 tccttttcga gcaggcgcat gccgggagtg gttttgcgtg tgtcgatcac cttggtgtgt
     1261 aagccttgca gtcggtcggc atacaaacgg gtagtggtgg ctatgccgct cattcgttgc
     1321 ataatgttga gcatcagtcg ttcagtctgc aaaagagaac gaacgctgcc ggcggtttca
     1381 aaaacaatat cgccgggttt caccttttgg ccgtcctgta taaatacggt ggtttgcaat
     1441 tgcgggtcga agagcctgaa tacttccagg gctacccgta cgccggccac gactcccgct
     1501 tctttaatca gcaacatgga tttgccccgg gcggaagcag gaatgcagct caaactggtg
     1561 tggtcgcctt cgcctatgtc ttcggcaaaa gcattgcgga taaaatcttc taatgaggcc
     1621 ataatggttt atttcttcgg ggaattcatc gcaaaaatac ggtctttatt tcaaaaagcc
     1681 ctgcaagtgc ttcgctattt gtgcagaatt gccttttttt gcaggcatga aagtcatttt
     1741 attgttgtcg ggtaagaccg gcgaagatta cgcccgcaag gctattaaac tgtttgagga
     1801 aaggttgaag cattacttag ccttcgagat cgtttatttg ccggaactca aaagatcggg
     1861 caaaataagc gagcaggagc aaaaacaacg tgaagcggct ttgctggagc cctggattaa
     1921 accggagaat accctggttt tgctcgacga gagaggccgt gaatatactt cggaagcttt
     1981 tgccgcctac cttcaaaaaa agatgaacgc cggtttgcgc aacctggttt ttgtatgcgg
     2041 cggcccttat ggtttcgatg catctgttta taaacgggtt cccgagaggc tttcgctgtc
     2101 gaaaatgacc ttttcgcatc aaatggtacg ggtgtttttt gtggaacagc tctaccgggc
     2161 catgaccatt ctgaggggag aaccctatca tcattcataa aaccgctgcc gccaaaccac
     2221 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnattctgag gggagaaccc
     2341 tatcatcatt cataaaaccg ctgccgccaa accaccctcc gccgggttgc tacccccaat
     2401 ttgcttcggc aagccactgc cgccaaagcg gcaggctgtt ttcggcccgg tccgcctgct
     2461 tgttttatga gcgtttagat tccggcagaa agcgattccg cgcgaaaaca gccccaactc
     2521 ccattgaaaa acagaaaaaa taaaaaaaac gggcgtttgg ttggtacata taaaaattaa
     2581 gtctacattt gccccacaaa aacaaagaaa gatgtttgca cttgtagtat ctgtcctggt
     2641 gattatccta gtcgtggtgg cttcgggatc gggaaaggta tgttaataag tgtgcacaga
     2701 aatatagtat aggcctttcc catgaaacgg aaaggctttt tttttggtta attgctacaa
     2761 aaaatgaaac atccattacg cagcgccgta tgcacggaag gtcggagaat ggccggggcc
     2821 agggccctgt ggctggccaa cggaatgaaa aaggaacaat tgggcaaacc gatcatcgcc
     2881 atagtcaatt cttttactca atttgtaccc gggcatgtgc atctgcacaa catcgggcag
     2941 gaaatcaaag ccgaaattga gaaagccggc tgtttcgcgg cggaattcaa taccatagcc
     3001 atagacgacg gtatagccat ggggcacgac ggtatgttgt attccctgcc cagccgcgat
     3061 ttgattgccg acagcgtgga atacatggtc aatgcccaca aagccgatgc catgatttgc
     3121 atcagcaatt gcgataaaat taccccgggt atgcttatgg ccgccctgag gatgaacatt
     3181 ccctcggtgt ttgtttccgg cggccctatg gaagccggca agctgggcga tacttacctg
     3241 gatctgatcg atgctatggt gcaggcggcc gatcccaagg tatcggacga ggaactggcc
     3301 gccgtcgaag ctgtggcctg cccgggttgc ggcagttgtt cgggcatgtt tacggccaac
     3361 tccatgaatt gcctcaacga agccttgggc atggcgctgc ccggaaacgg caccgtgctg
     3421 gctacccatg tcaacaggaa aaaaatcttc caacaggccg cccggcagat agttcacaat
     3481 acgtttgcct attaccgcga cggcgatcaa cgtgtactgc cccgttccat tgcaagcaaa
     3541 gcagcattca tgaacgccat ggctctcgat atagctatgg gcggttccac caatacgata
     3601 ctgcatttat tggctttggc acaagaggcc gaggtcgatt ttacgatgga cgacatcgat
     3661 gcgctgtcgc gtaaaattcc ctgcctgtgc aaagtggctc ccagcagtca ttaccacgtt
     3721 caggacgtaa acagggcggg aggcatactc tctattatgg acgagttgga ccaagccgct
     3781 ttgatagaca attcggttta cagagtggac ggttataccc tgaaagaagc catggataag
     3841 tactcaatcc gttccgccaa cgtactgccg gaagccctgg ccttgtataa atcggctccc
     3901 ggcggtaaat tcaataatac cctcgcttcg cagggggccg tgtataaaga attggatacc
     3961 gatcgcagct taggctgtat ccgtgatatt gagcatgctt atacccgcga cggcggactg
     4021 gctatactca aaggcaatat cgcacaggac ggctgtgtgg tcaaaacagc cggagtggac
     4081 gaatccatct ggaaattcag cggcaaggcc aaagtctttc attcacagga agcggcctgc
     4141 gacggcatac tcgacggcag ggtagaagcc ggcgatgtgg tggtaattgt gttcgaaggg
     4201 cccaaaggag gtccgggtat gcaggaaatg ttgtatccca catcgtacat caaatcgaga
     4261 ggtttgggca aaagctgtgc cctgatcacc gacggccgtt tttccggagg aacatcgggt
     4321 ctgtctatcg ggcatatttc gcccgaagcc gcttcgggtg gcgctgtggc cctgattcgc
     4381 gacaacgaca tcattgacat agatattcca caccgtagca taaatctcag aatcggcgcc
     4441 gaggagttga atcaacgatg gctcgaggaa gaaaagaaag ccgccggagc cttcaaaccc
     4501 aagcgcgacc gcaaggtttc caaagcgctg aaagcctatg ccggtatggt cagttcggcc
     4561 gacaaaggag ctgttcgaat cattgaagag gattaaaaaa tggaaaagaa aaaaatcaca
     4621 ggggccgaag ccctcttgtt tgcctttaaa gaggaaaagg tagacactat tttcggatat
     4681 ccggggggag ccattattcc ggtgttcgat gctttatata cccacaagga aagactggcg
     4741 ttccatcaca tccttacccg ccatgaacaa ggggccgcac acgctgctca gggttatgcc
     4801 agggtatcgg gcaaggtcgg tgtggtcatt gttacttcgg gacctgccgc tgcgaatatc
     4861 gttaccggag tggccgacgc tatgctcgac agtactccct tggtggtcgt taccggacaa
     4921 acttcggtag ctgctttggg ttcggatgct tttcaggaaa ccgacgtgct ggggattacg
     4981 caagccataa ccaagtggag ttatcagatt cgcagggccg aagacatttc ctgggccgtt
     5041 gccagggcct tctatattgc ccgcgaagga cgtcccgggc cggtagtgct cgacctttcg
     5101 aaagacgccc aaaatcaact cattgaattc agctacgaga aatgccgtta catccgcagt
     5161 taccagccgg agcctgagct caatccggat aaaatagccc gggctgccca actcatcaat
     5221 caagcgaaaa aaccttttgc cgtcgtaggg cagggcgtcc tgctcggccg ggcggaacag
     5281 gaattgttca actttttgaa taaagccgat attccggctg cttcttcttt gctggggctc
     5341 gacgttatgc ccgtggatta caggctcaac atgggtatgg tgggtatgca cggcaacgtg
     5401 gctcccaaca aaaaaaccaa cgaatgcgat gtgcttatcg ccatagggat gcgtttttcc
     5461 gaccgggtta ccgggaaatt gagcgaatat gccccgcagg caaaagtcat tcattttgat
     5521 atagatccct ccgagatcaa taaaaacgtc caggtcgaag tgggcgtact gggtaatgtc
     5581 aaaaaaagcc tgcccgccgt tacggagctt ctcgataagg ccgatcacaa ggagtggata
     5641 gagagttttg aagaacacaa gctttgcgaa taccgcgaag tcgtgagcaa ggatttgtat
     5701 ccgaacaacg gtattctgac catggccgaa gtggtcaaat gtctttcgga caacaccggc
     5761 gaaaacacca tagtggtgac cgacgtggga cagaatcaga ttactgctgc ccgctatttc
     5821 agctcctata acacgcggaa aatagttacc tcaggcggtc tgggcaccat gggcttcggc
     5881 ctgccggccg gcgttggtgc ggcattgggc tcgcccgaat ccaaggtctt tgtgatcagc
     5941 ggcgacgggg gcattcaaat gaccatccag gaattgggta ccattatgga gtataaaata
     6001 ccggtcaaag tgatcatatt gaacaataat tacctgggta tggtgcgcca atggcaggaa
     6061 ttgttctacg aggagcgcta ttcgtccact tcgatacaaa atcccgattt tattgctttg
     6121 gctgccgctt acggcatcaa aggacgcaaa gtggaacagc gtaaggatct tgcggaggcc
     6181 gtcagagaaa tgattgccgc cgattcggct tatgtgctcg aagttatggt cgaaaagaaa
     6241 ggcatcgtct atccgatggt acctgccgga aaatgtatca cagaaatcat gttaaagagc
     6301 tgacattatg gaaaaaactt tatataccgt tacggtttat tccgagaatc aggtgggttt
     6361 gctcaatcag gtgaccattg tgttcaccag acggcaagtg aacatcgaaa gcctaacggt
     6421 ttcgcgttcg gccataccgg gtgtacataa attcactatt accgctaaca ctgaccggga
     6481 aaccatacgc aagattgttt tgcagatcga aaaacgcatt gatgtgctca aggcctatta
     6541 ttataccgac gacgagatca tatttcaaga aattgcactc tacaaagtgc ctactttaag
     6601 tttgatgtcg agcaacggaa tcgaaactct ggtacggaaa tacaatgcac gtattctgga
     6661 agtaaccaaa gactataccg ttattgagca ggcgggacat aaagccgaaa tcgacgctat
     6721 gtttgacgct ttaaacaaaa acaaaattct gcaatttgtt cgttccggac gggttgccat
     6781 taccagatcg acggtggaac ggctgagtaa ttttctggaa gagatggaag ctcgcagaaa
     6841 agaagtattt aatggaaaca actaatgtct tatcaataaa tttatattta tggctaaaat
     6901 gaatttcggt ggtgtaatgg aaaatgtcat caccagggaa gaattcccgt tgaaaaaagc
     6961 aaaagaaaca ttgaaaaacg aaaccatagc cgtgataggt tatggtgtac aaggccccgg
     7021 acaatccaag aaccttcggg acaatggttt caacgtaatt gtgggacaac gcaaaggcgg
     7081 tgccacctgg gataaggctg tggccgacgg ttgggtgccc ggcgaaaccc tgttcgaaat
     7141 cgaagaggcc tgccgcaggg gaaccatcat acagtatttg ctttccgatg ctgcccagat
     7201 agaagtctgg cccagaatca aacctcacct gacagcaggc aaagccctgt atttttcgca
     7261 tggttttgcc atcacctata aggatcgtac caacattatt cctcccaagg acatcgatgt
     7321 tattctggtg gcacccaaag gttcgggtac ctcgctgcgc accatgtttc tcgaaggtcg
     7381 cggcttgaat tcgtcctacg ctatttttca ggatgccacg ggtaaagcct tcgaccgggt
     7441 ggtggccctg ggcatcggag tcggatcggg ctatttgttc gaaaccacct ttcagcgcga
     7501 agtgtattcc gatcttacgg gcgaacgcgg cactctgatg ggcgccattc agggcctgtt
     7561 gctggcacaa tacgaagtgc tcagagccaa cggacatacg ccctcggaag ccttcaacga
     7621 aaccgtcgaa gaattgacac aatccctgat gcccttgttt gctcaaaaag gaatggactg
     7681 gatgtatgcc aattgctcca ccactgctca acgcggcgcc ctcgattgga tgggaccttt
     7741 ccacgatgca gtgaagccgg tcttcgaaaa attatataaa gaagtggcca acggcaacga
     7801 agctcaacgc tccatcgact ccaactccaa acccgattac agggaaaaac tcgaagaaga
     7861 actccgccaa ttgcgcgaaa gcgaaatgtg gcagaccggt gccgtggtac gcaaattgcg
     7921 ccccgaaaac aattaggcca atttagccaa agccacttta atacgatcga tcgctttaac
     7981 aatgttttcg tcggaggtgg cgtaggagaa acgcaaacaa ttgggtgcgc cgaatgctac
     8041 gccgcctacc gccgctacat gagcttcttc gagcagaaac atagccaggt cggtggcatt
     8101 ggttatggtt ttgtccttga acgatttgcc tatataagcg ctgcaatcgg gaaaaacata
     8161 gaaggctccc ttgggttcct gtaccttgaa accgggaact tcgcgcatca attttacgat
     8221 aaggttttta cggcgttcga aggctgtgcg catttcggcc acacaagcct ggtctccggt
     8281 gtaagcagcc cgggcggctt cctgtgaaac cgagcagggg ccggaggtat attgtccctg
     8341 caatttgttg caggcggaag ctatccactg tggagcggca atccagccga tacgccagcc
     8401 ggtcatggca taagctttgg acacgccgtt gatgacgatt acccgttcgc gaacggactc
     8461 aaactgggca atgctctcgt gtttgcccaa gtaattgatg tgttcgtaaa tctcgtccga
     8521 aagtatgtaa acctgaggat gtttggccag gacagcggcc agagcttgca attcttcttt
     8581 gctgtaaacg ctgccggtag ggttggaggg agaacagaga atcaaggctt tggttttggg
     8641 cgtaacggcc gcttcgagtt gggcggctgt tattttgaaa tcttgctcta tgccggcaga
     8701 gactattaca ttctttcctt ctgccaattt gaccatttca acatagctta cccaataagg
     8761 agcgggaacg atgacttcgt cgcccgggct gataatggaa agcagcacgt tgcataccga
     8821 ttgcttggct ccgttggaac aaacaatctg atcgacggta taatcgaggc cgttctcttt
     8881 tttcagtttc tccacgatgg ccttgcgcag agccgggtaa ccgggaacgg gagaataaaa
     8941 ggaaaagtta ttgtcgacgg cttgtttggc tgccagtttg atgtggtcag gggtaaaaaa
     9001 atcgggttcg ccgacactca tgttaataac atcaaaaccc atagctttga gttcgttgct
     9061 cttttgagac atggcaaggg tttcggaagg ggccaggctg gccaatcgga gggatacggg
     9121 gttcattcta gtatggttaa taattttcgg tgcaaagcta aactaattcc attttaattc
     9181 tgcggatttt catccatttt cagcaaatta tttaaaactt cttgttcaac ctcgaaagcc
     9241 gtaccgtggc gtacacccga gggaaagctg atgatttttg aaatatcgtc ccagacgcct
     9301 tttttcaggt ctttgcatct ggctgccccg tagcgttggt tgcggtattt atcccaataa
     9361 acataagtgt atttacctat ttgcagaggg gtggggcctt cggcccattc gatgccgctg
     9421 ataggttcgg ataccgtttt ggggaaagcc gtcagttttt tggaagctac cacacgtatg
     9481 tttttttcga ccggttccag ggtttcgttt ttcaggaaca tataatagat tcctttgtgt
     9541 ttgaggatgg cggcatcgat aacattgaat tccggatcgt aaaacaattc ggtgggacta
     9601 aaacgttcaa agtcttttgt tgtagtataa tacaaacgat gattgtaatc gctttcgctg
     9661 ctgcccggtg ccggaaaacg ccccggaatg gtactggccc aaatgatgta atactgtttc
     9721 gattttttgt cgtaaaacaa ttcgggagcc caggtgtttc tgacttcggg ctcgtgggcc
     9781 attacgggaa tttccctttg ttccgaccaa tggatcaggt cggtggaaga agcgtaaccg
     9841 atgttgtttt cggtccagcc tgtagtccac accatatgaa ataccccgtc gggagcctga
     9901 actatactgg gatcccgcat caatttggcg gtgccgactt ccggtttcag aaaaatctcc
     9961 ccacggttca ggttcttcca taaaatgccg tcgtagctgt acaaaaaatg cagaccgtcg
    10021 ccgccgccca tgaaatagct gaacaaataa gcctttttgg gccgttcgat caaatcccaa
    10081 aaactttttt tatcggtttt ctctttgctt ttggcctgga catcccatgt gccgcacagg
    10141 aatactgtca ggagaataag tagagcagtt ctttttttca tactcggttc atttaatgtt
    10201 tttcaaatta tgccccattc gttctttttt ggttttcata taaaattcgt tgtagggatt
    10261 ggtgggtatt tcgaggggaa tattttccac gattttcagt ccgtaggctt ccaggcctat
    10321 gcgttttatc ggattgttgg tcatcaggcg cattttgcgt acgcccaact tcgaaagtat
    10381 ctgagctcca acgccataat ctctttcgtc ggcgtcaaag cccaggtgca aattggcatc
    10441 gaccgtgtcc ataccctgtt cctgcaattt ataggcttga attttattca tcagcccgat
    10501 gccgcggcct tcctgattca tgtaaacgac aacgcctttg ccttcttttt cgatgatctc
    10561 catcgctttg tgcaattgtt cgccgcattc gcagcgcata gaacccagta cgtctcccgt
    10621 gaggcaggaa gagtgtaccc tgaccaatac agcttcgtct ttgctccatt ctcccttggt
    10681 cagagcaata tgttcctgac cggtagcaat ttgcctgaag ggaatcaatt tgaaaaatcc
    10741 atatttggtc ggcatctgaa cgctttgacc catctcaacc aaagattcct gtttgagccg
    10801 gtaagcaatc aggtctttga tggctatgag tttcagcttg tgtttttgtg ccagtttgta
    10861 caattcgggc agacgggcca tgctgccgtc ttcgttcatt atttctatca gggcggctgc
    10921 cggccttaaa ccggccagac ggcacaggtc tatggaagct tcggtgtgtc ccgcccggcg
    10981 caatactcct ttgtttttgg cgtaaagggg actgatgtgt cccgggcgtc ccaggtcttc
    11041 gggtttggtg ttgggatcgg ccaaagcctt gatcgtttcg gcgcgatcgt gcgaagatac
    11101 cccggtggta caaccttcca gtttatcaac ggtcaccgta aagggggttc ccaaaatgga
    11161 ggtgttattc gccacctgtt tatccaactc caattccaaa caacgttctt cggtgatggg
    11221 tgcacagagt acgccgcgtc ccacggtaat cataaagttt actttctcgg gcgtaatcaa
    11281 ttcggccgag gtaataaagt cgccctcgtt ttcgcggtct tcgtcgtcga ccacgatgat
    11341 gatttctccc tggcgtatgg cttctatagc ctcatctata ctgtttagtt ttattttttc
    11401 ttgcataata cttctttttt aaagcaactc taaaaacata gcgcaaatag cggtaagtga
    11461 attttacatt gaatatcctt ttgaaaaaag cgatataaga atccaaattg aaaatggcgg
    11521 agtcgttggc cgatttatag gtaaggaaaa tgcccagagg caacaaggca gcggtgctca
    11581 ggaacatacc gtaaaaagct tcaagggtac cgttgcggac caatttttgg ccggctgcat
    11641 ctatcatgta ataagcgata aacaaaatca ccgaaaccac ggcaggcatg cctatgccgc
    11701 ccttgcgaat aatggctccc aggggtgcgc cgataaaaaa gaaaaccagg caggcaaccg
    11761 agagggcata gcggcggtaa tattcgatac cgctgcgtct aaactgatta tgttcccttg
    11821 ataaggcaat ttcctggtat tcaaactcgg atctgattgt gccgactttt ttcagagcct
    11881 tgtccaacat attgtctgct tgagctttat taagagtcag gaacaaactg tctatgtcgg
    11941 gattgaaaag cggaacttcc gtagcgcgag ccatggaatc tctgccgatg agttcacgac
    12001 ctttttcggt attcctaccc ataaaatgac tttcggtgta ctgtattttc tttttgattt
    12061 ttgtgctgtc cagcagactg cccatagaat cgattacctg cttgagctcg tcgttttttt
    12121 tgctcagatg cgaagattga aactgagact catcgatacg gttgaagtta ttatcgaagg
    12181 gaatgatgat ttctttttcc ctgaagcttt cgcggcgata gggtatatct ttagtgacgg
    12241 cctgttgttt atcaaaattg ctgaagccct cgccatggta taaggtcaaa accaggttta
    12301 atttatcctc ggtgagtcgc aacaatccgg aatcggccag agttactgcc gcattgtcga
    12361 aaccctggct gaaatcgtaa atcatgatgt ctttgagcaa tttctttttg ttgtcttttt
    12421 ctttgacgta aatggtcatg ccctcgatat cggagtaaaa agctccctga ggaatggata
    12481 aggccggcga tttttgttgc atggaaaccg tcagcgactg cagtttcatt tgagccaccg
    12541 gtaaaatcct gtcctgaaaa acaaaagaaa agaccgacaa caggatcatc agcactatca
    12601 ggggccgcat aatgttgtaa agcgaaatac cggccgattt catggccagg agctccagct
    12661 tttcaccgaa attgccgaag gtcatcaggg aggccagcaa aacagccaaa ggaagcgaca
    12721 ggggaaccat ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaggtcat
    12841 cagggaggcc agcaaaacag ccaaaggaag cgacagggga accatggtgg cagcagcata
    12901 gagcaaaaat tccagatagg cggtaaattc tatgcctttg ccgacaatgt attctgccac
    12961 tctccacacc aattgcatca ccagaataaa attgctgatg aggaaagtca ttaaaaagat
    13021 ggggaaaaaa ctttgcagaa caaagagatg taaccttttt attctaaaca taaatcgact
    13081 ttgtttaagc ctgcgaaggt aatctaaaaa aacgagccgg gcacagcaaa gcaggaatta
    13141 tgcacgagat tacatgccgg agcgcctcat caatttttca atctcgtaat tccaaagatt
    13201 gacggcgtcc tcctgatctt cgggttcagc ataatcggta acccacaaag agacgttgcc
    13261 ggtcagttcc gaatattgta tgttgaattc gaaataagtg tcttccggtt cgtcaagcca
    13321 acggtagcga atggattctc cctgtaccat agaaaccact tcggcctgct cgctagttct
    13381 accccattga aaagtataaa tcgaatcttc tgcttgtaca ttgtctgcaa accattccga
    13441 cagaccgttg ggtgtgctta tcagtttcca cagcaccggg acagatccct taagagagta
    13501 ttccaacctg aaagcttctt tcatcacgag cgttttttga attttgccga aagtacatat
    13561 tttttctgaa taatgagcca caatagaaag ataaattccg gatagttttc aaaaagttgg
    13621 ggattttttt atttccctgt ttggttggat aaactaaaaa gattaccttt gccgcgcttt
    13681 tgggcaagca gatggcgaga tagctcagcc ggttagagcg catgattcat aatcatgagg
    13741 tccccggttc aatcccgggt ctcgctacaa tcaaaaaaga gggcaaattg ccctcttttt
    13801 gtattggatt accgaacaat ccaaattatc gacctgcccg atttagggcg ttatcgttat
    13861 ttcttttgtc caggtaggtt ccggggtgcc gttctcgtaa acgaataagt ttaccttatt
    13921 caaatcggta tttcccgaag gaggattgat cgtatcaccc tgagaagctc cggaattttc
    13981 tttgtattga aatcgtatct cgaaatcaat ctctccggtt tttacggaag taaacaggcg
    14041 tgatttttcc gttgcgttcc attccgaata atcccagccg gtattattct gcattgcagg
    14101 gtacacccag ttatcgccac tgatcttcac cttgagattg gttccttcgg gcaatacggc
    14161 tttcatggaa tagtctccgg ccgcgtattg ggttttttct ctatcaagta tgttcaaacc
    14221 atgctggcct gctgccggat actcaatatg acgggtgagc acaaagtcgg tattttcgat
    14281 aaaagcattt acataccctt caaaatcggg tatggatacg tccatctcta aagcttcgta
    14341 ccggttttcc agatgttgac gaatattttc caagttgagg tatttggcat tattgatcag
    14401 cattgagccc aaagccgggt tgttcaaaag accgtcttcc cgtatgtccg tgctgatatt
    14461 cgccaatagt tcggacaatt cggaaataga cagatgtccc tgaagaatca cggaaacagc
    14521 caacaagata gcgttgtcgt ctccctgttt cgtaatgtcg agcagttccg aattggcaac
    14581 attctgttta tcaatctcga aaagggacaa aatttctctc tgcgattgac tttttgcttc
    14641 agaaaaactc aggccgttgg caataaggtg tttaactcta ttcctttcca ggtgagtgag
    14701 cacattgaca ttcagactgc ttttatccgt caaatcggat agagcataca aggttagttg
    14761 agcggcggaa ttttcatttt taacctcgtt gtagtagaag ccgtttgctt ctaattcgac
    14821 gtaaggagag accaatgtca gattcaacaa ctcaaagctt cctttgttat cgttgatttg
    14881 ggttgaaaaa ttctttcccg tcgggacaaa ctcttcagaa agttccgaaa aagtaatgga
    14941 cgttccattc agaaacggtc ctttttgaac gtagccccca atcgtctgca catccaatgc
    15001 tgccgtattt acctcttgtt cctgtgagca ggagcaaaac gcaaggataa gggctataag
    15061 cgcaagcatg tttaagtttt tcataatgtt tatattttat attgaattaa ataattaagg
    15121 caatgagata taagcatcgt gctatttctc atggcaacaa cactttaccg gtatagcatt
    15181 taccggcata aattttatac aagtaca
//
LOCUS       DAOSTD010000039        14517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_4237_length_14517_cov_14.043424, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000039 DAOSTD010000000
VERSION     DAOSTD010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14517
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4237_length_14517_cov_14.043424"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="PLI37_04115"
     CDS             complement(<1..1196)
                     /locus_tag="PLI37_04115"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HOQ58234.1"
                     /translation="MKKASFLIAFIALLLSSCCCKENALTVQVNNPSALDRERESIEI
                     ELDAINNALGIKNDTTSRFVVFDAQGQEIPSQLILSSTPATLLFQATVKAGDSCTFTI
                     KTGQPTPYESMVQTTFMPRRLDDMSWENDKVSFRAYGPALAKDPQQGMVSGGLDIWVK
                     RTSKLVTQQWYQDEFDGKSSYHNDNGEGLDFYSVGKTLGAGSLAPFIEGKLWLVGNNF
                     VSYKILENGPIRTTFVLEYEAYDCNSKALSETRTISLDAGSQLNKFSILYKGNDGEVI
                     PVAAGFPFRGFEQKSGRKESHGIDDCNSIAYDKTLGYIAYAEPAHAENGIIFLGSLMP
                     EGSDQALIAQGHVLNGSHATIGKEFVYYSGGGWSKGGFSDLVAWTAYMKDFALKLREP
                     LKVSLEA"
     gene            1354..2742
                     /locus_tag="PLI37_04120"
     CDS             1354..2742
                     /locus_tag="PLI37_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOQ58235.1"
                     /translation="MKTKIFILCLLLGTSLTLQAKKTIKVACVGNSITQGAAISNQQR
                     DSYPGLLGQMLGAGYEVRNYGFSGRTLLNKGDRPYMKEQMFLDALAFEPDIVTIKLGT
                     NDTKPFNWVHHHEFMRDLEQMVNAFSRLSSKPQIWLCYPVPAFHYDWGINDTIVRNQV
                     IPYIRKVAQKHDLNIIDLYTPFIGKGHLFADGIHPNEEGALVLAKEVYRALKGEEVPV
                     GYRPQAYPGVRSDWQGYERYNFNFREKQATLVVPKQAAEGKPWIWRPAFFGAFPSVDL
                     ALLEKGYHVVYFELDNEFANPASLKAGDDFYKYMVKYYALSPRVVLEGFSRGGLYAFN
                     WALSYPQKVACLYLDAPVCDVYSWPGRERPEWQDFLKKWRLGEDQLEQFTANPVDNTE
                     ALVATQIPLILVAGTKDKLVPYKHNGAHLYQAYKKAGLEVKQILKKGGGHHPHSLEDP
                     AEIVEFILEHTK"
     assembly_gap    2768..2867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3130..4572
                     /locus_tag="PLI37_04125"
     CDS             3130..4572
                     /locus_tag="PLI37_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOQ58236.1"
                     /translation="MKTPNIISLVLIALLITACGPSQLQNGIPNFPWIAECGSQTQPP
                     VDKKFMANDYGALADTTQLSTEAVQRAIDACAEAGGGMVSFEPGSYCIGSIFLRSNVN
                     LNIPEGVVLLGSENILDYPLISTRVAGIEMEWPAALITAIDQDNVAVSGRGLVNGRGK
                     VFWDNYWEMRREYVPKGLRWIVDYDCKRPRLLLMQNCSNVTLSEAKFIEAGFWTVHIL
                     YSTQVTIKNLTIDNNNAGRGPSTDGIDIDSSNRILIEDCDISCNDDNFCLKAGRDADG
                     LRVNRPTEYVVIRNCIARYGDGLFTCGSETSGGIRNVVAYNLEAIGTKIGLRFKSAYT
                     RGGVVEDIYLANIKMRGLREPLQINLNWNPSYSYSSLPEAYDINEVPPHWKVMLTPVD
                     PPELGMPKFRNVHFTDIKADSCSVAITCEGAENSTIDQFYMNNVHITGAKAGRIYWAK
                     DWKLKNVWIGSEKGEMPDIQNTVNVPLLQE"
     gene            4617..6770
                     /locus_tag="PLI37_04130"
     CDS             4617..6770
                     /locus_tag="PLI37_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58237.1"
                     /translation="MKNLLLTATLALGIFVSCQQAGQESIDRKALVDRNKVQVNAFDS
                     LASLSLGNGNFAFTVDITGLQTFPERYSKGVPLGTQSQWGWHSFPNTQKYRFEETLRA
                     YNFRGKEELYAVQFNEKGRPQAAADYFRINPHRLHLGVVGLELRNPQGEIMRPEDLSE
                     INQELDLWNGTINSLFKAGEFSVHTQSYCHPEMDMMASSIESSMFLSGNLRLRLAFPY
                     PSGNHSDDACDWSSPGKHSTKVLAQKPGAVLLERRLDNDSYYVLLCWKEQAEFISGPA
                     AHHFYLQPAGEKLSFTCLFLERNPLLDSSELPACPESEQVAQASSDYWNQFWLSGGAV
                     DFSACTDPRAPELERRVVLSQYLMAIQCAGVYPPQESGLTYNTWYGKFHLEMHWWHGV
                     HFVLWGRPELLERSLDWYRTAEANAANIAQRQGFKGVRWMKMTDPSAIEAPSKVGSFL
                     IWQQPHIIYMAELLFRHQKDSSVLADYSDLVFKTAEFMADFADYDGMGDRYILQGAIP
                     AQETLRASETVNSPFELSYWHYALSVAQQWRLRLGLPKNMEWEIVLDKLSPLAHKDGL
                     YLAAETALDTYIDTRYTSDHMAVLGALGMMPKSRLVREDIMTNTFNWIWDNWNWGKTW
                     GWDYPMTAMCAARIGLPEKAVDALLMDRRTNTYLVNGHNYQDGRLRVYLPGNGGLLTA
                     VAMMCAGWDGSEGKNPGFPNNGQWKVKWEGLDVMP"
     gene            6786..8129
                     /locus_tag="PLI37_04135"
     CDS             6786..8129
                     /locus_tag="PLI37_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOQ58238.1"
                     /translation="MKWILRVFPALFLIFSAGLTEVNAQNKLAFPGAEGFGRFATGGR
                     GGSIYHVTNLNDSGEGSLRDAVSRPNRIVVFDVSGVIRLQSALVFSKNLTVLGQTAPG
                     GGIQVYGDQVSFSGADNIIVRYLRIRMGIDGTDGKDAAGVANGKNMIFDHMSVLWGKD
                     ENFSVSWGNRGTEPTNITIQNSIIGQGLQPHSCGGLIQTRGGVTLFRNLFIENNTRNP
                     KVKGLNQYVNNVVYNWGDGCYIMGGSEGPSWAHIENNYFINGPWEGATPVFTRGNVNF
                     TFYGAGNYHDANRNGLLDGSLMTADQYSASIPVSDWATWDNSTARPQPHPEILSMTTA
                     QEALAWIIDSVGPCLPVRDEVDQYLIDELVSYGTKGTANGIISEKTLPHKGTGTLRGG
                     YRPPDTDQDGIPDAWETANGLNPNDPSDAAAIAANGYSNIENYSFSITACIKSPL"
     gene            8203..9624
                     /gene="cls"
                     /locus_tag="PLI37_04140"
     CDS             8203..9624
                     /gene="cls"
                     /locus_tag="PLI37_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOQ58239.1"
                     /translation="MNVVFQQIVYALVALTVLSVVLVIISENRNPVKSVAWILLLIFL
                     PLLGLIAYFLIGQDFTKKYAYKRKKHRGKDQAFLIKSDQVLDEQEYPRAFTAPVKLLK
                     NIQNALLIGGNDVKYFTLAADKYRQMLADMEEAKRFIHLEYYALENGLWGDALKDLLI
                     RKSARGVEVRIIYDDWGCWRTKKSYFKELIDNGVQVRVFSKVRILPLTARLNYRDHRK
                     LIIIDGLIGYIGGMNISDRYVNGFNWGCWADTHCRIAGPGVVQLQSVFLSNWQRQSGE
                     VLWEDEYFPLPASCGDNALQVVASGPGSLDPEILHVMIQAFYSAEKYIYVQTPYFIPP
                     EPLAGALQAAAIRGLDVRLMISKRSDLPLVQMASRSYIREMLERGIKVYFYTRGFLHS
                     KMMVVDGELSIMGSANADMRSFEDNLEVQAFIYGRSAALRAQEIFLEYQQDCEKINLE
                     RWKKRSRLKRFVESFMRLFAPLL"
     gene            complement(9638..14053)
                     /locus_tag="PLI37_04145"
     CDS             complement(9638..14053)
                     /locus_tag="PLI37_04145"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOQ58240.1"
                     /translation="MKKFLLFLCLSVVAVMFSYGQRLMENLDRGLTAVKVSNGVFVSW
                     RIPGSEWNNTQYNLYRNGVKLNTEPLSVSNYLDEGGSNASTYTLGLVKEGVESPEPGN
                     YAVWNKQYLEIPMRQIIKNGIDYGPLYELNDATAADLDGDGQYEIIVKRINSDFSVAN
                     DSAFSYFEAYKLDGTLLWAIDIGPNLLSSGHVETNIAAFDWDEDGKAEVIMRAADGTI
                     DGKGNVIGSPTANYRSYVNQSPNMQFMIEGPEFLCLFDGETGELLDKVDYIARGNVND
                     WGDGYGHRANKFFFGAPYLDGRRPSILTTRGIYTRIVMRAYDVVDKKLQKRWLSDFDT
                     NNSLWSAYAYQGNHNYTIADVDGDGRDEIVYGSMTVDDNGRGLYSTGYGHGDAMHVSD
                     FDPYHRGLEIFACLESSPHWGAAFRDGATTETFIKYVRGRDCGRCMAANVTDDYPGAE
                     LWADGKMWSATTRELVGSSGGTQNFAIYWDGDLLRETFDYTNVNDVTGGYGYGTPAVF
                     KYNKGSVSNIFTATGTATNNYTKGNPCLQADLFGDWREEMVLRSTDNKYLRIYTSVVP
                     SPWPIYTLMHDHQYRQAICWQMCGYNQPPHTSFFLGEREGITAPPPPWISNGKTEVDG
                     FISGDHQGKELMLANTQGAEVELRSKVSPASVNVNSPGDYRLYGTGELQGPMTLHKQG
                     LGNLTIEGAQTYSGNTEVWDGLLTLKGSLAGPLLLRRFAELDAYNSLEGGLQMEYGAI
                     LRPGGSGKQSGLRLKSIKMDKGAVVEFNLSDADFTCDTLIIQENLELSDGAVFRFVKP
                     RPDFKPQPGDYLFAVCEGIVTAELNKIVIEGLPGVACSVLNQNDSLFLHVDEQRAAQL
                     VYWHGKFDQGIWNLNNTRNFYIGQDTVEFVTNDTVVFDQNTGARAVNITEEVIPSEVR
                     IQGDLNYAITGNGGIGGSSSLIKEGQGKLSLLNTNPYTGKTLIKGGTVEVSSLATSQG
                     AGSLGALSSVSNNLTIQNGAILKTTGNISMESPLYLGPEGGCLDITNTLTMKGVISGG
                     TLIKRGNGTLEVTAVNTHKKTILEGGTLKVLEEPNLINGYFGDTLVLNGGVVICLDGN
                     SSYSRANWNIVVPAGKSATIYLDGRCEYHGSLHGSGTLTLSAPYVRNYLCGNWSAFEG
                     VVKATSKANSSYTPSFDFKNSYGLPKATLDVAAGTTVTNQSGNFKLGAVSGNGTLGGS
                     NAWEIGYLNTTNSFSGTIASALTKVGTGTLLLSGANNYTGDTNINGGILRVNNANATV
                     SATGTGRLNINNGGTLTGRGFIDNNIVYINSGGLFIPGAYYVGELKVDGSIYQNPGGV
                     LEFRLNSATSHSSIKGLNVMTLKGTLRILLKEGYTPSLGDSFELWSCTSFSSSIPELV
                     LPELPANLAWDTSELFTGRGKLSVTDANGLQLNSWDEMLDIQVVNINGLTVASLESKA
                     TEWEKDVKELNLAKGIYLIKLQSGKSSRIVKHYQP"
     gene            complement(14080..>14517)
                     /locus_tag="PLI37_04150"
     CDS             complement(14080..>14517)
                     /locus_tag="PLI37_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOQ58241.1"
                     /translation="ALARYMEIAPADEKHRDQYLKDFKAMSKALLACQREDGYWNISL
                     HDPGHFGGKETSGTALFVFGMTWGIRHGILPAKIYLPAVLKGWKALEEAVHPNGFLGY
                     VQGTGKEPKDGQPVGYDIEPDFDDYGLGCFLLAGSEIYKLDLE"
BASE COUNT         3265 a         3627 c         3835 g         3690 t
ORIGIN      
        1 gcctccaagc tgaccttcaa aggttctctg agcttcaaag cgaagtcttt catataggct
       61 gtccaggcga ctaaatcgct gaaaccgcct ttgctccagc cgcctccgga atagtatacg
      121 aattctttgc cgatggtggc gtggctgccg ttgagtacgt ggccctgggc gatcagtgct
      181 tggtcggagc cttcgggcat gaggctgccc aaaaagatga tgccgttttc ggcgtgtgcg
      241 ggttcggcgt aggctatata acccagggtt ttgtcgtagg cgatgctgtt gcagtcgtcg
      301 atgccgtgcg attcttttct gccggatttc tgttcaaaac ctctgaatgg gaagccggca
      361 gccaccggaa tcacttcgcc gtcgttgcct ttgtagagta tgctgaattt attcagttgc
      421 gagccggcat cgaggctgat ggtgcgggtt tcgctcaggg ctttgctgtt gcagtcgtag
      481 gcttcgtatt ccagaacaaa cgtggtgcga atggggccgt tctccaatat tttgtaggat
      541 acaaagttgt tgcctaccag ccagagtttg ccctcgataa agggtgcgag gcttccggct
      601 cccagggttt tgcccacgga gtagaagtcg agcccttcgc cgttgtcgtt gtgataggag
      661 gatttaccgt cgaattcgtc ttgataccat tgttgggtga ccagcttcga tgtgcgtttt
      721 acccagatgt cgaggccacc gcttaccata ccttgttggg gatcttttgc cagagccggc
      781 ccataggctc ggaagctgac tttgtcgttt tcccaggaca tatcgtcgag gcgacgaggc
      841 ataaaggtgg tctgcaccat gctttcgtag ggcgtgggct gtcctgtttt aatggtaaag
      901 gtgcaggagt cgccggcttt gaccgtggcc tgaaacagaa gggtggccgg tgtggaactg
      961 aggataagct ggctggggat ttcctgacct tgagcgtcga agacaacaaa acggctggtg
     1021 gtatcgtttt tgattcccag cgcattattg atggcgtcca actcgatttc tatgctctca
     1081 cgctctctgt ccagagcgga agggttattt acttgtacgg tcaaagcgtt ttctttgcaa
     1141 caacaagagc tgagcaagag tgctatgaag gcgataagaa aagatgcttt tttcatgata
     1201 tgaaatgtta aaaatgtgat aaatcgctaa gttcctctaa tgtgaagcac aaatgtaggt
     1261 acaaggagtt aaatagacaa agttttgtta gcgtattaac agttaaattg tatttttgct
     1321 ctccgaaaac gccgtttaat gtaagatata ctaatgaaaa ctaaaatttt tattttgtgc
     1381 ttgttgttgg ggacaagtct gaccctgcag gcaaagaaaa ccatcaaagt ggcctgtgtg
     1441 ggcaacagca tcacccaagg ggcagccatt tccaatcagc aacgcgacag ctatccgggt
     1501 ttgctggggc agatgctcgg agccggttac gaagtgcgca attacggatt cagcggccgc
     1561 accctgctca ataagggcga ccgcccctat atgaaggagc agatgtttct cgatgccctg
     1621 gcctttgaac ccgacattgt taccatcaaa ctgggtacca acgataccaa acccttcaac
     1681 tgggtgcatc accacgagtt tatgcgcgac ctggaacaaa tggtgaatgc ctttagccgg
     1741 ttgagctcaa aaccgcaaat ctggttgtgc tatcctgtgc cggccttcca ctacgactgg
     1801 ggcatcaacg atacgatagt ccgcaaccag gtgatccctt atattcgcaa ggtggcccaa
     1861 aaacacgact tgaacatcat cgacctgtac acgcctttta tcggtaaagg acatttgttt
     1921 gccgacggca tccatcccaa cgaagaggga gctttggtgt tggccaaaga agtttaccgt
     1981 gccctcaaag gtgaggaagt gccggtcggc tatcgtccgc aggcgtatcc cggtgttcgc
     2041 tccgactggc agggttacga acgctataat ttcaatttca gggaaaaaca ggctaccctg
     2101 gtggtgccca aacaagccgc cgaaggtaag ccctggattt ggcggccggc ttttttcgga
     2161 gcttttcctt cggtcgacct ggccttgttg gaaaaaggct atcacgtggt ttattttgag
     2221 ctcgacaatg aatttgccaa cccggcttcg ctcaaggcag gcgatgactt ctacaaatac
     2281 atggtcaaat attacgcctt gtcgccccgg gtcgtgctcg aaggcttcag ccgcggaggc
     2341 ttgtatgcct ttaactgggc tttgagctat cctcaaaaag tggcttgcct ttacctggat
     2401 gcccctgtct gtgatgttta cagttggccc ggccgggagc gtcccgaatg gcaggatttt
     2461 ctgaaaaaat ggcgactggg cgaggatcaa ctcgagcaat ttacagccaa cccggtggac
     2521 aacacagaag ccctcgttgc aacgcagatt cctttgatac tggtggccgg caccaaagat
     2581 aaattagtgc cttacaagca caacggagct catttgtatc aggcctataa aaaggccggc
     2641 ctcgaagtga agcaaatcct gaaaaaaggc ggagggcatc atccccacag cctggaagat
     2701 ccggcggaga tagtggagtt tattttggag cacactaaat aaaaagccgg tctcttattg
     2761 gggcttcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctt cccgaggggc
     2881 ttttcttgcc cgggagttct cctgcatggg tctttttcct gccggaaagc ttctcttccg
     2941 atagacttct cctggcggtc ctattcctgt cggaaagttt ctctgtccgg ggccttttct
     3001 tgcgggggcc tttctcgccc gggagctttt tcttgccgga gcctttctac caatttttta
     3061 gctcgcttgc ttttttgttc tttagcggat aaataaatat ctttgttgct gccgttaatt
     3121 ttagtagtta tgaaaacacc caacatcatc agtctggtcc tgatagccct gttaatcacg
     3181 gcttgcggac cttctcaatt gcaaaatggt atccccaatt ttccctggat agccgaatgt
     3241 ggttcacaaa cccaacctcc tgtagataaa aagtttatgg ccaacgatta cggagcgctt
     3301 gccgatacaa cgcagctcag taccgaagcc gttcagcggg ccatcgatgc ctgtgccgaa
     3361 gccggcgggg gcatggttag tttcgagccc ggcagctatt gcataggttc catttttctc
     3421 agaagcaatg tcaatctgaa tattcccgaa ggggttgttc tcctgggcag cgaaaacatc
     3481 ctcgattatc ctctgatctc tacccgggtg gccggtatcg aaatggaatg gccggcagca
     3541 ctgatcaccg ccatcgatca ggacaacgtg gccgtttcgg gccggggact ggtaaatggc
     3601 cggggcaagg tgttctggga caattactgg gaaatgcgcc gcgaatacgt gcccaaaggc
     3661 ctgcgctgga tagtcgatta cgactgcaaa aggccgcgtc tcctgttgat gcagaattgc
     3721 agcaacgtta cgctgagcga agccaaattc atagaagccg gtttctggac tgtgcatatt
     3781 ttgtattcca cacaagtgac catcaagaat ctgaccatcg acaacaacaa tgccggcagg
     3841 ggtcccagca ccgacggcat cgacatcgat tcttccaacc ggatactgat tgaagattgc
     3901 gacatcagtt gcaacgacga taatttctgc ctcaaagccg gccgcgatgc cgacggcctg
     3961 cgggtgaacc gtcccaccga atacgtggtg atccgcaatt gcattgcccg ctacggtgac
     4021 ggcctcttca cctgcggcag cgaaacctcg ggcggcatcc gcaacgtggt ggcctacaac
     4081 ctcgaagcca taggcaccaa gataggtttg cgctttaagt cggcctatac ccgtggcggc
     4141 gtagtcgagg atatttacct ggccaacatc aaaatgagag gcctcagaga gcctttacag
     4201 atcaacctca actggaaccc cagctacagc tattccagcc tgcccgaggc ctacgacatc
     4261 aacgaagtgc ccccgcactg gaaggtaatg ctcaccccgg tcgatccacc ggaactgggt
     4321 atgcctaaat ttcgcaatgt tcattttacc gatatcaagg ccgacagttg ctcggtggct
     4381 atcacttgtg aaggagccga aaattcgacc atcgatcagt tttacatgaa caacgtacac
     4441 atcaccggag ccaaagccgg ccgtatatat tgggccaaag actggaaatt gaaaaatgtg
     4501 tggataggca gcgaaaaagg cgagatgccc gacatacaaa acaccgtcaa tgtgcccttg
     4561 ctccaggaat aatttttcta atagaataaa aacacagacc tcaacagatg ccaaacatga
     4621 aaaatctttt actgactgcc accctggcgc tcggaatttt tgtgtcctgt cagcaggcgg
     4681 gacaggagtc tatcgatcgc aaagctctgg tcgacaggaa taaagtacag gtgaacgctt
     4741 tcgattccct ggcctctcta tcgttgggta atggtaattt cgcttttacc gtcgacatta
     4801 ccggcctcca gacttttccc gaacgataca gcaaaggagt gcctctgggc acacagtcgc
     4861 aatggggctg gcatagtttc cccaacactc aaaaataccg tttcgaagaa acgctcaggg
     4921 cctacaattt cagaggcaaa gaagaattgt atgcggtgca gttcaacgaa aaaggtcgtc
     4981 cccaggctgc tgcagattat ttcaggatca atccccatcg ccttcatttg ggcgtggtag
     5041 gcctggaatt gcgcaatcct cagggggaga ttatgcggcc cgaagatttg agtgaaatca
     5101 atcaggagct cgatttatgg aacgggacaa taaattcctt attcaaagcc ggcgaattca
     5161 gcgtgcatac tcaatcttac tgtcacccgg agatggacat gatggcttct tccatagaat
     5221 cgtccatgtt ccttagcggc aacctccgcc tgaggctggc atttccttac cccagcggca
     5281 accacagcga cgacgcctgc gactggagca gccccggcaa acacagtacc aaagtgttgg
     5341 ctcaaaagcc cggagctgtc ttgctggagc gtcgtttgga caacgacagc tattacgtcc
     5401 tgttgtgttg gaaggagcag gccgaattta tctccggccc cgcggcgcac catttttacc
     5461 tgcaacccgc cggcgaaaag ctgagtttta cctgcctttt cctcgaacgc aatcctctgc
     5521 tcgattcttc cgaattaccg gcctgccccg aatcggagca ggtggctcag gcatcctcgg
     5581 attattggaa tcaattttgg ctcagcggag gcgcagtcga cttttcggct tgtaccgatc
     5641 cccgtgcgcc ggagctggaa cgccgggtgg tgctttcgca atacttgatg gctattcaat
     5701 gtgccggtgt ttatccgcct caggaaagcg ggctcacgta taatacctgg tacggtaaat
     5761 tccatttgga aatgcattgg tggcacgggg tgcattttgt tctctgggga cgtcccgaat
     5821 tgttggaacg cagtctcgac tggtaccgta cagcagaagc aaatgccgct aatattgctc
     5881 aacgtcaggg cttcaaggga gtgcgctgga tgaaaatgac cgatccttcg gccatagaag
     5941 ctccttcgaa ggtgggctcc tttttgatct ggcaacaacc gcatatcatt tatatggccg
     6001 aattgctgtt ccgtcaccaa aaggacagtt ccgtcctggc cgactacagc gacctggtgt
     6061 ttaaaacagc cgaatttatg gccgactttg ccgattacga cggaatggga gaccgctata
     6121 tcctgcaagg ggccattccg gcgcaggaaa ccctgagggc ttcggaaacc gtcaattccc
     6181 cctttgaact ctcttactgg cattatgcgt tgagtgtggc tcagcaatgg cgtttgcgtc
     6241 tgggtttgcc taaaaatatg gagtgggaga tcgtgctgga caagctttcg cctctggcgc
     6301 acaaagacgg actgtatctg gccgccgaaa cagctttgga cacctacatc gacacccgtt
     6361 acacttccga ccacatggcg gtgctgggag ctctggggat gatgcccaaa tcgagactgg
     6421 tacgcgaaga tattatgacc aacacgttca actggatttg ggacaactgg aactggggca
     6481 aaacctgggg ctgggattac cccatgacgg ccatgtgtgc cgcccgtatc ggcctgcccg
     6541 aaaaggctgt ggatgccttg cttatggata ggcgcaccaa tacctacctg gtcaacggtc
     6601 acaattatca ggacggccgc ttgcgggtgt atctacccgg aaacggaggc ttgcttacgg
     6661 ccgtagccat gatgtgtgcc ggctgggacg gtagcgaggg taaaaatccc ggtttcccca
     6721 acaacggcca atggaaggtg aaatgggaag gcctggatgt gatgccttga tcagaaaaag
     6781 taattatgaa gtggatattg cgcgtttttc cggccttgtt tttgattttt tctgccggtt
     6841 taacagaggt aaatgcccaa aataaactgg cctttcccgg cgccgaaggt tttggccgtt
     6901 ttgccaccgg aggcagggga ggcagtattt accacgtaac caacctcaac gactcgggcg
     6961 aaggttccct gcgtgatgcc gtcagccggc ccaatcgaat cgttgtcttc gatgtttcgg
     7021 gcgtgattcg tttgcaatcg gctttggttt tttccaagaa ccttactgtg ttgggtcaaa
     7081 ctgctccggg cggcggtata caggtgtatg gcgatcaggt gtcgttttcg ggcgctgata
     7141 acatcatcgt gcgttatctg cgtattcgta tggggataga cggcaccgac ggcaaagacg
     7201 ccgcgggcgt tgccaacggc aaaaacatga tcttcgatca tatgtcggtg ctttggggca
     7261 aagacgagaa cttttcggtc tcttggggta ataggggtac ggaaccaacg aatattacca
     7321 ttcaaaattc cattattggc cagggtttgc agccgcattc ctgtggcggt ctgattcaga
     7381 cccgaggagg tgtcactctg tttcgtaacc tcttcatcga aaataatacc cgtaatccca
     7441 aagtaaaggg actgaatcaa tacgtcaaca acgtggtcta caactggggc gacggctgct
     7501 acatcatggg cggttccgaa gggccctcct gggcgcacat cgaaaataat tattttataa
     7561 acgggccctg ggaaggagca acgcctgtgt ttacaagggg taatgtcaat tttacttttt
     7621 acggggccgg caattaccac gatgcaaata ggaacgggct gctcgacggt agtctgatga
     7681 cggccgatca atattctgct tcgataccgg tgtccgattg ggctacctgg gacaattcta
     7741 ctgccagacc tcaaccgcat ccggaaattt tgagtatgac gactgctcag gaagccctgg
     7801 cctggattat cgacagtgta gggccctgtc tgccggtgcg cgacgaggta gatcaatacc
     7861 taatcgatga attggtttct tacggaacca aaggcactgc caacggtatt ataagcgaaa
     7921 agaccttgcc gcacaaaggt acaggtaccc tgcgcggagg ttatagaccc ccggataccg
     7981 accaagacgg aattcccgat gcctgggaaa cggccaacgg actcaatccg aacgatcctt
     8041 cggatgctgc tgccattgcc gccaacggct acagcaacat cgaaaattat tctttcagta
     8101 taaccgcctg catcaaatcg cctttgtaat cgagactaaa cacttgttct cgaagcgata
     8161 tgacagaatt taggcgattt atatatttaa aaaggttttg ttgtgaacgt agtctttcag
     8221 caaattgtat atgctctggt ggcccttacg gtgctgagcg tcgtgctggt gattatttcg
     8281 gaaaaccgca atccggtcaa aagtgtagcc tggatactgt tgctcatttt tttgcccttg
     8341 ctgggcctga tcgcttattt ccttatagga caagatttta ccaagaagta tgcctataaa
     8401 agaaagaaac acagaggtaa agatcaggct tttctgatca agagcgacca ggtgctggat
     8461 gaacaggagt atccccgggc ttttaccgct ccggtaaaat tgctcaaaaa catccagaac
     8521 gcccttctga taggcggcaa cgacgtgaag tactttactc ttgccgccga caaataccgg
     8581 caaatgctgg ccgatatgga agaagccaag cgttttattc acctggaata ttacgcactt
     8641 gagaacggcc tctggggcga tgctcttaag gatttactca tacgtaaatc ggccagaggg
     8701 gtggaggtca gaatcattta cgacgactgg ggttgttgga gaaccaaaaa aagctacttc
     8761 aaagagctga tagataatgg agtacaagta cgtgtttttt caaaagttcg tatactgccc
     8821 ttgacggccc gtctcaacta ccgtgatcac cgcaaattga tcatcatcga cggtcttatc
     8881 ggatatattg gcggaatgaa tatcagcgac cgctatgtga atggtttcaa ctggggctgt
     8941 tgggccgata cccattgccg gattgccggt cccggggtgg tgcagttgca atcggtcttt
     9001 ttgtctaact ggcagcgtca gtcgggcgaa gtcttgtggg aggacgagta ttttcctttg
     9061 ccggcttctt gcggcgacaa cgctttgcag gtggtggcca gcggaccggg tagcctcgat
     9121 cccgaaatct tgcacgttat gattcaggct ttttacagcg ctgaaaaata catttatgtt
     9181 cagactccct attttattcc cccggaaccg ctggcaggcg ccctgcaggc ggcagctatc
     9241 cgtggtctgg acgtacgtct gatgatatcc aaacgttccg atctgcctct ggtgcaaatg
     9301 gcttcgcgtt cttatattcg tgagatgctc gagcgtggaa taaaggtcta tttctacacc
     9361 cggggctttt tgcactccaa aatgatggtg gtcgacgggg aattgagcat tatgggctcg
     9421 gccaacgccg atatgcggag tttcgaagat aacctcgaag tgcaggcctt tatctacggc
     9481 cgatctgccg ctctcagggc tcaggagata tttctcgaat atcagcagga ttgtgaaaaa
     9541 attaacctgg aacgatggaa aaaacgttcc aggttaaaac ggtttgtgga gtcctttatg
     9601 cgtctgtttg ctcccttgct ttaagactca agccggctta gggttggtag tgcttgacga
     9661 tccggctgct tttgccgctt tggagtttga tcaggtaaat gcctttggcc aggtttaact
     9721 ccttgacatc cttttcccat tcggtggcct tgctttccag gctggcgacg gtgagtccgt
     9781 tgatgttgac cacctgtatg tcgagcattt cgtcccagct attgagctgc aggccgttgg
     9841 catcggttac gctgagtttg cctctgccgg taaagagttc gctggtgtcc caggccaggt
     9901 tggccggcaa ttccggcaag accagttcgg gaatactact gctgaagcta gtgcaggacc
     9961 aaagctcaaa actgtcgccc agagaaggtg tgtatccctc tttgagcagt atgcgcagcg
    10021 tgcctttcag agtcattaca ttcaaacctt ttatactcga atgagaggtg gccgagttga
    10081 gccggaattc cagcactcct ccggggtttt gataaatgga tccgtccact ttcagttcgc
    10141 ccacgtaata ggctcccggt atgaacaggc ctccggagtt tatgtaaacg atgttgttgt
    10201 ctataaatcc tctgccggtt agcgtgcctc cgttgttgat gttcagccgg ccggttcctg
    10261 tggcgctgac ggtagcgttg gcgttgttta ccctgaggat gcctccgttg atgttggtgt
    10321 ctccggtata gttgttggct ccggaaagaa gcagggtgcc ggtacccact ttggtcaaag
    10381 cggaagcgat ggtgccgctg aagctgttgg tggtgttcag gtaaccgatt tcccaggcgt
    10441 tggaaccgcc cagggttccg ttgccgctga cggctccgag cttaaagttt ccgctctggt
    10501 tggtcaccgt ggtgccggcg gctacgtcga gcgtggcctt gggtaagccg tagctgtttt
    10561 tgaaatcaaa agaaggagta tagctcgaat tggccttact ggtggccttg accactccct
    10621 cgaaagccga ccaattgcca caaagataat tcctgacata gggggcgctc agggtcagtg
    10681 tgccgctgcc gtgcagggat ccgtggtatt cgcagcgtcc gtccaggtaa atggtagccg
    10741 attttccggc aggtaccact atattccagt tggctctgct gtaggaactg tttccatcca
    10801 ggcagatcac cactccgccg tttagaacca gagtgtcgcc aaaatagccg ttgattaaat
    10861 tgggctcttc cagcactttg agggtgccgc cttcgaggat ggtttttttg tgcgtgttca
    10921 ctgcagttac ttcgagggtg ccgttgcccc gtttaatcag ggtgccgccg gaaataactc
    10981 ctttcatggt cagggtgttg gtaatgtcca gacaaccacc ttcgggtccc agatacagtg
    11041 ggctttccat gctgatgttg ccggtggttt tcagtatcgc gccgttttga atggtcaggt
    11101 tgttcgacac cgaactcagg gcgcccagtg agccggctcc ctgcgaagtg gccagcgaac
    11161 tgacttctac cgttccccct ttgataaggg ttttgccggt atagggattg gtattgagca
    11221 ggctgagttt accctggcct tctttgatga gcgaggagct tccgcctatg ccgccgttac
    11281 cggttatggc atagtttaaa tcgccttgaa ttctaacctc cgaaggaatg acttcttcgg
    11341 tgatatttac cgctctggcg ccggtgtttt ggtcgaagac cacggtgtcg ttggtaacaa
    11401 attccaccgt atcttgtccg atgtaaaagt ttctcgtatt gttcagattc cagatcccct
    11461 gatcgaattt accatgccag tagaccaatt gtgccgcgcg ttgttcgtca acgtgcaaaa
    11521 acaggctgtc gttttggttt agcaccgagc aggcgacgcc gggcaggcct tcgatgacga
    11581 ttttgttcag ttcggccgta acgatgcctt cacatacggc aaatagatag tcgccgggct
    11641 gtggtttaaa atccggtcta ggttttacaa aacggaaaac agcgccgtcg ctcagctcca
    11701 gattttcttg aataatcaga gtgtcgcagg tgaaatcagc gtcggaaaga ttgaattcca
    11761 ctacggctcc cttgtccatt tttatggact tcaggcggag gccgctttgt ttgccggaac
    11821 ctcccgggcg caaaatggcc ccgtactcca tttgcaggcc tccttccaag gagttatagg
    11881 cgtccagctc tgcgaagcga cgcagcaaca gagggccggc taagctgcct ttgagtgtaa
    11941 gcaagccgtc ccagacttcg gtattcccgc tgtaggtctg cgctccttcg atggtgaggt
    12001 tgccgagtcc ctgtttgtgc agggtcatgg ggccttgcag ttcccctgtt ccgtagagcc
    12061 tgtagtctcc gggtgaatta acattcacag aggcgggact gaccttgctc ctaagctcga
    12121 cttctgctcc ctgggtgttg gccagcatga gctcttttcc ctgatggtca ccgctgataa
    12181 aaccgtcgac ttccgtttta ccgttgctta tccaaggggg agggggagcg gtaatacctt
    12241 ccctttcgcc caaaaagaag ctggtatgcg ggggctggtt gtagccgcac atttgccagc
    12301 atatggcctg acggtactga tggtcgtgca tcagggtata tatgggccag ggcgatggaa
    12361 ccacgctggt atagattctt aagtacttgt tgtcggtgct gcgcaagacc atttcttcgc
    12421 gccagtcgcc aaacaggtct gcctgcaggc agggatttcc cttggtgtag ttgttggtgg
    12481 ctgtgccggt ggcagtgaat atattgctca cactaccctt gttgtattta aacactgccg
    12541 gtgttccgta accgtagccg ccggtaacat cgtttacatt agtgtaatcg aaggtctctc
    12601 tcagcaggtc tccgtcccag tagatggcaa agttttgtgt tcccccggaa ctgccaacca
    12661 attctctggt ggtcgcactc cacatttttc cgtctgccca tagttccgct ccgggataat
    12721 cgtccgtaac gttggcagcc atacaacggc cgcaatctct gcccctaacg tatttgataa
    12781 aggtttctgt ggtggcgccg tcgcggaaag cagctcccca gtggggagaa gattccaggc
    12841 aggcaaaaat ctccagcccc cggtggtaag gatcaaaatc gctgacgtgc atggcatcgc
    12901 cgtgaccgta accggtagag tacaggcccc tgccgttgtc gtctaccgtc atcgatccgt
    12961 aaacgatttc atcgcggccg tcgccgtcaa cgtcggctat ggtgtagttg tgattgccct
    13021 ggtaagcgta agccgaccat aaactgttgt tggtatcaaa gtccgacagc cagcgttttt
    13081 gtaatttctt gtcgaccacg tcgtaagcgc gcattactat gcgggtataa atgcctctgg
    13141 tggtaagtat gctgggacgg cgtccgtcca gatagggagc gccgaaaaag aatttattgg
    13201 cgcgatggcc gtaaccgtcg ccccagtcat tgacgttgcc ccgggcaatg taatcgactt
    13261 tatcgagcag ctcgccggtt tctccatcaa aaaggcataa gaactccggc ccctctatca
    13321 taaactgcat attgggagac tggttgacat aggaacggta attggccgtc gggctgccta
    13381 taacattacc cttgccgtct atggttccgt cggccgcccg catgataact tcggctttgc
    13441 cgtcttcgtc ccagtcgaag gcagcaatgt tggtttcaac gtgtccgctg ctcaacaggt
    13501 tggggccgat atcaatagcc caaagcaggg ttccgtccag tttataggct tcgaaatagc
    13561 tgaaggcact gtcgttggct accgaaaagt cgctgttgat gcgtttgaca ataatttcgt
    13621 attgtccgtc gccgtcgaga tcggcagccg tagcatcgtt cagttcgtag agtgggccgt
    13681 agtcgatgcc gtttttgatg atctggcgca tggggatttc caggtattgt ttgttccaga
    13741 ccgcgtaatt gcccggttca gggctttcaa caccttcttt gaccaatccg agggtataag
    13801 tcgaagcatt gctgcctccc tcgtccaggt agttcgaaac actcagcggt tctgtgttca
    13861 gtttgacgcc atttcgatat aagttgtatt gggtattgtt ccattccgaa cccggaatgc
    13921 gccaggaaac aaaaacacca ttggaaactt tcactgccgt cagtccccgg tcgagatttt
    13981 ccataagccg ctggccgtac gaaaacatca cagcgaccac gctcaaacag aggaatagaa
    14041 gaaacttttt catcttctaa tataaagttg ctgataattt tattccagat ccagtttata
    14101 gatttcggaa ccggccagca ggaagcagcc aaggccgtaa tcgtcaaaat cgggctctat
    14161 gtcgtaaccc accggttgtc cgtctttggg ttccttgccc gttccctgca catagcccag
    14221 gaagccgttg gggtgtacgg cttcttccag tgctttccag cctttaagca ctgccggcag
    14281 gtagattttg gccggcaaaa taccgtggcg tatgccccag gtcatgccaa aaacgaaaag
    14341 agctgttccc gaagtctctt tgcctccgaa atgtccggga tcgtgcaggc tgatattcca
    14401 gtagccgtct tcgcgctggc aggccagcag agctttggac atagccttga aatctttcag
    14461 gtattgatcc ctgtgttttt cgtcggcggg agcgatttcc atatacctgg ccagggc
//
LOCUS       DAOSTD010000040        14421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_4272_length_14421_cov_13.462063, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000040 DAOSTD010000000
VERSION     DAOSTD010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14421
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4272_length_14421_cov_13.462063"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..>2242)
                     /locus_tag="PLI37_04155"
     CDS             complement(189..>2242)
                     /locus_tag="PLI37_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOQ58242.1"
                     /translation="GSTNAKKGDFRLFGLEGIPYELTITEQSITLKIKSSREPASVLW
                     TGNENNTWDFATPNFNWQNGASIFVPGDSVRFTDAAVSKQIVINETMPVGQLMFENEE
                     AFSLSGRGVVSGEGGLSKSGGGLLSIQNTDNSFTGPVIIENGILEVASLKDGGLPSSI
                     GASSNSGQNWIMRNATLQTRSQMSTNRHMQVVDTLTINNPASNNSVLISGNISGANTT
                     LVVKGKGSLTLQGSNTFGRVILKDGLLLLGSSHANRYSMGSAKIRLEGGIFRMHNINT
                     TSDTDNFRNEIEVPEGKTAYWDLPSRWGITSKLTGSGRLVVYIPYVRSDLNGDWSAFS
                     GRIDFVGRDVRLNNAAARNLPLAEVNLGDGTYLMAASNGSSESSTAQTFTLGALSGTG
                     TISGRNSLVIGSKNTSTVFSGTISSGGGRLTKQGSGELDLRGANEYTGGTIVQQGSLI
                     VSNSSGSATGTGNVTVRSGATLTGRGSIAGTVQVLEGGILAAGSPTNVGSTLKLGRNL
                     TMNPGSRMVFKQRLLYCDKLTVDGNISLNGTLELQIQGADLKEGRSLALFTAGGTVSG
                     AFEAIEPAAPGEGLEWDLSRINEGILAVQAASALPSATSAEFRLIQSDNLPIYRINCE
                     AATDPIKVEILSLTGKLMENLESPAPDCLVDLNNYPAGLYLLRVSANHKTKVFKIVKK
                     E"
     assembly_gap    2243..2342
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2343..4753)
                     /locus_tag="PLI37_04160"
     CDS             complement(<2343..4753)
                     /locus_tag="PLI37_04160"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58243.1"
                     /translation="MNQSNTLRRATNLPHTAHSPGTAESQLTADPPQATDPTQTTGRG
                     LIAGKTLLCLVFILLFLPGTLAQRRVDSPDRGIVAIPKSANTIYLSWRHFATDPDEIA
                     YNLYYKTSAEGALTKLNSNPVTGSTNYTANLSTSASAYTFVVKSVLEGVEKDEAGSFT
                     LPQNSGIHRIVRDFDFEPLPDGHPKMAMKFCWPADLNGDGAYDYVIDRQDYGASMMED
                     GGEATAGYPYPKVEAYSSEGVFLWRIDMGPNIKICSGHDDMVTAYDMDGDGKAEVLMA
                     VSEGTTFADGTVIKGSNGQVTDYRTLAGPAPQWIAVVDGETGLLIDKVDLPFYAELRN
                     QRTDNWKDIGGHFIINYLDGIRPSLVYMYKNRAENGWFYGAHAAFSYRNNKLVKDWAY
                     RFWPNQAEFHQVRAADVDGDGRDNFVEGGYVLKHDGTILNRHQDVVHGDRHCLADIDP
                     DRPGLEHFVIQQNNPKTLGMGLFDAASGAIIKGNYQSAVGDVGRGICGAFDATRRGMQ
                     YWSTMNGNAMYDCKGDLIEGAAGTFPSEALWWGPDLARWEISGIGSGGPNLAFHVYNP
                     SNKSFYRDQPNLYNEYIPYYFKSINAGRAAFWGDMLGDWREELICVRSDWTGFVVLST
                     WEQTNHRIYHLMQNPAYRLQTTAKGYYQTPDVDFYMAADMPKPPIAPVQQADLYYSGS
                     GWMDREEQAADYADGKSVMFDLRGGNDTFNIEGNFSPSVLYLMNPKGADYTLEGSGKI
                     TGGTRLIKSMQGDVVLRGQHDYTGSTRISEGRLWLDGSLDSPVQIDARGVLGGRGVLK
                     GGVIL"
     gene            4951..6174
                     /gene="pepT"
                     /locus_tag="PLI37_04165"
     CDS             4951..6174
                     /gene="pepT"
                     /locus_tag="PLI37_04165"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOQ58244.1"
                     /translation="MQLIDRFLRYVKMDTQSDDATNLVPSTPSQMEFAKKLAEELRQI
                     GLQEVSLDKNAYVMATLPANTDKVRPVIGFIAHMDTSPDMSGRKVQPQVVRKYNGHDI
                     VLNAKENIVLSPQQFPELLHYIGQDLICTNGLTLLGADDKAGIAEIVTAMEYLLAHPE
                     IKHGKIRLGFTPDEEIGRGADHFDVKAFGADFAYTLDGGEIGELEFENFNAASAKVRI
                     KGLNVHPGYAKHKMRNACRIAQQFVSMLPRHETPEHTEGYEGFYHLTAMQGTVEEATL
                     DYIIRDHDRDRFERRKKEMQHLVNKINQEFGEGTATLTLKDQYYNMREKIDPDNMHVV
                     QLAIDAMKAVGVEPKIKPIRGGTDGARLSYMGLPCPNIFAGGLNFHGRYEFIPIPSMR
                     KAMEVVIKIAEINCN"
     gene            6187..7272
                     /gene="gcvT"
                     /locus_tag="PLI37_04170"
     CDS             6187..7272
                     /gene="gcvT"
                     /locus_tag="PLI37_04170"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOQ58245.1"
                     /translation="MKTTPFTDIHLGLKAKMHEFAGYNMPIEYTGILPEHLCVCEGVG
                     VFDVSHMGEFAVSGPRALDFLQQICSNDVSVLTDGQAQYNYFPNEQGGIVDDLLVYRI
                     NKNEYLLVVNASNIEKDWDWCCSHNQSGAKLENLSNRTAQLAIQGPKALDVLQKLSPV
                     DLKAIPYYHFTYGKMAGIDQVLISNTGYTGAGGFELYFKPEDGLKLWEAIFEAGAEYG
                     IQPIGLGARDTLRLEMGFCLYGNEIDDTTSPFEAGLGWITKFLPGKNFPGRAILEKQK
                     QEGVNQKLCGLEMIDKGIARQGYEVVDAQGEAIGRVTSGTISPMLKKGIALAYVKTPF
                     TQPGTEVFVRVRNRDLKAKVVKLPFRK"
     gene            complement(7429..8016)
                     /locus_tag="PLI37_04175"
     CDS             complement(7429..8016)
                     /locus_tag="PLI37_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOQ58246.1"
                     /translation="MRIQDLIIKHNALKPEKGRILIAEPFLAGEPFERSVVLMCESGP
                     NGHLGFVLNKPIGFNLDEVVSTNERIPKIPIYLGGPVAAKRLFYVHSLGSEIKGSIAV
                     SDELYMDGDLNQLLSLLALDEEAKNKIRFFVGYSGWVEGQLEAEIRDDSWLVGKIGLK
                     EIFQSRSSTLWKKCLRQLGEKYKVWSMYPLIPSLN"
     gene            complement(8033..9358)
                     /locus_tag="PLI37_04180"
     CDS             complement(8033..9358)
                     /locus_tag="PLI37_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOQ58247.1"
                     /translation="MSQQRDTPIDSVIVQNILDEYNLPDFGKATIREVVSMVNQAEKR
                     SGKRYVRMEMGVPGLPPSKVGVEAEIAALQKGVASIYPMLDGHPELKAEAARFVKAFI
                     DVDVPAECCVPVTGSMQGSFTSFITCSQCNRSKDRLLFIDPGFPVQKQQLVVLNTAYD
                     SFDVYHYRGSALREKLESILSLGKTSALIYSNPNNPAWFCLKDSELKIIGELAEKYDV
                     IVIEDLAYFAMDFRQDLGRPFEPPFQPSVAKYTDNYILLISASKAFSYAGQRIGVAVL
                     SEKLYKRPYADLKKQYGVCEFGNVFVNRVLYSLSSGTSHSAQYAMAAMMKAASDGEYR
                     FLDEVREYGRRAEKVKKLMLGNQFEIVYDNDLGEALADGFYFTVRYPGMSGAELMKEL
                     LFYGISAISLETTGSDQQGLRICTSFIKDDQYEELESRLKKFRADHPLN"
     gene            9541..9614
                     /locus_tag="PLI37_04185"
     tRNA            9541..9614
                     /locus_tag="PLI37_04185"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9575..9577,aa:Asp,seq:gtc)
     gene            complement(9743..9865)
                     /locus_tag="PLI37_04190"
                     /pseudo
     CDS             complement(9743..9865)
                     /locus_tag="PLI37_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(9925..11109)
                     /locus_tag="PLI37_04195"
     CDS             complement(9925..11109)
                     /locus_tag="PLI37_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ58248.1"
                     /translation="MFYRSYLETVKKRIQEPRKFIQVILGPRQVGKTTMITQLLSQLS
                     VPNLYESADAIPVTNSVWLTQLWEAARLQIKVSGATEYLIVIDEIQKIDNWSEIVKQQ
                     WDKDTRERTNIKVILLGSSRLLIQQGLTESLAGRFETIYLGHWTYTEMQEAFGWSIEQ
                     YVYFGGYPGSATLIDDEERWKNYIKDSLIETSISKDILMLTRVNKPALLKRLFELGSL
                     YSGQVLSYTKIIGQLQDAGNTTTLANYLKLLSDCGLLGGLDKYAGDIIRKRGSSPKFQ
                     VYNNALITSQANDAYEKAIINPELWGRLVESSVGTHLINNSIPERYNLYYWREGNHEV
                     DFILEKGDKVIGLEVKSGMKAENTGMGVFAEKFHPEKVLLVGTGGIPYEEFLKINPRE
                     LF"
     gene            complement(11273..12550)
                     /locus_tag="PLI37_04200"
     CDS             complement(11273..12550)
                     /locus_tag="PLI37_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ58249.1"
                     /translation="MVEIINSIRKYNFWDNNPIDLGYPRTFYTDKIGQYVGNKLVKVL
                     VGQRRAGKSYILRQIASKLLSGGVKSENILYINKEYIELSTLRSAIELEQLYKAYREV
                     LNPSGKVYIFIDEIQYIEEWERFVNSHSQDFAEPCELFISGSNSKLLSGELATMLSGR
                     YVEFEVLPYSYTEFCGVTQQEKNSESYKEFLQDGALPELFNLPNDEMKQNYVSSIKDT
                     VMLRDIVARYNVKDVKLLDDLFVYLVNSASNLISITNIIHYFASKKRKTNYETLSSYI
                     SYLESSFLVHRVERYNIKGKDTISGNSKYYMNDLSYRNYLYTGFGYGIGYLLENAVYL
                     SLRRAGYQIYVGTIKNTEVDFVAIKGDRRLYLQVTLSLTEEHTIEREYRSLKLIDDNF
                     DKYVVSMDDYNIPTNEGIEHISAWKLEDLLNSR"
     gene            complement(12582..12752)
                     /locus_tag="PLI37_04205"
     CDS             complement(12582..12752)
                     /locus_tag="PLI37_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58250.1"
                     /translation="MLIQRTAEKELQSLARNYKAMKPYIADDIKACTMSKFNNWWENK
                     QNKKIDNIFKYI"
     gene            12824..>13110
                     /locus_tag="PLI37_04210"
     CDS             12824..>13110
                     /locus_tag="PLI37_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58251.1"
                     /translation="MFFTFCPYSSWKDRAMLQHEKPLLTALYLNKIGHKQYKSFCLSI
                     ELQRLKFRLSLLQAYVNRNERPDLKAVDKKVETAFEEYNRQIEAEAERLAAA"
     assembly_gap    13111..13210
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13211..13404
                     /locus_tag="PLI37_04215"
     CDS             <13211..13404
                     /locus_tag="PLI37_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOQ58252.1"
                     /translation="FEEYNRQIEAEAERLAAANRFLSGNFLTSENSKKLRELYYFIVK
                     RLHPDINADLSDNMKDLFI"
     gene            13747..>14421
                     /locus_tag="PLI37_04220"
     CDS             13747..>14421
                     /locus_tag="PLI37_04220"
                     /inference="COORDINATES: protein motif:HMM:NF036560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas6"
                     /protein_id="HOQ58253.1"
                     /translation="MGNTKIVTIRFKNIISPTDIEAFRGAVVNALKDKDILFHNHNLT
                     DKGFRYSYPLIQYKRINRRAAIFCLEEGTESIGKFFLDSDLTLNLNGKIHQFEVESVK
                     AHKHLIQIWNSNIRYTIRKWLALNQENYELFDTLESVAEKSAFLENILKANILSFAKG
                     LNVFFEKQVECKITRLSEPRITLYKNVKMTTFDAEFLTNVSIPDYAGLGKGVSVGYGV
                     TVRKKEK"
BASE COUNT         3585 a         3346 c         3364 g         3926 t
ORIGIN      
        1 cagagcacgt gatttgtaat ctcggggtcg ttggttcgaa tccgacaaga ggctctcact
       61 tcttcccgat gattgcctga ctttgtcggc agaaattgaa tgggcagaaa aaagaggatg
      121 gctttacagt caccctctcg gttctgttga agttggagtt tgctctattc tactgtaaac
      181 tataatctct attccttctt tacaattttg aatactttgg tcttatggtt ggcactgacc
      241 ctgagcaagt acaagcctgc cgggtaattg ttcagatcga ccaggcagtc gggcgcaggg
      301 ctttcgaggt tttccatgag tttgccggtc aggctgagaa tttccacctt gattgggtct
      361 gtggccgctt cgcagttgat gcgatagata ggaaggttgt cgctttgaat aagccggaat
      421 tcagcgcttg ttgccgaggg tagggctgag gcagcctgta ctgccaggat accttcgttg
      481 atacggctga ggtcccactc caaaccttcg ccgggagcgg caggttcaat ggcttcgaaa
      541 gcgccgctta ctgtaccgcc ggccgtgaag agcgctaaac tgcggccttc tttcaggtct
      601 gctccctgaa tttgcagttc cagggttccg ttcaggctga tgtttccgtc aacagtcagt
      661 ttgtcgcagt agagcaggcg ttgcttaaat accatgcggc tgcccgggtt catagtcagg
      721 tttctgccta atttcaatgt gctgcctacg ttggtggggg aacctgctgc gaggatgcct
      781 ccttccagca cctgtaccgt accggcgatg ctgccccgtc cggtcagtgt ggctccggaa
      841 cgcaccgtga cgttgcccgt gccggtggca cttcctgagc tattgctcac gatcaggctg
      901 ccttgttgaa caatggtacc gccggtgtat tcgttggctc ctcgcagatc gagttcgccg
      961 ctgccttgtt tggtaaggcg tccgcctccg ctgctgatgg ttccgctgaa aacggtggag
     1021 gtatttttac tgccgatgac cagactgttg cggcccgaaa tggttcccgt gccgctcaat
     1081 gctcccaaag taaatgtttg agccgtagaa gattcgctgc tgccgttgga agctgccatt
     1141 aggtaagtgc cgtcgcccag attgacttct gccaggggca ggttgcgagc tgcggcgttg
     1201 ttgaggcgaa catcacggcc gacaaaatca atgcggccgc taaaggcaga ccagtcgccg
     1261 ttgaggtcgg aacgcacata gggaatgtaa acgaccaggc gccccgatcc cgtaagtttt
     1321 gacgtgatgc cccagcgcga gggcaaatcc cagtaggcgg ttttcccttc gggaacttcg
     1381 atttcgtttc taaaattgtc ggtgtcggaa gttgtattta tgttgtgcat acggaaaata
     1441 ccgccctcga ggcgtatttt ggcggaaccc atggaatagc ggttggcatg ggaggaaccc
     1501 agcaaaagca ggccgtcttt cagaattacc cggccgaaag tattgctgcc ctgcaaagta
     1561 aggctgcctt tccctttaac aaccagggta gtgttggctc cgctgatgtt gccgctgatc
     1621 agcacactgt tgttggatgc gggattgttt attgtcaggg tgtctaccac ctgcatatgc
     1681 cggttggtcg acatctgcga cctggtttgc agggtggcgt tgcgcataat ccagttttgg
     1741 ccgctgttgg aagaggcccc tatggaactg ggcaggccac cgtctttgag agaggccact
     1801 tcgagtatgc cgttctctat aatcacaggc ccggtaaaac tgttgtcggt gttttgtatg
     1861 gacaacaggc ccccgccgct tttgctcagt ccgccctcgc cgctgacgac tccccggcca
     1921 ctcagactga aggcttcttc gttttcgaac atcagttgcc ccaccggcat ggtttcgttg
     1981 atcacaattt gtttggaaac agccgcgtct gtaaagcgga cgctgtcgcc gggcacaaag
     2041 atcgaagctc cgttttgcca gttgaaattg ggagtggcaa agtcccaggt gttgttttcg
     2101 ttacccgtcc acagaaccga ggccggttcg cgcgaagatt tgattttcag cgtaatgctt
     2161 tgttctgtta tagtaagttc gtaaggaatg ccctccagtc cgaatagcct gaaatctccc
     2221 tttttagcgt tggtgctgcc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nntccagaat tactcctcct tttagtactc cgcggcctcc caacaccccg cgggcatcga
     2401 tctgcacggg actgtccaaa ctgccgtcca gccagagcct gccttccgaa attctggtgc
     2461 ttcccgtgta atcgtgctgt ccgcgcaaaa cgacatcgcc ctgcatggat ttgataagcc
     2521 gggtgccgcc ggtaattttt ccgctgccct ccagcgtgta atcggcaccc ttaggattca
     2581 tgaggtagag caccgatggt gaaaagtttc cttcgatgtt gaaagtgtcg ttgcctcccc
     2641 tcaggtcgaa catgaccgac ttgccgtcgg catagtcggc cgcctgctcc tcacggtcca
     2701 tccagccgga ccccgagtag tagagatcgg cctgttgtac cggagctata ggtggtttgg
     2761 gcatgtcagc ggccatgtaa aaatccacgt cgggagtttg gtagtagccc ttggccgtag
     2821 tttgcaggcg gtaggccggg ttctgcatca ggtggtagat gcgatgattg gtttgctccc
     2881 aggtagatag caccacaaat ccggtccaat cgctccggac gcagatcagt tcctcgcgcc
     2941 agtcgcccag catgtcgccc caaaaggccg ccctgccggc gttgatcgat ttaaaataat
     3001 agggtatgta ttcgttgtat aaattgggtt gatcccggta aaacgattta ttcgacggat
     3061 tgtatacgtg aaaagccaga ttgggaccgc cgctgcctat gccgctgatt tcccagcgag
     3121 ccaggtcggg cccccaccat aaagcttcgg agggaaaagt gccggcggct ccttctatca
     3181 gatcgccctt gcagtcgtac atggcgttgc cgttcatggt cgaccagtac tgcatccccc
     3241 tgcgcgtagc gtcgaaggct ccgcaaattc cccggcccac atcgcccacg gccgattggt
     3301 agttaccttt gataattgca ccgctggctg catcgaacag gcccataccc aaggttttgg
     3361 gattgttttg ctggatgacg aaatgttcca ggccgggccg gtcggggtcg atgtcggcca
     3421 ggcaatgtcg gtcgccgtga acgacatcct ggtgacggtt caaaatcgtt ccgtcgtgct
     3481 tgagcacata acctccttct acaaaattat cgcgtccgtc gccgtccaca tcggctgccc
     3541 tgacctggtg aaattcagcc tgattaggcc agaagcgata ggcccagtcc ttgaccagct
     3601 tgttgttgcg atagctaaag gcggcgtggg ctccgtaaaa ccagccgttc tcagcccggt
     3661 ttttgtacat atataccaac gagggacgaa tgccgtcgag gtaattgata atgaaatggc
     3721 ctccgatgtc tttccagttg tcggtgcgtt gattgcgcag ttctgcataa aaaggcagat
     3781 ccactttgtc gatgagcagg ccggtttcgc cgtcgacgac ggctatccac tggggagcag
     3841 gtccggctaa ggtccggtag tccgtcacct gaccgttgct gcctttgatg actgttccat
     3901 cggcaaaggt ggtgccttcg ctgacggcca tcagcacttc ggctttgccg tcgccgtcca
     3961 tatcgtaggc agtcaccata tcgtcgtgtc ccgagcagat cttaatattg ggtcccatgt
     4021 cgatgcgcca caaaaagacg ccttcgctgc tgtaagcttc cactttggga tagggataac
     4081 cggcagtagc ctcgccgccg tcttccatca tggaagctcc gtaatcctga cggtctatca
     4141 cataatcgta cgcaccgtcg ccgtttaagt cggccggcca gcaaaatttc atagccattt
     4201 tgggatggcc atcgggcagg ggttcgaaat caaaatcccg gacgatgcgg tgtattccac
     4261 tgttttgagg cagagtaaag ctgcccgctt cgtctttttc cactccctcg aggacggatt
     4321 tgaccacaaa agtataggcg gacgcacttg tagacagatt agccgtatag ttggtgcttc
     4381 cggtaaccgg gttgctgttg agtttggtca atgccccttc ggccgaggtt ttgtagtaaa
     4441 ggttgtaggc tatttcgtcg ggatcggtag caaaatgccg ccatgacaga taaatagtgt
     4501 tggccgattt gggtatggcc acgataccgc gatccggaga gtccactctg cgttgagcca
     4561 atgtgcccgg caggaaaaga aggataaaaa caagacagag caaggttttc ccggcaatga
     4621 gtccccggcc ggtggtttgt gtaggatcgg tggcttgtgg tggatcggcg gtaagctgag
     4681 attcggcggt ccccggcgaa tgggctgtgt gcggcagatt ggtagctcgt cgaagagtat
     4741 tagattggtt catgtttatg caaattgtcc gtttatgttt tgtgaatcaa agtaaaaacc
     4801 aagtcgttat aaaaacatat cccggtcttt gtgaaagata aaatctgtcg caaagataat
     4861 taaaaagcga atttatatac atagacatac aaaacagggg agagcatcgg gaaattttga
     4921 ctatttttgc tcatcgcaaa aaagagtatt atgcaattga tagatcgatt tctgcgttac
     4981 gtcaagatgg atacccagtc tgacgacgct accaatttgg ttcccagtac cccttcacaa
     5041 atggaatttg caaaaaaact ggccgaagaa ctccgtcaaa tcggtttgca agaggtcagc
     5101 ctcgacaaaa atgcctatgt aatggccacc ttgccggcca ataccgataa agtccgcccg
     5161 gtgatcggtt tcattgccca tatggatacc agtcccgata tgtcgggacg taaggtacaa
     5221 cctcaggtcg ttcgaaaata caacggccat gacattgtcc ttaatgctaa agaaaacatt
     5281 gtgttgtctc cgcaacaatt tcccgagctt ctccattaca tcggtcagga tctcatctgc
     5341 actaacggac ttactttgct tggagcggac gacaaggccg gcattgccga aatcgtcacg
     5401 gctatggagt atttgctggc acatcccgaa attaaacacg gtaaaatccg actgggcttt
     5461 actcccgacg aagaaatagg ccggggtgcc gatcattttg acgtgaaagc tttcggcgcc
     5521 gattttgcct acaccctcga tggaggagaa ataggagagt tggaattcga aaatttcaac
     5581 gcggcttctg ccaaagtccg gatcaagggc ttaaacgtgc atccgggtta tgctaaacac
     5641 aaaatgcgca atgcctgtcg catagcccag caatttgtat ccatgttgcc ccggcacgaa
     5701 actcccgaac ataccgaggg ttacgaaggt ttttaccacc ttaccgccat gcagggcacc
     5761 gtggaagagg ccactctgga ctacatcata cgtgaccacg accgcgaccg ttttgaacgc
     5821 aggaaaaagg aaatgcagca cctggtcaat aaaatcaatc aggagtttgg tgaaggtact
     5881 gctactctga cactcaaaga ccagtattac aacatgcgcg aaaagatcga tcccgacaat
     5941 atgcatgtgg tgcagttggc catagatgcc atgaaggcag tgggcgtgga acctaagatt
     6001 aaacccatac ggggaggcac cgacggagcc cgcttgtctt atatgggttt gccttgtccc
     6061 aatatttttg ccggcggtct gaatttccac ggtcgctacg aatttatccc catcccctcc
     6121 atgcgcaaag ctatggaagt ggtgattaaa attgccgaaa tcaattgcaa ctaatttttt
     6181 ctcagcatga aaacaactcc ttttacagac atccacctgg ggctcaaagc caaaatgcat
     6241 gaatttgccg gttacaacat gcccattgaa tataccggta tcctgcccga acatctctgt
     6301 gtttgcgagg gtgtgggcgt gttcgatgtt tcccatatgg gagaattcgc cgtaagcggc
     6361 cccagggcgc tggacttcct tcagcaaatt tgctccaacg acgtgtcggt attgaccgac
     6421 ggccaggctc agtacaatta tttccccaat gagcaaggcg gcatagtgga tgacttgttg
     6481 gtgtatcgca taaacaagaa cgaatacctg ctggtggtga atgcctccaa catcgaaaag
     6541 gactgggatt ggtgttgttc gcacaatcag agcggcgcca aactcgaaaa cctctccaac
     6601 cgcactgccc agctggccat ccaaggccca aaagcattgg atgtgttgca aaaattaagt
     6661 ccggtcgatc tcaaagccat tccttattat cattttactt acggcaagat ggccggtata
     6721 gatcaggtct tgatatccaa tacaggttat acgggcgccg gtggttttga actctatttc
     6781 aagcccgaag acggattgaa actctgggaa gccatttttg aagccggagc cgaatacggc
     6841 attcagccca ttggtctggg cgcccgcgat accctgaggc tcgaaatggg tttttgcctg
     6901 tacggcaacg aaatagacga taccaccagt ccgttcgaag ccggcttggg ttggattacc
     6961 aaatttttgc ccggcaaaaa cttcccgggt agggcgattt tggaaaaaca aaagcaagaa
     7021 ggagtaaatc aaaaactctg cggcctggaa atgatcgata aggggattgc taggcaaggc
     7081 tatgaagtgg ttgatgctca gggtgaagct ataggacggg tgacctcggg taccatatcg
     7141 cccatgctca aaaaaggcat agcgctggcc tatgtgaaaa cgccctttac acagcccggg
     7201 acggaggttt ttgtgcgcgt tcgcaatcgc gacctcaaag ccaaagtggt taagcttcct
     7261 ttcagaaaat aaaatccggc gccgatgctt gcctgcccgg gttaaagaga gaaaatcaag
     7321 tttcgcttac taactcagcg ttcagtcggg ggggggatag gatcaattca atgaaggaat
     7381 gagcaatcga cggcaagccg agccataaca acgttgtcgt ggataagatc aattcagtga
     7441 aggaatgagg ggatacatgc tccagacctt gtatttttcg cccaattgcc gcaagcattt
     7501 tttccacagc gtggagctcc tcgattggaa gatttctttg aggccgattt tgcccaccag
     7561 ccaggaatcg tctctgattt cggcttcgag ctgcccttcg acccagccgg aataacccac
     7621 aaagaaacga attttgtttt tggcttcttc gtccagggcc aacaggctca gcaattgatt
     7681 taaatctccg tccatataca actcgtcgct gaccgcgatc gatcccttga tttcgctgcc
     7741 cagcgagtga acgtaaaaca agcgtttggc tgccaccggg cctcccaggt aaataggaat
     7801 tttggggatt ctctcgttgg tgctgaccac ttcgtccaga ttgaatccta taggtttgtt
     7861 cagtacaaag cccaagtgtc cgttcggccc actttcgcac atcagcacca cggaacgttc
     7921 aaaaggttct ccggccagaa agggttccgc tatcaggatg cgtccttttt cgggcttcag
     7981 tgcgttgtgt ttgatgataa ggtcctggat acgcataact tgaatctttt tcttagttca
     8041 gcggatggtc tgcccggaat tttttaagcc gggattccaa ttcttcgtat tgatcgtctt
     8101 tgataaacga ggtacaaatc ctgaggcctt gctggtcact gccggtggtt tcgagcgaga
     8161 tggcactgat gccgtaaaaa agcaattctt tcatcaactc cgctccgctc atgcccgggt
     8221 aacgtaccgt aaaataaaac ccgtcggcca gggcttcgcc caagtcgttg tcgtaaacaa
     8281 tctcgaattg atttccgagc atgagtttct ttactttttc ggcgcgccgg ccgtattccc
     8341 tcacttcgtc caggaagcga tattcaccgt cggaagccgc cttcatcatg gcagccatag
     8401 cgtattgggc cgaatgactg gtgcccgacg acagcgaata gagtacccgg ttgacgaaaa
     8461 cgttgccgaa ttcgcacacg ccgtattgct ttttgaggtc tgcgtaaggg cgcttataca
     8521 gtttttcgga gaggacggct acgcctatgc gttgtccggc gtagctgaag gctttggaag
     8581 cggagatcag caatatgtaa ttgtcggtgt attttgctac gctgggctga aagggcggtt
     8641 caaaaggtcg ccccaaatcc tggcggaagt ccatggcaaa ataggccagg tcttcgataa
     8701 cgatgacatc gtatttttcg gccaattccc caattatttt caattcgctg tctttcaggc
     8761 agaaccaggc cgggttattg gggttggagt agatcagggc cgaggtttta cccaggctca
     8821 ggatgctttc gagtttttcc ctcagagctg acccccggta gtggtacacg tcgaacgaat
     8881 cgtaggcggt gttgagcacc accagttgtt gcttttgtac cggaaagccg gggtcgataa
     8941 agagcaggcg gtctttgctg cgattgcact gactgcaggt gataaaggag gtaaagctgc
     9001 cctgcatgga gccggtgacg ggcacacagc attcggcggg cacatccaca tctataaagg
     9061 ccttaacaaa acgggcggct tccgctttga gctccggatg tccgtcgagc atggggtaga
     9121 tggaggccac gcctttttgc aaggctgcta tttcggcttc gacccctact ttggaggggg
     9181 gaaggccggg cacgcccatt tccatacgga cgtagcgttt gccgctgcgt ttttcggcct
     9241 ggttcaccat agaaacgacc tctcgtatgg tggctttgcc gaaatcgggt aaattatatt
     9301 cgtccagaat attctgtact atgacagaat cgatgggggt atctcgctgt tgactcattt
     9361 gccttaatgc tttaatgctt taatgccggg gttgtaggtg tctatcgttc tttccctgat
     9421 cgggatggac aaaggtatag caattagccc ggaaataaaa atgcaaaaaa tcccatcctt
     9481 tgcttgcaga ttgattttat ctgcctatct ttgtgcccgc aaatgatgat ttgcttaccc
     9541 ggtgtggtag ttcagctggt tagaatacct gcctgtcacg cagggggtcg cgggttcgag
     9601 tcccgtccat accgcaaaaa gcgacttgaa aaagtcgctt ttttttgttt tcggaactcg
     9661 ctccgcacaa ctcgtgtatt ctgcccgata cgcttgaaga cggattgaga tgcaaatcgg
     9721 attcttgaat atagatattt gtatctgatt ttccatagcc cagacatatc cttaatgcat
     9781 caatgattgt tgttttacct gagttgtttt ctccaattat gatgtttact cctttgttaa
     9841 acagtagaga tatttcatcg aacaccctga attttttata ttaaattgtt ttatatacat
     9901 actgtactga ttgtaaagtg aatttcaaaa caactccctt ggattaatct ttaaaaactc
     9961 ctcatacggg attccacccg tgcccacaag taacactttt tccggatgaa acttctctgc
    10021 aaatactccc atcccggtat tttccgcctt cattccgctt ttgacttcaa gcccgattac
    10081 tttgtctcct ttttccaata taaaatccac ttcatggttg ccttcgcgcc aataatataa
    10141 attgtatctc tccggaatag aattatttat caaatgtgtt ccaacagatg attcgacgag
    10201 tcgcccccat aattcagggt taataatcgc tttctcgtag gcgtcattgg cttgagaagt
    10261 aattaacgca ttattataca cttgaaactt tggacttgaa ccgcgtttac ggataatatc
    10321 tccggcgtat ttatccagcc cccctaataa tccacaatca gacagtaatt tcaggtaatt
    10381 cgctaatgtc gtcgtattgc ctgcatcttg aagttgacct attatttttg tataagaaag
    10441 aacttgtccg gaatatagag aaccaagttc aaacaatcgt ttcagtaaag cgggcttatt
    10501 tacccttgtc agcattaaaa tatcttttga aatactggtt tcaatcagcg aatctttaat
    10561 ataatttttc cagcgctctt cgtcgtcaat taaagtggct gaccccggat atcccccaaa
    10621 gtacacatat tgttcaatac tccatccaaa cgcttcctgc atctcggtat aagtccagtg
    10681 acctaaatat attgtttcaa atctaccggc caaagattca gttaaccctt gttgaattaa
    10741 taagcgtgat gagcccaata aaattacttt tatgtttgtt ctctctcgcg tatccttatc
    10801 ccattgttgt tttacaatct cgctccagtt atctattttc tggatttcgt caataacaat
    10861 taaatactct gttgcaccgg atactttaat ttgtaatctg gctgcctccc acagttgtgt
    10921 cagccataca gaattggtga cgggaatagc gtctgccgat tcatataaat tgggaaccga
    10981 cagttgggac aagagctggg ttatcatggt agtttttcca acttggcggg ggcctaagat
    11041 aacttggata aatttccgag gctcttgaat tctcttctta accgtctcta aataggatct
    11101 gtaaaacata cttttttatt ttactcaata taggtagcaa atttactcaa tactttgcca
    11161 tattcaaata tttttccatg aacttttcac gattatcgaa agatatttgg gaatggaaac
    11221 tattgcattg agttaggtgt atcggggaag tatgtcggta attttgctca tattatcggc
    11281 tattcagcaa atcttctaat ttccacgcgg atatatgttc tatcccttca ttggttggta
    11341 tgttgtaatc gtccatactt accacatact tatcgaagtt atcatcgatg agttttaacg
    11401 aacggtattc tctctctatc gtgtgttctt cggtaaggct gagagttact tgcaaataga
    11461 gccttctgtc gccttttatt gccacaaaat cgacttccgt gttttttatt gttcccacat
    11521 aaatttggta tcctgcacgt cgcaaactca aataaacggc gttctccaaa agataaccaa
    11581 ttccgtaacc aaagcctgta taaagataat ttcggtagct taaatcgttc atatagtatt
    11641 tgctgttgcc tgaaatggtg tctttgcctt ttatgttgta tcgctccaca cgatgaacaa
    11701 gaaatgagct ttccaaatag ctaatgtacg atgaaagcgt ttcgtagttt gtttttcgct
    11761 ttttcgatgc aaaataatgg atgatattag ttatcgagat caggttagag gcactgttta
    11821 ccaaataaac aaacaaatca tccaacaact ttacatcttt tacattatac cttgctacaa
    11881 tatcacgcaa cataaccgtg tctttgatgg acgacacgta gttttgtttc atttcatcgt
    11941 taggcaaatt aaatagttcc ggcaaagctc cgtcttgaag gaattcttta taactttcac
    12001 tgtttttttc ttgttgagta actccgcaaa attcagtata actgtatgga agtacttcaa
    12061 actcaacata tctgcctgac aacatagtgg ctaattctcc ggaaagtaac ttggaatttg
    12121 agccactgat aaaaagttca caaggttctg caaagtcctg agaatgtgag tttacaaagc
    12181 gttcccattc ttcaatatat tgtatttcgt caatgaaaat atagacttta ccgctagggt
    12241 ttagtacttc tctataagct ttgtacaatt gctctaactc tatggcactt cgtaaagtgg
    12301 acagctcgat atattctttg tttatgtaaa gaatattttc actttttaca cctccggaca
    12361 ataacttgga tgcaatttgg cgtaagatgt agcttttgcc cgcacgacgt tgtccgacaa
    12421 gcacttttac caacttattg cccacatatt gtccgatttt atctgtataa aatgtacgag
    12481 gataacctaa atctatggga ttattatccc aaaaattgta ctttcgtata gaattaatta
    12541 tttcaaccat acttgaatat tttctgcaaa tataattatt ttcaaatgta tttgaaaatg
    12601 ttgtcgattt ttttattctg tttattttcc caccaattat taaatttgct catcgtacag
    12661 gcttttatat cgtcggcaat atagggtttc atcgccttat aattacgtgc caggctttgt
    12721 aattcttttt ctgctgttct ttgtatcaac atgagtttcc attgtatagt taggggatgc
    12781 agatatacaa ttccggaatc gaattccgga attgcaaagt attatgtttt ttactttctg
    12841 cccttattct tcatggaaag acagagcaat gcttcagcac gaaaaaccgc tattgaccgc
    12901 cctttatctc aataaaatcg ggcacaaaca gtacaaaagt ttctgtttgt caatcgagct
    12961 acaacgatta aaatttcgat tgtccttact gcaggcatat gtaaacagaa atgaaagacc
    13021 cgacttgaaa gccgtcgata aaaaagtaga aacagcattt gaagagtaca atcgacaaat
    13081 agaagctgaa gctgaaaggc ttgccgccgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn catttgaaga gtacaatcga caaatagaag ctgaagctga aaggcttgcc
    13261 gccgccaaca ggtttttgtc gggaaatttt ttgacgagcg aaaacagtaa aaagctgaga
    13321 gaactctact atttcattgt aaaacgtctg catccggata tcaacgccga tctaagcgac
    13381 aatatgaaag atttgttcat ttaggcgcag gctgcttacg accttggcga attggaaatc
    13441 ctgcagcaga tagcgttatt attgaagcac gacagtgccg aaacagccaa tcttacaccc
    13501 gaataactcg ccataggcat atatcacaac ggcattcgta tcggatgggt acctcaggag
    13561 attaatgaaa tgatatcgag attgatggat gccgggaaag ccttcttttg ccgaatcaca
    13621 tcagcaaaac ggaaaaataa atggctgaag gtttataaaa ttttaaaaaa caatacttct
    13681 tttcaaaatg tttttgcatc tttgaaaaac cttgtatcga caagttgatt tttcaaatca
    13741 atacttatgg gaaacaccaa aatcgtaacg atacgtttca aaaatattat atcgccaaca
    13801 gatatagagg cttttcgagg agctgtagtc aatgctctca aagacaagga tatcctattc
    13861 cacaaccaca atcttaccga taaaggtttt cgctattctt acccgctcat tcagtacaaa
    13921 agaatcaata gaagggcagc cattttttgc ctggaagaag gtaccgaatc tatcggcaag
    13981 ttctttctag attcagattt gacccttaat ctcaatggga aaatacatca attcgaagta
    14041 gaaagcgtca aagcccacaa gcatcttatt caaatctgga attccaatat tagatacacc
    14101 ataagaaaat ggttggcact caatcaggag aattacgagc ttttcgacac attggaaagc
    14161 gtggctgaaa aatccgcttt tttagaaaac atccttaagg ccaatatatt atcctttgcc
    14221 aaagggctga atgtcttttt tgaaaaacag gtagaatgta aaatcacccg gcttagcgaa
    14281 cctcgtatta cactatataa aaacgtgaaa atgaccacgt tcgatgccga atttctcacc
    14341 aatgtttcca taccggacta cgcggggctg ggaaaagggg tgagtgtcgg gtatggggta
    14401 acggtgagga agaaagagaa a
//
LOCUS       DAOSTD010000041        14400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_4275_length_14400_cov_15.355037, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000041 DAOSTD010000000
VERSION     DAOSTD010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14400
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4275_length_14400_cov_15.355037"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..779)
                     /locus_tag="PLI37_04225"
     CDS             complement(213..779)
                     /locus_tag="PLI37_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58254.1"
                     /translation="MKTSILKVLGLGIIAGMVFYSCGSSKKITPKKDGEVEIVSYCSG
                     SEYQSNNKAFRFTGIGESMNQMTAKNMAMSQARAGLAATINTTIKTVTDNYVKSGNFN
                     NREELLNNYEGMTREVVNQTLSGAVVICEKMTRTQQGNYKAYICLEYGANDILQNINN
                     RATSQEILKVDYNYEKFKSTFEEEMSKF"
     gene            complement(819..3446)
                     /locus_tag="PLI37_04230"
     CDS             complement(819..3446)
                     /locus_tag="PLI37_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58255.1"
                     /translation="MKTPILSIILFVCLGLQAQSRSFEEYMRREQEKMKQFEQEQIEG
                     MERLRKEYAEYVAKRDQEWADYLKKEWENYSAFAAKELPAKPKPKTNPVFDMSSQTES
                     KTISAARAESLKPEPLPAVVEQVRKPAEKAVNFRTANLNFYNRSLAIPYDPAMRAFVL
                     QGISQESIGAFWEKISESNYTPAVESLLEAKKELNINDYAYMLLCQYFSKSLYPANAN
                     AARLMTWFVMLRSGYSLRVGFNDEAIALLVPTQDQVYQKAYLNIDGINYYIFPDLKNN
                     SLYTYDEDYRSGKPVSFGIHSPMNLGGKTAERKVKFEFEGKTYDIKLNYDPDLIDFFQ
                     YYPLLSFETYFNAPMSVQSRETLYAALQPVIGEMDEVRAVNFILRFVQTAFAYKTDPE
                     QFGREKYFFAEEVLHYPYCDCEDRSVLFSYLVRELLGLNVVALEYPGHMATAVAFKSP
                     FSGNHLVYNNQRYVVADPTFINAPVGVAMPEYSNATPIIHEIANPQGRAFSLSGLWDD
                     FSEKGCYKGSFRKNAKLLADGSALLTGYFSKAVQLGSFNLPGKGEAHNCFVAKLNPEG
                     KVLWANKLSSSGNAVGMSVDCQASGDIVVAGVFTGELQTGGYKLLAPEGKSDLFMAGY
                     SPEGRLLWLSKAGLDELPQTIHTAFTAVFSSDGINLSTQHAGEQLEESQQGLFIAPDG
                     QILYSGMVNNALAVAGNQQPVAFASEATLDIPALFKAESAKFVNQKTDPAMAGLFAAC
                     KMVKDMGISLTGKSVQETLDKNNPGFKTSCPNIYKNFGSIDFVRNAQGIITILTVGKK
                     NISFDKVRISNNSTISITEIPGNNYRVDALSGIKVGMAVVWFDLNFIKLMSSNGDMIF
                     DYASDHSQVSVNLQKDILD"
     gene            complement(3714..6761)
                     /locus_tag="PLI37_04235"
     CDS             complement(3714..6761)
                     /locus_tag="PLI37_04235"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOQ58256.1"
                     /translation="MRIPKLIFGFCALFTTALTAQNGDQILDGIGETGLIARYTFKSN
                     ADDWSRNNLHGSIKGDDYRFAADSLFGTVLSLPEEAESYVIIPGESVSGEESLSISAW
                     IYPRSTGEKQVFFDFGKNSQSRLLVTLTGGGEKTAILAQVQTPAKTYELSSEALAINS
                     WHHLALVINLPSKSLNMFINGKIAKEVQKVELSLADLFDMETGKENKLLIGKSQSPGN
                     KPLNAALHDFRLYRIPLNEWQIARIYGIALNKEEPIVRRRRTEANRELPVFPPTTPQL
                     YNAFLTAVPDIEVETVTGQLPRLPRYLKGVYEKGIEGPDVRVIWPSPTDNSQVLTPGT
                     YEVTGKVSGTDLQPKALVTVKTAEKQNGPQRKVEAFQLDRVTLNTDCHGRNTKFIENR
                     DKFFNGLGATNPDNFLYMFRNAFGQQQPEGAEPLGVWDSQQTKLRGHATGHYLTALAQ
                     AYAGSAYDKDLQAQFAQKMDYMVDVIYELAQLSGTPQSPGGACVTDPCQVPPAEGKAS
                     YDSDLSEKGIRTDYWNWGKGFISAYPPDQFILLEQGATYGGSNSQVWAPYYTLHKILV
                     GLMDIYEICGNKKALDVVTGMGDWVHARLSNLPKETLINMWSRYIAGEFGGMNEAMAR
                     LSRLTGEKSYLETARLFDNINMFYGNPEHSHGLAKNVNSFRGFHANQHIPQIVGALEI
                     YRDSQAPEYFCIADNFWTMVTGDFMYSIGGVAGARNPNNAECFTAQPASLYENGFAAG
                     GQNETCATYNMLKLTSGLFMFDQRAELMDYYERGLYNHILASVAEHSPANTYHVPLRP
                     GSVKQFGNPHMNGFTCCNGTAIESSTKLQNSIYFKSTDNKTLYVNLYVASTLQWKERK
                     ITITQSTAYPNEDQTKLTVQGKGKFELKVRVPHWAKKGFFVKINGKEIEVDAQPGTYL
                     SLNRKWKKGDVVELRMPFSFYLEPVMDQQNIASLFYGPVLLAAQEEEARTDWRKITLD
                     AKDIGKSITGDPSSLEFKIGDVLFKPFYNTYGRHSVYLDVSLK"
     gene            complement(6952..8076)
                     /locus_tag="PLI37_04240"
     CDS             complement(6952..8076)
                     /locus_tag="PLI37_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOQ58257.1"
                     /translation="MQRTFLLLTTVVLLFCSHLTAQTTLRQATEDLFLIGVAVNNPQT
                     RGARPAEAEQISRHFSAIVPENCMKSAEIHPEEHRYNFEATDQMLAFGENNRQVVTGH
                     CLIWHSQLARWFCVDEQGEPVSAEVLKSRMREHIFSIMTRYKGRIKGYDVVNEAFEDN
                     GTYRNSPFYRILGKDFIRLAFEYAHQADPEAELYYNDYNMANPVKCDAVVKMVEELKA
                     AGCRIDAVGMQGHVHMDEPSVEAFETSILKLAATGVKVLITEWDISILPNPYRHTGAN
                     IADRFVYSTQNDPYREGVPDEVMKAWEQRVTELFALFLKHHRHIDRVTLWGLNDGNSW
                     RNNFPVRGRKDYPLLFDRDNQPKAVVQQMIELALKAKTKQ"
     gene            complement(8159..10162)
                     /locus_tag="PLI37_04245"
     CDS             complement(8159..10162)
                     /locus_tag="PLI37_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase"
                     /protein_id="HOQ58258.1"
                     /translation="MPSGKSSPTLELAKQVLIDGWQDRENNSIKLKISRDKRLSEQGY
                     SLSPGCITAKTHQGLLYGAYEYLRRRQIGLSLELPFSNPSYQRRLLNHWDNLDGSIER
                     GYAGRSIFWKGKQSMEPTAEDRERWRTYAAINASIGINGAVLNNVNAPPEALSLPVLK
                     RAAAIADELRPYGMVSYLSINFSTPMRLGGLKTADPLDPEVVDWWRAKIREIYSLIPD
                     FGGFLVKASSEGMPGPGDFGRSHAQGANMLAELLKPYHGILMWRAFVYKPSDNDRAKQ
                     AYEEFMPLDGQFCDNVVVQIKNGPIDFQPREPFSPLFGALRKTAVLPELQITQEYLGQ
                     EHQLAYLGGLWEEFLQSDTYQSGPGSTVARCTDGSLFDQSLTAIAGVSNIGSDSNWCG
                     HPFAGANWYAFGRLAWNNRASAAGIAEEWLRLTFEPTQSPKSNSLSDESPALDNDPAS
                     DQKNGNWEKEFLQPVLSMMLRSREAMVNYMMPLGLHHLFALDHHYGPGPWYDSPRQRK
                     DWTPPYYHQADAKGIGFDRSSGGSNAVSQYREPLRSQFDNPGSCPENLLLWFHHLPWE
                     HRLRNGNSLWEELCRRYDAGLQEVRRFQLVWDNMENRVDSEVFTQVQAKLRRQARDAQ
                     VWKDACLLYFQSINQLPFPEDMERPVHDLEVLKKTDMRYFMNR"
     gene            complement(10497..10569)
                     /locus_tag="PLI37_04250"
     tRNA            complement(10497..10569)
                     /locus_tag="PLI37_04250"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10534..10536),aa:Phe,seq:gaa)
     gene            10707..10782
                     /locus_tag="PLI37_04255"
     tRNA            10707..10782
                     /locus_tag="PLI37_04255"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10740..10742,aa:Pro,seq:cgg)
     gene            10846..>11987
                     /gene="serS"
                     /locus_tag="PLI37_04260"
     CDS             10846..>11987
                     /gene="serS"
                     /locus_tag="PLI37_04260"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOQ58259.1"
                     /translation="MLTLKYLSENTRECIERLAVKHFDAEALIGEILELDLQRRNAQT
                     RSDACAAELNTLSRSIGVLMQEGKKQEAAGVREKTLQLKEEGKRLEIARKEAENRIQE
                     LLVQIPNLPHSSVPAGKGAEDNVTERSGGPMPDLPVDALPHWDLAKKYDLIDFELGAK
                     ITGAGFPVYKGKGARLQRALINYFLDRARQAGYLEIQPPYVVNAASGYGTGQLPDKEG
                     QMYHVGLDDLYLIPTAEVPVTNIYRDVILDEKDLPVKNTAYSACFRREAGAYGKDVRG
                     LNRLHQFDKVEIVCIEHPDRSYERLEKMVAHVQSLVEALELPWRILRLCGGDMGFTSA
                     LTFDFEVYSAAQQRWLEVSSVSNFESFQANRLKCRYRTAEGGKIQL"
     assembly_gap    11988..12087
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12088..13613)
                     /gene="pckA"
                     /locus_tag="PLI37_04265"
     CDS             complement(<12088..13613)
                     /gene="pckA"
                     /locus_tag="PLI37_04265"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOQ58260.1"
                     /translation="MKSLDLTKYGIKGTTEIVHNPSYEQLFKDETDPNLQGFERGQVT
                     EMGAVNVMTGVYTGRSPKDKFIVMDDVSRDTIWWTSDEYKNDNKPLPKASWGVLKDIV
                     TKQLSNKKLYVVDTFCGANEASRLKVRFIMEVAWQAHFVKNMFIRPTEEELKNYGEPD
                     FVSLNGSKTCAGAQYKELGLNSETFTVFNLTEKMQVIGGTWYGGEMKKGIFTLMNYYL
                     PLQGIASMHCSANVGKDGDTAIFFGLSGTGKTTLSTDPKRQLIGDDEHGWDDEGIFNF
                     EGGCYAKTINLSKEQEPDIWKAIRRDALLENVTVDANGKIDFNDGSVTENTRVSYPIY
                     HIDNIVKPVSKAGPAKKVIFLSADAFGVLPPVSILTPEQAQYYFLSGFTAKLAGTERG
                     ITEPTPTFSACFGAAFLALHPTKYAQELVKRMQASGATAYLVNTGWNGTGKRITIKDT
                     RGIIDAILDGSIDKAATKEIAYFNFKVPTALPNVNPAILDPKDTYADGKVWEEKAKDL
                     A"
     gene            complement(13918..>14400)
                     /locus_tag="PLI37_04270"
     CDS             complement(13918..>14400)
                     /locus_tag="PLI37_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOQ58261.1"
                     /translation="IQSVEFLCSLPYVDTERIAALGFSMGAHRAWMLAAASDKVSAAV
                     AVCWMNTTEHLMTLDNNQNKGGSAYSMLLPEIRNYLDYPHVASIACPKPMYFINGSRD
                     KLFPVQGCLDAFETMRRVWESQGAGDCLRTEIWDSPHFFSRAMHEQSLQFLNKFLRKH
                     "
BASE COUNT         3400 a         3266 c         3682 g         3952 t
ORIGIN      
        1 agctctccct ggtcttggct tcgggccggt aacaggcata ctgcgagcag cagcaaaaat
       61 aaaaatctgt atctggtttt cataataggg ggttcttgaa cgatgtcatc ggatacctgt
      121 ttgacataca gcccagttga cgggcgctaa acagcccgtc aacttagtat tttctctgtg
      181 ttcaatcttt tataggctac aaaaaggaaa attcaaaatt tgctcatttc ttcttcgaag
      241 gtgctcttga atttttcgta gttgtagtct actttaagaa tttcctggct ggtagcgcgg
      301 ttgttgatat tttgcaagat gtcgttggct ccgtattcca ggcaaatata ggctttgtag
      361 ttgccttgct gtgttctggt catcttttca caaatcacaa ctgctccgct cagggtttgg
      421 ttgaccactt cgcgggtcat cccttcgtag ttgttcagta attcttctct gttgttgaaa
      481 tttcccgatt tcacataatt gtcggtaaca gttttgatgg tagtgttgat ggtggcggcc
      541 aatccggcac gagcctgact catagccata tttttggcag tcatctgatt catgctttca
      601 ccaatgcccg taaaacggaa ggctttgttg ttgctttggt attccgaacc ggagcaataa
      661 gaaacaattt cgacttcgcc atcctttttg ggtgtgattt ttttggaaga accgcaggaa
      721 taaaacacca tgccggcgat aatgccgagt cctaatactt tcaagataga tgttttcata
      781 atttttgaat ttaatgggtt gataaaaaat gattgcaact agtctaagat gtctttttgt
      841 aaattgaccg aaacctgtga gtggtcggat gcatagtcaa aaatcatatc cccgttggag
      901 gacattagtt ttataaaatt caaatcgaac catactacag ccattcctac tttgatgccc
      961 gaaagcgcat caacacggta attgttgccc gggatctcag taatggaaat ggtactgttg
     1021 ttggagatcc tgactttgtc gaaggaaatg ttctttttac ccacagtcag gatggttata
     1081 atgccctgag cgttgcggac gaaatcgatg gatccgaaat ttttataaat attggggcaa
     1141 gatgttttga aacccgggtt gtttttgtcg agagtttctt gtacagactt gccggtgagg
     1201 ctgatgccca tgtcttttac cattttgcag gcggcgaaca gaccggccat ggcgggatcg
     1261 gttttttgat ttacaaattt ggccgattca gctttgaaca aagcgggaat atcaagtgta
     1321 gcctccgatg cgaaggccac cggctgctga ttgcctgcca ctgccagggc gttgttgacc
     1381 atgcccgaat acagaatttg tccgtcggga gcaataaaca ggccctgctg cgattcttcg
     1441 agttgttctc cggcgtgttg ggtggacaaa ttgataccgt cggacgagaa taccgccgta
     1501 aatgccgtat gaatggtttg aggcagttcg tcgagccctg ctttactcag ccagagcaga
     1561 cgcccttcgg gggaataacc ggccatgaag aggtctgatt tgccttcggg tgcaagtaat
     1621 ttgtagcctc cggtctgtaa ttctccggta aatacaccgg ctaccacaat atcgcccgaa
     1681 gcctggcagt cgaccgacat tccaacagcg ttgcccgagg aactcagttt gttggcccat
     1741 aatactttac cttcggggtt gagtttggct acaaagcaat tatgtgcttc gcccttgccc
     1801 ggtaaattaa agcttcccaa ttgtactgct ttggaaaaat agccggtcag cagggcggat
     1861 ccatcggcca ggagtttggc attctttcgg aaactgcctt tgtagcaacc tttttctgaa
     1921 aaatcatccc aaagtcccga tagagaaaag gctctaccct gagggttggc tatttcatga
     1981 ataatgggtg tcgcattgct gtattcgggc atagccacgc ctacaggtgc attgataaaa
     2041 gtgggatctg ccacaacata acgctggttg ttataaacga gatggttacc gctaaatgga
     2101 gatttgaagg ctacggcggt tgccatatgc ccgggatatt ccagggcgac tacgttgagc
     2161 cccagcaatt cgcgcaccag gtaagaaaac aagaccgagc ggtcttcgca atcgcaatag
     2221 ggatagtgta agacttcttc ggcaaagaaa tacttttcgc ggccgaattg ctcggggtcg
     2281 gttttatagg caaaagccgt ctgaacaaag cgcaggataa aattaaccgc ccggacttcg
     2341 tccatttcgc cgatcacggg ctgcagggct gcgtacagag tttccctgga ttgcacagac
     2401 atgggggcgt tgaaataggt ttcaaaagag agcaggggat aatattggaa aaagtcgatc
     2461 agatccgggt cgtagttgag cttaatgtcg taggttttac cttcaaattc aaatttaacc
     2521 ttcctttcgg ccgtttttcc acccaggttc ataggcgaat gaatgccaaa gctcaccggt
     2581 ttgccgctgc ggtaatcttc gtcgtaggtg tacagcgaat tgttcttcag gtcggggaaa
     2641 atataataat tgatcccgtc aatgtttaaa taggcttttt gatagacttg gtcctgagtg
     2701 ggaacaagca gggcaatggc ctcatcgttg aaccccacgc gcagggaata gcccgatcgc
     2761 agcatcacaa accaagtcat cagcctggcc gcattggcgt tggccggata gaggcttttg
     2821 gaaaaatact ggcaaagcag catgtaggca taatcgttga tgttgagctc cttttttgcc
     2881 tcgagcagac tctcgacggc cggagtataa ttgctttcgg aaattttctc ccagaatgcg
     2941 cctatacttt cctggctgat gccttgcaac acaaaagctc tcattgccgg atcgtaggga
     3001 atggccaggc tgcggttgta gaaattcagg ttggcggttc tgaaattgac ggctttctcg
     3061 gccggtttgc gaacctgttc caccacggca ggcaggggtt ccggttttag actttcggca
     3121 cgggcagcgg aaatagtctt gctttcagtt tggctgctca tatcaaacac cgggttggtt
     3181 ttgggtttgg gtttggcggg taattctttg gcagcaaagg ccgagtagtt ttcccattct
     3241 tttttcaggt aatcggccca ttcctggtca cgtttggcca catattccgc gtattccttg
     3301 cgcaggcgtt ccattccctc gatttgctct tgttcaaatt gtttcatctt ttcttgttcc
     3361 cggcgcatgt attcctcgaa acttcgggac tgtgcttgca aacccaggca gacaaacagt
     3421 attatgctta gaataggtgt tttcatgttc gtatggtgtt aaatttgaca ggatttctta
     3481 aacaatcttt ttgataatta caacagaaat aacaatcaga ctacaatgat acaaaaaaat
     3541 gcgaatagcc ctatgttata tccaaaaaaa aaacaattcg attgtttttt atagaggtat
     3601 gttgcgaatt cgccatccat aaagccaaaa gcaagttccg aactttttcg gaacttgctt
     3661 ttgagtaaaa tattaagggt taggactgaa agggtctatt ttaagcttaa aggctatttt
     3721 aggctgacat ccagataaac ggaatgacgt ccgtaagtgt tgtaaaatgg tttgaacaaa
     3781 acatcgccga tcttgaattc caggctggaa ggatcgcctg ttatcgattt accgatgtct
     3841 tttgcgtcga gagtgatctt gcgccagtcg gtgcgggctt cttcttcctg ggcggccagc
     3901 agaacaggcc cgtaaaacaa gctggcaatg ttttgctgat ccattaccgg ttccagataa
     3961 aagctaaaag gcatacgcaa ctccacgaca tctccttttt tccatttacg attcaggctc
     4021 aggtaagtac ccggctgagc gtcgacctcg atttctttac cgttgatttt tacaaagaag
     4081 cctttttttg cccaatgcgg cactcttacc ttcaattcga atttgccttt gccctgtacg
     4141 gtcaatttag tttggtcttc gttgggataa gcagtacttt gagtgatggt gattttacgc
     4201 tccttccatt gcagggttga tgctacgtag agatttacat acagggtttt gttgtcggta
     4261 cttttgaaat atatggagtt ttgcaacttg gtgctgctct cgatcgcggt accgttgcaa
     4321 caggtaaatc cgttcatgtg cgggttgcca aattgcttca cggagccggg tctcaaaggc
     4381 acgtgatagg tattggccgg actatgctcg gccaccgaag ccaaaatatg gttataaagc
     4441 ccgcgttcgt agtaatccat caattctgcc cgttgatcga acataaacag accactcgta
     4501 agtttgagca tattataggt ggcacaggtt tcgttctgac cgccggcagc aaaaccgttt
     4561 tcgtagagtg aagccggctg ggcggtaaaa cattctgcgt tgttgggatt acgggctccg
     4621 gctacgcctc cgatgctata cataaaatcg ccggtgacca tagtccagaa attatcggca
     4681 atacagaagt attcgggagc ttgcgaatcg cggtagattt ccagtgctcc cacaatttgg
     4741 ggaatgtgct ggttggcatg aaaacccctg aagctgttta catttttggc cagtccgtgc
     4801 gaatgttccg ggttaccgta aaacatattt atattatcaa acaggcgggc ggtttccaaa
     4861 tacgattttt cgcccgtgag gcgtgacaac ctggccatgg cttcgttcat acctccgaat
     4921 tcgccggcta tgtaccggct ccacatattg ataagggttt ctttgggtaa gttactcaaa
     4981 cgggcatgta cccagtcgcc catgcctgtc acaacatcga gggctttttt gtttccgcag
     5041 atttcataaa tatccatcag accgaccaga attttatgca gggtgtagta aggtgcccac
     5101 acctgagaat tggaacctcc gtaggtggct ccctgctcaa gaaggataaa ctgatcgggc
     5161 gggtaggcgc tgataaagcc tttgccccag ttccaataat cggtgcgtat gcctttttcg
     5221 cttaaatcgg agtcatagct tgcctttcct tcggcgggag gcacctggca gggatccgtg
     5281 acacaggctc ctccggggct ttgaggagtc cccgacagtt gggccagctc ataaatgaca
     5341 tcgaccatgt agtccatttt ttgagcaaac tgagcttgta ggtctttatc gtaagcactg
     5401 cctgcatagg cctgagccag tgctgtaagg taatgccccg tggcgtgtcc gcgcagtttg
     5461 gtttgctggc tgtcccatac acccaaaggt tcggcgccct caggttgttg ttgcccaaag
     5521 gcgttgcgga acatgtagag aaaattatcg ggattggtag cgcccaggcc attgaaaaat
     5581 ttatcgcgat tctcgatgaa tttggtatta cgaccgtgac aatccgtatt cagcgttacc
     5641 cggtccaatt gaaaggcttc tactttgcgt tgcgggccat tttgtttttc ggccgtcttt
     5701 acggtaacca gggctttggg ctgaagatcg gtgcccgaaa ctttgccggt cacttcgtat
     5761 gttccggggg taagtacctg tgaattatcg gttggtgagg gccagatcac tctgacatcg
     5821 ggtccttcta tacccttttc gtaaactcct ttcaaataac gtggcaggcg aggcaactgt
     5881 ccggtcactg tttctacttc gatgtcgggt acagcagtta agaaggcatt atacaactga
     5941 ggagtggtgg ggggaaatac gggcagttcc cggttggctt ccgttctcct tcttctgact
     6001 atcggttctt ctttgttcag ggcaatgccg tatatcctgg ctatctgcca ctcgttcagc
     6061 ggaatacggt aaagacgaaa atcatgcaaa gctgcattca agggtttgtt gcccggagat
     6121 tgcgattttc cgatcaggag tttgttttct tttcccgttt ccatgtcgaa caaatccgcc
     6181 agactcagtt ctactttttg tacctctttg gctattttgc cattgataaa catattcagg
     6241 ctttttgacg gcagatttat gaccagggcc aggtggtgcc agctgtttat agccagggct
     6301 tcagagctca attcataggt tttggcagga gtctgcacct gagccagaat ggctgttttt
     6361 tccccaccgc ctgtcagagt taccagaagg cgtgattgac tgttcttgcc gaaatcaaaa
     6421 aacacctgtt tctccccggt cgaacgcgga taaatccagg cgctgatact gagcgattct
     6481 tcgcccgata cagattctcc gggtataatt acatagcttt cagcctcttc cggcaaggat
     6541 aataccgtgc caaaaaggga atctgccgca aaacggtagt cgtctccttt gatactacca
     6601 tgtaaattgt tgcgcgacca gtcgtcggca ttgcttttaa atgtataccg ggcaattagg
     6661 ccggtttctc ctataccgtc caaaatctga tcgccgtttt gggccgttaa ggcggtggtg
     6721 aataaggcac aaaagccaaa aataagtttg gggattctca tggttgttat tgagtatgaa
     6781 gaaatttatg ttttaaaaaa taaaaatggg ggacaacaca tccctgctgc caaaattaga
     6841 gataatatct tggcccaaaa aacggattgt tgtcaatatc gtccctattt atagcaaatt
     6901 cgtgaacagc aacaaggggt gttaatggta ttttgaaaga aacaaagaaa atcactgctt
     6961 cgtcttagct tttagcgcca attcaatcat ttgttgtact actgccttag gttggttgtc
     7021 gcgatcgaaa agcagaggat agtctttgcg cccgcggacg gggaagttgt ttcgccagga
     7081 attgccgtcg ttcaggcccc agagtgtaac gcgatcgatg tgccggtggt gtttaagaaa
     7141 gagagcaaaa agttccgtga cacgttgttc ccaggctttc atcacctcgt cggggactcc
     7201 ctcgcggtaa gggtcgtttt gggtagaata aacaaagcgg tcggcaatat tggcccctgt
     7261 gtgccggtaa ggattaggca ggatggaaat gtcccattcg gtaatcagaa ccttgacgcc
     7321 ggtagcagcc agtttcagga tacttgtttc gaaagcttcc accgaaggtt cgtccatatg
     7381 cacgtgtccc tgcataccca cagcatcaat gcggcaaccg gcggctttga gttcttctac
     7441 catcttgaca acggcatcgc atttgaccgg attggccatg ttgtaatcgt tgtaatagag
     7501 ttcagcctcg ggatcggcct ggtgggcgta ttcaaaagcc agtcgaataa aatctttacc
     7561 cagaatacga taaaagggac tgttgcggta agtaccattg tcttcgaaag cttcgttgac
     7621 cacgtcgtag cctttgatgc ggcccttgta gcgagtcatg atgctgaaga tgtgctcccg
     7681 catcctcgat ttgagaactt cggcactaac cggctcaccc tgttcatcta cgcagaacca
     7741 gcgagccagt tgagaatgcc agatgagaca atgtccggtg acgacttgcc ggttgttctc
     7801 tccaaaagcg agcatttggt cggtggcttc gaagttgtaa cgatgttctt cgggatgaat
     7861 ctctgccgat ttcatgcagt tttcgggcac aatggcgctg aagtgcctgg agatttgttc
     7921 tgcttcggcc ggcctggcac cccgggtttg agggttgttg acggctacgc ctatcagaaa
     7981 aaggtcttcg gtagcctggc gcagagttgt ttgagccgtt aagtggctgc aaaacaacag
     8041 gacaactgta gtcagtagga ggaatgttcg ttgcatattt ttcaaggcta agaagtttgg
     8101 ttattaatac ggttgtatag aatcttttct ctgttggcct aagggtttta tagtcggttc
     8161 acctgttcat aaaataccgc atatccgttt ttttgagcac ttccagatcg tgaaccggcc
     8221 tttccatatc ttcggggaaa ggcagttggt tgatcgattg gaaatagagc aggcaggcgt
     8281 ctttccacac ctgggcatcc cgggcttgcc tgcggagttt ggcctgaacc tgggtaaaaa
     8341 cctcgctgtc aacccggttt tccatattat cccagaccag ttggaaacgg cggacttcct
     8401 gaaggccggc atcgtagcgc cggcagagtt cctcccacag actgttgccg ttccgcaggc
     8461 gatgttccca ggggaggtga tgaaaccaga gcagcagatt ttcgggacag ctgcccgggt
     8521 tgtcgaattg ggaacgcaag ggctcccggt attgcgacac ggcgttgctg ccccccgaac
     8581 tgcggtcaaa gcctatgcct ttagcatcgg cctgatgata atagggcgga gtccagtctt
     8641 ttctttgtct cggggagtcg taccaaggcc cgggcccgta gtgatggtcg agagcaaaca
     8701 agtggtgcag gcccaggggc atcatgtaat tgaccatcgc ctcgcgactc cggagcatca
     8761 tggataaaac cggttgaaga aattcttttt cccagttgcc gttcttctgg tccgaagccg
     8821 gatcgttgtc caaggccgga ctttcgtccg aaagcgaatt gcttttcggg gattgcgtgg
     8881 gttcaaaagt cagcctcagc cattcttcgg caatcccggc ggctgaggcc cggttgttcc
     8941 aagccagacg tccgaaagca taccagttgg caccggcaaa cggatggccg caccagttgc
     9001 tgtccgaacc gatgtttgaa accccggcta tggcagtgag ggattgatcg aaaaggctgc
     9061 cgtccgtaca gcgggctacg gtgctgcccg ggcctgactg ataggtgtcg ctttgcagga
     9121 attcttccca taaaccgccc aggtaagcca gttggtgttc ttgtcccagg tattcctggg
     9181 taatttgcag ttcgggcagg acggccgttt tccggagggc tccaaacaaa ggactgaagg
     9241 gctctctggg ctgaaaatcg atgggcccgt ttttaatctg aacaacgaca ttatcgcaga
     9301 attgtccgtc caaaggcatg aattcctcgt aggcttgttt ggcccggtcg ttgtcgctcg
     9361 gcttgtatac aaaggcccgc cacattaaga tgccgtggta gggcttgagc agttcggcca
     9421 gcatgttggc tccctgggcg tggctacgtc caaaatctcc cggtccgggc atgccttcgc
     9481 tgctcgcttt gaccagaaag cccccgaaat cgggaatcag ggaatagatt tcgcggattt
     9541 ttgcccgcca ccaatctacc acctccgggt caagcggatc ggccgttttg agtccgccca
     9601 gacgcatagg cgtagaaaaa ttaatgctca ggtagcttac cattccgtag gggcgcaact
     9661 cgtcggcgat agctgcagcg cgtttcagta cgggcaggct cagggcttcg ggcggggcat
     9721 tgacgttgtt taaaacggcc ccgttgatgc cgatagacgc atttattgca gcgtaggttc
     9781 gccagcgttc gcggtcttct gccgtcggct ccatcgactg cttgcccttc caaaagatgg
     9841 aacggccggc atagcctctt tcgatgcttc cgtccaggtt gtcccaatgg ttaagcaagc
     9901 gcctttggta ggagggattg gaaaagggca gttctaaact gagaccgatt tgccggcgtc
     9961 gcaaatattc ataggctccg tagagcaaac cttgatgtgt tttggccgtg atgcagccgg
    10021 gcgataaact gtaaccttgt tcggacagtc ttttatcgcg gctgatcttt aatttgatgc
    10081 tgttgttttc ccgatcctgc cagccatcga tcaagacttg tttagccagc tccagagtag
    10141 gagagctttt tccggacggc atatcgactt tgactgctga cagggcagtt gccggcaacc
    10201 agagctctct gcctgtttca gcctgcaacc ggaatgctcc tgcaacaagc agtaaaaaga
    10261 aacaatatag gataaattta ttatgcatgc ttttagagga agaaccttcg tgttgcaaga
    10321 tacggtaaaa gactttattt ttaaaaacaa agatttaaaa atcctgattt atagttgttt
    10381 catcggtaag ttgggaatca attgccacct ttctacggag ccgatcagga ataaataaat
    10441 tgaaatcagg aataaaaaaa gcccactctt tttaaagtgg gctattatct tttttgtggt
    10501 accaccagga atcgaaccgg ggacacaagg attttcagtc ctttgctcta ccaactgagc
    10561 tatggtacct tttccttttg gaagcggctg caaaagtaca attcattttt aaaacaacaa
    10621 agactttgcc gaattatttt gtaaaaaacg ggctatttgt ttgtacatag aaaaaagctt
    10681 ttctaccttt gcacggtaat ttcaatcggg atgtagcaca gttggtagcg cgctacgttc
    10741 gggacgtaga ggtcggaagt tcgagtcttc tcatcccgac caaaaggcac ttaaacgtag
    10801 aattttccgc tttaaagtgc cttttgttta tataaaatcg aaacaatgct tacactaaag
    10861 tacctcagcg aaaacaccag agaatgtata gagcgtctgg ctgttaaaca ttttgatgca
    10921 gaagcgctta tcggagaaat tcttgagctc gatttgcaac gtcgcaatgc tcagacccgg
    10981 tcggatgcct gcgcagcaga attgaatact ctttccagat ccataggcgt gttgatgcag
    11041 gaaggcaaaa aacaggaagc tgccggagtg cgtgaaaaaa ccctgcaact caaagaagaa
    11101 ggcaaaaggc tcgaaatcgc acgcaaagag gccgaaaaca ggatacagga gctcttggtg
    11161 caaatcccca atcttccgca tagttcagtg ccggccggca aaggagccga agacaatgtg
    11221 acggaacgct ccggcggccc tatgcccgac ttgccggtcg atgccttgcc tcattgggac
    11281 ctggccaaaa aatacgattt gatcgatttt gaattgggag ccaaaattac cggagccggt
    11341 tttccggttt acaaaggcaa aggagcccgt ttgcaaagag ccctgataaa ctatttcctc
    11401 gatcgcgcac ggcaggcagg atacctcgaa attcagccgc cttatgtggt aaacgccgct
    11461 tcgggttatg gtacggggca attgcccgat aaagaggggc agatgtatca tgtcgggttg
    11521 gatgatctgt acttaattcc gacagccgaa gtaccggtaa ccaatattta cagggacgtc
    11581 attttggacg aaaaggacct gccggtaaag aatactgcct attcggcttg tttccgtcgc
    11641 gaagccggag cttacggcaa ggacgtcagg ggactgaatc gcctgcatca gtttgataag
    11701 gtggaaattg tctgcataga acatcccgat cgttcttacg aacgtttgga aaaaatggtt
    11761 gcccatgtgc aaagtctggt cgaagcgttg gaattgccct ggcgcatttt gcgcctgtgt
    11821 gggggcgata tgggctttac ctcggctctg acttttgatt ttgaggtcta ttctgccgcc
    11881 cagcaacgct ggctcgaagt gagctcggtg tcgaatttcg aaagttttca ggccaacagg
    11941 ctcaaatgcc gttaccgtac tgccgaggga ggtaaaattc agttgtgnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnngcc aggtctttgg ctttttcttc ccatactttg
    12121 ccgtcggcgt aggtgtcctt gggatcaaga atagccggat ttacgttggg caaagcggtg
    12181 gggaccttaa agttgaagta agctatttct ttggtggctg ctttgtcgat ggagccgtcc
    12241 aggatagcgt cgatgatgcc gcgggtatct ttgatggtaa tacgtttgcc ggtaccgttc
    12301 caaccggtat taaccaggta agcggtggct ccggaagctt gcattctctt aaccagttcc
    12361 tgagcatact tggtaggatg caaggccagg aaggctgctc caaagcaggc agagaaagtg
    12421 ggagtaggct cggtaatacc acgttcggtt ccggccagtt tggcggtgaa accgctcagg
    12481 aagtagtatt gggcctgttc gggggtaagt atcgacacgg gaggcagtac gccgaatgcg
    12541 tcggccgaca ggaagattac tttcttggca ggaccggctt tggatacggg ttttacaatg
    12601 ttgtcgatgt ggtaaatcgg gtaagaaaca cgggtgtttt cggtaacact gccgtcgtta
    12661 aagtcgattt tgccattggc atcaaccgtg acgttttcga gcagagcatc gcgacgaatg
    12721 gccttccaga tgtcgggttc ctgttctttg ctcagattga tggttttggc gtagcagccg
    12781 ccttcgaagt tgaatatgcc ttcgtcgtcc cagccgtgtt cgtcgtcgcc gatgagctga
    12841 cgcttgggat cggtagaaag ggtggttttg ccggtaccgg acaagccgaa gaagatggcg
    12901 gtatcgccgt ctttccctac gttggccgag cagtgcatgg aagctatccc ctgcagaggc
    12961 aggtagtagt tcatcagggt gaatatgcct tttttcattt ctcccccgta ccatgttccg
    13021 ccaatcacct gcattttttc ggtcaggtta aaaacagtga aagtttcgga attcaatccc
    13081 agttctttgt attgagcgcc ggcacaggtt ttggatccgt tgagggatac aaaatcgggt
    13141 tcgccgtaat ttttgagttc ttcttcggta ggacggatga acatgttctt tacgaagtgg
    13201 gcctgccagg ctacttccat gataaaacgg actttaagac ggctggcttc gttggcgccg
    13261 cagaaagtgt ctaccacata gagttttttg ttggataatt gcttggtaac gatgtctttc
    13321 aatacacccc aggatgcctt gggcaggggt ttattgtcgt ttttatattc gtcggaagtc
    13381 caccagatgg tgtccctcga aacatcgtcc atcacgatga atttgtcttt aggtgagcgt
    13441 ccggtataga cacccgtcat cacattgaca gcccccattt ctgtaacctg tcccctttcg
    13501 aatccctgta aattgggatc ggtctcatct ttgaataatt gctcatagga agggttgtga
    13561 acaatctcgg tagtaccttt gattccgtac tttgttagat ctaatgattt catttttaaa
    13621 tgattaatag tgaatttgtt gaaaactttt tatagggcca aattttatcg ggcgcaaaga
    13681 tagaaaataa aatcagatgc tgctaattaa ttaaagaaaa atttcagcaa ttgatgaaaa
    13741 tattttttag gtttcccctc tttcgttttc ttattccgtc agcggaggga ttgagctctc
    13801 ggttgctgat ttttttgtct tctatccgtt cgtacatcgt caggcagaga tcgaaaatgt
    13861 tgtccgggct tggttgaggg ggagttgcgg tggcaggaaa ctccaaggct ctggttttca
    13921 gtgttttcgc aggaatttgt tcaaaaactg caaactttgc tcgtgcatgg cccggctgaa
    13981 aaaatgaggg gaatcccaga tttctgtcct cagacagtct ccggctccct ggctttccca
    14041 gacccggcgc atggtttcga aagcatccag acaaccttgt acagggaaga gcttatcccg
    14101 gctgccgttg atgaaataca tgggcttggg gcaggcgatg gaagctacgt ggggataatc
    14161 caggtaattc ctgatttcgg gcagcaacat cgaataggcg ctgcctccct tgttttggtt
    14221 gttgtcgagt gtcataaggt gttcggtggt gttcatccag cagacggcaa cagcggcgct
    14281 gactttgtcg ctggcggccg ccagcatcca ggcccggtgg gctcccatgg aaaagcctaa
    14341 tgcggctata cgttctgtgt ctacgtaggg aaggctgcac aaaaattcca cactttgaat
//
LOCUS       DAOSTD010000042        14174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_4367_length_14174_cov_16.392096, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000042 DAOSTD010000000
VERSION     DAOSTD010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14174
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4367_length_14174_cov_16.392096"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLI37_04275"
     CDS             complement(<1..727)
                     /locus_tag="PLI37_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOQ58262.1"
                     /translation="MMTERIKQLRRESLEAINTINAERALLITEFYSKHLAFGDSVPV
                     QRAKSLQYILRNKSICINENELIVGERGPAPKATPTYPEINVHSLKDLDILNSREKVW
                     FRVDKETRKAYEDIIIPFWKGRCNRDRIMESMSPEWLNAYRAGIFTEFMEQRAPGHTV
                     AGYKLFQKGMLDILEDIKQSRSNIDPLNDPAAYDKLEELKAMEIAAHAIMEYAERHAQ
                     KLEDLAAVEKNDQRRRELQQMAKI"
     gene            complement(748..>1077)
                     /locus_tag="PLI37_04280"
     CDS             complement(748..>1077)
                     /locus_tag="PLI37_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58263.1"
                     /translation="HFTTVSNKLILDNLIYLLENRHPLRIRIPVIPEINFNQENTDEC
                     LRFLAAHPGNIQGVDLLPYHNMANHKYLRFKKENSLSSLQSLPKSSLIAMKEQFEQAG
                     YETKIGG"
     assembly_gap    1078..1177
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1178..1368)
                     /locus_tag="PLI37_04285"
     CDS             complement(<1178..1368)
                     /locus_tag="PLI37_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOQ58264.1"
                     /translation="MQGLVFDIKRFAVHDGPGIRTTLFLKGCPLNCAWCHNPEGTSPE
                     PQTVAKSISVDGQVFQEEE"
     gene            complement(1370..2683)
                     /locus_tag="PLI37_04290"
     CDS             complement(1370..2683)
                     /locus_tag="PLI37_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOQ58265.1"
                     /translation="MKYLFPLLFFVLIACSGSSNQPYSQKMVESLSLRTFNNNRNTST
                     LQQADFSYVPGLVAKSVLKTFELYPDKRPYYESVKAYADRQLRDDPAAPLKIGDDDID
                     AINAGKIFFDLYKQSVKDGDTESADKYKAAATFMYNKLKNEHTRILAPKPGAGGFIHK
                     GRYPDQMWLDGLYMGAAFYAEWEATFGESGNTESWSDIAYQFKTIHQYTWDSEKQLNY
                     HAWSASPTDSNSFWANQQEPFKGASKEFWGRGSGWYFAALVDVLELMPKEHADYKALQ
                     DIYTQVALGLQRWQDKKSGCWFQLLQYDETTTADGLGDLVNGRRYNIGDLPNYLESSA
                     SAMFCYGMAKGVRLGLLDKESFMPTVEKAYQGLIDQFITLNPDQSINIIQSCASAGLG
                     PARNPSRTGTINYYLCGSDVTITQNEGKAIGSFTMASCEYERLKN"
     gene            2873..3733
                     /gene="fabD"
                     /locus_tag="PLI37_04295"
     CDS             2873..3733
                     /gene="fabD"
                     /locus_tag="PLI37_04295"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOQ58266.1"
                     /translation="MKAYVFPGQGAQFTGMGKDLYDSFKEAKAYFELANKTLGFDLVQ
                     IMFEGSDDELKQTKVTQPAIFLHSVVAAKCLPDFKPDMVAGHSLGEFSALTAAGALAF
                     EDGLSLVYKRAMAMQAACEKNPSTMAAVLGATDELAEQVCASIESEIVVPANYNCPGQ
                     IVISGSFKGVELASEQLKAAGAKRVLPLKVGGAFHSPLMESAAAELQAAINQTKFSRP
                     CCPVYQNVDAKPYTDPVAIQKNLIAQLTSPVRWTQSVVAMLRDGAEEFIELGPGTVLQ
                     GLIAKIRANQ"
     gene            3735..>3991
                     /locus_tag="PLI37_04300"
     CDS             3735..>3991
                     /locus_tag="PLI37_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HOQ58267.1"
                     /translation="MAKLIIYQVFPRYFGNNRRNSVSNGDIEDNGTGKFNDFSAKALN
                     AIKELGVTHIWYTGVLEHATQTDYSSYGIKSDPPELVKGKAG"
     assembly_gap    3992..4091
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4092..5300
                     /locus_tag="PLI37_04305"
     CDS             <4092..5300
                     /locus_tag="PLI37_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOQ58268.1"
                     /translation="NNFYYLPGQSFRPPQEALIPGRKVQYTEKPAKVTGNDCFKAQPS
                     VNDWYETIKLNYGVDYQGGNLKYFDPIPDTWHKMKAVLEYWVKLGVDGFRCDMAEMVP
                     LEFWAWVIPQIKKIKEVLFIGEVYNPGLYESFVRRGGFDYLYDKVGLYDTLRNVICEY
                     APTSAISDCWKRIGSLQDNMLNFLENHDEQRIASDFFAQDPRKALPALIVSAYMHNNP
                     FMLYSGQELGEKGMYREGFSGLDGRSSIFDYWCIDSLADWNNGGRFDGALLSAEQKEL
                     RNNYAKILNLCSQEPALYRGLFFDLMYVNMDNPYFDFSRQFAFLRKADRELLLIVVNF
                     DAESKHIRLRIPEHAFEYLRIKPQKQWLGRDLLSGEELPFELNTQDPLPMMLPAHYGR
                     IWKYRMEDRE"
     gene            5399..>6210
                     /locus_tag="PLI37_04310"
     CDS             5399..>6210
                     /locus_tag="PLI37_04310"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOQ58269.1"
                     /translation="MRLKGPIKIFSGTKSHYLAEKICAALECPLGRMNIQHFADGEFS
                     VSYEESIRGDYVFLVQSTFPNSDNLMELLLMIDAAKRASAGKIIAVVPYFGWARQDRK
                     DKPRVSIAAKLIADILSVAGIDRLITMDLHADQIQGFFDVPVDHLYASTIFLPYIRSL
                     NLDNLVIATPDVGGSKRANSYSKYLGVPMVLCHKSRNKPNEVAEMRIVGDVAGKNVLL
                     VDDMVDTAGTLTKAANLISSEGALSVRAVASHCVMSGKATELVENSELTEVI"
     assembly_gap    6211..6310
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6311..7132)
                     /locus_tag="PLI37_04315"
     CDS             complement(<6311..7132)
                     /locus_tag="PLI37_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOQ58270.1"
                     /translation="MKKLVFLIAAGLLFACNQADKSKLDFGTAQGDLGNGYFLNPILG
                     GDYPDPTIMREGKDYYMTHSSFDYVPGLVVFHSTDLVNWQPISYALKRYLGSVWAPDI
                     CKHEDRYYIYFTVAHPSGRMNFVTYADSPYGPWSDPIDLKVGNIDPGHAVGEDGSRWI
                     FLSGGQRARLNDDGLSIVPGSLEKVYEGWIYPSDWVTEGMCLEGPKIRKIGEYFYYLN
                     AEGGTAGPPTSHMVVAARSKSINGPWENSPYNPIVRTWQASERWWSKGHGSIIDSP"
     gene            complement(7144..8688)
                     /locus_tag="PLI37_04320"
     CDS             complement(7144..8688)
                     /locus_tag="PLI37_04320"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOQ58271.1"
                     /translation="MAQQEDVFKKLISHCKEYGFVFPSSEIYDGLSAVYDYGQMGVEL
                     KNNLKKYWWDSMVLMHENIVGIDSAIFMHPTIWKASGHVDAFNDPLIDNRDSKKRYRA
                     DVLIEEQLAKYDDKIDKEVAKAAKRFGEAFDEALFRVTNPRVVENLQKKEVLHQRFAE
                     ALNQNDLEALRQIILDEEIVCPVSGSKNWTEVRQFNLMFSTEMGSTADGAMKVYLRPE
                     TAQGIFVNYLNVQKTGRMKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVKP
                     GTELEWFEKWKEIRLKWHKALGLGDHKYRFHDHEKLAHYANAATDIEFQLPFGFKEVE
                     GIHSRTDFDLSNHEKYSGKSIKYFDPELNESYVPYVVETSIGLDRTFLSILAGSYEEE
                     TLPGGESRVLLHLPPALAPIKLAILPLVKKDGLPEKARQIIDELRFDFKCQYDEKDSI
                     GRRYRRQDAIGTPFCATVDHDTLQDNAVTVRDRDSMEQIRVPIEELRSLISDKVDLKQ
                     LLKKQA"
     gene            8939..>9264
                     /locus_tag="PLI37_04325"
     CDS             8939..>9264
                     /locus_tag="PLI37_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOQ58272.1"
                     /translation="MYRNWFLDYASYVILERAVPHIDDGLKPVQRRILHSMKRLDDAR
                     YNKVANIIGHTMQFHPHGDASIGDALVQLGQKELLIDCQGNWGNLLTGDGAAAPRYIE
                     ARLSKFA"
     assembly_gap    9265..9364
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9365..11389
                     /locus_tag="PLI37_04330"
     CDS             <9365..11389
                     /locus_tag="PLI37_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOQ58273.1"
                     /translation="QTGGYIDVSRYNDGERGGSVKVRAKISKLDNKTLVITEIPFGKT
                     TSGIIDSILKANEKNKIKIRKVDDNTAQNAEILIHLASGTSSDKTIDALYAFSDCEVS
                     ISPNCCVIADEKPQFLTVSDVLRYSADNTKELLKKELLIRKGELLESLLFASLEKIFI
                     EERIYKDKAFEESTSMDKALSHIDKRLEPFKKDFVREVKRDDLLRLTEIKMARILKFN
                     IDKAEEAIVAMQEQIKEIDHHLATLVEYTIAWFESLKKKYGAAFPRKTEIRSFDTIEA
                     AEVVEATEKLYINREEGFIGTSLKKDELVCPCSNLDDVILFYKDGKYKVVKVSDKMFV
                     GKNVLYLNVFKKNDTRTIYNVIYRDGKFDNKGGGVSYIKRFAVSGVTRDKEYDLTKGT
                     PGSRIQYFSANPNGEAEIVRVTLKPAPRIRKLQFEKDFGEIAIKGRQSMGNILTKNDV
                     FKIALKQKGGSTLGGREVWFDPDVLRLNYDGRGEYLGEFHAEDSILVMTKSGEYYMTN
                     IDLSNHYEDNILRIEKFEEDKIWTAALFDAEQQNYPYLKRFPLEFSLRKLSFTGENPE
                     SRLILLSDTYYPRIEVSFGGDDSHREKLVLDCDEFIGTKSFKAKGKRITTYEVAEIKE
                     IEPLRFPPPKEDDNNSEPEAVIVSESDESEEDNNPGKLIDEITGQMTLFD"
     gene            11425..11506
                     /locus_tag="PLI37_04335"
     tRNA            11425..11506
                     /locus_tag="PLI37_04335"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11459..11461,aa:Leu,seq:tag)
     gene            11992..>14174
                     /locus_tag="PLI37_04340"
     CDS             11992..>14174
                     /locus_tag="PLI37_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ58274.1"
                     /translation="MKPTCRTLFFTLLLSIMASGSVCSGNWNHFNNNWKFTKGNPENA
                     QSTNFDDSSWEKVNLPHDWAIKGPFNEQGNGSTGKLPWRGEGWYRNTFTLSSEDQGKV
                     VYIKFDGVMSSPQIFVNGKLAGKWDYGYSSFYLDISDYVKYGQENSVAVYVDNRKHDS
                     RWYPGAGIYRKVEMMTTEKVHEEIWGTYITTPVVSQSYAEMRILSNVWNKDSEDKKVT
                     VEAVLVSPEGFEIKTVASRQRNLPAGSLFQFDKWMTVVRPQIWDVDHPVLYSLKTKVI
                     VDGKVVDSNETPFGFRSFEFTADDGFHLNGRRLQLRGVNLHHDHGPLGAAFNKRSMER
                     KLEILKEMGCNAVRTSHNICAPELIELCDKMGLLVFNEAFDKYDKKADIFEATDFYEF
                     GERVIKNFVLRDRNNPSVFIWSVGNEMGDIQSNSNFGLQRLAAMVGFVRKYDITRPVT
                     MACDQESNAHWRHFDYYDVHAWNYGQRWKTARKLDPSKSVIISESASTVSTRGFYELP
                     LPEKPTDFTQSNQVSSYDLHAPSWAEVCDDDFMWQENDKYIAGEFVWTGFDYLGEPTP
                     YSSSRSSYFGIVDLCGIPKDRYYLYQSYWRPEKDLIHILPHWNWPGHEGKKVPVFVYT
                     NGDEAELFLNGKSLGKQSRKPKSEISTERYRLMWHDVVYEAGELKVVAYRVGKVIGEK
                     VLRTAGEAYTLKLTPDRSRLKASGEDLSFILVEAYDKNGNLCPLA"
BASE COUNT         3675 a         3042 c         3389 g         3668 t
ORIGIN      
        1 agatttttgc catttgttgc agttcccggc ggcgctgatc gttcttttcg acggcagcca
       61 gatcttccag tttttgggca tggcgctcgg cgtactccat aatggcatgg gccgcaattt
      121 ccatggcctt gagttcttcc agcttgtcgt aggctgccgg atcgttcagg ggatcgatgt
      181 tagaacggct ttgttttatg tcttcgagga tgtcgagcat gcctttttga aacaacttat
      241 agcctgccac cgtgtgtccc ggagcccgtt gttccataaa ttccgtaaat atgccggccc
      301 gataagcatt tagccattca gggctcatgc tctccataat gcggtcgcgg ttacaacgtc
      361 ctttccagaa tggaatgata atgtcttcgt aagctttccg ggtttcctta tccacacgga
      421 accagacctt ttctctggaa ttaaggatat cgagatcctt cagcgaatga acattgattt
      481 cggggtaagt gggagtagct ttgggagccg gcccgcgttc gcctacaatc agttcgtttt
      541 cgttgatgca gatgcttttg tttctcaaaa tatattgcag agacttagct cgctgaaccg
      601 gtactgagtc gccaaatgcc agatgtttgc tgtagaattc ggtgatcagt aaggctcttt
      661 cggcattgat ggtgtttata gcttccaggc tttcccggcg aagttgttta atacgttcgg
      721 tcatcataaa tgttaaatgg ataaatgtca acctccgatt tttgtttcgt atccggcttg
      781 ctcaaattgc tccttcatcg ctattaaact cgattttggc agactttgca aggaactcag
      841 cgaattctct tttttaaatc gcaggtattt gtggttggcc atgttatggt aaggtagcag
      901 atccactccc tgaatattgc cgggatgggc tgctaaaaaa cgcaggcatt cgtcggtgtt
      961 ttcctgattg aaattgattt cgggaatcac aggaatgcgt attcttagag ggtgccgatt
     1021 ctcgagcaaa taaatcagat tgtcgagaat aagcttgttg gagacagtcg tgaaatgnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttc tcttcttctt gaaacacttg
     1201 tccgtccacg ctgatgcttt tagcgacggt ttgaggttca gggctggtac cctccggatt
     1261 gtgacaccag gcgcaattaa gtggacagcc tttcaaaaac aaggttgttc tgatgcccgg
     1321 accatcgtgg acggcaaaac gcttgatgtc gaaaaccagc ccttgcatat tagtttttca
     1381 aacgttcgta ctcacaggaa gccatggtga acgaacctat ggccttgcct tcgttctgag
     1441 tgatggtaac atcgcttccg caaaggtaat aatttatggt accggtgcgt gagggattac
     1501 gggccggtcc aagccctgcg gaggcacaac tctgaatgat attgatgctt tggtccggat
     1561 tgagggtgat aaactgatca attaagccct gataggcttt ttcgaccgtg ggcataaagc
     1621 tctctttgtc gagcaagccc agtcgtacac ctttggccat gccgtaacaa aacatagcgg
     1681 aagccgatga ttccagataa ttaggtaagt caccgatatt gtaacgtcgc ccgtttacca
     1741 ggtcgcccag gccgtcggct gtggtagttt cgtcgtattg caacagttga aaccaacaac
     1801 cggatttttt atcttgccag cgctgcagac ccaaagccac ctgcgtataa atatcttgca
     1861 aggccttgta gtcggcatgt tctttgggca tcagttccag cacgtcgacc agtgcagcaa
     1921 aataccagcc ggaacctctg ccccagaatt ccttcgaggc tcccttgaaa ggctcctgct
     1981 gattggccca gaaagaattg gagtcggtgg gagaagcaga ccaggcatgg taattcagct
     2041 gcttttcgct gtcccaggtg tattggtgta tggtcttgaa ctggtaagcg atgtcggacc
     2101 aggactcagt attgccggac tccccgaaag tggcttccca ctcggcataa aaggcggctc
     2161 ccatatacaa tccgtccaac cacatctggt cgggataacg tcctttgtgt ataaagcctc
     2221 cggctccggg cttgggtgcc agaatccgcg tatgttcgtt ttttagttta ttgtacataa
     2281 acgtggcagc cgccttgtat ttatcggcgc tttcggtatc gccgtctttg acagactgtt
     2341 tatacaggtc aaaaaagatt ttgccggcgt tgatggcatc gatgtcgtcg tcgccgattt
     2401 ttagcggagc agcaggatcg tctctgagct ggcggtcggc ataggctttg acactctcgt
     2461 agtagggtct tttgtcggga tataattcaa aggttttcaa tacacttttt gcgactaagc
     2521 cggggacata gctgaaatcg gcctgttgta atgtgctggt gttcctgttg ttgttgaatg
     2581 tgcgcagact aagcgattcg accatttttt gcgaataggg ttggtttgaa cttccgctgc
     2641 aggcaatcag gacaaaaaat aaaaggggaa agagatactt cataatatgt tttattctaa
     2701 aagtgaataa tggttgaaaa tcagatgatt aatcgttttt tgcctttaag tccggtcata
     2761 aattggaaga ttcaacatac tagcaggcaa ttctaccggg cagtaaaaat acgaaaaaat
     2821 ctgcattcct ttaaaattat gtatttttgc cccgaattat tacgaccaaa ttatgaaagc
     2881 atacgtattt cccggacagg gagctcagtt taccggtatg ggcaaagacc tgtacgacag
     2941 tttcaaagaa gcaaaagcgt actttgaatt ggccaacaaa accctgggtt ttgatctggt
     3001 gcaaatcatg tttgaaggtt cggacgatga gttgaagcag actaaggtga ctcagcctgc
     3061 tatctttttg cattcggtag tagcggccaa gtgtttgccc gatttcaaac ccgatatggt
     3121 ggccggacat tctttagggg aattttcagc acttacggca gccggagctt tggcttttga
     3181 agacggtttg agtctggttt acaaaagagc catggccatg caggccgctt gcgaaaaaaa
     3241 tccttctact atggccgccg tgttgggcgc cacggacgaa ttggcagaac aggtctgtgc
     3301 ctccatagaa tcggagatag ttgttcctgc caattacaat tgccccggac aaatagtaat
     3361 ttcgggcagc ttcaaaggag tggagctggc ctcagagcag ctaaaagccg ccggtgcaaa
     3421 gcgcgtgctg cctctcaaag tcggcggggc ttttcattcc ccattgatgg agagtgctgc
     3481 agcggaattg caggcagcca tcaatcaaac aaagttcagc cggccttgtt gtccggtgta
     3541 tcaaaacgtc gatgccaagc catatacaga tcccgtcgcc atacaaaaaa atttgattgc
     3601 tcaattaact tctcccgtgc gttggacaca aagcgtggta gctatgttga gagatggtgc
     3661 cgaagaattt attgaactgg gtccgggcac tgtattacaa ggattgattg ccaaaatcag
     3721 agccaatcaa tgatatggct aaactaatta tttatcaggt gtttcctcgc tattttggaa
     3781 acaatcgcag aaactctgta agcaacggag acatcgaaga taacgggacg gggaagttta
     3841 atgactttag cgccaaagcc ttaaacgcca tcaaagaatt gggagtgact catatttggt
     3901 acaccggggt tctggaacat gctactcaaa ccgattattc aagctacggc ataaagtccg
     3961 atcctcccga actggtaaag ggtaaggccg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn naataatttt tattatttac ccggacaaag ttttcggcct ccccaggaag
     4141 cgctgattcc cggtagaaag gtgcaataca ccgaaaaacc tgccaaagta acaggcaacg
     4201 attgttttaa ggctcaaccc tcggtcaacg attggtatga aaccataaaa ttgaattacg
     4261 gcgttgatta tcagggagga aatttaaaat attttgaccc gatccccgac acctggcaca
     4321 aaatgaaagc cgtactggaa tactgggtaa agctcggtgt tgacggtttt cgttgcgata
     4381 tggccgaaat ggtgcctttg gagttttggg cctgggtaat tccgcagata aaaaaaatca
     4441 aagaagtcct tttcataggc gaagtgtaca atccgggctt gtatgagtct tttgttcgaa
     4501 gaggcggttt cgattatttg tacgataagg tggggcttta cgatacctta cgcaatgtta
     4561 tttgcgaata cgctccgacc agcgctatat cggattgctg gaagcgaatt ggctccttgc
     4621 aggataatat gttgaatttt ctggaaaatc acgacgaaca gcgtatagcc tccgattttt
     4681 ttgcccaaga tcctcgcaaa gctttgccgg ctctgatcgt gtctgcctac atgcataata
     4741 atcctttcat gctttattcg ggtcaggaac tgggcgaaaa aggcatgtac cgggaaggtt
     4801 tcagcggatt ggacggacgc agcagtattt ttgactattg gtgtatagac agtctggctg
     4861 attggaacaa cgggggacga ttcgacggtg ctttgctgag tgcagaacaa aaagagttgc
     4921 gcaacaatta cgctaaaatc ttgaatttat gctcgcaaga accggctttg tacagaggct
     4981 tgttttttga cctgatgtat gtgaacatgg acaatcccta ttttgatttc tcccgacagt
     5041 ttgctttttt gcgtaaagca gaccgggaac tcctgctgat agtggttaat ttcgatgccg
     5101 agagcaaaca tatccggctc cgcatacccg aacatgcctt tgaatattta cggattaaac
     5161 ctcaaaagca atggctgggc cgggatttgc tgagtggaga agagctgccg tttgaattga
     5221 atacgcaaga tcctctaccc atgatgttgc cggctcatta cggaagaata tggaaatata
     5281 gaatggagga ccgagaatag ccggggagat ttttttcgga cattactccg atacttgaaa
     5341 gcaaatgtct atctttgtgc tcaatttata acctcaccac ttctcaagct taagatctat
     5401 gcgtttaaag gggcctatca aaatcttttc aggaaccaaa tcacattatc tggccgaaaa
     5461 aatatgtgcg gccttggaat gtccgctggg gagaatgaat atccagcatt ttgccgacgg
     5521 ggaattttcc gtctcttacg aagaatccat acgaggggat tacgtctttt tggtgcagtc
     5581 tacctttcct aattccgata atttgatgga gttgctgctg atgattgatg cagccaagcg
     5641 ggcttcggcc gggaagataa ttgctgtagt accctatttt ggctgggcca ggcaagatcg
     5701 caaggataaa cccagggtgt cgattgctgc caaactgatt gcagatattt tgtctgtggc
     5761 cggtatagac cgtctgatca ctatggatct tcatgccgat cagattcagg gctttttcga
     5821 tgtgcccgta gaccatttat acgcctctac catttttctt ccctatatcc gctctctaaa
     5881 tctggataat ttagttattg ccactccgga cgtgggaggt tccaaaagag ccaattcata
     5941 ttccaaatac cttggtgtac ccatggtact ttgtcacaaa tcgcgcaata agcccaacga
     6001 agtggccgag atgcgcattg tgggagatgt cgcaggcaaa aatgtccttt tggtagacga
     6061 tatggtcgat accgcaggta ctcttaccaa ggcagccaat ctgatatcga gcgaaggtgc
     6121 cctgtctgtg cgggcagtgg caagtcattg tgttatgtcg ggcaaagcca ccgagttggt
     6181 cgaaaattca gaactcacag aagtgatttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn gggggaatct atgatcgatc cgtgtccttt ggaccaccag cgttcggagg
     6361 cttgccaggt gcggacaatg ggattgtagg gagagttttc ccaagggccg ttgatcgatt
     6421 ttgagcgtgc ggcaaccacc atgtggctgg taggcgggcc ggcagtgcca ccttcggcgt
     6481 tgaggtaata aaaatactcc ccgattttac gaatcttggg gccttccagg cacatgcctt
     6541 cggtgaccca atcggatggg taaatccaac cctcgtacac tttttccaga ctgccgggaa
     6601 caatcgaaag accatcgtcg ttgagtctgg ccctttgccc tccgctcagg aagatccagc
     6661 gcgatccgtc ttcgccgacg gcatgtccgg gatcgatatt ccccactttt aaatcaatgg
     6721 gatcgctcca ggggccgtag ggagaatcgg cgtaagtgac aaaattcatt ctgcccgaag
     6781 gatgagccac agtaaaatag atgtaatagc gatcttcgtg tttgcagata tccggagccc
     6841 agactgagcc cagatagcgc ttcaaggcgt aactgattgg ttgccagttg accaggtcgg
     6901 tggaatgaaa tacaaccagg ccgggaacgt agtcaaacga agaatgggtc atgtaataat
     6961 ccttaccctc cctcataatg gtcggatcgg ggtaatctcc gcccaatatg ggattgagaa
     7021 aatacccatt gcccaggtct ccttgagcgg ttccgaaatc cagtttgctt ttgtcggcct
     7081 gattgcaagc gaaaagcagg ccggcggcta tcagaaatac taattttttc atggtaggtt
     7141 tgtttaagct tgttttttga gtaattgttt cagatcgact ttgtcggaaa tcagcgatct
     7201 cagctcttcg atgggaaccc gaatttgttc catgctgtcg cgatcgcgca ccgtaacggc
     7261 gttgtcttgc agtgtgtcgt ggtcaaccgt ggcgcaaaaa ggcgttccga tggcatcttg
     7321 tctgcggtag cgcctgccga tggagtcttt ttcgtcgtat tggcatttaa aatcgaagcg
     7381 aagctcatcg atgatctgac gggctttttc gggcaggccg tcttttttga ccaggggcag
     7441 tatggccagc ttgatgggcg ccagggccgg aggcaggtgc agcaacaccc ggctttcgcc
     7501 tccgggcagc gtttcttctt cgtaagaacc ggccagaata ctcaggaagg tgcggtcgag
     7561 tccgatagaa gtttctacga catagggcac ataggattcg ttgagttccg ggtcgaaata
     7621 cttgatgctt ttgccggaat atttttcgtg gttgctcagg tcgaaatcgg ttctggagtg
     7681 gataccctcc acttccttga agccgaaagg caactggaat tcaatatcgg tggcggcgtt
     7741 ggcgtaatgt gccagtttct cgtggtcgtg aaagcggtat ttatgatcgc ccaggcccag
     7801 agctttgtgc catttcaggc ggatttcctt ccacttttca aaccactcga gttcggtgcc
     7861 gggtttgaca aaaaactgca tttccatttg ttcaaactcc cgcattctga aaataaactg
     7921 gcgggcaaca atttcgttgc gaaaagcttt accgatctga gcgatgccaa aaggtatttt
     7981 catccttccg gttttttgaa cgttgaggta attgacaaaa ataccctgag cagtttcggg
     8041 ccttaaatag accttcatgg cgccgtcggc agtggaaccc atttcggtgg aaaacatcag
     8101 gttaaactga cgcacctccg tccagttttt gctgccgcta acaggacata caatttcttc
     8161 gtccaatatt atctgacgca gtgcctccag gtcgttttgg ttgagagctt cggcaaagcg
     8221 ctgatgtaga acttctttct tttgcaggtt ttcgacaact ctgggattgg taactctaaa
     8281 cagggcttcg tcaaaggctt ctccaaaacg tttggcagct ttggctactt ctttatcgat
     8341 tttatcgtcg tacttagcca gttgttcttc gatgagcaca tcggcgcggt agcgtttttt
     8401 ggaatccctg ttgtcgatga gtgggtcgtt gaatgcgtct acgtgacccg aagccttcca
     8461 gatagtcgga tgcataaaga tggccgaatc gataccgaca atattttcgt gcatcagcac
     8521 catagagtcc caccaatatt ttttcaggtt gtttttgagt tcaactccca tttgaccgta
     8581 atcgtaaacg gccgacaatc cgtcgtaaat ttcgctggaa gggaaaacaa aaccgtattc
     8641 tttgcaatgt gaaatcagtt tcttaaaaac atcttcttgt tgtgccatag ctgctttttt
     8701 ttagtttgca aaggtagggt ttaatttgtt tttatgcaca tcggacgaaa aaaatcgtat
     8761 ctttgcggct taaaacaact caacactata cccttatatg gaggaagaac atttgctgaa
     8821 tgagactgat gttccggaca atttagatgg tcggaccaga gcagacaaag ataaagacaa
     8881 aaatacgctc tcgcctgact cgctcaaccc tgcccacaca aagcaacaat taacaggaat
     8941 gtaccgcaat tggttcctgg attatgcttc gtatgtgatc cttgagcgtg ccgttcccca
     9001 tatcgacgac ggtctgaagc ctgtgcagcg tagaattctg cactccatga aacgtttgga
     9061 cgatgcccgt tacaacaaag tggcgaatat tattggccat accatgcagt ttcatcctca
     9121 cggcgatgcc tccataggcg atgctttggt gcagttgggg caaaaggagt tgttgatcga
     9181 ttgccaggga aactggggca acctgcttac cggagacgga gctgcggctc cgcgttacat
     9241 agaagccagg ctgtccaaat ttgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnncaaacc ggtggttaca tagatgtcag tcgctataac gacggagaac gcggcggtag
     9421 cgttaaagta cgggccaaaa tcagcaaact cgacaataaa acattggtta ttaccgagat
     9481 tccttttggc aaaaccacct cgggcattat agactccatt ttaaaagcca acgaaaaaaa
     9541 taaaatcaag atccggaaag tagacgataa taccgctcaa aatgccgaga ttttgataca
     9601 cctggcttcg ggcacatctt cggataaaac cattgatgca ctgtatgctt tcagcgattg
     9661 cgaggtaagc atttctccca attgctgtgt gattgcagac gaaaagcctc aattcctgac
     9721 ggtaagcgat gtgttgaggt attcagccga caacaccaag gaattgctca aaaaggagtt
     9781 gcttatccgc aagggtgaat tgctcgaaag cctcttgttt gcgtccctcg agaagatttt
     9841 tatcgaagaa aggatctaca aggacaaagc tttcgaagaa agtaccagca tggacaaggc
     9901 cctgtcgcat atcgacaaaa ggctggagcc cttcaaaaag gattttgtac gcgaggtcaa
     9961 acgcgatgat ttgctgcgat tgacggaaat taaaatggcc cgtatcctga aattcaacat
    10021 cgacaaagcc gaagaagcca tagtagcgat gcaggaacag atcaaggaga tcgatcatca
    10081 cttggccacc ctggtcgaat acaccattgc ctggttcgaa agcctgaaaa agaaatacgg
    10141 tgccgctttc ccccgcaaaa ccgagatccg gagtttcgat accatcgaag cggccgaagt
    10201 agtggaagcc accgaaaaat tatacatcaa ccgcgaagaa ggttttattg gtacttccct
    10261 caaaaaagac gaattggttt gtccctgttc aaatttggac gatgtcattc ttttttacaa
    10321 agatggcaag tacaaggtgg tcaaggtaag cgacaagatg tttgtcggca aaaacgtgct
    10381 ttacctgaat gttttcaaga aaaacgatac ccgcaccatc tacaatgtca tttaccgcga
    10441 cggcaaattc gacaacaaag gaggaggcgt ttcctatatc aagcgttttg ccgtgagcgg
    10501 cgtaaccaga gataaggaat acgatctgac caaaggcacc ccgggttcgc gtattcagta
    10561 tttcagcgcc aatccgaatg gcgaggccga aattgtccgt gttaccctca agccggctcc
    10621 cagaatcagg aaattgcaat tcgaaaaaga ttttggagag atagccatca aaggccgtca
    10681 atccatggga aatattttga ccaaaaacga tgtcttcaaa attgcgctga agcaaaaggg
    10741 cggctccaca ttgggcggcc gcgaagtgtg gttcgatccc gatgtgctga gactgaatta
    10801 cgacggacgc ggcgaatacc tgggtgagtt ccacgccgaa gacagcattt tggttatgac
    10861 aaaaagcgga gaatactata tgacgaatat tgacctgagt aatcattacg aggacaatat
    10921 tttgcgcata gaaaagtttg aagaggacaa aatctggacg gcggcgcttt ttgatgcaga
    10981 gcaacagaac tatccttatc tgaaacgttt cccgctcgag ttttcgctac gcaaactgag
    11041 ttttaccgga gagaatcccg aatcccggct aatactgctc agcgatactt attacccgcg
    11101 catcgaagtc agctttggag gcgacgacag tcatcgggaa aaactggtac tggattgcga
    11161 tgaattcata ggaaccaaga gtttcaaagc caaagggaaa cgcataacca cctacgaagt
    11221 tgccgaaatc aaggaaatag agcctttacg ttttccgcct cccaaagaag acgacaacaa
    11281 ttccgaacca gaagccgtaa ttgtttctga gtccgatgaa tctgaagagg acaacaatcc
    11341 cggcaagctg atcgacgaaa ttaccggaca aatgactctt tttgactaat aaaattattc
    11401 agctaccttt gcgctgattt ttaggcggct gtggtggaat tggcagtcac gtcagactta
    11461 ggatctgatg ccgaaaggcg tgggggttcg agtcccttca gccgcacttt ttacaattta
    11521 ggagaaaacc gaggcggttt tctcctaaat ttgttttggg gcaatctttc tttctgttgc
    11581 tttctccggt aaaatccagg tgttgatgcc ttcttcaaaa acaaaccgaa acccatcttg
    11641 ttgtacaaga accgttaaag tgtgcggttg gtgccttctt caaaaacagg ccgaaactca
    11701 ttcgaaatgg agtagaattt tgcgataaaa ataaaaagtg tttacttcac agcctaattg
    11761 attatgaata caataaaagg aatatcagga aaaataccgg gcctaacctt tgggcttgta
    11821 tattctttag ccctagttgg ttgcaaacac agtataggag ctgacaatca tttcgaccac
    11881 acgggggcaa gcaacgacag catagatatt taccctcaat tgcacagcag ctgcaccaag
    11941 taaattcctt ttctataaaa caacgagtaa taagcgataa aatatatgtt tatgaaacca
    12001 acctgcagaa ctctcttctt tactctcctt ttgagcataa tggcgtccgg ctccgtgtgt
    12061 tccggcaatt ggaaccattt taataacaac tggaaattca ccaaaggcaa tcccgaaaat
    12121 gcccaatcga caaactttga tgattcttcg tgggaaaagg ttaatctgcc ccacgactgg
    12181 gctataaagg gcccttttaa cgaacaggga aacggtagta ccgggaaatt gccctggaga
    12241 ggcgaaggct ggtatcgcaa cacatttact ctaagttccg aagaccaggg caaagtggtg
    12301 tatataaaat tcgacggagt tatgtcgtct ccgcagatat ttgtgaacgg aaagcttgcc
    12361 gggaaatggg attatggcta cagcagcttt tacctcgata tttcagacta tgtaaaatac
    12421 ggacaggaaa acagcgttgc cgtgtatgtt gacaacagga agcacgatag tcgctggtat
    12481 cccggagccg gcatttaccg taaggtggaa atgatgacaa ccgaaaaagt acatgaagaa
    12541 atctggggga cgtatatcac aacacctgtt gtaagccaat cttatgcgga aatgcgtatt
    12601 ttgtcgaatg tctggaacaa ggattccgaa gacaaaaaag ttacggtaga agccgtgctt
    12661 gtcagtcccg aaggatttga aattaaaacc gtcgcttcga gacaaagaaa cctgcccgcc
    12721 ggaagtcttt tccaatttga caagtggatg actgttgtcc ggccgcaaat ctgggatgtg
    12781 gaccatccgg ttttgtacag cctaaaaacc aaggtaatag tcgatggtaa agtggtggat
    12841 tccaacgaaa cgccttttgg tttcagaagc tttgaattta ctgccgacga tggttttcat
    12901 ctcaatggaa gacgattgca actcaggggc gtaaaccttc accatgatca cggccccttg
    12961 ggagctgctt ttaataaacg ttccatggaa cgtaaactgg aaatcctgaa agagatgggt
    13021 tgtaatgctg ttcgcaccag tcataatatt tgcgctcccg agttgattga attatgcgac
    13081 aaaatgggac tgctggtttt taacgaagcc ttcgataaat acgacaaaaa agcggatatt
    13141 tttgaggcta ccgattttta tgaatttgga gaacgggtaa tcaagaattt cgtgctgcgc
    13201 gataggaata atccttcggt gtttatttgg agtgtcggaa atgaaatggg cgatatacag
    13261 agcaattcca attttggctt acagaggttg gccgctatgg tgggctttgt caggaaatac
    13321 gatattacgc gcccggtaac catggcttgc gaccaggaaa gcaacgcaca ttggcgtcat
    13381 ttcgattact acgatgttca tgcctggaat tacggtcagc gctggaaaac agcccgaaaa
    13441 cttgatcctt ccaaatcggt aatcataagt gagtcggcat caactgtcag cacacggggc
    13501 ttttatgagc tgcctcttcc cgaaaaaccg accgatttca cccaatcaaa ccaggtgagt
    13561 tcttacgatt tacacgcacc ctcctgggcc gaagtctgcg acgatgattt tatgtggcag
    13621 gaaaacgata aatacattgc cggtgaattc gtatggaccg gtttcgatta tttgggcgaa
    13681 cccactcctt attcaagctc acgcagttcc tattttggta ttgtcgattt gtgcggtatt
    13741 cccaaagacc ggtattacct gtaccaaagc tattggcggc ccgaaaaaga cctgatacat
    13801 attttgccgc actggaattg gccaggacat gagggtaaaa aagtgcccgt atttgtttat
    13861 accaatggcg acgaagcgga attattcctg aacgggaaat cgttgggtaa acaatcaagg
    13921 aaacccaaat cggagatttc caccgaaaga tatcgtttga tgtggcacga tgtggtttac
    13981 gaagccggcg aattgaaagt ggtggcttac cgggtgggga aagtgattgg agaaaaagtg
    14041 ttgcgtacgg ccggcgaagc ctatacatta aaactcaccc ccgatagaag ccggctgaaa
    14101 gcaagcggcg aggatttaag ttttattctg gtcgaagctt acgataaaaa cggaaatctt
    14161 tgtccgctgg ccga
//
LOCUS       DAOSTD010000043        13933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_4465_length_13933_cov_14.456622, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000043 DAOSTD010000000
VERSION     DAOSTD010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13933
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4465_length_13933_cov_14.456622"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLI37_04345"
     CDS             complement(<1..451)
                     /locus_tag="PLI37_04345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ58275.1"
                     /translation="MKLSVIIVNYNVRYFLEQCLHSLYRAIKNTETEVFVVDNCSMDD
                     SLEMLRQSYPEIKLIANSENLGFAKANNLAIEASESDYVLLLNPDTLVQEEAIRNMLS
                     FMDAHPEAGALGVKMIDGRGRFLAESKRGLPTPAVSFYKISGLSAMFP"
     gene            complement(459..2555)
                     /gene="recG"
                     /locus_tag="PLI37_04350"
     CDS             complement(459..2555)
                     /gene="recG"
                     /locus_tag="PLI37_04350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOQ58276.1"
                     /translation="MKLSSLELTYLRGVGTARAELLKKELQIFNQEQMLYYFPYKYID
                     RSRIYKISELNANMPYVQVSGRIISFKIIGEGRAKRLSAGFTDGSGLLELVWFKGLKY
                     TQDRYKLNQEYLVFGKPSQYGTRLSIAHPDIDPLNEQGLAFTRGLQAFYNTTERMKSS
                     FMNSKAIQKIQISLFQSLTAPIPETLPGFLLEKLRLMSLDKSLREVHFPSDNKSLEKA
                     RYRLKFEELFYLQLHILRYARKRDKKFKGYPFTRIGDYFNTFYHKYLPFELTDAQKRV
                     LKEIRINVGGQSQMNRLLQGDVGSGKTLVALMSMLMAADNGYQACMMAPTEILANQHY
                     ATLCGLLGDMPLKVALLTGSTRKKEREILHRELQSGEIHFLVGTHALIEDTVQFANLG
                     LVIIDEQHRFGVVQRSKLWTKNTMPPHVLVMTATPIPRTLAMTVYGDLEVSVIDELPP
                     GRKPIQTLHRYDNKLAALYAFMRKELKAGRQIYVVYPLIEESEKMDYKNLQDGYQHIC
                     EAFPGIEVGMVHGKMKSADKDEQMLRFVKGETRILVATTVIEVGVDVSNASMMLIQNA
                     ERFGLSQLHQLRGRVGRGADQSYCVLLTSYKLSDEGRKRIEIMVSSNDGFEIAEADLK
                     MRGPGDLEGLQQSGVPFDLKIADLGKDVQILEYARQIAGECLEKDPDLSLPENALMAE
                     ELKKRYGKKINWGAIS"
     gene            complement(2561..3244)
                     /locus_tag="PLI37_04355"
     CDS             complement(2561..3244)
                     /locus_tag="PLI37_04355"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOQ58277.1"
                     /translation="MKAYALIMAGGAGQRMAAEIPKQFLLLHGRPILMHTLSAFYQYS
                     PAVKLVLVIPRQQQELWQKLCIQYSFDLPHLLAYGGKERFDSVRNGLQKIAGSEGFDA
                     ENSLIAVHDAVRPLVGVHLIETCYQTAERYGSAIPVTPLVDSLRRRDGEWSLAENRLD
                     FCAVQTPQVFNAKALLEAYRQPYHPSFTDDASVMEAAGHRIRLVEGHRKNSKITVPED
                     LLLAEYWLK"
     gene            complement(3246..4310)
                     /locus_tag="PLI37_04360"
     CDS             complement(3246..4310)
                     /locus_tag="PLI37_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOQ58278.1"
                     /translation="MKKLLLILTLSLALFSLAAKPVKNVIFLIGDGMGLNQVYAAALV
                     KGAPLYMMQDTKTGLILTYSYDNGITDSAAGGTALAAGVKTRNSMIGMGPDSVKVESI
                     LHQAARNGKSTGVMSTSAITHATPASYVANQVSREMQDEIALDFVKQGPDVFIGGGRR
                     YFEEREDGVDLTEQLKSKGYEVVYDLEHAEASGSAKLAAFLADDHLPDVISGREYLLP
                     EALNIALERLSRNKKGFFLMVEGSKIDMRCHVHDIMGSVAETLEFDETVKVALDFARK
                     NKNTLVIVTADHETGGLSVLQNAPYRKGEELIVHFSTGGHSGVPVPVYSYGPGSDNFT
                     GVLENTYFKATLARLMKLKP"
     gene            complement(4415..4615)
                     /gene="xseB"
                     /locus_tag="PLI37_04365"
     CDS             complement(4415..4615)
                     /gene="xseB"
                     /locus_tag="PLI37_04365"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOQ58279.1"
                     /translation="MKSPISYQKAIEELESILQFLEQNEADVDQMTQKLKRATELIGF
                     CREKLHKTDLELQAIFKELEEK"
     gene            complement(4626..5978)
                     /gene="xseA"
                     /locus_tag="PLI37_04370"
     CDS             complement(4626..5978)
                     /gene="xseA"
                     /locus_tag="PLI37_04370"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOQ58280.1"
                     /translation="MKHNFSDFTQAETGRAQPKTLYELNNMLRRGIRDLFPENLWVQA
                     EIAECKENASGHCYLELVEKQGDSDYLRARAKAVIWSNVWIFLREAFTQQTGSQLQAG
                     QKILILVKPDFHELYGLSLVVLDIDPAYTVGEWALRRRAVLSRLEQEGVLELNKELDW
                     PRLPQRLAVISSPTAAGLEDFRHQLQHNSGGFVIYTCLFPALMQGAQAESSILEALDR
                     VLEHQELFDLVVLIRGGGATVDLSCFDGYELASALAQFPLPLLSGIGHERDRSVADMV
                     AHTSVKTPTAAAELILSCLEQEDRKIEDMAASLLAMGQETISAGVRQLQFYKERIVQL
                     SSVQKNRGQLQIQKIESELRWAARERLQQRRHCLDAAVQTIGLLSPQILARQLQRLDV
                     LKLKLEAYSPELQLKRGFSITLHQGRILHSAEGLPPGSLLETRLEDGRVFSKTIQKQT
                     "
     gene            6121..6537
                     /locus_tag="PLI37_04375"
     CDS             6121..6537
                     /locus_tag="PLI37_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOQ58281.1"
                     /translation="MKSYRKELSFQTETRRAYINITPQIESCLKESGIKEGLLLCNAM
                     HITASVFINDDEAGLHADFEQWLEKLAPEKPHSQYRHNGYEDNADAHMKRQVMGREVV
                     VAVTGGKLDFGTWEQIFYGEFDGKRRKRVLVKIIGE"
     gene            complement(6544..8730)
                     /locus_tag="PLI37_04380"
     CDS             complement(6544..8730)
                     /locus_tag="PLI37_04380"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOQ58282.1"
                     /translation="MKTRTIILTLAAALLLTACVQTNPDAQADPGVQTGLSSQSPDVG
                     NPLLPGYFADPTIVEFDGIYYIYATTDNEMLASGAPTLWYSRDLLNWYNYTLDIPSLE
                     SINLRNFWAPDIVKGDDGRYYLYFGNCQAGCNIYGYVSSTPVGPWTKLHQDDTPVIGS
                     GFPRRGFPSLDAQFFRDDDGKIYAYWGTWVHYNGGYAVGELDSKSMSRMINGKNIPLE
                     QTPEPFEAAYVLKHKDKYILMYSSASCHDASYNVRYSYADNPYGPFTPGDNNPILSTN
                     ADQSVHGPGHHSVLKQGEDYYIVYHRHDYPMTRGGMARQVCIDSMIFENDSTIRKVIP
                     SHRGIADIFPSELPVDLAFGAKTKASSYYRMNSPGYDYTYKPSFATDNNNATMWKAAD
                     NSFPQTLEIDLGSVQKISRVMTQFEFASYYYQYLLEYSTNGKKWAVYADRSDNRVSGS
                     PMIDDKEVEARFLRLKVLGAEKTGIYAAVWNIKAYGSSFDIPLGLVNKASEAGPGAAS
                     KREMLLGFEIGKKSKAENLSSLPNLGTLGGNFTLRGEVSLGTDDLGVQALEFTRGSLV
                     LDVPVPQRLAWNGSFTVATWVRNPEVGREGDCLFSWCDRHALRLANSYNALFYNRGPY
                     GAMAHLDGHFDMRYRNLPAENEWHHIVVTFDGVLEKVYVDGVLDNVQSMILASAVDRA
                     RFIVGASDVGEHFSGYMASLRMYDYALSPKELSKLMKKTRPENLKK"
     gene            9147..12506
                     /locus_tag="PLI37_04385"
     CDS             9147..12506
                     /locus_tag="PLI37_04385"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOQ58283.1"
                     /translation="MLLLFALLFTALPDIRGQIRFEQGSSFRYLKGNANHQSPETLFS
                     RDYDDSQWSTGPAPFRYGKGSGGTVLSDMQNNYTCFYLRCTFRAENVDRLEQLHLSIN
                     YDDGFVIAINGQIVLKQNVPETLSNNSLALQNHDPGTNESFVLNAMDIPLVEGDNSLA
                     VMVLNVSSHSSDIHFDLGMTAEPALPAFPYSGNVQISHKAGFYDEAFTLTLESPYPDC
                     NILFSFDGSEPATSSTAINAGTWREWPIDPTQPILRGQTPAYVLRACLVKEGYKPSVS
                     ITRTYIFTDQVKTQTHPGWEWPVGRHNGQRLDYEMDTAVVNHKNYSPYIDAALRQIPS
                     ISIVTDMKHLFDAGSGIYVNAEKSGRAWERPCSIELIDTCETKGFQINAGIRIRGAAS
                     AKQKESVKRAFRFFFRKEYGASKLKYPLFDNEGVEEFDRMDLRCEQNYSWSKDGSKYN
                     SMIADIFSRDLQGRMKQPYKRGRYYHLYLNGMYWGLYQTDERAEANNAEHYFGGHKDD
                     YDVIKVYSEFWPYTNEATDGNLEAWTALWNKCTEGFASNEAYLALLGKKPDGSMDPEG
                     RVWVDIDNLIDYMLVIFYTGNFDAPTSAFGNDDMPNNYYAVYNRNDHSRGFRFFAHDS
                     EHSMFVDNVFVGGLNCNRVNIGTNGSMQINRMLEFNPQWLHYKLCSNEEYRFRFMDRA
                     YKYLSVGGLLRPDSAAALYRSRAAQIDLAVIAESARWGDAVSHTTLGKKDWETLLQSM
                     YNEYFPKRTNILIQQLKAEGLYTTIAAPGISINNYRFYGSDYSFRGEVPVSISNLSGP
                     SSSLYYTLDGSDPRTFGGGISSKAINVRGSVSFSINQSTVLSCRIYSGTNWGPLKQVF
                     FSNADEDYQALKITEIHYHPKDTFVNSVLTKGQDFEFIEFKNTGNKAIDLSGLKIDSG
                     IYHRFPEQQLLYPGEFYVAASKPNNFYLRYGRYPSGSFSRNLSNSGEKIVLLDPANQP
                     LISFDYYDKSPWPTEPDGSGYSLAAAQAMPEGDPGDYAYWMTSSHMGGSPFADDPGSA
                     SANDSPGKFLAARLDVYPNPSKGTFFVNTGQNESYRLRIYDSKANLIFDRQYEGIAAI
                     DFGTENLSRGLYILRAELPGEVLSTKLIYLP"
     gene            12659..13690
                     /locus_tag="PLI37_04390"
     CDS             12659..13690
                     /locus_tag="PLI37_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOQ58284.1"
                     /translation="MKHHLFSTSWILRLTGLLLGVLLIQNGYGQERQQQARQVRRVPI
                     DSIRLSDPCILADKASGLYYMTGTGGLLWKSTDLHIWEGPYVVAQTDPESWMGPRPMI
                     WAAELHRYKGKYYYFATFTNRSVKIDTVAGNVIERRASHVLVSDKPEGPYVPMADPTY
                     LPADKPTLDGTFWVDTDGKPYMVFCHEWLQNWNGTIEKIELKPDLSGSVGESTILFRA
                     KDSPWSRERDEQGNVGPNKVTDGPYLFRTGSGRLGMIWTSWIYDVYAQGVAYSESGNL
                     DGPWIQEPELITPPNFGHGMLFETLDGKTLMSVHSHQNINGRYHRVPHLFEVDLSGDK
                     LVLGKEVKF"
BASE COUNT         3648 a         3574 c         3254 g         3457 t
ORIGIN      
        1 ggggaaacat cgcagacaaa ccggaaatct tataaaaaga aaccgccggc gttggcaaac
       61 ctcttttgga ttccgccaaa aaacggcctc gtccgtcaat catcttgact cccagagcgc
      121 cggcctccgg atgtgcatcc ataaaggaca gcatattacg aatggcctct tcctgcacca
      181 aggtatcggg atttagcaaa agcacataat cggattcgga ggcttcgatg gccagattgt
      241 tggctttggc aaaacccagg ttttcgctgt tggcaatcaa ctttatttcg ggatacgact
      301 gccggagcat ttccagcgaa tcgtccatgg agcagttatc aacaacaaaa acttctgttt
      361 ctgtgttttt tattgctcta tacaaagaat gcaggcattg ttccaaaaaa tacctgacgt
      421 tgtagtttac tatgattacc gataacttca cttcattact aactgatagc tccccaattt
      481 attttcttgc cgtagcgttt ttttaattcc tcggccatca aagcattttc gggcaaagat
      541 aagtccggat ctttctcgag gcattccccg gcgatttggc gggcatattc caatatctgg
      601 acatctttgc ccagatcggc aattttcagg tcgaagggaa ccccactttg ctgtaagcct
      661 tccaagtcgc ccggcccacg cattttcagg tcagcttcgg ctatctcgaa accgtcgttg
      721 ctcgaaacca taatctctat gcgtttacgc ccctcgtcag agagcttgta tgaagtcaac
      781 aacacacaat aggactgatc ggccccgcgt cccaccctgc ctctcaactg gtgcaattgc
      841 gacaaaccga aacgttcggc attctgtatg agcatcattg aagcattaga cacgtccact
      901 cccacttcga taaccgtagt ggccaccaaa atccgggtct ccccttttac aaaacgaagc
      961 atttgttcgt ctttgtcggc tgatttcatt tttccgtgaa ccatgccgac ttcaattccg
     1021 ggaaatgctt cacaaatatg ctgataaccg tcttgcaggt ttttataatc cattttttcg
     1081 gactcctcga tcaggggata gaccacgtag atttgtctgc ccgccttcaa ctccttgcgc
     1141 ataaaagcat atagggctgc cagcttgttg tcataacgat gcagggtttg aatgggtttg
     1201 cgtcccgggg gcaattcatc tattaccgag acttcgaggt ctccatagac cgtcatggcc
     1261 aaggtccggg gaatgggtgt ggccgtcatc accaacacat gcggcggcat agtgttttta
     1321 gtccagagtt tcgaacgctg aaccacgccg aaacgatgtt gttcgtcaat aatcaccagg
     1381 cctaagttgg caaactgcac cgtgtcttct atcaacgcat gagtaccgac cagaaaatga
     1441 atttctccac tctgtaattc ccgatgcagg atttcgcgtt cttttttccg ggtggagcct
     1501 gtcagcaagg ccactttgag gggcatatct ccaagcagcc cgcacaaagt ggcgtaatgc
     1561 tgattggcta gaatttcggt gggtgccatc atgcaagcct gatagccatt gtcggctgcc
     1621 atcagcatag acatcagggc aacaagggtt ttaccactac ccacatctcc ctgtagcaga
     1681 cgattcattt gcgactgccc gcccacgttg atacgtattt ccttgagcac tcttttttgc
     1741 gcatccgtaa gctcaaaagg cagatacttg tgataaaaag tattgaagta atcgccgata
     1801 cgagtaaaag gatagccctt aaactttttg tctctcttgc gggcataacg caaaatatga
     1861 agctgcaaat aaaaaagttc ctcgaatttc agcctgtacc tggctttttc gagcgactta
     1921 ttgtcgctgg gaaaatgcac ctcgcgcaaa gacttgtcca gagacattaa acgcagtttc
     1981 tcgagcaaaa agccgggcaa ggtttcggga atgggggctg tgaggctttg aaatagggaa
     2041 atctgaatct tttgaatcgc tttagaattc ataaaactcg atttcatcct ttccgttgtg
     2101 ttgtaaaaag cctgcaatcc ccgggtaaaa gccaggccct gctcgttgag cggatctatg
     2161 tccgggtggg caatcgacag acgggtaccg tattgcgagg gcttaccaaa cactaagtat
     2221 tcctgattga gtttgtacct gtcctgggtg tacttcaaac ccttgaacca caccaattcg
     2281 agcaaaccgc tgccgtcggt gaaaccggcc gataaacgtt ttgccctgcc ttcgcctata
     2341 attttgaacg aaataatacg cccactgacc tgcacatagg gcatgttagc gtttaactcc
     2401 gagattttgt atatgcggct acgatcgatg tatttatacg ggaaataata cagcatctgc
     2461 tcctgattga aaatctgcaa ttcctttttc aggagctcag cccgcgcagt acctactccg
     2521 cgcagatagg tcagttccag agacgaaagc ttcacttcaa ttactttaac caatattcgg
     2581 ccagaagcag gtcttcgggc accgttatct tactgttttt acgatgacct tccaccaggc
     2641 gaatacggtg gccggcagct tccattaccg aagcatcgtc ggtaaaagaa gggtggtacg
     2701 gctgcctgta agcctccaaa agagctttgg cattgaaaac ttgaggtgtt tgcacggcac
     2761 aaaaatccag tcggttttcg gccaggctcc attcgccgtc cctgcgccga aggctgtcta
     2821 ccaaaggagt aacaggaata gccgaaccgt aacgctcagc cgtctgataa caggtctcga
     2881 taaggtgaac ccccaccaaa ggtctgacgg catcgtgaac ggcaataagg ctattttccg
     2941 catcgaaacc ttccgatccg gctatctttt gcaagccgtt tcgaacggaa tcaaatcgtt
     3001 cttttcctcc ataggccaaa agatgaggca aatcaaaaga atattgtatg cagagttttt
     3061 gccacaactc ttgttgttgc cggggaatga ccaacaccaa ttttacggca ggactgtact
     3121 gataaaaggc gctgagtgta tgcatcaaaa tgggccgtcc atgcagcagt aaaaattgct
     3181 tgggtatttc tgcagccata cgctgtcccg ccccaccggc cattattaat gcataagctt
     3241 tcattttacg gtttcaattt catcagccga gccaaggtag ctttaaaata agtattctcc
     3301 aaaacgcctg taaaattgtc cgagccgggg ccataggaat aaacaggtac ggggactccg
     3361 gagtggccgc cggttgagaa atgaactatc agctcttcac cttttcggta aggtgcattt
     3421 tgtaaaacac tcaagccacc ggtttcatga tcggcagtaa caataaccag cgtgttttta
     3481 tttttgcggg cgaaatcgag ggctactttg accgtctcgt caaattccag agtttcggca
     3541 acgctaccca taatgtcgtg tacatggcag cgcatatcaa tcttggatcc ctctaccatc
     3601 aaaaagaaac ccttcttgtt ccggctcaaa cgctccaggg ctatgtttag agcctcgggc
     3661 aacaaatatt cgcgtcccga aatcacatcg ggcagatgat catcggccaa gaaggccgcc
     3721 agtttggcgg agccggaagc ttcggcatgt tccagatcgt acaccacttc ataacccttg
     3781 gatttgagtt gttccgtcag atcaactccg tcttcacgtt cttcgaaata cctgcgtcct
     3841 ccgccgataa agacatccgg gccttgtttt acaaaatcca gggcaatttc gtcctgcatt
     3901 tcgcgcgata cctgattggc cacataagaa gccggggtag catgggttat agcggaggta
     3961 gacatgacac cggttgactt gccattgcgg gcagcctggt gcagaatgga ttccaccttc
     4021 acactgtcgg gccccattcc tatcatagag ttacgggttt tcactcctgc tgcaagagcc
     4081 gtaccgccgg cagccgaatc ggtaataccg ttatcgtagg aataagtcag gataagacca
     4141 gttttggtgt cctgcatcat gtacagagga gctcctttaa ccagagccgc agcataaacc
     4201 tgattaaggc ccattccatc gcctatcaaa aaaatgacgt tcttaaccgg tttggcggca
     4261 agactaaaca gcgcgaggct cagagtcaat atcaacaata actttttcat cgtggttttt
     4321 ttatggattt attttatacc ggcaaatgta cttataaaag aaccaagctt tgctttaagt
     4381 agtgttaatc agacctaaaa caataaattc cggactactt ttcttccagt tccttaaaaa
     4441 tggcctgcaa ctcgaggtcg gttttgtgca acttctcgcg acaaaaacca atcaattcgg
     4501 tggccctttt taatttttga gtcatttgat ccacatcggc ttcgttctgt tccagaaatt
     4561 gtaaaatgct ttccagttct tcgatggctt tttggtaaga aataggcgat ttcatcttga
     4621 aaaaattagg tctgtttttg tatggtcttg gaaaaaacac ggccgtcttc gaggcgggtt
     4681 tcgagcaggc tacccggagg aagtccctcg gccgaatgca aaatcctgcc ctggtgcaaa
     4741 gtgatcgaaa acccacgttt taactgcaat tcgggagaat aagcttccaa ttttaatttc
     4801 agcacatcga gtcgttgcaa ctgacgtgca agtatttggg gagacaagag gccgatggtt
     4861 tgcactgccg catccaagca gtgtcttctc tgttgaagac gttctcgtgc ggcccatcgt
     4921 aattcggatt cgattttttg gatttgcagc tgcccccggt ttttttgaac agacgacaac
     4981 tgcacgatgc gttccttgta aaactgcaat tgccggacac ctgcgcttat ggtttcttgc
     5041 cccatagcaa gcaggcttgc agccatatct tctatcttcc ggtcttcttg ttccaggcaa
     5101 gacagtatca gctcggcagc tgccgtaggg gtcttgaccg aagtgtgcgc caccatatcc
     5161 gccaccgagc gatcgcgttc atgccctatg cccgacagca aaggcaaagg aaactgagcc
     5221 agggccgaag ccagttcgta gccgtcgaaa caggaaagat cgaccgttgc tccccctccc
     5281 cgtatcaaca cgaccaaatc gaaaagttcc tgatgttcca acaccctgtc cagggcttcc
     5341 aatatggaag attctgcctg cgcgccctgc atgagggcag ggaaaagaca ggtataaatc
     5401 acgaagcccc cggaattatg ttgtaattga tgcctgaaat cttcaaggcc ggcagccgtg
     5461 ggcgatgaaa tcaccgccag gcgctgaggc aaacgaggcc agtccagctc cttattgagt
     5521 tccagaacac cttcctgttc cagtcgcgat aaaacagccc ggcgacgcaa ggcccattcg
     5581 cccacagtat aagcgggatc aatatcgagc accaccagac tcagaccgta taattcatga
     5641 aaatcgggct taaccagaat caggattttt tgtccggcct gaagttggct gccggtttgt
     5701 tgggtaaatg cctcgcgcaa gaaaatccat acattcgacc aaataaccgc tttggctctg
     5761 gcacgtaaat agtcggaatc cccctgcttc tcgaccaatt ccagataaca atggcccgaa
     5821 gcattttctt tgcattcggc aatctcggcc tgtacccaga ggttttcggg aaagagatcc
     5881 cgaatccccc tgcgcaacat gttgttaagc tcatagagag ttttaggttg agctctgcct
     5941 gtctcggctt gcgtaaagtc ggaaaaattg tgcttcatcg cttcggtagg gtttttgtcg
     6001 ccaagattgt tttcgtagtt ttgatagctt gaagagggca aatgtaatga ataatttgta
     6061 tgtttttgtt tggaattatt ggagtcaatc gatacttttg agcctttaag caagttttaa
     6121 atgaaatcat accgcaagga acttagcttc cagaccgaaa cacgcagagc ttacataaat
     6181 attactcctc aaattgaaag ttgcctcaaa gagtccggca taaaagaggg gctgttgctt
     6241 tgtaatgcca tgcatatcac cgccagtgtt tttatcaacg acgacgaggc gggcctgcat
     6301 gccgactttg agcaatggct cgagaagctg gcgcccgaaa aacctcacag ccagtaccgt
     6361 cacaatggat acgaagacaa tgccgatgcc catatgaaac gccaggttat ggggcgcgaa
     6421 gtggtcgtgg ccgtcaccgg gggcaaactg gatttcggga cttgggagca aatcttttac
     6481 ggagaattcg atgggaaaag acgcaaacgc gtgctggtaa aaattatagg ggaataaacc
     6541 acattatttc ttcaaattct caggcctggt cttcttcatc aatttgctga gttccttcgg
     6601 gctgagcgcg taatcgtaca tacgcagcga agccatataa ccgctgaaat gttctcccac
     6661 atctgaagct cccactataa agcgggctct atccactgcc gaagccaaga tcatgctctg
     6721 aacgttgtcg aggactccgt ctacatatac tttttccaac acaccgtcga aagtaaccac
     6781 aatatgatgc cattcgtttt cggccggcaa attgcggtaa cgcatatcga aatggccgtc
     6841 cagatgggcc atagccccgt atgggccgcg attgtaaaac aaagcattgt aggaattagc
     6901 caggcgcagg gcgtggcgat cgcaccagga gaacaggcag tcgccttcgc gcccaacctc
     6961 gggattcctt acccaggtag ccaccgtaaa cgagccgttc cacgcaagcc tctgaggcac
     7021 aggcacatcc aagactaaag atccacgggt aaattcaaga gcttgcacgc ccaagtcgtc
     7081 cgtgccaagc gacacttcgc ccctgagtgt aaagtttccg cccagcgtac ccaggttggg
     7141 caggctgctt agattctctg ccttactctt tttgccgatt tcaaagccga gcaacatttc
     7201 ccgcttgctt gcggctcccg ggcctgcttc ggatgccttg ttgaccagac ccaggggaat
     7261 gtcgaaggag gagccgtagg ccttaatgtt ccaaacagca gcatagatac cggttttttc
     7321 ggcccccaat acttttagtc tcaaaaagcg ggcttccact tccttgtcgt cgatcatggg
     7381 gctgcccgaa acccggttgt cggaacgatc ggcgtaaaca gcccattttt tgccgttggt
     7441 agaatattcc aacagatact gataataata cgaggcaaac tcaaactgag tcattactct
     7501 gctgattttc tgcacactcc ccaaatcaat ctccagggtc tggggaaaag aattatcggc
     7561 cgccttccac atggtggcgt tgttgttgtc ggttgcaaac gaaggcttgt acgtatagtc
     7621 ataacccgga ctgttcatgc ggtaatacga agaggccttg gttttggctc caaaggccag
     7681 atcgaccggc aactccgaag gaaaaatatc ggctatgccc cgatgcgaag gaattacttt
     7741 acgaatggtg gaatcgttct caaaaatcat gctgtcgatg caaacctgcc gcgccatacc
     7801 gccccgggtc atgggataat cgtggcgatg atatacgata taataatctt cgccctgctt
     7861 caacaccgaa tgatgtccgg ggccgtgtac cgactggtcg gcattggtgc tgagaatggg
     7921 attgttgtca ccgggagtaa aaggaccata gggattgtcg gcataggaat agcgcacgtt
     7981 gtaactggcg tcgtggcagg aagcgctgga atacatcagg atatatttgt ccttatgctt
     8041 gagcacgtaa gctgcctcaa aaggttccgg tgtctgctcc aggggaatat tttttccgtt
     8101 gatcatgcgg gacatgctct tactatcgag ttctcccacg gcatagccgc cgttgtagtg
     8161 cacccaggtg ccccagtagg cgtagatttt tccatcgtcg tccctgaaaa actgagcatc
     8221 cagcgaagga aagcccctgc ggggaaagcc gctgccgatt accggagtgt cgtcctgatg
     8281 caatttagtc cagggtccca ccggcgtgga agaaacatag ccgtaaatat tacatcctgc
     8341 ctgacaatta ccaaaataca ggtaatagcg cccgtcgtcg cctttcacaa tatcgggagc
     8401 ccaaaaattc cgcaaattga tagattccag cgagggtata tccaaagtat aattatacca
     8461 attcaacaga tcgcgactat accacagcgt tggcgccccc gatgccaaca tctcgttgtc
     8521 ggtggtggca taaatgtaat aaataccgtc aaactccacg atggtgggat cggcaaaata
     8581 accgggcagc aaaggattac ccacatcggg cgactgggaa ctcagtcctg tttgtacacc
     8641 cggatcggct tgcgcatccg gattggtttg tacacaagca gtcaacagca aggctgccgc
     8701 caatgtcaaa atgattgttc tcgttttcat attaatttgg cttgaaatat aaaagctgga
     8761 aggatagtca aagataaacc tttttattta gcccttgaac acacaggagg gaataagtaa
     8821 aataattgca ctgaacggga taatagtatt attttaagca ccatacctac tagataatgt
     8881 tcaaatcccg atcacataat ccgatggtga ttaaaggggt ttaagcaagg agcttgcggc
     8941 tgacaaaatc gtctcaaatg catcaagaat tgtgtaatac agcaagatcc ggcaacaaaa
     9001 tcctcagaaa tatgtatttt tacgactgta tgtcaacaat cttagtcata aaaaaccttg
     9061 cccccggcag caacacccgt gcaagcaaaa gtctcagccc tggtccaaac ttccttgtcc
     9121 ccggctgcaa gaacttgtcc aaggttatgc tattattgtt tgcgctgctg tttacggccc
     9181 tccccgatat tcgggggcaa attcgctttg agcaaggcag cagttttcgc tacctcaaag
     9241 gcaatgccaa tcaccagagc cccgaaaccc tattcagccg ggactacgac gacagccaat
     9301 ggagcaccgg ccctgctcct ttccgctacg gtaaaggcag cggtggcact gtactgagcg
     9361 acatgcaaaa caattatacc tgtttttacc tgcgctgcac tttccgggcc gagaatgtag
     9421 acagactgga acaactccat cttagcatca actacgacga cggttttgtt attgccatca
     9481 acggacagat tgtattgaaa caaaacgtcc ccgagacact atcgaacaac tctctcgcac
     9541 tgcaaaatca cgatccgggt actaacgaga gcttcgtact caacgcgatg gatatccctc
     9601 tggtcgaagg cgacaacagc ctggccgtta tggtgttgaa tgtgtcttcc catagttccg
     9661 acatccattt cgacttggga atgacggcag aacccgcatt gcccgctttc ccttacagcg
     9721 gcaacgtaca aatcagtcat aaggccggat tttacgacga agccttcact ctgaccctcg
     9781 aatcgcccta tcccgactgc aacatattgt tcagcttcga cggcagcgaa cccgccactt
     9841 cttctaccgc catcaatgcc ggcacgtgga gagaatggcc catcgacccc acacaaccca
     9901 ttttgagagg gcagacgccg gcttatgttt tgcgcgcctg cctcgtgaaa gagggttata
     9961 agccttcggt ttccattacc agaacctata tttttaccga tcaggtaaaa acccagactc
    10021 accccggctg ggaatggcct gtcggccggc acaacggaca aaggctcgac tacgaaatgg
    10081 atacggccgt ggtaaaccac aaaaattatt cgccctacat tgacgcagcc cttaggcaaa
    10141 tccccagcat cagcatagta accgacatga agcatctgtt cgatgccggc agcggcatct
    10201 atgtgaacgc cgaaaaaagc ggccgggcct gggaacgtcc ctgttccata gaattgatcg
    10261 acacctgcga aaccaaaggc tttcaaataa acgcgggtat tcgcatcagg ggagctgcca
    10321 gtgctaaaca aaaagagagc gtcaaaaggg cttttcgttt cttcttcaga aaagaatacg
    10381 gagcctccaa gctgaaatac ccccttttcg ataacgaggg agtagaagaa ttcgaccgca
    10441 tggacttgcg ctgcgaacaa aattactcct ggagcaaaga cggcagcaaa tacaattcca
    10501 tgatagccga tattttcagt cgcgacctgc aaggccgcat gaaacaaccc tacaaaagag
    10561 gacgctatta ccatctctac ctcaacggca tgtactgggg gctctaccag accgacgaac
    10621 gggccgaagc caacaatgcc gagcattatt tcggcggaca taaagacgac tacgacgtca
    10681 tcaaagtcta ttccgaattc tggccctata cgaacgaagc caccgacggc aacctggaag
    10741 cctggacagc tctgtggaat aaatgtaccg aaggctttgc ctccaacgaa gcctatctgg
    10801 ccttgttggg caaaaaaccc gacggaagca tggatccgga gggcagggtt tgggtggata
    10861 tcgataatct gatcgactat atgctggtta ttttctacac cggcaatttc gatgccccga
    10921 cctcggcctt cggcaacgat gatatgccca acaattacta cgccgtatat aaccgcaacg
    10981 accactcgag gggattccgc ttttttgcgc acgattcgga acacagtatg tttgttgata
    11041 atgtcttcgt aggtggattg aattgcaacc gtgtgaacat aggaacaaac ggcagtatgc
    11101 aaataaaccg tatgctcgag tttaatcctc agtggttgca ctacaaactt tgctccaacg
    11161 aagaataccg ctttcgtttt atggaccgtg cgtataagta tttatccgtt ggcggtctgt
    11221 taaggcccga ttctgccgca gccctctatc gctcaagagc cgctcaaata gacctggctg
    11281 ttattgccga gtcggcccgc tggggagacg ccgtgagtca tacaaccctg ggtaaaaaag
    11341 actgggaaac cttgcttcag tccatgtata acgagtattt ccccaaacgc accaacatcc
    11401 tgatacagca gctcaaagca gaaggcttgt ataccaccat agcggctccg ggcatcagca
    11461 tcaacaatta tcgcttctac ggttcggatt acagttttcg gggcgaagtg ccggttagca
    11521 tcagtaatct ttccggtcca agcagcagtc tttactacac gctcgacggc agcgacccca
    11581 gaactttcgg aggcggaatt tcgtccaagg ccatcaatgt cagaggcagt gtttcgttca
    11641 gcataaacca aagcaccgtg ctgtcgtgca gaatttactc gggaaccaac tggggccctc
    11701 tgaagcaagt ctttttcagt aatgcagacg aagattacca ggccttaaag attaccgaaa
    11761 tacattacca ccccaaagac acttttgtaa attcggtatt gaccaagggg caagattttg
    11821 agtttatcga gttcaaaaac accggcaaca aagccataga tctgtccgga ctgaaaattg
    11881 actcgggcat ttatcaccgc tttcccgaac aacaattgct gtatccgggc gaattttatg
    11941 tagccgcctc caagcccaac aacttctact tgcgctatgg gcgctatccc tccggcagtt
    12001 tttcgagaaa cctttccaat tcgggcgaaa aaatcgtttt gctggatccc gctaatcaac
    12061 ccctgatcag tttcgattac tacgacaaaa gcccctggcc aacggagccc gacggatcgg
    12121 gctatagctt ggcagccgca caggcaatgc ccgaaggaga ccccggcgat tatgcctact
    12181 ggatgacctc cagccacatg ggcggatcgc cttttgccga cgatcccggt tctgcctctg
    12241 ccaacgacag tcccggcaaa ttcctcgccg cccgcctcga tgtctatccc aaccccagca
    12301 agggcacctt cttcgttaat accggacaaa acgaaagcta ccgcctgcgt atatacgaca
    12361 gcaaagccaa cctaatcttc gacagacagt acgaaggcat cgccgcaatt gattttggta
    12421 ccgaaaacct cagccgcggt ctttacatcc tccgcgccga attgcccggt gaggtgttga
    12481 gcactaagtt gatttatttg ccttgattgg aattttctct tcaacagacg gctgtgggtt
    12541 tagtattaaa aagaaccctg gcaacaatcc cggcaccaaa atagtgatat tcaggatcag
    12601 aatagcaaat ccaatttttc taaataatta attatatttg caattattcc tttttatcat
    12661 gaagcaccat ttattttcaa catcttggat tttaagactt accgggctgc tcttgggagt
    12721 tttgctgata caaaacggct atggacaaga gcggcagcaa caggcccggc aggtgagacg
    12781 ggttccgatc gattcgatac gattgagtga tccctgtatt ctggccgaca aagccagcgg
    12841 attgtattac atgaccggca ccggcggtct gctgtggaag agtaccgatc ttcatatctg
    12901 ggaaggtccc tatgtggtag cccaaaccga tccggaatcc tggatggggc cccggccgat
    12961 gatctgggct gccgagctcc atcggtacaa agggaaatac tattattttg ccacctttac
    13021 caatcgtagt gtcaaaatag acaccgtggc cggtaatgtg atcgaacgca gggcttcgca
    13081 tgttttggtg agcgacaaac ccgaagggcc ttacgtgccg atggctgatc ccacttacct
    13141 gccggcggat aaacctacgc tggacggcac tttttgggtg gataccgacg gtaagcctta
    13201 catggtgttt tgtcacgaat ggctgcaaaa ctggaacggt accatagaaa agattgaact
    13261 taaacccgac ctgagcggaa gcgtaggtga gtcaactatc ttgttcagag ccaaagattc
    13321 cccctggagt cgcgagcgcg acgagcaagg caacgttggc cccaacaaag taaccgacgg
    13381 accttatttg tttcgcacgg ggagcggcag gctgggaatg atctggacca gttggattta
    13441 cgatgtctat gctcagggcg tggcttattc ggaaagcggc aatctcgacg gaccctggat
    13501 tcaagaaccc gaattgatca ctccgccgaa cttcggtcac ggcatgctct tcgaaacctt
    13561 agacggcaaa acactgatgt cggtacacag tcatcaaaac atcaacggac gttaccaccg
    13621 cgtaccacat ttgttcgaag ttgacctgag cggcgataag ctggttctgg gcaaagaagt
    13681 gaaattctaa aacgaagtct gcttcaaagg ctcggattgg gtttgattta ttcggattgg
    13741 gttgattccg tttagtaacg tttctatttt gaattactaa ccggaaactc atagaagttc
    13801 atttcccctt gcaagatcat caactggtcc aactgaatgt aaggacttaa agcggtaatg
    13861 ctgatattta cccggccttt ttccatttcc cgggcaggga tttctattaa tgcctgtccg
    13921 cgtaggactt gtt
//
LOCUS       DAOSTD010000044        13910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_4476_length_13910_cov_13.028870, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000044 DAOSTD010000000
VERSION     DAOSTD010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13910
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4476_length_13910_cov_13.028870"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2606)
                     /locus_tag="PLI37_04395"
     CDS             complement(<1..2606)
                     /locus_tag="PLI37_04395"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOQ58285.1"
                     /translation="MRIKMFALLLLLLSPLVAFAAEYSIQSKMLDKATDGAIEMGSVR
                     LLTPGDSSLVAGTLSDQAGRFTLTKISAGKYILECRFLGYKTVYKDIEIKDRSITLPN
                     IFMEEESKQLGEVQVTGMAAQMIVKVDTIEYNAAAFKTAENAVVEDLLKRLPGVQISD
                     GSITVHGEKISRIKVDGKKFFDGDIEMTTRNLTADMIDKIQVVDEKSDMAKLTGFEDE
                     NTERIINLQLKPNRRNGVFGNVGAAGGADIDNGFNYDASTFLDDDFRYNANAFLNIMS
                     ANAQTAITGGANNINSSFSGRGRGFRGWGGNNGITQTQNLGLNNNTQIKEGLIIGGDA
                     SYNHSSNHSKTESSRDSWLSGDTISNNNKSVSNSFSDNTNLRFEMEWKPDSFNTLIVQ
                     PSISYSLNQTDRYQDYDYYNNGDSTSWGNSDNLSNSTNLNSRLNLIYSHRSKQKPGRS
                     ITVNLGGNYGESNSTGYNKSIKETPDSVVNIDQKSLNTSGSYGLNMRTSYVEPLWNLQ
                     NLIEASVSFNYSGRDSEKEQFDNDGLGNYPLENKDLEYSNSFKNYNFTQVAELNYRYV
                     SQVWNIMAGFRAEPAQTFSYTTYGDGTYYEIPQSVLNFSPSLSVRYVLGERRNFIRME
                     YRGRTQEPSVTQMQPVKNNSDLMNEMVGNAHLVPSYSQNLRLIMSKYNPETFASWNAT
                     LFGDITKDALVANSIYDLSGKQYNQTVNADRPPFNLNGRFMYNTPLIPNRLHFNTGTS
                     AGYTERIGYSSRKVSDLDVNNLPLGARSLTKIYRFSEDLSLTFTHDIIEIGARGSFSY
                     NNSLNNLNNTEQTTLNWSGSGNVNLHLPKSINVSTDISYSDRYGYANFDQTEILWNAT
                     IDKTIKNKLTVSL"
     gene            2855..3700
                     /gene="kduI"
                     /locus_tag="PLI37_04400"
     CDS             2855..3700
                     /gene="kduI"
                     /locus_tag="PLI37_04400"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HOQ58286.1"
                     /translation="MKTNYELRYAAHPRDVKRYDTEELRRDFLIENLFVANEVNMVYS
                     MYDRMIVGGALPKGESLKLEAIDPLKAPCFLHRRELGIYNVGLGEGIVKVGDNQFTLK
                     YKEALYLGAGDREVYFESKDAANPALFYFNSLVAHKTYPDKKITKENAIVAEMGSLES
                     SNHRNINKMIVNQVLPTCQLQMGMTELAPGSIWNTMPAHIHSRRMEAYFYFDLPEDHA
                     ICHFMGEPTETRHIWMKRNQAVLSPEWSIHSAAATNNYTFIWGMGGENLDYGDQDFFT
                     FTELR"
     gene            3719..4498
                     /locus_tag="PLI37_04405"
     CDS             3719..4498
                     /locus_tag="PLI37_04405"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HOQ58287.1"
                     /translation="MNPFSLKGKIALVTGASYGIGMSIAKAYAQAGAKIVFNDIKPEL
                     VEKGIEEYKASGFDVKGYVCDVTDEAQVNAMVAQIEKEVGVIDILVNNAGIIKRIPAV
                     DMTAAEFRQVIDVDLNGPFIVSKAVAPGMIKKKAGKIINICSMMSELGRETVAAYASA
                     KGGLKMLTRNLASEWGEFNIQVNGLGPGYIATPQTAPLRTPGHPFNEFIIAKTPAARW
                     GTPEDLQGPAVFLASSASDFVNGHILYVDGGILAYIGKQPK"
     gene            4526..5647
                     /locus_tag="PLI37_04410"
                     /pseudo
     CDS             4526..5647
                     /locus_tag="PLI37_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312824.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
     gene            5895..7766
                     /locus_tag="PLI37_04415"
     CDS             5895..7766
                     /locus_tag="PLI37_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018920374.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOQ58288.1"
                     /translation="MNNNAGFTTLDYIIFIAYAVMIVGLGLWLSRSKKGEERDSKDYF
                     LAGGKLSWWAIGASLIAANISAEHFIGMSGSGYRIGLGIAAYEWLAAITLILVAKYLL
                     PIMLDRKIFTIPQLARERYGKGVSFFFSFFWLLVYVFVNLTSVAWLGALAMNQILGIP
                     QMYGVIGLLLFSGLYSIYGGLKAVAWTDVLQVIFLVGGGLITAWFALAAVSGDANSAI
                     EGFKVIFTKIKETEGDLHFSMIIDQNLDGERFKELPGLAVIFGAMWLTNLGYWGFNQY
                     IIQKGLAAKNLKEAKKGLIFAGYLKILIPLIVVIPGITAFVMFQHQADFPGLQETLSG
                     TIGRADDAYPWLLRNFAPVGIRGLAFAALVAAVVSSLASMLNSTSTIFTMDIYKQQIN
                     KNASEKQLVLVGRLTALIALIVAVVSAKPLLGGMDQAFQYIQEFTGFIYPGVVVVFGM
                     GLLWKRATNTSALWTTIATIPVGIIMKVALPDLPFILRMGYVCMILISMAVIITFLDK
                     KNRVPADPISPEKRKGLLKAGWFFAITAGITLICAVIFGTNWFGLSLRHIGFHSILLL
                     TFLLGFLAIMMFTNANSVQKDAKSYEMNTELFKTDTGFLLGAMGIVVIIATLYAVFW"
     gene            7853..8542
                     /locus_tag="PLI37_04420"
     CDS             7853..8542
                     /locus_tag="PLI37_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOQ58289.1"
                     /translation="MDSFYKEHPRFFVSVDCLVFGFANGQLNLLVCKRKMEPAQGTWS
                     LPGGFIEQDESAEAAAERVLSNLTGLEDIYMEQLKMFSQPKRDPGERVLSVAFYALID
                     LGQEALEIKPSYQAHWFNINELPDLIFDHPEMVDLALKTLRHAVSTKPIVFNLLPEKF
                     TLPQMQTLFEAIYDQALDKRNFRKKVQSMPFLVKLNEKDKQSSRKGAFLYTFDQKEYE
                     RASQNGFCFSL"
     gene            8588..9274
                     /locus_tag="PLI37_04425"
     CDS             8588..9274
                     /locus_tag="PLI37_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOQ58290.1"
                     /translation="MELSLLKEKVFQANLDLVKHGLVIFTWGNVSAIDREKGLVVIKP
                     SGVAYEDMRVEDMVVLDLNGKKLDGKLKPSSDTATHLELYKAFPEIGGVVHTHSTFAT
                     AWSQAGMSIPNIGTTHADYFSREIPCTRDMDEEEVMGEYEKQTGLVIVEAFKDINPVQ
                     VPGVLVKNHGPFAWGKSADDAVHNAVVMEQVAKMAYIAFSINPELTMNPLLIQKHFFR
                     KHGPGAYYGQ"
     gene            9315..10817
                     /gene="araA"
                     /locus_tag="PLI37_04430"
     CDS             9315..10817
                     /gene="araA"
                     /locus_tag="PLI37_04430"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOQ58291.1"
                     /translation="MNYRDLEVWFITGAQLLYGGDAVVSVDAHSNEMVKGLNDSGRLP
                     IKVVYKGTVNSSDEISAAFRAANADTGCIGVITWMHTFSPAKMWIHGLMDFKKPLLHL
                     HTQYNEKIPWDTMDMDFMNLNQSAHGDREFGHITSRLRKPRKVVVGYWKDKATQDKIA
                     VWMRVCVGWADAQNMRIIRFGDNMNNVAVTDGDKVEAEIRLGYHVDNAPIAKLVPYVK
                     AVTEAEIDALVAEYDKLYDIAPDAAKGGKNHIHLRAAAAQEIGLRRFLDDKGAKAFTT
                     NFDELEGMDQLMGFASQRLMAEGYGFGAEGDWKTAALVRTMYVMSLGLEGGGSFLEDY
                     TLNFDGENTSVLQAHMLEISPAISKAKPKIEVHFLGIGDAGTCARLVFTAHEGEGIAC
                     TIVDMGNRFRMIVNDVKVIKPKALPKLPVARALWIPQPNFEVGAGAWILAGGTHHSSF
                     SFALTKEYMEDYAEIADIELLLIDKDTTIRQFKKDMRINEVYYMLNKALR"
     gene            10828..12504
                     /locus_tag="PLI37_04435"
     CDS             10828..12504
                     /locus_tag="PLI37_04435"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009160959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOQ58292.1"
                     /translation="MKKYVIGLDYGSDSCRALVVDAHTGEELGTSVRYYPRWAEGKYC
                     DPIINQYRQHPKDYLECLEASVKEALADSGPGVAENVIGMSFDTTGSTPVFIDKQGLP
                     LSLSPEFAENPNAMFILWKDHTAIKEAAEINELAKKWEIDYTAYEGGVYSSEWFWAKI
                     LHVLRNDKSVRKAAYSVAEHCDWLPAYICGTAQPDKMKRSRCAAGHKAMWHEKWGGLP
                     SEKFLTKLDPLLAGYRDRLYKETYTSDTCVGHLSEEWAKRLGLTTKVAVGVGAFDCHM
                     GAVGAEIAPNTWVRVIGTSTCDIVIASYKEMGDKLVKGICGQVDGSVIPGYIGLEAGQ
                     SAFGDIYAWFKRVLSWPVEEIVAKSDLLDAETKQKLVEEALDKIIPVLTEQAAEVDPN
                     QSTVFATDWMNGRRTPDASQLVMGTISGLTLGSTAPMIFRALVEASAFGSKAIADRFD
                     QEGVKIDTIIGIGGISLKSPLVMQIMADVLNQPIKVARCEQTCAFGAAMFAAVVAGVY
                     DKVEDAQKSMGQGFTEEYHPNPEMARFYEAKYREYRQLGELTEKAFYSTI"
     gene            12520..>13910
                     /locus_tag="PLI37_04440"
     CDS             12520..>13910
                     /locus_tag="PLI37_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOQ58293.1"
                     /translation="MNSTALMNKAADNIRILAASMVEKAKSGHPGGAMGGADFINVLY
                     SEYLIYDPNNPAWEGRDRFFLDPGHMSPMLYSVLAMSGKFSMDELAQFRQWGSPTPGH
                     PEVDVQRGIENTSGPLGQGHAYAVGAAIAAKFLQSKFGEQMNQTIYTFISDGGVQEEI
                     SQGAGRLAGHLGLDNLVMFYDSNDIQLSTEVSAVTSEDTAAKYKAWGWKVLEINGNDV
                     NDIRKALDEAKKTSGKPTLIIGKTIMGKGCLTEEGKSFENQCSTHGMPLSQAGASYDK
                     TIENLGGNPADPFRIFPEVETLYANRRKELEAIMKDKKAKKAAWAAQQPALAKKMQDW
                     FNKVVPAVNWEAIEQKEGIATRAASATVLGALATQVENMVVASADLSNSDKTDGFLKK
                     TKAMTKDDFSGAFLQAGVAELTMACVCIGMGLHGGVIPACGTFFVFSDYMKPAVRMAA
                     LMEVPVKFIWTHDA"
BASE COUNT         3710 a         3067 c         3311 g         3822 t
ORIGIN      
        1 agcgacacgg ttaatttgtt tttgatggtt ttgtctatgg ttgcattcca caaaatttcg
       61 gtctggtcga aattggcgta accgtaacga tcgctgtaac tgatatcggt cgaaacattg
      121 atgcttttgg gtaaatgcaa attcacatta ccgctgcccg accaattaag agtggtttgt
      181 tcggtattat tcaagttatt cagggaattg ttataactaa aagaacccct ggcgcctatc
      241 tctataatat cgtgggtaaa agtcaaagac aaatcttcgc tgaatctgta aatcttggta
      301 aggctgcggg ctccaagcgg cagattatta acatccagat cgctcacctt tctgcttgaa
      361 taaccgattc tttccgtgta accggccgat gtgccggtgt taaaatgcag ccggttaggt
      421 atcaggggag tattatacat aaatcttccg ttcaggttga aagggggacg gtcggcatta
      481 acagtctggt tgtattgttt tccgctgaga tcgtaaatac tgttggctac caaagcatcc
      541 ttggtgatat cgccaaataa agtggcattc cacgaagcga aagtttcggg attgtatttg
      601 gacataatca acctcaggtt ttgactatac gaggggacta aatgggcgtt acccaccatt
      661 tcgttcatca ggtccgagtt gtttttgacc ggttgcatct gggtcaccga aggttcctgg
      721 gtgcggccac gatattccat acgtataaaa tttctgcgtt ctcccagtac atagcgtaca
      781 ctcaaagatg gagaaaaatt caacacactt tgcggaattt cgtaataggt gccgtctccg
      841 taagtggtat aactaaaagt ttgggcgggt tctgctctga aaccggccat aatattccat
      901 acctggctga cgtaacggta attcaattcg gctacttggg taaagttgta atttttgaaa
      961 gagttgctgt attccaggtc cttgttttcg agaggatagt ttccaagtcc atcgttatcg
     1021 aattgttctt tttcggaatc ccggccggaa taattgaacg aaacggaggc ttcgataaga
     1081 ttctgcaaat tccacagagg ttctacataa gacgttcgca tgttcaggcc gtaagaaccg
     1141 gaggtgttga ggctcttttg gtcgatatta acaacagaat cgggtgtttc tttgatcgat
     1201 ttgttgtaac cggtactgtt ggactctccg tagtttccac ccaaatttac ggtaatggag
     1261 cgaccgggtt tttgctttga tctatgacta taaatcaaat ttaaccggga attcaggttc
     1321 gttgaattgc tcaaattatc cgaattaccc caactggtgc tgtcgccgtt gttgtaataa
     1381 tcgtagtcct gatatctgtc tgtttgattc agactgtagg atatggatgg ttgtacgatg
     1441 agggtattga atgagtcggg tttccattcc atctcgaacc ttagattggt attgtcgctg
     1501 aatgagttgg aaacactctt attgttgttg ctgatggtat ctccgctcag ccaactgtct
     1561 ctggaacttt ctgtttttga atgattgctg gaatggttgt aggaagcgtc tcctccaatg
     1621 atcaggcctt ctttgatctg tgtgttgttg ttcagaccaa ggttttgggt ttgggtaata
     1681 ccgttgttgc cgccccagcc gcggaatcca cggcctctgc cgctgaaact gctgttgatg
     1741 ttgttggctc cgccggtaat ggccgtctgt gcattggccg acattatatt aaggaaagca
     1801 ttggcgttgt aacgaaaatc gtcgtcaagg aaggtagagg catcgtaatt gaatccgttg
     1861 tcgatgtcgg ctcctccggc agcgcctacg ttgccgaaaa caccgttacg tctgttgggt
     1921 ttcaattgca aattaataat gcgttcggtg ttttcatcct caaacccggt caatttggcc
     1981 atatccgatt tttcgtccac tacctgaatt ttatcgatca tatcggcagt caggtttctg
     2041 gtagtcatct cgatatcgcc gtcaaaaaat ttctttccgt ctactttgat ccgcgatatt
     2101 ttttcaccgt gaacagtgat gcttccatcg gagatctgca ctccgggcaa acgtttgagt
     2161 agatcttcga ctaccgcgtt ttcggccgtt ttaaaagctg ccgcattata ttcaatggtg
     2221 tccactttga ctatcatttg ggctgccatg ccggtcacct gcacttcgcc caattgtttg
     2281 gattcttctt ccatgaatat gtttggtagt gtgatggacc tatctttgat ctctatgtct
     2341 ttgtaaacgg ttttataacc cagaaaacgg cattccaata tgtattttcc ggcgctgatt
     2401 tttgttaaag taaaacgtcc tgcctgatcg ctcaaagtac ccgcaacaag actggaatct
     2461 cccggggtca gcaagcggac agagcccatt tctatagccc cgtcggtagc tttgtccaac
     2521 atttttgatt gtatactgta ttctgctgca aacgcaacga gcgggctaag taaaagcagg
     2581 agcagagcaa acatttttat tctcataaaa accgtaatgt gaaaaacaga tggattttca
     2641 ataaaacaac ggctctttga tgcctggttt agaaaatagt ttaatcccaa gagaaaattt
     2701 actttacaat taatgttaat acaacccgat tagttgaagt cgatttagtt aaaaatgaaa
     2761 aataatggga agcttttcca tgtataaaca gatttatgtt acttttgcaa cttatgaata
     2821 aaaggtcttt ttacacttaa aacgatattg tattatgaaa accaactacg aactccgcta
     2881 tgcagcgcat ccccgggatg taaaacgtta tgacactgaa gaacttagaa gggatttcct
     2941 gatcgaaaat ctttttgttg ccaacgaagt aaacatggtt tattccatgt acgacagaat
     3001 gatcgtaggt ggcgccctgc ccaaaggaga atctctgaag ctcgaggcaa tagatcccct
     3061 gaaagctccc tgttttttgc accggcgcga attaggcatc tataatgtgg gcctgggcga
     3121 aggcattgta aaggtggggg acaatcaatt tactttaaaa tataaggagg ctctttatct
     3181 gggtgccggc gacagagaag tatacttcga aagcaaagac gccgctaatc ctgctttgtt
     3241 ttacttcaac tctctggtgg ctcacaagac atatccggat aaaaaaatca ccaaagaaaa
     3301 cgctattgtc gcagaaatgg gcagcttgga aagttccaac cacagaaata tcaacaagat
     3361 gatcgtgaat caggttttac ctacctgcca actgcagatg ggaatgaccg agcttgctcc
     3421 cggaagcatt tggaacacca tgcctgctca cattcacagc cgccgtatgg aagcgtattt
     3481 ttattttgat ctgcccgaag atcacgctat ttgtcatttc atgggagaac ccaccgaaac
     3541 ccgccacatt tggatgaaac gcaaccaggc tgtactttcg ccggaatggt ctattcattc
     3601 agctgctgcc actaataatt ataccttcat ttgggggatg ggcggcgaaa atctcgatta
     3661 cggagatcag gatttcttca cttttaccga attacgttaa ttataaaaca gcatatatat
     3721 gaatccattt tcattaaaag gaaaaattgc actggtaacc ggtgcatcct atggtattgg
     3781 aatgtctatt gccaaggctt acgcacaagc cggtgccaaa attgtcttca acgatattaa
     3841 acccgaattg gtcgaaaaag gcatagaaga gtacaaagct tcaggttttg atgtcaaggg
     3901 ttatgtttgt gatgttaccg atgaggctca ggtaaatgcc atggtggccc aaatcgaaaa
     3961 agaagtgggc gttatcgata tactggtaaa caacgccggt atcattaagc gtattccggc
     4021 cgtcgatatg accgctgctg aatttcgtca ggtgatcgac gtcgacctga acggcccttt
     4081 tatcgtatcc aaagccgttg ctcccggtat gataaagaaa aaagccggta aaatcatcaa
     4141 tatctgttcc atgatgagtg aattgggacg cgaaaccgta gcagcttacg cttcggccaa
     4201 aggcggtctt aaaatgctga cccgcaatct ggccagcgaa tggggagaat tcaacattca
     4261 ggtgaacgga ctgggaccgg gctatattgc tactccgcaa acagctcctc tacggactcc
     4321 cggacatccc tttaacgaat ttattattgc caaaactccc gcggcccgct ggggtacacc
     4381 cgaagatttg cagggaccgg ccgttttctt ggcttcttct gcttcggatt ttgtgaatgg
     4441 tcatatcctt tacgtggacg gcggcattct ggcttacatc ggtaaacaac ccaaataacg
     4501 ttcgataaat cttaattatt aagccatgaa aaagcttgta tttttactgt cgtcagcggt
     4561 gttattgctg aatgcctgca ccagttacga cgcccccaag gcgaaactca atcgtgttga
     4621 cttccagacc gagatcgatg gaaaacaaaa cgatttgtac atcatgaaaa atgctgcggg
     4681 tatggaagtt tgcatgatga attacggagc ccgcattgtt tctgtgatgg tgcccgatca
     4741 aaagggtaaa ttcggcgacg tagtcctggg ttttgacaat attcaggatt acctggacaa
     4801 ttctaccgat tttggcgctt tcatcggccg ttacggtaat cgcatcaatc agggtaaatt
     4861 ctgcctggac ggcatagaat accaattatc cgtaaacaat ttcggacata gtctccatgg
     4921 cggagttaaa ggtttccaat actgtatgtt cgacattaaa caattgagcg agcagagcct
     4981 ggaggtttct tatgtatccg aagacggaga aatgggtttc cccggtcaac tcacagtaaa
     5041 ggccgtttat actcttaccg acgacaatgg cattgatatt cgctacgaag cggaaaccga
     5101 caaacctacc atagtcaatc tgaccaacca ctcttatttc aatctgagtg ccgatccttc
     5161 gcaaagcata acggatcata ttctgatgat cgatgccgat gcctttactc cggttgattc
     5221 gacctttatg accaccggtg aaatcgctcc cgtgtgcaat actcctatgg attttcgtaa
     5281 gcccgaagtt attggaaaac gcattgatga ctttagtttt gagcaactta aaaacggtaa
     5341 cggctacgac cataattggg tgttaaatac cgaaggagat gctaccaaat tagcctgcct
     5401 gcttcttgat cccaaaagtg gacgtatact taaagtgttc acctcggaac ccggagtaca
     5461 agtatacacc ggtaattttc tggatggcag cctgaggggc aaaaatggtg ttgtctataa
     5521 tcaacgtgct gctatctgtc ttgaaaccca acattatccc gacagtccca acaaggccga
     5581 ctggccatcg gtagtgttgc gtcccggtga aaaatacacc agccattgca tctatcaatt
     5641 tgcggtaaaa tgcagcaacg agggtaaaga gtgttgcggc aaaaacaaga aatgctgcaa
     5701 agagggggcc gattgtaagg aagggggagc ctgctccgat tcttgcagcc aaaaaccctg
     5761 cggaaaagct gaaggaaaac cctgtgccgg ctctgaagca aagcctggcc ggaacagaaa
     5821 caaagctcta caatgaggaa taaataagat atcacttttt aaaaacctta atattaacca
     5881 attaattttt tgttatgaat aataacgctg gttttacaac cctcgactac atcatcttta
     5941 tagcgtacgc tgttatgatt gtagggctcg gtctttggct ttcacgctcc aaaaaaggag
     6001 aggagagaga ctccaaggat tattttcttg ccggtggcaa gctttcctgg tgggcaatag
     6061 gtgcctctct gattgcggcc aacatttcgg ctgaacattt tatcggaatg tcgggttcag
     6121 gttatcgtat tggtttaggt attgccgctt atgagtggct ggcagccatc accttgattt
     6181 tggtggcaaa atacctgctg cccattatgt tggacaggaa aattttcacc atacctcaac
     6241 tggcccgtga acgttacgga aaaggagttt ccttcttttt ctccttcttt tggctcttgg
     6301 tttacgtttt tgtcaaccta acctctgtag cttggttagg agcactggcg atgaatcaga
     6361 ttttgggcat accccagatg tatggagtaa tcggcctgtt gttgttttcc ggtctttatt
     6421 ccatttacgg cggtctgaaa gcggtagcct ggaccgatgt gttgcaagtg atcttcctgg
     6481 ttggtggtgg tcttattact gcttggtttg ctctggctgc cgtcagcggc gatgccaaca
     6541 gcgccatcga aggtttcaaa gtaatattca ctaagattaa agaaaccgaa ggcgacttgc
     6601 atttcagcat gatcatcgat cagaacctcg atggagaacg cttcaaagaa ttgcccggcc
     6661 tggctgttat tttcggcgcc atgtggctca ccaatttggg ctattgggga ttcaaccagt
     6721 acattattca aaaaggcttg gctgctaaaa atttgaagga agctaaaaaa ggattgattt
     6781 ttgccggata cctcaaaata ttgatccctc ttatcgtggt aatccccggc atcactgcct
     6841 ttgtcatgtt tcagcatcag gcagactttc ccggtttaca ggaaacactc tcaggcacca
     6901 ttggaagggc ggacgatgcc tatccctggt tgttgcgtaa ttttgcccct gtgggtattc
     6961 gtggtctggc atttgccgcc ctggttgcag ccgtggtctc ttcactggct tcgatgctga
     7021 attccacttc gaccattttc accatggaca tctacaagca acagatcaac aaaaacgcca
     7081 gcgaaaaaca attggttctc gtaggtcgtt taaccgcgtt gattgctttg attgttgccg
     7141 ttgtttcggc taaaccctta ttgggaggaa tggatcaggc tttccagtat atccaggagt
     7201 ttacgggctt tatttatccg ggcgtggtgg tagtatttgg tatgggtctt ctttggaaaa
     7261 gagcaaccaa cacttctgcc ttatggacca ccattgcgac tataccggtt ggcattatta
     7321 tgaaagtggc cctgcctgac ttgcccttta tccttcgtat gggttatgtc tgtatgattt
     7381 tgatttcaat ggccgtaatc atcactttcc tcgataaaaa gaacagggtt cctgccgatc
     7441 ccatatcgcc cgaaaaacgc aagggtctgc taaaagccgg ttggttcttt gccatcactg
     7501 ccggtattac tcttatctgc gcagttattt ttggaacaaa ttggtttggt ctcagcctca
     7561 gacatatcgg tttccactct atcttactgt tgaccttcct tctgggcttc cttgctatta
     7621 tgatgtttac caatgccaat tccgtccaaa aggacgcgaa gtcttacgaa atgaataccg
     7681 aactattcaa gacagacact ggcttcctgt tgggagctat gggaatagtc gtcatcattg
     7741 ccactctata cgccgtattc tggtaaatct ttttcaagtg aacccggttt tttcccgggt
     7801 tcacttgatt atatagagct tatccggttt tatacctagg catttgaaag gtatggattc
     7861 attttacaaa gagcatcccc gtttttttgt atccgtcgat tgtctggttt ttggttttgc
     7921 aaacgggcaa ttgaatctat tggtgtgcaa acgcaaaatg gaacctgctc agggtacctg
     7981 gtctctgccg ggaggattta tcgaacaaga cgagagcgcc gaagctgccg ctgaacgagt
     8041 cttatccaat ctgaccggac tcgaagacat ttatatggag caattgaaaa tgtttagtca
     8101 gcccaaacga gatccgggag agagggtgct ttccgttgct ttttatgcct tgatcgacct
     8161 ggggcaggag gctttggaaa taaaaccatc ttatcaggca cattggttca atatcaacga
     8221 actgcccgac ttgatttttg atcatcccga aatggttgat cttgctctga agacattgag
     8281 acacgctgta tcaaccaagc cgatagtttt caatctcttg ccggaaaaat tcactctccc
     8341 tcaaatgcaa actttgtttg aagcaatcta tgaccaagct ttggataaac gcaattttcg
     8401 caagaaagtt cagtccatgc cctttttagt taaattgaat gaaaaggaca aacagagttc
     8461 aagaaaggga gcctttttat atacctttga tcaaaaagag tacgaaagag caagtcaaaa
     8521 cggcttttgc ttcagcctgt aagtgattta acttataaaa attataagaa catcgaattt
     8581 taactctatg gaattgtcgt tattaaaaga gaaagtattt caggccaacc ttgatttggt
     8641 taagcacggc ctggttattt ttacctgggg caacgtgagt gccattgaca gggaaaaagg
     8701 actggtggtg ataaaacctt cgggagtagc ttacgaggat atgagggtcg aagacatggt
     8761 agtgctcgat ctgaatggga aaaagctaga tggtaagctc aaaccctcgt ccgatacggc
     8821 cacccacctg gaattgtaca aagcttttcc cgaaataggc ggtgtggtgc atacccattc
     8881 tacttttgca actgcctggt ctcaggccgg tatgtccatt ccgaatatag gcactaccca
     8941 tgccgattat ttcagcagag aaataccctg tacccgcgac atggatgagg aagaagtgat
     9001 gggggaatac gaaaaacaaa ccgggttggt tattgtagaa gccttcaaag acatcaatcc
     9061 ggttcaggtg ccgggggttt tggttaaaaa ccatggtcct ttcgcctggg gcaagtcggc
     9121 tgacgatgcc gtacacaacg ccgttgttat ggaacaggta gccaaaatgg cttatatagc
     9181 cttttcaatc aatcctgaat tgaccatgaa ccctttgctc attcaaaaac atttctttcg
     9241 caaacatggc cccggtgcct attatggtca atgaacaata gaaaacagta ttaataaata
     9301 aaaatttcac tactatgaat tatcgcgatc ttgaagtatg gttcattacc ggtgcacaat
     9361 tgttgtacgg tggtgatgca gttgtgtctg ttgatgcaca ttccaatgaa atggtaaaag
     9421 gtttgaacga ctccggcaga ctgcctatta aagtggttta taaaggcaca gtcaattcgt
     9481 ccgatgaaat atctgctgcg ttcagagctg ctaatgccga caccggttgc ataggtgtta
     9541 ttacatggat gcacactttt tctcctgcca aaatgtggat acacggcctg atggatttta
     9601 agaagccctt gctgcatctg catacccaat acaacgaaaa gataccttgg gataccatgg
     9661 acatggattt catgaatctg aatcaaagtg cacacggaga ccgtgaattt gggcatatca
     9721 ccagtcgtct tcgcaaaccc cgtaaagtgg tggtcggcta ttggaaagac aaagccactc
     9781 aggacaaaat tgccgtctgg atgcgtgttt gtgtaggttg ggccgatgct cagaatatgc
     9841 gtatcattcg ttttggcgac aatatgaaca acgtggccgt gaccgacggc gacaaagtgg
     9901 aagccgaaat acgcttgggt taccatgtag acaatgctcc cattgctaaa ctagtacctt
     9961 atgtcaaagc cgtcactgag gccgaaatcg atgctttagt ggcagaatac gataaattat
    10021 acgacattgc tcccgatgca gccaagggcg gtaaaaacca catccatttg cgtgccgctg
    10081 ccgcacagga aattggcctg cgccgtttct tggacgataa aggtgccaaa gcttttacta
    10141 ccaattttga tgaactggaa ggcatggacc aactcatggg ttttgcttct caacgcctaa
    10201 tggccgaagg ttacggtttc ggtgccgaag gtgactggaa aacggctgcc ttggttagaa
    10261 ccatgtacgt tatgagcctg ggcctggaag gcggcggttc cttcctggaa gactacacgt
    10321 tgaattttga cggcgaaaat acctctgtgc ttcaggccca tatgttggaa atcagtcccg
    10381 ctatttcgaa agcaaaaccc aaaatagagg tgcatttcct gggtatcggc gatgcaggta
    10441 cctgtgcacg tctggtcttt actgctcacg aaggcgaagg aatagcctgt accatagtag
    10501 atatgggcaa tcgtttccgt atgatcgtaa acgacgtgaa agtgatcaaa cctaaggctt
    10561 tgcctaaact gcccgtggca agagcgctct ggattcctca gcccaacttc gaagtgggag
    10621 ccggagcctg gatattggcc ggtggtactc accattccag tttttctttc gcgctcacca
    10681 aagaatatat ggaagattac gccgaaattg ccgatatcga attgctcttg attgacaaag
    10741 acacaaccat tcgtcagttc aagaaggata tgcgtatcaa cgaagtctat tatatgttga
    10801 acaaagcttt acgctaaatt attgattatg aaaaaatacg taataggact ggattacgga
    10861 agcgactcat gtcgtgctct ggtggtcgat gcccacacgg gtgaagaatt gggtacctct
    10921 gtacgctatt accccaggtg ggccgaaggg aagtattgtg atccgataat caaccagtac
    10981 cgtcaacatc ccaaggacta cttggagtgc ctggaggctt ctgtcaaaga ggctttggcc
    11041 gatagtgggc ccggcgtggc cgaaaatgtg atcggaatgt cttttgatac aacaggcagt
    11101 actccggttt ttatcgacaa acaaggactg cctttgtctt tgagccctga gtttgccgag
    11161 aatcccaatg ctatgtttat tctgtggaaa gatcatacag ccataaaaga agccgcagaa
    11221 atcaacgaac tggccaagaa atgggaaata gattacacgg catacgaagg cggtgtatat
    11281 tcttctgaat ggttctgggc aaaaatactg catgtactgc gcaacgacaa aagtgtacgc
    11341 aaagcagcgt attcggttgc agaacattgc gattggctgc ctgcctacat atgcggtacg
    11401 gcgcaaccgg ataaaatgaa acgcagccgt tgtgcagccg gtcacaaagc catgtggcac
    11461 gaaaaatggg gcggtttgcc ttcggaaaaa tttctgacca aactcgatcc acttttagcc
    11521 ggttaccgag accgcctcta caaggagact tacaccagcg atacttgcgt tggccacctc
    11581 agcgaagaat gggctaaacg cctgggcttg accactaaag tggctgtcgg tgtcggcgct
    11641 ttcgattgcc atatgggtgc cgtgggagca gaaatagcac ccaacacctg ggtaagagta
    11701 atcggcacat ccacctgcga tatagtgatc gcttcttaca aggaaatggg cgacaagctg
    11761 gttaaaggta tttgcggaca ggtagacggt tcggttattc ccggatatat tggtctggaa
    11821 gccggacagt cggcttttgg cgacatttat gcctggttca aacgtgtcct gtcctggccg
    11881 gtagaagaaa tcgttgccaa gtcggatttg ctggacgctg agaccaagca aaagctggtg
    11941 gaagaagcct tagacaaaat aatccccgtg cttacggagc aagctgccga agtcgatccg
    12001 aatcaaagca ccgtgtttgc caccgactgg atgaacggac gacgtactcc cgatgccagc
    12061 caactggtta tgggcactat ctccggactg actttgggtt ctacggcccc tatgattttc
    12121 agggccctgg tcgaagcctc tgcttttgga tccaaagcca tcgccgaccg cttcgatcag
    12181 gagggggtta agatcgacac catcatcggt attggcggaa tttcattaaa atcgcctttg
    12241 gttatgcaaa ttatggccga cgtgctcaat cagccgatta aagtagcgcg ttgcgaacaa
    12301 acctgtgctt ttggtgccgc tatgtttgct gctgttgtgg ccggtgtata tgacaaggtg
    12361 gaggatgcac aaaaatctat gggtcaaggc tttaccgagg aatatcaccc caatcccgaa
    12421 atggctcgct tctacgaagc taaataccgg gaatatcgtc aattgggaga attgaccgaa
    12481 aaagcatttt attcaactat ttgattttaa ttttactata tgaattctac tgcattaatg
    12541 aacaaagcag ccgacaatat tcgcattttg gctgcatcta tggttgaaaa ggcgaaatcc
    12601 ggccatcccg gaggggcaat gggcggagct gatttcatca acgtacttta ttccgaatac
    12661 ctcatttacg atcctaataa tcccgcttgg gaaggacgcg accgcttctt tctggatccc
    12721 ggacacatgt ctcccatgtt gtactcggtg ctggccatga gcggaaagtt cagcatggat
    12781 gaactggctc agttccgcca atggggcagc cctacaccgg gccatcccga agtggatgtg
    12841 caaagaggca tagaaaatac ctcaggcccc ctgggtcagg ggcatgccta tgccgtaggt
    12901 gccgccattg ccgcaaaatt tctgcagagc aaatttgggg aacagatgaa tcaaaccatc
    12961 tacaccttta tttccgacgg aggtgtacag gaagagattt ctcagggagc cggacgtttg
    13021 gcgggtcatt tgggtctgga taatctggtg atgttttacg actccaacga tattcaactt
    13081 tcgacggaag tatcggcagt taccagtgaa gatacggctg ccaaatataa agcctggggt
    13141 tggaaagtgc tggaaataaa cggaaacgat gtcaacgata ttcgtaaagc actggatgaa
    13201 gccaaaaaaa ccagcggaaa acccactctg attattggta agaccatcat gggtaagggc
    13261 tgtcttacag aagaaggcaa atccttcgaa aatcaatgtt ccacccatgg tatgcctctg
    13321 agtcaagccg gagccagtta cgataagacc atcgaaaatt tgggaggtaa tcccgccgat
    13381 cctttccgta ttttccccga agtagaaacg ctctacgcca acagacgcaa agagttggaa
    13441 gcaataatga aggacaaaaa agccaaaaaa gcagcttggg ctgcccagca acccgcactg
    13501 gctaagaaaa tgcaggattg gttcaataag gtcgttcccg ccgttaactg ggaagccatc
    13561 gagcaaaaag aaggtatcgc tacccgcgct gcctccgcta ccgtgcttgg agcactggct
    13621 actcaggtgg aaaacatggt ggtggcttcg gcagatttgt cgaattcaga caagaccgat
    13681 ggtttcctca aaaaaaccaa ggccatgacc aaagacgatt tcagcggagc ctttctgcaa
    13741 gccggtgttg ccgagcttac catggcttgc gtctgtatcg gtatggggct gcacggaggt
    13801 gtgattcccg cctgcggaac gttctttgtt ttttccgact acatgaaacc ggctgttcgt
    13861 atggcggctt tgatggaagt tcccgttaaa ttcatctgga ctcacgatgc
//
LOCUS       DAOSTD010000045        13812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_4521_length_13812_cov_14.012575, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000045 DAOSTD010000000
VERSION     DAOSTD010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13812
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4521_length_13812_cov_14.012575"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..332
                     /gene="rpsO"
                     /locus_tag="PLI37_04445"
     CDS             63..332
                     /gene="rpsO"
                     /locus_tag="PLI37_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOQ58294.1"
                     /translation="MYLTKEKKQEIFEKYGKSNTDTGSAESQIALFSYRISHLTEHLK
                     SNRKDYSTERSLKMLVGKRRRLLDYLKEKDIEKYRALIKDLGIRR"
     gene            464..2791
                     /locus_tag="PLI37_04450"
     CDS             464..2791
                     /locus_tag="PLI37_04450"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOQ58295.1"
                     /translation="MTIRFTIHYHTYPGQELLFCGSQETTAWLRDEQAKPMTYRSGGY
                     WSLDLPVRQKGVFVYRYLVRENGRITRREWGRHQLTLTSLTEQFAEVLDTWQTEPAKS
                     AGTAIPVFSLRSDKDWGIGDFGDLFPMIDWLEQSGQKILQLLPVQDTTNSFSWYDSYP
                     YSIISVFALHPIYAAPSLLPALEDRDKAASFEERAKQLNLSESVKYEEVLSLKWEYFR
                     LLFDQEQGKIIQSVDYKRFLKENSHWLRPYAAYCYLLRKLNNPDPKAWGEYSNYDSES
                     ISRLCDPRQSWHKDIAIHYYIQFQLHCQLEQVHDYAAQKGILLKGDIPIGVNRFSVEA
                     WQHKNLFRLDLQTGAPPDEFSDQGQNWGFPVYDWNEMHRWQYKWWRKRLQHMAKYFDA
                     YRIDHILGFFRIWSIPTDSTSGLLGYFKPALPYTPKEIRAFGLDFDRQTMCKPLVDEV
                     LLGEYPGLEIFSYLRSPGNQKRHSSKKESPAPGSKPDQTGNHQRYLLKEELDTQSKVR
                     NYFGDSTQKEISRLREALCRMCTEVLFIEDKDKAGRYHPRILGNQTAAYRCLSEKQQK
                     AFDRLYEHFYFERHNEFWKEQAMRSLPELLSSSSMIACGEDLGMIPASVPEVMEQLCM
                     LSLELERMPKTFGVEFEALDRIPYLSVCTTSTHDMSPLRTWWEENPLQAGRYWQSVLH
                     EQGDAPLECSADLAYRILQHHLSSNAMWVILPWQDWLALHNDLRKTRAETERINVPEN
                     SKHRWDYRIHINIEDFLNSTTLNTTIRRMIADSGR"
     gene            complement(2864..3316)
                     /locus_tag="PLI37_04455"
     CDS             complement(2864..3316)
                     /locus_tag="PLI37_04455"
                     /inference="COORDINATES: protein motif:HMM:NF025425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HOQ58296.1"
                     /translation="MNQAYAFDGSESTKTSKEAETFKTAIENRQFVFQAQTAHPMAWR
                     SVNLSYGFHLKVSPDNIEAYLPYYGRAYKAPLSYEESGVKFNSHDFDYQVKEGKDGRL
                     DITIKPKDVTGKNYILYLSAFPEGSCSLSVQDPDRQHIHFNGILELPE"
     gene            3732..7070
                     /locus_tag="PLI37_04460"
     CDS             3732..7070
                     /locus_tag="PLI37_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ58297.1"
                     /translation="MNLVSFFRRVFVTSFLAATCLFAFAQEQMVSGVVKDAAGEPLIG
                     VNVATGDRGTVTDLNGAYSLSVAADGQLVFSYIGYLSQTIAINGRSVINVTMEEDVRE
                     LEAVVKIGYGTVRKRDLTGAVASIPGKEIATIPVANAAQALQGKLPGVNVVSQDGRPD
                     ASISIRVRGGGSISQSNEPLYVVDGFPVSSISDIPAEQIESIDVLKDASSTAIYGARG
                     ANGVIIVTTKSAKEGKTSITYSGYAQSKVPTKYFETLGAYDYVAYNWAYADAIGAAYR
                     DAWEMLWAIGPYAATYGNTPGIDYYKNVVAQNYQRQVYGESFSHSHNLNITGGNDKTN
                     FLVAFNHIDDDGLKVNSWYKRTNASIKLDHKLYKKLTLSLDTRFTHTNSVGNESTTNT
                     RGSVLSSSYFFRPIVTQHVMGELDPMVNSSLGMYDELLQDEFNPVARIKDYTPERNNR
                     SLRANTALNWEIVKGLVARTELGLNTYWNKNHTWTGAIFNDYYDANGNKTYSGNASIS
                     RSEGWNMRWANVLSYEIRDLGEAHHVGVTLGQEIMDSNSESMSISGSRYPASYTPERA
                     FAMMDQYDPGNLAYHSLSGSIGTPSRLMSYFARANYGLLDRYLLTVTFRADGSSRFAP
                     TNRWGYFPAGAIAWRASEENFIKEQDWVDNLKLRFSLGSVGNDGISADLWKMNWTTGG
                     LANYSINEERQVIYQPGSDVMANPNLKWETTITRDLGLDFGFLDNRVFGSLDLYYNTT
                     KDLLMLTPISDITGFSFTYDNVGSTSNRGIEVLLGGDIIKTNDFNLTASLNVNINRGR
                     VEELAEGIDGLYKTEWGSTMTRPNTGDYILEEGQPVGMVRGYTYEGWYTTSDFDYDPV
                     TGIYTLKDGVPDIAAGVIGTVYGTENNKPGSQTAYPGVIKYADISGPDGVPDGVVDES
                     DVVIIGNMNPKHTGGLNLNASYKSFDLGLFFNWSYGNQIYNANYLATIYGSKNDGLYR
                     NRMDYLAGAYKIYDIKNGNLVKVTDPAALDALNTDASLFLPYHENAVVSTLGIEDGSF
                     LRLNTATLGYTMPKNMIRKIGLVNARLYASVYNLFTLTKYSGLDPEVNTNTSQGGARY
                     PTTGLDWGAYPRARSFTFGVNLEF"
     gene            7090..9198
                     /locus_tag="PLI37_04465"
     CDS             7090..9198
                     /locus_tag="PLI37_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ58298.1"
                     /translation="MKKYIYILLGVFLLTIGTTSCGEDFLEVSSPSQVDEDFVFSTPD
                     EAYKVLMGCYEIFRGRSYVHSNGLFYDLIIGGSDSECHPESYSAQQRHVPEGLYAVED
                     FSIDFSNFQRAWRYCYQMANRLAIVIAQIETKPAFQAAWDAQEVSEWTQLYGEAVALR
                     AIAYHELIRFFGDIPYFDIPIYTQDQSDVGCTSRFVIYDDLLDDLKRVEPFMYRLGEA
                     GVNAERITGTFVQGLIGRIALYAGGYQLYRTDFDYGITLERHPNINGEKWNAIYARHP
                     QYLDYYRIAEQYLGECVSEGKATLITTDERGYGNPFQRNFQYMMDLQVSPESLFEIAE
                     TQGFQTERPYAFGRPSKGGGSNNFPCKAYGQSRIYASFYYGDFDPLDLRRDASVTVTC
                     NQHPCTEVLLLFDPGSQNKGGLANNKWDESRMSNPYTTRQRQSGINTCYMRMADVILM
                     LAEVYAELGKEGPAKTELSKVRARAFPATAKATKVDAYIAALSGDALKEAIAQERKLE
                     FAGEGLRRYDLIRTGKLPEKIVDLRKAQGAMINGLKTNGYYTFANGNTISAYIYTKPV
                     NTADLGMTYMLTKQCDVLPGDPAYPVKFPSWRGQHDGWEGFDNTSGTRNLAIQGLFEF
                     IDSASVQGKALIAAGYEVVPWGSTIVANEDQYLHDIFKGYTDADFTSKTPPRYLCPMS
                     STTISKSNGKITNGYGFSQE"
     gene            9600..12833
                     /locus_tag="PLI37_04470"
     CDS             9600..12833
                     /locus_tag="PLI37_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ58299.1"
                     /translation="MVSGVVKDAAGEPLIGVNVATGDRGTVTDLNGAYSLSVAADGQL
                     VFSYIGYLSQTIAINGRSVINVTMEEDVRELEAVVKIGYGTVRKRDLTGAVASISAKQ
                     IATIPVPNVAQALQGKMAGVNVITQDGRPDASISIRVRGGGSISQSNEPLVLIDGVPG
                     TLSDVPSDQVESVDVLKDAASTAIYGARGANGVVLVTTKGAKSGKTVVSYNGYAKFNQ
                     PTKYLETLSPYDYVSYVWANADANGINYVDSFTKLFGIGAYGDIERYRNVEAYDVQKK
                     VYNSSFSHNHDLTVSGGTDLTKIILGVNYNDEQGMKINSFHKRASVSLKVDQKINEKM
                     DLGLDTRYVDITSMSDEGTTSGSGSILSYAFRFRPIATGDILGDLNALNEGAVEQYGK
                     FCLWDRYDPYNRIRDYEPLRKRQTLRTIGSFNWELFKGLDYHTDLTLQRSWNQNKFWG
                     GPVHNSYLDDATGEVLYAGAATLYKADSWSYRWSNTLNYEIEINKANQLSVLLGQEVS
                     NSGGNGMTITADFFPANYTKENAFAMINQYDKAASTNQSPFSSSFSTPSRLLSYFTRM
                     NYSLLDRYLFAFTFRADGSSKFSPNHRWGYFPAGALGWRLSEESFLKDEDWLDNLKLR
                     ASYGSVGNDGISADLWSQNWTAETDDRWYYSIDGVAQPSYDLASSQMANPDLKWETTI
                     TRNAGIDFGVFGNRLTGTLDFYWNTTKDLLMNTVLPGITSFSSTFANIGQTSNKGVEF
                     SLNGVLFENNDWNVEAGFNINFNRNKVDELANAQELIDGKGLYYGTGWFSSGYPGNDF
                     GLYEGKPVGLVRGLVYEGMYSLDDFDYNTAEGLYYLKDGVPDISPNITGVMHGIATPT
                     GQNAYPGMAKYSDTDGSGMVDGEDFVIIGDMNAKHTGGFHITAQYKQFDLGLYFNWSY
                     GNQVYNVNRMASLMGYKETGVYQNKLAVLASSYKIYDIVNGQLVRYNTPEELAALNKN
                     AEYPLCYNESGVVSSLGIEDGSYLRLNTLNFGYTLPKSVSTKLKINNMRLFFTAYNLL
                     TLTKYQGLDPEVNANERVNHAVYPTPGLDWGTYPRPRSLVVGANISF"
     gene            12853..>13812
                     /locus_tag="PLI37_04475"
     CDS             12853..>13812
                     /locus_tag="PLI37_04475"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ58300.1"
                     /translation="MKKKTIYISVVLGIMGLLFSQCNQKEFLSVANPSATDDAFVTSS
                     ISEAFKTLSWCYAEYCKTDAAGGNYNWNDPCSDAEYYPEYNSNNGRIGYLRPAEFTVD
                     GRKAQFNSLYQVLGRCARIGDIIGSKTEYLEAKEAGVTDDWTQLYGEAWTFYCYCYFE
                     LVRHFGDIPFGIENSIVESYGLTSRFEILDKIIAKLKEVEPLMYDLGQGGITAERMSR
                     TFAHQLIAEAALYSGAYQTIRTDSIAQNLYGDIAFTAKYTDNAAKAAYVRRNDYKDYY
                     ELAQTHFRLVLGERKGSLSFITTDERDYCNNPFQRGFQYMADKQ"
BASE COUNT         3784 a         3040 c         3225 g         3763 t
ORIGIN      
        1 aaatagaaat aaaagtatta cctttgcggc cactattttc taattactta aattacaaaa
       61 gaatgtattt gacgaaagaa aaaaaacaag aaatcttcga aaaatacggg aagtctaaca
      121 cggatactgg ctccgccgag tcccagatcg ccttgttttc ataccgtatt tctcatttga
      181 ccgaacattt aaagtcaaat cgtaaagatt atagcactga acgctccctt aagatgttgg
      241 taggcaaacg tcgccgttta ctcgactacc tcaaggaaaa agatattgaa aaatatcgtg
      301 cgttgatcaa agatttgggt atcagacgtt aaaagctgat ccacagcaaa gaccggtctt
      361 gaaagttttg gagtgtttct gacttttggg accggtcttt tttaatacca ctacatcaca
      421 gtttattcca gttaatatcc ttcggtttat tatccattcg accatgacta tccgtttcac
      481 tattcattac cacacttatc cgggacagga gttgcttttt tgcggctctc aggaaacaac
      541 agcctggttg cgcgacgagc aggcaaagcc tatgacctac cggtccggag gatattggtc
      601 gctggatttg cccgtgcggc aaaaaggggt gtttgtttac cgttaccttg tcagggaaaa
      661 tggacgcatc acccgccggg aatgggggcg tcatcaattg acgcttacat ctcttacaga
      721 gcagtttgca gaagttctgg atacctggca gacagaaccg gcgaaatcgg ccggaaccgc
      781 catccccgtc ttttcacttc gttcggacaa ggattggggc ataggtgatt ttggagatct
      841 gttcccgatg attgactggc tggagcaaag cggacaaaaa attttgcagc ttttgcccgt
      901 tcaggacacg accaacagtt tttcctggta cgattcgtat ccttacagca ttatttcggt
      961 ttttgccctg catcccattt acgcagcccc ttctttgttg ccggcacttg aggacagaga
     1021 caaggcagcg tctttcgaag agagagctaa gcagttgaac ttgagcgaaa gtgtgaaata
     1081 cgaagaggtg ttatcgctca aatgggagta tttccggctg ctttttgatc aggaacaagg
     1141 gaaaataatc caatcggttg attataagcg ttttctaaaa gaaaacagcc attggttgcg
     1201 gccctatgcc gcttattgct atttattgcg taagctgaat aatcccgatc ccaaagcctg
     1261 gggggaatat tccaattacg acagcgaaag catttccagg ctttgcgacc ccaggcaatc
     1321 ctggcataaa gacattgcca ttcattatta tatccagttt caactccact gccagctgga
     1381 gcaggtacac gactatgcag ctcaaaaggg catattgctc aagggcgata ttccgatagg
     1441 cgtcaatcga ttcagcgtgg aagcctggca acacaaaaac ttgtttcgcc tcgatttgca
     1501 aaccggcgct ccacccgacg aattttccga tcaggggcag aattggggtt tcccggttta
     1561 cgactggaac gaaatgcatc gttggcaata caaatggtgg cgcaagcgtt tgcaacatat
     1621 ggcaaagtat ttcgatgctt accgtatcga tcatattttg ggttttttcc gtatctggag
     1681 tattcccacc gattcaactt cgggtttatt gggttatttc aagcctgcct tgccctacac
     1741 tccgaaagaa atcagggctt tcggtctgga tttcgaccgg caaacgatgt gcaagccttt
     1801 agttgacgaa gttctgcttg gggaatatcc ggggctggag attttttctt atctcagatc
     1861 gccgggtaat caaaaaagac attcgtcgaa aaaagaatcg cccgctcccg ggtcaaagcc
     1921 cgatcagacg ggtaatcatc aaagatattt gttgaaagaa gaattggaca cccaatccaa
     1981 agtgaggaat tatttcggcg attctactca aaaagaaatt tcccggctgc gggaggcttt
     2041 atgccgtatg tgcaccgaag tcctttttat cgaagacaaa gataaagcag gtcgttacca
     2101 ccccagaatt ctcgggaatc agacagctgc ctatcgttgc ctgagcgaaa aacagcaaaa
     2161 ggccttcgat cgtctttatg agcattttta tttcgaaaga cataacgaat tctggaaaga
     2221 gcaggctatg agaagtttgc ccgaattgct ttcgtcgagc agcatgatcg cctgcggcga
     2281 agatttgggt atgataccgg cctctgtgcc cgaagttatg gagcaactct gtatgctgag
     2341 cctcgaactg gagcgtatgc ccaaaacttt tggcgttgaa tttgaagctc tggatcgtat
     2401 tccctatttg tcggtgtgca caacttccac gcacgatatg tcgccgttaa ggacttggtg
     2461 ggaagaaaat cctttacagg ccggacgcta ctggcaatcg gttttacatg aacaaggcga
     2521 tgcgcctctc gaatgcagtg ccgatctggc ttacaggatt ttgcaacatc acttgtcttc
     2581 caatgccatg tgggtgatac ttccctggca agattggctg gcgctgcata acgatttgag
     2641 aaaaacccgg gcggaaacgg aacgtatcaa cgtgccggag aactcaaaac atcgttggga
     2701 ttacagaata catataaata tcgaggattt tctgaattcc accactctaa acactaccat
     2761 tcgccgaatg attgccgatt ccggcagata attttcaggg tttagtaaat tcaattagta
     2821 aatgcaactg gttgcattgg gatgttgaat ctatgcttgc caattactcc ggcaattcca
     2881 gaatgccatt gaagtggatg tgttggcggt cgggatcctg gaccgagagg ctgcaactgc
     2941 cttcgggaaa ggctgagaga tacaatatat agtttttacc ggtgacgtct tttggcttaa
     3001 tggtaatatc cagcctgccg tctttgcctt cttttacctg ataatcaaaa tcgtgggaat
     3061 tgaatttcac cccgctttct tcgtagctga gcggagcttt ataggctctt ccataatagg
     3121 gaaggtaggc ttctatatta tcaggactta ccttaaggtg gaacccgtaa ctcagattga
     3181 cgcttctcca ggccatggga tgtgccgtct gggcctgaaa aacaaactgc cggttttcga
     3241 tggcggtttt aaaagtctcg gcttctttgg aggtctttgt cgattcggac ccgtcaaaag
     3301 cgtaggcttg attcatacac aaaccacaaa aaagcagaat cggaaacagt gatttaaaga
     3361 ccgtcatgag ggaaaaattt attggtaaaa atacagatta aacgccatat cctctatttt
     3421 ccggacataa aaatgaaaaa cacaaggcta aaaatctaaa acaaaagaag ttttgaaaga
     3481 tttccacaca ataaagagct gatttgtaat acacgagggc gaatttgtat aaaattaacc
     3541 gccggaaaag ggcgttatac aataaagttt aaaaaaaact cttacatttg catgtcttaa
     3601 ccataggaaa aacggtattc acagacctta aacctgaata catatgcgct aaatttttgt
     3661 tataattctt ttctttaatt taaaacctat tttcaaataa ccttaattat caacaatcaa
     3721 aacacgtatt tatgaatctt gtatcatttt tcagacgagt attcgtgacc tcatttctgg
     3781 ccgcgacttg cttgtttgca tttgcgcagg agcaaatggt gagcggtgtt gtcaaagatg
     3841 ccgccggtga accgctgatc ggtgtaaacg tggctacggg agacagaggc actgttaccg
     3901 atcttaacgg tgcttattct ctttcggtgg ctgccgacgg tcagttggtt ttttcctaca
     3961 ttggttattt gtcccagacc atagccatca acggccggtc tgtgatcaac gtaaccatgg
     4021 aagaagatgt cagggaactg gaggcagtcg taaaaatagg ctacggtacg gttcgcaaaa
     4081 gagacctcac aggagcggtg gcctctattc ccggtaaaga aattgctacc attccggtgg
     4141 ctaacgccgc tcaggcacta cagggaaaac tgcccggtgt caatgtggtt tcacaagacg
     4201 gcaggcctga tgccagtatc tccattcgtg tacgcggggg agggtctatt tcacaaagta
     4261 atgagccatt gtatgtagtg gacggttttc cggtgagcag catcagtgat atccctgcag
     4321 aacagatcga aagcattgat gtgctcaagg atgcgtcatc tactgccatt tatggtgctc
     4381 gtggtgccaa cggtgttatt attgtaacca ccaaatctgc caaagaaggc aaaaccagca
     4441 ttacctacag tggttatgca caaagcaagg tgcccactaa atatttcgaa actttgggtg
     4501 cctatgatta cgtagcgtac aactgggctt atgccgacgc cattggagca gcttatcggg
     4561 atgcctggga aatgttgtgg gccatagggc cttacgccgc cacctacggc aatactcccg
     4621 gtatagatta ctacaagaat gtagtggccc aaaactatca gcgtcaggtt tacggcgaaa
     4681 gtttttcgca tagccacaac ctgaacatca ccggtggcaa cgacaaaaca aattttttag
     4741 ttgcgtttaa ccacatcgat gacgatggtc tgaaagtgaa ttcctggtat aaaagaacca
     4801 atgcttctat caagctggat cataaactat acaaaaaact caccttatcg ctcgatacca
     4861 gatttacgca caccaatagc gtaggaaatg aaagcacgac caacacacgg ggttccgttt
     4921 tatcgagttc ttattttttc cgtcccattg tcactcagca tgtcatggga gagttggatc
     4981 ctatggtaaa cagttccctg ggaatgtacg acgaattgct tcaggatgaa ttcaatccgg
     5041 tagccagaat caaagactat actcctgaaa gaaacaatcg cagccttagg gccaatacgg
     5101 ctttaaactg ggaaattgtc aaaggtttgg ttgcacgcac cgaattaggt ttgaatacct
     5161 attggaacaa aaatcatacc tggacggggg ctatttttaa cgactattac gatgccaacg
     5221 gcaacaaaac gtacagcggt aatgccagca tttcccgttc cgaaggttgg aatatgcgtt
     5281 gggccaacgt cctgagttac gaaattcggg atttgggaga agctcatcat gtaggtgtga
     5341 cgctgggtca ggaaataatg gattctaact cggaaagcat gtctatttcg ggcagccgct
     5401 atccggcctc ttatactccg gaacgcgcct ttgctatgat ggatcagtac gatcccggta
     5461 acctggctta tcattctctg tccggatcga taggaacgcc ttcaagattg atgtcctatt
     5521 ttgccagagc taattacggc ctgctcgacc gctacctgct cacagtaact ttccgtgcgg
     5581 atggttcttc ccgttttgct cccaccaatc gctgggggta tttccctgcc ggagccattg
     5641 cctggcgtgc aagtgaagag aattttatca aagagcagga ctgggtagac aacctaaaac
     5701 tgcgtttttc cctgggctct gtaggtaacg acggaatcag tgcggatctt tggaaaatga
     5761 actggactac gggaggattg gcaaactatt ctattaacga agaaaggcag gtaatctatc
     5821 agccaggttc cgatgttatg gccaacccca acctcaaatg ggaaacgacc attacccggg
     5881 accttggttt ggattttggt ttcctggata acagagtgtt cgggtcattg gatctgtatt
     5941 acaataccac caaagatctg ttgatgctga ctcccatttc ggatatcacc ggtttttcct
     6001 tcacctacga taatgtcggg agtaccagca acagaggtat tgaagtactt ctcggtggag
     6061 atatcataaa aacaaacgac ttcaacctta ccgccagttt aaatgtgaat attaaccggg
     6121 gcagggtaga agaactggcc gaaggtatcg acggacttta taagaccgaa tggggttcca
     6181 ccatgaccag acctaatacc ggagattata ttttggaaga aggccaaccg gttggtatgg
     6241 tccgtggtta tacctacgaa ggttggtata ccaccagtga ctttgactac gatccggtca
     6301 caggtattta cacccttaag gatggcgtgc ccgatatagc agccggcgta ataggaactg
     6361 tttacggaac cgaaaacaat aaacccggat cccagactgc ctatcccgga gttattaagt
     6421 acgctgacat cagcggcccc gatggcgtgc ccgatggtgt ggtcgacgaa agcgacgtgg
     6481 tgattatagg taacatgaat cccaaacaca ccggaggtct caacctcaat gcctcttaca
     6541 aaagttttga tttgggattg ttcttcaact ggagttacgg caatcaaatt tacaatgcca
     6601 attacctggc taccatatac ggaagcaaaa acgacggttt atatcgtaac agaatggatt
     6661 atttagccgg tgcctataag atttacgaca tcaaaaacgg aaatctggta aaagtaaccg
     6721 atccggcagc tctggatgct ttgaataccg acgctagtct tttccttccc tatcacgaaa
     6781 acgcggttgt ttctacgctc ggaatagaag atggttcctt cctgcgtctg aacacagcta
     6841 ctttgggtta taccatgcct aaaaatatga tcagaaagat aggtttggtt aatgctcgcc
     6901 tgtatgccag tgtgtataat ttattcactc tgacaaaata ttcgggcctg gatcctgaag
     6961 tcaacaccaa taccagccag ggtggagcac gttatcctac caccggtttg gattgggggg
     7021 cttatcctcg tgcacgctcc ttcacttttg gagtcaatct ggagttttaa ttattaatgc
     7081 cgtataacaa tgaaaaaata tatttatatc cttttaggtg tctttttgct gactataggc
     7141 actacttcgt gtggagagga ttttttggag gtatcttctc cttcgcaggt ggacgaagac
     7201 tttgtatttt cgacgcctga tgaagcctat aaagttctga tgggttgtta cgaaattttt
     7261 cgtggtagat cctatgttca ctccaatgga cttttttacg acctgataat cggcggttcc
     7321 gattcggaat gtcacccgga gtcgtattcc gcacaacaga ggcatgtgcc cgaaggtttg
     7381 tatgccgtcg aagacttttc catcgatttc agcaattttc aaagagcttg gaggtattgc
     7441 tatcaaatgg ctaaccgcct ggcaatagta attgcgcaaa ttgaaactaa gccggctttc
     7501 caggcagcct gggatgccca ggaagtttcc gaatggaccc agctttatgg agaagcagtc
     7561 gctttgcgtg ccattgccta tcatgaactg attcgttttt ttggcgacat cccgtatttt
     7621 gacataccca tctacaccca ggatcaatcg gatgtgggct gtacatcaag gtttgttatc
     7681 tacgatgatt tattggacga tctcaaacgg gttgagcctt ttatgtacag attgggagaa
     7741 gcgggtgtaa atgctgaacg cattaccgga actttcgtac aggggcttat cggaagaatt
     7801 gccctttatg ccggaggtta tcaactctac cgcaccgatt tcgattatgg gataactctg
     7861 gagagacacc ccaatattaa tggagagaaa tggaatgcta tctacgcccg tcatccccaa
     7921 taccttgatt attacaggat tgctgaacaa tatctcgggg aatgtgtaag cgaaggcaaa
     7981 gctactttga taactacaga tgagcgtggt tatggcaatc ctttccaacg caatttccag
     8041 tatatgatgg atctgcaggt aagtcccgaa tccttgtttg agatagccga aacccaaggc
     8101 ttccagaccg aacgccccta tgctttcggg cgtccctcca aaggaggcgg atccaacaac
     8161 ttcccctgca aggcgtatgg acagagtcgt atttatgcct cgttctacta tggtgatttc
     8221 gatcctttgg atttgcgtcg tgatgccagc gtgaccgtta cctgcaacca gcatccttgt
     8281 accgaagtgt tgttgttgtt cgatcccggt tcgcagaaca agggaggttt ggccaacaat
     8341 aagtgggacg aaagccgtat gtccaatccc tatactacca ggcaacgtca atcgggtatc
     8401 aatacctgtt atatgcgtat ggccgatgta atcctgatgc tggcggaagt gtatgcggaa
     8461 ttgggcaaag aaggcccggc caaaacagaa ctttccaaag tcagggcaag agctttcccc
     8521 gcaacagcca aagccaccaa ggtggatgct tatattgctg ctttaagcgg tgatgcactc
     8581 aaagaagcca tcgctcagga acgtaaactt gagttcgccg gcgaaggatt gcgtcgctat
     8641 gacttgattc gtaccggcaa attgcccgaa aaaatagttg atctccgtaa agctcaggga
     8701 gccatgatca atgggttgaa aaccaatggt tattatacct ttgccaacgg caataccata
     8761 tctgcttata tttataccaa gcctgtaaac accgccgatc ttgggatgac ttatatgctg
     8821 accaagcaat gcgatgtctt gccgggagat cccgcatatc cggtaaaatt cccgagttgg
     8881 agaggccaac acgatggctg ggaaggtttc gataatacct ccggcactcg taatctggcc
     8941 attcaaggtt tgtttgaatt catcgattca gcaagcgtac aaggcaaagc ccttatcgct
     9001 gccggttatg aagtggttcc ctggggcagt accattgttg ccaacgaaga ccagtacctg
     9061 cacgatatct tcaagggata taccgatgcc gactttacca gcaaaacacc tccccgttat
     9121 ctctgtccga tgtcgtcgac cacgatatct aaatccaatg gtaaaattac caacgggtat
     9181 ggtttttcac aggaataaca attggtgatt tttgcttgct tattaggtta atatcctatg
     9241 actcaagggc gggattcatg tacatgaatc ccgctttttt tacacaccaa ccagcagata
     9301 acgggtgtaa aattgtgtaa tattaactat cacggtgggg acgttaggcg ataatgtaaa
     9361 aaaaatcttt tacatttgtg tgccttaacc atggaaaaaa cgatatatac aaaccttaaa
     9421 ctctatacct atgtactaaa ctttgataga aatctctttt taatttaatc ttattttcaa
     9481 ataaccttaa ttatcaacaa tcaaaacacg tatttatgaa tcttgtatca tttttcagac
     9541 gagtattcgt gacctcattt ctggccgcga cttgcttgtt tgcatttgcg caggagcaaa
     9601 tggtgagcgg tgttgtcaaa gatgccgccg gtgaaccgct gatcggtgta aacgtggcta
     9661 cgggagacag aggcactgtt accgatctta acggtgctta ttctctttcg gtggctgccg
     9721 acggtcagtt ggttttttcc tacattggtt atttgtccca gaccatagcc atcaacggcc
     9781 ggtctgtgat caacgtaacc atggaagaag atgtcaggga actggaggca gtcgtaaaaa
     9841 taggctacgg tacggttcgc aaaagagacc tcacaggagc ggtggcctct atctctgcaa
     9901 aacaaattgc taccattcct gttcccaatg tggctcaggc attacaggga aaaatggccg
     9961 gtgtcaatgt gattacgcaa gatggccgtc cggatgcttc tatttccatt cgcgttcggg
    10021 gcggcggctc gatttcacag tcgaacgaac ctcttgtttt aatcgatggt gttccgggaa
    10081 cccttagtga tgttccttcg gatcaggttg aaagtgttga tgtgctcaaa gacgccgcat
    10141 ctactgctat ttacggagct cgcggtgcca acggtgttgt tttggtaacc accaaagggg
    10201 ctaaaagcgg aaaaacagtt gtgtcgtaca acggttatgc caagttcaat caacccacca
    10261 agtatctgga aaccctgagt ccatacgatt atgtctctta tgtgtgggcc aatgccgatg
    10321 caaacggcat taactatgta gactctttta ccaaactctt cggaataggc gcctacggag
    10381 atattgaaag atatcgcaat gtagaagcct acgatgttca gaaaaaagtt tataacagtt
    10441 cgttttcaca caatcatgat ttgacggtga gcggaggtac cgatttaact aaaatcattt
    10501 tgggagtcaa ctataacgac gaacaaggta tgaagattaa ttcgttccat aagcgggcaa
    10561 gcgtttcttt gaaagtagat caaaagatca acgagaaaat ggatcttggt ctggatacgc
    10621 gttatgtaga cataaccagt atgagcgatg agggtacgac ctccggatcg ggttccatct
    10681 tatcctatgc tttccgtttc cgtccgatcg ccaccggtga tattttagga gacttaaacg
    10741 ctttgaacga aggagccgtg gaacagtacg gaaaattctg cctttgggac agatacgatc
    10801 cttacaaccg gataagagat tatgaacccc ttagaaagcg tcagactctt cgcaccatcg
    10861 ggtcctttaa ttgggaactt ttcaaaggcc tggattatca caccgatttg acgctgcaac
    10921 gctcctggaa tcagaataaa ttctggggag gccccgttca taacagttat ttggacgatg
    10981 ctacgggaga ggttctttat gccggtgcgg ctactttata taaagccgat agctggtcgt
    11041 atcgctggtc gaacaccctg aattacgaaa ttgaaatcaa taaggcaaat caattgagcg
    11101 tgttgttggg ccaggaagtc tccaattcgg gaggaaacgg aatgactatt acagccgatt
    11161 tcttccctgc caattacacg aaagaaaatg ctttcgcaat gattaaccaa tacgataaag
    11221 cggccagtac caaccagagt cctttttcat ccagtttttc gactccttca cgccttctgt
    11281 cgtattttac ccgaatgaat tatagtctgc tcgatcgtta tctttttgca tttaccttca
    11341 gagcggacgg ttcttcgaaa ttttcaccga atcatcgttg gggttatttc ccggcaggag
    11401 ctctcggatg gcgtttgagc gaggagtctt ttttaaagga cgaagactgg ctggataacc
    11461 tgaaacttcg tgcctcttac ggctctgtcg gtaacgacgg gatcagcgcc gatttatggt
    11521 cgcaaaattg gacggcagaa accgatgatc gttggtatta ttctatagat ggcgttgctc
    11581 agccttctta cgatttggcc tcctctcaga tggccaatcc cgatttgaag tgggaaacaa
    11641 cgattaccag aaacgccggt attgatttcg gcgtattcgg aaatcgcctg acgggaacct
    11701 tggattttta ttggaataca accaaggacc ttttgatgaa caccgtgctt cccggtatca
    11761 caagtttcag ctcgaccttc gccaatatag gccaaacatc gaataagggt gttgaattct
    11821 ctttgaacgg cgtattgttc gaaaacaacg actggaatgt tgaagcaggc ttcaatatca
    11881 atttcaacag gaataaagtg gacgaactgg ctaatgccca ggagctgatc gacggcaaag
    11941 gcttgtatta cggaaccggt tggttttctt ccggatatcc ggggaacgac tttggtttgt
    12001 acgagggcaa acccgttggt ttagtacgtg gtctcgttta cgaagggatg tattcgttgg
    12061 atgattttga ctataatacc gcagaaggat tgtactacct gaaagacgga gtccccgata
    12121 tcagccccaa tattaccggt gttatgcatg ggatagctac tcccacaggg caaaatgcct
    12181 atccgggtat ggctaaatat agcgatacgg acggaagtgg tatggttgac ggagaggatt
    12241 ttgtcattat aggagacatg aacgccaagc ataccggtgg ttttcatatc acagcccaat
    12301 acaagcaatt cgatttgggc ctgtatttca actggagtta cggcaatcaa gtgtataacg
    12361 tcaacagaat ggcttctttg atgggttata aggaaacagg tgtttatcaa aacaagctgg
    12421 cggtcttggc ttcgagttat aaaatctacg atatagtcaa cggacaattg gttagataca
    12481 acactcccga agaattggca gccttgaata aaaatgctga atatccgttg tgttataatg
    12541 aatcgggtgt agtatcgtcg ctgggtattg aggacggttc ttatctcaga ttgaatacgc
    12601 tcaatttcgg ttatactttg ccaaagagcg tatcgactaa gcttaaaatc aataatatgc
    12661 gcttgttttt cacggcttac aacctattga cactgaccaa ataccaaggt cttgatccgg
    12721 aagttaacgc aaatgagcgt gttaatcacg ccgtttaccc gacaccagga ctcgactggg
    12781 gtacctatcc ccgtccgcgt tccttagttg ttggtgcaaa catttctttt taactaataa
    12841 acacaaacaa ctatgaaaaa gaaaactatt tatatttccg ttgttttggg aataatgggc
    12901 ctgctctttt ctcaatgcaa tcagaaagaa tttttatcgg tagcaaatcc ttcggctacg
    12961 gacgatgcgt ttgttacttc ttccatttct gaagcgttta aaactttatc ctggtgttat
    13021 gccgaatatt gcaaaacgga tgccgcgggc ggcaactata attggaacga tccctgctcg
    13081 gatgcagagt attatcccga atacaactcc aataacggcc ggatcggtta tttgcgtccg
    13141 gctgaattta ctgtagatgg ccgtaaagcc cagttcaaca gcctttatca ggtcctgggc
    13201 cgttgcgccc gcattggcga tatcataggt tctaaaaccg aatatctgga ggctaaagag
    13261 gccggtgtta cggacgattg gactcaattg tacggcgaag catggacttt ttattgttac
    13321 tgctattttg aactggttcg ccatttcgga gacattccgt ttggtatcga aaattcaatt
    13381 gtcgaaagtt acggtctgac ctctcgtttt gagatattgg acaagatcat tgccaaactc
    13441 aaagaggttg agcctttgat gtacgacctg ggtcagggag gcatcacagc cgaacgtatg
    13501 tccaggactt ttgctcatca attaatagcg gaagcggctc tgtattcggg agcatatcaa
    13561 accattcgta ccgattcgat tgctcaaaac ctgtatggtg atatagcctt caccgccaaa
    13621 tacaccgaca atgctgccaa agccgcttac gtccgtcgta acgattacaa agactattac
    13681 gagcttgctc agacacattt ccgccttgtt ttgggagaaa gaaagggtag tttgtctttc
    13741 atcacaacag acgaaagaga ctattgcaac aatccgttcc aaagaggttt ccaatatatg
    13801 gcggataaac aa
//
LOCUS       DAOSTD010000046        13679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_4571_length_13679_cov_14.998018, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000046 DAOSTD010000000
VERSION     DAOSTD010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13679
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4571_length_13679_cov_14.998018"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PLI37_04480"
     CDS             complement(<1..515)
                     /locus_tag="PLI37_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58301.1"
                     /translation="MNKPDDFSIPRMEKQTFPNGMDFYHRKTDAEGLLKLDLILPAGI
                     QYQKRPLLARLTNALLKEGSRGMTSAQIAERFDYYGAFILLGCGLENAYVNLYTPKKF
                     FSFTLKLLNELLRYPSFPQKELDISLEKNLQLHLIELQKVQVLAGLEINKCLFGPEHP
                     YAKQLQEEDFD"
     gene            complement(512..>1090)
                     /gene="kdsB"
                     /locus_tag="PLI37_04485"
     CDS             complement(512..>1090)
                     /gene="kdsB"
                     /locus_tag="PLI37_04485"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOQ58302.1"
                     /translation="AVEAFGGKVIMTSSAHRSGTDRCAEASSRLGGDFEVVINIQGDE
                     PFIQGEQIQALKACFNKPETEIATLVKAFKKEDGLQALLNPNSPKVVLNSRGEAMYFS
                     RSVIPYLRNFPQSQWLEKNVFYKHIGIYAYRRTVLAELARLEASHLEQQESLEQLRWL
                     ENGYKIGTALTQVENQAIDTPEDLERLLKNLR"
     assembly_gap    1091..1190
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1191..2365)
                     /locus_tag="PLI37_04490"
     CDS             complement(<1191..2365)
                     /locus_tag="PLI37_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOQ58303.1"
                     /translation="MKAGNYLLLSFLLFGLLSLNAEDSLAVRKLVYQVDLRDNVNSFT
                     WMQVQKGFAQAEAEEASLILLDMNTYGGEVVYADSIRTRILNTETPVCVFINNNAASA
                     GALICIACDKIFMRSGAVIGASTVVDQSGEVAPDKYQSYMRGIIRATAEAQGKDTLIA
                     GGDTMYVWKRDPAIAEAMVDPVVEIPGVIEKGKVLTFTATEALQNKYCDAIVQSKEEI
                     LETHYGYGDYELKVFRASLKDKISGRLMGTALRAILIMIIVAGIWFELQSPGIGFPSV
                     AAGIAAVLYFAPLYLDGLVQYWEIILFIAGVILIFLEIFVIPGFGIAGILGILAVVLG
                     LSFALVDNTGLSFDFEGLDVLGEALLLVILSMLIAFIISLWLTSKIGVGKGVFRHLAL
                     "
     gene            complement(2438..4219)
                     /locus_tag="PLI37_04495"
                     /pseudo
     CDS             complement(2438..4219)
                     /locus_tag="PLI37_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145481.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
     gene            complement(4395..5168)
                     /locus_tag="PLI37_04500"
     CDS             complement(4395..5168)
                     /locus_tag="PLI37_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOQ58304.1"
                     /translation="MKILILGAGKMGSFFADVLSFEHETAVYDIDPRKLRFIYNTCRF
                     KELEEIREFAPELLINAVTVKYTIEAFKTVLPLLPASCIISDIASVKNDLEDFYAGCG
                     HRFVSTHPMFGPTFASLNNLSSENAIVITESDHLGKTFFLDLYRKLGLNIFEYSFKEH
                     DKTVAYSLSIPFVSTLVFSAVMKHQDAPGTTFKKHLAIAHGLMNEDDFLLQEILFNSE
                     TPAQLELIRSELKQILEIVKNRDAQRMKSYLEKIRNNIR"
     gene            complement(5165..6253)
                     /locus_tag="PLI37_04505"
     CDS             complement(5165..6253)
                     /locus_tag="PLI37_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOQ58305.1"
                     /translation="MQFNRQLFPDLDLTRPIVISGPCSVETEEQVLTTARQLAEKGIR
                     IFRAGIWKPRTRPGAFEGIGTKGLAWLKLVKEQTGMYTCTEVASTQHVYEAVKYGVDM
                     LWIGARSTVNPFLMQEISDAVQGLDIPVLVKNPVNPDLELWVGAIERLYKAGIRKIGV
                     VHRGFSSWDKQIYRNAPHWHIPIELRRRFPDLLMFCDPSHMGGKRELISPLSQQAMDL
                     NYDGLFIETHYKPETALSDSGQQITPDVLQYILKTLVVRQGGKPDAEKLSAYRAEIDE
                     YDDQLVELISKRMKISKLIGHYKKENNIQVLQAARYNEIIEERVKQAAGLGIKSDCMQ
                     KILEAIHEESVRLQIEIMNMDNSKTSEK"
     gene            complement(6281..7492)
                     /locus_tag="PLI37_04510"
     CDS             complement(6281..7492)
                     /locus_tag="PLI37_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ58306.1"
                     /translation="MQKEIKPAKRLEGINEYYFSSKLREVAMLNAQGKDIISLAIGSP
                     DLMPRAEVVETLCKEAAQKTAHGYQPNVGRKELRSAFAAWYKEWYGVDLDPDREILPL
                     IGSKEGVMHVSMTFLNPGDAVLLPNPGYPTYRAVCKLLQAEVLEYELDEKNGWQPDFE
                     ALEKMDLSRVKLMWVNYPHMPTGAPAQKSTFEKLVDFGRRHGIVVCNDNPYSFILNNH
                     PLSILSVPGAKDCCLELNSLSKSHNMPGWRIGMLAGNPEFVSWVVRLKSNVDSGQFLP
                     MQLAAVKALQSERSWYKELNRRYSSRRVLIEELMCSLHCTFDAGQEGIFLWGRIPDDY
                     TDAAELCDELLYKARVFVAPGFIFGSKGNRYIRISLCSPEEQIREARRRVCNYLHKQK
                     CSSAGKPVLTS"
     gene            complement(7728..9632)
                     /locus_tag="PLI37_04515"
     CDS             complement(7728..9632)
                     /locus_tag="PLI37_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOQ58307.1"
                     /translation="MTIYHDILKQYWGYEDFRELQLEIIESVAAGKDTLGLMPTGGGK
                     SLTFQVPALAMEGICLVITPLIALMRDQVERLKSMDIRAACLHSGMTRQEIQIVLDNC
                     LYGDFKFLYVSPERLGSDLFLQRALGLPVNLLVVDEAHCISQWGYDFRPSYLNIARVR
                     EYFPSIPVLALTATATPEVVEDIQDKLHFAAPNVFKVSFERKNLAYVVRETQDKDKAL
                     LQVLKGVDGTSIVYVRNRAKTKQIANFLKGEGVEAAHFHAGLNDQTKQERQAAWKNGT
                     CRVIVATNAFGMGIDKPDVRTVIHMDLPDSLEAYFQEAGRAGRDGQKSYAVLLYHKSD
                     STLLKKRLSDNFPNKKYIRKVYEALGNYYQLAVGSGQGMNFPFDLQDFCGKFHLNMLQ
                     AYNGLKILELAGYLELTDEQDSTSRLYFLSSREDLYHLPRSGNKTDRVLQVLLRSYTG
                     LFSDFVRIQEEVLASRLQMTGEEVYESLLYLSRQGVIHYIPRKKTPFICYTQSREESE
                     RLHIGPEVYEERKKRYVERIQAMQAYAAEDQPCRSKRLLQYFGQKRVSNCRQCDVCLR
                     KQQEGLRQYEFEDISRDMLAALENSNPDLEELIDSLPYDSNKSLKVFRFLCDRGDIVQ
                     REHRIFRKTD"
     gene            complement(9707..11428)
                     /gene="recJ"
                     /locus_tag="PLI37_04520"
     CDS             complement(9707..11428)
                     /gene="recJ"
                     /locus_tag="PLI37_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOQ58308.1"
                     /translation="MSKWNLLTLTPEQETQKEQLAEELGISPILAKLLILRGISSFSE
                     AKKFFRPQLSDLHDPFLMKDMDKAIARLNLAIANKEKILVYGDYDVDGATAVALVYKF
                     LRQYSLNIEFYLPDRYVEGYGISYQGIDYAFEHQVKLIIVLDCGIKAVEKVAYAKDKG
                     IDIIICDHHKPDDVLPDAVAVLDAKRPDCQYPYKELSGCGVGFKFMQAFAQNNGIPFD
                     RLLPLLDLVVVSIASDIVPITGENRILAFHGLKQLNTKPSVGLKAIIQLSGLVGKEIT
                     ISDIVFKIGPRLNASGRIQSAGEAVELLVTKNLRFAKEKSLQIDQYNQTRKDLDRNIT
                     EEANAALKACTNLDDRKGIVIYNEEWHKGVIGIVASRLSEEYYRPAVVLTRSDDLATG
                     SARSVPGFDIYKAIDSCRDLLENFGGHTYAAGLSMKMEKVEEFTRRFEQYVADTISLE
                     QLTPTIDIDAEIKFQEITPKFFRVLKQFNPFGPDNNKPVFSTRRVFDYGTSRLVGRDQ
                     EHLKLELVDSHSENIMNGIAFGMHAHNDHVKSFNPFDICYNIEENYYNGNSSIQLLVR
                     DIKVVED"
     gene            11637..12209
                     /locus_tag="PLI37_04525"
     CDS             11637..12209
                     /locus_tag="PLI37_04525"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOQ58309.1"
                     /translation="MSLQEEINKAGEVLKQGGVILYPTDTVWGLGCDAGNPQAIEKIH
                     AIKKRAENKAMIVLLDNPYKLNLYVEEVPELAWNLIELSDKPLTIIYRGAKNLAANLI
                     AEDGSVAIRITREQFSQSLCQSFRKAIVSTSANISGTPAPGSFRDISAEIISAVDYVV
                     DYRRNEPAGKAKPSSIISLGNKGEIKIIRP"
     gene            12232..13647
                     /locus_tag="PLI37_04530"
     CDS             12232..13647
                     /locus_tag="PLI37_04530"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOQ58310.1"
                     /translation="MEKDKKYILRYALLDYIATLIAWQLFNIYRFYEVRSTVYFNTLG
                     DFLINTKAILMGVGMPLFWLIVYFYSGYYAKPRQKNHTSDLLSTLFSTLVGSLIIFFA
                     VIINDYPEQYTLYYRVFAGLFLIHFFCTWFVRFLQTSRLLNLHAEGKVGINVLIIGSG
                     KVAGKLSRQMQQTKNSSAYLLKGFIRVNSEEKDVAEQNILGDKEQLKEIIKEQRIEAL
                     ILATDRQDSDYTEGLLKQLYVYHLDILSVIHKQDLLFSNITLYSLPGVPLKYLSPVQL
                     KPWEQNLKRSTDILVSVLGLLVLSPLFVFLALRIRLESAGPILYRQERLGKEMKPFTI
                     LKFRTMFAHSEPEGPLLSSLNDERITPFGRFLRKYRLDELPQLWNVLKGEMSLVGPRP
                     ERRFYVERIVDQAPQYYLVYRVKPGITSLGMVKYGYADSVEKMIERLEYDLIYLKNQS
                     LLMDLKILIFTFKPIALGKGI"
BASE COUNT         3576 a         3254 c         3025 g         3724 t
ORIGIN      
        1 ctgtcgaaat cttcctcctg caattgtttt gcgtagggat gttccggccc gaacaaacat
       61 ttatttattt ccaaaccggc cagtacctgc actttttgca attctatcag atgcagttga
      121 aggttttttt ccaggctgat gtccaattcc ttctggggga atgaaggata acgcaggagt
      181 tcgttgagca gtttcaaggt gaaactgaaa aacttttttg gtgtataaag attgacgtaa
      241 gcattttcca aaccgcaacc caaaaggata aaagcaccgt aataatcaaa gcgctctgca
      301 atctgagccg aagtcatacc gcggctgccc tctttgagaa gagcatttgt caacctggcc
      361 agcagaggac gcttttggta ttgtatgccc gcaggtaaaa tcaaatcgag tttaagcaaa
      421 ccttcggcat cggttttcct atggtaaaag tccatcccgt tcggaaaagt ctgtttttcc
      481 atgcgcggga tgctaaagtc gtccggcttg ttcatcttag atttttcaat aagcgctcca
      541 aatcttcggg cgtatctatg gcctggtttt cgacctgagt cagggctgta ccgattttgt
      601 atccgttttc gagccaacgc aactgttcca gcgattcctg ttgctccagg tgagaggctt
      661 cgagcctggc cagttcggcc aaaacagttc ttctataggc ataaatgcct atgtgtttgt
      721 aaaagacatt cttttcaagc cattgacttt ggggaaaatt ccgaaggtaa gggataaccg
      781 aacggctgaa atacatagcc tctccccggg aattcagaac caccttgggt gaattgggat
      841 tcaggagagc ctgcaaacca tcttcttttt taaaagcctt gaccaaagtg gctatttcgg
      901 tttcgggctt gttgaaacag gcctttaagg cttgtatttg ttccccttga ataaaaggtt
      961 catcgccctg tatgtttatt accacctcaa aatcacctcc cagcctggac gaggcttccg
     1021 cacaacggtc ggtgccgctg cgatgagcag aagaagtcat aatgacttta ccgccaaaag
     1081 cctccacggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agggccagat
     1201 gcctgaaaac acctttgccc acaccgattt tggaggtcaa ccaaagcgaa ataataaaag
     1261 caatcagcat gctgagtatt accagcagca aggcttcgcc caagacatcc agcccttcga
     1321 aatcaaaact caggcctgtg ttgtcgacca gggcaaaact caaacccaac accactgcca
     1381 atatgcctaa aatgccggct ataccgaaac cgggaatcac aaagatttcc aaaaaaatca
     1441 gtatcactcc ggctataaac aatataattt cccaatactg caccagaccg tccaggtaca
     1501 ggggggcaaa atataaaaca gcagcgattc cggccgcaac gctgggaaaa cctataccgg
     1561 gcgattgcag ctcaaaccag atgcccgcca caatgatcat aatgagtatt gcccgcagag
     1621 cagtccccat cagacgaccg gatattttgt ctttgagact ggctcgaaac actttcagtt
     1681 cgtaatcgcc gtagccgtaa tgtgtctcca aaatttcctc tttgctttga actatggcgt
     1741 cgcaatactt attttgcaag gcttcggtag ccgtaaaagt aagtaccttg cctttctcga
     1801 tcacaccggg aatttccacg acgggatcta ccatagcctc ggcaatcgcc ggatcgcgtt
     1861 tccatacgta catggtatcg ccgccggcta taagagtgtc cttaccctga gcttcggctg
     1921 ttgccctgat gatgccgcgc atgtaggact gatatttgtc cggtgccact tctccgctct
     1981 gatcgactac cgtcgaagcc cctataacgg ctccgctgcg cataaagatt ttatcgcaag
     2041 caatgcagat cagagctccg gccgatgcgg cgttgttgtt gataaataca cataccggag
     2101 tttcggtatt taggatacga gtacgtatgg aatcggcata tacaacttct ccaccataag
     2161 tattcatatc cagcagaatc agagacgcct cttcggcctc ggcctgagcg aaaccttttt
     2221 gtacctgcat ccaggtaaag ctgttgacat tatcgcgtaa atcgacttga taaaccagtt
     2281 tacgaacggc aaggctgtct tctgcattta aactaagcag gccaaaaagc agaaagctca
     2341 gtaaaagata attcccggct ttcataagaa tttagaaata gccactctcg tcgggattgg
     2401 cgtttttgtt gcgttccagt gtttcttcgt cggaaagcaa cttatccagg ataggagtcg
     2461 tgattccgct aataacaggc tcgagaggac tctgcaagtc gagcactata atttcgttct
     2521 gccctttgtt gagccaacaa cccggcacaa acaaggtctg ctgaggccct atttcccaga
     2581 agcggcctat ggcgtatccg ttgatccata ccagaccttt gctccaattt tgcatatcga
     2641 tgtaaacatc gccggttttg tccagattga acgaaccttt gtaataggcg ggcatgtgac
     2701 agtcttgaga cctctcaaca aattgcaacc tgccgctcaa agcgtcttca acaggaaaac
     2761 ggtacacctg gaagcctgta agttcttttt ctttccggcc ttcctgcacc gaaactcttt
     2821 gcgtaatgcc tttgtagtcg tggatggttt tagaataatt cacccggccc atattctcga
     2881 ccagaatgtc caatacggct tccttttcgg ttgccggcaa ttccagtatt ttttcggctt
     2941 tgcggcgatc gagagtgccc aggcgcttac cgtccacaaa aacaatagcc cagtcgtgcg
     3001 cctcttccac tatgagttta cggcctgcag ctcctgccgg caaggtagtt ctgtataata
     3061 tgctgccata gccctgatcg aaaaactcca ttgaacgtac ctgatccgac aggatgggct
     3121 cgggcagatt ttggaataag ggcgctatct gattcagttc aaacggcgga atttctatca
     3181 tcggtaaagc tttgggcact ttgggcaaag ctttgtcgct gtattttgcc aacaattcac
     3241 gtacggcata aaatttctcg gtggtctgtc cggcttcgtt gatgggtgca tcgtaatcgt
     3301 aggaactgca ccacgaagcg taaggaggtg aattggcacc cgtccagtgg ccgaaggtgc
     3361 ttcctccgtg agtcatgtag agactgaagg aaatgtcttt ttcgagcatt tcctgcatac
     3421 ctttaaccaa ttcttcggca ctgcgggttt cgtgcgggct gccccagcgg tcgaaccagc
     3481 ccgaccagaa ttcgctgcac ataagcggcg tatttggacg caggcgtttc agctcacgaa
     3541 attgattgtc tatgttggca cctgtgccga aattgatggt ccacaacaaa tcgtccaggg
     3601 cattgttttg gaaattggaa ttccagtcgc attgaaacaa gggcacatcg gtaaagccgg
     3661 cagcccttac tatatcgcgg atatcctcca cataaggttt gtcggtaccg taggagccga
     3721 actcgttttc tacctgtacc atgatgatat taccacccct gttgatttgg agaccggcca
     3781 attggcggcc cacttcgttc atgaagattt ccactctttc caaataatag ggatcttgcg
     3841 aacgcagggc tatgccttct ttcttgagca gccaccaagg caaacctccc atctcccact
     3901 ctgcacaaac atagggtccg ggcctgataa tgacgtacat accttctttt tgcgccatac
     3961 ggcaaaattc agccacatcg ttttgccctt cgaaatcgaa aactccgggc tcttgttcgt
     4021 gtatattcca aaaaatatac aggcatatag agttcatgcc taaggctttg ctcattttga
     4081 tgcgatgttc ccagtaaggc ctggggatac gggtgtagtg catttcggca gccttaatca
     4141 caaaggcttc tccattgagc aggaattgct tgtctcctat ttcgaagctc gtggctttgg
     4201 gagcattaca tcctgtcatc aggccaatca ggcatagaat gattgttttc gttaaaagct
     4261 gtctgttcat tgttctcttt ttaatagtaa cacaaaacgc caccggtctg cccaaactca
     4321 ggaataagcc aaggcgacca atggcgcgaa aatacaaaaa atagtaagta cctaaaaatt
     4381 tgaccagggg aaacttaacg gatattgttg cgtatctttt ccaaatagct tttcatcctc
     4441 tgagcatctc tgtttttgac tatttccaga atttgtttca gttcggaacg gatcaactca
     4501 agctgggcgg gagtttcgga gttgaagagt atttcctgca aaagaaagtc gtcttcgttc
     4561 atcaggccgt gggctatggc taagtgcttt ttgaaggtag ttcccggagc atcctggtgt
     4621 ttcatgaccg ccgaaaaaac cagtgttgat acaaagggaa tagacagaga gtaagcaacg
     4681 gttttgtcgt gctctttgaa ggaatactca aaaatattca atccgagctt gcggtacaaa
     4741 tccaggaaaa aggtcttgcc caggtgatcg ctttcggtaa tgacgatggc attttcgctc
     4801 gaaagattgt tcaggctggc aaaggtcggc ccaaacatgg ggtgggtcga tacaaagcga
     4861 tgaccgcaac cggcataaaa gtcttccagg tcgtttttga cactggcaat atcgctgatg
     4921 atgcaagaag cgggcaacaa aggcaaaacg gtcttgaaag cctcgatggt gtatttcacc
     4981 gttacggcat ttatcagcaa ttccggggca aattcccgga tttcttcgag ttctttgaag
     5041 cggcaggtat tgtaaataaa acggagtttc cgcggatcta tgtcgtaaac ggccgtttcg
     5101 tgctcaaaac taaggacgtc ggcaaagaaa ctgcccattt tgccggcacc caatattaga
     5161 attttcattt ttccgaagtt tttgaattgt ccatattcat gatttcaatc tgcagcctga
     5221 ccgattcctc gtgtatggct tcgagtattt tttgcataca atcgctttta atacccaaac
     5281 cggcggcctg tttcactctc tcttcgataa tttcgttgta acgggcggct tgcaatacct
     5341 gtatattgtt ttcttttttg taatggccta tcagtttgga tatcttcatg cgtttgctga
     5401 taagttctac cagttgatcg tcgtattcgt cgatttcggc ccgataggca gacaactttt
     5461 ctgcgtcggg tttgcctccc tgtcgtacga ccagtgtttt cagaatgtat tgcaacacat
     5521 cgggggtaat ttgctgaccg gaatcactca atgccgtttc gggtttgtag tgcgtttcaa
     5581 taaacaaacc gtcgtaattc aggtccatgg cctgctgact caatggagag atcaattccc
     5641 gcttgcctcc catgtggctg ggatcgcaaa acatcagcaa atcggggaaa cgccggcgca
     5701 attctatggg aatatgccaa tgtggggcat tgcggtagat ttgtttatcc caggaactga
     5761 agcctctgtg aacaactccg atcttgcgta ttcctgcttt gtaaagccgt tcaatggctc
     5821 ccacccacaa ttccagatcg ggattgacgg ggttttttac cagtacggga atatccaatc
     5881 cctgtacggc atcggagatt tcctgcatca aaaagggatt gacggtactt ctcgccccta
     5941 tccagagcat atctactcca tatttgactg cttcgtacac atgctgggtc gaggccactt
     6001 cggtgcaagt gtacataccg gtttgttctt tgacgagttt gagccaggcc aagcctttgg
     6061 tgccgattcc ttcgaaggca ccgggccggg tacggggctt ccaaataccc gctctgaaaa
     6121 ttcgtatacc tttttcggcc aactgcctgg ccgttgttaa tacctgttcc tctgtttcga
     6181 cactgcaagg accggaaata acgatgggcc ttgtcagatc gaggtcggga aatagctgtc
     6241 tattgaattg catatagttt aagtataaaa tgtataatta ttaactcgtc aggaccggtt
     6301 ttccggctga actgcacttt tgtttatgta agtaattgca gaccctccta cgggcttcac
     6361 gaatttgttc ttcgggactg cagagcgaaa ttcgtatata acggttgccc ttgctgccaa
     6421 aaataaaacc gggagccaca aatacccttg ccttgtataa aagttcatcg cacaattccg
     6481 cagcgtctgt ataatcatcg ggaattcgcc cccacaaaaa gatgccttcc tggccggcgt
     6541 cgaaggtgca atgcaaactg cacatgagtt cctctataag aacccggcgc gaagaatacc
     6601 gcctgttgag ttctttgtac cagctccgct ccgattgcag agctttgacg gctgccaact
     6661 gcatgggcag aaattgaccg gaatccacat tgctttttaa cctgacgacc caggaaacaa
     6721 attccggatt accggccagc atgcctatac gccaaccggg catattgtgc gatttgctca
     6781 gagaattcag ttccaggcaa cagtctttgg ctccgggaac agataaaata ctaaggggat
     6841 ggttgtttaa aataaaactg taaggattgt cgttgcaaac aacaataccg tgacggcggc
     6901 caaaatcgac cagtttttcg aaggtgcttt tttgtgcagg tgctcctgtg ggcatatgag
     6961 ggtaattgac ccacataagt tttacccggc tcaagtccat tttttccaag gcttcaaaat
     7021 cgggttgcca gccgtttttt tcgtcgagtt cgtattcgag cacttcggcc tgcagcagct
     7081 tacaaacggc tctataagtc gggtaacccg gattgggcag caacacggcg tctccgggat
     7141 tcaggaaagt catggaaaca tgcatcactc cctctttgga acctatcagg ggaagaattt
     7201 ccctgtcggg atccaaatcc acgccgtacc attccttgta ccaggcggca aaagccgaac
     7261 gcaattcttt acggccaaca ttgggctgat agccgtgagc cgtcttttgc gcggcttctt
     7321 tacacaatgt ttcgacaacc tctgcccggg gcattaaatc gggactgcca atggccagac
     7381 tgataatatc tttgccttga gcgttgagca tggcaacttc acggagtttg gacgaaaaat
     7441 agtattcgtt tattccctca aggcgtttgg caggttttat ttctttctgc atggttatgg
     7501 attttattaa ataaaaaaat cccgcccaat taggcgggat cgctatcaat atatctcttt
     7561 atctgctcag atttttttac aatattatcc cgcccttaac ttgtccagct aaagtaaaaa
     7621 taataagagt aaacaaaaag ctgagtagcc atatgtcttg aataattcgg ggcaaatata
     7681 gtcttttttt ttattcccaa ccaaaaaaac atttttttgt gaaataatca gtcggtttta
     7741 cgaaatatcc ggtgttctcg ctgaacaata tcgcctctgt cgcagaggaa cctgaacact
     7801 ttgagagatt tattgctgtc gtaaggcaag ctgtctatca actcttccaa gtcaggatta
     7861 ctgttctcta aagccgcgag catatcccgg ctgatgtctt caaattcata ttgacgcaaa
     7921 ccttcctgct gtttcctaag gcaaacatcg cattgccggc aattggatac ccgcttttga
     7981 ccgaaatatt gcagcagcct tttacttcga cagggttgat cttcggcggc ataagcctgc
     8041 atggcctgaa ttctttccac ataacgcttt ttgcgttctt cgtacacttc gggaccaatg
     8101 tgcaggcgct cgctttcttc tctggactga gtataacaaa taaaaggcgt tttttttcgg
     8161 ggtatgtaat gaattactcc ctgacgggat aaatacaaca agctttcgta cacttcttcc
     8221 ccggtcattt gcagcctgga agccagtact tcttcctgta ttcgcacaaa atcggaaaac
     8281 aaaccggtat aagaacgcag caatacctgc aatacgcgat ctgttttgtt tccggagcgt
     8341 ggaagatgat acaaatcttc tctgctgctc aaaaaataca aacgggaagt gctgtcttgt
     8401 tcatcggtca attccagata gccggccagc tccagtattt tcaagccgtt gtatgcctgc
     8461 agcatgttca ggtgaaattt tccacaaaaa tcctgtaaat caaagggaaa attcattccc
     8521 tgacctgaac ccacggccaa ctgataataa ttgcccagcg cttcgtagac tttgcggatg
     8581 tatttcttat tgggaaagtt gtcgctgagt ctttttttaa gcagagtgct gtctgatttg
     8641 tggtagagca acaccgcata ggatttttgt ccgtctcgac ctgcccggcc ggcttcctga
     8701 aaataggctt cgagcgagtc gggcaaatcc atgtgaatca ccgtgcgcac atcgggctta
     8761 tctatgccca tgccaaaggc attggtggcc acaatcaccc ggcaagttcc gtttttccag
     8821 gcggcttggc gttcttgttt ggtctgatca ttgagtccgg catgaaaatg agccgcctct
     8881 acgccctctc cttttagaaa atttgcaatt tgtttggttt tggccctgtt gcgcacgtaa
     8941 acaatagagg ttccgtctac accttttaac acctgcaaaa gagctttgtc tttgtcctgg
     9001 gtctctctga ccacataagc caggttttta cgctcaaaac tgactttaaa aacattaggg
     9061 gcagcgaaat gcagtttatc ttgtatgtct tccacaacct caggtgtagc agtggccgtg
     9121 agagcaagga cgggtatgga gggaaaatac tcgcgtacgc gggcaatatt caggtaagat
     9181 ggtctgaagt cgtaacccca ttgcgaaata caatgcgctt catccactac cagcagattt
     9241 accggcaggc ccagggctct ttgcaaaaag agatcgctgc ccagtctttc gggagatacg
     9301 tacaagaatt taaaatctcc gtacaggcag ttgtccaaca ctatttgtat ctcctgccgg
     9361 gtcattccgg agtgcaaaca ggcggcgcga atgtccatag acttgagcct ctccacctga
     9421 tcgcgcatca gggcaatcaa tggagtgata accaaacaaa tgccttccat tgcaagggca
     9481 ggcacctgaa aagtaagaga cttgccgcct ccggtgggca tcagacccag ggtgtcctta
     9541 ccggcggcaa cggactcaat aatttcgagc tgtaattccc tgaaatcctc gtagccccag
     9601 tactgtttca gaatgtcgtg ataaatggtc attatccgga gaatttagct gcgccggtgc
     9661 ctgaagctcc caacagttcg aaaggcaaat gaacaaaaac ggaatcttaa tcttcaacaa
     9721 ctttgatatc cctgacaagc aactgaatgg aagaattccc gttgtaatag ttttcttcga
     9781 tgttataaca aatatcaaaa ggattgaacg acttgacatg atcgttgtga gcgtgcatac
     9841 cgaaggctat cccattcatt atgttttcgg aatgactgtc gaccagttca agcttgagat
     9901 gttcctggtc gcggcctacc aagcggctgg taccgtaatc aaaaaccctg cgggtagaaa
     9961 agaccggctt attgttgtcg ggtccaaagg ggttgaattg cttgagcacc ctgaaaaatt
    10021 taggcgtaat ttcctggaac tttatttcgg catcaatatc gatggtaggg gtaagttgtt
    10081 ccaacgatat ggtatcggcc acatattgct caaaacggcg ggtgaactcc tccaccttct
    10141 ccattttcat ggataagccc gccgcatagg tatgcccgcc aaaattttcc agcaaatcac
    10201 gacaggaatc gatggctttg taaatatcaa aacccggtac ggaacgagcc gaaccggtgg
    10261 ccaaatcgtc ggaacgagtc aaaaccacgg ccggacggta gtattcctcc gacaacctgg
    10321 aggcaacaat gccaatcact cctttgtgcc attcctcgtt atagatcact attcctttac
    10381 ggtcgtccag attggtacag gccttaaggg ccgcattggc ttcttcggta atgttcctgt
    10441 ccaagtcttt gcgggtttga ttatactggt caatttgcaa gcttttctcc ttggcaaaac
    10501 gcagattttt ggtaaccaac agttcgacgg cttccccggc cgactgaatt cgaccggaag
    10561 cattgagcct ggggccgatc ttaaagacaa tatcgctgat ggtgatttct ttacctacca
    10621 atccggaaag ttgaataata gcttttaagc ccacactagg tttggtattc aactgtttga
    10681 gaccgtgaaa ggccagaata cgattttcgc ccgtaatagg aacaatatcc gaggctatac
    10741 ttactaccac caaatcgagt aaaggcaaca agcgatcgaa agggataccg ttattttgag
    10801 caaaagcttg catgaactta aaaccgacac cgcaacccga taattcctta taggggtatt
    10861 ggcagtcggg acgtttggca tccagtacgg ctacggcatc gggcaagacg tcgtcgggtt
    10921 tgtgatggtc acagatgatg atatctattc ctttatcttt ggcgtaagcc actttttcca
    10981 cggctttaat gccgcaatcc aacacaataa tcagttttac ttggtgctca aaggcataat
    11041 cgattccctg ataggaaata ccgtaaccct ctacataacg atcgggcagg taaaattcga
    11101 tattcaaact gtattggcgt aaaaacttat aaaccaaggc cactgcggta gccccgtcta
    11161 catcgtaatc cccatagaca agaatttttt ctttgttagc gatagccaaa ttcaacctgg
    11221 caatagcttt gtccatatcc ttcatcagga aggggtcgtg caaatcgctc agttgaggtc
    11281 taaaaaattt ttttgcttca ctaaaactgc ttattcctct cagtattagc aactttgcta
    11341 atatcgggct aataccgagt tcttcagcta attgttcttt ctgtgtctct tgttcggggg
    11401 ttaaggttaa taaattccat ttgctcatta gataatatat tattttttct tttgttctcg
    11461 cagaaatttt taaaactgaa cagagaacac tccccgcagt ttcaaaaaaa gttttacaaa
    11521 aatacgtttt tattccgtca ttcagattgt attccatgtg aattttaatc aataaacaag
    11581 aattagatac tatctttgtg gtgcataacg ttatacacta aaacaacgca aaaatcatga
    11641 gtttgcagga ggaaattaac aaagccggag aagtgctgaa acagggaggt gttatactct
    11701 atcctaccga tacggtttgg ggcctgggtt gcgatgccgg caatccgcag gcaatcgaaa
    11761 aaatccacgc catcaaaaaa agagccgaaa ataaagccat gattgtgctt ctggacaatc
    11821 cctataaact caatttatac gttgaagaag ttcccgagct ggcatggaac ctgatagaat
    11881 tgtccgacaa accgctcacc attatttacc ggggagccaa aaacctggct gcaaacctga
    11941 tcgccgaaga cggcagcgta gccatcagaa tcacccggga acaattttca cagagtttat
    12001 gccagtcttt tcgcaaagcc atcgtatcta cttcggccaa tatctcggga acacctgctc
    12061 ccggttcttt cagggatatc tccgcagaaa tcatctcggc cgtcgattat gtggtcgatt
    12121 acagacgaaa tgaacccgca ggcaaagcca agccttcgtc tattataagt ttgggcaaca
    12181 aaggagaaat aaaaataata agaccctaaa aatagggcat aaaacaccag aatggaaaaa
    12241 gataaaaaat acatattgcg ctatgctttg ttggactata tagccactct gatagcctgg
    12301 caactgttta atatttaccg tttttacgaa gttcgttcca ctgtttactt caacaccctg
    12361 ggggattttt taataaatac caaggctatt ttgatgggtg taggaatgcc tttgttctgg
    12421 ctgattgtct acttttattc cggttattac gccaagccgc gacagaaaaa ccacacctcc
    12481 gatttattga gcaccctttt cagtaccctg gtcggatcgc tgattatctt ttttgccgtc
    12541 atcatcaacg actaccccga acaatacaca ctctattaca gagtgtttgc cggcttgttt
    12601 cttatacatt ttttttgcac ctggtttgtc cgttttttac aaacgtccag gctgcttaat
    12661 ttacacgccg aggggaaagt gggtattaat gtcctgatta taggcagcgg aaaagtggcc
    12721 ggcaaactga gccgccagat gcagcaaacc aaaaacagtt ctgcctatct tctgaaaggt
    12781 ttcattcgcg taaacagcga agaaaaggat gtcgcggaac agaatattct gggagataaa
    12841 gagcagctca aagaaatcat caaagaacaa cgtatcgagg ctttaatcct ggccacagac
    12901 cggcaagaca gcgattatac cgagggcctg ctcaaacagc tttatgtgta tcatctggat
    12961 attttgtctg ttatccacaa gcaagacttg cttttttcca acattactct ttattctctg
    13021 ccgggagttc cattgaaata tttgagcccc gtacagttaa aaccttggga acaaaacctt
    13081 aaacgcagta ccgacatact ggtttccgtg ttgggcctgc tggtgctgtc gcccctcttt
    13141 gtcttcctgg ccttgcgcat acgcctggaa tctgcaggcc ccatactcta ccgacaggaa
    13201 cgtttgggaa aagaaatgaa gccgtttacc attttaaagt tcaggaccat gtttgcccat
    13261 tcagaacccg aagggccctt actctcctcg ctcaacgacg agcgtattac tcctttcggc
    13321 cgtttcttgc gcaaataccg cttggatgaa ctgcctcagc tttggaacgt cctcaaagga
    13381 gaaatgtcgc tggtagggcc acgacccgaa aggcgttttt acgtggaacg catagtggat
    13441 caggctcccc aatactacct ggtttacaga gttaagcccg gtattacttc tttgggcatg
    13501 gtaaaatacg gctacgccga ttcggtagaa aagatgattg aacggctgga atacgatctg
    13561 atttacctga aaaatcaatc cctgctaatg gacctgaaga tattgatttt cacttttaaa
    13621 cccatagcgc tgggaaaagg catttgatcg gcgtaaggaa gtaaaagctt gcaagatcc
//
LOCUS       DAOSTD010000047        13463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_4661_length_13463_cov_13.822718, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000047 DAOSTD010000000
VERSION     DAOSTD010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13463
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4661_length_13463_cov_13.822718"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="PLI37_04535"
     CDS             <1..1058
                     /locus_tag="PLI37_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58311.1"
                     /translation="FELITLKEIAKTKSWQNPIDAKVAMVSTKHIEKMECYACHSTWM
                     PQYYGYKYVIDYSKSSVDWLRSPQLYGADGTTADYHKKFAMQPGAPTYGDYSHIRWEN
                     APLGINGEGRVSPLVGVIQTVSTVIDKEGKTVVWNHVAQTEAGYNAIELAPLNPHTTS
                     LKSRECVDCHTNPVAAGYGIDGGIYDAMPGEPRYADVVDAEGNNVSRFTRAQIAAIRE
                     LHGDFMRLLTLDGQQVQTIDTHWPTSTPLTQEQRDFLTRKNSCVACHRDIPKGTIPNR
                     MLTKIAQVTKLSFATSEEHSRIVHSNNLMIAWIKALGVVALIVLAGLIVWGVIERKKV
                     IRFWKRFVAFLKTPLTP"
     gene            complement(1048..2220)
                     /locus_tag="PLI37_04540"
     CDS             complement(1048..2220)
                     /locus_tag="PLI37_04540"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOQ58312.1"
                     /translation="MKRIIDTERIPIKLWLDEIDDQTLYQAKNLANLPFAFQHICLMP
                     DAHSGYGMPIGGVMAADGVIVPNAVGVDIGCGMCAVKTDIKFAPAIRKKMEDILEDIK
                     KRIPVGFSHHKKAQDENLMPQNYDINSLPVVKSEYASALRQLGTLGGGNHFVEIQKEK
                     DNTIWIMIHSGSRNLGKQVADHYNKLAKKLNKEWNSPVNPKVDLAYLPFKTGEAQQYF
                     DEMNYCVDFALANRKLMLTRVQEVFRYYFPNITFGEMINIAHNYAVWEKHFGKKVLVH
                     RKGATSAHKGELGIIPGSQGTNSYIVEGLGNPESFASCSHGAGRKMGRNEAIRSLSLE
                     EEQRKLDRLGIVHAIRRQRDLDEAPSAYKNIVQVMADQEDLVKIVVKLWPLAVVKG"
     gene            complement(2324..2917)
                     /locus_tag="PLI37_04545"
     CDS             complement(2324..2917)
                     /locus_tag="PLI37_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOQ58313.1"
                     /translation="MFLETICIKNGIVQSMEAHRKRMHDTALSFGFNAPELPGLSKLI
                     PAELKKCKKLKCSLVYHEQILNIAFTDYRPKQINSLKLVEANVNYSFKYADRSALDAL
                     LQEKGDCDEILMVKKGYITDTSFSNVVFRKNGEFFTPDTYLLNGSKRQQLLRENKIRE
                     TRIRVNDLYHFDKLYLINAMLDIEDNPGLDVERIRQM"
     gene            complement(2928..3899)
                     /locus_tag="PLI37_04550"
     CDS             complement(2928..3899)
                     /locus_tag="PLI37_04550"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOQ58314.1"
                     /translation="MFISCRQITVRMNEAGKSKTPFLFVVNFEMSEGLFIENPLQQDR
                     VLFDINGVTNAGQVPGVTMEYVFEPYPESVETYKKRFVCAMDALRRGETSLLNLTIKT
                     PIKTSLSLHEIFTYSQAKYRLCVPEKFVCFSPERFVAIRNGKIFSHPMKGTMDAGIPN
                     AAEKLLNDHKETAEHQTVTKLACKDLERVASSVKVNRFRYIDQLKTNKGCILQTSSEI
                     EGTLPSDYMQHLGTLFLKLLPAASITGTPREATLRVIRACEQQPRGFYSGVAGYFDGK
                     NLDSSVLIRFIEKENGKMYFRSGGGITIDSRCESEYREAIQKVYLPF"
     gene            complement(4196..6154)
                     /locus_tag="PLI37_04555"
     CDS             complement(4196..6154)
                     /locus_tag="PLI37_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOQ58315.1"
                     /translation="MNEQALNRHVLSNRIGLLMVLSALFFSCQSLTQPANPNPGPYSA
                     YLFAYFTGNNIEQEAVHYAISADGYNYLALNGNQPVLNSSEISSTGGVRDPHILRGED
                     GKTFYMVLTDMVSAKGWSSNRAMVLLKSTDLINWQSSVVNIQNKYPNQEDLLRVWAPQ
                     TIYDKEAGKYMIYWSMMHKGGPDIIYYAYANEEFTDIEGEPKQLFFPSNGRSCIDGDI
                     IYKDGVYHMFYKTEGHGNGIKHASTTSLTSGQWTESEGYKQQTREAVEGSSVFKHLAD
                     NKYILMYDVYMKGSYQFTESDDLENFKIIDESISMNFHPRHGSIIPITRAELKSLIEK
                     WGKPEGFPELNGNPVLEGYYADPDVLYSEKTGKYYIYPTSDGFDGWSGTYFKTFSSDD
                     LLNWTDEGIILNLETDVSWADRHAWAPCIIEKKMKKGYKYYYYFTAAQKIGVAVADQP
                     TGPFIDSGSALIDFKPEGINRGQEIDPEVFFDPVSGKDYLYWGNGYLAVVELNDDMIS
                     FKENSLKIITPDRTFREGVTVFYREGLYYFLWSENDTRDPDYRVRYGTSKSPLGPIEI
                     PEENLVIAKNPEAGIYGTGHNSVLQIPNKDEWYIVYHRFNWPAGIHMGRAAGFHREVC
                     IDKMEFGTDGSILPVVPTHKSVEPQKEK"
     gene            6336..6872
                     /locus_tag="PLI37_04560"
     CDS             6336..6872
                     /locus_tag="PLI37_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOQ58316.1"
                     /translation="MKEKAKHILVPGAYLVLLLSFLAVGITAADRRSGNRQARSSQEV
                     PVLIEGIYYCDASQSRLYNGPYREYFENGDLKMEMYIVQGKPDGTYVVYYPNAKIKEV
                     RSYLNGVFNGIWRSYSEQGILLAQAEYLNGKKHGTWKIWDEQGTLRYEMYYDRGRKTG
                     NWFMWDETGKLISERKYN"
     gene            6887..7270
                     /locus_tag="PLI37_04565"
     CDS             6887..7270
                     /locus_tag="PLI37_04565"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOQ58317.1"
                     /translation="MKTKVINTINVLWMLLVALTLRAEPTAETKPATKMKSVAELSTE
                     TGTKIFSLSGKVYDLGSSESLAGASIEINGKVCYADLDGNFSIKSDTELNGKYLTVSL
                     ISYESKQIKLEKNQTQELEIALKQR"
     gene            7460..10189
                     /locus_tag="PLI37_04570"
     CDS             7460..10189
                     /locus_tag="PLI37_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ58318.1"
                     /translation="MRRKLKNRLAKGLKTCGFLIVSMFFTFSLRAETGSISGKIFDAE
                     SGEQLIGAAVVIDGTILGAATDLDGEYHIQKVSPGTYTLTISYISYQTVTKENIVVEA
                     GKTTSLNVHLKPAEFLLTEMEVVARANRESENMMLLEQRKALLVTQAVSAHEMSRKGI
                     GDAEVAVTKVSGISKQDGVKNVFVRGLGDRYNSTTLNGFPIPSEDPEYKNIALNFFGT
                     DVIQNISVSKVFNSSLAGDVGGAVIDISSKELVGDEDFSVEFSGGVNTSAIQNPYFVQ
                     DGTDYLGLANRTRPSTDHFNFSNSLDPSQITPVNHSVSISGGKRFEIGDARNPLSFYV
                     VGSHSKSYSAVMDEIVRNTVVDGTVWQDQLGRKYSNSTDQLILGNAQYEFNRHRLAYN
                     LLILHINNQYVGEYTGTNGEKHQDDPDRYLGFLRRQQTNDNTLIVNQLSTEWQLDDRT
                     KFNAGISYNNVTGSEPDRRENYLTKGLGEFYFFTGSDRQKRFYSLLQENDFNLKLNLS
                     YELNDKFNSKKSNFNIGYNGRYVLDNFDALEYVFTAVSGQYQADDLHLDAAYNKQNYT
                     EGRFSMRKGYNNKYRVTKWINSPFAEINYQLASKLTANAGVKVDLVDLNLDYDIEILP
                     AGKVGINNKLYVLPSVNLKYDVNDKNSIRMGLSKTYTLPQSKEISPYQYVNISFTSQG
                     DRNIKPSENYNLDLKWDYYIGTSEIITLNGFYKHILNPIGRIDGASSGGFLTYKNIAE
                     FATVAGAELEIRKDIFNFSSLVKDKRNKLSAGLNASYIYSNLLLQAINTPPRNVQLEG
                     ASPFIINFDLAYNYSYDYVNITNSLVFNYFSSRVHTIGAQGYKNIMESGAPTLDFVSL
                     SRISRHLTIKLKASNLLNSTFLLTRENSDGTHNEILSQFQKGINVSLGLAYDL"
     gene            10246..11415
                     /locus_tag="PLI37_04575"
     CDS             10246..11415
                     /locus_tag="PLI37_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58319.1"
                     /translation="MKKYFMNLMLAVVASTLLFSSCDEKEVKEPIDILIEEGVLRGNL
                     THEVTLDASKTYALAGAFIVEKGGSITIPAGTKIVAEEGFGNYILVAQGGKIFIEGTA
                     EKPVKMVGKTTKPVEGFWGGLIINGYAPLTSSQQEAKTEINNDYSYGGNKTNDNSGSI
                     TYLILESTGARSSADIEHNGLTLNGVGNGTKIENVFVYECADDGIEFFGGSVNVTNLL
                     VVNTDDDMFDFTQGYNGTLKNAYGIWEQGFTSSESDPRGIEADGNFDGNYKDDTNQSN
                     FRVENMTIDLRLDHNNDKTTQMQDVIKVRRGAKATIVNALVKGTGATGDLIDLTDKKG
                     DGDTSSSISLTNSLTNPNAGKEINAKAETPNVKIEAGNTGCPTNIFSWTGYNFSK"
     gene            complement(11541..12446)
                     /locus_tag="PLI37_04580"
     CDS             complement(11541..12446)
                     /locus_tag="PLI37_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HOQ58320.1"
                     /translation="MSSKLSSKNDLPGKSTAFISVILKGLGQIMLQENSITGLLFLIG
                     IFYGSIAMGFAALLATVCGTAVAYLLKYDHAEINQGLYGFSAALVGVAVMLFLKPVFW
                     SWIIVVIGAVLAAMLQHFFIKRKIPAYTLPFVLVTWLILFVCNLFNSGLFTVSAPAIA
                     EVSDSLTDGFKSFGQVIFQSSLLSGILFFLAVFISSPISALYGLAAAIISAILAFYVS
                     VPINDINLGLYGFNAVLCAIVFAGPKVKDGMWVLISLILALVISLLMLKIGITKLTFP
                     FVLATWVTLFLKNKIFLRKSIAYDK"
     gene            12554..13357
                     /locus_tag="PLI37_04585"
     CDS             12554..13357
                     /locus_tag="PLI37_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HOQ58321.1"
                     /translation="MSHKTEKFHLLIVLLTVLFFACTSKQEQVTRLFLIGDSTVADYA
                     NNYDPGKDYYKTRYPVTGWGQVFQELFVKEELKNFEHLFDSDSVLIDNRARGGRSTRS
                     FFEEGRWRSVYEQLKPGDLVLMQFGHNDAAVDKVERYVSVGGYKEYLRMYVLQTRAKK
                     AIPVILTPVCRNYPWKDGRLENVHGEYPQAAKEVAEEMDVFFIDLNQLSMDAFSEIGR
                     DYVSSRYFMNLPAGVYEAYPDGQSDNTHFQPEGARVVAGLVFEALKALI"
BASE COUNT         3839 a         2884 c         3108 g         3632 t
ORIGIN      
        1 aatttgaact gatcacctta aaagagatag ctaagactaa gagctggcaa aatcctatag
       61 atgccaaggt ggcaatggtt tccaccaagc atatagagaa aatggaatgt tacgcctgtc
      121 actctacctg gatgccgcaa tactacggat ataaatacgt gatcgattat tccaaatcct
      181 ctgtcgattg gcttcgctcc ccgcagctct acggagccga tggcacgact gccgattatc
      241 acaaaaaatt tgcaatgcaa cccggtgcac cgacttacgg cgactacagc catatccgtt
      301 gggagaatgc acccctgggc atcaatggcg agggaagggt ttcgcccctg gtcggcgtga
      361 ttcaaacggt gagtaccgtg atcgacaagg aaggaaaaac ggtggtatgg aaccatgtgg
      421 cccaaacgga ggcaggttat aatgccatcg agctggcacc gctcaacccg cacaccactt
      481 cgctgaaatc gagagaatgt gtggattgtc acaccaaccc cgttgcagcg ggatacggta
      541 ttgacggagg aatttacgat gcaatgccgg gtgaaccccg ttatgccgat gtggttgatg
      601 ctgaaggaaa taatgtaagc cggtttaccc gggcgcaaat cgccgcaata agagaactgc
      661 acggcgattt tatgaggctg ctcaccctcg atggacagca ggtgcaaacc atcgacacgc
      721 actggcctac ctccacgccg ctcacccaag agcaacgcga tttcctgact cgcaaaaatt
      781 cttgtgtggc ctgccaccgg gatattccaa aaggcaccat ccccaatcgt atgctgacca
      841 agattgcgca ggttaccaaa ctctcttttg ctacttccga agagcacagc agaatcgtgc
      901 atagcaataa tttgatgatt gcctggatta aggcattggg agttgtggct cttattgtgt
      961 tggccggatt gattgtatgg ggagttattg agcggaaaaa agtgattcgc ttttggaaaa
     1021 gattcgtggc gtttttgaaa acgcctctta ccccttaaca acagcaagcg gccacagttt
     1081 caccactatc ttcaccaaat cttcctggtc ggccatgacc tgaacgatgt ttttatatgc
     1141 ggaaggcgct tcgtccaagt ctctttgccg cctaatggcg tgtacaatcc ctaatctgtc
     1201 aagcttgcgc tgttcctctt cgaggcttaa gcttcgtatg gcctcattac gtcccatctt
     1261 gcgaccggcg ccgtgcgagc aacttgcaaa gctctccgga ttgcccaatc cctctacgat
     1321 ataggagttt gtgccctgcg agccgggtat aattccgagt tcgcctttat gagcggaagt
     1381 ggcgcctttg cggtgtacca gcactttttt cccaaaatgc ttctcccata cggcgtagtt
     1441 gtgagctata ttgatcattt cgccaaaagt gatattcgga aaatagtagc gaaacacctc
     1501 ttgtaccctc gtgagcatca atttgcggtt ggctaatgca aaatcgacgc aatagttcat
     1561 ttcgtcgaaa tattgctgcg cttcaccggt tttgaagggc agataagcaa gatcaacctt
     1621 ggggtttacc ggcgaattcc actccttgtt cagctttttt gcgagtttgt tgtaatgatc
     1681 tgccacctgc ttgcctaaat tccggctgcc cgagtgtatc atgatccaaa tagtattgtc
     1741 cttttctttc tgaatctcaa caaaatggtt gcctcctcca agtgttccga gctgtcttaa
     1801 ggcggaggca tactccgatt ttaccacggg caaactgttg atatcgtagt tttggggcat
     1861 aaggttttcg tcttgcgcct ttttatggtg agaaaaccct acaggaattc tcttttttat
     1921 gtcttctaaa atatcctcca tttttttccg gatggccggc gcaaatttta tgtcggtctt
     1981 gacagcacac ataccgcagc ctatatccac gcctacggca ttgggcacta tcactccatc
     2041 ggcagccatc actcctccga tgggcatccc gtatcccgaa tgcgcatcgg gcatcaggca
     2101 aatatgttgg aaggcaaaag gcagattggc aaggttcttt gcctgataca gcgtttgatc
     2161 gtcaatctca tcaagccaaa gtttaatggg tattctttcc gtatctataa ttcttttcat
     2221 ttcatccagt ttttttcatt tcatccggtt tttctcgttt catccggttt aaatcgtaca
     2281 tcaccagcct ttcctacaaa gatataaaaa ccggtttttc cggctacatc tgccttattc
     2341 gctctacatc caagcccggg ttgtcttcaa tatcgagcat ggcattgatg aggtataatt
     2401 tatcaaaatg gtataaatca ttaactcgta tgcgtgtttc gcggatttta ttctcacgaa
     2461 gaagttgctg ccgcttgctg ccgtttaaaa gataagtgtc gggtgtgaaa aattcgccgt
     2521 tttttctgaa gacgacgttg ctgaaagagg tgtcggtgat ataacctttt ttcaccatca
     2581 atatttcgtc gcaatctcct ttttcttgta gcaaggcgtc aagcgctgat ctatcggcat
     2641 atttaaacga ataattcacg tttgcttcta cgagtttgag tgaattgatt tgtttcggcc
     2701 ggtaatcggt aaatgcaatg ttgaggattt gttcgtgata caccaggctg cattttagct
     2761 ttttgcactt cttgagctct gccggaataa gtttcgataa gccgggcagc tcgggtgcgt
     2821 taaatccgaa gctcagggcg gtgtcgtgca ttcgcttcct gtgtgcctcc atgctttgca
     2881 caataccgtt ttttatacaa atggtttcaa gaaacatgtt gttacgctta aaagggtaga
     2941 tatacttttt gaatggcttc gcggtattcg ctttcacacc ggctgtctat ggtaatgcca
     3001 ccaccgctgc ggaaatacat ttttccgttt tccttttcga taaagcgaat taaaacgctt
     3061 gaatcgagat tttttccgtc aaaataaccg gccacaccgc tgtaaaaacc acggggttgc
     3121 tgttcacaag cgcgtatcac tcttaaagtg gcttcacggg gtgtgcccgt aatagaagct
     3181 gccggaagta gctttaaaaa caaggtcccc agatgttgca tataatcgga gggtagggtg
     3241 ccctcaattt cggagcttgt ctgtaaaata cagcctttgt ttgtttttaa ttgatcgatg
     3301 tatcgaaatc gatttacctt cacagaactt gccacccgct ctaaatcctt gcaggcaagc
     3361 ttcgttaccg tctgatgctc ggcggtttcc ttgtggtcgt tcagtaactt ttcggcggcg
     3421 tttggtatgc cggcgtccat ggtgcctttc atcggatgcg agaagatttt tccgtttctt
     3481 atcgccacaa atcgctcggg cgaaaagcaa acaaactttt cgggaacaca cagacggtat
     3541 tttgcctggc tgtaggtaaa aatttcatgc agcgacagtg atgttttaat aggcgtttta
     3601 atagtgaggt taagtaagga cgtttcccct ctacgcagag catccatcgc acaaacgaat
     3661 cgttttttgt aggtttcaac actttcggga taaggttcaa acacatattc catggttacg
     3721 cccggtacct gtccggcatt ggtaacgccg tttatatcga aaaggacacg gtcttgctgc
     3781 aaggggtttt caataaagag cccttcgctc atctcaaagt ttacaacaaa cagaaacggg
     3841 gtctttgatt tccccgcttc gttcatcctt accgtaattt gacgacaact gataaacatg
     3901 ttgcaaattt acaacatttg tggggcaggg gaaatgcttg ttgagaatat ttgtcaaaca
     3961 cttgcaggta ttaaaaacaa tacctttttc gtacgccaat taaaaaatat tacacaatgc
     4021 cggtaacgca agatacgtat agaaaaagac tgcaacggtt tcaaaactcc ttcaccggaa
     4081 cgcgagcgtg aaatccttgc acatgtccgt cgtataggag agaagcttgc caatgcccac
     4141 ttctaacacc attccggatg ctcctatggg cttcagaatc actatacttg tctgtttatt
     4201 tttctttctg aggctccacg cttttgtgcg tcggaaccac cggcagaatg gagccgtcgg
     4261 taccgaattc cattttgtcg atgcagactt cgcggtgaaa acctgcggca cgtcccatgt
     4321 ggataccggc cggccagttg aagcgatggt aaacgatata ccactcgtct ttgtttggaa
     4381 tttgcagcac cgaattgtgt ccggttccgt aaatgccggc ttcgggattc ttggcgatca
     4441 ctaaattttc ctcagggatt tcgatgggcc ccaaaggcga tttggaggtg ccgtaacgaa
     4501 cccggtaatc cgggtcgcgg gtatcgtttt ccgaccagag aaaataatag agtccttcac
     4561 ggtaaaacac tgtgacacct tcgcggaagg tccggtcggg cgtgattatt ttgaggctgt
     4621 tttctttgaa ggagatcatg tcatcgttga gttcaaccac agccagatag ccattgcccc
     4681 aatacaaata atcttttccg gataccggat caaaaaagac ttccggatcg atttcctggc
     4741 ccctgttgat gccttcgggt ttaaagtcga tcagggcact gcccgaatct ataaaggggc
     4801 cggtgggttg atcggccacg gctactccga ttttttgggc agcggtgaaa taataatagt
     4861 atttatagcc ctttttcatc tttttctcga tgatgcaggg agcccaggca tggcggtcgg
     4921 cccagcttac atcggtttcc aggttgagaa taatgccctc gtcggtccag ttgagcaaat
     4981 cgtcggacga aaaggtttta aagtaagtgc cgctccagcc gtcgaagccg tcgctggtgg
     5041 ggtagatgta gtacttgccc gttttttcgg aataaagcac atcgggatcg gcataatagc
     5101 cctctaatac tgggttgccg ttgagttcgg ggaatccttc aggcttaccc catttttcga
     5161 tcagggattt cagttctgcc cgggtaatgg gaataatgga gccgtgacgg ggatggaaat
     5221 tcatcgaaat ggattcatct atgattttga aattttccag atcatcgctt tcggtgaatt
     5281 gatagctgcc cttcatatat acgtcgtaca tcagtatgta tttattgtcg gccagatgtt
     5341 tgaataccga agagccttct acggcctcgc gggtttgttg tttgtagccc tccgattccg
     5401 tccattgtcc cgaagtcagc gaggtggtgc tggcgtgctt gatgccgtta ccgtggcctt
     5461 cggttttgta aaacatatgg taaaccccgt ctttgtagat gatgtcgccg tctatgcagg
     5521 aacggccgtt ggaggggaaa aacaattgtt tgggttcgcc ttcaatgtcg gtgaattctt
     5581 cgttggcata ggcatagtag atgatgtcgg ggccgccttt gtgcatcatc gaccaataga
     5641 tcatgtattt gcccgcttcc ttgtcgtaaa tggtttgcgg agcccataca cgcaacaggt
     5701 cttcctggtt ggggtattta ttctgaatgt ttaccacgct cgattgccag ttgatcaggt
     5761 cggtcgattt gagcaagacc atcgcccggt tggagctcca acccttggcc gaaaccatgt
     5821 cggtaagtac catataaaaa gttttcccgt cttcgcccct caagatgtgg gggtcgcgca
     5881 ctccgccggt agaactgatt tcgctcgaat tgagaacagg ttggttgccg ttcagtgcca
     5941 gatagttgta accgtctgca ctgatggcgt aatgtacggc ttcctgttca atgttgtttc
     6001 cggtaaaata agcgaacaga taagcgctgt aagggcccgg attgggatta gccggttgag
     6061 tgagtgattg gcaagaaaaa aacagagcgg acaggaccat caataatccg attctgttgc
     6121 tcaggacatg tctgtttaaa gcttgttcgt tcatcgtaaa taatttgttg ttgtttgaaa
     6181 tcaataaaat aaagcgtatc gctgagtaca aaaataagca aaaatgttta gcaaactaaa
     6241 aaaccggttt ttacgatttt ttgtaacaga aacgtagcag gattgatacg gctttttcac
     6301 accacagctt catatttgca gcgtaaaaga acgaaatgaa agagaaagca aaacatattt
     6361 tggtgccggg agcttatctc gttttgctct tatctttttt ggctgtgggt ataactgcag
     6421 cagaccggag gtcggggaat aggcaggcaa ggtcgtcgca ggaagtaccg gtattgattg
     6481 agggtattta ttactgtgat gccagtcaga gcagactcta taacggcccc tacagagagt
     6541 atttcgaaaa tggggactta aaaatggaga tgtacattgt acaaggcaag cccgacggca
     6601 cttatgtggt ctattatccc aacgctaaaa ttaaggaagt ccgttcttat ttgaacggag
     6661 ttttcaacgg aatatggaga tcttacagcg aacagggcat tttgctggct caggccgaat
     6721 acctgaacgg aaagaagcac ggtacgtgga agatctggga cgagcaggga acactgcgat
     6781 atgaaatgta ctacgaccgg ggccggaaaa cgggcaactg gtttatgtgg gatgaaacag
     6841 gaaaacttat atcggaaaga aaatacaact aaacaaataa acggaaatga aaaccaaagt
     6901 aatcaacaca atcaatgttt tatggatgtt gctggtagct ctgacgctta gggcagaacc
     6961 gacggctgaa accaagccgg caaccaaaat gaaatcagtt gccgagctca gcaccgaaac
     7021 cggaacaaaa attttcagtt tgagcggcaa ggtttatgat ttgggttctt ccgagtcttt
     7081 ggccggagcg agcatagaaa tcaacgggaa ggtatgctat gccgatttgg acggaaactt
     7141 tagtattaaa tcggatacag aattgaacgg taaatatctt accgtgagtt taatatctta
     7201 cgagagtaag caaatcaagc tggaaaagaa tcaaacacag gagcttgaaa tcgctttgaa
     7261 acagcgatga gtttagcaat aagcaagaaa aggattggat taggattagc acgaggacct
     7321 ttcgtctttg aggggtcctc tttgtaatat ttttgtaaca caaagatgac aaccttgtca
     7381 ctggaacgcc aacagttcat aacaaggcac ggataccttt gcccaagaaa aattgaaagc
     7441 agacagattg atgatatcga tgcgcagaaa gcttaaaaac agactcgcca agggattgaa
     7501 gacttgtggt tttttgatcg tctctatgtt ttttaccttt tcgctcaggg cagagacagg
     7561 cagtatcagc ggtaaaatat tcgatgcgga atcgggagaa caacttatcg gggccgccgt
     7621 tgttattgat ggcactattt tgggtgcggc tacggatttg gacggcgagt accacatcca
     7681 aaaagttagc cccggaactt ataccttgac tatttcctat atttcttatc aaacggtcac
     7741 aaaggaaaat attgttgtag aagccggcaa aaccacatct ttaaatgttc atctcaaacc
     7801 tgccgaattt ctcttaaccg aaatggaagt ggtggcgaga gctaatcgcg aaagtgaaaa
     7861 catgatgctg ttggagcaac gaaaagcctt gcttgttacc caagcggtga gtgcacacga
     7921 aatgtccaga aaaggaatcg gggacgcaga ggtggccgtt accaaagttt cgggaatatc
     7981 gaaacaggat ggggtgaaga acgttttcgt tcgcggattg ggcgaccgtt ataatagtac
     8041 aacgctcaac ggcttcccca taccttcgga agacccggaa tataagaata ttgctcttaa
     8101 tttttttgga accgatgtca tacagaacat aagtgtaagt aaggtgttta acagctcgct
     8161 tgcaggcgat gtgggcggcg ccgtgattga tattagttcc aaagaactgg tcggggatga
     8221 agattttagc gtggagtttt cgggaggagt caacacttcc gccattcaaa atccgtactt
     8281 cgtgcaggat ggaaccgatt acttaggact tgcaaacaga acgcgtcctt cgaccgatca
     8341 ctttaatttc agcaacagcc tggacccttc tcaaataacg cctgtaaacc actcggtcag
     8401 tatttcaggc ggaaaaagat tcgagatagg agatgccaga aatccactct ctttttatgt
     8461 ggtcggatca cacagcaaaa gctattcggc tgtgatggat gaaatagtaa gaaacacagt
     8521 tgtggacggc acggtgtggc aagatcagtt gggcagaaaa tactccaatt ccaccgatca
     8581 gcttattctc ggaaatgcac agtacgaatt caatcgacat cgactggctt ataacctctt
     8641 aatacttcat atcaacaatc aatatgtggg ggaatacaca ggtacaaacg gagagaaaca
     8701 ccaagacgac cccgaccgct acctgggttt tctaagacgg caacaaacca acgacaatac
     8761 tcttattgtc aatcaattat cgaccgaatg gcagttggac gacagaacaa aattcaatgc
     8821 cggtatttcc tataataatg taaccggctc tgaacccgac aggcgtgaaa attatttgac
     8881 caaaggattg ggagagttct atttttttac aggtagtgat cgccagaaaa gattttattc
     8941 actgctccag gaaaatgatt tcaatttgaa attaaacctg agctatgagc tgaacgataa
     9001 gtttaacagc aaaaaatcca atttcaatat cggatataat gggagatatg tgttagataa
     9061 ttttgacgcg ttggaatatg tgtttaccgc agtcagcggt cagtatcagg cagacgacct
     9121 gcatcttgat gctgcttata ataagcaaaa ctatacggaa gggcggtttt cgatgcgaaa
     9181 aggttataac aacaagtacc gggtgacaaa atggatcaat tcgcccttcg ctgaaataaa
     9241 ctatcaactg gcatccaaac tgacggccaa tgccggagtg aaggttgatt tggttgattt
     9301 gaatttggat tacgacattg agatacttcc tgcaggcaag gttggtataa ataacaagtt
     9361 gtatgtactt ccatccgtga atttgaagta cgatgtgaac gacaaaaatt caatccgcat
     9421 ggggctaagc aagacttata cgcttccgca atccaaagaa atttcaccct atcagtacgt
     9481 aaatataagt tttacaagtc aaggtgaccg gaatatcaaa ccgtccgaaa attataacct
     9541 cgatttaaaa tgggattatt acatcggtac gtctgaaatc atcacattaa acggttttta
     9601 caaacatatt ctcaacccga tcggtcgaat cgacggagcc agttccggtg gttttctgac
     9661 ctacaaaaat attgcggaat tcgctaccgt ggcaggagct gaattggaaa tcagaaaaga
     9721 catctttaat ttttcaagct tagttaaaga caaaaggaat aaactctctg cgggattaaa
     9781 tgcatcttac atttattcaa atctgctgtt gcaagctata aacaccccac ctcgtaacgt
     9841 gcaattggaa ggagcatccc cctttattat caattttgac ttggcttata actacagcta
     9901 cgattatgta aacataacca attctttggt gtttaactat tttagtagcc gggtgcatac
     9961 cattggggca caggggtata aaaacattat ggagagcgga gcaccgacct tagactttgt
    10021 ttcgctttca agaatcagcc gccatctgac catcaaacta aaagcatcca acctgcttaa
    10081 ttcaactttc ctacttaccc gtgaaaatag tgacggcact cacaacgaga ttttaagtca
    10141 attccagaaa gggattaatg taagcctggg actggcatat gatttatgat ttataaacaa
    10201 ccaatagata accaaaacaa ttttaacaat ttaaaaatga acaaaatgaa aaagtatttt
    10261 atgaacttaa tgctggcagt tgtagcaagc actttgttat tcagctcatg tgatgaaaaa
    10321 gaagtgaagg aaccaatcga tatcctgatc gaagaaggag tacttcgcgg aaatttgaca
    10381 cacgaggtaa cactcgatgc cagtaaaacc tacgcactag ccggtgcttt cattgtagaa
    10441 aaaggaggtt ccatcaccat ccccgcaggc acaaagatag ttgccgaaga aggattcggt
    10501 aattatattt tggtggcaca aggaggaaaa atctttatag aaggtacggc agaaaaacct
    10561 gtaaaaatgg ttggaaaaac aacaaaaccg gtagaaggtt tctggggcgg actcattatt
    10621 aacggatatg caccacttac cagctcacag caggaagcaa aaaccgaaat caacaacgat
    10681 tactcgtacg ggggcaacaa gacaaacgat aattccggct cgatcacata cttgatttta
    10741 gaatcgaccg gagcacgttc cagtgcagac atcgagcaca atggtcttac cctgaacggg
    10801 gtaggcaatg gaaccaaaat cgaaaacgtc tttgtgtatg aatgtgccga cgacgggatt
    10861 gaattttttg gaggctctgt aaatgttacc aacttattgg tagtaaatac cgacgacgat
    10921 atgttcgact ttacccaagg ttataacgga acacttaaga atgcctatgg tatctgggaa
    10981 caaggattta caagcagtga atccgaccca cggggtattg aagccgacgg aaatttcgac
    11041 ggaaactaca aagacgacac caatcagtcg aactttaggg ttgaaaatat gaccatcgat
    11101 ttgcgtttgg accacaacaa cgataaaacc acgcaaatgc aggatgttat caaagtacgc
    11161 cgcggcgcaa aagctactat cgttaatgct cttgtaaaag gcaccggagc aacaggcgac
    11221 ttgattgact taacagacaa aaaaggtgat ggcgacacaa gctcaagcat cagcttaacc
    11281 aattccttaa caaatcccaa cgcaggtaag gagattaatg ctaaagctga aactcccaat
    11341 gttaagattg aagcaggtaa tacaggttgc ccaaccaata ttttcagttg gacaggatac
    11401 aatttctcaa aataaaaagt tgattaattc ttaaagagag gctgtcttca cttttgagac
    11461 agcctctttt tttcttcgtt tggttcaagc aataaatgaa gcagtatgtt aagtcttaaa
    11521 ttcaataaac tgaataacca ttatttgtca tatgctatag actttcttaa aaagatctta
    11581 ttcttcagaa ataaggttac ccaagtcgct aaaacaaatg ggaatgtgag ttttgtaatt
    11641 cctattttga gcattagcag actgataaca agagcaagaa tgagcgatat caacacccac
    11701 ataccatcct tgacttttgg tcctgcaaag acaatagcac acaaaacagc attgaagccg
    11761 taaagtccga ggttgatgtc attgatcggt acagagacgt aaaaggctaa aatagcagag
    11821 ataattgctg ctgcaagtcc atatagagcc gaaatgggtg aactgataaa gactgctaaa
    11881 aagaataata tgcccgataa taagctactt tgaaagatca cttgtccaaa gcttttaaaa
    11941 ccgtcggtta gtgagtcact tacttctgca atagccggtg cggatactgt aaataaaccg
    12001 ctgttaaaca ggttacaaac aaatagtatc aaccaggtaa ccaacacaaa gggtagggta
    12061 taggcgggga tttttcgttt aataaaaaag tgctgcaaca tagctgccaa caccgcaccg
    12121 ataacgacga tgatccaaga ccagaatacc ggttttaaga acaacattac tgctactccg
    12181 actaatgcag cactgaagcc ataaagtcct tgattgattt ctgcatggtc atattttagc
    12241 aggtaagcaa cggcagttcc gcatactgtt gctaaaagcg cggcaaaccc catagcaatt
    12301 gatccgtaga aaataccaat cagaaacagt aagccggtaa tgctgttttc ctgaagcatg
    12361 atttgtccga gtcctttaag aattacggag ataaaggccg tagatttgcc cggcagatcg
    12421 tttttagagc tgagttttga actcattttg ttttattttt cggtctgaaa atttttgaag
    12481 ctgctaattt acaaaatgga aataatacaa aacagaaatt ttgtgtaatt tagggacttt
    12541 aaatcccaga tatatgtcac acaaaacaga aaaattccat ttgcttattg tcctgctgac
    12601 cgtcttgttt tttgcctgta cttcgaagca ggaacaggta acccgccttt ttttaatcgg
    12661 cgattccact gtggccgatt acgccaataa ttacgatccg ggcaaagatt attacaaaac
    12721 ccgttatccg gtcaccggct ggggacaggt gtttcaggag ttatttgtta aagaggagct
    12781 caaaaatttt gagcatttat tcgactcaga tagtgtgctg atcgataatc gtgcccgtgg
    12841 aggccgcagt acgcgttctt ttttcgaaga agggcgctgg cgctctgttt atgagcaatt
    12901 gaaacccggc gatctggtgt tgatgcaatt tggtcacaac gatgccgccg tcgacaaagt
    12961 agaacgttac gtaagtgtgg gtggctacaa agaatatttg cggatgtatg tgctgcaaac
    13021 cagagccaag aaagccattc cggtgatatt gactcctgtt tgtcgcaatt atccctggaa
    13081 agatggccgt ctggaaaatg ttcacggcga atatccccaa gccgccaaag aagtggccga
    13141 agaaatggat gtctttttta tcgatctgaa tcaactgtcg atggacgcct tcagcgaaat
    13201 aggccgcgac tacgtgagca gccgttattt tatgaattta cccgccggcg tttacgaagc
    13261 ctatcccgac ggacagtccg acaatacaca tttccaaccc gaaggggcca gggtggtggc
    13321 cggtttggtt ttcgaggcgc tgaaagcgct gatttgattt aagcaagcta atatgaacaa
    13381 gcgaagacta ggaaaaaccg gatttgacat ttcagaaatc agcttaggta cctggcaggt
    13441 gggtggcaga tggggcagcc ctt
//
LOCUS       DAOSTD010000048        13219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_4739_length_13219_cov_13.650334, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000048 DAOSTD010000000
VERSION     DAOSTD010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13219
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4739_length_13219_cov_13.650334"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..716)
                     /locus_tag="PLI37_04590"
     CDS             complement(42..716)
                     /locus_tag="PLI37_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOQ58322.1"
                     /translation="MSIASNIQEINRTLTPESKLIAVSKLQSPESIMQAYEAGQRRFG
                     ESRAQEMTAKHKVLPDDIEWHFIGHLQRNKVKYIIPFVSLIQSVDSPALLEEINKSAE
                     KTSRLVPCLLQVHIAREKSKFGFTPDELKQFMQEGSWKKLGHVRLHGLMGMATFTDNM
                     DQVRQEFRLLRHLFEEIRSGYFSKDESFKELSMGMTGDYQIALQEGSTMVRIGTALFG
                     VREKGE"
     gene            complement(735..2015)
                     /locus_tag="PLI37_04595"
     CDS             complement(735..2015)
                     /locus_tag="PLI37_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOQ58323.1"
                     /translation="MRLYKKTFKVDLFDRIRTNEESLSKMARQLHGYFTYPKLEGPLG
                     AHMKFRGKDILNWNVNDYLGFTQNEEIRRIDEELTRKWGLSYPSGNRIMAGHTSIHEK
                     LESMLADFTHKEDAFVLNYGYQGISSVIDALCDRKDIIVYDVQVHASMIDGVRLHRGE
                     HFSYQHNDMDSLEKALQKAESKLEKDPQGGILLLTQGVFSSTGDYGKLDQIVALKERY
                     SFRIFLNDADGFGVQGKNGSGTAEHFGVSSQIDLYLGSFSKAFASQGCFIAGPELIMN
                     YLRYRTRTQLHSKALPLVFTEGIIERLKYLKKNLSLLKKLHTNADYLRNALLEKGFDL
                     GESNTNIIPIYFPCNIYEALNIVIDLRENYNIFCPVLIYPFILKDQLLLRLMPTVYHS
                     MEDMDFTLNAFVDIRENLKAQKYSTERSDFFKDS"
     gene            2189..3583
                     /locus_tag="PLI37_04600"
     CDS             2189..3583
                     /locus_tag="PLI37_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58324.1"
                     /translation="MKTKITYLFILALAFPWALGAQNVYDDDLYYHPKFESALIKAGN
                     TQDQPSLPANKVTENEIVVAVNNNKSSIDVDVYNRRPSYKNNNPADNYKEKDLVEDSV
                     LISGEEYEDFKYCERIRRFHNDKFTTHITEDGYVNIYIEEGADVDIYYMDDYYWNPWM
                     SPWYFDNFYRPYYYGWGYHGYYGYYSPWRYSGWYYGFYDPWYYSWYSPWYSSWGWDYY
                     YYGYHPAYYYYSPYTVWGYPSYIKTPRKPVNTRTALASRNEVNSRGGIPVSSRQAVGS
                     RGAAGRADSRSSAVYNDFSRAGTTTARATSVRSDNMTRGTGVSRSTAATARSTGTRST
                     VVNSARSSDNNASYRSSTPDNRYSGTGVRQSTTRSSSANSSRSNAVSTTPSSRSGVTT
                     TSPSNSRGSSSSVRSSTSSSSTRSSVSTSSSSSSSYSTPSSSGSSSYSSGRSSSSGSS
                     GSAVRSSSGSSGRR"
     gene            3596..5053
                     /locus_tag="PLI37_04605"
     CDS             3596..5053
                     /locus_tag="PLI37_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58325.1"
                     /translation="MKKTILAVFLFSAFGLMGQEAFDALRYSQSEINGTARSIGMANA
                     FGALGGDLTALGINPAGIAVYRSSEISFSPILSIAGLNSDFKGSVSSDSKVGFGANNF
                     GVVGSYRTYDNSKVANYNFGVTYNRLKNFTRNVSVIGLNRPVSLLDNLSPGSTLENMA
                     WETYLLDDNYNPVLQEGELLDNEQYAIEKGSIGEWNFSMGFNYNYLLYFGASLGVQSV
                     NYEIDTYYKEFFDEGGSMELWNWLKTSGTGINGKIGLIFRPIPELRLGFSYHTPTYYS
                     LRDDYDACLSSYGMPDENNIVEDRSIWISETEPDPVYYQLETPQLFVISAAAQLGQVG
                     LLSVDYERIDYRNIRMRNSSGYPFADVNEDINKFFRLGYKLRAGTEIRLDKQFCFRAG
                     YAFYQSPVANHIEKGNTVIYTPSTAPQYFMEKDAHTASLGFGYRTGVFFFDLAFVEQF
                     RTEHFYPYYNWNGAGEYANIKTNTANIVCSLGVKF"
     gene            complement(5050..7062)
                     /gene="uvrB"
                     /locus_tag="PLI37_04610"
     CDS             complement(5050..7062)
                     /gene="uvrB"
                     /locus_tag="PLI37_04610"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOQ58326.1"
                     /translation="MFKLVSTYQPTGDQPEAIQLLTEGIQQGLSHQTLLGVTGSGKTF
                     TIANVVEQVQRPTLVLSHNKTLAAQLYSEFKSFFPENAVEYFVSYYDYYQPEAYLPHS
                     DLYIEKDLAINDEIEKLRLSATSSLLSGRRDVLVVSSVSCLFGMGNPVDFHNNIQRVH
                     KGQSLSRNVFLRNLVDSLYSRNDVGPGRGNFRVKGDNVDIFLAYSDMVVRVCFWGEEI
                     EAIEQIDSLTGRRLAEFDRFDIYPANLFVTTKERQAQAIRQIELDLAAQVAFFQSIDK
                     AYEARRLQERVSYDIEMIKELGHCSGIENYSRYFDGREPGMRPFCLLDFFPDDFLMVI
                     DESHVTVPQIRAMWGGDHARKQNLVEYGFRLPAALDNRPLRFDEFEAMTSQVIYVSAT
                     PADYELEKSGGLIVEQLIRPTGLLDPVMVVRPSKHQVDDLMEEIRQRAENNERTLVTT
                     LTKRMAEELSAYLKQQGVLCEYIHSDVETLERVNIMDKLRKGQFDVLIGVNLLREGLD
                     LPEVSLVAILDADKEGFLRNHRSLTQTAGRAARHINGKVILYADKITESMRLTLEETQ
                     RRRKKQEEYNKLNNITPTAIQKDNYSVLVSRAAEDSNLKKAYIEPEEINILTDPVVRY
                     MSRPQLEKLIENTRKLMYTASEKMEFLEAAQYRDELYRLEQRLKEL"
     gene            7263..8567
                     /locus_tag="PLI37_04615"
     CDS             7263..8567
                     /locus_tag="PLI37_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOQ58327.1"
                     /translation="MIWNETYECLSRKEMRELQGKRLFNTVERVYYNSPFYRKKMQEL
                     GITPEDIESVEDLAKLPFTVKNDLRDQYPFGLFSSPMSEIVRLHASSGTTGKPIVGGY
                     TRKDLNIWSEVMARTFSAGNCDRGDMFQIAYGYGLFTGGLGAHAGAERIGASVIPISS
                     GNTEKQIQLMTDFGTTALCCTPSYALYLAEMIDKMGVGNKLKLKAGFFGAEPWTEGMR
                     KELENKLSIKAFDIFGLTEIIGPGVAYNCSEQNGLHFNEDHFIPEIIDPNTLQPLPAG
                     EVGELVITTITKEGMPILRYRTKDLTSLNYEKCSCGRTSARMDKIRGRSDDMLIIRGI
                     NVFPSQVESVLLEFSEAAPHYHLFVDRINNTDVFEIHVEVRPEFFSDHLSQMLNLKKK
                     IAHRIQSVLGISAEIKLVEPRSIERSTGKAKRVTDKRNIYNQ"
     gene            8583..9017
                     /locus_tag="PLI37_04620"
     CDS             8583..9017
                     /locus_tag="PLI37_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOQ58328.1"
                     /translation="MLIKQLSIFLENKTGRLTEVTDLLGDAGINLSAFTIAESSDFGI
                     MRVIVSDPDKAKQILAQKGIAVSLTDVICLQCPNTPGALNKTLQLLSAGGISVEYMYA
                     FAVNEFAQVVIRPDKIQECISLLQENHYELIKADDFYAFKEK"
     gene            9096..9896
                     /gene="bamD"
                     /locus_tag="PLI37_04625"
     CDS             9096..9896
                     /gene="bamD"
                     /locus_tag="PLI37_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOQ58329.1"
                     /translation="MKTRFPLILLVILSLASCSEYQKLLKSQDYEAKWQKANEYYEQG
                     KYSKVIELMDELKTIYKGTERAEQALYMLGNSYFQNKDYFSASHEYQIYYKTYPNGTF
                     VEDCRFMSGKAAYLNAPDPRLSQEGTIQAMDELNVFLEYYPLSDRRTEVEIMLNDLME
                     KLAYKALLNCQLYYDLGNYMGNNYESCIVTAILALQDFPSSRYRVDLAFLILKSRYAF
                     AQNSYDYLKEERFRQVIDEYYSFVNEFPQSRYQKEAEQLFETAKKHVK"
     gene            9902..10240
                     /locus_tag="PLI37_04630"
     CDS             9902..10240
                     /locus_tag="PLI37_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOQ58330.1"
                     /translation="MDYKKTNAPTSTQTRDLNALYDEGGNIYESVVVIAKRANQISME
                     LKEELKSKLQEFATYPDNQEEVSENREQIEISRFYEKLPKPTLIATQEFLEDKIYFRN
                     PLKEKSKSKA"
     gene            10237..10698
                     /locus_tag="PLI37_04635"
     CDS             10237..10698
                     /locus_tag="PLI37_04635"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOQ58331.1"
                     /translation="MIQRIQSLYLFLAAVLLTLALFMPWASFFGQEGELFQIKAFKVE
                     QILGKGERSFPFWALGALLTIAAALSFVCLFLFKDRRLQSRFCVFNTILLIGFYLVFL
                     FLVIHVRKSLDASLGVNFGIALPLIALILNLMALRAIIKDETLVQSYHRLR"
     gene            complement(10699..11355)
                     /locus_tag="PLI37_04640"
     CDS             complement(10699..11355)
                     /locus_tag="PLI37_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOQ58332.1"
                     /translation="MKRISLFCSFCILLPALCQSLRAQNKPFSPEFYLGATAGATMSS
                     ITLVPQYVDKTYLFAANGGLSLRYVNEKHFGIQAELNYFQSGWKDDFGYGSTNTYRRE
                     MAFLELPFLMHARIGTKAVNFYLNLGPKFSLFLSEEEYLDSDISYDYHGKALEQPFQW
                     GILGGVGFDVKLKRQNIGLEGRYYYGLSDIFANAVTDDFVTSGLQQISLNLIYYFRLR
                     "
     gene            complement(11356..13080)
                     /locus_tag="PLI37_04645"
     CDS             complement(11356..13080)
                     /locus_tag="PLI37_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOQ58333.1"
                     /translation="MKLRPIIALCTCLVFFTLAAKAQENNIPVVKLFDKEYYKYEVQA
                     SETLYGLSRRFNVSQEEIIAMNPFLIEGLKTGQILLIPVKIFEPDKDELPEKTPDNSA
                     EKSQEKTPGKSCGKSSQKSLKPNEDSLSAVASDSPVLFEKWEYEYYTVEKWRENLSDI
                     ADNFGVDIDEIRLHNPNVPDRLPRGSLLMIPIKKLEVVSEIETAEEEPSEDLEPKIFR
                     KPNIALLLPFMLDDTTANSIERYVEFYEGVLLAAEGLKQHDFSFELSVFDTGNTLESL
                     RKVLTSGWLDRVNYVIAAVNNEQMMFLSDWSVREKKYLILPFSSRIPETEFNPYIYQV
                     NPPHSLNHRALLELDTSYYAGKNILFIRTGNESSDERRELFDTFQISLKHYGIPYIEL
                     TDNEPPAHIAYSFPDTIARHLSLDKENLIVPAPFPMTESNRVITMIGSAANIKSKARI
                     TLLGYPEWLALSKNNLPQLYQLNTHVYSNYYADFQDQKLKQFQKRYAYEFGKDLLNTF
                     PRYALMGYDIMMYFVPKISGSEWETESLQHGFDFVRIGENGGQCNRHLFIIQYTPQRK
                     IISRALPR"
BASE COUNT         3687 a         2842 c         2933 g         3757 t
ORIGIN      
        1 atcgacaaaa ttcccatttg aacccgttgg gatcaacaaa tttattctcc tttttctcgt
       61 accccgaata gggcagtacc gatgcgaacc atggtgcttc cttcttgcag ggcgatttgg
      121 taatcgcctg tcattcccat ggataattct ttgaaacttt catccttgct gaaatatccg
      181 gagcggattt cctcaaataa atgcctgagc aagcggaatt cctgcctgac ctgatccata
      241 ttatcggtaa aagtggccat acccatcagg ccatgcagcc gtacatgacc taactttttc
      301 caactgcctt cttgcatgaa ttgcttaagt tcgtcgggag taaaaccgaa tttacttttt
      361 tcgcgggcaa tgtggacttg caaaagacaa ggaacaagac gagatgtttt ttctgccgat
      421 ttattgattt cctccaataa agccgggctg tctacacttt gaattaatga aacgaatgga
      481 ataatgtatt tgactttgtt tcgctgcaga tgacctataa aatgccattc gatatcgtca
      541 ggtaatactt tatgcttcgc agtcatctcc tgagccctgc tttcgccaaa acgcctttgg
      601 ccggcttcgt aagcctgcat aatgctctct ggggactgta acttggaaac cgcaatcaat
      661 ttagattccg gagtcaatgt tctgttgatt tcctgaatat tcgatgcaat cgacatacta
      721 taaattattt gagcctacga atccttaaaa aagtccgaac gttcagtgga atatttttgg
      781 gctttcagat tttcccgaat gtctacaaaa gcattcagcg taaaatccat gtcttccatg
      841 gaatgataaa ctgtaggcat aagcctaagc agaagttgat ctttcagaat gaagggataa
      901 atgaggacag gacagaaaat gttgtagttt tcccttaaat cgattacaat attcagggct
      961 tcgtaaatat tacaaggaaa atagatgggt ataatattgg tattggattc tcccaaatca
     1021 aaaccttttt ccagcaatgc gttcctcagg taatcggcat tggtgtgcag tttttttaat
     1081 agcgataaat tctttttaag gtatttgagc cgttcgatga tgccttctgt aaaaaccaga
     1141 ggtagggctt tggaatgcaa ttgcgttctg gtgcgatacc tcaaataatt cattattaat
     1201 tccggtcctg caataaaaca tccctgagaa gcaaaagctt tggaaaaaga gcccaggtat
     1261 aagtcaattt gtgagctgac accaaaatgc tcggccgttc cggagccatt tttaccttga
     1321 acaccgaaac catcggcatc gttgagaaaa attctgaaag aataccgttc tttcaaggct
     1381 actatctggt cgagtttgcc gtaatcgccg gtagaactga aaactccctg cgtaaggagc
     1441 aagatgccgc cttggggatc tttttcgagt ttgctttcgg ctttttggag ggctttttcc
     1501 agactgtcca tatcgttgtg ctgatagctg aaatgttccc ccctgtgcag acggacgccg
     1561 tcgatcatgg aagcatgtac ctgaacatca taaacaatga tatccttgcg atcgcacaaa
     1621 gcatcaatga cggaggaaat tccctgataa ccgtaattca ggacaaaagc atcttcctta
     1681 tgcgtaaaat cggcaagcat ggattccagc ttttcgtgaa tactggtatg tccggccata
     1741 atccggttgc ccgaaggata ggagagtccc catttccggg tcagctcttc atctattcta
     1801 cggatctcct cgttttgtgt aaaacccagg taatcattta cattccaatt cagtatatct
     1861 ttgcccctga acttcatatg agccccgaga ggtccttcca gtttaggata ggtaaagtat
     1921 ccgtgcagct gtctggccat ttttgacaaa gactcttcgt tggtacgtat gcgatcgaat
     1981 aaatccacct tgaatgtttt cttgtacagg cgcaaaacta attaaataat tcatacctta
     2041 caaaaatttt caatccggct tttaatcact atcagaggct gagattattg ttaaatctgt
     2101 ttaatttaaa taaacgatct ggcgaaaaaa gtatctttgt attaagcatg tttatcctgt
     2161 tttcaccaaa aaagaaggga gtactgatat gaaaaccaaa atcacttacc tgtttatact
     2221 tgccttagct tttccttggg cacttggtgc acaaaatgtg tacgatgacg acctgtatta
     2281 ccatcctaaa tttgagtctg ctcttattaa ggccggaaat actcaggatc agccttctct
     2341 tccggcaaac aaagtaacgg aaaacgagat tgttgttgct gtaaataata ataaatcatc
     2401 catcgatgtt gacgtgtaca accgcaggcc ctcgtataaa aacaacaatc cggcggataa
     2461 ttacaaagaa aaggacttag tggaagattc tgtgctgatc agtggcgaag aatacgaaga
     2521 ttttaagtac tgcgaaagaa taagacgctt tcacaacgat aaattcacaa cccacattac
     2581 cgaagacggt tatgtgaata tttatattga agaaggcgcc gatgtggata tttattatat
     2641 ggacgactat tactggaatc cctggatgag cccttggtat tttgacaact tttacaggcc
     2701 ctattattac ggttggggtt accatggtta ctatggttat tattcacctt ggcgttacag
     2761 cggatggtat tacggctttt acgatccctg gtattacagc tggtacagtc cctggtacag
     2821 ctcttgggga tgggattact attattacgg ttatcaccct gcttactatt actactcccc
     2881 gtacacagtc tggggatatc catcctatat taagacgcca agaaaaccgg taaatacccg
     2941 tacagcttta gcttcgcgta atgaggttaa ttcaagggga ggaattccgg ttagcagcag
     3001 gcaggcggta ggcagcagag gtgcggcagg cagagcggat agccgttctt cggctgtata
     3061 caacgacttt agccgtgccg gaaccaccac tgcaagagca accagcgttc gtagcgacaa
     3121 tatgaccaga ggcaccggag tttccagaag cacggccgcc actgcacgtt ccaccggtac
     3181 tcgctcgact gtagttaatt ctgccagatc ttccgataat aatgcttcct atagaagctc
     3241 tactcccgac aatcgttatt ctggtaccgg tgtgaggcaa tcaacaacca gaagttcttc
     3301 tgcgaacagc agccggagca atgcagttag tacaactcct tcttccagat cgggtgtcac
     3361 aacgacaagt ccttcaaatt caagaggttc aagcagtagc gtcagaagca gcacgagcag
     3421 cagtagtaca agaagcagcg tatctacatc aagctcatca tcctcttctt attcaactcc
     3481 cagcagcagt ggcagcagtt cttattcttc cggcaggagt tcttcgagcg gcagctccgg
     3541 ttcagccgta cgcagctcat cgggcagttc cggtcgtcgc taaacacttt acgaaatgaa
     3601 aaaaacaatt cttgcagttt ttctgttttc agcattcggc ttaatgggcc aggaagcttt
     3661 tgatgcatta aggtattcac aatctgaaat taacgggaca gctcgttcca tcggtatggc
     3721 caatgctttt ggtgccttgg gaggtgattt gaccgccctg ggtattaatc cggcggggat
     3781 agccgtctat cgaagttcag agatcagttt cagccccatt ttgagtatag ccggtttgaa
     3841 ttcggatttc aaaggttccg tgagcagtga ttccaaagtg ggttttggcg ctaataactt
     3901 tggtgtggta ggctcgtacc ggacttacga taactccaaa gtggccaatt acaacttcgg
     3961 cgttacttac aacaggctga aaaactttac tcgcaatgtg agtgtgatcg gcctgaaccg
     4021 ccccgtttca ttgctggata accttagccc cggcagcacc ctggaaaaca tggcctggga
     4081 aacctatctg ttggacgata attataatcc cgttttgcaa gagggcgaat tattggacaa
     4141 tgagcaatac gcaatcgaaa aagggagtat aggcgaatgg aatttcagca tgggattcaa
     4201 ttacaactat ttactgtatt ttggggcttc cctgggtgta caatcggtca attatgaaat
     4261 agacacttac tataaggagt tctttgacga aggcggcagt atggagttgt ggaattggtt
     4321 aaaaacctca ggaacaggta ttaacggaaa aattggcctg atctttcgcc ccatacccga
     4381 attaaggctg ggcttttctt accacacacc cacttactat tcgctcagag acgattacga
     4441 tgcctgcctg agttcttatg gtatgcccga cgagaacaac atcgtagaag acaggagcat
     4501 ctggatttcc gaaacggagc ccgaccctgt ttattatcaa ttggaaactc cgcaactttt
     4561 cgttatcagt gctgctgctc aactggggca ggttggcctg ctcagcgtag attacgaaag
     4621 gatagattac cggaatattc gtatgcgcaa cagcagtggt tatccatttg cagatgtgaa
     4681 cgaagatatc aataaattct tccggttggg atacaaactg cgtgccggaa cagaaatacg
     4741 cctggacaaa caattctgtt tcagagccgg ctacgctttc tatcaatcac cggtggccaa
     4801 tcacattgaa aaaggaaata cggttattta cacaccttct acggctccac agtattttat
     4861 ggaaaaagat gcacacactg catccctggg ttttggttat cgaacaggag tcttcttttt
     4921 tgacctggct tttgttgagc aattcagaac ggaacatttt tatccctatt acaattggaa
     4981 cggggcaggt gaatatgcca acataaaaac caacacggcc aacattgttt gtagtctggg
     5041 cgttaagttt tagagctctt taagcctttg ctccagccgg taaagttcat ccctatattg
     5101 cgcagcttcc agaaattcca ttttttcgga ggctgtgtac attaatttgc gggtgttttc
     5161 tattaacttt tccaattgag gacgactcat gtagcggacc acaggatctg tcaaaatatt
     5221 aatctcttcc ggttcaatgt aggctttttt caggttagaa tcttcggcag ccctgctgac
     5281 caatacggaa taattgtctt tttggatagc tgtgggtgtg atgttgttta gtttgttgta
     5341 ttcttcctgt ttttttctgc gccgttgggt ttcctccagg gtaagtcgca tactttcggt
     5401 gattttatcg gcgtataaaa tgactttgcc gttgatgtgg cgggcagccc ggccggcagt
     5461 ttgagtcaaa gaacggtgat tgcgcagaaa tccttcttta tctgcatcca gaatagctac
     5521 taaagatact tcgggtaaat cgagcccttc acgcaataaa ttaaccccga tcaatacgtc
     5581 aaattgcccc ttgcgcaact tgtccataat gtttaccctt tccagagttt ctacatcgga
     5641 atgaatgtat tcacaaagta caccctgttg cttgagatac gccgataatt cttcggccat
     5701 acgcttggtg agtgttgtca ccagggttcg ttcgttgttt tccgctctct ggcggatttc
     5761 ttccatcaaa tcatctacct gatgtttact ggggcgtact accatcacgg ggtccaatag
     5821 ccctgtggga cggatcagtt gttctacgat aagtccgccg gatttttcaa gttcataatc
     5881 ggccggggtg gcgctgacgt aaataacctg agaagtcatg gcttcaaatt catcgaaacg
     5941 caaaggacgg ttgtccaaag cggccggaag tctgaaaccg tattcaacca gattttgttt
     6001 acgggcatga tcaccccccc acatcgctct gatttggggt acggttacat gactttcgtc
     6061 aatgaccatt aaaaaatcgt cgggaaagaa atccaggagg cagaagggac gcatgcccgg
     6121 ctcgcggccg tcgaaatacc tggaataatt ctctattccc gagcaatgcc ccagttcttt
     6181 gatcatctct atgtcgtaac taaccctttc ttgtagacgc ctggcttcgt aagctttgtc
     6241 gatagactgg aaaaaagcga cctgggcagc caggtcaagt tctatttgtc ttatggcctg
     6301 agcctgcctt tctttggtgg ttacaaacag atttgcaggg tagatgtcga aacgatcaaa
     6361 ttcagccagt cttctgccag tcagggagtc gatttgctcg atggcttcga tttcttctcc
     6421 ccaaaaacag acccggacca ccatatcgga ataggccaga aaaatgtcta cattatcacc
     6481 ttttacccga aaattaccgc gtccggggcc tacatcgttt ctcgaataaa gactgtcgac
     6541 cagattgcgc aaaaagacgt ttcggctcag cgattggcct ttgtgaacac gctggatgtt
     6601 gttatgaaaa tctacggggt tgcccatgcc gaacaaacaa gataccgagg agactaccaa
     6661 cacatccctg cgaccagata ataaggaaga agtagccgac aacctcagtt tctcgatttc
     6721 atcgttaatg gccaggtcct tttctatata gaggtcgctg tgaggcaaat aagcttcggg
     6781 ttggtaataa tcgtagtagg aaacaaaata ttcaaccgca ttttccggga aaaaactttt
     6841 gaattcactg tatagttgag cggcgagtgt tttgttgtgg cttaatacga gggtagggcg
     6901 ttgcacttgt tccaccacat tggcaatggt aaaagtttta cccgatcccg tcactccaag
     6961 caaagtttgg tgagaaagtc cctgctgaat gccttctgta agtaattgta tggcttcggg
     7021 ctggtctccg gtgggttgat aagtcgaaac aagtttaaac atgagggcag aagataattt
     7081 gcaaaagtag ctttttttaa aagctttgcg aaatctcgtc ctgagggatt tattattttc
     7141 agaatacaaa aaaaaattgt tccttcgcag gattattcgt caaaaggctg attcccggcc
     7201 gatattgaat ataattatat gttttttaca ggattagcct ctttccttta tattttgtta
     7261 atatgatttg gaacgaaact tatgaatgcc tgagtcgcaa agaaatgcgg gaactacagg
     7321 gtaaacgttt attcaacacc gttgagcgcg tttattacaa ttctcctttt tacagaaaaa
     7381 aaatgcagga gctcggcatc actcccgagg atatcgaatc ggtcgaagac ctggcaaaat
     7441 tacccttcac ggtaaaaaac gatttgaggg atcaatatcc ttttggcttg ttttcctcgc
     7501 ccatgtccga gattgtcaga ttgcatgcat cttcgggtac aacaggtaaa cccatagtcg
     7561 gaggatatac gcgtaaagac ctgaatattt ggtccgaagt aatggctaga actttttcgg
     7621 ccggaaattg cgaccggggc gatatgtttc aaattgccta cggttacggt ttgtttacgg
     7681 gagggctggg agctcatgcc ggagctgaac gtattggagc ctcggtgatt cccatttcgt
     7741 cgggtaatac cgagaagcaa attcagctta tgaccgattt tggaaccacc gccttgtgtt
     7801 gcaccccgtc gtatgctcta tacctggcag aaatgatcga taaaatgggg gtaggaaata
     7861 aattgaaact aaaagccgga ttttttggcg cagaaccttg gactgaggga atgcgcaaag
     7921 aactcgaaaa taaattatcc atcaaggctt tcgatatttt tggactgacc gagatcattg
     7981 ggcccggagt ggcttacaat tgcagtgaac aaaacggact gcactttaac gaagatcatt
     8041 ttattcccga aataatcgat cctaatacct tgcagccgct tcccgcgggc gaagtgggag
     8101 agttggtcat tacaaccatc accaaggaag gtatgcccat cttgcgttat cggaccaaag
     8161 acctgaccag tctgaattac gaaaaatgtt cttgcggaag aacttcggcg cgtatggaca
     8221 aaatccgtgg acgcagcgac gatatgttga ttattcgcgg tatcaatgtc tttccctcgc
     8281 aggtggaaag cgttttattg gaattttccg aagcagctcc tcattatcac ctttttgtag
     8341 accggatcaa taataccgat gtcttcgaaa ttcatgtgga agtccgtccc gagtttttca
     8401 gcgaccattt aagtcagatg ttgaatctca aaaaaaagat tgcccaccgc atacaaagtg
     8461 tcttgggcat ctcggccgaa atcaaactgg tcgaaccgcg ctccatagaa cgttccaccg
     8521 gaaaagccaa gagggtgacc gataaacgta atatttataa ccagtaataa gctacaacta
     8581 tgatgttaat taagcaatta tccatctttt tggaaaataa aaccggacgc cttaccgaag
     8641 tgaccgattt actgggcgat gccggtataa acttatcggc tttcaccata gctgaaagct
     8701 ccgatttcgg cattatgcgg gttatagtgt ccgatcctga taaagccaag caaattttgg
     8761 cccaaaaagg tatagccgta tcgctgaccg atgtcatctg cctgcaatgc ccaaatacgc
     8821 cgggagcgtt aaataaaact ctgcaacttc tctctgcggg cggaatttcg gtggaataca
     8881 tgtacgcctt tgccgtcaac gaatttgcac aggtagttat ccggcccgat aaaattcaag
     8941 aatgcataag tctcttgcaa gagaatcact acgaattgat taaagccgat gatttttacg
     9001 catttaaaga aaagtaaaaa acggtcttgt atttttataa ttggagaatc ctgagtatat
     9061 ttgcgggctt tttcggtaga aggtctcaac ttagaatgaa aacaagattc cctttaattc
     9121 tcttggttat actaagcctt gcctcttgca gtgaatatca aaagctcctc aagagtcagg
     9181 attacgaagc caaatggcaa aaggccaacg aatattatga gcaggggaaa tattcaaagg
     9241 tgatagaatt gatggatgaa ctcaaaacca tttacaaggg gacggaaaga gctgaacaag
     9301 ccttgtatat gctgggtaat tcttacttcc aaaacaaaga ttatttttcg gcctcccacg
     9361 aataccagat ttattataaa acttacccca acggcacttt tgtggaggac tgccgtttta
     9421 tgtcaggtaa agctgcttac ctgaacgctc ctgatccccg gctctcgcag gaaggaacca
     9481 tccaagccat ggacgaactg aatgtatttc tggaatacta tcctctaagc gaccgcagga
     9541 ccgaagtgga aattatgtta aacgacctga tggaaaaact tgcctataaa gctttgttaa
     9601 actgtcagtt gtattatgat ctgggtaact atatggggaa taattacgaa tcctgtattg
     9661 tgacagccat cctggctttg caggatttcc caagctctcg ttacagagta gacctggcct
     9721 tcttgatttt gaaatcgcgc tatgcttttg ctcaaaacag ttatgactac cttaaggaag
     9781 aacgttttcg ccaggttatt gatgagtatt acagctttgt aaatgaattt ccgcaaagca
     9841 gatatcaaaa agaggcggaa caattgtttg aaacagcaaa aaaacatgta aagtaaataa
     9901 tatggactac aaaaaaacaa acgcaccaac cagcacacaa acccgtgatt tgaatgcctt
     9961 gtacgacgag ggtggcaata tttacgagag cgttgtcgta attgccaaaa gagccaacca
    10021 gatcagcatg gaattgaagg aagaactcaa aagcaaatta caggaatttg ccacctatcc
    10081 cgataatcag gaagaagtat cggaaaatag ggaacaaatt gaaatctccc gtttttacga
    10141 aaaattgcct aagcctactt tgattgccac tcaagaattt ttggaagata aaatctattt
    10201 ccggaatcct ctcaaagaaa aatcaaaaag caaagcatga ttcagcgtat tcaaagcttg
    10261 tatctcttcc tggcagccgt cttgttgacg cttgctcttt ttatgccttg ggccagtttc
    10321 ttcggacagg aaggggaatt atttcaaatc aaagccttta aggtagaaca aattctgggc
    10381 aaaggagagc ggtcttttcc tttttgggct cttggagctt tgctgacaat agctgccgct
    10441 ctttctttcg tatgcctttt tttattcaaa gacaggcggt tgcagagccg cttctgcgta
    10501 tttaacacca ttctgttgat tggcttttac ttggtatttc tgtttcttgt cattcatgtt
    10561 cgtaaaagcc tggatgcaag cctgggggtc aattttggta tagctttacc attgatagcg
    10621 ttgattttga atttaatggc cttgagggcc atcatcaagg acgaaactct ggtgcaaagc
    10681 tatcatcgtc tcaggtaatc aacgcaagcg gaaatagtat atcagattta aagatatttg
    10741 ctgtaggccg gaagtgacaa aatcgtccgt cacggcattg gcaaaaatat cggataaacc
    10801 gtaataatac cggccttcca ggccaatatt ttgtcgtttt agcttaacgt caaaacctac
    10861 tcctcccaaa attccccatt gaaaaggctg ctctaaagct ttgccgtggt aatcgtagct
    10921 tatatcgctg tcgaggtatt cctcttcgga caaaaagaga ctgaacttcg gacccagatt
    10981 caggtaaaaa ttcactgcct tggtgccaat gcgggcatgc atcaaaaaag gcagttccaa
    11041 aaaagccatt tctctgcggt aagtgttggt tgaaccgtaa ccaaaatcgt ctttccagcc
    11101 gctttggaaa taattcaatt cggcttggat accgaaatgc ttttcattaa cataacgtag
    11161 gctcaggccg ccgttagcag caaaaagata ggttttatcc acgtattgag gaaccagggt
    11221 gatgctcgac atagtggcgc cggccgtagc acccagatag aattcggggc tgaatggctt
    11281 attttgtgct ctaaggcttt gacataaagc cggtagtaga atacagaagc tacaaaaaag
    11341 gctgattcgt ttcatttaac ggggtaaagc tcttgagatt atcttgcgct ggggggtata
    11401 ttgaatgata aacaaatgcc ggttgcattg tccgccattt tctccaatcc gaacaaaatc
    11461 aaacccatgt tgtaaactct cggtttccca ttcggatcct gagattttag gcacaaaata
    11521 catcattata tcgtaaccca tcaaagcata gcggggaaaa gtgttcaaaa gatccttgcc
    11581 aaattcataa gcatagcgct tttggaattg tttaagcttt tgatcttgaa aatcggcgta
    11641 ataattactg taaacatggg tgttcaactg atacaattga ggcaagttgt ttttactcag
    11701 agccagccat tccggatatc ccaacagagt aatacgggcc ttggatttta tattggctgc
    11761 ggagcctatc atcgtaatta cccggttgct ttcggtcatg ggaaaaggag caggaacgat
    11821 caggttttct ttgtctaagc ttaaatggcg ggcaatggtg tcgggaaaac tgtaagctat
    11881 gtgcgcaggc ggttcgttat ctgttaattc tatgtatgga attccgtagt gttttaaact
    11941 gatttggaaa gtgtcgaaca attcgcgacg ttcatcggac gattcattcc cggtgcgaat
    12001 aaacagaatg tttttgccgg catagtagga ggtgtccagt tccaacagag ccctgtggtt
    12061 cagcgaatga ggaggattta cctgataaat gtagggattg aactcagttt caggaattcg
    12121 ggaactgaaa ggtaatatta ggtatttctt ttcacggaca gaccaatcgg acagaaacat
    12181 catttgttcg ttattgactg cggcaataac ataattgacc ctgtccagcc aaccggaagt
    12241 caagaccttc ctgagcgatt ccaacgtatt gcctgtgtcg aaaaccgaca gctcaaagga
    12301 aaaatcgtgt tgtttcagac cttctgccgc caagagcaca ccttcgtaaa attccacata
    12361 gcgttctatg gagtttgcgg ttgtatcgtc gagcatgaag ggtaaaagca gggctatgtt
    12421 gggtttacga aagatctttg gttcgaggtc ctcggaaggt tcctcttctg ctgtctcgat
    12481 ttcggaaaca acctcaagtt ttttgatcgg tatcatgagc aaggagcctc ttggcagcct
    12541 gtcgggtaca ttcggattat gaagtctgat ttcgtcgata tcaacgccga aattgtcggc
    12601 aatatcactc aaattctctc tccatttttc tacggtataa tactcgtatt cccatttttc
    12661 aaacagcacc ggagaatctg aagctaccgc cgatagagaa tcttcgttag gtttgaggga
    12721 tttttgagag ctttttccgc aagatttacc cggtgttttc tcctgggatt tttcagccga
    12781 attatccggg gttttttctg gaagttcatc cttgtccggt tcgaaaattt tcacaggaat
    12841 gagtaggatt tgcccggttt tgagcccttc gatcaaaaaa ggattcatgg ctatgatttc
    12901 ttcctggctc acattaaagc gccggctcag accatacaag gtttcagaag cttgcacctc
    12961 gtatttatag tactctttgt caaacagttt aactaccgga atattatttt cctgagcctt
    13021 tgcagccaga gtaaaaaaaa ccaggcaggt acacaaggca attatgggac gtaacttcat
    13081 cagcgttgtt tttggttgct tatagtgccg gccctccaca gggcagggtc actaatccag
    13141 tgcgcaaatg tagccaaaat ctgctgtttt gcaaatcaga gtataataat accgcacaga
    13201 cagtaaattc aactagttg
//
LOCUS       DAOSTD010000049        13071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_4801_length_13071_cov_17.219806, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000049 DAOSTD010000000
VERSION     DAOSTD010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13071
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4801_length_13071_cov_17.219806"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /gene="porQ"
                     /locus_tag="PLI37_04650"
     CDS             <1..703
                     /gene="porQ"
                     /locus_tag="PLI37_04650"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOQ58334.1"
                     /translation="WSAALRYVDYGRIISAGDNLPEGEFFVKDMAMSGGYAFMLSPCW
                     RAGAGMHLVYSVLEHYTSLGVAVDLGIQYANPEQKIWAGFTLKNLGSQFKPYDESYEA
                     LPWDVQLGFTKGLLHAPFRFTLTAWGFNAWSYPYLEGTTGQVVNNDNFVEKAFKHLLI
                     GLEFVPSDNFQLSLGYNVRRKSELSLAQRTILTGFTAGFSMSVRKLRIGASFAQYHIA
                     GNTLQMTLSTNLGIF"
     gene            707..1408
                     /gene="cmk"
                     /locus_tag="PLI37_04655"
     CDS             707..1408
                     /gene="cmk"
                     /locus_tag="PLI37_04655"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOQ58335.1"
                     /translation="MEKRIIVAIDGHSSSGKSSMARQLAQKQNYLYIDSGAMYRAVAL
                     YCLRKGWIKGTLLDREALVEGLKDITISFSTTDKGLPRICLNGEEVENEIRGMEVSGV
                     VSLVSAVPEVRSYLVDIQRSLAENKGVVMDGRDIGTAVFPEAELKIFLTASADVRAKR
                     RYLELKEKGIEASYEEILHNIEMRDQMDLSRTISPLRKADDAIELDNGRMSIEEQDAW
                     LEARFNEAVYGKNRN"
     gene            1389..2282
                     /locus_tag="PLI37_04660"
     CDS             1389..2282
                     /locus_tag="PLI37_04660"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOQ58336.1"
                     /translation="MARIEIDKHSGYCFGVVKAIAKAEEHLDSKEELYCLGDIVHNNM
                     EVKRLEKKGMKTVHYDEFAGLKNKKILIRAHGEPPSTYKTAERQGLELVDATCPVVLN
                     LQKRVSKAYQEHKNDGTQILIFGKPGHAEVNGLVGQTQNTAIVVEHLADLVKVDLDRP
                     VIMFSQTTKSLEEYRQLVEAITAGINPAVGFEHYDTICRHMSGRVLQVRDFAAANDLV
                     FFVSDAKSSNGKSLYEACKEVNDNTYFISSPGEVRTIDVAGFQRIGISGATSTPRWLM
                     EEVRQEIEKKLQVLGESTQEL"
     assembly_gap    2331..2430
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2431..3158)
                     /gene="meaB"
                     /locus_tag="PLI37_04665"
     CDS             complement(<2431..3158)
                     /gene="meaB"
                     /locus_tag="PLI37_04665"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOQ58337.1"
                     /translation="MKHPENDARYRGLTVNKGIDQPPIINPYSKKRSPRKQYSVEEYV
                     QGILAGNNVILSQAVTLIESLRPDHQETAHEIIDRCLPYAGRSVRWGITGVPGAGKST
                     SIEAFGMELIRQGRKLAVLAIDPSSERSKGSILGDKTRMEALSVEPGAFVRPSPTGGS
                     LGGVARKTRESIVLCEAAGFDTIFVETVGVGQSETAVHSMVDFFLLIQIAGAGDELQG
                     IKRGIMEMSDGIVINKADGNNIDRA"
     gene            complement(3155..>3931)
                     /locus_tag="PLI37_04670"
     CDS             complement(3155..>3931)
                     /locus_tag="PLI37_04670"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOQ58338.1"
                     /translation="SITVYSNATANPVIMLSIRGTVESNNGDVERLFPKTMGSLRLKR
                     TIVPLGDVLIGSIKTETIPVYNQSEAPIELSFNKVPAHMRVMISNSRLEHGNTGIITI
                     NYIPEEARDYGHRQDFFYIVTDPGAKENPANIIIVSANIKEDFSRTGPLDKVPRASFN
                     QTSIDLGKVSGTEKKTAELVLKNLGEKPLIIRKATAACNCIELKADRKEIAPGKESRV
                     RIVFEAGKLIGNLHYTLEFITNDPRQPITKIPLTVNVIAK"
     assembly_gap    3932..4031
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4032..4810)
                     /locus_tag="PLI37_04675"
     CDS             complement(<4032..4810)
                     /locus_tag="PLI37_04675"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOQ58339.1"
                     /translation="MKAYLDLLQRVLDEGVEKKDRTGTGAISIFGHQMRFKLSEGFPV
                     ITTKKLHLKSIIYELLWFLKGDTNVRYLQENGVRIWNEWADEDGNLGHIYGYQWRSWP
                     DYQGGHIDQISKAVDEIKNNPDSRRIIVNAWNVADLPNMNLPPCHMFFQFYVANGRLS
                     LQMYQRSADIFLGVPFNIASYALLLKMVAQVCGLEAGDFVHTLGDAHIYLNHLEQVRL
                     QLSRDPRPLPEMLINPEVKDIFSFKYEDFQLINYDPHPHIKG"
     gene            complement(4810..6786)
                     /locus_tag="PLI37_04680"
     CDS             complement(4810..6786)
                     /locus_tag="PLI37_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOQ58340.1"
                     /translation="MRHFIGYILVLFLSALASCSVSKDIRKADKHYDYGEYNAAAVIY
                     TKAYRRIPAVEKNVRAEVAYRQGECYRFINQSVKAENAYMKAIRYGLPNDSVLLHYAE
                     VLRKNAKYEAAEIQYRQFLENHPGDLQATYGLESCLLAREWKNKAKKYAVNRVQELYL
                     KRGNFSPLLVPAEYKTLLFTSSSIQKEGKKPSKITGLPDNDFFITRLDQNEKWEKPLP
                     IEGAINSEFDEGVGCFDPDGKVLYFTRCVNKSDSTLSGSSAEIYRSVRSGEQWSEPEK
                     LNIFRDTNAVFAHPAISPDGLYLYYVSDMKGGYGGKDIWRSAFYGKSFGPPENLGPQI
                     NTPGDEMFPTIRTDGSLYFSSNTLPGLGGLDIFKAVYNEEDSSWTVENVYDLNSSADD
                     FGITFFGKKEKGLFSSNRNEPRGWDKIWSFGEPEQLTQISGWVTDRFGEPIPEATIRI
                     VNDKGTNTKVVSRRDGSYSYQVEKNAEYVMLGTCRSYLNYSNQFVTTDKDSNYVVNFS
                     LTSLYLPVRIENIFFEFDRWELRPESGPALQELLKLLLDNPHITIEIGAHTDRKGDEE
                     YNLKLSEKRAESVVNYLIKYGIDKERLSAQGYGKSKPAKVDKYMETNYPYLKEGSFLD
                     ESFVETLSLQQQEIADQINRRCEFKVLKTTYKLF"
     gene            complement(6859..8121)
                     /locus_tag="PLI37_04685"
     CDS             complement(6859..8121)
                     /locus_tag="PLI37_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOQ58341.1"
                     /translation="MKQVLQEVQKINIKDYDYGLPDERIARYPAVPRDSSRLLIYDGK
                     NLSQDIFRKIADHLPSPALMVFNNTKVIHARLLFHKKSGAGIEIFCLEPAQPADYIQS
                     FQSLGSCEWYCLIGNSKKWKEGSLNKEIRAGDLTIQLQATRTGQNGRLQGIRFDWQCA
                     QKNLQFSEVLELAGIIPIPPYLKRESENSDEKDYQTVYSKIKGSVAAPTAGLHFSEEE
                     LKNLDRQHILRRELTLHVSASTFQPVKSDRLREHPMHLEFFTVERTLIEDIVRNKNPL
                     IAVGTTSVRTLESLYWFGLQLLENKAAASQDPVVDQWFPYLDHQTYTTEEALTALLDY
                     LDRNKLDRFCASTRLLIAPGYRFRLVDAMLTNFHQPQSTLLLLVAAFVGQDWQRIYRY
                     ALDEGFRFLSYGDSSLLFPGNQQAKTEK"
     gene            complement(8125..10110)
                     /locus_tag="PLI37_04690"
     CDS             complement(8125..10110)
                     /locus_tag="PLI37_04690"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HOQ58342.1"
                     /translation="MSSCIDKKTTGVPGIDLTDLDTTMSPAEDFYQYACGGWVAKNPL
                     PAEYARYGSFDQLAENTQKQLQELVLELDASTQPEGSVARKIAMLYHSAMDSVKLNAE
                     GIEPIRKDLEQIKALENKEALIGHLANMQKRGIWPFFVLYAGADDMNASMNILHTYQS
                     GLGMGERDYYLSPEERNRELREKYVLHVEKMFVLAGFSTTEAQKAASDVMAIENRLAA
                     ASLSREDLRDPYKNYHKMSLEELSKLAPQMDWNLYFESLGDKEIKELNVAQEKFIAEV
                     AVLIHETELNALKNYLSWNLINTAANFLSDDIALQNFDFYGRTLSGSEEMRARWKRAL
                     GAINGTLGEALGQVYVEKYFPPRAKERMLKLVDNLQLSLGERISSLEWMSEETKQKAL
                     EKLGTFHVKIGYPNKWKDYSSLEIKEDSYWENICRASLFSFNEGMDKVGKPVDKEEWL
                     MNPQTVNAYYNPTTNEICFPAGILQPPFFNMDADDAVNYGAIGVVIGHEMTHGFDDQG
                     SQYDKDGNLANWWSPEDSEKFAERTAVLEEYFNKIEVAPGVFANGKFTLGENIADHGG
                     LQVSFHAYTKTDEYKQNQNIDGLTPAQRFFIAYAHVWANNIREQEILRRTAEDPHSLG
                     YWRVNGALPHIDAFVEAFNLKEGDAMFLPKEQRASIW"
     gene            complement(10164..>11531)
                     /locus_tag="PLI37_04695"
     CDS             complement(10164..>11531)
                     /locus_tag="PLI37_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOQ58343.1"
                     /translation="VIVVSHDRAFLDAVCTRTIELSLGRIYDYKLPYSRFVEFRKEMR
                     EQQLRSYENQQKQIQDTEAFIERFRYKATKAIQVQSRIKQLEKMERIEVDEQDNSHLN
                     LRFPPAPRSGSFPVIIEQLSKSYGEHLVVRDVHLSLRRGEKVAFVGRNGEGKSTLVKC
                     ILEEIPYEGSIKLGHKVKIAYFAQNQPQLLDENLSVFETVDKVATGDVRTKIRDILGA
                     FMFGGEASDKKVRVLSGGERSRLAMIRLLLEPANLLILDEPTNHLDMPSKDVLKAAIK
                     AFDGTVILVSHDREFLDGLVEKVYEFSHQKVIEHLGDIRQFLEKKKLDNLRQLEQNPL
                     NAAPGNNKTTVETKAANAFTGNTDRKEKQENSNAPVASNDYAERKALNRKIKKMEKLV
                     QEREDEVSKLEKQLESIQNELNTPQGACRPELHEQYIREKKALDNALSYWEMAATELE
                     SLKIN"
     assembly_gap    11532..11631
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11632..11967)
                     /locus_tag="PLI37_04700"
     CDS             complement(<11632..11967)
                     /locus_tag="PLI37_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ58344.1"
                     /translation="MVSVENIKVEFGDFILLNSISFVINKKDRVALIGKNGAGKSTLL
                     KILAGLLVPGEGRLGIAKETRVAYLPQVMSCPDDCNLIQETEKAFAHIIKLQAEVEHI
                     QHQLSVRKDY"
     gene            complement(12052..>13071)
                     /locus_tag="PLI37_04705"
     CDS             complement(12052..>13071)
                     /locus_tag="PLI37_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOQ58345.1"
                     /translation="GMVEVQGVMKSIWEMPLHEIANELGLMNFIYYLLIKTGFLPPII
                     FMGVGALTDFGPMLRNLRLSIFGAAAQLGIFAVLIGAILIGFTPKEAASLGIIGGADG
                     PTAIFTTIKLAPHLLGPIAIAAYSYMALVPVIIPLVVKLTCTKKELRINMKEQEKLYP
                     SKTEIKNLRAVKIIFPIAVTTVVALFVPGAVPLVGMLMFGNLVKEIGANTFRLFDAAS
                     NSIMNAATIFLGLSVGATMTSEAFLNWTTIGIVVGGFLAFALSIAGGVLFVKVVNLFS
                     KKKINPLIGATGLSAVPMAARVANEIALKYDRQNHVLQYCMASNISGVIGSAVAAGVL
                     ISFLG"
BASE COUNT         3029 a         2930 c         3190 g         3622 t
ORIGIN      
        1 ttggtcggca gctttacgtt atgtggatta cgggcgtata attagtgccg gagacaattt
       61 gcctgagggt gaattttttg tcaaagacat ggccatgagc ggaggctacg cttttatgct
      121 ttctccttgt tggagggcag gcgccggcat gcatctggtt tattcggtgc tggaacatta
      181 tacttctttg ggtgtggctg tagatctggg tattcaatac gccaatccgg aacaaaaaat
      241 ctgggcgggt tttacgctca aaaaccttgg atcgcagttc aagccttacg atgagagcta
      301 cgaagcattg ccctgggatg tccagttggg ttttaccaag ggcttgttgc atgctccttt
      361 ccggtttacc cttactgcct ggggttttaa cgcctggtcc tacccttact tagaggggac
      421 tacgggacag gtagtaaaca acgataattt tgttgaaaag gctttcaaac acttgttgat
      481 aggtttggaa tttgtgccct ccgataattt tcaactcagc ctgggataca acgtccggcg
      541 taaatctgaa ttatcgctgg ctcaacggac tattttgacc ggttttactg caggattttc
      601 catgtctgtg cgaaaactgc gcataggagc ttcttttgct caataccata ttgccggcaa
      661 tactttacaa atgacccttt ctaccaatct gggtatcttt taagcaatgg aaaaacgaat
      721 tattgtggcc atcgatggtc attcttcgag cggtaaaagt tctatggcca gacaattggc
      781 gcagaaacaa aactatctct acatcgacag cggagctatg tacagagctg tggccttgta
      841 ttgcctcaga aagggctgga tcaagggcac gcttttggat cgggaggctt tggttgaagg
      901 actgaaagac atcaccatta gttttagcac tacggacaaa ggccttcccc gtatttgtct
      961 taatggcgaa gaggtcgaaa acgaaatcag gggtatggaa gtttccggag tcgtcagcct
     1021 ggtcagtgct gtgcccgagg ttagatctta tctggttgat attcagcgtt cattggctga
     1081 aaacaagggc gtagtaatgg acgggcgcga cattggaacg gctgtttttc ccgaagcaga
     1141 attgaagatt tttcttacgg catctgctga tgttcgtgcc aaaagacgtt atttggaatt
     1201 gaaagagaag ggtatcgaag ccagttacga ggaaattctc cataacatag aaatgcgcga
     1261 ccaaatggac ctaagccgaa caatcagccc tttgcgcaaa gccgacgatg ccattgaact
     1321 ggataacggc cggatgagta tcgaagaaca ggatgcctgg ttggaggctc gttttaatga
     1381 agcggtgtat ggcaagaatc gaaattgata aacattcggg ttattgtttt ggtgtggtca
     1441 aagccattgc caaggccgag gagcacttgg acagcaaaga agaactttat tgcctgggcg
     1501 atatcgtgca caataatatg gaggtaaaac gcctggaaaa aaaaggcatg aagactgtcc
     1561 attacgatga gtttgccggt ctgaaaaaca aaaaaattct gattcgcgct cacggcgagc
     1621 caccttctac ctataaaacg gctgaacggc agggcttgga attggtcgat gccacctgcc
     1681 cggtagtttt aaacctgcaa aaaagggtgt ctaaagccta tcaggagcac aaaaacgatg
     1741 gaacgcaaat cctgattttc ggcaaacccg gtcatgccga agtaaacggt ttggtgggcc
     1801 aaacccaaaa tacggccata gtggtggaac acttagccga cttggtaaag gtcgatctcg
     1861 acaggcccgt catcatgttt tcgcaaacca ccaaatcgct cgaggagtat cgtcagcttg
     1921 ttgaggcaat aacggccgga ataaaccctg ccgtcggctt tgaacattac gataccattt
     1981 gccggcatat gtccggccgg gtgcttcaag tcagagattt tgcagcggcc aacgatctgg
     2041 ttttttttgt aagcgacgca aaaagctcca acgggaaatc actctatgag gcctgcaagg
     2101 aggtgaatga caacacctat ttcatctcaa gtcccggcga ggttcgtacg attgatgtag
     2161 caggatttca gcgtatagga atcagcggag ccacctctac tccacgctgg ctgatggaag
     2221 aagtccgtca ggaaattgaa aagaagctcc aagttttggg tgaaagtact caggagttgt
     2281 agtaaaaatc gaggagttcg cgggcagcgc tgaagggagt ttttttgtta nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcccggtcga tgttgttgcc gtcggctttg
     2461 ttgatcacaa tgccgtcgga catttccata atgccccgtt tgatgccctg caattcgtcg
     2521 ccggctccgg caatttgaat cagcagaaag aaatcgacca tggaatgtac cgctgtttcc
     2581 gattgtccga ctcccacggt ttctacaaaa atagtatcga agcccgccgc ttcgcaaagt
     2641 acaatacttt ccctggtttt gcgggctaca ccgcccagtg atccccccgt gggcgaaggc
     2701 cttacaaaag ctccgggttc caccgacagg gcttccattc tggttttatc tcccagaatg
     2761 ctccctttgc tgcgttcact gctgggatct atggccagca cggctaattt ccgtccctgc
     2821 cggatcagct ccattccgaa tgcctcaata gaagtgcttt tgcctgcccc gggaactccg
     2881 gtaatacccc agcgtacgga gcgaccggca tagggcagac aacgatcaat tatttcgtga
     2941 gccgtttcct gatgatcggg tctcaaactt tcgataagcg ttacagcctg actcaggatg
     3001 acattgttgc cggccagtat tccctgtacg tattcttcga cggagtattg ttttctcgga
     3061 ctccttttct ttgaatacgg attgatgata gggggctggt ctatcccttt gtttactgtc
     3121 aatccccggt accgagcgtc gttttcgggg tgtttcattt agcgatgacg ttgacagtga
     3181 gagggatttt agttatcggc tgcctggggt cgttggtaat aaattccagg gtatagtgca
     3241 ggttcccgat caatttcccc gcttcgaaaa caatgcggac gcggctttcc ttgccgggag
     3301 caatctcttt ccggtcggct tttaactcta tgcaattgca agcggcagta gcttttctga
     3361 ttatgagcgg cttttcgccc aggtttttta aaaccagttc agcggtcttt ttttccgtgc
     3421 ccgacacctt tcccaaatct atggacgttt ggttgaaaga tgctctgggc actttgtcca
     3481 aggggcctgt tcttgaaaag tcctccttaa tgttggcact gacaattatt atgttggcgg
     3541 gattttcctt tgctccggga tcggtgacta tataaaagaa atcttgccta tgtccgtaat
     3601 ccctggcttc ttcggggatg tagttgatgg taattattcc ggtgtttccg tgttccagcc
     3661 gtgagttgga aatcataacc ctcatatgag cgggcacttt gttgaaactc aactctatgg
     3721 gggcctcgct ttgattatac acaggaatgg tttccgtttt gatgcttcct atcagcacat
     3781 ctcccagagg aacgatggtt cttttgagcc tgaggcttcc catcgttttg ggaaacaaac
     3841 gttctacatc gccgttgttg gattcgaccg tgcctctgat gctgagcatt attaccggat
     3901 tggccgtggc attggaataa acggtgatgc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn ncctttgata tggggatgcg ggtcgtaatt aatcaattgg aaatcttcgt
     4081 atttaaacga gaagatgtct ttgacttcag gattgatcag catttcaggc aaaggcctgg
     4141 ggtcgcggga gagctgaagc ctgacctgct ccaggtggtt taaatagata tgagcgtctc
     4201 ccaaagtatg cacaaaatcg ccggcttcca gtccgcaaac ctgggccacc attttgagga
     4261 gtaaggcata agaggcaata ttgaagggta cccccaaaaa aatgtccgca ctgcgctggt
     4321 acatttgcaa gctgagccgt ccattggcaa cgtaaaactg gaaaaacata tggcagggcg
     4381 gcaggttcat attgggcaag tcggccacat tccaggcatt tacgataatg cgacgcgaat
     4441 cgggattgtt ttttatctcg tcgacggcct tgctgatctg atctatgtgg ccaccctggt
     4501 aatcgggcca ggagcgccac tggtagccgt agatatgtcc cagattaccg tcttcgtcgg
     4561 cccattcatt ccatatacgc actccgtttt cctgtaaata gcggacgttg gtgtcgcctt
     4621 tcaaaaacca gagcaattca tagatgatgg atttaaggtg cagttttttg gtggtgatca
     4681 caggaaagcc ttcgctgagc ttaaaacgca tttgatgtcc aaatatacta atggctccgg
     4741 ttccggtgcg gtcttttttt tctactcctt cgtcgagtac gcgctgcaac aggtctaaat
     4801 aggctttcat cagaataatt tataggttgt tttcaagact ttaaattcac atcgccggtt
     4861 gatctggtcg gctatttctt gttgttgcaa agataacgtt tctacaaaag attcgtccaa
     4921 aaaactgcct tcttttaagt aaggataatt ggtttccata tatttgtcga ctttggcagg
     4981 cttgcttttt ccatagccct gagcgctgag gcgctctttg tctataccgt attttatcag
     5041 ataatttaca acggattccg cccttttttc ggaaagtttc aggttgtatt cctcgtcgcc
     5101 cttgcgatcg gtgtgggctc ctatttcgat ggttatatgc ggattgtcga gcaacagttt
     5161 aaggagttcc tgcagggccg gtcccgattc gggcctgagt tcccagcgat cgaattcaaa
     5221 aaatatattt tcgatgcgca caggcaaata caaagaagtc aggctgaaat taaccacata
     5281 attgctgtcc ttgtctgtgg ttacaaattg attggagtaa ttcagatatg accggcaggt
     5341 gcccagcata acgtattcgg catttttttc tacctggtaa ctgtagctgc cgtctctgcg
     5401 cgagaccacc ttggtattgg tgcctttgtc gtttactatt cggatggtgg cttcgggaat
     5461 gggctctcca aaacggtcgg tgacccagcc gctgatttgg gttaattgtt cgggttcgcc
     5521 gaaagaccag attttgtccc agcctctggg ttcgttacgg ttggaggaga ataagccttt
     5581 ttcctttttg ccgaaaaacg taatgccgaa atcgtcggca ctggagttga ggtcgtatac
     5641 attttctacc gtccagctac tgtcttcctc gttgtaaaca gctttgaaga tatccaatcc
     5701 acccagtccc ggcaaggtat tggacgagaa atacaggctg ccgtctgtcc tgatggttgg
     5761 aaacatctca tcgcccgggg tatttatttg ggggcccaga ttttcgggcg ggccaaaaga
     5821 tttcccgtaa aacgcagaac gccagatgtc tttgcctcca taacctccct tcatgtcgga
     5881 aacataatac aagtacaatc cgtcgggaga aatagccgga tgagcaaaga cggcgttggt
     5941 gtctctgaaa atgtttaatt tttcgggttc actccattgt tcccccgaac gaaccgagcg
     6001 gtaaatctcg gcgctggaac ctgaaagagt cgagtctgat ttattgacac aacgggtaaa
     6061 atagagcact ttcccgtccg gatcaaaaca gcccaccccc tcgtcaaact cggaattgat
     6121 ggccccttct atgggaagag gtttttccca tttttcgttt tggtcgagcc tggtgataaa
     6181 aaaatcgttg tcgggcaggc cggtgatctt actcggtttt ttgccttctt tttgtatgga
     6241 agacgaagta aaaagcaggg ttttatattc tgccgggacc agcaagggcg aaaaattacc
     6301 ccttttcaga taaagctcct gcactctgtt gacagcgtat ttttttgctt tgtttttcca
     6361 ctcacgggcg agcaggcagg attcaagtcc ataggtggcc tgtaagtcgc cgggatgatt
     6421 ttcgagaaat tgccggtatt gaatttcggc ggcttcgtac ttggcatttt ttcgcaaaac
     6481 ttcggcataa tgcaatagga cgctatcgtt cggcagaccg taacgaatgg ctttcatata
     6541 ggcattctcg gctttaacag attgattgat aaaacgataa cattcgcctt gacggtaagc
     6601 gacttctgct cttacatttt tttcgacggc aggaatgcgc cggtaggctt tggtataaat
     6661 gacagcagca gcgttgtatt cgccgtagtc gtagtgttta tccgccttac ggatatcttt
     6721 gctcactgag caggaagcca gggccgacag aaataaaact aaaatgtatc cgatgaagtg
     6781 acgcatacct aattaaacgt aaagccgggt ttcttattgt tatcctcttt ttgcaggtaa
     6841 gaggcttatg gagaaaattt atttttcggt ttttgcctgc tgatttccgg ggaagagcaa
     6901 ggaactgtct ccataactca aaaaacggaa gccctcgtcc agcgcatagc gataaatacg
     6961 ctgccagtcc tgtcccacaa aggcggccac cagtagtagc agggtacttt gaggttggtg
     7021 aaaattggtc agcatggcat ctaccagcct aaagcgatag ccgggggcta tgagcagcct
     7081 tgtggaagca caaaaacgat ccagcttatt gcggtcgagg tagtccagaa gagcagtgag
     7141 agcttcctct gtggtataag tttggtgatc gagatagggg aaccattggt caacaaccgg
     7201 gtcttgcgat gcggccgctt tgttctccaa taattgcagt ccaaaccagt aaaggctttc
     7261 caaagtacgt accgaagtag tgccgacggc aatcaagggg tttttgttcc gaacaatgtc
     7321 ttcgatcagt gtgcgttcca ccgtaaaaaa ttccagatgc atcgggtgtt cacgcaaacg
     7381 atcggacttg acgggttgaa aggtagaggc actcacatgt aaagtgagct cccggcgcag
     7441 aatatgttgc cggtcaagat ttttgagttc ttcttccgaa aaatgcaggc cggccgtggg
     7501 ggcagccacc gaacctttta ttttggaata gacggtttga tagtcttttt catcgctgtt
     7561 ttccgattct cttttcagat aaggaggaat gggaataatg ccggccagct ccaatacctc
     7621 cgaaaactga agattctttt gggcgcattg ccaatcgaag cggatgccct gtagtcgtcc
     7681 gttttggccg gtgcgggtgg cttgaagttg aatagttaag tccccggccc ggatttcttt
     7741 attcaggctg ccctccttcc attttttgga attgcctatc agacaatacc attcgcaact
     7801 gcccaggctc tgaaagcttt ggatgtaatc ggccggttgg gccggttcca gacaaaagat
     7861 ttcgataccg gccccgcttt ttttgtgaaa cagcagacgg gcatggatca cttttgtgtt
     7921 gttaaacacc atcagagccg gagagggcag atggtcggca atcttacgaa aaatatcttg
     7981 tgacaggttt tttccgtcgt aaatcaagag tctcgacgaa tcacggggca ctgccggata
     8041 tcgcgcaatg cgttcgtccg gcaggccgta atcgtagtct tttatgttga tcttttgtac
     8101 ttcttgcaac acttgcttca taaattacca gatggaagca cgttgttctt tgggtaaaaa
     8161 catggcatcg ccctctttca ggttgaaagc ctcaacaaaa gcgtctatat ggggcaaagc
     8221 tccattcacc ctccaatagc ccagggaatg cgggtcttcg gcggttctgc gtaaaatttc
     8281 ctgttccctg atgttgttgg cccaaacatg agcgtaggca ataaagaagc gctgtgccgg
     8341 tgtcaatccg tcgatgtttt gattctgctt gtattcgtct gttttggtgt aggcatggaa
     8401 ggaaacttgc aggccgccat ggtcggcaat gttttcgccc aaagtgaatt tgccgttagc
     8461 aaaaacgccc ggggccactt cgatcttgtt gaaatattct tcgagaacgg ccgtacgctc
     8521 tgcaaacttt tcggaatctt cgggactcca ccaattggcc aggttgccgt ctttatcgta
     8581 ttgactgccc tggtcgtcga aaccgtgggt catttcgtgg cctatcacca caccgatagc
     8641 tccgtaattg acggcatcgt cggcgtccat attgaagaaa ggaggttgca gaatgccggc
     8701 agggaagcag atttcgttgg tggtgggatt gtagtaggcg ttcactgttt gaggattcat
     8761 aagccattcc tctttgtcga cgggtttgcc cactttatcc atgccttcgt taaacgaaaa
     8821 gagcgaagcc ctgcaaatgt tctcccaata agaatcctct ttgatttcga ggctgctgta
     8881 atctttccat ttgttcggat agccgatttt aacatggaag gtgcccagtt tttccagggc
     8941 tttttgtttg gtttcttcgc tcatccattc cagagagctg attctctcac ccagactgag
     9001 ttggagattg tcgaccagtt tgagcatgcg ttctttggcc cggggcggga aatatttttc
     9061 aacgtagacc tgtcccagag cttctcccaa ggttccgttt attgctccca aggctctttt
     9121 ccaacgggcc ctcatttctt cgctgcccga aagggtgcgg ccgtagaagt cgaaattttg
     9181 cagagcgatg tcgtcgctaa ggaaattggc ggccgtattg atcaggttcc agcttaggta
     9241 atttttaagt gcattcaact ctgtctcatg aatgaggact gcaacttcgg cgatgaattt
     9301 ttcctgggct acgttgagct ctttgatttc cttgtcgccc aggctttcga aatatagatt
     9361 ccagtccatt tgaggggcca gtttgctcaa ttcctcgaga ctcattttgt ggtaattttt
     9421 gtagggatcg cgcaaatcct cccggctcag cgaggcagcg gccaggcggt tttcgatggc
     9481 cataacgtcg gaggccgctt tttgggcttc tgtcgtgctg aatccggcca aaacgaacat
     9541 tttttccaca tgcaggacgt atttttcgcg cagttccctg ttacgctcct cagggctcag
     9601 ataataatcg cgttcgccca tacccaggcc gctttggtag gtgtgcagta tgttcatcga
     9661 ggcgttcatg tcgtctgcac cggcgtataa aacaaagaaa ggccagatgc ctcttttttg
     9721 catgttggcc agatgcccga tgagggcctc tttgttttcg agtgccttta tttgttccag
     9781 atccttacgg ataggttcaa ttccttcggc gttcagttta acgctatcca tagcactgtg
     9841 gtagagcatg gctattttgc gggcaacact gccttcgggt tgtgtgcttg cgtcgagttc
     9901 caatacgagt tcctgcaatt gtttttgggt attttcggcc agttggtcga agctcccgta
     9961 acgtgcgtac tcggccggaa gagggttttt ggcgacccaa ccgccgcagg catattggta
    10021 aaaatcttcg gcgggcgaca tggtagtgtc gaggtcggtc aaatcaatac ccggaacacc
    10081 ggtcgttttt ttatctatac agctactcat gctaagtcct gcaataatta gggtgaatac
    10141 tatttttttc attgctcata ttattaattg atttttagag attccagttc ggtggcggcc
    10201 atttcccaat aactaagagc attgtcgagt gccttttttt ctcgtatata ttgttcgtgc
    10261 aattccgggc gacaggctcc ctggggagtg tttagttcat tttggattga ttcgagttgt
    10321 ttttcaagtt tgcttacctc gtcttctcgt tcctgaacga gcttttccat ttttttgatt
    10381 ttgcggttta gagctttgcg ctcagcgtag tcgttgcttg caacgggagc attggagttt
    10441 tcttgttttt ccttgcgatc ggtgttaccc gtaaaagcat tggccgcctt tgtctcaacg
    10501 gttgttttat tgttacctgg agcggcattc aaaggatttt gttccagttg cctcaggtta
    10561 tccagtttct ttttctccag aaactgacgg atgtctccca ggtgttctat tactttttga
    10621 tggctgaatt cgtatacttt ttccactagg ccgtccagaa attcccggtc gtgcgacacc
    10681 agaattaccg ttccgtcaaa agctttgatg gctgctttga gtacgtcttt ggagggcatg
    10741 tcgaggtggt tggtgggttc gtccagaatg agcaggttgg ccggctccaa caagaggcgg
    10801 atcatggcca gccggctgcg ttctcctccc gacagtactc tgactttttt gtccgaagct
    10861 tcgcctccaa acataaaagc tcccagaata tcgcggattt ttgttctgac atcgcctgtt
    10921 gccaccttgt cgacggtttc gaagacgctg agattttcgt ccagaagttg aggctggttt
    10981 tgagcaaaat aggcaatttt gactttatgc cccagcttga tgctgccctc gtagggaatt
    11041 tcttcgagga tgcatttcac caaagtagat ttgccttcgc cgttgcggcc cacaaaggcc
    11101 actttttcac cccggcgcaa gctgaggtgc acgtcgcgga caaccaggtg ttcgccgtag
    11161 gatttactca attgttcgat gattaccgga aagctgccgc tgcggggagc cgggggaaag
    11221 cgcaaattca aatgcgaatt atcctgctcg tccacttcga ttctttccat tttttcgagc
    11281 tgcttaatac gcgattgcac ctggatggct ttggtggctt tgtagcgaaa gcgttcaata
    11341 aaggcttcgg tgtcctgaat ctgtttttgt tggttttcgt agctgcgcaa ttgttgttcc
    11401 cgcatttctt tgcgaaactc aacaaaacgg gaatagggta atttatagtc gtagattctg
    11461 cccagcgaca gttctatggt tctggtgcaa acagcgtcga gaaaagcccg gtcgtgcgat
    11521 accacaatga cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtaatcttt
    11641 ccttacactg agctgatgtt ggatatgttc cacctcggcc tgcagtttta taatgtgagc
    11701 aaaagctttt tcggtttcct gaatgaggtt gcaatcgtct ggacagctca ttacctgagg
    11761 taagtaggcc acccgtgttt ctttggcaat gcctaatctg ccttctcccg gcaccagcag
    11821 gccagctaga atttttagca gcgttgattt acccgctccg ttttttccga tcagggcaac
    11881 gcggtccttt ttgttaatga caaaagagat ggagttcagg aggataaaat cgccaaattc
    11941 aactttaata ttttctacgg aaaccatacc gcaaagttag gtattaaaaa tggagaaaaa
    12001 agagaggcca tactcaaagt acagcctccc ttagatatgt tgactctaat gctatcccaa
    12061 aaaagagatc aataccccgg cagcaacggc tgaaccgatt acaccggaaa tattactagc
    12121 catgcagtat tgcagcacat ggttttgacg atcgtatttc agtgcaattt cgttggctac
    12181 gcgcgcagcc atgggaacgg cgctcaaacc ggtggcacca atgagcggat tgattttttt
    12241 cttgctaaac agattgacta cttttacaaa gaggacaccg ccggcaatag acagtgcaaa
    12301 ggcgaggaaa ccgccgacaa caatgcctat ggtggtccag ttcaaaaaag cttcgctggt
    12361 catggtagca cctacggata atcccaagaa aatggtcgct gcgttcataa tgctgttgga
    12421 agcagcatca aacagacgga aggtgttggc gccgatttcc ttgaccagat taccaaacat
    12481 caacatgccc accagcggga cagcccccgg cacaaacaaa gccactactg tggtaacggc
    12541 tatggggaaa ataattttta ccgctctgag gttttttatc tcggttttgg agggatacaa
    12601 cttctcctgt tctttcatgt tgatacggag ttcctttttg gtacaggtga gtttgactac
    12661 caaaggaata atcaccggaa ccagggccat ataagaataa gcggctatgg cgatgggacc
    12721 cagcaaatgc ggagccagtt tgatggtagt aaatatggct gtaggaccgt cggctccgcc
    12781 aataattccg agagatgcgg cttcttttgg agtaaagcct atcaaaatgg caccaatcaa
    12841 gacggcgaaa atacccagct gagctgcagc cccaaaaatg gacagcctca ggttgcgcag
    12901 catgggcccg aagtcggtca aggcacctac acccataaaa ataatgggtg gaaggaagcc
    12961 ggttttgatg agcaaataat aaataaagtt catcagaccc agttcgtttg caatctcgtg
    13021 caggggcatt tcccagatgc ttttcattac accctgaact tctaccatac c
//
LOCUS       DAOSTD010000050        12690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_4976_length_12690_cov_14.601741, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000050 DAOSTD010000000
VERSION     DAOSTD010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12690
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4976_length_12690_cov_14.601741"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..76)
                     /locus_tag="PLI37_04710"
     tRNA            complement(2..76)
                     /locus_tag="PLI37_04710"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Val,seq:gac)
     gene            complement(150..1613)
                     /locus_tag="PLI37_04715"
     CDS             complement(150..1613)
                     /locus_tag="PLI37_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOQ58346.1"
                     /translation="MGKAQSSPVIAPEISNNNSTESNLSQQGAALLTSSVSDERLGKE
                     SINRLLLEFAIPSIVAMTATSIYNVVDRIFIGNAVGPMAIAGLALVMPIMNLLSAFGS
                     MVGAGASSMVSIRLGQQRREEATSILGNTLILNFLIGGLLTILGLIFLDDLLILFGAS
                     PKTLPYARDFMQIILIANVLNHNFLGLNSIMRASGYPKKAMWSSLLTVGVNVALAPLF
                     IFGFKWGIRGAALATAFAQLSGFIWVTAHFANKKHSLHFKRGYFKLRLKVIGDILSIG
                     LSPFFMHLSASLVVVVINKSLAAYGGEQGDLAIGAYGIVNSVVMLILMLVLGLNMGMQ
                     PVVGYNFGARKNDRMLQAYHNTILIASLVTTSGFFMALLFPRTIVSVFTNDPGMIDLS
                     VTCLKTVVLSFPVVGFQMVTTTFFQAIGKAAWSIFLSLSRQLIFLIPGIIIMPRLLGL
                     KGVWVAMPLGDFLASLLTLIVIITQIRKYRSKPVPAA"
     gene            complement(1663..2835)
                     /locus_tag="PLI37_04720"
     CDS             complement(1663..2835)
                     /locus_tag="PLI37_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOQ58347.1"
                     /translation="MKKISKLLLLLPLLAFFVACEYGIDITPAGKHSAEYADGTGSPG
                     NNQGSGGAQAGVVTAAEWNDLDNWTFWTDLINDTIYNTYPPYWKFYPKHRVAIQINHG
                     ANPVINAALELKKGEYSVWKARTDNLGRAEMWISLFQKTERISMSDYVLYIDGKAWTG
                     ELKSFDQGWNEISLSSAGENSNRVELAFVVDATGSMADELEFLKSDLQDVLDSVSKNN
                     SSLSLFTGAVFYRDEGDEYVVKHSGFTAETATTIDYIGAQRADAGGDYPEAVHTALNT
                     TLTELQWSEQARTRIAFLLLDAPPHYTTAVISSIHASLQLAAAKGIKIIPITASGIDK
                     ETEFLMRLMAMTTNGTYVFITNDSGVGNDHLQASVGEYQVELLNKLMVRLINKYTK"
     gene            2978..4516
                     /locus_tag="PLI37_04725"
     CDS             2978..4516
                     /locus_tag="PLI37_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/carbon-nitrogen hydrolase family
                     protein"
                     /protein_id="HOQ58348.1"
                     /translation="MKEIENLELLFLSIEDYPELKEVMQMAYSSMESSYWKKHHIEKL
                     ISLFPEGQVVLKINGEIAACALSLIVQSGRFEKNHTYKQITGNYSFDTHDPLGDVLYG
                     IDVFVKPEFRGMRLGRRLYDYRKELCEKLNLKGILFGGRIPYYHNYSDKLSAREYIRQ
                     VRERKIHDPVLNFQLSNNFHPIRILKNYLEGDQASEEYAVLLKWDNIYYEEPVSKEIA
                     SPSIVKSSARLGLIQWQMRLYRDIDDLMQQAEYFIDAVSSYRADFALFPEFFNAPLMA
                     AFNHLEEPQAIRKLASFTKEIVKRFAEFSISYNINIITGSMPELVDDKLYNVGYLCKR
                     DGSVERYEKIHVTPDEAKVWGMVGGNQLKTFDTDCGKIGVLICYDSEFPELSRLLADE
                     GMNILFVPFLTDTQNGYSRIRNCSQARAIENECYVAIAGSVGNLPKVNNMDIQYAQSM
                     VFTPCDFAFPVNGIKAEATPNTEMILIVDVDLNLLTELHNNGSVRNLMDRRKDIYELK
                     RVSN"
     gene            4782..>5739
                     /gene="eno"
                     /locus_tag="PLI37_04730"
     CDS             4782..>5739
                     /gene="eno"
                     /locus_tag="PLI37_04730"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOQ58349.1"
                     /translation="MKIKKVEAIEILDSRGNPTVEVDLKLEDGSCGRAMVPSGASTGE
                     REATELRDGDKKRYFGKGVLKAVDNVNNIIAKELEGKCFAGQRAVDYRMLELDGTPFK
                     SKLGANAILAVSMAFARAAAKSSNLPLYQYLGGSNAYLLPVPCMNIINGGQHADNNVD
                     FQEFMIAPHGATSFRESIRMGVEVFHTLKSVLNKKGYSTGVGDEGGFAPNLKSNEEAV
                     DVILEAITKAGFKPGAEVSICLDPAASELWEDGKYKMLKSSGKSLSSDELVALWADWV
                     RQYPIISIEDGLAENDWAGWKKLTDTLGNKIELVGDDLFCTNK"
     assembly_gap    5740..5839
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5840..6220
                     /gene="eno"
                     /locus_tag="PLI37_04735"
     CDS             <5840..6220
                     /gene="eno"
                     /locus_tag="PLI37_04735"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843011.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOQ58350.1"
                     /translation="TLGNKIELVGDDLFCTNKAILKEGIEKGVANSILIKLNQIGTVT
                     ETLETIELADKNSYNNFTSHRSGETVDSFIADLSVAVNAGHIKTGSGCRGERIEKFNQ
                     LIRIEKELGTAGRFAGIAAFKNAK"
     gene            complement(6312..7385)
                     /gene="leuB"
                     /locus_tag="PLI37_04740"
     CDS             complement(6312..7385)
                     /gene="leuB"
                     /locus_tag="PLI37_04740"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOQ58351.1"
                     /translation="MKLNIAVLPGDGIGPEIVEQALNVTKAVCEKFGHELNYKTGLVG
                     AAAIDACGDPYPEETHKLCMDSDAVLFGAVGSLKYDNDPKAKIRPEQGLLSMRKKLGL
                     YANIRPVSVFPSLISRSPLKNRLVRNVDFLCIRELTGGIYFGRPQGRSEDGNSAVDTC
                     TYSREEIERILKLGFEFASKRRKKLTVVDKANVLETSRLWRQIAQEMAPQYPYIEVEY
                     LYVDNAAMRIIQWPRSFDVIVTENMFGDILTDEASVITGSLGMLPSASIGLHTSVYEP
                     IHGSYPQAAGKNIANPFAAVLSAAMMFEQTFGLSEEGKLIREAVTVAMDAGYVTEDIT
                     TGIFRKARSTAAVGRWLVKYIRK"
     gene            complement(7474..8997)
                     /locus_tag="PLI37_04745"
     CDS             complement(7474..8997)
                     /locus_tag="PLI37_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOQ58352.1"
                     /translation="MKTIEILDTTLRDGEQTSGVSFGLQEKLSIARLLLEELKVDRIE
                     VASARVSEGEFKAVRRISEWAGERGLLDRIEILGFIDKGLSLQWASDAGCRVINLLSK
                     GSYRHVTEQLKKTPEQHLKDIKDEVALCRAKGVQVNLYLEDWSNGMMHSPDYVFYLMD
                     SLRDETIGHFMLPDTLGILTPEQTSLYCRQMVERYPGLKFDFHAHNDYDLAVSNVFQA
                     VRAGVTTVHTTVNGLGERAGNAPITSVIAVLHDLLKVQTGIREDRVNRVSRIVESYSG
                     VRIPANKPVIGDNVFTQTAGIHADGDVKSNLYFNDLLPERFGRVRQYALGKTSGKASI
                     KKNLEALGIELDDDAMRLVTERIIQLGDKKELVTQEDLPYIISDVLKQNHQEEPVKVL
                     NYALSLAHGLRPVATVMLDICGEVFQETASGDGQYDAFMKALHKIYSSQKRNLPQLVN
                     YVVTIPPGGRTDAFVQTVIFWSYNNKSFRTSGLDADQTEAAIKATMKMLNIIEEMNM"
     gene            complement(8984..9574)
                     /gene="leuD"
                     /locus_tag="PLI37_04750"
     CDS             complement(8984..9574)
                     /gene="leuD"
                     /locus_tag="PLI37_04750"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOQ58353.1"
                     /translation="MMEKFTTLTSTVVPLPMENVDTDQIIPARFLKATSKEGFGDNLF
                     RDWRYDGNNRPNPDFVLNNPLYGGSILVAGKNFGSGSSREHAAWALAGYGFRVVVSSF
                     FADIFKNNALNSGVLPVVVSEAFLQEIFDTVYADAKSTLTVDLEKQVIRNNANGREES
                     FSINAYKKNCLLQGYDDIDFLLSNKNKIQAWENEND"
     gene            complement(9575..10969)
                     /gene="leuC"
                     /locus_tag="PLI37_04755"
     CDS             complement(9575..10969)
                     /gene="leuC"
                     /locus_tag="PLI37_04755"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOQ58354.1"
                     /translation="MGKTLFDKIWDRHVITTVPDGPTQLYIDRHYCHEVTSPQAFQGL
                     RDRKLRFLRPEKTYAIPDHNIPTLNQDHPIKDPVSRKQVETLQENAREFGIKYFGLGH
                     PKNGVIHVVGPETGLTLPGMTIVCGDSHTSTHGALGAVAFGIGTSEVEMVMASQSILQ
                     TKPKKMRIRVEGKLRPGVSAKDMALYLIAKMTTGGATGYFVEYAGEAVRALSMEGRLT
                     LCNLSIEMGARGGMIAPDQTTFDYIKGREFAPKGAAWQQAVEKWKTLYSDEDAVFDKE
                     EQFKAEDIEPMITYGTNPGMGMAITGRIPLLESIDPAGRASFVKALDYMGFKPGETLL
                     GKKIDYVFLGSCTNGRIEDFRTFTQFVRGRQKAPGVVAWLVPGSKAVERQCREEGLTE
                     ILEQAGFALRQPGCSACLAMNDDKIPAGKYAVSTSNRNFEGRQGPGARTILASPLVAA
                     AAAVTGRITDPRTY"
     gene            complement(10983..12479)
                     /locus_tag="PLI37_04760"
     CDS             complement(10983..12479)
                     /locus_tag="PLI37_04760"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOQ58355.1"
                     /translation="MSDKVFIFDTTLRDGEQVPGCQLNTVEKIQVAKALESLGVDVIE
                     AGFPVSSPGDFNSVVEISKAVTWPTICALTRSVEKDIDVAAEALKYAKRKRIHTGIGT
                     SDYHIKYKFNSNRDEILQRAIACVKYAKKYVEDVEFYAEDAGRTDNEYLARVVEAVIK
                     AGATVVNIPDTTGYCLPDVYGAKIRYLMENVPNIHKAVISTHCHNDLGMATANTMSGI
                     LNGARQVEVAVNGVGERAGNTSLEEIAMILRCHKNLGVHTDINTQHIMHSSRLVSSLM
                     NMPVQPNKAVVGRNAFAHSSGIHQDGVLKNRENYEIMDPKDVGIDENSIVLTARSGRA
                     ALKHRLHLLGVEIGEERLQEVYQEFLKLADRKKVINDEDILLLTGEERSEKKRIKLDH
                     LQVVSGKGVRAVASITLDIAGEKFEATASGNGPVDAGIKALKRIINREMTLQEFLIQA
                     IAKGSDDTGKVHMQVEHQGSVYYGFSGNTDIITASIEAYIDAINKFVD"
BASE COUNT         3280 a         3110 c         2969 g         3231 t
ORIGIN      
        1 gtgattcgta gtggacttga accaccgacc ccttgcctgt caagcaagtg ctctgaacca
       61 actgagctaa cgaatcgttt ttgtgctgca aatgtacggt tttttgctta atcgcaaaag
      121 gactattgca gaaaaagata aagcggcttc tatgctgccg gcaccggctt gctgcggtat
      181 tttctgatct gggtgataat tacgatcagg gtgagcaggg aggccagaaa atcgcccagg
      241 ggcatggcta cccagacgcc tttcagtccg aggaggcggg gcattatgat gatgccggga
      301 attaaaaaga tcaactggcg gctcaggctc aaaaaaatgg accaggcggc tttgcctatg
      361 gcttggaaaa aggtggtggt taccatttga aaaccaacaa cgggaaaact caacacaaca
      421 gttttaaggc aggttacgga cagatcgatc attccgggat cattggtgaa taccgagacg
      481 atggtgcggg gaaacaagag agccatgaaa aaaccgctgg tggtaactaa gctggcaatc
      541 agtatagtat tgtgataagc ctgcaacatt ctgtcgtttt ttcttgcccc aaaattatag
      601 cccaccacgg gttgcatacc catgttaagg cctaatacca gcatcagaat cagcattacc
      661 acgctgttga ctataccgta ggctcctatg gcaaggtcgc cctgttcgcc cccgtaggca
      721 gccagacttt tgttgatgac cactactacc aggctggccg ataaatgcat aaaaaagggc
      781 gaaaggccga tggacaagat gtctcctatc actttcaatc gaagtttgaa atagcctcgc
      841 ttaaagtgca aactgtgttt tttattcgca aagtgggccg tcacccagat aaaaccgctc
      901 aactgcgcaa aagccgtcgc caaggccgcc ccgcgtatgc cccacttaaa cccgaaaata
      961 aacaaaggag ccagggctac attgacaccc acggtgagca aggacgacca catggccttt
     1021 ttggggtaac ccgaagcccg catgatactg ttcagcccca ggaaattgtg gttgagcacg
     1081 ttggctatga gaatgatctg cataaaatcc cgggcgtaag gcaaggtttt tgggctggct
     1141 ccgaacagaa tgagcaggtc gtccaaaaaa atcaaaccca aaatggtgag caaacctcca
     1201 atcagaaaat tcagtatcag tgtattaccc aaaatactgg tggcttcttc gcgacgttgt
     1261 tgcccgagac gtattgacac catcgaagaa gcccctgctc ccaccatcga gccgaaagcc
     1321 gacagtaaat tcattatcgg catgaccagg gccagaccgg caatagccat ggggccgacg
     1381 gcgttgccta tgaatatgcg gtccacaacg ttgtagatcg aagtggcagt catggcaaca
     1441 atgctcggaa tggcaaactc gagcaggagc ctgttgatac tttccttacc cagcctttcg
     1501 tcggaaacag aagaagtcag cagagctgcg ccctgctgac ttaagttact ttctgtcgaa
     1561 ttattgttag atatctccgg ggctattacc ggtgaagatt gcgcctttcc catgaactgt
     1621 caaaaactcg cgcaaaggta gtcatttatt tacaaagccc ctttatttgg tatacttgtt
     1681 tataagccga accatcaatt tattgagcaa ttctacctga tattcgccca cgctggcctg
     1741 aaggtgatcg ttgcccactc cgctgtcgtt ggtgataaac acgtaggtgc cgttggtggt
     1801 catagccatc aggcgcatca gaaattccgt ttctttgtcg atgccgctgg cggtaatggg
     1861 aattatctta ataccctttg cggcagcgag ttgcagcgag gcatgaatac tactgataac
     1921 agcggtagta tagtgaggcg gagcatcgag caacaaaaat gcaatgcggg ttctggcctg
     1981 ttccgaccat tgcagttcgg tcagggtggt attcagtgct gtatgcacag cttcgggata
     2041 atcgcctccg gcatcggccc gctgtgcacc gatgtagtcg atggtcgtag ctgtttccgc
     2101 ggtaaaaccg gaatgtttca ccacatattc gtcgccctcg tcacgataga aaacagcccc
     2161 ggtaaataaa ctcaaactgc tgttgttttt cgatacggaa tcgagcacat cctgcaaatc
     2221 actcttgaga aattccaact cgtcggccat ggaaccggtg gcatccacca caaaagccag
     2281 ttcgacccgg ttggaatttt caccggcact gctcaggctg atttcgttcc aaccctgatc
     2341 aaaactttta agctcgcctg tccaggcttt gccgtcgatg taaagcacat aatcgctcat
     2401 cgaaattctt tcggtttttt gaaacagcga tatccacatt tctgctctgc ccaggttgtc
     2461 ggtgcgggct ttccaaacgc tgtattcccc ctttttcaac tccaaagctg catttatgac
     2521 gggatttgct ccatggttga tctgtatggc gacacgatgc ttggggtaaa acttccagta
     2581 aggcggatag gtgttgtaaa tagtgtcgtt gatcagatcg gtccaaaaag tccagttatc
     2641 cagatcgttc cactcggctg ccgttactac tccggcctga gccccgcccg aaccctgatt
     2701 attacccggg ctgcccgtgc cgtcagcgta ttcggcagaa tgcttccccg caggcgttat
     2761 atcaatccca tattcacaag cgacaaagaa cgccaacaag ggaagtaaaa gcaaaagttt
     2821 ggaaattttt ttcataatga tatgttttta agtattgaaa aaagactacg aacaaataga
     2881 tgaaaaaaga gccgggacgc tacaaaaaag ccatggtctt tttcggattt ctgcgaaata
     2941 aatcttatct ttgtgtttga tttttttaaa acataagatg aaagaaatag aaaacctgga
     3001 acttcttttt ttaagtatag aggattatcc ggaattgaaa gaagttatgc aaatggccta
     3061 ctccagcatg gagagctctt actggaaaaa acaccatatc gaaaagctga tcagcctttt
     3121 tcccgaaggc caggtggtgt taaaaattaa cggcgaaatt gcggcctgcg cactttcgct
     3181 gatcgttcaa agcgggcgtt tcgaaaaaaa ccatacctac aaacaaatta ccggtaatta
     3241 cagcttcgac acccacgatc ccttaggcga tgtgctttac ggcattgatg tatttgtcaa
     3301 acccgagttc agagggatgc gcttgggccg acgtttgtac gactaccgta aggaattatg
     3361 cgaaaagctc aatctcaaag gcattttgtt tggcggccgc attccctatt accacaacta
     3421 ttccgacaaa ttatcggcca gagaatacat ccgccaggtg cgcgaacgca aaattcacga
     3481 tccggttttg aattttcagt tgtccaacaa ttttcacccc atacgcattc tcaagaatta
     3541 cctcgaaggc gatcaggcca gcgaggaata cgccgttttg ctcaaatggg acaatattta
     3601 ttacgaagag ccggtaagca aggagattgc cagtccctcg atcgtcaaga gttctgcccg
     3661 tctcggactg attcagtggc aaatgcgtct ctacagagac atcgacgact taatgcaaca
     3721 ggctgaatat tttattgatg ccgtttcgag ttaccgggcc gatttcgcac tctttcccga
     3781 gtttttcaat gctcctctga tggcggcttt caatcacctg gaagaacctc aggccatcag
     3841 gaaactagcc agtttcacca aggaaatcgt aaagagattt gcggaatttt ccatttcgta
     3901 caacatcaat attatcacgg gcagtatgcc cgaactggtc gacgataaac tctacaacgt
     3961 gggttatctt tgtaaacgcg acggctcggt ggaacgttac gaaaaaattc atgtcacgcc
     4021 cgacgaggcc aaagtctggg gcatggtggg cggtaatcag cttaagacct tcgacaccga
     4081 ctgcggcaaa atcggcgttt tgatctgcta cgactctgaa ttccctgagc tcagccgcct
     4141 cctggccgat gaaggaatga atattttgtt tgttcccttc ctcacagata cccaaaacgg
     4201 ctattcgaga atcaggaatt gttctcaggc ccgagccatc gaaaacgaat gctacgtagc
     4261 catcgccggt agcgtgggta atttgcccaa agtaaacaat atggatattc aatatgctca
     4321 gtctatggtg tttactcctt gcgattttgc ctttccggtg aacggcatca aagccgaagc
     4381 cacacccaat actgaaatga tcctgattgt agacgtagat ctcaatttac tgaccgagct
     4441 gcacaacaac ggcagcgtac gcaacctgat ggaccgtcgc aaagacatct atgaactgaa
     4501 acgggtttca aactaagatg gggagacaaa gggataaagc tgtgaaaata ggcggcaaac
     4561 agcaactggt ttcgaactta gatggggaga caaaggtaac gtcggcttac gaatttatga
     4621 gcaaggttct gaaagtttct aaataagcct gggaaataaa gcccaaaaat cagttgtata
     4681 cgcttagatg atgaataaaa aatatatatc tttgcgatcg tttcttcaaa tatgtaatta
     4741 tcccgtaaaa taaaaacata aactaaaaac tagaaaagag tatgaaaatc aagaaagtag
     4801 aagccattga aattttggat tccagaggca atcccaccgt agaggttgat cttaaattgg
     4861 aagacggtag ttgcggcaga gcaatggtgc ccagcggtgc ctccaccggc gaacgcgaag
     4921 ccaccgaatt gcgcgacggc gacaaaaaac gctatttcgg caaaggtgta ctcaaggcag
     4981 ttgacaatgt caacaacatc atagccaaag aactcgaagg caaatgtttt gccggacaac
     5041 gcgctgtaga ttaccgtatg ctggaacttg acggaacccc cttcaaatca aaactgggtg
     5101 caaacgccat tctggccgta tcgatggcct ttgcccgtgc agcagctaaa agcagcaatt
     5161 tacctctgta tcaatacctt ggtggaagca atgcttacct ccttcccgtt ccctgtatga
     5221 acatcatcaa tggcggacaa catgccgaca ataatgttga ttttcaggaa ttcatgattg
     5281 ccccccacgg agctacttct tttagggaat ctatccgcat gggcgttgag gttttccaca
     5341 ccctcaaatc tgtcctcaac aaaaagggat acagcaccgg cgttggagac gaaggcggct
     5401 ttgctcccaa cctcaagtcc aacgaagaag ctgtagacgt tattctcgaa gctatcacca
     5461 aagccggttt caaacccgga gccgaagtaa gcatttgtct cgatcccgcc gccagcgaac
     5521 tctgggaaga cggtaagtac aagatgctca agagcagcgg caaatcactg agcagcgatg
     5581 aactggtagc actttgggcc gattgggtaa gacaatatcc catcatctcc atcgaagacg
     5641 gtttggccga aaacgactgg gccggttgga aaaaactgac cgacaccctc ggcaataaaa
     5701 tcgaattggt aggcgacgac ttgttctgca ccaacaaagn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnna ccctcggcaa taaaatcgaa ttggtaggcg acgacttgtt
     5881 ctgcaccaac aaagccatcc tcaaagaagg catcgaaaag ggtgtagcca actccatcct
     5941 gataaaactc aaccagatcg gaacggttac cgaaactctc gaaaccatcg aactggccga
     6001 caaaaactct tacaacaact ttacctcgca ccgcagcggc gaaaccgtcg acagctttat
     6061 tgccgacctg agtgtagccg tcaacgccgg acacatcaaa accggtagcg gttgccgtgg
     6121 cgaacgcatc gaaaaattca accagcttat ccgcatcgaa aaagaattgg gtaccgccgg
     6181 tcgttttgca ggcattgctg cctttaagaa tgctaaataa ttagttgtac ggaaataaga
     6241 aggaatcctt aaactgaaac aagccgggtg attattcatc cggcttgttt gttttggcag
     6301 taaaaagcaa tttatttcct aatgtacttc accagccacc tgcccacagc tgcggttgaa
     6361 cgtgccttcc ggaagatccc ggtggtgatg tcttcggtta cataacctgc gtccatggcc
     6421 acggtaactg cttctcgtat caacttaccc tcttcgctca ggccaaaagt ctgttcaaac
     6481 atcatggctg ccgacaacac ggcggcaaaa ggattggcta tatttttgcc tgctgcctgc
     6541 ggataagaac cgtgtatagg ctcatacacc gaagtatgca aacctatgga agccgagggc
     6601 aacataccca atgagccggt aatgactgaa gcttcgtcgg tgagaatgtc gccaaacata
     6661 ttttcggtaa caataacatc gaaactgcgc ggccattgaa taatacgcat ggcagcgttg
     6721 tccacgtaga gatattccac ttcgatataa ggatattgag gtgccatttc ctgggctatc
     6781 tgtctccaga ggcgcgaggt ctccaacaca ttggccttgt cgaccacggt gagtttcttg
     6841 cgacgtttgg aggcaaattc aaagcccagt ttcaggatgc gttctatttc ttcgcgactg
     6901 taggtacagg tatctacggc gctgttgccg tcttcgctgc gtccctgggg acgtccgaaa
     6961 taaataccgc cggtgagttc gcggatgcat agaaaatcga cattacgaac caatctgttt
     7021 ttgaggggcg aacggctgat caaactggga aaaaccgaaa ccggacgaat gttggcatag
     7081 agtcccagtt tttttcgcat tgacagcaag ccctgttcgg ggcgaatttt ggctttggga
     7141 tcgttgtcgt atttgagtga acccacggca ccaaacagca cggcatcgga atccatacag
     7201 agtttatggg tttcttcggg ataaggatca ccgcaagcat ctatggcggc agcgcccacc
     7261 agacctgttt tataattcaa ttcatggccg aatttttcac aaacggcttt ggtgacgttc
     7321 aaggcctgtt ccacaatttc ggggcctatc ccgtcgccgg gtaatactgc aatgttcagt
     7381 ttcatgtttt tattgtttta ttgttttatt cttgtttctg ttctttaata aagaaaggat
     7441 tttaaaaaaa ccggtccaag actttgaatg ggtttacata ttcatttcct caataatatt
     7501 gagcattttc atggttgctt tgatggcagc ttcggtttgg tctgcgtcca gcccgctggt
     7561 tctgaagctc ttgttattgt acgaccagaa gataacggtt tgtacaaagg catcggtgcg
     7621 tccgccgggc ggtatggtta caacgtaatt taccaactgg ggcaaattgc gtttttggct
     7681 gctgtagatc ttgtgcaggg ctttcataaa ggcgtcgtac tgtccgtcgc cgctggctgt
     7741 ttcctgaaaa acctcaccgc aaatatccaa cataacggtg gctaccggac gcaagccatg
     7801 cgccagcgaa agagcgtaat taagcacttt gacaggctct tcctggtgat tttgcttcag
     7861 cacgtctgat atgatgtaag gtagatcctc ttgagttaca agctcttttt tgtcgcccag
     7921 ttgaatgatg cgttcggtaa ccaaacgcat agcgtcgtcg tcgagctcta tacccagagc
     7981 ttccaggttt tttttgatgc tcgctttgcc ggaggtttta cccaaggcat actggcgtac
     8041 acggccaaaa cgttcgggca gcagatcgtt gaaataaaga ttgcttttga cgtctccatc
     8101 ggcgtgaatg cccgctgtct gggtaaaaac attgtcgccg attaccggct tgttggcagg
     8161 aatgcgcacg ccggaataag attccacaat gcggcttact ctgttgaccc tatcctccct
     8221 gatgccggtt tgaactttca gcaaatcgtg caacacggca ataacactgg taatgggagc
     8281 attaccagct ctttcgccca aaccgtttac cgtagtgtgc acggtagtta ctcctgccct
     8341 gacagcctga aaaacattgg aaacggccaa atcgtaatcg ttgtgggcat ggaaatcaaa
     8401 tttcaatccg gggtaacgtt ccaccatttg ccggcagtaa agggatgttt gttcaggagt
     8461 gaggataccc agggtatcgg gcaacataaa gtgcccaatg gtttcatcgc gcaagctgtc
     8521 catcaagtaa aagacatagt cgggcgaatg catcatgccg ttggaccagt cttcgagata
     8581 gaggttcact tgcaccccct tggctctaca cagggctact tcgtctttga tatcctttaa
     8641 gtgttgttca ggagtttttt tcaattgctc agtaacatgc cggtatgaac ctttggaaag
     8701 caagttgatc accctgcaac cggcatccga ggcccattgc aaggacaagc ctttatctat
     8761 aaagcccaga atttcaatac gatcgagcaa accacgctca ccggcccatt ccgagatacg
     8821 ccgcacagct ttgaattcac cctctgagac tctggcagaa gccacttcta tacgatcaac
     8881 tttgagctct tcgagcaaca gcctggcaat gcttaatttt tcctgcagtc caaaacttac
     8941 acccgaagtc tgttcgccgt ctctcaacgt agtatctaaa atttcaatcg ttttcatttt
     9001 cccaggcttg aatcttgttt ttgttactca gcaaaaagtc gatgtcgtcg tagccttgca
     9061 gcagacaatt ctttttgtag gcgttgatac tgaatgattc ttcgcgaccg ttggcattgt
     9121 tgcgaatgac ttgcttttcc agatccaccg taagggtgct tttcgcatcg gcatagacgg
     9181 tatcgaaaat ttcctgaaga aaagcctcgc tcaccactac gggcaaaact ccgctgttga
     9241 gggcattgtt tttgaaaata tcggcaaaaa agcttgatac caccacacga aagccgtaac
     9301 cggccaatgc ccaggctgcg tgttcgcgac tggaaccgct gccgaagttt ttgccggcca
     9361 ccaatatcga acctccgtaa agcggattgt tcaacacaaa atccggattg ggtcgattgt
     9421 tgccgtcgta acgccaatct ctgaaaagat tatcgccaaa gccttctttg gaagtagctt
     9481 tcagaaaacg ggcaggtatg atttgatcgg tatccacatt ttccatgggc agtggaacga
     9541 cggtggaagt aagtgtagta aatttttcca tgattcaata agttctggga tcggtaatgc
     9601 ggccggtgac ggctgctgcc gccgcaacca gggggcttgc cagtatggtt ctggctccgg
     9661 gtccctgacg gccttcgaaa ttgcggttgg aggtggaaac agcgtatttg ccggcgggaa
     9721 tcttatcgtc gttcatagcc agacaggccg aacaaccggg ttgcctaagg gcaaaaccgg
     9781 cttgctccag aatttcggtc aaaccctctt cgcggcattg tcgctctacc gctttggagc
     9841 cgggtaccag ccaggccaca acaccgggag ctttttgacg tccacggacg aattgggtaa
     9901 aagttctgaa atcttctatt ctgccgttgg tacaactgcc cagaaagacg taatcgatct
     9961 ttttccccaa caaagtttct cccggcttaa aacccatgta gtccaaagct ttgacaaaag
    10021 aggccctgcc agccggatcg atgctttcca acaagggaat acgcccggta atagccatcc
    10081 ccatacccgg gttggtacca taggtaatca tgggctctat atcttcggct ttgaattgct
    10141 cctctttatc gaaaacggcg tcctcgtcgc tgtagagcgt tttccatttc tctactgctt
    10201 gctgccaggc cgctcccttg ggagcaaatt cccggccttt gatgtaatcg aaagtggttt
    10261 gatcgggagc aatcataccg cccctggctc ccatttcgat ggacaggttg caaagcgtaa
    10321 ggcgaccctc catgctcaaa gcccgcacgg cctctccggc gtattccaca aaataaccgg
    10381 tggcacctcc ggtggtcatt tttgcaatga gatacaaggc catatctttg gcgcttacgc
    10441 ccggacggag tttaccctct accctgatgc gcattttctt tggcttagtc tgcaagatgc
    10501 tttgagacgc catcaccatc tctacttcgg aagttcctat gccaaaggca actgctccca
    10561 aagcgccgtg agtggaagta tgcgaatctc cgcagactat ggtcatgcct ggcaaagtaa
    10621 gaccagtttc aggccctacc acatgaatca caccgttttt gggatgtccc agcccaaaat
    10681 acttaatacc gaattcccgg gcgttttcct gcaaggtctc tacctgcttg cgcgaaacag
    10741 gatccttaat aggatggtcc tgattcaggg taggaatatt gtgatcgggg atggcgtagg
    10801 ttttttcggg acgcaaaaag cgcagctttc tgtctctcaa accctgaaag gcctgggggc
    10861 tggtaacttc gtggcaataa tgccggtcga tgtacaattg ggtaggcccg tcgggcacag
    10921 tagtgattac gtgcctgtcc catattttat cgaataaggt ttttcccatg ctcgtattat
    10981 tttcagtcta caaatttatt gatggcgtcg atataagctt cgatagaggc ggtaattatg
    11041 tcggtattac cagaaaatcc gtaatagaca gagccctggt gttctacctg catgtgtacc
    11101 ttaccggtgt cgtcgctgcc cttggctatg gcctgaatca ggaattcctg cagggtcatt
    11161 tcccggttta ttatgcgttt cagtgccttg attccggcgt ccaccggtcc gttgcccgaa
    11221 gcagtggctt caaatttttc accggcaata tccagggtta tgctggccac cgctcttaca
    11281 cccttgcccg aaaccacctg gagatgatcg agtttgatgc gttttttctc gcttctctcc
    11341 tcgcctgtca gcagtaaaat atcctcgtcg ttgatgactt tttttcgatc ggctaattta
    11401 agaaattcct gataaacttc ttgcaagcgt tcctctccaa tctctacacc caaaaggtgt
    11461 aagcggtgtt tcagcgccgc acgtccgctg cgggcggtca acacgatgga gttttcgtct
    11521 atgcccacgt ctttggggtc catgatttcg taattttcgc ggtttttcaa caccccgtcc
    11581 tgatgaatac ccgaggaatg agcaaaagca ttgcggccca ccacggcttt gttgggctgc
    11641 accggcatat tcatcaggct cgataccagg cggctgctgt gcataatatg ctgggtgttg
    11701 atgtcggtat gcacgcccag gtttttgtga cagcgaagta tcatggctat ttcttcgaga
    11761 gaagtattgc cggccctctc gccaacgccg ttgacggcga cttccacctg acgggcgcca
    11821 tttagtatgc ccgacatggt gttggctgtt gccatgccca ggtcgttgtg acaatgagtt
    11881 gaaataacag ccttgtggat attgggcaca ttttccatca aataacggat tttggctccg
    11941 tacacatcgg gcaaacaata accggtggtg tcgggaatat ttacaaccgt ggctccggct
    12001 ttaataacag cttctaccac acgggccagg tattcgttat cggtacggcc ggcatcttcg
    12061 gcataaaact ccacgtcttc gacgtacttc ttggcgtatt taacgcaagc gatagccctt
    12121 tgtaaaatct cgtcgcggtt ggagttgaat ttgtatttga tgtggtaatc cgacgtgccg
    12181 ataccggtgt gaatacgctt tctcttggcg tatttaagag cctcggcagc cacatcgatg
    12241 tctttttcga ccgaacgggt cagagcacaa atggtcggcc aggtaacagc cttggaaatt
    12301 tccaccaccg aattaaaatc gcccggactc gataccggaa aacccgcctc aataacgtcc
    12361 acgcccaaag actcgagggc tttggccacc tgaatctttt ctaccgtatt caactgacaa
    12421 cccggcacct gctcgccatc gcgcagggtg gtatcgaaaa taaaaacttt gtctgacata
    12481 tccattagca ttaaaattag ttcataaaaa aagctcctcc tgcctggtag caagaagagc
    12541 tctatatttt tcgatcgtcg ttctccgata tatacacctg tctcactacc tacctgccga
    12601 acagtagtat agcaattaga atcagggaaa tcagagccgt atggatcata gttttgttta
    12661 ctttcggccg caaatatagc tattttttat
//
LOCUS       DAOSTD010000051        12454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_5079_length_12454_cov_10.323010, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000051 DAOSTD010000000
VERSION     DAOSTD010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12454
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5079_length_12454_cov_10.323010"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1753)
                     /locus_tag="PLI37_04765"
     CDS             complement(<1..1753)
                     /locus_tag="PLI37_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HOQ58356.1"
                     /translation="MTYLKFDKALMVNLQQSLTKELLQTNKSGAYSYSTIVDCNTRKY
                     HGLLVIPVPELDGKNHVLVSSLDTTVIQHGAEFNLGLHKYQGDNYSPQGHKYIREFEC
                     DSNPSTIYRVGGVILSKEKIFVSHENRILIKYTLLEAHSPTKLRLRPFLAFRSINDLC
                     KENNYVNKDYQPVDNGIKMCLYHGYPELHMQLSAKNEFHYSPDWYRNIEYLKEQERGY
                     DFKEDLFVPGYFELPIKKNESVYFSAGISAIPPATMQKMFDEEKSIRTPRNSFTNSLK
                     NAAYQFYNKHPDGQRYLLAGYPWFECRARDMFVALPGCTLAAEDPGLFDAIMETGEKG
                     LRNYMEHKAAGCAITEIDAPDVLLWMIWAVQQYAKDQGVEKCAGKYNKLLFDALDFII
                     GQKHEKLILHSNGLLFTNGKEQPVSWMNSTMYGKPLVPRTGYLVEFNALWYNALCFAS
                     EMAAQVGRAELQTKYSELAQKAGTSFVEVFRNDYGYLYDFVDGQAVDWSVRPNQIFAV
                     SLDFSPLDKMQKKQVLDIVTKELLTPKGLRTLSPKSLGYNPIFQGSESRRTWAYHQGT
                     AWAWLIGPYVEAYLKVHK"
     gene            2024..3016
                     /locus_tag="PLI37_04770"
     CDS             2024..3016
                     /locus_tag="PLI37_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58357.1"
                     /translation="MEKRQKELNLSELLVLLFKSIGNLIKWLWNLFIQALRLSYRKKW
                     IILGFIVAGLVWGFFASTPDKQIHKASITLKTIIKGSPYSKDFIKTLNADCGKKDKSS
                     LAKKLNVSEEEAEKIVSLKSYYLVDIGADGILDQVDYKESFKGDTINIWSNNFLYVEI
                     LSKDENLFPAIREGIVYALNNDPSVQKEKAFMDLEHSNWVSMLDEELEKVKELQSKVF
                     GEDVNNVTSLSLRQKPKQQWSDPNIMTVLGETKVQMFAGDKIHSMVIMNNHKRAIKEL
                     DEGAIRVELPILYLGVTNPRYKVMIIRAFLCYLLGLGLCTYLENRKKIIAFLEA"
     gene            complement(3105..4856)
                     /locus_tag="PLI37_04775"
     CDS             complement(3105..4856)
                     /locus_tag="PLI37_04775"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOQ58358.1"
                     /translation="MENRQLIKEVTAKAKRWLTEDYDAQTRAAVQAMLDKEDKTELIE
                     SFYKDLEFGTGGLRGIMGPGSNRVNQYTVGAATQGLANYLNKSFAHLPRISVVIGHDC
                     RNNSRFFAEICADVFSANGIYVYLFDDLRPTPEVSFAIRHLKCQSGVILTASHNPKEY
                     NGYKAYWDDGAQVVPPHDNNIIDEVQAIKDISSVRFEGNKELIKLIGAEIDEKYIEQI
                     KTISLSPEAIRKHEDLKIVYTPIHGTGVRIIPACLRAFGFKNIINVPEQDVVSGDFPT
                     VVSPNPEEPAALSLALKRAAEVDADIVMASDPDSDRLGIAIKNDREEWMLVNGHQTAL
                     IFIYYLIKRRSELGMLESGKEFIVKTIVTTELIKEIADRHKVDMFDTYTGFKWIAAII
                     REFEGKRQYIGGGEESYGFLPQDFVRDKDAVSSCALMAEILAWAKEQGKSLYELLLDI
                     YIEYGLSREKGISVVKKGKSGAEQIKSMMDNFRSKPVNELAGSPVILIKDYMSLKAYK
                     PESGESFDLKMPETSNVLQYFSHDGTKVSIRPSGTEPKIKFYIEVRDVLKSYSEYPAK
                     CELAEDKFKRIMKSLGL"
     gene            complement(4869..6674)
                     /locus_tag="PLI37_04780"
     CDS             complement(4869..6674)
                     /locus_tag="PLI37_04780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ58359.1"
                     /translation="MKRLASLVLSACVAFILNAQKDKADLLEQATLFVQSYRLQDSIS
                     PSYFDLPLRIPPYLSANFGELREDHFHSGLDFKTQGVINKPVYAVAPAFVSRVSVRVK
                     GFGKAVYLDHPNGYTSVYGHLESFSPRLDSIVKAAQYAAESYEQNLLFPDEQIPVERG
                     ELIGYSGNTGSSGGPHLHFEIRQTESEDPVDPMFWYRDRIKDTSPPKMLALAIYAIDG
                     RGILLNHNGQLSAKEIIQPGQGGAAAPDSKLPLVWGKIGLGIKAYNYMNGTNNLYGLS
                     RIRLFQDGEEIFQQDIGRFSFDESRYLNALVDYGEWVQNRSWIMKSFVEPFNKLDIYP
                     VRINNGYIDINQEREYDFLYELYDISGNKAECRFQLQGKQLPFPQEDTTVLIFYPAYK
                     NTYTSKDVYLEFPSASFYNKLAFEYDYISPKNVKNPGLRNHRLYTGIHKIHHPTVPLH
                     NAAFIRLKISDDVLRDKNRYYLARLLPDNKTEYVEATYTEGFMEGEIPYFGKYAVLAD
                     SLPPKIFHPQLEKNQNNTLIRILVRDDESGLDTYRCTINAKWVLFEYDYKTNTLTADL
                     KDERIGLLPVGNLLQIYVKDKCGNENYREWMFNAE"
     gene            complement(6677..7030)
                     /locus_tag="PLI37_04785"
     CDS             complement(6677..7030)
                     /locus_tag="PLI37_04785"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOQ58360.1"
                     /translation="MLIEVLKSKIHRVRVTEANLDYIGSITIDEDLIEAANLIPNEKV
                     TVLNNNNGERFETYVIKGERGSGVICLNGAAARRALPGDVVIILSYALMDAEEARTFE
                     PCIIFPDTATNRLIK"
     gene            complement(7020..7871)
                     /gene="panC"
                     /locus_tag="PLI37_04790"
     CDS             complement(7020..7871)
                     /gene="panC"
                     /locus_tag="PLI37_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOQ58361.1"
                     /translation="MQVVKTKNQLRTILNAERSKGRSVGLVPTMGALHQGHISLVERC
                     VQENDCCVCSVFVNPTQFNDKNDLIKYPRRPEKDAEVLQKAGCNLLFMPDEQEMYPEE
                     DTRLFYFGPLEEVMEGVYRKGHFNGVAQIVSKLFEAVQPDKAYFGQKDFQQLVIVRKM
                     VEQLNMKVQIVSCPIVREESGLAMSSRNELLTAEQKRKAALISKYLFESLTFVPKSRP
                     AEIREFVLKGISAEPMLRPDYFEIVDGTTLQSIHEWADSACVVGCIAVYCGSVRLIDN
                     IVYKNAD"
     gene            8026..8838
                     /locus_tag="PLI37_04795"
     CDS             8026..8838
                     /locus_tag="PLI37_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOQ58362.1"
                     /translation="MKPAKVLFICQEIMPYVPETELSSICRRLPQAVQDNGNEIRTFM
                     PKYGCINERRNQLHEVIRLSGMNLIIDDNDHPLIIKVASIQAARMQVYFIDNDDFFQR
                     KALVCDESGIDFFDNDERSIFFVRGVIETVRKLRWTPDIVHCHGWFSALAPLYIKKAF
                     DDDPYFRDAKIIYSIYDDAFDSSFRKLFNNKLLVKGINQEDIEEIKAKADYVSLSKLA
                     IRHADGIIQASQKINGKVKKAAEESGKPFMVANESEDFSDYSEFYDLLLEQA"
     gene            8840..10192
                     /locus_tag="PLI37_04800"
     CDS             8840..10192
                     /locus_tag="PLI37_04800"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HOQ58363.1"
                     /translation="MPKHFIHGVLITLLFTALSCTEGQYTGGSIQPPGDIVSTDTASF
                     FLTTETVKVDSILYKNSVALLGEFTDAFFGTTRAEFLAQVYCPLNFTFDASIRRIDSA
                     YLYLYYNDWFGDSLALMEMSVYELTKAIDHTKPYYTNVNTDDYCDKTKKLGSVSYSTG
                     EPFTDIKTSSDYSCIRVNIDTAFAGGILRKNRNHPEFFTNPRVFADSVLHGIAVSPSF
                     GNGNLMYIKHAEFEFCYRYSYTDTMGVVKDTTGACYFPVTKEVRQINKYQHPDLAGYL
                     PLNSGDSLNYIFSPAGLFTRIELPIEEILSRLEGKNINSARIKAVVTHLDNSKWGMLP
                     PDNLLLLKEEDIHDFFAGYNNADKLYSFLADYNTRNDLFSFDLSSFLQKTVRNDSSNF
                     KPYNKLLMVPVSKIRLNNVSTSTLFLVQNILPNAAKIRSATHPQKPMKLEIVYSNKLE
                     "
     gene            complement(10195..10662)
                     /locus_tag="PLI37_04805"
     CDS             complement(10195..10662)
                     /locus_tag="PLI37_04805"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOQ58364.1"
                     /translation="MYKKEIKTNITFCSIDELSKQEKELIKAAKRASLEAYAPYSGFK
                     VGSALLLDNGEILAANNQENAAYPSGLCAERVVLFYANAAHSQHRVLSLAVAASKEGM
                     FTENPISPCGSCRQALLESERRQNTAIRLLLSGEKEVAVVESVASLLPLSFNL"
     gene            10705..11871
                     /locus_tag="PLI37_04810"
     CDS             10705..11871
                     /locus_tag="PLI37_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOQ58365.1"
                     /translation="MSRITLLTLFILSFLYLPLQAQERLKPYEDYIQKYTPLAMTQQI
                     VYGIPASITLAQGLLESGAGNSTLAKNSNNHFGIKCHNDWKGKTYSHFDDGEMSCFRK
                     YSKVEESYKDHSIFLRERARYAFLFELNVKNYKAWAKGLKKAGYATDRHYDKKLIKII
                     EQYDLNKIDKLALNKRRAKKLIRNWEKEYPEEIAKAEKSSVQTESKDRTPKEKSPKVE
                     KSSKKQEPAFGKKSQAAEKAKDYSYIAETSRERQIQRNERNERTDNLPHVINAVQNHT
                     ILYKGLTPYIIAQYGDNFDNISDEFGLNPQRLKNWNEWPPRYKISVGDIVFLSKKAQS
                     WDGEDGIYVVREGDSMHSISQQFGLRVSAIYQLNGMEFGESLEVGQELKLRAQE"
     gene            11929..>12454
                     /locus_tag="PLI37_04815"
     CDS             11929..>12454
                     /locus_tag="PLI37_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816342.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOQ58366.1"
                     /translation="MQEKIVVLDFGSKTTQLIARRIRELKAFCEIYPYNKFPENTDGI
                     KGIILSGSPYSVYDKNAFDFDLTLVRKKFPLLGICYGAQWIVHKAGGKIEASKNREYG
                     KAFFESVNTNDPLFKGFNPLAPVWMSLGDSITRLPAEFEKIALTADIPVAAFKARGEA
                     TWGVQFHPEALQTEE"
BASE COUNT         3555 a         2855 c         2589 g         3455 t
ORIGIN      
        1 ttttatgaac tttgaggtaa gcttcaacat atggaccgat cagccaggcc caggccgtcc
       61 cctggtgata tgcccaggtc ctgcgacttt cggacccctg gaatattggg ttatacccca
      121 aactcttagg gctgagtgtt cgcaaaccct tgggtgtgag caattccttg gtgacaatgt
      181 cgagtacctg ctttttttgc atcttgtcca atggtgaaaa atcgagagag acagcaaaaa
      241 tctgattcgg gcgcacactc caatctaccg cctgtccgtc cacgaaatcg tacaaataac
      301 cgtaatcatt gcggaatact tctacaaaag aggttccggc tttctgtgca agctccgagt
      361 atttggtctg taattccgct ctgccgacct gagcagccat ttctgaggca aagcaaaggg
      421 cattatacca tagagcgtta aattcaacca aataaccggt tctcggcacc aggggtttgc
      481 cgtacatcgt ggaattcatc caactgacag gttgctcttt gccgttggta aagagcaacc
      541 cgttgctatg caaaataagc ttctcgtgct tttgaccgat tataaaatcc agcgcatcaa
      601 acagaagttt attgtatttt ccggcacatt tttccacacc ctggtctttg gcgtattgct
      661 gaacagccca tatcatccac aacaagacgt ccggtgcatc tatctccgtg atagcacaac
      721 cggctgcttt gtgctccata tagtttcgca aacctttttc accggtttcc attatggcat
      781 caaacaaacc gggatcttcc gcggcaaggg tacaaccggg aagagcaaca aacatgtccc
      841 ttgcccggca ctcaaaccag ggataacctg ccagcaggta gcgctgtccg tcgggatgtt
      901 tgttgtaaaa ctggtaagct gcgtttttca ggctgttggt aaaactgttt ctcggtgtac
      961 gaatcgactt ttcttcatca aacatctttt gcatggttgc aggcgggata gctgaaatgc
     1021 ctgccgaaaa atatacgctt tcgtttttct taatgggtaa ttcaaaatag cccggaacaa
     1081 acaagtcctc tttgaaatca tagcctcttt cctgctcttt caaatattct atgtttctgt
     1141 accagtcggg agaataatga aattcatttt tcgcggacaa ttgcatatgc aactcaggat
     1201 aaccatggta aagacacatt tttattccat tatctacagg ttgataatct ttatttacat
     1261 aattgttttc cttacacagg tcgttgatgc tgcgaaaagc caaaaaaggc ctcaacctga
     1321 gcttggtggg agaatgcgct tcgagtaagg tgtattttat cagtatgcgg ttctcgtgtg
     1381 aaacaaatat tttttccttg gaaagaatta caccccccac cctataaatg gtcgagggat
     1441 tggaatcgca ttcaaactcg cggatgtatt tatgtccctg agggctgtaa ttgtctcctt
     1501 ggtatttgtg caaaccgagg ttgaattctg ctccgtgttg aatgacggtc gtatccagag
     1561 aagaaaccag cacatgattt ttcccgtcca gctcaggaac gggaatcacc aacaaaccgt
     1621 gatatttcct tgtattacag tcgactatgg tggaataact gtaggctccc gatttgttgg
     1681 tttgcagcaa ttctttggtc aacgattgct gcaagttgac cattaaggct ttgtcaaatt
     1741 tcaaataggt cataacagta catgttttgc gtattgcgat taataaactg cattagcagc
     1801 gagtccggcg ataatgcaaa aaaacgatag acgaagataa gcttattctc ggggaattac
     1861 aagaggaaag agcccaaata aaaggcaaca agtatatgtt ttacatcatc ttttaggctg
     1921 ttttggggca gtttgttctt tatggcgggg attttgttga caatagcaga attttagtac
     1981 ttttgcattc tcatttatac atttacttac gatacttcgt actatggaaa aacgacaaaa
     2041 agaactcaat ctttccgaac ttctggtgct tttgttcaaa agtatcggca atttgatcaa
     2101 gtggctgtgg aatcttttta tacaagcttt gcgcttgagc taccgaaaaa aatggattat
     2161 tctgggattt attgtggccg gacttgtatg gggatttttt gcttccacgc ccgacaaaca
     2221 gatccataag gcgtccatca cactaaaaac catcattaaa gggagtccgt acagcaaaga
     2281 ttttatcaaa acattgaatg ccgattgtgg aaaaaaagac aagagcagcc tggcaaaaaa
     2341 gctgaatgtc tccgaagaag aagccgaaaa aattgtttcc ctgaaatctt actatttagt
     2401 tgacatcggt gctgatggta ttcttgacca ggtcgattat aaagagagct ttaaaggtga
     2461 cacaatcaat atctggagca ataacttcct atatgtcgaa attttatcca aagatgaaaa
     2521 cctattcccc gccataagag agggtattgt ctatgccttg aacaatgacc cgtctgtgca
     2581 aaaagagaaa gctttcatgg atttggaaca ttccaactgg gtttcgatgc tcgatgaaga
     2641 attggaaaaa gttaaggagt tacaatcgaa agtatttggt gaagacgtaa acaacgtcac
     2701 ctctctgagt ttaaggcaaa agcccaaaca acaatggtcc gaccccaata taatgacagt
     2761 attgggcgaa acaaaagtgc aaatgtttgc cggtgataaa attcactcta tggtaattat
     2821 gaacaatcat aaacgcgcga ttaaagaact tgacgaagga gccatacgcg tagaattacc
     2881 tattttatac ctgggagtga caaatcccag gtacaaagta atgataatac gggctttcct
     2941 gtgctacctt ttaggcttgg gactttgcac ttacctggaa aaccgtaaaa agatcattgc
     3001 ttttctggaa gcataacggc cttccggact acccgggtca ttttaacgct cacccgaaga
     3061 gttaaggaca actgtagctg gcagatacct gctgaaaaaa ggctctacaa acccaacgat
     3121 ttcattattc tcttgaattt gtcttcggcc agctcacatt tggcggggta ttcagaataa
     3181 gatttcagca catcgcgtac ttcgatgtag aatttaatct tcggttccgt acccgaagga
     3241 cggatggata cttttgttcc gtcgtggctg aagtattgta acacattgga agtttcaggc
     3301 attttaagat caaaactttc tccgctttcc ggcttatagg ctttcagact catgtaatcc
     3361 ttaatcagaa ttaccggaga acctgccagt tcattcaccg gcttggacct gaagttgtcc
     3421 atcatagatt tgatttgttc agcccccgat ttcccttttt taacgacaga aatgcctttc
     3481 tctctcgaaa ggccgtattc tatgtaaata tcgagcaata attcatacaa gcttttccct
     3541 tgttctttgg cccaggcgag tatttcggcc attaaagcac aggacgaaac agcgtcttta
     3601 tcacgaacaa aatcctgagg caggaaacca taactttcct ctcctccgcc tatgtattgc
     3661 cgtttacctt caaattccct gattatagcc gctatccact taaaaccggt gtaggtgtcg
     3721 aacatgtcca ccttgtgcct gtcggcaatt tctttgatca attcggtggt gactatagtt
     3781 ttgacgatga actccttacc actttcgagc atccccagct cggaacgtct tttaatcagg
     3841 tagtagataa agataagggc ggtttgatgt ccgttgacca acatccattc ttcacggtcg
     3901 tttttgatcg ctattccgag acggtcggaa tccggatcgg aagccattac aatatcggca
     3961 tcgacttcgg ctgctctttt cagcgccaac gacaaagctg ccggttcttc tggattgggt
     4021 gaaacaaccg tgggaaagtc accgctgact acatcctgtt cgggcacatt gatgatgttt
     4081 ttgaagccaa aagcccggag gcaggcggga ataattctga caccggtacc gtgaatgggt
     4141 gtatatacta ttttcaggtc ttcatgtttt cgaatagcct cgggcgatag agaaatggtc
     4201 ttgatttgtt caatgtattt ctcatcgatc tcggcgccaa tcaacttaat gagctcctta
     4261 tttccctcga agcgaacgga gctgatatcc ttgatagcct gtacttcgtc aattatatta
     4321 ttatcgtgag gtggcaccac ctgggccccg tcgtcccaat aagctttata accgttgtat
     4381 tccttggggt tgtgcgaagc cgtcaaaata actccgctct ggcatttcag atgacgtatg
     4441 gcaaaagaca cttcgggagt gggacgtaaa tcgtcgaata aatacacata aatgccgttg
     4501 gccgaaaata cgtcggcaca gatttctgca aagaagcggc tgttgtttct gcaatcgtgc
     4561 ccaatcacca cagatatccg gggtaagtga gcaaaagatt tattcaggta attggccaag
     4621 ccctgagtgg cggctccaac cgtgtactga ttcactctgt tgctgccggg tcccattatt
     4681 ccacgtagcc cgccggtacc gaattccaga tctttgtaaa aagattcaat taattctgtt
     4741 ttatcctcct tatcgagcat agcttgaact gcagccctgg tttgcgcatc gtaatcctct
     4801 gtcagccaac gcttggcttt ggctgtgact tcttttatca actgtctgtt ttccatagct
     4861 tatttgtatt attctgcgtt aaacatccac tccctgtaat tttcattgcc acacttatcc
     4921 tttacataaa tctgcaaaag attgcccacc ggcaacaaac caatccgctc gtcttttaaa
     4981 tcggccgtca aggtattggt cttgtaatcg tattcaaaca aaacccactt agcgttgata
     5041 gtacagcggt aagtgtctaa gcccgattca tcgtcacgga caagtatgcg aattaaagta
     5101 ttattttggt tcttttcaag ttgaggatga aaaatttttg gaggcagact gtcggctaat
     5161 acggcatatt tcccaaaata aggaatttct ccttccataa agccctctgt atatgttgct
     5221 tctacgtatt ctgtcttatt atcgggtaat agtcttgcca gataatagcg attcttatcc
     5281 cttaacacgt cgtcgctgat ctttagacgg ataaatgccg cattgtgcag cggaacagtg
     5341 ggatggtgaa ttttgtgtat gcccgtgtac aaacgatgat ttctgagccc gggatttttg
     5401 acattcttcg gagatatgta atcgtactcg aaagccaact tattgtaaaa cgaagctgac
     5461 ggaaactcca gatacacatc tttggaagta taggtgtttt tgtatgccgg ataaaaaatt
     5521 aaaaccgtgg tatcttcctg aggaaaagga agctgtttgc cctgcaactg aaagcggcat
     5581 tcagctttgt ttcccgatat atcgtacaat tcgtacaaaa aatcgtattc ccgttcttga
     5641 tttatatcaa tatagccgtt gttgatcctg acgggataaa tatcgagctt attgaaaggc
     5701 tctacgaaag atttcataat ccaggaacgg ttttgcaccc actctccgta atcgaccaag
     5761 gcattcaggt agcggctttc gtcgaaagaa aagcgcccta tgtcttgctg aaatatttct
     5821 tcaccgtcct ggaataggcg aatccggctc aaaccgtata gattgttggt gccgttcatg
     5881 taattatagg ctttaatacc caagcctatt ttgccccaga ccaaaggcaa tttgctgtcc
     5941 ggagctgcgg ccccgccctg tccgggttgt attatttctt ttgcagacag ctgtccgttg
     6001 tgatttaaca aaattccccg cccatcgata gcgtagatgg ccagggccaa catttttgga
     6061 gggctggtgt ctttgatgcg atccctatac caaaacatgg gatcgaccgg gtcttcggat
     6121 tccgtttgac gaatttcgaa atgcaaatga gggccgcccg aactgcctgt atttccgcta
     6181 tatcctatca actcgccgcg ttcaacgggt atttgttcat cgggaaacaa taaattctgc
     6241 tcataagact cagccgcata ttgggccgct ttgactatag aatccaacct tggagaaaaa
     6301 gattctaaat gcccgtaaac actggtataa ccattgggat gatctaaata aacagccttt
     6361 ccaaatcctt ttacacgtac gctgacacgc gaaacaaaag ccggagctac agcgtatacg
     6421 ggtttattaa ttacaccctg agttttaaaa tccaggccgg aatgaaaatg atcctcgcgc
     6481 agttctccga aattggcact gaggtaggga ggaatacgta agggtaaatc gaaataagaa
     6541 ggtgaaatac tgtcctgcaa cctgtatgac tgcacaaaca aagtagcctg ctccaacaaa
     6601 tcagccttgt ctttttgcgc attaagaata aaagcaacac aagccgacaa cactaatgat
     6661 gcaagtcgct tcattctcac ttaatcagtc tgttggtcgc ggtgtcggga aaaataatac
     6721 agggttcaaa tgtgcgtgcc tcttccgcat ccatcaaagc atacgacagt ataatcacga
     6781 catcgccggg tagagcccta cgggccgcag ctccattaag acaaataacg ccggagcctc
     6841 tctccccttt gataacatat gtttcgaaac gttctccgtt attgttgttc aataccgtaa
     6901 ccttttcgtt gggaataagg ttggcagcct caatcaggtc ttcgtcgatg gtaatgctac
     6961 cgatgtaatc caggttggct tccgtcacac gcaccctgtg aatttttgat tttaggacct
     7021 caatcagcat ttttatatac tatgttatct attaaacgca ctgacccgca atataccgca
     7081 atacatccaa ctacgcaggc ggaatcggcc cattcatgta tgctttgcag ggtggttccg
     7141 tcgacaattt cgaaataatc cgggcgaagc ataggttcgg cactgatccc cttaagcaca
     7201 aactccctga tttccgccgg cctgctcttc ggtacaaagg taaggctttc aaataaatat
     7261 ttggatataa gggccgcttt tcttttttgc tccgccgtta aaagttcatt gcggctgctc
     7321 atggccaatc cgctttcttc acgtacaatg ggacaagata cgatttgaac tttcatattc
     7381 agttgttcga ccattttacg cactatgacc aattgttgaa aatctttttg cccgaaatag
     7441 gctttatcgg gttgaactgc ctcaaaaagc ttacttacaa tttgagcgac tccgttgaaa
     7501 tgcccctttc tgtatactcc ctccatcacc tcttcgagag gaccgaaata aaaaaggcgg
     7561 gtgtcttctt cggggtacat ttcctgttca tcgggcataa ataacaggtt gcaaccggct
     7621 ttttgcaaca cttcggcgtc tttttcgggt cgtctggggt atttgatcaa atcgttttta
     7681 tcgttaaact gcgtgggatt tacaaagaca ctgcaaacac aacaatcgtt ctcctgaaca
     7741 caacgttcta ccagggaaat atgtccctga tgtaaagcac ccattgtggg aacgagccct
     7801 acactacggc ctttgctgcg ttctgcattt aaaattgttc ttagttggtt tttagttttt
     7861 actacctgca taaacatctg aaagtttggt cgatttcccc cttgtaaaga gggcgcaaaa
     7921 aaaccaattt tttggcattt aaaaaaataa aacgctcatt tcttgccttt ccagctattt
     7981 tttttagtat ctttgcaggc atatgttcac aatcctcatg tttttatgaa accggcgaaa
     8041 gtactgttta tctgtcaaga aataatgcct tatgttcctg aaacggaact ttcgtccatc
     8101 tgccgccgtt tacctcaagc tgtccaggat aatggcaatg aaatcaggac ttttatgccc
     8161 aaatacggct gcattaacga acgcagaaac caattacacg aagtaatcag gttgtcgggg
     8221 atgaacctga ttatcgacga caacgatcat ccgctgatta tcaaagtggc ttctatccaa
     8281 gccgcccgta tgcaagttta ttttatagat aacgacgatt tttttcaacg caaagccttg
     8341 gtttgtgatg aatcgggcat cgattttttt gataacgacg aaaggtcgat tttttttgta
     8401 cgcggggtca tcgaaacagt ccgaaaattg cgttggacac ccgacattgt acattgtcac
     8461 ggatggtttt cggccctggc tcccctctac atcaaaaaag ccttcgacga tgacccctat
     8521 ttcagggacg ccaaaataat ttattctatc tacgacgatg ctttcgatag cagcttccgg
     8581 aaacttttca ataacaagct gctggtaaaa ggcattaatc aggaagacat cgaagaaatc
     8641 aaagctaaag ccgattatgt aagcctgtca aaactagcca ttcgccatgc tgacggcatt
     8701 attcaggcca gccaaaaaat caacggcaaa gtaaaaaaag cagcagaaga gtcggggaag
     8761 cccttcatgg ttgccaacga aagcgaggac ttctctgatt acagcgaatt ttacgacctt
     8821 cttttagaac aagcttaata tgcccaagca tttcattcac ggcgtgttga tcacgctatt
     8881 gtttacagcg ttatcgtgca cggaaggtca gtataccgga ggcagcatac aaccgcccgg
     8941 cgacattgta tcgaccgata cggccagttt ctttttaacc acggaaaccg taaaagtcga
     9001 ttccattctc tacaaaaaca gtgtcgctct tttgggagag ttcaccgatg ctttttttgg
     9061 aactacccgg gcggaatttt tggctcaggt atattgtccc cttaatttta ccttcgatgc
     9121 aagcatccgg cgaatcgatt ctgcttattt gtatttgtat tacaacgatt ggttcggcga
     9181 ttctctcgcc ttgatggaga tgagcgtata tgaactcaca aaagccatag accacacaaa
     9241 gccctattac accaacgtta atacggacga ttactgcgat aaaacgaaaa agttaggttc
     9301 tgtcagctat tctaccggag aaccctttac cgatattaaa acatcgagcg attattcctg
     9361 catccgggtc aatatagata cggcctttgc cggggggata ctccggaaaa acaggaatca
     9421 tccggagttt tttaccaatc cgagagtttt tgccgattca gtgcttcacg gcattgctgt
     9481 cagccccagt ttcgggaacg ggaacctgat gtatatcaaa cacgccgaat ttgaattctg
     9541 ctatcgatac agctacaccg atactatggg tgtggtgaaa gacactaccg gagcgtgcta
     9601 cttcccggta accaaagagg taaggcaaat aaacaagtat cagcaccccg atctggccgg
     9661 ctacctgccc ttgaacagcg gcgactctct gaattatatt ttttcacccg caggcttgtt
     9721 tacacgtatt gaattgccca ttgaagaaat tctttccagg ctggaaggga aaaacatcaa
     9781 cagtgccaga ataaaagcgg tggtcaccca tcttgacaac agcaaatggg ggatgctccc
     9841 acccgacaac ctcttactcc tgaaagaaga agacatccac gatttttttg ccggctacaa
     9901 caacgccgat aaactttact cctttttagc ggactacaat acccggaacg atcttttttc
     9961 ttttgattta agttcctttc tgcaaaaaac cgtacgcaac gacagcagca actttaagcc
    10021 ctataacaaa ttgctgatgg ttcctgtaag caagatcaga ttgaataatg tctcgacaag
    10081 tacgcttttt ttggttcaaa acattttgcc caacgcagcc aagattcgca gcgcaacaca
    10141 tccccaaaaa cccatgaaac ttgagattgt ttacagcaac aaacttgagt aaaattacaa
    10201 attgaaggat aaaggcaata agcttgcgac cgattctacc acagccactt ctttttctcc
    10261 ggaaagcagc aacctgattg ctgtattttg tcgccgttcg ctttcaagta aagcctgcct
    10321 gcaacttcca caggggctga tcggattttc agtgaacatt ccctctttac tggccgctac
    10381 agctaaactg agaactctgt gttgagaatg ggctgcattg gcgtagaaca gcaccacacg
    10441 ttcagcacaa agccccgaag gataggcggc attctcctga ttattggccg ccaatatttc
    10501 gccattgtcg agtaacaaag ccgagcccac tttaaacccc gaataaggcg cataggcttc
    10561 taaactggcc ctttttgcgg ctttaatcaa ttctttttcc tgcttgctca attcatcaat
    10621 ggaacaaaag gttatatttg tctttatttc ttttttgtac atctgagcag atttttacat
    10681 cttccacaca aaaataccat ttctatgagc agaattacac ttttaacctt atttatttta
    10741 agctttcttt atctcccctt acaggcacag gaacgcctga agccttatga agactatatc
    10801 caaaaataca ccccgctggc catgacacaa caaatcgtat acggcatacc tgccagcata
    10861 acccttgcac agggcctcct ggaatcgggg gcgggcaaca gcactctggc aaaaaacagc
    10921 aataaccact tcgggataaa atgccacaac gactggaaag gaaaaaccta ttcccatttt
    10981 gatgacggag aaatgagttg ctttagaaaa tattccaaag tagaagaatc ctacaaagac
    11041 cactccatat ttttaagaga gagagcgcgt tacgcttttt tgtttgaatt gaatgtaaaa
    11101 aactataaag cctgggccaa aggcctaaaa aaagcaggtt atgccacaga tcggcattac
    11161 gataagaaac tgataaaaat cattgaacaa tacgatttga acaaaataga taaactggca
    11221 ctcaacaaac gacgggcaaa aaagcttatc cgaaactggg aaaaggaata tcctgaagaa
    11281 atcgcgaagg ccgaaaaatc ctccgtccaa acggagtcaa aagatagaac tccaaaagag
    11341 aaatcaccaa aggttgaaaa aagcagtaaa aaacaagaac ccgcattcgg caaaaaaagc
    11401 caagctgccg aaaaagccaa agattacagc tacattgccg aaacgagcag agaaagacaa
    11461 attcagcgca acgaacgcaa cgagcgcacc gacaacctgc cccacgttat taacgccgta
    11521 caaaaccata cgattttata taaaggtctg actccatata tcattgcaca atacggggat
    11581 aacttcgata atatttccga tgaattcggc ttaaaccctc aacgtctaaa aaattggaac
    11641 gaatggcctc ccaggtataa aatttcggtg ggagacattg ttttcctgag taaaaaagct
    11701 cagtcatggg acggcgaaga cggcatctat gtggttcgcg aaggagacag catgcacagt
    11761 atttcacaac agttcggact tcgggtaagc gccatttacc aactcaacgg catggaattt
    11821 ggcgaaagtc tcgaagtagg tcaggaatta aaattgaggg cccaggaata aaaaaaatag
    11881 caaagctttt tatacctttg caccccaaat agtgtcaatt cccccattat gcaagaaaaa
    11941 attgtcgtac tcgatttcgg gtctaagacc actcaactta ttgccaggcg catcagagag
    12001 cttaaagcct tttgtgaaat ctatccttac aataaattcc ccgaaaacac cgatggtatt
    12061 aaagggatca ttctgtcggg tagcccttat tctgtttacg acaaaaacgc cttcgacttt
    12121 gatttaactt tggtaagaaa gaaatttcct ctgttgggta tttgttacgg agcacaatgg
    12181 atagtccaca aagccggggg gaaaatagaa gcttccaaaa acagagaata cggcaaagct
    12241 ttttttgaat cggtcaacac caacgatccc ctgttcaagg gctttaatcc cctggctcct
    12301 gtttggatgt cgctgggaga cagcatcacc cggctgcccg ccgaattcga aaagatcgct
    12361 ttaactgcgg acatcccggt agctgcattc aaggccagag gagaagccac ctggggcgtt
    12421 caatttcacc ccgaggcgct ccaaaccgaa gaag
//
LOCUS       DAOSTD010000052        12172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_5228_length_12172_cov_14.574730, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000052 DAOSTD010000000
VERSION     DAOSTD010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12172
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5228_length_12172_cov_14.574730"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLI37_04820"
     CDS             complement(<1..316)
                     /locus_tag="PLI37_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOQ58367.1"
                     /translation="MHPSEYIKESMEYRFIREHIKEDVRKLALQTHGMKDMNYPFLIR
                     QISGYQHIVNKIPEWYACPDILFPPTLSLEQCSSQQTACYKRDLMKTIIRKQKQEQTI
                     SGA"
     gene            441..3446
                     /locus_tag="PLI37_04825"
     CDS             441..3446
                     /locus_tag="PLI37_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOQ58368.1"
                     /translation="MTAADRLKKLDFKDTSFANLMVKRIFNILLISSKYDFFMLEDDS
                     RVDEQIFNEYISLNLRYPPRFTQVSNKKEALEQLGQNNYELIIYMPSDKSNEHFEIAK
                     QIKHKWPAIPIVVLTPFSREVSQYLANADTSAIDYVFSWLGNSDLLLAIIKLMEDKMN
                     VEEDVASVGVQVIMLVEDSIRSYSSILPNLYKYVLEQSRNFATEALNAHQRTLRMRGR
                     PKILLARSYEEAMEIHDKYKDYMLGIISDVSFMKEGKKTQNAGFLLCNEFRKYDKALP
                     VIINSSEASNRTYAEQNEMGFIQKSSKTYSKELRHLLADTFGFGDFVFKNPQTGEEVA
                     RIQNIQELQSAIFDIPDDSLRYHFERDHASRWLFSRAMFPIARFMKGILIQDYEDTAP
                     VRKMLFDAIVLYRRVKNQGIVAEFQKGRFDKYSNFARIGEGSLGGKGRGLAFLDVMLK
                     SHPELNTYRNVHIGIPKTLVLCTDIFDEFMDNNHLHELAMSDQTDEIILENFLKASLP
                     PRLLENLTAFVDVVQKPIAIRSSSLLEDAHYQPFAGIYSTYMIPYNEHAEDMLIMLKS
                     AIKAVYASAFFQQSKAYMQATSNLIDQEKMAIILEEVCGTAFDERFYPTFSGVARSLN
                     YYPIGEEEAGDGIANVALGLGKYIVDGGTTLRFSPKHPKHLLQMSSLNYALSETQTHF
                     LALDLKNKSFRLKVDDGQNLLELPISEAEKDGNLKFLASTFDPLDQVIRDGLWEGGRK
                     IISFAHILQNDVFPLADILSKILKLGEQEMGRPIEIEFAVDFHPRDEDDSPEQRYFYL
                     LQIRPIVDTKEMLDEDLDEIPEEQLLIKTISALGHGIVDDIYDLIFIKPELFSSSNNQ
                     LCVHEIEKINKQLGDEGRNYILIGPGRWGSSDSWMGIPVRWSQISNARLIVETGLRKY
                     QIDPSQGTHFFQNLTSFGVHYYSLPNSDIAFNYVDYEYIHNHPAFYENQFLKHVRFEN
                     PLVFKTDGKKGKGVLLKANGLSVAFPKMS"
     gene            complement(3476..4333)
                     /locus_tag="PLI37_04830"
     CDS             complement(3476..4333)
                     /locus_tag="PLI37_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOQ58369.1"
                     /translation="MDRKIPDYINEKGNVVRLVVLTAVFALLFINIYEPFGSKNWQGG
                     VSDLTYFLFSNLVILVGMCVVAISRIIMYHYNKNHEISVLLYLAWVVMEVIAMSLFYT
                     LFQYFALNDTRPFLEIWKNATINTSLILLLPYAILWLYFSWRDNEKMLKRIREQESLL
                     AEKQKSGLISFYDDKGVLRLSVDINSLYYIESADNYVKIHYLNKGKMQHFMLRSSLKT
                     IDDLFGDKYLVRCHRSYIVNFDKIKILRKSEEGLSLELDSDLVPDIPVSKTFSNKLMS
                     RFTDLQKGP"
     gene            4538..5659
                     /locus_tag="PLI37_04835"
     CDS             4538..5659
                     /locus_tag="PLI37_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HOQ58370.1"
                     /translation="MNLAQRQEELQRQFKLGIDTKLENSPVEIRHFPYTSLDEDKGEK
                     AGGSDSDILENREFVYPIFKLKSQDKCPGAIFLMHGLNERSWNKYLCWAEYLCMHTGK
                     AVVLLPIAFHINRSPLSWLNPRLISPILEKRRQILGKEQSQSIANTILSERLSEDPKR
                     FYRSGKQSLSDLTGLVRQIKEGRHPLFTSGTGVDFFAYSIGAFLVQIALLSDKEGLLE
                     DSGLFLFCGGSIFKQMNGTSRFIMDKLTFDKLCDYYLYRFESDNRIDQTSEASMIAFN
                     TMIEKERGAEERYRIFNEMGNKIQVMALKKDSVIPYNGIVEALGTECAQRNSSLIDFP
                     YEYSHEQPFPIMKGQKEQNDLVNLCFNGVFSQAAAFLSK"
     gene            complement(5690..7336)
                     /locus_tag="PLI37_04840"
     CDS             complement(5690..7336)
                     /locus_tag="PLI37_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ58371.1"
                     /translation="MSKHFFSFLEDTIKNHWDAPAITDYETDFTMTYGELAEHIAQLH
                     LLFKEMGLQKGEKIAICGRNSVNWAMSFLAANTYEAVVVSILPDFHVDSIHALVNHSE
                     ASLLFVGPSVWENLEAEKMPQLKSIVSLRNFELIQTKSAAVKQTFAIKDQLFGRHYPN
                     GLQMQDISYPKNNFDDLAVINYTSGTTSAPKGVMLTHRSISSNVEFGQARIPNQVGWN
                     IVSMLPLAHMFGLTFEFLYQLAGGCHVYFLNKTPSPQVLMKAFADTNPYMILTVPLVI
                     EKIFKKTVFPVIHKPVMKVLWYTPGINLVLRNAVRKKLMTAFGGKLRYLIIGGAALNF
                     EVENCLKQIRFPYTVGYGMTECGPLLGYEDWRHFAKASCGKPVHRIELRIDSANPYKE
                     AGEIQVKGDNVMLGYYKNDEATEAAFTQDGWMRTGDLGLLDKRGNIYIKGRSKSMILG
                     SSGQNIYPEEIEDKLNNLPLVVESVVVERDEKLVALVYPDFDAAGGESKEEAINEVME
                     QNRLSLNKLLPAFARIMKIELVKKEFEKTPKRSIKRFLYK"
     gene            7655..8227
                     /locus_tag="PLI37_04845"
     CDS             7655..8227
                     /locus_tag="PLI37_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOQ58372.1"
                     /translation="MQLKGSKTEQNLLKAFAGESQAKTRYEFFASVAKKEGYEQISAF
                     FTETALNEKEHAELFFKYLEGGMVEITASYPAGVIGTTAENLKAAADGEHEEWAELYP
                     LFAQIAKEEGFKEISATFTLVAKVEAEHEKRYRKLLQNIVENKVFEDDENTTWTCRNC
                     GYVHKGKNVPDLCPVCKHAKAYFERRCENY"
     gene            complement(8266..>8985)
                     /locus_tag="PLI37_04850"
     CDS             complement(8266..>8985)
                     /locus_tag="PLI37_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOQ58373.1"
                     /translation="YYLAFASTCCPEGIGYAMSDGPEGPWEYKGHIMDHTVRTRGNHP
                     GIISYKGKHYVFGLNYDIMHLTTFAHAEQRSAALAEMHYNPDGTIQEVPYFMDNVVEQ
                     IKPFDPYRRVEAETMSWGYGLKTKRMDEENKNSTNMFVTNIDDGEHIKVSGVKFGNKG
                     AKKFYASVATDSLARIEIRLDSPDGPLAGTLSISPTGGESQYHTVSCKLKPRLRGTHD
                     LYFVFRGTGKNLFNFDWWRFK"
     assembly_gap    8986..9085
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9086..9386)
                     /locus_tag="PLI37_04855"
     CDS             complement(<9086..9386)
                     /locus_tag="PLI37_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007767742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOQ58374.1"
                     /translation="MPIIQTSFTADPAPVVINDTVFLYTTHDEDGARGFQMFDWLLYT
                     SDDMVNWQDHGAVASLDDFKYYDGKNGAWAQEVVEANGAYYMYCPIHGRGIGVLMS"
     gene            complement(9459..10697)
                     /locus_tag="PLI37_04860"
                     /pseudo
     CDS             complement(9459..10697)
                     /locus_tag="PLI37_04860"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
     gene            complement(10808..>12172)
                     /locus_tag="PLI37_04865"
     CDS             complement(10808..>12172)
                     /locus_tag="PLI37_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HOQ58375.1"
                     /translation="NLRTDYEGFYPMRTEARSGSYGYPLLTEYADGLFGLISESDVQH
                     GMSGSHLEVRDDNAYHIKYMSEGKSENGISPAFVLIIGSLADIVESTLVTDVATPCQI
                     EDISWIHPGLSSWIYWAYNRSSKDFGKVKEYIDLAAEMSWPYTLIDWEWDEMAGGGDI
                     EDAMAYAREKGIKVNLWYNSGTSWVGPGAPTPLDRLRTAEARESEMTRLNNMGVSGIK
                     VDFFLPDGAEMIDYYLDILEDAARHRLLVNFHGCTVPRGWNRTWPNLMTMEAVYGAEW
                     YNNTPRMTQAAASHNATLPFTRNVIGPMDYTPCTFSDSQHKHITTDCHELALPILFES
                     GLQHMADRPESYLSLPYQVKELLMGLPSVWDDTRLLAGYPGESAVIARRSRDRWYIAG
                     INGTDEVLELTPDISRLEQQGKVTVFTDRGQEPGQGFEINENVDLPESFILQPRGGFV
                     ILTD"
BASE COUNT         3341 a         2929 c         2640 g         3162 t
ORIGIN      
        1 cggctccgct tatcgtttgt tcttgctttt gcttccgaat tatcgttttc atcaggtctc
       61 ttttgtagca agccgtttgc tgcgaagaac attgctccag cgacaaagtt ggaggaaaga
      121 gaatatcggg gcaggcatac cactccggaa ttttattcac aatatgctga tatccggata
      181 tctgcctaat caagaaggga taattcatat ccttcatgcc gtgggtttgc aaagccagtt
      241 tgcgaacatc ctctttgata tgttcccgga taaaacggta ttccatactc tctttaatgt
      301 attccgaagg gtgcataaac gggttttttg caaatataag gaaatcgtcc ggccaatgcg
      361 acacaaatta aatttgacag ctcaaatcgc tgattcacgc tgtttttact tacatttgca
      421 ttcagtttca aattacaact atgactgctg ccgaccgcct taaaaaattg gattttaaag
      481 atacttcttt tgccaacctg atggtaaaac gtattttcaa cattttgctg atttccagca
      541 agtacgattt ctttatgctc gaagacgaca gcagagtcga cgaacaaatc ttcaacgaat
      601 acatttcact taatctgcgt tatccgccac ggtttaccca ggtgtccaac aaaaaagaag
      661 ctctggaaca actcgggcaa aacaattacg agcttatcat ttatatgccc agcgacaaga
      721 gcaacgaaca ttttgaaatt gccaagcaaa ttaaacataa atggccggct atccctatcg
      781 tagtactgac tcctttttca cgcgaagttt cgcaatacct ggccaatgcc gataccagtg
      841 ccatcgatta tgtattcagt tggttgggca attccgattt gctgctggcc attatcaaac
      901 ttatggaaga taagatgaac gtggaagaag acgttgcctc tgtaggagtg caagtaataa
      961 tgctggtcga agattccatc cgctcctatt cttccatttt gcccaatctt tataaatacg
     1021 tgctggagca atcaagaaat tttgctaccg aagccctgaa tgctcaccaa cgcaccctca
     1081 gaatgagagg acgccccaaa attctgctgg cccgcagcta cgaggaggcc atggaaatac
     1141 acgataaata caaggattat atgctgggca tcatctcgga tgtcagtttt atgaaagaag
     1201 gcaaaaaaac ccaaaacgcc ggtttccttt tgtgcaatga attcaggaaa tacgacaagg
     1261 cgcttcccgt catcattaat tcttcggaag cttccaaccg tacttatgca gagcaaaatg
     1321 aaatgggttt tattcaaaaa agctccaaaa cctattctaa agagctcaga cacttgctgg
     1381 cagatacctt tggtttcggc gattttgtgt ttaaaaatcc gcaaaccggc gaagaagtgg
     1441 cccgtataca aaacatacag gagttgcagt cggccatttt cgatattccc gacgactctt
     1501 tacgttatca ttttgaacgc gatcacgcct cccgctggtt gttttccagg gctatgttcc
     1561 ctatcgcccg ctttatgaaa ggtattctta tccaggatta tgaagacact gctccggtgc
     1621 ggaaaatgct ttttgacgcc attgttctct atcgcagggt aaaaaatcaa ggcatcgtgg
     1681 cagaatttca gaaaggtcgc ttcgacaagt attccaattt tgcccgcatc ggtgaaggtt
     1741 ctctgggagg caaaggcaga ggcctggctt ttctggatgt catgctcaaa agccatccgg
     1801 aactgaatac ttaccgaaat gtacatatag gcattcccaa gaccttggtt ttgtgcacgg
     1861 atattttcga tgaattcatg gataataacc atttacatga gcttgccatg tcggatcaga
     1921 cggacgaaat tattttagaa aattttctca aagcttccct gccgccccgt ctactcgaaa
     1981 acctgacagc ttttgtggat gttgtccaaa aacccatcgc catccgctct tccagtctgc
     2041 tcgaagatgc acattatcaa ccctttgccg gaatctattc cacctacatg atcccctaca
     2101 acgagcacgc agaagacatg ctgatcatgc taaagtccgc cattaaagct gtttacgctt
     2161 cagcgttttt tcaacaaagc aaggcttata tgcaagccac ttccaacctg attgatcagg
     2221 aaaaaatggc gattattctc gaagaagtct gcggtactgc gttcgatgag cggttttatc
     2281 cgaccttctc gggagttgcc cggtcgctga attattatcc cataggcgaa gaagaggccg
     2341 gcgacggaat tgccaatgtg gccctgggtc tgggtaaata cattgtcgat ggcggcacaa
     2401 ccctaagatt ctcgcccaag catcccaagc atctgttgca aatgagctcg ctgaattatg
     2461 ccctgagcga aacccagaca cattttctgg ctctggacct caaaaacaaa tccttccggc
     2521 tcaaagttga cgacgggcaa aacttgctgg agctacctat aagtgaagcc gaaaaagacg
     2581 gtaaccttaa atttctagcc tccactttcg acccactcga tcaggtaata agagacggat
     2641 tatgggaagg aggccgaaaa ataatctctt ttgctcatat tcttcaaaac gatgttttcc
     2701 cactggcaga cattttatcc aagattttga aactgggaga acaggaaatg ggacgaccca
     2761 tcgaaattga atttgcggtc gattttcacc cccgcgacga ggacgacagc ccggaacaaa
     2821 gatacttcta tcttttgcag atccgcccta tagtagatac caaagaaatg ttggacgaag
     2881 atctggacga gatccccgaa gaacaattgc tgataaaaac catttccgca ctgggccatg
     2941 gcatagtaga cgatatttac gacctgatct ttatcaaacc cgaactgttt agctcctcta
     3001 ataatcaact ttgcgtacac gaaattgaaa aaataaataa gcaactcggc gacgaaggac
     3061 gtaattacat tttaatcgga ccggggcgtt ggggatcgag cgattcctgg atgggcatcc
     3121 ccgtgcgctg gtctcaaata agcaatgccc ggctgatagt tgaaacaggt ctaagaaaat
     3181 accagatcga tcccagtcag ggcacgcact tttttcaaaa cctcacttcg tttggagtcc
     3241 attactatag cctgcccaac agcgacatag cttttaatta cgtggattac gaatacatac
     3301 acaatcatcc ggccttttac gaaaaccagt tcctgaaaca tgtaagattt gaaaatcccc
     3361 tggtatttaa aacggacggt aaaaagggga agggcgtatt gttaaaagca aatgggctta
     3421 gcgtagcttt cccaaaaatg agctaaaaag gaaagggtgt attgttaaaa agcgattagg
     3481 gacctttctg aagatcggtg aatctgctca ttaatttatt gctgaaggtc tttgaaaccg
     3541 gtatatccgg caccaggtcg ctgtccaatt cgagactgag accttcttcg cttttgcgaa
     3601 gtattttaat cttgtcaaaa ttaacgatat acgaacgatg gcaacgcacc aggtatttgt
     3661 ctccaaacaa atcgtcgatg gttttgagcg agctcctcaa cataaaatgc tgcatcttgc
     3721 ctttgtttaa ataatggatc ttgacgtaat tgtcggccga ttcaatgtaa tagaggctgt
     3781 tgatatcgac ggatagacgt aaaactccct tgtcatcgta aaaagaaatc aacccgcttt
     3841 tttgtttctc cgccagcagg ctttcctgtt cacggatacg tttcagcatt ttctcgttat
     3901 cacgccatga aaaatacaac catagaatgg cataaggcaa caacaaaatc aaactggtat
     3961 tgatggtggc atttttccaa atttcaagga aaggacgcgt atcgttgagt gcaaaatatt
     4021 gaaaaagggt ataaaacaaa gacatggcaa tgacttccat caccacccat gccagataca
     4081 aaaggacgga gatttcgtga tttttgttgt aatggtacat gatgatacgg cttatggcca
     4141 ccacacacat acccaccaaa attaccaggt tggaaaaaag aaagtaagta agatccgaaa
     4201 ctcccccctg ccaatttttt gagccgaaag gctcataaat atttataaac aatagagcaa
     4261 agacagcagt aagtaccacc aagcgcacca cattgccttt ttcgttaatg taatccggaa
     4321 tctttctgtc cataaatcag gggcttccct ctattgagaa taagtacaaa gatagaaatt
     4381 ttacatttta cccgcaagag gtaaagtaat ttcgtcccac atagaggatt ttcaccctgt
     4441 atttggacaa aaatccccca aaatccccct aagtcccttt tttttgctca agtagaaaga
     4501 cctttgtttt tttgtttcgt tcttaaaacg acaggctatg aatttagcgc aaagacaaga
     4561 ggaacttcaa agacaattta aattgggaat agataccaaa ctggagaatt caccggttga
     4621 aattcgtcat ttcccttata catctcttga cgaagataaa ggggaaaagg ccggaggatc
     4681 cgactccgat attctggaaa atcgcgaatt tgtctatccc atattcaaac taaaaagtca
     4741 ggataagtgc ccgggagcca tatttctgat gcacggactg aatgaacgca gttggaacaa
     4801 atacctgtgc tgggcagaat atctatgtat gcacacaggg aaagcagttg tcttgctgcc
     4861 cattgccttt catatcaacc ggtcaccgct gagttggctc aatccccgat tgatctctcc
     4921 tattttggaa aaacgaagac aaatcctggg caaggaacaa tctcaaagca ttgcaaatac
     4981 gattttgagt gaacgcctaa gcgaagaccc caagcgtttt taccgttccg gcaaacaaag
     5041 tctcagcgat ttgaccggcc tggtgagaca aatcaaagaa gggcgccatc ctttgtttac
     5101 ttcggggacc ggggtggatt tttttgccta ttccatcggt gcttttttag ttcaaatagc
     5161 gctactgagc gataaagagg gtttattgga agactccgga ctctttttgt tttgcggagg
     5221 ttctattttc aagcaaatga acggaacatc gcgttttatt atggacaaac tcaccttcga
     5281 taaattatgc gattattatc tctatcgctt cgaaagcgac aaccggattg accaaacttc
     5341 ggaggcctcc atgattgctt tcaacaccat gatcgagaaa gagcgcggcg ctgaagagcg
     5401 ctaccgtatc ttcaatgaaa tgggaaacaa aatccaggtg atggccttga agaaagacag
     5461 cgtaattcca tacaacggca tagtagaagc tctgggtaca gaatgcgctc aaagaaacag
     5521 cagcctgatc gacttcccct acgaatacag ccacgaacaa cccttcccca ttatgaaggg
     5581 gcaaaaagaa caaaacgacc tggtcaattt gtgtttcaat ggggtgtttt cacaggcagc
     5641 agcctttttg agtaaataaa aatccgcctg gccgggaatg ctaaccgact tatttataca
     5701 gaaaacgttt gatgctgcgt ttgggtgttt tttcaaattc cttctttacc agctctattt
     5761 tcataattcg ggcaaatgcc ggcagaagtt tattcagtga caaacggttt tgttccatga
     5821 cttcatttat ggcttcttcc ttggattctc ctccggccgc atcgaagtcg ggataaacca
     5881 aggccaccag cttttcatct ctttctacta ccaccgattc cacgaccaaa ggtaaattgt
     5941 tcaatttatc ctcgatttct tcggggtaaa tgttctgccc cgatgaacct aaaatcatgc
     6001 ttttgcttcg gcccttaatg taaatatttc cccgtttgtc cagcaaaccg aggtcgccgg
     6061 tgcgcatcca gccatcctga gtgaaagcag cttcggtggc ttcgtcgttt ttataatacc
     6121 ccaacatcac gttgtcgccc ttaacctgga tttcccctgc ttccttgtac ggattggccg
     6181 agtctatccg caattcgatc cgatgcaccg gttttccaca agaagctttt gcaaaatgtc
     6241 tccagtcttc gtaacccagc aaggggccgc attcggtcat gccgtagccc accgtatagg
     6301 ggaaacgtat ctgcttcagg caattttcaa cctcaaagtt caaagcggca cctccgataa
     6361 tcaaatacct cagtttgccg ccaaaagccg tcatcaattt ttttctgacc gcattgcgta
     6421 acacaagatt gatgccggga gtataccaca acaccttcat aacaggcttg tgtatgacgg
     6481 ggaagacggt ctttttgaag atcttttcta tcaccagcgg aacagtgagt atcatgtaag
     6541 gattggtatc tgcaaaggct ttcatcaaaa cctgaggcga aggagtttta ttcaggaaat
     6601 acacatggca accgccggcc agttgataca aaaactcaaa agtaagtccg aacatatgcg
     6661 ctaagggcaa catggagacg atattccagc ccacttggtt gggaatacgt gcctgaccaa
     6721 attccacgtt ggagcttatg cttctgtgag taagcattac gcctttgggt gcactggtgg
     6781 tgcccgaagt gtaattaatt accgccaggt catcgaaatt attcttgggg taagaaatat
     6841 cctgcatttg caaaccattg ggataatgcc ggccaaaaag ctgatctttg attgcaaaag
     6901 tctgctttac ggcagccgat ttggtttgta tgagttcaaa atttctgagc gacactatgc
     6961 tcttcaattg gggcattttt tctgcttcga ggttttccca aaccgaaggt cccacaaaca
     7021 acagcgatgc ctctgaatga ttcaccagtg catgaatgct gtccacatga aaatccggca
     7081 aaatagacac caccacggct tcgtaggtat tcgccgccaa aaaggacatt gcccagttaa
     7141 cactgtttct gccacagatg gcaattttct ctcccttttg aagccccatt tccttaaaca
     7201 acaaatgcaa ctgggcgata tgttcagcca gttcgccata ggtcatggtg aaatcggttt
     7261 catagtcggt tatggccgga gcgtcccaat ggtttttgat ggtgtcttcc aggaagctaa
     7321 aaaaatgttt gctcattgcg gtaaattaat tttgtttttt gaagttaggt tcactatcag
     7381 ggttaaccgg ttcaaggttt tccctttgaa attttttgcc gggcaaaagt agcctcgtgc
     7441 gcgggcattt aaaaaaaaat gggctatgcc atctcaaaac tgggacgaaa acacaaatgt
     7501 gtacgccaaa tgccctattt agggacgaaa gttctgcgac tgcaggcttg aaaaagcctt
     7561 ccctaaatga aattatcaaa gcatattcca attatttgct atatttgtgc ctctcaattt
     7621 gaatcatatt ttaataatct aactattacc cactatgcaa cttaaaggtt ccaaaacaga
     7681 acaaaacctg cttaaagctt ttgcaggaga atctcaagct aaaactcgtt atgaattttt
     7741 tgccagcgta gcaaaaaaag aaggttacga acaaatcagc gctttcttta ccgaaactgc
     7801 cctcaacgaa aaagaacatg ccgaattgtt ctttaaatat ctcgagggag gaatggttga
     7861 aatcacagcc tcttatcccg ccggtgtaat cggcactact gccgaaaacc tgaaagctgc
     7921 cgccgatggc gaacacgaag aatgggccga gttatacccc ctgtttgcac agatagccaa
     7981 agaggaaggt ttcaaagaaa tttccgccac ttttactctg gtagccaaag tggaagccga
     8041 acacgaaaaa cgctatcgca agttgttgca aaacatcgtc gaaaacaaag tgttcgaaga
     8101 cgacgaaaac accacctgga cctgtcgcaa ctgcggctac gtgcacaagg gcaaaaacgt
     8161 tcccgatctc tgccccgttt gtaaacacgc taaagcctat ttcgaaagaa ggtgcgaaaa
     8221 ttattaaaag cgctgaattt ggtataaatc agtctgtggc tattcttact tgaatcgcca
     8281 ccagtcgaaa ttaaacaaat tcttccccgt acccctgaag acaaaataga ggtcatgggt
     8341 tccgcgcaat ctgggtttca atttacaact taccgtgtga tattgtgatt caccgccggt
     8401 aggtgatatg gatagtgtgc ccgctaacgg accatccgga ctgtcgagac gtatttcaat
     8461 gcgggccagg ctgtcggtgg caaccgaagc atagaacttc ttggcgcctt tatttccgaa
     8521 ttttacaccg ctaaccttta tgtgctcacc atcatcaata ttggtgacaa acatattggt
     8581 agagttttta ttctcctcgt ccatgcgctt agttttcaag ccatagcccc aggacatggt
     8641 ctcagcctct acacgacgat agggatcaaa cggtttgatc tgctcaacca cattatccat
     8701 aaaataggga acttcctgaa tggtgccatc gggattatag tgcatctcgg ccaatgctgc
     8761 cgaacgctgc tcggcatggg caaatgttgt aaggtgcata atatcataat tcagaccaaa
     8821 cacataatgc ttgcccttat acgagattat tccggggtgg ttgccgcggg tgcgtaccgt
     8881 atggtccatt atatgccctt tgtactccca gggtccttcc ggaccgtcac tcatagcata
     8941 accgatgcct tccggacagc atgtcgatgc aaaagccaga tagtannnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnncggac ataagtacgc ctattccgcg accatgtatg
     9121 gggcagtaca tatagtatgc tccgttggcc tcgaccacct cctgggccca cgcgccgttc
     9181 tttccgtcat agtacttaaa gtcatcgagc gaagccacag cgccatggtc ctgccagttt
     9241 accatatcgt ccgatgtgta aagcagccag tcaaacatct ggaatccgcg ggcgccgtct
     9301 tcatcgtgtg tggtataaag gaataccgtg tcgttgatca ccacaggggc gggatcggca
     9361 gtgaagctgg tctgtattat gggcatattg gcatgagagt tgaactgcca ccagtcgaaa
     9421 atgaaaggct cgggagtctc gcatcggaac acaatataca ggtcatgtac accggtgacg
     9481 tttgtatcta tcaggtacga aaaagaagca gtgggaaatc ccgcattgtc cgtatttacc
     9541 ggaacagtgc agaacggctc gccttccatg ttgtctatat ggaattcaat ggttcctttg
     9601 cctctctgcc ttcctacaac cacggtgaca agtttttgac cgcaatcctt aaaatcaacc
     9661 gagcgcacgc gcatccagtc gccatcgtgt atatcagtca catagtgatc tctgccggca
     9721 agacggtcat tcttcagccc gcgtgcctct gccatcgttt cggcctcaac acgtttatat
     9781 gggtcaagat tcttgatagg tgttaccaca ccctcttttg taaaagggat gaacggaatg
     9841 gagccatcct cattgaactt gaactcctct acacaggcgg agcggttaaa gccgccgcct
     9901 cccggaagca tgccgttatg ataaaacatg aagtcacgcc ccttgaacgt tatatttcct
     9961 ccgtgaatgg taaaactgtt ttctgcctca tccatgatac ggccccgata ggtccaggga
    10021 ccatgcacgg taggcgctgt cgagtaagcc atatgttcag gtacgccgcc tgccggataa
    10081 gagaggtagt agataccgtt ccgcttcata agccacggcc cctcctcaaa ctgggtcatc
    10141 atttcattac ggccgcgcgc ccaattcatc ttggattgca gctctccgaa agatttcgga
    10201 tcacgatagc cgggcacctc tttccagcca tccttaaacg agaccatatc atcgttcagt
    10261 tcaccgtacc aaagaccttt attgccccag aaaagatagg cacggccgtc atcgtcaatg
    10321 aataccgtag ggtcaataaa gccgaatccc tcggccagag gcttgcctat ggcatctttc
    10381 catgggcctt gaggattgtc tgcaacagct acagccaggg catcgccgcc gcctgcaatg
    10441 ttgcagcaga cataccagta ccacttgccg tcacgctcca caccttgtga ggcccaagcc
    10501 atcttgccct gtcttgccca tgagaaagtg gcggttgtga cctgtgtgcc gagataggtc
    10561 cagttcacca tatcctcggt gctgaacaac aaccagtctt tcatgttgaa gtccgtattg
    10621 cgtccgtcct catcatggtc ggtaaacaga tagaccttgt caccatatac gaaaggtgca
    10681 gggtcgggtg taaacatcgt gcttattatc ggattttggg caaaggccgg taccgtcaaa
    10741 gcgactgtaa aagtcaataa tagttttaag gttctcatag gtttatatca ttcagtttag
    10801 taattcttta atcagtcaag attacaaatc cgccgcgagg ctgcaaaata aaactttcgg
    10861 gcaggtcgac attctcattg atctcgaaac cctgaccggg ttcctgaccc ctgtcggtaa
    10921 ataccgtcac cttaccttgc tgctccagcc tgctgatatc gggagtgagt tcaagaacct
    10981 cgtcggtacc gttgatcccg gctatgtacc accggtctct gctgcgacgg gcaataaccg
    11041 cgctctcacc gggatatccc gcaagcaggc gggtgtcgtc ccataccgaa ggcagcccca
    11101 taagcagctc tttgacttgg taaggcagcg acagatagga ttccggacga tcagccatat
    11161 gttgcagacc cgactcaaag agtatgggaa gcgccaactc gtgacaatcg gtagtaatat
    11221 gcttgtgctg actatccgaa aaggtgcagg gagtataatc catagggcct atgacgttgc
    11281 gcgtaaaagg cagcgtggca ttgtgtgaag ctgcggcttg tgtcatgcgg ggcgtgttgt
    11341 tataccactc ggcaccataa acagcctcca tagtcataag attaggccag gtacggttcc
    11401 atccgcgcgg aacagtacag ccgtggaaat ttaccagcag ccggtgtcgg gcggcatcct
    11461 caagtatatc aaggtagtag tctatcatct ctgcaccatc gggcaagaaa aagtccacct
    11521 tgatgccgct tactcccata ttgttcagac gtgtcatctc actctcgcgg gcctcggccg
    11581 tacgcagacg atccagggga gtcggagcgc ccggacctac ccaggaggtg ccggagttgt
    11641 accataaatt cacctttata cccttttccc gggcgtatgc catggcatcc tcaatgtcgc
    11701 ctcctcccgc catctcgtcc cactcccaat ctatcagggt gtagggccaa ctcatctcgg
    11761 cggccaggtc aatatattct tttaccttac cgaaatcttt cgacgagcgg ttgtatgccc
    11821 aatatatcca cgatgagaga cccggatgta tccaggagat gtcctctatt tggcaggggg
    11881 tggccacatc ggtaaccaga gttgattcca caatatcggc aagcgaacct atgataagca
    11941 caaaagccgg agagataccg ttttcggact taccttcgga catgtattta atatggtaag
    12001 cgttatcatc ccgcacttcc agatgggagc cgctcatacc gtgctgcacg tccgattcag
    12061 aaatcagccc gaacaggcca tcggcatact cggtaagcag cggatagccg taactcccgc
    12121 tgcgtgcctc cgtccgcatg ggatagaaac cctcatagtc ggtcctcagg tt
//
LOCUS       DAOSTD010000053        12169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_5230_length_12169_cov_14.030296, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000053 DAOSTD010000000
VERSION     DAOSTD010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12169
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5230_length_12169_cov_14.030296"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1075
                     /gene="metF"
                     /locus_tag="PLI37_04870"
     CDS             188..1075
                     /gene="metF"
                     /locus_tag="PLI37_04870"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOQ58376.1"
                     /translation="MKIPDILRNKKTLSFEIFPPKRQEDTFVLIENTIKELSDLGPDF
                     ISVTYGALGNTLSNSVAIAKHIKQHTRSEALAHLTCVSSDKAQVDEICRELREANIKN
                     ILALRGDLPADPGMHHHDFRHASDLTAYISQRYGADFAFAGACYPEKHPEAISMKEDI
                     RHLKIKQDAGMDFFITQLFFDNEAFYRFLLEARKAGITIPIIPGIMPVSNYKQLDGMI
                     RVTGAAIPLQLRNYFDRYRYDKQALEEIGIIFTSHQILDLIANDVDGIHLYSMNKSSF
                     IKKLMANIAYVRSGFFAQE"
     gene            1044..1715
                     /locus_tag="PLI37_04875"
     CDS             1044..1715
                     /locus_tag="PLI37_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOQ58377.1"
                     /translation="MCVPVSLLRSNALTYLGYSDSDLDEQTQEALHSALTHIAEWSAY
                     KQIYTRLKDLPEAPAFLQQESYVGLQGKLDTALLFAATLGLGIDRRIQSLSLNRLSEA
                     VVLNAVANAYLETRVELFIGEILPGGVLFCPGYAGTSPEGNRSVLKLLNAQKHLGIYL
                     KDSGLMIPEKSMAGIVVAGYSFSCRACSIRSKCDHLRQGKTCYGKPAPHREESRNAKA
                     DNIKA"
     gene            1756..>2377
                     /locus_tag="PLI37_04880"
     CDS             1756..>2377
                     /locus_tag="PLI37_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOQ58378.1"
                     /translation="MPDLISKKVQLFDGAMGTILTAYPEVNSFLPEELNLLFPEKILA
                     IHRAYVEAGAQYITTNSFSANPIKLNSSRFSLQEVLDAAIELARWAAGESSCKVMLNI
                     GPTGKLLEPLGELGFEETYENYKTVVEYTKDKVDGYVLETFSDLYEIRAAILAVKENS
                     TLPLMATMTFDTSGRTLTGSSPEIVALTLSALGVDVPGINCSTSPEH"
     assembly_gap    2378..2477
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2478..>3984
                     /locus_tag="PLI37_04885"
     CDS             <2478..>3984
                     /locus_tag="PLI37_04885"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOQ58379.1"
                     /translation="SALGVDVPGINCSTSPEHLIPLVKRMRAFTHLPLLVQPNRGFPI
                     LCRGSVSYAMSDEDFVYYTGKLIDAGASIVGGCCGTTPATIALMAKYKSLPLKEYAPV
                     AGHYICSSTRLLKLQQGLVCGERLNPTGKKKLQQALLNSDFGYLQQEALSQQEAGAHF
                     LDLNVGIPNADEVDLLCRATKKIQEVCDLPLQLDSSNPAAIGAALRVYNGVPVINSIN
                     GSGPVLKALLPLLAKYGASAVALTLDEQGIPETVEGRLRIAKRIIARAAAKGINKNRL
                     IFDALVMAVSSNQHYGQLTLDTLRSLKKMGVLTTIGLSNVSFGLPNRPLLNRSFLLMA
                     LTSGLDIPILNPLDKENMQSLKAFNALSGADPACENYISYHSPSPVHEQSDSLYQVVL
                     SGLKDSLPLAMDKILKEKEPQQIIDEDLIPALNEVGQRFEKKHVFLPQLIRSAEVARQ
                     AFQQLGELYVQKTDKTQGLVVLATVKGDIHDIGKNIVKVVLESHGYRVLDLG"
     assembly_gap    3985..4084
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4085..4501
                     /locus_tag="PLI37_04890"
     CDS             <4085..4501
                     /locus_tag="PLI37_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ58380.1"
                     /translation="HIGFVFQFHQLLPEFTALENVSIPALIAGKSKKEAEALAMEMLD
                     HLNLSDRINHKPSELSGGENQRVAVARALVNRPKVVFGDEPSGSLDSKNKAELHRLIF
                     DLRERFGQTFIIVTHDKELARLTDRVIEIKDGVILS"
     gene            4513..5958
                     /locus_tag="PLI37_04895"
     CDS             4513..5958
                     /locus_tag="PLI37_04895"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOQ58381.1"
                     /translation="MKKILLVALIPVLLSMLSCRHKQTKDARTENQTSKITVLIEYAD
                     STVKVSEQAFGIRVTRVGMHVVAVSRRKNVEFILRGKAAEGSFKLYSDYSFALRLDGV
                     ELFNPEGSAINIQSPQHALILVSPGSENLLRDGSLYAIPPEGEQMNACLYSEGNLIFY
                     GSGSLLLKGNYKHAIYCRGYLKVNDNCDIRISEAKKDGIHTLGDVFIEGGQLNINVAD
                     DGLKSASNILINKGNIVIRALKKDGSEGLECNRLTVNGGQMSVLAYDDAINTARELII
                     NGGSVFCYSLYNDGIDSNGSIQINGGLVVSSGSQMPEEGIDCDRNAFVITGGTVVACG
                     GGTSVPTVRLSTQPSLIYSGDFEQSIHICGPGCEDVLTYFPQRNYHRFTLLFSSPALK
                     LGSDYRLYSGGQLSGGTQQHGYYTGSTYRNGVKKADFTASTMVTMIGEFHMGGPIHRG
                     AFPINPPEEPPFDPADFPQYPPAENQAPGEL"
     gene            complement(5965..8565)
                     /locus_tag="PLI37_04900"
     CDS             complement(5965..8565)
                     /locus_tag="PLI37_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOQ58382.1"
                     /translation="MKSKLPALLLGLLLSCPLYCVFANDLSLWYNKPAANWNEALPLG
                     NGRLGAMVYGDALNETIKLNDNTLYSGEPATVWKVHDITPTYDKVVGLLLEGKYAEAN
                     ELVQYHWLGRLHENYQPLADWHLTNHTQGEISDYRRELDIANSVFRMSYKQNGLNYTR
                     EMFISHPDQVIVMKMTCDKQAEFDFSLHFTSHHRATTVFGIEENVYKVSGQAPGHGQR
                     RGDAQIRGWKAEGRHPELYFADGSRKYQENVLYGDQINGLGTFYEARLQVLAPGSKIV
                     SEDKVMRVTGGNEIVFILSAATSFNGFDKSPSRQGKDAVKLAEEALSKAAQKSYENLK
                     KDHVRDYKQLFDRVSFEMPYKKELASLPTDERIDRFYDDYDNGMITLLYQYGRYLMIS
                     ASRPGGQPMNLQGIWNDKVMPSWNCGYTLNINAEMNYWPAEPGNLPECHEPFFRMIKE
                     MAVSGNKTAREMYGRNGWVAHHNTSIWRETHPNDGTPTSSYWPMASAWLSSHLWEHYL
                     YSGDKEFLQNEAYPIVKGAAEFFTDWLVDNGEGYLVTPVSNSPEHNFIYGEYQKAAIS
                     MGSTMDMALIRELFTRTIEASETLGVDEDFRNKLSLQLEKLLPYRIGARGQLQEWQQD
                     FRDGEPRHRHVSHLYGLHPGNQINLDETPELFQASMRTLELRGDEATGWSMGWKINLW
                     ARLLDGDHANIIIRNLFNPIGFGKSKLSGGGLYANMLDAHPPFQIDGNFGYTAGIMEM
                     IVQSHAGFVHLLPAIPREWSSGKISGIKTRGGYELDIEWENGKISRAVLHSSLGGNCR
                     LRSSEPLRIKGAKVKTAEGQNPNPLFKTVSAGGFQDYSQVGATKLPDKKYYTVDFMTE
                     KGKSYVITPQ"
     gene            8812..8973
                     /locus_tag="PLI37_04905"
     CDS             8812..8973
                     /locus_tag="PLI37_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOQ58383.1"
                     /translation="MKKYRCTVCNYIYDPAEGDPDSDIAPGTAFEDLPEDWTCPLCGV
                     GKEDFEPED"
     gene            9009..9662
                     /locus_tag="PLI37_04910"
     CDS             9009..9662
                     /locus_tag="PLI37_04910"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOQ58384.1"
                     /translation="MNSKFVILLTCIIMSIQSAGAKAPFEKPQLKYAHNALEPSISAE
                     TIEYHYGKHLQAYIDNLNKLAAGTAFAEMSLDDIVLKAEGGILNNGAQSWNHIFYFDS
                     FSPKAKTAPGGKLLEAINRDFGSLEAFKEQFNKAAATLFGSGWAWLSVDKNGKLVITA
                     ESNAGNPMRSGLKPLLTIDVWEHAYYIDHRNRRPDAIAAFWKVLDWSVVEARFENKL"
     gene            9721..10545
                     /locus_tag="PLI37_04915"
     CDS             9721..10545
                     /locus_tag="PLI37_04915"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOQ58385.1"
                     /translation="MKPPYTITPLILRSVSSISEKLGEVNASRFRWPSSAWRKRNTLL
                     KVHASLRLEGNKMSLRQVKAVQEQRQVKADPRDILEAQNALRVYDELSLMDPFSSKDF
                     LKTHKMLMNGLVELPGKFRKREIGIVHGDNVAHIAPPAKKLTYLMQDLFRYLKDSEEP
                     MLIKSCVFFYETEFIHPFLDGNGRMGRLWCSLLLSRINSLFMYIPLEHYLLESRDEYY
                     KALAISDSKGASTAFVEYLLQKIEQAFNDFLIERKVLSKNSHIYEENKKNAEPKKA"
     gene            10505..11236
                     /locus_tag="PLI37_04920"
     CDS             10505..11236
                     /locus_tag="PLI37_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase"
                     /protein_id="HOQ58386.1"
                     /translation="MRKTKKTQNQKKREYFEILNTISKGRHRTNILKKPEQSALAYLV
                     KRIPAWVSSDMLTFFGFCGSLLTAASFVMAAYWSRLYLLLGILGFIINWFGDSLDGRL
                     AYYRNKPRKWYGFSLDITVDWLTTVLIGSGYSLYTEGFAKFFGLAFVVMYSWAMITTL
                     MRYKVTDQYSIDTGILGPTEVRVVISLILLAEVFIKDSIVYTGALACVVLLVVNVIST
                     VELLIVADKRDKDEKESNAGSKTEG"
     gene            11266..12129
                     /locus_tag="PLI37_04925"
     CDS             11266..12129
                     /locus_tag="PLI37_04925"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOQ58387.1"
                     /translation="MKTHVVDISDFEQFSPVFRGKLGNLLAGQIIKLFALDKVNALYQ
                     RCCDHTGAAFASALLRDLGVQYRIGNPDRLKQLPEGAFITVSNHPYGGLDGIMLIDLM
                     AHIRRDYKLMVNEILSLIEAMDVNFISVRPQGNKKLPPTTNLDGVRETISHLRKGHPM
                     GFFPSGAVSDFSFRNRRVQDRPWQDSVIKLIKKAKVPILPIRFFDGNSPFFYFLGTIN
                     WRIRLIRMPREVFNKSTQFPRLGIGKLITVEQQSRHRNTEDFGALLRSSVYDMPVPAQ
                     FTPRERVFLDG"
BASE COUNT         3043 a         2814 c         2927 g         3185 t
ORIGIN      
        1 tggcggctta tacgattaag tacctgattt ctgaaaattc ttccacgaat aatcagccgg
       61 attgaaccgg cccggccgta tctaaaaagt tcagattgtt gataaatagg cacaaaatgt
      121 attttccctt ttgcacttat acgggaatat tgtctatttt tgcgctttgt tttaattgct
      181 ctgatctatg aaaatacccg atatacttcg gaacaaaaaa accctttctt tcgagatatt
      241 tccacccaaa agacaggaag atacttttgt acttatcgaa aataccataa aggaattatc
      301 cgacttgggc cccgatttta taagcgtcac ctacggggcg ctgggaaata ccctcagtaa
      361 ttcggttgcc atcgccaaac acattaaaca acacacccgt tcggaagcct tggctcacct
      421 cacctgtgtg tcgtccgata aggcccaggt ggacgaaatt tgccgggaac tcagggaagc
      481 caacattaaa aacattctgg ccctccgggg cgatttgcct gcagatccgg gcatgcatca
      541 tcacgatttt cggcatgcct ccgatctgac cgcttatatt tcgcaacgtt acggagccga
      601 ttttgctttt gccggagcct gttatccgga gaaacatccc gaagcaattt ccatgaaaga
      661 agatatccgg cacctcaaaa taaaacagga cgcagggatg gactttttta ttacccagtt
      721 gttttttgac aacgaggctt tttaccgttt tttgctcgaa gcccgaaagg ccggtatcac
      781 catcccgatt atcccgggta ttatgcctgt gtccaattac aaacagctcg atggcatgat
      841 acgggttacc ggagccgcca tacccttgca actgcgtaat tactttgacc gttaccgtta
      901 cgacaaacaa gccctggaag aaatcggcat tatttttacc agccatcaga tcctcgacct
      961 tattgcaaac gatgtggacg gtattcatct gtactcgatg aacaagagca gttttatcaa
     1021 aaaattaatg gccaacattg cctatgtgcg ttccggtttc tttgctcagg agtaatgccc
     1081 tgacttatct gggttattcc gatagtgatt tggatgagca aacgcaggag gcccttcatt
     1141 cggccctaac tcatatagct gagtggtctg cgtacaaaca aatttacact cgcctgaaag
     1201 atttgcccga agcaccggca tttttgcagc aggaatccta cgtgggcttg cagggaaagc
     1261 tggatacggc tttgttgttt gccgccaccc tcggtctagg catcgatcgg cgtatacaga
     1321 gcctttcgtt aaacaggctt tcggaagctg tggtgttgaa tgccgtcgcc aatgcctatc
     1381 tcgaaacaag agttgagttg tttatcgggg agatattgcc cggaggagtc ttgttttgtc
     1441 cgggttatgc cggtacctcg cccgaaggca atcgctcagt gttgaagctg ctcaatgccc
     1501 aaaagcacct gggtatttac ctgaaagatt ccggactgat gattcccgaa aaatctatgg
     1561 ccgggattgt tgttgccggt tatagtttca gttgtagagc ttgcagcata cgcagcaaat
     1621 gtgaccatct taggcagggt aaaacctgtt acggcaagcc ggctcctcac cgggaagaga
     1681 gccggaatgc aaaagcagac aacatcaagg cataaaaaga taaagtaaag aacagcaaaa
     1741 agaagaatca atacaatgcc cgatctcatt tcaaaaaaag ttcagctatt cgacggcgcc
     1801 atgggtacca tactgacagc ctatcccgaa gttaattcct ttttgcccga agagctcaat
     1861 ctcttattcc ccgaaaagat tttggccata cacagggctt atgtagaggc gggagctcag
     1921 tatattacaa ccaattcttt cagcgccaat ccgatcaaat taaactcaag tcgttttagt
     1981 ttacaagaag tgctcgatgc tgccatagag cttgcccgct gggctgccgg agaaagctcc
     2041 tgcaaagtca tgctcaacat aggtcctacg ggaaagttgc tagagccctt gggagagctc
     2101 ggttttgagg agacctacga aaattacaaa acagtagtgg agtacaccaa agacaaggta
     2161 gacggttacg tgttagaaac tttttcggat ctctacgaaa tcagggcagc catactggcc
     2221 gtaaaggaaa attccacact gcctcttatg gctactatga ctttcgacac ttcgggcaga
     2281 accctgaccg gttccagccc tgaaattgta gctcttacgt tgagcgcctt aggcgtagac
     2341 gtgcccggca tcaattgttc cacttcgccc gaacaccnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnagc gccttaggcg tagacgtgcc cggcatcaat tgttccactt
     2521 cgcccgaaca cctgattcct ttggtcaaac gcatgagggc ctttacccat ttacctttgc
     2581 tggttcagcc caacaggggt tttcccattc tttgccgggg atcggtatcc tacgccatga
     2641 gcgacgaaga tttcgtttat tatacgggaa aactcatcga tgccggcgcc tctatcgtgg
     2701 gaggctgttg cggcacaacc cccgcaacca tagccctgat ggctaagtat aaaagcttgc
     2761 cgctcaaaga atatgcccct gtggccggcc attacatttg ttcttcgacc cgcttgctta
     2821 aattgcaaca aggcttggtt tgcggagaac ggctcaatcc caccggtaag aaaaaattac
     2881 agcaagccct tttgaacagc gatttcggct atctgcagca ggaagccctc agccagcagg
     2941 aagcgggcgc tcactttctc gatttgaatg tgggtattcc caatgcggac gaagtcgatt
     3001 tgttgtgccg cgcaaccaag aagatacagg aagtgtgtga tttaccctta cagctcgaca
     3061 gttccaatcc tgcagccatc ggggcagctc ttcgggtcta caacggagtg ccggtaatca
     3121 attccatcaa cggcagcggg ccggtgctta aggccttgct tcctctgctg gccaaatacg
     3181 gtgcttcggc cgttgccctc accctcgacg agcagggcat acccgaaaca gtggaaggac
     3241 gtctgcgtat tgccaaacgc atcattgccc gggccgcagc caaaggcata aacaaaaacc
     3301 gtctcatctt cgatgctctg gtaatggcgg tttcgtccaa tcaacattat ggtcagctta
     3361 ccttagacac cctgcgcagc cttaaaaaaa tgggagttct taccaccata ggcttgtcca
     3421 acgtgtcctt tggtttaccc aaccgtcctt tgctgaatcg cagttttttg ctgatggctt
     3481 tgacttcggg tctcgatatt cccattttga atcctttgga caaggagaac atgcaaagcc
     3541 tcaaggcttt caatgcctta tcgggggcag accctgcttg tgaaaactat atttcctatc
     3601 attccccaag tccggttcat gaacaaagcg acagccttta ccaggtggtg ctcagtggtt
     3661 taaaagactc attgcctttg gccatggata aaatcctcaa agaaaaagag ccgcaacaga
     3721 tcatcgacga ggatttgatt ccggccttga acgaggttgg tcagcgtttc gaaaaaaaac
     3781 acgtattttt gcctcagctc atccgttcgg cagaagtggc ccgccaggct tttcagcaat
     3841 tgggggaatt gtatgttcaa aagacagaca aaactcaggg attggtggtg ttggctacgg
     3901 ttaagggcga cattcacgat atcggcaaga acatcgtcaa ggtggtgttg gagagccatg
     3961 gttaccgggt gttggactta ggcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnncacata ggttttgtgt ttcagtttca tcaactttta ccggaattta ccgctttgga
     4141 aaatgtgagc attccggctc tgattgcggg caaaagcaaa aaagaggccg aagccctggc
     4201 catggaaatg ctcgatcacc tgaatttgtc cgatcgtatt aaccacaaac cttcggaact
     4261 gtctggggga gagaatcaac gggtggcggt ggccagagcc ttagtaaaca ggccaaaagt
     4321 ggtttttggg gacgagcctt cgggtagtct cgacagcaaa aacaaagccg aactgcaccg
     4381 gttgatcttc gacttacgcg aacgtttcgg acagaccttt atcattgtaa cccacgacaa
     4441 agaattggcc cgcttgactg accgggttat cgaaataaaa gacggcgtca tcctatctta
     4501 atcgaaagaa tcatgaaaaa aatcctcctt gtcgcattaa tccctgtttt actaagcatg
     4561 ttgtcctgcc ggcataagca aaccaaggat gcccggaccg agaatcaaac atccaaaatc
     4621 actgtgttga ttgaatatgc agacagtacg gtaaaagttt cagagcaggc gttcggcatt
     4681 cgtgtcacca gggtgggtat gcatgtggta gccgtctccc gcagaaaaaa cgtggaattc
     4741 attctgaggg gtaaagccgc cgaaggctca ttcaaactct acagcgatta ttcttttgcc
     4801 ttgcgtcttg acggggtgga attgttcaat cccgaggggt cggccatcaa tatccaatct
     4861 ccccaacatg ctttgatttt ggtgtcgccc ggaagtgaaa acctgctcag agacggttcc
     4921 ctgtacgcca ttccgcctga aggagaacaa atgaacgctt gtttgtacag cgaaggaaac
     4981 ttgatttttt acggttcggg aagtttgctc ttaaaaggga attacaaaca cgcgatttat
     5041 tgcaggggtt atcttaaagt caacgataat tgcgatatca ggataagtga ggcaaaaaaa
     5101 gacggtattc acaccttggg cgacgtgttt atagagggcg gtcagctcaa catcaatgta
     5161 gcagacgacg gcctgaagtc cgcgagcaat atcctgataa acaagggaaa tatcgtcatc
     5221 agagctttaa aaaaagacgg ctccgaaggt ctggaatgca atcggctaac ggtaaacggc
     5281 ggccaaatga gtgtgctggc ttacgacgat gctatcaata cggccaggga attgattatc
     5341 aacggcggtt ctgtgttttg ctacagttta tacaacgatg ggatagacag caacggttcc
     5401 attcagatca acggaggcct ggtggtctct tcgggtagcc aaatgccgga agaaggaata
     5461 gattgcgacc ggaacgcttt tgtgattacc ggaggtactg tcgttgcctg cggaggaggt
     5521 accagcgtgc caacggttcg tctgtccaca cagccttcgc tgatttattc cggagatttc
     5581 gagcagagta ttcatatctg tgggcccggc tgcgaagatg ttctgaccta tttcccgcag
     5641 agaaattatc accggtttac ccttttattc agcagtccgg cactcaaact gggttccgat
     5701 taccgcctat attccggcgg acagctttcg ggaggaactc aacagcatgg ctattacacg
     5761 ggttcgacct acaggaatgg agtaaagaaa gccgatttta ccgcttcgac catggtaacg
     5821 atgatagggg aattccatat gggaggcccc atacacaggg gagcctttcc tatcaacccg
     5881 cccgaagaac ctcctttcga tcctgcagac tttcctcaat atcctccggc agagaaccag
     5941 gctcccggcg agttgtaaag cgggttattg gggtgtgatt acgtagctct tgcccttttc
     6001 ggtcataaaa tctaccgtat agtatttttt atcgggcaac ttggtagctc ctacctggct
     6061 gtagtcttga aaacctccgg cgctcaccgt tttaaacaag ggattcgggt tttgtccttc
     6121 cgctgttttt actttcgctc ctttaatcct gagcggttcg gagctgcgca ggcggcaatt
     6181 gcctccaaga cttgaatgga gtacggcccg gctgatcttg ccgttttccc actcaatatc
     6241 cagttcgtat cctcctcggg ttttgattcc gctgattttt cccgaagacc attcccgggg
     6301 aatagccgga agcagatgta caaaaccggc atgactttgc actatcattt ccataatacc
     6361 ggccgtgtaa ccaaagttac cgtcgatctg aaagggtgga tgagcatcga gcatattggc
     6421 atagaggccg cctccgctca gttttgattt gccgaaaccg atgggattga ataggttgcg
     6481 aattattata ttggcatgat cgccgtcgag caggcgagcc cacagattga ttttccagcc
     6541 catagaccag ccggtggctt cgtcgcctcg gagctcgagc gtacgcatgc tggcctggaa
     6601 caattcgggc gtttcgtcca gattgatttg attgccggga tgtaggccgt agagatgtga
     6661 gacgtgcctg tggcggggtt cgccgtctcg gaaatcctgt tgccattcct gcaactggcc
     6721 ccgggcgccg atgcggtagg gcagtaattt ttccagttgc agactcagtt tgttgcggaa
     6781 atcctcgtca accccaagag tctcggaggc ttcgatggtt cgggtgaaca attccctgat
     6841 cagggccata tccatggtgc ttcccatgct aatagccgct ttttggtatt cgccatagat
     6901 gaaattatgt tcgggagaat tgctcacggg agttaccaga tagccctcgc cattgtccac
     6961 cagccagtcg gtaaaaaact cagccgctcc tttgacgatg ggataggcct cgttttggag
     7021 gaattccttg tcgccgctat acagataatg ttcccaaaga tggctgctca gccaggcgct
     7081 ggccataggc cagtaggagg aggtgggcgt accgtcgttg ggatgggttt ccctccagat
     7141 ggaagtgttg tggtgggcta cccagccgtt gcggccgtac atttcgcgtg cggttttgtt
     7201 gcccgagacg gccatctctt ttatcatccg gaaaaagggt tcatgacatt ccggcaaatt
     7261 tccgggctct gccggccagt aattcatttc ggcattgatg ttgagagtat agccacagtt
     7321 ccacgagggc atgactttgt cgttccagat gccttgcagg ttcataggct ggccgccggg
     7381 gcgcgaggcg ctgatcatca ggtagcgccc gtattggtaa agcaaagtga tcataccgtt
     7441 atcgtagtca tcgtaaaacc ggtcgatacg ttcatcggtg ggcaggcttg ccagttcttt
     7501 tttgtaaggc atttcaaaac ttacccggtc gaacaattgt ttataatccc gaacatgatc
     7561 ttttttcagg ttttcgtagc ttttttgagc ggctttgctc aaggcctctt cggccagttt
     7621 gacggcatcc ttgccctggc gtgaagggct tttgtcgaaa ccgttaaagc tggtggcagc
     7681 cgataatatg aatacgattt cgtttccacc ggtcacccgc ataaccttgt cttcggatac
     7741 aattttggaa ccgggagcca ggacttgcag gcgagcttcg taaaaagtgc ccaggccgtt
     7801 gatctgatct ccgtagagaa cgttttcctg gtacttgcgg ctgccgtctg cgaaatagag
     7861 ctccggatgc ctgccttcgg ctttccagcc cctgatttgg gcatctccgc gtctttgacc
     7921 gtgtccgggg gcttgtccgc ttactttgta tacattttct tcaataccga aaacagtcgt
     7981 cgccctgtga tgcgatgtaa aatgcagact gaaatcaaat tcagcttgct tgtcgcaggt
     8041 cattttcatc acgatcacct ggtcgggatg tgaaatgaac atttcgcggg tgtaattcag
     8101 gccgttttgc ttgtagctca tgcgaaaaac cgagttggca atgtcgagtt cccgcctgta
     8161 atcgctgatt tctccctggg tatggttggt taaatgccag tctgccaagg gttggtaatt
     8221 ttcgtgcaaa cgtcccagcc aatgatattg cacaagttca ttggcctcgg cgtacttgcc
     8281 ttcgagcagc aaaccaacga ctttatcgta agtaggtgta atatcgtgta ctttccatac
     8341 cgtggcgggt tcaccgctgt agagggtatt atcgttgagt ttgatggttt cgttcagggc
     8401 atcgccgtaa accatggcgc caagccgtcc gttacccaga ggcagggcct cgttccagtt
     8461 ggcggccggc ttattgtacc agaggcttaa atcgttggca aatacgcagt aaagcgggca
     8521 gcttaacagc agtcccagaa gtaaagcggg caatttagat ttcatagtcg gtggttaagg
     8581 taatggagaa agacatacat tgccgcaaac atacaactat tctttttccc gttgggttaa
     8641 aagctgccaa taaataatta aaatcagaaa atccactttg agcatttaat tttaaaatcc
     8701 acgattaata ggtttgggcg gtcgcttatt ttttctatct tgcgcagaat ttgttcggac
     8761 catacatgag aaagtccgtt ttttgtaaac aattttttaa accgataaat tatgaagaag
     8821 tatcgttgca ctgtttgcaa ttacatttac gatccggccg aaggggatcc ggatagtgac
     8881 attgctccgg gaacagcttt tgaagatctt cccgaggatt ggacctgtcc tttgtgcgga
     8941 gttggaaaag aggattttga gcctgaggat taggatagga ttttacacca tccccaaatc
     9001 tacttattat gaattctaaa tttgttatac tattaacctg tattattatg agtattcaaa
     9061 gtgcaggagc taaagctcct tttgaaaagc cccagcttaa gtatgcccac aatgccctgg
     9121 aaccttcgat tagtgctgag accattgaat atcactatgg aaagcacttg caggcttata
     9181 ttgataacct aaacaaatta gctgccggga ctgcatttgc cgagatgtcg ctggacgaca
     9241 ttgtgcttaa agccgaaggc ggcattctga acaacggagc ccagagctgg aatcatattt
     9301 tctatttcga cagcttttca cccaaagcca aaactgctcc gggcggaaag cttctcgaag
     9361 ccatcaaccg tgatttcggt tcgctggagg cgttcaaaga acaattcaat aaagcggccg
     9421 ccactttgtt tggatcgggc tgggcttggc tgtcggtaga taaaaacggc aaattggtca
     9481 taactgccga atcgaatgcc ggcaatccca tgcgcagcgg cctaaagcca ctgttgacca
     9541 tcgatgtctg ggaacacgcc tattacatcg atcaccgtaa tcgtcgtcct gatgccattg
     9601 cggccttttg gaaagtactc gattggtcgg tggtagaagc tcgcttcgaa aacaagctct
     9661 aagatttacc cggtcaccgg ataagccccg aggtatgttc gagcattaaa ttgccgggcc
     9721 atgaaaccac cgtatactat caccccgttg atactgaggt ctgtgtcttc tatttcggaa
     9781 aaactgggag aggtcaatgc cagtcgtttc cgttggcctt cgtctgcctg gagaaagcgg
     9841 aatactttgt taaaggtgca tgcctccttg cgtttggaag gaaataaaat gagtctgcgg
     9901 caggtcaagg ctgttcagga acaaagacag gtcaaggcag atccccggga tatcctggaa
     9961 gctcaaaatg ccctgagggt ttacgatgag ctttcactga tggatccctt ttcgtccaaa
    10021 gactttctta aaacgcataa aatgctgatg aacggtcttg tggagcttcc cgggaagttt
    10081 cgcaagcgcg aaatcggtat agtgcacggc gataatgtgg ctcacatcgc tcccccggct
    10141 aaaaagctga cttatttgat gcaggacttg tttcgctatc tcaaagacag cgaagagcct
    10201 atgttgataa aaagttgtgt ttttttctat gaaacggaat ttattcatcc ctttctcgac
    10261 ggaaacggca gaatggggcg tctgtggtgt agtttgttgt tgtcgaggat aaatagcctt
    10321 tttatgtaca taccattgga gcattatttg ttggaatcca gagacgaata ttacaaagcc
    10381 ctggctataa gcgacagtaa gggagcttcg acggcttttg tagagtattt gctccaaaag
    10441 attgaacagg ccttcaacga ttttttaatt gaaagaaaag tgctatcaaa aaacagccat
    10501 atatatgagg aaaacaaaaa aaacgcagaa ccaaaaaaag cgtgaatact tcgaaatttt
    10561 gaataccatt tccaagggta ggcaccggac caatatttta aagaaaccgg aacaaagcgc
    10621 cctggcttat ttggtcaaac gcattcccgc ctgggtaagt tcggacatgc tgaccttttt
    10681 tggtttttgt ggtagtttgc tgacagctgc atcctttgtg atggcagctt attggagccg
    10741 gctttatttg ttgttgggta tattggggtt tattattaat tggtttggcg attctttaga
    10801 cggaaggctg gcttattatc gcaacaaacc ccgaaagtgg tacggattta gcctcgacat
    10861 cacggtagat tggctcacaa ccgtgttgat aggctccggt tattcgcttt atacagaggg
    10921 ttttgctaag tttttcggat tggcttttgt ggtaatgtac agctgggcca tgatcaccac
    10981 cctgatgcgt tataaggtaa ccgaccaata ttccatcgat accggtattc ttggccctac
    11041 cgaagtgcgg gtagtgattt cattaattct gctggcagag gtattcatca aagattcaat
    11101 agtttatacc ggagcactgg cttgtgtggt tttattggtg gtgaatgtaa taagcaccgt
    11161 agaattgttg attgttgcag ataaacgcga caaagacgaa aaagaatcaa acgccggcag
    11221 caaaacagaa ggttaacttc tacatacata caacaaggta ttctattgaa aacacatgtg
    11281 gtagatataa gcgattttga gcagttttct cctgtgttta gaggtaaatt gggaaatctg
    11341 ctggccggac agattatcaa attattcgct ctcgacaagg tcaatgctct gtatcaacgt
    11401 tgttgcgatc acacgggagc agctttcgct tctgcattat tacgagatct tggggtgcaa
    11461 taccgcatag gaaacccgga tcgccttaaa caactgcccg aaggggcttt tattaccgta
    11521 tccaatcacc cttacggagg cctggatggc atcatgctaa tcgatttgat ggctcatatc
    11581 cggcgggatt ataagctaat ggtcaatgag attttatctt tgatcgaggc tatggatgtg
    11641 aattttattt ccgtccggcc tcagggcaac aaaaaactgc cacccacaac caacctggat
    11701 ggtgtacgcg aaaccattag tcatttgcgg aaagggcatc ccatgggttt tttcccttcg
    11761 ggagcagtgt ctgattttag cttcagaaac cgacgagtac aggaccgccc ctggcaggac
    11821 agtgtgatta aactcattaa aaaagccaaa gtgcccatat tgcccatccg tttcttcgac
    11881 ggtaattctc ccttttttta ttttctgggc accatcaact ggcggattcg tctgattcgc
    11941 atgcccagag aggtgttcaa caaaagcaca cagtttcccc gtctgggtat aggcaagcta
    12001 atcaccgtcg aacaacagtc ccgccaccgg aacaccgaag atttcggcgc tttgctgcgc
    12061 agctcggttt acgacatgcc cgtacccgcc caatttacac ccagagagcg tgtttttttg
    12121 gacgggtaag ttctttgctt aactgaacta gaaagaaaat cttgaaagc
//
LOCUS       DAOSTD010000054        12160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_5236_length_12160_cov_16.516811, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000054 DAOSTD010000000
VERSION     DAOSTD010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12160
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5236_length_12160_cov_16.516811"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PLI37_04930"
     CDS             <1..765
                     /locus_tag="PLI37_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58388.1"
                     /translation="YAHLFYRFLYNYHDTPPVVDSKGVPVATSLMAENRRSYQYLGSW
                     CLNLINAEYPAWKHKIDAQNEAGEATLYELSSTTYESSETTTYRFKDGFSVSNTGGKN
                     YATGSSNTVKYSANVEYTITIPQSLSVEKVVFYGYDNYDVDAYVSMLNGVSYTSTDYV
                     FPAKVGGTATFVKHTFDLGSAPATGTLAFKLGSKQCCLIISLYCTRPSGVREVVEIKA
                     PEPVKRLLNGQIVIVKEDKIYNMSGQQLKTVELDNQ"
     gene            860..1648
                     /locus_tag="PLI37_04935"
     CDS             860..1648
                     /locus_tag="PLI37_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF108 domain-containing protein"
                     /protein_id="HOQ58389.1"
                     /translation="MKKLVIFGSGYLANIVADAYLDGLLPEYELVGVYSRTIDKAKRL
                     ARKIAQSGRDCRPCVTLNALLSLKPDYLVETASPAAMKRLALPTLNNGTSIITLSIGA
                     FADTAFYEQVKQSAAANDVRVYIASGATGGFDVLRTATLMGQASANFLNIKGVRALRR
                     SSFYEPGMETEAKTVFTGTAREAIDVFPTNLNVAVAASLATVGPENLQVSMISTPEFT
                     GDTQRVEIKNKEVHAVVDVFSATPAIAAWSVVSTLQNIVSPIVF"
     gene            complement(1645..2541)
                     /locus_tag="PLI37_04940"
     CDS             complement(1645..2541)
                     /locus_tag="PLI37_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ58390.1"
                     /translation="MFQKLVAIEPVSLIPSAEKKLSDFAKEVVLYDTIPDSDREIARR
                     IGDADAVLLSYTTHLGKGALEQCKHLKYIGMCNSLYSPESANVDIYYANSRGITVTGI
                     RDYGDEGVVEYVISELVRCLHGFDQVPWDGMAREITGLKAGILGLGKSGGMIADALHF
                     FGAEISYFARSEKEAARQKGYRFLPLNDMLRESEVVCCCLNRNTVLMQARQFEALGNK
                     KILFNTGLSPAWEEAPFLHWLEGDNLCFCDTVGALGGPHLLDHPKVRCMQVSTGRTRQ
                     AFVRLSEKVLQNLTDFTGIKPD"
     gene            complement(2748..3272)
                     /locus_tag="PLI37_04945"
     CDS             complement(2748..3272)
                     /locus_tag="PLI37_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOQ58391.1"
                     /translation="MISSLSFKALNLSFTNYRTYLFGAAFVAGNLLLPQLCHLMPNGG
                     LIWLPIYFFTLIAAYKFGLKIGLFTAALSPLVNYLLFGMPPLGMLPIILIKSGLLAFA
                     AAFVASKTNKLSLLHLLVVILAYQVLGGLAEWGLSGSFAAALQDFKLGLPGLLVQLFG
                     GWLVLKALAKYELK"
     gene            complement(3409..3672)
                     /gene="rpmA"
                     /locus_tag="PLI37_04950"
     CDS             complement(3409..3672)
                     /gene="rpmA"
                     /locus_tag="PLI37_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOQ58392.1"
                     /translation="MAHKKGVGSSKNGRESESKRLGVKIFGGQFAKAGNILVRQRGTV
                     HHPGDNVGIGKDHTLFALVDGTVTFKRKKNDRSFVSVLPQEEA"
     gene            complement(3693..4007)
                     /gene="rplU"
                     /locus_tag="PLI37_04955"
     CDS             complement(3693..4007)
                     /gene="rplU"
                     /locus_tag="PLI37_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOQ58393.1"
                     /translation="MYAIVDIAGQQFKAEAGRRLYVHRLEAEQGSVVEFDKVLLVDNE
                     GAVQVGTPVVEGAKVVAEVLSHLKGDKVIVFKKKRRKGYRKKKGHRQYLTEVKINEIV
                     VA"
     gene            complement(4139..5071)
                     /gene="trxB"
                     /locus_tag="PLI37_04960"
     CDS             complement(4139..5071)
                     /gene="trxB"
                     /locus_tag="PLI37_04960"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOQ58394.1"
                     /translation="MEHVRCLIIGSGPAGYTAGIYAGRAGLKPLLFEGMQIGGQLTTT
                     TEVENFPGYPQGVSGFQLMEDLRNQAQRFDCECRSGTITAADLSQRPYVLTVDDETKI
                     SCDSLIIATGATAKYLGLADEQKYAGMGVSACATCDGFFYRQKTVAVVGGGDSACEEA
                     LYLSTLASKVYVVVRKNYLRASKIMQERTLNNPKISVLFEHEVVGLYGDNGVEGAHML
                     KKRGQTGESRFDLPIDGLFLAIGHKPHSDIFAPYLKVDKTGYIITEPNTPKTSLPGVF
                     AAGDVADSVYRQAVTAAASGCKAAIEAERYLNEL"
     gene            complement(5076..5726)
                     /locus_tag="PLI37_04965"
     CDS             complement(5076..5726)
                     /locus_tag="PLI37_04965"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-like putative outer membrane lipoprotein
                     chaperone"
                     /protein_id="HOQ58395.1"
                     /translation="MSGPKKTLPGLLFLLLGIWAIQAQNQALTILEKAGEAYNKTSGV
                     HAEFSIEMFESGGESAAQIQGQIQLKGDKFKLDVGEMITWFDGKNQWFYLVNIQEVNL
                     SNPAPEDLMMINPVMVFQLYKYGFDTRYAGEKKLGTKIAQQVELLPQEPYSDIQRIEV
                     YFDKQTYQPLRIRIRNKDQSGSLINIDKYITDQKYPDAMFVFQQKAYPGAVVIDLR"
     gene            complement(5730..8180)
                     /locus_tag="PLI37_04970"
     CDS             complement(5730..8180)
                     /locus_tag="PLI37_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOQ58396.1"
                     /translation="MAVKKKASKGFLSGIHDSGFIRFWTNERTRFISGLLLLMAMIYF
                     SILLISFFFTGAADQSLVKDLSFSELGGVKGVGNWAGTFGAWLSNIIMTKWMGISSLF
                     IAVFGLIAALKLMRVRTPNLGKVFFVCCFALIWLSLFFGFFFIREESSQFLFLGGAHG
                     YYISRWLQESIGWIGTFLFLLLSLIVFLVLTSKKTIPLLRSLFKMDYLKNKQAELADT
                     MEEEAATEGPKDLGYTKPPLADDPQTEENGELPSTNEEEEKSPGQKTRAGKDIDMDII
                     VPKGDENINADEESDDEELLEQLRKKKNEEYDALAELGEYDPRAELSKFKNPPPEILK
                     QYDDRTVVNHQEQTENKRKIVDVLESFGIRISSIQATVGPTVTLYEIVPEPGIRITKI
                     KGLENDIALNLAALGIRIIAPIPGKGTIGIEVPNRDPKLVSMFSLVKSRKFQETKYDL
                     PVVLGKTIMNEVFMFDLCKMPHILVAGATGQGKSVGLNALITSLLYKKHPAELKFVLV
                     DPKMVEFSIYSPIEKHYLAKLPGDENKAIITDYTKVVQTLHSLTTEMDDRYKLLNMAH
                     TRNIKEYNAKFIARRLNPEKGHKYMPYIVIVIDEFGDLIMTAGKEIELPIARIAQKAR
                     AVGMHMVIATQRPTTNIITGTIKANFPARLAYKVSSATDSRTILDANGANQLIGKGDS
                     LFSQGNDMTRIQCAFIDTPEVIDMVEYISSQRGYPTAFELPEYVDESSGGGLSDVDLS
                     LRDPLFEEAARLIVIHQQGSTSLIQRKFSIGYNRAGRIVDQLEAAGIVGPSEGSKARQ
                     VLVDDEHHLDLLLSNLNP"
     gene            8313..>8938
                     /locus_tag="PLI37_04975"
     CDS             8313..>8938
                     /locus_tag="PLI37_04975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOQ58397.1"
                     /translation="MTRIKSTITKEEIQKYPQAEFRGKIHLFQDANIPEWVIRKLNAQ
                     QVVGFDTETRPAFIKGRRNKVSLMQLACEDECFLFRLKPSGLAPELLSFFANPRVVKV
                     GLSSKDDFHMLRASYAEFDPQNVVELQSYVKQYGIEEMSLLKIYAILFKERISKSQRL
                     SNWDAEQLNAAQLQYAALDAWAVRRIYLHLSSLPSSQNKALITESETK"
     assembly_gap    8939..9038
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9039..9633)
                     /locus_tag="PLI37_04980"
     CDS             complement(<9039..9633)
                     /locus_tag="PLI37_04980"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ58398.1"
                     /translation="MSEGFGLYLHIPFCKSKCAYCDFYSLCGTEGKTVFLDCLSKESG
                     SEKQYIDGNQLSTIYFGGGTPSLLSANDYKLLFESIGREYDIGRCTEITLEANPDDLS
                     PAYLKSISGLPFNRISIGIQSFSDRELRALGRRHDASQGIEAVGRCREAGLNNISIDL
                     MFGIPEQNELSLQSNIEQALKLEVPHISVYMLSLEPGS"
     gene            9746..11905
                     /locus_tag="PLI37_04985"
     CDS             9746..11905
                     /locus_tag="PLI37_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOQ58399.1"
                     /translation="MKIYQTNEIKNLAIVGNAGAGKTTLAEAMLFEGGLVKRKGNINA
                     NNTVCDYFPVEHEYGYSVYSTVFHIEWKGKKLNLIDCPGSDDFVGGAVTALNMTDAAL
                     LVLNTQYGIEVGTQNSFRLADKYNKPIVFVLNQLDHEKAEFDRALEQLKEAFGSKVMP
                     LQFPVETGPGFNAIVDVLEQKLFTWKAEGGEPQVGEIPANLKAKAEAYHAALIEAAAE
                     NDEALMEKFFEDGTLNSEEIRKGIQLGLAAKGIFPVVCVCGGNGVGVSRLLDFMKDIA
                     PSVLDVPAPKNSGGVEVAPDANAPCSLYFFKTSVEPHIGEVCYFKVMSGKVKEGDDLQ
                     NANRSSRERITQLFCTAGANRIKVEELQAGDMGATVKLKDLRTGNTLNAKGVEHKFDF
                     IKFPDPKYRRAVKPVNEADMEKMMTILNRMHEEDPTYIIEQSKELRQTIVHGQGEFHL
                     RTLKWIIENIDKLAITFAEPRIPYRETITKSARADYRHKKQSGGAGQFGEVHLIVEPY
                     TEGMPIPEMFKFGGQEYKVTNRDTQEYDLEWGGKLVFVNSIVGGTIDARFLPAILKGI
                     MGRMEQGPLTGSYARDVRVIVYDGKMHPVDSNEISFMLAGRNAFSAAFKDAAPKILEP
                     IYDVEILVPGDRMGDVMGDLQGRRAVIMGMQSEKGLEKLSAKVPLKEMSNYSTSLSSL
                     TGGRASFTMRFASYELVPADVQDKLLKEYEAQQQEKE"
BASE COUNT         3125 a         2843 c         2918 g         3174 t
ORIGIN      
        1 tacgcgcatc ttttttaccg tttcctctac aactaccacg acactcctcc cgttgtcgac
       61 agtaaggggg ttcccgttgc cacctcgtta atggccgaaa accgtaggag ttaccagtat
      121 ctcggcagtt ggtgcctgaa tctgattaat gcggaatatc ccgcttggaa acataagatc
      181 gatgctcaga atgaagccgg tgaggcgaca ctttacgagc tgtcctccac aacctacgaa
      241 tccagcgaga ctacgaccta tagatttaaa gacggtttca gcgtctcaaa tacgggaggt
      301 aaaaactacg caacaggaag ttcaaacaca gtgaagtact cggcgaatgt ggaatatacc
      361 attaccatcc cccagagcct gtcggtagaa aaagtagtat tctacggcta cgacaactac
      421 gatgtggacg cctatgtcag tatgctgaac ggcgtttctt atacatcgac agactatgtg
      481 tttccggcga aagtcggcgg tacggcgacg ttcgtaaaac atacttttga cctagggtcg
      541 gctcctgcca ccggcactct tgctttcaag ctcggttcca agcaatgctg ccttatcatt
      601 tctctctatt gcaccagacc atccggcgta agggaggtgg tagaaatcaa agcacctgag
      661 ccggtcaagc gcctactgaa tggacagatt gtaattgtca aagaagataa aatttacaat
      721 atgtccggac agcagctaaa aacggtagag ttagataatc aatgagccgg gcttttgagg
      781 tggttgtact tgaatttttc caggaagttc acttgatcgc ggctggcaaa tttgccctaa
      841 aggtcgtatc tttgcgggca tgaaaaaatt ggtcattttt ggaagcggct atttagccaa
      901 tattgtagca gatgcttatt tggatgggct tttgcctgag tatgaattgg tgggtgttta
      961 ttcccgtacg attgacaagg ccaaacgtct ggcaagaaaa attgctcagt cgggacgcga
     1021 ttgccggccc tgcgtcaccc tcaatgcttt actgtctctg aagcccgact atctggtcga
     1081 aacggcctct ccggcagcta tgaagcgact ggccctgcca acgctcaata acggtacttc
     1141 catcataacc ctttccatcg gtgcctttgc cgacaccgct ttttatgagc aggtgaagca
     1201 gagtgctgcc gccaacgatg tccgggtgta tattgcttcc ggcgccacag gtggctttga
     1261 tgtcttacgc acagccactc tcatgggtca ggcctcagca aattttctta atataaaggg
     1321 cgttagagcc ctcagacggt cgtctttcta cgaaccgggc atggaaacgg aagccaagac
     1381 cgtctttacc ggcactgccc gcgaagccat cgatgtgttt cccaccaact tgaatgtagc
     1441 tgttgccgca tcgctggcta cggtgggtcc ggaaaacctg caggtcagta tgatttcaac
     1501 ccccgaattc acaggcgata cacaacgggt ggaaataaaa aataaggaag tgcatgcagt
     1561 ggttgacgta ttcagtgcca caccggccat cgccgcctgg tctgtggtca gtaccctgca
     1621 aaacatcgta agcccgattg tcttttagtc gggcttaatc cctgtaaagt cggttaaatt
     1681 ttgcaagacc ttttcgctca agcggacaaa ggcctgacgc gtgcgccctg tggatacttg
     1741 catacaacgt accttggggt gatcgagcag gtgagggcca cccaaggcac ccaccgtgtc
     1801 gcaaaaacaa agattgtcgc cctctaacca atgcaaaaag ggcgcttctt cccaggccgg
     1861 tgaaagccct gtgttaaaca gtatcttctt gttgcccaaa gcttcgaact ggcgcgcttg
     1921 catcaggacg gtgtttcggt ttaagcaaca gcacaccacc tcactctctc ttagcatatc
     1981 gttcaagggc agaaaacgat agcctttctg ccgggctgct tctttctccg accgggcgaa
     2041 atagctgatt tccgcgccaa aaaaatgcaa ggcatcggcg atcatgccgc ccgacttgcc
     2101 cagccccaga atgcccgcct tcaaacccgt aatttcacgc gccatgccgt cccagggtac
     2161 ctgatcgaag ccgtgcaggc agcgcaccaa ctcactgatg acgtattcca ccacgccttc
     2221 gtcgccatag tcgcgtattc ccgtcaccgt gatgccccgg ctgttggcgt aataaatatc
     2281 cacattggca ctttcgggcg aatacaagga attgcacatt ccgatgtact tcaggtgttt
     2341 gcactgctct agtgcccctt tcccgagatg ggtggtatag ctcaacaata cggcatcggc
     2401 atcgccgata cgtctcgcga tttcccggtc ggaatccgga atcgtatcgt ataaaacaac
     2461 ttctttggca aaatcagaca gttttttttc ggccgaagga atcagactga ccggctcaat
     2521 ggccacaagt ttttggaaca taggctggaa ttctaaaacg tttataatgc tgcaaagata
     2581 gcatttaaaa ctcagaccgg agttgttgat ccggctgtag ggcggagttg agcataacat
     2641 caaaaaagtg gccatcaaat cgacagccac tttaatttct tgtgtaaaca aaccggttgt
     2701 ttcagtttgt actttttatt ttagcttgcg ctttttattt cagttcttta tttcagttcg
     2761 tattttgcca atgctttcag caccaaccag ccaccgaaga gctgaaccag tagaccgggc
     2821 aggcctaatt tgaaatcttg caatgcggcg gcaaaacttc cgctcagtcc ccattcagcc
     2881 agacctccca atacttgata agccagaatt accaccagca aatgcaacaa tgaaagtttg
     2941 ttggttttgg aggcaacaaa ggcagcggca aaagccagca aacccgattt aatcaggatg
     3001 atgggcagca tacccagggg cggcatacca aaaagcaggt aattcacaag aggagacaag
     3061 gccgccgtga ataagccgat cttcaggcca aatttatagg cggcaatcag tgtgaagaaa
     3121 taaatgggca gccagatcaa accaccgttg ggcatcaagt ggcaaagctg aggcaaaagc
     3181 aggttgccgg ctacaaaggc ggcaccgaac aaataagtgc gataattggt aaagcttaaa
     3241 ttgagggctt taaaactcag tgaggatatc atatcattga aatttatttg ttatacaaac
     3301 gattgcaatt gcttaaacgg acgacaaata taagcaagcg aacgaaacaa cacaaaaaaa
     3361 acgggcaata atttgcccgc tttaagtgat attatcgttt aaaaaccttt atgcttcttc
     3421 ctggggaaga acagaaacaa aggaacgatc gttttttttg cgcttaaaag ttaccgtgcc
     3481 gtcgaccaag gcaaagagag tatggtcttt accgatacct acgttatcgc ccggatgatg
     3541 caccgtgccg cgctgacgaa ccaaaatatt tcctgctttg gcaaactgtc cgccaaagat
     3601 cttaacgccc aatcgtttgc tttccgattc gcggccgttc ttggaactac caactccttt
     3661 tttatgtgcc ataatctgta aaatttaaag gattaagcga ctacaatttc gttgattttc
     3721 acttcggtca ggtattgacg atgacctttt ttctttctgt agccttttct tcttttcttc
     3781 ttgaagacaa ttaccttgtc tcccttgagg tgagaaagga cttcggccac tactttggca
     3841 ccttctacga caggtgttcc cacctgtacg gctccttcgt tgtcgaccaa aagaacttta
     3901 tcaaactcta ccacagaacc ttgttctgct tccaggcgat gcacatacag cctgcggcct
     3961 gcttcggcct tgaactgttg ccccgcaatg tctacaattg catacattaa ctaactaatt
     4021 tacaagttat tcttcggaag gtgggatgcc agggcattgc cgctttctac cttacacgaa
     4081 atcgagccgc aaaagtagct ctttttttta attctgccaa gttaaaactt gaaatatttt
     4141 acagctcgtt caaataacgc tcggcttcga tggcagcttt gcaacccgaa gctgctgccg
     4201 taacagcctg ccggtaaacg gaatcggcca cgtcgccggc ggcaaaaaca ccgggcagac
     4261 ttgttttggg cgtgttgggt tcggtgatta tataacctgt cttgtccact ttgagatagg
     4321 gagcaaagat atcggaatga ggtttatgtc ctatggctaa aaacaaaccg tctataggca
     4381 gatcaaaacg gctttcaccg gtctgtcccc gttttttgag catatgggcg ccctccactc
     4441 cgttgtctcc gtaaagaccg accacctcgt gttcaaataa tacgctgatt ttgggattgt
     4501 tcaacgttct ttcctgcatt atcttagagg ctctcaggta attcttgcgc accactacgt
     4561 acactttcga ggccagagtc gacagataaa gggcttcttc gcaggccgaa tctcctccgc
     4621 ccaccacggc cacggttttt tgacggtaaa aaaagccgtc acaggtggcg caggccgaaa
     4681 cacccatacc cgcgtatttt tgttcatcgg ccaggcccag gtatttggca gtggctccgg
     4741 tggcaatgat cagactatcg catgagattt tagtttcgtc gtctacagtg agcacatagg
     4801 gacgctgcga gaggtcggcc gctgttatcg tgccgctcct gcattcacaa tcgaaacgtt
     4861 gagcctggtt gcgcaggtct tccataagtt gaaaaccgct cactccctgt gggtagccgg
     4921 gaaagttttc tacttcggtg gtagtagtca attgtccgcc aatttgcatg ccttcgaaca
     4981 acaaaggttt tagtccggcc cgaccggcgt aaattccggc ggtatagccg gcagggcccg
     5041 atccgataat caggcaacgt acatgttcca taattttacc tcaaatctat gaccacggca
     5101 ccgggatagg ctttttgctg aaagacaaac atagcgtccg ggtatttttg gtcggttata
     5161 tatttatcaa tattaatcaa agatcccgat tggtctttat tacggatacg aatgcgcagg
     5221 ggttggtaag tttgcttgtc gaaatagacc tcaatacgct gaatatccga atagggctcc
     5281 tgagggagca attccacctg ttgggcaatt ttcgtgccaa gctttttttc tcctgcataa
     5341 cgggtgtcaa aaccgtattt atatagctga aacaccataa caggattgat catcatcagg
     5401 tcttcgggag caggattgct taggtttact tcctgaatat ttaccaaata aaaccattga
     5461 ttcttaccgt cgaaccaggt aatcatttcg cctacatcca gtttgaattt atcgcctttc
     5521 agttgaattt ggccttgaat ttgggcagcg ctttctccgc cggattcaaa catctctatg
     5581 ctgaattcag cgtgtactcc gctggttttg ttgtatgctt cgccggcttt ttccaggata
     5641 gtaagggcct ggttttgagc ctggatggcc catatcccca ggagtaaaaa caacaagccg
     5701 ggcaaggttt tcttcggtcc tgacatgttt taaggattta aattggataa gagcagatcc
     5761 aggtggtgtt cgtcgtctac cagtacctgc cgggctttgc taccttcgct gggaccgaca
     5821 atgccggcgg cttccagttg gtcgacaatg cgtccggccc ggttgtagcc tatcgagaat
     5881 ttgcgctgaa tcagcgaagt cgatccctgc tggtgaatca cgatcagacg ggcggcttct
     5941 tcgaaaaggg gatcgcgcag gctgaggtct acatcgctca ggccgcctcc gctgctttcg
     6001 tccacgtatt cgggcagttc aaaggcggtg ggatatccgc gctggctgct gatgtattca
     6061 accatatcga tgacctcggg cgtatcaata aaggcgcact ggatacgggt catatcgttg
     6121 ccctgtgaaa acagggagtc tcccttgcct ataagctggt tggcgccgtt ggcgtcgaga
     6181 atggtcctgg agtcggtggc ggaagatact ttgtatgcca gcctggcagg gaagttggcc
     6241 ttgatggttc cggttataat attggttgtg ggacgctgag tggcaatcac catgtgcata
     6301 cctacggcac gggctttttg ggcaatacgg gcaatgggga gttcgatctc tttgccggcc
     6361 gtcataatca ggtctccgaa ctcgtcgata acgatgacaa tgtaaggcat gtatttatgg
     6421 cctttttcgg gattgagccg gcgagctata aacttggcgt tatattcctt gatattgcgg
     6481 gtatgagcca tattcaacag cttgtaacga tcgtccattt ctgtggtgag cgaatgcagg
     6541 gtctggacga ctttggtgta gtcggtgatg atggccttgt tttcgtcacc gggcagtttg
     6601 gccaggtaat gcttttcgat gggagaatag atactgaatt ccaccatctt gggatcgacc
     6661 aaaacaaact tgagttccgc gggatgtttt ttgtacaaaa gggaagtgat caatgcgttc
     6721 aaccccaccg attttccttg tccggtggct ccggcaacca ggatatgcgg cattttacag
     6781 aggtcgaaca taaacacttc gttcataatg gttttaccca gcaccacggg caggtcgtat
     6841 ttggtctcct gaaatttgcg cgatttcacc aggctgaaca tagataccag tttaggatcg
     6901 cggttgggca cttctatacc gatggtgcct ttgcccggaa tgggagcaat gatgcgaata
     6961 ccaagggctg ccagattcag tgcgatatcg ttttcgagtc ccttgatctt ggtgatgcga
     7021 attccgggtt ccggtacgat ttcgtacaag gtgacggtgg gccccaccgt ggcctgaatg
     7081 ctggaaatcc gtatgccgaa actttcgagt acatcgacaa ttttacgctt gttttccgtt
     7141 tgttcctggt ggtttaccac cgttcggtca tcgtattgct ttaaaatttc cggaggcgga
     7201 tttttgaatt tgctcagctc ggccctaggg tcgtattcgc ccaattcggc cagggcatcg
     7261 tattcttcgt ttttcttttt gcggagttgt tccagcagtt cctcgtcgtc tgattcttca
     7321 tcggcattga tattctcgtc gccttttggt acgataatat ccatatcgat gtccttaccg
     7381 gcccgggttt tctgtccggg ggatttttcc tcttcctcat tggtagaagg caattctcca
     7441 ttttcttcag tttggggatc gtcggcaagg ggcggtttgg tataacccaa gtctttcggg
     7501 ccttccgtgg cagcttcttc ttccatggta tccgccaatt cggcctgctt attttttaaa
     7561 tagtccattt tgaagaggga gcgcagcagg ggaatggttt ttttgctggt gagtactaaa
     7621 aagacgatca aactgagcaa aaggaacaga aatgttccta tccaaccaat gctttcctga
     7681 agccagcggc tgatgtaata gccgtgagct cctcccaaaa acaaaaattg gctgctttct
     7741 tcgcggataa aaaagaaacc gaaaaacaag gagagccaga tgagtgcgaa acagcacaca
     7801 aaaaaaactt tgcccagatt gggggtacgt acccgcatca gttttaaggc ggctatcagg
     7861 ccaaagacgg ctataaacag tgaagaaata cccatccatt tggtcatgat gatgttcgac
     7921 agccaggcgc caaaagtccc ggcccagttg ccgacaccct ttactcctcc gagctctgaa
     7981 aaagacaggt ctttgaccag actctggtcg gcggctcccg taaaaaagaa tgaaatcagc
     8041 aggatgctaa agtaaatcat ggccatcagc aacagcaagc cgctgataaa acgagtccgc
     8101 tcattagtcc agaagcggat aaaacccgaa tcgtgtatgc ccgacaaaaa cccttttgaa
     8161 gctttctttt taactgccat aaagcaaaat gttacctctt tggaattagg tggcaaatat
     8221 acagaatttc ttacggagcc gcaggggttt ttacaaataa agccgtattt ttgtcttcct
     8281 ctttacccgg ggacggtaag aaaccttaca gaatgaccag aatcaaatcg accatcacca
     8341 aagaagaaat acagaaatat ccccaagcgg aatttcgggg caagatacat ttgtttcagg
     8401 atgccaacat ccccgaatgg gtaatacgga aattgaatgc tcaacaggta gtgggtttcg
     8461 acaccgaaac caggccggct tttatcaaag gccgcagaaa taaagtatcg ttgatgcagc
     8521 ttgcctgcga ggacgaatgt tttttatttc gcctgaagcc ttcgggcctg gctcccgaat
     8581 tgttgtcttt ttttgctaac ccccgcgttg tcaaggtcgg cctctcgtcc aaagacgatt
     8641 tccatatgct gcgggccagt tatgccgaat ttgatcccca aaatgtagta gagctgcaaa
     8701 gttatgtgaa gcaatacggc atcgaagaga tgagtttgct caagatctat gccattctgt
     8761 ttaaagagag gatttccaaa tcgcagcggc tttccaactg ggatgcggaa caactcaatg
     8821 ccgctcagtt gcaatacgcc gctttggatg cctgggccgt caggagaatt tatttacatc
     8881 tgtcgagctt accgtcgtcc caaaacaagg ctcttattac ggagtccgaa acgaaatann
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg ctcccgggct ccagactgag
     9061 catatagacc gaaatatgtg gaacttcaag tttcagcgct tgttcaatat tgctttgcag
     9121 gctgagctca ttttgttccg gaataccaaa catcaggtcg atgctgatgt tgttcagtcc
     9181 ggcctcgcgg caacgaccga ccgcctcgat gccttgcgag gcatcatgcc tgcggcccag
     9241 ggcccggagt tcccggtcgc tgaaactttg gatgcctata ctgattctgt tgaagggcaa
     9301 gcctgaaata gatttcaggt aagcgggact aagatcgtcg ggattggctt ccagcgtaat
     9361 ttcggtacaa cggccgatgt cgtactccct gccgatgctt tcaaaaagga gtttgtaatc
     9421 gtttgcactc agcagcgaag gagtgccgcc tccaaagtag atcgtcgata gttggtttcc
     9481 gtcaatgtat tgcttttcgg agcccgattc tttggacagg caatccaaaa aaacagtttt
     9541 accttctgtg ccgcaaaggg aatagaaatc gcagtaagcg catttgcttt tgcaaaaggg
     9601 aatatgcagg taaagaccaa aaccttcgga catagtggaa aatggctgag acaaagttag
     9661 ttcaaattcg gttgtgttgt tgtttctttt tagaattatt tctatcttgc gccacttttt
     9721 tgaaccttaa aaacagagaa ataagatgaa aatttatcag acgaacgaaa tcaaaaatct
     9781 ggctattgta ggaaatgccg gagccgggaa aaccactctc gccgaagcta tgcttttcga
     9841 gggtggatta gtcaaacgca aagggaatat caacgcgaac aacacggtat gtgattattt
     9901 ccccgtagag cacgagtatg gatattccgt ttattcaacg gtgtttcaca ttgaatggaa
     9961 gggaaagaaa ctcaatttga ttgattgccc cggatcggac gatttcgtag gcggagccgt
    10021 aactgcacta aacatgacag atgctgctct gctggtcctg aacacacaat acggcatcga
    10081 agtgggtacg caaaacagtt ttcgtctggc tgataagtac aataaaccaa tcgtcttcgt
    10141 gttgaatcag ttggatcacg aaaaggccga attcgacaga gctttggagc agttgaagga
    10201 agctttcggc tccaaggtta tgcctctgca attcccggtt gaaaccggcc ccggattcaa
    10261 cgctatagtg gatgtgctgg agcaaaaact ctttacctgg aaagccgagg gcggagaacc
    10321 tcaggtagga gaaattccgg ccaacctcaa agccaaagcc gaggcttatc acgctgcgtt
    10381 gattgaagct gctgccgaaa acgacgaagc cttgatggag aaattcttcg aagatggcac
    10441 cctcaatagc gaagaaattc gcaaaggaat acaattgggt ctggccgcca aaggaatttt
    10501 ccccgttgta tgtgtctgcg gtggcaatgg tgtaggagtc agtcgtttat tggatttcat
    10561 gaaagatatt gcacccagtg ttttggatgt gccggcaccc aaaaacagcg gaggtgttga
    10621 agtcgctccc gatgccaatg ctccttgcag tttatatttc ttcaaaacat cggttgagcc
    10681 gcatattggc gaagtctgct atttcaaagt aatgagcggc aaggtaaagg aaggcgacga
    10741 tttacaaaat gccaatcgca gttccaggga acgtattacc caattgttct gtacagccgg
    10801 agccaacagg ataaaggtgg aggaattaca agccggcgac atgggagcta cggtcaaatt
    10861 aaaagatctc agaacaggca atacgctcaa cgccaaaggt gttgaacata agtttgactt
    10921 tattaaattc ccagatccta agtaccgccg tgcggtgaag cctgtcaacg aggccgatat
    10981 ggaaaaaatg atgaccatat tgaatcgtat gcatgaagaa gatcctacct atattatcga
    11041 acaatcaaag gaattaaggc aaaccatcgt gcacgggcag ggagaatttc atctccgtac
    11101 gctgaaatgg ataatagaaa acatagacaa attggccatc acttttgcag aacccaggat
    11161 tccatatcgc gaaaccatca ctaaatcggc ccgggccgat tataggcaca aaaaacaatc
    11221 gggtggtgcc ggacagtttg gcgaagtgca tctgattgta gaaccctata ccgaaggcat
    11281 gcctattccc gaaatgttca agtttggagg ccaggaatat aaagtcacta atcgcgatac
    11341 tcaggagtac gatctggaat ggggtggtaa gctcgtgttt gttaacagta ttgtgggtgg
    11401 taccatcgat gcccgttttc tgccggccat tctcaaggga atcatggggc gcatggaaca
    11461 gggccctctg accggctctt atgcccgtga cgttcgtgtt attgtgtacg atggtaaaat
    11521 gcacccggta gattccaacg aaatttcgtt tatgctggcc ggccgcaatg ctttcagtgc
    11581 cgcctttaag gacgctgctc ccaagatttt ggaacccatc tacgacgtag aaattctggt
    11641 acccggcgat cgtatggggg acgtaatggg tgaccttcag ggcagacgcg ccgtcattat
    11701 gggtatgcag agcgaaaaag gtttagaaaa gctctcggcc aaagtacctt tgaaagaaat
    11761 gtcgaattat tccacttcgc tgagttcgct caccggaggc agggcttcct ttacgatgcg
    11821 ctttgccagt tacgaactgg tacctgccga tgtacaggat aaactactca aagaatacga
    11881 agcacagcag caggaaaagg aataaaactt aattaaatta ttagtacagc ccgggacagt
    11941 gattttcgaa aatactgttc cgggttgtta cattttcagg agcttaagtt ttttttgtta
    12001 atttacgttt ttatacacgt taatgttttt taatcattga aatgtattaa ttttttaacg
    12061 tattttcgtt cattactaag tattatccat gaaaaagtcg gtcatctggt tgttgattgg
    12121 tgtaatggca atgacctttg ctgcgctcat agccttgcag
//
LOCUS       DAOSTD010000055        11619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_5505_length_11619_cov_13.473712, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000055 DAOSTD010000000
VERSION     DAOSTD010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11619
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5505_length_11619_cov_13.473712"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            880..2172
                     /locus_tag="PLI37_04990"
     CDS             880..2172
                     /locus_tag="PLI37_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ58400.1"
                     /translation="MIEEFSFENYKSFKEMNTLNMTAAKLKSKYSKVDVDPTIQLDDD
                     VTLLKSKAIYGANASGKSNIVKAFGSFIRIVQHSVKDEQILEKHMAPFFLSTETFDKP
                     VFMQLVFRCEGVRYRYGFQADRERIHSEWLYATPGKREQPYFIRDNDTLIKINKKNFS
                     EADVVMKLIDNEKGNPIFRKNSLFLTSLAAFGVGKISKMLLNEIARFMVVDGLKHPGA
                     FDQTTASLEMPGMKKLVQTFLAMADAGINELKTIEVPDENVGESGNLAKKRKIVVAVK
                     DVYDANHKLFSTMPFPFSLFESEGTNKMYEISTFIINALREGRVLVIDEFDARFHPLI
                     TQKIVELFNSQGNKNGQLIFVTHDTNLLSADLLRRDQIDFVEKDTYGSSHLYTLVQFK
                     GVRDNDPFEKNYIKGKYGAIPFVGDFNNLFAHFEDAEA"
     gene            2418..5258
                     /locus_tag="PLI37_04995"
     CDS             2418..5258
                     /locus_tag="PLI37_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58401.1"
                     /translation="MIPYPYSKISLRKCCALFLSACLGAACFAQTTYDTIRSWNLAVY
                     SAQTLADLAADTERWNPMYKDGVLQRYSNKLPTDGLPLQANGQVIAEVEGLLVGRGIE
                     AGSLLLRHNMGSSHNGMQMQRIAPVSISGLRAGQRVTVTIRSSSSAPQGIAEVSNLSG
                     SCGPGTYPTTYFKTYAFDVVADGEASWTNSGGVVVQSVAVMEVSEDIREQVATPLIEV
                     DGKQVSLSCETPDAVLWYSLVDHADVLDYARLYTGPFELTRPCRFRAIAQKEGMRNSL
                     VADTNLVVAVTFPFAGRPRVLDPEALDRAAVATYMGSGSRYLINWRWLIDDPMQMSFN
                     VYRDGVRLNDSPISDRTNYMDYAGTATSEYTVEAVVAGEVVETAPVRVLPKGYLDIPL
                     NRPAGGTTESGDFYYIPGDCMVADVDGDKAYEVIMKWDPSNGKDNSQSGYTGNVLIDA
                     YRMDGTQLWRIDLGKNIRAGAHYTQLMVYDLDGDGRAELACKTAPGTIDGLGNYVLMG
                     DDDPRADYRTTVGSKSGMIISGPEYLTVFSGLTGEALATTAYRPPRDTISNWGDNYGN
                     RSERYLACVAYLDGERPSLVMVRGYYTAAFLWAVDFDGEKLSTRWLHSSIPGGIGAYG
                     EGAHSMATADVDGDGRDEIIFGACALDDDGSIIYRTGLGHGDALHVGDFIPDRPGLEV
                     MMVHEEVSAQYGVEMHDALTGEIISGRFAGSDVGRGLCADVDPTSRGHEYWSTASYDV
                     YDGAGTVLSTKRPTVNFRTYWDGDLQCETTERGVITKWQGRTQNAATLVDMPNKYGAG
                     TNVIKYTPCLQADIFGDWREEQIYYDNATMSHLWIFATPYVSNYRIPTLMHDHHYRMA
                     TVTQTAAYNQPPHLSFYLPDYVEYLTALPGSEAPYDLYSEGDASQGAKQVLSTVYYDL
                     LGRPIAEPERGFYIRENLYSDGSLSRVKCYKP"
     gene            5609..6541
                     /locus_tag="PLI37_05000"
                     /pseudo
     CDS             5609..6541
                     /locus_tag="PLI37_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
     gene            6563..8041
                     /locus_tag="PLI37_05005"
     CDS             6563..8041
                     /locus_tag="PLI37_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen translocase"
                     /protein_id="HOQ58402.1"
                     /translation="MIHKLADRLRGRNKPAKEGYRTAFKATSLFGGVQVVTVLTGLLK
                     SKLVAVWMGTTGFGILSLYNAAVNLIFSISNLGLQSSAVRDIAAASADKGKTLAIIKA
                     VNRWVLASGLLGAFLTVALSPRLSRWLFESDQYVGAFVLLSAAVLLMGLFNQNYAVLQ
                     GLRKLRLMARANIFGALAGFVCSVPVFYFFREQGLAWTVVISALASAVVSLFYVRKLG
                     LVLPKQDWHTSMVLGSDTVKLGIVMAISSIAVFMVQFVVKTYISHRGGLGDVGLYQAG
                     WGLNEQYLGLVFTAMAKDYFPRLSQVAGDHRKVRKSINEQAEIAILILAPMIIAMLVF
                     LPFVVRLLYSGDFDGIVPMTRWLLLGSLLKAGSWAISFVFLAKGHGKTYLFNELGIKF
                     FTLPTYMLGYRLFGLLGVGYAYVLCYGIYFLWVGIVAYRKYRLMYSGVFWRMLAFTTT
                     VLAAYLLMEPLMISGGLHYWLGIPLIVLLSIYSLRELKKRLF"
     gene            8202..8867
                     /locus_tag="PLI37_05010"
     CDS             8202..8867
                     /locus_tag="PLI37_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acyltransferase"
                     /protein_id="HOQ58403.1"
                     /translation="MSKKILIIGGEGNGGVVAACIEDMRLRHRILEWEVAGFINDYEQ
                     SVSGYPVLGGTGDVQDILRRHPDYYLMWAIHTIGRNYLIEKVFNKANIPAERLATIVH
                     PSAFVAPTAHLEPGVFVMSNCYVGPSAHIGLCCMLMANSLVAHDTRVGPLCHFSAGSI
                     TGSYNTIGKCSSIAYGTVVLEKKNIGNFAVAGASSLVTKDIPDGEIHIGIPARFMKKV
                     MEA"
     gene            9009..9926
                     /locus_tag="PLI37_05015"
     CDS             9009..9926
                     /locus_tag="PLI37_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOQ58404.1"
                     /translation="MDERIEKLTLMASQLRRDVVNAVYYAKDGHPGPCMSAADLMAVL
                     YFDLLRLNPEDPSWIDRDRFILSKGHACPLWYAALARRGYFPPVELKTLRVLGSRLQG
                     HPDLKKTPGVDANSGSLGHGLSQACGLAIAARKRGSDSLNVVITGDGELNEGIVWEAA
                     MAIAHLKLTNVIAIVDNNGMQSGGKVEKVSGLYPLREKWEAFGWYVVEEDGHDIGRLL
                     PVLERAFQRGRATREERQGMSLLPDHLRRGRASAGVPHPDQPVVLIAHTLKGKGVPYM
                     EGDNSWHKRVPTDEEHQLALSILGGQDLE"
     gene            9919..10887
                     /locus_tag="PLI37_05020"
     CDS             9919..10887
                     /locus_tag="PLI37_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOQ58405.1"
                     /translation="MNNTSSTRVGFMQGLEALAEEDDRIMLVCADSLKAMRADPFLEK
                     FPDRVVELGIAEQNAAGVSAGLALGGLIPYFATYAGFITMRACEQIRTFIAYPNLDVK
                     MVGINGGLAAGEREGVSHQFFEDLAIMRSIPNITVLVPADAGQARQAAKAAVEIPGPV
                     YIRLGSGRDPVIFDDKQPFVPGKIHILEQRGNDLVFFCNGLMIPRVLVASALLEAKGI
                     ACTVVEVHTLKPLDTEAIVELLKDCRAAVTAEDHNIIGGLGSAIMECAAEHCPVPVAR
                     IGLRDVFPGSGHPEALLDFYGMSVNDIVEAAISTINRKPNPLPNEE"
     gene            10889..>11619
                     /locus_tag="PLI37_05025"
     CDS             10889..>11619
                     /locus_tag="PLI37_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOQ58406.1"
                     /translation="MQTGYFKKIKEQTATRLWINNPTIGDVRHGLEAGAINCTTNPTY
                     AARMLREAESRDFALGVIDTVLAACPDDDKAAAIIQRRLVAEMAKGFMPHFEATKGKE
                     GLVSIQMDPRFEEDAAAIVAEALADFALIPNAIAKIPVIPSGLVAIDCLVRLNKPVIA
                     TEIMGIAQALAACEVYKRASEESGFSPAFFVTHISGILDEELKAEAEALGVEVSSEAM
                     YIAGCAVAKKQYRLMKERNLPGILLG"
BASE COUNT         2768 a         2725 c         3149 g         2977 t
ORIGIN      
        1 aagtctttat ttgttggttc ggcaaattta caattttaaa cggttctttt atcggttaag
       61 gcatctttct aaacttacgc ccaacggtgc gggcaacgaa caatttacgc aacaaatata
      121 taacatttgc aattataaga tgtggaggta tttccaaaaa gtgtgcaatt tctatcttat
      181 ttgatgcaaa tgtgtttacg gttatcgata ttacagtaat ggctacattt aactgcgatt
      241 cgtagtgacc ccgttggatc tcaaccacaa ggtctgactc actttcaatt atccggctca
      301 atcccttgac ctgcagaacc ggaatctaaa taactatcca atcttttcac aagttagtct
      361 tcccatcctg acaagacctg cttttagaag atcaatacta tacttgctca tgaacaaccg
      421 accaatcggc tccctgtgtt gtgacaaaac ttgaagagga ggtgtacttg tcttagttgc
      481 atactccaca aacattcctt tctcgaggcg aacacccctg aaggtgccgc tgtccttaac
      541 tgatattctc caaagtggca tactgattaa ttttaaacgt taaacttttt atttattttt
      601 tctttgatgc accctttggt acacttttga cgcatctttg ttacaaagat aaaagccgtc
      661 tctgccaaat cacggcacag accgctagaa aagtgcggaa gacaggacta atgtttacaa
      721 actctttcgt gcgatagaag ttattccata cattatgcat atagcttaaa tagttaataa
      781 gcgctaaaat ctaattattt caatattata atcgaatata tcaatttgct attattatat
      841 ttgtgcagtt ttatacaaat aaccaaataa gacattgcta tgattgagga attcagtttt
      901 gagaattaca aatcgtttaa agagatgaat acgttgaata tgacagctgc aaagctgaag
      961 tcaaagtatt ccaaggtgga tgttgatcca actattcagc tggatgatga tgttacacta
     1021 ttgaagtcga aagccattta cggagcgaac gcatcgggaa agagtaatat tgtgaaagca
     1081 ttcggtagtt ttattcggat tgtacaacat tcagtgaagg atgagcaaat tctggagaag
     1141 cacatggcac ctttttttct ttcaactgaa actttcgaca aacctgtgtt catgcaactc
     1201 gtttttcggt gcgaaggagt tcgttaccgc tatggttttc aagctgacag agagagaatt
     1261 catagtgagt ggctctatgc aacacccggt aaaagagaac agccctattt tatcagagat
     1321 aatgatacct taatcaaaat caataaaaag aacttttcgg aagctgatgt ggtaatgaag
     1381 ttgattgaca atgaaaaagg taaccctatt tttagaaaga actcgttgtt tttgacttca
     1441 ttggctgcat tcggtgtagg taagatatcc aaaatgttgc taaatgagat tgctagattt
     1501 atggttgttg atgggttgaa acacccgggt gcattcgatc agactactgc gtcattagaa
     1561 atgccgggta tgaagaaact tgtacaaacg tttcttgcta tggccgatgc aggtataaat
     1621 gaattgaaaa ccattgaagt acctgatgaa aatgttgggg agagtggcaa tttggcaaaa
     1681 aaaaggaaga ttgtcgttgc tgtgaaagat gtgtatgacg ctaatcataa attattttca
     1741 acgatgccat ttccttttag cttgttcgaa tcggagggaa caaataagat gtatgagatc
     1801 agtacgttca ttatcaatgc actcagagaa ggaagagttt tggtgattga tgagtttgat
     1861 gcccgatttc atccattgat tacccaaaag attgttgagt tgtttaattc tcagggcaat
     1921 aagaatggtc aattgatttt tgtgacgcat gatactaatt tgttgtcggc cgacttgctt
     1981 cgcagagatc agattgactt tgttgagaaa gatacttacg gctccagcca cctatacacc
     2041 ttagtgcaat tcaagggtgt cagagataat gatccctttg aaaaaaacta cataaagggt
     2101 aaatatggtg ccatcccttt tgtcggtgat tttaacaatc tttttgccca ttttgaagat
     2161 gccgaagcgt aaaacatata caaagaaaac ggatattgtg aaggcttgga ataaaaagaa
     2221 aaatccttct caatacgctg ttgacacact tgaaatgaga aaaacctttc tcatcgtttg
     2281 tgagggacaa accgttccat gaacaaaacc ccgttacagc agtttaccag cttgtaagaa
     2341 cgttaaatca gcatattctt ggggaaagag tcctttaact tttttactac atttgtcctg
     2401 gcataattaa tcaatcaatg attccctatc cttactccaa aatctccttg cgcaagtgct
     2461 gcgctttgtt tttgtcggct tgcctggggg cggcctgttt tgctcaaacc acttacgata
     2521 ctattcgaag ttggaatctg gctgtatata gtgcgcagac tctggccgat ctggcggccg
     2581 atacggagcg ctggaatccg atgtataaag acggggtgct gcagcgctat tccaataagt
     2641 tgcctacgga cggcttgccg ctgcaggcca atgggcaggt gattgccgaa gtcgagggcc
     2701 tgctggtggg cagaggcatc gaggcgggca gtttgttgtt gaggcataat atgggaagca
     2761 gccacaacgg gatgcagatg caacggatag ctccggtgag tatttcgggg ctgcgggccg
     2821 gccagcgggt gacggtgacc atacgttctt cgtcgagtgc gcctcagggc atagctgagg
     2881 tgagcaacct cagcggtagt tgcgggccgg gcacctatcc caccacctat ttcaaaacct
     2941 acgccttcga tgtggtggcc gacggagagg cgagctggac aaacagcggg ggcgtggtgg
     3001 tgcagagcgt ggcggtgatg gaggtgagcg aagatatacg cgaacaggtg gctacccccc
     3061 tgatcgaagt ggatggaaag caggtcagcc tgagctgtga aacgccggat gccgtgcttt
     3121 ggtattcgct ggtcgatcat gccgatgtgc tcgattatgc tcgtctttat acggggcctt
     3181 ttgaattgac ccgtccctgc cggttcaggg cgattgctca aaaagaaggg atgcgcaatt
     3241 cactggtggc cgatactaac ctggtggtgg ctgtgacctt tccttttgcc gggcgtcccc
     3301 gggtgctcga tcccgaagcc ctcgacaggg cagcggtggc tacttatatg ggcagcggca
     3361 gccgttacct gatcaactgg cgttggctta tcgacgaccc catgcagatg agtttcaatg
     3421 tgtatcgcga cggggtcagg ctgaacgatt cacccatcag cgaccggaca aattacatgg
     3481 actacgccgg tactgccacc agtgaatata cggtagaggc tgtggtggcg ggcgaggtgg
     3541 tcgaaacggc tcctgtgcgg gtgttgccca aaggctatct cgatattccg ctcaaccgcc
     3601 ccgcgggagg taccaccgag agcggcgatt tttattacat ccccggcgat tgtatggtgg
     3661 ccgatgtgga cggcgacaag gcctacgaag tgattatgaa atgggatcct tccaacggca
     3721 aggacaattc gcagtcaggt tataccggca atgtgctgat cgatgcttat cgcatggacg
     3781 gcactcagct ctggcgtatc gacctgggca aaaacatcag ggcgggcgcg cattataccc
     3841 agttgatggt gtacgatctg gacggcgacg gccgggcgga gctggcctgc aaaacggctc
     3901 cgggtaccat agacggcttg ggcaattatg tgctgatggg cgacgacgat ccccgggctg
     3961 attaccgtac tacggtgggg agcaaaagcg gaatgatcat cagtggcccc gaatacctaa
     4021 cggtgtttag cgggctaacg ggcgaagctc tggctacaac ggcttaccgg cctccgagag
     4081 acaccattag caactggggc gacaactacg gcaatcgcag cgaacgttac ctggcttgcg
     4141 tggcctatct ggatggagag cgtcccagcc tggtgatggt aagaggttat tacacggcgg
     4201 cctttttgtg ggcagtcgat tttgacggcg aaaaactcag cactcgttgg ctgcatagct
     4261 ctattcccgg cggaataggg gcttacggcg aaggggctca cagcatggcc acggccgatg
     4321 tggacggaga cgggcgggac gaaattattt ttggggcctg tgcgctcgac gacgacggaa
     4381 gcattattta ccgtacgggc ctgggacatg gcgacgccct gcatgtgggc gattttatac
     4441 ccgatcgccc cggcctcgag gtgatgatgg tacacgaaga agtgtcggct caatacgggg
     4501 tggaaatgca cgatgccctt accggcgaaa ttatttcggg ccggtttgcc ggcagcgatg
     4561 tgggacgcgg cctttgcgcc gatgtggatc ccacgtcgag aggtcacgaa tattggagca
     4621 cggccagtta cgatgtgtac gacggcgcgg gtacggtgct ttcgaccaaa cgtcctacgg
     4681 tgaatttccg cacctactgg gacggcgatt tgcaatgcga aaccaccgag cgcggagtga
     4741 ttaccaaatg gcagggccgt acgcaaaacg ctgccaccct ggtggatatg cccaataaat
     4801 acggggccgg taccaatgtg attaagtaca caccctgcct gcaagccgat atttttggcg
     4861 actggcgcga agagcagatt tattacgaca acgccacgat gagtcatctc tggatttttg
     4921 ccacgcccta cgttagcaac taccggatac ccaccctgat gcacgaccat cactaccgca
     4981 tggccactgt tacacagacc gccgcctaca accagccccc gcacctgagt ttttacctgc
     5041 ccgattacgt tgagtacctc accgcattgc cgggttcgga ggcaccgtac gatctctatt
     5101 ccgaaggcga tgcctcgcag ggtgccaaac aggtgctgag tactgtctat tacgacctct
     5161 tgggccggcc catcgccgaa cctgagcgtg gcttctacat ccgcgaaaac ctctattccg
     5221 acggcagttt gagtcgcgta aaatgctata agccttaatg taccgggttt aaactgccct
     5281 aagcattaag tgcggtcttt caagatcgct cccggccttt cctgtgaaga aagtttaaac
     5341 tgccttaaac attgagtatg gtacttgaaa taaaaatata taccttcggt ctttcttctt
     5401 aaattttatt actgtgaaaa caaaatggat ttgctgttgt ctttttgcag ccctgctttg
     5461 gccgttcagc gttattacac aggctcagag ggcagaaaca gttcctgcct cccgtaatgt
     5521 tcctgtctcc ggcatggctt cggcttccca catagttcct gcttcccgca tcgctccggc
     5581 ttctctgtct gcccgggctg tgcaagccgt ggttgaaccc gggcagggca ttcaggccgc
     5641 tattgaggcc gcgcccgaac aatcttcggt accttatgtg attttgataa agaagggcac
     5701 ttacaacgaa aaagtgatcg tcgaccgccc caacatagtg ttgctgggcg agcaccgcga
     5761 cagcacccgt ttgatttttg ccgagctgtc gagcaaacgg actattcgcg agtacaaggg
     5821 caagccggtg ggcaacggag tgatagtgct gcaggagggg gccgacgact gcattatagg
     5881 caccatgacg gtttacaaca actacggtag cacggtggag cctaccacgg ctcaccaaat
     5941 ggctattttc ggacgggcta cccgcaccat agtgatcgac tgttgcgtct gggccgacgg
     6001 caacgatgcc ctggcgcttt gggctcccga agggaatggg atgtattacc acgccgattt
     6061 ggatttgcgt tgcccgggag tggattttct ctgtccgagg ggctggtgct acgccacgcg
     6121 ttgcagtttc tacggcgatg gacgggcctt gatctggcac gacggccgtg gcgattacga
     6181 caaaaaattg gtgatcaccg attcgcattt cgactctaaa agccccgtca cgctggggcg
     6241 ttaccatcac gacgctcagt tttttctgct caattgcacc atgagcgaca aaatcatcga
     6301 tcatcccatc ggctacgctt ataccgacca ggtgctggat ccctgtccct ggggacaaag
     6361 gctgtatatg cacaacgtca gacgcgaagg cggtaatttt gcctggatgg ccgacaacct
     6421 ggatcaggcc aaaggctcgc cccgccccga agacatcacg gccctctgga ccttcggcgg
     6481 cgcctgggat cccgaacaaa agatagcgga gctgaaggaa ctgctgaaga caaacagata
     6541 gaaaattcct acatttgcac ccatgattca caagcttgcc gatcgtctgc ggggcaggaa
     6601 taaaccggcc aaggagggtt accgtacggc cttcaaggct acgagcctgt ttggaggggt
     6661 gcaggtagtg actgttttga caggtctgct taagtccaag ctggtggctg tctggatggg
     6721 cactacgggt tttggcatac ttagcttgta taacgcggcg gtcaacctga tcttttcgat
     6781 cagtaatctg ggcttgcaga gttctgcagt gcgcgatatc gcggctgctt cggccgacaa
     6841 gggaaaaacc ctcgccatca tcaaagccgt caatcgctgg gtattggcca gcggcctgct
     6901 gggtgccttt cttaccgttg ccctttcgcc ccggctcagt cgttggttgt ttgagtccga
     6961 ccaatacgtg ggggcttttg ttttgctctc ggcagccgtg ttgctgatgg gcctgttcaa
     7021 tcagaattac gcggtattgc agggtctgag gaagctcagg ctgatggcgc gggctaatat
     7081 tttcggcgcc ctggcgggct ttgtctgctc ggtgccggtt ttttattttt tccgcgaaca
     7141 gggtttggcc tggacggtgg tgatttcggc cctggcctct gcggtggtga gtttgttcta
     7201 cgttcgtaag ctgggcctgg ttttacccaa acaagattgg catacctcga tggtgttggg
     7261 cagcgatacc gttaaactag gtatcgtgat ggccatcagc agtatagctg tatttatggt
     7321 gcaatttgta gttaagacct atatttcgca ccggggcggc ctgggcgatg tgggccttta
     7381 tcaggctggt tggggcttga acgagcaata tttgggcctg gtgtttacgg ccatggccaa
     7441 ggattatttt ccccggctgt cgcaggtggc cggcgaccac cggaaagttc gtaaaagcat
     7501 caatgagcag gccgagattg ctattctgat tttggcgccc atgataatag cgatgctggt
     7561 gtttctgcct tttgttgtgc gtctgctcta ttcgggcgat ttcgacggca tagtacctat
     7621 gacccgctgg ctcttgctgg gctctttgct caaggccggt tcctgggcta tttcctttgt
     7681 ctttctggcc aaaggacacg gcaaaaccta tttattcaat gaattgggca tcaaattctt
     7741 cactttgccc acttacatgc tggggtatag attgttcgga ctcctgggcg taggctatgc
     7801 ctatgtgctt tgttacggca tttatttcct ttgggtgggc atagtggcat accggaaata
     7861 tcgtttgatg tattccgggg tattttggag aatgctggca tttaccacca cggtgctggc
     7921 agcttatctg ctgatggaac ccctgatgat ttccggaggc ttgcattatt ggctgggtat
     7981 tccgctgatt gttttactga gcatctattc gctgcgggaa ttgaaaaaac gattgtttta
     8041 gtcgaatgga ttataacatg cagagaacat tttgatctac agaggacatt ttgattcagg
     8101 tgattattcc aaaggactcg gcggaaagtt tttttgtaga accggtggaa acaatacttt
     8161 tgcaagaaga aagaatgaga aaccataaga acgattagga tatgagtaaa aaaatactga
     8221 ttatcggcgg cgaaggcaac ggcggtgtgg ttgctgcctg tatagaagat atgcgtctgc
     8281 gccatcgcat cctcgaatgg gaggtggccg gttttatcaa cgactacgaa caaagcgtca
     8341 gcggctatcc ggtgttggga ggcaccggcg acgtgcagga tattctgcga agacaccccg
     8401 attattatct gatgtgggct attcacacca taggacgtaa ttacttgatc gagaaggtgt
     8461 ttaacaaggc gaatatcccc gcggaacgcc tggcgaccat agtgcatcct tcggcttttg
     8521 tggctcctac tgcccatttg gagccggggg tgtttgttat gtccaattgc tacgtcggcc
     8581 cgtccgctca tatcggcctc tgctgtatgt tgatggccaa ctccctggtg gctcacgata
     8641 cccgggtggg gcccttatgt catttttcgg ccggttccat taccggctcc tacaacacca
     8701 taggtaagtg ttcctccata gcctatggta cggtggtgct cgaaaagaaa aacataggca
     8761 attttgcggt agccggggct tccagcctgg taaccaaaga tattccagac ggcgagatcc
     8821 atatcggaat tccggcccgt tttatgaaaa aagtgatgga agcctaatcg gccgtaattt
     8881 ttaagggttc ggcctttttg ttgtgatttt ttaccgaaat agaaggcccg aaagtttttg
     8941 tcgagaacgg agaatgccct atatttgggt tctgaatgct cttttgagca aaaagattta
     9001 acatagttat ggatgaacgc atagaaaaac tgacccttat ggcgtcgcag ttgcgccgtg
     9061 acgtggtcaa tgcggtatat tacgctaaag acggccatcc cgggccctgt atgtcggctg
     9121 ccgatttgat ggccgttttg tattttgatt tattgcggct taatcccgaa gatccttcct
     9181 ggatagatcg cgaccgtttt atcttgtcca aggggcatgc ctgtcctttg tggtatgccg
     9241 ctttggctcg cagaggctat ttccctcccg tggagctcaa aaccctcagg gtgctgggtt
     9301 ccaggttgca aggtcatccg gatttgaaaa agactccggg ggtagacgcc aattccggtt
     9361 cgctgggaca cggcctgtcg caagcttgcg gcctggcgat tgcagcccgt aaacggggtt
     9421 cggatagcct gaatgtggtg attaccggcg acggagaact caacgaaggc atagtatggg
     9481 aagcggctat ggccattgcc catctaaagc tgaccaacgt cattgccata gtcgacaaca
     9541 acggcatgca gagcggaggt aaggtagaaa aggtgagtgg cctgtatccc ctgcgcgaaa
     9601 aatgggaagc ctttggctgg tatgtggttg aagaagacgg acacgatatc ggccggcttt
     9661 tgcccgtgct cgaaagagcc tttcaacggg gcagggcaac gcgcgaagaa cgtcagggaa
     9721 tgagcctgct gcccgatcac ctcaggcggg gacgggcttc ggcgggcgtg ccgcatcccg
     9781 atcagcccgt ggtgctgatt gcacatactc taaagggaaa gggtgttccc tatatggaag
     9841 gcgataattc ctggcataag cgtgtgccta ccgacgaaga acatcaattg gctttatcaa
     9901 ttttgggagg acaagatctt gaataataca tcgagtaccc gtgtgggttt tatgcagggc
     9961 ctggaggctc tggcagaaga agacgacaga ataatgctgg tttgtgccga cagcctcaag
    10021 gctatgcgtg cagacccttt tctggagaaa tttcccgaca gggtggtgga gctgggcata
    10081 gcggagcaaa atgcggccgg tgtatcggcc ggtctggcct tgggcgggtt gattccctat
    10141 tttgccactt atgcgggctt tatcaccatg cgggcctgtg agcagatccg tacttttatt
    10201 gcctatccca acctggatgt gaagatggtg gggatcaacg gcggtctggc ggccggcgaa
    10261 cgcgaagggg tgagccatca gttttttgaa gatttggcca ttatgcgcag cattcccaat
    10321 ataaccgttc tggtgccggc cgatgccggt caggcccgtc aggcggcaaa ggcggcagtc
    10381 gaaattcccg ggccggtgta catccgtctg ggcagcggtc gcgatccggt gatttttgac
    10441 gataaacagc cctttgtgcc gggcaagatc cacatcctgg aacaaagagg caacgatttg
    10501 gtcttttttt gcaacggcct gatgattccc cgggtgctgg tagcttcggc tttattggaa
    10561 gccaaaggca tagcctgtac cgtggtcgaa gtgcataccc ttaaacccct cgatacggag
    10621 gctatagttg aacttttgaa agattgccgg gcagcggtga cggccgaaga tcacaacata
    10681 ataggcggtc tgggttcggc catcatggaa tgtgctgccg agcattgtcc ggtgcctgtg
    10741 gcccgcatcg gtttgcgcga tgtctttccg ggttcgggtc atcccgaggc cctgctcgat
    10801 ttttacggca tgagtgtgaa cgatatagtg gaggccgcca tcagcaccat caacagaaaa
    10861 cccaatcctt taccaaacga agaataatat gcaaacaggt tattttaaga aaataaaaga
    10921 acaaacagct acccggctgt ggatcaataa tccgaccatc ggggatgtgc gccatgggct
    10981 ggaagccgga gccatcaatt gcaccaccaa tcccacttat gctgctcgta tgctgcgcga
    11041 agccgaaagc cgggattttg ccctgggggt gatagacacc gtgttggccg cttgtcccga
    11101 cgacgataaa gccgctgcga ttatccaacg ccgcctggtc gcggagatgg ccaaaggctt
    11161 tatgccgcat ttcgaagcca ccaaaggcaa agaaggtttg gtgtccatac aaatggatcc
    11221 ccgtttcgaa gaagatgctg cggctattgt ggccgaagcc cttgccgatt ttgctcttat
    11281 tcccaacgct atagccaaaa ttcccgtgat tccttcgggt cttgtggcca tcgattgcct
    11341 ggtcaggctg aacaaaccgg tgatcgccac cgaaattatg ggcatagctc aggcactggc
    11401 tgcctgcgag gtatataaac gggcttccga agaatcgggt ttttctcccg ccttttttgt
    11461 gacccatatt tcgggcatcc tcgacgagga gctcaaagcc gaggccgaag ccctaggcgt
    11521 ggaagtcagt agcgaagcga tgtacattgc cggctgtgcc gtagccaaaa aacaataccg
    11581 cctgatgaag gagcgaaact taccgggtat tttactcgg
//
LOCUS       DAOSTD010000056        11607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_5510_length_11607_cov_15.535232, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000056 DAOSTD010000000
VERSION     DAOSTD010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11607
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5510_length_11607_cov_15.535232"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLI37_05030"
     CDS             complement(<1..221)
                     /locus_tag="PLI37_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOQ58407.1"
                     /translation="MREIEQLIEISRFYGKDKRFVIAGGGNTSYKDDRQIWVKASGFA
                     LATIDRDGFAVLDRDKLRLLSEKSYSDDT"
     gene            complement(285..1496)
                     /locus_tag="PLI37_05035"
     CDS             complement(285..1496)
                     /locus_tag="PLI37_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58408.1"
                     /translation="MKIVNVNAHLHTPYSFSAFESLTQALDLALEQGVRVVGINDFYS
                     MDGYELWEKECRKRSLYPLFNIEFIGLNQQDQAQGIRVNDPNNPGRTYISGKGLKCPP
                     SLPEPYASQLSLVRAESNAQVKAMCAKINDLLDSLKLGFRLDYEQILREMTKGNIRER
                     HLAKALRIKVYEQALGPVSGSKKQISEAGREAEAKIAALFQQLFGGKALKSAINDHAA
                     VENEIRGNLLKAGGAAFVPEDPKAFLPVDKVCSIILAAGGIPTYPFLADDPKGGYTDF
                     EGNLENAAKTLHERGFHSVEFISTRNDLHLLEKYVVYLYELGFVVTLGSEHNSPAMEP
                     ILLSARHGQALSEVLREINYKSACVLAAHQHLVGQGLEGYVDASGRAHTDKREEYIKL
                     GDQLIRSVTGQ"
     gene            complement(1590..>2132)
                     /locus_tag="PLI37_05040"
     CDS             complement(1590..>2132)
                     /locus_tag="PLI37_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOQ58409.1"
                     /translation="QRIYDIGEQFHQGGVPEGYYEIPLGEPDIKRPGKDITILTIGAT
                     LYRALEAADILQEQYGISAEVIDARSIVPFNYDLVIESVKKTGRILLSSDASSRGSII
                     NDMARNISELAFDYLDAPPVVVGSRNWITPAHELEDAFFPQASWMLDAIHEKIQPLKN
                     YIPKQNFSNSQQILRNKQGV"
     assembly_gap    2133..2232
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2233..3566)
                     /locus_tag="PLI37_05045"
     CDS             complement(<2233..3566)
                     /locus_tag="PLI37_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOQ58410.1"
                     /translation="MPKQLFIDPSEMRKAGEVTFKPIPVNQYNKTAKQELDEGNFSKQ
                     DLLDIYHDMSLIREFETMLHLVKTTGAYKDIPYNNPGPAHLSVGQEAAAVGMAYTLDV
                     DDFIFGSHRSHGEILAKGMSAIRKLDEDSLKKIMQEFLGGATLKVAGMNFEGNTRQLA
                     RRFLVYGALAEIFARVTGFNRGLGGSMHAFFTPFGIYPNNAIVGGSGDIAVGAALYKK
                     INRKPGLVVANLGDASMACGPVWEGLVFSAMDQFKTLWDDDMKGGLPFILNIMNNQYG
                     MGGQTQGETMGYDIAARIGAGINPEQMHAERVDGYNPLAVIDAYRRKRQVINDKQGPV
                     LLDVLTYRYSGHSPSDASSYRSKEELEAWEAQDCIMGYAKQLLEGNICKQADLDRIQE
                     DIRETILEVYKLAIDLDISPRMDLDQDQELLGEAMFSNQSVDSMMPGVKPEV"
     gene            complement(3593..>4417)
                     /locus_tag="PLI37_05050"
     CDS             complement(3593..>4417)
                     /locus_tag="PLI37_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ58411.1"
                     /translation="FFNSLGSKVTVVEMLPEILGGLDLEISAMLRNIYAKKGIDFHLQ
                     AKVVKVQGNEVFFEKDGQTQSVKGDKILLSVGRRAVTQGYGLENLGVELHRGGIKVDE
                     LMRTNIPGVFAAGDVTGFSLLAHTASREGEVVVNNILAARQAAQSGSKVRYDRMRYNA
                     IPGVVYTNPEVASAGLSEEAARAANIEFKAIKLPMAYAGRFVAENEGGNGLCKIIVDS
                     RNGRVLGVHMLGNPCSEIIYGACMAIEQEMTLEELKQVVFPHPTVSEIIKEVAYNV"
     assembly_gap    4418..4517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4518..4818)
                     /locus_tag="PLI37_05055"
     CDS             complement(<4518..4818)
                     /locus_tag="PLI37_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ58412.1"
                     /translation="MMYDLAIIGGGPAGYVAAERAGSQGLSVLLFEKASLGGVCLNEG
                     CIPTKTLLYSAKTYENALQGDKYGITATGVEFDFGKMMARKNKVVRKLVAGVEAQM"
     gene            complement(4899..6323)
                     /locus_tag="PLI37_05060"
     CDS             complement(4899..6323)
                     /locus_tag="PLI37_05060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOQ58413.1"
                     /translation="MAVVIIMPKQGQSVESCIISSIKKKGDKVAKGDILFAYETDKAS
                     FEEEAPRDGVVLECWYNEGDEVPVLQNVMILGDAGEDYSALLAEMDAGAQGSGQTAPP
                     ASAASTTEVPAAGVTAPAPAAQASAPASSDQPGAVASQADAASTSSVIASGGTVFISP
                     RARKLAEKEALDFRQLSGSGPGGRIIESDVQAALQNRPKLSPLAKKTLQEQAAVVPEQ
                     GSGLAGTVLASDLKAPVNAVYGIDYQDKKLSNMRKLIAKSMYASLQNSAQLTHHLGAD
                     ARRIMELRKQVKEAMDQGKLNVNITLNDMVCLAVIKALKKFPNVNSHFLGDSMRLFNK
                     VHLGLAVDTERGLMVPALRNADDLSISGLASQLKELAAACRKGAIDPDLLSPEAASFT
                     VSNLGNYGVEMFTPVINLPQSAILGVNTIVPRPKDLGGGVYAFVPYIGLSLTYDHRAL
                     DGGEATRFLKQIAVEIENLEFDIA"
     gene            6573..7316
                     /locus_tag="PLI37_05065"
     CDS             6573..7316
                     /locus_tag="PLI37_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOQ58414.1"
                     /translation="MFQEIMYICAMFEESGLPLYKSLYEKLRRQIEEGIYRSGDLLPS
                     ENELSALYGLARSTVRNALDRLVNDGFIVKQQGRGSIVKGKAKGVGILSLKSTSSALG
                     GNLSTRLIVKPEIRSWKEAFGYTISQNERDAGCIYFERLRLINNRPVFFDITMLPNIN
                     LPRFTMHKLENASLFDLLRTAYQIEVTGGVQHLYALKANKELQEYFQVKAGHPVLQLN
                     RKIETNRPGMHIYSKVFCNTQEYALFGTF"
     gene            7320..7763
                     /locus_tag="PLI37_05070"
     CDS             7320..7763
                     /locus_tag="PLI37_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOQ58415.1"
                     /translation="MDFFTGLGLFGLFLGSFLAGSIVPFSSEVLLGGALLAGVDPVAC
                     LISTSLGNTLGGMSCYYIGRLGKMEWMTKYLKVKETTLHKYTTFLRGKGAWLGFFAWL
                     PYAGEAISVALGYLRANALIVAVSMLIGKTLRYLVMMLIILGVIA"
     assembly_gap    7774..7873
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8133..8771
                     /locus_tag="PLI37_05075"
     CDS             8133..8771
                     /locus_tag="PLI37_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ58416.1"
                     /translation="MADLLREDILKTSTKLFFKFGLRSVSIEDICNELRISKKTFYTC
                     FSQKEALIEDILIDYDEAFAKRMELKIKSLNLPESTTVIDMVLFFSIFHLINRDARFE
                     NFFFDMQKYYPEVFKRHMDRHRHIVTNHIRDMLLKGIEQGLYRAELETELTAKIITLE
                     LISMMEFARNKLNKSEQQKVIMLLTDLHMRSLCNERGLKYYLKALDKLENQQ"
     gene            8858..10180
                     /locus_tag="PLI37_05080"
     CDS             8858..10180
                     /locus_tag="PLI37_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOQ58417.1"
                     /translation="MKTIIPSNRVKLFTLCLSGLLSLSSLPFTSRAEETPQQWRFSLE
                     EAVGHALVHNTAMIKADLAVRQAEAAKWAAIANYLPQLNVNINYNNYLGASIEIFGMD
                     IPMDPSSRLTLQASQLIFNANAILGMQLSELSRRMSELGSNQSELAVKQNVRSTYYSI
                     LMMENNRLLLKKNVENMRNLAKATRAKVEVGIGEQTEADEIEIVLANLENTLKQTERT
                     IELTYNSMRLLLGLNAEDGIELSDRLQDVTDKQAMFALLAAGFDPAENVDMKAGQLNL
                     EINKKQVKSAIAGYLPTISAVYQRNEILLKTGFDPTTKNTLVLTASMPLFCSGKTTAE
                     LKKAQLAYESAQEDLELMQKQLLIQDKQLRFNLKSALESYEIQERNIEVSKRMFDNVT
                     KKFEQGLSSSLEVTNASNNLLTAQSNYIQSAMSLLNASDALKKLLGTL"
     gene            complement(10186..10323)
                     /locus_tag="PLI37_05085"
     CDS             complement(10186..10323)
                     /locus_tag="PLI37_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58418.1"
                     /translation="MFYFLFLFPVVVADDVFVVVVAAVVAVVVAVVVAVVVAATDDND
                     D"
     gene            10339..11373
                     /locus_tag="PLI37_05090"
     CDS             10339..11373
                     /locus_tag="PLI37_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOQ58419.1"
                     /translation="MKNPLSALPALLIIATGLYACSGASKSREAQLETTQEKIENVKV
                     TTLSSRMVGNTLEFPANTRAYERVNLGPVSPGRIEKIYVEMGDRVKKGQLLIQMDQTA
                     LLQARIQIANFEEDHRRYEALKESGAISQQVYDQSLANLNMQKTNLDYLEKNTRIKAP
                     FDGIIALRNFENGELYPGGSAILTLMEIDKLKADINVPESYYPLIKKGMKAEVFCDTY
                     PGRQFEAEVNLIAPTVNPASRSFAVEIKIPNTKEWLKPGMFVRIHIDLGVSEAILVPS
                     QAVLKLQGSNERFVFLHRNGLAQRVPVQIGRRFDDEVELISDRIVAGDQLVVLGQARL
                     RDGVKLNVIE"
     gene            11384..>11607
                     /locus_tag="PLI37_05095"
     CDS             11384..>11607
                     /locus_tag="PLI37_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOQ58420.1"
                     /translation="MSIYKSTVNKPVTTLMVFAAVMVAGLYALYHLPIDQFPEIEPPF
                     VTVMTTYSGANASEIETNITELLEDAFNSV"
BASE COUNT         2855 a         2726 c         2844 g         2882 t
ORIGIN      
        1 tcggtatcgt cgctgtagga cttttcggag aggagccgca gtttgtcgcg atcgagtacg
       61 gcaaaaccat cccgatcgat ggtggccagg gcaaagccgg aggctttcac ccaaatttgc
      121 cggtcgtctt tataagaggt gttgcctccg cctgcaataa caaaacgctt gtctttaccg
      181 taaaaccggg atatctcgat gagttgttcg atctctctca tgacattatt gtttaattga
      241 ttagaaaatg aaaagtccat acttgtgttc ttatttattc ttgtttattg gccggtgacg
      301 gaacggatga gttggtcgcc cagcttgatg tattcttcgc gtttatctgt gtgagcacgg
      361 cctgaagcgt ccacgtagcc ttcgaggccc tgaccgacca ggtgttgatg ggctgccagc
      421 acgcaagcgc ttttgtaatt gatttcacgc aaaacttcgc tcaatgcttg tccgtgacgg
      481 gcagacaaca aaatgggctc catggcgggg ctgttgtgct cgctgcccaa agtgacgaca
      541 aaaccgagtt cgtagagata aacgacgtat ttttccaata gatgcaggtc gttgcgggta
      601 ctgatgaatt ccaccgaatg aaaaccccgt tcatgcaggg tcttggctgc attttccaga
      661 ttgccttcga agtcggtata gcctcctttg ggatcgtcgg ccaaaaaagg ataagtgggt
      721 atgccgccgg cagccaatat tatgctacag actttgtcca caggcaaaaa ggctttggga
      781 tcttcgggta caaaagcggc gccgccggcc ttgagcaaat tgccccggat ttcgttttcg
      841 acggcagcat ggtcgtttat ggcagacttg agggctttgc ctccgaacag ttgctgaaaa
      901 agggctgcaa tttttgcttc ggcttcccgg ccggcctccg aaatctgctt tttgcttccg
      961 gaaacgggac ctaaggcttg ttcgtagacc ttaatgcgca aagccttggc caaatgccgt
     1021 tcgcggatgt tgcccttggt catttctctg aggatctgct cgtaatcgag gcggaaaccc
     1081 agcttcagac tatcgagcaa gtcgttgatt ttggcgcaca tggccttgac ctgggcattg
     1141 gattcggctc tgacaagact gagctgagat gcgtaaggtt cgggcaacga gggcggacat
     1201 ttaagaccct tgccgctgat ataggtacgg ccgggattgt ttggatcgtt tacccgtatg
     1261 ccttgggcct gatcctgctg gttgaggccg ataaattcta tattgaacaa aggataaagc
     1321 gagcgcttgc ggcattcctt ttcccagagt tcgtagccgt ccatggaata aaagtcgttg
     1381 attccaacca ccctgacccc ctgctccaac gccagatcga gagcctgtgt aaggctctcg
     1441 aaggcgctga acgaataagg cgtatgcaaa tgagcattaa cgttcactat tttcattgtt
     1501 tttattttta ttcagtgatt ccgtattgga taaatggttt tttaattatc aatgaaatat
     1561 taaaaaaacg gattcaaacg gcaaagatac taaactccct gtttattcct aagaatctgt
     1621 tgagaatttg aaaaattttg tttgggaatg taatttttca agggttgaat tttctcatga
     1681 atggcatcca gcatccaact tgcctggggg aagaaagcat cttccaattc atgggcgggt
     1741 gtgatccagt tgcgtgaacc cactaccacg ggaggagcgt ccaggtaatc gaaagccagt
     1801 tcgcttatgt tgcgggccat atcgttgata atggatccgc ggctgctggc gtcgctcgaa
     1861 aggagaatac gtccggtttt tttgaccgac tcaattacca gatcgtaatt gaagggcact
     1921 atgctgcggg catcgatcac ttcggcgctg atgccgtatt gttcctgcaa tatgtcggca
     1981 gcttcgagag cccgatagag ggtagcccct atggtgagga tggttatgtc cttaccggga
     2041 cgtttgatgt cgggttcgcc caggggaatt tcgtaatagc cttcgggcac tccgccttgg
     2101 tggaattgtt caccaatatc gtagatgcgc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nntttacttc gggcttcact ccgggcatca tgctgtcgac cgattggttg
     2281 gaaaacatag cctcgcccag caattcctga tcttggtcca aatccatacg gggagaaatg
     2341 tcaaggtcga tggccaactt ataaacttcc aaaatcgtct cgcggatgtc ttcctgtatc
     2401 cggtcgagat cggcttgctt gcagatgttg ccttcgagca attgcttggc gtagcccata
     2461 atgcaatcct gggcttccca ggcttcgagt tcttctttgc tgcgatagga cgaagcgtcc
     2521 gagggggaat ggccgctgta gcgataggta agtacatcga gaaggacagg cccctgtttg
     2581 tcgttgatca cctgacgttt acggcgatag gcatcgatca ctgccagggg attgtagccg
     2641 tctactcttt cggcatgcat ttgttcgggg ttgataccgg ctccgatacg ggctgcaatg
     2701 tcgtaaccca tggtttcgcc ctgggtttgt ccgcccatac cgtactggtt gttcatgatg
     2761 ttgaggataa aaggcaggcc gcctttcata tcgtcgtccc aaagagtttt aaactgatcc
     2821 atggcagaaa aaaccaaacc ttcccaaacg ggaccgcagg ccatggaggc gtcgcccagg
     2881 ttggcgacca ccaagccggg tttacggttg atttttttat agagggctgc tcccacggca
     2941 atatcgccgc taccgcctac gatggcgttg ttgggataaa tgccgaaggg ggtgaaaaag
     3001 gcgtgcatac tgccgcccag gccccgattg aaaccggtta cgcgggcaaa gatttcggcc
     3061 agagcgccgt agaccaaaaa gcgacgggcc agttgccggg tgttgccttc gaagttcatg
     3121 ccggccacct taagagtggc tccgccaagg aattcctgca tgattttttt aaggctgtct
     3181 tcgtcgagtt tacgaatagc cgacatgccc ttggccagga tttcgccgtg gctgcggtgc
     3241 gagccgaaaa taaagtcgtc cacgtcgagg gtataggcca taccgacggc ggcggcttcc
     3301 tgtcccaccg agaggtgagc gggaccgggg ttattgtagg gtatgtcttt gtatgctccg
     3361 gtagttttga ccagatggag catggtttcg aattccctga tcagggacat gtcgtggtaa
     3421 atgtcgagca aatcctgttt gctgaaattg ccctcgtcca gttcctgttt ggctgtttta
     3481 ttgtactggt ttacgggaat gggcttgaat gtaacttccc cggctttacg catttccgag
     3541 gggtcgatga ataattgttt aggcatgatg aaaatatttt atgtgttttg ttttatacgt
     3601 tgtatgctac ttctttgata atttcgctga cggtgggatg aggaaagacc acttgtttga
     3661 gttcttcgag ggtcatttcc tgctcaatgg ccatgcaagc tccataaatg atttcgctgc
     3721 agggattgcc cagcatgtgc acgcccagca ctcggccgtt ccggctatcg acgatgattt
     3781 tgcagagacc gttgcctcct tcgttttcgg ccacaaaacg cccggcatag gccatgggga
     3841 gtttgattgc cttaaactcg atattggcag cccgggcggc ttcctcgctg aggccggcgc
     3901 tggctacttc ggggttggtg taaaccacac ccggaatggc gttgtagcgc atacggtcgt
     3961 aacgaacctt gcttcccgat tgtgcggcct gacgggcagc gaggatattg ttgaccacta
     4021 cttctccttc gcgcgaggcg gtatgagcca acaggctgaa accggtgaca tcgcccgcag
     4081 caaaaacgcc cggaatattg gtgcgcatga gttcgtccac cttgatgccg ccgcgatgaa
     4141 gttctacacc caggttttcg aggccgtatc cctgggtaac ggcgcgacgg cctacactga
     4201 gcaaaatctt atcgcccttg accgattggg tctgaccgtc tttttcgaag aaaacttcgt
     4261 tgccttgcac tttgactact ttggcctgca aatggaagtc gatgcctttt ttggcgtaaa
     4321 tgttgcgcaa catggccgag atctccaggt cgagaccgcc caaaatctcg ggcagcattt
     4381 ctacgacagt gactttactg cccaggctgt tgaagaannn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnntca tttgagcttc gacgccggcc accaatttgc gaacgacttt
     4561 gtttttgcgg gccatcattt tgccaaagtc gaattcgact ccggtggctg taataccata
     4621 cttatcgccc tgaagtgcat tttcgtatgt cttggcgctg tagagcaaag ttttggtggg
     4681 aatgcagcct tcgttcaggc aaacaccgcc cagactggct ttttcgaaaa gcaacaccga
     4741 caagccctga ctcccggctc tttcggcggc tacgtatccg gcggggcctc cgccaataat
     4801 cgctaaatcg tacataatgg ttggaattta tttatttcga ttgtttttaa ttttcttctt
     4861 gggatttatg ggatttaatt accgacaagc ccgggtctct aggcaatatc aaattcgaga
     4921 ttttcgattt cgacggcgat ctgcttcaga aagcgggtgg cttcgcctcc gtccagtgcc
     4981 cgatggtcgt aggtgaggct caggccgata tagggcacga aggcatacac gccgccaccc
     5041 aggtctttgg gacggggcac tatggtattt acgcccagga tggccgattg cggcaggttg
     5101 atgacgggag taaacatttc cactccgtaa ttgcccaggt tggatacggt aaagctggcc
     5161 gcttcggggc taagcaggtc ggggtcgatg gctcccttgc ggcaggcggc ggccaattcc
     5221 ttaagttgcg aggccaggcc gctgatgctc agatcgtcgg cgttgcgcag ggcgggtacc
     5281 atcaatccgc gttcggtatc gacggcaaga cccagatgca ccttgttaaa cagacgcata
     5341 ctgtcgccta agaaatggga attgacattg gggaattttt taagggcctt gataacggcc
     5401 aaacacacca tatcgttgag ggtgatgttg acgttgagtt tgccctggtc catggcttcc
     5461 ttgacctgct tgcgtaattc cataatacgg cgggcgtcgg cccccaggtg gtgggtaagt
     5521 tgagctgaat tctgcaaaga ggcatacatg gatttggcaa taagtttacg catattgctc
     5581 agtttcttgt cctgatagtc gatgccgtaa acagcattga ccggagcctt gaggtcggaa
     5641 gccagtacgg tgccggccag gccgctgccc tgttcgggca caacggctgc ctgttcttgc
     5701 agcgtttttt tggccagagg gctcagcttg ggacggtttt gcaaagctgc ctgcacatcg
     5761 ctttcgatga tacggccgcc ggggccgctg ccgctgagct gacggaaatc gagggcttct
     5821 ttttcggcca gtttacgggc acggggtgaa ataaaaacgg taccaccgga ggcgatgacg
     5881 gaacttgttg aggctgcatc tgcttgcgaa gcaactgcgc cgggctgatc cgagcttgct
     5941 ggggcggaag cctgcgctgc cggagcgggg gctgttacac ctgcggccgg tacttcagtt
     6001 gtagaagcgg ccgaagcagg aggtgctgtt tggcccgaac cctgagcgcc agcatccatt
     6061 tcagccagca gagccgaata gtcttcgccg gcatcaccta aaatcatgac gttttgcaac
     6121 accggaactt cgtcgccttc gttgtaccaa cattccagca caactccgtc ccggggggct
     6181 tcttcttcga acgaggcctt gtcggtttcg taggcaaata ggatatcgcc tttggctact
     6241 ttgtctcctt ttttcttaat ggaagagatg atgcaacttt ccacggattg cccttgtttg
     6301 ggcataataa ttactactgc catagctttg atttatataa tatttgtttg tttactcagg
     6361 tttcgaacag caaaggtaaa gttataacta accgataaaa atattctttc tttcaacgac
     6421 tatcggtttc ggacagcaaa ggtaaaatta ttacttgtat atacaagttt tttaggtgta
     6481 atattttatt aaaaatacaa cattcttttt atcaaacact taaaagatta caagtgccga
     6541 aacttgtatg tacaattttt attatttgtt gcttgtttca agaaataatg tatatttgcg
     6601 ccatgtttga agaaagcggt ttaccacttt acaagtcttt gtacgaaaag ctgcgccgcc
     6661 agatcgaaga aggtatttac cgttcgggcg atctgttgcc ctcggaaaac gagttgagcg
     6721 ccttgtacgg actggccaga tcgacggtgc gcaacgccct ggaccgtctg gtcaacgacg
     6781 gttttattgt aaagcaacag ggacggggca gcattgtaaa aggcaaggcc aaaggagtgg
     6841 gcattttgtc gctcaaaagc acctcttcgg ctttgggcgg caatctgagc acccggctta
     6901 ttgtcaagcc cgaaatccgt tcctggaaag aggctttcgg ttacaccatc agccagaacg
     6961 aacgcgatgc cggctgcatc tattttgagc gcctgcgcct gataaacaac cggccggttt
     7021 tctttgacat caccatgctg cccaatatca acctgccccg ttttaccatg cacaagctcg
     7081 aaaacgcttc cttgttcgat ttgttgcgca ccgcatacca aatcgaagtt accggaggcg
     7141 tgcaacatct ttatgccctc aaggccaaca aggaactgca agaatatttt caggtcaaag
     7201 ccgggcatcc ggtcttgcaa ttgaaccgaa agattgagac taaccggccg ggtatgcaca
     7261 tatacagcaa agttttttgt aacacacaag agtacgcact atttggaacc ttttgagcta
     7321 tggatttttt tacaggctta ggacttttcg gcctttttct cggctcgttt ttggccggca
     7381 gtatcgtgcc ttttagttcc gaagtgttgt tgggaggtgc tttgctggcc ggagtcgatc
     7441 ctgtagcctg cctgatcagt accagcctgg gcaacacctt ggggggaatg agctgctatt
     7501 atatcgggcg tttgggcaaa atggaatgga tgaccaaata cctgaaagta aaagaaacca
     7561 ctctgcataa atataccacc tttttgcgcg gtaaaggcgc atggctgggc ttttttgcct
     7621 ggttgcccta cgccggcgaa gccatctccg ttgccctggg ttatttacgg gccaatgcct
     7681 tgattgttgc agtatccatg ctcattggca aaaccctgcg ctacctggta atgatgctga
     7741 tcattctggg ggtgattgcc tgatatcccg agtnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnacactcc ggtgctgatt acacggcatt tcggtgcttg cccgattttt
     7921 acttgttttg gttggaaata catagaaaca ggaaacttta gtgttttctc agtttccatt
     7981 atgtatctgt ttatcaactt gttataaaat attgtttatt gtttaaacat atttaacgca
     8041 ccgggatatc ggaaaatcgg ttttgatttt acctttgccg cgttttttag gttaataaca
     8101 aacttacttc acgacgacaa acttacttca caatggcaga tttacttcgc gaagatatac
     8161 ttaaaacctc gaccaagctg ttttttaaat tcggcctgcg cagtgtatcc atcgaggata
     8221 tctgcaacga actgcgcatt tctaaaaaaa ccttctacac ttgcttttcg caaaaagagg
     8281 ctctgatcga agatatactg atcgattacg acgaggcttt cgccaaacgt atggaattga
     8341 aaatcaaaag cctcaatctg ccggaatcga ctacagtgat agacatggtg ctcttctttt
     8401 cgatttttca tctgataaac cgcgacgccc ggttcgaaaa cttctttttt gatatgcaaa
     8461 agtattatcc tgaggtattt aaacgccata tggatcgtca ccggcatata gtgacaaatc
     8521 acatccgcga tatgctgctc aaaggaattg agcagggact ctatcgtgcc gaattagaga
     8581 cggagcttac cgccaaaata attactctcg agttgatttc catgatggaa ttcgccagaa
     8641 acaaactgaa taaaagcgaa caacaaaaag tgataatgct gctcaccgac ctgcatatga
     8701 ggagtttatg caacgagcgt ggcctgaagt attacctcaa ggccctcgat aaactcgaaa
     8761 atcaacagtg aatcggtggt acaaagccgt aaccaatcga cagggatttt aaaaaccaag
     8821 aatcaaaaaa caaaaacaga tatagaatta aatagcaatg aaaactatta ttccatcaaa
     8881 tagagtaaaa ctgttcacgc tctgcctgtc ggggctttta agcctgagca gcctgccgtt
     8941 tacaagccgg gcggaagaaa cacctcaaca atggcgtttt tcgctcgaag aagcagtcgg
     9001 ccatgccctg gtgcacaaca cggccatgat caaagccgac ctggcagtgc gtcaggctga
     9061 agccgccaaa tgggcggcta tagccaacta cctgccccag cttaatgtaa acattaatta
     9121 caataactac ctgggcgctt cgatcgaaat tttcggaatg gacattccca tggacccctc
     9181 gagcaggctg acgctgcagg cttcgcaatt gatttttaac gctaatgcca tcctgggcat
     9241 gcagttgagc gagctaagcc gccggatgtc ggaactggga agcaatcaat cggaactggc
     9301 agttaaacaa aacgtgaggt ccacctacta ctccattttg atgatggaaa acaaccggct
     9361 tttgctcaaa aaaaatgtgg aaaatatgcg caatctagct aaggctacca gggctaaagt
     9421 ggaggtgggc ataggcgaac agaccgaagc cgacgaaatt gaaatagtgc tggccaatct
     9481 ggagaacacc ctgaaacaaa ccgaaagaac catagaattg acctataatt ccatgcggct
     9541 actcttgggg ctgaacgccg aggatgggat agaactgagc gacagactgc aggatgttac
     9601 cgacaaacaa gctatgtttg ccttgctggc agccggtttt gatccggccg aaaacgtcga
     9661 tatgaaggca ggacagctca atctggaaat caacaaaaaa caagtaaagt cggccatcgc
     9721 cggttattta ccgactattt cggccgtata tcagcgcaac gagattttgc tgaaaacggg
     9781 ttttgatccg accaccaaaa acacgctggt tctcacggcc agcatgcctc tgttctgcag
     9841 cggcaaaacc accgccgaac tgaaaaaagc ccaactagcc tacgaatcgg cgcaggaaga
     9901 tctggaactt atgcaaaaac agttgttgat tcaggacaaa cagttgcgtt ttaacttgaa
     9961 atctgctctg gaatcttacg aaatacaaga acgcaacata gaggtttcga agcgcatgtt
    10021 cgacaacgtg accaagaaat tcgaacaggg cttatcgtcc agcctcgaag tgaccaacgc
    10081 cagcaacaac ctgcttaccg cacaaagcaa ttacatccag tcggccatga gcttactcaa
    10141 cgcaagcgac gctttgaaaa aattgctggg aacattataa acggttcaat catcattatc
    10201 atcagtagca gcaacaacaa cagcaacaac aacagcaaca acaacagcaa caacagcagc
    10261 aacaacaaca acaaaaacat catctgcaac aacaacagga aataaaaaca ggaaataaaa
    10321 caataaaaaa catctgcaat gaaaaatccg ctttctgcac ttccggccct gctaattatc
    10381 gcaacggggc tgtatgcctg ctccggtgct tccaaatcgc gggaagcaca attagaaaca
    10441 actcaggaaa agatcgaaaa tgtcaaagta acaaccctga gttcgcgtat ggtgggcaat
    10501 accctggaat tcccggctaa taccagggct tacgaacggg tcaatctggg gccggtatcg
    10561 ccgggccgta ttgaaaaaat ttacgttgag atgggcgacc gtgtcaaaaa aggacaattg
    10621 cttattcaaa tggatcagac cgcgttgctt caggcacgca ttcagatcgc caattttgag
    10681 gaagaccaca ggcgctatga agcgctcaaa gagagcggag ctatttcgca acaggtttac
    10741 gaccagtcgt tggccaacct gaacatgcaa aaaaccaacc tggactacct ggaaaaaaac
    10801 acccggatta aagcgccctt cgacgggatc atcgccctgc gtaatttcga aaacggcgaa
    10861 ctttacccgg gcggttcggc catactgacg ctgatggaaa tcgataaact caaagcagac
    10921 atcaacgtac ccgaaagcta ttatccactg atcaaaaaag ggatgaaggc cgaagtattt
    10981 tgcgatacct accccggtcg ccaattcgaa gccgaagtca atttgattgc acctacggtc
    11041 aatccggcca gccgttcttt tgccgttgaa ataaaaattc ccaacaccaa agaatggctc
    11101 aagccaggta tgtttgttcg catccatata gatctgggag tgagcgaagc catcttggtg
    11161 ccctcacagg ccgtacttaa actgcaaggc tccaacgaac gctttgtatt cctgcaccgc
    11221 aacggcctgg ctcaaagagt gcccgtacag attggccggc gcttcgacga cgaggtggaa
    11281 ctgatctccg accgcattgt agcaggcgac caattggtgg tgttgggaca ggccaggctc
    11341 agagatggtg ttaaattgaa tgtgatcgaa taattcaagc gagatgagta tctataaaag
    11401 taccgtaaat aaaccggtta ccacactgat ggtctttgcg gccgtcatgg tggcaggctt
    11461 gtacgccctg taccatttgc ctattgacca gtttcccgaa atagaaccgc cctttgtgac
    11521 ggttatgacc acctactcag gggccaatgc ctccgagata gaaaccaata taaccgaact
    11581 gctcgaagac gctttcaaca gcgtgca
//
LOCUS       DAOSTD010000057        11567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_5529_length_11567_cov_14.913308, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000057 DAOSTD010000000
VERSION     DAOSTD010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11567
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5529_length_11567_cov_14.913308"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..189)
                     /locus_tag="PLI37_05100"
     tRNA            complement(119..189)
                     /locus_tag="PLI37_05100"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(155..157),aa:Cys,seq:gca)
     gene            complement(429..626)
                     /locus_tag="PLI37_05105"
     CDS             complement(429..626)
                     /locus_tag="PLI37_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOQ58421.1"
                     /translation="MKEKIGNNAGSVWTVLSAAGGRKSLKEVKKLTKLTDKDLYAALG
                     WLARENKIVFAEEDEIFVALS"
     gene            complement(632..1084)
                     /locus_tag="PLI37_05110"
     CDS             complement(632..1084)
                     /locus_tag="PLI37_05110"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOQ58422.1"
                     /translation="MKAIKKSLGLLFVVVLVFSSCKSNKEAYSATYERAKAKQEVQAQ
                     INEEQNERVEVIEKTGVKEAAEKIEVIGPDSEKGPYGVVVGSFINRTNAENLRKKMVD
                     QGYNAVLGQNDKLMFRVIVAFYEVKADAQAKVRELSKDFPDAWILIKE"
     gene            1211..2515
                     /locus_tag="PLI37_05115"
     CDS             1211..2515
                     /locus_tag="PLI37_05115"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOQ58423.1"
                     /translation="MNYSQALDYLLSQLPFFQAEGKKAYKPGLDNIRQLDAWAGHPHR
                     RFPCIHVGGTNGKGSVCHMLASVLQEAGYRTGLYTSPHLKDFRERIRINGRCISETYV
                     SEFTTAYVDAKKAGLILEADFKPSFFELTTTMAFSYFADNNVDVAVIEVGLGGRLDST
                     NIIDPVLALITSISLDHMNLLGNNVLSIAGEKAGIIKSGRPAVVGINPSEVYRVLRKR
                     ALQEQAPFYLAEDLLQLQGETVNKGTKLKVKSGVWQELNTFTCGLAGQYIPENATLVL
                     AALSLLKQDFIQIDAPALLRGFEQVVPNTGIRGRWEILQERPLCICDTGHNEAGIRVV
                     TKQLAATPHKQLHIVLGVVNDKDIGAMLELLPQKALYYFTKAAVPRALPETELAALAA
                     ARNLKGQSYPTVTEAKAAALKAAAADDLVFIGGSNFIVAEAL"
     gene            complement(2512..3246)
                     /locus_tag="PLI37_05120"
     CDS             complement(2512..3246)
                     /locus_tag="PLI37_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ58424.1"
                     /translation="MGRAFEYRKARKMKRWGNMARTFTKIGKEISSCVKESGPDPDSN
                     PRLRVLIQNAKAANMPKENVERAIKKAVSKDYVDFKEMVYEGYGPFGIAILVETQTDN
                     TTRTVASVRSAFNRYGGSLGTTGSLSFMFERKAVFKIKPKEDLDPDELELELIDYGVD
                     ELETEDEDIVIYGEPESFARIQKYLEENEFEIVSAEFERFPNETKELTPEQEATINKL
                     IERLEEDEDVSNVYHNMKESLSEEDA"
     gene            complement(3259..5721)
                     /gene="pheT"
                     /locus_tag="PLI37_05125"
     CDS             complement(3259..5721)
                     /gene="pheT"
                     /locus_tag="PLI37_05125"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOQ58425.1"
                     /translation="MLISFDWLKEFVDIDISAAECAKVLTSIGLENGSLEMVESIRGG
                     LEGLVTGEILTCVPHPNSDHLHLTTVNIGGPEPLPIVCGAPNVAAGQKVIVATVGTKL
                     YMGDREMTIKRSKIRGEESFGMICAEDEIGIGDSHEGIIVLPADTPVGLPASKYYGLE
                     SKAVLEVDVTPNRSDALSHYGVARDLAAWFKTQGKNVELRRPSVEAFRVDNRDLNIGV
                     EILNPEACPRYCGLTMTGVRVKESPKWLQDRLRLIGQHPINNVVDITNYLLHETGQPL
                     HAFDAAKIKGNKVVVRTLPEGTPFVTLDGVERKLSAQDLMICNAEEGMCVAGVFGGLD
                     SGVTEQTTEVFLESAYFNPVWVRKTARRMGLNTDASYRFERGVDPDNTLYVLKRAALM
                     IKELTGAQIAGEIVDCYPTKIEDFVLDFSYEKCHKLIGEVIPPAKIKEILAALEIKIL
                     EEHTDRLKLQVPTYRVDVQRDVDVIEDILRIYGYNHIQTGETLRTSLSYTRHPDSNHL
                     QQIVSEQLTASGFNEVINNSLVSSAHYEGLSTFPPKACVRLLNPLSKDLNVMRQSLLF
                     GGLQNLSYNINRKNPDLRLYEFGNCYQYLDEKKAAGNNLAAYDESMHLGLWLCGNRNS
                     IHWMQPASALSFYDLKAYVHHILLRLGLEPEKLEISEISPEDIFTQALCYSSKAGKTL
                     LTMGIVNAAILQAFDLDTEVYYADIHWDNLLKVLKTNEISFKELPKFPEVRRDLALLL
                     DHSVSFSMIENIARASEKQLLKSLYLFDVYEGKNLPAGKKSYAVTFVLRDENQTLTDE
                     RIDACMQKIIRNLQSQLGASLR"
     gene            complement(5725..6627)
                     /gene="dapA"
                     /locus_tag="PLI37_05130"
     CDS             complement(5725..6627)
                     /gene="dapA"
                     /locus_tag="PLI37_05130"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOQ58426.1"
                     /translation="MKISINNKLRGMGVALITPFNEDDSIDFDALGRLVDYQLHNKTD
                     YLVVLGTTAETPTLSDEEKIAVKDFVIQRVNGRLPIVLGVGGNCTRSLVHQLETNSYD
                     GISALLSVTPYYNKPSQEGLYQHYKALSAATDKPILLYNVPGRTGVNMTAETTLRLAG
                     ECANIVAVKEASGNIVQVNEIIKKKPSDFMVISGDDAVTFPYITLGASGVISVIGNAF
                     PKEFSRMVRLALNGDYGNALEIHQRFTELFDLLFVDGNPAGVKCMLHAMGYIKNKLRL
                     PLVPTRITTYEKIREVLVQLSIKC"
     gene            complement(6672..7190)
                     /locus_tag="PLI37_05135"
     CDS             complement(6672..7190)
                     /locus_tag="PLI37_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58427.1"
                     /translation="MVDFFLKRKIKQLRRQHSRNKRFMNYADLRRICILFDVADYEHI
                     VPLVDELLADGKNVIAWTYAARGVIAKDYPAYYNVISDRDLTFCYFPKKYLMNRYLGY
                     PADLLIDLCYTPHILNEYLVAVSPALYRLGLKTADPLWYDLIVDISGQEKEEMEKNAA
                     SVLFYLKSLRVK"
     gene            complement(7502..9502)
                     /gene="ligA"
                     /locus_tag="PLI37_05140"
                     /pseudo
     CDS             complement(7502..9502)
                     /gene="ligA"
                     /locus_tag="PLI37_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267567.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
     gene            complement(9506..11452)
                     /locus_tag="PLI37_05145"
     CDS             complement(9506..11452)
                     /locus_tag="PLI37_05145"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOQ58428.1"
                     /translation="MSCFNVVPVFAQGRPGGSSEKKETVALRIRTSSIDETLGEKKAA
                     SLDDTLSLNFQNMAFPEKKNTVAASHLGNIGSPFQSKIYTDRKAKHRFLFLQPYSQWK
                     TSMDEWLFFNTTRPYTNAHYITTVGNDYSQEEDFRFFFTVNADKYLNFGAEYRSTSAR
                     GYYSNTATRSKIARLFGNYQSPRYEAFMRATYNRFENYENGGITDDRYITHPLEMSGG
                     YREYESLNIPVHLSNTFNLATCRDVFYNHKYNIGFKRQEVTEDDTVEVFVPVTAFIHT
                     FHLDNGWKKYDSRTANPAFYDTAYIDNTHTADTAALTTVRNTFGLSLREGFHSWVKFG
                     LTAYAEHEYRQYARISSAAIPDSSESDPFKQMVYHRENLVWLGGRLASTGDSALRFNV
                     DAKICMLGSDYAGNFEIKGDVSSRFRLWGRPVYLAADGFVVSRMPDYFLRRYYSNHFD
                     WNNAFRNEYKTRISGSLSIPALGFDLSAGVENISNYVYFDRKALPAQYDGQIQVLFAA
                     WKQHLGYGIINLDNEAVFQLSGKEEILPLPRLSLYTNLYAKTKLFDVLTLQAGVDCRY
                     HTAYYVPKYMPATGQFYLQDELKIGNYPFMNAYANFHLKRARFFVMYTHGSRWFADPN
                     YFSLPHYPLNPAGIKAGLSWNFYD"
BASE COUNT         2852 a         2886 c         2787 g         3042 t
ORIGIN      
        1 ttcttgttta aaatatggtt ttacaacggc agacagccta taaacggaag ctggtgccgc
       61 aaaagtcaaa aaaacagtct ccttactcaa gagactgtcg tttactaaat tacctaagag
      121 gttcctggcg gattcgaacc gccgtaagcg gttttgcaga ccgttaccta gccactcggt
      181 caaggaaccg gcttccggca taagccggat tttcagaacg caaaggtatg ctatagtttg
      241 gttttgcaca aggactccgt gcatttttta tccttcagcg gacaagaagc acaggccgtg
      301 ttgcagcttt gtcgttgaga agcagtccta aaaaaaagga cgagcctttg tgccagccag
      361 acaaaactaa gcccgacaag cagacaaacc gctatttcct gaaaaagaag catacctaaa
      421 actcaagatt agctcaaagc tacaaaaatt tcgtcttctt cggcgaatac tattttgttc
      481 tcacgagcca gccaacccaa agctgcataa agatctttgt cggtcagctt ggtcaatttc
      541 ttaacttctt ttaggctttt acgcccgccg gcagcgctca acactgtcca aacggagccg
      601 gcattgtttc caattttttc tttcataata cctattcttt aattaaaatc catgcatcgg
      661 gaaaatcttt gctgagttca cgaaccttgg cctgagcatc ggccttgact tcgtaaaatg
      721 caactattac cctgaacatc aatttgtcgt tttgtcctaa aacagcgttg taaccctggt
      781 caaccatctt tttacgtaaa ttctctgcgt tggtgcgatt gataaaactg cccacaacca
      841 ccccataagg gcctttttca ctgtccggtc cgatgacttc tatcttttcg gcggcctctt
      901 tgacgccggt tttttcgatc acttccacgc gttcattctg ctcttcgttt atctgagcct
      961 gaacttcctg tttggctttg gcacgttcgt aagtggcact gtatgcttct ttgttcgatt
     1021 tgcaggaact aaacaccagc acgaccacaa acaacaagcc aaggcttttc ttaatggctt
     1081 tcataaccgg aaatgtttaa aattcgggac aaaagtacgc atttttattt taaattaaaa
     1141 agccgtttta cattaaatgg aaatccgttc aaaaagctgt attttcgcaa acccaactca
     1201 tttgcccttt atgaactact cgcaagcatt ggattacctc ctgagtcaac tgcccttttt
     1261 tcaggccgaa gggaaaaaag cctacaagcc ggggctggat aatatccggc aactggatgc
     1321 ctgggccggt catccccacc ggcgttttcc ctgcatccac gtaggcggca ccaacggcaa
     1381 aggcagcgtc tgtcatatgc tggcctcggt cttgcaagaa gccggctatc gcacaggctt
     1441 gtacacctcg ccccatttaa aagacttcag ggagcgtatc cgcatcaacg ggcgatgtat
     1501 ttcggaaact tatgtcagcg aatttaccac agcctacgta gatgccaaaa aggccgggtt
     1561 aatcttagag gcagatttca agccttcgtt ttttgaactt accaccacca tggccttcag
     1621 ttattttgcc gataataacg tagatgtggc cgtcattgag gtcggcctgg gcggacgcct
     1681 cgacagtacc aatattattg atcccgttct ggcgcttatc accagtatca gcctcgatca
     1741 catgaacctg ctgggcaaca atgtccttag catcgcaggc gaaaaagcgg gcatcattaa
     1801 atcgggcagg ccggcagtgg tgggaataaa tccctccgaa gtgtaccggg tgttacgcaa
     1861 gcgcgccctg caagagcaag ctccctttta cctggccgaa gatctgctgc aattgcaggg
     1921 agagacggtc aataagggaa caaagcttaa agtaaaatcc ggagtctggc aggaattgaa
     1981 cacttttacc tgcggactgg ccggccaata cattcccgaa aacgccactt tggtactggc
     2041 cgcccttagt ctgttgaagc aagactttat tcaaatcgac gcccctgccc tgctcagagg
     2101 attcgaacag gtggtaccca acacaggtat cagaggccgt tgggaaatcc ttcaggaaag
     2161 gcctttatgc atttgcgata caggacacaa tgaagccgga atacgggtgg taaccaagca
     2221 attggccgcc acaccccaca aacaactgca tatagtattg ggcgtggtca acgacaaaga
     2281 catcggagcc atgctggaac ttctgcccca aaaggctctt tattatttta ccaaagcagc
     2341 cgtacccaga gccttgcccg aaacggaact ggctgcgttg gcggcagcgc gaaacttaaa
     2401 aggacagagc tatcccacgg tgacagaagc aaaagcggcc gctttaaaag cagccgctgc
     2461 agacgatttg gtttttatag gaggcagcaa cttcatagtt gccgaagctc tttaagcgtc
     2521 ttcttccgaa aggctttctt tcatgttgtg ataaacgttg gagacgtctt cgtcttcttc
     2581 gagcctctcg atcaatttat tgatggtggc ctcctgttcg ggtgtcaatt ctttggtctc
     2641 gttgggaaaa cgttcaaatt cggccgacac aatttcaaat tcattttctt cgaggtactt
     2701 ttgaatgcgg gcaaaggatt caggttcgcc gtagataacg atgtcttcgt cttccgtttc
     2761 aagttcatcc acaccgtagt cgattaattc cagttccaat tcgtccgggt cgaggtcttc
     2821 tttgggtttt attttgaaga ccgccttgcg ctcgaacata aaggaaaggc tgcccgttgt
     2881 gcccagagag cctccgtaac gattaaaggc actgcgaacc gaagctaccg tacgggtggt
     2941 gttgtcggtc tgagtttcga ccaaaatggc gataccaaag gggccatagc cttcgtaaac
     3001 catttccttg aaatcgacat agtctttgga aacagctttc ttgatagctc tttccacgtt
     3061 ttctttgggc atattggccg ctttggcgtt ttggatcaaa accctcagac gcgggttgga
     3121 gtcgggatcg gggccggatt ctttgacgca actcgaaatt tcttttccga ttttggtaaa
     3181 agtacgggcc atattgcccc aacgtttcat ttttctggct tttctgtatt caaaggctct
     3241 tcccataagg caggtatttt atctcaggct cgcgcccaat tgcgattgta gattccggat
     3301 gattttttgc atgcaggcat cgatgcgttc atcggtgagt gtttggtttt cgtcccttaa
     3361 aacaaaagtc acggcgtagg attttttgcc cgcaggcagg ttctttcctt cgtacacatc
     3421 aaacagataa agcgatttca gtaattgctt ttcggaagct ctagcgatgt tttctatcat
     3481 cgaaaaagac accgaatggt cgagcaacaa agccaaatcc cggcggactt cggggaattt
     3541 aggcaattcc ttgaacgaaa tctcattcgt tttcaggacc ttgagcagat tgtcccaatg
     3601 tatatcggcg taatacacct ccgtatccag gtcgaaggct tgcagtatcg cagcatttac
     3661 tatacccatc gtaagcaggg tcttcccggc tttgctgctg tagcaaaggg cttgtgtaaa
     3721 gatatcctcc gggctgattt cgcttatttc cagtttttcg ggttccaggc ccaggcgtaa
     3781 caaaatatgg tgcacataag ctttgaggtc gtagaaactc agagctgagg caggctgcat
     3841 ccaatgaatg gaattgcggt taccgcagag ccacagcccc aggtgcatgc tttcgtcgta
     3901 tgcggccagg ttgttgccgg ctgctttctt ttcgtcgagg tattgataac aattgccgaa
     3961 ttcatacaag cgcaaatccg gatttttacg gttaatgttg tatgagaggt tttgcaaacc
     4021 gccaaacaag aggctttgtc tcattacgtt caagtcttta ctcagaggat tcagcaggcg
     4081 cacacaggct ttcgggggga aagtgctcaa tccttcgtaa tgagccgacg acaccaacga
     4141 attgttgatc acttcgttga agcccgatgc ggtcagttgc tccgaaacaa tctgctgcaa
     4201 gtggttactg tcggggtgcc gggtgtaaga gagcgaggtt ctcagagttt cgccggtctg
     4261 aatatggttg taaccgtaaa tacgcaaaat atcttcgatc acgtccacat cacgctgcac
     4321 atccaccctg taggtaggca cctgcagttt tagccggtca gtatgttctt caagaatctt
     4381 aatctccaaa gcagcgagga tttctttgat cttggccgga ggtataactt ctccgataag
     4441 cttatggcat ttttcgtaac tgaaatccaa tacaaagtct tctattttgg taggatagca
     4501 atccactatt tcaccggcta tctgagctcc ggtcagctct ttgatcatca gggcggcccg
     4561 cttgagcaca tagagcgtat tgtccggatc cacgcctctt tcgaaacggt aagaagcatc
     4621 ggtgttcaga cccatcctgc gggcggtttt tctcacccag acagggttga aataagcact
     4681 ttccaaaaac acttccgtag tctgttccgt tacgcccgaa tccaggccgc cgaaaacacc
     4741 ggccacgcac atgccttctt cggcgttgca gatcatcagg tcctgagcgc tcaacttacg
     4801 ttccacaccg tcgagggtta caaaaggagt gccctcgggc aaggtacgaa ccaccacttt
     4861 gttccccttt attttggctg catcgaaggc gtgcaaaggc tgaccggttt cgtgtaacag
     4921 ataattggtg atgtccacca cattgttgat gggatgctgt cctatcaggc gcaggcgatc
     4981 ctgcagccat ttgggcgatt cctttacccg gactccggtc atggttaatc cgcaataacg
     5041 gggacaggct tcggggttga gtatttctac cccaatgttc aagtctctgt tgtctacccg
     5101 gaaagcctcg accgatggcc tgcggagctc aacgttttta ccctgggttt tgaaccaggc
     5161 cgccaaatcg cgggccacac cgtaatgcga caaagcgtcc gaacggttgg gagttacgtc
     5221 aacttcgagc acggctttgc tctccagtcc gtagtattta cttgcgggca ggcccacggg
     5281 agtgtctgca ggcaaaacga tgatgccttc gtggctgtcg ccaataccga tttcgtcttc
     5341 ggcacagatc attccgaagg actcttcgcc cctgatttta gaacgtttta tggtcatttc
     5401 ccggtcgccc atatagagct tggtgcccac agtggccaca atcacttttt ggcctgcagc
     5461 cacgttggga gctccgcaca caatggggag cggttcggga ccaccgatgt ttaccgtggt
     5521 caagtgtaaa tggtcggaat tggggtgagg cacacaagtc aatatttctc ctgtcaccag
     5581 gccttcgagg ccgcccctga tactttccac catctccaat gagccgtttt caagcccaat
     5641 ggaagtcagt actttagcac attcagcggc tgagatgtct atatcgacaa attcttttag
     5701 ccaatcaaac gaaataagca tacatcaaca ttttatggat aattgcacca gcacttccct
     5761 tattttctca taggtagtga ttctggtcgg caccagcggc agcctcagtt tgttcttgat
     5821 atagcccatg gcgtgcagca tgcatttaac accggccgga ttgccgtcaa caaacaagag
     5881 gtcgaacaat tccgtaaatc gctggtgtat ttccagtgca ttgccgtaat cgccgttgag
     5941 ggccagacgt accatgcggc tgaattcctt ggggaaagca ttgccgataa ccgaaatcac
     6001 gcccgaggcc cctaaagtaa tgtaggggaa ggtcacggcg tcgtcgcccg aaataaccat
     6061 aaaatcggaa ggcttctttt tgatgatttc gttcacctga acaatattac ccgaagcctc
     6121 tttgacggca acgatgttgg cacattcccc ggctaaacgc aaggtggttt cggcggtcat
     6181 gtttaccccg gtacgaccgg gaacattgta caataagatg ggtttatccg tagcggcgga
     6241 taaagcttta taatgctgat acaatccttc ctgagagggt ttgttgtaat agggcgtcac
     6301 cgaaagcagg gcggaaatgc cgtcgtaact attggtctcc aattgatgaa ccaggctgcg
     6361 cgtacaattg cccccaactc ccaacactat gggaagccgg ccgttaaccc gctgtatcac
     6421 aaagtctttg acagcgatct tttcttcgtc gctgagggtg ggtgtctcgg ccgtagtgcc
     6481 gagaaccacc aggtaatcgg ttttgttgtg caactgataa tccaccaggc ggcccagagc
     6541 gtcaaaatcg atactgtcgt cttcgttgaa aggtgttatc agggcaacac ccatccccct
     6601 caatttattg tttatggaaa ttttcataca ctctgggttg cagcaatccg aggcgcaaag
     6661 gtataaaaaa cctacttaac tcgtaaactc ttaagataaa aaagcacgct ggcggcgttt
     6721 ttttccatct cttctttttc ctgcccgctg atgtccacta tcagatcgta ccagagggga
     6781 tcggcggttt ttaatcccag gcggtacaaa gccggcgata cggccaccaa atactcgtta
     6841 aggatgtggg gagtatagca gagatcgatc aggagatcgg ccggataacc caggtaacga
     6901 ttcatcaggt attttttggg gaaatagcaa aaagtcaggt ccctgtcgct gatcacgttg
     6961 taataggcgg gataatcctt agcaatcacc cctctcgcag cataggtcca ggctattaca
     7021 tttttgccgt cggccagcag ttcatcaacc agaggaacga tatgttcgta atcggccaca
     7081 tcgaacaaaa tacagattct ccgcaagtcg gcataattca taaaacgctt gttacggctg
     7141 tgttggcgtc ttaattgttt gatctttcgt tttaaaaaga aatctaccat agtctcctat
     7201 tctgtgttta gtattagagt tcgtcaaaaa gaccggggct cgagatggag ccggtgccgg
     7261 attttttgct ctgcttgttc cggtttgaca ggggagggct ctcggcaggg gcagtagttg
     7321 gaacagactc agcaggggtt gcagggccgg aaaccacgtc ccggcccgga tcggcgactg
     7381 agccggaacc ggtgccgctg cctgaaacag cgcctgagcc gggaaccgca tccgacccga
     7441 cctcggtgtc tgagccggag ccggagccgg tgtctgagcc ggtgcaggtg tccgagccgg
     7501 cggccagtaa ggccagaaat tccttttcgg acatcagttt cagacccagc gatttggcct
     7561 tttcgagttt ggaaggcccc atgttttcgc cggccaaaat aaagtctgtt ttggcgctga
     7621 ccgaggaagc gattcttcct ccctcctgct ctatcagctc tttgtattcg tctcggctgt
     7681 gtttttcgaa agttccgctg atcactacgg tctgattttt caacaaacct tcggttttct
     7741 gtgcggtctt gtccgattca aactgcaaac ccagttgccg gaactcttcc aacatggccc
     7801 gattggaagc atcggcaaaa aaggccaata cactttcggc tattttttcg cccacctcct
     7861 ccacctgcag gagttcttcg taagtggccc gacgtagcgc atccattgag cccaaagtac
     7921 gcgccagttt tttagcgacg gtttccccca caaaacgtat acccagagca aacaagagtc
     7981 tttcgaaggg caccgaaacc gaggcggtta tgctatttat caaattatcg gccgacttct
     8041 cgcccatcct ttcgagcccc gtcagttgat cctttcgcag acgatataaa tcggaagctt
     8101 tgttgaccag acctttctga tacaataaat ccactgtttc ttcgcccaaa ccttcgatat
     8161 tcatcgcctt acggtgaaca aaatgcaaaa tcctgccttt gatttggggc ggacaacccg
     8221 tagtgttggg gcaataatga gcggcttcgc cttcgatacg ctgcaaaggg ctgccgcatt
     8281 ccggacaacg ctcgataaat tgcaccggct ccgatgctcc gacttttctg gagtccttat
     8341 ctactcctac aatcttggga atgatctctc cgcctttttc caccaaaacc cggtcgccca
     8401 aatgcaaatc cagttccctg ataaaatcgg cgttgtgcag cgaagctctt tttaccaccg
     8461 tgcccgataa aagcacgggc tccaggttag ccaccggtgt aacggctcct gttctaccca
     8521 cctgaaaact caatgatttc aattgggtca gtgcttgttc ggcctgaaat ttataagcga
     8581 tggcccaacg gggcgatttg gcggtagaac ccaattgctc ttgctgacgt aaggaattga
     8641 ccttgataac aatgccatcg gtagccaccg gcagtttttt gcgttctaca tcccaataac
     8701 gtataaagtc gaacacctcg tcgaggctac tgcatttaca tgtatgttcc gaaacacgca
     8761 acccccagct tcgggctttt tgcaggtttt cgtaatgtcc ctcaaagggt aggtgttcgc
     8821 ccatcacggc gtagaacaag gcttcgagct tacgcgaagc caccgttgct gaattttgca
     8881 atttcaatgt gcccgaagcg gcgtttctgg gattggcaaa aaggttttct tcctgctttt
     8941 ctcgttcttc gttcagcgct tcgaacactt cccagggcat cagcacttcg cccctgatct
     9001 cgaccaattg cggccagtct tctcctctga gtttgagggg aatgctgcgg atggtacgaa
     9061 tgttttgggt cacgttgtcg cctttttggc cgtcacccct ggtcagggct ttgatcaaaa
     9121 taccctcttc gtaggtcagc gatatggaag tgccgtcaaa tttcagttca cagaccaatt
     9181 cgggctcttc gtcgaggctt ttgagggttc tgtcgtaaaa atcacggact tcttcttcgg
     9241 aataggtatt gctcaacgag agcatgggat aacgatgctc ctcttgtgta aaagcccggt
     9301 tcaaatcact gcccacgcgc tgagtgggcg aatcgggggt gatcagctcc ggatgagctt
     9361 gttcgagctc ttgcaaacgg cgcattttga cgtcgtaatc aaaatccttc atgctgggag
     9421 ccgacagttg ataatattcg taatcggcct gatgaagctc ccggcgcaat tgctgtattt
     9481 cttgttctat aagagtattc atgggctaat cgtaaaaatt ccaggacaag ccggccttga
     9541 taccggcggg attgagcgga taatgcggca gggaaaaata attcggatcg gcaaaccagc
     9601 gactgccatg agtatacatc acaaaaaagc gtgcccgttt caggtgaaaa ttggcgtagg
     9661 cattcataaa aggataattc cctattttca actcgtcctg tagataaaac tgtccggtgg
     9721 caggcatgta cttgggaacg taataagctg tgtgatagcg gcaatcgaca ccggcctgca
     9781 aagtcaggac atcaaacaac ttggttttag cgtaaagatt ggtgtacaaa gacaggcggg
     9841 gcaaaggcag aatctcttct ttaccggaga gttgaaaaac ggcttcgttg tcgagattga
     9901 tgatgccgta ccccagatgt tgtttccagg ccgcaaacaa cacctgtatc tgaccgtcgt
     9961 attgagccgg caaagccttt ctgtcgaagt agacgtaatt gctgatgttt tccacgccgg
    10021 ccgacagatc aaaacccaaa gcaggtatgg acaggctgcc cgaaatccgg gttttgtatt
    10081 cgttgcgaaa agcattgttc caatcgaaat ggttggaata ataccggcgc aaaaaataat
    10141 cgggcatacg gctcacaaca aatccgtcgg cagcaagata aaccggcctg ccccagagtc
    10201 tgaaacggct ggagacatcg cctttgattt caaaattgcc ggcgtagtcg ctccccaaca
    10261 tgcagatttt ggcatccaca ttaaaacgta aggccgaatc accggtcgat gcgaggcggc
    10321 cgcccagcca aaccaggttt tccctgtgat agaccatttg tttgaaagga tccgattcgc
    10381 tgctgtcggg tatggcggcc gacgaaatac gggcgtattg acggtattcg tgttcggcat
    10441 aggcggtgag tccgaatttt acccaggaat gaaagccttc gcgcagagac agcccaaagg
    10501 tattgcgtac ggtggtcagc gcagccgtgt cggccgtatg ggtattgtct atgtaggccg
    10561 tgtcgtaaaa agcaggattg gccgtacggg agtcgtattt tttccagccg ttgtccaaat
    10621 ggaaagtgtg aataaaggcc gttaccggca cgaacacctc caccgtatcg tcctctgtca
    10681 cttcctgccg tttaaagcca atgttgtact tgtgattata aaaaacatcg cggcaagtgg
    10741 ccaggttgaa tgtattcgac aaatgaaccg gaatattaag gctttcgtat tcgcggtatc
    10801 cgccgctcat ttcgagggga tgggtaatat agcggtcatc ggtaatacct ccgttttcgt
    10861 aattttcaaa gcgattataa gtagcccgca taaaagcctc gtagcggggc gactgataat
    10921 taccgaacaa gcgggcaatt ttactccggg tggccgtatt ggaataatag cccctggcgc
    10981 tggtggaacg gtattcggct ccgaaattaa ggtatttgtc ggcattgacg gtaaagaaaa
    11041 agcggaaatc ctcttcctgg gaataatcgt tacctaccgt tgtaatatag tgagcgttgg
    11101 tataaggacg ggtggtgtta aagaaaagcc attcgtccat ggaagttttc cactgactgt
    11161 aaggttgtaa aaacaaaaaa cgatgcttgg ccttacgatc ggtgtaaatc tttgattgaa
    11221 aaggcgagcc tatattgccc aaatgagaag cggccaccgt gtttttcttt tcgggaaatg
    11281 ccatgttctg aaaattcaga ctcagagtgt cgtccaggga agcagctttt ttttcaccca
    11341 gggtttcgtc tatgcttgag gtgcgaattc tcaaggctac cgtctctttt ttttcgctgc
    11401 tgccgccggg cctgccctgg gcaaaaaccg gaacaacgtt gaaacacgac accagcatta
    11461 agattgaaaa ccttaagaaa actctatgca taaccgggat atacaattgc tgcaaaaata
    11521 gaatgaatat tccataatgg gaaatcccgt tccggcaaat tttaccg
//
LOCUS       DAOSTD010000058        11517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_5555_length_11517_cov_14.876985, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000058 DAOSTD010000000
VERSION     DAOSTD010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11517
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5555_length_11517_cov_14.876985"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLI37_05150"
     CDS             <1..207
                     /locus_tag="PLI37_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58429.1"
                     /translation="ISGTFWMAAAYLAVLPLSLLIFFRSKVLIIKLYNRTRRFWLKVR
                     KNPLLAASEKLRREIVEVMDKLIF"
     gene            311..1576
                     /locus_tag="PLI37_05155"
     CDS             311..1576
                     /locus_tag="PLI37_05155"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOQ58430.1"
                     /translation="MDVLLGLQWGDEGKGKVVDVLTPGYEVVCRFQGGPNAGHTLEFE
                     GHKYVLRSIPSGIFQGDKINVVGNGVVIDPALFMGEIDQLAKSGHKLNDRLMISKKAH
                     LILPTHRLLDAANEAAKGQGKIGTTGKGIGPAYTDKVSRNGLRVGDILHDFKRKYKEA
                     AALHLNRLKQMNFPVDSEELAAAEKEWFEGIDCLKQFKLIDSEHFLNKKIGEGKKILC
                     EGAQGTMLDIDFGSYPFVTSSNTVCSGACTGLGVAPRYIDRVFGIFKAYCTRVGSGPF
                     PTELFDENGKLMRDRGNEYGSVTGRPRRCGWIDLVTLRYAVMINGVTDLIMMKSDVLD
                     SFDTIKACVAYRIGGKETENLPFDIQGKLEPVFTELPGWKTDMTKMRSEDEFPEEFNQ
                     YLSFLEDELGVRIKYVSVGPDRAQTIERY"
     gene            1582..2262
                     /locus_tag="PLI37_05160"
     CDS             1582..2262
                     /locus_tag="PLI37_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOQ58431.1"
                     /translation="MIYVIFIGFALLSWIVQSVLKNKFDKYSKIALPSGLSGKEVAEK
                     MLHEHGIYDVKVSHVQGQLTDHYNPLTKTINLSEAVYNSNSIAAASVAAHECGHAVQH
                     ATAYSFLTMRSKLVPVVNFAAGWMHWVLLAGILLVQSFPYLLLFGIVLFGLTTLFSFI
                     TLPVEINASSRAVTWLNKTGITNVSTHPMAVDALKWAAYTYVIAALSSLATLIYYILI
                     FLGGRNRN"
     gene            2269..3645
                     /gene="hisS"
                     /locus_tag="PLI37_05165"
     CDS             2269..3645
                     /gene="hisS"
                     /locus_tag="PLI37_05165"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOQ58432.1"
                     /translation="MAIQKPSIPKGTRDFTPEETAKRNYIFNTIRSAFESYGFQAIET
                     PALEQLSTLMGKYGDEGDKLLFKILNSGDFAGKLSDEELLSRETGRLTRLISEKGLRY
                     DLTVPFARYVVMHRNEIVFPFKRYQIQPVWRADRPQKGRYREFFQCDADVVGSDSLLN
                     EVELLQLLDTVFNDLGIRVCIKLNNRKILAGLAQILGQEERLTDITVAIDKLDKIGLD
                     NVRAELIEKGISAQTVRELEPVLLLQGSNSEKTTALRRYLAASKQGLQGIEELDYILK
                     QAEKLLKNAKLELDLSLARGLNYYTGCIIEVKALDAEMGSIAGGGRYDNLTGVFGLPG
                     VSGVGVSFGADRIYDVLTQLTLFPATSVLTTQLLITNFGEEETAWCLPLAAELRENGF
                     RVEVYPDADKIKKQLSYADSKGIPFVLMAGADEIKQNKVTLKNMHTGQQNLMSPQDLL
                     RFLKTTTL"
     gene            3642..5162
                     /gene="lysS"
                     /locus_tag="PLI37_05170"
                     /pseudo
     CDS             3642..5162
                     /gene="lysS"
                     /locus_tag="PLI37_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
     gene            5205..6206
                     /locus_tag="PLI37_05175"
     CDS             5205..6206
                     /locus_tag="PLI37_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HOQ58433.1"
                     /translation="MEFPDRMCVMGGGSWATAIAKMLLMNPQARINWYMRRASQIRDF
                     KRFGHNNSYLTTIPFDINRIRFYSDINVAVKNSDTLVLATPSPYLKLHLKKLQQDVSD
                     KFILSGIKGIVPDENMLVSQYFNKVYNVPHENIATIAGPCHAEEVALEHLSYLTIACS
                     DKEKARAIARSFECKFVRTTITDDVFGIEYASTLKNIYAIAAGICHGLKYGDNFQAVL
                     LSNAIQETNRFTQAAFPQNRKICESVYLGDLLVTAYSKFSRNRTFGTMIGKGYSVKTA
                     HMEMEMIAEGYYATKCIKEINEELYHVNMPILDAVYNVLYERISPSIEIKLLSESLR"
     gene            6279..7616
                     /locus_tag="PLI37_05180"
     CDS             6279..7616
                     /locus_tag="PLI37_05180"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOQ58434.1"
                     /translation="MKQLEINLTKVYDLVPQSEITALRPACQKAMDLLNNGKGKGSDF
                     LGWLHLPSLTDEKLLSDIQKTADLLREKCEVVVVAGIGGSYLGAKAVIDALNNSFDWL
                     KTKRENPVVVYAGHNISEDYLFELSRFLEDKRFGIINISKSGTTTETALAFRLLKTKL
                     EKQVGKEAAREHIVAITDASKGALRQLAQQEGYKTFVIPDSVGGRYSVLTPVGLLPIA
                     VAGHNIRELLAGALAMEKRSETAFEQNPAALYAAARQALYNKGKKIEILANYHPKLHF
                     VAEWWKQLYGESEGKEHKGLFPAAVDLTTDLHSMGQWIQEGERTIFETVISIVSPNRE
                     VVIPTDTGNLDGLNYLAGKRVDQVNKMAELGTQIAHVDGGVPNIKIELPELNEYHLGE
                     LLYFFEKACGISGYMMGINPFDQPGVEAYKKNMFALLQKPGYEAESAAIRSRL"
     gene            7613..8383
                     /locus_tag="PLI37_05185"
     CDS             7613..8383
                     /locus_tag="PLI37_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOQ58435.1"
                     /translation="MTNTTIPKAVENFLAKQGLTALDLKAVLIDMDGVLFDSMPIHAY
                     TWNKTIAEEGLHMSEEEAYLHEGRTGRSTIEIVFRKHLHRDPTEEEVKRIYGKKAGLF
                     DSMPEAPVMEGAPALLDTIASQGLIRVLVTGSGQKLLLNRVNHHFPGHFSSDLMVTAF
                     DVTRGKPHPEPFLKGMQKAGAQAHQALVIENAPLGVEAAKAAGLFTIAVNTGPLNDTH
                     LNKADLIFHNLNELILAWPELMRSCREISLPGHNCRKP"
     gene            8409..9653
                     /locus_tag="PLI37_05190"
     CDS             8409..9653
                     /locus_tag="PLI37_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOQ58436.1"
                     /translation="MNTKQKIFLCLWLFLPFLLGCCRQPAPTKETHRTVLVYMVADNN
                     LNVYAVQNINSMKQNLNADAMETGRLLIFYVPPFSDTQQLLEIKINTLTGQGDTTVLH
                     EYHFDNTLEKNAMQQVVRDAKKLAPAKAYGLVLWSHAMGWHPSTSALDPGSIDPPTLR
                     SAAENIKDEELIGWFGQDGYYHMEIPVLASALEDQGLEFILFDACFTASVEMLYDLRH
                     CADYLIGSPAEVMGAGYPYKDFVKLVFREDLSTEALCRQLCQAYMTSYRANATHPSAS
                     TVLVKLSEMDSLAVCTRAIFEADPIPVSSIDLEGIQHYELMNPHLFYDLDGYLATVSR
                     YPLFYNAFKNQLNRTVLYKDCTDQIYSIYSSSRWFDVGSYSGLSAYIPRYDLPYSQKI
                     TNLNQAYFQTAWAQATGQTAPR"
     gene            9740..11314
                     /locus_tag="PLI37_05195"
     CDS             9740..11314
                     /locus_tag="PLI37_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOQ58437.1"
                     /translation="MNLKFVFGLALCGLVLSCTRTVSIPTKDQVGAAVLPGEIKPVKA
                     PFEMPVFKKPVFPDVEINIADRGAKAGEKITGIVQSAIDELSAKGGGKVVVPGGTWQS
                     GRLILKSNVNLHFAEGAEVYFSGEVEDYQPAVFTRNEGVEMMSLGALIYAYKQDNIAV
                     TGKGKLIGPPMDCAIREQVLKSGVIENVISADTPVEERIYTGLGGSPVLLPMFISPTS
                     CTNVYIEGVTLERTAFWNVVPVYCDGVVIRGITVNSVGIPRGDGIDVESSRNVLIEYS
                     TLNCGDDCFTMKAGRCEDGMRVNKPTENVVVRYCLAKKGHGGITCGSETAGMIRNLYV
                     HDCVFDETGAGIRFKTRRNRGGGGENIVYERIRMNLTGNAFVWDMLGSRTYVGLLADR
                     LPVRELTPLTPSYKNITAKDIIVERSSQFIKITGIPETPLTNLRLENVKAENCERLIQ
                     LRDVDQIHLKNVQVSSDDNNINVLDGRNILFEKVTFSNPEGNIVCLTEGELCRNIRFE
                     ECSPGKPENWEGTVLE"
     gene            complement(11395..11466)
                     /locus_tag="PLI37_05200"
     tRNA            complement(11395..11466)
                     /locus_tag="PLI37_05200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11431..11433),aa:Arg,seq:ccg)
BASE COUNT         3110 a         2880 c         2823 g         2704 t
ORIGIN      
        1 ataagcggca ctttctggat ggcggcggcc tacctggctg tacttccctt gagtctgctc
       61 attttcttcc gatccaaagt gttgattata aaactctaca accgcactcg ccgcttctgg
      121 ctcaaagtaa ggaaaaaccc tctgttagca gcctccgaaa aacttcgtcg tgaaatagtt
      181 gaggttatgg ataaactgat attctaaaat attttttatc tttgtagccg gttttttttg
      241 agagaaaagc acgaacatag ctatgttcgt gcccttttat gtgcacacta ctggtaatca
      301 ttattttatc atggatgtat tattaggcct ccaatgggga gacgagggaa aaggcaaggt
      361 ggtggacgtg ctgacacccg gttatgaagt ggtatgccgt tttcagggcg gccccaatgc
      421 cgggcacaca ctggaattcg aaggacataa atacgtactc cgttccattc cttcgggtat
      481 tttccagggg gacaaaatca atgtggtggg caatggtgtg gtgattgatc ctgctttgtt
      541 catgggcgaa atcgatcaac ttgccaagag tgggcacaag ctgaacgatc gattgatgat
      601 ttccaaaaaa gctcatctga ttttgcctac ccaccgtctg ctggatgcag ctaacgaagc
      661 cgccaaaggt cagggcaaaa tcggcaccac aggcaaaggc attggccctg cctataccga
      721 caaagtgagc cgcaacgggc tcagggtggg cgatatactt catgacttca agcgcaaata
      781 caaggaagct gccgccctgc acctgaacag actgaaacaa atgaatttcc cggtcgactc
      841 cgaagaactg gccgcggccg aaaaagaatg gttcgaagga atcgactgcc taaagcaatt
      901 taaactgatc gacagtgagc atttcctgaa caaaaaaatc ggtgaaggca aaaagatttt
      961 atgcgaaggt gcacaaggca ccatgctcga tattgatttc gggtcttatc cctttgtgac
     1021 ctcgtccaat acggtttgct ccggagcctg caccggcctg ggcgtagctc ctcgctacat
     1081 cgaccgggtg tttggtattt tcaaagctta ttgtacacgg gtgggcagcg gccctttccc
     1141 cacagagctc ttcgacgaaa acggtaaact gatgcgcgat cggggcaacg aatacggctc
     1201 ggtaacgggt cgtccgcgcc gttgcggctg gatagacctg gtgactctgc gttatgccgt
     1261 aatgataaac ggagtgaccg acctgattat gatgaaaagc gacgtactcg acagcttcga
     1321 taccatcaag gcctgcgtag cctacaggat tggcggcaaa gaaaccgaaa acctgccttt
     1381 cgatattcag ggaaaactgg aaccggtatt tactgaactc ccgggatgga aaacggacat
     1441 gaccaaaatg cggtccgaag acgaatttcc cgaagaattc aatcaatacc tgagttttct
     1501 cgaagacgag ttgggtgttc gcatcaaata tgtttcggta ggtcccgacc gggctcaaac
     1561 catagaacgt tattaattat gatgatatat gtgattttta taggctttgc cctccttagc
     1621 tggatagtgc aaagcgtatt gaaaaataaa tttgataaat acagcaaaat agcccttccc
     1681 tcaggcctga gcggcaagga ggtagccgag aagatgctgc atgaacacgg catttacgat
     1741 gtcaaagtgt ctcatgtgca gggtcagctc accgaccatt acaatccgct gaccaaaacc
     1801 atcaacctga gcgaggcggt ttataacagc aacagtattg ctgccgcttc ggtggctgct
     1861 cacgaatgcg gacacgccgt acagcacgcc accgcctatt cttttttgac catgcgctcc
     1921 aaactggtgc cggtggtaaa ttttgctgcc ggatggatgc attgggtgtt gctggccggc
     1981 atattactag tacaaagctt tccctatctg ttgcttttcg gaatagtgct tttcgggctg
     2041 accaccttgt ttagttttat tacactgccg gtagaaatca atgccagctc cagagcggtg
     2101 acatggctca acaaaacggg tatcaccaac gtatcgactc atccaatggc ggttgatgcg
     2161 cttaaatggg ctgcctatac ctatgtgatt gccgctctga gttcgttggc aacgctgatt
     2221 tattatattc tgatttttct tggaggaaga aatcgaaact aaggagttat ggctattcaa
     2281 aaaccttcca tccccaaggg tacccgcgat tttacacccg aggagacagc caaacgcaat
     2341 tatattttca ataccatacg ttccgctttc gaaagctacg gctttcaggc catcgaaacc
     2401 cctgccttgg agcagttgtc cacgctgatg ggcaaatacg gggacgaagg cgataaatta
     2461 ttgttcaaga ttctgaattc gggcgacttt gccggcaaac tcagcgacga ggaattgctg
     2521 agcagagaaa ccggccggct gacccggctt atttcggaga agggactgcg ttacgacctg
     2581 acagtgcctt ttgcccgcta cgttgtgatg caccgcaacg aaattgtctt tcctttcaaa
     2641 cgttaccaga ttcagccggt ttggcgggcc gaccgtccgc aaaaagggcg ttaccgggaa
     2701 ttttttcaat gcgacgccga tgttgtgggc agcgattccc tgctcaacga ggtagaactg
     2761 ctgcaattgc tcgatacggt gttcaacgat ttgggcatca gggtttgcat caaactcaac
     2821 aaccgtaaga ttctggccgg gctggctcaa atactgggac aggaagaacg cctcacagac
     2881 attaccgtag ccatagacaa actcgacaaa atcggtttgg acaatgtccg tgcggaattg
     2941 atcgaaaaag gcatttccgc gcaaaccgtc agagaattgg agccggtgct tttgttgcag
     3001 ggcagtaaca gcgaaaaaac gactgcactc cgtcggtacc tggctgcaag caaacaagga
     3061 ttgcaaggca tcgaagaact cgactatatt ctgaagcagg ctgagaagct cctgaaaaat
     3121 gccaagctgg aactggacct ctctctggcc agaggcttga actactatac cggctgcatc
     3181 atcgaagtaa aagccctcga tgccgaaatg ggcagcattg ccggaggcgg ccgttacgat
     3241 aacctgaccg gggtcttcgg cctgcctggg gtttcgggtg taggagtttc cttcggggcc
     3301 gaccgcattt acgatgtgct gactcaactg actcttttcc ccgcaacctc tgtattgacc
     3361 actcagttac ttattaccaa cttcggagag gaagaaacgg cctggtgcct gcctttggcc
     3421 gccgaactgc gcgaaaatgg ttttagagtg gaggtttatc ccgatgccga caaaataaaa
     3481 aaacagctca gctacgccga cagcaaaggc attcctttcg tgctgatggc cggagccgac
     3541 gaaataaaac aaaacaaggt tacactaaaa aatatgcata ccgggcaaca gaatttgatg
     3601 tccccgcaag acttgctccg gtttttaaag accacaaccc tatgacagca acagaattaa
     3661 gcgaacagga actttttcgt cgccagagct tgcaagcttt gcgcgatatg ggtatcgatc
     3721 cctatccggc cgagatgtat ccaacttctg ccttttcgga cgaaatcaaa gccaacttca
     3781 aggacgaaga ggaacccaaa cgtgaagtct gtattgccgg gcgcattatg tcacgccgca
     3841 ttatgggcaa agcttcgttt atggagctca aagattcaca gggacgcatc caggtttatg
     3901 tgagccgcga cgagatttgt ccggacgaag acaaggaact ttacaacact gtgtttaaaa
     3961 aactgctgga catcggcgac tttgtgggca taaaaggtta cgtgtttcgc acccagatgg
     4021 gagaaatttc cgttcatgcc cgtgagctga aggtgctggc caaaaccctg cgccccctgc
     4081 ccatagtcaa aaccaaagac ggggtggtgt acgatgcttt cagcgatccg gaacttcgct
     4141 accgtcaacg ctgtgtagat ctggtggtaa acgaatgggt aaaagatatt ttcatcaaac
     4201 gtacccgcat tttcaattcc atgcgtaatt ttttcaacga aagaggttac ctggaagtag
     4261 atactcccgt gttgcagaac attcccggcg gagcagccgc ccggcctttt gtgacgcatc
     4321 acaacgcgct ggacattccg ctctacctta gagtggccaa tgaactctac ctgaaacgac
     4381 tgatagtggg tggtttcgaa ggcgtttatg aattctcgcg caattttcgc aacgagggta
     4441 tggaccgcag ccataatccc gaatttacgt gcatggaaat ctatgtgtcg tacaaagatt
     4501 ataattggat gatggagttt accgaacaga tgctggaacg catttgcctg gaggtgttgg
     4561 gcaccacaga ggttaaggtg ggagataata ccttgtcgtt caaggctccc taccaacgca
     4621 taagtatgat cgaagccatt caaaagtatg ccggtgtgga tattagcggt atggacgagg
     4681 atcaactgag gcaggtttgt aaaaaactcc atattgccga agacaaaacc atgggcaagg
     4741 gcaagctgat cgacgaaatc ttcggaacta cctgcgaaaa caagctgatt cagccgactt
     4801 tcgttaccga ttaccctata gaaatgtcgc cgctcaccaa gaaacaccgc aataatcccc
     4861 aactgacgga acgtttcgaa ctgatagcga acggcaagga actggccaac gcctattcgg
     4921 agttgaacga tcccatagac cagcgcgcac gtttcgaaga acaattgcgc ctgagcgaaa
     4981 agggcgacga cgaagccatg tttattgatc acgactttat ccgtgccttg gaatacggca
     5041 tgccacccac ttcggggatg ggtatcggca tggatcgctt agtgatgatg cttaccggac
     5101 agagttctat tcaggaagtt ctgttcttcc cccaaatgcg gcccgaaaaa ccggctccca
     5161 aaacagagcc gaaagctcag gtttaacaaa aaacaaaaaa ccatatggaa tttcccgata
     5221 gaatgtgtgt aatgggtgga ggcagttggg caacggctat tgccaagatg ctgctgatga
     5281 acccccaggc caggatcaac tggtatatgc gcagggcttc tcagataaga gatttcaaac
     5341 gtttcggaca caacaattcg tatctgacca ctattccctt tgacataaac cgtatccgtt
     5401 tctatagcga catcaacgtg gctgtaaaaa actccgatac tttggtactg gccacgccct
     5461 cgccttacct caagcttcac cttaaaaaat tacaacagga cgtcagcgat aaatttattc
     5521 tttcgggcat caaaggtata gtgccggacg agaacatgtt ggtttcgcaa tatttcaaca
     5581 aggtttacaa tgttccccac gaaaacattg ctaccatagc cgggccctgc catgccgaag
     5641 aagtggcgct ggaacacctc tcttacctaa ccattgcctg cagcgacaag gaaaaggcca
     5701 gggcaatagc ccgcagcttt gaatgtaaat ttgtgcgcac caccattacc gatgatgtat
     5761 tcggcataga atacgcctcg acactcaaga atatctacgc catagccgca ggtatctgcc
     5821 acggacttaa atacggagac aacttccagg ccgtactgct ttcgaatgcc attcaggaaa
     5881 cgaaccgctt tacccaggcg gcctttccgc aaaaccgaaa aatttgcgaa tcggtctacc
     5941 tgggcgactt gctggtgacg gcatactcta aattcagccg caaccgcact tttggtacca
     6001 tgattggtaa gggttattcg gtaaaaacgg cgcatatgga aatggaaatg attgccgaag
     6061 gctattatgc caccaaatgc atcaaagaaa tcaacgaaga attgtatcac gtcaatatgc
     6121 ctatattgga tgcggtgtat aatgtgctgt acgaacgtat ttctcccagt atcgaaatta
     6181 aattgctgag cgagtcactc cgttaatact ccgttaacag aaaatgctta tcttgagagc
     6241 ggtcttttga tataatcact atttaaacca tataacacat gaaacaactt gaaataaatt
     6301 taaccaaggt ttacgacctg gttcctcaaa gcgaaataac ggctttgcgc cctgcctgtc
     6361 aaaaggctat ggatttgttg aacaacggca aaggtaaagg cagcgacttt ttgggctggc
     6421 tgcatctgcc ctccctaacg gacgaaaaat tattgtcgga cattcagaaa accgccgacc
     6481 tcctgcgcga aaaatgcgag gtagtggtgg tggccggtat cggaggcagt tacttggggg
     6541 ccaaggctgt tatcgatgcg ctaaataaca gttttgactg gctcaaaact aaacgggaaa
     6601 atccggtagt ggtttacgcc ggccataaca tcagcgaaga ttacctcttt gaattgagcc
     6661 gctttctcga agacaaacgc tttggcatca tcaatatttc caaatcgggc actaccaccg
     6721 aaactgccct ggcttttcgc ctgctcaaga ccaagctcga aaaacaagtg ggcaaagaag
     6781 cagcccgcga acacatagtg gccataaccg atgcttccaa gggagccttg cgccaactgg
     6841 cccaacagga gggctacaag acttttgtta ttcccgacag cgtaggcgga cgctattccg
     6901 tacttactcc ggtaggcctg ctgcccattg cagtggccgg tcacaatatt cgtgaattgc
     6961 tggccggagc cctggctatg gaaaaacgca gcgaaacggc ttttgagcaa aatccggctg
     7021 ctctctacgc cgctgctcgc caggctttgt acaacaaggg caagaaaatc gaaatactgg
     7081 ccaattacca tcccaagctg cattttgttg ccgaatggtg gaagcaactc tacggcgaaa
     7141 gcgaaggcaa ggagcacaaa ggactgttcc cggcagccgt cgacctgacc accgacctac
     7201 attccatggg ccaatggata caggaaggtg aacgcactat ttttgagacg gtcatttcca
     7261 ttgtctctcc caatcgggaa gtagtaattc ccacagatac cggcaactta gacggcctta
     7321 actacctggc cggcaaaagg gtggatcagg taaacaaaat ggcggaactg ggcactcaga
     7381 tcgctcacgt ggacggaggg gtacccaaca tcaaaattga actgccggaa ttgaacgaat
     7441 atcacctggg tgaattgctc tacttcttcg aaaaagcctg tggaataagc ggttacatga
     7501 tgggcatcaa tcctttcgac cagccgggag tagaggccta caaaaaaaat atgtttgcct
     7561 tgttgcaaaa accgggctac gaagccgagt cggccgcaat acgttcccgc ttatgactaa
     7621 cacgaccatt ccaaaggccg ttgagaactt tctggctaaa caagggctga cggcattaga
     7681 cctcaaagcc gtgttgatcg acatggacgg agtattattc gattccatgc ctattcatgc
     7741 ctacacctgg aacaaaacca tcgccgaaga aggattgcac atgagcgaag aagaggccta
     7801 cctacacgaa ggccgcacgg gacgatcgac catagaaatc gttttccgca aacacttaca
     7861 tcgagatcct actgaggaag aagtcaagcg catctacggt aaaaaagcag gcttgttcga
     7921 ttcaatgccc gaagccccgg tgatggaagg agctcccgct ctgcttgata ctatcgcctc
     7981 ccagggcctg atccgcgtcc tggttaccgg ttcgggacaa aaactgctcc taaaccgtgt
     8041 gaaccaccat ttcccgggac atttcagctc tgaccttatg gtaaccgctt tcgatgttac
     8101 gcggggcaag cctcatcccg aacctttcct gaagggaatg caaaaggcag gtgcccaggc
     8161 tcatcaggct ctggtgatcg aaaatgcacc tttgggggtg gaagccgcca aggccgccgg
     8221 gctttttacc attgccgtca acacaggccc gctgaacgac acccacctga acaaggctga
     8281 cttgattttt cataacctga acgaactgat ccttgcctgg cctgaactga tgcggagttg
     8341 cagggaaatt tccttgcccg gacacaactg ccgaaaaccg taaaaagggc tctgcaacat
     8401 tcgctgagat gaatacaaaa cagaagattt ttctttgcct gtggttgttc ttgccctttt
     8461 tattgggttg ctgcaggcag cctgccccca caaaagaaac acacagaact gtgctggtct
     8521 atatggtggc ggataacaat ttgaacgtct atgccgttca aaacataaac tcgatgaagc
     8581 aaaacctcaa tgccgatgcc atggaaacgg gcaggttatt gattttttat gtgcctcctt
     8641 tttccgacac tcagcaattg ctcgaaatta aaatcaatac tttaaccggc caaggcgaca
     8701 ccacagtgtt gcacgaatac catttcgata atacgctcga aaaaaacgcc atgcaacagg
     8761 tggtccgcga tgccaaaaaa ctggctccgg ccaaggccta cggcctggtg ttgtggtcgc
     8821 acgccatggg ctggcatcca tccacttcgg cgctggatcc cggcagcatc gatccaccga
     8881 ccctgcggtc tgcagccgaa aacattaaag acgaagaatt gatcggctgg tttggccagg
     8941 acggttacta tcatatggag atacctgtac ttgcctcagc cctcgaagat cagggactgg
     9001 aatttatact ttttgacgcc tgttttaccg cctcggtaga aatgctttat gatttaaggc
     9061 attgcgccga ctacctgatc ggttcgccgg ccgaggtaat gggagcgggt tatccttaca
     9121 aggattttgt caagttggtt tttagggagg acctgagtac ggaagctctt tgcaggcaac
     9181 tttgtcaggc ctatatgacg tcttatagag ccaatgctac ccatccgtcg gccagcacgg
     9241 tgctggttaa actcagcgaa atggactcac tggcggtttg cacccgggct attttcgaag
     9301 ccgatcccat accggtgtcc agcattgatt tagagggtat tcagcattac gaactaatga
     9361 atccgcattt gttttacgac ctggacggct acctggcaac cgtttcgcgc tacccccttt
     9421 tttacaacgc ctttaagaat cagctcaacc ggaccgtact ttacaaagat tgtaccgatc
     9481 aaatctattc catctacagt agttcgcgtt ggttcgatgt tggcagttat tcgggcctga
     9541 gcgcctatat tccccgctac gacctgccct acagtcaaaa aataaccaac ctcaaccagg
     9601 cctattttca aactgcatgg gctcaggcaa cggggcaaac cgctccccga taaccactca
     9661 cgaaattttc ccctgctttt gcggctttcg cttaattgaa tacatttgca ggagttctca
     9721 taatccttaa tcaacactta tgaatcttaa atttgtattc ggtcttgcac tttgcggctt
     9781 ggtgctgtcc tgtacccgga ccgtgagcat cccaaccaaa gatcaggtgg gagctgccgt
     9841 gctccccggc gaaataaagc cggtaaaggc tccttttgag atgccggtat tcaaaaaacc
     9901 cgtcttcccc gatgttgaaa tcaacattgc agaccgggga gccaaagccg gtgaaaaaat
     9961 caccggtata gtccagagcg ctatcgatga gctaagtgct aaaggcggag gtaaagtcgt
    10021 ggtacccgga ggaacctggc aaagcggacg ccttatcctc aaaagcaatg ttaacctgca
    10081 ttttgccgaa ggggccgaag tctactttag cggtgaggtc gaagattacc aacctgccgt
    10141 attcacccgt aacgaaggcg tggaaatgat gtcgctgggt gccctcattt atgcctataa
    10201 acaagacaat attgccgtta cgggcaaggg taaacttatc ggaccaccta tggattgtgc
    10261 catccgtgaa caggttctta aatcgggcgt tatcgaaaac gttatttcgg ccgatacacc
    10321 cgtcgaagag agaatttaca ccggactggg cggcagcccc gtgctcctgc ctatgtttat
    10381 ttcgcccact tcgtgtacca atgtgtacat cgaaggagtg accctcgaaa gaaccgcttt
    10441 ctggaacgta gtgcccgttt attgcgacgg agtagttatt cggggcatca cggtcaattc
    10501 cgtaggcatt ccccggggcg acggcatcga cgtggaatcg agccgcaatg tgttgatcga
    10561 atattccacc ctgaattgcg gcgacgactg ttttactatg aaagccggcc gttgcgaaga
    10621 cggtatgcgg gtgaataaac caaccgaaaa cgtggtggtc cgttactgcc tggccaaaaa
    10681 aggacatgga ggcattacct gtggcagcga aaccgccggc atgattcgca atctttacgt
    10741 gcacgactgc gtctttgacg aaaccggggc gggcatccgc ttcaaaaccc gtcgaaaccg
    10801 gggcggcgga ggcgaaaata tcgtttacga gcgcatccgt atgaacctta cgggcaacgc
    10861 ctttgtgtgg gatatgctgg gctcccgtac ctacgtgggc ctgctggccg accgcctgcc
    10921 ggtgagggaa ctgacgccgt tgactccctc ctacaaaaac atcaccgcca aagacatcat
    10981 agtcgaaaga tcttctcagt ttatcaagat caccggcata cccgaaacgc ccttgaccaa
    11041 tctccgtctg gaaaacgtca aggccgaaaa ctgcgaacgt ctgatccaac tccgggatgt
    11101 ggatcaaatt catttgaaaa acgtgcaagt aagctccgac gacaacaaca tcaacgtatt
    11161 ggacggccgc aacattctct tcgaaaaagt gactttcagc aaccccgaag gcaacatcgt
    11221 ctgcctgacc gaaggcgaac tctgccgcaa catccgcttc gaagagtgca gccccgggaa
    11281 gccggaaaat tgggaaggga cggttttgga ataatctcgg aatccacgga acccaactat
    11341 gtaaaacatc aaaaagctaa cagttgtaat acaggctgtt agcttttttg tttgtaatcc
    11401 cgtggggagt cgaaccccaa tcgatggaac cggaatccat tattctatcc attgaactac
    11461 gggatctcac ttttcggggg acaaagatag cgttaagtga gagcaaaagc aaaattt
//
LOCUS       DAOSTD010000059        11425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_5603_length_11425_cov_14.084521, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000059 DAOSTD010000000
VERSION     DAOSTD010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11425
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5603_length_11425_cov_14.084521"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PLI37_05205"
     CDS             complement(<1..845)
                     /locus_tag="PLI37_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58438.1"
                     /translation="MKKTTFITLALVLISANLFAQAVSDTTFHYRDKTIQVKDSLDEV
                     RVKVSKNGSIGYVPVYEGIFTHEKSVETYTVGMDFNINTPFGPLLGKDGEKPRMRTHW
                     QGVGAGFSFLMNSDLAYRPFMSGEITLNALEISTTFLDRFGLVSGVGVVYRDYALPKN
                     LELQQSNGVASFQEMGRDLYEINSLKQLELVFSLLFEWQPKLNLKHRLFLSGGILCSD
                     IGDRAGMLVTKEKNGEYILKALNVRHNNIDVFGQIGYRDLGLYVKYTPNSIFQSQKGP
                     NFSQLS"
     gene            complement(939..1391)
                     /locus_tag="PLI37_05210"
     CDS             complement(939..1391)
                     /locus_tag="PLI37_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58439.1"
                     /translation="MKNMSKNLILTLLLAIGFLSGMNAQSDLEINKVFNQYGRQKGSV
                     LVEMSREMLEGYDFTYYKSIVTTNNQEAADLARRCLEIDQKDAKKVKQVVSNGVLSSI
                     YLQLPQKGEYFRLVLFNETTTPERKMTLIYIESEKDSEEILKIVLKKR"
     gene            complement(1410..1883)
                     /locus_tag="PLI37_05215"
     CDS             complement(1410..1883)
                     /locus_tag="PLI37_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58440.1"
                     /translation="MKITKRNNISLQEAEKLIERYYEGETSVAEENLLCEYLSGKNVP
                     ARFEAEKAILGYFASEKKKKSVWLIPNNLKWIASAAAVAAIFLAILFSNQPSATSYAY
                     VDGKKITDKDKIFALAQTTVGNLMSGMNEVEIGLENIQSGQTIESQLDVFAGMEF"
     gene            complement(1932..2435)
                     /locus_tag="PLI37_05220"
     CDS             complement(1932..2435)
                     /locus_tag="PLI37_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOQ58441.1"
                     /translation="MSSERFKTEILPLRQQLFRLSMKLLGDVQDAEDAVQESLLKLWH
                     IRDTLESYENPAAFATTVTKNICLDKIKLKKQTKTIDEYVVLIDSDNPQLQLERQDTN
                     KLVRLIIQGLPPLQQQIITMKDVDEYETEEIAEITGTTIEAIRTNLSRARKKVREEYL
                     RIVRNYE"
     gene            complement(2705..5140)
                     /locus_tag="PLI37_05225"
     CDS             complement(2705..5140)
                     /locus_tag="PLI37_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ58442.1"
                     /translation="MSIRKICTICLVLLTACSGFAQGFGNRQLINKGWYFNLGDLKYG
                     GAEFLDHSSWRQVQLPHDWSVEGTPSPQWASCTGYLPGGIAWYRKDLDIPSDLEGQKV
                     FIYFEGVYNNSEVFINGKWIGKRPNGYISFMYDLTPYLNFGGRNVLAVRVDHQDYADS
                     RWYTGSGIYRDVWLVYAPKTHIDLWGVSYQTEIKGKSALVEIQTEVLNEDLKEMSCTL
                     RQNIYDGKDKLMGSTQGRVIIPGGEKRKLEQNITVQNPELWDLDSPHLYRLETTLLHN
                     NKVLDQTDTRVGIRSIRFDPNQGFSLNGKSLKLKGICMHHDAGCLGAAVDREVWRRRL
                     IQLKSLGCNAIRTSHNPQATCVYELCDELGLLVNNEAFDEWEYPKKKWIEGWNVGTPG
                     FQGSALYFREWCKRDLADMVRRDRNHPSVIMWSIGNEVDYPNDPYSHPVLDKEGIGQQ
                     HVRGYLPGQPRAERLGEIAAELAAVVKQYDTSRPVTAGLAGAVMSNYTTYPEALDVVG
                     YNYTENRYEEDHKTYPDRVLYGSENRHGLEDWKAVTSKDYIMGQFLWTGFDYLGESGR
                     WPSRGFTTGLIDLAGNIKPRGYFRRALWSESPVIYIGTYPSGGGSRRSTRQWLSTDAV
                     PLWNYEEGRQIRVVAYTNCAEAELKLNGKTVGERKPYDEQTAIIYWDIPYEAGELEVL
                     GYESNAATASASDKIVTSGSPYAIKANFVEGSNTNFLQLEIEVVDQEGNLVYQADNEI
                     LCYVAGPASLLGLESGSNNVSDNYRDNRQRCQNGRLLAYIRLSEEVREEVRITLSSPL
                     LKSLSLTIPAGNQ"
     assembly_gap    5299..5398
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5399..7590)
                     /locus_tag="PLI37_05230"
     CDS             complement(<5399..7590)
                     /locus_tag="PLI37_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58443.1"
                     /translation="MKKSSLITLIALAAVTIFLSWWIYVPSNSFVYIYSSLNQEDTIV
                     FEGDHILYNNEKIVLGEKAFFVDGNLPNRVVKRHPFVYNSFNEAMKDIREGSPEEAMT
                     LYIAPYVYWIDNPDDPSERVGENGGIPYGLVINESNLHFYGLSKDPQHVVLACNRGQT
                     QGAVGNFTMFRFNGDNIRCENLTMGNYCNVDLVYPLKTSLNRTKRSSAITQAQLALCN
                     NSDKIFASNCAFISRLNTCPFVGSKRAFFVDCRFESTDDALCGNGVYLNCDLDFYSSK
                     PFWSTHGTGAAFLNCDFNVITRNAQYLTKVGSQVALVDCRFKNNGDSLYLGWTQYPKD
                     HMRCYQYNVSLNGRAVLFQSDRPHLTVDMLDQEVLKAYRFEYEDKLIYNTYNLLRGDD
                     DWDPQNIKDIVAAASQQDDFDYSTVPVQLSVIPQFVEVQTGKKADTLFFAVKRFGDIP
                     VEETVKWSINSEDAPYLYIRKLKNGNCLLTGSNRGDQIRTVVVEARHSSGLRGASVVR
                     VLPNILPAPIFTKKPVLSAPDQGVIKLSYSLNLQHRADHSLITWYRCKDAQGSEAIPV
                     AVSRLNQPEYRYTLSAGDVGYYLQAKIEPKHIRSHPGAAFTVCSAEPVTKEDVLNAQI
                     ITTDFQNFPADTQKQILPGFWTVDAFKPADTEAYHWQANSEGAWFYGTAEGGANGTGF
                     LQGQRGARLLYTPVDGNYGNMEVNVIADPCKTAGQGFGSATGQYMDIY"
     gene            7812..>10245
                     /locus_tag="PLI37_05235"
     CDS             7812..>10245
                     /locus_tag="PLI37_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOQ58444.1"
                     /translation="MKRQTFFRTSLIGLLFFLNFNLFAKLNNYPVPDRDTVPAFPGAE
                     GAGKFTSGGAGGQVYIVNKLDDDGSEGTLRWALEQEGRRTVVFAVDGTISLDSHLVVT
                     NGDLTIAGQTAPGQGICLKNYPLLISADNVIVRYIRSRMGDERRTQDDAMGAMRQKNI
                     IVDHCSMSWGTDECASFYNNQNFTLQWCIISESLANSIHQKGEHGYGGIWGGQPASFH
                     HNLIAHHTSRTPRFNGSRQTRRPQDEKVDFRNNVIYNWGPIDGGYAGEGGFYNLVNNY
                     YKPAYATVQNTKLVNRIFAPWVGRVSPEYPRGETGRFYVSGNYFDDGSPALDQKHKSL
                     LAAVNRDNWLGIHPNEDFKGELNELRLDKPVETAEINTQNAREAYLAVLAGAGASLHR
                     DKIDRRIVEETRTGTCINYLSGGTGIVDSQSQLGGWPELKTAPAPADSDADGMPDEWE
                     KLKGLDPNNPADASLYQLSPSYTNLEVYMNSLVCRPWEDRDLSVDERIESLVKEMSLE
                     EKCGQLVNGAPGIERLGILPYDWWNEALHGVGRNGRATVFPQAIGLAATFDEDLIFRV
                     ASAISDEARAKFNASLAMERYEQYSGLTFWTPNINIFRDPRWGRGQETYGEDPYLTSR
                     LGTAFVRGLQGDNPDYLKTAACAKHYAVHSGPEALRHEFDAHPSARDFYETYLPAFET
                     LVKEAKVEAVMGAYNRVYGLPACGSGLLLDTILRQQWGFEGHIVSDCGAIRDFYTTHK
                     VVQTPEQAAAMAANVGLNVNCGSVYRYLGAAIEQGLVTETTIDNLIRPLLRTKIKLGF
                     FDPSWANPYNSIG"
     assembly_gap    10246..10345
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10346..11176
                     /locus_tag="PLI37_05240"
     CDS             <10346..11176
                     /locus_tag="PLI37_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOQ58445.1"
                     /translation="DAKKADAIVVVLGITPLKEGEEGESIASAHKGDRPDLNLPENQV
                     NYLKKLRHNNNKPIIVVLTGGSPITMPEVTELADAILFAWYPGQEGGNALGDILFGDV
                     NPSGRLPITFPKSVDQLPPYEDYSMKERTYRYMTQDPLYPFGYGLSYTTFAYKDARLE
                     SASVKRGQSLRMEIRVENTGNRPGEEVVQFYLSKPGAGSSGPFSSLIGIRRVKLEPGQ
                     SQLLEFEISPDAMTSIDDKGKKQVLKGDYTLIVSGAAPVKRSAELGVDNRIDLKFRLK
                     "
BASE COUNT         2880 a         2588 c         2553 g         3204 t
ORIGIN      
        1 gataattggc taaaatttgg tcctttttga gattgaaaaa tgctgtttgg tgtgtatttc
       61 acatataaac ccaaatctct gtaaccgatt tgtccgaaaa catctatatt attgtgtctg
      121 acattaagtg ctttcaaaat atactctcca tttttctctt ttgtaacaag catccctgct
      181 ctatctccga tatcactaca aaggataccg ccggatagga ataacctatg ctttaagttt
      241 aatttcggtt gccattcaaa cagaagagaa aaaaccaatt ccagttgttt cagactatta
      301 atttcatata aatctctccc catttcctga aaactagcaa ccccgttact ttgttgcagt
      361 tccaaatttt tcggtaatgc atagtcgcga tagacaaccc ccacccccga gacaaggcca
      421 aaccgatcta aaaaggtcgt tgaaatttcc aatgcattta gtgttatttc accggacata
      481 aaaggtctgt aagctaaatc gctattcatt agaaatgaaa atccggctcc aactccctgc
      541 cagtgggttc gcattctcgg tttttctcca tctttaccta ataaaggacc aaacggtgta
      601 ttaatattaa aatccatacc aaccgtataa gtctctacac ttttctcatg ggtaaatatg
      661 ccctcataga caggaacata gccaattgaa ccattttttg acacttttac tctcacttcg
      721 tctaaactgt cttttacctg aatagtttta tctcgataat gaaaggtcgt atccgaaaca
      781 gcttgtgcaa ataaattagc cgaaattagc acaagggcca atgtaatgaa tgttgttttt
      841 ttcattgttt tcatttttaa ttaagtgttt gtatttactt gtatttaaat gattattcct
      901 atatcttttt gtttttcagt cttttattgt ttcttcagtt atctcttttt taaaactatt
      961 ttcaagatct cttccgaatc tttctccgat tcgatataga ttaatgtcat ttttcgctcg
     1021 ggtgttgtgg tttcattgaa taaaaccaat cggaaatatt cccctttttg aggtagttga
     1081 agataaattg aactaagaac gccgtttgaa accacttgct tcactttttt tgcatctttt
     1141 tggtcgattt ccaagcagcg acgagctaaa tcagcagctt cctggttgtt ggttgttact
     1201 atgctcttat aataggtaaa gtcataacct tccagcatct ctctactcat ttccacgagt
     1261 actgagccct tttgtctgcc gtactgattg aaaaccttat taatttccaa atcgctctga
     1321 gcattcatac cggataaaaa tccaatggct aatagcaacg taaggattaa atttttactc
     1381 atatttttca tgtctattta gttttcttgt taaaactcca ttcctgcaaa aacatctaat
     1441 tgactctcta tcgtttgacc ggattgtata ttttccaatc ctatctccac ctcattcatt
     1501 cctgacatta aatttccgac ggttgtttgt gccaaggcaa aaattttatc tttatcggta
     1561 attttttttc cgtccacgta agcatagctc gttgcagaag gctgattact gaacaaaatg
     1621 gccaggaata tggctgccac tgctgcagcc gatgctatcc acttcaagtt attagggata
     1681 agccaaacgc ttttcttctt tttttcacta gcaaaatagc ctaaaatggc tttttcagct
     1741 tcaaaccggg ccggtacatt ttttccggaa agatattcgc aaagtaaatt ctcttccgct
     1801 actgaagttt caccttcgta gtacctttca atcagctttt cagcttcttg caaggaaata
     1861 ttatttcgtt ttgttatttt cattttaata ataagcttaa atattcacca acacttaaaa
     1921 atcagaactt tctattcata attccgtact atccttaaat attcctctcg tactttcttt
     1981 cttgctctcg acagatttgt tcttatagcc tcaattgttg ttcccgttat ctcggctatc
     2041 tcctccgtct catactcatc tacatctttc atcgtaatta tctgctgttg aagcggcggt
     2101 aagccctgaa taatcaaccg tacaagcttg ttagtatcct gtctttccag ctgcaattgc
     2161 ggattatcac tatcaattag tacaacatat tcgtcaatcg ttttagtttg ttttttgagc
     2221 ttgattttat ccaagcaaat attttttgtt accgttgtag caaaagcagc cggattttcg
     2281 taagattcta acgtgtcacg tatatgccat agcttgagta gcgactcctg cacagcatcc
     2341 tccgcatctt gcacatctcc aagcaatttc atcgacaagc ggaaaagctg ttgacgcaat
     2401 ggcaatattt ctgttttaaa tcgttccgag ctcatttgct ttactgacga atcaagtcga
     2461 aaaacattac atcaaaagtt gatttttttt gaagatgttt ttcagaggtt agaaaatagc
     2521 gtgaactcta ctaagaaata caaatgattc atagtagtca aggcaggatc gagaaaactc
     2581 ggggagcacc gtagacaagg gacagagagc cccggaaaga catgaaaagc ttccggtagc
     2641 aatacaaaaa agaagtgcgg gacagaatga aatataacta attcagttca aatagcttgt
     2701 ttatttattg attaccggcg ggtatagtaa ggctcagact ttttaataag ggtgagctta
     2761 gcgtgatacg gacctcttcc cttacttctt cgcttaggcg gatgtaggct agcaggcggc
     2821 cgttctggca gcgctggcgg ttgtcgcggt agttgtcgct tacgttgttg ctgccgcttt
     2881 cgagccccaa caagctagcc ggaccagcca cataacaaag gatttcgttg tcggcctgat
     2941 aaaccagatt cccctcttgg tcaacgactt ctatttccaa ttgcaaaaag ttggtattgc
     3001 ttccttcgac aaagttggcc ttgatggcat agggcgatcc gctggtgacg attttgtcgc
     3061 tggcagatgc tgtggcggca ttgctttcat aacccagcac ttcgagttcg ccggcttcgt
     3121 atggaatatc ccaataaatg atggcggttt gctcgtcgta gggtttgcgt tctccaactg
     3181 tcttgccgtt taatttgagt tcggcttcgg cgcaattggt ataggccacc acgcgtattt
     3241 gccggccttc ttcgtaattc cagagcggta cagcatcggt cgacagccat tgtctggtgc
     3301 ttcgcctgct accgcctccg ctgggatagg tgccgatata aataaccggc gattcgctcc
     3361 agagtgctct tctaaaataa cctctgggtt tgatattgcc ggccaggtcg atcaaaccgg
     3421 tggtaaatcc cctcgaaggc catctgcccg attctcccag ataatcgaag cctgtccaaa
     3481 gaaattgccc catgatatag tctttgctcg tgacggcttt ccagtcttcg agtccgtggc
     3541 ggttttcact gccgtagaga acgcggtcgg gataggtttt atgatcctcc tcgtagcgat
     3601 tctcggtata attgtaaccg accacatcga gtgcttcggg gtaggtggta tagttagaca
     3661 taacagctcc ggccaggcct gccgtaacgg gtctggaggt atcgtattgt tttactactg
     3721 ctgccaactc ggcggcaatt tctcctaagc gttcggcccg gggttggccg ggcaagtagc
     3781 ccctgacgtg ttgttgccct ataccttcct tatcgagtac gggatgtgag tagggatcgt
     3841 tgggataatc aacctcattc ccgatactcc acatgatgac actgggatga ttcctgtcgc
     3901 gcctgaccat atctgccaga tctcgcttac accattctct gaagtaaaga gcagaaccct
     3961 gaaaaccggg agtgcccaca ttccagcctt ctatccattt cttttttgga tattcccatt
     4021 catcgaaggc ttcgttgttg accaataagc ctaattcgtc gcaaagctca tagacacagg
     4081 tagcctgagg gttatggctg gtgcgtatgg cgttgcagcc cagcgatttg agttgaataa
     4141 gccttcttcg ccacacttca cggtctacgg cagctcccag gcagccggca tcgtggtgca
     4201 tgcagatacc tttgagtttt agtgatttgc cgttcagact gaaaccttgg ttgggatcaa
     4261 aacgtatgct tcgtatgccg actctggtgt cggtttgatc caaaactttg ttgttgtgca
     4321 ataatgtggt ctccaggcga tagagatggg gagaatccaa atcccagagc tcaggatttt
     4381 gtactgttat attctgttcc agcttgcgtt tttctcctcc cggtatgatc actctccctt
     4441 gggtgctgcc catcagtttg tctttgccgt cgtatatgtt ttggcggagg gtacaactca
     4501 tttctttcaa atcctcgttg agcacttcgg tttgaatctc gacgagggcg cttttccctt
     4561 ttatctccgt ttgataacta acaccccaaa gatcaatatg ggttttggga gcatacacca
     4621 gccagacatc gcggtaaata cccgaacccg tataccatct cgaatctgca tagtcttggt
     4681 gatcgactct gacagcgagc acgtttctgc ccccaaaatt aagatagggt gtcagatcgt
     4741 acataaacga aatataaccg ttagggcgtt tgcctatcca tttgccgttg ataaaaactt
     4801 cgctgttgtt gtaaacgcct tcgaaataga taaaaacttt ttgtccttcc agatcggaag
     4861 gaatgtcgag gtctttgcga taccaggcta tgcctccggg caaatagcct gtgcagctgg
     4921 cccattgcgg gctgggagtg ccctcaacgc tccagtcgtg cggcagttgc acctgtctcc
     4981 acgaagaatg atccagaaac tcggctccac cgtatttcag gtcgcctaag ttaaaatacc
     5041 agcccttgtt gatgagctgc cgattgccga agccttgtgc aaaaccggaa caggcggtca
     5101 gcaataccag acaaattgta catatcttac ggattgacat aattgtgtag atttaaggtt
     5161 gctcttgata aataatttcc attttgtgca acatagtggc accgcttcct gcagatccgg
     5221 tgtgttggat accgctgcct ccaaagttgt tttcggtgat ttccgctgtc agcttcactt
     5281 cgtgaggcaa gtcgggatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     5401 gtaaatatcc atgtattgac cggtggcgct gccaaagccc tggccggctg ttttgcaggg
     5461 gtcggcaatt acatttacct ccatattacc gtaattaccg tcgaccgggg tgtaaagcag
     5521 gcgggcacct ctctgacctt gcagaaaacc ggttccgttt gctccgcctt cggccgttcc
     5581 gtaaaaccag gccccctctg aatttgcttg ccaatggtag gcttcggtat cggccggttt
     5641 aaaggcatcg actgtccaga aaccgggcag gatttgcttt tgcgtatcgg cggggaaatt
     5701 ctgaaaatcg gtggtaataa tttgggcgtt caaaacatct tcttttgtaa ccggttcggc
     5761 gctgcaaact gtaaaggcag ccccgggatg gctccttatg tgcttgggct cgattttagc
     5821 ctgaaggtaa taaccgacat cacctgcact gagagtgtac ctatattccg gttggttcag
     5881 gcgactgacg gcaacaggaa tggcttctga gccctgggcg tctttgcagc ggtaccaggt
     5941 aataagggag tggtctgccc tgtgttgaag attcagactg taacttagct ttataacgcc
     6001 ctgatcgggg gcagacaaca caggtttttt tgtaaaaatg ggagcaggaa gaatgttcgg
     6061 taagaccctg accacagaag ctcctctaag tccggaagag tgtctggctt ccaccacaac
     6121 ggttcgtatc tgatcgcccc ggttgctccc ggtcaacagg caattaccgt ttttcagctt
     6181 gcgaatgtac aaataagggg catcttcgga gttgatagac catttgactg tttcttcgac
     6241 cgggatatcc ccaaaccgct tgacggcaaa gaaaagtgta tctgcttttt tcccggtttg
     6301 gacttcgaca aactgcggta ttacactcag ttgaacggga accgtgctat aatcgaaatc
     6361 gtcttgctga gaggccgcgg ccactatatc tttaatgttc tgcggatccc agtcgtcgtc
     6421 gccgcggagc aaattatacg tgttatagat gagtttatcc tcgtactcaa aacggtaagc
     6481 cttcaatact tcctggtcga gcatatctac agtcaggtga ggacggtcgg attgaaacag
     6541 cacagcccgg ccgtttaaac tcacattgta ttggtaacag cgcatatggt ctttggggta
     6601 ttgagtccag cccaaataga gggaatctcc gttgttttta aagcgacaat ctaccaaagc
     6661 cacttggctg cctactttgg tcaggtattg ggcgtttcgg gtaattacgt taaaatcgca
     6721 attcaaaaaa gcagcacccg tgccgtgtgt actccagaaa ggttttgaac tgtaaaaatc
     6781 gaggtcgcaa ttcaggtaga ccccgttgcc acacaaggcg tcgtcggtac tttcgaaacg
     6841 acaatcaaca aaaaaagctc ttttgctgcc tacaaaagga caggtattta atcggcttat
     6901 aaaggcacaa ttgctggcaa aaattttgtc gctgttgttg catagtgcca gttgagcctg
     6961 ggtaatggcc gaagagcgtt tcgttctgtt gagagatgtt ttgagcgggt aaaccaggtc
     7021 tacattgcaa taatttccca tagtcagatt ttcacaacga atgttatcac cgttgaaacg
     7081 aaacatggta aaattgccca cagctccctg tgtctgccct ctgttgcagg ccaaaaccac
     7141 atgttgagga tctttgctca agccgtaaaa atgcaaatta ctttcattaa taaccaggcc
     7201 gtaagggatt cctccatttt caccgactcg ttccgaagga tcgtccggat tgtctatcca
     7261 ataaacataa ggagcaatat acaaggtcat tgcttcttcg gggctgcctt cgcggatgtc
     7321 tttcattgcc tcgttgaaag aattgtacac aaagggatgg cgtttgacaa ccctgttggg
     7381 caaattgccg tcaacgaaaa aagccttctc tcccaatacg attttttcgt tgttgtagag
     7441 gatatgatcg ccttcgaaaa caatagtgtc ctcttgatta agagaggagt aaatgtatac
     7501 aaaggaattg ctgggtacat aaatccacca gcttaaaaaa atggtgacgg ctgctaacgc
     7561 gattagggtg atcaggctgg attttttcat ttttccggag ttttaaggtt tgcaaatata
     7621 gttatatatt atggtttgtt gttgaattgg gccggtttat aagctatttt tcattattga
     7681 aactattttc gatttgttcc tgaacttgat ttaataaatg tgatattttt gcctcattcc
     7741 tttatttaaa ggcaaaaaga gctatttttg tctctcaatg caaataaaag tctgaatata
     7801 aatcaataag catgaaacgc caaacttttt tccgcacaag tctgatagga ctgctttttt
     7861 ttctgaattt taatctgttt gcaaagttaa acaattatcc cgttcccgac agggataccg
     7921 tcccggcttt ccctggagcc gaaggggccg gtaaattcac ttcgggaggc gccgggggac
     7981 aggtttatat cgttaacaaa ctggacgacg acggatcgga aggaaccctt aggtgggctt
     8041 tggagcagga aggaagacga acggtggttt ttgccgtaga tggaactatt tcgttggata
     8101 gtcatctggt tgttactaac ggcgatttaa ccatagccgg acaaacggct cccggtcaag
     8161 gtatttgtct gaaaaattat cctttgctga tttctgccga taatgtgatt gttcgttata
     8221 tacgctccag aatgggagat gagaggagaa ctcaagacga tgccatgggt gctatgcgcc
     8281 aaaagaatat tattgtggat cactgctcca tgagctgggg taccgatgaa tgtgcctcct
     8341 tttacaataa tcaaaatttt accctgcaat ggtgcataat aagtgaaagc ctggccaatt
     8401 ccattcacca aaaaggagag catggttatg gcggcatttg gggcggacaa cccgcgagtt
     8461 ttcaccacaa tctgattgct caccacacca gtcgtactcc ccgttttaac ggaagccggc
     8521 aaactcgcag accgcaagac gaaaaagtgg atttccgcaa caacgtcatt tacaactggg
     8581 gaccgatcga tggcggctac gcaggtgagg gaggctttta caatctggta aacaattatt
     8641 ataaaccggc ctatgccact gtgcagaaca caaaactggt caatcgcatt tttgcaccct
     8701 gggtcggcag agtcagtccc gaatatccca ggggtgaaac cggccggttt tatgtgtcgg
     8761 gtaattattt tgacgacggt tctccggccc tggaccaaaa gcataaatct ctgctggcag
     8821 ccgtgaatcg ggacaactgg ttgggcatac atccgaacga agatttcaaa ggggagttga
     8881 acgaattaag gttggataag ccggtcgaaa cggccgaaat caatacgcaa aacgcccggg
     8941 aagcttatct ggcggtgctg gccggtgccg gcgcttctct ccatcgtgac aagatcgata
     9001 gacgtatagt ggaagaaacc cgaacaggta cttgtattaa ttacttaagt ggcggaacgg
     9061 gtatagtaga cagccaaagc caactgggcg gctggcccga actcaagaca gcgccggctc
     9121 cggcagattc cgatgcagac ggtatgcccg atgaatggga aaagcttaag gggctggatc
     9181 ccaataaccc cgcagacgcc agtctgtatc agctgagtcc gtcttatacc aatctagagg
     9241 tgtatatgaa ctccctggtt tgccggccct gggaggatag ggatttgtcg gtggacgaac
     9301 gcatcgaatc cctggtgaaa gaaatgagtc tcgaagaaaa atgcggccag ttggtcaacg
     9361 gagcgccggg tatcgaacgc ctgggcatat taccctacga ttggtggaac gaagctttac
     9421 acggtgtcgg ccgaaacggc agggctaccg tttttcctca ggccatcggt ttggcagcca
     9481 cattcgacga agacttgatt ttcagagtgg cttcggctat ttcggacgaa gcccgggcca
     9541 agttcaatgc ctctttggcc atggagcgtt acgagcaata ctccggcctg actttttgga
     9601 ctcccaacat aaatattttt cgagatcccc gctggggcag ggggcaggaa acctacggcg
     9661 aagaccctta tctgacttcg cgtttgggaa cggcttttgt gcgcggtttg cagggagaca
     9721 atcccgacta tcttaagacg gcagcctgcg ccaaacatta tgctgtacac tccggccccg
     9781 aagcattacg tcatgaattc gacgctcatc cctccgccag ggatttttac gaaacttatt
     9841 taccggcatt cgaaaccctg gtcaaggagg ccaaagtgga agctgtgatg ggagcataca
     9901 accgggtcta tggtttgccg gcctgcggca gcggcttgct tttggacacc atactcaggc
     9961 aacaatgggg tttcgaggga catatcgtgt cggattgcgg agccatcaga gatttttata
    10021 ccacacacaa ggtggtacaa actcccgaac aagcggccgc catggcggca aatgtcggtc
    10081 tcaacgttaa ttgcggttcg gtttaccggt atttgggcgc ggccatcgaa caagggcttg
    10141 taacggaaac caccattgat aatttaatcc gtcctttgtt gaggactaaa ataaaattag
    10201 gcttcttcga cccctcatgg gccaatcctt acaacagcat aggccnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnngacgc caaaaaagcc gatgccatag tcgttgtgtt
    10381 gggaattacc ccactcaaag aaggcgagga gggggaatcg atagcttctg cacacaaagg
    10441 cgatcgcccc gatttgaatt tgcccgaaaa ccaggtgaat tatttgaaaa aactgcgtca
    10501 taacaacaat aaacccatta ttgtagtcct gaccggaggc agtcccatta ccatgcccga
    10561 agtaacagag ctggccgatg ctattttgtt tgcctggtat ccgggccagg agggtggaaa
    10621 cgccttgggc gacattttat ttggagatgt aaatccttcc ggcagattgc cgattacttt
    10681 ccctaagtcg gtagatcaat tgcctcccta cgaagattat tccatgaaag aaagaaccta
    10741 tcgctatatg actcaggatc ccttgtatcc ttttggttat gggctgagtt atactacgtt
    10801 cgcttacaag gatgcccggc tggaatcagc ttccgtcaaa agaggccagt ccctgcggat
    10861 ggaaatcaga gttgaaaata caggtaaccg tccgggcgaa gaagtggtac agttttacct
    10921 tagcaaaccc ggagccggaa gcagcggccc cttcagttcc ctgatcggta tcaggcgggt
    10981 aaaactggag cccggtcaat ctcaactcct ggagtttgaa atcagccccg atgccatgac
    11041 ttcgatcgac gataagggga aaaagcaagt tctcaaagga gattataccc tgattgtatc
    11101 gggagcagct cccgtcaagc gaagtgcaga gctgggagtc gacaatcgca tagatttgaa
    11161 atttcgacta aaataaacca tgaatccact ttaccatgtt gggaggtatt cccaacttat
    11221 gtacaggtgt ttttccaagc cacaacggtc gggagtgttt gttagtcaaa cacttaaaga
    11281 aatcgatcgc ctgggtgtaa gctcactttt tattaccctg atcgtttcct tgtttatggg
    11341 ggccgtcctg accattcaaa cccaaaacaa catcgaaaat cctctgttgc ccgattatac
    11401 cgtggggctg gttacccgcg atacc
//
LOCUS       DAOSTD010000060        11407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_5610_length_11407_cov_12.702960, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000060 DAOSTD010000000
VERSION     DAOSTD010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11407
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5610_length_11407_cov_12.702960"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLI37_05245"
     CDS             <1..281
                     /locus_tag="PLI37_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58446.1"
                     /translation="IDEHDWTIVLEKDITAHVLPTIAYVNLYIERFLLPLKHYAYEKL
                     RYKQAWLFYLSERIRNSIDAKINKERRSVDPVLFVSQKRYMFFVLEKK"
     gene            complement(307..1365)
                     /locus_tag="PLI37_05250"
     CDS             complement(307..1365)
                     /locus_tag="PLI37_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOQ58447.1"
                     /translation="MKTMQAAYLPGNSTVVLKEVEIPVPGHGEVLIKMKSSTICGSDI
                     RAIYREHLGKGPEGYQNKICGHEPSGQIVKCGPGLRRFKEGDRVVVYHISGCGLCNDC
                     RRGYMISCTSPYRRAYGWQRDGGMAEYLLAEEKDLVLLPDSLTYTDGAQIACGFGTVY
                     EGLEKIGISGNDAVLVVGLGPVGLASLMLAKAMGANKLIGVDTVQERLDIAKEKGLAD
                     AVFLSDADALKKIQDLTGGNGVERAVDCSGNTYGRQLAVRATRKWGKIVLIGEGGTME
                     LNPSPDLIHDQKAVYGSWVTNIWRMEELVERIVRWNIHPEDLVTHRFKLDRVSEAFAL
                     MAEGKCGKVAVVFDEEIR"
     gene            complement(1441..2457)
                     /locus_tag="PLI37_05255"
     CDS             complement(1441..2457)
                     /locus_tag="PLI37_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ58448.1"
                     /translation="MNKEMKCVDPRLVPAVLLATGDKIPVIGMGTFGSDNYGAEEIAE
                     AVRVAIKMGYRHLDCAAVYGNEKEIGDVLRELFQEGVVKREELWITSKVWNDKHHDVK
                     GACKDSLKKLGLDYLDLYLVHWPFPNHHPPMVDVESRDPHARPYIHEEYMAVWKAMEQ
                     LADDGLVRNIGTSNMTRAKMELLLRDCRIKPVCNEMELHPHFQQPELKKYFEDQGITV
                     IGFSPIGSPNRPERDKTPEDTVPIEDPVIVEIARKHGVHPAVICIKWGAQNGQIPIPF
                     SVKPEKLFSNLLAVVQDPLSAEEMEAIREQDKNCRFIKGQVFLWPGARSWEDLWDLDG
                     IIRQ"
     gene            complement(2469..3737)
                     /gene="fucP"
                     /locus_tag="PLI37_05260"
     CDS             complement(2469..3737)
                     /gene="fucP"
                     /locus_tag="PLI37_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HOQ58449.1"
                     /translation="MTKTQVKVVTREFLLPFILVTSLFYFWGIANNMTDTLLSAFKRI
                     MSMTDVQTSLIQLSFYGSYFCFALPAALFIKRYSYKSGVILGLILYAGGAMLFYPAAQ
                     TASYGFYLFAIYVLAGGCSILETTANPYILSMGSPETATRRLNLAQAFNPLGSITGIL
                     LSQYFILSELSSADATQRAQMDPEQLRLMQTGELNAITYTYMGVGFVLFIILLIMIFS
                     RMPKNFDRDRSIQLGSTFKRLIKNKIYSWGVVAQFFYVGAQIGVWSFTIRIVMQELGI
                     NEQSAASVYLLSIICFSISRFIFTWLMRYIRPSRLLIIAAIADIVCTAAVILGGGSGM
                     FLIVMLVGVSVFMSLMFPTIYGIALDGLGEDAKIGASGLIMAILGGALLTPLQGLVSD
                     LAGINIAYSVPLICFVVVMAYGIMAQKVGK"
     gene            complement(3792..5564)
                     /gene="fucI"
                     /locus_tag="PLI37_05265"
     CDS             complement(3792..5564)
                     /gene="fucI"
                     /locus_tag="PLI37_05265"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006005 - L-fucose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOQ58450.1"
                     /translation="MTKHPKVGIRPIIDGRERGVRESLEVQTMNMAKRVAELISSTLK
                     YGDGTAVKCVIADSTIGRVPEAAACAEKFEREGVGLTISVTPCWCYGSETIDMNPHYP
                     KAIWGFNGTERPGAVYLAAAMAAHNQKGLPAFGIYGRDVQDSDDNTIPEDVKEKLLRF
                     TRAGLAVAEMRGKSYLSIGGVCMGIAGSMVNPDFFQEYLGMRNEYIDMVEVSRRIDKG
                     IYDHEEYKKAWEWTLKYCKVNEGEDFNDPQWRKTRAEKDADWEYVVKMYLVIRDLMVG
                     NPKLAELGFKEESLGHNAIVAGFQGQRQWTDYLPNCDFPEALLNSSFDWNGLRRPYIL
                     ATENDSLNGVSMLLGHLLTNTASIFSDVRTFWSPEAVKRVTGYTLEGRAAHGIIHLIN
                     SGATTLDATGQQSNKQGEPAMKPFWEISQDEVAKCLKATTWSPANCGYFRGGGYSSNF
                     LSKGGMPVTMSRINMIKGQGPVMQIAEGWTVDIQPKAYEIINNRTDKTWPTTWFAPRL
                     CNKLQFKDVYSVMNNWGANHGAINYGHIGADLITLASLLRIPVCMHNVDEGKIFRPAA
                     WSAFGMDLESSDYRACNNFGPIYK"
     gene            complement(5722..7227)
                     /locus_tag="PLI37_05270"
     CDS             complement(5722..7227)
                     /locus_tag="PLI37_05270"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOQ58451.1"
                     /translation="MIRKSLISWSLVFLACLFASCEGESSFTHNGKEEADSLSLNNSY
                     VNKWIYNGMSLAYYWSDKMIEPEDYTQSPISFFNDLLYRYHPTTAPDGDRFSFIRSDY
                     ANLLNTFSGLRSREIGFDYQLYLRSENDKAIVGQVTYVKPNTPAVDAGVQRGWWFDQI
                     GGIALNTDNYTDLLKNLPAEITLRFLNRAPQQTPENFEAYLNAMHVDTLTFAVLEKYA
                     ENPNYLDTVYQIGNKKIAYFLYNFFAFGPEDKSDRFLLETNEIFGRFKALDVDELVLD
                     LRYNSGGYVMMSTYLSSMILPALSRDKVFCYFRYNDYLQAFYNKQYGEDYFLMHFKEN
                     FFPAGSSLAVLLNNLNLERLIVLTGEYTASASEQLINGLKAYMPVILIGKTTYGKNVS
                     SLSIYEQNNPKNRWGMQPIVAKVFNSLGESDYTTGFEPDIELTELSVYDQSALGDTSE
                     LLLNAAISLILDRSVATQRSARKEIQTPVFISDETKIKGSLLPAEPDLNER"
     gene            complement(7270..9744)
                     /locus_tag="PLI37_05275"
     CDS             complement(7270..9744)
                     /locus_tag="PLI37_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOQ58452.1"
                     /translation="MHLPVSEIARLIGVQKAIIVQDTVIERLLIDSRALLVPEGCLFF
                     AIKTESNNGHKYLSDLYKRGVRNFVLSEKQPESLFDQANFLYVEDAIEALQLLGRYCR
                     KIFEGKVLGITGSNGKTIVKEWIWQLLRDNLSISRSPGSYNSRLGVPLSLSMLSEDSR
                     LGLIEAGISQVGEMQKLQNMIQPDWGLLTHIGQAHNENFDSVESLVREKLLLFKDCKR
                     VIYCQDEEPSAGILAGMLEPKRLLAWSCNDLSAELYFRKDKQKDYTQIHTLWHREEVC
                     FVLPFVDEPSIKNALHCIAFLFLLEQETDFVIDASVLGGRLKQLEPVAMRMEVKEGRN
                     GCVLIDDAYNSDLQALELALDFQSRRALAGKMRSCLILSDIFESGKSMDDLYGTVAEL
                     IKRKQIDRFIGIGYGLVTHAGFFPSSARFFLTTDDFLLSDLPDQFQDELILIKGSRQF
                     GFERISERLELKTHNTVMEVDLNALVHNLNYFRTLIKPQTKVIAMVKAEAYGHGALEV
                     SKTLQAQGCEALAVALADEGVKLRRGGIHIPILVMNPDPGIFPLCYEYKLEPEIYSFA
                     LLDAFMASAERLAVRDFPVHIKLDTGMHRLGFTEKDLPELLSKLNSRPELRVSSVFSH
                     LAGSDENAFDKYTKTQWKLFKLYTRQMSRALPYRFERHILNSAGIERFPQWQCDRVRL
                     GIGLYGVSVCSPEALQPVASLKTRILQIHEFKKGESIGYSRTVLLDRDSRIATLPVGY
                     GDGFNRHHTGGDVLVNGQRAKILGNICMDLCMVDVTDIAAHEGDTVVLFGKGLPLTEL
                     AERLSTISYEVLTSVSERVKRVYFKE"
     gene            complement(9728..10729)
                     /locus_tag="PLI37_05280"
     CDS             complement(9728..10729)
                     /locus_tag="PLI37_05280"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOQ58453.1"
                     /translation="MDFKTTLTLPKSSFRLNHSDSILCLGSCFAESVGKQLQKSGFDC
                     LNNPFGVLYNPSSVIRALRAMQENKSYSKEDIFEYKGLYGSFDFHSSFSGREAEKVLE
                     GIQTACSTSSSAFRSSTCLILSFGTAWVYELASNGLVVSNCHKLPASFFDRRRLSVGE
                     MLEQTVGFFEELFRTRPKLKVLLTVSPIRHFKDGAHENTLSKASLHLLVDELCSRFEQ
                     MVYFPAYELLMDDLRDYRFYEQDMLHPSAMAFQYIWERFGEFAFEKYTLEIIRQLEQI
                     HKAMDHKPFRPDDEEYLRFIKKNIAALHLLGKRFPELNLEKERVFFESVLHSNASTC"
     gene            10824..>11407
                     /locus_tag="PLI37_05285"
     CDS             10824..>11407
                     /locus_tag="PLI37_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOQ58454.1"
                     /translation="MMERKTSLLDEIQYPEDLKKCSVSQLTQVCEELRQFIIDALSEN
                     PGHLGASLGTVELTVALHYVLNTPNDRIVWDVGHQAYGHKILTGRKKLFYTNRKLNGI
                     SGFPNPLENEHDHFIAGHASNSISAALGMDMAAAFKKEKRVVVAVIGDGAMTGGLAFE
                     GLNNASVYPNNLLIILNDNQYAIDKNIGGVSRYLS"
BASE COUNT         3139 a         2803 c         2424 g         3041 t
ORIGIN      
        1 aaatcgacga acacgactgg accattgtgc ttgaaaaaga tattaccgcc cacgttttac
       61 ccactattgc ctatgtcaat ctgtacatag agcgtttcct cttgccttta aagcattatg
      121 cttacgaaaa actccgttac aagcaggctt ggctatttta tttgagcgaa cgtatccgca
      181 actccataga cgccaagata aacaaagagc gcagaagtgt tgatcccgtg ctctttgtta
      241 gtcaaaaacg ctatatgttc tttgtactcg agaaaaagta atttcagcga gtttccaatc
      301 ttgatctcaa cggatctcct catcaaaaac cacggccacc ttgccgcatt taccttcggc
      361 catcagggca aacgcttcgg atactcggtc aagcttgaac cgatgcgtta ccaggtcttc
      421 gggatggata ttccaacgaa caatacgctc gaccaactct tccatacgcc agatattggt
      481 aacccaggaa ccgtaaacgg ctttttgatc gtggatcagg tccggactgg gattcaactc
      541 catagtgccg ccttcgccaa tcaaaacaat tttaccccat ttacgtgtgg cacggacggc
      601 taactggcgg ccataggtat tgcccgaaca atctactgca cgctctactc cgttacctcc
      661 ggtaagatcc tgaattttct ttagcgcgtc ggcatccgac aagaagacgg catcggccag
      721 acccttttct ttagctatat ccagacgctc ctgcacggtg tctactccaa tcagcttatt
      781 ggcacccata gccttggcca gcatcagcga ggccagaccc acggggccaa gaccgactac
      841 caaaacagcg tcgttgcccg aaatgcctat tttctccaag ccttcataaa cagtgccaaa
      901 accgcaggca atttgtgccc cgtcggtata agtaagggaa tcgggcaaga gaaccaaatc
      961 cttttcttcg gccagcaggt attcggccat acctccgtcg cgctgccagc cataagctct
     1021 ccgataggga ctggtacaac tgatcatata acctctacgg caatcgttgc acaatccgca
     1081 acccgaaata tgatagacaa ccactctgtc gccctcttta aaacgacgta aacccggacc
     1141 gcatttcacg atctgtcccg agggttcgtg tccgcatatc ttgttttgat agccctcggg
     1201 gcccttgcct aaatgttctc tgtaaatagc acggatatcg ctgccacaaa tggtggatga
     1261 tttcatttta atcaggactt cgccgtgtcc gggaacgggg atttctacct ctttaagtac
     1321 cacagtactg ttgccgggca gataagctgc ttgcattgtt ttcataagcc ttacacttct
     1381 tgcttttttt atcttaattc tatataaacc gaatgctcca tcgcatgttg cattccgtgt
     1441 ttattgacgt ataatgccgt ctaaatccca gagatcttcc caggaacggg ctcccggcca
     1501 aaggaagacc tgacctttta taaaacggca atttttatct tgttcgcgga tggcttccat
     1561 ttcttcggca ctcagagggt cttgtaccac tgccaagaga ttactgaaca gtttttccgg
     1621 ttttaccgaa aaaggaatgg gaatttgtcc gttctgagcc ccccatttaa tgcatattac
     1681 tgccggatgt actccgtgtt tgcgagctat ctcaacgata accggatctt cgatgggaac
     1741 agtatcttcg ggtgttttgt ccctctccgg acggttggga gaaccaatcg gcgagaaacc
     1801 tatcacggtt atgccctgat cttcaaaata ctttttcaat tcaggctgtt gaaaatgggg
     1861 atgcaattcc atttcgttgc aaaccggttt gattctgcaa tcgcgcagca gcaattccat
     1921 tttggcccgg gtcatattgg aagtaccgat attacgcact aaaccatcgt cagcaagttg
     1981 ttccatggcc ttccatacag ccatgtattc ttcgtgaata taaggacggg catggggatc
     2041 gcgcgattcc acatcgacca tgggaggatg atgattggga aagggccagt gtaccagata
     2101 caagtccagg taatccaaac ccaatttttt caaagaatct ttacaggctc ccttgacatc
     2161 atggtgctta tcgttccata ctttggaagt aatccagagt tcttcccttt tgactacccc
     2221 ctcctgaaac aattcccgca gcacatcacc tatttctttt tcgttgccgt aaactgcagc
     2281 acagtctaaa tgcctgtaac ccattttaat agctactctg accgcctcag caatttcctc
     2341 agccccgtaa ttgtcagagc cgaaagtccc catgccgatg acaggaattt tgtcgcccgt
     2401 tgccagaagt acggcaggca ctaaacgggg atctacacat ttcatttctt tattcataaa
     2461 aatcttcttt attttccgac tttttgagcc attatgccgt aagccatcac tactacaaaa
     2521 caaataagag gtacactata agctatattg atcccggcca aatcggaaac cagtccctgt
     2581 aaaggggtca acagggctcc tccgagaatg gccatgatca atcccgaagc accgatcttg
     2641 gcatcctcgc ccaaaccatc cagggcaatg ccgtaaatgg tgggaaacat caacgacata
     2701 aacaccgaaa cccccaccaa cataacgatc aaaaacatcc cactgcctcc gccaagaatt
     2761 acggcagccg tacaaacaat atcggctatc gcagcaataa taagcaaacg tgaggggcgg
     2821 atatagcgca ttaaccaggt gaaaataaac cgggagatgc taaagcaaat gatcgacaac
     2881 aaatacacgc tggctgccga ttgttcgttg atacccaatt cctgcattac aatgcggatg
     2941 gtaaaagacc aaacaccaat ttgcgctcct acatagaaaa attgtgccac tacaccccaa
     3001 ctgtatatct tgttttttat cagtcgttta aaagtcgatc ccaattgtat ggacctgtct
     3061 ctgtcgaagt ttttgggcat acgggaaaaa atcatgatca ggagaatgat aaacaaaacg
     3121 aaacctaccc ccatataggt gtaggtgatc gcattcagtt ccccggtctg cataagcctc
     3181 aattgttcgg gatccatctg tgcgcgctgt gtggcatcgg cgctgctgag ttctgaaaga
     3241 atgaaatact gactcagcaa aattcccgtg atcgagccca gggggttgaa agcctgagcc
     3301 agattgagcc tgcgggtggc cgtctcggga gaacccatac tcaatatgta gggattagca
     3361 gtcgtttcca gaatggaaca acctccggcc aacacataaa tggcaaaaag gtaaaagcca
     3421 taactggcag tttgggcagc cggataaaac aacatcgcgc caccggcata aaggatcaaa
     3481 cccaatataa caccggattt atacgaatag cgtttaataa aaagggcggc cggcaaggca
     3541 aaacaaaaat aagaaccgta aaacgacagt tgtatcaggg atgtctgaac gtctgtcatg
     3601 ctcataatcc gtttgaaagc agacagcaac gtatctgtca tattgttggc tataccccag
     3661 aaataaaaaa gggaggttac caaaataaaa ggtaacaaga actctcttgt taccactttt
     3721 acctgagttt ttgtcatagc tgtaaggggc aaaagtaagg ggaaagcatt cttgctttcc
     3781 cccggataaa gttatttata gattggacca aaattgttgc aggcacgata atcggacgat
     3841 tcgagatcca ttccgaaagc actccaggct gccgggcgga aaatttttcc ttcgtccaca
     3901 ttatgcatgc aaaccggaat tctgagcaga gaagccaggg tgatcaaatc ggcccctata
     3961 tgtccgtaat tgatagcccc gtgattggcg ccccaattgt tcatgacact gtaaacatcc
     4021 ttgaattgca acttgttgca caaacgggga gcaaaccaag tggtcggcca ggttttatcg
     4081 gtacgattgt ttattatttc gtaggccttg ggctgaatat caacggtcca accttcggca
     4141 atttgcatta cgggaccctg tccttttatc atattgatac gggacatggt aacaggcata
     4201 ccgcctttac tcaggaaatt cgaagaatag cctccgcctc tgaaataacc gcaattggca
     4261 ggagaccagg tggtggcttt gaggcattta gctacttcgt cctggctgat ttcccaaaaa
     4321 ggtttcatgg cgggttcgcc ctgtttgttg ctttgctgtc cggtagcgtc gagagtggtt
     4381 gcaccggaat tgatcaagtg aatgatgccg tgagcagctc tgccttcgag ggtatagccc
     4441 gtaacccttt ttaccgcttc ggggctccag aatgtgcgca catccgagaa tatggaagcc
     4501 gtattggtca ataaatgacc cagcagcatg gaaacgccat tcaaggagtc gttttcggtg
     4561 gccagaatat aaggtctgcg taggccgttc caatcgaaag aactgttgag caaagcttcg
     4621 ggaaaatcac aattgggcag gtagtccgtc cactggcgtt gtccctggaa cccggctacg
     4681 atggcattgt gacccaaaga ttcttcttta aagcccagtt cggccaattt ggggttaccc
     4741 accatcaaat cgcgaataac caaatacatc ttgaccacgt attcccagtc ggcgtctttt
     4801 tcggcgcggg ttttacgcca ttgaggatcg ttaaagtctt ctccttcatt gactttgcaa
     4861 tacttaagcg tccattccca agctttcttg tattcttcgt gatcgtaaat gcctttgtcg
     4921 atacgacggc tcacctcgac catatcaatg tattcattgc gcatacccag gtattcctgg
     4981 aagaaatcgg gattcaccat agaaccggca atacccatac aaactccgcc tatggagaga
     5041 taggatttgc ctcgcatttc ggcaacagca agtccggctc tggtaaaacg cagcaatttt
     5101 tcttttacgt cttcgggaat agtgttgtcg tcggaatcct gaacatcccg tccataaata
     5161 ccaaaagcag gtaaaccttt ttgattatga gcggccatag cggctgccag ataaacggca
     5221 ccgggacgtt ccgtgccgtt aaaaccccaa atagccttgg ggtaatgggg attcatgtcg
     5281 atggtttcac ttccgtagca ccaacagggc gttacgctga tagtgagccc aacaccttcg
     5341 cgttcaaatt tttcggcgca ggctgcagct tcgggcacac ggcctatggt gctgtcagca
     5401 ataacgcatt tcacggcggt tccgtcgccg tatttcagcg tggacgaaat caattcggcc
     5461 actcgtttag ccatattcat ggtctgcact tcgaggcttt ctcgcacacc tcgttctctc
     5521 ccgtcaatta tgggacgtat tcctacttta ggatgtttag tcataatgtg ttgtatttta
     5581 atattgttaa tgattgtata ttaattgctc cttatagcct cgttcctaaa tcgtattaaa
     5641 aaaagaattc ttgcaaaatt actccatatt ttcagtatca accttgatga aaccatgttc
     5701 tacaacatag tttttgcatt attatctttc gttcagatcg ggttctgccg gtaatagcga
     5761 gcccttgatt ttggtctcgt ccgagataaa tacgggggtt tgaatttctt tacgggccga
     5821 tcgctgagta gcaacgcttc tgtccaaaat caaagaaatg gccgcattca acagcaattc
     5881 cgaggtatct cccaaagcag attgatcgta aacgctcaac tctgtcaatt ctatatccgg
     5941 ttcaaaaccg gttgtataat ccgattctcc caggctgttg aacaccttgg caacgatggg
     6001 ctgcatgccc cagcgatttt tgggattgtt ttgttcgtat atgctcaacg aagacacatt
     6061 cttaccatat gtagtcttac caataagaat aacaggcata taagctttta gtccgttgat
     6121 cagttgctcg ctggccgagg cggtatattc cccggtcagt acgatcaggc gttccagatt
     6181 taaattgttg agcaaaacag ccaagctgga gcctgcaggg aaaaaattct ccttaaaatg
     6241 catcaggaaa taatcttctc cgtattgttt gttgtaaaat gcctgcaaat agtcgttgta
     6301 tcggaaatag caaaaaactt tatcccggct cagcgcaggc aatatcatgc tggacaaata
     6361 agtagacatc ataacgtaac cgccgctgtt gtaccttaag tccagcacca gctcgtcgac
     6421 atcaagggct ttgaaacgac cgaaaatctc gtttgtttcc aacaaaaaac ggtcgctctt
     6481 atcttcaggg ccaaaggcaa aaaaattata caaaaagtaa gctatctttt tgttccctat
     6541 ttgatagacc gtgtccaggt aattgggatt ctctgcgtat ttttcgagca cggcaaaagt
     6601 cagagtgtct acgtgcatcg cattgaggta agcctcaaag ttttccgggg tttgttgtgg
     6661 tgcccggttc aggaaacgaa gagtgatttc cgcaggcaaa ttcttgagca aatctgtgta
     6721 attgtcggta ttcagcgcaa ttccccctat ctggtcaaac caccagcctc tctgtacacc
     6781 tgcatccaca gcaggcgtat tgggtttaac ataagtcacc tgccccacta tagccttgtc
     6841 gttttccgac cgcaaataca attgataatc aaagcctatt tcccgtgaac gaagtccgct
     6901 aaaggtattc aacaaattgg cgtaatcgct tcgaataaaa gaaaagcggt cgccgtcggg
     6961 agctgtcgta ggatggtaac gataaagcaa gtcattaaaa aaggagatcg gtgactgagt
     7021 gtaatcttcg ggctctatca tcttatcgct ccagtaatac gccagggaca taccgttgta
     7081 gatccattta ttcacatagc tattgttcag actgaggctg tccgcttctt cttttccatt
     7141 atgtgtaaac gagctttctc cctcacaaga agcaaacaag caagccagaa aaaccaggga
     7201 ccagcttata agcgatttcc ttatcatctc aataaatatt ttttcataaa gctttataca
     7261 taatttggtc tattctttga aatacaccct tttgactctt tcagatacag aagtcagcac
     7321 ttcgtacgaa atggtagata agcgttctgc cagttccgtc aaaggcagtc ccttaccaaa
     7381 caacaccacc gtatcgcctt cgtgggcagc tatatctgtt acatccacca tacacagatc
     7441 catacaaata ttgcccaata tttttgctct ctgtccgttg accaatacgt ctccaccggt
     7501 atgatgccgg ttgaaaccgt cgccgtaacc taccggtaaa gtggctatac gtgaatccct
     7561 gtcgagcaag acagttcggc tgtaccctat actttcgcct tttttaaatt catgaatctg
     7621 cagtattctg gttttgagag aagcaaccgg ctgtaaggcc tcgggactac aaaccgacac
     7681 tccgtaaagc cctataccca gacgcaccct gtcgcattgc cattgaggga aacgttctat
     7741 accggcggaa ttcaaaatat gacgttcaaa acgatagggt aaggcccggc tcatttgccg
     7801 ggtgtagagt ttaaacagtt tccattgagt ctttgtatac ttatcaaaag cattttcgtc
     7861 actgcccgcc agatgagaaa aaacagaact gactctcaat tcaggccggc tgttgagttt
     7921 tgacaacagt tccggcaagt ctttttcggt gaaacccagg cgatgcatac cggtatcgag
     7981 tttgatgtgc acgggaaaat ctcggactgc cagacgttcg gctgaagcca taaaagcatc
     8041 cagtaaagcg aaactgtaaa tctccggctc cagtttgtat tcgtaacaga gaggaaaaat
     8101 tccgggatcg ggattcatta ccaaaatagg aatatggata ccgccacgtc tcaatttcac
     8161 cccctcatca gccagagcca cggccagagc ttcacaaccc tgggcctgta aagtcttgct
     8221 tacctcaaga gctccgtgcc cgtaggcttc cgccttgacc atggctatta ccttggtttg
     8281 aggcttgatc agagttctga agtaattcag gttatgcacc agagcgttca ggtcaacctc
     8341 catgacggtg ttgtgtgttt tcaattccag gcgttcgctg atgcgttcga aaccgaattg
     8401 tctggaacct tttatcaaaa tcaattcatc ttgaaattga tcgggcaaat ccgagagtaa
     8461 aaaatcgtcc gtggtcaaaa aaaagcgagc agaagagggg aaaaatccgg catgagttac
     8521 caggccgtaa cctataccaa tgaagcgatc tatctgtttt cgcttaatca gttcagccac
     8581 cgtaccgtat aaatcgtcca tagacttacc gctttcgaag atgtcggaca agattaaaca
     8641 agatcgcatt tttccggcta aagcccgcct cgattgaaaa tccaaagcca gctcaagagc
     8701 ctgtaaatct gagttataag catcgtctat caaaacacag ccattcctgc cctctttcac
     8761 ttccatacgc atggccaccg gttcgagttg cttcaaacgc ccgccgagta ccgaagcatc
     8821 tattacaaag tctgtttcct gttcaagcag aaacaaaaaa gcaatgcaat gcagggcatt
     8881 ttttatagaa ggttcatcta caaaaggtaa aacaaaacag acttcttccc tgtgccacaa
     8941 agtatgaatc tgggtatagt ctttttgttt gtctttgcga aaatacagtt ctgcgctcag
     9001 gtcgttgcat gaccatgcca aaagacgttt aggttccagc attccggcca gaataccggc
     9061 tgacggttct tcgtcctgac agtaaattac ccgtttacag tctttgaaca agagcaattt
     9121 ttcccgtacc aaactctcga cggaatcaaa gttttcgtta tgagcctggc caatatgcgt
     9181 caataaaccc caatcgggct gaatcatgtt ttgcaacttt tgcatttcgc ccacttgaga
     9241 aatcccggcc tcgataaggc ccaaacgact gtcttcgcta agcatggaca gggacaaagg
     9301 cacacccaaa cgggaattgt agctgcccgg cgaacggctg atgctgagat tatcgcgcaa
     9361 cagttgccaa atccactctt tcactatggt tttaccattg ctgccggtta tgcccaacac
     9421 tttcccttca aaaattttcc tgcagtatcg gcccaataat tgcagagctt cgatggcatc
     9481 ttccacatag agaaaattag cctgatcaaa gagcgattcc ggctgttttt cgctgagtac
     9541 aaaatttctt accccccttt tgtataaatc ggacaggtat ttatgcccgt tgttgctttc
     9601 ggttttaatg gcaaaaaaca aacagccctc cggcaccaac aaagccctgc tgtctatgag
     9661 cagacgttca atgacggtgt cctgcacaat tattgctttt tgaaccccga ttaggcgggc
     9721 tatttcacta acaggtagat gcattggaat gtaatactga ttcaaaaaaa actctttcct
     9781 tttccagatt cagttcggga aagcgcttgc ccaacaaatg cagggctgct atatttttct
     9841 taataaaacg aagatactct tcgtcgtcgg gacgaaatgg tttatggtcc attgctttgt
     9901 gaatttgttc caactggcga atgatttcga gggtatattt ttcgaaagca aattcaccaa
     9961 aacgctccca gatatactga aaagccatag ccgacgggtg tagcatatcc tgttcgtaga
    10021 agcgataatc ccttagatcg tccatcagca attcgtatgc gggaaaatat accatctgtt
    10081 cgaaacgaga gcacaactcg tcgaccaaca gatgcaaact agccttactc agagtgtttt
    10141 cgtgtgcgcc atccttaaag tgacgtatgg ggcttactgt caaaagcacc tttaatttgg
    10201 gcctggtccg aaacaactcc tcaaaaaaac cgactgtttg ttccagcatc tccccgacag
    10261 acaaacgtct gcgatcgaaa aaggaagccg gcaatttgtg gcaattcgaa acgacaagcc
    10321 cattggacgc taattcgtat acccaggctg ttccaaaact aaggataagg caagttgaag
    10381 atctaaaggc cgacgacgat gttgaacagg ctgtttgaat accctccagt actttttcgg
    10441 cctcccttcc cgaaaaggag gaatgaaagt caaaactgcc gtaaaggcct ttgtattcaa
    10501 aaatatcctc tttgctgtag gatttattct cttgcatcgc cctgagagcc cttattacag
    10561 acgaaggatt ataaagcact ccgaaaggat tgtttaggca gtcgaaacca cttttttgta
    10621 attgctttcc tacggattct gcgaaacagg aacccagaca gagaatgctg tctgaatggt
    10681 ttagtcgaaa actgcttttt ggcagtgtta aagtggtctt gaagtccata attggataca
    10741 aaaatacaca tattttccct tgattataga atttatttac ctaattcatt tattatggaa
    10801 aaagtcttac ttttgctgtc gctatgatgg aaaggaaaac ttctttgttg gacgaaatac
    10861 aataccccga ggatctcaaa aagtgctcag tctcgcaatt aacccaagtt tgcgaagaac
    10921 taagacaatt tatcattgat gcactctccg aaaatcccgg acacctgggt gccagcctgg
    10981 gtaccgtaga actgaccgtg gctttgcatt atgtgctgaa tacccccaac gaccggattg
    11041 tttgggatgt ggggcaccag gcttacgggc acaagatcct gaccggccgt aaaaaactct
    11101 tttataccaa ccgcaaactt aacgggatca gcggttttcc caatccttta gagaatgagc
    11161 acgaccattt tattgccgga cacgcttcca attccatctc ggcagctttg ggtatggata
    11221 tggcagccgc tttcaaaaaa gaaaaaagag tggttgtagc cgttatcggt gatggtgcca
    11281 tgaccggagg tttagccttc gaaggcctca acaacgcctc tgtttaccca aacaacctgc
    11341 ttatcatctt aaacgacaac cagtatgcca tagacaaaaa catcggcggc gtcagccgtt
    11401 acctttc
//
LOCUS       DAOSTD010000061        11159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_5743_length_11159_cov_14.288094, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000061 DAOSTD010000000
VERSION     DAOSTD010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11159
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5743_length_11159_cov_14.288094"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..1404)
                     /locus_tag="PLI37_05290"
     CDS             complement(247..1404)
                     /locus_tag="PLI37_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOQ58455.1"
                     /translation="MGDFLQFLGENIKEFLTYTGFANATSGHLIMILVGLVFIYLAVR
                     HEFEPMLLIPIGFGMLIGNIPFKEVGLELGIYEEGSVLNILHQGVLKGWYPPLVFLGI
                     GAMTDFSSLISNPKLMLVGAAAQIGIFASYSLALSLGFDPKQAAAIGIIGGADGPTAI
                     FLSSKLAPNFLGAIAVSAYSYMALVPVIQKPVMRLLTTKKERVIRMKPPRIVSSTEKI
                     VFPIAGLLMTAFMVPSGLPLLGMLFLGNLLKESGVTRRLAETARGPLIDTITILLGLT
                     VGASTQASQFLTWDSIKIFTLGAFSFIIATAGGVLFVKLFNLFLSKDSKINPLIGNAG
                     VSAVPASARISQVMGLKYDPKNYLLMHAMGPNVAGVIGSAVAAGVLLGFLG"
     gene            complement(1406..>1798)
                     /locus_tag="PLI37_05295"
     CDS             complement(1406..>1798)
                     /locus_tag="PLI37_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOQ58456.1"
                     /translation="YKVEIINEQKNLIELEVNGTPYTVQIERPEKSKLITTKPAAAPV
                     TPEGTPVVAQAKTKSPGGAVKSPLPGVILSVNVKVGDQVKIGSKLLVLEAMKMENTIS
                     SDRDGKVLEIKVEKGASVLEGAELVIIG"
     assembly_gap    1799..1898
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1899..2581)
                     /locus_tag="PLI37_05300"
     CDS             complement(<1899..2581)
                     /locus_tag="PLI37_05300"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOQ58457.1"
                     /translation="MINNKTNFIDEYGQRSAWFELFNASYGIVDIGGCYLSNDRNNPT
                     MYMIPKGDVLTKIQQRQHVLFYADGTPHKGNFYVNFTLDTASINVLYFFNADGKSLID
                     SVVVPILPADLSYARTIDGEENWEIMRRVTPSTNNLTLDRNEKVEKFAKNDPYGVGMT
                     LSAISVVFSVLVLLSLFFYWFAIVFRKVTAKPKKTGILRSPVRADEIPGEVIAAISAA
                     LYELDNDVH"
     gene            complement(2674..4227)
                     /locus_tag="PLI37_05305"
     CDS             complement(2674..4227)
                     /locus_tag="PLI37_05305"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOQ58458.1"
                     /translation="MSNQLEKVKELIAFREQARLGGGIKAIEKQHAKGKYTARERINM
                     LLDEGSFEEFDMFVKHRCTNFDQDKKHFLGDGVVTGYGTIEGRLVYVFAQDFTVFGGS
                     LSETMSLKICKVMDQAMKMGAPVIGINDSGGARIQEGINALAGYGEIFERNILASGVI
                     PQISGIFGPCAGGAVYSPALTDFTLMTKGTSYMFLTGPKVVKTVTGEDVTQEELGGAS
                     VHATKSGVTHFAVDNEEEGIALIRKLLGYIPQNNLEEAPMKDCTDPIDRLEDSLNDII
                     PDSPNKPYDMYEVIGAVVDNGEFLEIHKDYAKNIIIGFARFNGQSVGIVANQPKMLAG
                     VLDCNASRKGARFVRFCDAFNIPIVSLVDVPGFLPGTGQEYGGVIVHGAKLLYAYGEA
                     TVPKVTVTLRKSYGGAHIVMSCKQLRGDINYAWPTAEIAVMGALGAVEVLYAKEMAAA
                     EDPAKLSAEKEAEYSEAFQNPYKAAQYGYIDDVIEPRNTRFRVIRALQQLQTKKLTNP
                     AKKHGNIPL"
     gene            complement(4240..4650)
                     /gene="mce"
                     /locus_tag="PLI37_05310"
     CDS             complement(4240..4650)
                     /gene="mce"
                     /locus_tag="PLI37_05310"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOQ58459.1"
                     /translation="MKPTHIEHLGIAVKSLETAIPYVENIFGLKCYAIEEVPEQKVKT
                     AFFKVGQTKIELLESTDPEGPIGKFIENRGEGVHHIAFAVENLQEKLDTVAEKGVRLI
                     DKTPRKGAEGLNIAFLHPKFTLGVLTELCENPNK"
     gene            complement(4743..5777)
                     /locus_tag="PLI37_05315"
     CDS             complement(4743..5777)
                     /locus_tag="PLI37_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOQ58460.1"
                     /translation="MNNNSGIIDALLSGIADAQASHSNIQTIYTDAEATFLKNNALFF
                     IKPEITRADPAIRLKEVLTLILDKLAAFELRIHSIRILPAAYLKKHDLIRSHYGVISR
                     VASEGAKTLGDEAKGRFREVFGLEPSEARILGGLEVLETYPFFNSRSLDCLWQNGQNH
                     KLAGGTYAEKWRIDSDTVYVLNAFHPRQLEHFTQPGRSIVVMNISGDTDWSDLRNSFA
                     GATNPQKAMPGSLRNDLCLRARELGLGEISQSQNGVHLSAGPVEALLELQRFESDLDR
                     DKALLPFETFPFGRQLLTVFEQIPVKILNNEKINHQGKQVSIFDLTEEMNAAEALSFL
                     KGLAETGIFM"
     gene            complement(5789..6259)
                     /locus_tag="PLI37_05320"
     CDS             complement(5789..6259)
                     /locus_tag="PLI37_05320"
                     /inference="COORDINATES: protein motif:HMM:NF016885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HOQ58461.1"
                     /translation="MKYLTELNSLLPLLGHRNWILVVDKAYPLQSSKGISYLNSGEDL
                     NKVLKEVLNALEAASHLRPIIYLDKELEFMDDELCKGVSDFKTTLQRICSNYTLKQIP
                     HEDIFRRLDEAARLFNVVVIKTESQIPYTSVFIELDCGYWSEEKEKTLRNKIVL"
     gene            6465..7346
                     /locus_tag="PLI37_05325"
     CDS             6465..7346
                     /locus_tag="PLI37_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58462.1"
                     /translation="MQQKTLYILLACIVLLFASGVFLFIDRHSKSRLINEITAQFELE
                     KEELEDGYSQLALQYESYGIRIANDSLAELLDIERTKNLRLLEEIRTLKINNVVRINE
                     LRKELETARSVMRSYIIQIDSLNTLTTRLRKENETVSRQYREIKQEASQLAKANTELT
                     EKVVLASILEARDINIRTLNERERVINRLSKIATIEFNFYLPKNISAKVGEKNIYLRI
                     LQPDNTPLVKNEGNLFEFEGTKLNYSIFRPIEYAGEETPVSLYWKVEEFLYPGTYRAD
                     FFADGFLIGSKSFELLN"
     gene            7348..9915
                     /locus_tag="PLI37_05330"
     CDS             7348..9915
                     /locus_tag="PLI37_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58463.1"
                     /translation="MRKHSSAMGRILFSGALFCFSVLFSVQSSEKGFKDFFSSYNHFS
                     QAYTQEKVYLHFDNNSYFIGETIWFKAYVLLAENHHYSTMSKTLYVELLNSRGDVLIT
                     QKLKIENGQCSSGIFLPNEWYADYYEVRAYTRCMLNFGEGVVFSRVFPVMDAIGYDKK
                     TGEPKPPAMTFSKSDLPIKRVDLKPQGNLSLDFYPEGGHLIEGLPSVVAFKAIGKQGE
                     DALISGNLYNSRKEVVTFFSSMHQGMGYLEFTPEPGQQYKAKVLYDDREYEFSLPAAL
                     PAGYALRVSNQNPAYLTVHIATKSGEKNDSVGLAVTCRGKIYARAVILPQPEQILQIP
                     IQALPSGVQQITLFNRKGEILAERLAFVRNPFYDPLRYNISIKQLNENPDIFRPVRLE
                     VEVETARNSRLTDSNTVKTLPAGTSLVLSVRDAAENLKNAPLSNIYSDLLLSSELKGY
                     IHNPAWYFEKEDLRRSKALDLLMLVQGWRRYDWEIMAGSKPFEAKHPIEEGIVLQGEI
                     ISQLLKKPRKDIRVTIWMTSDSSAYYGNCLTDSAGTFNFLFDFVDTWKLSLQITDGEK
                     RKNHRIVLDRLFSPAPKSFAYYEREAPVYERLRFPKKTVAGRLPSVIDSSKITVLPGE
                     VLLPQAEIKRRRSEQLTTGEASVIYAVEREVDVIRDRAENEYSSIPIFLTQINPYFWL
                     GQKYSSSSDSMEDVMQYKGKDVVFVLEDLYTIGMNSEEMPYVDEVENIAIVESNTPNL
                     FYESPEAVATMKSRVYVHLYRYKDRRRNIDPLGIRKTTLQGYSRVKEFFHPKYDRVLM
                     SDPQDHRRTLYWNPSLTTDSTGKAQVLFYNNASCRKMSFDAVLLTEDGKMGLLKAEP"
     gene            complement(9999..11114)
                     /locus_tag="PLI37_05335"
                     /pseudo
     CDS             complement(9999..11114)
                     /locus_tag="PLI37_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
BASE COUNT         3123 a         2581 c         2364 g         2991 t
ORIGIN      
        1 aaatacagtt gggaggatta ctatctttcc ggtttgaccc ccgtcgtatt tttaaagaaa
       61 atcttacaaa tataaatcta aatcgtttta aacaaataat aattatgaac aatttatatt
      121 aacaggcaag ctgatcgatg taacgagtaa gatatcgggt aatgcacaaa aaaaagagag
      181 tgtccttttt gtaagaacac tctctcgaga atgttggtta tggtattttc cccaaaggaa
      241 taaacattag cccaaaaatc cgagcaaaac acccgcagcc acggccgacc cgataacacc
      301 tgccacattg ggacccatgg cgtgcatcaa taaataattt ttgggatcgt attttaatcc
      361 catcacttgg gaaattcttg cagaagcggg gacggcagac actcctgcat ttcctattaa
      421 aggattgatt ttgctgtctt tggaaagaaa caaattgaat aatttcacaa aaagaacacc
      481 gccggctgta gcaataataa aggagaaagc tccaagagtg aatattttta ttgaatccca
      541 agtaaggaat tgcgaagcct gagtagaagc tcctaccgtc aaacccaata atatggtgat
      601 agtatcaatc agcggacctc ttgcggtctc ggccaaacgt cttgttacac cgctttcttt
      661 gagtaaattt cctaaaaaga gcatacccag caagggcaaa cccgaaggaa ccataaatgc
      721 agtcatcaac aaaccggcaa tcgggaaaac aattttttcg gtagaagaaa ctatacgtgg
      781 aggtttcatc cggatgaccc tttccttctt tgtggtcagc aatcgcataa ccggtttttg
      841 aatcacagga accaaggcca tatatgaata ggccgaaacg gctatagctc ccaaaaagtt
      901 gggtgccagt tttgacgaaa gaaatattgc cgtaggacca tcagcacccc caataatacc
      961 gatggctgca gcttgtttgg ggtcaaatcc caaactgagt gccaaagagt aagaagcaaa
     1021 tataccgatt tgagcagctg cccctacgag catcagtttg ggattggaaa taagcgacga
     1081 aaagtctgtc atggctccaa taccaaggaa gaccaatggc ggataccagc ccttgagaac
     1141 gccttgatgc aaaatattaa gcacggagcc ttcctcgtaa atacccaact cgagacccac
     1201 ttctttgaag ggaatattac cgattaacat cccaaaacct ataggtatca acaacatggg
     1261 ctcaaattcg tgacgtacgg caagataaat gaataccaag cccaccagta tcataatcaa
     1321 atgtcccgaa gtcgcattgg cgaaaccggt atatgtgaga aattctttaa tattctcgcc
     1381 taaaaattgc aaaaaatctc ccatatcaac ctataattac cagttcagca ccttcgagca
     1441 cagaggcccc tttttctact tttatttcca acacttttcc gtctcggtcg ctagaaatag
     1501 tattttccat cttcattgct tccagcacca ataattttga gccaatttta acctgatcgc
     1561 ccactttgac attaacactc aaaattactc cgggtaaagg agattttacg gcccctccgg
     1621 gactttttgt ttttgcctga gccactaccg gtgtcccctc gggagtaaca ggagccgcag
     1681 caggtttagt agtgatcagt ttgctttttt ccggacgctc tatttgaact gtataaggtg
     1741 tcccgttaac ttcaagctca atcaaatttt tttgttcatt gataatttca actttatann
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc gtgaacatcg ttatctaatt
     1921 catacagagc agcagaaatc gcagcaataa cctcacccgg aatttcgtca gctctcacag
     1981 gactccgaag tatacctgtt tttttaggtt tcgctgtgac cttacggaaa actatggcaa
     2041 accagtaaaa aaagagggat agcaacacca atacggaaaa aacaacagaa atcgccgaaa
     2101 gagtcatccc tacaccatag gggtcattct ttgcaaactt ttctactttt tcattcctat
     2161 ccaaagtaag gttgttggtg ctgggggtga cgcgacgcat aatctcccag ttttcttcgc
     2221 cgtcgatagt acgggcatag ctgaggtcgg caggcaatat cggaacgacc acactgtcga
     2281 ttaagctttt accgtcagcg ttaaaaaagt acaaaacgtt tatactggca gtgtcgaggg
     2341 tgaaattgac ataaaaatta cctttatgcg gtgtaccgtc ggcataaaac aagacgtgct
     2401 gacgttgttg aattttggtc aggacgtccc ccttaggtat catatacata gtgggattgt
     2461 tccggtcgtt acttaaataa cagccgccaa tgtcgactat accgtacgag gcgttgaaca
     2521 attcaaacca cgccgacctt tgaccgtatt catcaatgaa atttgttttg ttgtttatca
     2581 acacttcatt gaaacagacc cgggtcacct gggcgtaaag cgctgtactt aaaaacgtaa
     2641 ttaaaacaaa aaaaccgatt cgtatcttca taattacaag ggaatgttac cgtgtttttt
     2701 ggcaggattg gtcaattttt tggtttgcag ctgctgcaaa gctctgatga cgcggaaacg
     2761 tgtgtttctg ggttcgatta catcatcaat gtaaccatat tgagcggctt tgtagggatt
     2821 ctgaaaagct tcgctgtatt cagcctcttt ttcggctgac aatttagcgg gatcttcggc
     2881 agctgccatt tctttggcgt acaaaacttc aacagcgccc aaagcaccca taacggcaat
     2941 ttcggctgtt ggccaggcat aattaatatc gccgcgcaat tgtttgcaac tcatcacaat
     3001 atgagctcct ccgtaggatt tgcgcaaggt tacagtcact ttgggtaccg tggcttctcc
     3061 ataagcatac aacagttttg ctccgtgcac aattacgcca ccgtattcct ggccggtacc
     3121 gggcaaaaat ccgggcacgt caaccaaact cacgatagga atgttgaagg catcgcaaaa
     3181 acgaacaaag cgggcccctt ttctggaggc attgcagtcg agtacgccgg ccagcatttt
     3241 gggctgattg gccactatac ccaccgattg tccgttgaaa cgggcaaaac cgatgatgat
     3301 attcttggca taatctttat gtatttctaa aaactcccca ttgtctacca cggcacctat
     3361 cacctcgtac atatcgtagg gtttgttggg gctgtcgggg ataatatcgt tcaaggaatc
     3421 ttccaggcga tcgatgggat cggtgcaatc cttcattggg gcttcttcaa gattattctg
     3481 aggaatataa ccaagtagtt tccttatcag agcgatgccc tcttcttcgt tatctacggc
     3541 aaaatgggtt actcccgact tggttgcgtg taccgaggcg cctccgagtt cttcctgagt
     3601 gacgtcttcg cccgttactg ttttcaccac tttgggaccg gtcaaaaaca tataggacgt
     3661 tcccttggtc atcaaagtaa aatcggtcaa tgcaggagaa tatacggctc cgcctgcaca
     3721 gggaccaaaa atgccggaaa tttgaggaat gacacccgaa gccagaatat tgcgttcaaa
     3781 aatttcgccg taaccggcca gggcattgat accctcctga atacgggcac cgcccgagtc
     3841 gttgatcccg ataaccggag cgcccatctt catggcctga tccattactt tacaaatttt
     3901 cagcgacatg gtttccgata aggaacctcc aaagacagta aagtcctggg cgaaaacata
     3961 caccagacga ccttcaatgg ttccgtaacc ggttaccact ccatcgccca aaaaatgttt
     4021 cttgtcctga tcgaaattgg tacaacgatg ttttacaaac atatcgaact cttcgaaact
     4081 gccttcgtcc aaaagcatgt tgatacgctc acgagcagta tatttccctt tggcatgctg
     4141 tttttcgatc gcttttattc cgccgcccaa acgagcttgt tcgcggaaag caattaattc
     4201 ttttactttt tccagttgat tactcatcga tattagtatt tatttgttag gattttcgca
     4261 caattcagtc aatacaccca atgtgaattt cggatgtaaa aaggcgatgt tcaggccttc
     4321 agctccttta cggggagttt tgtcgatcag acgtacgcct ttttcggcta ccgtatcgag
     4381 cttttcctgt aaattttcga cggcgaaagc aatatgatgc acaccctctc cgcgattctc
     4441 tatgaattta cctatgggcc cttcgggatc ggtcgactcc agcaactcga ttttagtttg
     4501 tccgaccttg aaaaaggctg tttttacttt ttgttcgggc acttcttcta tggcatagca
     4561 tttcaggcca aaaatgtttt caacgtatgg aatggcagtc tctaaactct tgactgcgat
     4621 gccaagatgc tcaatatgtg ttggtttcat aattatgaat taagttttaa aaaaaacgct
     4681 gcaaagatag gaattaatat ccggcttagg tccggggaaa aacccaaaaa atgtattatt
     4741 ttttacataa atattcctgt ttcagcaagc cctttcaaga aactcaaagc ttctgccgca
     4801 ttcatttctt cggtcaggtc gaaaatggag acttgcttgc cctgatggtt gattttttcg
     4861 ttgttcaata ttttaaccgg aatttgttca aataccgtaa gtagttgacg accgaagggg
     4921 aaagtttcaa aaggcaataa ggccttgtcc cggtcaagat ccgattcaaa acgctgtaat
     4981 tccagcaagg cctctacggg tcccgccgac aagtgtacgc cgttttgact ttgggagatt
     5041 tcgcccagac ccaattcccg ggcccgaaga cacaggtcgt ttctgagact cccgggcatg
     5101 gccttttggg gattggttgc tccggcaaaa ctgtttctca aatccgacca gtctgtatcg
     5161 cccgaaatat tcattaccac gatgcttctg ccgggttggg taaaatgctc caattgcctt
     5221 ggatggaaag cattcaacac gtataccgta tctgaatcta tacgccattt ttcggcatag
     5281 gtgccgccgg ccagtttatg gttttgaccg ttttgccaca ggcaatccag cgagcgtgaa
     5341 ttaaaaaagg gataggtttc caacacttca aggcctccca gaatacgagc ctcactgggt
     5401 tccagtccaa aaacttccct aaaccggcct ttagcctcat cgcccagtgt tttcgccccc
     5461 tccgaggcca ctcggctgat tactccgtag tgactacgta tgagatcgtg cttcttcaaa
     5521 taagcagccg gcaaaatacg aatgctgtgt atgcgcagtt cgaaagcagc cagcttatcc
     5581 aaaatcagcg tcagtacctc tttgagccgg atagccggat cggctctggt aatttcgggc
     5641 ttgataaaaa acaaggcatt gttttttaaa aaggtcgcct ctgcatcggt gtaaatggtt
     5701 tgaatgttgg aatgcgaggc ctgagcatcg gctataccgc taagcaatgc atcaatgatt
     5761 ccggagttgt tgttcatgct gtttaagatt aaagtacgat tttgtttcgc agggtttttt
     5821 ctttttcttc cgaccaatat ccacagtcca attcaataaa tacggaagta taggggatct
     5881 gtgattccgt ctttattacc accacattaa acaatcgggc agcttcgtcg agtcgacgga
     5941 aaatatcctc gtgcggaatc tgtttcaaag tgtagttgga acaaatacgc tgcaaggtgg
     6001 ttttaaagtc acttaccccc ttacacaatt catcgtccat aaattccaat tctttgtcga
     6061 gataaattat ggggcgcaga tgtgatgctg cttccagtgc gttgagcact tccttgagca
     6121 ctttattcag gtcttctccc gaattaagat aactgatgcc cttagacgat tgcaaaggat
     6181 aagccttgtc caccaccaaa atccagtttc gatggcccag caagggcaat aaactgttca
     6241 actctgtcaa atatttcatg ttgtataatt atgataaggt tttcagaatt tcctctatat
     6301 aaaaaatctt tccgaggacg aaagtagtaa ttatatctca attagaagtc atcttttaga
     6361 gccttaactc ttaaagcatt atttattatt tttttttatt tgtacgaaat ataacggggc
     6421 aattttacta tctttgcccc gtcaccctgc aaagattgat gttcatgcaa caaaagactc
     6481 tatatatttt acttgcctgc attgttctgc tttttgcctc aggtgttttt ttgtttattg
     6541 acagacacag caaaagccgg ttgatcaacg aaattaccgc gcagtttgaa ctggaaaaag
     6601 aagaactcga agacggatat tcccaactgg ccttgcaata cgaaagttac ggcattcgta
     6661 ttgccaacga ttcgctggcc gaactgctcg atattgaacg aaccaaaaat ctgcgtttgc
     6721 tcgaagaaat caggactctt aaaatcaaca acgtggtccg tatcaacgag ctccgaaaag
     6781 aattggaaac ggctcgcagt gttatgcgat cctacattat tcagatcgac tcgctcaaca
     6841 ccctgactac gcgtttgcgt aaggagaacg aaaccgtatc cagacaatac cgtgaaatca
     6901 aacaggaagc ctcccaattg gccaaagcaa ataccgaact gaccgaaaag gtggttcttg
     6961 catccatact tgaagcccgg gatataaata tcaggacctt aaacgaaagg gagcgcgtaa
     7021 taaaccgttt gagcaaaata gctaccatag aattcaattt ttacctgcct aaaaacattt
     7081 cggccaaagt gggcgaaaaa aatatttacc tgcgcatact ccaaccagac aacactcctt
     7141 tggtaaaaaa tgaggggaat ttatttgagt tcgagggtac taagctcaat tattccattt
     7201 ttcgtccgat agaatacgcc ggagaagaaa ctccggtcag cctgtactgg aaagtcgagg
     7261 aatttctgta tccgggcact taccgggccg atttctttgc cgatggattc cttatcggca
     7321 gcaagtcctt cgagttgctc aactaatatg agaaaacatt cctcagcgat ggggcgcatc
     7381 ctgttttcag gggcattatt ctgcttttcg gtattattct cagttcaaag cagcgaaaaa
     7441 ggctttaagg attttttcag ttcttataac catttcagcc aggcttacac tcaggaaaaa
     7501 gtctatttac atttcgacaa caactcttat tttataggtg aaaccatttg gttcaaagcc
     7561 tacgtgctcc tggccgaaaa ccaccactat tcgaccatga gcaagaccct ttacgtggaa
     7621 ttgctcaaca gcagaggaga tgtgcttatc acacaaaaat taaagataga aaacggacaa
     7681 tgcagcagcg gtattttttt gcccaacgag tggtatgcag actattacga agtaagagcc
     7741 tacactcgct gcatgcttaa ctttggagaa ggtgtggttt tttcccgggt ctttccggta
     7801 atggacgcca tcggttacga caaaaaaacc ggggaaccca agcctccggc tatgactttc
     7861 agcaaaagcg acctgccgat aaaaagagtg gatcttaaac cgcaagggaa tttgagcctg
     7921 gatttttatc ccgaaggagg tcatttaatt gaaggactgc cctcggttgt tgcattcaaa
     7981 gcaataggca aacaaggtga agacgctttg atatcgggca atctgtataa cagccgcaaa
     8041 gaagtggtta cttttttcag cagtatgcat cagggaatgg ggtatctcga attcaccccc
     8101 gaacccgggc agcaatataa agcaaaagtt ttgtacgacg accgggagta tgaatttagt
     8161 ttgccggcag ccctgcccgc gggctatgct ctgagggtaa gcaatcaaaa tccggcttat
     8221 ttaaccgtcc atatcgcaac aaagtccggt gaaaaaaacg actccgttgg cctggcggtg
     8281 acctgccggg gtaaaatcta tgcccgggcg gtcatcttgc cgcaaccgga acaaattctc
     8341 cagataccca tacaagcatt gccctcgggt gtgcaacaaa tcacactttt caaccgaaaa
     8401 ggagaaattc tggccgaacg cttggctttt gtcaggaatc cgttttacga ccccttacgg
     8461 tacaacattt ccataaaaca actgaacgag aatccggata tttttcgtcc ggtcaggctc
     8521 gaagtggaag tggaaaccgc ccggaactcc cggctgacgg acagcaatac ggtcaaaacg
     8581 ctgccggccg gcacctccct ggtgttgtcg gtacgcgacg ccgccgaaaa cctgaagaat
     8641 gcgcccttga gcaatatata cagcgattta ttgctttctt ccgaactcaa gggttatatc
     8701 cacaatcctg cctggtattt tgaaaaagaa gatctgagac gaagcaaggc tctcgattta
     8761 ctgatgctgg ttcagggctg gagacgctac gactgggaaa taatggcggg aagtaaaccg
     8821 ttcgaagcca aacatcctat agaagaaggt atcgtattac agggtgagat tatttcccag
     8881 ctattgaaaa aacccagaaa agacattcgg gtaaccatct ggatgacttc cgacagctca
     8941 gcctattacg gaaattgcct gaccgattcg gcgggaacct ttaatttcct gtttgatttt
     9001 gtggatacct ggaagctttc gctacaaatc acggatgggg aaaaacgcaa aaaccaccgt
     9061 atagtgctgg acaggctatt cagccctgca ccaaaatctt ttgcttatta cgagcgtgaa
     9121 gctcccgttt atgaacgatt gcgatttcct aaaaagacag tcgccggcag actgccaagt
     9181 gtcatagaca gttccaaaat cactgtactg ccgggagaag tcttattgcc gcaggcagaa
     9241 ataaaaagga gacgatccga gcagcttact accggagagg ccagcgtaat atacgccgtc
     9301 gaaagggaag tagatgtaat ccgggatcga gccgaaaatg aatactcgag cattcccatc
     9361 tttctgaccc agatcaatcc ctatttttgg ttaggccaaa aatacagctc ttccagcgac
     9421 tccatggagg atgttatgca atacaaaggc aaggatgtcg tttttgtcct cgaagattta
     9481 tacaccatag gtatgaattc cgaggaaatg ccttatgtgg acgaagtcga aaatattgcc
     9541 atagtggaat ccaacactcc caaccttttc tacgaaagcc ccgaagcagt ggccactatg
     9601 aaaagccggg tttatgtgca tctctaccgc tacaaagacc gacgtcgcaa tatcgacccc
     9661 ttgggcatcc gtaaaaccac tttgcagggc tacagccggg taaaggaatt tttccacccc
     9721 aaatacgatc gcgtcctgat gagtgatcca caggaccaca ggcgcacttt gtactggaat
     9781 ccgtcgctca caaccgacag cacaggcaaa gcccaggtgc ttttttataa caatgcgagc
     9841 tgcagaaaaa tgagtttcga cgccgtcttg ctgaccgaag acgggaaaat gggattactc
     9901 aaggctgaac cctgattttt ttgacttctt tgggctcgga ggacttattc gagattgggt
     9961 gctattcggg ctcgggtggc tggggttcgg gggacttatt ccggctcggg aaaatgaatg
    10021 tccgaacgtc ccacaggtct ggacgttgcc acggcaatac cccgctgctc gtctttattt
    10081 accgcacaat aaaaatgata taccgtccct tggtattcga ttaaaaaaga tttgtgcgcg
    10141 aataagtcat cgtaatcttc ggagggtatt atcagatcgg gtccctgcca ttctgtccag
    10201 ttgatcaaat cgtaggaaca ggcaaaagta ttgaaagcct tataaggcct gtctttccaa
    10261 aaagcaccaa aataaaacat cacatagaga tcgcccattt tctggatgtg ggcatctcct
    10321 gtaatcatgc catcctcatt accgaaaacc ggattgcccg gataacgctc ccaatggagc
    10381 atatcgttgg ataaagctat accgatacgt tcggctttaa cattggtttg gggatgatag
    10441 cctgctgcgt tataaaacat cataaagcgg gctcccaggc ttttcttttc atcacggtaa
    10501 accaaacttt tgtattgcgt gattgtttca aaccactggg cgtcttcgtc tttgggagac
    10561 atcaaaggct gtggaaaagt tttccattcg tgggcggtac ttatatctcc cttagtataa
    10621 gccagtccga tttgcaaggt tcccagctcg taaccgggag aattgcctcc gatgtaagtg
    10681 atccagtttt tcccctggta ttttcctatt ttataggagc ctccccattc catgtcctgc
    10741 aaagccatgt agccaccgcg ttgattactg tcccaagtgt tctcctgaaa agaaaggatg
    10801 cgtcccaggg ttttccagtt caataaatcg ttgctttcgg ccaaccaggt ttcgtagccg
    10861 cgcccgtctc tcccgccctt tccgtcgtaa accagatagg tcatatacca tttcttaccc
    10921 ttacgataca ctgtaggaca atccatttta tggaaattgt cgccgggagc cagtaccaaa
    10981 ccgtacttat gaggggtttt tactttatga tatacctctt ccatcacctg ttcaggcaca
    11041 ggattgggat tagtctttgc agttgccgtg aatataagag aaataagcac gacaaaactc
    11101 atcatccttc tcataataaa attataatcg ctttagaaat gaacaaactt aacacttta
//
LOCUS       DAOSTD010000062        11045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_5812_length_11045_cov_13.141583, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000062 DAOSTD010000000
VERSION     DAOSTD010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11045
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5812_length_11045_cov_13.141583"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="tilS"
                     /locus_tag="PLI37_05340"
     CDS             complement(<1..663)
                     /gene="tilS"
                     /locus_tag="PLI37_05340"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOQ58464.1"
                     /translation="MDLLNKVQSYIEEHRLLDAKDKVLVGFSGGADSLALLDVLLNLG
                     YNCVAVHMNFQLRGEESFRDEAFVADFCREHSVPLYVERVDTNTYAKNNSLSIEMAAR
                     KLRYDYFHRMLPKTGADKIAVGHHIDDSIETFFINLLRGSGIRGLKGISPVQGKVVRP
                     LLCADRQEILSWLEHKKLSFVTDSSNEGIVYRRNKIRHQVLPLLQSIEPSAAAAIFRS
                     MQH"
     gene            779..1798
                     /gene="pheS"
                     /locus_tag="PLI37_05345"
     CDS             779..1798
                     /gene="pheS"
                     /locus_tag="PLI37_05345"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOQ58465.1"
                     /translation="MIERIETLSDQVDTWSAQDLKELDDLRVKYLSKKGEISQLFNDF
                     RSVPGEQKRLVGQKLNELKDKAQQRLSELKELLDSQKQSNRNYDLSRSAYPYPLGTRH
                     PLSIVRNNIIRIFSMLGFTIAEGPEIEDDWHVFSSLNFPPEHPARDMQDTFFIEKNPD
                     LVLRTHTSSVQTRVMEKTSPPIRIICPGRVYRNEAISYRAHCFFHQVEALYIDKDVSF
                     ADLRQLLEYFCKEMFGEDTKIRLRPSYFPFTEPSAELDISCNICHGKGCPFCKHTGWV
                     EILGCGMVDPNVLENCGIDSSIYSGYALGMGIERIANLKYQIKDLRMFSENDLRFLEQ
                     FRAAH"
     gene            1798..2436
                     /gene="nth"
                     /locus_tag="PLI37_05350"
     CDS             1798..2436
                     /gene="nth"
                     /locus_tag="PLI37_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOQ58466.1"
                     /translation="MTLQERYKQVIEWFSANMPEARSELDFSNPYELLVAVILSAQCT
                     DKRVNTITPAFFEAFPDAETLSQSSVEEVFEYIRTCSYPNNKAKHLVGMAQALCREFN
                     GQVPSTMDELLSLPGVGRKTAGVVLVCAFNKAAMPVDTHVFRVANRLGLTPGAKTPEQ
                     SEVLLMKHFPEHLLGVAHHWLILHGRYVCQARRPKCLDCGLTSCCEYFNRNK"
     gene            complement(2538..4013)
                     /locus_tag="PLI37_05355"
     CDS             complement(2538..4013)
                     /locus_tag="PLI37_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOQ58467.1"
                     /translation="MKIYRIILVSLFGLLIHSAVFANYNVKDFGAKGDDKTIDSPAIN
                     RAIEAASQAGGGTVFFPAGTYLSYSIRLSNNIHLYLDEGAVLKAARPSAEEGYDPAEP
                     NPHDAYQDFGHSHWQNSLIWGIGLKNIRISGFGTIYGYGLSREESRLPGAANKAIALK
                     ECINVDIQDITMIRCGHFALLATGVENLGIRGVKIDTNRDGLDIDACRNVRISDCTVN
                     APWDDAIVLKASYALGYYKDTENVYISNCMVSGYDQGSMIDGSYQTDEPQAPDQDFTT
                     GRIKFGTESSGGFRNITISNCVFDRCRGLALETVDGGIMEDIVISNITMRNIVNPAIF
                     LRLGARMRSPQGRAVGSMRRISISKLNCFNIDSRFACILISGIPQAKIEEVSISDVRI
                     VQRGGGNKEWAGIEPPERERSYPEPSMFGYLPSSTLFLRHVNKLTIRNLDVSFIEADP
                     RPALYLQDVHNSSFHNLNIPLAEGQESSIQKEVSGLVFTSD"
     gene            complement(4010..>4675)
                     /locus_tag="PLI37_05360"
     CDS             complement(4010..>4675)
                     /locus_tag="PLI37_05360"
                     /inference="COORDINATES: protein motif:HMM:NF025965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4450 domain-containing protein"
                     /protein_id="HOQ58468.1"
                     /translation="VGILAYGAGDFGFFMPETAGYLKLGLMSEDKSLWLSDARKANIY
                     PTSKGWIMEFQDPFPGTNALRFELRPLQHSNGFVVELKTSQLPGNLSLVWTYGGCSGS
                     KDPAERELFLRPEQCPNNVFSRENNTFTVYYGLSTSLRVMMGVAPMDTETRIGNARSM
                     DNPMMLWNSGKKTDTPVMVARNPLRANSTYYYCIYRQNRKSDYNAFMLPALFQESDKN
                     LQP"
     assembly_gap    4676..4775
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4776..6895)
                     /locus_tag="PLI37_05365"
     CDS             complement(<4776..6895)
                     /locus_tag="PLI37_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ58469.1"
                     /translation="MNRLKRMLGLLMLALVCSSLQAERLTVSMDKNWEFCLKDLPTDS
                     VFIVQQGWRQLDLPHDWSIEGKYDKDHPSGRGGGYLPTGTAWYRKEFLLPAACEGRRV
                     FIEFDGVMANSEVYINGKLLGKRPNGYVSFSYDMSEHLRYGGNNQLSVRVDNSIQPAS
                     RWYTGSGINRHVRMSIKSPVYLPQWGVFVRSSKVSSERAELDVQIDVRNDGTERADVQ
                     IVSVLRDASGKEVRRSNSNLKALGAGDSGRVDQTLTVNKPRLWSPEDPALYTLEVSLY
                     KGNELLDTEIVTTGIRSIRFDPAQGFFLNGKNVKMYGVCLHSDGGAVGTAVPLSLWEY
                     RLEKLRDLGVNAIRLAHNPVAPEFLDLCDRMGFLVMNESFDTWQAAKNHAEKGYNLFF
                     KDWWYADTRDQVLRDRNHPSVVIFSVGNEIRDNLNNPEGFAKYKNQHDCVHKYDGSRP
                     VTMALFRPNSSGVYQNGFAEMMDVVGQNYRVEELVAYHEAHPEKAVIGTENIHDIASW
                     LVLRDKPYMSGQFLWTGIDYLGEAFWPNVMYTISLLDITAEPKPQGLQRKSWWSTEPM
                     VAMVRKSDNNGAGHWVSDWTPIDLDTYDQAEIEVYSNCEEVEILLNGVSQGRKTMDKN
                     AAPAAYRLTFRPGECKVLGYNQGKKVAETSYHTAGDPDKIVLTTSKQVLNNDWDEVAV
                     VRATVVDEKGIPCVTANHLIRFEV"
     gene            complement(6905..9055)
                     /locus_tag="PLI37_05370"
     CDS             complement(6905..9055)
                     /locus_tag="PLI37_05370"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOQ58470.1"
                     /translation="MKKIIFNRQTLPYLITCFFVLSPVLQVQAKSRKTVQNDFNTPLH
                     LLQADYKFPYGIPQQKDIQSVLERVLVFLEESTPTQVLDGKTQQVLSDYSRIDADARL
                     QQGAFRLASYEWGVTYMGMLSAGQALSNQRFTDYTLDRFRFLAETAPYFRQQMEAGRP
                     MDSQMRQMLQPAALDDCGSMCAALIKAAPLMPDTDLKPLINNYVDYIMRKQSRLSDGT
                     LSRNRPFFNSVWLDDLFMSLPALVQMGRYGSDKRYYDEACRQFKLFAEKMFVPGQGLF
                     RHAWVQGMDSHPDFFWGRANGWALMTLVEILEVLPESHPQRAYLLDMLRAHIKALAAL
                     QSPEGLWHQLLDRNDTYLETSASAIFCYAFAKAINRGWVDAQAYGPAVLLAWNAVAAK
                     VDAQGMVQGTCVGTGMAFDPAFYAHRPVSPYAAHGYGPVLLAGAEVLRLLENFHPKMN
                     DSAVQFYTHHFNTTAPIFEERIENNHGLKVPGYSRRGRRPVVFTIGDSTVRNSDGSGR
                     NGQWGWGNFLGDFLNPDSFSVENHALGGRSSRTFIEEGLWQRVLDVVEEGDYVLMQFG
                     HNDGGEPHRGRARATLKGNGDQAESFVMESTGDTILVHTYGWYMRRYIQDVLDKKAVP
                     VVCSLIPRNIWQDGKVGRNDDTYALWAQQAAESIEGAYFIDLNKLICDKYDVLGKNFV
                     NTLYYGDHTHTSELGAKMNAAIVAEEFKRLIQEK"
     gene            9188..10585
                     /locus_tag="PLI37_05375"
     CDS             9188..10585
                     /locus_tag="PLI37_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58471.1"
                     /translation="MKRLLLFCVVLLTLSGCRNPQAGLLFTKEAPDIFPDYQFVHIPW
                     NIAPLNFIINNQGNKAIVLIEGPETTLRVKSDRKVQFPLRKWKKFLLKNKGKTLEVKL
                     RLKQEKSWVEYPAFEWYVTREAVDPYLVYQRIRPTPTVWANQGIYQRNLENFDEDCLL
                     DNSFTDADGLNTFAFCAGRPDRLLFHKRRPSAQTYLLVNGEKTELSQQSFNLMAYPSW
                     HPSGQFVAFAINHSDLPFYGNIGLPAQLYNPASDILVYDVVNKQLITGSELISKDSYE
                     TFPAFSADGRSLYFCTATQTESTQKGDISYDLCRLDFYPEDRRFGSRVDTLVRAERIG
                     RSISFPKPSPDGRFILLTLHSGGRFNLWGQDSDLFYYLLKDELHGVKSCYASNSQYAE
                     DYASWSSNGAWFVFGSRRLNGLSSQLYLSYIDEKERPRKAVLLPIKDPDSYRNPDQIS
                     CHLPELLLRKAFRME"
     gene            complement(10588..>11045)
                     /locus_tag="PLI37_05380"
     CDS             complement(10588..>11045)
                     /locus_tag="PLI37_05380"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOQ58472.1"
                     /translation="FAHVDAWQWNPDLIWFDNLRSVKTPNWYVQQLYGHYKGTGVLRS
                     ASGKEALSGQNNLYASAVRDAGKKEIILKIGNSSNQPQRMKIELSGLKKFNPDAKWIT
                     MQANDLNVVNTLDEPFNIVPGEASFKLTGNVFEFEVKAQSFNVLVIAEE"
BASE COUNT         2730 a         2808 c         2614 g         2793 t
ORIGIN      
        1 gtgctgcata cttcggaata tagccgcggc agccgaaggt tctatgcttt ggagcaaagg
       61 aagcacctga tggcgtattt tgttgcgccg gtaaactatt ccctcgttgc tgctgtcggt
      121 gacaaacgag agttttttat gttccagcca cgacaaaatc tcctgcctgt ctgcgcaaag
      181 caagggtctg acaaccttgc cctgaacggg gctgatgccc tttagtccgc gaattccgct
      241 gccacgcaaa agattgataa aaaaggtctc tatgctatcg tcgatgtgat gccctacggc
      301 tattttgtcg gctcccgttt tgggcaacat ccggtggaaa tagtcgtagc gcagtttccg
      361 ggcagccatt tcgatggaaa gagagttgtt ttttgcgtaa gtattggtat cgacacgctc
      421 tacatacaaa ggaacggaat gctcgcggca gaaatccgcg acaaaggctt cgtcacggaa
      481 ggattcctct cctctaagtt gaaaattcat atgtacagcc acacagttgt agcccaggtt
      541 aagcagcaca tcgagcaagg caagcgaatc cgctcccccg ctgaatccca ccaggacctt
      601 gtctttggca tccaataaac ggtgttcttc aatatacgat tgaaccttgt tcagcaagtc
      661 cataataagc tgccttttct gtacgggcaa aggtagaaaa aacatccgat atgctctttg
      721 ccttacccaa gtatgaaaat aaactcgtac atttgcgggt caaaaatttt gcatatctat
      781 gattgaacgt atcgaaacac tctcggacca agtcgatacc tggtcggctc aggacctgaa
      841 agaactcgat gatttgcggg tcaaatacct cagcaaaaaa ggggaaatca gtcaattgtt
      901 caacgacttc agatcggtgc ccggcgaaca aaaaagactg gtcggacaaa agctgaacga
      961 gctcaaagat aaagcacaac aacgtttgag tgagctaaag gagctgctcg acagtcaaaa
     1021 acaaagcaac cggaactacg atttaagtcg cagcgcctat ccctacccgc tggggacacg
     1081 tcatcctttg tcaattgtaa ggaacaatat aatacgtatt ttctcgatgc tgggctttac
     1141 catagccgaa ggccccgaaa tcgaagacga ctggcatgtg ttcagctcgc tgaatttccc
     1201 gcccgaacac cctgccagag atatgcagga tacttttttt atagaaaaaa atcccgatct
     1261 ggttttgcgc actcatactt cgtcggtaca aaccagggtg atggaaaaaa ccagccctcc
     1321 cattcgcatc atctgcccgg gcagggtgta ccgcaacgaa gctatttcct acagggcgca
     1381 ttgtttcttt catcaagtcg aagcgctcta catagataaa gacgtctctt ttgccgatct
     1441 gaggcaattg ctcgaatact tttgcaagga aatgttcgga gaagatacca aaatccgcct
     1501 ccgcccctcc tacttcccct ttacggaacc gtcggccgaa ctggatattt cctgcaacat
     1561 ctgccacggc aaaggttgtc ccttttgcaa gcacaccggt tgggtggaaa ttttgggctg
     1621 cggcatggtc gatcccaacg ttctcgaaaa ctgcggaatc gacagctcca tctacagcgg
     1681 ctatgccttg ggtatgggca ttgaacgcat cgccaacctc aaataccaga tcaaagattt
     1741 acgtatgttc tccgaaaacg acctccgttt tctggagcaa ttccgggctg cccactgatg
     1801 actcttcagg aacgttataa gcaggtaata gagtggtttt cggcaaacat gcccgaggct
     1861 cgcagcgaat tggacttcag caatccttac gaattactgg tggctgtcat tttgtcggct
     1921 cagtgtaccg ataaaagagt gaacacgatc actccggcct tttttgaagc cttccctgac
     1981 gcggaaacac tgtctcaaag cagtgtcgaa gaggtttttg aatacattcg gacctgctcc
     2041 tatcccaaca acaaggccaa acatctggtg ggcatggctc aagctttatg ccgggaattc
     2101 aacggacaag tgccttcgac tatggacgaa ttgctgagtc tgccgggcgt aggccgcaaa
     2161 acagccggcg tggtattggt ttgtgctttc aacaaggcag ctatgcctgt agacacccat
     2221 gtatttaggg ttgccaaccg cctgggactc acacccggcg ccaaaactcc ggaacaaagt
     2281 gaagtactcc tgatgaaaca ctttcccgaa cacttgcttg gggtagctca tcattggctt
     2341 atcctgcacg gacgttacgt ctgccaggcc cgcaggccca aatgcctcga ttgcggcctt
     2401 acttcctgtt gtgagtattt caaccgaaat aaataatacg gccattccgg tcgtcgaatc
     2461 acggtaaagg gaaaaaacag cggctattca ggtgctttcc ggcggtctcc ggccaacagg
     2521 tatgccggcg gtctgaccta atccgaagta aataccagcc cgctgacctc tttttgaatg
     2581 ctgctctcct gcccttccgc cagggggata ttcaaattgt gaaagctgga attgtgcacg
     2641 tcctgtagat acaaagccgg gcgtgggtct gcttcaataa agcttacatc aagattacgg
     2701 atagtcaact tattgacgtg acgcaaaaac aaagtcgagg agggcaaata tccaaacata
     2761 gaaggctcag gataagatct ttctctttcg ggcggttcta taccggccca ttccttgttg
     2821 cctccccctc tctgaacgat acggacatcg ctgatgctga cctcttcgat tttggcctga
     2881 ggtatgccgc tgatcaaaat gcaggcaaag cgggaatcga tgttaaaaca gttcagctta
     2941 ctgatactga tgcgcctcat gctgccgact gccctgccct ggggactgcg catccttgcg
     3001 cccagacgca aaaagatggc cggattgaca atattacgca tggtgatatt tgaaatcaca
     3061 atatcttcca ttattccgcc gtctacggtt tcgagagcca ggccacgaca acgatcgaaa
     3121 acgcaattac tgatggtgat attacggaag cctccgctgg attcggtacc gaacttgatg
     3181 cgtccggtgg taaaatcctg atccggagcc tgaggttcgt ccgtctgata actgccgtct
     3241 atcatactgc cctgatcgta accgctgacc atgcaattgc tgatgtacac gttctcggtg
     3301 tctttgtaat aaccgagcgc atagctggcc ttgagcacta tggcatcgtc ccagggagca
     3361 ttgacagtgc aatcgctgat acggacattg cggcaggcgt cgatatcgag accgtcgcgg
     3421 ttggtatcaa tcttgactcc cctgatgcct aagttttcga ccccggtggc caggagggca
     3481 aaatgcccgc aacgaatcat ggtgatgtct tgaatatcca cattaataca ttctttgaga
     3541 gcgatggcct tgttggcggc tccgggcaaa cggctttctt cgcggctcag tccgtaaccg
     3601 taaatagtac cgaagccgct gatacggata ttcttcaatc ctatacccca aattaggctg
     3661 ttctgccagt ggctgtgtcc aaaatcctga taagcgtcgt gaggattggg ttctgcgggg
     3721 tcataaccct cttcggccga aggacgggct gctttcaata ctgctccttc gtcgagatac
     3781 aagtggatat tgttggagag ccggatagaa tatgaaagat aagtgccggc aggaaaaaag
     3841 acggtacccc ctcccgcctg tgaggcagcc tcgattgccc ggttgatggc cggagaatct
     3901 atggttttat cgtcgccttt ggcaccaaaa tcctttacgt tataattggc gaaaacagca
     3961 gaatggatca aaagaccaaa cagtgagact aagatgattc gataaatttt catggttgca
     4021 ggtttttgtc tgattcttga aacagggccg gcaacataaa ggcgttgtaa tcactttttc
     4081 tgttttgccg gtaaatgcaa taataatagg tagaattggc tctcagcggg ttgcgggcca
     4141 ccataacggg agtatcggtt ttcttacctg aattccacag catcatcgga ttatccatag
     4201 atcgggcatt tccgatacgg gtttcggtat ccataggcgc cacgcccatc atcaccctga
     4261 gactggtgct taagccgtaa taaacggtga aggtattgtt ttcacgactg aatacattgt
     4321 tgggacattg ttcaggccga agaaaaagtt ctctttcggc cggatctttc gagccggaac
     4381 aacctccgta agtccagact aaactgaggt tcccgggtag ctgagaggtc ttgagttcta
     4441 caacaaagcc gttgctgtgt tgcagaggtc tcagttcaaa acgcagggca ttggtgccgg
     4501 ggaaaggatc ttgaaattcc atgatccagc ctttggaagt gggataaatg ttcgccttgc
     4561 gagcatcact caaccagaga gatttgtctt ccgacatcag gcctaactta agataaccgg
     4621 ccgtttcggg cataaaaaaa ccaaaatcgc cggctccgta agccagtatc ccgacnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgcac ttcgaagcgg atgaggtgat
     4801 tggctgtaac acagggtatg cccttttcgt ccactacggt agccctgacc acggccactt
     4861 cgtcccagtc gttgtttaga acctgtttgc tggtcgtcag aacgatttta tcgggatcgc
     4921 cggccgtgtg ataagaagtt tcggccactt ttttcccctg attatatccc aatactttgc
     4981 attctccggg acgaaaagtc aagcggtaag cggcaggcgc agcgttttta tccattgttt
     5041 tcctgccctg agagactccg ttgagcagaa tttccacctc ttcgcaatta gaatatacct
     5101 ctatttcggc ctgatcgtag gtatccaaat caatgggtgt ccaatcggag acccaatggc
     5161 cggccccgtt attgtccgat ttgcgaacca tggctaccat gggctcggtg ctccaccaac
     5221 ttttgcgttg cagcccctgt ggtttgggtt cggcggtaat atcgagcagc gaaatggtgt
     5281 acatcacgtt cggccagaag gcttcgccca ggtaatcgat acccgtccac aaaaattgcc
     5341 cgctcatata gggcttatcc ctcaacacca accaggaagc tatgtcgtgg atgttttcgg
     5401 ttccaatcac ggctttttcg ggatgtgctt catgataggc caccaattct tccacccggt
     5461 agttttgtcc taccacatcc atcatttcgg caaagccgtt ttgatacaca cccgaactgt
     5521 tgggacggaa caatgccata gtcaccggcc tgctgccatc gtacttgtgg acgcaatcgt
     5581 gctggttttt gtatttggca aaaccttcag gattattgag gttgtcgcgg atttcgttgc
     5641 ccacactgaa aatcaccacc gacggatggt tgcggtcgcg cagtacctga tcgcgggtat
     5701 cggcatacca ccagtcttta aagaaaagat tatagccttt ttcggcgtga ttcttggctg
     5761 cctgccaggt atcgaacgat tcgttcatca ccaggaagcc cattctgtcg cataaatcca
     5821 aaaattccgg agccaccgga ttatgtgcca ggcggatggc attcacgccc agatcgcgga
     5881 gtttttcgag ccggtattcc cataaactca gcggaacggc cgtgcccacg gctcctccat
     5941 cggaatgcag acaaacccca tacattttga cattttttcc gttgagaaag aagccttgtg
     6001 ccggatcgaa acggatactg cgtattccgg tcgttactat ttcggtatcg agcaattcat
     6061 tgcccttata caaagacact tcgagggtat aaagcgccgg atcctccgga ctccaaagcc
     6121 tgggcttatt tactgtcagg gtctgatcca cacggcccga atctccggct ccgagggctt
     6181 tcagattact gttgctcctg cggacttcct tgcccgaagc gtctctgagt acgctcacga
     6241 tctgtacgtc tgctctttct gttccgtcgt tccgaacgtc gatctgcaca tcgagttctg
     6301 cccgttccga gctgactttg gaactgcgta caaacacacc ccattgcggc agatacacgg
     6361 gcgactttat agacattctg acgtgacggt tgatgcccga accggtgtac cagcgcgaag
     6421 ccggttgtat ggaattgtcc acccttacac tcaattggtt gtttccgcca taacggaggt
     6481 gttcgctcat atcgtaagaa aaactcacat aaccgttggg gcgttttccc aataatttcc
     6541 cgttgatata aacttcgctg ttggccatga ctccgtcgaa ttcaataaac acccgacgtc
     6601 cctcacaggc agcgggcaac agaaattctt tgcggtacca ggctgtcccg gtaggtaaat
     6661 aaccacctcc cctgccgctg ggatggtctt tgtcgtattt gccttcgatg ctccaatcgt
     6721 ggggcaaatc cagttgacgc cagccttgtt gcacaataaa taccgaatcg gtcggcaggt
     6781 ctttcaggca aaattcccag tttttgtcca tggatacggt taaacgttcc gcttgcagag
     6841 aactgcatac aagcgccaac atcaacaatc caagcattcg ttttaatctg ttcattgttc
     6901 tgtattattt ttcttgtatt aatcgtttga attcttctgc cacaatggca gcgttcattt
     6961 tggctcccaa ctcggaggta tgggtatggt ctccgtaata caaagtattg acaaagtttt
     7021 tgcccaaaac gtcgtacttg tcgcaaatca atttgtttaa atcgataaaa taggcgcctt
     7081 ctatgctctc ggctgcctgt tgggcccaaa gggcgtaagt atcatcgtta cggcccactt
     7141 tgccgtcttg ccaaatattg cggggaatca ggctgcaaac caccggaaca gcctttttgt
     7201 cgagaacatc ctgaatgtaa cgacgcatat accaaccgta agtgtgtacc aaaatggtgt
     7261 cgccggtgct ttccatcaca aaagactcag cctgatcgcc gttgcctttg agggtggccc
     7321 gggcacggcc gcggtgaggt tcaccaccgt cgttgtgtcc gaattgcatc agtacgtaat
     7381 ctccttcctc taccacatcg agcactcttt gccatagacc ttcttcaata aaagttctgc
     7441 tgctacggcc gcccagggcg tgattctcga ccgagaagga atcgggattc aaaaagtctc
     7501 ccaaaaagtt gccccaaccc cattgtccgt tgcggccgct gccgtcggaa ttgcgcacag
     7561 tggaatcacc aatggtaaaa accaccggcc gccggcctct gcggctgtaa ccgggcactt
     7621 taagtccgtg attgttttcg attctttctt cgaaaatggg agccgtggtg ttgaagtgat
     7681 gagtataaaa ctgtacggcc gaatcgttca ttttgggatg aaagttctcc aacagcctca
     7741 acacctcggc accggccaac aagacggggc cgtaaccgtg agccgcgtag ggactgacgg
     7801 gtctgtgagc ataaaaagcc ggatcaaaag ccataccggt tccaacgcag gtgccctgca
     7861 ccatgccctg cgcatctacc ttagccgcaa cggcattcca ggccaacaaa acagccggac
     7921 cgtaagcctg cgcatctacc cagcctctgt tgatggcttt ggcaaaagca taacaaaata
     7981 tggccgaagc tgaagtttcc aggtaagtat cgtttctgtc gagcagttga tgccataagc
     8041 cttcgggaga ctgcagcgcc gccagagcct tgatgtgagc tctgagcata tccaaaagat
     8101 aagcccgctg aggatgtgat tcgggtaaaa cctcgaggat ctcgaccaaa gtcatcaaag
     8161 cccagccgtt ggccctgccc caaaaaaaat cgggatgact gtccatgcct tgtacccagg
     8221 catgcctgaa taaaccttgc ccgggcacaa acattttttc ggcaaaaagt ttaaactgac
     8281 ggcaggcctc gtcgtaataa cgcttgtcgg agccgtaccg gcccatttgc accaaagcag
     8341 gcagactcat aaacaaatcg tcgagccaga ctgaattgaa aaaagggcgg ttgcgcgaca
     8401 aagttccgtc ggacagacgg gactgcttgc gcataatata atcgacgtag ttgtttatca
     8461 aaggcttcag gtcggtatcc ggcataagcg gagctgcttt tatcagggcg gcacacatgg
     8521 agccgcaatc gtcgagcgcg gcgggctgaa gcatttgtct catctggctg tccatcggcc
     8581 ggccggcttc catctgttgc ctaaaataag gagccgtttc ggccagaaaa cggaaacgat
     8641 ccagcgtata atcagtaaaa cgctgattac tcaaggcttg tccggccgac aacataccca
     8701 tataagtgac tccccattcg taactggcca atcgaaaggc gccctgctgc agcctggcgt
     8761 ccgcatcaat ccggctgtaa tcgctcagga cctgctgagt tttgccatcc agcacttgcg
     8821 tcggtgtgga ttcttcgaga aatacgagta cgcgctccag caccgactga atgtctttct
     8881 gttgcggaat gccgtaagga aacttgtaat cggcttgcaa taaatgcaaa ggagtgttga
     8941 agtcgttctg gactgttttg cgacttttag cctgaacttg aagcaccggg ctcaatacaa
     9001 aaaaacaagt gataagataa ggcaaagtct gcctgttaaa aatgattttt ttcatggcgt
     9061 ttttcttgat gtctacaaaa atagcttatc cggcgcgtag atgtatgctt aatttgatat
     9121 tttatttgtt aaaactgagc ctctaagcgg caattttgag tacttttgag ttctttagct
     9181 cctgtctatg aaaagacttc tcctgttttg cgttgtatta ttgacgttat ccggttgtcg
     9241 taacccacaa gccggcctgt tattcaccaa ggaggctccg gatattttcc ccgattatca
     9301 atttgtacat attccctgga atattgctcc gctgaatttt attatcaata accagggcaa
     9361 caaagcgatt gtcctgatcg aaggaccgga gacaacgctt cgggtaaaga gcgacaggaa
     9421 agtgcaattt cctctgcgaa aatggaaaaa attcctgttg aaaaacaaag gaaaaaccct
     9481 ggaggtaaaa ctgcgcctga aacaagagaa aagctgggtg gaataccctg cttttgaatg
     9541 gtatgtcacc cgggaggctg tagatcccta cctggtgtat caacgcatac ggcccacccc
     9601 aactgtgtgg gccaatcagg gcatttacca acgcaatctg gagaatttcg acgaagattg
     9661 cctgttggac aacagtttta ccgacgccga cggcctgaat acttttgcct tctgtgccgg
     9721 tcgtcccgat cgactgcttt ttcataaacg aaggccctcg gcacaaactt acttgttggt
     9781 gaatggagaa aaaacagagc tcagtcaaca aagcttcaac ctcatggcct atccgtcctg
     9841 gcatccctcc ggccagtttg tggcctttgc catcaatcat tccgatcttc ccttttacgg
     9901 aaacatcggt ttgcccgcac aactgtacaa tcctgcttcc gatattttag tttatgatgt
     9961 ggtaaacaaa cagttaatca ccggatctga gctgatttcc aaggatagtt acgagacttt
    10021 tccggcattt tcggccgacg gcaggagcct gtatttttgt acggcaaccc aaacggaatc
    10081 aacacaaaag ggagacatta gttacgacct ctgccgtctg gatttctatc ccgaagacag
    10141 gcgctttggc agccgggtag ataccctggt cagggccgaa cgcataggca gaagtatttc
    10201 cttccccaag ccttctcccg acggacgttt tatcttgctg accctgcatt ccggcggccg
    10261 tttcaatctc tgggggcaag acagtgattt gttttattat ttgttgaagg acgaactgca
    10321 cggagtaaaa tcctgctacg ccagcaacag ccagtacgcc gaggattatg cttcctggtc
    10381 gtccaacgga gcctggtttg tatttggcag ccgcaggctg aatggcctga gttctcagtt
    10441 gtatctcagc tatatagacg aaaaagaacg acctcgtaag gcagtattat tgcccataaa
    10501 agatccggac tcctaccgga acccggatca aatctcttgt caccttccgg aactcctgct
    10561 ccgaaaagct ttccgtatgg aataaatcta ttcttcagct atcaccagca cattgaagga
    10621 ttgggctttg acttcaaact cgaaaacatt tcctgtcagc ttaaagctgg cttcgcccgg
    10681 tacgatattg aagggctcgt ccaaagtatt aaccacattc aggtcgttgg cttgcatcgt
    10741 aatccacttg gcgtcgggat tgaatttttt caggccggac agttcaatct tcatgcgctg
    10801 cggctgattg ctactattac cgatcttaag tataatttct tttttaccgg cgtctctgac
    10861 agcactggca taaaggttgt tttggccgct caaggcttcc ttgccgctgg cagagcgcaa
    10921 gactccggtg cctttgtagt ggccgtataa ttgttgcaca taccagttgg gagttttaac
    10981 gctgcgtaaa ttatcgaacc agatcaaatc gggattccat tgccaggcat ccacatgagc
    11041 aaaca
//
LOCUS       DAOSTD010000063        10808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_5966_length_10808_cov_14.395146, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000063 DAOSTD010000000
VERSION     DAOSTD010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10808
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5966_length_10808_cov_14.395146"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..2722)
                     /gene="alaS"
                     /locus_tag="PLI37_05385"
     CDS             complement(104..2722)
                     /gene="alaS"
                     /locus_tag="PLI37_05385"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOQ58473.1"
                     /translation="MLGAKEIRESFKNFFASKGHQIVPSAPMVIKDDPSLMFTNAGMN
                     QFKNIFLGQAPAKYLRVANSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYF
                     KKEAIAWAWEYLTEVLKLDASRLYATVFEGSPEEGLQRDDEAAGYWANYLPKEHIIDG
                     NKHDNFWEMGETGPCGPCSEIHIDLRSEQERAQLDGLHLVNKNHPRVVEIWNLVFMQF
                     NRKADGSLEVLPAKHIDTGMGFERLCMAVQGKTSNYDTDLFQPLIKEIGNLCDVPYGR
                     DQQTDVAMRVVADHIRTIAFSITDGQLPSNTGAGYVIRRILRRAVRYAYTFLGRRRAF
                     LYKLIPALTEVMGSAYPELKAQQNLVEKVIKEEEDAFIRTLESGIKMLEKSMEHLKAS
                     GSTKMDGKEVFSLYDTFGFPLDLTELILRENQLEADIEGFEAEMQQQKARARNAAAVK
                     AGDWVILKDIGYVFVGYDCTECDVEIVRYRQVQQKGKTFYQLVLDKTPFYAEMGGQVG
                     DSGYLEAEGERLPIIEVKKENDLSVHICTKLPADPTRVFRAVVDRERRNRISANHTAT
                     HLLHEALREVLGTHVEQKGSYVSDELLRFDFSHFQKMSEDEIRRVERLVNRKIRSCIE
                     LDEHRHTSMQEAREMGAIALFGEKYGEEVRVVRFGSSIELCGGTHASSTGRIGYFCIL
                     SESSIAAGVRRIEALTGEKAEEHIDALKDKLSQIREMFNQAPGYIDSIRKTIDENHQL
                     RKELEALMAEKVQQIKAEIKQQAVETNGFLVIRLTMEISPDQVKNIAFQLRGECADNV
                     LFVAGTSFDNKPTLSIMLGKDLVDKGLNAGKLIREAAQFIQGNGGGQAHFAMAGGKDA
                     SGLQQAVQFVVDTVTA"
     gene            2807..3784
                     /locus_tag="PLI37_05390"
     CDS             2807..3784
                     /locus_tag="PLI37_05390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ58474.1"
                     /translation="MAKKSIKYRFNEDTLSFEPIESKAKDKLKTFTLHSILGLVFGFI
                     AFIVYSNFFDSPEAKRLKEQNMELEAQYEILLRQTQEIGSLLTDLEQRDENMYRAILQ
                     AEPIPSELRRGNFERTNRYASLAKMNHNALVMMTTREVDRLSRRVYIQSKSYDELVEL
                     MRQNEEKLLCIPSIQPVLNEDLKREASGYGWRIDPIYGTRKFHKGMDFSAPVGSDVYA
                     TGNGVVVYAGYERGGYGNFIRINHGFNYETYYAHLSKILVKRGQKVVRGEVIAKVGNT
                     GKSVGPHLHYEVRLRGQPQNPVHYYFMDLSPEEYDDMLQILANTGKAMD"
     gene            3802..4176
                     /locus_tag="PLI37_05395"
     CDS             3802..4176
                     /locus_tag="PLI37_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOQ58475.1"
                     /translation="MSDSKNTGTSDSKNTILSDSKIFYSIGEVARMFDLNESTLRFWE
                     KEFDSIKPKKTTKGFRQYTKADIEDIRLVYHLVKERGMTLVGAKKVIRNNKRKLSAKV
                     ELLDRLNAIKEELVAIRDEITE"
     gene            4214..>5215
                     /locus_tag="PLI37_05400"
     CDS             4214..>5215
                     /locus_tag="PLI37_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58476.1"
                     /translation="MKKTITRLAFMLLLFTLAVAKTAAIITDPEDYVWYDTGKLGVAD
                     PSKTFPDFFEFGKCFFGISNHDDVDVTPNKLTTRGEALLNACGFYDKTDETSKVYGIT
                     MPSSSSTDVTVLTLKLPFCDSIYVEGWRTSGSRGIRVESNLSDDYGFMGKSSGYLITT
                     GLRPRSKDSVTIYIYNTTKGSPTSYGGSLFINRIKIYKDIEEGDVPVFDGVVAGWDFL
                     YRPKASGTVDLESPVSVGEGEWESQHYPLYASDLGVVRGKLSSVSPAGGMQLGNDCGV
                     QAIKFASAGCNDTVDFSLGTEHDNYFQVEMSTLGYQELSLDFDFNIRDASDSCVVAYS
                     "
     assembly_gap    5216..5315
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5316..8450
                     /locus_tag="PLI37_05405"
     CDS             <5316..8450
                     /locus_tag="PLI37_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58477.1"
                     /translation="LDLVILPALREKYNVTVFYPDLYAGITDSAAINELFAPYDLVVL
                     SEFPGGASDITKAVKFLVGNKPLLNFKAYAYTISGRWGWANPSNGLYNTEVPVETHAI
                     VGDRFIYHPIFKGLTIGEDNSIQIFDTLHLTRGKTEKDFKGLQGFVTDSYVGPEGFSL
                     ASPIGSPNVTCFHEISSNPAAKYMLLAFAAENYANIGEEGLQLVLNAVDYVASPSAFA
                     VPNFNMNATGATVETVEELRAALTYDYSALNLDEILIQVKDCLDPSGIFLLGSDGMAF
                     PQSFNDLSVKAAPGATPQFWGSFRSNNGMKVGTLRFEGLSWNGGDSALTGFGDENYQP
                     FAVLQPDSVTKAFLVTNCRFENFDYQRIFRSNACAGALINKLVFENNYFDNMGWNRPA
                     GSHGQSFIQFVNSDNYQLDSLVFHNNIVKNFHGNQLFNVPRNGTTATPDTLGNFYVGI
                     THNTFYKLGGQGNSDRNFLEYNGTLGGLNGSVNISNNLFIERWSYEKFPQGYLALPAT
                     LADGQSLEVKVLNNFFMPDTVLTPRLGGVEPLQVKGESVVVPVRNNLFAQDLGITTVF
                     EDEQALTISKGSPLYTAGTDRSPLGVTSRYVTRQHSGTLRVSNVPELKTALEIAIGGD
                     VIELENCDDPTGVYLLGSSGFAFPQTFGNLTIKAAPEQEPQLFGRLWSSNGMKLDSLI
                     VEGLHWNGGDSSYAGFNSEHYAPFQISGPDTIRQAVVVRHSTFRNFDYQPVLRTRNST
                     GSLVKEWVFTHCLFDNMGWNRKTGIKAFHFIQFEDNTQAELDRFSFTENILKNFHGSQ
                     LFNIAISGSPSADSLVVINITNNTFYRLGGNSTDARQFLGFNRKNAFDNYININDNLF
                     IERWSTANKYPNSNLALFTPDSAQVMVLNILNNFFYPDTVQALPGTGQNLPVTEGDTE
                     RMTFARNDLFMSNLFMDKIFEDSEKLLIHVSSPLYTAGTNAGYVGALDSYTTQTGVVG
                     PRLQLAETGKLFTQQGTLFVHIQKTQNMSIYNILGKEILKRQLQEGLNAIEGLQQGQV
                     YLIKLDDRVTKVVM"
     gene            8794..>10808
                     /locus_tag="PLI37_05410"
     CDS             8794..>10808
                     /locus_tag="PLI37_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58478.1"
                     /translation="MKRPTKLLTALLALFIAFPSLKAGNMQVYTAGDLPTSAGTFVAP
                     DFFGGAGWSYRSRTMDPAKKDTAFFPNVLGVTDGVAYGIEISKTQDDPGNIFLSLNLH
                     SCDSIIVAGWGTGGRGILVTNSANTDSSWKGASSYNLIQVPVKVGTDSPVEIKITPTG
                     KDSKNSTSNGGTWITRIVVFAKEGSEVTQYYLSNPGFESPDTTLLVATSNSGIWIPKG
                     WTVEYPTFDTWDQGIVTSLNDAGGSALPLTAQEGSNFYYGRLRWATNSISLKQDLINP
                     PAGYNVVSMEVASPDGYKAQLAVSTDADTVTESPTGTGWNTIDLVFAGEEGKNATFSA
                     TASSASGAAQNRMFLDDARMVAYGTNFPPSVLDQRTALIAEANALYGDGSGEGAAALQ
                     EAINAFEAVPTTCALGPAETMMAALVALQTAGAELQAAMYDFNVANASPTNPMDLTSK
                     VVNPSANQGRTGWNWSFSSAVQNQGIGTDALFTKATPPYFEAWTPASNKPMTGRIYQI
                     ITDLPNGKYELTVAAQGKGGATDNLHVYANDALSGVFVDTVITVPFMVTAGTAEIGIE
                     AEAADVNWIAIDEIHLSYLGFDPAAMIDALNAQIAEADSLLTLKMKESVATELTNART
                     NAAAAVAASPAVKSQLEAAGAEMAAALPAANASIAGYATLKAAIEAS"
BASE COUNT         2814 a         2779 c         2533 g         2582 t
ORIGIN      
        1 ttgtgatacc aaaaatcaac aagacgtctg tatccataat cgagaggctg agcgggctcg
       61 gatttcatgg cggaaagcca ccaaccgaaa accggcttta tactcaagcg gtcactgtat
      121 ccaccacaaa ctgcaccgcc tgctgcaaac cgcttgcatc cttgcctccg gccatggcga
      181 aatgagcttg tcctccgccg ttgccttgaa tgaattgagc ggcttcacga atcagtttgc
      241 ctgcattcag acccttgtct accaagtctt tgcccagcat gatgctcaga gtgggtttgt
      301 tgtcgaagct ggtgcccgct acaaaaagaa cgttatcggc gcattctccc ctcaactgaa
      361 aagcaatatt tttaacctga tcgggcgaaa tttccatggt cagacgaatc actaagaaac
      421 cgtttgtttc gacggcttgc tgcttgattt cggctttgat ttgctgcacc ttttcggcca
      481 tcagagcctc cagttctttg cgcagttgat ggttttcgtc tatggttttg cgaatggagt
      541 cgatgtaacc gggagcctga ttaaacattt ctctgatttg tgagagtttg tctttgagcg
      601 catcgatatg ctcttcggct ttctcgcccg tcaaagcttc aattcttctg acaccggcgg
      661 caattgagct ttcgctgagt atgcaaaaat aacctatcct gccggtagaa gaagcatgcg
      721 ttcccccaca caattcgatg gaactgccaa agcgcaccac ccggacttct tcgccgtatt
      781 tttcgccaaa cagagcaatg gctcccattt cgcgggcttc ctgcatagag gtatggcgat
      841 gttcgtccag ctcaatgcag gaacgaatct tacggttgac caaacgctcc actcggcgga
      901 tttcgtcttc gctcattttt tgaaaatgcg aaaaatcgaa acgcagcagt tcgtccgaaa
      961 cataagaacc tttttgctct acgtgagtac ccaacacttc tctcagggct tcgtgcaaaa
     1021 gatgagtcgc cgtatgattg gccgaaatac ggttacgcct ttcccggtcg accactgcac
     1081 ggaaaacccg ggtcggatcg gcgggaagct tggtacaaat atgcacgctc aggtcgtttt
     1141 ctttttttac ttcgatgata ggcaggcgtt caccttcggc ctcgagataa ccggaatcgc
     1201 ccacctggcc gcccatttcg gcataaaaag gcgttttgtc caaaaccagt tgataaaatg
     1261 ttttgccttt ttgctgcacc tgacggtaac gaacaatttc gacgtcgcat tcggtacaat
     1321 cgtagcccac aaaaacataa ccgatgtctt tcagtatcac ccagtcgccg gcttttacgg
     1381 cggcggcatt gcgggctctg gccttttgtt gctgcatttc ggcctcaaaa ccttcgatgt
     1441 cggcttcgag ttgattttca cgaagtatca gttcggtcag atccagaggg aatccaaagg
     1501 tgtcgtaaag gctgaacacc tctttgccat ccatttttgt cgaaccggag gctttgaggt
     1561 gttccatact cttttcgagc attttgatgc ccgattccag agtgcgaata aaggcgtcct
     1621 cttcttcctt aatcactttt tccaccaggt tttgctgagc tttcagttcg ggataagcag
     1681 aacccattac ttcggtaagg gcgggaatca gtttatacaa aaaggcgcgg cgtctgccca
     1741 ggaaagtata ggcgtaacgc accgcacggc gcagaattcg gcgaataacg tagccggcac
     1801 cggtgttgga aggcaactgt ccgtcggtga tggaaaaggc gatggtgcgt atatggtcgg
     1861 ccaccacccg catggcaacg tcggtttgtt ggtcacggcc gtatggtaca tcgcagagat
     1921 tgccgatttc tttgatcaaa ggctgaaaca aatcggtatc gtaattggag gttttgccct
     1981 gaactgccat acacaggcgt tcgaagccca taccggtatc gatgtgtttg gccggcaaca
     2041 cctcgaggga accgtctgcc ttgcggttga attgcataaa caccaggttc cagatttcca
     2101 ccacccgggg gtgatttttg ttgaccaggt gcaatccgtc caattgagcc cgctcctgct
     2161 cggaacgtaa atctatatga atttcggaac aaggtccgca ggggccggtt tcgcccatct
     2221 cccagaaatt atcgtgctta ttgccgtcga tgatgtgttc cttgggcaaa taattagccc
     2281 aatagccggc ggcttcgtcg tctctttgca aaccttcttc ggggctgcct tcaaacacgg
     2341 ttgcgtaaag cctcgaagca tcgagtttaa gcacttcggt caagtattcc caggcccagg
     2401 ctatggcttc ttttttgaaa taatcgccga aagaccagtt gcccaacatt tcgaacatgg
     2461 tatgatgata ggtgtcgtga cccacctctt ccaggtcgtt gtgtttaccg ctcaccctca
     2521 gacatttctg cgaattggcc acccggagat atttggcggg agcctgtccc agaaaaatgt
     2581 ttttaaactg gttcattccg gcattggtaa acatcaggct gggatcgtct ttgatcacca
     2641 taggtgcgga aggtactatt tgatgcccct tggaggcaaa gaagtttttg aaagattcac
     2701 gaatctcttt tgcgccaagc ataattaatt taatataaaa acgagtgcaa aagtacggtt
     2761 ttattcttac ttttgcctct gattttaaat taaaagcaag aaattcgtgg caaaaaaaag
     2821 catcaaatac agattcaacg aagatacgct gagtttcgag cccattgaat ccaaagccaa
     2881 agacaaactt aaaactttta cgctgcacag cattctgggc ctggtctttg gatttattgc
     2941 ttttattgtt tattcgaatt tcttcgattc gcccgaagct aaacggctca aggagcaaaa
     3001 catggaactg gaagcccagt acgagatttt actcaggcaa actcaggaaa tcggaagctt
     3061 gctaaccgat ctggaacagc gggacgaaaa tatgtaccgg gctattttgc aagccgaacc
     3121 cattccttct gaattgagac ggggtaactt tgagagaacc aaccgctacg ccagtctggc
     3181 caaaatgaat cacaatgctt tggtgatgat gaccacccgc gaggttgacc ggctttcgag
     3241 gcgggtgtat attcagtcaa aatcgtacga tgagttggtt gagttgatgc gccaaaacga
     3301 agaaaaactc ttgtgcatac cctccataca accggtgttg aacgaagatc tcaaaaggga
     3361 agcctccggt tacggctggc gcatagaccc gatttacgga acccgtaaat ttcataaagg
     3421 aatggacttt tccgcccctg taggatcgga cgtttacgca accgggaacg gcgttgttgt
     3481 ttatgccggt tatgaacggg gaggctacgg caattttatc cgaattaatc acggtttcaa
     3541 ctacgaaacc tattatgctc acctcagtaa aattttagta aaaagaggac aaaaagtggt
     3601 gcggggagaa gtcattgcca aagtaggcaa taccggaaaa tccgttggac cacacctgca
     3661 ctacgaagta cgtttgcgcg ggcagcccca gaatccggtg cattattact ttatggacct
     3721 ttcgcccgaa gaatacgacg atatgttgca aatcctggcc aatacgggta aggccatgga
     3781 ctaatctaat accgagcagc catgtccgat tcaaaaaaca ccggcacatc cgattcgaaa
     3841 aacaccattc tgtccgattc caaaatcttt tattccatcg gcgaagtggc ccggatgttt
     3901 gacttgaatg aatccacttt gcgtttttgg gaaaaggaat tcgacagcat aaaacccaaa
     3961 aaaaccacca aaggcttccg tcaatacacc aaagccgaca tcgaagacat ccgccttgtc
     4021 tatcatttgg taaaagaaag aggcatgacc ctggtgggag ccaaaaaagt gatccgaaac
     4081 aacaagcgca aactctccgc caaagtggaa ctgctcgaca gactgaatgc cattaaagaa
     4141 gaattggtgg ctatcaggga tgaaataaca gaataagatt tttatacacc aactttttaa
     4201 tacctgaacg tttatgaaaa agactattac ccgattagca tttatgcttc ttctctttac
     4261 gctggcggta gctaaaactg ctgccatcat taccgatccc gaagattatg tatggtacga
     4321 taccggaaaa ttgggtgttg ccgatccctc gaaaactttc cccgacttct tcgaatttgg
     4381 aaaatgcttt tttggaattt ccaatcacga cgatgtagac gtaactccaa acaagctcac
     4441 aaccagaggc gaagccttgt tgaatgcttg cgggttttac gacaaaacag acgaaaccag
     4501 caaggtttac ggtattacga tgccttcgag ctcttcgact gatgtaacag tgttgacact
     4561 aaaattacct ttttgcgact ccatctatgt ggaaggctgg cgcaccagcg gcagcagggg
     4621 tattcgcgta gaaagcaacc tgagcgacga ttatggtttc atgggaaaat ccagcggcta
     4681 cctgataacg acgggacttc gtccccgttc caaggactcc gttaccatat acatatacaa
     4741 cacgactaaa ggttctccca cttcatacgg aggctcttta tttattaacc gcatcaaaat
     4801 atacaaagac atcgaagagg gcgacgttcc tgtctttgac ggagttgttg ccggctggga
     4861 ttttttgtac cgtcccaaag ccagtggtac ggtcgatttg gaatcgccgg tttccgttgg
     4921 tgagggcgaa tgggaatcgc aacattaccc cttgtacgct tccgatttgg gtgtggtgcg
     4981 cggcaaacta tcgagtgttt cgcccgccgg gggaatgcaa ctgggcaacg attgcggagt
     5041 gcaggccatt aagtttgctt ctgccggctg caacgatacg gtcgatttta gcctgggaac
     5101 ggaacatgat aactacttcc aggtagaaat gtccaccctg ggctatcagg aactttctct
     5161 ggactttgac ttcaacatcc gcgatgcttc agacagttgc gtggtggctt attcgnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctgga tttggtgatt ctgcccgctc
     5341 tgagagaaaa atacaatgta acggtctttt atccggatct ctatgcaggc atcaccgaca
     5401 gtgcagccat caacgaactt tttgctcctt acgacctggt ggtgctcagc gaattcccgg
     5461 gaggagcgtc cgacatcacc aaggcggtta agttcctggt gggcaacaaa cctctgctca
     5521 acttcaaggc ttacgcttat accatcagcg gaagatgggg atgggctaat cccagcaacg
     5581 gactttataa taccgaagtt cctgtggaaa ctcatgccat cgtaggggac aggtttatct
     5641 atcatcccat ctttaagggg ttgaccatag gtgaagacaa ctctatacaa atctttgata
     5701 ccctgcatct gacgagaggc aaaaccgaaa aagactttaa agggctgcag ggttttgtta
     5761 ccgatagcta tgtgggaccc gaagggttct ccctggccag ccccatcggg agccccaatg
     5821 tgacctgctt ccacgagatc agctccaacc ccgcggctaa atacatgcta ctggcctttg
     5881 ccgcagagaa ctacgccaac atcggggagg aaggactcca actggtgctc aatgccgtag
     5941 actatgtggc ttcgccctcg gcctttgccg tgcccaactt caacatgaac gccacgggag
     6001 ccaccgtgga aacggtagaa gaattgcgtg ctgccctcac ctacgactac agtgccctga
     6061 acctggacga aatactcatt caggtgaaag actgtctcga tccgtcgggc atcttcctgc
     6121 tgggcagcga cggcatggcc ttcccacaga gctttaacga cctgagcgtc aaagccgctc
     6181 ccggggctac tcctcaattc tggggctcgt tccgctccaa caacggtatg aaggtaggta
     6241 ccctgcgctt cgaagggctc tcgtggaacg gaggcgactc cgctctgaca ggcttcggcg
     6301 acgaaaacta ccaacctttt gccgtcttgc aacccgactc tgtgacgaag gcattcctgg
     6361 tgaccaactg tcgtttcgaa aacttcgact accagagaat cttccgctcc aatgcctgtg
     6421 ccggtgcgct gatcaacaag ctggtatttg aaaacaatta cttcgacaat atgggctgga
     6481 accgtccggc cggcagccac ggacaaagct ttatacagtt tgtcaactcc gacaactacc
     6541 agttggacag cctggtgttc cacaacaaca ttgtcaaaaa cttccacggt aatcagctct
     6601 tcaacgtccc aaggaacggt accacggcga cccccgatac gttgggcaac ttctatgtgg
     6661 gcatcaccca caacaccttc tataaattgg gcggacaagg caacagcgac agaaacttcc
     6721 tggaatacaa cggtacgctg ggaggactca acggcagtgt aaacatctcc aacaacctct
     6781 ttattgagcg ctggagttac gagaagttcc cccagggcta cctggcacta cccgctaccc
     6841 tggccgacgg acaaagcctg gaagttaagg tgctgaacaa cttctttatg cccgatacgg
     6901 tactgacacc tcgcctggga ggcgttgagc ccctgcaggt caagggagaa agtgtggtgg
     6961 ttccggtccg caacaatctc tttgctcaag acctgggcat taccactgtc tttgaagacg
     7021 agcaagccct gaccatttcc aagggatcgc ccttgtatac ggccggtacc gataggtcgc
     7081 ccctaggtgt aacaagccgc tacgtgactc gtcagcatag cggtaccctg cgcgtgagca
     7141 acgttcctga gctgaaaacg gctctggaaa tcgccatcgg aggtgacgtt atagagctgg
     7201 aaaactgcga cgatcccacg ggtgtttacc tgctgggcag ttccggcttt gccttccctc
     7261 aaacgtttgg aaaccttacg atcaaagctg caccggagca agaaccgcaa ctctttggca
     7321 ggttgtggtc gtccaacggc atgaagctcg actccctgat cgtcgaaggg ctgcactgga
     7381 acggtggaga ctcctcctac gccggattca acagcgaaca ttacgcgccc ttccagatct
     7441 ccggccccga caccatacgc caagccgtag tggtaaggca ttccacgttc aggaacttcg
     7501 actaccagcc ggtgctcaga accagaaact ccacgggttc gctcgtcaaa gagtgggtct
     7561 ttacccattg tttgtttgac aacatgggat ggaacaggaa aacgggcatc aaggctttcc
     7621 acttcattca gttcgaagac aacacccagg cggaactcga ccgcttcagc tttacggaga
     7681 acatcctgaa aaacttccac ggcagccagt tgttcaatat cgccatttcg ggatcgccct
     7741 ctgcagattc cctggtggtg atcaacatca caaacaacac tttctaccgc ctgggaggaa
     7801 actccaccga tgccaggcag ttcctgggat tcaacaggaa gaatgctttt gacaactaca
     7861 tcaacatcaa cgacaacctc tttattgaac ggtggagtac ggccaacaag taccccaaca
     7921 gcaacctggc cctctttaca cccgattcgg ctcaagttat ggtgttgaac atactcaaca
     7981 acttcttcta tcccgacacc gtgcaggcct tgcccggaac cggccagaac ctgcccgtaa
     8041 cggaagggga caccgaaagg atgacttttg ccagaaacga cctctttatg tccaacctat
     8101 tcatggacaa aatcttcgaa gacagcgaaa agctcctcat ccatgtctct tcgcccctgt
     8161 acacggcagg taccaacgca ggatacgtcg gagcattgga ttcctacacc actcaaaccg
     8221 gagtggtcgg cccaaggctg caactcgccg aaacaggcaa actctttacc caacagggaa
     8281 cactctttgt gcatattcaa aaaacacaaa acatgagcat ctacaatatc ctgggcaaag
     8341 aaatcctaaa acggcagttg caagaaggcc tcaacgccat agaaggactg caacagggac
     8401 aggtttactt aatcaaactt gacgatagag tcactaaggt ggtgatgtaa gaaacggcct
     8461 ctctcaacag gattattggc gatctttttc acaagcccgg ttccaagttc cgggcttgtt
     8521 tattaacaaa cagtttcaca ctgtcccggc ttattagtta aatttttgta attgttctgc
     8581 ttttttaaac gatttaattg tatatttggt tctgattttt gaatcaataa ggcttacagg
     8641 cagctgagaa caaaaattaa aataccgtaa attcacaacg ggctgaaact caatgttgaa
     8701 gtccgcgttt ataattgtca tcaatctatg cattcaaaaa ctgaaaaaaa tcaaaacaac
     8761 tactctacat ttttaacctt taaattaaac tttatgaaaa gacctacaaa gttattgacg
     8821 gcgctattgg cgctgtttat tgcatttcct tcgctaaaag cggggaatat gcaggtgtac
     8881 actgcggggg atcttcctac tagtgccggt acgttcgtcg cacccgactt ctttggtggt
     8941 gcaggttgga gctacagaag ccgcaccatg gatcccgcca aaaaagacac cgccttcttt
     9001 cccaatgttt tgggagtaac cgatggcgta gcctacggta tagaaatcag caaaactcag
     9061 gacgacccag gcaacatttt tctcagcctg aacctgcatt cctgcgattc aattatcgta
     9121 gcaggctggg gaacgggagg cagaggaatc ctcgttacca acagtgccaa taccgactcc
     9181 agttggaagg gagccagttc ttacaacctg attcaggttc ccgttaaagt aggaacagac
     9241 tctccggtgg aaataaaaat cactcccacc ggcaaggaca gtaaaaactc taccagcaac
     9301 ggaggtacct ggattacacg cattgttgtt ttcgccaaag aaggatcgga ggtaactcag
     9361 tattatctga gtaatcccgg atttgagtcg cccgacacta ctctattggt agcaacaagc
     9421 aacagtggaa tctggattcc caagggctgg acggtggaat accccacctt cgatacctgg
     9481 gatcagggaa tcgttacctc tctcaacgac gcaggaggca gtgcgttgcc actcaccgct
     9541 caggaaggtt ccaattttta ttatggacgc ttacgctggg ctaccaattc gattagtctg
     9601 aaacaagacc tgatcaatcc tccggccggc tacaacgtag tcagcatgga agtggcttca
     9661 ccggatggat acaaagccca gctggctgtt agcacagatg ccgacactgt aaccgaatcg
     9721 cccaccggca ccggatggaa taccatcgat ttggtttttg ccggcgaaga aggcaagaac
     9781 gccactttta gcgcaaccgc cagttctgcc tcgggtgctg cccagaaccg catgttcctg
     9841 gacgatgcca gaatggtagc ttacggaaca aactttccgc ccagtgtgct ggaccaaaga
     9901 acagctttga ttgctgaagc caacgcactc tacggagacg gctcgggcga aggagctgct
     9961 gccctgcaag aagccattaa cgcctttgaa gctgttccca ccacttgtgc tctgggcccg
    10021 gcagaaacta tgatggctgc ccttgtagca ttgcaaacgg caggcgctga actgcaagcc
    10081 gctatgtatg atttcaacgt agccaacgcc tcaccgacca atccgatgga cctgacttcc
    10141 aaagtggtca atccaagtgc caatcagggc agaacaggct ggaactggtc gttctcaagc
    10201 gcagtccaga atcaagggat cggaacagac gctttgttca ccaaagctac tcctccctat
    10261 ttcgaagcct ggactcctgc gtcaaataaa cccatgaccg gcagaatcta ccaaatcatc
    10321 acagacctgc ccaacggtaa gtatgaactg accgttgccg ctcagggtaa aggcggagca
    10381 acagacaatt tacatgttta tgccaacgac gctctctccg gagttttcgt tgataccgta
    10441 attaccgttc ctttcatggt aacggcagga acagccgaga taggtattga agccgaagct
    10501 gccgacgtca actggatagc aatagacgaa atccacttga gctacctggg ctttgatccc
    10561 gcagccatga tcgacgcgct gaacgctcaa attgcagaag ccgattcctt gttaacctta
    10621 aaaatgaaag aatcggttgc aacagagctg accaatgcca gaacaaatgc agcagctgcc
    10681 gttgcagctt ctcccgctgt taaatctcaa ctggaagcag caggagccga aatggccgcc
    10741 gcacttccgg cagccaatgc ttctattgcc ggctacgcaa cactgaaagc cgccatcgaa
    10801 gcttcgga
//
LOCUS       DAOSTD010000064        10552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_6142_length_10552_cov_16.572830, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000064 DAOSTD010000000
VERSION     DAOSTD010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10552
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6142_length_10552_cov_16.572830"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLI37_05415"
     CDS             <1..385
                     /locus_tag="PLI37_05415"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOQ58479.1"
                     /translation="FPRFFFSSLWALGIFGLFVFDFSETTKDLPLIPYADKIVHALLF
                     FIQTGLLLWELNFRSFTSTRIPIGRLFIWSVSLPFLYGVLIELIQHFLPYRGAELMDL
                     LFDLMGSLAAFSCLVLILIWRENKR"
     gene            465..2330
                     /locus_tag="PLI37_05420"
     CDS             465..2330
                     /locus_tag="PLI37_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008640450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOQ58480.1"
                     /translation="MKSLLVFLLLTAAGFNAYSSAKIWVSPEGSDSNPGTEQQAMASL
                     QGALRKAREMRRLQDPAVEKGIEIILKDGYYALPETLLIRPEDSGSRDRPTTIKAEHY
                     GKAIIGGGLKIEGWEPLKKRVKGMNKSIGSKVWVAKVPTCGGRRIDFRQFYVNGRKAG
                     PAAHSDTSCMQRILAVDYEKQEIHIPAPEQRNFVTTDGMEFFIHQMWEVAILRIKDFR
                     IKGNEAILSFHAPESRLEFEHPWPPPVVSEEGNSPFILSRAIEFLDKPGEWYHDREQA
                     LLYYLPLNGQDMQQAECIVPVLETLVSVQGSLDRPAQYIRFEGLSFAHSTWLRPSKQG
                     YVPLQAGMFLLDAYKLQQPGTHDKATLENQAWIGRQAAAVELYAVNNTVFEACRFEHL
                     GASGLDYIEASADDRIEGCVFQDIAGSAIVMGRFSEPGTETHLPYQPKDYREVSRKAL
                     ITDNYIFNCATEYWGCPGILAGYVQEVEIRHNEVCELPYTGISVGWGWTRTANCMKNN
                     RIVANRVHRFGRHNYSCGGLYTLSAQTGTLIAENYVHDIYRPEYVHDKNQGYYIYLDE
                     ASSWMTIRDNWCSLPKFGQNQPGLNHWENNGPQVDESIKLKAGIRPPWQYIKSQP"
     gene            2380..2856
                     /locus_tag="PLI37_05425"
     CDS             2380..2856
                     /locus_tag="PLI37_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HOQ58481.1"
                     /translation="MFGFGKTIEILSPIEGSSLPLSECGDEAFAEEMLGKGLAVLPAK
                     GLVVAPFHAKVEVLFDTAHALSLLSDEGVELLIHIGIDTVKLQGKHFKALVKQGDRVR
                     AGEALIEFDPAAIREEGYSTVVPVVVCNSADYAAVESFPGRKLGMLDKPFIRIRKK"
     gene            2860..4494
                     /gene="ptsP"
                     /locus_tag="PLI37_05430"
     CDS             2860..4494
                     /gene="ptsP"
                     /locus_tag="PLI37_05430"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOQ58482.1"
                     /translation="MRTGKGLGIVNGMASGKLVVVQPRTPLHLEAEKTADPRHELDMY
                     LKAREEALLQLRLLQEQSTATLGKEQAAIFEAQAMMLMDEDFEDAVSSLILKKNLSAT
                     QAVKAGGRKIAALFLGMEDERMRSKSADILDLVQRLHSILSGQTEIPVPPPEAFILLA
                     EDLSPSELIQWRSPLLKGIVLSRGSAHSHLAILAAAMKIPCLISADIEPDQARGNTRI
                     ALDATAGFFYLDPGVEFMSRFNQKISREDSNGQIPVDLRFYSPRGEEIEVLANIGSPE
                     ELDPDLQKNCQGIGLFRSEFIFMGRRELPSEEEQFELYRRLATGMKGKKVVIRTLDIG
                     ADKDAPCLNLAKEENPALGNRGIRLCLQRPELFRTQLRAVCRAAVYGKLMLLVPMLIS
                     VDELLRVKRLLLDIQQELRQEGLEIPDLPLGAMIETPASVMLCKELAAEADFLSIGTN
                     DLTQYTLAIDRQNPELASLYQQAHPALLRMLREVIRAAKAAQIPCSICGGLAQDTKHT
                     AMLIDMGFSAFSMAPVHIPGFKKFLYEHYSRSEPSL"
     gene            4504..4761
                     /locus_tag="PLI37_05435"
     CDS             4504..4761
                     /locus_tag="PLI37_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOQ58483.1"
                     /translation="MTSFTHTIADPLGIHARPAALLVKTASAFRSSIILRHGDKEISA
                     KKMIALMSLGLKQGDKLEIRIEGEDEEQAGQAMKKILEEQL"
     gene            4758..6338
                     /locus_tag="PLI37_05440"
     CDS             4758..6338
                     /locus_tag="PLI37_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HOQ58484.1"
                     /translation="MMKYLQRLGKSLMLPVACLPVAGILLGIGYWLESSLGSNIPATF
                     LLQAGGAILNHIPLLFAVGVAVGMADESNGASALAGLVSWLVITTLLSPATVQMFSGQ
                     TADPAFEKIENVFTGILSGITAAFCYNRFKNTSLPDALAFFGGKRSVPIVAAGLACLI
                     SILLFFSWPLMYNGLILFGKSILDTGPVGAGLYAFFNRLLIPLGLHHALNQVFWFDVA
                     GINDLSNFWTGQGTLGITGQYMTGFFPIMMFGLPAAALAMYHTARPERKKAVGGLMLA
                     SSLSAFLTGVTEPLEFSFMFLAPGLYVLHALLTGLTAGIVAYLPVRAGFNFSAGLVDY
                     LLSFKSPMALNPLRIIGIGLLTALVYYLVFRFTIQAFGLRTPGREEENRETPPDEEIR
                     RTPPGNESREALPGGDKDKEDGRKGKKAPAGKGKKTRAGKGKITPALGSKNSDNYRET
                     AALLIQGLGGKENIAELDNCISRLRIVIRQDETVDETILKSAAIAGIVRPGKNTIQLI
                     VGPKVQFLAKEMKQLLTK"
     gene            6351..7706
                     /locus_tag="PLI37_05445"
     CDS             6351..7706
                     /locus_tag="PLI37_05445"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOQ58485.1"
                     /translation="MTIKDIIQSRQTQFLDELFSLMRIPSVSGQQAHLQDMQDCAQRW
                     KEILLSSGVDKAEIFQTKGHPIVYGEKIIDPKAHTVLVYGHYDVMPEDPVELWTTPPF
                     EPHIENGLIKGRGADDDKGQSMIQVKAFQLALENNLLACNVKFLFEGEEEIGSIHLEE
                     FCRDHKDLLKADVILVSDTSMLGADTPSITTGLRGISYWEVEVTGAARDLHSGIFGGA
                     VPNPIQELSAIISRLIDKEGKITIPGFYDKVQEASAEERTMLAAVPHDDEAYRKSVGA
                     PGLKGEKGYSTIERTGIRPTLDVCGIWGGYTGEGVKTVLPSKAYAKISTRLVPDQDYQ
                     EIGRLFEDYIRRLAPPEVKVEVRFKHGGNPYVCSLDLPAYKAAEAAYHKVFGKRPLAI
                     RRGGSIPVIATFEKVLGIKSILMGFGLESNAIHSPNENFPLNLWLQSIEVVVEFYRLY
                     K"
     gene            7714..>10552
                     /locus_tag="PLI37_05450"
     CDS             7714..>10552
                     /locus_tag="PLI37_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOQ58486.1"
                     /translation="MNSRSLGFRSGSGFRAEFKFLFLVLPLALVFSACSGPCFRVSEN
                     GVFIRPDQGKTATRSLYLQVVDDDIIRVIASPNRKISLKAGLMCNWQDKAFSPSGRWQ
                     VFRSGDSVVLQTAALKVTALLSSGELSFSDLNGSVFLREQFGGGKSFSPFEYEGCRAY
                     SFRQVFESPPDEAFFGLGQHQSDEFNYKNKHEALYQYNTKVSVPFVVSSNNYGLLWDN
                     NSYSKWGDPRDFTDLDQFRLFDKYGKEGGLTAFYSNPGRKKELERVEKAIDYQNLNSI
                     KNFPQAFPLQNARVSWEGSIETDTAGLFRFALYYAGYVKLYLDDTLVVPERWRTAWNP
                     NTCKFSYPLQAGQKHRIRLEWQPDGGSSYIGLKALSPLSSGEEGKISFWSEMGKQMDY
                     YVVRGRNADEVVSGYRRLTGKAQVMPKWAMGFWQSRERYKTQDELLSVLREFRRRQIP
                     IDNIVQDWSYWEENAWGSHEFDAQRFPDPEQMVGDVHDLNARIMISVWPKFYINTGHY
                     KEFEQKGWIYPRAVKDSIRDWIGRGYIGSFYDAFSPGARKLFWEQMQEHLYVKGFDAW
                     WMDASEPDILSNADMDYRKQLMHPTALGPSALHFNAYGLMNAKGIYEGQRSVNDSNRV
                     FILTRSGYAGSQRFAAAVWSGDIGTRWEDMKAQISAGQNFSISGNPYWTMDNGGFCVE
                     KRYERAREGSEDLEEWRELNARWHQFGAFVPLFRSHGQYPYREIWNIAPEGHPAYQTM
                     KYYSELRYRLLPYIYTMAGRCYLDDYTIMRPLMMDFANDDRVMNIGDQYMFGSHLMVC
                     PVYEYKARRRLVYFPQNAGWYDLYSGKYYPGGQESPVEAPYERMPVFVPAGSILPTGK
                     PIQHSGEKQETLRFYVFSGQDGKACLYEDDATSYDYEKGDYARITFHYNDAQKSLSIS
                     AIEGSYRPEPTQRRFEFVLVSPQCPAGLDTEV"
BASE COUNT         2668 a         2724 c         2714 g         2446 t
ORIGIN      
        1 tttcccgcga tttttctttt catcgctgtg ggctctcggt atttttggcc ttttcgtttt
       61 cgacttttcc gaaaccacca aagacttacc tctgattccc tatgccgaca aaatcgtgca
      121 tgccctgttg tttttcatcc aaacaggcct tttgttgtgg gaactcaatt ttcgaagttt
      181 cacctccacc cgtattccca ttggccgttt atttatttgg tcggtatcgc ttccttttct
      241 atacggtgtt ttgatagagc ttattcaaca ttttttaccc taccggggag ctgaacttat
      301 ggatttgctt tttgatctga tgggttcgct ggcggccttt tcctgcctgg ttttgatact
      361 tatttggcgt gaaaataagc gttaaggagt tttttggctt tttggcaaag caaatcaaag
      421 gcttttccta tttttgtttt tttattgctt caaccgaccg tactatgaaa tcattgctcg
      481 tttttctcct cttaactgct gccggattta atgcctactc ttcggctaaa atctgggtgt
      541 cgccggaggg ctccgactcc aaccccggca cggaacagca agccatggcc agccttcagg
      601 gggctttgcg caaggccagg gaaatgagaa ggctacaaga tccggccgtc gaaaaaggaa
      661 tagaaatcat tttgaaagac ggttactatg ctttgcccga aaccttattg attcgtcccg
      721 aagactccgg cagccgggac agacctacca ccataaaagc agaacattac ggcaaggcaa
      781 ttatcggcgg aggtctcaaa attgaaggct gggaaccctt gaagaagcgg gtaaaaggga
      841 tgaacaagag catcggttca aaagtatggg tggccaaagt ccccacctgc ggagggcgca
      901 ggatcgattt ccggcagttt tacgtaaacg gtagaaaagc cggacctgcc gcccattccg
      961 acacgtcctg tatgcagcgt attctggccg tggattatga aaaacaggaa atacacattc
     1021 ccgctcccga acaaagaaac ttcgtcacca ccgacgggat ggagtttttt attcaccaga
     1081 tgtgggaagt ggccatcctg cgcatcaaag atttccggat caaaggaaat gaggcaatcc
     1141 tgagtttcca tgctcccgaa agccggctgg aatttgaaca tccctggcca ccgcctgtgg
     1201 tctcggaaga gggcaattcc ccctttattc tgagccgggc catcgaattt ctggataaac
     1261 ccggggaatg gtaccacgac cgagaacaag ccttgttgta ttatctgcct ctaaatggtc
     1321 aggatatgca gcaggcagaa tgcatcgtac cggtactgga aacgcttgtc agtgtacaag
     1381 gatcattgga caggccggca caatacatcc ggttcgaggg gctgagtttt gcccacagca
     1441 cctggctgcg tccttcaaaa cagggttatg tgcctttgca ggccggcatg tttttgctcg
     1501 atgcctataa attgcaacaa ccgggaactc acgataaagc caccctcgaa aaccaggcct
     1561 ggataggccg gcaagccgct gccgttgagt tgtacgccgt aaacaatacg gtcttcgaag
     1621 cctgccgttt tgaacacctg ggtgcttcgg ggctggatta catagaagct tcggccgacg
     1681 accgcatcga aggctgcgtt tttcaggata ttgccggcag cgccatagtg atgggccgtt
     1741 tctcggaacc cggcaccgaa acccacctgc cctaccaacc caaagattac cgcgaagtca
     1801 gtcgcaaagc tctgataaca gacaattaca tcttcaactg cgccaccgaa tactggggtt
     1861 gtcccggcat tctggccggt tacgtgcaag aggtggaaat tcggcacaac gaagtctgcg
     1921 aactgcccta taccggcatc agtgtaggct ggggctggac aagaacagcc aactgtatga
     1981 aaaacaaccg tatagtagcc aatcgggtac atcgtttcgg cagacataat tacagttgcg
     2041 ggggcttgta caccctgtcg gcccaaacag gaacgttgat agccgaaaat tacgtacacg
     2101 atatatatcg tcccgaatac gtgcacgaca aaaatcaggg ctattacatt tatctcgacg
     2161 aagcttcctc gtggatgacc atacgcgaca actggtgcag cctgcccaaa ttcggtcaaa
     2221 atcagcccgg cctgaatcac tgggaaaaca acgggccaca ggttgatgaa agtattaagt
     2281 taaaagcagg cattcgccct ccgtggcaat atataaaatc acagccctga ccccaacagt
     2341 tgaataaaca attccgaaag cataacctga acccttgaaa tgtttggatt tggcaagacc
     2401 atagaaattt tatctccgat cgaaggcagc agcctccctt tgagcgagtg cggcgacgag
     2461 gcttttgcgg aggaaatgct gggcaaaggc ttggcggtac tacctgccaa aggcctggtt
     2521 gtggctccct tccatgccaa agtcgaagtg ttgtttgata cagcccatgc cctgagcctg
     2581 ctcagcgatg aaggcgttga gttgctgata catatcggta tcgacaccgt aaaattgcaa
     2641 ggtaagcact tcaaagccct ggtaaaacaa ggtgaccggg tgagagccgg agaagcattg
     2701 atcgaattcg atcctgccgc catccgtgaa gagggctata gcacggttgt ccctgtggtg
     2761 gtttgcaaca gcgccgatta cgccgccgtt gaaagttttc cgggccggaa gctcggaatg
     2821 ctcgataaac cttttattcg aatccgaaaa aaataacaca tgcgtacagg caaaggactt
     2881 ggaatagtga atggaatggc gagcggcaaa cttgtcgtcg ttcagccccg gacaccctta
     2941 catcttgaag cggagaaaac agccgatccc cggcatgaac tggacatgta tttaaaagcc
     3001 agggaagaag ccctgctaca actccggctt ttacaagaac agagcacagc cacattgggc
     3061 aaagaacaag ccgccatttt cgaagctcag gctatgatgc ttatggacga agacttcgaa
     3121 gatgccgtca gctctctaat tctgaaaaaa aatctaagtg caacacaagc ggtcaaagca
     3181 ggcggtagaa aaatagccgc cctgttcctg ggtatggaag acgaacggat gcgcagtaaa
     3241 tcggccgata ttctcgacct ggtacaacgc ttacattcga tattgagcgg acaaacagag
     3301 attcccgttc cgcccccgga agctttcatt ctcctggccg aagacctgag cccaagtgaa
     3361 ttgatacaat ggcgttcacc gctgttaaag ggaattgtac tgagccgcgg ctcggctcat
     3421 tctcacctgg ccatactggc agccgctatg aaaataccct gccttatctc ggccgatatc
     3481 gaacccgatc aggctcgcgg aaatacccga atcgccctcg atgccaccgc cggatttttt
     3541 tacctcgatc cgggagttga atttatgagc cgtttcaatc agaaaatcag tcgagaagat
     3601 tcaaacggac aaattcccgt cgatctgcgg ttttacagcc cccggggaga agaaatcgag
     3661 gtcctggcca atatcggcag cccggaggaa ctcgaccccg acttgcaaaa aaattgtcag
     3721 ggcataggtt tgtttcgcag cgaattcatt tttatgggac gccgggagct tccctccgaa
     3781 gaagaacagt ttgagctgta tcgccgtttg gctacgggca tgaagggcaa aaaagtggtg
     3841 atcagaaccc tcgatatagg cgccgacaaa gacgctccct gtttaaactt agccaaagaa
     3901 gaaaatcccg ccctgggaaa caggggtata cgactgtgcc tgcaaaggcc ggagcttttc
     3961 aggactcagc tcagagcggt ttgccgggct gcggtttacg gtaaactaat gctgttggtc
     4021 cctatgttga tttccgtcga cgaattgctc cgggtaaaac gcctgttgct cgacattcaa
     4081 caagagctta ggcaagaagg gctcgaaatt cccgacctgc ccctgggtgc catgatagaa
     4141 acacccgcct ccgtgatgtt gtgcaaagaa ctggcagcag aggccgactt cctcagcatc
     4201 ggcaccaacg acctgacgca atacaccctg gccatcgacc gtcaaaaccc ggagcttgcc
     4261 tctttatacc aacaggcaca tccggccctg ctccgtatgc ttcgcgaggt gatccgggcg
     4321 gctaaagctg cgcaaattcc ctgctccatc tgcggaggac tggctcagga cacaaaacac
     4381 acagctatgc tgatagacat gggattcagc gcattctcca tggctccggt gcatattccc
     4441 ggcttcaaaa aattcctgta cgaacactat tcccgctccg aaccttcgct ctaaacccac
     4501 gctatgacaa gttttacaca tacgatcgcc gatccgctgg gcatccatgc caggccggcc
     4561 gccctgttgg ttaaaacagc ttctgccttt cgttcttcga tcatcctgcg ccacggggat
     4621 aaggaaatca gtgcaaaaaa aatgatcgcc ttgatgagcc tgggccttaa acaaggcgat
     4681 aaactggaaa tacgtattga aggagaagac gaagagcaag ccggccaggc tatgaaaaaa
     4741 atcttagaag aacagctatg atgaaatacc tgcaaagact gggcaaatcg ctgatgttgc
     4801 cggtggcttg tctgccggtg gccggtattt tgctgggtat cggctattgg ttggaatctt
     4861 cgttgggcag caatattccg gctactttct tattacaggc cgggggagcc atactcaacc
     4921 acatccccct attgtttgcc gtgggcgtag ccgtgggaat ggccgacgaa agcaacggag
     4981 cctctgccct ggccggactg gtcagttggc tggttataac cactttactc tctccggcca
     5041 ctgtccaaat gttcagcgga caaacggccg atccggcctt cgaaaaaatc gaaaacgttt
     5101 ttacgggtat tctttcgggc ataacagctg ccttttgcta caaccgtttc aaaaacactt
     5161 ccctgcccga tgcccttgct ttttttggag gcaaacgctc tgttccgatc gtggcggccg
     5221 gcctggcctg cttgataagt attcttttgt tttttagctg gcccctgatg tacaacggat
     5281 tgatcctctt cggtaaaagt attttggata ccgggcctgt gggcgccggg ctgtatgctt
     5341 tcttcaaccg cctgctcatt ccgctgggat tacatcatgc gctgaatcag gtcttctggt
     5401 tcgatgtggc aggcatcaac gacctgagca acttttggac cgggcaaggc acactcggaa
     5461 ttacaggtca atacatgacc ggcttcttcc cgataatgat gtttggcctg cctgccgctg
     5521 ccctggctat gtaccacacc gccagacccg aacgtaaaaa agcggtggga ggactgatgc
     5581 tcgcctcctc tctgagtgct ttcctgaccg gcgtaaccga acctctggag ttttctttta
     5641 tgtttctggc tcccggcctg tacgtcctgc acgccttgct gacgggactg accgcaggca
     5701 tcgtagctta tctgcctgta agggcgggtt ttaatttcag tgccggcctg gtggattatc
     5761 tgctgagttt taaatcgcct atggctctga atcccctccg gataatcgga ataggactcc
     5821 tgacagccct ggtctattac ctggtcttcc gttttacaat tcaagccttc ggtctacgca
     5881 caccgggcag ggaagaagaa aaccgggaaa ctccgccgga cgaagaaatt cgaagaactc
     5941 caccgggcaa tgaaagccgg gaggccctgc cgggcggaga taaagacaag gaggatggcc
     6001 gaaagggcaa aaaagctccg gccggaaaag gtaaaaaaac cagggccgga aaaggcaaaa
     6061 taactccggc cctagggagc aagaacagcg ataattaccg ggaaacagcg gctcttctga
     6121 tacagggcct cggcggcaaa gaaaacattg ccgaactgga caactgcatc tcgcggctcc
     6181 gcattgtgat tagacaagac gaaacggtgg acgaaaccat actaaaatcc gctgctattg
     6241 cgggcatagt ccggccgggc aaaaacacca ttcagttgat tgtcggtccc aaagtgcagt
     6301 ttctggccaa agaaatgaaa caactgctta ctaaataaaa gcctatctat atgactatta
     6361 aagacattat tcagtcccgg caaacgcaat tcctcgacga attgttcagc ctgatgcgca
     6421 ttccttcggt gagcggacag caagctcatt tacaggatat gcaagattgt gcacaacgtt
     6481 ggaaagagat tttactctct tcgggagtgg acaaagccga gatttttcaa accaaggggc
     6541 atcccattgt gtacggagaa aaaatcatcg atcccaaggc tcacacggta ctcgtgtacg
     6601 gccattacga cgtgatgccc gaagatccgg tggaactgtg gactacgcca ccctttgagc
     6661 cccacatcga aaacgggctg atcaaaggcc ggggcgccga cgacgacaaa ggccagtcta
     6721 tgatacaggt caaggctttt caactggctc tcgaaaacaa tctgctggct tgcaacgtga
     6781 aatttctgtt cgaaggcgaa gaagaaatcg gttccatcca tctggaggaa ttctgccgag
     6841 atcataaaga cttactcaag gccgatgtga tcctcgtttc cgacacgagc atgctgggag
     6901 ccgacactcc ctccattacc actgggctgc ggggcatttc ctattgggag gtggaagtga
     6961 ccggagccgc ccgggatttg cattccggca tcttcggcgg agccgttccc aaccccatac
     7021 aggaactgtc cgcaataatc tcccgcctga tcgataagga gggtaaaatc accataccgg
     7081 gtttttacga caaagtgcag gaggcatcgg ccgaagaaag aaccatgttg gccgccgtgc
     7141 ctcacgacga tgaagcctat cgtaaatcgg tgggagcccc cggtctgaaa ggagaaaaag
     7201 gctattctac cattgaacgc accggcattc gacccacgtt agatgtttgc ggtatttggg
     7261 gaggctatac cggcgaagga gtgaaaacgg tgcttccttc caaggcttac gccaaaatat
     7321 ctacccgcct ggtgccggat caggattatc aggaaatagg ccggctcttc gaagactaca
     7381 tcaggcgact ggctccgccc gaagtcaagg tggaggttcg ctttaagcac gggggcaatc
     7441 cctacgtttg ttctttggac ctgccggcct ataaagcggc tgaagctgcc tatcacaagg
     7501 tgtttggcaa acgtccgctg gccataaggc gaggaggcag catacctgtc attgccactt
     7561 tcgaaaaagt gctcggaatc aaaagcatcc tgatgggctt cggcctggaa tccaacgcta
     7621 tccactcccc caacgaaaat tttcccttga atctttggct gcaaagcatc gaagttgttg
     7681 tggaatttta ccgtctttat aagtaattgc atcatgaata gtcgctcctt ggggtttcgg
     7741 tccgggtccg ggtttagggc tgagtttaag tttttgtttc tggttctgcc cctcgctctg
     7801 gtgttttctg cctgttccgg accctgtttc cgggtcagcg aaaacggagt atttatccgc
     7861 cccgaccagg ggaaaaccgc cacccgcagt ctctatttac aagtggtcga cgacgatata
     7921 atcagggtta tagccagtcc caaccgaaaa atcagcctga aagccggact gatgtgcaac
     7981 tggcaagaca aagcattcag tccatcgggt cgatggcaag tgtttcgtag cggagacagt
     8041 gtagttctgc aaacggctgc cctgaaagtc accgcgttgt tgagtagcgg agaacttagt
     8101 ttcagcgact taaacggttc ggtgtttttg cgggaacagt tcggcggggg taaaagcttt
     8161 agtccgttcg aatacgaagg atgccgggct tattctttca ggcaggtatt tgaatcgcct
     8221 ccggatgaag cttttttcgg cctgggtcag catcagtcgg acgaattcaa ttacaaaaac
     8281 aagcacgaag ccctgtatca gtacaacacc aaggtctcgg tgccttttgt ggtttcgagc
     8341 aacaactacg gcctgctctg ggacaacaat tcttacagca aatggggcga tccccgggat
     8401 tttaccgact tagatcaatt caggctgttc gacaagtacg gtaaagaagg cgggctgact
     8461 gccttttatt cgaatcccgg ccgaaaaaaa gaattggaac gtgtcgaaaa agccatcgat
     8521 taccaaaatt tgaactctat aaaaaatttt cctcaggctt tcccgctgca aaatgcccgg
     8581 gtaagctggg aaggatccat cgaaacagat accgcgggct tgtttcgttt tgcgctctat
     8641 tacgcgggct acgtcaaact gtacctcgac gatacgctgg tggtgccgga gcgctggcgc
     8701 accgcctgga accccaacac ctgcaagttt tcgtatcccc tgcaggccgg ccaaaagcac
     8761 cggatcaggc tcgaatggca acccgacgga ggcagttcct acatagggct caaagccctg
     8821 agtcccctgt cgtccgggga agaaggaaaa atttctttct ggtcggaaat gggcaagcaa
     8881 atggattatt acgtggtcag gggtaggaac gccgacgaag tggtgagtgg ctaccgcagg
     8941 cttaccggca aggcacaggt catgcccaaa tgggcgatgg gcttttggca gagccgggag
     9001 cgctacaaaa cccaggacga acttttgtcg gtcctccgcg aattcaggcg ccggcagatc
     9061 cccatcgaca acatcgtgca ggattggtcg tactgggaag aaaatgcctg gggcagtcat
     9121 gaattcgatg cgcagcgctt tcccgatccg gaacaaatgg tcggagatgt ccacgacctg
     9181 aatgcccgga taatgatttc ggtctggccc aagttttaca tcaacaccgg gcattacaaa
     9241 gagtttgaac aaaaaggctg gatttatccc cgggctgtca aggacagtat ccgcgactgg
     9301 ataggccggg gctacatcgg ttcattctac gatgctttca gtccgggtgc ccggaagctc
     9361 ttctgggaac aaatgcagga acatctctac gtcaaaggct tcgatgcctg gtggatggat
     9421 gcttcggagc cggacatcct gtctaatgcc gatatggatt accgcaaaca actgatgcat
     9481 cccaccgctc tgggcccatc ggctctacat ttcaatgctt acggattgat gaatgccaaa
     9541 ggcatttacg aaggtcaacg ctcggtaaac gacagcaaca gggtttttat cctgacccgc
     9601 tcgggctacg ccggttcaca acgttttgca gcagccgtct ggagcggcga catcggcaca
     9661 cgctgggaag acatgaaggc tcagatcagc gccggacaaa atttttccat ttcgggcaat
     9721 ccctattgga ctatggataa cggcggtttc tgtgtggaaa aacgttacga aagagcccgc
     9781 gagggatcgg aagacctgga agaatggcgg gaactcaatg cccgttggca ccagtttggg
     9841 gcttttgtgc ctttgtttcg cagccacggc caatatccct acagggaaat ctggaacatt
     9901 gctcccgaag ggcatccggc ttaccaaacc atgaaatatt actcggaact gcgttaccgc
     9961 ctgctacctt atatatatac gatggccggc cgatgttact tagacgatta caccatcatg
    10021 cgtcccttga tgatggattt cgccaacgac gaccgggtaa tgaatatcgg tgaccaatat
    10081 atgttcggat ctcacttgat ggtctgcccg gtgtacgaat ataaagcccg caggcgactg
    10141 gtttatttcc cccaaaacgc cggctggtac gatctttaca gcggcaagta ttatccggga
    10201 ggacaggaaa gtccggtgga agccccttac gagcgcatgc cggtttttgt gcctgcagga
    10261 agcatacttc ccacgggcaa gcccattcaa cacagtgggg aaaaacaaga aacactgcgt
    10321 ttttatgtat tcagcggaca agacggcaag gcctgcttgt acgaagacga cgcaaccagc
    10381 tacgattacg aaaagggtga ctatgccagg ataaccttcc attacaacga tgcccaaaaa
    10441 agtttgtcga tttcggccat cgagggttcc tatcgtcccg agccgactca gaggcggttt
    10501 gaatttgttt tggtcagtcc ccaatgtcct gccggcctgg ataccgaagt cc
//
LOCUS       DAOSTD010000065        10543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_6149_length_10543_cov_13.437834, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000065 DAOSTD010000000
VERSION     DAOSTD010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10543
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6149_length_10543_cov_13.437834"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="PLI37_05455"
     CDS             complement(<1..1521)
                     /locus_tag="PLI37_05455"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ58487.1"
                     /translation="MKRKFSHQILLALGLALHTVLSSCSDEWLEVFPKDMIVYEDFWK
                     TKAQLESAVSGCYMACLTESESYSVAGQMLLWGELRGDNITAGPSVSDDESKIMSFNI
                     LPSNAICNWKRFYTVINNCNVVLENARFVMDSDKSLSIRDVEAYQAEALALRSLMYFY
                     LVRTFKEVPLVLEASTNSEQNFYTPKTTEDTVLTQIVGDLEKAYTMAMSTHDRLEYDK
                     GRFTKTSIAALLADVYLWKEDYENCIRCCDIVLSNKELVMTKDRTGTGMIWRQNIFVY
                     GNSNESILEFQYGNSNQGIVNNALFRSFGSSSRNPHFNVPLDITSWDLNSIFGKKDFR
                     AKMSYTKESGASSGKSSKFQESESSGANWIVYRLPEIILMKAEALVQLNFETNKEQAL
                     QLVNTVYMRAQNTPAVDDSLQMAAYDEKMLMDDLILAERQRELMFEGKRWFDIMRAAR
                     REKTMRVFSKYILPNVEGSYYELASAKLTKEWARYFPVPLSEMESNSLLTQNPFYET"
     gene            complement(1533..4808)
                     /locus_tag="PLI37_05460"
     CDS             complement(1533..4808)
                     /locus_tag="PLI37_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOQ58488.1"
                     /translation="MKRIKITVVCLVCLSWTLTAYAQQIIVRGLITSEDREPLISVTI
                     AEIDRNNRIVNGTVSNYDGEYVLQVRDKANRLRFNYVGFKAQDQEIGERTLINVTMQE
                     ESLELAEAQVTGKAITNTGTMNIPTREISFAMQKVDAKEFEGIQVTSVEDALQGRVSG
                     LDIVSNSGAPGSGMSMRIRGTTSLRSDGSQPLIVVNGVPFETTVASDFDFATAREEQY
                     AELLTVNPDDILEIVVLKDAASTAIWGAQGANGVLQITTKKGARGRPKVQYTYRLTSA
                     FMPDPIPLLNGDEYTMLMKEEYLNPTLNTSQGNIPEYGYVQDGSAFFWNYNNNTDWVK
                     EITRTAWKHDNNLSISGGGERGDYRISFGYLDEEGIVYGQGLRRFTNRTDLGYVLSDR
                     LIFRTELAYTFSDNDRNYDGDILNKALRKMPNVPVYTRDEKGNYTDVYFNIDRGSSNL
                     HADQRDMVNPVGLVNLASYNSKSYRIIPTFRLQYDILDPGKGLLRFKSSVSMDVNSIH
                     TTRFFPKELSGANFDDTSINYAFDGDEESVNIQLDNNLTWQPRLRKGHAFMIYGAVQT
                     SINKGSSQSLGSYWAPSSYIQNVSEGFTSETKSYSSESHGYGMFVSSHYAYLERYIVD
                     FVVRRDVSSRFGKNNRVGYFPGISLKWIASDENFLQDVSWINMLAFRPSWGISGNTPK
                     SSYLHYSKYSTYPHSYIDMPAVYPSGLQISNLRWEKTTQLNMGIDFAFIKNRLVGDIN
                     FYHKRTTDLLFQNLPIPASSGFSTQSYENTGVMDNDGWELNMRAPSIVKSGDFSVDIS
                     LNLSNYYNTIMELSDKIELSKGEMINNGEYLRNIIIGNPLGSFYGYRFKGVYQYSEYI
                     EGVQENAPVARDAENKVIYDRFGVPKPMWFNYGGSNAYQFQGGDAIYEDINHDGSIDE
                     LDVVYLGNANPKINGGFSFLFRYKRFSVNAFFHFRYGNQIVNESRMRIENMYSNDNQS
                     IAVKWRWRKEGDVTNMPRALYNTGYNWLGSDRYVEDGSFLRFKYLTLNYSIAPNLIKR
                     FGLTDLRFYLTLNNLALWTNYSGVDPEVGYAATASDPFRIGYDTSKTPRSKDGTFGVT
                     IGF"
     gene            complement(4818..7385)
                     /locus_tag="PLI37_05465"
     CDS             complement(4818..7385)
                     /locus_tag="PLI37_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOQ58489.1"
                     /translation="MTYSTTHGKTRWRLLLSLLVITGLSCNNSYEYLERSPDWIGDNI
                     YSTLQQDGRFDTYLELLSRMDSSYIEVLRRTGSRTLFVPTDSAFAAFFADNPYGFAGP
                     ADMSTKLIKNYLSFYTLENAYVTSVLGSSQGPETGNCLRRTTTMVPDDTIPFRTDLPD
                     NPQFARFKESGMYLKTPGNWTMVHFTQPYFDKKNINNEDFDRLYPGAQRRNGDIHVFG
                     AKIIEGNIVNLNGYIHVLDKVVMPPLSMWEYMTANENLSEFCRLLERFCEPVYDPAAT
                     EKLRERQPESTDSVFYISFFDTADRTIVDDKGNEYTVESLLFSPAANETQNQAIVGQP
                     PSDMPAIFAPDNQSMQHYIENSFLSEYPLWDSVPDDLAAKFVRTHMKRSFLNALPTRI
                     DDIVDDVKGDHMGYTLENVVDAQICRNGLVYTLNDVVDPRDFKTVVAPLLKNPDTRIF
                     NWIINESSNNTALKFNYYLTSLENNFTLFIPLDSSFSAYPDPVNQTSATLEKKKMRFF
                     FDEDKKRVNYISLNNAGDSIAAPTYNADVVRNRLKDIVDHHIVVGERSPGQKYLQTKG
                     GAYIKVDESAQGLRFQGAGDLEKGSYVNVIAKYDYNNGIHNGTVFLIDKTLEHTLRSF
                     YSLAQSNITADSNISEFFKLLQDFDDPDATFFADREPATDKTLFYRNVSAAKQTEYVR
                     PIFYKKDGIDYFVPFLGLYNYTVFVPVNEALNRACTEDRLFMNKDQIASLGNDSAMIA
                     EIKRTIAFIKNHFVDGAYLTEQFYMDEWAEDDGLPRTTTTSAKNQKNRQFYNLYITQN
                     GSHLSIKKNAAGPVLGTTLSGSENIMCRQYSFAGSNIATNSRIIVHAIDGVLSPNNE"
     gene            complement(7552..10512)
                     /locus_tag="PLI37_05470"
     CDS             complement(7552..10512)
                     /locus_tag="PLI37_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HOQ58490.1"
                     /translation="MKKLYLFMGGLLLAASMNLSQAQKPMTEVKIGDLIYISHTSTGR
                     VWSGINESSNIQIRDPKIAFTDTAQIWEIVPAQVKGTDSTLNDADFYYLQEKTGLQRF
                     AASDGGWNSRVNSAVGVIADTTRYYWTFRRSATLENVFHIANGNYFSIDSATWTVKSV
                     SRYMGTDGTGNGSSLYTDKASSNAGAPYYIYDARTYAKFVVAKAISDAEKLIQLLNAN
                     DLSAYGDTLQTALDAVKLIDTTKTYDQYTQADQGKLDEMQAVGAIQAIANVKTWLNAV
                     TALQNKITEAEGKAALDPKYPAGIADSLNMAITDARAYGDTVTYRGSTVAEIQAEQTV
                     LQGFINRLADKLKMVDKLIAKIDEAKAVDQGAAPYPEQSKTDFNAAIATAESYLANIT
                     STTATADIEQATATLNLQMYALLISVATEENPIEVTDLISDPSFEESSGYTLSAKWKD
                     GSVVASGDRRVNSSIVVTDGNQCWNSWSDNFTSMNVYQDLTNLPNGVYRVTCDAITQE
                     GYITDQHLYATTFETNQSTILTATAFNTTPEAWETLSTQFVVFDGTARIGFASTSGGA
                     QAGWFCIDNFRLYYLGIDPDAVETMFRVLLTQIQTDRMEYAVPLGEDPKLDAALDEAN
                     AFLEGVFTPEQLDAMIAKTNAAWNRFKSSQTAALSLDAYIVEATEMYETADETIYPEE
                     AFSDLIDAINYATELLGAEATLKEDCEQALLDLQEAVRVFKYSAVGSVDNPADFTFVI
                     VNPELNTSNNATVPQGWTAAHENGNGYMGHIQHYRGDGYYYFDAWNGTAGALKYKAYQ
                     KIADLPQGTYVLKMAGRGTNAGNEAGINGVQIYAKAGSRVYSTTVPCEGDGNVSSPDA
                     IPSNFETAIFHEDGVTWYGWNDITVSNIHLYSGDFLEIGVDVGYLSPFTGTWFSACDP
                     RLYYLGPEMGTGSVKAPVKVFAYTLDRQIVVVNSDSPVKVYTITGQEVENRHLVQGIY
                     LVRVDGYTLKVAVK"
BASE COUNT         2373 a         2397 c         2871 g         2902 t
ORIGIN      
        1 ggtttcgtaa aaaggattct gtgtcagtaa agaattggat tccatttccg acaggggaac
       61 ggggaaataa cgagcccatt ctttggtgag cttggccgag gccaattcgt aatacgatcc
      121 ctctacattg ggtagtatat atttactgaa tacacgcatg gttttttcgc ggcgggctgc
      181 tctcataata tcgaaccagc gtttgccttc gaacatcagt tcccgctggc gttcggccag
      241 gatcaaatcg tccatcagca tcttttcgtc gtaggcggcc atttgcagcg aatcgtcgac
      301 ggcaggggtg ttctgggcgc gcatgtacac ggtattgacc agttggagtg cctgctcttt
      361 gttggtttcg aaattgagct gtaccagggc ttcggctttc atcaggatga tttcgggcaa
      421 cctgtaaact atccagttgg caccggacga ttccgattcc tgaaacttgc tgctttttcc
      481 gctggatgct cccgattcct tggtgtaaga catcttggct ctgaaatctt ttttcccgaa
      541 aatgctgttc agatcccagg aggtaatgtc caaaggcacg ttaaaatgag gattgcgcga
      601 cgaagagccg aagctgcgga acagagcgtt gttcactata ccctggttgg aattgccgta
      661 ttgaaattcg aggatgcttt cgttggagtt gccatagaca aaaatgtttt gcctccagat
      721 cattccggtg ccggtgcggt cttttgtcat caccagctct ttgttggata agacgatatc
      781 gcaacaacga atgcaatttt cgtaatcttc cttccacagg tagacgtcgg ccagcaaggc
      841 tgctatggag gttttggtaa atcgtccctt gtcgtattcc agcctgtcgt gggtgctcat
      901 agccatggtg taggcttttt cgaggtctcc gacgatttgt gtcagtaccg tgtcttcggt
      961 ggttttggga gtgtaaaagt tctgctcgct gttggtgctc gcttccaata ccaggggtac
     1021 ttctttgaaa gtgcgtacca ggtagaaata cataagcgaa cgcagggcca atgcttcggc
     1081 ctggtatgct tctacatccc tgatcgaaag gcttttgtcg gaatccatta caaaacgggc
     1141 attttcgagt accacattgc aattgttgat gaccgtgtag aagcgtttcc agttgcagat
     1201 ggcattggaa ggcagaatgt tgaagctcat aatcttggat tcatcgtccg acacgctggg
     1261 gccggccgta atattgtcgc ccctgagttc accccagagc agcatctgac cggctacgct
     1321 ataactttcg gactccgtca ggcaggccat ataacaaccc gaaacggccg attcaagttg
     1381 agctttggtt ttccagaagt cttcatagac gatcatgtct ttgggaaaca cctcaagcca
     1441 ttcgtcgctg cacgaactga gcacggtgtg caaggccagg ccgagggcta acaatatctg
     1501 atgggagaat tttcttttca tcgcgcttaa tgttaaaaac cgatagtgac accaaaggtg
     1561 ccgtctttac ttcgtggagt tttggaagta tcgtatccga ttctgaaggg atcgctggct
     1621 gtggctgcat agcccacttc gggatccact ccggaatagt tggtccaaag ggccaggttg
     1681 ttcagggtca ggtaaaacct aagatcggtc aggccaaatc gctttatcag gttgggagcg
     1741 atcgaataat tcaaagtcag gtatttaaac ctcaggaaag aaccgtcttc cacgtaacgg
     1801 tcggatccca accagttgta gccggtgttg tacaaggccc tgggcatatt ggtgacatcg
     1861 ccttctttgc gccagcgcca ttttacggcg atgctttggt tgtcgttgga gtacatattt
     1921 tcgatccgca ttctcgattc gttcacaatc tggttgccgt aacggaagtg aaagaaagca
     1981 ttcaccgaga aacgtttgta tctgaacaag aagctgaaac cgccattgat cttcgggttg
     2041 gcgttgccca ggtaaaccac gtcgagctca tcgatgctgc cgtcgtggtt gatgtcttcg
     2101 taaatggcgt cgcctccctg aaattggtaa gcgttggatc ccccgtaatt gaaccacatg
     2161 ggtttgggca caccaaaacg gtcgtaaatc actttgtttt cggcatctct ggccacaggg
     2221 gcgttttctt gcacgccttc aatgtattcg ctgtattggt acactccttt aaagcggtag
     2281 ccgtaaaacg agcctaaggg gttgccgatg atgatattgc gtaaatattc tccgttgttg
     2341 atcatctcgc ccttggagag ctcgatttta tcggagagct ccatgatggt attgtagtaa
     2401 ttcgagaggt tcaggctgat atctaccgag aagtcgccgg attttactat ggaaggagct
     2461 cgcatattga gttcccagcc atcgttgtcc atgacgcccg tattctcgta agattgcgtc
     2521 gaaaaaccgg acgaagcggg gatgggcaga ttctggaaca gaagatcggt ggtgcgtttg
     2581 tggtaaaagt tgatatcgcc caccaaacgg tttttaataa aggcaaagtc gatacccata
     2641 ttcagttgag tggtcttttc ccagcgcagg ttgctgattt gcagaccgct ggggtaaact
     2701 gccggcatat cgatatagga gtgagggtag gtggaatatt tactgtaatg caaatacgac
     2761 gacttgggag tattgcccga aataccccag ctaggcctga aagccagcat atttatccag
     2821 gaaacatcct gcaaaaaatt ctcgtccgag gcgatccatt tcagggaaat accgggaaaa
     2881 tagcctaccc ggttgttttt tccgaaacgg gaactcacat cgcggcgcac tacaaagtcc
     2941 acaatataac gctccagata ggcgtagtgc gaggacacaa acatgccgta gccgtgcgat
     3001 tcgctcgaat aagatttggt ttctgaggta aagccttccg acacattctg aatgtacgac
     3061 gaaggtgccc agtaagaacc taagctctgt gagctgccct tgttgatact ggtttgtacg
     3121 gctccgtaga tcataaaggc atggcccttg cgcagcctgg gttgccaggt aaggttgttg
     3181 tcgagttgaa tgttgacgct ttcttcgtcg ccgtcgaaag catagttgat ggaggtatcg
     3241 tcgaagttgg ctcccgaaag ttctttgggg aaaaagcggg tagtatggat gctgttcaca
     3301 tccatcgaaa cggacgattt aaacctgagc aggccctttc cgggatcgag tatgtcgtat
     3361 tgcagcctga aggtgggtat gatacggtag cttttggaat tatagctggc cagattgacc
     3421 agacctacgg gattgaccat atcgcgttga tcggcgtgca aattggacga accccggtcg
     3481 atgttgaaat agacatcggt gtaattgcct ttttcgtccc gggtgtacac aggcacgttg
     3541 ggcattttac gcagggcttt gttcagaatg tcgccgtcgt aatttctgtc gttgtccgag
     3601 aaggtatagg ccaattccgt tcggaagatc agtctgtcgg aaagcacata gcccaggtcg
     3661 gtcctgttgg tgaaacgcct caggccttgt ccgtaaacaa tgccctcttc gtccaggtaa
     3721 ccgaacgaga tgcggtagtc gccccgttcg ccccctccgc tgatagacag gttgttgtcg
     3781 tgtttccagg ctgtgcgggt gatttctttt acccaatcgg tgttgttgtt gtagttccaa
     3841 aagaaagcgg agccgtcctg cacataaccg tattcgggaa tgttcccctg actggtgttc
     3901 agggtggggt tgaggtactc ttctttcata agcatggtgt actcgtctcc gttgagcagg
     3961 ggaatgggat cgggcataaa ggctgaggtc agccggtagg tgtactgaac tttgggtctt
     4021 ccccgggcgc cttttttggt ggtgatttgt aacacgccgt tggctccctg tgctccccag
     4081 atggcggtgg aggcggcgtc tttgagtacc acgatttcga gaatgtcgtc ggggttgacg
     4141 gtgagcaatt cggcgtattg ttcttcgcgg gctgtggcaa aatcaaagtc ggaggctact
     4201 gtggtttcaa aaggcacgcc gttgaccaca atcaagggtt gtgaaccgtc gctgcgcagc
     4261 gaagtggtac cgcggatacg catagacata cccgagccgg gagctcccga gttggacacg
     4321 atgtccagcc cgcttaccct tccctgcagg gcgtcttcga cagaggtcac ctgaatgcct
     4381 tcgaattctt tggcatcgac cttttgcatg gcgaaagaga tttcgcgggt gggtatgttc
     4441 atggtgcccg tattggtgat tgctttgccg gtcacttgag cctctgccaa ttcgagggat
     4501 tcttcctgca tggtcacatt aataagggtt cgctcaccaa tctcctgatc ctgagctttg
     4561 aatccgacgt aattaaagcg cagcctgttg gctttatctc tgacttgcaa cacgtattca
     4621 ccgtcgtaat tgctgaccgt tccgttcaca atacgattgt ttctgtcgat ttctgcgatg
     4681 gtaacggata tcagcggctc ccggtcttcc gaagtaatca gtccccggac aatgatttgc
     4741 tgtgcatagg ccgtcagagt ccacgacagg cataccaagc aaactactgt gatctttatt
     4801 cttttcataa ctgtttatta ttcgttgttg ggggacaaga ctccgtcgat ggcatgaaca
     4861 ataatgcggg agttggtggc aatgttactg ccggcaaagc tgtattgccg gcacatgatg
     4921 ttttcgctgc cgctaagggt ggtgcccaga acggggccgg cagcgttttt cttgatactc
     4981 agatgtgaac cgttttgggt aatgtacaaa ttatagaatt gcctgttttt ctggtttttg
     5041 gccgaagtgg tggtagtgcg gggcagtccg tcgtcttcgg cccattcgtc catataaaat
     5101 tgttcggtaa gataggcacc gtcgacaaaa tgatttttta tgaacgcaat ggttcttttg
     5161 atctcggcaa tcatggccga gtcgttgccc agagaagcaa tctgatcttt gttcataaac
     5221 agccggtctt cggtgcaagc cctgttcaag gcttcgttga cgggcacaaa gaccgtgtag
     5281 ttgtacaagc ccagaaaggg cacaaaataa tcgatgccgt cttttttgta gaaaatgggc
     5341 ctgacgtatt cggtttgttt tgctgccgag acgttccggt agaacagggt cttgtcggtg
     5401 gccggttcgc gatcggcaaa gaaagtggcg tcgggatcgt cgaagtcctg gagtagttta
     5461 aaaaattcgc tgatgttgct gtcggccgta atattgctct gtgccaggga atagaaagat
     5521 ctcagggtat gctccagggt tttgtctatc aaaaagactg tgccgttgtg gatgccgttg
     5581 ttgtagtcgt attttgcaat aacgttgaca tagctgccct tttccagatc gccggcaccc
     5641 tgaaagcgca aaccctgggc gctttcgtcc actttgatat aggcccctcc cttggtttgc
     5701 aggtattttt gtcccgggga gcgttcgcct accactatat ggtggtccac gatgtctttg
     5761 agcctgttcc gaaccacatc ggcgttgtat gtgggggcgg caatggagtc gccggcgttg
     5821 ttgagcgata tgtaattgac acgtttttta tcttcgtcga agaagaagcg cattttcttt
     5881 ttctcgaggg tggcagaggt ttggttcacc ggatcggggt aggccgagaa actggaatcc
     5941 aaaggtataa acaaggtgaa gttgttttcg agggaagtca ggtagtaatt aaacttcaga
     6001 gccgtattgt tgctgctttc gttgatgatc cagttgaata tgcgggtgtc gggatttttg
     6061 agcagaggag ccaccaccgt tttgaagtcg cggggatcga ccacatcgtt cagcgtgtaa
     6121 accagaccgt tgcggcagat ttgagcgtcc actacatttt cgagtgtgta gcccatgtga
     6181 tcgcccttta cgtcgtccac gatatcgtct atccgggtgg gcagggcatt caaaaaggaa
     6241 cgcttcatgt gtgtccttac gaatttggcg gccagatcgt cgggaacact gtcccaaagc
     6301 ggatattcgc tcaggaagct gttttcgatg taatgttgca tggactggtt gtcgggagca
     6361 aaaatggccg gcatgtccga aggcggttgc cccacgatag cctgattttg ggtttcgttg
     6421 gcggccggcg aaaagagcag gctttctacc gtgtattcgt tgcctttgtc gtcgactatg
     6481 gtacggtcgg ccgtatcgaa gaaactgata taaaagaccg agtctgtact ttcgggctgc
     6541 ctttcgcgca gtttttcggt ggccgccgga tcgtagacgg gttcacagaa acgctccagt
     6601 aaacgacaaa attccgagag gttttcgttg gccgtcatat attcccacat gcttaaaggc
     6661 ggcattacca ctttgtccaa cacgtggatg tagccgttta ggttcacgat attgccttcg
     6721 atgattttgg ctccgaaaac atgaatatct ccgtttcggc gttgagcgcc cggatagagg
     6781 cgatcaaagt cctcgttgtt tatgtttttc ttgtcgaaat agggctgggt aaagtgtacc
     6841 atggtccagt tgccgggagt tttgaggtac atgccgcttt ctttaaaacg ggcaaattgc
     6901 ggattgtccg gcaagtcggt tctgaagggg atggtgtcgt ccggaaccat ggtggtggtg
     6961 cgcctcaggc agttgccggt ttccggcccc tgggaactcc ccagcacgga ggtaacgtag
     7021 gcgttttcga gggtgtaaaa actgaggtaa tttttaatga gcttggtcga catatccgcc
     7081 gggccggcaa agccataggg attgtcggca aaaaaggccg caaaggcaga gtcggtgggc
     7141 acaaacagcg tgcgactgcc ggtgcgcctg agtacttcta tataggaact gtccatacgg
     7201 gaaagcagtt cgagataggt atcgaagcgc ccgtcttgtt gtaaggtgct gtaaatgttg
     7261 tctcctatcc agtcgggcga gcgttcgagg tattcgtaac tgttgttaca gctcagtccg
     7321 gtaataacaa gaagagaaag cagtaaacgc caacgcgttt tgccgtgcgt agtgctgtac
     7381 gtcataggaa atggatgtta gatggcatac aaaggaaatc gatctgtgct gtaaaggggt
     7441 gtaaaattca ttcaaaatac acgaaaatac agggcgagaa ccggtaaaaa aaacaggagc
     7501 gggaatattc tcgctcctgt tcggttttat cgctgtttaa tcacttgtta tttatttaac
     7561 agctactttc agagtgtatc catctactct taccaaataa atgccttgaa ccaggtggcg
     7621 gttttcgact tcttgtccgg taatggtgta gactttgaca gggctgtcgg agttaacaac
     7681 tacgatttgt ctgtcgagtg tgtaggcaaa caccttgacg ggtgctttta cggaacctgt
     7741 tcccatttcg gggccgaggt agtacaacct gggatcgcaa gccgagaacc aggtgccggt
     7801 gaaaggactc agatagccca catccacgcc aatctcgagg aagtcgccgg agtagaggtg
     7861 aatgttcgac acggtaatgt cgttccaacc gtaccaggtc actccgtctt cgtggaagat
     7921 ggctgtttcg aaattagatg ggatggcatc gggtgagctc acgttaccgt cgccttcgca
     7981 gggaacggta gtagaatata ctctggatcc ggctttggcg taaatctgca caccgtttat
     8041 accggcctca tttccggcgt tggttcccct gccggccatt ttcagaacgt aggtaccctg
     8101 aggcaaatcg gcgatttttt ggtatgcctt gtatttgagc gcaccggcag taccgttcca
     8161 ggcatcaaag taatagtaac cgtcgccccg gtagtgctga atgtggccca tatagccgtt
     8221 gccgttttcg tgggcagccg tccagccttg aggaaccgtg gcattattgc tcgtattcag
     8281 ttccgggttg acgatgacaa acgtaaagtc tgccggatta tctacactgc cgacggccga
     8341 atacttaaat actctcacag cttcttgcag gtctaacaga gcttgttcgc aatcttcctt
     8401 gagcgtagct tcagcaccga ggagttcggt ggcgtaattg atggcatcga tcaggtctga
     8461 gaaagcttct tcggggtaga tcgtttcgtc ggctgtttcg tacatttcgg tagcttcaac
     8521 tatatatgcg tccaggctca aagctgctgt ctgagaactt ttaaagcgat tccaggcagc
     8581 attggtcttg gcaatcatag cgtcgagttg ttccggggtg aatacacctt cgaggaaggc
     8641 attggcttcg tcgagggctg catccagttt aggatcctca ccaagaggaa ccgcgtattc
     8701 catgcggtcg gtttgaattt gagtgagcag cactctgaac atggtttcaa cggcatccgg
     8761 atcaatacct aaatagtaca ggcggaaatt gtcgatgcag aaccagccgg cctgagctcc
     8821 tcccgaagtg gaagcaaaac cgatacgggc agtgccgtcg aagaccacaa attgggtact
     8881 gagcgtttcc caggcttcgg gagtggtatt gaaagctgtg gctgtcaaaa tggtggattg
     8941 gttggtctcg aaggtagtgg cgtataaatg ttggtcggta atgtagcctt cttgtgtaat
     9001 ggcatcgcag gttaccctgt aaaccccgtt aggcaggttg gtcaggtcct ggtaaacgtt
     9061 catagaggtg aagttgtccg accagctgtt ccagcactga ttgccgtcgg ttaccacaat
     9121 ggagctgttt accctgcggt cgccgctggc caccacactg ccatctttcc atttggcact
     9181 tagggtatag ccactggatt cttcgaagga ggggtcagaa attaaatctg ttacttcgat
     9241 ggggttttct tcggtggcca ccgaaatcag cagtgcgtac atctgcaggt tcaacgtagc
     9301 ggtggcttgt tcaatatcgg ctgtggctgt ggtgcttgta atattggcca gatagctttc
     9361 tgccgttgca atggcggcgt tgaagtcggt cttgctttgt tcgggatagg gggcggcacc
     9421 ttgatctaca gctttggctt cgtcaatttt ggcgatcagt ttatccacca ttttcaattt
     9481 gtcggccaaa cggtttataa atccttgcaa aacagtttgt tctgcctgaa tttcagctac
     9541 tgttgatccc cggtaggtaa cggtgtcgcc ataggctctg gcatcggtaa tggccatatt
     9601 cagcgagtcg gcaatgccgg cgggatactt gggatcgagc gctgcctttc cttcggcttc
     9661 ggtgattttg ttctgcagag ctgtcactgc atttagccag gttttgacgt tggctatggc
     9721 ctgaatggcg ccgacagctt gcatttcatc gagcttgccc tgatcggcct gggtgtactg
     9781 atcgtaagtt ttggtggtat cgatgagttt gacggcatcc aaagctgttt gaagcgtatc
     9841 tccataggcc gaaagatcgt tggcgttgag cagttgaatc aatttttccg catcgcttat
     9901 agctttggca acaacgaact tggcataagt tctggcgtcg taaatgtaat aaggagctcc
     9961 ggcattgctc gaagccttat cggtataaag gctgcttcca ttgccggttc cgtcggtacc
    10021 catgtatctg ctcaccgatt ttacggtcca ggtagcagaa tcgatagaaa agtaattacc
    10081 attggcaatg tgaaatacat tttcgagagt ggcggaccgt ctaaaagtcc agtaatatct
    10141 cgtcgtatcg gctataacgc ctacagcgga attgaccctc gaattccagc ctccgtcgga
    10201 ggcagcaaag cgttgcagac cggttttttc ctgcagatag taaaaatcgg catcgttcag
    10261 cgtagagtcg gtgcctttta cctgagcagg gacaatttcc cagatttgag cggtatccgt
    10321 aaaggctatt ttcggatcgc gtatctgaat gttggaactt tcgttgattc cgctccagac
    10381 cctgcccgta ctggtgtgtg aaatgtaaat cagatcaccg attttaactt cggtcatggg
    10441 tttttgagcc tggctcagat tcatactcgc ggccaataac aggccaccca taaataagta
    10501 gagttttttc atatgcgtac gtgtttaaag aatgttggtt aga
//
LOCUS       DAOSTD010000066        10483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_6192_length_10483_cov_15.047852, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000066 DAOSTD010000000
VERSION     DAOSTD010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10483
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6192_length_10483_cov_15.047852"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /gene="lysA"
                     /locus_tag="PLI37_05475"
     CDS             complement(<1..1103)
                     /gene="lysA"
                     /locus_tag="PLI37_05475"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOQ58491.1"
                     /translation="MKGCFPLSSFNSLRTPFYYYDLDVLRSTLDLLRKEADRNEMEVH
                     YAVKANANPRILDIIRAYGFGADCVSGGELQAAFQAGFPASGLVFAGVGKADWEIDLG
                     LEGNIFCFNVESVPELEVIDQRAGAMGKKAGVALRINPNVQADTHHYITTGVNDSKFG
                     ISLQSLDEVLDICLKLPHIELIGLHFHIGSQITEMDNFAGLCARVNEIQNLLDRRRLF
                     VQHINLGGGLGVDYHHPDQVPVPDFADYMQTLRRMLVLRPGQSLHIEPGRSVVAQCGS
                     LISKVLYIKKGLQNEFVILDAGMTELIRPALYQAYHKIENISSEEPEQKYDVVGPICE
                     SSDCFGKQVVLNKTRRGDLIAIRSTGAYGETMAS"
     gene            complement(1112..2425)
                     /locus_tag="PLI37_05480"
     CDS             complement(1112..2425)
                     /locus_tag="PLI37_05480"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOQ58492.1"
                     /translation="MKVMKFGGTSVGSAQRMKEVAGIVAKEGKNIIVLSAMSGTTNTL
                     LEISDYLHKRNPDGANEIINMLELKYLREIEDLFESKEYKIQTLEVIKSHFDHIRSFT
                     KKVFTLFEEKVIVAQGELLSSVMFTNYLKERGLNAVLLPALEFMRINKNAEPDQNYLR
                     EHLNALLQQHQDADVYITQGFICLNAYGEIDNLQRGGSDYTASLIGAAVKAKEIQIWT
                     DIDGMHNNDPRIVDNTSPVSFLNFDEAAELAYFGAKILHPTCILPAKLEKIPVRLLNT
                     TKPDAKGTTISNKSEKHKIKAVAAKDNITAIRIKSGRMLLAYGFLRRVFEIFESYRTP
                     VDMVCTSEVGISMTIDNNKHLKEILDELKKIGTVSVDRDMVIICVVGDLTWDNIGFEA
                     KVVEALKDIPVRMISYGGSNYNISLLVKAEDKKRSLIALSEQLFK"
     gene            complement(2430..3122)
                     /locus_tag="PLI37_05485"
     CDS             complement(2430..3122)
                     /locus_tag="PLI37_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ58493.1"
                     /translation="MEDTILIHFDKVDICLEDFIVLKEVSFDIRPGEFIYLIGRVGSG
                     KSSLLKSLYAEIPVTEGKARIYDYNLHKIKRKQVLALRRGLGIVFQDFKLMTDRTVWN
                     NLNFVLRATGWKDKNEIEERIDKVLTQVGMQNKGYKMPHELSGGEQQRIVIARALLNN
                     PRIILADEPTGNLDAETGDAIVQLLHDIKDRGTTVLMTTHNLSFLDKFPGRVFKCDHF
                     RLSEETNTIKME"
     gene            complement(3124..3720)
                     /gene="hisIE"
                     /locus_tag="PLI37_05490"
     CDS             complement(3124..3720)
                     /gene="hisIE"
                     /locus_tag="PLI37_05490"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOQ58494.1"
                     /translation="MKPDFAKMGGLVPAIVQDHVTGKVLMLGFMNQEALDKTLETGKV
                     TFFSRSKGRLWTKGEESGHFLHLVSVKEDCDKDTLLIQANPEGAVCHLGWDTCFNEKN
                     SSGDLDFLLYLQDFIDRRYREMPEGSYTTSLFKEGVKRMAQKVGEEAVETLIEAVDGN
                     DERFLYEASDMLYHLMVLLTSKGYRLEDLARELKKRHK"
     gene            complement(3723..4481)
                     /gene="hisF"
                     /locus_tag="PLI37_05495"
     CDS             complement(3723..4481)
                     /gene="hisF"
                     /locus_tag="PLI37_05495"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOQ58495.1"
                     /translation="MLAKRIIPCLDIKDGQTVKGVHFVDFRRAGDPVELGEAYSKQGA
                     DELVFLDITASYEGRKTFTDLVSKVAARISIPFTVGGGIHELADIDRLLEAGADKVSI
                     NTAALLKPQLIDEIARNFGSQICVVAIDAKLEEDGKWRCYINGGHRPVERELLDWALE
                     AQDRGAGEILFTSMSHDGVKNGYPNEALAMLADRLSIPLIASGGAGTMEHFKEAFVLG
                     KADAALAAGVFHFGEIKIPELKAYLKKEGITVRE"
     gene            complement(4475..5194)
                     /gene="hisA"
                     /locus_tag="PLI37_05500"
     CDS             complement(4475..5194)
                     /gene="hisA"
                     /locus_tag="PLI37_05500"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOQ58496.1"
                     /translation="MEIVPAIDLIDGKCVRLSQGDYGRRKVYSDSPLEVAKSFEAAGI
                     RRLHLVDLDGAKAQHIVHHRILEQITSHTSLIVDFGGGLKTDEDLRIAFESGAQMVTG
                     GSIAVKSPEAFENWINTYGPERIILGADVSEGKIAVSGWTERSELDLWSFLENYVQKG
                     INKVICTDISRDGMLRGPALELYTQILAKYPELHLIASGGVSSLKDLDDLKAAGLPAA
                     ILGKAIYEGRIQLKDLSKYLC"
     gene            5484..6188
                     /locus_tag="PLI37_05505"
     CDS             5484..6188
                     /locus_tag="PLI37_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOQ58497.1"
                     /translation="MVKKQKSIEQGELEDLLEVCQFLNPKQKEEFICNCEIHRLKKNQ
                     MLYAEGDKPEHIMCLLRGKIKITKKGVGGRNHIVRMVRPGGYFAYRASLAGTMHSTSA
                     TAFEASDVGVFPSGLFIKLLRQNGELAFYFIKALAVDLGFSDSRQVNLTQKHIRGRLA
                     ESLLFMRNHYGLDEDGATINIYLSREDLANLSNMTTSNAIRTLSMFVSEKVITLDGRR
                     IKIIDEDKLERISRLG"
     gene            complement(6270..7148)
                     /gene="miaA"
                     /locus_tag="PLI37_05510"
     CDS             complement(6270..7148)
                     /gene="miaA"
                     /locus_tag="PLI37_05510"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOQ58498.1"
                     /translation="MQLPNECNLLVILGPTAGGKTKFAVEMAGRLNAEIISADSRQVY
                     RGMDIGTGKDLSEYTLPGGQKIPYHLIDICPAGSRYNLYEYQRDFTRVWRDIRSRNKL
                     PILCGGTGLYIEAVLKGYTLPAVPENPALRASLEGKSLAELSDILAGYKNLHNTSDTD
                     TPKRALRAIEIAEYLATHPEIKSDLPPLQPYIIGLEVEREERRRRISQRLQARLDSGM
                     VEEVRNLLQSGVAADDLLYYGLEYKYLTLYVLGELSYDEMYRQLETAIHQFAKRQMTW
                     FRGMERRGLKIHWIKA"
     gene            complement(7504..8610)
                     /locus_tag="PLI37_05515"
     CDS             complement(7504..8610)
                     /locus_tag="PLI37_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOQ58499.1"
                     /translation="MSTNKNRWFLIAAVAFALVITMLAVTFIMPEQTNRGKKAQAVCF
                     VSFPDFFNFDIPNPWPGYEEAVDYFLTQVKAENPHFVLVAGDLVNGHWWDSPKCVEHM
                     GSIYYSAWVRRMNEYGLKYYTALGDHDLGDDPWPEHKIKLIPCFERVYAEHLRMPQNG
                     PENKKGLAYYVREGDLLIVTVETFEVIDDSMRITVCGEQLEWFEQVLEDHSDAKFKIV
                     QGHVGLWGEINRRSSSALMLEGGKESEFYQLMKKHEVDAYLGGEFHDVTVLESDGIWQ
                     IIHGSSWGREIVNTQDYLVARVKGNELKLTMKRIYMDAQGEYMWNLNKDRGPRETVRI
                     NEKTLKNGPETTGTLTIRRENNQREFLDRTGYFE"
     gene            complement(8685..>10483)
                     /locus_tag="PLI37_05520"
     CDS             complement(8685..>10483)
                     /locus_tag="PLI37_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOQ58500.1"
                     /translation="YCRHQAFVSEVGKASSFPERSVVLTDSWVKRREDLNIEYLKSLD
                     PDRLLHNFRVNAGLPSTALPLEGWESPTTGLRGHFTGHYLSAAALVVDKYGDELLAER
                     LNYMTEELYKCWQSFGNGYLSAFPESDFDVLETRFGGVWAPYYTYQKIMQGLLDVYTR
                     TGNAKAYEMLLGMADYVEKRMSRLSDDTIEKMLYSVGANPSNEAGAMNEVLYNLYKVS
                     KDPGHLALAALFDRDWFVVPLSKNEDILSGLHANTHIALVNGYAQRYAVTKEKKYYDA
                     VVYFWDRLMESHVYANGTSSGPRPNVTTYTSLLAEHWGVPGQLSNTMTGEIAETCVTH
                     NTQKLSSTLFTWKLDPVYADAYMNTFYNAVMALQSAKTGRCVYHLPLGSPRQKAYLRE
                     NDFRCCNGTSIEAFSALNTGIYYRNDTALWVNLYVPSKVHWDEKKLVLEQTGHFPADT
                     CVEFSLTTAKKIDLTLKLFIPSWARKAEVYVNGEKQEAKATPNSYISLHRSWKNKDKI
                     QLVFQYDFYLKTMPDDNNVFAIFYGPMLLAFDSNEELIMKGNHESILQQLSKDKESNS
                     FRLNNNGKTYRLIPLFDIEDQSYGVYATIRNY"
BASE COUNT         2548 a         2695 c         2374 g         2866 t
ORIGIN      
        1 gaagccatgg tttctccata ggctcccgta gaacgtatgg ctatcaggtc tccccgccgg
       61 gttttgttga gtactacctg ttttccaaaa caatcggacg attcacaaat ggggccgacc
      121 acatcgtatt tttgctcagg ttcttccgaa ctgatattct cgatcttgtg gtaggcctga
      181 tacagagccg gccgtataag ttcggtcatg ccggcatcca aaataacaaa ttcgttttgc
      241 aagcctttct ttatgtacaa aactttggaa atcaaactgc cgcattgagc caccaccgaa
      301 cgtcccggct caatatgcaa actttgtccg ggccttaata ccaacatcct gcgtaaggtc
      361 tgcatataat cggcgaagtc gggaacaggc acttgatccg gatgatgata atccacgccc
      421 agtcctcctc ccagattgat gtgttgaaca aaaagccggc gccggtccag aaggttctga
      481 atttcgttca ccctcgcaca aagacctgcg aaattgtcca tctcggtaat ttgcgaaccg
      541 atatgaaaat gcaagcctat cagttcaata tgcggcagtt tcaaacaaat atcgagtacc
      601 tcgtcgagac tttgcaacga aataccgaat ttactgtcgt tcacgccggt ggtgatgtaa
      661 tgatgcgtat cggcctgtac gttggggttg attcttaaag ccactccggc ctttttgccc
      721 atggccccgg cccgctggtc tattacttcc agttcgggaa ccgattctac gttgaaacaa
      781 aaaatattcc cctcaagtcc caggtctatt tcccaatcgg ctttacccac tcccgcaaac
      841 accagcccgg aagccgggaa accggcctga aaagcagcct gcaactcacc tccgctcaca
      901 caatcggcgc caaagccgta agcgcggata atatcgagaa tacgtggatt ggcattggct
      961 tttacggcat aatgcacctc catctcgttt ctgtccgcct ctttacgaag caaatcgagc
     1021 gtggaacgca gcacatccag gtcgtaataa taaaaaggag ttcgaaggct gttgaaactg
     1081 cttaagggga aacatccttt cataatatat tttatttaaa cagttgttcg ctcagggcaa
     1141 tcaaactgcg ttttttgtct tcggccttta ccaataggga aatattgtaa ttgcttcctc
     1201 cgtaagaaat catccgaacc ggaatatcct tgagggcctc caccactttg gcttcgaaac
     1261 cgatgttgtc ccaggtgagg tcgcccacca cacaaataat gaccatgtcg cggtcgacgc
     1321 tgacggttcc tatttttttc aactcatcca aaatctcttt caggtgctta ttgttgtcga
     1381 tggtcatcga aatacccacc tccgaggtgc aaaccatatc tacaggagtg cggtaacttt
     1441 cgaaaatttc gaacaccctg cgcaggaaac cgtaagccaa aagcattcgg cccgacttta
     1501 tacgtatggc ggtaatatta tctttggcgg ctaccgcttt gattttatgc ttttcgctct
     1561 tattcgaaat ggttgtcccc ttagcatcgg gtttcgttgt attcagtaaa cgcacgggaa
     1621 tcttttcgag tttggcggga agtatgcagg tggggtgcaa aattttggct ccgaaatagg
     1681 ccaactcggc cgcttcgtcg aaattcagaa acgacaccgg cgaagtattg tccacgattc
     1741 tggggtcgtt gttgtgcata ccgtcgatat cggtccagat ctgaatttct ttggctttta
     1801 cggctgctcc aatcaaagaa gcggtataat cgctgccgcc cctttgcaaa ttatctattt
     1861 cgccgtaggc attcaggcat ataaaaccct gggttatata tacatcggca tcctgatgtt
     1921 gttgcaacaa agcgttcaaa tgttcacgca ggtaattttg gtcgggttcg gcgtttttat
     1981 tgattctcat aaattcgaga gccggcaaaa gaacagcgtt caagcctctc tcttttaaat
     2041 aattggtgaa catcacgctg gataaaagtt ccccctgcgc cactatcacc ttttcttcga
     2101 acagggtaaa tacttttttg gtaaaactgc gaatatgatc gaaatgcgat ttaatgactt
     2161 cgagggtttg aattttgtat tccttgcttt cgaataagtc ttctatttcg cgtaaatact
     2221 ttaattccaa catattgata atctcgttgg caccatcggg attgcgttta tgcaaataat
     2281 ccgaaatttc gagcagggta ttggtggtgc ccgacatggc cgacaacact attatgtttt
     2341 tgccttcctt ggcaacaatt ccggctacct ctttcattcg ctgcgcagaa cccacagagg
     2401 ttccgccaaa tttcatcact ttcattttat tattccattt ttattgtatt agtttcttcg
     2461 ctcaggcgga aatgatcgca tttgaatacc ctgccgggaa atttatccag aaaactcaag
     2521 ttatgagttg tcatcaaaac agtagtacct ctatccttaa tatcgtggag caattgcaca
     2581 atggcatcgc ctgtttccgc gtccaggttg ccggtgggtt catcggccag tatgatacgg
     2641 ggattattga gcagggccct tgcaattact attcgctgct gttcgccgcc cgacagttcg
     2701 tgaggcatct tgtaaccttt gttttgcatg cccacctgag taagcacttt atcgatgcgt
     2761 tcttctatct cgtttttgtc tttccagccg gttgctctta aaacaaaatt cagattgttc
     2821 cagaccgtcc ggtctgtcat taatttaaaa tcctggaaaa caattcccaa accacgacgc
     2881 aaggccaaca cctgcttacg tttaattttg tgcagattgt agtcgtaaat gcgggctttt
     2941 ccttcggtta cgggaatctc ggcatagagg cttttgagca ggctgctctt tcccgatccc
     3001 acccggccga tgaggtaaat aaactcaccg ggacggatat caaaactcac ctccttgagt
     3061 acgataaaat cttccaggca gatatcgact ttgtcgaaat ggatcagaat agtgtcttcc
     3121 attttattta tgtctttttt tcagttcacg ggccaggtct tccaatcgat agcctttgga
     3181 ggtcaacaac accatcaggt ggtagagcat atcggaggct tcgtacaaaa agcgctcgtc
     3241 gttgccgtcc accgcttcga tcagggtttc gaccgcctcc tctcccacct tttgagccat
     3301 gcgtttcact ccttccttaa acagcgaagt ggtataagaa ccctcgggca tttcgcggta
     3361 acgacgatcg ataaaatctt gcaaataaag caaaaaatcc agatccccgg aactgttttt
     3421 ttcattaaaa caggtatccc aacccagatg acataccgct ccttcgggat tagcctgaat
     3481 caacagcgta tccttgtcgc aatcctcttt tacagagacc agatgcagaa aatggccgct
     3541 ttcttcgccc ttggtccaaa gtcggccttt gctgcgactg aaaaaagtta ccttgccggt
     3601 ctcgagggtt ttatcgaggg cctcctgatt cataaagccg agcatcaaca ccttaccggt
     3661 gacatgatcc tgtactatgg cgggaaccaa gccgcccatt ttagcaaaat cgggtttcat
     3721 cgttattctc ttactgttat gccttctttt tttaaataag ctttcagctc gggaattttt
     3781 atttccccga aatgaaagac gccggctgcc aatgcagcat cggctttacc caaaacaaat
     3841 gcctccttga aatgttccat cgttccggct cctccggaag ctatcagagg gatactcagg
     3901 cgatcggcca gcatagccaa agcctcgttg gggtaaccgt tttttactcc gtcgtggctc
     3961 atactggtaa atagaatttc accggccccg cgatcctgag cttccaaagc ccagtcgagc
     4021 aattcccttt cgaccggccg gtgtccgccg ttgatgtaac aacgccattt gccgtcctct
     4081 tccagtttgg cgtcgatggc caccacacag atctgggagc cgaaattacg ggcaatctcg
     4141 tcgatgagtt gaggttttag caaagcggct gtattgatgg agaccttatc ggctccggcc
     4201 tccagcagac ggtcgatgtc ggccagctcg tgtatgccgc cccccacggt aaagggaata
     4261 ctgatgcggg ctgccacctt gctcaccaga tcggtaaaag tctttcggcc ctcgtaagag
     4321 gccgtaatat cgaggaatac caattcgtcg gcgccttgct tactgtaagc ctctcccagt
     4381 tctacgggat cgccggcccg ccgaaaatcg acaaaatgaa cgcccttgac ggtttggccg
     4441 tctttgatat ccaaacaggg aataatgcgc ttagctaaca tagatactta cttaagtcct
     4501 tcaattgaat acgcccctcg taaatggctt tgcccaaaat cgccgccggc aggcccgcgg
     4561 ctttcaggtc gtcgagatcc ttaagactac tcactcctcc gctggcaatc aggtgaagtt
     4621 ccgggtactt ggccaatatc tgagtataaa gttccagagc cggtccccgg agcatgccgt
     4681 cgcggctgat gtcggtacat atcactttgt tgatgccttt ttggacgtaa ttctccagaa
     4741 agctccacaa atccaattcg gaacgttcgg tccagccgga tacggcaatt ttaccctcgc
     4801 tgacatcagc tcccagtata atgcgctccg gaccgtaggt gttgatccag ttttcgaagg
     4861 cctccggcga cttaaccgca atgcttcctc cggtcaccat ttgagctcca ctttcgaaag
     4921 caatgcgcaa atcctcatcg gttttgagcc ctccgccaaa atcgacaatc agggaagtat
     4981 ggctggtaat ctgctccaaa atccggtgat gcacaatgtg ttgagctttt gccccatcca
     5041 gatcgaccag gtgcaaacgg cgaataccgg ctgcctcaaa gctcttggct acctcgagcg
     5101 gggaatcgct gtaaaccttg cgtcgcccgt aatcaccctg gctgagacgc acacatttac
     5161 catcaataag atctatggcc ggtacaattt ccattgtgtt ataactcaat taaaccatct
     5221 tccacatagg gggtaaacac cggaaaatga atgccggctt tacggcctgc aaaagtaact
     5281 aaaatacgcg gttatcttgt gtgattttag aaaaaagcac aaaatcatcc cgttttttgg
     5341 tcttaatgta ccggtggttt ctgcgtataa atattaattt ttatctttgc ccatccgaaa
     5401 ggtcataaaa ggttagaaaa gatacagact atgctataat cggctcgcaa ggccggttgt
     5461 tttgctttaa aaatctgcct gaaatggtta agaaacaaaa atcgatagag cagggtgaac
     5521 tggaagatct ccttgaagtt tgtcaatttc tgaatcctaa acaaaaggag gaattcatct
     5581 gcaactgcga aatccatcgt cttaaaaaga atcagatgct gtacgccgag ggcgataagc
     5641 cggagcatat catgtgtctg ctgcgcggta agatcaaaat taccaaaaaa ggcgttggcg
     5701 gacgcaacca tattgtccgt atggtcaggc ccggaggcta ttttgcctat agagcttctt
     5761 tggccggaac gatgcattcc acttctgcta cggccttcga agcttcggat gtgggtgtat
     5821 ttccttccgg attgttcatc aagctgttga ggcaaaacgg cgagctggct ttttacttta
     5881 tcaaagcact ggctgtcgac ctgggctttt ccgactcccg ccaagtgaat cttacccaaa
     5941 aacatatccg cggacgtctg gccgaatcgc tgctgttcat gcgcaatcat tacggcctgg
     6001 acgaagacgg cgccaccatc aacatctacc tttcgcgcga agacctggcc aatctctcca
     6061 acatgaccac ctccaacgcc atccgtaccc tgtcgatgtt tgtatccgaa aaagtcatca
     6121 ccctcgacgg ccgccgcatc aaaatcattg atgaagacaa actggagaga atcagtcgct
     6181 taggttgatt ctttgtcgtt attattcttt gctgttaatt tttactgctc gttttcgcag
     6241 ctccccggtg tttctcttgc ttaaaatgtt taagccttta tccagtggat tttaaggcct
     6301 cgccgctcca tgcccctgaa ccaggtcatc tgccttttgg caaattgatg tatggctgtt
     6361 tcgagctgtc ggtacatttc gtcgtaactg agctcaccca acacataaag cgtaagatac
     6421 ttgtattcca ggccgtaata aagaaggtca tcggctgcaa ctccgctttg aagcaagttt
     6481 ctcacttctt ctaccatgcc ggaatcgagc ctggcctgca atcgttggct gatgcgacgc
     6541 cttcgttctt cgcgttccac ctccaaacct atgatatagg gctgcaaagg gggcaaatcg
     6601 gattttattt cgggatgagt agccaggtat tcggcaattt ctatggctct gagggctcgt
     6661 ttgggggtgt cggtgtcgct ggtgttgtgc aggtttttgt agccggccag tatgtcgctg
     6721 agttcggcca gggattttcc ttcgagcgaa gctctcaggg cgggattttc gggtacggcg
     6781 ggcaaggtat aacctttaag tacggcttcg atgtacaagc cggttcctcc gcagagtatg
     6841 ggcaatttgt tccgggaacg gatatcccgc cagacccggg taaaatcgcg ctggtattcg
     6901 tataaattat agcggctgcc tgcggggcaa atatcgataa ggtgataggg aattttctgt
     6961 ccgccgggca atgtatattc cgacaaatct ttgccggtac cgatgtccat gccgcggtaa
     7021 acctgccgtg aatcggcgct gatgatctcc gcattcagcc ggccggccat ttcgacggca
     7081 aatttggttt tgccgccggc tgtggggcca agtattacaa gtagattgca ttcgttgggc
     7141 aactgcatgg gattggggga cttttgcggg caaagataac aagaaaatca agagcgctcc
     7201 ccactgactg caagttacag cccggctgat tgatacccgg cagtgcatat caggattttt
     7261 ggcagcgctc ccctccactc aagcccaaac cgctcagcct cccgatatcc acggctggtc
     7321 agcgctggac gccggcgttg cctgtttgtc tctgcgagcc tgcacaggca aattttgccg
     7381 gcctctgcgc gcttcgggcg ccgtgtctat atatcggctt cgggtctgga ctttcgttgc
     7441 ggtacgcttg cgggagattc tatttctccg taagaaacta cgacgatgaa tgaagaacag
     7501 acatcattca aaataccccg tcctatccaa aaactccctc tgattgtttt ctctgcggat
     7561 ggttagggtg cctgtggttt cggggccgtt tttcagggtc ttttcgttta tgcgcactgt
     7621 ttcgcggggg cctctgtctt tgttcaggtt ccacatgtat tctccctggg cgtccatgta
     7681 aatgcgcttc atggtcagtt taagttcgtt gccttttacc cgggctacca gataatcctg
     7741 ggtgttgaca atttctctgc cccaggatga gccgtggatg atttgccaga tgccgtcgga
     7801 ttccaatacg gtgacgtcgt gaaattctcc tcccaggtag gcgtccactt cgtgtttttt
     7861 catcagttgg taaaactcgc tttctttgcc gccttccaac ataagggcgc tcgaggaacg
     7921 tctgttgatt tcgccccata agcctacgtg gccctggacg atcttgaatt tggcgtcgct
     7981 gtggtcttcg agcacttgtt cgaaccattc gagttgttcg ccacaaacag ttatgcgcat
     8041 ggaatcgtct atcacttcga acgtttctac ggtaacgatc aataaatcgc cttcacgaac
     8101 gtaatatgcc aggcctttct tgttttcggg gccgttttgc ggcatgcgca gatgctcggc
     8161 gtacactctc tcgaaacagg gtatcagctt gattttatgc tcgggccagg gatcgtctcc
     8221 caaatcgtga tcgcccaggg ccgtataata tttcaagccg tattcgttca tacgtctgac
     8281 ccaggccgaa taatagatgc tgcccatgtg ctccacacac ttggggctat cccaccagtg
     8341 gccgtttacc aaatcgccgg ctaccagcac aaaatgggga ttctcggctt ttacctgagt
     8401 caaaaagtaa tctacagcct cttcgtaacc cggccaggga ttggggatat cgaaattgaa
     8461 aaaatcggga aagcttacaa aacatacagc ttgcgctttt tttccccggt ttgtttgctc
     8521 cggcataatg aacgtgaccg ccaacatggt gatcaccagc gcgaaggcga ctgccgcaat
     8581 cagaaaccat ctgtttttgt ttgtactcat ggtatttggt attagaaggt ttagattaag
     8641 attttaattc agaatagagg aataaattaa gcggctctaa attactaata atttctgata
     8701 gtggcatata ccccatagga ttgatcttct atatcgaaca aaggaattag ccggtaggtt
     8761 ttaccgttgt tgttcaggcg aaaactgttg ctttctttat ctttcgacaa ctgttgcagt
     8821 atcgactcgt gattgccttt cataatcaac tcctcattgg aatcgaaggc cagcagcatc
     8881 gggccataga aaatggcgaa cacattgttg tcgtcaggca tggttttaag ataaaagtcg
     8941 tattgaaaga ccaattgtat tttgtctttg tttttccagg aacgatgcag gctgatatag
     9001 gaattgggcg ttgctttcgc ctcctgtttc tcgccgttaa cgtagacttc tgctttgcgc
     9061 gcccaggagg gaatgaaaag cttcagagta aggtcgattt tttttgcggt ggtaaggctg
     9121 aattcaacac aagtgtccgc agggaaatgg cctgtttgtt ccagaacaag ctttttttcg
     9181 tcccaatgca ctttcgaggg cacatacaag ttaacccaca aagccgtatc gttgcgataa
     9241 taaatgcctg tattcagggc cgaaaaagct tctatcgaag taccgttgca acagcggaaa
     9301 tcgttttccc tgagataggc tttttgccgg ggagaaccca aaggcaagtg ataaacacat
     9361 cttcccgttt tcgcgctttg caaggccatt acagcgttgt aaaaggtgtt catataggca
     9421 tcggcataaa ccggatcaag cttccaggta aaaagagtgg aactgagctt ttgggtattg
     9481 tgcgtgacgc aggtttccgc aatttccccg gtcatagtat tgctaagctg cccgggcact
     9541 ccccaatgtt ccgccaaaag cgaggtataa gtggtcacgt tgggacgagg gccgctgctc
     9601 gtaccgtttg catatacatg cgattccatc agcctgtccc aaaaataaac cacagcgtcg
     9661 tagtattttt tttctttagt gacagcataa cgctgagcat agccattcac cagcgctata
     9721 tgggtattgg cgtgcagccc cgacaatatg tcttcgtttt tcgataaagg aacaacaaac
     9781 caatccctgt cgaacaatgc tgccaacgcc agatgcccgg ggtctttcga aactttatac
     9841 aggttataca gcacttcgtt catggccccc gcctcattgg aaggattggc cccaacactg
     9901 tacaacattt tctctatggt gtcgtcgctg agtctcgaca ttcgtttttc cacatagtcg
     9961 gccatgccca aaagcatttc gtaggccttt gcattccctg tgcgggtata cacatccaac
    10021 agcccctgca taattttttg ataggtataa tacggagccc aaacgccccc aaaccgggtc
    10081 tccaacacat cgaaatcgct ctcgggaaaa gcgcttaaat aaccgttccc aaaactttgc
    10141 cagcatttat acaattcctc cgtcatataa ttcagccgct ccgccaacaa ctcatccccg
    10201 tatttatcca ccacaagagc cgccgccgac aaataatgcc ccgtaaaatg tcccctcaaa
    10261 cccgtcgtcg gcgactccca gccctccaag ggcagcgccg tcgaaggcaa acccgcatta
    10321 accctgaaat tatgcaaaag cctatccgga tcgagagatt tcaaatactc aatattcaga
    10381 tcctcccgcc gtttcaccca cgaatccgtc agcaccaccg acctctccgg aaaggaagag
    10441 gccttcccca cctccgaaac aaaagcctga tgccgacaat aca
//
LOCUS       DAOSTD010000067        10467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_6201_length_10467_cov_14.118901, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000067 DAOSTD010000000
VERSION     DAOSTD010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10467
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6201_length_10467_cov_14.118901"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1121)
                     /locus_tag="PLI37_05525"
     CDS             complement(108..1121)
                     /locus_tag="PLI37_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOQ58501.1"
                     /translation="MNNLFVYCEIEERQIAEVSLELLTKGRKLADELSCKLEAVVIGH
                     ELDEISSQVLPYGVDVLHLFDHKTLYPYTTLPHASILIKLFKEERPQICLMGATVIGR
                     DLGPRVSSALRSGLTADCTALEIGSHKERDKTYEELLYQIRPAFGGNIVATIVNPECR
                     PQMATVREGVMKKEILDPGYRGKVIKHKVEDYVSDSDFVVKVIDRHIEKSKNKLNSAS
                     IIVSGGYGVGSKENFQLLYDLAAVLNAEVGASRAAVDAGFCEHDRQIGQTGLTVRPKL
                     YIACGISGQIQHLAGMQESAIIISINSDRSAPINSIADYVVVGRVEDVIPKMIQYYKK
                     NTK"
     gene            1384..3213
                     /locus_tag="PLI37_05530"
     CDS             1384..3213
                     /locus_tag="PLI37_05530"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOQ58502.1"
                     /translation="MKAYYEHISSPPKHKSPCIGLSANFDDKQALLAKAYFEALALAG
                     GSPLIIPPFKGKEQVESLLDRLDAIIFTGGGDFDPALLGEEPHEALGSFNSVRDEFEL
                     RLLKLALDKQLPILGICRGIQLINLGMGGSLYQDIYSQRRKQCINHNQTGERSETSHY
                     VELMPDTLLRSLFNKDELAVNSFHHQAIKELAGGLKVCALSSDGIIEAVESDQGKAIL
                     GLQWHPECLVSHSKEPMLPVFNWLVKEAGLYRNCRDLCQSNITLDSHCDTALYFTKEQ
                     HIEVELYSPGFDFYSGISANNANGEALSSIPENKVDLRKMQNGGLDCAVMAAYIKQGS
                     RDRQGLEKAGQTCRNMLESIQKQVANYPHLAGLAQTAEDIRKLKSQGKKAILLAIENA
                     YALGTNLNLLKEFDKAGVCYITLCHNGHNDVCDSASDENNPEHHGLSPFGKELVKSMS
                     AMGILPDVSHASEKTFYDVLEYCPAPVIASHSSAKALCKHPRNLSDDQILALGKHKGY
                     IGICLYEGFLAKNRRAGIRDVIAHINHIRSLAGVEIIGIGSDFDGGGGIPGCQNAGEF
                     IHLLRALKAEGYRDEEIALITGGNFIRTLELAQLYAENLKKAP"
     gene            3213..4247
                     /locus_tag="PLI37_05535"
     CDS             3213..4247
                     /locus_tag="PLI37_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOQ58503.1"
                     /translation="MLLAPKTSQRLSLKEISVILSLAAVLFLLLLMTSCMSGQKKNSP
                     SEQNLKVVPVFNEDSAYHYLARQLDFGPRVPNTEGHKACGDYLVEKMRSLGGILTEQP
                     MQLVAFDGSVLRARNIIASFDPEKTRRILLFAHWDTRPWADEDPDPQNHTKAIPGAND
                     GASGVAVLMEIARHLGTTAPASLGVDIILFDAEDYGQPSFSGRSRVEDSWCLGSQYWA
                     KNPHVSNYSAEFGILLDMVGAKGAVFAKEYFSLRYAAPIVNKVWNTAARIGYSPYFEN
                     SEGGAITDDHLYINRLAGIPSINIIDYRSDTPNGFFPYWHTLEDSLDKIDKKTLKAAG
                     QTLLELIYTE"
     gene            4250..4906
                     /locus_tag="PLI37_05540"
     CDS             4250..4906
                     /locus_tag="PLI37_05540"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOQ58504.1"
                     /translation="MLLPFLQEGRIEAGCDEAGRGCLAGPVFAAAVVTGADFDCPEIN
                     DSKQLSYKERYRLRAEIEKRALAWGIGTASPEEIDKINILNAALLAMHRAVLHLQLSP
                     DYLLIDGNRYRKPSQLPIHKISVSVDEFSKIEHIPAGCFVKGDGRFVSIAAASILAKT
                     YRDDYMQHLHKQYPQYAWNKNRGYPTAEHRRAIADFGISPFHRKTFRLLPSEQQLSLF
                     "
     gene            4909..>6186
                     /locus_tag="PLI37_05545"
     CDS             4909..>6186
                     /locus_tag="PLI37_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOQ58505.1"
                     /translation="MAAIKDLTRGPIGKQLFYLSLPIISTSFIQMAYSLTDMAWVGRL
                     GPESVAAVGAVGILVWLTNSVSLLNKVGAEISVAQAIGTQDLDKARCYASHNVSISLI
                     ISLIWAGFLFAFSYPLLGIYKIEAAIELEAVTYLRIVLFGFPFLFLSATFTGIYNAAG
                     LSKIPFYINSIGLILNLVLDPVLIFALDMGTAGAALATLISQVVVCLLFFARIKLRDR
                     LLGSFPFFVRLQPDCVRKIVNLGLPVTLFNGFFAIINLLMGRISSIFGGHIGLMTLTA
                     GGQIEGLAWNTSQGFSTGLSAFVAQNYAAGKKSRILRAYRSGLAITTVFGLLLTLLFV
                     FYGGEVFTLFVPGQIAFDAGQLFLRIEGYTMLFMMVEITIQGVFFGTGRTLPPALISS
                     VFNLLRIPLAIILAKNGMGLAGIWWAISLTSLLK"
     assembly_gap    6187..6286
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6287..6944
                     /locus_tag="PLI37_05550"
     CDS             <6287..6944
                     /locus_tag="PLI37_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58506.1"
                     /translation="KTFYIFVFLVACFYSHGQTGEAIYSIINEEIVEKPKFIDSNPID
                     NSSAVEPYIPYGCDSVSTDYGSFCVKLSKYSGYNDEPGFNVVEIQRNNLTIFKLENSN
                     GYANLSSYVPSEHLNFSWVKLSPVRCALIFNGYIFASQPSMVSIVVIHKNEAKLVFNK
                     PLFIHSISKSTTDYFLNITLQSNTVEYGGTPESPVALGTPSLHTIWWDGNVLRYVKQR
                     "
     gene            complement(7017..7991)
                     /locus_tag="PLI37_05555"
     CDS             complement(7017..7991)
                     /locus_tag="PLI37_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58507.1"
                     /translation="MKKGILFIALGLFAYTMLSCDPVNGNGDGGDDPEAPKNYWERSA
                     LTHAQLRGPVKMVKEFYSDTTYKMTTFDEIGRILKIENTGQGNDFLTNYTYNTSGQLI
                     QAGTTTFEYADHGKYVPRGHFHMNFAGLAPNLSAINDERARTSYTFVGDTLFIINTVQ
                     YSDNADENTYVDTTKILYSGKYPVSSRTEHIFSEGNSWGEFMNASYQENGMFDVYSEG
                     FFGTGDNAYTSTRTYTYKKDDEFMLIDNYRYEDVYPGQEQPSSYTSNYTYNTQKDLTQ
                     IAEGEYIQEYSDYVYDTHNNWTSRKYRYKNGPEWSSYTDERREITYWE"
     gene            complement(8031..10217)
                     /locus_tag="PLI37_05560"
     CDS             complement(8031..10217)
                     /locus_tag="PLI37_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ58508.1"
                     /translation="MKNIVHILLLTMFFASSSQLSASQYLEGNKVPSANNNDLLTHGL
                     FRPNGSTSVYFQLIQENMLRSSNPEMPEPAYLNLYEPDLNLIKSFALRLPVEEAQLNY
                     WAGGLELVNSLVNDTLKQFLIYKFSVYDSAYVSQFTDENWSVFVGVLDESGDSLFTVL
                     SNFHGFETFEVNGQVKLAVNRVCSKDTAFFGGGSWFTTDVYTGQVYDLSTGALELQMP
                     HGIMYEGITFDEGQRRILGRNNNIAAAYEHLYKEYDLLLYNLDGSLYKTAVDSLSDYW
                     VGEFYHLSSVGQYCMKEKYSNTIYSLPDYEEWVSGGIFYDSQGLAKLVDYNNDTVRIY
                     NEDRSLYKSLAFEQDNMHSVVWVNMHDIVSDDKIEIVRECPNGGLQIFTEDGDTIFEN
                     WDLKLVENNDFSGGKVVLRFPDENYGRLIATDMNDFDYYTSYYKVGNLPVIAQMGDAP
                     GNDNIELLGLGEEGFIVLDTLELIDGRGAFVVGEGEYALRSQGKVQSETLRSLSTYLP
                     SELLWENAGIVSFTTDEIDALIISIQTVDLSVSAGASGQIRGTIRLIDNEGGLRSASL
                     PTYDLYLVASSDHSQVKSFTTSNQEGVYLFDRIPAGSYDVLLNVEGKAMTSVNTVVLS
                     SDASIVENVDYEITGEGIVATMQSSIERVRPKAPGSYLIHQGNTLLISGIEGTIQVRI
                     IDLSGRVLLNAQTNRGQVDVSGLQSGIYLLELQAGEQKQVLKFRKR"
BASE COUNT         2748 a         2460 c         2233 g         2926 t
ORIGIN      
        1 agcggcatcg ctgaaattac gttcttttag ttccattatc cgttgcatca acggatgctg
       61 caaataatgc ttaagtacag ggttgtcggt aaaataattg cccatggcta tttagtgttt
      121 tttttgtaat actgaatcat tttggggata acgtcttcca ctctgcccac aacgacgtaa
      181 tcggcaatac tgtttatggg agcggaacga tcgctgttga tggaaataat aatcgcgctt
      241 tcctgcatcc cggccaaatg ttgtatttga ccggagattc cgcaagcaat atacaacttg
      301 ggcctgaccg tcaggccggt ttgtccgatt tgccggtcat gttcacaaaa accggcatcg
      361 acggcggctc tggaggcacc cacttcggca ttaagcaccg cggccaaatc gtataaaagt
      421 tgaaaatttt cttttgaacc aacgccatag cctcctgaaa caataataga agcgctgttg
      481 agcttgtttt tgctcttttc gatatgacgg tctatcactt taacgacaaa gtcgctgtcg
      541 ctcacataat cttctacttt atgtttgatg actttgcctc tgtagccggg gtcgagtatc
      601 tcctttttca taactccctc ccgtaccgta gccatttgag gacggcattc cggattgacg
      661 atggtagcaa caatgttacc tccgaaggcc ggacggattt gatacagcaa ttcctcatag
      721 gttttatcac gttctttgtg acttccgatt tcaagggctg tacaatcggc tgtcagtccg
      781 cttctcaaag cagaagaaac tctggggccc agatcgcggc caataaccgt tgcgcccatc
      841 agacagatct gaggtctttc ttccttaaac aatttgatta agatcgaagc gtgaggcaag
      901 gtggtgtagg gatacaaggt cttgtgatca aacaagtgca aaacatcaac gccgtaaggc
      961 aaaacctgac tgctaatctc gtccaactca tgtccaataa cgactgcttc aagtttacag
     1021 ctgagttcat cggccaattt ccggcctttg gtgagcaatt ccaaactcac ttctgcgatt
     1081 tgtctctctt caatttcgca ataaacaaac aagttattca tgcctaaaag ggtataacta
     1141 aaacaatact ctgccggaga aaaacgcctc tccgattcaa ggcatgcaag attcacaatt
     1201 ttttgtcaaa tctacaagag atttgactat taattttccg cttggcgacg gctgttttga
     1261 ttttttatag aatttcggct tacagcaaaa tctcctgaca ccggtctttg taattgccgt
     1321 ctttgcttat ctttgcacag aaacaatcaa ctatttggtg caagcttcat ttacgaacac
     1381 tccatgaaag cctattacga gcatatttct tctcctccta agcacaaaag cccatgtata
     1441 ggcttgtcgg caaactttga cgacaagcag gctttgctgg ccaaagctta ttttgaagcg
     1501 ctggctctag ccggtggctc tccgctgatt attccgccct tcaaaggcaa agaacaagtc
     1561 gaaagtctgc tggacaggct ggatgccatt atttttaccg gcggaggtga ttttgatccg
     1621 gctctattgg gagaagaacc ccacgaggcc ttgggtagtt tcaattccgt ccgcgatgaa
     1681 tttgaattac ggctccttaa attggctttg gataaacaac ttcccatact cggcatttgc
     1741 agaggaatac aacttatcaa tttaggcatg ggaggctccc tttatcagga catctacagt
     1801 cagcgtcgca aacagtgcat caaccacaac caaaccggcg aacgatcgga aacctctcat
     1861 tatgttgagc ttatgccgga cactttactt cgttccttat tcaacaaaga cgaactggct
     1921 gtaaattctt ttcatcacca ggctatcaaa gaattggccg gaggactgaa agtatgcgcc
     1981 ttaagctcag atggtataat cgaagctgtg gaatcggatc aagggaaagc cattctgggc
     2041 ttgcaatggc atcccgaatg cctggtatca cacagcaaag agcccatgct gcccgttttc
     2101 aactggctgg ttaaagaagc agggctgtac aggaattgtc gcgatctgtg ccaaagcaac
     2161 atcaccttgg attcacattg cgacacggct ctctatttta ccaaagaaca acacatcgaa
     2221 gtggaattgt attcccccgg ttttgatttt tattccggta tcagcgctaa caacgccaac
     2281 ggtgaagctc tctcttctat tcccgaaaac aaagtggatt taagaaaaat gcaaaacgga
     2341 gggctcgatt gcgctgtcat ggccgcctat atcaaacagg gaagccgcga caggcaagga
     2401 ttagagaagg ccgggcaaac ttgccggaat atgttggaaa gcattcagaa gcaggtcgca
     2461 aactatcctc atttagccgg actcgcccaa actgccgaag atatcaggaa actcaagtcc
     2521 caaggtaaaa aagccatcct gctggccatc gaaaacgcct acgctctggg gaccaatctg
     2581 aacctgctca aggaatttga caaggccggc gtgtgttaca tcaccctttg ccacaacgga
     2641 cacaacgacg tatgcgattc cgcctccgac gaaaataatc ccgagcacca cggattgagt
     2701 ccgtttggga aagagctggt caaatccatg tctgctatgg gtatcttgcc tgatgtctcg
     2761 catgcttccg aaaagacctt ttacgacgtg cttgagtatt gtccggctcc ggttatcgct
     2821 tctcactcct ccgccaaggc cttgtgcaag catccccgca accttagtga cgatcagata
     2881 ctcgctcttg gcaagcataa gggttacata ggcatctgcc tttatgaagg ttttctggcc
     2941 aaaaaccggc gggcggggat cagggatgtt attgcacaca tcaaccatat ccgctcactg
     3001 gccggtgttg aaataatcgg cataggcagc gattttgacg gcggcggcgg catacccggt
     3061 tgccaaaacg ccggtgaatt catccattta ctgagagccc tcaaagccga aggctatcgt
     3121 gacgaagaaa tcgctctgat aaccggaggt aattttatca gaactctgga actcgctcaa
     3181 ctttatgctg aaaacctcaa aaaagcaccg taatgctcct tgctccaaaa acatcccaac
     3241 gattatcgct taaagaaatc agtgtcatac tttccttggc tgctgttttg tttttactcc
     3301 ttttgatgac ctcctgcatg tcgggacaaa aaaagaactc tccctccgaa caaaacctta
     3361 aggtggtgcc tgtttttaat gaagactcgg cctatcatta cctggcaagg caacttgatt
     3421 tcggtccccg ggttcccaat acggaaggac ataaggcctg cggcgattac ctggtagaaa
     3481 aaatgcgctc gcttggcgga atccttaccg aacagcctat gcaactggtg gccttcgacg
     3541 gcagcgtcct aagagccaga aacattattg catcgtttga tccggaaaaa acgcgaagaa
     3601 ttttactctt tgcccactgg gacacccgcc cctgggctga cgaagatccc gacccgcaaa
     3661 accatacgaa ggccattccc ggagccaacg acggggccag cggagtggct gtcttgatgg
     3721 agattgcccg gcatctgggg acgacagccc ctgcttcttt gggggtggac attattttgt
     3781 tcgatgccga agattacggg cagccctctt tttcaggcag atcgagggtg gaagattcct
     3841 ggtgccttgg ttctcagtac tgggcaaaaa atcctcatgt cagcaattat tccgctgaat
     3901 tcggcatttt gttggatatg gtcggtgcta aaggggctgt ttttgccaaa gaatattttt
     3961 ctctgcgtta tgcagcacct attgtcaaca aggtttggaa cacggccgca aggataggtt
     4021 acagccctta ttttgaaaac tcggagggtg gcgccattac cgacgatcat ctgtacatta
     4081 accgcctggc aggtatcccg agcatcaaca tcatcgacta ccgcagcgac actccaaatg
     4141 gatttttccc ttattggcat accctggaag acagtctgga caaaattgac aaaaaaaccc
     4201 tcaaagcagc aggccaaacc ctgttggaat tgatttatac cgaataaaca tgctcttgcc
     4261 ttttctacag gaaggacgta tcgaagccgg ctgtgacgaa gccggccggg gttgtctggc
     4321 cgggccggtc tttgcggctg cagttgtaac gggagccgac tttgactgtc cggaaatcaa
     4381 cgattccaaa caactcagct acaaagaacg ctatcgcctg agagccgaaa tcgaaaagcg
     4441 agcgctggcc tggggtatcg gcactgccag ccccgaagaa atcgataaaa tcaatatcct
     4501 caacgcagct ctcctggcta tgcacagggc tgtgttgcat ctacagctta gtcccgacta
     4561 cttactcatc gatggaaatc gctaccgcaa gccttcacaa ttacccatac ataagatatc
     4621 cgtaagtgtc gatgaattca gtaaaattga acatatcccg gccggttgtt ttgtaaaagg
     4681 agacggaaga tttgtatcca ttgctgcagc ctccatactg gccaaaacat acagagacga
     4741 ttatatgcag catttacaca agcaataccc tcaatatgcc tggaacaaaa accgaggcta
     4801 ccctaccgca gaacatcgcc gggccattgc agatttcgga atcagccctt tccatcgaaa
     4861 gacattcagg ctgttgccgt cagaacagca attaagtcta ttttaagtat ggctgcaata
     4921 aaagacttaa cccggggacc catcggtaaa cagctttttt atctctccct tcccattatc
     4981 agcacttctt ttattcaaat ggcttacagc ctgacggata tggcctgggt tggccgtctg
     5041 ggtcccgaat cggtagctgc cgtcggagct gtcggcatct tagtctggct taccaattcg
     5101 gtctctctgt tgaacaaagt gggggccgaa atcagcgtgg ctcaggccat aggaactcag
     5161 gatttggaca aggcccgttg ctacgcttcg cacaacgtca gcatctcgct aattatttcc
     5221 ctgatttggg ccggtttttt gtttgcattt tcctatcccc tgctgggtat ttataaaata
     5281 gaagccgcta ttgagctgga agccgtaact tacttaagaa ttgtgctttt cgggtttcct
     5341 tttctgtttt tgtctgccac cttcaccgga atctacaacg cggccggact gagcaaaatt
     5401 cccttttaca tcaacagcat cggtttgatt ttaaacttag tactcgatcc tgtacttatt
     5461 tttgccttag atatgggtac ggctggagca gccttagcaa cattgatttc gcaggtggtg
     5521 gtctgtctct tatttttcgc ccggataaag cttagggaca ggctattagg aagttttcct
     5581 ttttttgtcc ggctgcaacc ggactgcgtc cgtaaaattg taaacctggg gctgcccgta
     5641 acacttttca acggcttttt tgccattatc aatttattaa tgggacggat ctcatcgatc
     5701 ttcggaggac atattgggct gatgactctt actgccggag gccaaatcga gggcctggcc
     5761 tggaatacct cgcaaggttt cagcaccggt ctgagtgctt ttgttgccca aaactacgct
     5821 gccggtaaaa aatcgagaat tttacgcgca tatcgctcgg ggctggccat aaccactgtt
     5881 ttcggattgc tgttgaccct gttattcgtt ttttacggag gcgaagtttt taccctcttt
     5941 gttcccgggc aaatcgcctt cgatgcaggg caattgtttc tgcggatcga gggttatacg
     6001 atgcttttta tgatggtgga aatcaccatt cagggagtgt tttttgggac agggagaacc
     6061 ctaccccctg ccctgatcag ctccgtattc aatctgctcc gcatacccct ggccattatc
     6121 ttggccaaaa acggtatggg tcttgccggt atctggtggg ccatcagcct taccagcctg
     6181 ttgaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaag acattttaca
     6301 ttttcgtgtt tcttgttgct tgtttttatt ctcatggaca aacaggtgag gcgatttatt
     6361 cgattattaa cgaagaaatt gtcgaaaaac ctaaattcat tgattcaaac cctatagaca
     6421 actcttctgc agttgagcca tatataccat atggatgcga ctctgtcagc actgattatg
     6481 ggtcattttg tgtcaaatta tcaaaataca gtggatataa cgacgaaccc ggattcaatg
     6541 tggtagagat acaaaggaat aatttgacga tttttaaact tgaaaactct aatggatatg
     6601 ccaatctatc ctcttatgtg ccatcagaac atttaaactt ttcttgggtc aaattgtctc
     6661 ctgtgagatg tgctttgatt tttaatgggt atatatttgc gagccagccg tcaatggtga
     6721 gtattgtggt aatacataaa aacgaggcta aattagtatt taacaaaccc ctgtttatcc
     6781 attctatttc aaaaagtacg actgattatt ttttaaatat tactctccag tcgaacactg
     6841 tcgaatacgg aggaacaccg gaatcccccg tagcgctggg gactccttcg cttcacacaa
     6901 tttggtggga tggaaacgtt ttacgttatg tcaaacagcg ttagccgtga acttttcgag
     6961 gttcacggct aacgccggca cttaaaagta tctgttaaca gatcaaagga agattcttat
     7021 tcccaataag taatttcgcg acgctcatcg gtataactgc tccactcggg tccgtttttg
     7081 taacggtatt tacgactggt ccagttgttg tgagtatcgt atacgtaatc ggaatattct
     7141 tggatatact cgccttcggc tatctgagtc aggtcttttt gtgtattgta agtataatta
     7201 cttgtgtagg agcttggttg ttcttgaccg ggataaacat cctcataccg gtaattgtca
     7261 atcagcatga attcatcgtc tttcttgtaa gtgtaagttc ttgtacttgt ataggcgtta
     7321 tcaccggtcc caaagaaacc ttcggaatag acatcaaaca ttccgttctc ctgataactg
     7381 gcattcataa attctcccca ggaattacct tcagaaaata tatgttctgt tctggaagac
     7441 actgggtatt ttccggaata aagaattttg gtagtatcca cataggtgtt ttcatcggcg
     7501 ttgtcactgt attggacggt gttgatgata aatagggtat ctcccacaaa cgtataactt
     7561 gtgcgggctc tctcatcatt aattgcggaa aggttaggag ccaaaccggc aaaattcata
     7621 tggaaatgac cccttggaac atacttgccg tggtcggcat attcgaaagt agtagttccg
     7681 gcctgaatca attgtcctga ggtattgtac gtataattgg tcaaaaaatc attgccctgc
     7741 cctgtgtttt caatcttaag aattcttccg atttcatcga atgtggtcat tttgtaagtg
     7801 gtgtctgaat aaaactcttt gaccattttc acaggccctc tcaattgggc atgcgtaagg
     7861 gctgaacgct cccaataatt tttgggagct tcgggatcat cccctccgtc cccgtttcca
     7921 tttaccggat cgcaagatag catcgtataa gcaaataagc ctaatgctat aaacaatatt
     7981 cctttcttca tgattaaaaa tttaattata attgaatact aattttgata ttatcgtttt
     8041 ctgaatttga gtacttgttt ttgctctccc gcttgtagtt cgagcagata aatgccgctt
     8101 tgaagcccgc taacatccac ctgaccccgg ttcgtctggg cattgagtaa taccctgccg
     8161 ctcaagtcga taattcttac ttgaattgtc ccttcaatac cgcttatcaa caaggtgttg
     8221 ccctgatgta ttaaatagct tcccggtgct tttggacgga ctctttctat gctgctttgc
     8281 atcgtagcca caatgccttc tccggtaatt tcgtaatcca cgttttctac gatgctggca
     8341 tcggacgata agactacggt atttaccgaa gtcatggctt taccctccac attcaacaac
     8401 acatcgtagg aaccggcagg tatgcgatca aaaagataaa ccccctcttg gttactggtg
     8461 gtaaaagatt tcacctgact atggtctgaa gatgccacca aatacaaatc ataggtcggt
     8521 aaagacgcac tgcgcaaacc gccttcatta tcgatcagac ggattgttcc tctgatttgc
     8581 ccgctggcgc ctgcgctgac agataaatca accgtctgaa tgcttataat taatgcatct
     8641 atctcgtccg ttgtgaacga cactataccg gcattctccc aaagtaattc gcttgggaga
     8701 taggtactca aactgcgtag agtttccgac tgtactttcc cctgactgcg caaggcatat
     8761 tctccttctc caaccacaaa cgctccccgg ccgtcgatca attccaaagt atcgagtaca
     8821 ataaatcctt cctcgcccaa gcctaataat tcaatgttat cgttccccgg agcatcaccc
     8881 atttgggcaa taacgggaag atttccaact ttatagtagc tcgtgtaata atcgaaatcg
     8941 ttcatatcgg tggctatgag ccggccataa ttttcatccg gaaaacggag tacgacttta
     9001 cccccggaaa aatcattgtt ttcaaccagt tttaaatccc aattttcgaa gatcgtatct
     9061 ccgtcttctg tgaatatttg cagacccccg ttgggacatt cgcgcacaat ttcaatttta
     9121 tcgtcactta caatgtcgtg catatttacc caaacaacac tgtgcatatt gtcctgttca
     9181 aacgccaggc ttttgtacag gctacgatct tcgttataaa ttcggacagt gtcgttgtta
     9241 taatcaacca gtttggccag accctggcta tcgtaaaaaa tcccgccgga aacccattct
     9301 tcgtaatcgg gcagggagta tatggtgtta gagtatttct ccttcatgca gtattgtccg
     9361 accgaagata aatggtaaaa ttctccgacc cagtaatccg acagactgtc aacggccgtt
     9421 ttatataatg agccgtccaa attgtataaa agcaaatcgt attctttata caaatgctcg
     9481 taggcagcgg caatgttgtt gtttctgccc aaaatacgcc tttgtccttc gtcaaaggtg
     9541 atgccttcgt acattattcc gtgcggcatt tgcaattcga gagctccggt ggataaatcg
     9601 tatacctgcc cggtgtaaac atcggtggta aaccagcttc ctcccccgaa aaaagcagtg
     9661 tctttggaac agactcggtt cacagccaat ttgacctgcc catttacttc gaatgtttca
     9721 aagccgtgaa aattacttag aacggtaaat aacgaatctc cactttcatc gagcacaccg
     9781 acaaagacgc tccagttttc gtctgtaaat tgcgaaacat acgcactgtc gtatacagaa
     9841 aacttataaa tcaaaaactg ctttaatgtg tcgttgacca ggctattaac caactcaagg
     9901 cctccggccc agtaattcaa ttgtgcctcc tcaacaggca gtctcaatgc aaaactctta
     9961 atcaggttaa ggtcaggctc atataagttg aggtaagcag gctccggcat ttcgggattt
    10021 gaactacgta acatgttttc ctgtatcaac tgaaaataaa cagacgtaga gccgttgggg
    10081 cgaaataggc catgcgtcaa taaatcgttg ttattggccg agggcacttt gttgccttcc
    10141 agatactgag atgcggaaag ctgactgctg cttgcaaaaa acattgtcag cagcaaaata
    10201 tgtactatat ttttcataaa gccgtattaa agtatgatta aaattggatt cttcctaaga
    10261 gtctcttttt tcattgaaaa gcaaaattat aacttggagc cctgagtgcc taggcaaaag
    10321 gtaaaattga ggccgtaaaa gatacatttt tcgacgcaaa atacatactt ttgaaaacaa
    10381 atcaaagttt atgcttaaaa cgattttatt actgactccc gtgtatgtaa ccttgttttg
    10441 gactttagtt ctgaaaacgg caaacag
//
LOCUS       DAOSTD010000068        10314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_6321_length_10314_cov_13.730091, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000068 DAOSTD010000000
VERSION     DAOSTD010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10314
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6321_length_10314_cov_13.730091"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..642
                     /locus_tag="PLI37_05565"
     CDS             40..642
                     /locus_tag="PLI37_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOQ58509.1"
                     /translation="MIDTYKTIANRSEGLYKDKGSKFLAFAVPVEGIEEVNLELEQFR
                     KSYFDARHVCYAYMLGVDGEQYRVYDDGEPSGTAGRPILGQIRSFGLSNVLVVVVRYF
                     GGILLGTGGLIVAYKTAAAAALSNARILEKTLDAQISLSFEYPYTNEVMSIIKDCEAQ
                     IIDPKYEELCSMSLSIRQSRLNELKERLSKVESVLITNDE"
     gene            635..1207
                     /locus_tag="PLI37_05570"
     CDS             635..1207
                     /locus_tag="PLI37_05570"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HOQ58510.1"
                     /translation="MNKDFFRRIWRIIVSPAATWKEITTETAENKPYLSAYFYPLLTL
                     VGMAAFFSSFIHTQETFRTELVQGIRIFIRVFAAFFASYFLSSFVLDRLFVRWLGLAP
                     DKIKTEKLCVYSLTPLLLVRFFTFLVKDLFFLYIFVVYTVYVVWEASGGLYEMPGKQR
                     GRFTALTTAVIILSPVLIELILIKLLPGLS"
     gene            1207..1668
                     /gene="smpB"
                     /locus_tag="PLI37_05575"
     CDS             1207..1668
                     /gene="smpB"
                     /locus_tag="PLI37_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOQ58511.1"
                     /translation="MKLSRDILIKNKRAAFNFELLDRFTAGIVLTGTEIKSIRLGKAS
                     LSDSYALMIKGELWIKNLQISEYDNGSYNNHNPRRDRKLLLTKRELRKIERLTRETGF
                     TVVPLKLFINEKGWAKLEIAVAKGKKAHDKRESIKEKDDRRQMDRYIKSKL"
     gene            complement(1680..2822)
                     /locus_tag="PLI37_05580"
     CDS             complement(1680..2822)
                     /locus_tag="PLI37_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ58512.1"
                     /translation="MKIIEVNTPALRKEFALLPFRLYRGNPYWVPPILKSEINNINPA
                     VNPFFRFCDAKFWIVQKNGLTVGRIGAIIHYAYIEKTQEKYGRFTRAEFIDDKTVVDA
                     LFETAENWIKSKGMNGAMGPLGFSNLDSQAMLIEGYDQLASVASVYHLPYYKDHLERL
                     GYRKKTDWIEFRLSLTSEIPEKAVRVADIIQQRYQLSVIRMKSKRIMRKFGRQIFRLF
                     NKSFYELFSFAPLDEKMIDHVLDTYLPVINPEYIHIVVNNEQKIVGFIVPVPSLSKSM
                     QLAQGKLSLRAIWSIMRSRKKNDTVDLFLTGIDPEYQAKGVTSLLITKAQEVMIKNGI
                     TTVETTGILEDNFKAIQHWKNYEHVQNKRRRCFIKDFDKQESESEA"
     gene            complement(2925..3767)
                     /locus_tag="PLI37_05585"
     CDS             complement(2925..3767)
                     /locus_tag="PLI37_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOQ58513.1"
                     /translation="MKQIAIQGISGSYHDIVAHHYFEGEEIEILGCKTFKDVFATMRK
                     DSSILGVIAIENTIAGSLLQNHNLLRESETAIIGEFKLRISHCLAAIPGQSFKDIREV
                     HSHPIALMQCDDYLGLHPEWTIVESEDTATSAKTIGNQKIMGRAAICSRQAAEIYGLE
                     VLEAGIETNKHNFTRFLVLADRWDAEKFNANNKINKSSMVFSTYHREGALSQVLSVLS
                     YYHNNLTKIQSIPIIGHEWEYLFFVDLTFEDRLKYKQSLEAIRPLTSNLKILGEYPVG
                     RQML"
     gene            complement(3795..4130)
                     /locus_tag="PLI37_05590"
     CDS             complement(3795..4130)
                     /locus_tag="PLI37_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOQ58514.1"
                     /translation="MNSPYFLMEKELPWTDLGGGVKRQILGYNQDIMMVKVAFEKGSI
                     GAAHKHPHTQSSLCVSGKFEVDIEGQRSVVSAGDGFYVAPDLVHGVRCLEAGMLLDAF
                     APCRKDFLP"
     gene            complement(4123..>4579)
                     /locus_tag="PLI37_05595"
     CDS             complement(4123..>4579)
                     /locus_tag="PLI37_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOQ58515.1"
                     /translation="MEEVTVEAIFRPDADGSFEQRFLHLGEISGERIMLETRVKPDGN
                     WYFDAYVKRPDGEGCALIDTNLIHPTEQWYHVAFVCRKDSLFSYVNGQFEKSAAFAYQ
                     TINKGISSVGVRQNKVCFFKGQMYRIRITPRALRPEEFLQDHLILNSYE"
     assembly_gap    4580..4679
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4731..6368)
                     /locus_tag="PLI37_05600"
     CDS             complement(4731..6368)
                     /locus_tag="PLI37_05600"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOQ58516.1"
                     /translation="MTLFLVTSAGLRAETKASSEDEVKTCMRKATAFMMDKVSINGGF
                     VSSYLSDFSRRWGELEAYPSMIWIDGPTPAMANVLLDAYLATEDEYYYRQACRVADAL
                     IAAQLPCGGWNYTFDFAGEESLKQWYATVGKNAWGVSEYNYYYGNATFDDQNTYRPAN
                     FFLRIYLLKKDPRHKEVMERAIDFFLKSQYPNGGWPQRYPLRYDFPAKDGSPDYSSFI
                     TLNDGVMEKNIDFMLRCYYLLDDERCLSAARKGMDCLLSLQQAPPTAGWALQYNLDLQ
                     PAKARDYEPASVCVSTTSGTVMLMMEYYRLTGEKKFMQGIPAALRFLDEVAFCDSMLQ
                     LIERSEEKVGRSSENGKLCPRMVVPGSSFTPLFLHRRGSHIVNGDYYIDQNPLNTIAH
                     YSSFCTINTDVLRSRYEDLLNLPAEEISRNSPLLHPELREPLLQQFLYNKRPEGMRRF
                     LYIDQTRPVQEVLASLNEEGYWPTPLVLTTNPYIGEGSADDPVPELYEETMKHPFNTQ
                     FYRSENPPLGISVNAYVQHMGVLIQYLLGQKENSNEK"
     gene            complement(6394..8463)
                     /locus_tag="PLI37_05605"
     CDS             complement(6394..8463)
                     /locus_tag="PLI37_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOQ58517.1"
                     /translation="MSIILSCFGCTNRQAAFKEFDYKVDRFADMEIIRYQVPGFEDLS
                     LRQKKLVYYLSEAALWGRDIIYDQNYRHNLLIRHCLEAIYQYADISRQGEDWQAFEVY
                     LKRLWVANGIHHHYSTDKFEPGFSQDFFVTALRAVPAEKLPSMESGRLTQEQNPLQTQ
                     DSLNTTQYADELAARLLPLIFDARLDAKRVNQAQGEDLIVSSACNYYRDLSQKEAEAF
                     YAVLNNPENPCPVSYGLNSRLVKEEGQLREEVWKIGGLYSPALEKITYWLELALEVAE
                     NPRQKEIIQSLIDYYHSGDLETFDQFSILWVQDLESKVDFINGFIESYGDPLGIKGSW
                     EAIVNFKNEEASKRTQIISDNAQWFEDNAPIADEFRKKEVKGVSAKVITNAIMGGDAF
                     PTTAIGINLPNADWIRRDYGSKSVTIENITEAYFQSSQGSGFNEEFMWSEAEVKASAK
                     YGFITGNLHTDLHECLGHGSGQLLPGVDPNALRSYGSVIEETRADLFALYFLADPKLV
                     ELGLLPNEEAYKAEYYEYVMNGLMTQLSRIEKGKQIEESHMRNRALIARWLLDQGSKD
                     KVMKLKKRHNKTYLVIHDYEALRQLTGELLAEVQRIKSEGDYTAAEALVENYAVQVDP
                     LLHQEILDRYAKLNLSPYKGFVNPLYKPVYNDKDEIIDIIVLHNENYVEQHLRYSKDY
                     AALPVEN"
     gene            complement(8500..9579)
                     /gene="aroC"
                     /locus_tag="PLI37_05610"
     CDS             complement(8500..9579)
                     /gene="aroC"
                     /locus_tag="PLI37_05610"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOQ58518.1"
                     /translation="MNTFGQLFRLSSFGESHGRGIGGVLDGCPPGLTVDLELIRKELS
                     RRRPGQSALSTNRNEADEVELISGIFEGKTTGAPIAFLVRNTDMRSQDYDYLKGIFRP
                     SHADYTYHSKYGIRDHRGGGRSSARETIARVVAGAFAKMLLNTMNISIEAFTSQIGPI
                     ALEQQDSTLFSSELAAENPLHCPDREKAEAMMALIARVKEEQDSIGGVVSCVVRGLPV
                     GWGEPVFGKLHARLASAILSIPACKGFEYGSGFDVSQKGSQQNDAFYFDGERINTRSN
                     HSGGIQGGISNGQPVYFRAAFKPIASIRIPQETVDKEGAAVKIQIQGRHDPCALPRVL
                     PVVEAMAALVLADACLLAKSKERIV"
     gene            complement(9597..10160)
                     /locus_tag="PLI37_05615"
     CDS             complement(9597..10160)
                     /locus_tag="PLI37_05615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOQ58519.1"
                     /translation="MKITDNKFVAASYDLYVGGEDGQAPELMERATEKEPLTFIFGTG
                     TMLESFESKLKNLQAGDSFDFVLPCDTAYGEYDEERVADLPKSIFEVDGKFDSENIAV
                     DKIVPMMTTEGQRLDGIVLEITDEFVRMDFNHPLAGEDLHFVGKVLEVREATDEEMEQ
                     LMNSCAGCGHDCSEGQSSNGSCRGCGC"
BASE COUNT         2486 a         2359 c         2529 g         2840 t
ORIGIN      
        1 cttagacgac tgaaaggtgc tgaacgcctg cattgtacca tgatcgatac atacaaaacc
       61 atagccaatc gcagcgaagg cctctacaaa gacaaaggca gcaaattttt ggccttcgcc
      121 gttcctgtag aaggaattga ggaagtgaat ctggagttgg agcaattcag aaaaagctat
      181 tttgatgccc gtcatgtgtg ctatgcctac atgctgggag ttgatggaga gcaatacagg
      241 gtttacgacg acggagagcc ttcgggaact gccggacgtc cgattttagg ccaaatccgt
      301 tcttttggtt tgagcaatgt cctggtggtg gtggtgcgtt attttggagg tattttattg
      361 ggaaccggag gtctgatcgt tgcctacaaa acggccgccg ccgcggcact ttccaatgcc
      421 agaatcctgg agaaaacgct ggatgcccaa atcagcctta gttttgagta tccctatacc
      481 aacgaagtga tgagcattat caaggattgc gaagctcaaa tcatcgatcc caaatacgaa
      541 gaactttgtt cgatgagttt gagcatcagg cagagtagat tgaatgaact gaaagaacgt
      601 ttatcaaaag tggaatcagt gcttataacc aacgatgaat aaagattttt tccgtcgcat
      661 ctggcggatt atagttagtc cggctgctac ctggaaggag ataacaacag aaacggccga
      721 aaataagccc tatttgagtg cttattttta tcctttgctg acactggttg gtatggccgc
      781 ttttttcagt tcgtttattc atactcagga aactttccgg accgaactgg tacagggtat
      841 tcgaatcttt attcgggttt ttgcggcttt ttttgcttcc tactttctat cctctttcgt
      901 actggatcgt ttgtttgttc gttggctggg cttggctccg gacaaaataa aaaccgagaa
      961 gctctgcgtc tattcgctga cacccttgtt gcttgtaagg ttttttacgt ttctggtcaa
     1021 ggatttgttt tttctgtata tttttgtcgt ttacacggtg tacgtcgtat gggaggcatc
     1081 cggcgggctg tacgagatgc cgggcaaaca aagaggtcgc tttacggctt tgacaactgc
     1141 cgtaataata ttgtctcccg tgctgattga attaattctt atcaagttgt tgccgggtct
     1201 atcttaatga aattatccag ggacatattg atcaaaaaca agcgggctgc ttttaatttt
     1261 gaattgctgg accgcttcac ggccggtata gtcctgaccg gcaccgagat taaatccata
     1321 cgcctgggca aggccagtct ttcggacagc tatgccctga tgattaaagg tgaactctgg
     1381 attaagaatc ttcagataag cgaatacgat aacggcagct acaacaacca caatcccaga
     1441 cgcgaccgca agctgctact gactaaaaga gaattgcgca aaatagaacg acttacccgg
     1501 gaaacgggct tcaccgtcgt tccgttaaag ctcttcatca acgaaaaagg ctgggccaaa
     1561 ttggaaatcg cagtggccaa gggtaaaaaa gcccacgata aacgcgagag catcaaggaa
     1621 aaagacgacc gtcgccagat ggatcgttac atcaaaagca aattgtaaaa tacaacagtt
     1681 taggcttcgc tttcagactc ctgtttgtca aagtctttta taaagcaacg tctgcgtttg
     1741 ttttgtacgt gctcgtaatt tttccagtgt tgtatggctt tgaaattgtc ttcgagtata
     1801 cctgtggttt cgaccgtcgt aattccattc ttaatcatca cttcctgagc tttggtgata
     1861 agcaggctgg tgactccttt ggcctgatac tccggatcga tacccgtcag aaaaagatct
     1921 acggtgtcgt tctttttacg gctgcgcatt atagaccata tagcccggag actcaatttg
     1981 ccttgggcaa gttgcatgga tttgctcagc gaaggcaccg gcacaatgaa accgactatt
     2041 ttttgttcgt tatttacaac gatgtgtatg tactcgggat tgattaccgg taggtaagtg
     2101 tccagcacat ggtctatcat tttttcgtcc agaggagcaa aagaaaagag ttcgtaaaaa
     2161 gacttgttga acaatcgaaa aatttggcgg ccgaattttc tcatgatgcg cttggacttc
     2221 atgcgaataa cgctgagttg atagcgttgt tgtatgatgt ccgccacccg gacagccttt
     2281 tcgggaatct cggatgtcag gcttaatcga aactctatcc agtcggtttt ttttctgtag
     2341 cccagacgct ccaaatggtc cttgtaataa ggcagatgat aaaccgatgc cacagaggca
     2401 agttgatcat agccctcgat gagcatagcc tgggagtcga gattcgaaaa gcccagaggt
     2461 cccattgctc cgttcatacc cttggatttt atccaatttt ccgctgtctc aaacaaagcg
     2521 tcaaccactg tcttgtcgtc gatgaattcc gctctggtga aacgtccgta tttttcctgg
     2581 gttttttcga tgtaggcata atggatgatg gcaccaatgc gcccgaccgt aaggccgttt
     2641 ttttgaacaa tccaaaattt ggcatcgcaa aaacggaaaa acggattgac agccgggttg
     2701 atgttgttga tttctgactt taagatagga ggcacccaat aagggtttcc ccggtacaaa
     2761 cgaaaaggca ggagcgcaaa ttctttacgc aaagccggag tgtttacttc tataattttc
     2821 atttttaact cttttaattt cctcttaata atcaacaaat atactacatc gggcttaaac
     2881 ttaccgcttt tagacgtgaa tttaaataag cacagtattt ttattcaaag catttgcctg
     2941 cccaccgggt attcacccag gattttcagg ttgcttgtca aggggcgtat cgcttcgaga
     3001 ctttgcttgt atttgaggcg atcttcgaaa gtaaggtcga caaaaaacaa atattcccat
     3061 tcatggccaa tgataggaat cgattgaatt ttggtcaggt tgttgtggta atacgacaac
     3121 accgacaaaa cctgagataa ggctccctcc cggtggtaag tggagaaaac catcgaagat
     3181 ttatttatct tgttgttggc gttgaatttt tcggcatccc aacgatcggc caaaaccaaa
     3241 aaacgggtaa agttgtgttt gttggtctct atgcctgctt ccagcacttc aagtccgtaa
     3301 atttcggcgg cctggcggct gcaaatggca gctctgccca taattttttg attgcctatg
     3361 gttttagcac tggtggcggt atcttcgctt tccactatag tccattcggg atgtaacccg
     3421 agataatcgt cgcattgcat cagagctatg gggtgagaat gcacttcacg tatatctttg
     3481 aagctttgtc cgggtatggc ggccaggcaa tggctgatcc ggagcttgaa ttctcctata
     3541 atggcggttt cgctctctct tagcaggttg tggttctgga gcaggctacc ggctatggta
     3601 ttttcgatgg caatcacacc caatatactg ctgtctttgc gcatggtagc aaacacatct
     3661 ttgaatgttt tgcatcccag tatttcaatt tcttcaccct cgaagtaatg gtgtgccact
     3721 atatcgtggt aagagcccga aatgccttgt atggctattt gcttcatatc attttttgtg
     3781 tgcaaatccc ggcattaagg caaaaaatct ttgcggcagg gagcaaaagc atccaggagc
     3841 atgccggctt cgagacaacg gactccgtgc accagatcgg gtgcaacgta aaaaccgtct
     3901 ccggcagaca caacggagcg ttgcccctcg atatccactt cgaatttgcc gctgacgcaa
     3961 agcgacgact gggtatgcgg atgcttgtgt gctgctccga tgctgccttt ttcgaaggca
     4021 acttttacca tcataatgtc ttggttgtaa ccaaggattt gtcttttaac tcccccgccc
     4081 aaatcggtcc agggtagctc tttttccatc aaaaagtaag gactattcat aactattcag
     4141 gattaaatga tcttgtaaaa attcttcggg tctaagagcc ctgggtgtaa tacggatgcg
     4201 gtacatttgt cccttgaaaa aacaaacctt gttttgacgc actcctacag agctaattcc
     4261 cttgtttatg gtttgatagg cgaaagccgc cgacttttca aattgaccgt ttacatagga
     4321 aaacaggctg tctttgcggc agacaaaggc aacgtgatac cactgttctg tcgggtgaat
     4381 aaggttggta tcgatcaagg cacagccttc tccgtcggga cgttttacat aggcatcgaa
     4441 ataccagttt ccatcgggtt ttaccctggt ctcgagcata atgcgttcgc cggagatttc
     4501 gcctaggtgc aaaaagcgtt gttcaaacga tccgtcggca tccggacgga aaatagcttc
     4561 gaccgtcact tcttccatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     4681 cctgtttgga ttgacaggcg ctcagcagga gtatgagcaa gagataaaga ttatttttca
     4741 tttgagttct ctttttggcc gagcaggtat tgaatcaaaa ctcccatatg ttgcacatag
     4801 gcattgaccg agatgcccaa aggtggattt tcggaacgat aaaactgcgt gttgaaggga
     4861 tgtttcatgg tttcttcgta cagctcgggc acagggtcgt cggcactgcc ttcgcctatg
     4921 taaggattgg tcgtcaacac caggggagtg ggccaataac cttcttcgtt taacgaagca
     4981 agaacctcct gaacaggcct ggtctggtct atatacagaa atctgcgcat tccttccggc
     5041 ctcttgttgt acaggaattg ttgaagcaaa ggctctcgga gttccggatg caagagagga
     5101 gaattgcggc ttatctcttc ggcgggcagg ttcagcaggt cttcgtatct gctgcgcaaa
     5161 acgtcggtat ttatagtaca gaacgaagag taatgagcaa tggtgttgag ggggttttga
     5221 tcgatgtagt aatcgccgtt tacgatatgc gagcccctgc ggtgcaaaaa cagaggcgta
     5281 aagctgctgc cggggaccac catgcgggga caaagcttgc cgttttcgct gctgcgtcca
     5341 actttttcct cgctgcgttc gatcaattgt aacatgctgt cacagaatgc aacttcgtcc
     5401 aaaaaacgaa gggcggcagg aatgccttgc atgaattttt tctctccggt caggcggtag
     5461 tactccatca tcagcatcac tgtgccggag gtggttgaaa cgcaaaccga agccggttcg
     5521 taatcgcggg ctttggccgg ttgtaagtcc agattgtatt gcagagccca gccggcggta
     5581 ggaggtgcct gctgcagcga tagcaggcaa tccatgccct ttcgggctgc agagagacag
     5641 cgctcatcgt cgagcaggta atagcagcgc aacataaaat cgatgttttt ttccatcact
     5701 ccatcgttga gggttataaa actcgagtag tcgggactgc cgtctttagc cggaaaatcg
     5761 tacctgaggg gatagcgttg gggccagccg ccgttgggat attggctttt gagaaaaaaa
     5821 tcaatagctc gttccatgac ttctttgtgc ctgggatctt tctttagcaa ataaatacgc
     5881 aaaaagaaat tggccggtct gtaggtattc tggtcgtcga aagtagcgtt gccgtagtaa
     5941 taattgtatt cactcactcc ccaggcgttt ttgccgacag tggcgtacca ttgtttgagc
     6001 gattcctcgc cggcaaaatc gaaggtgtaa ttccagcccc cgcaaggcaa ttgagctgct
     6061 attaaagcat cggccacccg gcaggcttgc cggtaatagt attcgtcttc ggtggcaagg
     6121 taagcgtcca acaggacatt ggccatggcc ggggtggggc cgtctatcca gatcatgctg
     6181 ggataggctt cgagctctcc ccaccggcgg gagaaatcgg acaggtaact gcttacgaaa
     6241 ccgccattga tgctcacttt gtccatcata aaagcggtgg ccttgcgcat gcaggttttc
     6301 acttcgtctt cggaacttgc ttttgtttcg gcccggaggc cggcactcgt cactaagaaa
     6361 agtgtcatac acaagataag cttgtgtcta aaattagttt tcaacaggta atgcagcata
     6421 atctttagaa taacgcaggt gttgttccac gtagttttcg ttgtgaagaa caatgatgtc
     6481 gataatttcg tctttgtcgt tgtaaaccgg tttatagagt ggatttacga atcctttgta
     6541 aggcgacagg ttcagtttgg cgtagcgatc cagaatttcc tggtgtaaca gagggtctac
     6601 ctgtacggca tagttttcga ccagggcttc ggcagccgtg taatcgcctt cggacttgat
     6661 gcgttgcact tcggccagca attcccccgt cagttgacgt aaggcttcgt aatcgtgtat
     6721 gaccaggtag gttttgttgt gacgcttttt taatttcatc actttatcct tgctgccctg
     6781 atcgagtaac caacgggcta tcagggccct gttgcgcata tgcgattctt cgatttgctt
     6841 gcccttctct attctgctca gttgggtcat caaaccgttc ataacgtatt cgtaatattc
     6901 tgctttatag gcttcctcat tgggaagcag gcccaattcc accagtttgg gatcggccag
     6961 aaagtacaag gcaaaaagat cggcccgggt ttcttctatc actgagccat aagacctgag
     7021 ggcgttagga tccacacccg gcaataactg gcctgagccg tggcccaggc attcgtgcag
     7081 gtcggtatgc aggttgccgg taataaagcc gtacttggcc gaggctttta cttcggcctc
     7141 ggaccacata aactcttcgt taaaaccgct gccttgcgac gattgaaaat acgcttcggt
     7201 gatgttttcg atggtgaccg atttggaacc gtaatcgcgg cgtatccagt cggcattggg
     7261 taaattgatg cctatggcgg tagtcggaaa ggcgtcgccc cccataatgg cattggtaat
     7321 tactttggcc gaaactcctt tgacttcttt ttttctgaat tcatcggcaa taggggcgtt
     7381 gtcttcgaac cattgggcgt tgtcgctgat gatctgggtg cgtttggagg cctcttcgtt
     7441 tttgaagttt acgatggctt cccaactgcc cttgatgccc agggggtcgc cgtagctttc
     7501 gatgaaaccg tttatgaaat caaccttcga ttccagatcc tgcacccaaa ggatgctgaa
     7561 ttgatcaaaa gtttccagat ctccgctgtg gtaataatcg atcaagcttt gaatgatttc
     7621 tttttgacgg ggattttcgg ctacttccaa ggccagttcc agccaatagg ttattttctc
     7681 caaggccggt gaatacagcc cgccgatctt ccagacctct tcgcgcagtt ggccctcctc
     7741 tttgaccagg cggctgttta agccgtagga aaccgggcag gggttttcgg gattgttgag
     7801 cacggcataa aaagcttcgg cctctttttg actaaggtcg cggtaataat tgcaggccga
     7861 actgactatc agatcttcgc cctgagcctg gtttactctc ttggcgtcga gccgggcatc
     7921 gaaaatcaga ggcagcaaac gggcagccag ttcatcggca tactgcgtgg tatttaatga
     7981 gtcctgcgtc tgcaacgggt tttgctcctg tgttagtcgc ccggattcca tggagggcag
     8041 tttttcggcg ggaactgccc taagggctgt tacaaaaaag tcttgcgaaa agccgggctc
     8101 aaacttatcg gtggaatagt gatgatgtat gccgttggca acccagaggc gtttaaggta
     8161 aacttcgaaa gcctgccagt cttcgccctg gcggctgata tcggcgtatt ggtaaatggc
     8221 ctccaggcag tgtctgatca ggagattgtg gcggtaattc tgatcgtaaa tgatatccct
     8281 cccccaaagg gctgcttcgg aaagataata aaccagtttt ttttgcctca gacttaggtc
     8341 ttcgaaaccg ggcacctgat agcggataat ttccatatcg gcaaagcgat ccactttgta
     8401 atcgaattct ttgaaggcag cttgtctgtt ggtgcagccg aaacacgaca aaataatact
     8461 cataagaaaa aagacttttt tcatatagcg tgaatttgtt taaactatgc gttctttcga
     8521 tttggccagc aggcaggcat cggccagtac cagggcggcc atggcttcca ctaccggcaa
     8581 aaccctgggc agtgcacaag gatcgtgccg tccctgaatt tgtattttca cggcagcacc
     8641 ttccttgtcg actgtttcct gaggtatacg aatgctggcg atgggcttga aggcggccct
     8701 gaaataaacc ggctgcccgt tggaaatgcc tccctgtatg cctccggaat gattactccg
     8761 ggtattgatg cgttcaccgt caaagtagaa ggcatcgttt tgctgagagc ctttttggct
     8821 gacatcgaaa ccggaaccgt actcaaaacc tttgcaggcc ggaatggaca ggatggccga
     8881 ggccaggcgg gcgtggagtt tgccgaaaac gggttctccc cagccgacgg gaagtcccct
     8941 gacaacacag ctgaccactc cgcctatact gtcttgttct tcctttaccc gggcaattaa
     9001 ggccatcata gcttcggctt tttcgcgatc ggggcaatgc agcggatttt cggcggccag
     9061 ctccgaagaa aacagcgtac tatcctgttg ttccagggcg atgggaccga tttggcttgt
     9121 aaaagcttct atgctaatgt tcatagtatt cagcagcatt ttggcaaagg ctccggccac
     9181 caccctggct atggtttcgc gggccgaaga acgtcctcct ccgcgatggt cgcgaatgcc
     9241 gtatttgctg tggtaggtgt aatcggcatg cgagggacgg aatattcctt tcaggtagtc
     9301 gtaatcctgc gaacgcatat cggtattcct gaccaaaaaa gcgatggggg ccccggtggt
     9361 tttgccttcg aaaataccgg aaatcagctc cacttcgtcc gcttcgttac ggttagtgct
     9421 cagggcagac tgcccggggc ggcggcgact cagttctttg cgtatcaatt cgagatccac
     9481 ggtcagaccg ggagggcaac cgtcgaggac tcctcctata ccccgtccgt gggattcacc
     9541 aaaagagctg aggcgaaaaa gttggccgaa tgtattcatt tcagtgtcag tcgttattaa
     9601 cacccgcaac cgcggcagct cccgtttgag ctctgtcctt cggagcagtc gtgtccgcag
     9661 ccggcgcagg aattcatcag ttgttccatt tcttcgtcgg tggcttcgcg aacttctagc
     9721 actttaccga cgaaatgcaa gtcttcgccg gccagagggt ggttgaagtc catgcgaaca
     9781 aattcgtcgg taatttcgag gactatgccg tcgagacgtt gaccttcggt ggtcatcata
     9841 ggaactattt tatccacggc aatgttttcc gaatcgaatt tgccgtctac ttcgaaaatc
     9901 gatttaggca aatccgctac ccgttcttcg tcgtattctc cataggctgt atcgcagggc
     9961 agtacaaaat cgaaggaatc accggcctgc agatttttta attttgattc gaaagattcc
    10021 aacatcgtac ccgttccaaa aataaaggta aggggctctt tttccgtggc tctttccatc
    10081 aactcgggag cctgcccgtc ttctccaccg acgtaaaggt cgtaggatgc tgcaacaaat
    10141 ttattatcag taattttcat aaattaggag aatgtaaaaa aataaacagt cgacaaatat
    10201 aaggatattt ttcgggcttc aaatcaattg taaacaactt acaagacctt tttgagcttc
    10261 ggtaaaattt gccggaacgg gatttcccat tatggaatat tcattctatt tttg
//
LOCUS       DAOSTD010000069        10208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_6392_length_10208_cov_14.859746, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000069 DAOSTD010000000
VERSION     DAOSTD010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10208
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6392_length_10208_cov_14.859746"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PLI37_05620"
     CDS             complement(<1..801)
                     /locus_tag="PLI37_05620"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HOQ58520.1"
                     /translation="MKKLLSVLAFSVIVFTFQAKQLSLNLKAGQIYQQEQKNQMDFQL
                     KIGGNEMLIHGNFDLVVEYLIESAQADAYEIQMSVKNITGNVGSDQFYFSLDAASSQN
                     NPLGAVLNLIVNKPIVYTMSKTGKILKMPDLSALSQDLKLSGDTTLYKFLEESMNANS
                     GGLMQSFYPDHEVAVGDSWTITSKIPNTGVEMTNTYTWKSSKGGKNTIEMESENFNLD
                     FSIPIRVDDGISIRIQMEAKTGGVFQLNKKTGWINASESLFEAKSKLSI"
     gene            complement(831..1778)
                     /gene="cysK"
                     /locus_tag="PLI37_05625"
     CDS             complement(831..1778)
                     /gene="cysK"
                     /locus_tag="PLI37_05625"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOQ58521.1"
                     /translation="MSHITKQLIDLIGNTPLLELSRYARYKGLKAELTAKLEYFNPGG
                     SVKDRVALAMIDQAESEGKLNPGGTIVEPTSGNTGIGLAVVSAVKGYRLILTMPETMS
                     LERRVTLKALGAEIVLTPGNGGMKAAIDKAEEIRTGIPGAITLDQFSNAANPAVHAES
                     TAREIWNDTSGRMDFFVGGVGTGGTVCGVGKGLKSFDPSIQIVAVEPQDSPVLSGGNP
                     GPHQIQGIGAGFVPKNYQATYVDRIMQVSYNEAVKTSRELARTEGLLVGISSGAAAFA
                     ATQLALQSENRRKRIVVLLPDGGERYLSTPLFDNDQLPL"
     gene            1970..2680
                     /locus_tag="PLI37_05630"
     CDS             1970..2680
                     /locus_tag="PLI37_05630"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOQ58522.1"
                     /translation="MENEVTYTMPRIGDKAPSFKAKTTQGDINFPEDFKGKWIILFSH
                     PADFTPVCTSEFMTFGSMAADFEALNCQLVGLSIDGLYSHIAWLRTIQEKIEYKGMKN
                     IEVKFPLIEDITMNVAKTYGMVQPGESSTQAVRAVFFIDPKGVVRTILYYPLSLGRNF
                     DEIKRILIGLQTADNFNVALPADWRPGEDVIVPTAGSCGVAKDRMENAGGDMTCYDWF
                     FCTKKLPKEEVEKKLGKK"
     gene            2768..3241
                     /gene="trxA"
                     /locus_tag="PLI37_05635"
     CDS             2768..3241
                     /gene="trxA"
                     /locus_tag="PLI37_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOQ58523.1"
                     /translation="MKKYLLVLAALLPLLSCNAQTSKSSSTNNENNKMEVINLTKADF
                     LAKVANYETNPSEWKYLGDKPAIIDFYADWCGPCRAVAPVLEELAKEYKGQIYVYKID
                     TEKEQELAAVFNIRSIPSILFVPMDGKPQMAQGALPKKTFIEAINAVLLNSASKN"
     gene            3316..3606
                     /locus_tag="PLI37_05640"
     CDS             3316..3606
                     /locus_tag="PLI37_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOQ58524.1"
                     /translation="MVNNNVKEGYLALFISVMCLVWILHLIGFLIVQPNKSAVLTFFG
                     VYSGTIKKNGFFWVNPFYGKRKLSLRARNLDVEPIKVNDKNGNPVINTGTLY"
     gene            3625..3780
                     /locus_tag="PLI37_05645"
     CDS             3625..3780
                     /locus_tag="PLI37_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008782442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HOQ58525.1"
                     /translation="MAQKKAFVLRVDEKMLKELEKWAADDFRSINGQIEWILNQALKK
                     QRRSKPE"
     gene            complement(3713..4318)
                     /locus_tag="PLI37_05650"
     CDS             complement(3713..4318)
                     /locus_tag="PLI37_05650"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOQ58526.1"
                     /translation="MSEKFYYEAPWARLIITIEDDQLSGIEFLSAETESSLNHRKHIF
                     NKGQTAQKVCKQLDEYFSGSRRVFDLPLRLRGTGFQKEVWEVLQQIPYGEVLSYGQVA
                     KTIGRPQAQRAVGMACNRNPVPIVVPCHRVLGTNGRLTGYAGGLDIKVRLLELEKKNK
                     VSFNNRFHAEPGFHNPAPSFIPALSVFAFSMPGLKSTLFAR"
     gene            complement(4315..5046)
                     /locus_tag="PLI37_05655"
     CDS             complement(4315..5046)
                     /locus_tag="PLI37_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOQ58527.1"
                     /translation="MTGLPNYTYRILARDLDFTGRASLMSLCRYIFNTASKDAENNGF
                     GTRELHNNNATWVISRMSFEMYATLREFDEFYINTWVNEISRLMTTRNLLLCDPEGKT
                     LGAAVTQWAMIDVDSRTPMDLRENMNYSKAARFDIPCPINKPVKIIPGKESDPVLERK
                     VVYSDLDFNRHVNSVKYLEWMLDMLPLENIINNGFSRLDMNYLREALYDDRLLLSYQQ
                     EDNHHLFDIKRKDETPVCRARIQWE"
     gene            complement(5059..6474)
                     /locus_tag="PLI37_05660"
     CDS             complement(5059..6474)
                     /locus_tag="PLI37_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ58528.1"
                     /translation="MDLRAKILAYFPYTPTSDQHKALDSCLNFFMFSGEMEVLLLKGY
                     AGTGKTALISAVVRFLTENRQPCVLMAPTGRAAKVFASYSGHAATTIHKKIYRQKEVD
                     GTYGRFDLNDNLHENTFFFVDEASMISNENFEGSFFGSGRLLDDLLRYVYSGKRCRLV
                     MMGDTAQLPPVGSAYSPALDRQNLESLGMQVAAVELTQVVRQEHQSLLLDNATALRRA
                     LAEQKVTEIPMLSTGKQTDVLSIRGEDLTDALQSAYDRCGIDECKVICRSNKQAVRYN
                     LGIRSRIFFKEEELSTGDLLLVAKNNYFWSQSYKEIPFIANGDLIEVRRVRKITELYG
                     FRFADLEVRFPETDWEMEVKVILDSLVSEAPALDQAAQQKLYEAVLLDYADVRSKKAL
                     YAKLRVDPWFNALQVKYGYAMTCHKAQGGQWKEIFLDQGWVTPEMLGAEYYRWLYTAL
                     TRAVERIYLVNFKDEYLINRD"
     gene            6594..7268
                     /locus_tag="PLI37_05665"
     CDS             6594..7268
                     /locus_tag="PLI37_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58529.1"
                     /translation="MRLFFRILLIIAVGLSVYLCVDSILRPIHFNREKDIRFNAVIGR
                     LIDIRKAQVEFRNYHGLYANDFDTLIGFVKHGYIPFVLKEGVLSDKQLEDGLTEEKAA
                     EIVYKKRTRDIKKWGLEGFRRDTFYANVLDTIFGKNYPIDSLRYVPFARNNEQFELRA
                     GDITTTSGLIVRVFEARTPYEVFMAGMDKQEVINMRLKAEKYDKYPGLMVGNIFEANN
                     NAGNWE"
     gene            7330..7971
                     /locus_tag="PLI37_05670"
     CDS             7330..7971
                     /locus_tag="PLI37_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOQ58530.1"
                     /translation="MEKPSELFSAEALETYCKNNTLQSARDTGSFILEYASNFLILPK
                     ELDPAYYFKFHFPEISGPTIQTLMLSDQKQQVVFELPQKHSELYQNYFPQTPLLCLPL
                     CFAEFVMGHSSGQTHCLFYLHQNLLMVFAARRGVLSYANRFDVVTDDAAAYFILSIRQ
                     VFETGGHCSLCTEKEPAGALQALLEPHFEQIDAYLLDRDLHLFISTLSGQIKL"
     gene            7968..8510
                     /locus_tag="PLI37_05675"
     CDS             7968..8510
                     /locus_tag="PLI37_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOQ58531.1"
                     /translation="MRIIRGTYKGRRFEIPHTLKARPTTDFAKEALFNILDNRLNYEE
                     LSVLDLFSGTGSIGLEFVSRGARTVYCIEQYAVHTRFIRSVVEKLGIQNLRLIQGDAY
                     QYIHKHPQVFDVIFADAPYNDPRLMELPDLIFEAGLLKPGGLLVVEHSKLQHFDMHPH
                     WEETRVYGSVRFSFFSQKKP"
     gene            8554..9615
                     /gene="aroB"
                     /locus_tag="PLI37_05680"
     CDS             8554..9615
                     /gene="aroB"
                     /locus_tag="PLI37_05680"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOQ58532.1"
                     /translation="MTKILTIRHQAELENSLNELLSDYEARQIFVLCDHNSKTHCLPL
                     IANFPCLKEAQHIIVPAGDEHKNMETLSAVWSSLSRKGGTRKSLLINLGGGMLSDLGG
                     FAAACFKRGIDFINIPTTLLACVDAALGGKTGINFEGLKNELGAFYPAKAVLIYPEFF
                     RSLDRDNLYSGYAEMLKHGLLSSHAHWQQVLSFDPVGASTRESAGLILDSMTVKEKIV
                     KEDPFENGPRKALNLGHTIGHAFESLSHKRNIALLHGHAVALGLVCELYLSNKLAGFP
                     EKELKACVNRVKQTYSAYPLQKSDYSELIGFMQHDKKNRDRHIRFCLLKDIGQTLPDA
                     TATPEQIEESLDYYCENLL"
     gene            complement(9741..9816)
                     /locus_tag="PLI37_05685"
     tRNA            complement(9741..9816)
                     /locus_tag="PLI37_05685"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9778..9780),aa:Pro,seq:ggg)
     gene            9989..>10208
                     /locus_tag="PLI37_05690"
     CDS             9989..>10208
                     /locus_tag="PLI37_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HOQ58533.1"
                     /translation="MQQALEDVLSRFSPILLEEMGKVKLMNRIDTKYIARVDRLIPLL
                     EEMTTGYYAQEINGKRIARYHTLYMDTPD"
BASE COUNT         2794 a         2428 c         2256 g         2730 t
ORIGIN      
        1 aatgctgagt ttagattttg cctcaaacag ggattccgaa gcgttaatcc agcctgtttt
       61 tttattcaac tgaaatactc ctcctgtttt ggcttccatc tgaatgcgta tgcttattcc
      121 atcgtcgact ctgattggta tgctaaaatc caaattaaag ttttcggact ccatttcgat
      181 ggtattctta ccgcctttgc tcgatttcca ggtataggtg ttggtcattt ctactccggt
      241 gttgggaatc ttgctggtga tggtccaact atcgccaaca gcaacttcat ggtcgggata
      301 gaaagattgc atcaaaccgc cggagtttgc attcattgat tcctccagaa acttatacaa
      361 agtggtatct cccgacaatt tcaaatcctg acttaaagcg ctcagatcgg gcattttgag
      421 aattttgccc gttttggaca tggtataaac aatcggtttg tttactataa ggttcaatac
      481 ggcgcccagg ggattgtttt gagaggaggc ggcatccaga ctgaaataga attgatcgct
      541 gccgacatta cccgtaatat tcttcacact catttgtatt tcgtaggcat cggcttgggc
      601 agattcaatc aaatattcaa caacaaggtc gaagtttccg tggatgagca tttcgtttcc
      661 tccgatcttc agttgaaaat ccatttgatt tttttgttcc tgttggtaaa tctgaccggc
      721 cttgagattg aggctcagct gcttagcctg gaaagtgaaa acgataacac tgaaagccaa
      781 tactgaaagt agttttttca tagtaagagt aataagatat taaaataagg tcataaagga
      841 agttgatcgt tgtcaaacaa gggagtggaa agatatcgtt ctcctccgtc gggcaaaaga
      901 accacaatac gtttgcgccg gttctcactt tgcagagcca attgagtagc agcaaaggct
      961 gcggcaccgg aggaaatacc taccaacaag ccttcggtac gggccagctc ccggctggtt
     1021 ttgactgcct cattataact tacctgcata atccggtcta catacgttgc ctggtaattt
     1081 ttgggaacaa agccggcccc tataccctgt atttggtgtg gtccgggatt gccgcccgat
     1141 aaaaccggtg aatcctgcgg ttcaacagct acaatctgaa tggaaggatc aaaagatttc
     1201 agacctttgc ccactccgca gacggttcca cccgtgccta ctccaccaac aaagaaatcc
     1261 atacgccccg aagtgtcatt ccatatttca cgtgccgtac tctcagcatg tacagccggg
     1321 ttagcggcat tgctgaattg atccaaagta atggctccgg gtatgccggt cctaatttct
     1381 tcggccttgt caatggcagc tttcataccg ccgtttccgg gggtcaggac aatttcggcc
     1441 cccagtgctt ttaaggtgac cctacgttcc agactcatgg tttcgggcat agttaaaata
     1501 agccggtaac ctttcacagc cgaaaccaca gccaaaccta taccggtatt gccactggtg
     1561 ggctccacta tggttccgcc cggattgagc ttaccctcgg attctgcctg atcaatcatg
     1621 gccagggcga cgcgatcctt tacgctaccc cccggattga aatattccaa cttggccgtc
     1681 aattccgctt tgaggccttt gtaccgtgca tagcggctta attcaagcaa gggagtattg
     1741 cctatgagat cgatcaattg tttggttata tgagacatct gatttttttg aatcctaata
     1801 gttcagttgc aaatgtagtg tttttttata aatcaatggt tttttgtatt tttttatgga
     1861 attgacgcta cctataacct catagatatt atctgttggt acgcagtgcc ggccttagta
     1921 tctttgtacc aacgaaaaat caattactaa tttaataaat tcaacaacta tggaaaatga
     1981 agtaacttac actatgccga gaatcggaga caaggctccg tctttcaaag ccaaaaccac
     2041 tcagggcgat atcaatttcc ccgaagattt caaaggaaaa tggatcatat tatttagcca
     2101 cccggccgat tttacaccgg tctgcacctc ggaatttatg acttttggca gtatggctgc
     2161 cgatttcgaa gcactcaact gccagctggt aggattgtcg atcgatggtt tgtatagcca
     2221 tattgcctgg ttgcgtacca tccaggaaaa aatcgaatac aagggaatga aaaatatcga
     2281 agttaaattc ccgttaatcg aagacatcac catgaacgta gccaagacct acggaatggt
     2341 gcaaccggga gaaagctcca cacaagccgt tagagcggtc ttctttatcg accccaaggg
     2401 tgtggttcgc actatattat attaccctct ctctctgggt cgcaattttg acgaaatcaa
     2461 acggatattg atcggattgc aaacggccga caatttcaac gtagcacttc ccgccgattg
     2521 gcgccccggt gaagacgtga ttgtacccac tgccggatct tgcggagtag caaaagaccg
     2581 aatggaaaac gccggcggcg atatgacctg ctacgactgg ttcttctgca ccaaaaaact
     2641 acccaaagaa gaagtagaaa aaaaactagg aaaaaagtaa attcatcagg ttaacaccag
     2701 gtttagtctt aaaagttcga gagggtgcct gaatacacag tcaccctcct ttttaaaaac
     2761 caaccatatg aaaaagtatc ttcttgtttt ggccgccctt ttgcctttgc tatcgtgcaa
     2821 tgcacagacc agcaagagtt catcaaccaa taacgaaaac aacaaaatgg aagtaataaa
     2881 tcttacaaaa gcagattttt tggccaaagt agccaactac gaaactaacc ccagtgaatg
     2941 gaagtatctc ggcgacaagc cggccatcat cgatttttac gccgattggt gcggtccctg
     3001 ccgggctgtg gcacctgtac tggaagaact ggccaaagag tacaaaggac agatatacgt
     3061 ctacaaaatt gacaccgaaa aagagcagga actggctgcc gtattcaata ttcgctccat
     3121 tccttcgatc ttgttcgtgc ctatggatgg taagccgcaa atggctcagg gtgcattacc
     3181 taaaaagacc tttatagaag ccatcaacgc tgttttgctc aacagcgcga gtaaaaacta
     3241 aatggtacca gtggatttat ccatctggcc ctgaacatcg tgtttcttgt gctggccgtt
     3301 gttttgaccg tcaatatggt taataacaat gttaaagagg gttacctggc gctttttatc
     3361 agtgttatgt gcctggtgtg gattttgcac ctgataggtt ttttgattgt acaacccaac
     3421 aaatcggccg tattgacctt ttttggtgtg tattcaggca ccattaaaaa gaacggattt
     3481 ttttgggtca acccttttta cggcaaaagg aaactttcgc tccgtgcccg aaatttggat
     3541 gttgagccca tcaaggtgaa cgacaaaaac ggcaacccgg tgattaatac cggcaccttg
     3601 tattaaaacc gtaacccgat agttatggct caaaaaaaag cctttgtact ccgagtcgac
     3661 gaaaagatgc tcaaagaatt ggagaaatgg gctgcggacg attttcgaag catcaacggg
     3721 caaatagagt ggattttaaa ccaggcattg aaaaagcaaa gacgctcaaa gccggaataa
     3781 acgacggagc cgggttatga aaacccggct ccgcgtgaaa acgattattg aacgagacct
     3841 tatttttctt ttcgagctcc agcaaacgaa ccttaatgtc gagtccgccg gcataaccgg
     3901 tcaagcggcc gttagtgccc aacacccggt gacagggcac gacaatggga acagggttgc
     3961 ggttgcaagc catgcccacg gcacgctgcg cctggggacg gcctatagtc ttggccacct
     4021 gcccgtaact aagcacctca ccgtaaggaa tttgttgcaa tacttcccaa acttcttttt
     4081 ggaaaccggt acccctgagc cggaggggca aatcgaagac ccggcgactt ccggaaaaat
     4141 actcatccag ttgtttgcag actttttgcg cagtttggcc cttgttgaat atatgctttc
     4201 gatggttaag tgagctttct gtttctgcgc taagaaactc aatgccgctc agttgatcgt
     4261 cctcaatggt aataatcagc ctggcccaag gtgcctcgta atagaatttt tcgctcattc
     4321 ccattgaatt ctggcccgac aaacaggtgt ttcgtcttta cgtttgatgt cgaacaaatg
     4381 atgattgtct tcctgctgat aggacaataa cagtctgtca tcgtataaag cttcgcgcaa
     4441 atagttcata tccagacggc tgaatccgtt attaatgatg ttctccaagg ggagcatatc
     4501 caacatccat tccagatact ttacactgtt cacatggcgg ttgaagtcca ggtcgctgta
     4561 cactacttta cgttccagca ccgggtcgga ctctttgccc ggaattattt tgaccggctt
     4621 gtttatgggg caaggtatgt cgaacctggc ggctttgctg taattcatat tttcgcgcaa
     4681 atccatgggt gtcctgctat caacgtcgat catggcccat tgcgttacag cggctcccag
     4741 cgttttgcct tcggggtcgc acaaaagcag gttacgcgta gtcatcagac gactgatttc
     4801 gttgacccag gtattgatat aaaattcatc gaattctctg agtgtggcgt acatctcgaa
     4861 agacatgcgt gaaatcaccc aggtagcgtt gttattgtgt aattcgcgcg ttccgaaacc
     4921 gttgttttcg gcatctttgc ttgctgtgtt aaatatataa cggcacagcg acataaggct
     4981 ggctcgaccg gtgaaatcca ggtcacgtgc caaaatcctg tacgtataat tgggaagtcc
     5041 tgtcatgcta ctattttttt aatccctatt aattaaatat tcgtctttga aattgaccag
     5101 gtaaattctt tccacagccc tggtcaaagc tgtatacaac cagcgatagt actcggctcc
     5161 caacatttcg ggggttaccc agccctgatc gagaaaaatt tccttccact gaccgccctg
     5221 agctttatgg caagtcatag cgtaaccgta ttttacctgt aaggcgttga accagggatc
     5281 tacacgcaat ttagcgtaca aagccttttt actgcgcaca tcggcgtaat cgagcaacac
     5341 ggcttcgtaa agcttttgct gagcggcttg atccaaggca ggagcttccg aaaccagact
     5401 atcgagtatc actttgactt ccatctccca gtcggtttcg gggaaacgca cttccagatc
     5461 ggcaaaacga aaaccgtaga gctcagtaat cttgcgcaca cgccttactt cgatcaaatc
     5521 gccgttagcg atgaaaggaa tttctttgta cgactgcgac caaaaataat tgtttttggc
     5581 caccaacaag agatcgccgg tggaaagttc ttcttctttg aaaaaaatgc gcgaacgtat
     5641 gcccaggtta tatcgtacgg cctgcttatt ggaacggcaa atcactttgc attcatctat
     5701 tccgcagcgg tcgtaagccg attgcagagc atctgtaagg tcttcacccc ggatgctgag
     5761 tacatctgtt tgcttgcctg tactcaacat cggaatttcc gttacttttt gttctgccaa
     5821 tgccctgcgc aaagcggtcg cattgtcgag caagagagac tggtgctcct gcctgaccac
     5881 ctgagtcaac tctacagcag ccacctgcat acccaacgac tccaggtttt gtcggtcaag
     5941 agccggactg taagccgaac ccaccggcgg caactgggca gtatctccca tcatcaccaa
     6001 acgacagcgc tttcccgaat acacatacct caataaatca tccagaagac gacccgaccc
     6061 aaagaagctg ccttcgaaat tctcgttgga tatcatcgag gcttcgtcta cgaaaaaaaa
     6121 agtattttcg tgcagattgt cgttcaaatc aaaccgcccg taggtgccgt cgacctcttt
     6181 ttgtcgataa atttttttat gaatggtggt ggcagcatgt ccgctgtaag aggcaaaaac
     6241 tttagccgct ctgccggtag gggccatcag cacgcaaggc tgcctgtttt cggtcagaaa
     6301 gcgcaccact gcactgatga gcgcggtctt acctgttcct gcataacctt tgagcaaaag
     6361 aacttccatc tcgcccgaaa acatgaaaaa atttaaacac gaatcgagcg ctttatgctg
     6421 gtcggaagta ggcgtgtagg gaaaatatgc aagaattttt gctcttaaat ccattaaaat
     6481 aaatttctgc ggtcattttt taggccgtaa aagtagcgat attcaaattc ttgtactaat
     6541 tttgtccaca ttaaaaatgt gaagttcagt acacaccatt aaattatcat cccatgagat
     6601 tattctttag aattttactt attattgccg tcggattgtc ggtttatctt tgcgtggaca
     6661 gtatcttacg ccctattcat tttaacaggg aaaaagatat tcgcttcaat gccgtaattg
     6721 gtcgtttgat agacatacgg aaagcccagg tggaattcag aaactatcac ggtctctacg
     6781 ccaacgattt cgatacgctg atcggctttg taaaacacgg ctatattcct tttgtattaa
     6841 aagaaggcgt gttgagcgat aaacaactcg aagacgggct gaccgaagaa aaagcagccg
     6901 aaattgtcta taaaaaacgc acaagagaca tcaagaaatg gggactggaa ggtttccgcc
     6961 gcgacacatt ctatgccaat gtgctggata ctatttttgg aaaaaattac cccatcgatt
     7021 ctctacgtta tgtccccttt gcccgcaaca acgagcaatt cgagctcagg gccggtgata
     7081 ttaccactac ttcgggtttg atagtccggg tgtttgaagc cagaacacct tacgaagtct
     7141 tcatggccgg tatggacaaa caagaagtca tcaatatgcg gctcaaggcc gaaaaatacg
     7201 acaaataccc cgggttgatg gtaggaaaca tatttgaggc aaacaacaat gccggtaact
     7261 gggagtaaca cgacaattgc atcgtttcca actatatcca tccggtatac gccggatgga
     7321 ttttgttttt tggaaaaacc ttcggaactc ttttccgcgg aggcattgga aacgtattgc
     7381 aaaaacaaca ctctgcaatc tgcccgggat accggctcat tcatcctgga atacgcttcc
     7441 aatttcctga ttttacccaa agagctggat cccgcttatt attttaaatt tcattttccg
     7501 gaaatcagcg ggccaaccat tcagacttta atgctgagtg atcaaaaaca acaggtggtt
     7561 tttgagttgc cgcaaaaaca cagcgaactt taccaaaatt acttcccgca aactcctcta
     7621 ctttgcctgc ctttgtgctt tgccgaattt gtcatgggtc acagctccgg gcaaactcat
     7681 tgtctgtttt acctccatca aaatctgttg atggtttttg ccgcacgcag aggggtgctc
     7741 agctacgcca accgttttga cgtcgttacg gatgatgcgg ccgcctattt cattttaagt
     7801 atacgacagg tcttcgaaac aggtggtcac tgcagtcttt gcaccgaaaa ggaaccggcc
     7861 ggggcgttgc aagccttact tgagcctcat tttgagcaaa tcgatgccta tcttctggac
     7921 agagatttgc acttgtttat atccacactg agcggacaaa ttaagctatg aggatcatta
     7981 gaggcacata caagggcagg cgtttcgaaa tacctcatac gctaaaggcc agaccgacga
     8041 ccgactttgc caaagaggct ttgttcaata tcctggacaa ccgcctgaac tacgaggagt
     8101 tgtcggtgct ggacttattt tcggggaccg gcagcatcgg actggaattt gtttctcgcg
     8161 gcgcccgcac agtttactgc attgaacaat acgcagttca tacgaggttt atacgctcgg
     8221 ttgtcgaaaa acttgggata caaaatctca gattgatcca gggagatgct taccagtaca
     8281 ttcacaagca tccgcaggta tttgatgtga tctttgccga tgctccgtac aacgatccca
     8341 ggcttatgga attgcctgat ctgatttttg aggccggcct actcaagccg ggaggtctgc
     8401 ttgttgtgga acactcaaaa ttgcagcatt tcgacatgca tccccattgg gaagaaacta
     8461 gagtgtacgg cagcgtgcgt tttagtttct tcagtcaaaa aaaaccatag ccgaaacttt
     8521 ttataggttt gcggccgtaa aacagccttc tgcatgacaa aaatactgac catccgccat
     8581 caagccgaac tggaaaacag cctgaatgaa ttactctcgg attacgaagc ccgccagatt
     8641 tttgttttat gcgaccacaa cagtaaaaca cattgcctgc ctctgatcgc aaattttccc
     8701 tgccttaaag aggctcaaca cattatcgta ccggccggtg acgaacataa aaacatggag
     8761 accttgtctg ctgtctggtc ttcactaagc cgtaaaggcg gaacccgcaa atcgctgctc
     8821 atcaacctgg gcggaggtat gctgagcgat ctgggcggct ttgcggctgc gtgtttcaaa
     8881 agaggcatcg acttcatcaa catacccacc accctattag cctgtgtaga cgcagctctg
     8941 ggaggcaaaa ccggcatcaa cttcgagggc ctgaaaaatg aacttggcgc cttttatccg
     9001 gccaaggccg tattgattta cccggaattc ttccgcagcc tcgaccggga caatttgtat
     9061 tcgggctatg cagaaatgct caaacacggg ctcttgtcgt cccatgctca ttggcagcag
     9121 gtgttgagct ttgatccggt cggggcctct acccgggaaa gtgccgggct tattctggat
     9181 tccatgacgg ttaaagaaaa aattgtcaaa gaggatcctt tcgagaatgg cccgcgcaaa
     9241 gccctgaacc tgggacatac catagggcat gcattcgaga gtctttccca taaacgaaac
     9301 atagccttgc ttcatggcca cgctgtggcc ctgggtttag tctgcgagct ttatctttca
     9361 aataagctgg ccggtttccc cgaaaaagaa ctgaaagcat gtgtgaatcg cgtaaaacaa
     9421 acctattccg cctacccact gcaaaagtct gattattcgg aactaatcgg gtttatgcaa
     9481 cacgacaaaa agaaccgcga tcgtcatatt cgtttctgcc tgttgaaaga tatcggccaa
     9541 accctgccag acgcaacagc tactccggaa caaatcgaag aaagtctgga ttattactgt
     9601 gaaaacttac tataaaagga tcaaaacacg ggatcaaaac acgggatcaa accctgcggc
     9661 gcattgattc catttctttt taaaaccaaa agaaataaaa agcgttccgg gattcattga
     9721 ttcccggaac gctttttcag tcggggtgac tagattcgaa ctagcgacca ctcgcccccc
     9781 agacgagtac tctaaaccgg actgagctac accccgaaag cggccgcaaa tgtaagacga
     9841 atttttggat tgaacaaact ccgccccctt tttatttatg acaatttagt cgaatattat
     9901 accaaaatca actcataatg ttgatagtac ttggtgatac cgtgcatttt agttactttt
     9961 gtagattaaa ttaataccgt aaaaacaaat gcaacaggcg cttgaggatg tattaagtcg
    10021 gttttcccct attttgctcg aagaaatggg aaaggtaaag ctaatgaacc gtatcgacac
    10081 caaatatatt gcacgtgtcg accggttgat tcctttgctt gaagaaatga cgacaggata
    10141 ttatgctcag gagatcaacg gcaaacgcat tgcacgatac cacaccctgt acatggatac
    10201 acccgatg
//
LOCUS       DAOSTD010000070         9763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_6721_length_9763_cov_11.456840, whole genome shotgun sequence.
ACCESSION   DAOSTD010000070 DAOSTD010000000
VERSION     DAOSTD010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6721_length_9763_cov_11.456840"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PLI37_05695"
     CDS             <1..604
                     /locus_tag="PLI37_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOQ58534.1"
                     /translation="ELAETQGKVAWTRVSVGFLAGGFFLWLLDSLLPHLHPGSKTPEG
                     LPTGWRRSTMLLLALTLHNIPEGMAVGLSFGFSGAVEQGFSLASAFALAIGIGLQNIP
                     EGMAVALPLKQATGSRFKAFLYGSLSGVVEPIAGILVVFLAGSAFGFMPWLLAFAAGA
                     MIYVVVEELIPSFHEGEHSNMGTIGVMIGFALMMILDISL"
     gene            616..4374
                     /locus_tag="PLI37_05700"
     CDS             616..4374
                     /locus_tag="PLI37_05700"
                     /inference="COORDINATES: protein motif:HMM:NF025965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4450 domain-containing protein"
                     /protein_id="HOQ58535.1"
                     /translation="MKKFSAFFLLFLFFCLSSCVQNDRGSSENRELRYLPQGSDFVFR
                     ADSARKFNRALYGNNQAFRLETGDVPEFGFYSSAMLGNLRFALVKGEQKRWLHEAKTI
                     EYRYRPGLSIYSIQDPILGRGSLELHALARYDNEGSLFRFEFSRPDPELQLIAVYGGA
                     GGKRFSRDGDMGADPHDAFELKTAYCRYNRYHIRASAFQLWCGASLDISGLTEAALND
                     PVNKLRTFYGIFPEEAELQLGNAEKPNDLDHLLKAEPQQDQPLLLARMPLNKKQVFFM
                     TLGVGLLPEAPENEQTSPTEKLSVHDPSSSTQPGQKELEIAYNQALESAAGIAGRLKI
                     SSPDPYINAMGGALAMAADAIWESPSYLHGSIGWRSRLAGWRGAYTGDVLGWHDRARE
                     HFRAYGASQMTLPPDKGVIMDTALNLARSAKVAGSEMYSSGYIARYPNNPGTMNHYDM
                     NLCYIDELLWHFRWTGDLEFVREMWPVLTRHLAWEKRCFDPDDDGLYDAYCCIWASDG
                     LQYNGGAVTHSTAYNYRANLMAAELAELLGLDARPFHNEADKIREAVGRVLWLSQKGY
                     WAEFRDAFGLQRLHEQAAVWTIYHAIDAELHDPFTGWQALNYIEHEIPHIPLKINTKG
                     LSKNDMPEADLAVISTSNWQPYVWSVNNVAFSEIMHTALACFQGGRPEMGFKLMKSAI
                     MDGMYLGGSPGNVGQVSYYDAARGEVYRDFADVVGVYSRAMVQGLFGIEPDLLHNKVY
                     LRPGFPAQWDKASIEIPDLSYRFERKGNTSRWQIHSGFGRDLNYVLELPLRYDQTPLV
                     RINGTELAEVNIISSVEKPLLQIHCPSGRQFDIEIIEQGNSREVWNFPSVLAIGEELS
                     LPLPGKAEMLDIYDPQSVLQQVQIKDNQFQAVVSGLEGPRSFFVLLKQGDISWWQVLA
                     PALKPGLEIIGEEGNREEKQLSFRIRNNYLSRNLQAELFLNGEKIKTLKLSAQSLSDP
                     IHNTKAARMGSNLVEIRQKGTTLAQARVINWNLPPASPEQQETIDLNSYYNDKLNDIF
                     RFGKYLSPRSPFTTLQIPTQGIGDWCSPMHIVNIDDKGVRKAAGVNNLINTRLGLNFA
                     SPGDSLRNNILFVSHWDNYPTLAHLPLQGKAQHAYLLMAGSTNHMQYGLPNGKLCFYY
                     SDGSCDSLLLINPENWPAIEQDAFDDGLAFKLKAPRPWRLLLTTGELSKEPGKLLKLR
                     GAAGRYIPGGAATILDLTLNPGKELSYLEFEALSNEVVLGLMSLTLIR"
     gene            4501..5586
                     /locus_tag="PLI37_05705"
     CDS             4501..5586
                     /locus_tag="PLI37_05705"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOQ58536.1"
                     /translation="MISTALLIFFTAFIVSCIAMPNIIKISVKKLLFDAPTEERKIHK
                     KNISNLGGIGIFIGFFFSILLYRIICDSLELSSLAAAAVLLFFVALKDDLIPSSPISR
                     LFYQVLIASIIVFIGQVHFDHIPIFEAGTTMRRIVNILFSLFFVVGIINAMNFIDGID
                     GLAASLSVFLSLIFGYIFYQYGQLNYSYTSLALGGAVLGFLIFNFAPARIFMGDIGSM
                     LIGVFMAIFGIQLANMDTLPLGLLQIRYPGTIMFALLIVPIMDLITVVGIRLYLKKSP
                     FEADKRHLHHRLLVLGLSHPAICFSLLLFNILVLAVAFLLQKTDSSLWAALAITLLAA
                     TFELLVLWLYSKKKKLDDKELDIVNHK"
     gene            5626..6525
                     /locus_tag="PLI37_05710"
     CDS             5626..6525
                     /locus_tag="PLI37_05710"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004326069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOQ58537.1"
                     /translation="MQKIKAAIVGYGNIGKYLLEALQSSPDFELTGIVRRDPQGVQPV
                     ELRPYRVVASVKELPQTEVAILCTPSRSTQKYAAEMLSLGIHTVDSFDVHSDIWDVKQ
                     KLDKIAKENKRVSVISAGWDPGSDSVVRALMEASVPKGITYTNFGPGMSMGHTVAVKA
                     VPGVKAALSMTIPLGSGVHRRMVYIELEKGYDFNKVASAIKADAYFVHDETHVVQVED
                     VNALLDMGHGVHLVRKGVSGKTQNQQLEFSMRINNPAFTAQLLVAAARASIKQAPGCY
                     TMIELPMIDLLPGNREDLIRRLV"
     gene            complement(6618..>7457)
                     /locus_tag="PLI37_05715"
     CDS             complement(6618..>7457)
                     /locus_tag="PLI37_05715"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOQ58538.1"
                     /translation="LFDLYAHTGEPRFKMAIDSIMDLQLRQQPRTHQGGFWHKKIYPY
                     QMWLDGLYMAGPFIARYAREFDEPEWTDEVIHQLILVGRQTYDSKTGLFYHAWNSNRE
                     QAWANPQTGCSPNFWGRSIGWYAMALVDVLDYIPEEHPRRGEVIKLLKKLSAGMLRWQ
                     DGRTGLWYQVVDQGQREGNYLESSVSAMMMYTYAKGYNKGYLGRKYLKAANEVLDGLI
                     AHRIVANEDATISLTYCCAVSGLGGNPYRDGSFEYYVNERIRNNDGKATGPFILGCLE
                     LGR"
     assembly_gap    7458..7557
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7558..9104)
                     /gene="ettA"
                     /locus_tag="PLI37_05720"
     CDS             complement(<7558..9104)
                     /gene="ettA"
                     /locus_tag="PLI37_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOQ58539.1"
                     /translation="MSDDKKIIFSMVGVSKIFPPHKQVLKDIYLSFFYGAKIGIIGLN
                     GSGKSTLMKIIAGIEKEYQGNVVFSPGYSVGYLEQEPKLDNDKTVKEIVMEGVQEVMD
                     VLKEYEDINQLFALPEYYEDADKMQKLFDRQAELQNKIDATDAWNIDNKLERAMDALR
                     CPPDDQIVATLSGGERRRVALCRLLLQQPDVLLLDEPTNHLDAESIDWLEQHLQQYPG
                     TVIAVTHDRYFLDHVAGWILELDRGEGIPWKGNYSSWLEQKTQRMAMEEKQASKRRRT
                     LERELEWVRMAPKARHAKGKARLNSYDKLLNEDQKQREERLELFIPNGPRLGNKVIEA
                     VDVAKAYGDKCLFEELNFSLPPNGIVGVIGPNGAGKTTLFKLIMGLEKADKGSFEVGE
                     TVRIGYVDQSHEAIKADQSVYEIVSGGQEFIRVGGREINARAYLSRFNFTGADQEKKA
                     GVLSGGERNRLHLALNLKSEANVLLLDEPTNDIDVNTLRALEEGLENFAGCAVVISHD
                     RWFLDRI"
     gene            9271..9762
                     /locus_tag="PLI37_05725"
     CDS             9271..9762
                     /locus_tag="PLI37_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOQ58540.1"
                     /translation="MIGQEQVYTRLKELNIDFHYLEHPPAHSIEIGRPYWEHLPGIHC
                     KNLFFRNHKGNRHYLVVFHCEQNLAIRDLEQRLRQGKLTFASEKRMLKYLGLQPGSVS
                     PFGLINDLEHHVYVFLDKNLRNFEFLSFHPNDNRASLTISSRDFVRYMESSGNEFEWL
                     ELY"
BASE COUNT         2390 a         2537 c         2263 g         2473 t
ORIGIN      
        1 cgaactggcc gaaacccagg gcaaagtagc ctggaccaga gtttctgtag gatttttggc
       61 gggcggcttc tttctctggc tgctcgactc tctgctacct cacctccacc ccggcagcaa
      121 aactccggaa ggcttgccta caggttggcg ccgaagcact atgcttttgt tggcacttac
      181 cttgcacaat atccccgaag gaatggcagt cggtttgagc tttggttttt cgggggcggt
      241 ggaacaaggt ttttcgctgg cctcggcttt tgcacttgcc attggaatcg gcttgcagaa
      301 tatccccgaa ggaatggcag ttgccctgcc cttgaaacaa gccacaggct ccaggtttaa
      361 agcctttttg tatggttcac tctcgggcgt agttgaaccc attgccggta ttctggttgt
      421 tttcctggcc ggctcagctt ttggttttat gccctggttg cttgcctttg cggctggagc
      481 catgatctac gtggtggttg aagaacttat tccatcgttt catgagggcg agcattccaa
      541 tatgggaacg ataggtgtga tgatcggttt tgccctgatg atgattctcg atataagctt
      601 gtaaaatatc cgctgatgaa aaaattttct gcttttttcc ttctcttttt gtttttctgt
      661 ctgagctcct gcgttcaaaa cgatcgcggc tcatcggaga acagggagct caggtattta
      721 ccccaaggct ccgattttgt gttccgtgcc gattctgcgc gtaaattcaa tcgcgcgctt
      781 tacggtaaca atcaagcatt caggctcgaa acaggcgacg ttcccgaatt tggcttttac
      841 agctctgcca tgttgggcaa tctgcgcttc gctctggtca aaggcgaaca aaaacgttgg
      901 ctgcacgaag ccaaaacaat agaataccgc taccgtccag gtctgagcat ttacagcata
      961 caagacccca ttttgggccg tggcagcctc gaattacatg cccttgcccg ttacgacaac
     1021 gaaggatctc tctttcgttt tgaattcagc cggccggatc ctgaactgca attaatcgcc
     1081 gtttatggcg gagccggcgg aaaacgcttt agtcgcgacg gcgatatggg cgccgatcca
     1141 cacgatgctt ttgaactgaa aacagcctat tgccggtata accgctacca tatacgggct
     1201 tccgcatttc aactttggtg tggagcaagc ctggatatat caggtttgac cgaagcagct
     1261 ttgaacgacc cggtcaacaa actcaggact ttttacggaa tttttcccga agaagcggaa
     1321 cttcaactcg ggaatgcaga aaaacccaat gatctggatc atctgctcaa ggccgaacca
     1381 caacaagacc aaccactgct actggcccgg atgcccctga acaaaaaaca agtctttttt
     1441 atgaccctgg gcgtgggttt gctcccggaa gcacctgaaa acgaacaaac ttctccaacc
     1501 gaaaagcttt ctgttcacga cccgtccagt tcaacacaac ccggacaaaa agagcttgaa
     1561 atcgcatata atcaggctct tgaatccgca gcgggtatcg ccggcagact aaagatcagc
     1621 agtccggatc cttacatcaa tgctatgggc ggagccttag ccatggcagc cgacgccatc
     1681 tgggaaagcc cctcctacct gcatggttcc ataggctggc gcagccgttt agcaggctgg
     1741 agaggcgcct acaccggcga tgtgctgggc tggcacgaca gagcccggga gcatttccgc
     1801 gcttacggag cctcacaaat gactcttcct cccgacaaag gagttattat ggacaccgcg
     1861 ctcaacctgg cccgctcggc caaagtggcc ggcagcgaaa tgtacagttc aggctacata
     1921 gcccgttatc ccaacaatcc gggcaccatg aaccactacg acatgaacct ctgctacata
     1981 gacgaattgc tctggcattt tcgctggacc ggcgacctgg aatttgtacg cgaaatgtgg
     2041 ccggtattga cccgtcacct ggcttgggaa aaacgctgct tcgaccccga cgacgacgga
     2101 ctttacgacg cctactgctg catctgggcc agcgatggtc tacagtacaa cggaggggct
     2161 gtgactcatt caaccgccta caattaccgg gcaaatctaa tggcggccga attggctgaa
     2221 ctcctgggtc tggatgcacg gccttttcat aatgaagccg ataaaatccg agaggctgtc
     2281 ggtagagtat tgtggttatc tcaaaaaggt tattgggccg aattccgcga tgcctttggt
     2341 ttacaaagac tgcacgaaca ggccgccgtc tggaccatct accatgccat cgatgcggaa
     2401 ttgcacgatc cttttacggg ctggcaggcc ttgaattaca ttgaacacga aattcctcat
     2461 atccccctaa aaattaatac caaagggctt tctaaaaatg atatgcctga agcagacctg
     2521 gccgtgattt ccacctccaa ctggcaacct tacgtctggt cggtcaacaa cgtagccttt
     2581 tccgaaataa tgcataccgc tttggcctgt ttccagggag gcaggcctga aatgggcttc
     2641 aaactgatga aatcggccat catggacggt atgtatctgg gcggtagccc cggcaatgta
     2701 ggacaagtta gttactacga tgctgcccgg ggcgaggtat atcgcgattt tgccgatgtg
     2761 gtgggcgttt attcacgcgc aatggttcaa gggctgttcg gtatcgaacc cgatcttttg
     2821 cacaacaaag tgtatctgcg tcccggcttc ccggctcaat gggacaaggc ttcgatagaa
     2881 attccggacc ttagttaccg tttcgaacgc aaaggaaata ccagccgctg gcaaatacat
     2941 tccggcttcg gtcgtgacct gaattacgta ttggaactgc ctctgcgcta cgaccaaaca
     3001 cccttggtcc ggattaacgg aacagagctt gccgaagtaa acatcatcag ctcggtcgaa
     3061 aaaccgcttt tgcaaattca ttgcccttcg ggccggcaat tcgacattga aataatcgag
     3121 caggggaaca gcagggaagt atggaatttc ccgtctgtgc tggccattgg cgaagagttg
     3181 tccctgccgc tgcccggcaa agccgaaatg ctcgatatct acgatcctca gtcggtgctg
     3241 caacaagtac aaatcaaaga caatcaattc caggcagttg tgagcggact cgaaggcccc
     3301 cgcagcttct ttgttttact taaacagggt gatatcagtt ggtggcaggt tctggcaccg
     3361 gcacttaagc ccggcttgga aattatcggc gaggaaggta atcgggagga aaaacaactt
     3421 tctttccgca tccgcaacaa ttacctaagt cgaaacttgc aggcagagct cttcctgaac
     3481 ggagaaaaaa tcaaaactct caagctgtca gcacaaagcc tttcggatcc cattcacaac
     3541 accaaagctg cccgcatggg cagtaacctg gtcgaaatca gacaaaaagg aacaacactt
     3601 gcgcaggcac gggtaatcaa ctggaatctg cctccggcat ctcccgaaca acaggaaacc
     3661 atcgacttga actcttatta caacgacaaa ctcaacgata ttttccgttt cggcaaatac
     3721 ctcagtccgc gctctccttt taccacactc caaatcccca cccagggtat cggcgattgg
     3781 tgcagcccta tgcacatagt gaatatcgac gacaagggtg tacgaaaagc agccggagta
     3841 aataatctga tcaacaccag gttgggactg aattttgcca gccccggcga cagcctccgg
     3901 aacaacattc tttttgtttc gcattgggac aattacccca ccctggccca tttgccatta
     3961 cagggtaaag cgcagcatgc ctatctgctt atggccggtt ccaccaacca tatgcaatac
     4021 ggcctgccca acggtaaact ctgtttttat tatagcgacg gaagctgcga cagtttgttg
     4081 ttgatcaatc ccgaaaactg gccggccatc gaacaggatg ccttcgatga cggactggcc
     4141 ttcaagctca aagctccccg tccctggcgt ctgctgctga caacgggaga gcttagtaaa
     4201 gaacccggca agcttttgaa actaagagga gcggccggcc ggtatattcc cggaggcgct
     4261 gccaccatac tggatctaac gctgaatccc ggcaaggaat tgagttacct tgaattcgaa
     4321 gctttgagta atgaagtggt tttgggtctt atgtccctga ccctgataag ataattataa
     4381 cagctattat gctctgtata aatattataa cattatacga aaaaaaagct aaaaaaaatt
     4441 ctctttcaac gacaataaag caggactttt tgagttatta gtataaacta tgatttgcgt
     4501 atgatatcga cagctctact catttttttt acggctttta tcgtttcctg catcgccatg
     4561 ccgaacatca tcaagatttc ggtcaaaaag ctcctgttcg atgctcccac cgaagaacga
     4621 aaaattcata aaaagaatat ctccaacttg ggcggtatag gtattttcat cggattcttt
     4681 ttcagtattt tactctacag aatcatatgc gactcattag aactcagcag tttagcggca
     4741 gcggccgtgt tgttgttttt tgtagccctc aaagacgacc ttatcccttc ctctcccatc
     4801 agccggctgt tttatcaagt actgattgct tcgatcatag tttttatcgg tcaggtacac
     4861 ttcgaccaca ttcccatttt tgaagccggc acaactatgc gccgtatagt aaatattttg
     4921 ttctcactgt tctttgtggt gggcatcatc aacgctatga actttattga cggcatagac
     4981 gggctcgctg ccagcctgag cgtgttcctg agcctgattt tcggttatat cttttatcag
     5041 tacggtcaat tgaattattc ctatacttcg ttggctctgg gtggagccgt tctgggtttc
     5101 ctgatcttta attttgcccc ggcccgcatt tttatgggcg atatcggctc catgctgatt
     5161 ggggtattta tggccatttt cgggattcaa ctggccaata tggataccct ccccttgggt
     5221 cttttgcaaa tcaggtatcc ggggaccatt atgtttgccc tactcatcgt gcccatcatg
     5281 gacctgatca cggtagtggg tataagactt tacctgaaaa aatctccttt cgaggcagat
     5341 aaacgccacc tgcaccatcg gctattggtg ctcggtctga gtcacccggc tatttgcttt
     5401 agcctacttt tattcaatat tcttgtgctg gctgtggctt ttttgctgca aaaaaccgat
     5461 agttctcttt gggcagccct ggctatcacg ttactggctg caaccttcga gttgctggtt
     5521 ttgtggttat attccaagaa gaagaaactc gacgataaag agctcgatat cgtcaatcac
     5581 aaataagagg aaagcaaatt tgctcccttc aaaatccctc attctatgca aaaaattaaa
     5641 gccgccattg tcggatacgg aaatattggc aaatacctgc tggaagctct gcaaagctct
     5701 cccgactttg aattaaccgg cattgttcgc cgtgaccctc aaggagttca gcctgttgaa
     5761 ttacgtccct accgggtagt ggcctctgtc aaagaactcc cccaaaccga ggtggctatt
     5821 ttgtgtactc ccagccgcag cactcaaaaa tacgctgcag aaatgctcag cctgggcatc
     5881 cataccgtcg acagttttga tgttcacagt gacatttggg atgtgaaaca aaaactcgat
     5941 aaaattgcca aagaaaacaa acgggtttcg gtgatctcgg ccggctggga tcccggcagc
     6001 gactctgtgg ttcgtgctct gatggaagct tctgttccca aaggcatcac ctacaccaac
     6061 ttcggccccg gcatgagcat gggacatacc gtcgccgtca aagccgtgcc cggcgttaaa
     6121 gcagctcttt cgatgaccat ccctttaggc tccggcgtac accggcgcat ggtctacatc
     6181 gaactggaaa aaggctatga ttttaacaag gtagcttcgg ccatcaaagc cgacgcctat
     6241 tttgtacacg atgaaaccca cgtggtgcag gtcgaagacg tcaatgccct actcgatatg
     6301 ggtcacggag ttcatttggt ccgcaaaggc gtttcgggca aaacccaaaa tcagcagttg
     6361 gaattcagca tgcgtatcaa caatccggcc ttcaccgccc aactcttggt ggccgcagcc
     6421 agggcaagca taaaacaagc tccgggttgt tataccatga tagaattgcc aatgatcgat
     6481 ttactgcccg gcaacagaga agacttgatt cgcaggttag tctgaagcaa atttacagcc
     6541 gcccctacaa aaacaaaact cagacaccca aataaccgac aggaaggcaa aaccggcagg
     6601 acggaaagtc gtaagcttta acgtcccagt tccagacagc ccaaaataaa gggcccggta
     6661 gctttgccgt cgttgttccg tattctttcg ttgacgtaat actcaaaact gccgtcccga
     6721 tagggattac cccctaggcc cgaaacagcg caacaataag taagcgaaat ggtagcgtcc
     6781 tcgttggcca ctatgcggtg agcaatcagt ccgtccaaca cctcgttggc ggccttaagg
     6841 tattttctgc ccagatagcc cttattatag cctttggcgt aggtgtacat catcatggca
     6901 gaaaccgaag actccaggta attgccttcg cgctgtccct gatccaccac ctgataccaa
     6961 agtcccgtac gtccgtcctg ccagcgcagc atgccggcgg agagtttttt gagcaatttt
     7021 atcacttcgc ctctgcgggg atgttcttcg ggaatataat ccagcacatc caccaaggcc
     7081 atggcatacc aaccgatgct gcgtccccag aaattgggcg aacaacctgt ctgcggattt
     7141 gcccaggctt gttcgcggtt ggaattccag gcatgataaa ataagccggt tttggagtcg
     7201 taggtttgtc tgcccaccaa aatcaattga tggatcacct cgtctgtcca ttcgggctca
     7261 tcaaattccc tggcgtaacg ggcaataaaa gggccggcca tgtaaagtcc gtcgagccac
     7321 atctgatagg ggtagatttt cttatgccag aagccgccct ggtgagtacg gggctgttgg
     7381 cgcaactgca gatccataat gctgtctatg gccattttga aacgaggttc cccggtatgg
     7441 gcataaaggt caaaaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncaa
     7561 atacggtcca ggaaccagcg gtcgtggctg ataaccaccg cacagccggc aaaattttcc
     7621 aggccttctt ccagagccct cagggtattg acatcgatgt cgttggtggg ttcgtcgagc
     7681 aataacacat tggcttccga cttgagattc aaggccagat gcagtcgatt gcgttcaccg
     7741 cccgacagca caccggcttt tttctcctga tcggctcctg taaagttgaa gcgcgacaaa
     7801 taagccctcg cattgatttc cctgccacca acccgaataa attcctgacc gccggacact
     7861 atttcgtaca cgctttgatc ggccttgatg gcttcgtggc tttgatccac gtagccgatc
     7921 cgtactgtct ctcctacttc gaaactcccc ttgtcggctt tttccagacc catgatcaat
     7981 ttaaacaggg tggttttgcc ggcgccgttg ggacctataa caccaacaat accgttggga
     8041 ggtaaactga aattcaattc ttcaaagagg catttatccc cataggcttt ggccacatcc
     8101 accgcctcaa ttaccttgtt gcccaggcgg ggaccgttgg gaataaacaa ttccagacgt
     8161 tcttcacgtt gtttctggtc ttcattgagc agtttatcgt aagaattcaa acgggcctta
     8221 cctttggcgt gacgggcttt gggagccata cgcacccatt ccagctcgcg ttccagggtg
     8281 cgccggcgtt tgctcgcttg cttttcttcc attgccatgc gctgggtctt ttgttccagc
     8341 caggagctgt aattgccctt ccagggaatg ccctcgcctc tgtccagttc cagtatccat
     8401 ccggccacat gatccaaaaa atagcggtcg tgagtgacgg cgattaccgt gccggggtat
     8461 tgctgcaaat gctgttccaa ccagtcgatc gattcggcat ccaggtggtt ggtgggttcg
     8521 tcgagcaaca acacatcggg ttgttgcaac aacaaacggc agagcgctac ccggcggcgt
     8581 tcaccgcccg aaagtgtggc aacaatttga tcgtcgggag gacaacgcag ggcgtccatg
     8641 gcccgttcca gtttattgtc gatgttccag gcatcggtgg catcgatctt gttttgtaat
     8701 tcggcctgac gatcgaaaag cttttgcatc ttgtcggcat cctcgtaata ttcgggcaag
     8761 gcaaacagct gattgatgtc ctcgtattcc tttaatacgt ccatcacttc ctgcaccccc
     8821 tccatcacta tttctttgac ggttttgtca ttatccagct taggttcctg ttccagataa
     8881 ccaaccgagt aaccgggcga aaacaccaca ttgccctggt attccttttc gataccggca
     8941 atgatcttca ttagggtaga ttttccggac ccgttaagac ctatgatgcc gatcttagcc
     9001 ccgtaaaaaa atgaaagata aatgtctttt agtacttgct tgtgcggtgg gaagatttta
     9061 ctcacaccta ccatcgagaa aattattttt ttgtcgtcac tcatgtttct gctttatgaa
     9121 agggctgaca aagataataa aaatcctaac tttgtaccgt tatcgcgatt atacgcttat
     9181 ttaagcctaa attaagcaag gacggcgtga ctgccttctt tcaaaattcg cgattatacg
     9241 cttattcaag cccaaatctt cattagcact atgattggac aagagcaggt ttacaccaga
     9301 ctcaaagaac tgaacatcga ttttcattat ctggagcatc cgcccgcaca cagcatcgaa
     9361 atcggcagac cctactggga acatctgccg ggcattcatt gtaaaaatct ttttttcaga
     9421 aatcacaaag gcaaccggca ttacctggtg gtgtttcatt gcgagcaaaa cctggccatc
     9481 cgcgatctgg aacaaaggtt acggcaaggc aagctgactt ttgcctccga aaagcgcatg
     9541 ctcaagtact tgggcctgca gcccggttcg gtgagccctt tcggactgat caacgacctc
     9601 gaacaccacg tctacgtatt tctcgataaa aacctccgga acttcgaatt cctgtctttt
     9661 caccccaacg acaaccgggc ctctcttacc atcagcagcc gcgattttgt ccgctacatg
     9721 gaaagcagcg gcaatgagtt tgagtggctg gagttgtatt gat
//
LOCUS       DAOSTD010000071         8959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_7416_length_8959_cov_14.956649, whole genome shotgun sequence.
ACCESSION   DAOSTD010000071 DAOSTD010000000
VERSION     DAOSTD010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7416_length_8959_cov_14.956649"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..1641)
                     /locus_tag="PLI37_05730"
     CDS             complement(262..1641)
                     /locus_tag="PLI37_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOQ58541.1"
                     /translation="MKNQSELPFDAGIRIEVADRRIFVKMPKRDADVQFVRSFRFAYW
                     DAGALLWVIPHYRNNLELLKEYFAERLVECREMKALNAKGSVTAKIKANNSELLVIQT
                     STKQLRLIVALNPEFNRLIRELPYSRWDAKNRWWTAPFSDKIINRLKQIALDLGLQLR
                     LEQEEQGNKGKPRLSPFDVPNYRPCPEIYELKLRELRYSEHTIKSYTNLFEEFINYYH
                     RFDIDRIDETMITAFLRYLVIERKVSISYQNQAINAIKFYYERVLGGQRKVYLVDRPR
                     TETKLPVVLSQEEVCEILKATVNLKHKAILMTIYSAGLRISEAIQLKVSDIDSQRMQI
                     RITQAKGKKDRYTLLAVKTLDLLRQYVRMYKPKFWLFEGQKGEQYSNSSIQAILKVSL
                     SKTKIQKRVTVHTLRHSFATHLLENGTDLRYIQNLLGHGSSKTTEIYTHVTTRGFEQI
                     KSPLDRLNL"
     gene            complement(1872..3497)
                     /locus_tag="PLI37_05735"
     CDS             complement(1872..3497)
                     /locus_tag="PLI37_05735"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ58542.1"
                     /translation="MKTFSHLFLYLLLAALGFSCSNFFEVESKAYLDIEKNRLDTPND
                     SVYSVFGLLSGMQDIGPVCVLLGELQADLMDLTPNADADLKELNEHRASVDNPYLKAT
                     GFYRIINDCNYMIQHMDTVTGDKALLQDYAAAVAIRAWTYLQMSKIYGPVPYFDHCIL
                     DAEEALTADFPKMSPAELLPLLIAQVQDILHIPRPFRRMNSIPVGKMIPGNEFLLGEC
                     YLWLNDYENAALWYKRAIEKDNYRFVCNNSFTIAYEDDGSIVQSWYQIFENSNYARTY
                     ENNASVFYDKNYGKSNSLRTLYYGKEGSNKIRPSWAIIEDWIAQPLGTTNLELKKLGD
                     RRGLTNAWRFDKEAPENTIRPLINKFASDSIEVNIIIERAAMYHLRYAEAINRTGRPG
                     LALQMINKGPRPLYLNDSTLVKRQELAGKPEIVDFDHAAYATSMGIRGRVDLEPYTFD
                     AALPTLEDSVLFVEDKILQEAALELAFEGHRWFDLVRVAQRRPNPAAFLSGMVCRKFE
                     ASIDPLFPFEASADKATVKAAIESAGFFLPLPE"
     gene            complement(3508..6618)
                     /locus_tag="PLI37_05740"
     CDS             complement(3508..6618)
                     /locus_tag="PLI37_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOQ58543.1"
                     /translation="MSARNKIKSIRNLFLVCILLSVSSRAAASLTVEGKVLDATGKTP
                     VYAAQVRIENFSAAISDEEGNFSIKAPNENAVLRVSAWGYQVKHFPLKGRSKVVVLLV
                     SDDFKSLNPLLEFPFGEQALQSVPYAVSGFRSEPPAQSLSLTENMQKELGMLRSINRS
                     AAPASGKNTYLRGLNSLYGSSQPLYIIDGVPFETLTDKHSIIEGNYSDPLSTINPEDI
                     ESITLIRDAYALYGMKAANGAVLIRTRRAREQTSRISVGASWGVVSAPRQIPVLQSKD
                     YRAYALEQIGGAGYTEQEIQDQLYLSDDPSRPYYHTFHNNTNWQDEIFQNAMVQRYYG
                     QVTGGDDIAGYAISIGYDDAESVMKTGSMNRFSSRFNSDIKFTERLLFALGLSFSQVN
                     KQLRDDGCVQLSSPTYLSLIKSPLVSPYLLGEDGNRLPTDADEDFWGLSNPVSILLRG
                     LGENAQQRVDLNGKLEYSLNKQWMFTGLFGYNTDKLTESYFLPDYGVASIELERIRQV
                     SRNYVQNRSARYVGVYANLNAQYRGVWNYVHQLRLNAGLRYHSNQYLSQGGSGYNTVE
                     DKFIYLTNNLLAKAIFNEDNQWLWGSAYLDAQYTLKNTFDFSFNLSADASSRTGSKHR
                     GGLFSSAAASWLLSSEKFMAGARAIDVLKLRISAGRNGSDDLGPANPRGYFGSVSYMN
                     MMGLSLRNLNNEDLKYETTDKANLGLDLAVFNERLALSVDLYQHLTHDLITMRDAKQV
                     SGFDYVWVNEGSLQNRGLEASLNLRLVDTRKFKWNLQAGLGHYKNRLKDLANDIFTEY
                     SGATVLSRQGNPLGLFYGYRTQGVFSTYEEAQAANLKTLYNNSTVYQFKAGDMHFADL
                     NNDGWIDEKDRVIIGDPNPDFYGSLSTRLSYAGFSLDAVFSGVYGNDIYNHLRAGMEA
                     MDSFNNQSASVLNRWKGQGHAADIPRAAYNDPGGNARFSDRWIEDGSYLKLQKLGLEW
                     AAPGEVLFLDGLRVFIYAENLVTWTNYLGRDPDLSHSNYSLLQGIDRGYLPQSRSFMA
                     GIKINL"
     gene            complement(6637..8178)
                     /locus_tag="PLI37_05745"
     CDS             complement(6637..8178)
                     /locus_tag="PLI37_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOQ58544.1"
                     /translation="MKKTVTHIARFLPLLLLTAVACQDSWNRHYSYDKTLGGEDLLTT
                     LKAEASYSRFVKLIEIAGLQDKLAGSDSYTVWAPHNDFIPDSVYTSGDTTLIRDVVHN
                     HIAYFLHSTAEAYSGSSVMMLNGKYIHFGKGGSEKFAFGGFELDPADRVCSNGVLHGL
                     AGYVPFEYNLWEYLEKTPELSLLLQAYNFYDSTYMDRERSRRIGVDSMGNHVYDTVLI
                     QENTLLRRIGKIYNEDRNYTMIAPTNRAFEQAFDYYKNYYKSNGGPTVTMNKDSIQRT
                     RTHLALIRNLIFEGGFDTLTDTAVVSTFGYRFGNPGELFDGRRIETSNGDLFIVDSLR
                     FDPYELVYREMVYEVEKVCTPEDVTTANYTLLYPGENTKTRGISGGYFYITESSASRD
                     PKLTVRFGDSILSAKYDIYCTIVPGNTEDSTNLEEKTLLDFAIGYKNGHFARNVNLNK
                     AQETHPTELGLVQAGSEVNIPTFDSEVSLTITIKVPSAQRDKYKRNVRIDNVRLVPAG
                     RSPNF"
     gene            complement(8189..>8959)
                     /locus_tag="PLI37_05750"
     CDS             complement(8189..>8959)
                     /locus_tag="PLI37_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58545.1"
                     /translation="IKAGNGVIHELDGMMEIPDQASFFHKKMRFNVASFLPELMNYGA
                     RGQKVLELPDARLGGPQYFENMIIYGDAGFVLPRYDYTGSWIRHHWDEILIGMGYQAE
                     NWAQCNSATEYANTRYDVHLRIPPVPVGTWEIRFGFTTNQYRGMAQLYFDGIPAGVPL
                     SFLPTENYGISGMDEQTARKVLYNNGFMSFPTHITDASGNNTAYTRTDRMRRILGVFS
                     FSKMEPHVIRLKTVEPGQLSLNFVEYIPIEMVAEEGYD"
BASE COUNT         2092 a         2080 c         2397 g         2390 t
ORIGIN      
        1 gtaacaccca aataactcta ttgggtttat ttccgcttta ttcccagatc cggggctatc
       61 aaaaaagtgg ctgcgatagt ctgttgtgag caaagaataa tccatgtgta attggagtgt
      121 ttcgggttat tatggtatta attatctgcc aattagcttt tctcaaggca agtaagttta
      181 ataaacagct tgctttgggt tcggtaatcg actaacaaaa atacacatta attgtatttt
      241 cacatctgtt ttcgaagatt tttacaagtt caatctgtcc aaggggcttt taatttgctc
      301 aaagcctctg gtggtgacgt gtgtataaat ttcagtagtc ttggagcttc catgtcctaa
      361 caggttttga atatagcgta agtctgtacc gttctccagc aagtgggtgg cgaaactatg
      421 tcgcagggtg tgtactgtga ctcttttttg gatttttgtt ttagagagtg aaactttcaa
      481 aatggcttgg atactactgt tcgaatattg ttcccctttt tgtccttcaa acaaccagaa
      541 ttttggttta tacatgcgaa cgtattggcg caaaagatct aatgttttga cggccaataa
      601 ggtgtaacgg tcttttttac ctttggcctg ggtaattctg atttgcatgc gttgtgaatc
      661 aatatcactg actttcaact gaatcgcctc actgatacgc aggccggcag aataaattgt
      721 cattaagatt gccttgtgtt tcaggttgac tgtggctttc aaaatttcgc aaacctcttc
      781 ctgactgagc accaccggca atttggtttc cgttctgggg cggtcgacca gatagacttt
      841 tcgctgtccg ccaagaactc gttcataata gaacttgatg gcatttatcg cctggttctg
      901 ataggatatc gatactttac gttcaatcac cagataacgt aaaaaagccg tgatcatagt
      961 ttcgtcgatg cggtcaatgt caaaacggtg ataataatta atgaactctt caaaaagatt
     1021 agtatagctt tttatagtat gttcgctgta gcgaagttct ctcagcttca attcatatat
     1081 ctccgggcag gggcgatagt tgggcacatc aaaaggacta aggcgtggtt tgcccttatt
     1141 tccctgttct tcttgttcca ggcggagctg aagacccaga tcaagggcta tttgtttcag
     1201 tctgtttata atcttatcgg aaaaaggtgc tgtccaccaa cggtttttgg catcccagcg
     1261 agaataggga agttctctga ttaaacgatt gaattccgga ttgagggcaa ctattagcct
     1321 taattgtttg gtagaggttt ggataaccag gagctcacta ttgtttgcct ttatttttgc
     1381 agttacagat cccttagcat ttaaagcttt catttccctg cattccacaa gacgctcagc
     1441 aaaatattct ttcaataact ccaaattatt tctgtaatgg ggaataaccc aaagaagggc
     1501 gccggcatcc caataggcaa agcgaaaaga acgaacaaat tgtacatcgg catccctttt
     1561 gggcattttt acgaagattc gcctatcggc cacttctata cgaatccctg catcaaaagg
     1621 gagctcagac tggtttttca tataaacaac atactctgat gattaaactt tgaaatattg
     1681 tgtatatatg ccaaaaacag caaataacac caactcaaaa acagagtaaa gatacatata
     1741 attaagacgt aattaatgca tatttaatgt ttttagagat gaaaccgaat gaaacaccag
     1801 ccgtggatac cgggaggttg aacggtaggc cgttgagtga aagcaagcgc tctcggaaaa
     1861 cgaaaaccgg cttactccgg cagcggcaaa aagaagccgg ccgattcaat ggctgctttc
     1921 acggtggctt tgtctgccga ggcttcgaag gggaataggg gatcgatgct cgcttcaaat
     1981 ttgcggcaaa ccattcccga taaaaaggcg gcggggttgg gtcggcgttg ggctacacgt
     2041 accagatcga accagcgatg tccttcgaag gccagctcca aggcggcttc ctgcaagatt
     2101 ttgtcctcta caaacaaaac ggagtcttcc agggtgggca atgcggcgtc gaaggtatag
     2161 ggctccagat ccacacgtcc acggataccc atggaggtgg cgtaagcggc gtgatcaaaa
     2221 tccacaattt cgggtttacc ggccagctcc tggcgcttga ccagggtgct gtcgttcaga
     2281 tagagtgggc gagggccttt gttgatcatt tgcaaggcca gccccggacg gcctgtgcgg
     2341 ttgatggctt cggcgtaacg caaatgatac atggctgctc tttcgatgat gatattcact
     2401 tcgatagagt cgctggcaaa tttgttgatc agcggccgga tggtattttc gggggcctcc
     2461 ttgtcgaatc tccaggcatt ggtcaggcct ctgcgatcgc ccagtttctt caattcgaga
     2521 ttggtggtgc ccaaaggttg agcaatccag tcttcgataa tggcccacga aggcctgatc
     2581 ttgttactgc cttccttgcc atagtacaaa gtcctcaggc tgttggattt accgtagttt
     2641 ttatcgtaaa aaacggaagc attgttttcg taagtacggg cataattgga attctcgaag
     2701 atttgatacc aggattgaac aatactgccg tcgtcctcgt aggcgatggt gaagctgttg
     2761 ttgcagacaa agcggtaatt gtctttttcg atggcccttt tgtaccacaa agcagcgttc
     2821 tcgtaatcgt tgagccacaa ataacattcc cccagcaaaa actcgttgcc cggaatcatt
     2881 ttgcccaccg gaatgctgtt catccggcga aaaggcctgg gaatatgtag aatatcctgt
     2941 acctgagcaa taagcagggg caggagttcc gccggactca ttttgggaaa atcggcggtg
     3001 agcgcctctt ccgcgtcgag aatgcaatga tcgaaataag gcacagggcc gtagatctta
     3061 ctcatttgca aataggtcca ggctctgatg gccacggctg cggcgtaatc ctgcaacaaa
     3121 gccttgtcgc ccgtcaccgt atccatatgc tgaatcatat agttgcagtc gttgatgata
     3181 cgataaaagc cggtggcttt caggtaggga ttatcgacgc ttgccctgtg ttcgttgagc
     3241 tctttgaggt ctgcatcggc gttgggagtc aggtccatca gatcggcctg cagttcaccc
     3301 aaaagaacgc agacaggccc gatgtcctgc atgcccgaca acaaaccaaa cacggaataa
     3361 accgagtcgt tgggcgtgtc cagccggttt ttttcgatat cgaggtaggc cttggattcg
     3421 acttcgaaaa agtttgaaca ggaaaaaccc aaagcagcca ggagcaagta taggaagagg
     3481 tgagaaaaag ttttcatagt tcgttgctta gagattgatt ttgataccgg ccataaacga
     3541 tcgggactga ggcaggtaac cccggtcgat gccttgcagc agactgtagt tggaatggct
     3601 caggtccgga tccctgccta ggtaattggt ccaggtgacg aggttttcgg cgtagataaa
     3661 cacgcgcagg ccgtcgagga agaggacttc gccgggagcc gcccattcga ggcccagttt
     3721 ctgcagcttc aggtaggaac cgtcttcgat ccagcgatcc gagaagcggg cgttgccccc
     3781 gggatcgttg taggcagccc tgggaatatc ggcagcgtgg ccctggccct tccagcgatt
     3841 cagcacgctg gccgactggt tgttgaaact gtccatagct tccatcccgg ctctgaggtg
     3901 gttgtagatg tcgttaccgt acactccgct gaatactgca tcgaggctaa agccggcgta
     3961 gctcaggcgg gtcgataggc tgccgtaaaa atcgggattg gggtcgccga tgattacacg
     4021 gtctttttcg tctatccagc cgtcgttgtt tagatcggca aaatgcatgt caccggcctt
     4081 gaattgatag accgtactgt tgttgtacaa agtcttgaga ttggcggcct gagcttcttc
     4141 gtaggtgctg aagactccct gggtccggta accgtaaaat aggcccaggg gattgccttg
     4201 ccgcgagagt acggtagctc cggaatattc ggtaaaaata tcgttcgcta agtctttcag
     4261 cctgttttta taatgcccca gaccggcttg caggttccat ttgaacttgc gggtatccac
     4321 caacctcagg tttagagaag cttccagtcc ccggttttgc agcgaacctt cgtttaccca
     4381 gacgtaatcg aagcccgaca cttgtttggc atcgcgcata gtgattaaat cgtgggtaag
     4441 atgctgatac aggtcgaccg ataaagccag cctctcgtta aacacggcca gatccagacc
     4501 cagatttgct ttgtcggtgg tttcgtattt gagatcttcg ttgttgaggt ttctcaggct
     4561 cagccccatc atattcatgt aactgaccga gccgaaatag ccgcggggat tggccggacc
     4621 cagatcgtcg ctgccgttgc ggccggcgct tattctgagt ttcagcacat cgatagcccg
     4681 tgcaccggcc atgaattttt cggaagaaag caaccaggaa gcagcggccg aagaaaagag
     4741 tccgccgcgg tgtttgctgc ccgttctcga gctggcgtcg gccgagaggt tgaacgaaaa
     4801 atcgaaggtg tttttcagcg tgtattgagc gtccaaatag gcagaacccc agagccattg
     4861 gttgtcttcg ttaaagatag ccttggccag caggttgttg gtgagataga tgaatttatc
     4921 ttctacggtg ttgtagcccg aacctccctg tgagaggtat tgattggaat gataacgcag
     4981 ccccgcgtta agccttagtt gatgaacgta attccagact ccgcggtatt gggcgttcag
     5041 gttggcatac acgccaacgt aacgggcgga acggttttga acgtaatttc tggatacttg
     5101 cctgatgcgt tccagctcta tactggccac tccgtagtcg ggcagaaaat acgattcggt
     5161 cagtttgtcg gtgttgtagc cgaaaagtcc ggtaaacatc cattgcttgt ttaggctgta
     5221 ttcgagcttg ccgttcaggt cgaccctttg ctgagcattt tcacccaggc cccggagcag
     5281 gatagacacc gggttgctga ggccccagaa atcttcgtcg gcatcggtgg gcaggcggtt
     5341 gccgtcttcg ccgagcaggt agggcgacac caggggcgat ttgatcagcg acaggtaagt
     5401 aggcgaggag agttgcacgc agccgtcgtc gcgcaattgc ttgttcacct ggctaaacga
     5461 caaacccagc gcaaagagca aacgttcggt aaacttgatg tcgctgttga agcgggaact
     5521 gaagcggttc atgctgcctg ttttcataac gctttccgca tcgtcgtagc ctatggaaat
     5581 ggcgtaaccg gcaatgtcgt cgccaccggt cacctgaccg taataacgtt gaaccatagc
     5641 gttttgaaaa atttcgtcct gccagttggt gttgttgtgg aaggtatggt aatagggcct
     5701 ggagggatcg tcgctcaggt agagctgatc ctgaatttcc tgttcggtat agccggcacc
     5761 gccgatttgt tccaaggcat aggcccggta atctttgctc tgcaggacgg gaatttgcct
     5821 gggagccgaa accactcccc aggaagcacc caccgaaatg cgcgaagttt gttccctggc
     5881 tcttctggtt ctgatcagaa cggctccgtt ggctgctttc atgccgtata gagcgtaggc
     5941 atcgcggatc agggtgatgc tttcgatgtc ttcgggattg atggtgctca ggggatcgct
     6001 gtaatttcct tcgatgatgc tgtgtttgtc ggtcagggtc tcaaagggaa caccgtcgat
     6061 gatgtagaga ggctgggaac tgccgtacag actattgagt cctctcagat aggtgttttt
     6121 gccgctggcg ggagccgccg aacggttgat actgcggagc attccgagtt ctttttgcat
     6181 attctctgtc aggctcaggc tttgcgccgg cggttcggac ctaaagccgc tgacggcgta
     6241 gggaacgctt tgcagggcct gttccccaaa tggaaattcg agcaggggat tcaggctttt
     6301 aaaatcgtcc gatacgagta atacaactac cttgctgcgt cctttcaggg gaaagtgttt
     6361 aacctgatag ccccaggcgc tcacgcggag cacggcattt tcgttggggg ctttgatgct
     6421 gaaatttccc tcttcgtcgc tgatggccgc cgagaaattt tctattctga cctgagcggc
     6481 gtaaacaggt gttttgcccg ttgcatccaa tactttacct tctactgtca gtgaggcggc
     6541 agcccggctg ctgacactca ggaggataca gaccaaaaac aggttccgga ttgattttat
     6601 tttgtttctt gctgacataa tcgctgatga caaagattaa aaattgggag atcgacctgc
     6661 aggcaccaga cggacgttgt cgatacgaac gttgcgttta tatttgtccc gctgtgcgct
     6721 gggtacttta atggtaatgg tcagggagac ttcgctgtcg aaggtgggaa tgttcacttc
     6781 gctgccggcc tgcaccaagc caagctcggt gggatgggtt tcctgtgcct tgttcaggtt
     6841 gacgttacgg gcaaagtgtc cgtttttgta accgatggcg aaatccagca gggttttctc
     6901 ttccagattg gtgctgtctt cggtattgcc cggcactatg gtgcagtaaa tgtcgtattt
     6961 ggccgaaaga atgctgtcgc caaaacggac ggtcagtttg ggatcgcggc tggcggagct
     7021 ttcggtgata tagaaataac cgcccgaaat gccccgggtc ttggtgtttt ccccgggata
     7081 cagcagggtg taattggccg tagtcacgtc ttcgggggtg cagacttttt ccacttcgta
     7141 caccatttcg cggtacacca gttcgtaggg atcgaaacgc agggaatcga caataaacaa
     7201 gtctccgttg ctggtctcta tacggcgtcc gtcgaacaat tctccgggat taccgaaacg
     7261 atagccaaag gtggaaacca ccgccgtatc ggtgagtgta tcgaagccgc cctcgaagat
     7321 caggtttctg atcagagcca ggtgggtgcg ggtgcgctgt atggaatcct tattcatggt
     7381 taccgttggg ccgccgttgg atttatagta atttttgtaa taatcgaagg cttgttcaaa
     7441 ggcccggttg gtgggagcga tcatggtgta gttccgatct tcgttgtaga tttttccgat
     7501 gcgcctgagc agggtattct cctgaatcaa caccgtgtcg taaacgtggt tgcccatgga
     7561 atcgacacct atgcgtctgc tgcgttcgcg gtccatatag gtggaatcgt aaaagttgta
     7621 ggcttgcagc aagaggctca gttcaggcgt tttttcgagg tattcccaga ggttgtattc
     7681 gaaaggtaca tagccggcga gtccgtgcaa aacaccgttg gaacagaccc ggtcggccgg
     7741 atccagctcg aaacccccaa aggcgaattt ctcgctgccc cctttgccga agtggatgta
     7801 ttttccgttg agcatcatta cactgctgcc cgaataggct tcggcagtag aatgcaagaa
     7861 ataggcgatg tggttgtgaa cgacatccct gatcagggta gtatctcccg aggtatatac
     7921 cgaatcggga ataaaatcgt tgtgcggagc ccacaccgtg taagagtccg aaccggccag
     7981 tttgtcctgc aggccggcga tctcgatgag ttttacaaag cggctgtagg aagcctcggc
     8041 tttgagcgta gtcagcaggt cttcgcctcc cagggttttg tcgtagctgt aatgccggtt
     8101 ccaactgtcc tggcaggcta cagccgtcag caagagcaga ggtaaaaacc gggcgatatg
     8161 tgttactgtt tttttcatag tgcagtgatt aatcgtatcc ttcttcggca accatttcga
     8221 ttggaatgta ttcaacaaaa ttcagcgata actgccccgg ttcaaccgtc ttgaggcgta
     8281 taacgtgcgg ttccatcttt gaaaagctga aaacgcccaa aatgcggcgc attcggtcgg
     8341 tacgggtata ggccgtattg ttgccgctgg cgtcggtgat atgggtgggg aacgacataa
     8401 aaccgttgtt gtaaagcacc ttgcgggcag tttgttcgtc catgccgctg ataccgtagt
     8461 tctcggtggg caggaaacta agaggcactc cggccggtat gccgtcgaaa tacaactggg
     8521 ccatgccccg gtattggttg gtggtaaaac cgaaacggat ttcccaggtt cccaccggca
     8581 cgggcggaat acgcagatgg acgtcgtagc gggtattggc gtattcggtg gcgctgttgc
     8641 actgtgccca gttttcggcc tgataaccca tgcctatcag aatttcgtcc cagtggtgcc
     8701 ttatccagga accggtgtag tcgtagcgtg gcagtacaaa gccggcatcg ccgtagatga
     8761 tcatgttttc aaaatactgg ggtcctccga ggcgggcatc gggcaattcg aggacttttt
     8821 gcccccgtgc tccgtagttc atcaattcgg gcaggaaact ggcgacattg aaacgcattt
     8881 ttttgtggaa aaagctggcc tgatccggaa tttccatcat tccgtccagt tcgtgtatca
     8941 ctccgttacc ggctttgat
//
LOCUS       DAOSTD010000072         8884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_7498_length_8884_cov_13.836448, whole genome shotgun sequence.
ACCESSION   DAOSTD010000072 DAOSTD010000000
VERSION     DAOSTD010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7498_length_8884_cov_13.836448"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..846
                     /locus_tag="PLI37_05755"
     CDS             145..846
                     /locus_tag="PLI37_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ58546.1"
                     /translation="MNRTILHVENDSDYAKLIGELLKKKGHNVVHASCGRKGLKLLKH
                     CSPDIVLLGIIMPDIDGFTFAEIARESDPTLPIVFLTSLSDPKNEIKGLKLGACDYIC
                     KEADLDVVLARIEKELKRCHKRYISIQLTPDTSIDTKNKMLIIRGKRHKLGMRDFDLL
                     IFLCDTIDVVHHRKIIEQTIWGENANSTQYLYKATYNLRKILQYDPTLSIDTFRNGDI
                     VLNNTSASRIPYHVK"
     gene            977..2224
                     /locus_tag="PLI37_05760"
     CDS             977..2224
                     /locus_tag="PLI37_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOQ58547.1"
                     /translation="MKIGKYSFGTGDRFAHQGEAQLKALLKARKELGLDITPVWNKSN
                     REHMIVNSSPADTRIEADAAVKALGYTGPYFVDADHINMSNVEKFVDSSDFFTIDLAD
                     FIGKKAAQSDIDAFIKRNAKYLGKLNIPGIPEAFVITDAVLKAAAEKFLFAAQEAGRI
                     YRYIAGRKGAGNFIPEVSMDEVNDAQTPVEMFFILSALAAEKVPAQTIAPKFTGRFNK
                     GVDYVGDIAQFAKEFEQDLLVIDFAVKEFGLPDNLKLSIHSGSDKFSIYPVMGELIRK
                     YDKGIHVKTAGTTWLEEIIGLAMADEEALDLAKAIYESALGRFDELCGPYATVIDIDK
                     KQLPTPKEVEKWTGEKFANTLRHIPDNPDYNPHFRQLIHVGYKVAAEYGKEYTDALKR
                     NKAVVAEQVIANIYDRHILRMFA"
     gene            2246..3097
                     /locus_tag="PLI37_05765"
     CDS             2246..3097
                     /locus_tag="PLI37_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ58548.1"
                     /translation="MNKFTFEDLKGKVCVLTGGAGVIGASLARGLASAGVKTAIVDIN
                     EEAAVKLAAQVEKESGVKCIGISANVLDKESLEAAKSVINQKLGKIDILINGAGGNSP
                     KATTAVEQMQKEDLTDLSKTFYGLGMDGFQRVYDLNFKGTLLPTMVFTLDMLENGKGC
                     VLNISSMNAYRPLTKIPAYSAAKASVNNLTQWMSVHLAKVGIRVNAIAPGFFLTNQNR
                     FLLTDEKTGEATPRGHKIISNTPMGKYGEPEDLIGAMLYLLSDISSFVTGVIIPVDGG
                     YSAFGGV"
     gene            3116..4387
                     /locus_tag="PLI37_05770"
     CDS             3116..4387
                     /locus_tag="PLI37_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOQ58549.1"
                     /translation="MLYYSRGSKNDSIGPEELRKILYEVFKRLGPKKRVLAIPPDFTR
                     FHSFAGQITEMVWDYYGQTLTDVMPALGTHVPMTEKEIKTMFGNIPLELFRVHDWRND
                     VVTVGEVSASYVHEVSEGKLNYAWPAQVNKLLLDPEFDLILSVGQVVPHEVVGMANYN
                     KNIFVGTGGSEGINKSHYIGATYGMERLMGRADSPVRKVLEYASKNFTAHLPLIYIQT
                     VVSKNEEGNLVIRGIFIGDDFECFQKAADLSMEVNFLMVEKPIRKCVVWLDPSEFKST
                     WLGNKSIYRTRMALADGAELIVLAPALKEFGEDKTIDGLIRKYGYAGTPAILKAVEEN
                     EDLRDNLGAPSHLIHGSSEGRFTITYCPGHLSREEIESVHFNYGDMQEMMKRYNPEEL
                     KDGINIMPDGEEIFYISNPALGLWAHKDRFN"
     gene            4392..>4666
                     /locus_tag="PLI37_05775"
     CDS             4392..>4666
                     /locus_tag="PLI37_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythronate-4-phosphate dehydrogenase"
                     /protein_id="HOQ58550.1"
                     /translation="MKIVCDHKIPFLKGALEPYAEVVYLPGNRIAAADVKDADALITR
                     TRTKCNAALLEHSAVKMIATATIGYDHIDTEWCEQKGIAWTNAPGCNS"
     assembly_gap    4667..4766
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4767..5372
                     /locus_tag="PLI37_05780"
     CDS             <4767..5372
                     /locus_tag="PLI37_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3410 domain-containing protein"
                     /protein_id="HOQ58551.1"
                     /translation="TKGPDATYHLFDAKRLQGLSPHQILINSSRGEVVDNQALKACLK
                     QGRIKAAVLDVWEKEPAIDLELLDLVLYGTPHIAGYSADGKGNGTTMSVRAIAKKFNL
                     PLQDWQAAHLPLPAQSIYPELNGRGKSPQEVLCKAILHTYRVEDESRVLKQNPGTFEQ
                     QRADYAVRREFGCYTLRLRGVEKPVAEKLASLGFKLEILPE"
     gene            5408..6859
                     /gene="gnd"
                     /locus_tag="PLI37_05785"
     CDS             5408..6859
                     /gene="gnd"
                     /locus_tag="PLI37_05785"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOQ58552.1"
                     /translation="MKQLSDIGLVGLAVMGENLVLNMESKGFTVSVFNRTVAKVDNFV
                     NGRGKGKNIIGTRSIEELVASIKRPRKVMLMVKAGKAVDDFIELLLPHLEPGDIIIDG
                     GNTHYPDTNRRTAYLESKGFLYIGTGVSGGEEGALTGPSIMPGGSKAAWPHVKPIFQA
                     IAAKVEDGPCCDWVGENGAGHFVKMVHNGIEYGDMQLICEAYHIMKDMLGMNTDEMHE
                     VFAEWNKGDLDSYLIEITRDILAYKDENNQPVVEYILDTAGQKGTGKWTGIAALEHGV
                     PLTLIGEAVFSRCLSAQKDERVAASKILQGPKPAFKGDKKAFIEDLRKALFASKVVSY
                     AQGYTLMRSAAEEYGWDLNYGGIALMWRGGCIIRSVFLGKIKEAFDKDHDIPNILIAP
                     YFTEKLKEAQNSWRNVVAQTALNGIPAPALSSALAYYDGYRCERLPANLLQAQRDYFG
                     AHTYERIDKPRGEFFHTNWTGRGGDTVATTYIA"
     gene            7053..7946
                     /locus_tag="PLI37_05790"
     CDS             7053..7946
                     /locus_tag="PLI37_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOQ58553.1"
                     /translation="MKQQINSFALPFAMLTGGIFHTFFNQLNFLTPYLIFFMLFFPYC
                     RIQLKDMRLRSFHLWLILYQVAGSLLAYLLLSLFSEELGQGVLICILAPTATAAIVVG
                     GMLGANIALMASYTLFCNLVVAIMAPILFSFVGIQAELYFWTSFWLIFKRVFPLLVLP
                     FVAALLLRKFAPGFNSKIQKMSSISFYLWIFALAIVSGRIVHFMLDQQKSDYRMAAVL
                     AAAALVICVFQFLFGRFVGRRYGETVSGGQSLGQKNTILAIWMAQTYLNPIAAIAPAS
                     YVLWQNLVNSYQLWRKSRKTS"
     gene            7959..8843
                     /locus_tag="PLI37_05795"
     CDS             7959..8843
                     /locus_tag="PLI37_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HOQ58554.1"
                     /translation="MKKNIKIALIQTAVSADPEQNKSMLAQKIADCRRQGAELVVLQE
                     LHNTLYFCQEESTDLFDLAESIPGPSTAFYSKLAAQHRLMLVCSLFERRAAGLYHNTA
                     VVFERDGSLAGTYRKMHIPDDPGFYEKFYFAPGDLGFKPIETSLGKLGVLICWDQWFP
                     EAARLMTLKGADLLIYPTAIGWDLRDDEQEKARQLDAWITVQRGHAVANGLPVVAVNR
                     SGYEQSRADSQQGILFWGNSFVAGPQGEIQTRAGEKDEILLTDINLDRNEEVRRWWPF
                     LRDRRIDAYQNLLKRFVD"
BASE COUNT         2317 a         2174 c         2088 g         2205 t
ORIGIN      
        1 gcgacaaaca taaaaacaat tttccctata tatttacctt atatttacct tttttgggct
       61 gtttattgaa catttttcta ctatatccaa ttgtttttcc atatatttgc aggtttttac
      121 aaaagatatg tttaatacca aatcatgaac cgcaccattc ttcatgtcga aaacgacagc
      181 gattatgcga aattgatcgg cgaattattg aaaaaaaaag gccataacgt cgtacacgct
      241 tcctgtggta gaaaaggact taaactgctc aaacactgtt cacccgacat cgtattactt
      301 gggatcataa tgcccgatat agacggtttt accttcgccg aaatagcgcg ggaatccgat
      361 ccgactcttc ccatagtgtt tcttacttct ctgtctgatc cgaaaaatga aatcaaaggc
      421 cttaaattgg gagcctgcga ttacatttgc aaggaagccg atttagacgt tgtacttgct
      481 cgcatcgaaa aagagttgaa aagatgccac aaaagatata tctccataca acttacgccg
      541 gatacttcca ttgatactaa aaacaaaatg ctgattatta gagggaaaag acataaattg
      601 ggaatgcgcg attttgatct gctgatattt ttatgcgata ctatagacgt ggttcaccac
      661 agaaaaatta ttgagcaaac catctggggc gaaaacgcca actcaacgca gtacctctac
      721 aaagcgacct acaatttgag gaaaatactg caatacgatc ctactctctc catcgatact
      781 tttcgaaacg gcgacatcgt gttgaacaat acgtccgcat cccgcatccc ttatcacgta
      841 aaataagttt aaaatacggt tgatatcttg ttttttttga aaaggcttcg cttttggtaa
      901 aaaaatccgt tatttttgtg gaaaatattt tcattactaa cggtggctta tgaccgccct
      961 ttaatccttt attatcatga aaatcggtaa atattctttt ggcacaggcg accgctttgc
     1021 ccatcagggc gaggcccaac tgaaagcatt gttgaaagcc cgcaaggagc ttggtttgga
     1081 catcactccc gtttggaaca aatcgaaccg cgaacatatg atcgtaaatt cttcgccggc
     1141 agacacccgc atcgaagccg atgctgccgt aaaagccctg ggctacacag gtccttattt
     1201 tgtagatgca gaccacatca acatgtctaa tgtcgaaaaa tttgtagatt cttccgactt
     1261 tttcaccatc gatttggctg attttatcgg caagaaagcc gcacaaagcg acatcgacgc
     1321 cttcattaaa cgcaacgcca aatacctggg taaattaaat atccccggca tccccgaagc
     1381 ttttgtcatt accgatgccg tactgaaagc agccgccgaa aaatttcttt ttgccgccca
     1441 ggaagctggt cgcatttacc gctacattgc cggacgcaaa ggtgccggca acttcattcc
     1501 cgaagtatcg atggacgaag tcaacgacgc ccagactccg gtagaaatgt tttttattct
     1561 gagcgccctg gcagccgaaa aggtgcctgc ccaaacaatt gcgcctaaat tcaccggccg
     1621 cttcaacaaa ggagtcgatt acgtaggcga tatagctcaa tttgccaagg aatttgaaca
     1681 agacctcctg gtgattgatt ttgccgtcaa ggaattcggc ttgcccgaca acctgaaact
     1741 gagcattcac tccggcagcg acaaattctc catttatccc gttatgggcg agctgattcg
     1801 caaatacgac aaagggattc acgtcaagac agccggaacc acctggctcg aagaaatcat
     1861 cggcctggcc atggccgacg aagaggcgct ggatctggcc aaggccattt acgaaagcgc
     1921 gctgggacgt ttcgatgagc tctgcggtcc atacgccact gttattgaca tcgacaaaaa
     1981 acaactgcct acgcccaaag aggttgaaaa atggactggc gaaaagtttg ccaacacgct
     2041 gcgtcatatt ccggataatc ccgattacaa cccccatttc cgtcaactga ttcacgtagg
     2101 ctataaagtt gctgccgagt acggcaagga atacaccgat gccctcaaac gtaacaaagc
     2161 cgtggtagca gaacaagtca ttgccaacat ttacgatcgg catattttgc gcatgttcgc
     2221 ttaattgcca taaaaacact atattatgaa caaattcaca ttcgaagacc ttaaaggtaa
     2281 ggtctgtgtc ctgaccggag gtgccggtgt tattggcgca tcattggcaa gaggcctggc
     2341 ctctgccggt gttaaaaccg ccatagtaga tatcaacgaa gaagctgccg taaaactggc
     2401 cgctcaagtc gagaaagaat ccggcgtgaa atgcataggt atttcggcca atgtattgga
     2461 caaagagtcg ctcgaagcag ccaagtccgt tatcaaccaa aagttgggta agatcgatat
     2521 tttgatcaat ggagccggtg gcaattctcc caaagcaacc accgcggttg agcaaatgca
     2581 aaaagaagac ttaaccgatt taagcaaaac tttttacggt ttaggcatgg atggtttcca
     2641 aagagtgtac gatcttaact tcaaaggcac cttgcttccc accatggtct ttactttaga
     2701 catgctcgaa aacggcaaag gctgtgtact gaatatttct tcgatgaatg cttaccgccc
     2761 gctgaccaaa atcccggctt attcggctgc caaggcttct gtcaacaacc tcacccagtg
     2821 gatgtcggtt cacttggcca aagtgggtat ccgtgtcaac gccattgctc ccggcttttt
     2881 cctgaccaac cagaaccgtt ttctgctcac cgacgaaaaa acgggagaag ccaccccccg
     2941 cggtcacaaa attatttcca atactcccat gggaaaatac ggcgagcccg aagacctgat
     3001 cggcgccatg ttgtacctgt tgagcgatat ttcttctttt gtcaccggtg ttattattcc
     3061 ggtggacgga ggctacagtg cttttggtgg agtttagtat taaaaaaaga taagtatgtt
     3121 gtattatagc agaggctcca aaaacgacag catcggtccg gaagaactga gaaagatcct
     3181 gtacgaggtt tttaaacgtt taggccccaa aaagcgggtc ttggccattc cgcccgattt
     3241 cacccgcttc cattccttcg ccggacagat taccgaaatg gtctgggatt attacggaca
     3301 aaccctgacc gatgtgatgc cggctctggg aactcatgtg ccgatgaccg aaaaggaaat
     3361 aaaaaccatg ttcggcaata ttcctttaga attgttccgt gtacacgatt ggcgcaacga
     3421 cgtggtaacc gtgggcgaag tgtccgcttc ctacgtacac gaagtgtccg aaggtaaatt
     3481 gaactacgcc tggccggccc aggtaaacaa gcttttgctg gatccggaat ttgacctgat
     3541 tttgtccgtc gggcaagtgg taccgcacga agtggtgggc atggccaatt acaataaaaa
     3601 catttttgtg ggaaccggcg gttccgaagg catcaacaag agccactata tcggcgccac
     3661 ttacggaatg gaacgtctta tgggcagggc cgattcgccg gtgcgcaaag tactcgaata
     3721 cgcttctaaa aacttcaccg cccatctacc gctgatttat atccaaaccg ttgtatccaa
     3781 aaacgaagag ggcaacttag tgatcagggg tatttttatc ggtgacgatt ttgaatgttt
     3841 ccaaaaggcc gccgatctct ctatggaagt caatttcctg atggtagaga aacccattcg
     3901 caaatgcgtc gtttggcttg atccctcgga attcaaaagc acctggttgg gtaacaagag
     3961 catttaccgt acccgtatgg ccctggccga cggagcggaa ctgattgttc tggccccggc
     4021 cctgaaggaa ttcggcgagg acaaaaccat cgacggactc atacgcaaat acggttatgc
     4081 cggtaccccc gccatactca aggccgtgga agaaaacgaa gacctgcgcg acaacctggg
     4141 cgctccttct cacctgattc acggatcgag cgaagggcga tttaccatca cctattgccc
     4201 gggccacctg agccgcgaag aaatcgaaag cgttcacttc aattacggcg atatgcaaga
     4261 gatgatgaaa cgctacaatc ccgaagagct gaaagacggc atcaatatta tgcccgacgg
     4321 agaagaaatt ttctatatct ccaatccggc tttgggtttg tgggcacata aggacagatt
     4381 caactaaggg gatgaagata gtatgcgatc ataagattcc tttcctcaaa ggtgcattgg
     4441 aaccttatgc tgaagtcgtt tacctgcccg gcaaccggat tgcggctgca gatgtaaaag
     4501 acgctgatgc cctgattacc cgcacacgaa ccaaatgcaa tgccgccttg ttggagcatt
     4561 cagcggttaa aatgattgct accgccacca tcggttacga tcatatcgat accgaatggt
     4621 gtgagcaaaa gggcatagcc tggaccaatg ccccgggttg taactcnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnacaa aaggcccgga tgccacttat cacctgttcg
     4801 acgccaaacg cctgcagggc ttgtcgccgc atcagatact catcaattcc tcgagaggcg
     4861 aagtggtcga taatcaggcc ctgaaagcct gcctgaagca aggcagaata aaagcggcgg
     4921 tgctcgatgt ctgggaaaag gagccggcca tcgatctgga actactggac ctggtgcttt
     4981 acggaacacc ccatatagcg ggttattccg ccgacggcaa aggcaacggc accactatga
     5041 gtgtaagggc catcgccaaa aagttcaatc tgccgctcca agactggcag gccgcccact
     5101 tgcccctgcc cgcgcaaagc atctatcccg agcttaatgg cagaggcaag agcccgcagg
     5161 aagtgttgtg caaggctatt ttgcatacct acagggtgga agacgaaagc cgtgtgctaa
     5221 aacaaaaccc cggcaccttc gaacaacaac gggccgacta cgccgttcgc agagaattcg
     5281 gctgctatac ccttcgcttg cgaggagtgg aaaagcctgt cgcagagaag cttgcttcct
     5341 taggcttcaa attggaaatc cttcctgaat aaaaacaaat tataataact atttaaataa
     5401 cacgaacatg aaacaactat cagacattgg attagtagga ctggccgtta tgggcgagaa
     5461 cctcgtattg aacatggaaa gcaaaggctt taccgttagc gtattcaacc gcactgtggc
     5521 caaagtagat aattttgtca acggacgcgg caagggtaaa aacatcatcg gcacccgtag
     5581 catcgaagaa ttggtggctt cgatcaaacg cccccgcaaa gtaatgctca tggttaaggc
     5641 aggcaaggca gtagacgatt ttatcgaatt gctgcttccc catttggagc ccggcgatat
     5701 tattatcgac ggaggtaaca cccattaccc cgataccaac cgcaggacag cctacttgga
     5761 aagcaaagga tttttgtaca tcggcaccgg tgtttcgggc ggagaagaag gcgctcttac
     5821 cggtccttcc atcatgcccg gcggatcgaa agctgcctgg ccccatgtta aaccgatttt
     5881 ccaggccatt gccgccaagg tcgaagacgg cccctgctgc gactgggttg gtgaaaacgg
     5941 agccggccat tttgtcaaaa tggtacacaa cggcatcgaa tacggcgata tgcagcttat
     6001 ctgcgaagcc tatcatatca tgaaagacat gctgggcatg aataccgacg aaatgcacga
     6061 agtctttgcc gaatggaaca agggcgatct ggacagctac ctgatcgaga tcacccggga
     6121 tattctggct tacaaggacg aaaacaatca accggttgtg gaatacatcc tcgataccgc
     6181 cggccaaaaa ggcaccggca aatggactgg cattgccgcc ctcgagcacg gtgtgcctct
     6241 taccttgatc ggcgaagctg ttttctcccg ttgtttgtcc gctcaaaaag acgaacgcgt
     6301 tgctgcttcc aagattttgc aaggccccaa acctgctttc aagggcgata aaaaagcctt
     6361 tatcgaggac ttgcgcaaag ctttgtttgc ctccaaggtg gtttcgtatg cccagggtta
     6421 caccctgatg cgttcggctg ccgaagaata cggatgggat ctgaattacg gcggtattgc
     6481 cctgatgtgg cgaggcggtt gtattatccg ctcggtcttc ctgggcaaaa tcaaagaagc
     6541 cttcgacaaa gaccacgata ttcccaatat cctgattgct ccctatttca ccgaaaaatt
     6601 gaaggaagct caaaacagtt ggcgtaacgt tgtagctcaa acggctctga acggtatccc
     6661 cgctcccgcc ctgagttctg ctctggctta ttacgacggt tatcgctgtg agcgccttcc
     6721 ggccaacctc ttgcaggcac aacgcgacta tttcggcgca cacacctacg aacgcatcga
     6781 caagccccgc ggtgaatttt tccacaccaa ctggaccggt cgtggcggtg atacggtggc
     6841 caccacttat atcgcttaac ttttccaaac gatagccgaa ttaatcggca attgttttaa
     6901 cattttatga gaatcccaaa agtctgaatg gaacttttgg gattctcttt ttgagcttac
     6961 ttctaacatt ttgttttggg agctttactt tctgttttgc ttttgtcgtc gtttttttgg
     7021 taattttgcc gcctgatgtc cgatttgcct ctatgaaaca acaaataaac agctttgccc
     7081 tgccgttcgc tatgttgacg ggaggtatct ttcatacctt ttttaatcag ttgaatttcc
     7141 tcacccctta cctgattttt ttcatgctct ttttccccta ttgccgcatt cagctcaaag
     7201 atatgcgcct gaggagtttt cacctttggc tcattctgta ccaggtggcc ggcagtctgc
     7261 tggcctatct gctattgagt ctgttcagtg aagagctagg ccagggggtt ttaatctgca
     7321 tactggctcc cacggctact gcggctattg tggtgggtgg tatgctgggc gccaatattg
     7381 ccctgatggc tagctatacc ttgttttgca atctggtagt ggcgattatg gctcccattc
     7441 tgttctcttt tgtgggcata caggcggaat tgtatttctg gacttcgttt tggctgattt
     7501 ttaaaagggt cttccccctg ttggtgcttc cctttgtggc ggccttgctt ttgcgaaaat
     7561 tcgctcccgg ctttaatagt aaaattcaaa aaatgagttc tatctcgttt tatttgtgga
     7621 tatttgccct ggcgattgtg agcggacgca ttgtgcactt tatgctcgac cagcaaaaat
     7681 cggattacag gatggctgcc gtgctggcag cggcggcttt ggtaatttgt gtgtttcagt
     7741 ttctgttcgg acgctttgta ggccggcgtt acggcgaaac cgtcagcgga ggtcaatcgc
     7801 tgggacaaaa aaacaccatc ctggccatct ggatggctca gacttacctg aatcctattg
     7861 cagctatcgc tcctgcctct tacgtgttgt ggcagaattt agtgaacagc tatcaattgt
     7921 ggcgtaaaag cagaaaaacg tcttaatttt ttagccctat gaagaagaac atcaagattg
     7981 ccctgattca aaccgccgtt tcggccgatc cggaacaaaa caaatctatg ctggcgcaaa
     8041 aaatcgctga ttgccggcga caaggcgcgg aattggtggt tttgcaagaa ttgcataata
     8101 ccctgtattt ctgccaggag gaatccaccg acctgtttga cctggccgaa agcattcccg
     8161 gtccctctac cgcgttttac tcgaagttgg cggcacagca ccggctgatg ctggtttgtt
     8221 ctttgttcga gcgcagggcg gcgggtttgt accacaatac ggcggtggtg ttcgaacggg
     8281 acggttcttt ggccggcacc taccgaaaga tgcacatccc cgatgatccg ggcttttacg
     8341 aaaagtttta ttttgctccc ggcgatctag gcttcaagcc tatagaaacc tcgctgggga
     8401 aattgggcgt cctgatctgc tgggatcagt ggtttcccga agcggcccgt cttatgaccc
     8461 tgaagggagc cgatttgctt atttacccca cagccatcgg ctgggatttg cgcgacgacg
     8521 agcaggaaaa agcgcgtcag ctcgatgcct ggataacggt tcaacgggga catgccgtgg
     8581 ccaacggcct gccggtggtc gccgtaaaca gatcgggtta tgaacaaagc cgggccgatt
     8641 cccaacaagg catcttgttt tggggcaaca gcttcgttgc cgggcctcag ggggaaatac
     8701 agaccagggc cggcgaaaaa gacgaaattc tcctgacgga tatcaacctg gatcgcaacg
     8761 aagaggtacg acgctggtgg cccttcttgc gcgaccgccg catagacgcc tatcaaaatt
     8821 tactgaaacg ttttgtggat taattttttt gagcttattg accagaaaac gctttgacca
     8881 aaaa
//
LOCUS       DAOSTD010000073         8653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_7756_length_8653_cov_13.462898, whole genome shotgun sequence.
ACCESSION   DAOSTD010000073 DAOSTD010000000
VERSION     DAOSTD010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8653
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7756_length_8653_cov_13.462898"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PLI37_05800"
     CDS             complement(<1..574)
                     /locus_tag="PLI37_05800"
                     /inference="COORDINATES: protein motif:HMM:NF022187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOQ58555.1"
                     /translation="MKIGIIGVGNIGKAIVSGLAKSDFVPEENIAISDIEKSNLELIK
                     GSFPKIQSSTENKTAAQADIVIVAVKPWLVGEVLTEIAPLLETSRPILVVVAAGVSFE
                     QIYGKLPGSLSNLPCYRFIPNTAIALRESMSLLSAANTTPEQDKLMMGMLRKLGKAVL
                     IHPDMMAAGTSLTSCGTGFALQFIKANVQVA"
     gene            complement(695..1825)
                     /locus_tag="PLI37_05805"
     CDS             complement(695..1825)
                     /locus_tag="PLI37_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008024518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ58556.1"
                     /translation="MNLFDVYPLYDLEIVSGKNCYIYDKQGREYLDLYGGHAVISIGH
                     SHPHYVRSICEQVARIGFYSNSVVNSLQTKFATRLGKISGYEEYSLFLVNSGAEANEN
                     ALKLASFHNGRRKVVAFRKAFHGRTSAAVRVTDNPGIVAPVNQGMEVEFLPLNDIPAM
                     QNCLSRGDVCAVIVEGIMGVGGIHIPQQAFLQALRKTCNETKTVLILDEIQSGYGRSG
                     RFFAHQYADIRPDMITVAKGIANGFPMSGLLIGTEFKAVHGMLGTTFGGNHLACAAAL
                     AVLDVMEQENLIGNAARVGDFLMSECKTLSQIAEVRGLGLMIGLEFDKPIKELRKKLL
                     FEHAVFTGVSGSNVIRLLPPLSLNMEQAGVFIEKLRLALKTF"
     gene            complement(1946..2908)
                     /gene="argC"
                     /locus_tag="PLI37_05810"
     CDS             complement(1946..2908)
                     /gene="argC"
                     /locus_tag="PLI37_05810"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOQ58557.1"
                     /translation="MIKVGIVGGAGYTAGELLRVLINHPEVELVFAHSSSNAGKTVAS
                     VHGGLYGECDLVFTGDLTFDKIDVLFMCSAHGDSKKFLESHSLPPKLKIIDLSTDYRD
                     ESDGFVYGLPELQKEKIRRADKVANPGCFATCIELALLPLAAAGLLTSDIHVNAITGS
                     TGAGVKPSSTSHFSWRNNNVSVYKAFEHQHLKEIRQSLSALQKGFKAGIHFIPLRGNF
                     SRGILATAYTTCPWSLEEAIKAYTKFYREARFCFVCEENPDLKQVVNTNKCLLYLEKH
                     GDQLLIISVVDNLLKGASGQAVQNMNLMFGLDERAGLNLKSTGF"
     gene            complement(2905..4107)
                     /locus_tag="PLI37_05815"
     CDS             complement(2905..4107)
                     /locus_tag="PLI37_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOQ58558.1"
                     /translation="MKEKVVLAFSGGLDTSFCVKYLSIEKNFDVYTAVANTGGFSEED
                     LKVIEDKALRLGAVKHVSLDITQEYYEKSIKYMVFGNVLRNGTYPISVSSERIFQAMA
                     IIEYAKSVGAKYVAHGSTGAGNDQIRFDLTFQILAPEIKIITPTRDMVLTREYEIDYL
                     KKHGYQADFTKMEYSINKGLWGTSIGGKETLHSEQTLPESAYPSQITKEGSEVLCIDF
                     HKGEISGVNGVAYTDKVKAIQKVEEIASAYGIGRDMHIGDTIIGIKGRVGFEAAAPIL
                     IIQAHKMLEKHTLTKWQQYWKDQIGTWYGMFLHEAQYLEPVMRDMEAFLSSSQGNVCG
                     RVSIRLRPYSFSLIGVDSPYDLMKTDFGEYGEVNKAWDANDMKGFTKILGNQMKIYYN
                     VQDRVNKK"
     gene            complement(4165..4722)
                     /locus_tag="PLI37_05820"
     CDS             complement(4165..4722)
                     /locus_tag="PLI37_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ58559.1"
                     /translation="MDKKFEIVVANASHLQYVDIILNTIEEAAKIRGTGIAKRSPEYL
                     TQKILEGKAILALDGDIFAGFCYIETWGNKQFVANSGLIVVEQYRQLGLAKKIKKKAF
                     KLSRTKFPDAKLFGLTTSAAVMKINYELGYRPVTFAELTDDEAFWKGCQSCVNYPILA
                     EKNRRFCMCTAMLYDPKEHKRHRRI"
     gene            complement(4805..5278)
                     /gene="argR"
                     /locus_tag="PLI37_05825"
     CDS             complement(4805..5278)
                     /gene="argR"
                     /locus_tag="PLI37_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOQ58560.1"
                     /translation="MKNKANRMKSIRKIVHETRISSQEELLKLLTEEGFSLTQATLSR
                     DLKQLKIAKMPDYEGGYIYVLPEESSPLSRTVRMKSQDVHFIQSGFESLAFCGHFAVI
                     KTKPAYAGGIAAEIDKKGSKLILGTIAGDDTILVIPKEKTDRQSLMQALSEIIPL"
     assembly_gap    5464..5563
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5568..6971)
                     /locus_tag="PLI37_05830"
     CDS             complement(5568..6971)
                     /locus_tag="PLI37_05830"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HOQ58561.1"
                     /translation="MLFSSMSFIYLFLPVVCLLYFLVRANLRNYVLLGASIFFYAWGE
                     PEYLAIMLLTILVNYIGGLLIDKYPKYKKLSLTLTIAVDLGFLAYFKYFNFFIENINL
                     LLKGGGLNFIEVIMPIGISFYTFQAISYAVDVYRGEVKVQRDIGKLALFICLFPQLIA
                     GPILKYNDIAHQIDSREVNPEKVTLGVKRFIIGLSKKVLLANTLGAVADKIFELSPDL
                     VQTSIAWVGSLAYSFQLFFDFSGYSDMAIGLGLIFGFHIKENFNYPYISKSITEFWRR
                     WHISLSTWFKEYLYIPLGGNRVSKPRNYFNLGVVFLLTGFWHGAAWNFVVWGIWHGLF
                     IIFEKITGWHKESSRRWIDPVKHLYTIFVFIVGWVMFRAENLSYAWQYIKNMFGLTRN
                     KTVLYEAAYYFDNIEIIAFIAALLCSVPLFSNILNIPQERKWLRGLVNAWLLFLFILS
                     TSAIAASTYNPFIYFRF"
     gene            complement(6976..8151)
                     /locus_tag="PLI37_05835"
     CDS             complement(6976..8151)
                     /locus_tag="PLI37_05835"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HOQ58562.1"
                     /translation="MIALIIGFISFTSLYTLFSFLEKLRLSLPDLVLVVAFFALLPLP
                     AAKINKDKISVEENRTLAKFPALIVGKHVNNNFGTDFEAWFNDRFFMRKALVWLHNSI
                     KKEVNIHKQNDYILIGEDGWTFCKDDGDIQNYQNLKLFSPEELKHIALYLSSIDHWCN
                     ANNIKFYFLIAPDKNKIYGEYYKFARKIRPDSESRTQQLIRYLNENTRVKVAYPYESL
                     HAAKQNGFLLYFKNDIHWTHLGAYCGYTDLMELIGKDFEISPVRFEKTTTYKYPRGDA
                     NILFPEGVKEDTITLYHCPVLPINYTLTGEMETKKNLFYHNPKGRYSAVFFRDSFCTL
                     LADYYLGNTFEHLSLKWQYEITPKDLDFVIGKQADIVVLEQVENFIPKLLSIEILKD"
     gene            complement(8207..>8653)
                     /locus_tag="PLI37_05840"
     CDS             complement(8207..>8653)
                     /locus_tag="PLI37_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58563.1"
                     /translation="AHMPWKRIAFCSILTILILYGFRNFIIVHSVKPLRIELEVHADK
                     NVWLECFYTTQAEENFTAEKSVRYLFQPKDSGLISITLPVEHLHKIRLDVGEMPGKIR
                     LGTLRIPGHTTIGYEAGIRYYNIDNVLINRGGGRHIFTTGRPLHYF"
BASE COUNT         2271 a         1883 c         1964 g         2435 t
ORIGIN      
        1 tggccacctg aacgttggct ttgataaact gtaacgcaaa gcccgtcccg caagaggtca
       61 gcgaagtgcc cgccgccatc atatccgggt gaatgagcac tgcttttccc aattttctaa
      121 gcatgcccat catcagcttg tcttgttcgg gagtagtatt ggctgccgac aacagactca
      181 tgctttctct caaggctatg gccgtgtttg gaataaagcg gtaacagggc agattcgaaa
      241 gactgcccgg cagtttaccg tagatctgtt caaaacttac tccggcagcc acaacgacca
      301 gaatcggtct tgaggtctca agtaaagggg cgatttctgt caggacttcc cccaccagcc
      361 agggtttgac agccacaata acgatgtcag cctgagcggc tgttttgttt tcggttgaac
      421 tttgaatctt gggaaaagaa cctttaatca gttcgagatt cgatttttcg atgtccgaaa
      481 tggcgatgtt ctcttcgggt acaaagtccg atttggccaa gcctgacacg atggctttac
      541 cgatgttgcc tactcctata attccgattt tcataattga actttttaat gacagccgtt
      601 aagctaaaga gttatattta tcgaagcctt gacgaacgat ggtattcctc ggtgagagga
      661 tagatttcat tatcacagct ccttgttttg ttacttaaaa ggtttttaaa gccagtctga
      721 gtttctcgat aaaaacaccg gcctgctcca tgttcaggct caaagggggc aacaggcgaa
      781 taacgttgct tccgcttact cctgtaaaca cagcgtgctc aaagagcagt ttttttctga
      841 gttctttgat gggcttatcg aattccaggc ctatcatcag ccctaaaccg cgaacttccg
      901 ctatttgcga taaagttttg cattcactca tcaaaaagtc gcctactcgg gctgcgttgc
      961 cgatcagatt ttcctgttcc ataacgtcga gtactgccag ggccgcggca catgccaggt
     1021 ggttgcctcc gaaagtggtt cccagcatgc cgtgaaccgc cttaaattcg gtgccgataa
     1081 ggagtccgct catgggaaaa ccgttggcga tgcctttggc tacggtgatc atatcgggcc
     1141 gtatatcggc gtattgatga gcaaaaaacc ggccgctccg cccgtagccc gattgaattt
     1201 cgtcgagaat caagacggtt tttgtctcgt tacaggtttt gcgcaaagcc tgcaaaaagg
     1261 cctgctgggg aatatgaata ccgcctacgc ccataatgcc ttctacaatg acagcgcaga
     1321 catctcccct cgataagcaa ttttgcatgg ccggtatgtc gttgagcggc aggaattcca
     1381 cttccatgcc ctggttgacc ggagccacta tgccggggtt gtcggtgacg cgaacagccg
     1441 ccgaagtacg accgtgaaaa gctttgcgaa aggccactac tttgcggcgt ccgttatgaa
     1501 acgaagccag tttgagggca ttttcgttgg cttctgcccc ggaattcacc aaaaagaggc
     1561 tgtactcttc gtagccggaa atctttccca aacgggtggc aaacttggtt tgcaggctgt
     1621 ttactacgga attggaataa aaacctatgc gagcgacttg ttcgcagatg ctccgtacat
     1681 aatgcgggtg tgaatgcccg atggaaatca cggcgtgacc tccgtacagg tctaagtatt
     1741 ctcttccttg tttatcgtaa atgtagcagt tcttgccact gacaatttcg aggtcgtata
     1801 ggggataaac atcaaataaa ttcattttct tcaagttttg ttataagcaa agcgaatttt
     1861 ggccaggcga attaaaataa aagatttaca gcaaatcaaa aatccatcca aaataaggcc
     1921 tcagcaaagg ccttcgcata aaggattaga accccgtact cttaagattc aagcctgccc
     1981 tttcgtccag gccaaacatc agattcatat tttgaaccgc ttgcccggaa gcgcctttta
     2041 ataaattgtc caccacagag ataatcaaaa gttgatctcc gtgcttttcg agatacaaca
     2101 gacatttgtt ggtgttgacc acttgcttga ggtcgggatt ttcttcgcag acgaaacaga
     2161 aacgagcctc gcggtaaaac ttggtatagg cttttatcgc ctcttcgagg ctccaggggc
     2221 aggtggtgta ggccgttgcc agaatgcctc tggaaaaatt accacgcaga ggaatgaaat
     2281 ggatgccggc tttgaatcct ttttgcaagg cgctcagcga ttgcctgatt tctttgaggt
     2341 gctgatgttc aaaagcttta taaaccgaaa cattgttgtt cctccaactg aaatgtgagg
     2401 tggacgaggg ttttacgccg gcgccggtgg agccggtaat ggcattgacg tgtatatccg
     2461 aagtcaacag accggcagcc gccaggggaa gcagggccag ttcaatgcag gtggcaaagc
     2521 agcccggatt ggcaactttg tctgccctac gaatcttctc tttttgtaat tcgggcaaac
     2581 cgtaaacaaa accatcgctt tcgtcgcgat aatcggtgga taagtcgatt attttgagtt
     2641 tgggcggaag agaatgactt tccagaaatt tcttgctgtc gccgtgagcg gagcacataa
     2701 acaagacatc gatcttgtcg aaagtcaaat caccggtaaa aacaagatca cattctccgt
     2761 aaagtcctcc gtgtaccgag gcaacagttt tgccggcgtt gctgctgctg tgtgcaaaca
     2821 caagctcaac ttcggggtga tttatcaata cccgcagcaa ttccccggcg gtgtagcccg
     2881 ctccgccaac aattcctact ttgatcattt tttgtttact ctgtcttgta cgttgtaata
     2941 aattttcatc tggttgccca gtatcttggt aaagcctttc atatcgttgg catcccaggc
     3001 tttgtttact tcgccgtatt caccgaaatc ggttttcatc agatcgtaag gactgtctac
     3061 tccaatcagg ctgaagctgt agggtctgag cctgatgctg accctgccgc agacattgcc
     3121 ctggctgctg ctgaggaaag cttccatgtc gcgcatcacc ggttccaggt attgtgcttc
     3181 gtgcaggaac attccgtacc aggtgccgat ctggtctttc cagtattgtt gccatttggt
     3241 cagggtatgc ttttcgagca ttttgtgcgc ctgaatgatg agtatgggtg cggcggcttc
     3301 gaaaccaacc cgtcccttga tgcctataat ggtgtcgccg atgtgcatgt cgcgtcctat
     3361 gccgtaggct gaggcaattt cttctacttt ttgtatggct ttcactttgt cggtataggc
     3421 gacaccgttc actcccgaga tttcgccttt atggaaatcg atacaaagca cttcgctgcc
     3481 ctctttggtt atttggctgg gataggccga ttcgggcagt gtctgttcgg aatgaagggt
     3541 ttccttgccg cctatggagg ttccccaaag ccctttgttg atggaatatt ccattttggt
     3601 aaagtcggcc tggtaaccgt gtttttttag gtaatcgatt tcatattcgc gagtcaacac
     3661 catgtcgcgg gtgggggtga ttattttgat ttcgggagcc agtatctgaa aagtcagatc
     3721 gaagcgtatc tgatcgttgc cggcccccgt actgccgtga gcaacgtatt tggcgccgac
     3781 ggatttggcg tattcaataa tggccatggc ctgaaatatg cgctccgaac ttaccgagat
     3841 gggataagtg ccgttgcgta agacattgcc aaacaccata tacttaatgc ttttttcgta
     3901 atattcctgc gtgatgtcga ggcttacgtg ctttacggct cccagacgga gggccttatc
     3961 ttcgattacc ttgaggtctt cctccgaaaa gcctcctgtg ttggcaacgg cggtatagac
     4021 atcgaaattc ttttcgatgc taaggtattt tacgcagaag gaggtatcca atcctccact
     4081 aaaggctaaa actacttttt ctttcatctt attatgctgt tttttagatt gtcgtttgaa
     4141 ttgaatgttt ttaagtgtgc ctgtttaaat gcgccggtgt cttttatgtt ctttgggatc
     4201 gtacaacatg gccgtgcaca tgcagaagcg acggtttttt tcggccagaa tcgggtagtt
     4261 tacgcaactc tggcagcctt tccaaaaggc ttcgtcgtcg gttaattctg cgaaggtaac
     4321 cggtctgtag ccgagttcgt aattgatctt cattacggcc gcgctggtgg tcagtccaaa
     4381 caattttgcg tccggaaact tggtgcgcga cagcttaaag gcttttttct tgattttttt
     4441 ggccaggcca agttgcctgt attgttctac cactatcaga ccggaattgg ctacaaactg
     4501 tttgttgccc caggtctcga tgtagcaaaa gccggcaaag atgtcgccgt ccagagctaa
     4561 tatggctttt ccttcgagta ttttttgagt caggtattcc ggagaacgtt tggcgatacc
     4621 ggttcctctt attttggcgg cttcttcgat agtgttcagt ataatgtcaa cgtactgtaa
     4681 atgcgatgcg tttgcaacta cgatctcaaa ttttttatcc atttcaatct ttaaacaaac
     4741 ctgttcagat aaaatttttc gcatttagaa cagggtaaaa ttctttaata gggtgaattg
     4801 taaatcaaag tggaataatc tccgataagg cttgcataag gctttgtcga tcggtctttt
     4861 ctttggggat taccaaaatg gtgtcgtctc ctgcgatggt tcccaaaatg agtttcgagc
     4921 cttttttgtc gatttcggcg gcaattcctc cggcgtaggc aggcttggtt ttgattacgg
     4981 caaaatgtcc gcaaaaagca agcgattcaa agccggattg aataaaatgt acgtcttgcg
     5041 atttcatacg tacagttcta cttaaggggg aagattcttc gggcaaaacg taaatatatc
     5101 caccttcgta atcgggcatt ttggcaattt tgagttgttt gagatctctc gacaaagtgg
     5161 cctgcgtaag gctgaagccc tcttcagtaa gtagcttcag taattcctcc tggctgctga
     5221 tgcgcgtttc gtgtacgatt tttcgtatgc ttttcatacg attggccttg ttcttcattt
     5281 ttcctctttt gaataaaaat acaaaaacaa gccgcaaaat tatacattat ttttatggcc
     5341 gcaaaataat tatataaata aaccgataaa aattgcgaga gttccaaatg ccgggcctaa
     5401 acgactcctc cgtcagaaac ggaaataaat gaaaggattg taagtggagg cggcaattgc
     5461 cgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntccgtca gaaacggaaa
     5581 taaatgaaag gattgtaagt ggaggcggca attgccgaag tggagaggat aaacaaaaac
     5641 agcaaccagg catttaccag tcccctgagc cattttcgtt cctgaggtat gttcaatata
     5701 ttcgaaaaca aaggaacgga acagagtaag gccgcaataa aggcgattat ctcgatgtta
     5761 tcaaagtaat aggctgcttc gtataagacg gttttattcc gggtcagtcc gaacatgttt
     5821 ttgatgtatt gccaggcata gctcaggttt tcggctcgga acataaccca gccaacgata
     5881 aagacaaaaa tggtatagag gtgtttaacc ggatcaatcc atcgtcttga actttctttg
     5941 tgccagccgg tgattttttc gaaaataata aaaaggccgt gccagatgcc ccagaccaca
     6001 aaattccagg cagccccatg ccaaaacccc gttaaaagaa atactacgcc caggttgaag
     6061 taattccggg gcttactcac ccggtttcct ccgagaggaa tataaaggta ttctttgaac
     6121 caggtggaca gggatatatg ccatctgcgc caaaattcag taatggattt tgaaatatag
     6181 gggtaattga aattttcttt aatgtgaaaa ccaaaaatca agcccaggcc aatggccatg
     6241 tccgaatatc cgctaaaatc gaaaaataat tgaaagctat aggccaggct gccgacccag
     6301 gcaatggaag tctgtacaag atcgggcgac agttcgaaaa ttttatcggc aaccgcgccc
     6361 agggtattag ccagcaatac ttttttagaa agccctataa taaaacgctt cacacccaaa
     6421 gttacttttt cggggttgac ttcgcgggaa tcgatctgat gggcaatgtc gttgtatttt
     6481 aaaatagggc cggcaatcaa ttgggggaaa agacaaataa acaaagccag cttgccaata
     6541 tcccgttgca cctttacctc acctctgtaa acatctaccg cataagaaat ggcctgaaaa
     6601 gtataaaacg aaatacctat gggcataatg acttctataa aattcagtcc tccccctttg
     6661 agtaataaat tgatgttttc gatgaagaaa ttaaagtatt taaagtaggc caaaaagccc
     6721 agatcaaccg ctatcgtaag cgtaaggctc agctttttat acttcgggta tttgtcaatt
     6781 aaaaggcctc caatgtaatt aaccaaaata gtcagcaaca taatggctaa atattccggt
     6841 tctccccagg cataaaaaaa gatactggct ccgagcaata cataattgcg caaattggct
     6901 ctgaccagga aatacagcag gcagactaca ggtaaaaaca agtaaataaa actcatcgaa
     6961 gagaagagca tggcatcagt cttttaagat ttcgatactc aataatttgg gaataaagtt
     7021 ttccacttgt tccagcacca caatatctgc ctgtttaccg ataacaaaat ccaggtcttt
     7081 tggtgtgatc tcgtattgcc atttcaagct gagatgttca aaggtgttgc ctaaataata
     7141 atccgccagc agggtgcaaa aggaatcccg gaaaaatacg gcagaatagc gacccttggg
     7201 attgtggtaa aacaggttct ttttggtttc catctcaccc gtaagagtat aattaatggg
     7261 caaaaccgga caatgataaa gtgtgatggt atcttcttta accccttcgg ggaataaaat
     7321 attcgcatct ccccggggat atttataggt ggtggttttt tcgaatctta cagggctgat
     7381 ttcgaaatcc ttgccaatca gttccatcaa atcggtataa ccgcaatagg ctcccaggtg
     7441 ggtccaatga atgtcgtttt taaaatacaa cagaaagccg ttttgtttcg ctgcatggag
     7501 gctttcgtag ggataggcca ccttgacccg ggtgttttcg ttcaggtagc gaatcagttg
     7561 ttgagtacga ctctcggaat cggggcggat ttttcgtgca aatttgtaat actctccgta
     7621 aatcttgttt ttatcgggcg caatcaggaa gtaaaacttt atattattgg cgttgcacca
     7681 atgatctata gaggataaat acagagcgat atgctttaat tcttccggac taaagagttt
     7741 aagattctga taattctgaa tatcgccatc gtccttacag aaagtccagc cgtcttcccc
     7801 tataagaata taatcgtttt gtttgtgaat attcacttct ttcttgatgg aattgtgcag
     7861 ccagaccagt gcttttctca taaagaagcg atcgttgaac caggcttcga agtcggtgcc
     7921 gaaattgttg tttacatgct ttcctacgat taatgcggga aattttgcca aggtcctgtt
     7981 ttcttcgaca gatattttgt ctttgttgat tttggcggca ggaaggggca gaagtgcaaa
     8041 aaacgcaacc accagaacca gatccggaag actgaggcga agtttttcca gaaaggaaaa
     8101 taatgtataa agcgaggtaa aagaaataaa accgataatc agggcaatca acaggccgct
     8161 tttattcagg ctcaggcttt gtttagcgct aatattcagg ggttcattaa aaataatgta
     8221 ggggtcttcc tgttgtgaat atatgtctac ccccccccct gttaattagc acattatcaa
     8281 tgttataata ccttattcct gcctcgtatc ctatggtggt atgccccgga attctaagag
     8341 tacccagccg gattttgccg ggcatttccc ctacatccaa ccggatctta tgtaaatgtt
     8401 ctaccggtaa agtaatgctg ataagccctg agtctttggg ctgaaacaag tatcgtacgg
     8461 atttttcggc ggtaaagttt tcttcggcct gagtggtata aaagcattcc aaccaaacgt
     8521 ttttatcggc atgtacttcc agttctattc ggagaggttt aaccgaatga acgataatga
     8581 aattccggaa accataaagg ataagaatag tcagaatgga gcaaaatgcg attcttttcc
     8641 aaggcatgtg cgc
//
LOCUS       DAOSTD010000074         8437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_7975_length_8437_cov_17.389644, whole genome shotgun sequence.
ACCESSION   DAOSTD010000074 DAOSTD010000000
VERSION     DAOSTD010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7975_length_8437_cov_17.389644"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="PLI37_05845"
     CDS             complement(<1..1514)
                     /locus_tag="PLI37_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HOQ58564.1"
                     /translation="MKKVFSTLLFIPLFSLVGFARFGEQWGDQGDGTYVNPVIPADIS
                     DIDAIRVGDDFYAISSTMQMSPGMLVLHSKDLVNWEMIGHVVNDLATISPELNWDRMN
                     CYGKGIWAGSIRYYKDKFWVYFGTPDDGLFMSSATDPAGPWEPLHQLWKVSGWNDCCS
                     FCDDDGQLYLVTTNFGYDPKNGKHYNIHLFKMTPDGKNLIMESDTIIYQASGSEANKL
                     YKIDGVYYHFFSEVNHEGRVTMMQRSRNIYGPYEVKQLNHVNSSKDREPNQGGLIGLD
                     DGSWWFFTHHGTGAWEGRVASLLPVHWVEGWPIIGQVGQDTIGNMVWTAEKPNLPSHN
                     KTKIQTDDDFSGKKLLPQWEWNYQPRADKWSLTERKGFLRLYAYRPIRPEDNTNILLR
                     AGNTITQRSIRSKANVVTVKMDISKTADGQCAGLTHFSTTSHSTLGIKQEKGIKNLVY
                     TVNGKDSIVSPVEQKNIWLQSSWDVRGVNTYSYSLDGKNFTPLNVFTRLTWGSYR"
     gene            complement(1526..3742)
                     /locus_tag="PLI37_05850"
     CDS             complement(1526..3742)
                     /locus_tag="PLI37_05850"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOQ58565.1"
                     /translation="MKKLLIPVLLLVFCTTNAQTVKIKIIDNGGSGQYEAVAAKDSTL
                     PDFVVYRPKDINKAVKKEGKLPVMVWANGGCMNSSIHQERFLSEIASHGYIVVAIGAL
                     QMTVGERPHRGTPDDELLKALDWISERATTKGNDYYKRVDLTKIAAGGHSCGGAQTLR
                     VADDPRIKTYLMLNAGMGDMTMAGASSKSLENLHGPIVYMIGGESDVAYKNALLDYDR
                     IDRVPVVFANHATAGHGATFAEEFGGSFAQMTLHWLDWQFKGKDNSGIFLENKLSAYP
                     AWTMKSKGFDTQAEDVKSQPVNPFGNALVPDMIADASIQEIDGVFYCYATTDGYGRGL
                     ETSGPPVVWKSKDFVHWSFDGTHFPSAMGQKYWAPSKAIAANGKYYIYPTVNEYMYAA
                     VADSPEGPFKLAAGPDTFIKPYSAYTLLKSEAPRRPAGIDAEVFIDDDQQAYLFWQSR
                     RAAKLGPDMVTVDTNIITIPTPRTEYSEGPIFFKRKGIYYYLYTIGGDEKYTYSYVTS
                     KVSPLGPFDFPPKDQDVVCITNYERQIFGPGHGCVFNVPGTDDYYLAYLEFGRGSTNR
                     QTYVNKLEFNADGSIRPVDLTMTGVGALRNIKTDPKKLVKKVEASSVRADLKIRPQQD
                     FTLNRVESFVAWFAFDGANGSRWMSAPEDTACWLVADLGKKQTIKRSEVYFVRPTAGH
                     SYLLEYSNDGTSWATCGGHSDLIMKSPHTDHLNIKARYLRIKITGGVKGVWEWNMY"
     gene            complement(3841..5355)
                     /locus_tag="PLI37_05855"
     CDS             complement(3841..5355)
                     /locus_tag="PLI37_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOQ58566.1"
                     /translation="MTISTELILLVGSVLFFISMLVGQAGHRFGVPILLLFLMVGMIF
                     GSDGFGLKFENIKMAQAIGSVCLAIILFSGGLDTRLSEIKPVIGPGVVLATLGVLLTA
                     IFTGLFTYWMTDRLFPALGLGFLSCMLLASTVSSTDSASVFAILRSKGLTLKNNLRPL
                     LELESGSNDPMAYLLTVMFIDIIQYGENPDYLKVGLSVLIQLVVGTAMGILLGRLAVK
                     IINKVKMDNDSFYPIMLLVFGVFIFASTYFLEGNGYLAVYIAGLVIGNSRFAHKRSSM
                     RFMDGFAWMSQILLFLTLGLLVNPSELVEVIVPALLISFFMIVFARPITVFLCLIPFR
                     KVGFKDKLYVSWVGLRGAVPIIFAILPLAAGVPGARLIFNIVFVLTLVSLLMQGTTLP
                     IVARMFGLAEKPKQYRPLKDFDVDFSEEIKSVMTEILITEETLSYGENLMEMPLPDNT
                     LVVMVKRGDNYFIPRGQTELHVNDILLVISDDEEALKESYSNIGISDFTYRKNQ"
     gene            complement(5629..6447)
                     /locus_tag="PLI37_05860"
     CDS             complement(5629..6447)
                     /locus_tag="PLI37_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58567.1"
                     /translation="MTKTKVLITVKTYPTISKKYEELVCTAGFLEDGSWIRIYPIQFR
                     KKSYSEQYSKYDWIELDLVKNKSDFRPESFRPISHDTVIEKVGEIEADGDTWDERRKI
                     VLRNVYHNISDLISEAKNKKTCTSLAVFKPTEVLDFIYEEEKVRVWSSDKLAQFQQLN
                     IFERVKSNNFQVVRKLPYKFSYCFKDEAGTVCKLMIEDWETGQLYWNCLSRHDGDEKK
                     ACEDVRKKYFDDFVKTKDLHFFLGTTQVHHFRAKNPFVIIGTFHPKPITQLKLF"
     gene            complement(6447..7331)
                     /locus_tag="PLI37_05865"
     CDS             complement(6447..7331)
                     /locus_tag="PLI37_05865"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOQ58568.1"
                     /translation="MYYRRKILLSLLQAFDNQLDKISLQKLLFLLTRIQEKKSYYFIP
                     YKFGCFSFQANADLSTLAKYKLVEPSMKYWKKTDNTDYIEELDSKDKAAIRFLKSQYG
                     NLSKDELIALTYRQYPFFAINSIIASSILTNEELNKVKNQRPIKNTIVLFTIGYEGIS
                     LEQYINKLIVNDIRVLCDVRKNSYSMKYGFSKNQLKKACGGVGIDYVHIPEVGIESKK
                     REVLNSQSDYDKLFEYYKSDVLSREKSKQVEIFSLLKSKKRIALTCFEANINQCHRKH
                     LAESISSLNGFSYELIHI"
     gene            complement(7528..8058)
                     /locus_tag="PLI37_05870"
     CDS             complement(7528..8058)
                     /locus_tag="PLI37_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOQ58569.1"
                     /translation="MIKLIRGDITKIKVDAIVNAANTSLLGGGGVDGAIHRAGGKTIL
                     EQCIKIREKQGGCKVGEAVLTGAGELPAKYVIHTVGPIWRGGNQNEDRLLSNAYRNSL
                     QLAAENKVESISFPNISTGVYHFPKERAANIALTTVMDYLKSNPTIKEVIFVCFDQQN
                     YDIYDRLLNRTEGKLN"
     gene            complement(8105..>8437)
                     /locus_tag="PLI37_05875"
     CDS             complement(8105..>8437)
                     /locus_tag="PLI37_05875"
                     /inference="COORDINATES: protein motif:HMM:NF028226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HOQ58570.1"
                     /translation="KIIEDNKPDEWIDIHNFKLIKYGNAYHINCDLVLPWNLYLSEAH
                     EEGEKLKKLLVSNFREDIVFNVHTDECFKKYCKHCKRKDCKERTTGFVTRLNFDLERF
                     TKEKIEKS"
BASE COUNT         2270 a         1942 c         1717 g         2508 t
ORIGIN      
        1 cggtagctgc cccaggtgag tctagtgaat acattcagcg gagtaaaatt tttcccgtcc
       61 aaactgtacg aataggtatt cactcccctt acatcccagg acgattgcag ccaaatgttt
      121 ttttgctcaa cgggagacac gattgaatct tttccgttaa cggtgtacac caggtttttt
      181 atcccttttt cctgttttat ccccagcgtg ctgtggcttg tggtcgagaa atgcgttaat
      241 ccggcacatt gaccgtcggc cgttttgctt atatccattt taaccgttac cacattggct
      301 tttgagcgta tactgcgttg ggtaatggtg ttaccggcgc gcagaagtat gttggtgtta
      361 tcttcgggcc ggatcggacg gtaggcataa agccgcaaga agccttttct ttcggtcagc
      421 gaccacttat cggcccgtgg ctggtagttc cattcccatt ggggcaacag ctttttgccc
      481 gaaaaatcat cgtctgtttg aatcttcgtt ttattatgac ttggcaggtt gggtttctcg
      541 gcagtccata ccatattgcc aatggtgtct tgcccgactt ggccgatgat cggccagcct
      601 tccacccaat ggacgggcaa taaactggca accctgcctt cccaggcgcc tgtgccgtgg
      661 tgcgtgaaaa accaccaact accgtcgtcc aaacctatca atccgccctg attgggctcc
      721 ctgtctttgg aactgtttac gtggttcaat tgttttactt cgtaaggccc gtaaatattt
      781 ctcgagcgtt gcatcatcgt aacccggccc tcgtggttta cttcgctaaa gaaatgataa
      841 tataccccgt cgattttgta caatttgttc gcctcgctcc cgcttgcctg gtagatgatg
      901 gtatcggatt ccataatcag gtttttaccg tcgggcgtca ttttgaaaag gtgaatattg
      961 tagtgtttac cgtttttggg gtcgtaacca aagttggtgg taaccaaata aagttgtccg
     1021 tcgtcgtcgc aaaacgaaca gcaatcgttc cagccactca ctttccatag ttggtgcaat
     1081 ggttcccacg gaccggccgg atcggtggcc gaactcataa agaggccgtc gtcgggcgta
     1141 ccgaaataaa cccaaaactt atctttataa taacggatgg aaccggccca gatacctttg
     1201 ccgtagcaat tcatcctgtc ccagttgagt tcggggctga tggtggcaag gtcgtttacc
     1261 acatgaccga tcatttccca attcaccagg tctttggaat gaagcaccaa catacccggc
     1321 gacatctgca tggtggacga aatggcgtag aaatcatcgc ccacccgaat ggcgtcgata
     1381 tcgcttatat cggccggaat aaccgggtta acgtaggttc cgtctccctg atcgccccat
     1441 tgctcgccga agcgggcaaa gccaacgagg ctgaaaagcg gaataaacaa cagagttgaa
     1501 aaaacttttt tcatcttgcc ggttcttaat acatattcca ttcccaaact cctttaacgc
     1561 ccccggtaat tttgattcgt aaatagcgtg ccttgatatt caagtggtcc gtatgcggcg
     1621 atttcattat aagatcgctg tgccccccgc aggttgccca cgaagttccg tcgttggagt
     1681 attcgagcag ataggaatgt ccggcggtcg gtcttacaaa atacacctca cttcgcttta
     1741 ttgtttgttt tttgcccaaa tccgccacaa gccaacaggc cgtatcttcg ggagcagaca
     1801 tccaacgcga accgttggcg ccgtcgaatg caaaccaagc cacaaacgac tccaccctgt
     1861 tcaatgtaaa atcttgttgc ggtctaatct ttaaatcggc acgaacactc gatgcctcaa
     1921 ccttttttac aagcttcttc ggatcggttt taatgttgcg caatgcgccc actccggtca
     1981 tggtgagatc aaccggacgg atagaaccgt ctgcattgaa ctccagctta tttacatagg
     2041 tttgacgatt ggtgctgccc cgcccgaatt ccaggtaagc aagataataa tcatcggttc
     2101 cgggcacatt aaaaacacaa ccgtggccgg gaccgaaaat ctgccgctcg taattggtta
     2161 tgcaaacaac atcctggtct ttgggtggaa aatcaaaggg tcccaatggc gaaactttac
     2221 tcgtaacgta agaataagtg tatttttcgt cgcctccgat ggtgtataaa taatagtaaa
     2281 tgcctttgcg tttaaaaaag atcgggccct cggagtattc cgtgcgcggg gtgggaatgg
     2341 tgatgatgtt tgtatcgacc gtaaccatgt ccgggccgag tttagccgct ctgcgacttt
     2401 gccagaagag ataagcctgc tgatcgtcgt cgataaaaac ttcggcatcg atgcccgccg
     2461 gtctcctcgg cgcttccgac ttcaacagcg tatatgccga ataaggctta ataaaagtgt
     2521 cggggccggc tgccaattta aaagggccct ccggcgagtc ggcaacggca gcgtacatat
     2581 attcgtttac cgtgggataa atatagtatt tgccgttggc cgcaatggct ttactcggcg
     2641 cccagtattt ctgacccatg gcagagggga aatgggttcc atcgaaactc cagtgtacaa
     2701 aatctttcga tttccagaca acgggcggac cggaagtttc aagtcccctg ccgtatccgt
     2761 cggtggtggc gtaacaataa aacactccgt ctatttcctg gatactggca tcggcaatca
     2821 tatcgggaac cagtgcattg ccaaaaggat taacaggctg agattttaca tcttccgctt
     2881 gcgtatcaaa gcctttggat ttcattgtcc aagccgggta agccgacaac ttgttttcaa
     2941 gaaaaatgcc ggagttgtct tttcctttaa actgccagtc gagccaatga agcgtcattt
     3001 gagcaaacga gccgccaaac tcttctgcaa aggtggcacc atgcccggct gtggcgtgat
     3061 tggcgaaaac aacgggaaca cgatcgattc gatcgtaatc taaaagggcg tttttgtagg
     3121 caacatccga ttctcctcca atcatataga cgattgggcc atgtaagttt tccagcgatt
     3181 tcgagctggc tcccgccatg gtcatatccc ccatgccggc gttcagcatc aggtaggttt
     3241 ttatccttgg gtcgtctgcg acacgaaggg tttgtgctcc accgcatgaa tgtccgcctg
     3301 ctgctatttt agtgagatca acacgcttgt aataatcgtt tcctttcgtc gtcgccctct
     3361 ccgaaatcca atcgagggct tttagcagtt cgtcgtcggg tgtacctcta tgcggcctct
     3421 ctcccacagt catttgcaat gccccgatgg ccactacgat atagccatgc gaagcaatct
     3481 ccgacaaaaa acgctcctga tggatagacg aattcataca gccgccgttc gcccacacca
     3541 ttacaggcaa tttgccctct ttttttacgg ctttattaat gtctttaggg cgataaacca
     3601 caaaatcggg gagagtactg tctttcgccg caacggcttc gtattgtccc gatccgccat
     3661 tgtcgataat cttgattttt accgtttgtg cattggttgt acaaaaaacc aataaaagca
     3721 cgggaattag taattttttc atggctttat gtttaattat tagacaattt tgttgatctg
     3781 tgtattgtcc ataatgaatg gcaataacca ctttgactat tttcttcctt tattcccgtt
     3841 tcactgattt ttacgatacg taaaatctga aattccgatg ttggaataac tctctttcaa
     3901 ggcctcctca tcgtcggaaa tcaccagcaa tatatcgttg acgtgcagtt cggtttgccc
     3961 tctcggaata aagtagttgt cgcccctttt taccataacg accagggtgt tgtcgggcaa
     4021 aggcatctcc atcaggtttt caccgtaact caatgtctct tcggtaatca ggatctccgt
     4081 cattaccgat tttatttctt ccgaaaagtc aacgtcaaaa tcttttaaag gtctgtattg
     4141 cttcggtttt tcagccaagc cgaacatgcg tgccacaatg ggcaaagtgg ttccttgcat
     4201 caacaacgag acaagggtaa gcacaaacac aatgttgaat atcaatcgcg caccgggcac
     4261 tccggcagcc agaggtaaaa tggcgaaaat aatgggaacc gcaccgcgca atcccaccca
     4321 cgacacatag agtttgtctt taaatcccac ctttcggaaa ggaatcagac ataagaaaac
     4381 cgtaatgggg cgcgcgaata caatcataaa gaaagaaata agaagcgcgg gaacaattac
     4441 ctcgaccagc tcactgggat taaccaacaa accgagcgta aggaacaaca atatctgact
     4501 catccacgcg aatccatcca taaacctcat ggaagagcgc ttgtgcgcaa accgggagtt
     4561 tccgattacc aggccggcga tgtaaaccgc caggtagccg tttccttcca ggaagtaggt
     4621 ggaagcgaaa ataaataccc cgaaaacaag gagcataatt ggataaaacg aatcgttatc
     4681 cattttaact ttgttgataa ttttaaccgc taaacgcccc agtagtatcc ccattgcagt
     4741 acccacaacc aactggatca acacactaag tcccaccttc aggtagtcgg gattttcgcc
     4801 gtattgaata atgtcgataa acatcaccgt aagcaaatag gccatcgggt cgttgctacc
     4861 gctctccagt tcaagtaaag gacgcaggtt gtttttcaag gttaagcctt tcgaacgcaa
     4921 aatggcgaaa acggaagccg aatcggtcga cgaaaccgtc gaagcgagca gcatacatga
     4981 aagaaagccc agtcccaagg cagggaaaag acggtcggtc atccaatagg taaacaatcc
     5041 ggtaaaaata gccgtcagca acactcccag ggtagcaagt actactccgg ggccgataac
     5101 gggttttatc tccgacaacc gggtatccaa acctcccgaa aacaaaataa tagccagaca
     5161 aaccgagcct atggcttgtg ccatttttat attttcaaat ttaagcccga atccatcgga
     5221 accgaagatc atgcccacca taaggaaaag caataaaata ggcaccccaa agcggtggcc
     5281 cgcctgccct accagcatac tgataaaaaa cagtaccgag ccgacaagca atattagttc
     5341 tgttgaaata gtcatctgta attgttcaaa tttagctgaa tcctcttatt gcatttattg
     5401 gcgcacgtgc tcccacacta acgcccggat ttttatttgc agggcaaaga tacaacattt
     5461 ggaaataaaa aatatagggt gcgtccctga taacgatcat gcatacagat aagataagta
     5521 cctatgccgt ttgggacgaa ctggaaggct attgggcaaa attggattgc agcgtggctt
     5581 gtgcgtcttg ccaaagtgcc tgacacgagg agaagatttt gaattgactt agaataactt
     5641 taattgagtt atcggttttg ggtggaatgt tccaataatt acaaatggat ttttagctct
     5701 aaaatgatga acttgagttg tacctaaaaa gaaatgtaag tctttagttt taacaaaatc
     5761 atcgaaatat tttttcctta cgtcttcaca tgcctttttc tcatctccat catgtcgact
     5821 taaacaattc caataaagtt gtccagtctc ccagtcttca atcataagtt tacaaacagt
     5881 acctgcttca tccttaaaac aataactgaa tttataaggt aatttcctaa caacctgaaa
     5941 gttattgctt ttaactctct cgaatatatt taactgttga aattgtgcca atttgtctga
     6001 tgaccaaacc cttacttttt cctcttcata aataaagtct aaaacttctg ttggcttgaa
     6061 tactgccaat gaagtgcaag tctttttgtt ttttgcttcc gaaattaggt cggaaatgtt
     6121 atgatagacg tttcttaaaa ctatttttct tctttcgtcc caagtatcac catctgcttc
     6181 gatttcaccg actttctcta tcacagtatc atgagaaatg gggcgaaaac tttcgggacg
     6241 gaaatcgctt ttatttttaa ctaaatccaa ttcaatccag tcatatttag agtattgttc
     6301 actgtacgat tttttacgaa attgaatagg ataaattcta atccatgaac catcttctaa
     6361 aaatcctgca gtgcaaacta attcttcata cttttttgag attgtaggat aggtttttac
     6421 ggtaataaga acttttgttt tggtcatcag atatgtatta gttcatagga aaaaccattt
     6481 aaagaagata tcgattcggc taaatgcttt ctatggcatt ggttaatgtt agcctcaaaa
     6541 caagtgagtg ctattcgttt tttacttttt aacaacgaaa aaatttccac ctgctttgat
     6601 ttttctcttg atagaacgtc agatttgtaa tattcaaaaa gtttgtcata atcagactgg
     6661 ctgtttagta cttctctttt ttttgattca atcccaacct cagggatatg cacataatca
     6721 attccaacac ctccacaagc tttcttcagc tggttcttac taaaaccgta tttcatgctg
     6781 tacgaatttt ttcgcacatc acacaaaacc cttatatcgt taacaattaa cttgttaatg
     6841 tactgttcaa gcgaaattcc ttcataacca attgtgaata aaacgatagt attttttatc
     6901 ggcctttggt tttttacttt gtttaattcc tcgttggtca gaatgctact cgcaatgata
     6961 ctattaattg caaaaaacgg atattgacga taggttaaag cgattagttc gtctttactc
     7021 aaatttccat actgactttt taaaaatcga attgcagctt tatctttgga atctagttct
     7081 tctatgtagt ctgtattatc tgtttttttc caatatttca ttgatggttc taccaatttg
     7141 tatttagcca aagtacttaa atcggcattg gcttggaatg agaaacatcc aaatttatac
     7201 ggaataaaat agtacgattt cttctcctgt atccttgtta gtaagaataa cagtttttgc
     7261 agactaattt tatctaattg attatcaaaa gcctgaagca gggatagtaa tatttttcta
     7321 cgataataca taaggcaaag ctacaatata aaatggtttc ttccatcata attgcttctt
     7381 tctctttgct aaaaatttcg cttgactgct actaacacta tgtatttgtt tgtcaaataa
     7441 taacatacag ttaaataatc gggaacagtg ggggcttcat tagtcttttc agtcaatatg
     7501 cagcaaaggt gtcttaacgg ctggtgttca attcaatttc ccctctgttc tattcaatag
     7561 cctgtcgtaa atatcgtaat tctgctggtc aaagcataca aaaattacct ccttgattgt
     7621 agggtttgat tttaaataat ccataacggt ggtcagtgca atgttggctg ctctctcttt
     7681 tgggaaatga tagactcctg tgctgatgtt gggaaacgaa atcgattcta ctttgttctc
     7741 tgccgcaagt tgcaagctgt tccggtaggc attcgacaaa agccggtctt cgttttgatt
     7801 gccgccgcgc caaataggtc cgacggtgtg aattacatat tttgcaggca attctcctgc
     7861 cccggtcagc acggcttctc caaccttgca gccgccctgc ttttctctga ttttaatgca
     7921 ttgttcgaga attgttttac cgcctgctct gtgaatggct ccatcgacac cgccgccacc
     7981 gagcaatgag gtgtttgcgg cattcacaat ggcgtcgact tttattttag tgatgtcacc
     8041 acgaattaat tttatcatta cttgttgttt taaaatgtat tgttctctcg aatagcttcc
     8101 aagcctaact tttctctatt ttttctttgg taaatctttc cagatcaaag ttcaatcgag
     8161 ttacaaagcc ggtggttctt tccttgcaat cttttctttt acaatgcttg cagtatttct
     8221 taaaacattc atccgtgtgc acgttaaaaa caatatcttc ccggaaattg gagaccaata
     8281 acttctttag tttttcccct tcttcgtgag cctccgataa atataaattc caaggtaaaa
     8341 ccagatcgca atttatatga tatgcgttcc cgtatttgat gagtttaaaa ttgtgtatat
     8401 ctatccattc atcgggtttg ttatcttcaa ttatttt
//
LOCUS       DAOSTD010000075         8436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_7977_length_8436_cov_14.549576, whole genome shotgun sequence.
ACCESSION   DAOSTD010000075 DAOSTD010000000
VERSION     DAOSTD010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7977_length_8436_cov_14.549576"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PLI37_05880"
     CDS             <1..903
                     /locus_tag="PLI37_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOQ58571.1"
                     /translation="DTLLEMARKDKNIVVVTSDARGSVTLGDFASELPGQFIEVGIAE
                     QNAVGIGAGLASCGKNVFVCGPACFYVARSLEQVKVDVAYSHYPVRILGVSGGMSYGA
                     LGSTHHSLHDIAVLRTFPGMHIVLPADIYETRRLVMALMDYPHPVYIRVGRNAVPNVH
                     DDDKIDFTIGKAIRLAKGKDLTIIGTGETVYHCLEASRKLSEHGIQAGVLDMHSLKPF
                     DAEAVLQAAEESGRIITVEEHSMFGGLGAAVAETLVQHHPVPVKILGVPDENAVAGTP
                     LELFRHYSLDAEGIVKTALNWLRA"
     gene            900..2384
                     /gene="glpK"
                     /locus_tag="PLI37_05885"
     CDS             900..2384
                     /gene="glpK"
                     /locus_tag="PLI37_05885"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006282172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOQ58572.1"
                     /translation="MNEKFTLAIDQSTSATKFLLFDDQANLRHRLSLEHRQYYPRAGW
                     VEHDPVEILENVYKGVEALLNNSGVAPGDIVSVAITNQRESVLVWDAATSKPVSPVVV
                     WQCQRGAAICAELKNKGYEKMLRSRTGLIIDPYFSASGVQWILEEIPGLKERARAGEL
                     RMGTMDSWLIWNLTGGRVHATDQTNASRTLLYNIHSLSWDREILSLFGIPESMLPRVL
                     ACDQVFGNTDFRGLLPRAVPIAGVLGDSHGALVGQSCFTPGMGKATYGTGSSVMVNIG
                     CSPLKAPEGLVTSVAFAALGKTYYAFEGNIHCTGATIKWMEDELGLLTRSAESESLAL
                     SVDDNAGVYLVPAFAGLGAPWWKPDARAIICGLTRGSSKAHIVRAGLEAIAYQVKDLV
                     DLMLSQAGTTLEELRVDGGPVRNRFLMQFQADMLNAPINVSPIEEASALGAVYMNFLA
                     QGRVRSLEQLSQLKIRQEQLKPRMNAAQREQLYAGWQQAVKMIL"
     gene            2396..>3660
                     /locus_tag="PLI37_05890"
     CDS             2396..>3660
                     /locus_tag="PLI37_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HOQ58573.1"
                     /translation="MSYKKQLCFGVIIGTRNYFNSALAKDVRKSLLESLTKAGHTYVI
                     LDESQTATGSIETREDGIKCAKLFREHRDCIDGILISLPNFGFEIGIINALDLAELKV
                     PILVQACDDDNDKVGLDSRRDAYCGKISVCNNLYQYNIPFSLTSLHTCAIDSKEFAAD
                     LDYFAGICRVVRGLHKARIGAIGTRPAGFQTVRASEKILQASGITVVPVDLSEILAAA
                     NKYDNNSAELKQRVEEIKAYARVPEKFAEKLKVQAKFGLAVEAWIKENEVDATAIQCW
                     DSLEKNYGCAACVSMSMMSEKLIPSACETDIAGAISMYMLTLASGQPAALADWNNNFA
                     REHDKCVCTHCGNFPKSFVKNPLELGTLGVLGSVLGSEATFGAVKGKVSAGDFSFFRI
                     STDDNFGLIKAYLGEGEITDEPYGMDGCIA"
     assembly_gap    3661..3760
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3761..4547
                     /locus_tag="PLI37_05895"
     CDS             <3761..4547
                     /locus_tag="PLI37_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-ribose ABC transporter substrate-binding
                     protein"
                     /protein_id="HOQ58574.1"
                     /translation="AIELGYDAVLFDSQNNPSKETEHFDNILSSGYKAILFNPTDANG
                     SVVNVKAAKKAGIPVFCMDREINDSEAAVSQILSDNFSGCVALGQYFVKKFNGKGNYV
                     EILGLVGDNNTWNRSKGFHSVVDNFPQLVMLAQQTADFDQNKAMEVMESMMQAHSNID
                     AVFCGNDAMAMGAYQAILAAGKAGRILVFGFDGSQDAINAIKEGKMEATGMQFPKTMA
                     RTAAEFADRYLKGDRNFQKKVPVAVELVTPENVHKYGAFEKLE"
     gene            4551..5174
                     /locus_tag="PLI37_05900"
     CDS             4551..5174
                     /locus_tag="PLI37_05900"
                     /inference="COORDINATES: protein motif:HMM:NF021565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2291 family protein"
                     /protein_id="HOQ58575.1"
                     /translation="MRKSLKIILALVAFILALYFSFYTESLSERQLKEQFKEFNPAQV
                     VDYCWEHKLEDLVRHAIPCEEFFEGLQGDAESYAERQGKVLGIGSNYFFVMQARAEIS
                     SVDEHNLYLILNRDIKATIPLVNIFSNLARDASAWFNPDDFQNTMDFNALSSYLNKKI
                     LQEVLAPLSERFSPGVRIEFVAAVEVNINDLPLEELVLIPYKIEILS"
     gene            5184..6713
                     /gene="gguA"
                     /locus_tag="PLI37_05905"
     CDS             5184..6713
                     /gene="gguA"
                     /locus_tag="PLI37_05905"
                     /inference="COORDINATES: protein motif:HMM:NF040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOQ58576.1"
                     /translation="MYKSDPEILLEARHISKQFPGVLALDDVGFKVYAGKVNAIVGEN
                     GAGKSTLMNILSGVYSDYQGEVLLCGRPVSFSGTADAQRQGIAMIHQELNLVPYLSIA
                     QNIFLGREPLNALGLIDFPKMKRDTVDLLERLHFDESPEKLVIDLRPGQQQIVEIAKA
                     LSLQAKVLIMDEPTSSLSEKEAGILFRLIRELTENGVGVVYITHKMNEISLLADYVTV
                     LRDGCFIEACPMEQTSVDELVRKMVGRERKDFFIKRQHRLGECLLAVKDLCLRDPLRE
                     GKYRLKDISFEVYAGQVLGIYGLMGAGRTELMEALFGLYGKQVEGRVFVKGRQVKFNN
                     TNAAVAEGLALIPEDRKTGGLVLDMSIEQNTTLASLKNFLKHGLLSFKKERATAEEMR
                     SKLEIKSYSGLQLASQLSGGNQQKVVLSKWLLTKPGIVLLDEPTRGIDIHSKNEIYKL
                     MDDLAGQGMALVVVSSELPEIMAVSDKIICLCEGRLSGRFERDEFSEEALLKAALPDK
                     A"
     assembly_gap    6728..6827
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6875..7858
                     /gene="rbsC"
                     /locus_tag="PLI37_05910"
     CDS             6875..7858
                     /gene="rbsC"
                     /locus_tag="PLI37_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003235578.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HOQ58577.1"
                     /translation="MKKNFKVIIDKPVIKPLVALLLMVGVMSVLSGNFATTDNFFNVL
                     RQVSVNLCISVGMTMVILTGGIDLSVGSILALSGAVAAGLSKNGFSFPAADLYVGFTF
                     WGCILAALLIGAALGAFNGWMITRFKVPPFVATLAMLTIARGLTMLYTKGFPITGLGE
                     SFSFLGTGWFLGVPMPVWVALLIVAFFAFFMLKTRTGRYIYAIGGNKKASLLSGIKVN
                     KIILWVYTLAGILSAVAGLMVTARLDSAQPNAGSGYELDSIAAVVIGGTSLSGGKGSI
                     AGTVVGALIIGVLNNGLVLLNVSPFWQQVIKGFVILGAVILDKVSPGKSEN"
     gene            complement(7903..>8436)
                     /locus_tag="PLI37_05915"
     CDS             complement(7903..>8436)
                     /locus_tag="PLI37_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOQ58578.1"
                     /translation="PALSTAIIFREAKHSYRSFSQKAQYLWEEQEKKSWFNLGQRSFE
                     CTKLMMSIKIYTLLRQYGTALFEEYIDYTYDLAQSFAGMLKQESDLELALQPETNVVC
                     FRYKPGKLSSASLSKLNSLIRKKLIEDGEFYIVQTQLKEDIWLRVTLMNPFTTTVELR
                     ALLEKIKQLGRQLVSEQ"
BASE COUNT         2066 a         1846 c         2183 g         2141 t
ORIGIN      
        1 gatacgctgt tggaaatggc gaggaaggat aaaaatattg ttgttgttac cagcgatgca
       61 aggggttccg tcactttagg agactttgcc tcggaattac ccggacagtt tatcgaagtg
      121 ggtatagccg aacaaaacgc ggtaggcatc ggtgcgggcc tggcatcttg cggcaagaat
      181 gtttttgtct gcgggcctgc ttgtttttat gtggcacgta gtctggaaca agtcaaggtc
      241 gatgtggctt attcccatta tccggtgcgg atattgggtg tcagtggggg aatgagctac
      301 ggagccctgg gctcaacaca ccacagtctg cacgatattg ccgtgttgag gacttttccc
      361 ggtatgcata tagtgctgcc ggccgatatt tatgaaaccc gccgcctggt gatggctctg
      421 atggattatc cgcatcccgt gtacattcgt gtgggccgta atgccgtacc caacgttcac
      481 gacgacgata aaatagactt taccatcggc aaagccatcc gcttggccaa gggtaaagac
      541 ctgacgatca ttggcaccgg agaaacggta tatcactgcc tggaagcttc gcgcaagttg
      601 agcgagcacg gcatacaggc aggagtgctc gatatgcaca gccttaagcc tttcgatgcc
      661 gaagcagtgc tgcaggcagc cgaagaaagc ggccggatta tcacggtgga ggaacacagc
      721 atgtttggag gccttggtgc tgcggtggca gaaaccctgg tacagcacca tccggttccc
      781 gtaaaaatac tgggtgtgcc cgacgaaaat gcagtggccg gcacccctct ggaactcttc
      841 cggcattaca gcctcgacgc cgaaggcata gttaaaaccg ccctaaactg gctccgggca
      901 tgaacgaaaa attcaccttg gccatagatc agagtacttc ggcaaccaag tttttgttgt
      961 tcgacgacca agctaatctg aggcaccgcc tctcgctgga gcaccgtcaa tattatccca
     1021 gagccggttg ggtggaacac gatcccgtag aaattctgga aaatgtatac aagggcgtcg
     1081 aagccttatt aaacaacagc ggagtagctc ccggcgacat tgtttcggtg gccatcacca
     1141 accaacgcga aagtgtgctg gtatgggatg ccgccacttc caaacctgtc agcccggtag
     1201 tggtttggca atgccagcgg ggagcggcca tttgtgccga gctcaaaaac aagggctacg
     1261 agaagatgtt gcgttccaga accggtttaa ttattgatcc ctatttttcg gccagcggtg
     1321 tacaatggat actggaggaa attccgggtt tgaaggagcg ggcaagggcc ggagagctgc
     1381 gtatgggcac tatggattcc tggcttatct ggaatctgac cggaggccgg gtacatgcca
     1441 cagaccagac taatgcctcc cgtaccttgt tgtataatat tcacagcctg agttgggaca
     1501 gggaaattct ctctctcttt ggtattccgg aatccatgct gccccgggta ttggcttgcg
     1561 atcaggtgtt tggtaataca gactttcgtg gtctgttacc ccgggccgta cctatagccg
     1621 gagtactggg cgattctcac ggagccctgg tcggacaaag ctgctttacc cccggaatgg
     1681 gtaaagctac ttacggaaca ggttcctcgg tgatggtcaa cataggttgt tctcctctga
     1741 aggctcccga aggtttggta acttcggtgg cttttgccgc cctgggaaag acatattacg
     1801 ctttcgaagg caatattcat tgcaccggag caaccattaa atggatggag gacgaactcg
     1861 gcttgttgac ccgttctgcc gaaagcgagt ctctggcttt gagcgttgac gacaacgccg
     1921 gagtttacct cgttccggca tttgccggtt tgggtgcgcc ctggtggaaa cccgatgcca
     1981 gagctattat atgcggtttg actcgtggga gcagcaaagc gcacatagtg cgcgccggct
     2041 tggaggccat tgcttatcag gtaaaagatc tggtagatct gatgctcagt caggccggaa
     2101 caacactcga agagctccgg gttgacggag gtccggtacg aaaccgtttc ctgatgcagt
     2161 ttcaggccga catgctaaat gcccccatca acgtaagccc cattgaagaa gcttctgcac
     2221 tgggcgccgt gtatatgaat tttctggccc agggccgggt ccggagcctg gagcaactga
     2281 gtcaactcaa aatccgtcag gaacaactca agcctcggat gaatgctgca caaagagaac
     2341 aattatacgc cggctggcaa caagccgtaa aaatgatttt ataaacctta caactatgtc
     2401 atacaaaaag caactttgtt tcggggtcat aataggcacc cgtaattatt tcaactcggc
     2461 cctggccaaa gacgtgagaa aatctttgtt ggaaagcctt accaaagccg gtcacaccta
     2521 cgttatcctc gatgaaagtc aaacagccac cggcagtatc gaaacccggg aagacggcat
     2581 caaatgcgcc aaacttttca gggaacaccg cgactgcatc gatggaatat tgatttccct
     2641 gcccaatttc ggtttcgaaa taggtattat taacgccttg gatctggctg aactcaaggt
     2701 tcccattttg gtgcaggcct gcgacgacga caacgacaag gtaggcttgg acagcagacg
     2761 ggatgcctat tgcggaaaaa tatcggtttg caacaacctt tatcaataca acattccttt
     2821 cagcctgacc tccctgcata cctgtgccat agacagcaaa gagtttgccg ccgatttgga
     2881 ttattttgcc gggatttgtc gcgtggttcg tggtttacac aaggcccgca tcggtgctat
     2941 cggtactcgt ccggccggtt tccaaaccgt cagggccagc gaaaaaatct tgcaggctag
     3001 cggcatcacg gtggttccgg tggatttatc cgagatactg gcggctgcaa acaagtacga
     3061 caacaactcg gctgagctta aacaacgtgt cgaagaaatc aaggcttatg cccgggttcc
     3121 tgagaaattt gccgaaaaac tgaaagttca ggctaaattc ggattggccg ttgaggcctg
     3181 gatcaaagaa aacgaagtgg acgctacggc tattcagtgc tgggattcgc tcgaaaaaaa
     3241 ctatggttgc gcagcttgcg tcagcatgag tatgatgagc gaaaaactca tacccagcgc
     3301 ctgcgagacg gatattgcgg gggccatttc catgtatatg ctcactttgg cttcgggtca
     3361 accggcagct ttggccgact ggaacaataa ttttgcccgt gaacacgata aatgtgtttg
     3421 cacgcattgc ggtaatttcc ccaaaagctt tgtcaagaac ccgttagaac tgggaacatt
     3481 gggtgtattg ggtagtgtgt tgggcagtga agccactttt ggcgccgtca aaggcaaagt
     3541 ttctgcgggt gatttttcct tttttaggat ttcgaccgac gacaatttcg gcctgatcaa
     3601 ggcttacctg ggtgaaggcg aaattaccga cgagccctat ggcatggatg gttgcattgc
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agccattgaa ttggggtacg
     3781 atgctgtatt gtttgattct caaaacaatc cttcgaaaga aacggaacat tttgacaata
     3841 ttttgtcctc gggttataag gctattttat ttaatcctac cgacgccaac ggttcggtgg
     3901 tcaacgtaaa agccgccaaa aaggccggca tcccggtgtt ttgtatggac agggaaatca
     3961 acgattcaga agctgccgtt tctcagattc tttccgataa cttttcgggt tgtgtggctc
     4021 tgggacagta ttttgtaaag aaattcaacg ggaaaggcaa ttacgtagag atactaggtc
     4081 ttgtaggcga taacaatacc tggaaccgtt ctaaaggttt ccacagtgta gtggataatt
     4141 ttcctcaatt ggtcatgctg gctcaacaaa cagccgattt tgatcaaaac aaagccatgg
     4201 aggtaatgga gtcgatgatg caggcgcatt ccaatattga tgcggtcttt tgcggcaacg
     4261 acgctatggc tatgggagcc tatcaggcca tcctggccgc aggcaaggcc ggccgtattc
     4321 tggtctttgg ttttgacgga agtcaggatg ccattaatgc catcaaagaa ggtaaaatgg
     4381 aggctaccgg catgcaattt cccaaaacca tggctcgtac ggctgcggaa tttgccgacc
     4441 gctatcttaa gggtgaccgt aatttccaga aaaaagtgcc tgtggccgtg gaactggtta
     4501 cgccggaaaa tgtgcacaag tacggagcat ttgagaaact ggaataagcg atgcgaaaaa
     4561 gtttaaaaat catcctggcc ctggtggctt ttatccttgc tctgtatttt tctttttata
     4621 ccgaatcgct gagcgagagg caattgaaag aacaattcaa ggaattcaat cccgctcagg
     4681 tggttgatta ttgttgggaa cacaaactgg aggatttggt tcggcacgct attccttgcg
     4741 aagagttttt cgaaggtttg caaggcgacg ccgaatctta tgccgaaagg cagggtaaag
     4801 tgttgggcat aggctccaat tatttctttg taatgcaggc ccgggccgaa atttcctctg
     4861 tcgatgaaca taatctatac ctgattctga accgggatat aaaagcgacc attccattgg
     4921 taaatatatt cagtaacctg gctcgcgatg cctccgcctg gttcaatccc gacgattttc
     4981 aaaacacgat ggattttaat gctctgtcgt cgtacctgaa caaaaaaatt cttcaggagg
     5041 tactggctcc tctgagtgaa cggttcagcc ccggagtcag aatagagttc gttgctgctg
     5101 tcgaggtaaa tatcaacgat ttgcccttgg aagaactggt gctgattccc tataaaatcg
     5161 aaatactaag ttaaggcggt tccatgtata agtccgatcc cgaaattctg ctcgaagcca
     5221 ggcacatcag caaacagttt cccggcgtgt tggccttgga cgatgtcggt tttaaggttt
     5281 atgcaggtaa agtaaatgcc attgtcggtg aaaacggggc aggcaaatca accctgatga
     5341 atattttgtc gggggtgtat tccgactatc agggagaagt cttgctttgc ggccggccgg
     5401 taagcttttc gggaacggcc gatgcccaga ggcagggaat agccatgata catcaggaat
     5461 tgaatctggt tccttatttg agtattgccc aaaatatttt tttaggcagg gaaccattga
     5521 atgccttggg attgatagat ttcccaaaaa tgaaaaggga tacggtcgat ttgctcgaaa
     5581 gattgcattt cgacgaaagt cccgaaaagt tggtcataga cctcagaccc ggtcagcaac
     5641 aaatagtgga aattgcgaaa gctctgtctc ttcaggccaa agttttgata atggacgagc
     5701 ctacttcttc cttgtccgaa aaagaggccg gaatattgtt tcgcttgata agggaactca
     5761 ccgaaaatgg agtgggagtg gtgtatatca ctcataaaat gaacgagata agcctattgg
     5821 ccgattatgt aaccgtgttg agagacggtt gttttatcga agcttgcccg atggaacaaa
     5881 ccagcgtgga tgaattggtg cgcaagatgg tcggaaggga acgcaaggat ttttttataa
     5941 aaagacaaca tcgcttggga gaatgcctgc ttgccgtcaa agatctttgc cttagggacc
     6001 cattgcgcga aggcaaatac cgcctgaaag acataagttt tgaagtgtat gccggccagg
     6061 tattgggtat ttacggcttg atgggtgccg gacggaccga attgatggaa gccttgtttg
     6121 gtttgtacgg caagcaagtg gagggccggg tgtttgtcaa agggaggcaa gttaaattta
     6181 ataacactaa tgccgcggtg gcagagggct tggcgcttat tcccgaagac agaaaaaccg
     6241 gaggactggt tctcgacatg tcgatcgagc aaaatacaac tctggcttct cttaaaaatt
     6301 ttttgaaaca tggcctgctt tcgtttaaaa aagaacgggc aacagcagaa gaaatgagaa
     6361 gcaaactgga aattaaatcg tattccggtt tacaattggc ttctcaactc agcggaggca
     6421 atcagcaaaa agtggttctg tccaagtggt tgctgactaa gcccggcatt gtattattgg
     6481 acgaacctac ccggggcatc gacattcact ccaaaaatga aatttataaa ttgatggacg
     6541 atttggccgg ccaaggcatg gctttggtgg tggtatcttc tgaattaccg gagattatgg
     6601 ctgtgtccga taaaataatt tgcctctgcg agggtaggtt aagcggacgt tttgaacggg
     6661 acgagttttc tgaagaagcc ttattgaaag cagccttacc cgataaggct taaatacccg
     6721 ataaggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntac ccgataaggc
     6841 ttaaataccc gataaggcct aaatagcagt gattatgaaa aagaacttta aagtcattat
     6901 agacaaaccg gtaattaaac ctctggtcgc tctactgctg atggtgggag taatgtccgt
     6961 gctttcggga aattttgcca ccaccgacaa ttttttcaat gttttgcgcc aggtttcggt
     7021 taacctttgt atatcggtgg gaatgaccat ggttattctg accggaggca tcgatctttc
     7081 ggtgggttcc attctggcgc tgagcggtgc tgtggcggcc ggactgagca aaaacggttt
     7141 cagctttccc gcagcagacc tttacgtggg ctttaccttt tggggctgta tactggccgc
     7201 tttacttatc ggtgctgcgc tgggagcctt caacggctgg atgatcacac gcttcaaggt
     7261 gccgcctttt gtggctactt tggccatgct gaccatagcc aggggattga ccatgttgta
     7321 caccaagggt ttccccatta cgggtctggg cgagagtttt tcctttttgg gtaccggctg
     7381 gtttttggga gtgcctatgc cggtgtgggt agccctgttg atcgtagcct tctttgcctt
     7441 ttttatgctc aaaacccgca caggacgtta tatttacgcc atcggaggca ataaaaaagc
     7501 cagcttgctg tcgggtatca aggtgaataa aattatcctc tgggtgtaca ccctggcggg
     7561 aatattgtct gccgtagccg ggctgatggt tacagccaga ttggattctg cccagcccaa
     7621 cgcgggcagc ggttatgaac tcgattccat agccgccgtt gttattggag gcacttccct
     7681 gagcgggggc aagggatcca ttgccggtac ggtggtgggc gcgttgatta tcggggtgct
     7741 caacaatggt ttggttttgc tgaacgtttc tcctttctgg cagcaagtca taaagggttt
     7801 tgtcattctt ggagccgtta tcctcgacaa ggtcagtccc ggaaaatcgg aaaactaaca
     7861 tactattttt ggaaaggccg gttttatccg gtggtacttc ctctattgct ctgacacgag
     7921 ttgcctgcca agctgtttga ttttctccag taatgcccgc agttccacgg ttgtggtaaa
     7981 gggattcatc agggttactc tgagccagat atcttctttg agctgggttt gtacgatata
     8041 gaattctccg tcttcgatca acttctttcg aatcaagctg tttagtttgc ttaagcttgc
     8101 gctgctcagc ttgcccggct tataacgaaa gcaaacaaca ttggtttcgg gttgcagtgc
     8161 caattccaga tcgctttcct gtttgagcat accggcaaaa gactgggcca ggtcgtaggt
     8221 ataatcgatg tattcttcga acaaggccgt accgtattgt ctgagcagag tgtatatctt
     8281 aatactcatc atcagcttgg tgcattcaaa gcttctttgc ccgagattga accaggattt
     8341 tttttcttgt tcttcccaca ggtactgtgc tttttggctg aaactgcggt aagaatgttt
     8401 tgcctcacgg aaaataatgg ccgtagaaag ggcagg
//
LOCUS       DAOSTD010000076         8181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_8285_length_8181_cov_14.880384, whole genome shotgun sequence.
ACCESSION   DAOSTD010000076 DAOSTD010000000
VERSION     DAOSTD010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8285_length_8181_cov_14.880384"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PLI37_05920"
     CDS             <1..780
                     /locus_tag="PLI37_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58579.1"
                     /translation="GVRTPGFEKGAQSCDVVLKSHYNRKYKYPKNFIPWQFGLSNRMI
                     EAVHPLPFKQREQAFLVNFRVKHQLRDFLNNRLRAIVEKSLIWDNSISDLEKEKLTGK
                     DLLFWKQTGGRHYPSYYDKLSKTVACACYGGVFAIPWGNYNKYTAKIARLLNDLIPLF
                     PWDRVRQWDSWRLWESWVAGCCVVHVDFDKYGCQLPVMPQNKIHYLGIDLDNLEDFEA
                     SICDKALLEQIAENGKKFVLQHYSPEAVAGRLLQTLNLKSG"
     gene            829..3291
                     /locus_tag="PLI37_05925"
     CDS             829..3291
                     /locus_tag="PLI37_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58580.1"
                     /translation="MKTKPFHLALPHLIAVAVFLIITLIYFAPAAFEGKDLVQGDITN
                     AKATGVDIREHFKATGENVFWSNTMFGGMPANYTAMPPTTNVFRVFGEMFTLGLPKSH
                     LGQFFLALLGFYIFLLALDCKPWLSMIGAIAYAFASYNLIIIGAGHMNKGLVMATMAP
                     ILGGIILCYRKKFLWGGLITLIFTGLNILWGHQQITYYLILTIVILALVYLYYAFKEK
                     QLKNFVLSSAVLLVLAGLAVLPALGFLIPSADYAKESMRGGAVLQTDTEGKKESSGLE
                     IDYAFAWSYGKAETMTLLIPNFYGGSSHYALGTDSECYKKLRSTGQARQFCRAAPTYW
                     GAQPFTSGPVYAGAIICFLFVLGLFIVKGPEKWWLLLATVLAILMSWGHNFMAFNEFL
                     FNHLPLYNKFRVPSMSLVIANLAMAALGILAVKELLDHRKDAQFAQTYFKPLYLSFAI
                     VGGLSLIIAMFGSSMFDFSGNSDANFPAWLVDALKADRQQLLRSDAWRSFLYILLAFT
                     LIWFFIKKPFKEIYVVLGLGLLITLDLWTVDKRFLNHSDFVPKQKAKEILPSEADLQI
                     LQDKDPNYRVLNLTTSTFNDARTSYFHKSIGGYSGAKLRRYQDIIDYHFSKGINTNVL
                     NMLNTRYIILPSQEQQGKTMVQRNSQALGNAWFVEKITWVDGPNAEIVALNDFNPAKE
                     VFIDKAWQHLLPNSSTLEQTTDSSAYIRLSDYRSPGNLFYESNNPRAQLAVFSEVYYK
                     TWQVFIDGGRVPLLRVNYILRGLEIPAGQHVIEFKCVDEVYERSAALSKWSSVIVGLL
                     ILGFTGFAVFKAVKKSTKRKFE"
     gene            3312..4088
                     /locus_tag="PLI37_05930"
     CDS             3312..4088
                     /locus_tag="PLI37_05930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ58581.1"
                     /translation="MPPKVSVITITYNAGEFLEKTLLSVLNQTSSDLEYILVDGASTD
                     NTTDLIRQYEDLVNKGQFAIGPGQFRWISEADRGLYDAMNKGLKMASGEFVWFINAGD
                     KIYDNNSLELILNKLNDYPQSDVLYGQSLIIDQNDNALGERHKTAPAKLNKTSLLMGL
                     VVCHQSILVRRSLAPQYDLSYRISADYDWVCKVLSLSGQNTYVDAYISRFMVSGISAR
                     QRKKSLKERFHIMRKHFGLGATLGAHFILALRYPFTRKYH"
     gene            4090..4869
                     /locus_tag="PLI37_05935"
     CDS             4090..4869
                     /locus_tag="PLI37_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOQ58582.1"
                     /translation="MKKIIGFFTARIPRQWLIRFSYIFSFLIRPFYIGNKVECPVCES
                     RFRKFLPYGYGESADNRLCPKCLSLERHRLLWLYLKQKTLFFEAKLKVLHFAPEQPFL
                     RRFRALKNLDYTTADLDSPIADLKLDLCDIALPDNSYDVLLCNHVLEHVPDLDKALAE
                     IKRVLKPGGWAILLVPLNMNVNTFGDPSITDPKERQRLYGQYDHVRQFGKDYPDILRK
                     AGFLVTEDRFYYELSAQQRERQHLARVGEEIIYRCDKSLSL"
     gene            complement(4886..5821)
                     /locus_tag="PLI37_05940"
     CDS             complement(4886..5821)
                     /locus_tag="PLI37_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ58583.1"
                     /translation="MKRILVTGGAGFLGSHLCDQLLARGNEVICLDNYFTGSKQNVIH
                     LLDNPYFELLRHDITNPVYVEIDEIYNLACPASPVHYQYDAIKTIQTSVMGAINMLGL
                     AKRTEAKVLQASTSEVYGDPIVHPQPEEYWGNVNPIGIRSCYDEGKRCAETLFFDYYR
                     QNKVKIKVVRIFNTYGPRMHPNDGRVVSNFIVQALKGQDITIYGDGLQTRSFCYCDDL
                     IAGLITMMDTGDSVTGPINLGNPTEFTIKQLAELVIELTGTKSDIVYKPAVSDDPMQR
                     KPVIEKAKQVLNWEPRIQLREGLMRTIEYFDSILK"
     gene            complement(5828..6817)
                     /locus_tag="PLI37_05945"
     CDS             complement(5828..6817)
                     /locus_tag="PLI37_05945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008777702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ58584.1"
                     /translation="MDKELQLSIVVPVYNRPGEIGELLESLCAQTDPDFEVLIVEDGS
                     TLDCEEVCRQYSEKLQLNYLFKPNSGRSDSRNYGMERARGNYFLIFDSDCLLPPDYIQ
                     KVRTHLREHYLDCYGGPDKAAGDFTNRQKAINYAMTSFLTTGGIRGGGKKKASFTPRS
                     FNMGISKEVFRQVGGFKNIVGEDIDLSIRIKKAGFSTGLLSDAFVYHKRRVDLKSFYR
                     QVKTFGKGRVLISRMHPGSMKLLHLLPLIFVLVHVLLLLLSLFFLNPWFLLPLPVYML
                     ILFLDSLFKNKHAGIAALSVLAAYIQLFGYGLGLGNELLTKKAYTSTQEELYK"
     gene            6917..>8181
                     /locus_tag="PLI37_05950"
     CDS             6917..>8181
                     /locus_tag="PLI37_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOQ58585.1"
                     /translation="MGIIIRQSVKGTAVSYVGIAIGFFTTFFVLTNFLTAEEIGLTRV
                     LVDAATVFAGLAQLGTTSSAIRYYPYFKEGEKDHGFFFWTLVVPFIGFLIYGILFWLL
                     KEPISGFFAENSPLFVDYYNFIFPVGFFLLYMSVFEVNSNLLLRIVVPKFIREVVVRL
                     LSLAAYLLYAFDLISLDGFIIAFSSVYGIATILNIVYIFSLKKISLKPDFKFLKKSLV
                     KDYLLYTLFLIAAALGGVIAPSINSFFISAKMGLNFAGIFAIAGYMASMIEMPYRSLG
                     TISGPHISQAIKDQDFTRINALCKSISLHQLLGGFFIFLAIWINIDLIFELLPNGEIY
                     AAGKWVVFILGLSRLLNSVFNVSEIVLRYSKFYYVSLILTVILTTSAIILNNSLIPVW
                     GMNGAAIASLLSYLIFYFLLLSLVRWKIKT"
BASE COUNT         2164 a         1854 c         1807 g         2356 t
ORIGIN      
        1 ggagtacgca cccccggttt cgaaaaagga gctcaaagtt gcgatgttgt tcttaaaagt
       61 cattacaacc gcaaatacaa gtatcccaag aactttattc cctggcaatt tggtttgtcc
      121 aacagaatga tagaggcggt acaccctctg ccgttcaagc aacgggaaca ggcttttttg
      181 gtgaacttca gggtaaaaca tcaattgcgg gactttttga acaatcgatt aagagctatt
      241 gtcgaaaaga gcctgatctg ggataacagc atcagcgatc ttgaaaaaga aaaattgacg
      301 ggcaaggact tattattttg gaaacaaacc ggaggcagac actacccctc gtactacgat
      361 aaattatcca aaacggttgc ctgcgcctgt tatggaggag tatttgccat cccctggggt
      421 aattacaaca aatacacggc caaaattgcc cggttactca acgaccttat ccccctgttt
      481 ccctgggaca gagtaagaca atgggattcc tggagacttt gggaaagctg ggtagccggc
      541 tgctgcgtgg tgcatgtgga cttcgataag tatggttgtc aactgcctgt gatgcctcaa
      601 aacaaaattc attacctggg cattgatctg gacaatcttg aagatttcga agcctctatc
      661 tgcgacaagg cgcttctgga acaaatcgcc gaaaacggaa aaaaatttgt attacaacat
      721 tattccccgg aggctgtcgc cggaagatta ttacaaacat tgaatttaaa atcgggctga
      781 ttctctacat tttgcctatt tttgtttttg tatacgaaat attgccctat gaaaaccaag
      841 cctttccatc ttgcactgcc tcatctgatt gcagtcgcag tgtttttaat cattactctg
      901 atctattttg ctcctgctgc tttcgaaggc aaagacctgg ttcagggaga tataaccaat
      961 gccaaagcca caggcgttga cattagagaa cattttaagg ccaccggcga aaacgttttt
     1021 tggtctaaca cgatgttcgg cggcatgcct gccaattata cggctatgcc tccaaccacc
     1081 aacgtttttc gcgtttttgg agaaatgttc acactgggct taccaaaatc acatctcgga
     1141 cagttttttt tagccctgct gggattttat atttttctgc tggctcttga ctgcaagcct
     1201 tggttaagca tgatcggagc cattgcctat gcctttgcct cctacaattt aattattatt
     1261 ggagcaggac acatgaacaa aggcctggtt atggcaacca tggcacccat cctgggaggc
     1321 attatacttt gctaccggaa gaaattttta tggggagggt tgattacctt gatatttacc
     1381 ggactcaata tcttatgggg acatcagcaa atcacttatt atctcatctt gaccatagtc
     1441 atcttggcgt tggtttacct gtattatgcc tttaaagaaa aacaattaaa aaattttgta
     1501 ctttcttctg cggtattgct tgtgctggcg ggtttagccg ttcttccggc tctgggattt
     1561 cttattccat cggccgatta tgccaaagaa agcatgcgag gaggagccgt cttgcaaacc
     1621 gacaccgaag gcaaaaaaga aagctccggg ctggaaatcg attatgcttt tgcctggagc
     1681 tacgggaagg ccgaaaccat gaccttgctt attcccaatt tttacggagg tagttcgcat
     1741 tatgccttag gaaccgattc cgagtgctat aaaaaattga gaagtacagg tcaggccagg
     1801 caattttgtc gggcggctcc cacttattgg ggtgctcaac cttttacatc gggacctgtg
     1861 tatgccggcg ccattatctg tttccttttt gttctgggat tattcatagt aaaaggcccc
     1921 gaaaaatggt ggctattgct ggccaccgtt ttggctatat tgatgtcctg ggggcataat
     1981 tttatggctt tcaatgaatt cctttttaat catttgcctt tgtacaataa attcagggtg
     2041 ccctccatgt cgctggttat agccaatctg gccatggctg ccctgggaat tcttgccgta
     2101 aaagagctct tagatcatcg caaagacgca caatttgcac agacttattt taaaccctta
     2161 tacttatcct ttgcaattgt tgggggatta tccctgatta ttgccatgtt tggaagcagt
     2221 atgttcgatt tttcgggcaa ttccgatgcc aacttccctg cctggctggt agacgcgctc
     2281 aaggccgaca ggcaacagtt actgcgttcc gatgcctggc gttcttttct gtatattctg
     2341 ttggccttta ctttgatctg gttctttatc aaaaaacctt tcaaagagat ctacgtcgtc
     2401 ctgggcttag gtttgcttat aacactggat ttgtggactg tagataaacg cttcttaaac
     2461 cacagcgatt ttgtaccaaa gcaaaaagcc aaagaaatac tgcctagcga agccgactta
     2521 caaattttgc aagataagga tcccaattac cgggtgctca atttaacgac cagcactttt
     2581 aacgatgccc gtacttcgta ttttcacaaa tccattggag gttacagcgg agccaaactc
     2641 aggagatacc aggatataat cgattatcat ttctcgaagg gcatcaacac gaatgtattg
     2701 aatatgctca atacccgtta tattattctg ccttcgcagg aacagcaggg caaaaccatg
     2761 gtccagcgaa actcgcaggc attggggaat gcctggtttg tggagaaaat cacctgggtt
     2821 gacggcccca acgccgaaat tgtcgccttg aacgatttca accccgccaa agaggtgttt
     2881 atcgacaaag cctggcaaca cttactgccc aacagctcta ccctggaaca gacgaccgat
     2941 tcaagtgcct atattcgtct atctgattac cggagtcccg gcaacctgtt ctacgaaagc
     3001 aataatcccc gggcacagtt ggcggtcttt tccgaagtgt actacaaaac ctggcaagtt
     3061 tttattgacg gcggacgggt acccttgttg cgggtgaact atattctgcg cggactggaa
     3121 attccggccg gccagcatgt cattgaattt aaatgcgtgg atgaggttta cgaacgaagc
     3181 gctgctttat ccaaatggtc gtccgtaata gtgggactgc tgattctggg ctttacaggc
     3241 tttgccgtgt tcaaagccgt caaaaaatcc actaaaagaa agtttgagta aaaagcaact
     3301 ctctttgcaa aatgccacct aaagtcagcg tcataaccat cacctacaac gccggggagt
     3361 ttttggaaaa aaccctcctg agcgtactga accaaaccag ctccgatttg gaatatatct
     3421 tggtagacgg agcttctaca gacaacacaa ccgatctgat ccggcagtac gaagacttgg
     3481 tgaacaaggg gcaatttgct atcgggcccg gtcaattccg ctggatcagc gaagcagacc
     3541 ggggcttata cgacgccatg aacaaaggcc tgaaaatggc cagcggagag tttgtatggt
     3601 ttattaatgc cggcgataaa atatacgaca acaacagcct cgagctcata ctgaacaaac
     3661 tgaatgatta tccccaaagc gacgtgctgt acggacaatc gctgattatc gatcaaaacg
     3721 ataatgccct aggcgaaaga cataaaaccg ctcccgctaa attaaataaa acaagcctgc
     3781 tcatgggcct ggtcgtctgc catcagtcca ttttggtacg tagaagcctg gctccccagt
     3841 acgacctgag ttaccgcatc tcggccgact acgattgggt ttgtaaagtt ctttccctgt
     3901 ccgggcaaaa cacctatgtt gacgcttata tttcccgctt tatggtttcg ggcatttcgg
     3961 cccggcagcg taaaaaatct ttgaaggaac gttttcacat catgcgtaag cattttggac
     4021 ttggtgctac actgggagcc cattttatac tggcactgcg ttatcccttt acccgaaaat
     4081 accattgaca tgaagaaaat aataggcttt tttacagccc gtatacccag gcaatggttg
     4141 atacgcttca gctatatctt cagttttctg atcaggccat tctatattgg taataaagta
     4201 gaatgccccg tttgtgaaag tcgattcaga aaatttttac catatggtta cggggagagt
     4261 gccgacaacc gactttgtcc caaatgttta tccttagaga gacaccgctt gttgtggctc
     4321 tacctgaaac aaaaaacctt gttttttgaa gcaaagctca aagttttgca ctttgcaccg
     4381 gaacaaccct ttctcagacg cttcagagct ttgaaaaacc tggactacac aacggccgac
     4441 ctggactcgc ctattgcaga tctcaagctg gatctttgtg atatcgcctt gcccgacaac
     4501 agttacgatg tcttgctatg caaccacgta ttggagcatg tccccgatct ggacaaggct
     4561 ctcgccgaaa tcaaaagggt actgaagccg ggaggctggg ccattttgct ggttccactt
     4621 aatatgaacg ttaatacttt tggcgatccg tccataacag accccaaaga gcgtcaacgt
     4681 ttgtacggcc agtacgacca cgtacgccag tttggaaaag attaccccga catactaaga
     4741 aaagccggct ttctggtgac agaagaccgg ttttactacg aattaagcgc tcagcaaaga
     4801 gaaaggcagc acctggccag agtgggcgag gaaattattt atcgttgcga taaatcactt
     4861 tcactttaat cgttttccgg agaatttatt ttaagatgga atcgaaatat tcgatggtgc
     4921 gcatcaaacc ttccctgagc tgtatgcgcg gttcccagtt gaggacctgc ttagcctttt
     4981 ctataacagg tttgcgctgc ataggatcgt ccgaaactgc cggcttgtaa acaatatccg
     5041 atttggtgcc ggtcaattct attaccagtt ctgccagttg ctttatggta aattctgtag
     5101 ggttgcctaa attgatgggg ccggttacgg aatctccggt atccatcatg gttatgaggc
     5161 ctgcaatcag atcgtcgcaa taacaaaaac tacgggtctg cagcccatct ccgtatatgg
     5221 tgatgtcctg tcctttcagt gcctgcacta tgaaattgga aaccaccctg ccgtcgttgg
     5281 gatgcattct gggtccatag gtattgaaaa tcctgactac cttgattttg actttatttt
     5341 gcctgtaata gtcaaaaaac aaagtttctg cgcaacgttt gccctcgtcg taacaggaac
     5401 gaataccaat gggattgacg ttgccccaat actcttcggg ctgcggatgc acaatgggat
     5461 ctccgtaaac ttcgctggta gaagcctgta agactttggc ttctgttcgt tttgccagac
     5521 ccagcatatt gatggctccc atcacggaag tctgtatcgt tttgatggca tcgtattgat
     5581 aatgtaccgg cgaagcggga caggccagat tgtagatttc gtcgatttct acatacacgg
     5641 ggttggtgat gtcgtgccgc aacaattcaa aatagggatt gtccagcaaa tggatcacat
     5701 tctgcttaga tcctgtaaaa tagttatcaa gacagatgac ttcgttacca cgggccaata
     5761 attgatcaca aaggtgagaa cccaaaaatc cggctccgcc ggtaaccaat attcttttca
     5821 taatgtatta tttatacaat tcttcttgtg tcgatgtata agctttcttt gttaaaagtt
     5881 cattgccgag ccccagcccg taaccaaaca gctgaatata ggcggccagc accgataaag
     5941 ctgcgatgcc tgcgtgtttg ttcttgaaca aggaatcaag aaaaaggatg agcatgtaga
     6001 ccggcagggg caacaaaaac cagggattca aaaaaaacag gctcagcagg agcaagagca
     6061 catgaaccag aacaaatatc agaggcagca ggtgtagcaa tttcattgaa cccggatgca
     6121 tacgggaaat aagcacccgg cctttgccaa aagtttttac ctgcctgtaa aagctcttta
     6181 aatctactct gcgtttatga taaacaaaag catccgaaag taaaccggta ctgaaaccgg
     6241 cctttttgat tcgaatactc agatcgatgt cttcgcccac tatgttttta aaacctccca
     6301 cctgccggaa aacttctttt gagattccca tattaaagga ccgtggcgta aaggaagcct
     6361 tctttttgcc tccaccgcgt ataccaccgg tagtcagaaa agaagtcatg gcgtaattga
     6421 tggccttttg tctatttgta aaatcgccgg ctgctttgtc gggtcctcca tagcaatcca
     6481 ggtaatgctc ccgcaagtga gttctgactt tttgtatgta gtcgggaggt aagaggcaat
     6541 cggagtcgaa gattaaaaaa taattgcctc gggctctctc catgccgtag ttccggctgt
     6601 cgcttcgacc cgaattgggc ttaaaaaggt agtttagttg cagtttttcg ctgtattgcc
     6661 tgcaaacctc ttcacagtcg agcgttgatc cgtcttccac tattaggact tcgaaatcgg
     6721 ggtccgtctg ggcgcacaaa ctttcgagga gttctccgat ttcgcccggt ctgttgtaaa
     6781 ccggaacaac gatagataac tgcaattctt tatccatgaa tacttaggta gataatccgc
     6841 aaaaatagga aaaaaaacaa tctaagcgtt tattcgccta aaatacgtac attcgcaacg
     6901 gaaacagagt gcatttatgg gcataatcat ccggcaaagt gtcaaaggaa cagcagtgag
     6961 ttatgtgggt attgccatcg gctttttcac cacttttttt gtccttacca atttccttac
     7021 ggccgaagag atagggctga cacgtgtgtt ggtcgacgcc gccaccgtat ttgccggtct
     7081 tgctcaatta ggcaccacct catcggccat acgctattat ccctatttca aagaaggaga
     7141 aaaagatcat ggattctttt tttggaccct ggttgtccct tttataggct ttttaatcta
     7201 cggtatacta ttctggttgc ttaaagagcc catttcgggg ttctttgccg agaattcccc
     7261 gttgtttgtc gattactaca actttatctt tccggtaggg ttcttcttac tttacatgtc
     7321 tgtttttgag gtgaattcca atttgcttct cagaattgtg gtgcctaaat ttatcaggga
     7381 agtagtcgtc aggctattgt cgctggctgc ctatctcctt tatgccttcg atctgatcag
     7441 tctggatggt tttattattg ctttcagctc ggtatacggc atagcaacca ttttaaacat
     7501 cgtttatata ttcagcctta aaaaaatttc tctcaaacct gattttaagt ttttgaagaa
     7561 atcgctggtc aaggattatc tcctttacac cttattttta atcgcagcgg ccttaggagg
     7621 cgttatcgct cccagcatca attctttttt catcagcgcc aagatgggct tgaattttgc
     7681 cggcatcttt gctattgcag gctatatggc ttccatgatt gaaatgcctt acagatcgtt
     7741 gggtaccatt tcggggccac acatctctca ggccataaaa gatcaagatt tcacaagaat
     7801 aaacgcccta tgtaagagta tttctttaca tcagttgctg gggggcttct ttatcttttt
     7861 ggctatctgg attaatatcg acttgatttt cgagctttta cccaatgggg aaatttatgc
     7921 ggcgggaaaa tgggtggtat ttatccttgg actgagtcga ttgttgaatt ctgtttttaa
     7981 tgtgagtgaa atcgtgttgc gctactcaaa attttactac gtatcgctca tcttaactgt
     8041 tattttgacg accagcgcaa ttattctcaa caattcactg ataccggtct ggggcatgaa
     8101 cggagctgca atagcttctt tgttgtcgta tctgattttt tattttttgt tgcttagttt
     8161 ggtgcgctgg aaaataaaaa c
//
LOCUS       DAOSTD010000077         8107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_8392_length_8107_cov_16.343766, whole genome shotgun sequence.
ACCESSION   DAOSTD010000077 DAOSTD010000000
VERSION     DAOSTD010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8392_length_8107_cov_16.343766"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PLI37_05955"
     CDS             <1..597
                     /locus_tag="PLI37_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HOQ58586.1"
                     /translation="FAGADTLATSYALSKAIQKMGQIDLIIGGRQAIDGDTAQVGPQV
                     AEKLNLYQVTYAEEILELDPKNKQIRIKRRLPRGIEVVLSPMPVLITVNGSAKACRPR
                     NAKRVLKYKYALTRFETEENPKFKALCDQRSYLNIPVWDVDFIEADKDLCGLSGSPTK
                     VKKIESVVFQAKENMTLESTDAEIETLIKTLLENHTIG"
     gene            625..1497
                     /locus_tag="PLI37_05960"
     CDS             625..1497
                     /locus_tag="PLI37_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOQ58587.1"
                     /translation="MNRNVYVRSVKFFFTGLLAAFLLASCGSVFLTGRKQLMLVSDQE
                     VLALSLQQYRSFMDSAEVVVNSADAEMINRIGRSIAESVENFMTFNGRAHELENIDWE
                     FSLVKDNSVNAFAMPGGKVVVFTGMLPVAESEAHLAVVIAHEIAHVIAKHSTERISQQ
                     IALQFGGEIAGGLLASSSETKRSIATAVFGLGAQLGVMMPYARKQEYEADELGLLFMA
                     MGGYHPQEATAFWTKMSAAGGTAIPEFLSTHPTDAKRIAKIQQKMPDALYYYKGPGII
                     NRTTAPIVKDTKVL"
     gene            complement(1505..2998)
                     /locus_tag="PLI37_05965"
     CDS             complement(1505..2998)
                     /locus_tag="PLI37_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOQ58588.1"
                     /translation="MTGIKSLAKDTAIYGLSSIVGKFLNWLLVPFYSYTLKETGDYGM
                     VTELYGWTALALVILTYGMETGFFRFINNDKYKSTQVYSTSLSMLGLTSLLFVAVSLI
                     FLPQISAGMGYEKHPEFIGMLIVVVAADAFCSIPFAYLRYKNKALRFASIKLLMILTN
                     IFFNLFFIWLCPKIYRWNPALIDWFYKPDYGVGYVFVSNLFSTGITLLALLPYFVKVK
                     FDFNPVLLRKMLGYSLPLLLLGIAGIMNQTFDKIIFTHLFEDQQYARAQLGIYGACYK
                     IAVVMMMFTQAFRYAYEPYIFAKHRDDDNRRAYAEAMKYYIIFAFLIFLGIMFYLDIF
                     KYIISSDYHEGLKVVPIVLMCYLFQGVFFNLSLWYKLTDRTQWGAYISMFGAFITVLG
                     NILFVPRYGYMAAAWVSFVCFFLMMVISWWLGQKYYPICYDLKSAGKYLLLTAGLYAL
                     GMLLPVEPLWLKLLFRTALLLIFLLVLTKKDLPLNQLLPGKLLHKKD"
     gene            complement(3005..4027)
                     /gene="ruvB"
                     /locus_tag="PLI37_05970"
     CDS             complement(3005..4027)
                     /gene="ruvB"
                     /locus_tag="PLI37_05970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOQ58589.1"
                     /translation="MDDQFDIRSPRLNESEQDFEKVLRPLSFADFRGQEKVVENLKIF
                     VSAASMRNESMDHVLLHGPPGLGKTTLSNIIANELGVGFKSTSGPVLDKPGDLAGVLT
                     ALEPNDVLFIDEIHRLSPVVEEYLYSAMEDYRIDIMIDKGPSARSIQLDINPFTMIGA
                     TTRSGLLTAPLRARFGINCHLEYYQVEVLSGIVSRSARILDVPCSTEAAFEIARRSRG
                     TPRVANALLRRIRDFAQVKGNGKIDLNITKYALLALNIDTHGLDEIDNRILTTIIDKF
                     QGGPVGLTTIATALGEDPGTLEEVYEPFLIQEGFLKRTPRGREVTELAWRHLGRNYQG
                     DFQRLF"
     assembly_gap    4112..4211
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4214..5449
                     /locus_tag="PLI37_05975"
     CDS             4214..5449
                     /locus_tag="PLI37_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOQ58590.1"
                     /translation="MVLNYIWVGFFVVAFVFALIQSLFFNDYAVFERIMNSSFDMAKF
                     AVMDICLPLVGIMSLWLGFMNIGEKAGAIRFLSRIVGPFFSKLFPGVPKDHPVNGELL
                     MNFSANMLGLDNAATPLGLKAMQSLQELNPHKESASNAQIMFLALNTSGLTIIPISII
                     AQRAILGAANPTDVFVPIIIATYVSTLTAILYVGFRQRLSLFNKTVAAWLGGISLLLA
                     ALVFWFTQMDRESMRTASSLLSNGTLLGIIILFMAGGLYKKINIWESFIDGAKNGFKT
                     SVKIIPYLVGMLVGIGVFRASGAMDFLTDGIAWFFNSLRIDTDFVDALPTALMKPLSG
                     SGARAMMVEAMKMNGADSFVGRLACIFQGSTDTTFYIVALYFGSVGIKRTRYAVSAGL
                     IADLAGIVASILLAYLFFH"
     gene            5461..5898
                     /gene="ybeY"
                     /locus_tag="PLI37_05980"
     CDS             5461..5898
                     /gene="ybeY"
                     /locus_tag="PLI37_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOQ58591.1"
                     /translation="MIQFHGEDIALPELGKNLICAWVEEVAAGYGKKTGQINYIFCSE
                     KKMAEINMRYLNHDYFTDIIGFDYSSGQSISGDLFISPLTIASNAELLKQPFQRELHR
                     VLIHGILHLCGFEDKTTSQQTIMRVEENKALELLYIIEKEQSS"
     gene            5913..7787
                     /gene="mnmG"
                     /locus_tag="PLI37_05985"
     CDS             5913..7787
                     /gene="mnmG"
                     /locus_tag="PLI37_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOQ58592.1"
                     /translation="MNFSYDIIVVGAGHAGCEAATAAANMGSKTLLITMDMNKIAQMS
                     CNPAMGGIAKGQIIREIDALGGYTGIVSDKTAIQFRMLNRSKGPAMWSPRSQNDRQQF
                     IQAWRQVVENTDNLFLWQDTVNELLIENGTVKGVKTELNVIFRAKSVILTNGTFLNGL
                     IHIGQNQIKGGRISERASVGLTEQLRELGFSYGRMKTGTPVRVDARTVDFSKLSEQKG
                     ENDFHKFSYLDFTPRKLKQRSCWICFTNERVHELLREDLELSPMYNGQIQSIGPRYCP
                     SIETKIVNFSDKDQHQMFLEPEGEDTQEYYLNGFSSSLPLETQLKALREVSGFEQVHI
                     YRPGYAIEYDYFDPVQLKATLETKLIPGLFFAGQINGTTGYEEAAGQGLVAGVNAHIF
                     CHGGQEFVLNRDEAYIGVLIDDLINKGVDEPYRMFTSRAEYRILLRQDDADARLTEKS
                     YALGLATAERLNLWKEKQKEIESLLLFARQYSIKAKYINTALESLDTAPLKQGVRLSD
                     LILRPQLSFDILQEHIPALKRRIEQIPNRRDEIVEAAEIQIKYQGYIEREQLQAEKTQ
                     RLDNIRIKDKFDYLSIHSLSFEARQKLDKIQPETLGQASRIPGISPSDISILLVLLGR
                     "
     gene            7821..>8107
                     /locus_tag="PLI37_05990"
     CDS             7821..>8107
                     /locus_tag="PLI37_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOQ58593.1"
                     /translation="MQTPSSHINQILSGLPELPGIYQYLGEEGRIIYVGKAKNLKKRV
                     LSYFNKNHNDSPKTAILVKRIRDIRYTVVENEQDALLLENNLIKEHQPRYNV"
BASE COUNT         2349 a         1849 c         1835 g         1974 t
ORIGIN      
        1 tttgcaggag ccgacaccct ggccacctct tacgccctct cgaaagccat ccaaaaaatg
       61 gggcaaatcg atttaatcat tgggggaaga caagccatcg acggagatac cgcacaagtg
      121 ggcccccagg tagccgaaaa actcaaccta taccaggtaa cctacgctga agaaattttg
      181 gaactcgatc cgaagaacaa acaaatacga attaagcgtc gtctgcccag aggaatcgaa
      241 gtggtacttt cgcccatgcc cgtgctgatt acggtaaacg gctctgctaa agcctgcaga
      301 ccgcgtaatg ccaaaagagt tcttaaatac aaatacgctc ttacaagatt cgaaaccgaa
      361 gaaaatccta aatttaaagc cctttgcgac caaagaagct atttaaacat tccggtttgg
      421 gacgtggatt ttattgaggc cgataaagat ctttgcggtt tatccggctc tccaaccaag
      481 gttaaaaaaa ttgaaagcgt tgttttccaa gccaaagaaa acatgacact tgaatcgaca
      541 gatgccgaaa tcgagacctt gatcaagact ttgcttgaaa accacactat aggataagta
      601 ttactacttt cattattgaa tcctatgaac agaaatgttt acgtccggag cgtaaaattt
      661 ttcttcaccg gcttattggc tgcttttctt ttagcatcct gcggcagcgt ttttcttacc
      721 ggaagaaaac aactgatgtt ggtttccgac caggaagtgc tcgcattaag cttgcaacaa
      781 tatcgcagtt ttatggacag cgctgaagtc gtcgttaatt cggccgacgc agaaatgatc
      841 aatcgtatcg gccgctcaat agcggaatct gtagaaaatt ttatgacatt caacggcaga
      901 gcccacgaac tggaaaacat agactgggaa ttcagcctgg ttaaagacaa cagcgtcaac
      961 gcttttgcca tgcccggagg caaagtggtt gtttttaccg gtatgcttcc cgtggccgaa
     1021 agcgaagccc acttagctgt tgtcattgca cacgaaatcg cccatgtcat cgccaaacat
     1081 tccacggaaa gaatcagtca acaaatcgcc ctgcaattcg gcggggaaat tgccggcggg
     1141 cttttggcca gctcgtctga aaccaagcga agcattgcca ccgcggtatt cggactggga
     1201 gcacagctgg gagtaatgat gccttacgcc cgcaaacagg aatacgaggc cgacgaactg
     1261 ggtctgcttt tcatggctat gggcggctac catcctcagg aggctactgc tttttggacc
     1321 aaaatgtctg cggccggagg gactgcaatt ccggagtttt tgagcactca cccaaccgat
     1381 gccaagcgca tagccaaaat tcagcaaaag atgcctgatg ctctgtacta ttacaaaggg
     1441 ccgggcatca ttaacagaac cacggcccct atagtcaaag acactaaggt tttgtaaatc
     1501 ctacttagtc ttttttatgc agaagtttac ccggaagcaa ttggttcagg ggtaggtctt
     1561 ttttggtcaa taccagaagg aaaatcagca gaagcgccgt tctgaaaagt aatttcagcc
     1621 acaaaggctc aacaggcaga agcatcccca gagcatacag gccggcggta agcagtaaat
     1681 attttccggc cgatttcaga tcatagcaga tggggtaata cttttgcccc aaccaccagg
     1741 aaatcaccat cataagaaag aaacagacga aagaaaccca agctgccgcc atatagccgt
     1801 agcggggtac aaaaagaatg tttcccaaaa cggtaatgaa agcgccaaac atagaaatat
     1861 aagcacccca ttgggttcgg tcggtcagct tataccagag agaaagattg aaaaagactc
     1921 cctgaaacag atagcacatc aaaacaatgg ggactacctt caagccctcg tgataatcgg
     1981 agctgataat gtatttgaaa atgtcgaggt aaaacataat ccccaggaaa ataagaaaag
     2041 caaaaatgat gtaatacttc attgcttcgg catacgcgcg tcgattatcg tcgtctcgat
     2101 gtttggcaaa aatatagggt tcgtaggcat aacgaaaagc ctgagtaaac atcatcataa
     2161 ccacggctat tttataacaa gctccgtaaa tacccaattg agccctggcg tattgctggt
     2221 cttcgaacag gtgagtaaaa ataatcttat cgaaggtctg gttcatgatg ccggcaattc
     2281 caagcagaag caggggcagt gaatagccaa gcattttcct cagtaaaacc ggattgaagt
     2341 cgaattttac tttgacaaaa tagggcagta aggccagtag tgttatgccc gtagagaata
     2401 aattggatac aaaaacgtaa cccaccccgt aatcgggctt gtaaaaccaa tcgatcaaag
     2461 ctggattcca ccggtaaatt tttggacaga gccaaataaa aaacaagttg aaaaagatat
     2521 tggtgagtat catcaataac ttgatcgagg caaaacgcaa ggccttattt ttgtaacgta
     2581 aataagcaaa gggaatggag caaaaggcgt cggcggccac aactacgatt agcatcccta
     2641 taaattcagg gtgcttttca tagcccatac cggccgaaat ctgaggtaaa aaaatcaaag
     2701 aaaccgccac aaaaagcagg gaggtcagtc ccaacatgct cagactggtg gaataaacct
     2761 gggtcgactt atacttgtcg ttgtttatga aacggaaaaa accggtttcc atgccgtagg
     2821 tcagaatcac caaggccaaa gccgtccacc cgtatagctc ggtgaccatg ccgtaatcgc
     2881 cggtttcttt gagtgtgtag gagtaaaagg gaaccagcag ccaattcaag aattttccga
     2941 cgatactgct taagccatat attgcagtgt ccttggccaa cgatttgatt cctgtcattc
     3001 cgtcttaaaa caaacgttga aaatctccct ggtaattgcg gcctaaatgc ctccaggcca
     3061 attcggtaac ctctcttcct ctgggtgtac gttttaggaa accttcttga atcaaaaaag
     3121 gttcatacac ctcttccaac gtgccgggat cctcgcctag agccgttgca atggtggtca
     3181 ggcccaccgg cccaccctgg aatttatcga taatggtggt caggattctg ttgtcgattt
     3241 catccaggcc gtgagtatca atattcaggg ccaataaggc atatttggta atattcaagt
     3301 cgatctttcc gttgcctttt acttgggcaa aatcgcgaat acgtcgcagc agggcgttag
     3361 ctacgcgggg cgtgcccctg cttcgccggg cgatttcgaa ggcagcttcg gtactgcagg
     3421 gcacgtcgag tattcgggcc gaccttgata ctataccgga caaaacctca acctggtagt
     3481 actccaaatg acaattaatg ccgaaacgag ccctcagggg agcagtaagc agaccgctgc
     3541 gagtggtggc cccaatcatg gtaaatggat tgatgtccaa ctgtattgac ctggcgctag
     3601 ggcctttgtc tatcataatg tcgatgcggt aatcctccat ggccgaatac aggtattctt
     3661 cgaccacagg gctcaagcga tggatttcgt cgatgaacag aacatcgttg ggttccaggg
     3721 cagtcaacac tccggccaaa tcgcccggct tatccaacac agggcccgaa gtggatttga
     3781 aacccacacc caattcgttg gcaattatat tggacaaagt cgttttgccc aagcccggag
     3841 ggccatgcaa caaaacgtga tccatggatt cattgcgcat cgaagcggca gaaacaaaaa
     3901 ttttgaggtt ctctaccact ttttcttgtc ctcgaaaatc ggcaaaactg agcggacgaa
     3961 ggactttttc gaaatcctgt tcgctttcgt tcagcctggg gctgcgtatg tcaaactgat
     4021 cgtccatgtt gtgaagtgag gctcaaagat acacgcattc ggggaattta gaaagagcgg
     4081 gggaagaatt tttgggctac gcccaagaat annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn ngtatggtgc tcaattatat ttgggtaggt tttttcgttg ttgcttttgt
     4261 ttttgctctg attcaaagct tgttctttaa cgactacgca gtttttgaac ggatcatgaa
     4321 cagcagtttt gatatggcaa aattcgctgt tatggatatc tgcctccctt tggtgggcat
     4381 tatgtctttg tggctgggct ttatgaacat aggcgaaaaa gcgggggcta ttcgtttttt
     4441 atcccgtata gtggggccct tcttttcaaa acttttcccc ggcgtcccca aggatcatcc
     4501 ggtcaacggt gaattattga tgaatttttc ggctaatatg ttagggttag acaatgccgc
     4561 cactccactg ggcttaaaag cgatgcaaag cctgcaggag ttgaatcctc acaaggaatc
     4621 tgcctccaac gctcaaatta tgtttttggc cttgaacacc tcggggctca ccattattcc
     4681 tatcagcata attgcccaac gagccatact cggcgcagca aacccgacgg atgtgtttgt
     4741 tcccattatt attgccacct atgtgtctac tctgacggcc atactttacg tgggttttcg
     4801 ccagcgcctg tcgctgttca acaaaacggt ggcagcctgg ctgggaggta tcagcctgtt
     4861 gcttgccgct ctggtgtttt ggttcacaca aatggacaga gaaagcatga gaactgcttc
     4921 cagcctgctg agtaacggca ctttgctggg tattatcatt ttgttcatgg ccggtggctt
     4981 atacaaaaaa ataaacatct gggaaagctt tatcgacggt gccaaaaacg gttttaaaac
     5041 ctctgtaaaa atcatccctt atctagtagg tatgctcgta ggcatcggag ttttcagagc
     5101 atcgggtgcc atggattttc ttaccgacgg tattgcctgg tttttcaaca gcctgcgcat
     5161 cgacaccgat tttgtggatg ctctacccac ggctttgatg aaaccattga gcggaagtgg
     5221 cgccagggcc atgatggtag aagcaatgaa aatgaatgga gccgattcct ttgtaggccg
     5281 gctggcctgc atatttcagg gatcgaccga caccaccttt tacatcgttg cactgtactt
     5341 cggatcggtg ggtataaaac gtacacgcta tgctgtatcg gccggattga tcgctgatct
     5401 tgccggtatt gttgcttcca tcttgctggc ttatctgttt tttcattgat aaacttgctt
     5461 atgattcaat ttcacgggga agacattgcc ctgccggaac tgggaaaaaa cctgatctgc
     5521 gcctgggtag aagaagtggc agccggctac ggcaaaaaaa ccggtcaaat caactacatc
     5581 ttttgctccg aaaaaaaaat ggctgaaatt aatatgcgat atcttaacca cgattatttt
     5641 accgatatca tcggttttga ttatagttca ggacaaagca tttcggggga tttatttatt
     5701 tctcctttaa ctattgcatc caacgccgag ctattaaaac agccttttca acgagaactg
     5761 cacagagttt taattcacgg catcttacat ctctgcggtt ttgaagacaa aacaacaagc
     5821 caacaaacaa tcatgcgcgt ggaagaaaac aaagcgctgg aattgcttta tatcatagag
     5881 aaggaacaaa gttcctgaat tgaatttcca ttatgaactt ttcgtacgac ataatagtag
     5941 ttggagcggg tcacgccggc tgtgaagccg ccactgctgc cgccaacatg ggctccaaaa
     6001 ccctgttgat tactatggat atgaacaaaa ttgcccagat gagctgcaac cctgccatgg
     6061 gaggaatagc caaaggccag attatcagag aaatagatgc gcttggaggc tatacaggca
     6121 tcgttagcga caaaacagcc attcagtttc ggatgctcaa tagatccaaa ggccctgcca
     6181 tgtggagtcc acgctcacaa aacgaccggc agcaatttat tcaggcatgg cgacaagttg
     6241 tagaaaatac cgacaatcta ttcctttggc aggatacggt taatgaatta ttgatagaaa
     6301 atggtaccgt aaaaggggta aaaacggaac tcaacgtaat tttcagagcc aaaagtgtca
     6361 tacttaccaa cggtactttt ctaaacggat tgatacacat cggacaaaat caaattaaag
     6421 gaggccggat ttcagaaaga gcttccgttg gtttaactga acaactgaga gaactgggat
     6481 tcagctacgg cagaatgaaa accggcactc cggtgcgtgt ggacgcaaga acggtagatt
     6541 ttagcaagct aagcgaacaa aagggggaaa acgattttca taaattttct tacctcgact
     6601 tcacacctag gaaactaaaa caacgcagct gttggatctg ctttactaac gagcgtgtgc
     6661 acgaacttct gagagaagac ctggaactct cgcccatgta caacggacaa attcaaagca
     6721 tagggccccg ctattgcccc agtattgaaa ccaaaatcgt taatttcagc gataaggatc
     6781 aacaccagat gtttttggag ccggagggag aggataccca ggaatactac ctgaacggtt
     6841 tttcctcttc cctgcccttg gaaacccaac taaaagcgct tagggaagtt tccggctttg
     6901 aacaagtcca tatttaccgt ccgggctacg ccatagaata cgactacttt gatccggtcc
     6961 aactcaaagc cactctggag accaagttga tccccggttt attttttgcc ggccagatca
     7021 acggcaccac cggctacgag gaagcagccg gtcaaggctt ggtggctgga gtcaacgcac
     7081 atattttttg ccacggaggc caagaattcg tattaaacag agacgaagcc tatattggcg
     7141 ttctcattga cgatttaatc aacaagggag tagacgaacc ctaccgcatg tttacttcca
     7201 gagccgagta ccgcatattg ttaagacaag acgatgccga cgctcgtcta acggaaaaat
     7261 cgtatgcgct ggggctggct acagccgaac gcctgaatct ttggaaagaa aaacaaaaag
     7321 aaattgagag cctgctgctg tttgcccggc agtattccat taaggctaaa tatataaaca
     7381 ccgccttgga gagtctagat acagctcccc ttaagcaagg agtccgcctg agcgatctga
     7441 ttttgcgccc acaattgtct tttgatattt tgcaagaaca tattcccgca ctcaaaagaa
     7501 gaattgagca gatccccaat cgccgggacg aaatagtcga agcggccgaa atccaaatca
     7561 agtaccaggg ttatattgaa cgcgaacagt tacaggcaga aaaaacgcaa aggctcgaca
     7621 atattcgaat caaagataaa ttcgactatt taagcataca ttccctatcg ttcgaagcaa
     7681 gacaaaaatt agataaaata cagcccgaga ctttaggtca ggccagccgc attccgggaa
     7741 tctctcccag cgacatcagt attttgctgg ttttgctggg gagataggcc cgatttgttc
     7801 cacgtgaaac atttaagccg atgcaaacac cctcgtcgca tataaaccaa atattaagcg
     7861 ggctgcccga actccccggc atttatcagt acttgggaga agagggccgc atcatttatg
     7921 tgggcaaggc aaagaattta aaaaaaagag tcctctcgta ttttaataag aatcacaacg
     7981 attctccaaa aacggccatt ttagtcaaaa ggatcagaga tattcgctat actgtggtcg
     8041 aaaacgaaca agacgctctt ttgctcgaaa acaacctgat aaaagaacac caacccaggt
     8101 acaatgt
//
LOCUS       DAOSTD010000078         7665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_8975_length_7665_cov_15.146781, whole genome shotgun sequence.
ACCESSION   DAOSTD010000078 DAOSTD010000000
VERSION     DAOSTD010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7665
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8975_length_7665_cov_15.146781"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PLI37_05995"
     CDS             <1..1020
                     /locus_tag="PLI37_05995"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ58594.1"
                     /translation="VKRMEDALELPARNVWLSSLPGAEATNPAAVNYLHILDNSPSNE
                     PMSYEVVLAQHHNELIVDSLRIVLCQGDVYSFGGQSITEAGIYYDTIYAESAPDTLQI
                     LRLELLSRSYVELSDSICIGESYTGNGFNLPPHQEAGTFVYTDTLQNQWGCDSILHLS
                     LAVCAPPEAPGPIYGDSMVVVAGNYTYTIKPVPNAAFYIWTVLPPYWEKTVQGHSLSL
                     NIPYLGSGTISVRAVNCCGESAGSSLQINGQSGFDGQETTSSFSVFPNPSTSGFVLET
                     SGIEGETTICISSMTGQILHRQTKYLSAEDSRFEFSLDDYVQGMYIISIVNEQVSGVE
                     KLIRE"
     gene            1089..>1376
                     /locus_tag="PLI37_06000"
     CDS             1089..>1376
                     /locus_tag="PLI37_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOQ58595.1"
                     /translation="MKAVLFAAGLGTRLYPHTLDKPKALVSIQGRPMLYRVMDKLYEA
                     GIREYVVNVHAFAGQVEKALELYARSKPGISFDISDERDLLLETGGGLKKMA"
     assembly_gap    1377..1476
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1477..1888
                     /locus_tag="PLI37_06005"
     CDS             <1477..1888
                     /locus_tag="PLI37_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58596.1"
                     /translation="LQSQVVPPQSQVQPQTQDQLQTGGQYPLAVLAVRNDFKNRCFLL
                     NQHNLLCGWENKRSGEQIISRQQEALHPVGFMGIHCISPSIFPLMEEEGVFSIIDVYL
                     RLARQHIILGLDQSACKWMDIGSPEQLLLAEKQL"
     gene            1946..2626
                     /locus_tag="PLI37_06010"
     CDS             1946..2626
                     /locus_tag="PLI37_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOQ58597.1"
                     /translation="MKRQLSVAYMLCGLLFTVCLIVANIVEQKTIALGKIEATAGLLI
                     FPVSYIVNDLISEVWGYRKARLIIWSGFLMNFLAVAIFQLSIHVPGSVNFHHQEAFSQ
                     VLGNTLRLTLASFIAFLCGSFLNAFVMSKMKLRHGGRRFSLRAIVSTLAGEGVDSLVF
                     FSIAFTGILPPSDLLMLILTQTGMKTAYEILVLPLTSKIVVYLKKVEKVEVFDQDISY
                     NPFKISDL"
     gene            complement(2671..3135)
                     /gene="mscL"
                     /locus_tag="PLI37_06015"
     CDS             complement(2671..3135)
                     /gene="mscL"
                     /locus_tag="PLI37_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOQ58598.1"
                     /translation="MSFIGEFKKFALKGNVVDMAVGIIIGAAFSKIVSSAVADVIMPP
                     IGLLIGGVNFTDLVWKIKPETLDELGNVIAPAVTLNYGNFLQVTLDFLIVAFAVFLLV
                     KFINELAKKTEELTKKKQAEEAEAPAEPVQPVMSREESLLTEIRDLLAEKIK"
     gene            3382..4242
                     /gene="rfbA"
                     /locus_tag="PLI37_06020"
     CDS             3382..4242
                     /gene="rfbA"
                     /locus_tag="PLI37_06020"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008026599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOQ58599.1"
                     /translation="MKGIILAGGSATRLFPLSIAISKQIMPIYDKPMIYYPLSTLMLA
                     GIREVLVISTPRDLPMFRELLGSGESLGMSFQYKVQEQPNGLAQAFVLGADFLNGGPA
                     CLILGDNLFYGQGFSSMLKRAVALERGACIFGYYVKDPRPYGVVEFDENGKVLSLEEK
                     PSQPKSNYAVPGLYFYDERVTRFASELKPSARGEYEITDLNKVYLKEGSLQVELFGRG
                     FAWLDTGNSDSLLEASNFVATIQNRQGFYVSCIEEIAWRNRWIDNEQLRKLGEKLNKT
                     AYGQYILSLV"
     gene            4239..>5308
                     /gene="rfbB"
                     /locus_tag="PLI37_06025"
     CDS             4239..>5308
                     /gene="rfbB"
                     /locus_tag="PLI37_06025"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOQ58600.1"
                     /translation="MKTYLVTGGAGFIGANYVKYLLANYPDIRIIILDALTYAGNYGT
                     IAGDVDGEGCIFVKGNICDQKTVRNLFETYPIEVVVNFAAESHVDRSIENPQLFLETN
                     ILGTQNLLDEAKRAWTTGKDSQGYPAWKENVRFHQVSTDEVYGSLGDDGYFTEQTPLD
                     PRSPYSASKTSADLFVNAYVETFKMPATITRCSNNYGPYHFPEKLIPLIIKNILEGKP
                     LPVYGKGINVRDWLYVEDHCKAIEMVIRKGRPGEVYNVGGHNEKQNIEIVKMTIDSIR
                     EIMEKEPEYRSVLKTDIERINYDLITYVADRLGHDMRYAIDPTKIGNELGWYPETKFE
                     EGIQKTIRWYLKNQDWVNDVTSG"
     assembly_gap    5309..5408
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5409..5720
                     /gene="rfbC"
                     /locus_tag="PLI37_06030"
     CDS             <5409..5720
                     /gene="rfbC"
                     /locus_tag="PLI37_06030"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOQ58601.1"
                     /translation="AVDLRQNSPHFGRHVAVELSDENKRQFFIPRGFAHGFLVLSPEA
                     VFTYKVDNVYAPQAEGSLSYADPELGIPWPIAGDELILSPKDAQAPLLKDAYTFPGLL
                     P"
     gene            complement(5693..>7665)
                     /locus_tag="PLI37_06035"
     CDS             complement(5693..>7665)
                     /locus_tag="PLI37_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOQ58602.1"
                     /translation="KTDWLPVTKKEMELRGWEQADVVLFSGDAYVDHPSFGAAVAGRL
                     LEACGMKVALVPQPDWRGDHRDFTKMGKPRYFFAISAGCMDSMVNRYTANKRLRSDDA
                     YTADGLPGARPDYTTITYANILKKLYPEVPIVIGGIEASMRRLSHYDYWSDRLMPGIL
                     VDSKADLLVYGMGEKPLKEIAGLLLSGAPFEALRELRQVAYLRQAGDTAGKEISSCIQ
                     LHSHEECLNSKRKQAENFRVIEEESNKMEASFLIQRVGEMEIVVNPPHPPLSSEELDA
                     SFELPYTRLPHPKYKGKPIPAYEMIKFSVNLHRGCFGGCSFCAIAAHQGKFVVSRSKE
                     SVLKEVSLLSQRSDFKGYLSDLGGPSANMYRMQGQNQEICRKCKRNSCLFPKICSNLN
                     VDHRPLLDLYQAVDALPGIKKSVISSGIRYDLLLYSSQQALKSGECNQTAIKEAADKD
                     RAAQEYTRQLIEKHVSGRLKVAPEHTENSVLNSMRKPPFSQFIQFREIFEQIENRRLS
                     EQRKNQNFRTNEPHKNQNLLSTESPKGESKIKGRQELVPYFISSHPACSEEDMAELAA
                     KTKTLNFRLEQVQDFTPTPMTLATEMYYTGLNPYTLKPVYTAKTREEKLAQRQYFFWY
                     RPEDKASIVKALRRLKRVDLIEKLYGKRPGKV"
BASE COUNT         1897 a         1643 c         1829 g         2096 t
ORIGIN      
        1 gtaaaacgca tggaagatgc cctggaattg ccggcaagga acgtctggct gagttcgctc
       61 ccgggtgccg aagccaccaa cccggcggcc gtcaattacc tgcatattct cgacaacagc
      121 ccttcgaacg agcctatgtc ttacgaagtt gtacttgcac aacaccacaa tgagctgata
      181 gtcgacagtc tgagaattgt tctttgtcag ggcgatgttt acagctttgg agggcagtcg
      241 attaccgagg cgggtattta ttacgacact atttatgccg aatcggcgcc cgataccttg
      301 cagattctaa gactggagtt gttgtcacgc agttatgttg aactgtcgga cagtatctgt
      361 atcggtgagt cctacacagg gaacggattc aatctgccac cgcatcagga agcagggaca
      421 ttcgtttata cagatacttt gcagaatcaa tggggctgcg acagtatcct gcatttgagt
      481 ctggcggttt gtgctccgcc cgaagctccc ggcccgattt acggcgacag tatggtggta
      541 gtggccggca attataccta taccattaaa cccgttccta acgccgcttt ttatatctgg
      601 acggtattgc ctccctattg ggaaaaaaca gtgcagggtc atagcctgag tttgaatatt
      661 ccttatttgg gttccggcac tatttcggtt agggctgtaa attgttgcgg ggaatcggcc
      721 gggagcagtt tgcagatcaa tggacaatcc ggttttgacg ggcaggaaac gaccagcagt
      781 ttcagtgtgt tccccaatcc ctctacttcg ggctttgtac tggaaacttc cggcatagaa
      841 ggggaaacga ccatttgtat ctcgtccatg acgggacaaa ttctgcacag acaaaccaaa
      901 tacctgagtg ctgaagacag tcgttttgaa ttctcgctgg acgactatgt tcagggaatg
      961 tatataatca gcatagtcaa tgagcaagtt tcaggggtcg aaaaattgat tcgtgaataa
     1021 gcatttcgcc gggataggga aacttacttg tctaagtttc cctatctttg tcgttcaatg
     1081 catctgaaat gaaagcggtc ttgtttgctg cgggtttagg cacccgttta tatccccaca
     1141 ccctggataa gcccaaagcc ctggtcagta tacagggacg tcccatgctt taccgggtaa
     1201 tggacaaact gtacgaggcc ggtatcaggg aatatgtggt aaatgtacac gcctttgccg
     1261 ggcaggtgga aaaggccttg gagctttacg cccggagcaa gcccggcatt tctttcgata
     1321 tttcagacga aagggacttg ttgctcgaaa ccggaggagg cctgaaaaaa atggcgnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngctt caatctcagg ttgttccgcc
     1501 tcaatctcag gttcaacctc agactcaaga ccaacttcaa accggcgggc agtatcctct
     1561 ggcggtgctg gccgttcgga acgattttaa gaatcgatgt tttttgctta atcaacacaa
     1621 tttactctgt ggttgggaaa acaagcgaag cggggaacaa ataatttcca gacaacaaga
     1681 agccttacat cctgtggggt ttatgggcat acattgtatt tcaccttcca tttttccttt
     1741 aatggaggaa gaaggtgttt tttccataat cgatgtttat ttgcgcttgg cccgtcagca
     1801 tattatactg ggcctggatc agagtgcctg taaatggatg gacataggca gtccggaaca
     1861 attgttgctg gcagaaaagc aattgtagat ttatttctta attttgcagc tccgtgtgct
     1921 tgaaaaccac gttaaaaaca agattatgaa aagacaattg tctgtagcct acatgctctg
     1981 cggcttgctt tttacggttt gcctgatcgt ggccaacata gtggaacaaa aaaccatagc
     2041 tctgggaaaa attgaagcta ccgcaggctt gttgattttc cctgtttctt atatcgtaaa
     2101 cgatttaatt tccgaagttt ggggctatcg taaagcccgt ttgattatct ggagcggttt
     2161 tttaatgaat tttctggccg tagccatttt tcagctaagt attcatgtgc ccggctcggt
     2221 taattttcat catcaggaag ctttctcgca ggttttgggt aatacattgc gattgacttt
     2281 ggccagtttc atagcttttt tatgcggctc ttttttaaat gcctttgtga tgagtaaaat
     2341 gaagttgcgt cacgggggca ggcggttctc acttagggcc atagtatcta ccctggccgg
     2401 tgaaggtgtc gactccctgg tgtttttcag tatagctttt acaggcatat taccgccctc
     2461 ggacctgttg atgctgatac taacccaaac agggatgaag acggcctatg agattcttgt
     2521 actgccttta acttcaaaaa ttgttgttta tctaaaaaaa gtagaaaaag tcgaagtctt
     2581 tgaccaggat atctcttata atccatttaa aattagtgat ttatagagat tttgccttct
     2641 ttgaatttcg accttattca ggctgttctt ttacttgatt ttttcagcca ataaatcgcg
     2701 gatttcggtc aataaacttt cttctctgga cataaccggt tggacaggtt ctgcgggagc
     2761 ttccgcttct tcagcttgtt ttttcttggt gagttcttcc gtttttttgg ccagctcgtt
     2821 tataaattta accaaaagga aaacggcaaa agcaacaatc agaaaatcga gggtaacttg
     2881 cagaaaatta ccgtaattca acgttacggc aggtgcaatt acattgccca gttcgtccag
     2941 ggtttcgggt ttgattttcc agacgaggtc ggtaaaattg acaccgccta tcaacaaccc
     3001 aatagggggc atgatgacat cagccaccgc agaggataca atcttactaa aggcagctcc
     3061 gataatgata ccgacagcca tgtctaccac gttgcctttg agggcaaact ttttaaattc
     3121 tccgataaaa ctcataagga tttaaataaa gggtgaataa ttgaataaca aagaataaaa
     3181 tcaaaaagtt taaacaagga ttaaaatgcc ggagatggaa gtaaaatgta ctccttgcac
     3241 cggggcaaat gtaaacaata atttagaata aatatgcacg aaacattcct tcaaaaaagg
     3301 aaataaagca ggggcataag tttgcaatat ttaagtgtat gcttatcttt gtgttttctc
     3361 gcaaaaccta aaaaacccac aatgaaagga attatattag ccggcggaag cgccacccga
     3421 ttgttccctt tgtcaatcgc tatttccaaa caaatcatgc ctatttacga caagcccatg
     3481 atttattacc ccttgtctac acttatgttg gccggcatac gcgaagtgtt ggtgatttca
     3541 actcccagag atttacctat gttccgggaa ttactcggca gcggagaatc gctggggatg
     3601 tcgtttcaat ataaagtaca ggagcagccc aatggtctgg ctcaggcttt tgtgttggga
     3661 gcggatttct taaacggagg gccggcttgt ctgattttag gtgataacct gttttacggc
     3721 cagggctttt cctcgatgtt aaaacgtgct gttgctcttg aacggggagc ctgtatcttc
     3781 ggatattacg tgaaagatcc ccgtccttac ggtgtggttg agttcgatga aaacggcaag
     3841 gttttgtctc tcgaagaaaa accctcgcag cccaaatcga attacgccgt tccaggtcta
     3901 tatttttacg atgagcgggt cactcgtttt gcttccgagc ttaaaccctc ggccagagga
     3961 gaatatgaaa ttacggacct gaacaaagta tacctgaagg aaggcagttt gcaggtggaa
     4021 ttgtttggcc gcggcttcgc ctggctggat acaggtaaca gcgacagttt gctggaagcc
     4081 tccaatttcg tggctaccat ccaaaatcgc cagggttttt acgtgtcttg catcgaagaa
     4141 atagcatggc gtaaccgttg gatcgataat gaacaattgc gaaaactggg agaaaaatta
     4201 aataaaacag cctacgggca atacatttta tctttggtat gaaaacttat cttgtcaccg
     4261 gtggcgccgg ttttatcgga gccaattacg taaaatattt gttggctaat taccccgata
     4321 ttcggataat cattctggat gctcttactt atgccgggaa ttacggcacc atcgccgggg
     4381 atgtggacgg ggaaggctgt atttttgtca aaggcaacat ttgcgatcaa aaaacggtca
     4441 gaaatttgtt tgaaacttat cccatagagg tggttgtgaa ttttgctgcc gaaagtcacg
     4501 ttgatcgcag catcgaaaat cctcaattat ttttggaaac caatattttg ggtactcaaa
     4561 atttactgga tgaagccaag agagcctgga ccaccggtaa ggactcgcag ggttatccgg
     4621 catggaaaga gaatgttcgt ttccatcagg tttctaccga cgaagtgtac ggaagtctgg
     4681 gtgatgacgg ctattttacg gagcaaacac ccttggatcc ccgcagcccc tacagcgctt
     4741 cgaaaaccag tgccgatctg tttgtcaacg cctatgtgga gacgtttaag atgcctgcca
     4801 ccattacccg ttgttccaac aactacggcc cttatcattt ccccgaaaaa ttaattcctc
     4861 ttattatcaa aaatatactc gaaggcaaac ctctgcctgt ttacggaaaa gggattaatg
     4921 ttcgggactg gctttacgtg gaagaccatt gcaaagccat cgaaatggta atacgtaaag
     4981 gtcgtcccgg cgaggtctat aacgtgggag gacataacga aaaacaaaac atcgaaatcg
     5041 tcaaaatgac catagattcc attcgcgaaa tcatggaaaa agaaccggaa taccgttccg
     5101 tactaaagac cgacatcgaa cgcatcaatt acgatttgat tacctacgtg gccgaccgcc
     5161 tggggcacga tatgcgttat gccatagatc ccaccaaaat aggcaatgaa ttgggttggt
     5221 atcccgaaac caaattcgaa gaaggcatac aaaaaacgat acgctggtat ctgaaaaatc
     5281 aggactgggt aaacgatgtt acttcgggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnngc cgttgatttg aggcaaaatt ctccgcattt tggtcgacac gtagctgtgg
     5461 agctttcgga cgagaacaaa agacaatttt ttattccccg ggggtttgct catggctttt
     5521 tagtactaag ccccgaagcc gtatttacct acaaagtgga caatgtctat gctcctcagg
     5581 ccgagggcag tctttcttat gccgatcccg aattgggcat accctggccc attgcgggag
     5641 atgaactcat cttgtctccc aaagacgctc aggctccctt actcaaggat gcttatacct
     5701 ttcccggtct tttgccgtaa agtttttcta taagatcgac gcgtttcagg cgccggagtg
     5761 ctttcacaat agaagcttta tcctccgggc ggtaccaaaa gaaatattgc cgctgagcca
     5821 gcttttcttc cctggttttt gccgtataaa caggtttgag cgtgtaagga ttcagtcctg
     5881 tgtagtacat ttctgtggcc agggtcatgg gcgtaggggt aaagtcttgc acttgttcca
     5941 gtctaaaatt aagtgttttg gttttagcgg ccaactcggc catatcttct tcgctacagg
     6001 caggatgcga ggaaataaaa tagggtacaa gttcctgacg tcctttgatt ttactttcgc
     6061 ctttaggaga ttcagttgaa aggaggtttt ggtttttatg aggttcgttt gtgcgaaagt
     6121 tttgattttt tcgctgttcg ctcagacgac gattctcgat ttgttcaaag atttccctga
     6181 attgtatgaa ctgtgaaaac gggggcttgc gcatagaatt taatacgctg ttttcggtat
     6241 gttccggtgc tactttcagc cggccggata cgtgcttttc gatcagttga cgagtgtatt
     6301 cctgtgcggc tctgtctttg tctgcagcct ctttaatggc ggtttgattg cattcgcctg
     6361 attttaaagc ttgttgcgaa gagtaaagca ggaggtcgta gcggatgccg ctgcttatta
     6421 cacttttttt gatgccgggc agggcatcta ctgcttggta taagtcgagc aagggccggt
     6481 ggtctacgtt gaggttggaa cagattttcg ggaaaaggca ggaattacgt ttgcattttc
     6541 gacaaatctc ctggttttgt ccctgcatgc gatacatgtt ggccgaagga ccacccaaat
     6601 cgctcagata gcctttgaaa tcgcttcttt ggctgagcag gctcacttct ttcagtaccg
     6661 actccttgct gcgactcacc acaaatttac cctgatgggc ggcgatggca cagaaggagc
     6721 aaccgccgaa acaaccccgg tgcaggttga ccgaaaattt gatcatttcg taggccggaa
     6781 tgggcttacc tttgtatttg ggatggggca ggcgggtata gggtaactca aagctggcgt
     6841 cgagttcttc gctgctgagg ggtggatggg gcgggttaac cacaatttcc atttccccga
     6901 cgcgctgaat caggaaggaa gcctccattt tgttggattc ttcctcgatg acgcgaaaat
     6961 tttcggcctg tttgcgtttg ctgttcaggc attcttcgtg cgaatgcaat tgaatgcagg
     7021 aggatatttc cttgcctgct gtatcgcctg cttgcctcag gtaggcgact tgcctgagct
     7081 cgcgtagcgc ttcgaaggga gctcccgaga gcaaaagccc ggctatttct ttcaggggtt
     7141 tttcgcccat gccgtagacc agtaggtcgg ccttgctgtc gaccagaatg cccggcataa
     7201 ggcggtccga ccaataatcg taatgactga gtctgcgcat ggaggcttct atgcctccaa
     7261 tcactatggg aacttctgga tagagttttt taaggatgtt ggcgtaagtg atggtagtgt
     7321 agtcgggacg ggctcccggc aatccgtcgg ctgtataggc atcgtcggaa cgcagacgtt
     7381 tgttggcggt atagcggttg accatggaat ccatacaacc ggccgaaatg gcaaaaaagt
     7441 accgcggttt ccccattttg gtgaagtcgc gatgatctcc tctccagtcg ggctgcggca
     7501 cgagggccac tttcattccg caagcttcca gcagacgccc ggccacggcg gccccgaaag
     7561 aggggtggtc tacataagca tcgcccgaaa acaagacgac gtcggcttgt tcccagcccc
     7621 tgagttccat ttcttttttg gtaacgggca gccagtcggt ttttt
//
LOCUS       DAOSTD010000079         7433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_9299_length_7433_cov_13.140282, whole genome shotgun sequence.
ACCESSION   DAOSTD010000079 DAOSTD010000000
VERSION     DAOSTD010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9299_length_7433_cov_13.140282"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PLI37_06040"
     CDS             <1..668
                     /locus_tag="PLI37_06040"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOQ58603.1"
                     /translation="FKPHPMKRNSLFKDNYWLFIPYLLMLAISVFFILFYSKAEIHIW
                     MNQHHSPFADLLFKYASYMGEGWVLTPICLILLFVRFRYAFMAIGSSILAPLFTQFFK
                     RVVWPDSPRPKVFFENLYDLHVVDGVHLHSAHSFPSGHATGAFAVFMVLALIVKNPFW
                     KVFFLVLASLTAYSRVYLSQHFLVDVSVGSLIGSLTALLCYCWMMRYSKGWLDKSFKI
                     CVK"
     gene            complement(620..>1141)
                     /gene="yihA"
                     /locus_tag="PLI37_06045"
     CDS             complement(620..>1141)
                     /gene="yihA"
                     /locus_tag="PLI37_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOQ58604.1"
                     /translation="SNVGKSSLINMLLNQKNLAKTSSTPGKTLLINHFLVNEAWYLVD
                     LPGYGYARVSLKQREQLHRMISDYMLKRPNLQCVFVLLDSRHRPQEIDLDFLEFLGEN
                     QVPFALVFTKIDKCKKSELNLNIENYRNRLLESWESLPPLFLSSSEKKQGRTALLTYL
                     TQILKDLSSQPLE"
     assembly_gap    1142..1241
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1242..2134)
                     /locus_tag="PLI37_06050"
     CDS             complement(<1242..2134)
                     /locus_tag="PLI37_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008667157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOQ58605.1"
                     /translation="MNEFLVLGLLLAYFGLLLLISLLSRGSDQNNNAFFLGERKSPWP
                     VVAIGMIGASLSGVTFVSVPGMVRENSFLYMQTVMGFFVGYILIANVLLPVYYRLNSP
                     SIYVYLKERFGKYSYKTGAAFFLLSKTIGAAARLYVVAIVLQVFVLEKWGVPFALTVI
                     LIIAMIWMYSSRSGIKTIVWTDLLQTLCLLAALVLIILQLSKSLQPESGSVWGLMKAS
                     PYMKMFEFEDWRSRQHFVKQFFSGIFIALVMTGLDQDMMQKNLSIPKLDKAKKNIYTY
                     GFAFIPVNFLFLSLGVLLLLFA"
     gene            2222..2836
                     /gene="recR"
                     /locus_tag="PLI37_06055"
     CDS             2222..2836
                     /gene="recR"
                     /locus_tag="PLI37_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOQ58606.1"
                     /translation="MDQIYPSVLLEEAVQAFSGLPGIGRKTALRLVLHLLRQNKEDVE
                     HFGNTLIRLKNEIAYCKTCHNISDTEECLICSNPRRDTSIICVVENVREVMAIEQTRQ
                     YNGLYHVLGGLISPMDGIGPSDLEINSLVERLAQNRVEEVILALSATAEGDATSFYIF
                     RKISTYPLRITVIARGISVGDELEYADEITLGRSILNRIPFKTS"
     gene            2838..3341
                     /locus_tag="PLI37_06060"
     CDS             2838..3341
                     /locus_tag="PLI37_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58607.1"
                     /translation="MEADLHLVLKKLRLQFFLSVGLLLCLGLLAYLFPLQSILLSQEL
                     SLPFQSFCILLFLIGVPFSLRWFHHKTRAIRLEKALKERLPAYRKAASARMYVFSLIA
                     LFALFLEVFTTMPNALVFFLMTMLFFLFCIPVKGQVIKDLELFPNPEQAQETEEQPSE
                     QASERPK"
     gene            3353..3880
                     /locus_tag="PLI37_06065"
     CDS             3353..3880
                     /locus_tag="PLI37_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ58608.1"
                     /translation="MRTFLQNEQLRLRSPEPEDLEFLYRCENDTSLWQYGSTLSPFSK
                     YALHQYISQAEQNIFVSRQLRFMIDSKKDDCVVGTIDLYDFDPYHNRAGVGILIDKAY
                     QRRGYATQALKLLIEFAFGFLHIHQLYAYIPTENSDSLALFSKLGFEQSGVLKDWNMG
                     LDKYKDIAVFQLIAP"
     gene            complement(3948..4334)
                     /locus_tag="PLI37_06070"
     CDS             complement(3948..4334)
                     /locus_tag="PLI37_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013264577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOQ58609.1"
                     /translation="MNQSVRIDKWLWSARIYKTRTLATEACRKGQVSIAGVKVKPSRM
                     VTEGEILNVRKNPLTLTLKILSPIQNRVPARLIPEVLQDLTPAEEYEMLEMARIASFG
                     KRAAGSGRPTKKERRDLESFLNNEDE"
     gene            complement(4399..4962)
                     /gene="pth"
                     /locus_tag="PLI37_06075"
     CDS             complement(4399..4962)
                     /gene="pth"
                     /locus_tag="PLI37_06075"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOQ58610.1"
                     /translation="MKYLIAGLGNVGETYQHTRHNIGFDILDAFSEASNVVFEPKRYG
                     AVAEGRLKNKQFVLLKPSTYMNLSGNAVRYWLQAEKIPLENLLVVVDDLALPFGRMRL
                     RPDGGDAGHNGLSHIQESLGTDKYARLRFGIGNDFPQGTQVDYVLSGWTAEQLETLPE
                     RIDQAVEIIKSFCLAGIQLTMTRYNRK"
     gene            complement(4959..5561)
                     /locus_tag="PLI37_06080"
     CDS             complement(4959..5561)
                     /locus_tag="PLI37_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOQ58611.1"
                     /translation="MKTLELNGSPRTDFGKKASKNVRNKGNVPCVLYGGEKNLHFQVK
                     KEDLRALIYTPAVHLIKLNIAEKAYMSILQDVQFHPVSDEPLHLDFLQIFEDKPILIE
                     VPVKLNGFAAGVKDGGKLSLDLRKLKVKALYKDIPDFLEIDITNLELGKTVQVGDLEF
                     PNMELMNTKNAVVCAVKLTRVARGIAAATAATESEESETE"
     gene            complement(5777..6142)
                     /locus_tag="PLI37_06085"
     CDS             complement(5777..6142)
                     /locus_tag="PLI37_06085"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOQ58612.1"
                     /translation="MKTDTEDNKNIDKDMVYNKTVKAGKRIYYFDVKKSRNNEMYIAI
                     TESKKIVSGDLLTPDISFKKHKIFLYHEDMSNFAEALLQVIQYVKERQAPPPPREQYR
                     EKKKEADRSTPDEDDGLGI"
     gene            6286..7215
                     /gene="nusB"
                     /locus_tag="PLI37_06090"
     CDS             6286..7215
                     /gene="nusB"
                     /locus_tag="PLI37_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOQ58613.1"
                     /translation="MINRVLIRTKVLQMVYACHQNNNNENPAKAEKELFFSLEKTYDL
                     YLYLLLLVSDIGYYAERRIDMARGKYLPSPEEQNPNLRFVNNAFLAQLRQNTTFKREI
                     NNRKLSWAPHEGLIKNIYDQILMAPFYIEYMQAESNSYEADKEVWRKIFKQIIQNNSY
                     LEEVLEEKSIYWNDDLDIVLTFVLKTVKRFEQSNGANQALLPMYRDESDRQYALDLFR
                     ASIKNAEEYRSMITKAVQNWELERLALMDLIILQVALAEIMEIKDIPLSVSFNEYIDI
                     AKSYSTEKSSLFINGTLDHIVRELKKENKLFKV"
     gene            7332..>7433
                     /locus_tag="PLI37_06095"
     CDS             7332..>7433
                     /locus_tag="PLI37_06095"
                     /inference="COORDINATES: protein motif:HMM:NF014725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOQ58614.1"
                     /translation="MQFLPLILIFLVIWLFMIRPQNKKQKEIQKARAA"
BASE COUNT         1982 a         1694 c         1541 g         2116 t
ORIGIN      
        1 ggtttaaacc tcatcccatg aaacgaaact ccctgttcaa agacaattac tggctcttta
       61 tcccttattt gctgatgctc gctatcagcg tttttttcat ccttttttat tccaaggctg
      121 agatacatat ctggatgaac caacaccaca gcccctttgc cgatcttctc tttaaatacg
      181 cctcttacat gggcgaaggt tgggtactga cgcccatttg cttgatttta ttgtttgtac
      241 gctttcgtta cgccttcatg gccataggct ccagcatttt ggcccctttg tttacccaat
      301 ttttcaaacg ggtggtttgg cccgacagcc ccaggcctaa agtttttttc gagaacctct
      361 acgatttaca tgtggtcgat ggagtgcatt tacattcggc ccacagcttt ccctcgggac
      421 acgccacggg agcttttgcc gtttttatgg tactcgcttt gatcgttaaa aatccctttt
      481 ggaaagtatt ttttctggta ttggccagtc tgacggccta ttcgagggta tatttatccc
      541 agcatttttt ggtggacgtc agtgtgggtt ccctgatagg aagccttaca gcgctgctct
      601 gttattgttg gatgatgcgc tattccaaag gctggctgga taaatccttt aaaatctgcg
      661 ttaaataggt aagtaaggcc gttctaccct gttttttctc ggacgaactc aaaaaaagcg
      721 gaggcaggct ttcccagctt tcgagcaggc gattgcgata gttttcgatg ttgagattca
      781 actcggattt tttgcattta tctattttgg taaacaccag cgcaaaaggc acctggtttt
      841 ctcccaaaaa ctccaaaaaa tcaagatcga tttcctgtgg ccgatgcctc gaatcgagca
      901 aaacaaacac acactgcaaa ttgggtcttt tcagcatata atcgctgatc atgcgatgca
      961 actgctccct ttgtttgagg gaaaccctgg cgtaaccata accgggcaaa tccaccaaat
     1021 accaggcttc gttgaccaga aaatgattaa tcagcaaggt tttacccgga gtcgaggaag
     1081 tcttggccaa gtttttttga ttcagcaaca tattgatcag ggaagatttc cctacattgg
     1141 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcaaacagc aacaacaaga
     1261 cacccaggct cagaaataaa aaattcaccg gaataaaggc aaaaccgtag gtataaatat
     1321 ttttctttgc tttatcaagc ttgggtatac tcaggttttt ttgcatcatg tcctgatcca
     1381 aacccgtcat gaccaatgca atgaaaatcc cactgaaaaa ctgtttgacg aaatgctgcc
     1441 tgctgcgcca atcttcgaac tcaaacattt tcatgtaagg actggccttc atcagccccc
     1501 agaccgaacc gctttcgggc tgtaaactct tggacagttg aagaataatc aaaaccaggg
     1561 ctgccaataa acagagcgtt tgtagcaaat cggtccacac tatggtttta atgccgctac
     1621 gagaggaata catccagatc atcgctatga tgagaatgac cgtaagggca aagggcaccc
     1681 cccatttttc aaggacaaag acttgcaaaa caatggctac cacatacaga cgggcagccg
     1741 ctcctatggt tttggacaag agaaaaaagg cggctccggt tttataagaa tacttgccga
     1801 agcgctcttt cagatagaca taaatactgg gagaatttaa gcgataataa accggcagca
     1861 agacattggc aatcaaaata tagcccacga aaaaacccat cacggtctgc atgtacaaaa
     1921 agctgttttc gcgtaccatg cccggaaccg aaacaaaagt tactcccgaa agcgaagccc
     1981 ctatcatgcc tatggccacc accggccagg gcgacttgcg ttctcccaga aaaaaggcat
     2041 tgttgttttg gtcggatcct ctggacaaga gggatatcag caagagcaat ccaaaataag
     2101 ccagtagcag tcccagcaca agaaattcat tcataaactt ttgaataggc tgcaaaaata
     2161 cgggattatt tttaaaattg gggatttgaa cgtacttttg cccctcagtg ttcgatgtac
     2221 aatggatcaa atctaccctt ccgttttatt ggaagaagct gtgcaggctt tttccggctt
     2281 gcccggtata ggcaggaaaa ccgccctgcg tctggtgttg catcttttgc gtcaaaacaa
     2341 agaagatgtg gagcattttg gcaataccct gatacgcctg aaaaatgaaa tcgcctattg
     2401 caagacctgc cacaatattt ccgacaccga agaatgcctg atttgcagca atcccagacg
     2461 agatacctcc atcatttgcg tggtagaaaa tgtgcgcgaa gtaatggcca tagaacagac
     2521 caggcaatac aacggccttt accatgtgct gggcggcctg atatctccca tggacggcat
     2581 agggccttcc gatcttgaaa tcaattcatt ggtggaaagg ctcgcacaaa accgggtcga
     2641 agaagtcatt ctggcccttt cggccaccgc cgaaggcgat gccacctcct tttatatttt
     2701 tcgaaaaatc agtacgtatc ccctgaggat cactgttata gcccgaggaa tctcggtggg
     2761 tgacgaattg gaatatgccg atgaaatcac gctgggacgt tccatactca atcgaattcc
     2821 ttttaaaacc tcctgatatg gaagctgatc tccatcttgt gctgaaaaaa ctgcgcttac
     2881 aattttttct cagcgttggc ttactcctgt gtttaggcct gttggcttat ttgtttccac
     2941 tacagtctat cctgttgtcg caagagcttt cccttccttt tcaaagcttt tgcatcctat
     3001 tgtttttgat cggcgtgcct tttagcctgc gttggtttca ccacaaaacc agggcaatac
     3061 gcctcgaaaa agcgttgaaa gaacgtctgc cggcctaccg gaaagcggct tccgcaagaa
     3121 tgtacgtctt cagtttaatc gctctttttg ctctgtttct ggaagttttt accaccatgc
     3181 ccaacgcttt agtctttttc ttgatgacca tgctcttctt tttgttttgc attccggtca
     3241 aaggacaagt catcaaggat ttagagcttt ttccgaaccc ggagcaggct caggaaacgg
     3301 aggagcagcc ttcggagcaa gcttctgaga gacccaaata attcccccaa ccatgagaac
     3361 cttccttcaa aacgaacaat tgcgcttacg gtcgcccgag cccgaagacc tggaattcct
     3421 ctaccgctgc gaaaacgaca ccagcctctg gcaatacggc agcaccctgt cgcccttttc
     3481 aaaatatgct ctgcatcaat acataagcca ggccgaacaa aatattttcg tttccaggca
     3541 attgcgtttt atgatagaca gtaaaaaaga cgactgtgtt gtcggcacca tcgatcttta
     3601 cgattttgat ccctaccaca accgcgccgg tgtaggcatt ctgatagata aggcctatca
     3661 gcgcaggggc tatgccactc aggctttaaa gctactgatt gaatttgctt ttggttttct
     3721 gcatattcat cagctttacg cctacattcc taccgaaaac tcagacagcc tggctctttt
     3781 tagcaaactg ggttttgaac aaagcggtgt gctgaaagac tggaatatgg gactggataa
     3841 atacaaagat attgctgtat ttcaattaat tgcaccctaa attcagcttg ttgctcataa
     3901 ggtggggtct tatgagtacg ggaattctat ttgcttagat ttgccgttta ttcatcttca
     3961 ttatttagga aactttccag gtcgcgacgt tctttcttgg tgggcctgcc gctgcctgcc
     4021 gctcgcttgc caaagctagc aatacgggcc atttcgagca tttcgtattc ttcggcaggg
     4081 gtcagatcct gcagaacttc cggaattaat cttgcgggga ctctgttttg aatgggactc
     4141 agtattttca gcgtcagggt aagaggattt ttgcgtacgt tgagtatttc gccttcggtc
     4201 accattctgg agggctttac ttttacccct gcaatactca cctggccttt gcgacaagct
     4261 tcggtggcca gtgttctggt cttgtagatc cgggcagacc acagccattt gtctatgcgt
     4321 acgctttgat tcatgtttat ttacggttcg ctggtgcagc aagccaaacg tttcgggctg
     4381 ggaggcttcg atccatgctt atttgcggtt gtacctggtc atggtaagct gaatgccggc
     4441 cagacaaaag cttttgatga tttctacggc ctgatctatc ctctcgggca atgtttccag
     4501 ttgttcggca gtccagccgc tgagcacgta atccacctga gtcccctgag ggaaatcgtt
     4561 gcctatacca aaacgcaggc gggcgtattt atcggtgccc aggctttcct gtatatgact
     4621 cagtccgtta tggccggcat ctcccccgtc gggacgcaaa cgcatacgac caaaaggcaa
     4681 tgccagatcg tctaccacta ccagcaaatt ctccaagggg attttttcgg cctgcagcca
     4741 gtaacgcacg gcgttaccgc tgagattcat gtaagtagaa ggtttcagca aaacgaactg
     4801 cttgttcttg agccggcctt ctgccacagc tccgtaacgc ttaggctcaa aaacaacatt
     4861 ggacgcttcc gagaaagcgt ccaatatgtc aaatccgatg ttgtgccgag tatgttgata
     4921 ggtctcacct acgttcccca aaccggcaat caggtatttc attctgtttc gctttcttcc
     4981 gattctgtgg cagcagtggc agcagctata cctctggcta ctcgggtcag tttaacagca
     5041 caaaccacgg cgtttttggt gttcatcagt tccatgttgg gaaattcgag atcacctacc
     5101 tgtaccgttt tacccaactc taagttggta atgtcgattt ccagaaaatc gggaatatct
     5161 ttatacaaag ctttcacttt gagtttgcgc aaatccaggc tcaacttacc gccatcctta
     5221 acaccggccg caaaaccatt gagtttgaca ggtacttcaa taaggatggg tttatcttcg
     5281 aaaatttgca agaaatcaag atgcagaggc tcatcactga cgggatgaaa ctgtacgtcc
     5341 tgaagtatcg acatataagc cttttctgca atattaagct ttatcaaatg aactgcagga
     5401 gtataaatca atgctcgtaa atcttctttt tttacctgga aatgcaggtt tttttcacct
     5461 ccgtaaagga cacaaggtac gttgccttta ttgcgaacgt ttttggaggc ttttttcccg
     5521 aaatcggttc tcgggctgcc attaagttct aaagttttca ttgtgattga attgtgttac
     5581 tcaaaaattt aaaataagct cttaaatttt cccatcacac gggtttagtc aaaagcggcg
     5641 caaaggtaat caaaagattt attacaacaa acaagcctga aaaattatta gctcgaatcg
     5701 ccggaatctt cgtagttatc gtctttgtcg gcagtagtcc ggtcgctgaa ttcttcaaag
     5761 ctcttgacaa acttgcctaa atccctaagc cgtcgtcctc gtcgggggtg ctgcgatcgg
     5821 cctctttctt tttctcccga tattgttccc ggggcggagg tggtgcttgt ctttctttga
     5881 cgtattgaat tacctgaagc agagcttcgg caaaattgga catgtcttcg tgataaagaa
     5941 atattttatg ctttttgaag ctgatatccg gggtaagtaa atcaccgctt acaatctttt
     6001 tactttcggt aatcgcaata tacatctcgt tgtttcggct tttttttaca tcaaaataat
     6061 agatgcgttt tcctgctttt accgttttgt tatagaccat gtccttgtct atattcttat
     6121 tatcttctgt gtctgttttc atggtacttc atcaataaat tcgataataa gttcaaccgg
     6181 agttgtcttt gatctgagtt caaaattaga tataatattt taaggatgca atcttgcttg
     6241 gtaaaatata ttatctttgc ggcgttgctt tttgtagaat ccgtaatgat caatcgtgtt
     6301 cttatccgta ctaaggtatt acaaatggtg tatgcctgcc accagaacaa taacaacgaa
     6361 aacccggcaa aagccgaaaa ggaattattt tttagtttag aaaaaaccta cgatctgtat
     6421 ttatatctat tgttgctggt ctcagacatc ggttattatg ccgaacgacg catcgacatg
     6481 gctcgcggca aatacctgcc cagcccggaa gaacaaaatc cgaatttgcg ttttgtgaac
     6541 aatgcctttc tggctcaact caggcaaaac accaccttca aacgcgagat aaacaaccga
     6601 aaattatcct gggcacccca cgaaggattg ataaaaaata tttatgacca gattctaatg
     6661 gctccttttt atattgaata tatgcaagcc gagagcaaca gctacgaagc cgacaaagaa
     6721 gtctggcgca aaatatttaa acaaattatc caaaacaata gttatctgga agaagtattg
     6781 gaagagaaat ctatctattg gaacgacgat ttggacatag tccttacctt tgttctgaaa
     6841 accgtcaagc gttttgagca aagcaatggc gcaaaccagg cacttttgcc catgtacaga
     6901 gacgaaagcg atcgtcagta tgcgctcgac ttattcagag cttccataaa aaatgccgaa
     6961 gaatacagaa gcatgattac caaagctgtt caaaactggg aactggaacg cctggccctt
     7021 atggacttaa ttatactgca ggtggccctg gccgagatta tggaaataaa ggatattccc
     7081 ttaagcgtca gttttaatga atatatagac attgctaaaa gctacagtac agaaaaaagt
     7141 agtttattta tcaacggaac actcgatcac atagtccggg aactaaaaaa agaaaacaaa
     7201 ttatttaaag tctgattttt caattattag ttattagtta tataatctct atcgttatga
     7261 atttattcat tttattacaa gctgcccagg caggtgctga aacagctgcc gaagccccta
     7321 gaagtggttt tatgcaattt ttgccactta ttttgatttt tctggttatc tggttgttta
     7381 tgatccgccc gcaaaacaag aaacaaaaag aaattcaaaa agcccgtgcc gcc
//
LOCUS       DAOSTD010000080         7431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_9303_length_7431_cov_12.481697, whole genome shotgun sequence.
ACCESSION   DAOSTD010000080 DAOSTD010000000
VERSION     DAOSTD010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9303_length_7431_cov_12.481697"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..599
                     /gene="trmD"
                     /locus_tag="PLI37_06100"
     CDS             6..599
                     /gene="trmD"
                     /locus_tag="PLI37_06100"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOQ58615.1"
                     /translation="MVDINVHCLRDYSTDLKHRRVDDYAFGFSAGMVLQIEPVDRVIT
                     HLCSQRKYDEIIYTSPDGEKFTQRQANQLSLKQNLIVLCGHYKGIDHRIREHLITKEI
                     SIGDYVLTGGELAAAVITDCVVRLIPGALGDEQSALSDSFQDQLLAPPIYTRPAEYKG
                     WKVPDILLSGNQRKIQEWEMEQSLERTQRLRPDLLDD"
     gene            complement(750..1664)
                     /locus_tag="PLI37_06105"
     CDS             complement(750..1664)
                     /locus_tag="PLI37_06105"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOQ58616.1"
                     /translation="MVDLSVQLKALRLKNPVMTASGTFGYGEEYLDFMDVSRIGGIIV
                     KGTTIRPREGNDYPRMAETASGMLNAVGLQNKGVHYFVKHIYPRICDFNTNVLVNVSG
                     STVDDYVQAAEIIQELDKIPGIELNVSCPNVKEGGMAFGVSPRALSLVVKEVRAVYKK
                     HLMLKLSPNVTDITELAKIAEDQGADSLSLINTLLGMAIDAENRRPKLSTVTGGLSGP
                     CIKPVALRMVWQTYKAVKIPVIGIGGISNTADAIEFMLAGASAIQVGTANFIDPGVSV
                     SIAEGITDYCRRHGFKSAKELTGSLIID"
     gene            complement(1658..2449)
                     /locus_tag="PLI37_06110"
     CDS             complement(1658..2449)
                     /locus_tag="PLI37_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007368293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOQ58617.1"
                     /translation="MSSIINKQVLDFILYEKEEWANSALMKFKTTEVLPEIFPGQFVQ
                     VKIENSPDTYLRRPLSVNFTDRENSCLWLLVQKIGPGTRKLAGLALNSKVNMILPLGK
                     SFTLPEPAKDSFQQQIMLIGGGIGTAPLLFLGKSLHERSYSPVFLLGGRSAADILQID
                     QFRLYGKVYVTTQDGSMGERGLVTEHSILKEMQPDRMYCCGPKPMMQAVASLARAKNC
                     WCEVSLENLMACGFGACLCCVEKTTQGHVCTCTEGPVFNINQLLW"
     gene            complement(2534..2977)
                     /locus_tag="PLI37_06115"
     CDS             complement(2534..2977)
                     /locus_tag="PLI37_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ58618.1"
                     /translation="MKDRIKEILDSQGLNATQFAQKVNINVSALSHILSGRNNPSYDA
                     LQKIIVNFPGINVEWLMTGQGEMYKENLPIQQEEVTLFGENPQKPDHDSGKEGYLPQK
                     GLKSSQKPSMYTDNQTNTATEKTTAKISKIAVFYSDNTYEEFFPQ"
     gene            3799..4500
                     /locus_tag="PLI37_06120"
     CDS             3799..4500
                     /locus_tag="PLI37_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOQ58619.1"
                     /translation="MDIKSILDFLHLLSQNNNREWFNKHKAFYTTSREHHIRLLEKLI
                     LLLADFDAELRHLRPQECIFRIYRDLRFTQDKTPYKTFFGAFMAAGGKNSERAGYYLH
                     LKPDNCFITGGIWMPEAPQLKILRQSIVDNLDEFEDIVHSQDFVSSFGSLDGRRLRTA
                     PKGFPADFPGMDYLKFKDYTATHKLSALEMERSDFCEYIAGLFQHLYPLNRFLNFALD
                     EYKENKPFYNHPINS"
     gene            4503..4949
                     /locus_tag="PLI37_06125"
     CDS             4503..4949
                     /locus_tag="PLI37_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOQ58620.1"
                     /translation="MILDSLKNSSLYESMHPLFKRAFDYLKSRDLDLLEPGKIVLDGD
                     KLYVSISEYEGKSPEAARLETHEKYIDIQVIISGTETMGWRAANDCKHALAPYDTVKD
                     ICFYKDNPVSFVEVHPGEFTVFFPGDGHAPCIGKGPIKKAVVKVLL"
     gene            4949..6946
                     /locus_tag="PLI37_06130"
     CDS             4949..6946
                     /locus_tag="PLI37_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOQ58621.1"
                     /translation="MKSKTLKIIKNDPWLEPYRQAIEGRYNYALSREKSLTKGGSLSE
                     FANGHLFFGLHKHPDGWTFREWAPNADSIYLIGEFNQWERRPEYALEKKEHGVWEIHL
                     AADCLKHGDLYKMYVSWPGGSGERIPAWTRRVVQDPVSLIFSAQVWEPANPYRFKHKV
                     KPPSQPLFIYECHIGMATEEERIGTYDEFRRLVLPRIVDNGYNAIQIMAIQEHPYYGS
                     FGYHVSSFFAASSRFGTPEELKRLIDEAHGYGLAVIMDIVHSHAVKNSVEGLGCLDGD
                     PNLYFHSGDRREHPAWDSLCFNYGRDEVLHFLLSNCKYWLDEFRFDGFRFDGVTSMLY
                     RSHGLGESFSSYDDYYNLNQDGDAIAYLTLANKLIHEIKPKAITIAEEVSGMPGLAAP
                     IKDGGIGFDYRMAMGIPDFWIKLIKEYSDEDWKPGAIWWELTNRRKDEKTISYAESHD
                     QALVGDKTIIFRLIDADMYWHMSKNDQNLVVDRGIALHKIIRLLTASTINGGYLNFMG
                     NEFGHPEWIDFPREGNGWSYKYARRQWSLLENRQLKYADLHAFDKAMVLLLKKINPKE
                     PLYQRWLHDQDQIMAFERASFLFVFNLHPSRSFVDYGILVQKGNYKIVLNSDAKEFGG
                     FGLVDEQVVHTTTQTAKNPDFELEWIRLYLPSRTALVLKRQ"
     gene            complement(7028..>7431)
                     /locus_tag="PLI37_06135"
     CDS             complement(7028..>7431)
                     /locus_tag="PLI37_06135"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOQ58622.1"
                     /translation="QETNDLTKAVNYCERAGYRQIILMGATGIREDHTLGNISLLVDY
                     SLRIEDICMQTNYGRIDVVRHYKQLQSYPGQQISLFAIDPSTIVNAEGLKYPLIDRRL
                     TNWWQGSLNEALGSTFEIRTQKGAVLVYRTD"
BASE COUNT         2097 a         1672 c         1523 g         2139 t
ORIGIN      
        1 agaaactggt tgacatcaat gtacattgtt tacgtgatta ttccaccgat ctaaagcatc
       61 gcagggttga cgactatgct ttcggttttt cagcaggcat ggtattgcaa attgaaccgg
      121 tagaccgggt catcactcat ctgtgtagtc aacgcaaata cgatgagatc atatatactt
      181 cgcccgacgg agaaaaattc actcaaagac aggccaatca actttccctg aaacagaatt
      241 tgatcgtcct ctgcggacat tacaaaggca tcgaccatcg catacgtgaa catctgataa
      301 ccaaagaaat atctattggc gattatgtgc tcaccggtgg agaactggca gccgccgtaa
      361 taacggattg cgtcgtgagg cttatcccgg gagctttggg tgacgaacaa tctgccctct
      421 cagattcctt ccaggaccaa ttgctggcac cgcctattta taccaggcca gcagaataca
      481 aaggctggaa agtcccagac atattattgt cgggaaacca acgtaaaatt caggaatggg
      541 aaatggagca atctttggaa agaactcaac gtctgcggcc ggatttgctt gacgattgat
      601 ttttgagttt cgctaaagta ttgctgttca acgtacacct agtcgtttca gcctacccgc
      661 tattggtttg catcaatcaa gttaaagccg ccttgagcga cgccatgaaa acaattccgt
      721 cagccctcat gcctacaatt tcttgtgtac taatcaatta tgagggatcc agtcagttct
      781 ttggccgatt taaaaccatg tcgccgacaa taatccgtta tgccttcggc tatgctgacc
      841 gagactcccg gatctataaa attagcggtt cccacttgta tggcagatgc accggccaac
      901 ataaattcta tggcgtcggc tgtgtttgag atacctccta tgcctataac agggattttg
      961 accgccttat atgtttgcca aaccattcta agagcaacag gcttgatgca gggtcctgac
     1021 aaacctcccg tcaccgtgga aagcttagga cgtctatttt ctgcatcaat ggccatacca
     1081 agcaaagtat taatcaaaga aagagaatcg gctccctggt cttcggctat tttggcgagc
     1141 tctgtgatat cggttacgtt gggcgataat ttcaacatca gatgcttttt ataaactgcc
     1201 ctgacttctt tgactaccaa agataaggct cgaggactca caccaaatgc cattccgcct
     1261 tctttgacat tggggcagga cacattgagt tctatacccg gaatcttgtc caattcttgt
     1321 atgatttcgg ccgcctgaac ataatcatcc accgtagaac cagaaacatt aaccagtaca
     1381 tttgtattaa aatcgcaaat gcgcggatat atgtgtttta caaaataatg cacaccctta
     1441 ttttgcagtc ctacagcatt taacatacct gaagctgttt cggccatcct aggataatcg
     1501 ttgccttctc ttgggcgaat agtagttccc tttacaatta tgcctcctat tcgcgaaaca
     1561 tccataaagt caaggtattc ttctccgtaa ccaaaagtac ccgaagcagt catcacggga
     1621 tttttcaggc gcagagcctt taattgtact gacagatcta ccatagcagt tgatttatat
     1681 taaaaaccgg accttcggtg caagtgcaaa cgtggccttg cgttgttttt tccacgcaac
     1741 agaggcaagc tccaaatccg catgccatta gattttcaag ggagacttcg caccaacaat
     1801 tcttcgctct ggctagggaa gctactgctt gcatcattgg ttttggaccg cagcaataca
     1861 ttctgtccgg ctgcatctcc ttcaggatac tgtgttccgt caccagtcca cgttctccca
     1921 tagatccatc ctgggtagtt acatatacct tcccgtaaag ccgaaactga tctatctgca
     1981 aaatatcagc cgcggagcgt cctcccaata aaaaaacggg agaataactt ctttcgtgca
     2041 agcttttacc taaaaaaagc agtggagcgg tgccaatacc cccacctatc agcattattt
     2101 gttgttggaa agaatcctta gcaggctcag gtaaagtaaa agacttgccc aggggaagta
     2161 tcatattcac cttggaattc aaagcaaggc ctgctaattt acgggtcccc ggaccgattt
     2221 tttgcaccaa taaccacaga caggagtttt ctctgtctgt gaaattcacc gataagggtc
     2281 gccttaaata ggtgtcggga gaattttcta ttttaacctg tacaaattgt cccggaaaga
     2341 tttccggcaa aacttcggtt gttttaaact tcatcaaagc cgaattggcc cattcttctt
     2401 tttcgtataa gataaaatct aaaacttgct tgtttataat cgaagacata attaggagtt
     2461 gaatacgcaa atatacgtga gaaatttcat ttcaggccta tccgattttt aaactgtact
     2521 ccaccaagtt taattattgt ggaaaaaatt cctcgtaggt gttgtcggaa taaaaaacgg
     2581 ctatcttgga tattttagca gttgtttttt ctgtagctgt atttgtctga ttatctgtat
     2641 acatgctagg tttttgtgag ctttttaagc ctttttgagg gagatatcct tctttacccg
     2701 agtcatgatc cggtttttgg ggattctcgc caaataaagt gacttcttcc tgctgtatgg
     2761 gcaagttttc tttatacatt tccccctggc cggtcatcaa ccactcaaca ttgattccgg
     2821 gaaaattcac aataatcttt tgtagagcat cgtagcttgg attattcctt ccgctcaaaa
     2881 tatgagataa ggcagatacg tttatattga ccttttgcgc aaattgtgta gcattcaagc
     2941 cctgggaatc taaaatttcc ttaattctat ctttcataaa ctggatatat atacattttt
     3001 ctgttgcaca aatgtacaaa taattgtctg gaaaaatctt tccaatcaaa aatattttac
     3061 agaaacaaat ctgcctatag ccacattaat aaaattattt ttatgtgatt tctgttatgt
     3121 aaagaccgtt ttacatattt gtttttacaa tactatatta attaattatg agcctattgc
     3181 tctaaatgaa gaatcttact cctttatacc cagcattata atgatcttat tttaaatcta
     3241 tacttttaac ttatctgctc aaagtcgttt tttgccctta ttatgtttgt attcgatctt
     3301 aacatacgta gtacattgtt atattccaat agtttgattt gatttaatta aagttcttac
     3361 tttaaatgtt ttcaggctta aactccatga ttgcttaaga atatgagttt acttttccaa
     3421 agtccccaca aatgtaaacc actcctctaa agagctacct gttttactta tgtgaatagc
     3481 cataaacaaa tgtttgtaat acacttatgt aaaccaattc tcctaaaaga atactctctg
     3541 atctatgctg attatatgca ttcctactcc ctatctgtcc tttttaaaag tagaactgtt
     3601 aataatgcct tactttttat ctcggatgag caaatctacc cttttttaga aaaagctgat
     3661 tttcgcctta agctctgaga atcctctgta cgtgtacaaa tggacgatcg gtttcaaata
     3721 aacgattctc aagaatcgaa aaaaacggta tgattgagtt ttttttccta catttggcaa
     3781 agaataaaac tctcgtaaat ggatattaaa agcatactgg acttcctgca cctcttatcg
     3841 caaaacaaca acagggaatg gttcaataaa cacaaagctt tttataccac aagccgggag
     3901 catcatatac ggcttctcga aaaacttatc ttgctgctgg ctgattttga tgctgaatta
     3961 aggcatttaa ggcctcagga atgcattttc aggatatatc gggacttacg cttcactcag
     4021 gataaaacac cctacaaaac tttttttgga gcttttatgg ccgcgggcgg aaagaatagt
     4081 gagcgggcgg gatattacct gcacctgaaa cctgataact gtttcataac cggaggcata
     4141 tggatgccgg aggctcctca gctgaaaatt ttacgtcaat ctatcgtaga caatctggat
     4201 gaatttgaag atattgttca ttctcaagac tttgtatcta gttttggttc tttggacgga
     4261 cgaagacttc ggacggctcc caaaggattt cccgccgatt tcccgggtat ggactatttg
     4321 aaattcaaag attataccgc cactcacaag cttagcgccc tggaaatgga acgctccgat
     4381 ttttgtgaat acatcgccgg tctttttcaa cacctctacc ctttgaaccg ttttttgaat
     4441 tttgcgttgg acgaatacaa agagaacaaa ccattttaca accacccaat aaatagttaa
     4501 acatgatact tgattcatta aaaaacagct ctctttacga aagcatgcat cctttattca
     4561 aaagagcttt cgattacctg aaatcacgcg acctggactt actggagccg ggtaagatag
     4621 tattggacgg agataaactt tatgtttcga tttcggaata tgaagggaaa tcacccgaag
     4681 cagcacgcct ggagacccac gaaaaataca tagacattca ggtcatcatc agcggcaccg
     4741 aaaccatggg ctggcgagcc gcgaacgatt gcaaacacgc tcttgctccc tacgatacag
     4801 tgaaagatat ctgtttttac aaagataatc cggtaagttt cgtcgaggtt catccgggag
     4861 aattcacggt gtttttcccc ggtgacggac acgcaccctg cataggcaaa ggccccatca
     4921 aaaaagccgt ggttaaagtt ttgctttaat gaaatccaag acattgaaaa ttatcaagaa
     4981 cgatccttgg ttggaaccct accgacaagc catcgaggga cgctataatt acgctctgag
     5041 cagggagaaa agcctgacca agggtggaag cctgtccgaa tttgctaatg ggcacctttt
     5101 ttttgggctg cataaacatc cagacggatg gacttttcgg gaatgggctc ccaacgccga
     5161 ttccatttat ttgattggcg aattcaacca gtgggagcgt cgacccgaat acgctctgga
     5221 gaaaaaggaa catggtgtat gggaaatcca tctagccgcc gattgtctga agcacggaga
     5281 cctgtacaaa atgtatgtct cctggcctgg cggtagcgga gagcggatac ccgcctggac
     5341 aagacgggtt gttcaggatc cggttagcct tattttcagc gctcaggtgt gggagccggc
     5401 aaatccttat cgctttaaac ataaagtaaa gcctccctcc caacctctgt ttatttacga
     5461 atgccacatt ggcatggcca ccgaagagga gagaataggc acgtacgacg agtttagacg
     5521 cctggtccta ccgcgcatag tcgataacgg ctacaacgcc attcagatta tggccataca
     5581 ggaacatccc tattacggtt ctttcggcta tcatgtttca agtttctttg cagcctcttc
     5641 ccgttttggc acacccgaag agctcaaacg gcttattgat gaggctcacg gctatggtct
     5701 ggcagttata atggatatag tacattctca tgccgtcaaa aattccgtcg aaggcttagg
     5761 ttgcctggac ggtgacccca acctgtactt tcacagcggc gacagaagag aacatccggc
     5821 ttgggattcc ctttgtttca attatggccg cgacgaagta ttacactttt tgctgtccaa
     5881 ttgtaaatat tggcttgatg aattccgttt tgacggtttc cgctttgacg gagttacttc
     5941 tatgctctat cgcagccacg ggctgggaga atccttcagt tcctacgacg actattataa
     6001 cctgaatcaa gacggagacg ccatagctta cctgactttg gccaataaac tcattcacga
     6061 aatcaaaccc aaagccatta ccattgccga agaagtcagc ggtatgcccg gtctggcggc
     6121 ccccattaaa gatggcggaa taggttttga ctatcgcatg gccatgggta tccccgactt
     6181 ctggatcaaa ctgataaagg aatacagcga cgaagactgg aagcccggag ccatctggtg
     6241 ggagttgacc aatcgcagaa aagacgaaaa aaccatcagt tatgccgaaa gccacgatca
     6301 ggctttggtg ggcgacaaaa ccatcatatt ccggctgatt gatgccgata tgtactggca
     6361 tatgagcaaa aatgaccaaa atctggtggt tgaccggggc attgctctgc acaaaatcat
     6421 acgtttattg acagccagca ccataaatgg aggctatctg aactttatgg gtaatgaatt
     6481 cggacatccc gaatggatag attttccgag agagggtaac ggttggtcct ataaatatgc
     6541 ccgtcgccaa tggagcctgc tcgaaaatcg tcaacttaaa tacgctgatt tacacgcttt
     6601 tgacaaagct atggttctgt tgctgaagaa aatcaatcct aaagagccac tctaccaacg
     6661 ttggcttcac gaccaggatc agattatggc cttcgagcga gcctctttcc tatttgtctt
     6721 caatttgcat ccttccaggt ccttcgtcga ttacggcatc ctggtgcaaa aaggcaacta
     6781 taaaatcgtc ttaaacagcg atgccaaaga attcggaggt tttgggcttg tcgatgagca
     6841 agttgttcat actacaaccc agacggcaaa aaatcctgac ttcgagctgg aatggatacg
     6901 tctataccta ccttccagaa cagctttggt tctgaaaagg caatgatttt tttcattggc
     6961 aatgggcaag atttaacgcg aatatagtta gccggctgaa taaagtcaga aaacgggctt
     7021 gaacaaatca atcggtacgg taaaccaata ctgccccctt ttgagtccga atttcgaatg
     7081 tggaacccag ggcttcgttg agacttccct gccaccaatt agtcagcctg cgatcgatca
     7141 gaggatactt caatccttct gcgttgacta ttgttgacgg atcgattgca aagagggaga
     7201 tttgctggcc ggggtagctc tgcagttgct tgtaatgcct gacgacatct atgcgaccat
     7261 agttcgtttg catacagata tcctctattc gcaaagagta atccactaat aatgagatgt
     7321 ttcccaaagt atgatcttct cttataccgg tagctcccat aaggataatt tgtcggtaac
     7381 cggcacgttc acaatagttc accgctttgg tcaaatcgtt ggtttcctgc t
//
LOCUS       DAOSTD010000081         7391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_9362_length_7391_cov_13.766494, whole genome shotgun sequence.
ACCESSION   DAOSTD010000081 DAOSTD010000000
VERSION     DAOSTD010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7391
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9362_length_7391_cov_13.766494"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /locus_tag="PLI37_06140"
     CDS             complement(<1..1555)
                     /locus_tag="PLI37_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOQ58623.1"
                     /translation="MKRQIYAFKIEFQALNFKLRFLLLLLFFFVLESNGQNPFFKASE
                     LSQTWVYYYPEHWPENQWERDFKNMAGLGFEAVHMGEFAWAQLEPEEGRYHFEWLDRA
                     IDLADKYQLKVILCTSTATPPVWMSRKYPEILIKHENGNHSDHGARQHASFSSPKYRE
                     LALRTIEELAKRYGSDTRVIGWQLDNEPSVQYDYNQAAAAEFRIFLQNKYQTIDKLNQ
                     AWGTAFWSQLYTSFDQINIPLMKQQFMNHHQILDYMRFAAQQTASFLDMQSRCLKKHI
                     IVQQFVTTNYIPNYEEGHIGLCKELDFHSYTRYMVFGENKGLGRKGFRVGEPLRIALA
                     NDFFRPIDNLYGVMELQPGQVNWGSVNSQPLPGAVRLWLWSVFSGGSRFVCTYRYRQP
                     LYGTEQYHYGIVGTDGLSLSSGGKEFCTYMEELELLRKQYDRPRGELDYLSKAAILYN
                     HENAWSIRNQKQNQHWNTEKHILSYYSALKSFGSKVDVITESADFEHYPLIIAPAYQL
                     IDDELVERWK"
     gene            1689..2591
                     /locus_tag="PLI37_06145"
     CDS             1689..2591
                     /locus_tag="PLI37_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOQ58624.1"
                     /translation="MSEDNHFNYLTVNETDQKWGLYVTTCGFQSIKPHSSINPYLNKH
                     LSSYAFNTKQGRVLDEFQLLFVVKGSGFFQSASVPKTNIETGSMIMLFPQEWHNYSFS
                     KETGWDEFWVGFSGEHARSIVHQGFFRKEEPIFKLGINDEFVSLYRQLISYAEQENQA
                     YQQIISGIVRHMLGLLYYKSRNVVVSNKSVVDKINRARIMMRENVSSPQAPEEIASQL
                     GVSYSWFRKAFKKYTGISPAQFQMNLRLQHAKDLLMNSDKSITEIAYELNFESTGQFS
                     TFFKHKTGTTPREFRSVASAPGPY"
     gene            complement(2679..3950)
                     /locus_tag="PLI37_06150"
     CDS             complement(2679..3950)
                     /locus_tag="PLI37_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOQ58625.1"
                     /translation="MARPLAERMRPLTLDDYAGQKHLVGEGAILRKMIDSGTLSSFIL
                     WGPPGVGKTTLASIIAYKLECPFYSLSAVNAGVKDVRELIETAKNNRLFQQKPPILFI
                     DEIHRFSKSQQDSLLAAVEKGIVTLIGATTENPSFEVVTPLLSRCQVYVLKPLEEQDL
                     QELLDRALSKDPELKKRRILVEESGQLFRLAGGDARKLFNIIELLTQYQPEDPVIIND
                     ENVLASVQANPLAYDKDGEMHYDIVSAFIKSIRGSDPDAAVYWLARMVEGGEDPKFIA
                     RRMLISASEDIGLANPNALLLANACFDAIHKIGWPESRIILSETAIYLASSPKSNSAY
                     LAIEKASSMVRQTGNVSVPLHLRNAPTQLMRDLDYGKDYKYAHDYRDNFVEQDYLPPE
                     IKGKSIWEPQQNAAENRLKEFLKRLWKNRFQ"
     gene            4093..5172
                     /gene="serC"
                     /locus_tag="PLI37_06155"
     CDS             4093..5172
                     /gene="serC"
                     /locus_tag="PLI37_06155"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOQ58626.1"
                     /translation="MKKHNFYAGPSILSEYTIRQTADAILNFEGTGLSLMEISHRSKQ
                     FVAVCDQARALIKELLHVPEDFEVVFLGGGASLQFAMVPFNFLETKAAYLDSGYWASK
                     AIKEAKLFGEVQIVGSSKDDNYSHLPKNYQVPSDADYFYFCSNNTIYGTELHEDPHPG
                     VPVIADMSSDIFSRPIDFSSYGLIFAGAQKNLAPSGVTLVIVRKSMLDKVSRPIPTIL
                     NYKTHVENESMFNTPPVVPIYAALQTLLRYKELGGIEVIHDMNKQKAALLYEEIDTNP
                     LFKGTVTCKEDRSLMNVCFVMSEGYEHLEDEFDKYASEAGMVGIKGHRSVGGFRASIY
                     NAMPIASVQALVDTMRSFAQLKTQK"
     gene            5175..6095
                     /locus_tag="PLI37_06160"
     CDS             5175..6095
                     /locus_tag="PLI37_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ58627.1"
                     /translation="MKILVATEKPFAPVAVEGIREIIQGAGFGFELLEKYTDKNSLIE
                     VVSQADGVIIRSDIIDRQVIEAAGKLKIVVRAGAGYDNVDLEAASEKGICVMNTPGQN
                     ANAVAELAMGMMVYAVRNMFNGTSGTELKGKALGIHAYGNVGRNVARIAKGFGMEIYA
                     YDPYCKPEVIERDGVKVLTSVEDLYKKCQIVSLHIPATEETSKSINADLLGRMPKNAV
                     LVNTARKEVIDETSLMMLMENRIDFKYITDIMPAADAKFAEKFAGRYYSTPKKMGAQT
                     AEANINAGIAAAKQIVDFFKEGNQTFRVNP"
     gene            6102..7349
                     /locus_tag="PLI37_06165"
     CDS             6102..7349
                     /locus_tag="PLI37_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOQ58628.1"
                     /translation="MVKIRAFKGWRPPRNLVEKVASRPYDVLNSEEARQEAANNEYSL
                     YRIIKPEIDFPPGTDEHEPKVYTKAKENFDLFRKKGWLVQDNKAMYYIYAQTMNNRTQ
                     YGLVVCASVEDYMNGLIKKHELTRRDKEEDRMKHVRVNNANIEPVFLAYPAQEALDKL
                     VAGHIRKEPVYRFTAPDGFGHQFWLIDDDESIGTITRCFEQIPALYIADGHHRTAAAA
                     LVGNEKKEQNPHHNGQEEYNFFLAVCFPDNQLQIIDYNRVVKDLNGHDKQSFLAALEE
                     DFIVESKGSQIYKPSRLHNFGLYIDREWYSLTAKPGRYDDKDPLGVLDVNISSRLILD
                     RILGIKDLRSDKRIDFVGGIRGLKELERRVDSGEMALALALYPVSMKQLIDIADSGNI
                     MPPKTTWFEPKLRSGLVIHTLDD"
BASE COUNT         1989 a         1665 c         1763 g         1974 t
ORIGIN      
        1 ttttccagcg ttccacaagt tcgtcgtcaa ttaactggta tgccggagct attatcagag
       61 gatagtgttc gaaatcggcc gattcggtga tgacatctac tttggaacca aacgatttca
      121 gggcactgta atagcttaaa atatgttttt ccgtgttcca gtgttgattt tgtttttgat
      181 tgcggatgct ccaggcgttt tcgtgattgt acagtatggc cgctttcgag agataatcaa
      241 gctctccgcg tggccggtcg tattgcttgc gaagcagttc cagttcctcc atataagtgc
      301 aaaattcttt tcctccgctg ctcaggctca ggccatcggt accaacaatg ccgtagtggt
      361 attgctccgt tccgtacaaa ggttggcggt aacgataagt acacacgaac cggctacctc
      421 ccgaaaagac actccataac cacaaacgca ccgctccggg caagggctga ctgttgacac
      481 tgccccagtt tacctggccg ggttggagct ccatcacgcc gtaaagattg tcgataggcc
      541 ggaagaagtc gttggcaagg gcaatgcgca ggggctctcc cacacgaaaa cctttgcggc
      601 ccagaccttt gttttcgcca aagaccatat aacgggtgta ggaatggaaa tccaactcct
      661 tacacaaacc aatatggcct tcttcgtaat tgggaatata attggtggtt acaaattgct
      721 gtacaatgat gtgctttttc agacagcggc tttgcatatc caaaaaggag gccgtctgtt
      781 gagcggcaaa acgcatataa tccaggatct gatgatggtt cataaactgt tgtttcatca
      841 gggggatgtt gatctggtcg aaactggtgt acagttggct ccaaaaggcc gtaccccagg
      901 cttggtttag cttgtcgatg gtctggtatt tattctgcag aaaaattctg aattctgctg
      961 cagcggcctg attgtagtcg tattgcaccg agggttcgtt gtcgagttgc cagccgatca
     1021 ctctggtgtc gctgccgtaa cgtttggcca gttcttcgat ggtcctgagg gccagctcgc
     1081 ggtatttggg actggaaaag gaggcgtgtt gccgggcacc gtgatcggag tgatttccat
     1141 tttcgtgctt gatcagaatc tcagggtatt ttctgctcat ccagaccgga ggagtggctg
     1201 tggaggtgca taaaatcacc ttgagttggt atttatcggc cagatcgatg gctctgtcca
     1261 gccattcaaa gtgataacgg ccttcttcgg gttccagttg agcccaggca aattcgccca
     1321 tatggacggc ttcaaacccc agcccggcca tgttcttgaa atctctttcc cattgatttt
     1381 cgggccagtg ttcgggataa taatacaccc aggtttgact aagttcgctc gctttgaaga
     1441 aagggttttg gccattgctt tctaaaacaa agaagaagag cagtaaaagc aaaaacctta
     1501 atttaaagtt taaagcttga aattcaatct taaaagcgta tatttgtctc ttcatttccg
     1561 gtattaaaaa taagggtcac aaagtaaaca aaaataagca cctgaacgaa aacttgtttg
     1621 caaataatga cataaattct tataaaattg acatttaggc gcttaactag aattttaata
     1681 cgatggcatt gagcgaagat aatcatttta attacttgac ggttaatgaa acagatcaaa
     1741 agtgggggct ttatgtgacc acttgcggat tccaaagcat taaaccgcat tccagtatta
     1801 atccttattt gaacaaacac ctcagctcct acgctttcaa tacaaaacaa ggccgggtgt
     1861 tggatgaatt tcagctcttg ttcgtagtga aaggcagtgg tttttttcaa tcggcctccg
     1921 ttccaaaaac caatatagag acaggaagca tgattatgct ttttccccag gagtggcata
     1981 attacagttt cagtaaagaa acaggctggg atgaattttg ggtgggtttt agcggagagc
     2041 atgcccggtc tattgtgcat caaggttttt tccggaaaga agaacccatt ttcaaattag
     2101 gcatcaacga tgagtttgtt tctctttacc ggcagctcat cagttatgcc gaacaggaaa
     2161 atcaggccta tcagcaaatt atttccggaa ttgtcaggca tatgttgggt ttgctgtatt
     2221 acaaatcgcg taatgtcgtc gtcagtaata aatcggtagt agacaaaatc aaccgggcca
     2281 ggataatgat gcgtgaaaat gtgtcttcgc cccaggctcc cgaagaaatc gcctcgcaac
     2341 tgggggttag ttattcctgg ttcagaaaag cctttaaaaa atacacaggt atttcgccgg
     2401 ctcagtttca gatgaacctg cgtttgcaac atgccaaaga tttgctgatg aacagcgata
     2461 aatccatcac cgaaattgct tacgaactca attttgagag tacagggcaa ttttctacct
     2521 ttttcaagca taaaaccggc accacgccca gggaattccg ttcggttgct tctgccccgg
     2581 ggccttattg agcctgttac cgttgatact agtgttgttg ataacaagga atccgtttct
     2641 ttggccccat taagcctgta atagttaata ccgggttttt attgaaacct gtttttccag
     2701 agccttttca gaaattcttt cagcctgttt tccgcggcat tctgctgagg ttcccatatg
     2761 cttttgccct tgatttcggg aggcaggtag tcttgttcga caaagttgtc acggtaatcg
     2821 tgggcgtatt tgtagtcttt gccgtaatcc aggtctcgca taagttgggt aggagcgttt
     2881 ctcagatgca gcggcacact gacattgcct gtttgacgca ccatagacga agctttttct
     2941 atggccaggt aggctgaatt gcttttgggg ctggatgcta gataaatagc ggtttccgaa
     3001 aggataatcc gactttcggg ccagccgatt ttatggatgg catcgaaaca ggcgttggcc
     3061 agtaacaagg cgttgggatt ggccagacca atgtcttcgg aggcggaaat cagcatgcgg
     3121 cgggcaatga atttggggtc ttcgcctccc tcgaccattc tggccagcca gtaaacggct
     3181 gcatccggat cgctgcctcg tatggacttt ataaatgcag agacgatgtc gtaatgcatt
     3241 tctccgtctt tgtcgtaagc caaggggttg gcctggacgc ttgcaagaac attttcgtcg
     3301 ttgatgatta cagggtcttc gggctggtat tgggtgagca gttcaattat gttgaacagc
     3361 ttgcgggcat ccccacctgc aagacgaaac agttggccac tctcctcgac caaaatccgg
     3421 cgttttttta gttccgggtc tttgctgagg gctctgtcga gtaattcttg caaatcctgt
     3481 tcctcgagag gctttagcac gtagacctgg cagcgcgaca gcaggggagt tacaacttcg
     3541 aacgagggat tttcggtggt agctccaatc agggtaacaa tacccttttc gacggcagcc
     3601 agcagggaat cttgctgaga tttgctgaag cggtgtattt cgtcgatgaa aagtatggga
     3661 ggtttttgtt gaaacaagcg gttgtttttg gctgtttcga tgagttcacg tacgtccttc
     3721 actcctgcgt tgacggcgct caggctgtaa aaagggcact ccagtttata agctataatg
     3781 ctggccagag tggtcttgcc cacaccggga ggtccccaga gaataaaaga cgacaaagtg
     3841 cccgaatcga tcattttccg taaaatggcc ccttcgccaa ccaggtgttt ttggccggca
     3901 tagtcatcca aagtcagagg acgcattctt tctgccagag gtctagccat ttgcgtaagt
     3961 tacttgtcgg tgataaaacg ggaatcgcaa aaatacaaac taaataaata aaactaaact
     4021 cttgtcgtaa gaaaataaat cactaatttc gcggcgtttt tctaagcttc gattcatttt
     4081 atatattttc ctatgaaaaa gcataatttc tatgcaggac catctatcct gagtgagtac
     4141 actattaggc agaccgcaga tgccattctt aatttcgaag gcacaggcct ctcgctaatg
     4201 gaaatttcac accgcagcaa acagttcgtg gctgtttgcg accaggcacg tgccttgatt
     4261 aaggaattac tccatgttcc cgaagacttt gaagtcgttt ttctgggtgg aggtgccagt
     4321 ctgcagtttg ctatggttcc ttttaatttt ttggaaacca aagcagccta tctcgattcc
     4381 ggatattggg catccaaggc catcaaagag gcaaaattgt ttggagaagt gcagatagtg
     4441 ggttcttcca aagacgacaa ctacagccat ttgcccaaga attatcaggt tccctcggat
     4501 gcggattact tctatttttg cagcaacaat actatctacg gtaccgaact gcacgaagat
     4561 ccccatcccg gcgttccggt aattgccgat atgtcttcgg atattttttc acgccccatc
     4621 gatttcagct cttacggact gatttttgcc ggagcacaga aaaacctggc tccctcgggc
     4681 gtcactttgg taattgtacg caaaagcatg ctcgataaag tgagtcgtcc cattccgacc
     4741 atcctcaact ataaaaccca tgtcgaaaat gaatccatgt tcaatactcc tccggtggtg
     4801 cccatttacg cagccttgca aaccttgctc cggtacaaag aactcggcgg catcgaagtc
     4861 atacatgata tgaataaaca aaaggcagcc ctgttgtacg aagaaatcga caccaatccc
     4921 ttgttcaaag gtactgttac ctgcaaagaa gaccgttccc ttatgaatgt atgctttgta
     4981 atgtccgaag gttacgaaca tctcgaggat gaattcgaca aatacgcctc cgaagccggc
     5041 atggtgggca tcaagggcca ccgttcggtt ggcggtttcc gtgcttccat ttacaatgcc
     5101 atgcctatag ctagtgtaca ggcgctggta gacactatga gaagttttgc acaattaaaa
     5161 acacaaaaat aattatgaag atattagttg ctactgaaaa accttttgct cctgttgccg
     5221 tcgagggcat acgtgaaata atacagggag ccggtttcgg ttttgaactg ttggaaaaat
     5281 acaccgacaa aaacagcctc atcgaagttg tatcccaagc tgatggtgta attatacgca
     5341 gcgatatcat cgaccgtcag gttattgaag cagccggaaa attaaagatt gtggtccgtg
     5401 ccggagcagg ttacgacaac gtagatttgg aggcagccag cgaaaaaggt atttgcgtga
     5461 tgaatacccc cggccaaaat gccaatgccg tagccgaatt ggctatgggt atgatggtat
     5521 acgccgtgcg caatatgttc aacggtactt ccggaaccga attgaaaggc aaagcacttg
     5581 gcatacatgc ctacggaaac gtggggcgta acgtggcccg cattgctaag ggtttcggta
     5641 tggaaatcta tgcgtacgac ccttattgca agccggaagt aatcgaaaga gacggggtga
     5701 aggtcttgac cagcgtagag gatttataca aaaaatgtca gattgtttct ttgcatattc
     5761 ctgctacgga agaaacttcc aaatccatta acgccgatct tctgggacgc atgcccaaaa
     5821 atgccgtctt ggtgaatacg gcccgcaaag aggtgataga cgaaacttct ttgatgatgc
     5881 tgatggagaa taggattgac tttaagtaca tcaccgatat tatgcccgca gccgatgcta
     5941 aatttgccga aaaatttgcc ggccgttatt actccactcc caagaaaatg ggagcacaga
     6001 cagccgaagc caatatcaat gcaggcatag cagccgccaa acaaattgta gatttcttta
     6061 aagaaggtaa tcagacattc agagtcaatc cctaaagcaa catggtaaaa ataagagctt
     6121 tcaaaggctg gagaccgcca cggaatttgg tagaaaaagt ggcatcccgg ccttacgacg
     6181 ttttaaattc cgaggaagcc aggcaggaag ctgccaacaa cgagtactcc ctgtatcgta
     6241 tcatcaaacc cgagattgat tttcctccgg gaacagatga acacgaaccc aaggtgtaca
     6301 ccaaggctaa agaaaatttt gatctttttc gtaaaaaagg ctggttggtt caagacaaca
     6361 aagctatgta ttacatctac gcccagacca tgaacaacag gacccaatac ggactggtgg
     6421 tttgtgccag cgtggaagat tacatgaacg gccttatcaa aaagcatgaa ttgacccggc
     6481 gcgacaaaga agaagatcgt atgaagcacg tcagggtgaa caacgccaat attgaaccgg
     6541 tgtttttggc ttacccggct caggaggctc tggataagct ggttgcaggg catattcgta
     6601 aagagcctgt ttaccggttt actgctccgg atggctttgg ccatcaattc tggcttatcg
     6661 acgacgacga aagcatcggc actatcaccc gctgtttcga acagataccc gctctgtaca
     6721 ttgccgacgg acatcaccgc acagccgccg ccgctttggt gggcaacgaa aagaaagaac
     6781 aaaatcctca tcacaacgga caagaggaat acaatttctt tctggccgtt tgttttcccg
     6841 ataatcaatt gcagatcatc gattacaaca gggtagtcaa agacttgaac gggcacgata
     6901 aacaatcgtt tctcgccgct ctcgaagaag attttatcgt cgaaagcaaa ggctcgcaga
     6961 tttataaacc ttcccgtttg cacaacttcg gtctctatat cgacagggaa tggtattcgc
     7021 tcacagccaa accgggaaga tacgacgata aggatcctct gggcgtattg gatgtgaaca
     7081 tttcatcgcg tctgatattg gatcgtattt tgggcatcaa agatttgcgc agcgataaac
     7141 gcatcgattt tgtcggcggt attcgtggcc ttaaagagtt ggagcgccgc gtggactcgg
     7201 gcgaaatggc cctggctctt gctctatacc ccgtttcgat gaagcaactc atcgatattg
     7261 ccgacagcgg caatataatg cctcccaaaa ccacctggtt tgaacctaaa ttacgttcag
     7321 gattggtaat acatacctta gacgactgaa aggtgctgaa cgcctgcatt gtaccatgat
     7381 cgatacatac a
//
LOCUS       DAOSTD010000082         7303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_9510_length_7303_cov_15.351683, whole genome shotgun sequence.
ACCESSION   DAOSTD010000082 DAOSTD010000000
VERSION     DAOSTD010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9510_length_7303_cov_15.351683"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1484
                     /locus_tag="PLI37_06170"
     CDS             156..1484
                     /locus_tag="PLI37_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOQ58629.1"
                     /translation="MHRKRSLKYLVFNLFFLFFILSLPADAAFTASGGSNGRPYEYIP
                     AASTGLERVYIFDGLQQAELRFNTADSSNWTWYRYQTGLEDAQAVPLTDISLESDATV
                     LKNPGAYGYYVSQGDQIKAVYIIDYSAHKLDYTKLEGVDNAEDICSSLQLVVEGIQED
                     LSYYALSGSPARRTVDRIHSLTWTDLEWDEEKEQYLPVDKNSNKEGFLSGFIVDAPLQ
                     NTRFKLEGDQFAAHFGLSTKSIETSYQAVRVSTHAKAVPEEREQADNEIGAGSAGSLT
                     GSAPFKLDFYSNVNPATRYTEWYIYTNPGSKEEYLRRTETDINYTFNQAGSFEVKLLA
                     SNNICKDSATFTVRVTESSLDCPNFFSPRSTPGDNDEFKVAYKSLIKFHGRILNRWGN
                     VLFEWTDPSLGWDGTFKGKPVSPGVYFYIISATGSDGIEYRKKGDINLLE"
     gene            1481..2422
                     /locus_tag="PLI37_06175"
     CDS             1481..2422
                     /locus_tag="PLI37_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOQ58630.1"
                     /translation="MMKLNKYIIAVVFLMYNGFFSMAQSIYPQALIHPIDLPLRTEFC
                     GERIDLRRWDLRERFDRELLAMNYMHSSSIQLIKKANRIFPLIEPILKANQVPDDFKY
                     LACIESNLNSGALSPAGAAGIWQFMPETAREYGLQVDDQVDERYHLEKSTVAACKYLK
                     DAYRKFGNWSTAAAAYNGGINRIANELEKQGVDNALDLYLNEETSRYVFRIMAAKDFM
                     ENPAKYGFIFSAEYLYMPLQYNYVSIHKSVGDWVEFAKEHGLTYLQFKEANPWLRGSR
                     LNNPRGKIYNIAIPMESSQYFDVKNLYIHQKNWIRNK"
     gene            2440..5262
                     /gene="uvrA"
                     /locus_tag="PLI37_06180"
     CDS             2440..5262
                     /gene="uvrA"
                     /locus_tag="PLI37_06180"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOQ58631.1"
                     /translation="MDDVSKDHICVYGAREHNLKNIDVQIPRNSLTVITGLSGSGKSS
                     LAFDTIFAEGQRRYIETFSAYARNFLGNRERPDVDKITGLSPVISIEQKTTNKNPRST
                     VGTQTEVYDYLRLLYARAGEAYSYLSGEKMVKYTEEQVLELILEKYNNKAIYVLAPVV
                     RSRKGHYKELFETLRKKGYLNVRVDGELQEIIPGMRLDRYKNHDVEVLVDKLIVSPND
                     RDRLKKSLQVAFKQGKGLIMIYDKDQDELRHYSSQLMCPVTGLSYGDPAPHSFSFNSP
                     QGACPRCKGLGIINQIDFNKIIPDNNKSIYEGGIVPLGKYRNSMLFWQIEALGEKHGF
                     KLKTPIRDIAPDAMDEILNGCEERLPIKNTTLGLYNYNVHYEGVVKYIQLQQDDDSPS
                     DAQKWAEQFISTAVCPECQGLRLKKEALHYRLAGKNIAELAAMDIKELALWIKDLDRH
                     LNDKQKRIASEILKEISQRLQFLLDVGLDYLSLNRPSAGLSGGESQRIRLATQIGSQL
                     VNVLYILDEPSIGLHHRDNRRLIDSLKTLRDKGNSIIVVEHDKEMMLASDYILDLGPR
                     AGRLGGHLSFAGSPGDLLQASTLTSDYLNGKLRIKAPTAYRPGNGRVLSLKNCRGNNL
                     KQVNVDFPLGKLIVVTGVSGSGKSSLINHTLRAALSKHFYRSHQEPLPYDSIEGLEFI
                     DKVVDVDQSPLGRNPRSNPSTYCGVFSDIRNLFVNLPESKIRAYKPGRFSFNVAGGRC
                     ETCKGNGYRAIEMNFLPEVFVPCESCRGKRYNRETLEIRFKGKSIADVLDMTINMAVD
                     FFENQPQILHKIKTIQEVGLGYIKLGQPSNTLSGGESQRVKLAAELSKRDTGNTLYIL
                     DEPTTGLHFDDIRVLMQVVNRLVDKGNTVIIIEHNPDVIKMADHIIDMGPEGGKEGGR
                     ILFSGTPEELALSKQSYTAACLKDLL"
     gene            5259..7172
                     /locus_tag="PLI37_06185"
     CDS             5259..7172
                     /locus_tag="PLI37_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOQ58632.1"
                     /translation="MSKLQPFVQVENLSKSFGDLVLFHKISFGIAQQQRVALIAQNGK
                     GKSTLLDILAGNKDYEEGSVVFRRGLKVAYLKQTPEFPVGLTVFQACFQAANEVTETI
                     ARYELKMQEIAGKSIDAEAEELLHLMDHYKAWDYEVRIKQILSKLKIPNFTQKVGQLS
                     GGELKRLALANVLITEPDLLILDEPTNHLDLDMIEWLENYLTKSGITLLMVTHDRYFL
                     DRVCDTILELEDKQLYCYNGNYAYYLEKRRERLEISNTEWERAQNLYRKELDWMRRMP
                     QARGTKAKARSDAFYELEERVSQRHKTEQLRLGVKASYLGSKVLEAKYISKSYGELKL
                     LEDFYYQFAPFEKVGIVGANGSGKTTFLRLLMGEEPCDRGSIDIGSTVRFGYFSQEGL
                     SFDENKKVIDVVRDIAEEISLGNGKKLSASQFLTHFLFSPEVQHHYASKLSGGEKRRL
                     YLCTVLMTNPNFLVLDEPTNDLDILSLNILEEYLVSFKGCVLVVSHDRFFMDKIVDHL
                     LVFEGDAIVRDFPGNYTQYREWKEQQEALLRKEKESERKSKSNLPDIEPKKEESSAHR
                     KRTYREEQEFIALEKEIAQIEENIALIENDLASGQLEGGAIEQKCIELSRLNQELDKK
                     ALRWMELGELEKK"
BASE COUNT         2101 a         1650 c         1753 g         1799 t
ORIGIN      
        1 tgttttgcaa atcagagtat aataataccg cacagacagt aaattcaact agttgcgtag
       61 ggatgctgaa tctgcgagtc ctacatgaaa acagggtaat tttatttttt gattatttat
      121 atctatcttt gtgcgctttt tttagaaact ctgatatgca tcgaaagaga tcacttaaat
      181 atctggtttt taatttattc tttttattct ttatacttag tttgcctgcc gatgctgctt
      241 tcacggccag cgggggatcg aacggccggc cttacgaata cattcctgca gcaagcacag
      301 gcctggagag ggtctatatc ttcgatggac ttcaacaagc agaattgcgc ttcaacacgg
      361 cagattcgtc aaattggacc tggtatcgtt accaaaccgg ccttgaggat gcccaggcag
      421 tcccccttac cgatatcagt cttgaaagtg acgccactgt gttaaagaat cccggtgctt
      481 acggatatta tgtgtcgcag ggagatcaga taaaggccgt ctatatcatc gactattcag
      541 ctcataagct ggattatact aagcttgaag gcgttgacaa tgccgaagat atttgcagca
      601 gcctgcagct cgtggtcgaa ggtattcagg aggatttgag ctattatgcc ctgagcggaa
      661 gcccggcgcg ccgtaccgta gatagaattc acagcctgac ttggactgac ttggaatggg
      721 acgaagagaa ggagcaatat ctgcctgtcg ataaaaacag caataaagaa ggctttttgt
      781 ccggtttcat cgtggatgct cctctgcaga ataccagatt caaactcgaa ggcgaccaat
      841 ttgccgccca tttcggcttg agtacaaaaa gcatcgaaac aagctatcag gctgttcgcg
      901 taagtactca tgccaaagcg gttcctgagg aaagggagca ggccgacaac gaaataggag
      961 caggttcagc cggttcgctt acgggatcgg ctccctttaa actggatttt tacagtaatg
     1021 tgaatccggc aacccgttat accgaatggt atatttacac caatcccggc tcaaaggagg
     1081 aatacctgcg ccgcacagag accgatatca attacacttt taaccaggcc ggcagctttg
     1141 aagtgaagtt attggccagt aacaatattt gtaaagattc agccaccttc accgtgcggg
     1201 taacggaatc ttcgctggat tgccctaatt ttttcagccc tcgttccact ccaggagaca
     1261 acgacgaatt taaagtggcc tacaaatcgc tgattaaatt tcatggacgc atcctgaatc
     1321 gttggggcaa tgtgttgttc gaatggacag atccttccct tggttgggat ggaactttca
     1381 aaggtaaacc ggtatctccc ggcgtgtatt tttacatcat cagtgctacc ggctccgatg
     1441 gtatagaata ccggaaaaaa ggagacataa atttattaga atgatgaaat tgaacaagta
     1501 tataattgct gttgtttttt taatgtacaa cggtttcttt tcgatggctc agagcattta
     1561 tcctcaggct ttgatacatc cgatagatct tcctctccgg acagaattct gtggtgaacg
     1621 catagacctg aggcgttggg atttgaggga acgcttcgac agagagcttt tggcgatgaa
     1681 ttacatgcac tcgtcttcca tccagctcat aaaaaaagcc aaccggattt ttcccctcat
     1741 agagcccata cttaaggcca accaagtccc cgacgatttt aaatacctgg cttgcatcga
     1801 aagcaatctg aattccggag ctttgtctcc cgcgggagcg gccggaatat ggcaatttat
     1861 gcccgaaacg gctcgcgaat acggcctgca ggttgacgat caggtggacg aacgttacca
     1921 cttggaaaaa tctaccgtag cggcctgtaa ataccttaag gatgcctacc gtaaattcgg
     1981 caactggagt acggcggcag cagcctataa cggcggcata aatcgcatag ccaacgaact
     2041 ggaaaagcag ggtgtggaca acgcgctgga tttatatttg aacgaggaga cttcgcgtta
     2101 cgtgttccgt ataatggccg ccaaggattt catggaaaac cccgccaaat acggttttat
     2161 cttttcagct gaatatttgt atatgccttt gcaatacaac tacgttagta ttcataaatc
     2221 tgtcggtgac tgggtggaat ttgccaaaga acacggctta acctacctgc aatttaagga
     2281 ggccaacccc tggctgcgcg gtagccggct gaacaatccg cggggtaaaa tttacaacat
     2341 cgccattccc atggagtctt cccagtattt tgatgtcaag aatctctaca tccatcaaaa
     2401 aaactggatc agaaacaagt aatcgctttg taaaacccaa tggacgacgt atcaaaagac
     2461 catatttgtg tatacggggc gcgggaacat aatctgaaga acatcgatgt tcaaattccc
     2521 cgcaatagtc tcacggtgat taccggctta agtggcagcg gtaaatcctc tttggctttt
     2581 gatactattt ttgccgaagg gcaacgtcgt tatattgaaa ctttctcggc ctacgcccgc
     2641 aattttttgg gcaatcgaga acgccccgac gtggataaga taacgggatt gagtccggtg
     2701 atttccatag aacaaaaaac cacgaacaag aatccccgct ctacggttgg cacccagacc
     2761 gaagtgtacg attatctccg tctgctttac gctagggcgg gagaggccta ttcttatctg
     2821 agcggagaga agatggttaa gtataccgag gaacaagtgt tggagctgat tttggaaaag
     2881 tacaacaaca aagccattta tgtgttggcc cctgtagtgc gttcccgcaa aggccattac
     2941 aaggaattgt tcgaaacgct caggaaaaaa ggctatctaa atgttcgtgt tgacggagaa
     3001 ttacaggaaa tcattccggg catgaggctg gaccgctaca agaaccacga tgtggaagtg
     3061 cttgtcgata aattgatcgt cagtcctaac gacagagacc ggcttaaaaa aagcttgcag
     3121 gtagcattca aacaaggcaa aggcctgata atgatatacg acaaggatca agacgaactc
     3181 agacattaca gtagtcaact gatgtgtccg gttacagggc tttcctacgg cgatcccgcc
     3241 cctcattctt tttcgttcaa ttctcctcag ggagcttgtc cgcgatgcaa gggactgggc
     3301 ataatcaacc agattgattt caataaaatt attcccgaca ataacaaatc catttatgag
     3361 gggggaatag tgcctctggg taaatacaga aacagtatgt tgttttggca aatagaggct
     3421 ttgggcgaaa aacacggttt taaactcaaa acacccatca gggatatcgc tcccgacgcc
     3481 atggatgaga ttttaaacgg ctgcgaagaa cggctgccta tcaaaaacac tacgctgggt
     3541 ctgtataatt acaatgtgca ctacgaaggt gtcgtcaaat acattcaact gcagcaagac
     3601 gacgacagtc cgtcggatgc gcaaaaatgg gccgaacaat tcatcagtac tgccgtatgt
     3661 cccgaatgtc agggtttgcg gctcaaaaaa gaagccctgc attatcgcct ggccggtaaa
     3721 aacattgcag aactggccgc gatggacatc aaagaactgg ctctgtggat aaaagatctg
     3781 gatcggcatt tgaacgataa acaaaaacgc atagcctccg aaatactaaa ggaaatttcg
     3841 caacgtctgc aatttttgtt ggacgttggc ctggattatc tttcgcttaa caggccttcg
     3901 gcaggactat cgggcggcga aagccaacgt atccgcctgg ccacgcaaat cggttcccag
     3961 ctggtaaatg tgctgtatat tctggatgaa cccagcatag gcctgcacca cagagacaac
     4021 cgacgtctga tagactccct gaaaaccctc agggacaaag gcaattcgat tattgtggta
     4081 gagcacgata aggaaatgat gctggcttcc gattacatac tggacctggg gccgagagca
     4141 ggcagactgg gaggacatct gagttttgca ggcagtcccg gggacttgct gcaggcttct
     4201 accttgactt ccgattacct gaacgggaaa ctacggataa aggctcccac agcgtatcgc
     4261 ccgggaaatg gccgtgtctt gagcctgaag aactgccgag gtaacaacct caagcaagtg
     4321 aacgttgatt ttcccctggg aaaattaatt gtagttacag gggtttccgg cagtggaaaa
     4381 tccagcctga tcaatcacac cctgcgtgct gccctgagca aacattttta tcgcagtcat
     4441 caggaacccc tgccttacga tagcatcgaa ggcctggaat tcatcgataa agtggtggat
     4501 gtggatcagt ctcccctggg acgcaatccg cgttccaatc cttctaccta ttgcggagtg
     4561 ttttccgaca ttcgcaatct atttgtcaat ctgccggaat caaaaatccg agcctacaaa
     4621 ccggggcgct tttcttttaa tgtagccgga ggacgttgtg aaacttgtaa ggggaacggt
     4681 tacagggcca tagaaatgaa ttttttgccc gaagtttttg tgccctgtga gtcttgccgg
     4741 ggcaaacgct acaaccgtga aactctggaa attcgcttta agggtaaatc cattgccgat
     4801 gtgctggaca tgaccatcaa tatggcagtc gatttctttg agaatcagcc gcagatattg
     4861 cataaaatta aaaccattca agaggtgggc ctgggctata ttaaactggg acaaccttcc
     4921 aacactttgt caggcggaga atctcaacgt gtcaagctgg ccgcggagct ttccaaaagg
     4981 gatacgggca acaccctgta cattttggac gagcccacta ccggtctgca tttcgatgac
     5041 atcagggtat tgatgcaggt ggtcaaccgc ttagtggata aaggcaatac ggtcattatc
     5101 attgaacaca acccggacgt catcaaaatg gccgaccaca tcatcgatat ggggcctgaa
     5161 ggaggcaaag aaggcggcag gatactcttc agcggtacgc cggaagagtt ggctttgagc
     5221 aagcaaagct atacggcagc ctgtcttaag gatttattat gagcaaatta cagccttttg
     5281 ttcaagttga gaatctgagc aaatcttttg gcgatttggt cctttttcac aaaatctcct
     5341 ttggcattgc acagcaacaa cgggttgcac tgattgccca gaatgggaag ggcaaatcta
     5401 ccttgctcga tattttggcc gggaacaaag attacgagga aggttccgtg gtttttcgac
     5461 gtggtctgaa agtagcctat ttaaaacaaa cacccgaatt tccggtcggt ttgaccgttt
     5521 ttcaagcctg ttttcaagcc gccaacgagg tgaccgaaac catagcccgt tacgagctca
     5581 aaatgcagga aatagccggt aaatccatag atgccgaagc cgaagaactg ttgcatctga
     5641 tggatcacta caaagcctgg gattacgaag tcagaataaa acaaatccta agcaaactga
     5701 agattccgaa ttttacccaa aaagtcggac aactgtcggg aggagagctt aagcgtctgg
     5761 ctttggccaa cgtgctgatt accgaacccg atttgcttat cctcgacgaa cccaccaacc
     5821 acctggacct ggatatgatc gagtggctgg aaaattacct gaccaaatcg gggataactc
     5881 ttttgatggt tactcacgac cggtattttc tggaccgtgt ctgcgacacg atactggagc
     5941 tcgaagacaa acaactttat tgctacaacg gcaattacgc ctattacctg gaaaagcgac
     6001 gcgaaagact cgagatttcc aataccgaat gggaaagggc gcaaaatctg tatcgcaaag
     6061 aactcgactg gatgagacgt atgcctcagg ccagaggaac caaagccaaa gctcgcagcg
     6121 acgcttttta tgaactggaa gaacgggtat cgcaacggca caaaacagaa cagttgcgtt
     6181 tgggagtaaa agccagttac ctgggctcca aagtgctcga agccaaatac atctcaaaat
     6241 cctacggtga gctgaaactt ttagaagact tttattatca gtttgctcct ttcgaaaaag
     6301 tcggtatagt aggcgccaac ggcagcggta aaaccacctt tctccgcctg ctgatgggag
     6361 aggagccttg cgaccggggc agtatagata tcggcagtac cgtacgtttc ggctatttta
     6421 gccaggaagg cttgtcgttt gacgaaaata aaaaagtgat cgatgtggta cgcgatatcg
     6481 ccgaagaaat ttccctggga aacggtaaga aactgagtgc ttcgcaattt ctcactcatt
     6541 ttttattcag tcccgaagtc cagcaccatt atgcctccaa gttaagtggc ggcgaaaagc
     6601 gccggctgta tttgtgcacc gtattgatga ctaatcccaa ttttttggtg ttggacgaac
     6661 ccaccaacga tctggatatt ttgagcctga acatcctcga agaatacctc gtttcgttca
     6721 aaggttgtgt attggtggtt tcgcacgacc gtttttttat ggacaaaata gtggaccatt
     6781 tgctggtctt cgaaggcgat gccatcgtcc gggattttcc gggcaactac acccaatacc
     6841 gagagtggaa agaacagcag gaagctttac tcagaaaaga aaaagaatcg gagcgaaaaa
     6901 gcaaaagtaa cctcccggat attgaaccga aaaaagagga aagttccgcc caccgaaaac
     6961 gaacttaccg cgaagaacag gaatttattg ctctcgaaaa ggaaattgca caaatcgaag
     7021 aaaacattgc tctaatagaa aacgatctgg cttcggggca attagaaggt ggagcgattg
     7081 aacaaaaatg cattgagctt tccaggctga atcaagaact cgacaaaaaa gccctgcgct
     7141 ggatggaact gggcgaattg gaaaaaaaat aagaaaaagc ctttaaagct tttgccgttt
     7201 agcataaata acgtactttt gcgcccgttc aaaaaacaga acaagacttc gtagctcagt
     7261 tggtagagca attgactctt aatcaatggg tcgagggttc gag
//
LOCUS       DAOSTD010000083         7275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_9557_length_7275_cov_15.011219, whole genome shotgun sequence.
ACCESSION   DAOSTD010000083 DAOSTD010000000
VERSION     DAOSTD010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9557_length_7275_cov_15.011219"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1779)
                     /locus_tag="PLI37_06190"
     CDS             complement(178..1779)
                     /locus_tag="PLI37_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HOQ58633.1"
                     /translation="MKISHKLIFLCLLITCCVLSSCQSEKQSDLRARMQEWDDILWEK
                     SDIEIDEGPQAILDSLESINFKSLNYKNRAYYYLLQTIARDKSYYVFENDSVISIAVK
                     WYKNKPDAYNYSRSLFYHAIVLSQIHTNDSLMFFSIKTAENVFWEKKLNDSLLYGRIC
                     VFMGSLHYSRGNYDISNDYYEKAASTFKAIGNTDRYISAKVNQVWYLISIGEHDAALN
                     MLNNIDYPEKISDKALSYIYNVYSGYYTAIKNYSAAIEYNKLDFALNNVPKTPEDSAT
                     HYFSLIKNYCRAGIPDSAIRYAKQLELSLRENNPNNHFYFIGMSDAYDQAEMGDIGNE
                     YLRKAYFSLRSNIYDNSQQRILELEKKYDMSRKEYELMKARQEKKLFVAFFAITVLVF
                     IITIIVTYQHRQIQKQKNLLIQEEKEHIAKINVLQKKAINLFPSFVQISNTVLQRNYS
                     LELQEDMDKAIFSLKVALINELSSIFPVEKDSIDYENLSEKEQLIFLLTEFGFSNAEI
                     ATILLITEANVRSTKSKIKKKSETF"
     gene            complement(1800..2861)
                     /gene="thiL"
                     /locus_tag="PLI37_06195"
     CDS             complement(1800..2861)
                     /gene="thiL"
                     /locus_tag="PLI37_06195"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOQ58634.1"
                     /translation="MDGLDDITRPVERERTEIAKLGEFGLIEHLTAGNTLNQATTVLG
                     PGDDAAVLHYGEDKDILLSTDLLLEGIHFDLSYVPLKHLGYKAAVVNFSDICAMNGTP
                     SQLTVSVGISKRFSVEDMEELYAGIKIACKGYGVDLIGGDTSASLTGLSLCLTCTGEV
                     AKGQAVLRSGAKVNDLICVSGNLGAAYMGLQLLEREKLVYEGQQDFQPDFAGKEYLLQ
                     RQLRPEARKDIVEKLAQKKIRPTAMIDVSDGLSSDLLHICKQSNCGCRIFEDKLPIDY
                     QTASLAEEMNMNLSTAALHGGEDYELLFTVPLELHETIENWPDVRIIGYICHEQDGRK
                     LITRDNSEFDIIAQGWKMA"
     gene            complement(2864..3676)
                     /locus_tag="PLI37_06200"
     CDS             complement(2864..3676)
                     /locus_tag="PLI37_06200"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOQ58635.1"
                     /translation="MYETIQKTLQWLEANTKLRPSIGIILGSGLGNLVDQIASPQRIP
                     YADVPNFPVSTVEGHSGNLVLGMLGGKAVMAMQGRFHYYEGYSMKELSFPLRVMKAIG
                     IKTLFLSNAAGGLNPTFKVGDIMVITDHLNLFPEHPLRGKNDPRLGTRFPDMGQPYSK
                     ILIERATVVAKKLNIPLKQGVYAGWQGPSFETPAEYRMLHLLGADACGMSTVPEVIVA
                     RHAGMDVFGLSIITNSASSASTEKASHEEVQEAAIKAQPLMTTLITELIKTL"
     gene            complement(3639..>3994)
                     /locus_tag="PLI37_06205"
     CDS             complement(3639..>3994)
                     /locus_tag="PLI37_06205"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOQ58636.1"
                     /translation="CKLMCFCGIASPNSFIGWVRQSFPEAPALIFPDHHEYKASDLEK
                     IYSAFERLENGNKCIITTQKDYMHLKNNPLFEALTPYIYYIEIDIHFVDGQEDVFKKL
                     ITDYVRNHTKNAAMA"
     assembly_gap    3995..4094
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4095..4897)
                     /gene="lpxK"
                     /locus_tag="PLI37_06210"
     CDS             complement(<4095..4897)
                     /gene="lpxK"
                     /locus_tag="PLI37_06210"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOQ58637.1"
                     /translation="MPPKRPARPLLLPLSPIFGGIVRFRNLLYDRSICKTSAFEIPTI
                     CVGNISVGGTGKTPHTEYLTKLLQKQYKLAVLSRGYKRKTKGFVLASNDSKVEDIGDE
                     ALQMAGKFPGITVAVHENRVKGMQLLLKARPEIQVVLLDDAFQHRRLKAGLSIVLTDY
                     GRPVYDDFLLPAGNLRETPRALSRADIVICSKCPPDISAEKRKQIASRLALKKGQNLF
                     FSGIRYGKLRAGFPGKGAYSAQSSLAELKQKDCKLMCFCGIASPNSFIG"
     gene            complement(4906..6672)
                     /gene="sppA"
                     /locus_tag="PLI37_06215"
     CDS             complement(4906..6672)
                     /gene="sppA"
                     /locus_tag="PLI37_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOQ58638.1"
                     /translation="MKQFFKFSLASALGVVIALVVLSLIWLLVLLGLASSTESTPKLQ
                     NNTLLKIELKGVLSDYAQEDPFSEVANELFGMDQAAKIGLDEILAAIKKAKDNENISG
                     IYIESSSLQCGIASAQEIRKALSEFKESGKFIYAYANELFNQKEYYICSVADSLFLNP
                     YGMLHFAGMSATPVFLTEMLDKIGIEPEIFKVGTYKSAVEPLINKEMSKENREQTLSY
                     LSGIWNNMLQEISADRNISVDKLNQLADRFMSLQPTEETVESGLIDDLFYPLEMMQFF
                     ADKLDSVKVKDLKMISVRGLNKIPEKQTEFIKEKVAVLYAQGGISTEAPDGISSKKLV
                     REIDKLRENESVKAVVLRVNSPGGSAFASEQIWYALGELKKEKPLVVSMGDYAASGGY
                     YISCNADKIIAAPTTLTGSIGIFGLFFNARELTEKIGLDFDMVKTNQHADFGDFSRKM
                     TPAEKILMQNSVERGYDLFVERCAQGRGMENHQIRLIGEGRVWTGEQALERKLVDELG
                     YLDQAIDAAVELAELEKYRLVTYPEKKDLMTRVLESMNKNVSTRIIKNYLSEYSPDLL
                     ELMETKLEPGILARMPYNLIIQ"
     gene            7131..>7275
                     /locus_tag="PLI37_06220"
     rRNA            7131..>7275
                     /locus_tag="PLI37_06220"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1871 a         1510 c         1585 g         2209 t
ORIGIN      
        1 aattcccaaa aacaataatg ataatacaag tttgttcata aggcaaaaat ttaaaaaatg
       61 aataattcaa gtaactaaac aagaaaacga tgtgtcaaaa atataacgaa tttcgcttaa
      121 ttgtttgagt tgcccaaaaa gtttcgctgt aacagcttgt ataccaacaa agtaatatca
      181 aaaagtttcg ctcttttttt tgattttcga cttggttgag cgaacatttg cctctgttat
      241 gagtaaaatt gtggcaattt ctgcgttcga aaaaccaaat tcggtgagca aaaatatcag
      301 ttgttctttt tcactgagat tttcataatc tatggaatct ttttctacgg ggaaaatgga
      361 cgataattcg ttaataagcg cgactttcaa agaaaaaatg gctttatcca tatcttcctg
      421 aagttctaaa ctgtaatttc tttgtaaaac agtattgctt atttgaacaa aactggggaa
      481 aagattgatg gcttttttct gtagaacatt tattttggcg atgtgttctt tttcttcctg
      541 aataagtagg ttcttttgtt tttgaatttg tctgtgctga tatgtaacaa tgatagtaat
      601 aatgaaaaca agaaccgtga tggcaaagaa agcaacgaat aatttttttt cttgcctggc
      661 tttcatcagt tcatactcct ttcgcgacat gtcgtatttt ttttccaatt caagaatacg
      721 ttgctggctg ttatcgtaga tattgcttcg taagctgaaa tatgccttgc gcaaatactc
      781 attgcctatg tcgcccattt cggcttgatc gtatgcatcc gacatgccta taaaataaaa
      841 atggttattg ggattgtttt ctcttagcga aagttccaat tgtttcgcat agcgaatggc
      901 actgtcggga atccctgccc tacagtaatt tttaattagt gagaaataat gtgttgcgct
      961 gtcttcggga gttttgggaa cattgttcag agcgaaatcc agcttattat actcaatagc
     1021 tgctgaatag tttttaatag cagtgtaata accactgtag acattgtaaa tgtacgatag
     1081 ggctttgtcg gatatttttt cgggataatc tatattattc agcatgttta gagcagcatc
     1141 gtgttcaccg atggatatga gataccatac ctgatttact ttggcggaaa tatatcggtc
     1201 ggtgttacct atggctttaa aagtggaggc agccttttcg tagtagtcat ttgaaatatc
     1261 gtaatttcct ctggaataat gtaagctgcc cataaaaaca cagattcggc cgtaaagcaa
     1321 agaatcgttc aatttcttct cccaaaacac gttttcggcc gttttgatcg agaaaaacat
     1381 tagagaatcg ttggtatgaa tctgagaaag cacaatggca tggtaaaaca aactccggct
     1441 gtaattgtac gcgtcaggtt tgtttttata ccatttcacc gcaatagaaa taacagaatc
     1501 gttttcaaat acgtaatacg atttgtctct tgcaatggtt tgtaacaaat aataatatgc
     1561 cctgtttttg taattcaggc ttttaaagtt aatgctttca aggctgtcaa ggatagcttg
     1621 agggccttcg tcaatttcga tgtcgctttt ttcccacaag atatcgtccc attcttgcat
     1681 acgggctcgt aaatccgact gcttttcgct ttggcaggaa ctcagcacgc aacaagttat
     1741 aagcaggcaa aggaaaatga gtttgtgcga tattttcata gtattgactt gtacctggtt
     1801 tatgccattt tccatccctg agcgatgata tcaaattctg agttgtcgcg tgtaataagt
     1861 ttacggccat cttgttcgtg acagatataa ccaataatcc ttacatccgg ccagttttct
     1921 attgtttcgt gcaactccag gggcacagta aataaaagct cgtagtcttc gccgccgtgc
     1981 agagctgcag tactcaggtt catattcatt tcttcggcta gggaggctgt ttggtaatct
     2041 atgggtaatt tgtcttcgaa aatgcgacaa ccacagtttg attgtttgca aatatgcagt
     2101 aaatcggacg agagaccgtc cgaaacatct atcatggcgg tagggcggat ttttttctga
     2161 gccagtttct caacgatgtc cttacgggct tccggacgta gttgtcgttg caaaagatat
     2221 tctttaccgg caaaatcggg ttgaaaatct tgttggccct cgtagaccag tttttcgcgt
     2281 tccaacagtt gcagccccat gtaggctgct cccaaattgc cagaaacaca aatcaggtcg
     2341 ttcaccttag ctccgctacg caacacggcc tgacctttag caacctctcc ggtacaggtc
     2401 aggcaaagac tcagtcccgt gagcgatgcc gaggtgtcgc ctccgattaa atcgacgccg
     2461 taacctttgc aggctatttt aataccggca tagagttctt ccatgtcttc gacagagaag
     2521 cgtttggata ttcctacaga aacggtcagt tgagacgggg taccattcat ggcgcaaata
     2581 tcggagaagt ttaccacggc agccttgtac cccaggtgtt tcagaggcac ataactcagg
     2641 tcgaaatgta tgccttcgag taaaaggtcg gtgctgagca atatgtcttt gtcttcgccg
     2701 taatgcagta cggcagcatc gtcgccgggt cccagtacgg ttgtagcttg gtttagtgtg
     2761 tttcctgccg tcaggtgttc aatgagtcca aattctccga gctttgctat ttcggtgcgt
     2821 tccctttcga caggtctggt aatgtcgtcc aatccatcca tgtttacagg gttttaatca
     2881 gttctgttat caatgtggtc atcaggggct gagcttttat tgctgcttcc tgaacctcct
     2941 cgtgagaagc cttttccgta ctagccgacg aagctgaatt ggtaatgatt gaaagcccga
     3001 aaacatccat gccggcgtgt ctggcaacaa tgacttcggg aacggtggac attccgcagg
     3061 catcggctcc aagcaggtgt agcatgcggt actcggccgg ggtttcgaaa cttgggcctt
     3121 gccagccggc gtacactccc tgcttcaagg gtatatttag ttttttggcc acgacagtgg
     3181 ctctttctat caaaattttt gaatagggct gccccatgtc agggaaacgg gtgcccagac
     3241 ggggatcgtt ctttcctctt aaggggtgtt cggggaaaag attcaggtga tcggtaatca
     3301 ccatgatgtc ccctacttta aaagtgggat tcaggcctcc ggcggcattc gatagaaaca
     3361 aggttttaat gccgatagct ttcatcacgc gcaggggaaa actgagttct ttcattgaat
     3421 aaccttcgta ataatgaaaa cgcccctgca tggccataac ggctttccct cccaacatgc
     3481 ccagtaccag attgccgctg tgcccttcta cggtggaaac gggaaaattc ggtacatctg
     3541 cgtagggtat gcgttgggga gacgctattt gatcaaccag gttcccgagc cccgatccca
     3601 gaattattcc tatggaaggt cttaacttcg tgttggcttc aagccattgc agcgtttttt
     3661 gtatggtttc gtacataatc ggttattagt tttttaaaga catcttcctg tccatcgaca
     3721 aaatgaatat ctatttctat gtagtatata taaggtgtca atgcttcaaa gagcggattg
     3781 ttttttaaat gcatatagtc cttttgggta gtaataatgc atttgtttcc gttttcaagg
     3841 cgttcaaaag cactgtaaat tttttcaaga tcggaggctt tgtattcgtg atgatcggga
     3901 aaaatcagag cgggagcctc cggaaaggat tgccttaccc atcctataaa gctgtttggt
     3961 gaagctatac cgcaaaagca catcagttta cagtnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnncatcct ataaagctgt ttggtgaagc tataccgcaa aagcacatca
     4141 gtttacagtc tttttgtttg agttccgcca gactgctttg ggcagagtag gcccctttgc
     4201 cgggaaagcc tgctcggagt tttccatagc ggatacccga aaagaacagg ttctgtccct
     4261 ttttaagtgc taaccttgag gcaatttgtt ttcttttttc tgccgaaata tcgggcgggc
     4321 atttgctgca gataacaata tcggctctcg acaaggctcg gggagtttct ctcaaattgc
     4381 cggcgggcaa cagaaaatcg tcatacacgg gccgcccgta gtctgtcagc acaatagaaa
     4441 gtcctgcttt caaccttcgg tgctgaaagg catcgtcgag caataccact tggatttcgg
     4501 gtctggcttt gagtaacaac tgcatgcctt ttaccctgtt ttcgtgaaca gctactgtaa
     4561 ttccggggaa tttacctgcc atttgcagcg cttcgtctcc gatatcctct actttgctgt
     4621 cattgcttgc cagtacaaaa cctttggttt ttcgtttgta tcctctgctc agtactgcca
     4681 gcttgtattg tttttgcaac agcttggtca aatattcggt atgaggtgtt ttgccggtgc
     4741 cgcccacact gatgttgcct acgcagatgg tggggatttc gaatgcgctg gttttgcaaa
     4801 tactcctgtc gtagagtaaa ttccgaaaac gcactatccc tccaaaaatc ggggacaatg
     4861 gtaacaaaag aggtcgcgcc gggcgttttg gaggcataga cgattttatt gaataatcag
     4921 gttatagggc atccttgcca aaatacccgg ttctaatttt gtttccatca attccagcaa
     4981 gtcgggggaa tattccgata agtaattttt tattatccgt gtgctgacat ttttgttcat
     5041 gctttccagt actctggtca tcaagtcttt cttttccgga taagtgacta acctgtactt
     5101 ctcaagttca gccaactcaa cggctgcgtc aattgcttga tcaaggtaac ccaattcatc
     5161 taccagcttt ctttctaaag cctgttctcc tgtccaaacc cgaccttcgc caatcaaacg
     5221 gatttggtgg ttttccatgc cgcggccttg tgcgcaacgt tcaacaaaaa gatcgtatcc
     5281 tctttcgacg ctgttctgca tcaatatttt ttcggccgga gtcatttttc tcgaaaaatc
     5341 gccaaaatcg gcgtgttgat tagttttcac catatcgaaa tccagcccga tcttttcggt
     5401 gagttcacgg gcattgaaaa acaggccgaa aataccgata gagcccgtaa gggtggtagg
     5461 agcggcaatt attttgtctg cgttgcacga gatgtaataa ccgcccgaag cggcgtagtc
     5521 gcccatggaa acgactaagg gtttttcttt tttcagttcg ccgagggcat accagatttg
     5581 ttcggaagca aaagcgctgc caccgggaga gttcacccgc aacactactg ctttgacact
     5641 ttcgttttcg cgaagtttgt caatttcgcg aaccagtttt ttgctgctga tgccgtcggg
     5701 agcttcagtg cttattccgc cctgagcgta gagtacagca actttttctt tgatgaattc
     5761 tgtttgtttt tccggaatct tatttaaacc tctgacggaa atcattttga ggtctttaac
     5821 tttgacagaa tcgagtttat cggcaaaaaa ttgcatcatt tccaggggat aaaacaaatc
     5881 gtcaatcaga ccactttcaa ctgtttcttc ggttggttgc agcgacataa aacgatcggc
     5941 cagttggttt agtttatcaa cggatatgtt gcgatctgcg gaaatttcct gcagcatatt
     6001 gttccatatt ccgctgaggt aagacaaggt ctgttctcgg ttttccttgc tcatttcctt
     6061 attaataagg ggctcgacgg ccgatttata tgtgcctacc ttgaagattt cgggctctat
     6121 gccgatcttg tcgagcattt cggtcaaaaa tacgggagtg gcactcatgc ccgcaaaatg
     6181 aagcattccg taaggattta agaataggga atcggctacg ctgcagatat agtactcttt
     6241 ctgattaaag agttcgttgg cataggcata gatgaatttg ccgctttcct tgaattcgct
     6301 cagggctttt ctaatttcct gcgcgctggc tataccacat tgaaggctgc tgctttcgat
     6361 atagatgccg ctgatgtttt cgttgtcttt agcttttttg atggccgcaa gtatttcgtc
     6421 cagcccgatc ttggctgcct ggtccattcc aaaaagttca ttggcgactt cgctaaaggg
     6481 gtcttcctgg gcgtaatcgc ttaggactcc tttgagttcg attttaagca aggtgttgtt
     6541 ctgcaacttg ggcgtagatt ctgtcgaaga ggcaagcccc aacaacacca gcaaccagat
     6601 cagtgacagc accacaaggg caatgacaac tcccagagcc gaagccaggg aaaatttaaa
     6661 aaactgtttc atagcgatac cattagcttg tattgtaaaa aggcatttct gtctaagacg
     6721 aattgtattt ctcgtcttaa gacgaatagt cacaaaagta ctaaaataac ccagggttta
     6781 acaaacaaaa aaagcgattt attttagctt agcccggcat actttcgttt ttccagcgtt
     6841 tttttaaact tgaaaatcag agcaatgaaa aggaatcaaa aaaaattatt acaaaaacaa
     6901 gcaaaataat ttggtgggaa agaattgatg tattaccttt gcacccgctt ttgatggaag
     6961 ataccattgt gatggtggac cggaaatcaa aaacacattt cgttctttga ctgattgaat
     7021 atagtagtac aagagagagc gggcatattt atgtctgatt gtcaatataa taattcttgg
     7081 attgtacgag tcctgagata gacagatttt agttttatat ttttatatat acaacggaga
     7141 gtttgatcct ggctcaggat gaacgctagc gataggctta acacatgcaa gtcgaggggc
     7201 atcacgaggt agcaatactt tggtggcgac cggcgtacgg gtgcgtaaca cgtatgcaac
     7261 cttcctgtaa gtgtg
//
LOCUS       DAOSTD010000084         7244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_9596_length_7244_cov_11.111281, whole genome shotgun sequence.
ACCESSION   DAOSTD010000084 DAOSTD010000000
VERSION     DAOSTD010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9596_length_7244_cov_11.111281"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLI37_06225"
     CDS             complement(<1..608)
                     /locus_tag="PLI37_06225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ58639.1"
                     /translation="MTTKSVQYKGNDKLLFGIILGVLAFWLFAQTTLNINVDMAKDLG
                     MESSMMNIAVSITSLFSGIFIVVFGGLADKIGRVKMVKYGFYFSIIGSLLVGFTPVGG
                     ASGAVLILGRIFQGLSGAAIMPSSLALVKAYWEGAERQRAISLWSMGSWGGSGFAALF
                     GGLMVGIGWRYIFFGAAAISLIGLLMIKDTPESKAEVTDNKKT"
     gene            complement(621..2078)
                     /locus_tag="PLI37_06230"
     CDS             complement(621..2078)
                     /locus_tag="PLI37_06230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ58640.1"
                     /translation="MQTTTYKGTDKVLIGFILAVLTFWLFANSMMNVSPVMTEKLGMD
                     ANTMNIAVSLAALFSGIFIVVIGGLADSIGRVKTFKIGLYLAIAGSLLVGLSPKGSLA
                     VPMLLTGRALQGLSAAFVMPTSLGMIKIFWEGKERQRAISLWSIGTFGGSGLSSLTGG
                     IIASSLGWRWIFFFAVIVAIIALYLTKELPENKREGAGKYKIDWGGIIVFMIAMIALQ
                     IFITNGNKLGWTNWITIVLVAITIVFGYLFVRVEKRSPYAFVDFGLFKNKIFTGATLA
                     NFFINGTSGMLIVSLTLMQRGAQFSAMEAGLLTLGFAIVVILFIRVGEKLLQRFGAKK
                     PIIWGALIICAAIILLMQTQIMTGQYVVLAVIAYSLFGLGLAFFATPATDAALSNLPD
                     SQAGSGAGIFKMASSLGTSFGVAIAAGIYTAVVSRTEPIGWLSNIIGFVGRQDNVVIR
                     QGAMLGLAFCLLLAILVIVSVSTTIPDKKKADNVK"
     gene            complement(2091..2855)
                     /locus_tag="PLI37_06235"
     CDS             complement(2091..2855)
                     /locus_tag="PLI37_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58641.1"
                     /translation="MKRTGQALLLFTLICTAAFAQTNEKIDKSSTWKMVGNAQYVTQH
                     HWRGLGRGPLFGEAPAFEPSISFFNKNWNVGVVAGGSFDGVYKVVMPWISFSPVKNLW
                     IGVWDIYSPGKKLWTNQAKPFDFGLTTSNHFVDAMIFYQLPWLPLTLKWATIVTGKDP
                     NTEGKRNFTTYTELSYAHRWNDFSVWGGVGVTPWKGLYNADKGGVNNLELKLQYDFRL
                     YEPVTMPLFAKFAYSPLSEQFHFVAGASITIPYSFK"
     gene            3083..4243
                     /locus_tag="PLI37_06240"
     CDS             3083..4243
                     /locus_tag="PLI37_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HOQ58642.1"
                     /translation="MNNKLLSEVARLQPEMKEWMDYMHQNPELNMDTPKTAKYIAERL
                     KSWGYDVVEGIGKTGIVASMTVGNGKKSIGMRADFDALPILEDNDLPYKSKIEGWSHL
                     CGHDSHATMLLGAGKYLATTKNFNGTVRLIFQPGEETMEGAPAMVADGLFKRFPVDAV
                     YGMHNMPGLPLGKFHFREGETMAAVDNWEIELTGKGSHGSMPELSIDPLVCGASLVMA
                     LQTIVSRNVSPWKNSVVNVGAFQSGIAGNAVPQKAILRLSIRNMEADLRIMVLDKIRK
                     ITKAQAEAFNCGYEIREGVAGAVLINTPENTKWAAKVAKATFGEDQVVENMHPYMGSE
                     DFAFMLKEKAGTYCMIGNGDTFMVHHPKYVFNQDMLSRGATYWVALAEEYLR"
     gene            complement(4370..>5799)
                     /locus_tag="PLI37_06245"
     CDS             complement(4370..>5799)
                     /locus_tag="PLI37_06245"
                     /inference="COORDINATES: protein motif:HMM:NF027552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOQ58643.1"
                     /translation="RTETPNYSIETALAEEDWDYISFQQASQFSGMYDTYFPHLTNLK
                     SYVEERATNPNVQFVFHSTWAYAQNFNATNFDYYNRDQMTMYNAIVDASHRAAEEAGI
                     SIVVPAGTAIQNGRSSIIGDHFCRDGFHLELTYGRYTAACTWYEKLFNKVVIGNKYRP
                     FTVTAFQAKIAQYAAHYAVENPKKVTSLAYLVDDTEKVPFNNRINLSFGSSTTQGAWN
                     ALESYTVGAGINDLKDIYSDITDVDVVVINRFSGINSEGPTTTATELNMPGDVTKKSF
                     FGNKKQFGGMATPTAVVKFSSLDKNETYDFVLFGSIMNCTDNRETYYKFSGKMPADTI
                     LYLNASNNIKRLVKAVGIAPNESGEIELEVGPGPNNDNSRGFYYITALSFAPHDAASG
                     LDDPLHFSVNAYPNPFDKHLNISPNIDIESVKMLSLDGKVIHSVNHLACGKTHQINTE
                     KIPSGVYIVSITYNGLRDYLKVVKN"
     assembly_gap    5800..5899
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5900..>7244)
                     /locus_tag="PLI37_06250"
     CDS             complement(<5900..>7244)
                     /locus_tag="PLI37_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOQ58644.1"
                     /translation="VESTQDISSNYLAPVKTSTTTSFLPHLNNKTLFVPFDNDRWVRY
                     KAVNFGTSSTSYEVGLVFNATSKQGLVLGSIEHDTWKTGIVISTNAFNDVKSIEVYGG
                     ITSEETRDVLAHGKVKGKKIKSPKIFIGYFDDWRVGMETYGDANAIVAPKLPWNKPKP
                     FGWNSWGALQTNVSYTNSTQVADYIHNNLQNNHFCDTDGVVYMGLDSYWDRLTLTDRI
                     RFVKFCKARGQKGGIYWTPFVDWAKNPDRLVEGSSDVYYRDIYLYANGQPIRTTNIDA
                     YAVDPTHPASKKRAETYISQFKLAKFEYLKLDFLTHGSLEADTYYDDTIYTGIQAYNQ
                     GLKHIVDYLDNSMYINFSIAPLFPANHAHGRRIACDAYAAMTGDMSTEYTLNSLTYGW
                     WLDHVYNYNDADNVVLNGVNIGENRARITSSVITGIFIIGDDYSETGPQIPKLRAP"
BASE COUNT         2023 a         1652 c         1506 g         1963 t
ORIGIN      
        1 gtttttttat tgtctgtaac ttcggctttg ctttcgggtg tatcttttat catcaacaaa
       61 cctataagcg atatggctgc cgctccaaag aaaatatagc gccagccgat gcctaccatc
      121 aatcctccaa aaagagcagc gaatcccgaa cctccccacg aacccattga ccagagacta
      181 atggcgcgct gacgttcggc tccctcccaa taagctttta ccaatgccaa actcgagggc
      241 ataatagccg ctccggagag tccctgaaaa atacgtccta aaatcaacac tgcaccggat
      301 gcaccgccca caggagtaaa cccaaccagc aaagaaccga taatactgaa ataaaacccg
      361 tactttacca tttttactcg accgatttta tcggccaggc caccgaacac cacgatgaaa
      421 atgccggaaa acagcgatgt tatcgacacg gcaatattca tcatcgatga ttccatgccc
      481 aaatctttcg ccatatctac attgatattt agcgtagttt gggcaaaaag ccagaatgcc
      541 agtacaccca aaataatacc aaacaaaagt ttgtcattcc ccttgtactg gactgatttt
      601 gttgtcataa tatgcaaaaa ttatttcaca ttgtctgcct ttttcttatc cggtatggtt
      661 gttgacaccg atacaatcac caaaatcgcc aaaagcagac agaaagccaa accgagcatt
      721 gctccttgtc gaatgaccac attatcttgt ctgcctacga agcctatgat gttgctcaac
      781 catcctatcg gctctgttct ggagactact gccgtgtaaa ttcctgctgc aatggcaact
      841 ccgaagcttg ttccaagcga tgaagccatc ttgaaaatcc ctgctccact acctgcctga
      901 ctgtcgggca agttggataa agcggcatcg gtagccggag tggcaaagaa agccaaaccc
      961 aatccgaaaa gcgagtaagc aataactgcc aacacaacat attgccctgt cattatctgt
     1021 gtctgcataa gcagtataat ggctgcgcaa attattagtg ctccccagat aataggtttt
     1081 tttgcaccaa atctttgcag tagcttttca cccacacgaa taaacaagat aacgacaata
     1141 gcaaaaccta atgtcagcaa cccggcttcc atagccgaga attgtgcccc acgctgcatc
     1201 aatgtaagag aaacgataag catacctgaa gttccgttga tgaagaagtt tgccaaggtt
     1261 gctcccgtaa atattttgtt tttaaacaaa ccaaaatcta caaaagcata gggtgatctt
     1321 ttttcaacgc gaacaaacaa gtagccaaat actattgtaa tggcaactaa aacaatggtt
     1381 atccagtttg tccagcccag tttgtttccg ttggtaatga aaatctgcaa ggcgatcatg
     1441 gcaatcataa acacgattat tccaccccag tcaattttgt atttgcctgc tccttcgcgt
     1501 ttattttcag gaagttcttt tgtcaaatat aaagctatga ttgccactat tactgcaaaa
     1561 aagaaaatcc atctccaacc caggctcgat gcaataattc ctcctgtaag tgagcttaat
     1621 cccgatccac cgaatgttcc gatagaccaa agactgatag cgcgttgacg ctccttgccc
     1681 tcccagaaaa ttttaatcat acccaagctg gtcggcatta caaaggccgc cgaaagtcct
     1741 tgcaaagcgc gccctgtaag aagcatgggc acagcaaggc tacctttggg tgacagtccc
     1801 actaaaagcg agcctgctat agccaaatat aacccgattt tgaacgtttt tacacgaccg
     1861 atgctatcgg ccaatccacc tatcacaact atgaaaatcc ctgaaaacag ggcagcgagc
     1921 gacacggcga tgttcatcgt attcgcgtcc atgccgagct tttcggtcat cacaggcgac
     1981 acgttcatca tggagttggc aaaaagccaa aaagtcaaaa cagctaatat aaagcctata
     2041 agcactttat ctgttccttt gtaagttgtt gtttgcattg ctttgaggtt ttatttgaat
     2101 gaataaggaa ttgttatact tgcacccgcc acaaaatgaa attgttccga aagcgggctg
     2161 tatgcgaatt tggcaaataa gggcatggtt accggttcat acagcctgaa atcgtattgc
     2221 aacttcagct ccaagttgtt gacaccacct ttatctgcgt tgtacagtcc cttccagggc
     2281 gtaacaccca cgccacccca aacgctgaaa tcattccaac gatgcgcgta cgaaagctcg
     2341 gtatatgtgg tgaaatttct ttttccttcg gtattcgggt cttttcccgt aactatggtc
     2401 gcccatttca aagtgagtgg aagccacggc agttggtaga agatcatagc gtccacaaag
     2461 tggttggaag ttgtgagtcc gaaatcgaat ggcttggcct ggtttgtcca caatttcttt
     2521 ccgggagaat aaatatccca aacaccaatc cataagtttt ttacgggcga aaacgaaatc
     2581 catggcatta cgaccttata aaccccgtcg aaagagccgc cggcaacaac acccacattc
     2641 cagttcttat tgaagaagct gatactcggc tcaaaagccg gagcctcgcc aaacagcgga
     2701 cctctgccga gtccacgcca atgatgttga gtaacgtatt gggcattgcc gaccattttc
     2761 catgtcgaag atttatctat cttttcatta gtttgagcaa aagccgctgt acagataaga
     2821 gtaaaaagga gtagtgcttg acctgttcgt ttcatacatt tagttgttga tgtgaataaa
     2881 tatctgtgat attgttcaat ttaactctca tggaaccgat cttgaacatt gaattttttt
     2941 cgcgacaaat gtatggtttt tcctttacga tgtagttaaa aaaataattt tttaattttt
     3001 tttattcgca tttttcgaca agcaatttat gagaataatt ttacctttac gatcgaaaac
     3061 aaacctataa aatttctatg ttatgaacaa taaactatta tccgaagtcg caaggctcca
     3121 acccgaaatg aaagagtgga tggattatat gcaccaaaac cctgaactaa atatggatac
     3181 gcctaaaacc gccaagtata tagccgagag actgaaatcg tggggctacg atgtggtgga
     3241 aggcattggc aaaacaggca ttgtggcatc tatgaccgtg ggcaatggaa agaaatctat
     3301 tggtatgcgt gccgattttg atgcactacc tattttagaa gacaacgatt taccgtacaa
     3361 gagcaaaata gagggttggt cgcacctttg cggacacgac tcacacgcca cgatgttgct
     3421 tggcgcagga aaatatttag caacaaccaa aaactttaac ggaaccgttc gcttgatttt
     3481 tcaaccgggc gaagagacta tggaaggagc accggcaatg gtcgcagacg gactgtttaa
     3541 acgttttcct gttgacgccg tgtacggaat gcataatatg ccgggcttgc cgttaggaaa
     3601 atttcatttc cgcgaaggcg aaaccatggc cgctgtggat aactgggaga ttgaactaac
     3661 cggcaagggt tcgcacggct ccatgcctga actaagtatc gacccattgg tgtgcggagc
     3721 ttcgctggta atggccctgc aaactatcgt aagccgcaac gtatcgccgt ggaaaaactc
     3781 ggtggtaaac gtgggggcct tccagtcggg tatagccggc aatgcagttc ctcagaaagc
     3841 tatcctgcga ttgagcatac gcaatatgga ggccgatctt cgtatcatgg ttctcgacaa
     3901 aatccgcaaa attaccaaag cacaagctga ggcgtttaat tgcggttacg aaattcgcga
     3961 aggcgtagca ggagcagtgt taatcaacac tcccgaaaac actaagtggg cggcaaaggt
     4021 agcaaaagcc accttcggcg aggatcaggt ggtggaaaac atgcacccct acatgggcag
     4081 cgaagacttc gcctttatgc tgaaagagaa agcaggcaca tactgcatga taggcaacgg
     4141 cgataccttt atggtgcacc accccaaata tgtcttcaat caggatatgc ttagccgcgg
     4201 cgcaacctat tgggtggctc ttgcggaaga atatttgaga tagcttcaag cgagtccctg
     4261 agtagtttcg acctcgtgat cacctacccc gcgaggcccc ggaactcgca aacatgcaac
     4321 ccgcaacaaa tagttacagt atgagcgttg tgcggggcag aggggtttat caatttttca
     4381 ccactttcaa ataatccctt agcccattat aagtaatgga aacgatatat accccggatg
     4441 gtattttttc cgtgtttatt tgatgcgttt taccacaggc aagatggttg accgaatgaa
     4501 ttacctttcc gtcgagactc agcattttaa cactctcaat atcgatattg ggactgatat
     4561 ttaagtgttt atcaaaagga ttggggtaag catttacaga aaaatgcagc gggtcgtcta
     4621 gtccggacgc ggcatcgtgg ggagcgaaac ttaaggcggt gatgtagtaa aagccgcgcg
     4681 aattatcgtt gttgggtccc ggcccgactt ccagttcaat ttctccgctt tcgttgggag
     4741 ctattccgac agcttttacc agccttttaa tattattgct cgcgttcaaa taaaggatgg
     4801 tatctgccgg catctttccc gaaaatttgt aataagtttc cctgttgtcc gtgcagttca
     4861 ttatgcttcc aaaaagcaca aaatcatagg tctcgttttt atccaatgag ctaaatttaa
     4921 cgactgccgt tggggtagcc attccgccaa attgtttttt attgccgaaa aaggattttt
     4981 tggtgacatc gccgggcata ttcagttctg tcgcggtagt agtagggccc tctgaattta
     5041 ttccggaaaa gcgattgata actaccacat cgacatcggt tatatcgctg taaatatcct
     5101 tcagatcatt tatcccggca ccgacagtat agctttccaa agcattccag gctccctgtg
     5161 ttgttgaact tccaaaactt aaattaatcc tgttgttgaa cggcactttt tccgtatcgt
     5221 ccaccagata agccaaagaa gtaactttct tcggattttc cacggcgtaa tgagccgcgt
     5281 attgtgctat ttttgcttga aaagcagtaa cggtgaatgg cctgtatttg tttccaatca
     5341 ccaccttgtt aaacaatttt tcgtaccagg tacaagcagc cgtatatctt ccgtaagtca
     5401 attccaagtg aaagccatcc ctgcaaaaat gatctcctat aatgctcgac ctgccgtttt
     5461 gtatggctgt tccggcagga acaacaatgc tgattccggc ttcttcggcc gctctgtgag
     5521 atgcatctac gatggcattg tacatggtca tctgatctcg attgtaataa tcgaagttgg
     5581 tagcgttgaa gttttgagca taagcccatg tggagtgaaa aacaaactgt acgtttggat
     5641 tggttgcgcg ttcctccacg taagatttta aattggttaa atgagggaag taggtgtcgt
     5701 acatacccga gaattggctg gcctgctgaa aagagatata atcccagtct tcttccgcta
     5761 aagctgtttc aatagaataa ttcggcgttt ccgtacgtan nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnng gagctcttag tttgggtatt tgcggacctg tttcactata
     5941 atcatcacca atgataaaga tgcctgtgat caccgaggag gtgattcttg ccctgttttc
     6001 accaatattt acgccgttca acaccacatt gtcggcatcg ttgtaattat atacatgatc
     6061 gagccaccaa ccataggtca gtgaattcaa ggtgtattcc gtactcatat caccggtcat
     6121 agctgcataa gcatcgcacg ctatcctgcg cccgtgtgca tgattggccg ggaaaagcgg
     6181 tgcgatggaa aagtttatat acattgaatt gtccaaataa tctacaatgt gctttaaacc
     6241 ttgattgtaa gcctgtattc cggtatatat cgtatcgtca tagtacgtat ccgcttctaa
     6301 ggagccgtgg gtcaaaaaat ccaatttaag atattcaaat ttggccaatt tgaactgaga
     6361 aatataggtt tcagcccttt ttttactggc gggatgagtc gggtccaccg catatgcatc
     6421 tatattagtt gttcttatcg gttgtccgtt agcatacaaa tagatgtctc tataataaac
     6481 atcactcgaa ccttcgacca atcgatcggg atttttagcc caatccacaa aaggagtcca
     6541 atagataccc cctttttgtc cgcgagcttt acagaatttg acaaacctga ttctgtccgt
     6601 tagtgttaac ctatcccaat aagagtccaa ccccatgtaa accacaccgt ccgtatcgca
     6661 aaaatgatta ttttgcaagt tattatgaat ataatcagcc acttgcgttg agttggtata
     6721 tgaaacattc gtttgaagtg cgccccaact gttccaaccg aagggcttcg gcttattcca
     6781 cggaagcttg ggagctacga tggcattggc atcaccgtaa gtttccattc ctacacgcca
     6841 atcatcaaag tagccgataa atattttggg ggatttaatc tttttccctt ttacctttcc
     6901 atgggcaagc acatctctgg tttcttcaga cgtgatacca ccgtacacct ctatggattt
     6961 cacatcattg aacgcattgg ttgatattac gatgccggtt ttccacgtat cgtgctcaat
     7021 agaaccaagc accaggccct gtttggaagt agcgttaaat accaggccca cttcgtaact
     7081 tgtagatgag gtcccaaagt tgacagcttt atagcgaacc catctgtcgt tgtcgaaagg
     7141 aacaaaaagt gttttattgt tgagatgagg taaaaagctt gtcgttgtcg atgttttaac
     7201 cggtgcaaga taatttgacg atatgtcttg cgttgattcc accg
//
LOCUS       DAOSTD010000085         7067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_9893_length_7067_cov_15.663149, whole genome shotgun sequence.
ACCESSION   DAOSTD010000085 DAOSTD010000000
VERSION     DAOSTD010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9893_length_7067_cov_15.663149"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..3306)
                     /locus_tag="PLI37_06255"
     CDS             complement(1..3306)
                     /locus_tag="PLI37_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOQ58645.1"
                     /translation="MKTIRPLILGFLLISASAAAQTTVTATWPFDRGTDNPSSAQIDN
                     DTAFTLSNFVLGSHLTCTGIQSPNTESVSYSKFQPDAQMGSATPGYELSFMLKPSRGL
                     QFTPTRISFKASRFGTDGGFLVLTRATGEGAEETIAQDLKPNRNNVDPFFSVYDYALE
                     GLPASEDAFVFKIYIHSLGNNKQIGFYNIAVTGRITGELIEVNKYTLNTAVSPAAAGS
                     ITLSPQGNSFDEGSEITLTANKAFGYKFNHWEDGQGTVLSQENPYVHAITADTNLIAV
                     FDKLNTYSLMLAVEGGANDYMLSVSPAGTLVNDTLRYEENTQVSLKASGNKILTFNNW
                     SSGETNSELLLDMTRDYELTAYYSAVDFIVGWDFYRPGNSGRPADFASEGNSTSILIL
                     RNAEGNATGWLDKSQQAAGGYEGRPSAVNWKALADKYYYQLSFDATNFTDIKVASALL
                     FNYNAYSKIKLEYSLDNENFTTIDTIHMLSNKVWYDTEFSLPSDADHAPKVYVRWLPD
                     YTSPVLGTTSNNDGTSITDIYITGTGLIYDDGKAPVFLGSVPADQSTGASATGKIVLS
                     FDEKIKLSDTLYATLDDQPLEGLVSGKTLSFAYRGLEYAREYTFVLPANAVSDLTDNF
                     LDSVITIRFTTMERPVVAKRLFDAVVSNTGELLAALEAAHASQTGERFRILLKNGEYD
                     LGSGITTITAHKISLIGQSRQGVLVYNTSINEGISITSTIFLSGATGFYAQDITFQNK
                     YPYMNTTGRAVVLRDTGTKNIFKNISLLSFQDTYYSGNSGRMYFEDCQINGVVDFICG
                     GSDIFFNRCLLALEKRSGNCITAPSGNGDWGYVFSDCTIDGVDDEARAVVNGTYSLGR
                     PWQGAPRAVYLNTTMKVVPTAAGWTNMSETVLPALFAEFNSVNANGDPVDLSNRMTTF
                     TAGTVSYNPVLTPEQAAQFTIDNVLGGSDAWQPVLATEQAEAPVLTLDAGHLVWDSSD
                     YVLCYVVCRDGEIVDVTTSCTYEIPDGTGAAEFVVHAVNEHGGLSEASNPVQNSGLQS
                     VHSTPEFYASGKTLVLKGVTESVQVTLYDLTGLPVKTLATDSDLELTMKPGAYVLRLQ
                     GLRYDKRAVVVFR"
     assembly_gap    3393..3492
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3493..4900
                     /locus_tag="PLI37_06260"
     CDS             <3493..4900
                     /locus_tag="PLI37_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOQ58646.1"
                     /translation="APDRVQGEIALPASKSISNRALVLYALAGKQSLRTDFADCEDSL
                     LMLNALQSPEAVKDAGAAGTVMRFLTAYYAQTPGEWTLTGTERMKQRPIGLLVDALRQ
                     LGADITYTENPGYPPLLIKGKRLSGGRLSIDGSISSQYITALMLIGPYVDGGLEIELL
                     NKIASAPYIRMTLGLMQAFGAQLSFEGNLIRIAAGAYTAPEEFRVEADWSAASYWFEV
                     LSLAQSGQVLLHGLQADSLQGDAKVMEWFRDFGVQAEFIELPAGKKTSAPAEVVPELI
                     AGKVVTESIAGKALPKSIAGEALPESVSSKIAGSPQAGKPSKRALLLSKTKQQCSFFE
                     ADFSQQPDLAQTFVVTAALRGIPFRIDGLESLKIKETDRLQALIREMLKLGFILEESS
                     PNTLEWQGKKQPAQHRVSIATYQDHRMAMSFAPVALRMKDQNEIKHFEIEDPAVVAKS
                     YPGFWEDLKSCGFTIEYK"
     gene            4897..5340
                     /locus_tag="PLI37_06265"
     CDS             4897..5340
                     /locus_tag="PLI37_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HOQ58647.1"
                     /translation="MTTFIWILFSALVLLGLFSWLLSKHFTGKAQSPADGTDPAEQDV
                     ETDPETDDDVCCGQHLVCEKDSLMYQGEEIVYYDDDELDAYKGKAADAYTGAEIKDFE
                     EVFYSLLESDMAGWLRSLQLREINLPLQLRDEALMIVSERRQNIQ"
     gene            5428..>7067
                     /locus_tag="PLI37_06270"
     CDS             5428..>7067
                     /locus_tag="PLI37_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ58648.1"
                     /translation="MAVSSCSTQKNTWMARNYHALTTHYNIKFNGRESYEAGLKQMEL
                     GHKEDYSSLLPVFPYNEQSASRATGNMNRCIEKCEKAIKTHSIRVKPEKQPERNASEK
                     EKLFYSQEEFNPVMGRVFLLMAKAQFYKADYLSAGSTCSYIIRHFSTDKPLCDEAKIW
                     LARSYYEQEWYYDAENIFYALNDQGFDPTLNALYATSYADFLLRQERIAQALPYLEIA
                     LKNTRKKQEKQRYLFLLAQMHQETGSPAKAYELFKAVPRKNPPYEMALNARIRQTEVY
                     PQGNSRSALKKLKRLSRNPNNQNYLDAIYYAMGNLYFSENDTLKAIDSYNLAIAQSVS
                     NGPYKVQALMTLGDYYYRNELFLEAAPCYQQAKGLIGKEHKETELVEERARVLDLLQP
                     HLETIHLQDSLQWVADLPDEERDKLIEERITQAKKAARQEARLRGQEDALSANRELTQ
                     ANVREEETAPTVLPGMQNTDDSWYFYNPATLETGRREFQRNWGRRRLEDNWRISNKGD
                     FFSHNAFAGIADETTLPGAATDSLPEDALRGEGLLSDT"
BASE COUNT         1827 a         1828 c         1792 g         1520 t
ORIGIN      
        1 ttatctaaac accaccaccg ccctcttatc gtaacgcaag ccctgcagcc taagcacata
       61 agcacccggc ttcattgtca gttcaagatc ggagtccgtt gcaagggttt tcaccggcag
      121 tcccgtcaaa tcgtacaaag tgacttgtac actttcggtc actcccttca gcaccaaggt
      181 tttgcccgag gcataaaatt cgggagtcga atgtaccgac tgtaaaccac tgttctgtac
      241 gggattggaa gcttcgctca gtccgccgtg ttcgttgacc gcatgcacca caaattcggc
      301 agcacccgtg ccatcgggaa tctcataagt gcaggaagtg gtaacatcca ctatctcgcc
      361 gtcgcggcaa accacataac agagcacata atcgctggaa tcccaaacca aatgccccgc
      421 atccaatgtc agaacaggag cctctgcctg ttcggtagcc agcaccggct gccaggcatc
      481 cgatccgccc aatacattgt cgatggtaaa ctgagccgcc tgctcgggag taagtaccgg
      541 attgtacgac accgtaccgg ccgtaaacgt ggtcatacga ttactcaaat caaccggatc
      601 gccgttggca ttcacactat tgaattccgc aaacaaagcc ggcaaaacgg tttcgctcat
      661 attagtccaa ccggcagccg tgggcactac cttcatggtc gtgttgaggt aaacagcccg
      721 cggagctccc tgccagggac gtcccaaact gtacgtgccg ttcaccaccg ccctggcttc
      781 gtcgtctacg ccatcgatgg tgcaatcgct aaagacatag ccccaatcgc cgttgcccga
      841 aggagccgta atgcaattgc cgctgcgttt ttccaaagcg agcagacaac ggttgaagaa
      901 aatatcgctg ccgccacaaa taaagtcgac caccccgttg atctggcaat cttcgaaata
      961 catccggccg gaattgcccg aataataggt atcctggaaa ctgagcagac tgatgttttt
     1021 gaaaatattc ttcgtgccgg tatcgcgcaa caccaccgcc cggcccgtcg tattcatgta
     1081 aggatattta ttctggaaag taatgtcctg ggcataaaat cccgtggctc cactcaggaa
     1141 aatggtggaa gtaatgctta tgccctcgtt gatggaagta ttgtaaacca ggacaccctg
     1201 tcggctctgc ccgatgagcg aaatcttgtg ggccgtgatg gtagtgatac ccgaacccag
     1261 gtcgtattcc ccgtttttga gcaggatgcg gaaacgttcg cccgtctgcg aagcatgtgc
     1321 cgcctcaagt gccgccagca attccccggt attggatacc acggcatcga aaagcctctt
     1381 agcgaccaca gggcgttcca tagtggtgaa acggatggta atcaccgaat cgaggaaatt
     1441 atccgtcagg tccgataccg cattggcggg gagaacaaaa gtgtattccc tggcgtactc
     1501 caggcccctg taagcaaaac tcagggtttt tccgctgacc aggccttcca gaggttggtc
     1561 atcgagagtg gcatacaagg tatcggacag cttgatcttt tcgtcaaaac tgagtacgat
     1621 tttcccggtt gccgaagccc cggtcgattg gtcggccgga accgaaccca ggaaaaccgg
     1681 agctttgccg tcgtcgtaga tcaggcctgt tcccgtgata taaatatcgg tgatcgaagt
     1741 gccgtcgttg ttggatgtgg tgcccaacac aggcgaagta taatcgggca gccagcgcac
     1801 atacaccttg ggtgcatggt cggcatcgga gggcagactg aattcggtgt cgtaccatac
     1861 cttgttgctc aacatatgaa tggtatctat agtggtgaaa ttctcattat cgagcgaata
     1921 ttccagtttg attttggaat aggcattgta attgaacaaa agcgccgaag ctacttttat
     1981 atccgtgaaa ttggttgcgt cgaagctgag ctggtaataa tacttgtccg ccagagcttt
     2041 ccagttcact gccgaggggc ggccttcgta acctccggcg gcctgctgcg acttgtctaa
     2101 ccagccggtg gcattaccct cggcgttgcg gagtatcagg atggaagtgc tgttgccttc
     2161 ggaagcaaaa tcggcgggac ggccgctgtt gccgggacgg taaaaatccc agcccacaat
     2221 gaaatctact gcgctgtagt aagccgtcag ttcgtaatca cgggtcatat cgagcagcaa
     2281 ttcgctgttg gtttcacccg agctccaatt gttgaaggtc aagattttgt ttcccgaagc
     2341 tttgaggctc acctgtgtat tttcttcgta acgaagggta tcgttgacaa gggtgcctgc
     2401 aggcgaaacg gaaagcatgt aatcgttggc gccgccctcc acggctagca tcaggctgta
     2461 ggtattgagt ttgtcaaaaa cggcaatcag gttggtatcg gccgtaatgg catgcacata
     2521 gggattctct tgcgacagga cggtaccctg accgtcttcc caatggttga atttgtaacc
     2581 gaaagccttg ttggcggtca gggtgatttc ggaaccttcg tcgaaggaat taccctgggg
     2641 actcagcgtt atgctgccgg ctgcggcagg agaaacggct gtgttcagcg tatatttgtt
     2701 cacttcgatc agttcgcccg taatccggcc tgtaacggca atgttataga agccgatttg
     2761 cttgttgttt cccaggctgt gtatgtaaat cttaaataca aaagcgtcct ccgaagccgg
     2821 cagaccttcc aaggcataat cgtacaccga aaagaagggg tctacgttgt tgcgattggg
     2881 ttttaaatcc tgggctatcg tttcttctgc cccttcgccg gtggccctgg tcaacaccaa
     2941 aaaacctccg tcggtaccaa agcgcgaagc cttgaaacta atgcgggtag gtgtaaactg
     3001 caatcccctc gagggcttga gcataaaact aagctcatag cccggggtgg ccgaacccat
     3061 ctgggcatcg ggctgaaatt tggaatacga aacgctttcg gtattgggcg actgtatgcc
     3121 ggtgcaggtc aggtgcgaac ccaaaacgaa attgctcaat gtaaatgccg tatcgttgtc
     3181 gatttgggca gacgaaggat tgtctgtacc ccggtcaaaa ggccaggtgg ccgttaccgt
     3241 ggtttgggcc gcagccgatg ccgagatcag caagaagccc agaataaggg gtctgattgt
     3301 tttcatatct gttagtaaat aaggctaata aagaggcgaa agtaactctt ttttagaaaa
     3361 aacaagaaag atcggttgtt aatcgtatca aannnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnagctcccg atcgtgtaca gggagaaatt gccttgcccg cttccaaaag
     3541 catcagcaac agggccctgg tgctctatgc cctggccggc aaacaaagcc tgagaaccga
     3601 ttttgccgat tgcgaagaca gcctcttgat gttgaacgct ctgcaaagcc ccgaagccgt
     3661 caaggacgcg ggtgcagcag gtaccgttat gcgctttttg acagcctatt acgcacaaac
     3721 tcccggagaa tggacgctta cgggaaccga aagaatgaaa caacgcccca taggtttact
     3781 ggtggatgct ctccgccaac tgggagcgga catcacctac acggaaaatc cgggctaccc
     3841 tcccctgctg atcaagggca aacggctcag cggcggacgc ctgtccatcg acggaagcat
     3901 cagcagccag tacatcacgg ctttgatgct gatagggcct tacgtcgacg gcggcctcga
     3961 aatagagttg cttaataaaa tcgcctcagc tccctatatc cgtatgacac ttggtctgat
     4021 gcaggccttt ggggcccaac tgagtttcga aggcaatctt attcgcatcg cagcgggtgc
     4081 ttacaccgct cccgaagagt ttcgggtgga agccgactgg tcggccgctt cgtattggtt
     4141 cgaagtgttg agcctggccc aatcggggca agtcctgctg cacggcctgc aagccgacag
     4201 cctgcagggc gacgccaagg tgatggaatg gttccgggac tttggagttc aggccgagtt
     4261 tatcgaactt cccgccggga aaaagacctc tgcgcccgcg gaagtcgtgc cggaattaat
     4321 tgccggaaaa gtcgtgacgg agtcaattgc cggaaaagcc ctgccgaagt caattgccgg
     4381 agaagccctg ccggagtcag ttagcagcaa aatcgccggc agcccgcagg caggaaaacc
     4441 gagcaaacga gccctactcc tgagtaagac caagcagcaa tgctccttct tcgaagcaga
     4501 cttcagccaa caacccgatc tggctcaaac ttttgtagta acggccgcct taagggggat
     4561 tccttttcgc atcgacggac tggaaagcct gaaaataaaa gagaccgacc gcttgcaggc
     4621 cctgattcgg gaaatgctga aactgggctt tatactcgaa gagagcagcc ccaatacgct
     4681 ggaatggcag ggcaaaaagc agcccgcaca gcaccgcgtc agcatagcca cctaccaaga
     4741 ccaccgcatg gccatgtcgt ttgctcccgt ggcactccga atgaaagacc aaaacgaaat
     4801 aaaacatttc gaaatagaag atccggccgt ggtcgccaaa tcttaccccg gattttggga
     4861 agatctcaaa agctgtggtt ttactataga atacaaatga caacgtttat ctggatatta
     4921 tttagcgctc tggtgcttct gggtttattc agttggctgc tgagtaaaca tttcaccggc
     4981 aaagctcagt cgcccgcaga cggaacagac ccggcggaac aagacgtgga aacagacccg
     5041 gaaacggacg acgacgtctg ctgcggacaa catctcgtgt gcgaaaaaga cagcctgatg
     5101 taccagggcg aagaaattgt ctattacgac gacgatgagc tggatgctta caagggtaaa
     5161 gcggcagatg cctataccgg agcagagatc aaagacttcg aagaagtatt ttacagcttg
     5221 ctcgaaagcg atatggccgg ttggttgcgc agcctgcagt tgagagaaat aaacctgcct
     5281 ttgcagttga gagacgaggc cctgatgatc gtaagcgaaa gaagacaaaa catacaataa
     5341 aaccggcaaa aagctgttta ataaacgatc ggttcttgag tattgaaagc acaattttca
     5401 catatccttt tgctaagcct gctgctggtg gcagtctctt cttgttccac ccagaaaaac
     5461 acctggatgg cccggaatta ccacgccctg accactcatt acaacatcaa attcaatggg
     5521 cgggaaagtt acgaggcggg gctgaaacaa atggagctcg gtcacaaaga ggattattcc
     5581 tcgctgctgc ctgtgtttcc ttacaacgag caatcggctt cacgggcgac aggcaatatg
     5641 aatcgctgca tagaaaaatg tgaaaaggcc atcaaaaccc attccatacg cgtaaagccc
     5701 gaaaaacaac cggaacgcaa cgccagcgag aaagaaaagc ttttttacag tcaggaagaa
     5761 ttcaaccctg tcatggggcg ggtattttta ctgatggcca aggcccagtt ttacaaagcc
     5821 gattacttgt cggcgggttc tacctgttcc tatatcatcc ggcatttttc caccgataaa
     5881 ccgctctgcg acgaagccaa aatctggctg gccagatcgt attacgaaca ggaatggtat
     5941 tacgatgcgg aaaatatttt ttacgcactc aacgatcagg gtttcgatcc cacgctcaac
     6001 gccttgtatg ccacttctta cgccgatttt ctactgaggc aggaaagaat cgcccaggcc
     6061 ttgccctatt tggaaattgc cctaaagaac acccgcaaaa aacaggaaaa gcaacgctat
     6121 ctttttttgc tggcccaaat gcatcaggaa acaggctctc cggccaaagc ttatgaactc
     6181 ttcaaagcag tgccgcgcaa aaatccgcct tacgaaatgg ccctgaatgc cagaataagg
     6241 caaacggagg tctatccgca aggcaacagc cgatcggctc tgaaaaaatt aaaaaggctc
     6301 agccgcaatc ccaacaacca aaactatctg gatgccatct attacgccat gggcaacctg
     6361 tacttttcgg agaacgatac gctgaaagcc atcgacagct acaaccttgc cattgcgcaa
     6421 agcgtttcca acgggcccta caaagtccag gccctgatga ctttgggcga ttattattat
     6481 cgtaacgaac tctttctcga ggcagctccc tgttatcagc aagccaaagg cctgatcgga
     6541 aaggagcaca aagagacgga actggtcgaa gaaagagccc gggtgctgga tctgcttcag
     6601 ccccacctgg aaaccataca tctgcaggac agcctgcaat gggtggccga cctacccgat
     6661 gaggaacgcg ataaactgat agaagaacgg attacccagg ccaaaaaggc agcccgccag
     6721 gaggccaggc ttcgcggtca ggaagacgct ctgagtgcca accgggagct gacccaggcc
     6781 aatgtgcgtg aagaagaaac agcccccacc gtcttgcccg gcatgcaaaa caccgacgac
     6841 agttggtact tttacaatcc agccactctg gagaccgggc ggcgggagtt tcaacgcaac
     6901 tggggcagac gcagactcga agacaattgg cgtataagca acaaaggcga ctttttcagc
     6961 cacaatgctt ttgccggaat cgctgacgaa accactttgc ccggggccgc caccgacagt
     7021 ctgccggaag atgctctgag aggcgaaggt ctgctttccg acacgga
//
LOCUS       DAOSTD010000086         7026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_9960_length_7026_cov_13.449433, whole genome shotgun sequence.
ACCESSION   DAOSTD010000086 DAOSTD010000000
VERSION     DAOSTD010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9960_length_7026_cov_13.449433"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLI37_06275"
     CDS             <1..317
                     /locus_tag="PLI37_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58649.1"
                     /translation="YLIFYFLLLSLVRWKIKTSIFSWKQAQVVLLVLLLFLCDRLWTS
                     FLTPLFLKLPLKVVYAQVIDAVVKSSILLGSGVLLTYLWRISEDANTLIRNILNRFRL
                     RK"
     gene            complement(318..1553)
                     /locus_tag="PLI37_06280"
     CDS             complement(318..1553)
                     /locus_tag="PLI37_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOQ58650.1"
                     /translation="MYRLLFSLYVALFHIVALFYKKAGKIVKGQRDSWDILKNLNPKK
                     NYLWFHASSLGEFEQGRPLMEAITRTYPRYSVLLTFYSPSGFEVQKNYRGADAVCYLP
                     FDTMCNVRRFIELVQPKAAFFIKYEFWPHYLKELNKEGVPVYLVSGVFRKNQLFFKPW
                     GGFYRRLLKYFSAFFVQNETSKVLLNSIGVNNKVFITGDTRCDRVIDIATHAPEQELI
                     KEFAHDSMVLVAGSTWPEDEEILIPYINNVHTEWKLIIAPHEIDENRLRGIEKLLNRP
                     FVRYSTMKNEDAGQVECIIIDSFGLLSSVYRYGQAAYIGGGFGVGIHNTLEAAVYGIP
                     VLFGPNHTKFEEALALIEAGAAFCVNSQNDLEDKLDTFLASNKTLTQAGENARNYVRE
                     KSGGTAKILDVLKTELFKA"
     gene            complement(1584..3107)
                     /gene="gltX"
                     /locus_tag="PLI37_06285"
     CDS             complement(1584..3107)
                     /gene="gltX"
                     /locus_tag="PLI37_06285"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOQ58651.1"
                     /translation="MSESNTVRVRFAPSPTGPLHIGGVRTALYNYLFARHAGGTMILR
                     IEDTDSKRYVPGSEAYIVEALQWLNIQIDEGVSQGGNYGPYRQSERKEIYKKYIDQLL
                     EQDKAYLAFDSPEQLEAKRAEDPNFQYDASSRLSMINSLTLSATQVEERLQGGEDYTV
                     RFKIEPDRSIEVQDMIRGRVVFNSSVLDDKVLYKSADQLPTYHLANVVDDHLMKISHV
                     IRGEEWLPSTPLHVLLYEALGWDKQMPLFAHLPLLLKPDGKGKLSKRDGDRLNFPVFP
                     LNWEDPQSGEISKGYREAGYFPQAIINFLALLGWNPGNNREMFSMDQLIEAFTIERCS
                     KSGAKFDYEKARWFNHQYMQELDNSTLAEILLPLIEAKGYKTSRGFVEAILVHIKERM
                     VFPQDFWDQAAYFFVAPDQYDAKTVAKRWKPESAAQLTELGDLLERISDYSDAEAIES
                     EVKAWIEQKSYKMGLIMNAFRLALVGEGKGPGMFDIVCLLGKEETLQRLRKAIKTLG"
     gene            3239..5332
                     /locus_tag="PLI37_06290"
     CDS             3239..5332
                     /locus_tag="PLI37_06290"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOQ58652.1"
                     /translation="MAKFKPKLNTASTIFKIFLFVVAAGLILYAFPREGRFRYQFQEG
                     KPWRYGLLTAPYSFPVIKDQKELAAEKDSLLRTHKPYVVVDATVSTEMIGNFNRNYDL
                     YLRATVPPRYKTYINDQLALVYGAGIISASVRNEFQKEKLKSLLMVTKPIAETRDLSS
                     VFTIKEAYSAILSNLPARIHRDTLVRICKIEEYLRENLSYDRETSEQSKEAMIGELSE
                     TSGLVQRGERIIDKGEIVNAITFLKLSSLKKAAEERNINSTENWVLTGQIIWVAGLFL
                     MLFLFIRYFTPEIYNKTHNILFLLILVTSLSLLSALVERNSTLNLYMIPYAILPVIIC
                     TFFNARTAYFTHLITVLICSVIAPFPFEFLTLQLTVGMAVVLSLKELTQRSQLFLCIV
                     WSFVCYSVFYTGIAFIQEGAPNQLQWQMYVLFAINAVLMFTAYLLIYLFEWIFGYTSN
                     VTLMELSNINSPLLRLFSESTPGTFQHSMQVSNLAAAAAQEINANAQLVRTGALYHDL
                     GKMQNPAYFTENQANGQNPLDQMETEKAAKIIVDHVNNGLKLANQYHLPKVVKDFIAT
                     HHGTGPTRYFYTIYKNEHPGQEPPKEAFYYPGPNPFSKETAILMMADTIEAASHSLTE
                     YSESSISELVEKLIGRQIQEGAYKNVPITFQELEIIKSVFKQKLKSIYHTRIAYPEEK
                     KRVVRNTSDKKTNRK"
     gene            complement(5335..6300)
                     /locus_tag="PLI37_06295"
     CDS             complement(5335..6300)
                     /locus_tag="PLI37_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOQ58653.1"
                     /translation="MKKNYSICVLDAFTSNPGDLSWDALRQFGELQVYERSPEEKVIE
                     RAKDADILLTNKVILDEDLIKKLPRLKYIGVLATGYNVVDIHAAKAHNIVVTNIPAYS
                     TDSVAQMVFAHVLNIYNQVQYHAQEVKKGRWSGCRDFSFFDKPLMELKGKTMGIVGLG
                     RIGMATAQLALAFGMEVIALTSKTKDDLPAGISPVSEDELFRKSDVLSLHCPLTPENK
                     GFVQKEKLALMKTGAILVNLSRGPLVNEEDLAWALNNDRLYAAAVDVLSKEPPCSDNP
                     LLAAKNCYITPHIAWATKEARARLIAQAVENVRAFVEGHPINRID"
     gene            complement(6337..6930)
                     /gene="folE"
                     /locus_tag="PLI37_06300"
     CDS             complement(6337..6930)
                     /gene="folE"
                     /locus_tag="PLI37_06300"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOQ58654.1"
                     /translation="MIDKRLILGTEPNPVQDEIAGHYLEILRLLGEDTEREGLLKTPK
                     RIAKAMQFLTYGYQLDPEEVLRLAKFHEEYRQMVIVRDIDFYSLCEHHMLPFYGKAHV
                     AYIPNQHITGLSKIARVVDIFSRRLQLQERMTTQIKECIQKTLNPLGVMVVIEAQHLC
                     MQMRGVEKQNSVAVTSDFTGVFNQAKTRQEFMNLIGR"
BASE COUNT         1923 a         1657 c         1452 g         1994 t
ORIGIN      
        1 cgtatctgat tttttatttt ttgttgctta gtttggtgcg ctggaaaata aaaacctcca
       61 ttttttcatg gaaacaagct caggttgtct tattggtttt gttgctgttc ttgtgcgacc
      121 ggttgtggac gagcttcctg acccccttat ttttgaaatt acctctaaag gtagtttatg
      181 ctcaagtgat cgacgctgtc gttaaaagct ctatattgct cggttcaggt gttttattga
      241 cttatttatg gagaatttcc gaagatgcca atacgctcat aagaaacatt ctgaaccgct
      301 tccggttgag gaagtaacta agccttaaac aattcggttt tcaggacatc cagaatttta
      361 gcggtgcctc ccgatttttc tcttacataa ttccgggcat tttctccggc ctgagtcaga
      421 gtcttgttgc tcgcgaggaa tgtatccaac ttgtcttcca aatcattttg agaatttacg
      481 caaaaggcgg ctcctgcctc gatcaaagcc agcgcttctt cgaatttagt atgattggga
      541 ccaaacaaaa ccgggatgcc ataaacggca gcttccaaag tgttgtgtat gccgactcca
      601 aaaccgcccc ctatatatgc tgcctgcccg taacgatata ccgaagacaa tagtccaaaa
      661 ctgtcgataa tgatgcattc aacctggcct gcgtcttcgt ttttcattgt cgaatagcgt
      721 acaaagggcc gattcagaag cttttctatc cccctaagac gattttcgtc aatttcatgc
      781 ggagcaatga tcagtttcca ttccgtgtgt acattattta tgtagggaat tagtatttct
      841 tcgtcttcgg gccaggtgct gccggcgacc aacaccatag aatcatgggc aaattccttt
      901 atcagttctt gttcgggagc atgagtcgct atgtctataa cgcggtcgca gcgggtatcg
      961 ccggtgatga aaaccttgtt gttcacacct atagagttaa gtaagacctt ggaagtttcg
     1021 ttttgaacaa agaaagcact gaagtatttg agcagtctac gataaaatcc accccaaggt
     1081 ttaaaaaaca actggttctt gcgaaacacg ccggaaacaa ggtaaacagg aacaccctct
     1141 ttatttaatt ctttcagata atggggccag aattcatact taataaaaaa ggcagctttg
     1201 ggctgtacca attctataaa acgcctgacg ttacacatcg tatcgaaagg caggtaacaa
     1261 accgcatcgg ctccccggta gtttttttgc acctcaaaac ccgagggtga ataaaaagtc
     1321 aacaagacag aataccgggg atacgtacgg gtaatagcct ccatcaacgg gcgtccctgt
     1381 tcaaactcgc ccagcgaaga ggcatgaaac caaagataat tctttttggg attcaggttt
     1441 ttcaagatat cccaactgtc tcgttggcct ttgactattt taccggcttt tttgtaaaaa
     1501 agcgcaacta tatggaacaa agctacatac agcgaaaaaa ggagacgata cataataagc
     1561 agattaatta acaacaaatc gtactagccc agcgttttta tggctttacg taaacgctgt
     1621 agcgtttctt ctttccccag caggcagacg atgtcaaaca tgcccggacc cttgccttcg
     1681 cccaccaagg ctaagcggaa ggcattcata atcaaaccca ttttgtatga tttttgttct
     1741 atccaggctt tcacttcgct ttcgatggct tcggcatcgg aataatcgct gatgcgttcg
     1801 agcaagtcgc ctaattcggt aagctgggct gcagattcgg gcttccaacg tttggccacc
     1861 gtcttggcat cgtattgatc gggggcgaca aaaaaatagg cagcctgatc ccaaaaatct
     1921 tgcgggaaaa ccattctttc cttaatatgg accaaaatgg cctcgacaaa accgcgagat
     1981 gttttgtatc cttttgcttc tatcaagggt aacaaaatct ctgccaaggt actgttgtcc
     2041 agctcctgca tatattgatg gttgaaccag cgggcttttt cgtaatcgaa cttggctccg
     2101 cttttgctgc aacgttctat ggtaaaagct tctatcaatt gatccatcga aaacatctcc
     2161 ctattgtttc cgggattcca gcccaacaac gcgagaaaat tgatgattgc ctgggggaaa
     2221 tagccggctt cgcgataacc tttgcttatt tctccgcttt gaggatcttc ccagttcagg
     2281 ggaaaaaccg gaaaattcag acggtctccg tcacgcttgc tcagtttccc tttgccatcg
     2341 ggtttgagca acaagggtaa atgagcaaag aggggcattt gtttgtccca accaagggct
     2401 tcgtacagta aaacatgcaa aggggtggag ggcagccatt cttctcccct gatcacatgg
     2461 ctgattttca tcaaatgatc gtccaccaca ttggccaggt ggtaggtggg caattgatcg
     2521 gccgacttgt acaaaacctt gtcgtccaga acactcgaat taaaaaccac ccttcctctg
     2581 atcatatctt gcacctctat gctacgatcg ggttctatct tgaaacgaac ggtataatct
     2641 tcacctccct gcaatctttc ctcgacttgg gtagcagaca aagtaagtga gttgatcatg
     2701 ctcaatcggc ttgaagcatc gtattgaaaa ttagggtctt ctgctcgttt tgcctcaagc
     2761 tgttcgggag aatcaaaagc cagataggcc ttgtcttgtt cgagcaattg atctatgtat
     2821 tttttgtaaa tttctttccg ttccgattga cggtaagggc catagttgcc tccctgcgaa
     2881 acaccctcgt caatttggat attgagccat tgcagtgctt ccacaatgta tgcttcggat
     2941 ccgggtacat aacgtttgct gtcggtatct tctatgcgta aaatcatggt gccgccggcg
     3001 tggcgggcaa ataaataatt ataaagagcg gtccttacgc ctcctatgtg cagaggaccg
     3061 gtagggctgg gagcaaaacg gacacggaca gtattggatt cagacataac acaaacattt
     3121 tgagggcaaa agtacaagat atttttaaaa ttcggcctta gttattgccg ccaagggttt
     3181 tttttgtaat tttcgcccct gttcacaggc ttccggccga aaaatcaaat tgaaagtcat
     3241 ggctaaattt aaacctaaac taaatactgc atccacgata ttcaaaatct tccttttcgt
     3301 tgttgcagcc ggcctgatcc tttacgcttt ccccagagaa ggcagattcc gctatcagtt
     3361 tcaagaaggc aaaccttggc gctacggctt gctcacggct ccctacagtt tccccgttat
     3421 taaagaccag aaagaactgg ctgccgaaaa agacagtctg ttgaggacgc acaaacctta
     3481 cgttgtagtg gatgcaacgg tctcaacaga aatgattggt aacttcaacc gcaattacga
     3541 cttgtactta agagctaccg tacccccacg ttacaaaact tatataaacg atcaacttgc
     3601 cctggtctat ggagccggta tcatttcggc ttccgtccga aacgaattcc aaaaggaaaa
     3661 gttgaaatcg ctgctgatgg ttactaagcc tatagccgaa acccgcgatc tgtcttcggt
     3721 tttcactatt aaagaagcct attctgccat tttgagcaac ttgccggcac gtatacatcg
     3781 agatacctta gtcagaatat gcaagataga ggaatacctc cgggaaaacc taagctatga
     3841 tcgggaaact tctgaacaat cgaaagaagc aatgattggt gagctgtctg aaacctcggg
     3901 attggtccag cgaggagaac gcatcattga taaaggcgaa attgttaacg ctataacttt
     3961 tctaaaactg tcttcattaa aaaaggcagc agaagaacgt aatataaatt caacggaaaa
     4021 ctgggtgctg accggccaga ttatatgggt ggccggcctg tttttgatgc tgttcctatt
     4081 tattaggtat tttacacccg aaatctacaa caaaacccac aacatcctct ttttactgat
     4141 tcttgtcaca agtctgagtt tattgagcgc cttagtggag agaaacagta ccttgaatct
     4201 ttatatgatt ccctatgcca tactgcctgt gataatctgt accttcttca atgccagaac
     4261 ggcttatttc acccatctca tcaccgtatt aatctgttct gtcattgctc cctttccctt
     4321 tgagttttta acgctgcaac tcactgttgg tatggctgtt gtcctctcac tcaaagagct
     4381 tactcagcgt tcacaattgt ttttgtgtat cgtctggagt tttgtctgct attcggtatt
     4441 ttacaccggc atagctttta ttcaagaggg agcgcccaat caacttcaat ggcaaatgta
     4501 tgtccttttt gccattaacg cagtactgat gttcaccgcc tacctcctga tatatttgtt
     4561 tgaatggata ttcggctata cttccaatgt cactctaatg gagctctcca acattaactc
     4621 ccccttgctc cgcttgtttt cggaaagcac tccgggcact ttccaacatt ccatgcaagt
     4681 ttccaacctg gctgccgccg ccgctcagga aataaacgcc aatgcacaac tggtaagaac
     4741 aggggccttg tatcacgacc tgggcaaaat gcaaaatccc gcctatttta ccgaaaacca
     4801 ggcaaacgga caaaatccct tagaccaaat ggaaacagaa aaagcagcca aaataatagt
     4861 ggatcatgtc aacaatggtc tcaaactggc caatcaatac cacctgccca aagtggtgaa
     4921 agactttatt gctactcatc acggcaccgg accgactcgc tatttctaca ctatctacaa
     4981 aaacgaacat cccggccagg agcctccaaa agaagcattt tattatccgg gcccaaatcc
     5041 tttttctaaa gagacagcta ttctgatgat ggccgatacc attgaggccg cttcgcatag
     5101 tttaacggaa tattccgaaa gcagcatttc agaactggtg gagaagctga tcggccggca
     5161 aattcaggaa ggggcttata aaaatgtacc cattaccttc caggaattgg agatcattaa
     5221 atcggtcttt aaacaaaaat taaaaagcat ttatcataca cgaatagcct atcctgaaga
     5281 aaagaagagg gttgttcgta acacttcgga taaaaaaact aacaggaagt agaattaatc
     5341 gattcggtta atcggatggc cttccacgaa ggctctgacg ttttcgactg cctgggcaat
     5401 caaacgcgcc cgggcttctt tggttgccca ggcaatgtga ggggtgatgt aacagttctt
     5461 agctgccagt aaggggttgt cggagcatgg aggttcttta gataatacat caacagctgc
     5521 tgcatagagt ctgtcgttat tcaaagccca agcaaggtct tcttcgttaa ccaatggccc
     5581 ccggcttaaa tttaccagta tagctcctgt tttcattaaa gccaattttt ccttttgcac
     5641 aaaacccttg ttttcgggtg ttagggggca atgcaggctc agcacatcgc ttttgcgaaa
     5701 caattcatct tcactgaccg gagaaatacc ggcaggtaaa tcgtctttgg tcttgcttgt
     5761 caatgcaata acttccattc caaaagccag ggccaattgg gcagtagcca ttcctattct
     5821 acccaaaccc acaataccca ttgttttgcc tttgagttcc atcaaaggtt tatcgaaaaa
     5881 agaaaaatca cggcaccccg accaacggcc ttttttcact tcctgcgcgt gatattgtac
     5941 ttggttgtat atgttcagta cgtgagcaaa caccatctgg gccaccgaat cggtgctgta
     6001 agcaggaata ttggtcacca ctatgttgtg ggcttttgca gcgtgtatgt caactacatt
     6061 ataaccggta gccagtactc cgatatactt aagccttggc agttttttta tcagatcctc
     6121 gtccagaatc actttgttag tcaacaaaat gtcggcatct ttcgcccgct caataacttt
     6181 ttcctcggga ctgcgctcgt acacctgcaa ttccccgaat tgacgaagag catcccagga
     6241 caagtctccg ggattggagg taaaagcgtc caacacgcaa atggaataat tctttttcat
     6301 ggccttttat tcaacagcaa actgcattgg agctacttac cttccgatca gattcataaa
     6361 ttcctgcctg gtctttgcct ggttaaagac accggtaaaa tcggaagtaa cagcaactga
     6421 attttgtttt tcaactcccc gcatttgcat acaaagatgt tgtgcctcaa tcacaaccat
     6481 cacccccaga ggattgaggg ttttctgaat gcattctttg atctgcgtag tcatgcgttc
     6541 ctgcaactgc aagcggcgtg aaaaaatatc caccacccgg gcaatcttac tcagacctgt
     6601 aatatgctga ttaggaatat aagccacatg ggctttaccg taaaagggca gcatgtggtg
     6661 ttcgcacaag gaataaaaat ctatgtccct gacaataacc atttgccggt attcctcatg
     6721 aaatttggcc aatctcaaca cttcttcggg atccaactga taaccgtaag taagaaattg
     6781 catggctttg gctatgcgct tcggtgtttt caacaaacct tcacgttccg tgtcttctcc
     6841 cagcaaacgt aaaatttcca agtaatgtcc ggctatttca tcctgaacag gattaggttc
     6901 agttccaaga attaatcgtt tatctatcat agacttagtt ctttatcccg actttaagta
     6961 taatagcttt ctcagctaag gttcaacctg tattttttcg cgaactatcg acatttcgcg
     7021 tccgaa
//
LOCUS       DAOSTD010000087         6950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_10069_length_6950_cov_7.649601, whole genome shotgun sequence.
ACCESSION   DAOSTD010000087 DAOSTD010000000
VERSION     DAOSTD010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10069_length_6950_cov_7.649601"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..706
                     /locus_tag="PLI37_06305"
     CDS             137..706
                     /locus_tag="PLI37_06305"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOQ58655.1"
                     /translation="MKKVVSKGIGGYSFQIEEDACRILQDYIEQFRSKLGEAEDVSEI
                     MDDIEARIAEILREEITSPNQVVGEGLIRQVVARMGMPDGSNDFTEYHVPYASNKRAA
                     RRLFRNPDDRKIGGICGGLGAYLGVDSTLLRILFLIGFFLGLLSFWVYLILWICVPDA
                     RTPAQKLQMQGLPVTAENLLRYSAKQSEK"
     gene            715..1050
                     /locus_tag="PLI37_06310"
     CDS             715..1050
                     /locus_tag="PLI37_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOQ58656.1"
                     /translation="MNTDNVKAQMRKGVLEYSILSILSRGDEYASSLIKILKDAQMIV
                     VEGTLYPLLTRQKNQGLLNYRWEESPQGPPRKYYSITDKGREMLSELDVTWKEIVDSI
                     EIIRNTPNQ"
     gene            1047..2582
                     /locus_tag="PLI37_06315"
     CDS             1047..2582
                     /locus_tag="PLI37_06315"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOQ58657.1"
                     /translation="MKNVIKVSVGRWAFTLNEGAYVLLENFLDRLKTYYEKEADTDKR
                     VQDIENRIGEYLSDCVKSIDQVVDEKQIQQAIESLNLPDFGQQAASSGRGAAESDDYG
                     RSQKRLFRSDERKVLGGVLSGLGEYFGIDPVGLRVLYVIFVVIGLFSFRFNMGDHVHF
                     RLFGMGSITTFLLLMYLVMWIVMPLARTPQEKRAMRGGRTSARRVVHDLGTRSGSNYR
                     EERNFGLGSFLLSFIRVFAGVVFIVVGASGLLAFPMLLYFWDVPANWSGYFELLTLRP
                     GLPWFKVWTLIAVFVPLLLFLYEGIVLLFKLRVRKFNLGAVFLVVWILSLFILAFSGL
                     LASRSFWGEGKYQSTVALDLPSDTLYLEIDAEISSPEQFVCAYEDGFFVYCGKNSSGN
                     ECLYVIPQVRLSSGDTPKMTQELSLRAKNNFIAQMKAEDGVDFVKQEGNKIKVTPLYF
                     DQENPWTFEQMSLHLILPENAVLYVQGEKGKWQELKHNRGSFYPLVGYTGDIEGFTIN
                     NLF"
     gene            complement(2692..3270)
                     /locus_tag="PLI37_06320"
     CDS             complement(2692..3270)
                     /locus_tag="PLI37_06320"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOQ58658.1"
                     /translation="MKRTQKHRLAIFASGSGSNAENIARYFAGRADVEVVLFLSNKKN
                     AYVLERGKKLRIASHAFEKTDMEGGGILQLLQDNQVSFIVLAGYLQKIPAEMIQAYPD
                     KIVNIHPALLPKFGGKGMYGERVHEAVIQAGEKESGITIHYVNEHYDQGNIIFQAGCP
                     VLASDTPSELARRVHELEYLHYPRIIDKLLKE"
     gene            3422..3661
                     /locus_tag="PLI37_06325"
     CDS             3422..3661
                     /locus_tag="PLI37_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOQ58659.1"
                     /translation="MSETADKVKSIIAEKLGIELSQVTPTANFTNDLGADSLDTVELI
                     MSFESEFGISIPDEEAEKISTVSDAIDYVEKTLKK"
     gene            3730..4995
                     /gene="fabF"
                     /locus_tag="PLI37_06330"
     CDS             3730..4995
                     /gene="fabF"
                     /locus_tag="PLI37_06330"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOQ58660.1"
                     /translation="MELKRVVVTGLGAITPLGNSVSAYWTGLLNGVSGSGPISRFDTT
                     HFKTTFACEVKNYNSEDYFDRKEVRVYDLFAQFAIISAKEAISDARLIPEQEDLDRIG
                     VIWGAGIGGIQSFENEVANFAVGDGKPRFSPFFVPRMIADLSAGHISIMYGFRGPNYC
                     TVSACASSNNAFFDAFNYIRLGKANVIVCGGSEAAITPCGVGGFNAMRAISTRNEEYA
                     TASRPFSKSRDGFVLGEGGGCLIFEELEHALARKAPIYVEVAGCGLSADAHHITAPHP
                     HGLGATKVMENALEDAGMKPEDIDYINVHGTSTPLGDIAEVKAIKKIFGEHAYRLNVS
                     STKSMTGHLLGAAGAIESVACVKAIQENIIPPTINFTEGDEDPEIDYGINFTFGKACK
                     REVRAALSNTFGFGGHNASIIFKKWNPGV"
     gene            4977..5759
                     /gene="rnc"
                     /locus_tag="PLI37_06335"
     CDS             4977..5759
                     /gene="rnc"
                     /locus_tag="PLI37_06335"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOQ58661.1"
                     /translation="MEPGRITFPFFFSKVFYKKKNREWYPVLRKILGFYPRNLLLYEL
                     AFRHKSASIRGRDGVLLNNERLEFLGDAVLNVLVTDLLFNHFESEGEGFLTNTRSKIV
                     QRETLNNIALELGLDKWVLKAEYALSPRQSIFGNTLEALIGAVYLDRGYRGCKRFLKK
                     KIIQRFIDLEHVANQEINFKSKIIEWCQHQKVKLSFEHIELPAEEESPLNFEARLFLN
                     GIYVACGKGCTKKQAQQKAARDAMTIVESRQALYADIMAAGV"
     gene            5858..6934
                     /locus_tag="PLI37_06340"
     CDS             5858..6934
                     /locus_tag="PLI37_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOQ58662.1"
                     /translation="MSDFFTDIAQLSFKTRLEGLKDYAFESYNIQNEQMFNLLNEAQA
                     CFYGRKYNFRGIFSYQDFRERLPILQNSDIQPYLRKMIAGKPNILWPGVCKHFVEVPQ
                     SGGQKSGNDKYYLPVSDQMLSENFFQGIDDCYALYLRDYPESRLFSAFSVWLGADSKE
                     ENTGNITAVLRKNMPFIMGLLTFPNELPDESADDQLTEKILKKCQQDKISNFHGTPSL
                     FKSFLDEASAFFGNKSIREICPSAEVFFHRGIPFPTQMEQGVEGLNYQASYCTAEGFF
                     GIQERPGEAALLLMIDNSIFYEFIPVDAALEPKNAVPLEEVVLDKDYRMIITTCSGLW
                     RYCSGGPAVRFVSEKPYKFILCNN"
BASE COUNT         1884 a         1435 c         1657 g         1974 t
ORIGIN      
        1 aaccggaggc tttttaattg tgaaataact taaaatatta gtactatgta ttgcatagta
       61 ctatacgctg cattatattt gcccccgaat gccggattct gatgttagaa ccggactaaa
      121 acaaataata aaactcatga aaaaggttgt ttcaaaaggg attgggggct attctttcca
      181 gattgaagag gatgcctgcc gcattttgca ggattacatt gaacaattta ggagcaagct
      241 gggagaggcc gaagacgtct ccgaaatcat ggacgatatc gaggcccgca ttgccgaaat
      301 tctccgggag gaaataactt ctcccaatca ggtcgtgggc gaaggcctga tacgacaggt
      361 agtggcacgc atgggcatgc ccgacggcag caacgatttt accgaatatc acgtgcccta
      421 tgcttcaaac aaaagagctg cacgcaggct ttttcgcaat cccgacgacc gtaagatagg
      481 cggaatttgc ggaggattgg gcgcctattt gggagtggac tccaccctgt tgaggatttt
      541 gtttttgatt ggcttctttt tgggactctt gtcgttttgg gtttacctta ttttgtggat
      601 ctgtgtgccg gacgcaagga cacctgcaca aaaactgcag atgcagggac ttccggtaac
      661 ggccgaaaat ttattgcgtt attcagctaa acaatcagag aaatgaaccg ggaaataaat
      721 acggataatg taaaggctca gatgagaaag ggggttctcg aatacagtat tttatccatc
      781 ttgtccaggg gggatgaata cgcttcttcc ctgatcaaaa ttcttaaaga cgcccaaatg
      841 atagtggtgg agggcacatt ataccctctg ctaaccagac aaaagaacca gggtttactc
      901 aattaccgtt gggaggaatc tcctcagggg cctccccgta agtattacag cattacggat
      961 aagggcaggg aaatgctctc ggagctcgat gtgacctgga aggaaattgt tgacagtata
     1021 gaaataattc gtaatacccc aaatcaatga aaaatgtaat taaagtgagc gtaggtcgct
     1081 gggccttcac cttgaacgag ggggcctatg tcttgctgga aaatttttta gacaggctca
     1141 agacttatta tgaaaaagaa gccgatacgg acaaacgcgt tcaggacata gaaaaccgta
     1201 tcggagagta cttatcggac tgtgttaaat ccatcgatca agtggtggac gagaaacaaa
     1261 tacagcaagc catcgaatcg ctgaatttac cggatttcgg gcagcaagct gcttcgtcag
     1321 gccgtggtgc agcggagagc gatgattacg gcaggagtca aaaacgtttg tttcgcagcg
     1381 atgaacgtaa ggtgctgggc ggagtgctca gcgggctggg cgaatatttt ggtatagacc
     1441 cggttggctt acgtgtcctg tatgtgattt ttgttgtgat cggactgttc agtttccgtt
     1501 ttaatatggg cgatcatgtc catttccgcc tgtttggaat gggaagtatt actacttttc
     1561 ttcttttgat gtatttggtt atgtggatcg taatgccctt agcacgaact ccccaggaga
     1621 aaagagccat gaggggagga cgaacttcgg cccgcagggt tgttcatgat ttaggaaccc
     1681 ggtccggcag caattaccgc gaggagagga atttcggtct gggcagtttt ttgctcagct
     1741 ttatccgtgt ttttgcaggt gttgttttta ttgtggttgg agcctccggc ttgctggctt
     1801 ttcccatgtt gctgtatttc tgggatgtac ccgccaattg gtctggttat tttgaattat
     1861 tgactttgcg tccgggtttg ccctggttta aggtatggac attaattgcc gtttttgtgc
     1921 ctttactgtt gtttttgtac gaagggatag ttctgttgtt taaactgcgg gtgcgtaaat
     1981 ttaacttggg agctgttttt cttgtcgttt ggatattatc tctttttatc cttgctttca
     2041 gcggtttact tgccagccgt tctttttggg gtgagggaaa atatcagagc acagtagccc
     2101 tcgacttgcc ttcagatacc ttgtacctcg agatagatgc cgaaatttct tcgcctgaac
     2161 aatttgtatg tgcctacgaa gatgggttct ttgtttattg cggcaaaaac agctctggta
     2221 acgaatgtct ctatgttatt cctcaagttc gcctaagtag tggagatacg cccaaaatga
     2281 cccaagaact gagtttaagg gccaaaaaca actttattgc ccaaatgaag gccgaagacg
     2341 gcgttgattt tgtaaaacag gagggaaaca aaattaaagt caccccttta tattttgacc
     2401 aagaaaatcc ctggaccttc gaacaaatga gcctgcattt gattttaccc gaaaatgctg
     2461 tcctgtatgt gcagggggaa aagggtaaat ggcaggaatt aaagcataac agaggttcgt
     2521 tctatccttt ggtgggctat accggcgata ttgaaggctt tacgattaat aatttatttt
     2581 agcggcatca acaggatcgt tttatcaatc ctccggaact tagttttgct tccggaggat
     2641 tttttgaata atgcccattc gagggatagc aattatacaa ggatgcaata attattcttt
     2701 caaaagtttg tctataatcc tggggtagtg caggtattcc agttcgtgta cgcgccgcgc
     2761 aagctccgaa ggcgtatccg aagccaatac cggacagccg gcctgaaaaa taatgttccc
     2821 ctggtcgtag tgctcgttta catagtggat ggtaatgccg gattcctttt cgccggcctg
     2881 tataacagct tcgtgtaccc gttcgccata catacctttg cccccgaatt tgggcaataa
     2941 tgcggggtgg atgtttacta ttttgtcggg gtaagcctga atcatttcag ccggaatttt
     3001 ttgaagataa ccggccagaa caataaaact tacttggtta tcctgtagca gttgcaggat
     3061 gcctcccccc tccatgtctg ttttctcaaa agcatgggag gcaatgcgga gtttttttcc
     3121 cctttcgagc acgtaggcat tttttttgtt ggaaaggaat aaaacaactt ccacatctgc
     3181 tcttccggca aaataacggg caatattttc tgcgttagag ccggagccgg aggcaaaaat
     3241 ggcgagacga tgcttttggg ttcttttcat aattgacaga tatccaggga tttattaaaa
     3301 aacgaaaagc gtttttagaa taattagagc acaaaattag cgtttgtttg aaatatttca
     3361 ttcctttgtc gtctcaattt ttttgagaca aacttattta ttcactaaaa aatgaacgat
     3421 tatgtctgaa acagcagata aagttaaatc gattattgca gaaaaattgg gcattgaact
     3481 ttctcaagtt actcctactg caaacttcac taatgatctt ggtgctgact ctctcgacac
     3541 cgttgagttg attatgagtt tcgaatcgga attcggaatt agtattcctg atgaagaggc
     3601 tgaaaagatc tcaacagtca gtgatgcgat cgattacgtt gaaaaaactc tgaaaaaata
     3661 attccgtaca ggagttcccg gtacataaga ggaaaaccgg acgcgggact tcttgaagat
     3721 tcctagaaaa tggaattaaa aagagttgtc gtaacaggcc ttggagctat tacccctttg
     3781 ggtaattccg tatcagcata ttggacgggt ttacttaacg gagtaagtgg ttccgggcct
     3841 atttcacgtt ttgacaccac gcatttcaaa acaacatttg cctgcgaggt aaagaattat
     3901 aattcagaag attatttcga tcgcaaggag gttcgtgtat acgacctttt tgcgcaattt
     3961 gctattattt cggcaaaaga agctatttcc gatgctcgcc tgatccccga gcaggaagat
     4021 ttggacagga ttggagttat ttggggtgcc ggtataggag gtattcaaag ttttgaaaac
     4081 gaagtggcca attttgccgt aggagacggc aaacccagat tcagtccgtt ttttgttccc
     4141 cggatgatag ccgatctatc ggcaggccac atttcgatca tgtatggctt ccggggtccg
     4201 aattattgta ccgtatcggc ttgtgcttcg tcgaataacg ctttttttga cgccttcaat
     4261 tacatacgtc tcggcaaagc caacgttatt gtttgcggag gttccgaagc agccattact
     4321 ccttgtggag taggcggatt caacgccatg cgtgccattt ctacccgcaa cgaagagtac
     4381 gccaccgcca gccgtccttt cagcaaaagc cgcgatggtt ttgtgctggg cgagggggga
     4441 ggctgtctga ttttcgaaga gctcgaacac gcgctggctc gtaaggctcc tatttacgtc
     4501 gaggtggccg gttgtggatt atcggccgac gctcaccaca tcacagcacc ccatccccac
     4561 ggtctgggag ccaccaaagt aatggaaaat gcgctggagg acgccggaat gaaacccgaa
     4621 gatattgatt acatcaatgt acacggcacc tcgacccctc tgggcgatat tgccgaagta
     4681 aaggccatca aaaaaatatt tggcgaacac gcgtatagat tgaacgtcag ttccaccaaa
     4741 tcgatgaccg gacacctgct gggtgcagcc ggcgcgatag aatctgttgc ctgcgtcaag
     4801 gccatacaag aaaacatcat tcctcccacc atcaatttta ccgaaggaga cgaagatccc
     4861 gaaatcgatt acggtattaa ttttactttt ggaaaagcct gtaaacgtga ggttcgtgct
     4921 gctttgtcca ataccttcgg atttggtgga cataatgcga gcattatttt caaaaagtgg
     4981 aacccgggcg tataactttt ccgttttttt ttagcaaggt tttttataaa aagaaaaaca
     5041 gggaatggta tcccgttctt agaaagattc tcggatttta tccccgtaat ctgcttttgt
     5101 atgaattggc ttttcgacac aaatcggctt cgataagagg ccgcgacggc gttttactca
     5161 ataacgaacg tcttgaattt ctcggagatg ccgtattgaa cgtgttggta accgatttgc
     5221 tcttcaatca ttttgaatcc gagggagaag gatttctcac caacacccgt tcaaaaatcg
     5281 tacagcggga aaccttaaac aacatagcac tggaactggg tttggacaaa tgggtactca
     5341 aagccgaata tgccttatct cccagacaat ccatttttgg gaacacgctc gaagcgctta
     5401 taggggccgt ctatttagat agaggttacc gcggctgcaa gcgttttctc aaaaagaaaa
     5461 tcatacagcg gtttattgat cttgaacacg ttgctaatca agaaattaac ttcaaatcga
     5521 agattattga gtggtgtcag catcaaaaag ttaaattgag ttttgaacac attgagctgc
     5581 cagccgagga agaatcaccg ctcaatttcg aagcccgtct ttttctgaac ggcatttacg
     5641 tagcctgtgg caagggatgc accaaaaaac aggcccaaca aaaagcagcc cgcgatgcga
     5701 tgactatcgt cgaaagccgg caagctttgt atgccgacat tatggccgcc ggcgtatagc
     5761 gaaaacacag gaattttatt tttgaaattt tattcccggc ttgaaagctg tggaaaaatg
     5821 aattactttt gaggctgata ttcaagcccc ctgtgtcatg agtgattttt ttacagacat
     5881 tgcccagttg tcattcaaaa ccagactgga aggattgaaa gattacgcat ttgaatctta
     5941 caatatccag aatgagcaga tgttcaactt actgaacgag gctcaggctt gtttttatgg
     6001 gcgaaaatac aattttagag gtatttttag ttatcaggat tttcgcgaaa ggcttcccat
     6061 tctgcaaaac tccgatatac aaccttattt gcgcaaaatg attgccggca agcccaacat
     6121 actgtggccg ggcgtttgca aacattttgt ggaagttcct caaagcggag gtcaaaaatc
     6181 cggaaacgat aaatactact tgcctgtcag cgatcagatg ctgagcgaaa atttctttca
     6241 gggtattgac gattgctatg ctctgtattt gagagactat cccgagagta ggcttttctc
     6301 agccttttcg gtgtggttgg gggcagacag caaagaagaa aacacaggaa atatcacggc
     6361 tgttttacgt aagaatatgc cctttataat gggcttactc acttttccca atgagttgcc
     6421 cgatgagtcg gccgacgatc aactgacaga gaaaatttta aaaaaatgtc agcaggataa
     6481 aatttccaat tttcatggaa caccttcttt gtttaagagc tttttggacg aagcttccgc
     6541 ttttttcggc aataagagca ttcgggaaat ctgcccctcg gccgaggtgt tttttcacag
     6601 ggggattccc ttccccactc aaatggaaca gggcgtagaa ggattgaatt atcaggcttc
     6661 gtattgcaca gccgaaggtt tttttggcat tcaggaacgg cccggcgaag ccgccctttt
     6721 attgatgata gacaacagca ttttttacga atttataccg gtcgacgctg cgcttgaacc
     6781 caaaaatgca gttcccttgg aagaagtcgt tctggacaaa gactatagaa tgattattac
     6841 cacatgctcc ggcttgtgga gatattgctc cggtggcccg gctgttcgct ttgtctcgga
     6901 gaagccctac aaatttatat tatgcaacaa ctgagtattt acaatacatt
//
LOCUS       DAOSTD010000088         6906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_10158_length_6906_cov_13.003503, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000088 DAOSTD010000000
VERSION     DAOSTD010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10158_length_6906_cov_13.003503"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLI37_06345"
     CDS             complement(<1..521)
                     /locus_tag="PLI37_06345"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOQ58663.1"
                     /translation="MIKCLKTIGILLLLTGIAVLPVKAQDGLVTVTGKVLSQADQKGL
                     PGISVTVQNLTSVMTADDGGFSLSLPSADAELSISGPGYQTKKVALRGRTQLIVKLHA
                     DNFTNSVYKEVLMPLGTVNNSHLSAAAVFLDQDKSTEVAIMPEQLLQAKVSGLNAMFR
                     SGMEGSGANLFVRG"
     gene            complement(544..>1198)
                     /locus_tag="PLI37_06350"
     CDS             complement(544..>1198)
                     /locus_tag="PLI37_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58664.1"
                     /translation="TVLDGVDTTLSKTVNYYYYSATASGSYYDKSINFYLKYTPTLYS
                     TKYRLYWKTYDDFGRHFAGDTLDVLFNPEDPNQACPSVYRVTQKLYISLPGRPELKRN
                     NGIIENLSLQSGAKVMVGITPDTTDASGRGHTIGVNAGVETEMPLRWYKAAGDYEALS
                     TPLAAANTRDYLNCTSMGTACFYVTNSPYTSASNSTSNGLIFLDYIRLVPEMIDEND"
     assembly_gap    1199..1298
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1299..2147)
                     /locus_tag="PLI37_06355"
     CDS             complement(<1299..2147)
                     /locus_tag="PLI37_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOQ58665.1"
                     /translation="MKYLSVKSLGLILLSTVLLGCADQWAEHIRPVDKNLETSLFQAI
                     KAEQELSLFQAFLQETGYDAVLESTENYTVFAPLNTALSAYQSASVEVKKNLVKNHIA
                     ALSYNTADLNSMEYLMMLSGKRLRLDELSLNAKETVCSNGILRTSNSISLPKANLYEL
                     ISAYKDSLEIARAIFLAGDSIMDPVKSIQTGVDPHSGRPIYDTAYLYYNPYLESLSIH
                     QEDSSYCFVLLNNSNFADLKAKYAKYMQQWDESIRAVNDSLTDLVVTRELINDLICPT
                     EGFEESA"
     gene            complement(2169..3968)
                     /locus_tag="PLI37_06360"
     CDS             complement(2169..3968)
                     /locus_tag="PLI37_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOQ58666.1"
                     /translation="MRTDQFLNKKPVFNGILIGLVVMLALFSSCKTNLDKGLYQTTAE
                     LMIDEYLESEEELSLFLNIIDIADLRGMVHAYGTYTCFAPTNQAVSEYLEDKNLDLAT
                     LSQAEAAYMVKYHFISDTLSTADFVESRLSAANMNNQFLTTKTVMDEEGNIQIIVNRK
                     AKILEANIRLGNGLVHVVDHFLSLPTQSVEESLEAMPDSVFSTMKFFVSKLKTDYNIK
                     IDDFLKDTAYITLLVQTNAAFEKAEIADIAGLLARLKENDLKSEFTDRQLLSNWLGYH
                     CLPGRIYIADVLLSTTLNTSVKGQPITITLVDEHLYLNRFKTERIDEDGIFMDRQGVY
                     VDYNCSNGVVQTIDDMLEIIQRSAYRVYWDMGDQPEIKALKGYRKAGTKVTYLPGELS
                     MMTWGGSNNPTVTYSAWGLPDINGAYDQKSQYVYNDYLTFRVCTRVMQWLEIKTPVLV
                     AGKYHVWVCWRRMNPCKFRTTFKQEGEEDQVFPAVVDLQDYMPVELDDDQLEAKGWKQ
                     YNAKFRISVFNSKNVATIDVKTTGSHILRFDALVGSKDVGNNWDMIQFIPINDDQVWP
                     MIAMDGTLVAKGTPDCQIFPEDGSNCPVEEEEE"
     gene            complement(3971..5563)
                     /locus_tag="PLI37_06365"
     CDS             complement(3971..5563)
                     /locus_tag="PLI37_06365"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ58667.1"
                     /translation="MKKSNKILALLLPLLLMSSVSCSDWLNLLPEDSVPKQSYWKSKE
                     DVVSALKGIYSSMLSSDLSQKIWLWGEIRADMITDGRSVNASLNLVRQGEISADNSLC
                     NWSSFYTTINNCNILLRFAPEAYEKDESFTQRELESYLAQAVAVRALMYFYLVRTFGD
                     VPFSLEAYVDDSQYLPLAKSPGSVILDSLVLQLEGLRVNNSIPYQYGSAAENKGQMTR
                     YAVDALLADIYLWKEDYASCVVVCDEILNSGQFALIPVPKAEAVTDQGDTVLHPSPAG
                     IYDLFQSLYIDGNSVESIFELPFDRDITNPFYQLLVYQNRSLMPNALYINSDIFVPSA
                     KGDPEYFDIRASINSKGGYVWKMAGVTYDGNDVFTVENMTNNLIIYRLAQVYLMKAEA
                     LTQLAGQDQEKLEEAYGLLRQLRERSCAVETTELERDGSGNIDRDLLELFVMEESARE
                     LMFEGKRWFDVLRNAKRNNYSKLNYLMSIVPNSTTPDKVYSLQVKYKNFNSHYLPLPQ
                     ADIESNPLLVQNPFYDTKINKP"
     gene            complement(5578..>6906)
                     /locus_tag="PLI37_06370"
     CDS             complement(5578..>6906)
                     /locus_tag="PLI37_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOQ58668.1"
                     /translation="ASLRYRISGEPFMRKQSERWLDELSLRASYGVNGAQPRGNYASV
                     ATYDVFNFEYLGASGTYQRNLELVDLRWENTTQANIGLNFASFKNRLGFDFEWYHKET
                     RDLFMYNLKIPTSTGFSSVNTNVGRMDNNGWEFQCFATPVKTKDWTLTLNLNFARNDN
                     RLREISDLYPQESGISTSNASYISQLIVDQPLGSFYGYLYDGVYLNEDQTIARDKKGD
                     KIYTYNDKGEKEPVYMRFSYPSIDYQFQPGDARYVDVNHDGNIDYQDIVWLGNAMALF
                     SGGFGGKLSWKQFTVDAFFNFRYGNDVINFAKLNMENMYAYDNQSLSVLRRWRHVYED
                     ESEAPDDLLPRALYRKGYNYLASDRFVEDGSFVRFKSLTFRYNMSRDQLKNTFLQRAS
                     FFITLQNLYVWTAYTGMDPEVNISNPLSAGYDYASTPRPKDYMIGFSLTF"
BASE COUNT         1795 a         1667 c         1541 g         1803 t
ORIGIN      
        1 ccccggacaa aaagattcgc tcccgagcct tccatacccg atctgaacat ggcgtttaaa
       61 cccgatacct tagcctgtaa aagctgttcg ggcataatag ccacctcggt gcttttgtcc
      121 tgatccaaaa aaacggctgc tgcgctcagg tggctgttat ttaccgtccc caagggcatc
      181 agcacctctt tgtagacgga attggtgaaa ttatccgcat gtaatttcac tatcaattga
      241 gttctgcccc tcagcgctac tttcttagtc tgataacccg ggccggagat actaagttct
      301 gcgtcggccg aaggcaggct caggctaaaa ccgccatcgt ctgccgtcat aacagaggtg
      361 aggttctgca cagttacgct gataccgggt aagccttttt gatcggcttg agacaacacc
      421 ttaccggtaa cagtcacaag cccgtcctga gccttgaccg gtaaaacggc aatccctgtc
      481 aacagcagga gtatccctat ggtttttaag cattttatca tggttcttga gcttgagtat
      541 tcgctaatca ttttcatcga tcatttcagg taccaaacgg atataatcca aaaagatcaa
      601 accgttggaa gtgctattag aagcactggt atagggagaa ttggtaacat agaaacaggc
      661 tgttcccatc gaagtgcaat tcaggtaatc gcgggtattg gcagcggcca gaggagtgga
      721 cagcgcttcg taatctccgg cggctttgta ccaacgcaga ggcatttcgg tttcgactcc
      781 ggcatttacg cctatggtgt gaccacgtcc cgacgcatcg gtcgtatcgg gggtaatgcc
      841 caccattacc ttggcccccg actgaagcga caagttttca atgataccgt tgttccgctt
      901 caattccggc ctgcccggca acgaaatgta aagcttttgg gtaacccggt acaccgaggg
      961 gcaagcctga ttgggatctt cgggattaaa aagcacatcc agagtatcac cggcaaaatg
     1021 acgtccaaaa tcgtcgtaag ttttccaata aagccggtat ttagtcgaat agagcgtagg
     1081 tgtgtacttg aggtaaaaat tgatggactt atcgtaataa gagcccgaag ccgtggcgct
     1141 gtaataataa tagttgaccg ttttagacaa ggtggtgtcc accccgtcga gcacagtgnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg cgctttcttc aaaaccttcg
     1321 gtggggcaaa tcaaatcgtt gataagttcg cgtgtcacca ccaaatccgt caaactgtcg
     1381 ttgaccgctc tgatactttc gtcccattgc tgcatgtatt tggcgtattt ggccttgaga
     1441 tcggcaaaat tgctgttgtt gagcaataca aaacaatacg aagaatcctc ctgatggatc
     1501 gataaacttt ccaggtaagg attgtaatac aggtaagccg tatcgtaaat aggtctaccg
     1561 ctgtggggat ctactccggt ttgtatgctc ttaacgggat ccataatgga atcgccggcc
     1621 agaaaaatgg cccgggcaat ctccaaactg tccttatagg ctgaaatcag ttcgtaaaga
     1681 ttggccttgg gcaagctgat gctattgctg gtgcggagaa taccgttgct gcatacggtc
     1741 tcctttgcat taagcgacag ttcgtccagc ctgaggcgtt tgccactcag catcattaaa
     1801 tactccatag aattcaagtc tgccgtgttg taactcaatg cggcaatatg gtttttaacc
     1861 aaattcttct ttacttccac cgaggccgat tggtaagcag ataaggctgt attgagcggt
     1921 gcaaaaaccg tgtagttttc cgttgattcc aacaccgcat cgtaacctgt ttcctgcagg
     1981 aaagcctgaa acagactaag ctcctgttca gcctttatgg cctggaacaa cgaagtttcc
     2041 agatttttat ccaccgggcg gatgtgttca gcccactgat ctgcacaacc cagcagcacg
     2101 gtgctcaaca atataaggcc taaacttttt acagaaaggt atttcattgt ttttctatta
     2161 attattagct actcttcttc ctcttcgacc ggacaattgc tgccgtcttc gggaaagatc
     2221 tgacaatcgg gggtgccttt tgcaaccagg gtaccgtcca tggcaatcat gggccatacc
     2281 tgatcgtcgt tgatcggaat aaactgtatc atatcccagt tgttacccac gtctttggag
     2341 cctaccagag cgtcaaaacg caaaatatgc gatccggtcg tttttacgtc tatggtggcc
     2401 acatttttac tattgaagac gctgattctg aatttagcgt tgtattgctt ccagcctttg
     2461 gcctccaact ggtcgtcgtc cagttccacc ggcatataat cctgcaggtc tacaacggcc
     2521 ggaaacactt gatcttcttc gccctcctgt ttaaaagtgg ttctgaactt acagggattc
     2581 atacgcctcc agcaaaccca aacgtgatat ttgccggcca ccagcaccgg agtcttgatt
     2641 tccagccatt gcatcaccct ggtacacacc ctgaaagtaa gataatcgtt gtacacatac
     2701 tgcgattttt gatcgtaggc tccgttgatg tcgggcaaac cccaggcgga ataggtgacc
     2761 gtgggattat tgcttccgcc ccaggtcatc atagacaatt ctcccggcag atacgtcact
     2821 ttggtgccgg ctttacggta acctttgagt gcctttatct cgggctgatc tcccatatcc
     2881 cagtacacac gataggccga ccgttgtata atttcgagca tgtcatcgat ggtttgcacc
     2941 actccgttcg aacaattgta atccacataa acaccctgtc tgtccataaa aataccgtct
     3001 tcgtcgattc tttccgtttt gaagcgattc aaatagaggt gttcatcgac taaggtgatg
     3061 gtaataggct gacctttgac ggaagtattc agcgtggtag acaacaatac gtcggcaatg
     3121 taaatcctgc ccggcagaca atgataaccc agccagttgc tcagcaattg ccggtcggta
     3181 aattccgatt tcaggtcgtt ttccttgaga cgagccagca aaccggcaat atcggcaatt
     3241 tcagcctttt cgaaagcggc attggtctga accagaagcg taatgtaggc ggtatctttc
     3301 aggaaatcgt ctattttgat gttatagtct gtctttaact tgcttacaaa aaatttcatg
     3361 gtagaaaaaa ccgagtcggg catagcctcc aggctttcct ccaccgactg tgtcggcagc
     3421 gacaaaaagt ggtccaccac atgcaccagg ccgttcccca gcctgatatt ggcttccaat
     3481 attttagcct tgcgattgac aatgatttga atattgccct cttcgtccat cactgttttg
     3541 gtcgtcagga actgattgtt catattggct gcgctcagac ggctttcgac aaaatcggcc
     3601 gtcgaaagag tgtcgcttat gaaatgatat tttaccatat aggcagcctc cgcctggctc
     3661 agagtggcca gatcgagatt tttatcttcc agatactcgc tgacggcctg attggtcgga
     3721 gcaaagcagg tataggtgcc gtaggcatgc accattcccc tgagatcagc aatatctata
     3781 atattcagaa acagcgacaa ttcctcttcg ctttccaggt attcatctat catcaattcg
     3841 gccgtagtct gatacaaacc tttgtccaga ttggttttac aagaactgaa caaagcaagc
     3901 atcacaacca ggccaatcag aattccgttg aataccggct ttttatttaa aaattgatct
     3961 gttctcatgt ttatggctta tttattttgg tatcgtaaaa aggattctgc accaacaaag
     4021 gatttgactc aatatccgct tgaggcagag gcaaataatg gctgttgaaa tttttgtatt
     4081 tcacctgaag actatacacc ttgtcgggtg tggtgctgtt gggcactata gacataaggt
     4141 aattcagttt gctgtaattg tttcttttgg catttctcag cacgtcaaac cagcgtttgc
     4201 cttcgaacat caattcacgg gcagactctt ccatgacaaa aagttccagc aggtcacgat
     4261 caatattgcc cgaaccatct ctttcaagct cggtggtttc gaccgcacaa gaacgttcac
     4321 gcaactgacg gagcagcccg taagcttctt cgagcttttc ctgatcttgt ccggccagtt
     4381 gagtcaaggc ctctgctttc atcagataaa cctgagccag gcggtaaata atcaggttat
     4441 tggtcatatt ctccaccgta aacacatcgt taccgtcgta agttacaccg gccatcttcc
     4501 agacataacc ccctttggaa ttgatggaag ccctgatatc gaagtattcg ggatctcctt
     4561 tggcggaagg aacaaaaata tcgctattaa tatacagagc attgggcatc agtgagcgat
     4621 tttgataaac cagaagttgg tagaaaggat tggtaatatc ccggtcaaag ggcaattcaa
     4681 agatgctttc cacactgttg ccgtcgatat aaaggctttg gaacaaatcg taaataccgg
     4741 ccggcgaagg atgaagaacg gtatcgccct ggtcggttac ggcttccgct ttgggtaccg
     4801 gtatcaaggc gaattgtccg ctgttcaaaa tttcgtcgca aacaacaaca caagaagcgt
     4861 agtcttcttt ccaaagataa atgtcggcca aaagggcatc gacggcatag cgagtcattt
     4921 gtcccttgtt ttcggctgcc gaaccgtatt ggtagggaat gctgttgttg actctaagac
     4981 cctccagttg caacactaag ctgtcgagta tcacactgcc gggagacttt gccagaggca
     5041 gatactgaga atcgtccaca taggcctcca gcgaaaaagg aacgtcaccg aaagtgcgaa
     5101 ctaagtaaaa atacatcaga gcgcgcacag caactgcctg agccaaataa gattccaact
     5161 cccgttgggt aaaggactca tccttttcgt aagcctcggg ggcaaatctc agcagaatgt
     5221 tgcaattatt tatggtcgta taaaaagatg accagttaca caggctgttg tctgcagaga
     5281 tttcaccctg cctgaccaaa ttcagcgaag cattaacgga acggccatcc gtaatcatat
     5341 ctgccctgat ttcgccccaa agccagattt tctgactcag atcagacgac aacatagagc
     5401 tgtaaatacc cttcaaggcc gaaaccacat cttctttact tttccagtaa gactgtttgg
     5461 gcacactatc ctcgggcagc aaattcaacc aatcgctgca cgaaacagag ctcatcagga
     5521 gcaaaggcag taacaaagcc agtattttgt ttgatttttt cataacttcc gttcgcatca
     5581 aaaggttagt gaaaagccta tcatatagtc tttgggccgg ggcgtgctgg catagtcgta
     5641 accggcagac aagggattac tgatattgac ttcggggtcc atgccggtat aagcggtcca
     5701 tacatagaga ttctgcaagg ttataaagaa cgaagcccgt tgcaggaagg tgttcttcaa
     5761 ctgatctctg gacatattat accggaaggt aagcgattta aaacgcacaa aagaaccgtc
     5821 ttcgacaaac ctgtccgaag ccaggtaatt ataacccttt ctgtacaaag ctctgggaag
     5881 caggtcgtcg ggcgcctccg actcgtcttc gtacacatgc ctccagcgcc tcagcaccga
     5941 caaactttga ttgtcgtaag catacatatt ctccatattc aacttggcaa agttgatcac
     6001 gtcgttgccg tagcggaaat tgaagaaagc gtccacagta aattgcttcc agctgagttt
     6061 cccaccgaag ccgcccgaaa acagagccat ggcattaccc agccacacaa tatcctgata
     6121 atctatatta ccatcgtggt tcacgtccac ataccgggca tcgccgggct ggaactgata
     6181 atcgatggag gggtagctaa agcgcatgta aaccggctct ttctcaccct tatcgttata
     6241 agtgtaaatt ttgtcgcctt ttttgtcgcg ggcaatggtt tgatcttcgt tcaggtacac
     6301 accgtcgtac aaatagccgt aaaaagagcc caggggctga tccactataa gttgcgatat
     6361 gtaactggcg ttagaagtac tgatacccga ttcctgggga tacaaatccg aaatctcgcg
     6421 cagacggttg tcgtttcggg caaaattcag gttcagcgtt agagtccaat ctttggtttt
     6481 gaccggtgtg gcgaaacact ggaactccca gccgttgttg tccatacggc ccacattggt
     6541 gttgaccgaa gaaaagcctg tagaggtagg aatcttcaga ttgtacataa acaagtcgcg
     6601 ggtttccttg tgataccatt caaaatcgaa gcccaggcga ttcttgaacg aagcgaaatt
     6661 cagaccgatg ttggcctggg ttgtgttctc ccaacgcaaa tcgaccagtt ccaggtttcg
     6721 ctgataagta ccggaagcac ccaagtactc aaaattaaat acatcgtaag tggccaccga
     6781 ggcataatta cccctgggct gagctccgtt cacaccgtaa ctggcacgca aactgagttc
     6841 gtcaagccaa cgttcacttt gtttcctcat aaaaggctct cccgatatgc ggtaacgcaa
     6901 cgaagc
//
LOCUS       DAOSTD010000089         6703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_10541_length_6703_cov_13.908093, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000089 DAOSTD010000000
VERSION     DAOSTD010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10541_length_6703_cov_13.908093"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PLI37_06375"
     CDS             complement(<1..453)
                     /locus_tag="PLI37_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ58669.1"
                     /translation="MKISFLKGLFFTVTISICFASCERMMETESSLLSYPEEHELNTS
                     ADTVYSVIGIMNKIQTLADRSVLLGELRADLVDVNENTISSLREIANFEISEDNPYNQ
                     PKDYYAVINNCNYFLQKADTALRKRNIPVFLREFAAVKAFRAWTYLQLV"
     gene            complement(465..>2483)
                     /locus_tag="PLI37_06380"
     CDS             complement(465..>2483)
                     /locus_tag="PLI37_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOQ58670.1"
                     /translation="RNLRDDGVMPPEESNVFSPGFLSLIKSPFLSPYQYDNNKNLSGF
                     LSDADDYLNEVLGSRASLANPLYIFENGEARNKNYFENSLINLAIRPAYSFNKNLSVK
                     GHFSYTLVNTNENYYIPMTGVPKIIIPNVGTAENLRKSLYSSQISLFADLRMLWQKKR
                     GANDTRLMAGGRYMNDDFSGNTQMGYDTGNDKTPNMNANLNFKKVDGAEDVWRSLSYY
                     ANLDYNYAEKYYLHAGLSMETSSRFGREVQGGIRMGGVSWGLFPSAAAAWVLSSEDWF
                     RLRSVDYLKLNLAYDISGNDDIDYYAARTYFGSSVFMDRVTGLTIQNIGNTSIQWEST
                     QKGGAGLEASLFQERVGIQLNAFLAKTDNLLTLKKLASVTGLDYNWSNEGSLTNRGFD
                     LALNFKLVNRNKFNWGMGFSLGHYKNEITALPDDESFITKLYGAEILSEKGRPAGLFY
                     GYQTKGVYASSEEAAADGLYILSKTGSKVYFGAGDVIFVNNRDDSKEINADDKVVIGD
                     PNPDLYGNIFSNIHYGRFSLDLILNYSLGNDAYNYQRSQLESGSKFYNQSLALLNRWT
                     NEDQKTTIPRISYLDEMGNSRFSDRWIEDASYLRLKTVRLSYSLPLNTTFLNGLTLWA
                     SGNNLWTLTRYLGSDPEFSSGNAVLCQGIDRGTLAFGPSVVLGIKLNL"
     assembly_gap    2484..2583
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2584..3796)
                     /locus_tag="PLI37_06385"
     CDS             complement(<2584..3796)
                     /locus_tag="PLI37_06385"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOQ58671.1"
                     /translation="MKNCYAKIKFRGLLVLMLCLSTFTVSAQTLESKTITGTVSEAST
                     GKPLVGARVQAYNNTRFVAMTDGEGRYTLEIPVYVNVLTVTLDGYALRHMALKGQTSG
                     VDLVLYSENFVPVLSPLTNVQNAGSAERFDLSSAISIESEIQSQLGAEVRTVSRSGIP
                     GAGSVMFVGGYNSLNVNAQPLIVLDDVILDQQYGRDLLHEGYYNNILSNLSVNDIESV
                     TVLKSGTSLYGAKGANGVLIIKTKRNKSMVTRIDACASAGVEFIPRLPRMMNAAQYRA
                     YASELLGTTDTKLTEFKFLKEDPDYYYYNQYHNETNWSDYVYREALTQNYSFGVQGGD
                     EVAKYNLSIGYVDAQSTLNYNDFDRLNLRFNSDISISDKLSALFDCSFSNVTRNLRDD
                     GVMPPEESNVFS"
     gene            complement(3805..5427)
                     /locus_tag="PLI37_06390"
     CDS             complement(3805..5427)
                     /locus_tag="PLI37_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOQ58672.1"
                     /translation="MKRSIFNIRYGLILLPILPLVWACSNDWDKHYGADPEVVPAQSL
                     WESIEARPDVLSDFADLLRLCGYDKVLDADQVYTVWAPANGHFNVDSLRTLINQGKKE
                     QVVTEFVKNHIARYSHPVSSLTDKHVLLLSKKTERLRSNEAKTSYLFGQTGIMNKNLL
                     SSNGILHIIDKANPFFFNIYEYLKTEPGLDSLKSFIYSFDELVFDPLLSVPYGVVDGK
                     TIYVDSVVTIENKLLEKIGLINHEDSTYWLLAPSNRAWNEAYDSIRQYFKYDFKVAKA
                     DSLEQVNTKMMMVRDLVFNANLQASPRDSLFSAQYRPLDKRYHVYQRPFDEGNIFSRI
                     TSEIVCSNGTVYTTDDWLYKPEQNFYQEIKVEAERTTGRDYHSSLCDIMDRAIKPEDD
                     INISNRRYIHLLPAGTSRNPWVSFEIPNTLSASYDIYCVFVPYKTYNPTHENVDKPTK
                     LKFSLTYNDEKGNSRTISCKDSTGNNIYITDPSKLDSVLVLSDFKFPVANYGQEKTTV
                     LLKIETSITSKETAFYTREMLIDCICLKPHLK"
     gene            complement(5472..>6703)
                     /locus_tag="PLI37_06395"
     CDS             complement(5472..>6703)
                     /locus_tag="PLI37_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOQ58673.1"
                     /translation="YDYVQPKLVLHYTSHVNRETAIAPKYRKYGYTGFVEPDEVYRQK
                     GINNMNDLIAYAKKVYDEVYPQDANLYDDDFTHPKNPLNRFVAYHFMPRTGYYNKLTT
                     TLCLITSIIDPIEFYETMCPNTMFKISGTTGLYMNRRVDNKFNIRGALIQPPGLSSTD
                     QNAINGTYHYIDDILAYSKEVEEIVFNTRFRFDTGALFPEFMTNNVRHNQDRQNYYFP
                     NGYTEKAELTSETKFIYRYQHENYDCHQGDEINFLGQYDITITLPPVPEGTYEIRLGY
                     TALPTRGISQMYVDGEPTGIPVDLRIGATDPKIGWIADERTDDNGVQNDKDMRNHKAM
                     KAPDSMRRLNGNVYVVFRTESWLFRRILVTKYLTQEPHTLRFRNVIDDLDAEFMFDYI
                     EIVPKSVYDSEEGEDRH"
BASE COUNT         1735 a         1453 c         1525 g         1890 t
ORIGIN      
        1 caccagttga agataggtcc aggcacgaaa ggctttgact gcggcaaatt ctctcaaaaa
       61 aacgggtatg ttgcgtttcc gcagtgccgt atctgctttt tgaagaaaat aattgcagtt
      121 gttgattaca gcgtaatagt ctttaggttg attataagga ttgtcttcgg aaatttcaaa
      181 gttggcaatt tcacgcagac tgcttatggt gttttcgttt acatccacta aatcggctct
      241 gagctctccc agcaacacgc tgcgatctgc cagggtctga attttgttca tgataccaat
      301 aacggaataa accgtgtcgg cagaagtatt aagctcgtgt tcttcgggat aagagagcaa
      361 cgaagattct gtctccatca tgcgttcaca agaggcaaaa cagattgaaa tcgttactgt
      421 aaaaaacagg ccttttaaaa aagatatctt catgattaag agatttagag gttgagtttg
      481 atgcctaaaa ccacactggg accaaaagcc agtgtaccac ggtctatgcc ttggcagagc
      541 accgcattcc cgctcgagaa ctcaggatcg ctgcccaaat agcgggtcag ggtccataaa
      601 ttgttgcccg aagcccaaag agtcagtcca ttcaaaaagg tagtgttcaa aggaagactg
      661 taacttagcc tgactgtttt cagacgcaaa tacgaggcat cctcgatcca tcgatccgaa
      721 aaacggctgt tgcccatttc gtccagatac gaaattctgg gtatcgttgt tttttgatct
      781 tcgttggtcc agcggttcaa taaagccagg ctttgattgt agaacttgct gccgctttcg
      841 agttgagaac gctggtagtt ataggcatcg ttgcccagag aataattcag aataagatcg
      901 aggctgaagc gcccgtaatg gatattgctg aatatatttc cgtaaagatc ggggttagga
      961 tcgccaataa ccaccttatc gtcggcattg atttccttgc tgtcgtcccg gttgttaaca
     1021 aaaatcacat cgccggcacc aaaatatact ttggagccgg ttttgctcag aatatataaa
     1081 ccgtcggcag cggcttcttc ggaactggcg taaacccctt ttgtctgata accgtaaaac
     1141 aaaccggcag ggcgaccttt ttcggaaaga atttcggctc cgtacaactt ggttataaag
     1201 gattcgtcgt cgggtaaagc ggtaatttcg tttttataat gtcccaggct gaaacccatt
     1261 ccccagttga atttattccg gttgaccagc ttgaaattca gcgccagatc aaaacctctg
     1321 ttggtcaaag agccttcgtt gctccaattg taatcaagtc cggtgacact cgcgagcttt
     1381 ttaagagtaa gcaaattatc ggtcttggcc aggaaggcat tcaactgtat gccaacacgt
     1441 tcctgaaaca ggctagcttc cagaccggct cctccctttt gggtgctttc ccattggata
     1501 gaagtattgc caatgttttg aatcgtcaga ccggtcactc tgtccataaa gaccgaagag
     1561 ccgaaataag ttctggccgc gtaataatca atatcgtcgt tacccgaaat atcgtaagcc
     1621 aggttcaatt taagataatc gaccgaacgc agcctgaacc aatcttcgga actcaacacc
     1681 caggctgcag ccgccgaggg gaacaaaccc cagcttactc ctcccatccg gatgcctccc
     1741 tgcacttccc ggccaaatcg ggaagatgtt tccatgctta aacccgcatg caggtaatac
     1801 ttttctgcgt aattataatc caggttggcg taataactca atgagcgcca aacgtcttcg
     1861 gctccgtcta cttttttgaa attaagattg gcgttcatgt tgggggtttt gtcgttgcct
     1921 gtatcgtagc ccatttgggt attgccggaa aagtcgtcgt tcatgtaacg tcctccggcc
     1981 ataaggcggg tgtcgttggc tccccgcttt ttctgccaca acatccttaa gtcggcaaaa
     2041 agagaaatct ggctggaata gagcgatttt ctcaggtttt ctgccgtacc gacattggga
     2101 ataattattt taggtacacc cgtcatgggg atgtaatagt tttcgttggt attgaccaag
     2161 gtgtaactga aatgtccctt gacgctgaga tttttgttaa aggaataagc aggcctaatg
     2221 gccagattga tgaggctgtt ttcgaaataa tttttattac gggcctcgcc gttctcaaaa
     2281 atatagagag gattggccag agatgccctg gaacccaaca cttcgttgag gtaatcgtcg
     2341 gcatcggaca aaaaacccga aagattctta ttgttgtcgt attgataggg gcttaaaaag
     2401 ggcgatttga tcagactcag aaaaccggga gaaaacacgt tggattcttc gggaggcata
     2461 acgccgtcgt ctctgaggtt gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnngagaaaa cacgttggat tcttcgggag gcataacgcc gtcgtctctg aggttgcgtg
     2641 taacgttgct gaaagaacaa tcgaacaagg cgctgagttt gtcgcttata ctgatatccg
     2701 aattaaaacg caggttaaga cgatcaaaat cgttgtaatt caaagtactt tgcgcatcta
     2761 cgtagccaat ggataaattg tatttggcca cctcgtctcc gccctgtact ccaaagctgt
     2821 agttttgagt cagggcttcg cggtagacgt aatcgctcca attggtttcg ttatggtatt
     2881 ggttgtaata gtaataatcg ggatcctctt tcaaaaattt aaattcggta agtttggtat
     2941 cggtagtacc caatagttcc gaagcatagg cacgatattg ggcggcgttc atcatgcggg
     3001 gcagtctggg gataaattcc acgccggcag aggcgcaggc atcgatgcgc gtaaccatgc
     3061 ttttgttgcg tttggttttg ataatgagca ccccgttagc acctttggca ccgtataaag
     3121 aggttccgct tttgagtacc gtaacgcttt cgatgtcgtt taccgacaga ttcgataaaa
     3181 tattgttgta atagccttcg tgcaacaaat ccctgccgta ttgctgatca agtatcacat
     3241 cgtcgagcac gatcaggggc tgggcgttga cgttaagaga attgtatccg ccgacaaaca
     3301 taacggatcc ggcaccggga atacccgaac gcgataccgt acggacttca gcccccaatt
     3361 gcgactgaat ttcagattct atactgatgg ctgagctcaa atcgaaacgc tcggccgatc
     3421 ctgcattttg tacattggtg agtggactga gaaccggaac aaaattttcg gaatacaaaa
     3481 caaggtctac acccgaagtt tgccctttga gtgccatatg ccttaaggcg tagccatcga
     3541 gagtaactgt caggacattt acatagacag gtatctcaag tgtatatcgt ccttccccat
     3601 cggtcatggc tacaaagcga gtattgttgt aagcctgtac tctggctccg accagaggct
     3661 tccctgtaga ggcttcacta actgtacctg ttattgtttt gctttcgaga gtttgtgccg
     3721 ataccgtaaa ggtcgacaag cacaacatta gaacaagcag gcccctgaat ttgatttttg
     3781 catagcagtt tttcatttcc gcaattattt tagatgaggt ttcaggcaaa tacaatcgat
     3841 aagcatctct cgagtataaa aagctgtttc tttcgacgtt atactggttt caattttcaa
     3901 aagaaccgtg gttttttcct ggccgtaatt ggcaaccgga aatttaaaat cggacaaaac
     3961 caacaccgaa tcgagttttg aaggatccgt tatataaata ttgtttccgg tactgtcttt
     4021 gcagctgatg gttctggaat tacccttttc gtcgttgtag gttagggaaa attttaattt
     4081 tgtaggcttg tcgacgtttt cgtgcgttgg attataagtt ttgtatggaa caaaaacgca
     4141 ataaatgtcg taggatgccg acaaagtgtt gggaatttca aaacttaccc agggattgcg
     4201 cgaggtgccg gccggcaaca aatgaatgta acgccggttg gaaatgttaa tgtcgtcttc
     4261 gggttttatg gctctgtcca taatatcaca cagactcgaa tggtaatccc tgccggtggt
     4321 acgctctgct tctactttga tctcctgata aaaattttgt tccggcttgt ataaccaatc
     4381 gtctgtggta tatacagttc cattgctgca aactatttcg gaggttatcc ggctgaagat
     4441 attaccctca tcgaaaggcc gctgatacac atggtatctt ttatcaagcg gccggtattg
     4501 ggcgctgaac aacgaatccc ggggtgaggc ttgcaaattg gcgttgaaaa ccaagtctct
     4561 gaccatcatc attttggtat tgacttgttc cagagagtcg gctttggcca ctttgaaatc
     4621 gtatttaaaa tattgtctta tgctgtcgta agcttcgttc caggccctgt tactcggtgc
     4681 aagcagccaa taggtgctgt cttcgtgatt gataagacct attttttcga gcaatttatt
     4741 ttcgatcgtg accacagaat ccacataaat agttttgccg tcgaccacac cataaggcac
     4801 cgacaacagg ggatcaaaaa caagctcatc aaaggaataa ataaaacttt tcaggctatc
     4861 cagtcccggt tcggttttta agtattcgta aatattgaag aagaaaggat tagctttgtc
     4921 gatgatatgt aatatgccat tggaactcag taaattcttg ttcataatcc cagtctgacc
     4981 gaataaataa gaagtcttgg cttcgttcga acgtaaacgt tccgtttttt tgctgagcaa
     5041 taacacatgt ttgtcagtca ggctcgatac cgggtggctg tagcgggcga tgtggttttt
     5101 cacaaattcc gtcaccacct gctccttttt tccctgattg atcagagtac gcaggctgtc
     5161 cacattgaaa tggccgttgg caggtgccca aaccgtatat acctggtcgg catccaatac
     5221 tttgtcgtat ccgcacagac ggagcaaatc agcgaaatcg gataatacat cgggccttgc
     5281 ctctatgctt tcccataaac tttgagccgg aaccacttcg ggatcggcac cgtagtgctt
     5341 atcccaatcg ttggagcaag cccataccaa aggcaggatc ggtaagagta ttaatccgta
     5401 tctgatgtta aatattgatc tcttcatatt tattctttct atagttggca cttaaagaaa
     5461 gcgacaacag tttaatgacg gtcttctccc tcttcgctgt cgtaaacact tttggggaca
     5521 atttcaatat aatcgaacat aaattctgcg tccaggtcgt cgattacatt acggaaacgc
     5581 aaagtatgcg gttcctgagt aaggtatttg gtcaccagaa tgcggcggaa cagccagctc
     5641 tccgttctga aaaccacata aacattgccg ttcagacgcc gcatactgtc gggagccttc
     5701 atagctttgt ggttgcgcat atccttatcg ttttgcacac cgttatcatc ggttctttcg
     5761 tcggcaatcc agccgatttt gggatcggtg gctcctatgc gtaaatccac gggaataccc
     5821 gtaggttcgc catccacata catttgcgaa atacctctgg tgggcaaagc ggtataaccc
     5881 aacctgattt cgtaggttcc ttcgggcacc ggaggcaaag taatagtgat gtcgtattgg
     5941 ccaaggaaat tgatttcatc cccctggtgg cagtcgtaat tctcgtgctg ataacggtat
     6001 ataaatttgg tctcgctggt caactcggcc ttttcggtat aaccattggg aaaataataa
     6061 ttctgacgat cctggttatg gcgaacattg ttggtcataa attccggaaa caaagctccg
     6121 gtatcgaaac gaaaacgcgt attgaacaca atttcctcga cttcttttga ataggccaaa
     6181 atgtcgtcga tgtaatgata ggtgccgttg atagcattct ggtcggtgct gctcaatccg
     6241 gggggctgga tcaatgcccc tctgatattg aatttattgt cgacacgtct gttcatataa
     6301 agtcccgttg tgccactaat cttgaacatg gtgttcgggc acatcgtttc gtaaaactct
     6361 atggggtcga taatggaagt tataaggcac aaagtagtag tcaatttatt gtagtatccc
     6421 gttcggggca taaagtgata agctacaaag cggttcagag gatttttagg gtgagtaaaa
     6481 tcatcgtcgt ataagttggc gtcctgcgga tagacttcgt cgtatacctt ttttgcataa
     6541 gctatgagat cgttcatgtt gttgatccct ttttgacgat acacctcatc cggttccaca
     6601 aaacccgtat aaccgtattt ccggtatttg ggagcaatgg ccgtttcacg gttgacgtgc
     6661 gaagtgtaat gcagaaccaa tttcggctgc acgtagtcgt aat
//
LOCUS       DAOSTD010000090         6685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_10564_length_6685_cov_16.357014, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000090 DAOSTD010000000
VERSION     DAOSTD010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10564_length_6685_cov_16.357014"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLI37_06400"
     CDS             complement(<1..367)
                     /locus_tag="PLI37_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOQ58674.1"
                     /translation="MNKRNFPLIGLAVFFLLLPCTVRAEKKKQIELMAVTKVVDGDTF
                     WVDDGSEKGKKVRLIGVDTPETLHPQKAPEPYGREASEFTKSLLEGKRVRLEFDVDSL
                     DRYGRLLAYVYLENGLFVNA"
     gene            complement(517..1086)
                     /locus_tag="PLI37_06405"
     CDS             complement(517..1086)
                     /locus_tag="PLI37_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58675.1"
                     /translation="MSSGSRFPLVVSVVSSVLDYLFPFLGEREVGEIQQTKRPKPDYR
                     RQKRALILKYGNPTYNLVLKEQNLNEEIIIFGKTRRIMIQGKLFSHSDILSCTYTDNQ
                     RILKGEATFVGKLDTRKMPSFRGFEGDTNLAAYFEGNNVSNKEIITHNYSVIIKLRDP
                     LASVLKIPMGSNWEKVNELLEAIKFFISG"
     gene            complement(1584..1655)
                     /locus_tag="PLI37_06410"
     tRNA            complement(1584..1655)
                     /locus_tag="PLI37_06410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1620..1622),aa:Arg,seq:cct)
     gene            complement(1771..2490)
                     /gene="recO"
                     /locus_tag="PLI37_06415"
     CDS             complement(1771..2490)
                     /gene="recO"
                     /locus_tag="PLI37_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOQ58676.1"
                     /translation="MLVRSQALVLHQIPYSDAFSVVHLYTREFGRQAYLLTRSRKNSK
                     LRQSLFQPLSLIEYEADHKGSRELQRLKEARSLFAFTGIPMDPVKNAIALFLSEVLYR
                     ALEERESNPALFDFLVQSVEILDLCREGAANFHLVFLIKLTRYLGFYPNLEDKAPGKY
                     FDLQAGTFCSLQPSHRDWLSPEDAQSFAVLLRINFENMPAFRYDRYQRQRILRQTLDY
                     YRLHLSEFPRIKSLDVLQELF"
     gene            complement(2497..>3104)
                     /locus_tag="PLI37_06420"
     CDS             complement(2497..>3104)
                     /locus_tag="PLI37_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ58677.1"
                     /translation="RGALQVKPFSGLNMTVFTGYSFTRDLAMFYNALPVDSGLLNHLS
                     YSSLFMADYVKMNKLYLGLDFNYAYRKTLNLSGKFNYNHYIASSDNAKTDILLESNAR
                     RPWHKPAFEGEALLSLTPGEQTAFFASYHWEGVRYAPDYFNTYRSLPDIHYLSLGASY
                     DITRRLGVFLHLNNVLNQFYEVWNAYPSQGFTAVVGVSYSF"
     assembly_gap    3105..3204
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3205..4193)
                     /locus_tag="PLI37_06425"
     CDS             complement(<3205..4193)
                     /locus_tag="PLI37_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58678.1"
                     /translation="MKKNTLCFLLVLSGLFTMSAVAQTDRDSLIRRDLLLEKEYNPML
                     ETAGKIYRVPETEELKTKGTEVRFSLSEAPTLTPGEYVPLRAAEARTYFPAREQKAYV
                     RLGAGYDWSGLGDLQWNILKGSKHLLDLTAWHRSSFGKVTLQPDLETRAKHIDNRLGL
                     NYQWHLSSAEFRAGLSERFYAWNYYGRSWSNLPLQNRDSLMVPDIQWFSDTKFSLGLE
                     SKPWDKAFDYAFNVDAHYFRLGKALAKDSLAGAGPAETEIDAGLALNYQFNDNLDIGL
                     DARFRAFFYQRGLSSQRFSLADHMQYDPLFPAYNDAPANVLWFEMNPHATYSLG"
     gene            complement(4196..>6685)
                     /locus_tag="PLI37_06430"
     CDS             complement(4196..>6685)
                     /locus_tag="PLI37_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ58679.1"
                     /translation="STYYLAFIDYRQGRTEQALEGFGKIAAHPEYERIVPFYIMQLYF
                     DQGKNKEMLEIAEKLLQEDPNESERIELYRLSAAGYYELNDHALSQEYYGKYLRYNPE
                     LLRSDAYRIGINHYVAKEYEQAAKYFLQVSQEGDALAQNAEYHLGLCYLQQQKFDQAR
                     MSFEQASLSDQDKEVKESALYNYALLCYQTSFSPFNEQVRAFERILTEFPNSRYADRV
                     YGHLVDAFLSTKNYQASLDVLNKIADPGKDLLETKSKLLFLMGMDRFNNAAYGEALTY
                     FNSSIQLSTQLRLEAPETYFWRGETHYRLENLRAASNDYLRFLNSPNASTYKAWQLAH
                     YNLGYSYYNLKQYPEALNWFLKYSQFKEIRNDNTYPDVLNRIGDCYFHARNYDRAEEY
                     YNMSDKLSETGNDYAVFQRGFSMGLQKRHEEKARLFAGFEERFPLSDYLDDALYEQGR
                     AYIALNRPAEALPVFEKLMRKFPNNILARKAGIQIGLLEYNRNNTEAAIAAYKKVINE
                     YPGSQEAQTALNDLKMIYVAVNRVPEYLNYVDNLGSGVRIERSEKDSLSYLAAERLMV
                     DGKNQEAIKGFQDYINAYPQGMFRTEAYFHAGNLLLLSNETSRALPYFQEVIKQTGHR
                     FVPEALVVLAEHEYKQKEYARALEHYQSLDVLAEDRSVRIGTRSGILRCLYALGRNES
                     IPAAADKLLAEENLSPSLQREALFRKTIALMELNRTDEALSDLQRLAEESQTAFGAEA
                     RFRLAEYWFNKNDLKKAEEIAQSFLRDGTSQSYWLARCFVLLSDIYIQRKDYFQARQY
                     LMSLKENYRAEDEIAGMIQTRLDNIAQLSNE"
BASE COUNT         1796 a         1560 c         1464 g         1765 t
ORIGIN      
        1 cggcattgac aaaaagtccg ttttcaaggt aaacataggc cagtaagcgg ccgtagcgat
       61 caagactgtc cacatcgaat tccaatctca cccttttacc ctcgagcaga gacttggtaa
      121 attcggaggc ttcacgacca tagggttccg gagctttttg cggatgcaat gtttcgggcg
      181 tatctacacc aattaatcga acctttttgc ctttttcgct accatcgtcc acccaaaacg
      241 tgtctccatc caccactttg gtaacggcca ttaattctat ctgctttttc ttttcagccc
      301 taacagtaca gggcaaaagc aagaagaaaa cggccagacc gattaaaggg aaatttcttt
      361 tattcataat aaaatcaagt aatctcccca aagttagaaa ttcatttgat gatttatagc
      421 cggacaaata cttattcggg caattcttct tttgtgtggg ttgggctcac tatcctctta
      481 tattttacat aataaatacc ccaatataat agggaactac ccggatataa agaattttat
      541 cgcctccaac agctcattta ctttttccca attcgatccc atggggattt ttaggacgga
      601 tgccaaggga tctctgagtt ttatgattac actataattg tgagtaatta tttccttatt
      661 ggaaacattg ttgccttcga agtatgccgc caggttggta tcgccttcga aacctctgaa
      721 cgaaggcatt ttacgggtat ccaatttacc cacaaaagta gcctcgccct taagaattct
      781 ttgattatcg gtatatgtac acgacaaaat atcggagtgg gagaatagtt ttccctgtat
      841 cattatccgt cttgtcttcc caaagattat tatttcctcg ttcagattct gctccttaag
      901 taccaggtta taggtggggt tcccatactt taaaattaac gccctttttt gcctgcggta
      961 atccggcttc ggtcttttcg tttgttgtat ttcccccacc tccctttcac ctaagaaagg
     1021 aaacaaataa tccaacacag acgaaacaac cgaaacaacc aaagggaacc tactaccaga
     1081 actcatcaca actaatttaa acggccaaaa aatattcatt ccactaaatg tctcgatgcc
     1141 gaaacctgac gcacacagct ctctttttct tgaagcatcg aaggacgtat acaaagatgc
     1201 atcgaaaaac tgttaaattt tctgtaatcc gaaaaagttt cgctgtattt tgttgttatt
     1261 ctgatggtta acatcaaaaa gtttcgctta aatccggaca agaaaaatac ttgtgtgtaa
     1321 agtcaaaatt ataaatcctc gatttcatcg atgtttttta tttttatgaa atttaccgct
     1381 tttaccccct ttttcgaatt aaaatggctg aatcaatcgg acaacagccc ggagattcgg
     1441 ccttcagatt ccggattgct aatttaattt tcagttaaac gggaagcaat cttattcagc
     1501 ctcttcatat attttacaaa cagagtgata caaaaaagct gttccaaccc gtgagtcaaa
     1561 acagccttct tgttgcttca aagtagtccc gccgagaatc gaactcgaat ctaaagttta
     1621 ggaaacttcc gttctatcca ttgaactaca ggaccaagat acttactgat tcgggcgcaa
     1681 aaataaggct aaatttccga ttgaacaaga ttatcttacc cgatgcctcc gacgatggaa
     1741 aatttcttac tgcctatttc gatgcaatga tcaaaataat tcctgcaata catccaatga
     1801 ctttatcctg gggaattcgc tcaaatgcaa acggtaataa tcgagggttt ggcgcaatat
     1861 gcgctgacgt tggtaacggt cgtagcgaaa agcaggcata ttctcgaagt taatgcgcag
     1921 caacacagca aagctctgag cgtcttcggg cgacaaccaa tctctgtgcg aaggttggag
     1981 cgaacaaaaa gtaccggcct gcaagtcaaa atatttaccg ggggccttgt cttccaaatt
     2041 cggataaaag cccaggtaac gggtaagttt aatcaaaaat accaggtgaa aatttgcggc
     2101 accctcgcga caaagatcca gtatttctac agattgtacc agaaaatcga acaaggccgg
     2161 attggactct ctttcctcca gggcacggta caaaacctcc gataaaaaca aagctatggc
     2221 gttttttacc ggatccatcg gaattcctgt aaacgcaaac aggctgcggg cttctttgag
     2281 ccgctgcaat tctcggctcc ccttgtgatc ggcttcgtac tcaatcaggg acagaggctg
     2341 aaagagcgac tgcctcagct tgctgttttt gcgacttctt gtcaatagat atgcttgccg
     2401 tccaaactcc ctggtataga gatgcaccac cgaaaaggca tcgctgtagg gaatctgatg
     2461 caacacaaga gcctgacttc gaaccagcat acgagtttag aaagaatagg aaacccccac
     2521 gacggccgtg aagccctgag agggataagc attccatact tcatagaatt gattcagaac
     2581 attgttcaga tgcaaaaata cgcccagccg gcgggtaatg tcgtaagaag ctcccagcga
     2641 aagataatgg atatcgggca aactccggta ggtgttaaaa taatcgggag catagcggac
     2701 accctcccaa tggtaggagg caaaaaaagc agtctgctct cccggagtca ggctgagcaa
     2761 agcctctcct tcgaaagcag gcttatgcca gggcctgcgg gcattgcttt ctaacaagat
     2821 atctgtcttg gcgttgtccg acgaagctat gtaatgatta tagttgaatt taccgctcaa
     2881 atttagggtc tttctatagg cataattaaa gtccaggccc agataaagct tgttcatttt
     2941 tacataatcg gccataaaca gactactata actcaagtga ttcaacaggc cgctgtctac
     3001 gggcaaagca ttgtaaaaca tcgcaaggtc tctggtaaaa gaatagccgg tgaatacggt
     3061 catattgaga cccgaaaacg gcttaacctg caaagctccc ctcannnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnncccagg gaataggttg cgtggggatt catttcaaac
     3241 cataacacat tggccggcgc atcgttatag gccgggaaaa gcggatcgta ctgcatatga
     3301 tcggccaggc tgaatctttg tgaagaaagc ccgcgttgat aaaagaaggc tctgaatcgg
     3361 gcatccaaac ctatatccaa attatcgttg aattggtagt ttaaggcgag accggcatct
     3421 atttcggttt cggccgggcc ggcaccggcc aggctgtctt tagccagggc tttacccaaa
     3481 cggaaataat gagcatccac attgaaggcg taatcgaacg ctttgtccca aggtttcgat
     3541 tctaatccga ggctgaactt ggtatccgaa aaccattgta tatccggcac cattaagctg
     3601 tctctgtttt gcaagggcaa attgctccag gaacggccgt agtaattcca ggcataaaaa
     3661 cgctccgaca agcctgccct gaattccgct gaactcaaat gccattggta attgagcccc
     3721 aaacgattgt ctatatgctt ggctctggtt tccaaatcgg gttgaagagt cacttttccg
     3781 aaagacgaac gatgccaggc ggtcaaatcg agcaaatgtt tgctcccttt caatatgttc
     3841 cattggaggt cgcccaaacc cgaccaatcg tagccggctc cgagcctgac gtaagccttt
     3901 tgctcccggg ccgggaagta ggtgcgagcc tcggcagccc gcaaaggaac gtattctccc
     3961 ggagtcagcg taggtgcctc cgacagggaa aaacgcactt cggttccctt ggttttaagt
     4021 tcttcggttt cgggcacacg gtaaatctta ccggccgttt cgagcatggg attgtattct
     4081 ttttcgagca ggaggtctct tctaatcagg ctgtcccggt ctgtctgagc taccgcggac
     4141 atcgtgaaca ggccggataa aaccagcaaa aaacagagag tattcttttt cataatcatt
     4201 cattactgag ttgggcgata ttatccaaac gcgtttgaat cataccggcg atttcatctt
     4261 cggcccggta attttctttc aagctcatca aatactgcct ggcttggaag taatctttac
     4321 gttgaatata aatatcggac aggagaacaa agcaacgggc cagccaatac gattgcgaag
     4381 ttccgtccct aaggaaagac tgggcaattt cttcggcttt tttcagatcg tttttgttga
     4441 accaatattc ggccagccgg aaacgggcct cggcgccgaa agcagtctgt gactcttcgg
     4501 ccaggcgttg caggtcggag agggcttcgt cggttctgtt cagctccatc aaggcgatgg
     4561 ttttccggaa cagtgcttcg cgttgcaggc tgggtgagag attttcttca gcaagcaact
     4621 tgtcagcagc agccggaatg ctttcgttac gcccgagcgc atacagacaa cgtaatattc
     4681 cgcttctggt gcctatgcgg acactgcggt cttcggccag cacatccaaa ctctgataat
     4741 gttccaaagc ccgggcgtat tctttttgtt tatactcatg ctcggccaac accaccaagg
     4801 cctcgggtac aaaacgatgg ccggtttgtt taatcacctc ctgaaaatag ggcagagccc
     4861 ggctcgtttc attgctaagc aaaagcaaat ttccggcgtg aaaataagcc tcggtacgga
     4921 acatcccctg aggataagca ttgatataat cctgaaaacc ttttatggct tcctgattct
     4981 taccatcaac catcaggcgt tcggcagcca gatacgacaa agaatctttt tcgctgcgtt
     5041 ctatccgcac gccgctgccc agattgtcga catagttcaa atattcaggc acccggttga
     5101 cggccacata aatcatcttc aggtcgttaa gggcggtttg agcttcctgg ctgcccggat
     5161 attcgtttat tacctttttg taggcggcaa tggctgcctc cgtgttatta cggttatact
     5221 ccaacaaacc tatctgaata cccgctttgc gggccagtat attgttggga aattttcgca
     5281 tcagtttttc aaaaacaggt aaggcttcgg ccggacgatt cagagcaata taagcccggc
     5341 cttgctcgta aagggcatcg tccaggtaat ccgacagggg aaaacgttct tcaaaaccgg
     5401 caaaaagacg ggctttctct tcgtgccgct tctgcaggcc catactgaac cctctctgaa
     5461 acacagcgta atcgttgccg gtttccgaaa gcttgtcgct catgttgtag tattcttcgg
     5521 cccgatcgta gttgcgggca tggaaataac aatcgccaat gcggttcaaa acatcgggat
     5581 aagtattatc gttgcgaatt tccttgaact gactgtattt taaaaaccaa ttcaaagcct
     5641 cgggatattg tttaagattg tagtaggaat aacccaggtt gtaatgggcc aattgccagg
     5701 ctttgtaggt cgaagcatta ggtgagttaa ggaaacgcag gtaatcgttg gaagcagccc
     5761 tgagattttc gaggcgataa tgggtttccc ccctccaaaa ataagtttcc ggagcctcca
     5821 gtctgagttg ggtgctcaac tgaatcgaag agttaaaata agtcaaggct tctccgtagg
     5881 ctgcgttgtt gaatctatcc atacccatca aaaaaagcaa cttgcttttg gtttccagca
     5941 aatccttgcc gggatcggca attttgttca acacatccaa agaagcctgg taatttttgg
     6001 tacttaagaa agcatcgacc aaatgcccgt aaacacgatc ggcataacgg gaattgggga
     6061 attccgtcag gatgcgttcg aaagccctta cctgttcatt aaaaggcgaa aaagaagttt
     6121 gatagcagag caaggcgtaa ttgtaaagag cgctctcttt tacctccttg tcctggtcgc
     6181 tcaacgaagc ttgctcgaaa ctcatgcggg cctggtcaaa cttttgctgt tgcagataac
     6241 acaaacccag gtggtattcg gcattttgcg ccagcgcatc gccctcctgg cttacttgca
     6301 ggaaatactt tgccgcctgt tcgtattctt tggccacata atgattgatg ccaatgcggt
     6361 aagcgtccga acgcaaaagc tccggattgt atctgaggta cttgccgtag tattcctgcg
     6421 aaagggcgtg atcgttgagt tcataataac cggcggccga aagacggtat agctcgatac
     6481 gctccgattc attgggatct tcctgcagaa gtttctcggc aatctcgagc atttctttgt
     6541 ttttgccctg atcgaaataa agctgcatga tatagaaggg cacaatgcgt tcgtattcgg
     6601 gatgggcggc aattttgccg aatccttcga gggcttgctc tgtccggccc tgacgataat
     6661 ctataaaggc caggtaatag gtcga
//
LOCUS       DAOSTD010000091         6638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_10664_length_6638_cov_10.332979, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000091 DAOSTD010000000
VERSION     DAOSTD010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10664_length_6638_cov_10.332979"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLI37_06435"
     CDS             complement(<1..241)
                     /locus_tag="PLI37_06435"
                     /inference="COORDINATES: protein motif:HMM:NF025041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HOQ58680.1"
                     /translation="MKTNVVKVKLWGTNVGYLSWDSREEVAVFEYDPSFLKLGWDIAP
                     LTMPVNASRSRKQMPWLGNKKHNTHRPPFVYRQPGN"
     gene            complement(238..534)
                     /locus_tag="PLI37_06440"
     CDS             complement(238..534)
                     /locus_tag="PLI37_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ58681.1"
                     /translation="MYSDLYEKSGSEIIRELGKRYAGYRKRMGFTQKEIASKSGLSLF
                     TISSFENGSLTGITIASFIKLLRAIDRLEEIEKLLPELPESPRVLFKKQQKNKL"
     gene            complement(691..1794)
                     /locus_tag="PLI37_06445"
     CDS             complement(691..1794)
                     /locus_tag="PLI37_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ58682.1"
                     /translation="MSAFIVLLQKEFTQIRRNSFLPKLIVAFPVIVMLIAPLLMTMDV
                     RNVNVAIVDLDRSTTSHRIVSHIKASEYLSLVGATAEFDLALRDLEKGKVDVIVQIPN
                     YFERDMAVATAEKISITANAVNAIKALIGTQYLVQTIARTVVELSGEKSPNQLSELVT
                     IQNRFNPTLNYRHYMIPALMIMIFILMCGFLPALSIVGEKETGTMEQINVTPVSRLTF
                     ILSKLLPYWIIGIFVLTMAMIVAHLVYGLIPVGSVGLIYFGAVLFILIISGFSLTVAN
                     FSDTMQQTMFVMFFFIMIFMLMSGLLTPIDSMPAWAQKITFILPPRYFVDILRSVYLK
                     GTSLVEQWYNFAALGVFTVIFNILAAVTYRKQA"
     gene            complement(1799..2893)
                     /locus_tag="PLI37_06450"
     CDS             complement(1799..2893)
                     /locus_tag="PLI37_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ58683.1"
                     /translation="MKNNFLPFVKKESLHILRDKRTMLIVMLIPVILMVMFGFAISTE
                     VNNINIAVVAPERTGAVKDVVGRIAHNDYFTFYGYIDQDQIDKKLRSGKVSVVAVFTS
                     NFDRRMEQISAGLPSKPVIQFIVDASNVNTASASIAYLQSILPGTASQQAMFETRMLF
                     NPQMKSAYNFVPGIMGLIFILVCAIMTSVSIVREKEVGTMEVLLVSPVRPVKIVFAKM
                     IPYFVISCVILAMILLLAHYLLDVPMSGGIWGIFSLSLLYIVLSLSLGLLISTVTKTQ
                     VAALLISAMVMLVPVLLLSGIIFPIENFPKFFELLSNIIPARWYIAAIRKMMIQGAAF
                     TAVWKEGIVLLGMTVVFIGISLKKFNDKLE"
     gene            complement(2898..3638)
                     /locus_tag="PLI37_06455"
     CDS             complement(2898..3638)
                     /locus_tag="PLI37_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ58684.1"
                     /translation="MSKTVISVRNLTKRFGNFTAVNNISFDVYRGEIFGFLGANGAGK
                     TTAMRMLCGLSYPTSGSGTVAGFDITREGEMIKRHIGYMSQRFSLYNDLTVGENIKLF
                     AAIYGLSKQNTGKRTDELLDRLNFSSERNTLVGSLPLGWKQKLAFAIATVHRPEVVFL
                     DEPTSGVDPITRRQFWEMIYEAAEGGTTIFVTTHYMDEAEYCSRVSIMVDGEVRALDT
                     PARLQQQFAAESMNEVFRTLARDAERGE"
     gene            complement(3635..4534)
                     /locus_tag="PLI37_06460"
     CDS             complement(3635..4534)
                     /locus_tag="PLI37_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ58685.1"
                     /translation="MDVIEVSGLSKSFGKVKALDSVSFSVKRGELFGLIGPDGAGKTT
                     LFRLLTTLLNPDTGTASIDGLDIIKDYLKIRSRVGYMPGRFSLYPDLTVEENLIFFAS
                     LFGVKVEDSYDLVAPIYKQIEPFRTRQAGKLSGGMKQKLALSCALIHHPSVLFLDEPT
                     TGIDAVSRGELWDMLQKLKEKGITILVSTPYMDEASLCDRIALCNEGRILGIDSPQGI
                     VEKFNLKLYAIRADNMFTLLEAARQVEGVAECYPFGQTHHLIADEKFDIHRFTEQLEA
                     LSNLTIKPTKPAIEDVFIKLMKQ"
     gene            complement(4536..5423)
                     /locus_tag="PLI37_06465"
     CDS             complement(4536..5423)
                     /locus_tag="PLI37_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOQ58686.1"
                     /translation="MKRVVYIAAIILTVSSCGKKAEFDAQGTFEAIEVVVSSEATGRI
                     LSFEIEEGMPVKAGMELGLIDSLQLHLQYKQLSAQLSALLDSRPDIKAQVAALREQIA
                     KQKRELKRVGNMLKDAAATQKQYDDIQAQIRILESQLSATLSSLDKSTATINNNALAL
                     EAQMAALADRMAKCRIASPVDGTVLVKYAQAGELATMGRPLMKIADLNNIYLRAYFTS
                     NQLAHIKLGDEVTVTADFGGDERYDYAGRITWISPESEFTPKTIQTKDSRANLVYAVK
                     IAVKNDGRLKIGLSGNVKL"
     gene            complement(5427..>6638)
                     /locus_tag="PLI37_06470"
     CDS             complement(5427..>6638)
                     /locus_tag="PLI37_06470"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOQ58687.1"
                     /translation="CRRLAREHYPEIRQYNLVTRTEQFNVSNAAKAWIPQVVLSGQAT
                     YQSAVPAYPEALKNMMQANGVDMSGTGMNKDQYRIAVDVTQNIWDGGQSKASRAMAEA
                     EAAEQRSRADVSLYDLQSRVDGLYFGILLLDERMEQTKAMIEVLGSNLNRMRTYHENG
                     VALQSDVDVMEAELLTAQQQLGQVESSLTSYRRMLEIFIGRSLISDKLERPVMPIIQD
                     YTSTRPEFALLDAQENRLTAQRRAINSSVMPRFGAFAQGYYGYPGLDIFKDMTSTEWT
                     LNGIVGVRMLWNISAFYTKNNNLEKISIAQQQLAVQREILQFNTRMLTTQDDGEIARL
                     RKAVENDARIVELRRSVRMAAESQLKNGVIDATDLLRKITDETTAALNRNMHEIELLQ
                     AIYKLKHTLNE"
BASE COUNT         1825 a         1565 c         1441 g         1807 t
ORIGIN      
        1 cattgcccgg ctgccgataa acgaaaggtg gtcgatgggt gttatgtttt ttgttaccta
       61 gccaaggcat ttgcttacga ctgcgggacg cgttaaccgg cattgtgaga ggggctatat
      121 cccatcccag cttgagaaaa gaaggatcgt attcaaaaac agccacctct tccctgctat
      181 cccaggaaag atacccgaca ttcgttcccc acagctttac tttaacgaca tttgttttca
      241 taacttattt ttttgctgtt ttttgaacaa tactcttgga ctctcgggta attcgggcaa
      301 gagcttttcg atttcttcca aacgatctat cgctcgaagt aatttaataa acgatgctat
      361 ggttattccc gttaaagagc cgttttcaaa tgaactgatg gtaaacaggc ttaaaccgga
      421 tttcgaagct atctccttct gcgtaaaccc cattcgcttg cggtaaccgg catatcgttt
      481 ccccaattcc cgtatgatct cgcttcctga tttttcgtac aaatcgctat acatttccag
      541 ttatatttaa tcacaaaata gatatatcta tattatttcg caaatatatg aatatttatt
      601 gatatatcta ttccattttg gggagaaaag cttgatttgt aaaggagctt cacaaataag
      661 ttatgcgaaa ttaagcccgg ctatgcgagt ttaggcctgc ttcctataag taacagcagc
      721 gagaatattg aagataaccg taaaaacacc gagagctgcg aagttgtacc attgttcgac
      781 aaggcttgtt cccttcagat agactgaacg taatatatcg acaaaataac gcgggggcag
      841 aataaaggtg attttctgcg cccaggcggg catggaatca atgggcgtaa gcagtccgct
      901 catcagcata aagatcataa taaagaagaa cataacgaac attgtctgtt gcatcgtatc
      961 ggagaaattg gccaccgtga ggctgaatcc ggaaataatc agaataaaca ataccgcacc
     1021 aaagtagata agcccgacag aaccgacggg tataagccca tagacgagat gcgcaacaat
     1081 cattgccatc gtaagcacga atatgcctat aatccaataa ggtaacagct tcgacaaaat
     1141 gaacgtaagg cgactcacgg gggtaacatt gatctgctcc atcgtgccgg tttccttttc
     1201 tcccacgatg ctcaatgcag gtaaaaagcc gcacatgagg ataaagatca taatcatgag
     1261 ggcaggaatc atataatgcc ggtaattgag tgtcggattg aagcgatttt gtatcgttac
     1321 caattcggac aattggtttg gacttttctc gccgctcaat tcaaccactg tacgagcgat
     1381 ggtttgcaca aggtattgcg tgcctatgag agctttgatg gcgttcacgg cattggctgt
     1441 tatgcttatt ttttcggctg tggcgactgc catatcacgc tcaaaataat tgggaatctg
     1501 cacgataaca tccaccttgc ctttctctaa gtctcgcaac gcaagatcaa attcagcagt
     1561 tgctccaacc aacgacagat attcggaagc cttaatatgt gatacaatgc ggtgagatgt
     1621 tgtcgagcgg tcaagatcaa caatcgcaac attcacattg cgcacatcca tggtcataag
     1681 aagtggagca atgagcatca ctattacggg aaaagccact atcaatttgg gcagaaagga
     1741 attgcggcga atctgcgtga actctttttg taataatacg ataaacgcac tcatgtggct
     1801 actctaattt atcgttgaac tttttcagcg atatgccgat gaatacaacg gtcataccga
     1861 gcaatacgat accctctttc cacactgccg taaaagccgc cccctgaatc atcattttgc
     1921 gtatggcagc aatataccaa cgtgcaggga ttatattcga cagcagttca aagaatttgg
     1981 ggaaattttc gatagggaat ataatacccg aaagcaagag tacaggcacc aacattacca
     2041 tggccgaaat aagaagtgca gctacctgtg ttttggttac agtcgaaata agaagcccga
     2101 gcgagagcga caacactata tagagcaacg acaatgaaaa gataccccaa atgccgcccg
     2161 acatcggtac atcgagcaga taatgagcga ggagcagtat cattgccaat atcacacagg
     2221 aaataacgaa gtaaggaatc atcttggcaa agacgatttt taccggacgt acgggcgaaa
     2281 cgagaagcac ctccatggtt cccacttctt tttcacgtac aatcgatacc gaagtcatta
     2341 tggcgcaaac aaggataaaa atcaaaccca taataccggg tacgaagttg taagcactct
     2401 tcatttgcgg attgaaaagc atcctggtct cgaacattgc ctgctgtgat gccgtgccgg
     2461 gcaggatact ctgcagatag gcaatagacg cacttgccgt gttgacgttg gatgcatcga
     2521 ctatgaattg tataaccggt tttgaaggca gtccggctga aatctgctcc atacgccgat
     2581 cgaaatttga ggtaaaaacg gcgacaacac tcactttgcc tgaacgcaat tttttatcta
     2641 tctgatcttg atcaatataa ccgtagaagg tgaagtagtc gttgtgtgca atacgaccga
     2701 caacatcttt cacagctccg gtacgctccg gagccaccac agcgatgttt atattattca
     2761 cctcggtaga tatggcaaaa ccgaacatta ccatcaatat tacagggata agcattacga
     2821 taagcatggt acgcttatcg cgcaaaatgt gtaacgactc tttttttacg aacggtagaa
     2881 agttgttttt catggcttca ctctcctctt tccgcatcgc gagccagtgt acgaaagact
     2941 tcgttcatcg actcagcggc gaattgttgt tgcaaacgag cgggtgtgtc gagggcccta
     3001 acctcaccgt cgaccataat cgacacacgc gagcagtact ccgcctcatc catatagtgg
     3061 gtggtaacaa atatcgttgt tccaccctct gctgcttcat aaatcatttc ccaaaattgg
     3121 cgacgagtaa tcgggtcaac cccactcgta ggttcgtcta aaaaaaccac ttcgggacga
     3181 tgaaccgtgg caattgcaaa cgccagtttt tgcttccacc ccaatggtaa ggagccaacg
     3241 agtgtattgc gctccgacga aaaattaagc ctgtcaagta gttcatcggt gcgcttcccg
     3301 gtattctgtt tcgagagtcc ataaatagct gcaaaaagct tgatattttc gccaaccgta
     3361 aggtcattgt aaagagaaaa acgttgactc atgtagccga tatggcgctt gatcatctcg
     3421 ccttcgcgtg taatatcaaa acctgcaacc gtaccgcttc ccgaagtggg atagctcaat
     3481 ccgcagagca tacgcatggc ggtagttttt cccgccccgt tggcaccgag aaaaccaaaa
     3541 atctcacccc ggtaaacatc aaaagagata ttgtttacag cagtgaagtt gccaaaacgt
     3601 ttggtaaggt tgcgtactga aattactgtt ttgctcattg tttcatcagc tttataaata
     3661 catcttcaat agcaggtttc gtaggtttaa tagtaagatt actcaaggct tccaactgct
     3721 ctgtaaagcg gtgtatatca aacttctcat ctgcaataag gtgatgcgtt tgtccaaacg
     3781 ggtaacactc tgccacaccc tcaacttgtc gtgctgcttc gagcagagta aacatattgt
     3841 cggcacgaat agcataaagc ttcaaattga atttttcaac aattccttgc gggctgtcta
     3901 ttcccaaaat acgtccttcg ttacatagtg caatgcggtc acaaagtgaa gcttcgtcca
     3961 tataaggagt agataccaga atggtaatcc ctttctcttt gagtttttgt aacatatccc
     4021 aaagttcacc acgcgatacg gcatcaatac ctgttgtcgg ttcgtcgaga aagagcacgc
     4081 tcggatggtg aattaatgca caagagagag cgagtttttg tttcatgcca cccgatagtt
     4141 tacccgcctg gcgtgtacga aaaggttcaa tttgtttata gatcggagca acgaggtcgt
     4201 atgaatcttc aaccttaacg ccaaagagtg aggcaaaaaa tataagattt tcttccaccg
     4261 tcaggtccgg ataaagtgaa aatcgaccgg gcatataacc cactcgcgaa cgtatcttaa
     4321 gatagtcttt tataatgtct aaaccatcga tcgatgccgt gcctgtatcg ggattgagca
     4381 atgtagtaag cagacgaaaa agcgtggtct tgcctgcgcc atcaggacct ataagaccaa
     4441 atagttcgcc ccgcttaacc gaaaacgaga ccgaatcgag agctttgact tttccgaaac
     4501 tctttgagag accgcttacc tcgataacat ccatactaca atttcacatt gcccgatagt
     4561 ccgattttaa ggcggccatc gttctttaca gcaattttta cggcatagac cagatttgcc
     4621 cgcgaatctt tcgtctgtat ggtttttggc gtaaactcac tttcgggcga aatccatgta
     4681 atacgacccg cataatcgta acgttcatca cctccgaagt cggctgtaac tgtaacttcg
     4741 tcgcccagtt ttatgtgggc aagttgattg gatgtgaaat aggcacgaag ataaatgttg
     4801 ttgagatcgg caattttcat caagggccta cccattgttg ccaactcacc tgcttgggca
     4861 tacttcacca gtaccgtacc atcaacgggc gacgctatcc ggcattttgc catacggtcg
     4921 gcaagagcag ccatctgtgc ttcgagagct agcgcattgt tgttgatggt agcggtgctt
     4981 ttatcgaggc ttgagagcgt tgccgaaagt tgactctcga gaatacgtat ctgtgcttga
     5041 atgtcgtcgt attgtttttg cgtggcggca gcgtctttga gcatgttccc cactcgctta
     5101 agctcccttt tttgtttggc tatctgctca cgtagtgctg cgacttgcgc ctttatatcc
     5161 ggacggctgt ccaatagcgc cgaaagttgt gccgacaact gcttatactg aagatggagc
     5221 tgcaaactat cgatcaagcc cagctccatg cctgctttaa cgggcatacc ctcttcaatt
     5281 tcaaaactca aaatgcggcc tgtagcttcc gatgacacca ccacttcgat agcctcaaaa
     5341 gtgccctgcg catcaaattc cgctttttta ccgcacgatg atacggtaag tattattgcg
     5401 gcgatataaa ccactcgttt catatcttat tcgtttaatg tatgtttcaa tttataaata
     5461 gcttgtagca attctatctc gtgcatatta cgattgagtg ccgctgttgt ttcgtcggtg
     5521 attttacgca gtaaatccgt ggcatcgata acgccgtttt taagctgtga ttccgcagcc
     5581 atgcgtaccg agcgacgtaa ttccacaata cgggcatcat tctcaaccgc cttacgaagt
     5641 cgggcaatct cgccatcgtc ctgtgtggtc agcattctgg tattgaattg taatatttcg
     5701 cgctgaacgg ctaattgttg ttgagcaatg cttatttttt caagattatt atttttggtg
     5761 tagaatgcgc ttatattcca caacatgcgc acgcccacga tgccattcag ggtccattcc
     5821 gtagaggtca tgtctttaaa tatatccagg ccggggtagc cgtagtagcc ttgcgcaaag
     5881 gctccaaaac gaggcatcac cgatgagttg atagcccttc tttgcgcagt taacctgttt
     5941 tcctgagcat cgagcagagc gaattcaggg cgtgttgaag tgtagtcctg aataatcggc
     6001 atgactggtc gttccaactt atcggaaatc agcgaccgac cgataaaaat ctccaacata
     6061 cggcgatagc ttgttaggga ggattccacc tgacccaact gttgctgagc ggtaagcaat
     6121 tctgcctcca tcacatcaac atccgattgc aacgcaacgc cgttttcatg ataggtacgc
     6181 atacgattta gattacttcc caatacctca atcatagcct tggtttgttc catacgttca
     6241 tcgagcagta aaatgccaaa gtacagacca tccaccctcg actgcaaatc gtagagtgat
     6301 acatctgcac gactgcgctg ttcggctgct tccgcttcgg ccatagccct actcgctttt
     6361 gattgacctc cgtcccagat gttttgagta acatcaacag caatcctgta ctggtcttta
     6421 ttcatacctg tacccgacat atccacacca ttggcttgca tcatgttttt gagggcctcg
     6481 ggataggcgg gaactgccga ctgatacgtt gcttgccccg acaaaaccac ttgtggtatc
     6541 catgcttttg ccgcattcga tacattgaac tgttctgtcc gggtaacaag attgtattgc
     6601 ctgatttcgg gatagtgctc ccgtgcgaga cgacggca
//
LOCUS       DAOSTD010000092         6337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_11312_length_6337_cov_14.495702, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000092 DAOSTD010000000
VERSION     DAOSTD010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11312_length_6337_cov_14.495702"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PLI37_06475"
     CDS             <1..854
                     /locus_tag="PLI37_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOQ58688.1"
                     /translation="QEEATPADSTVASGPFLVPKNIDLNVNTSIMNAYVGNQIATNLT
                     GRLYVKDDALVLEEIGFVSAAARLKLTALYRSPRPNHLYLGLDYHMVDIQIDELLSMF
                     PDLDTIMPMLSSFKGQGEFHLAAETYLDKNYNLKKSTLRGASSIRGDNLVLMDSETFG
                     KISNILMFNKKTENKIDSLSAEFTVFKNEVDVYPFTIVMDKYKAVVGGRHNLDMSFDY
                     HISLLESPLPMRLGVNIRGTMDDLKIRPAACKYPENYRPVSRRIVDAQKLELRKLIRE
                     TLIKQME"
     gene            complement(904..1938)
                     /gene="rhaT"
                     /locus_tag="PLI37_06480"
     CDS             complement(904..1938)
                     /gene="rhaT"
                     /locus_tag="PLI37_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HOQ58689.1"
                     /translation="MNTLIGLLVIAVGSFGQSSSYVPINKIKNWSWESFWLVQGVFAW
                     LVFPFLGALLAVPQGSTLGALLSVDPAASWKAIGFGALWGVGGLTFGLSMRYLGVALG
                     QSIALGTCSAFGTLIPALFFDKQDLLHGDGLILLLGVCITLAGIAVIGYAGGLRAQNM
                     TEEQKKAAIKDFALTKGLLVALLAGVMSACFALGLSAGQPIADAAKSVGANDLFATLP
                     TTLMVTLGGFFTNAVYCLSQNAKNKSFKDYTSNNRKIWVNNLLFCSLAGLLWYSQFFG
                     LSVGKSFLAENAIMLAFSWSILMALNVTFSNVWGILLKEWKGVSTKTIVVLILGLLIL
                     VFSVFFPNLF"
     gene            complement(1941..3197)
                     /locus_tag="PLI37_06485"
     CDS             complement(1941..3197)
                     /locus_tag="PLI37_06485"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOQ58690.1"
                     /translation="MVKEKLVQQAYEIARERYAALGVDVDAALNKLQKISLSLHCWQA
                     DDVSGFENPDGELTGGIQSTGNYPGKARNIDELRADIKKATSFIPGKHRLSLHAIYGD
                     FQGKFVERDQLEPKHFASWMQWAKENEMKLDFNSTSFSHPKSEALTLSSPKKEIREFW
                     IEHTKRCRAIAEAMGVYQGSPCVMNLWIHDGSKDMTVNRLEYRRILEQSLDEIFAQKY
                     NNMKDCIESKLFGIGAEYYTVGSHDFYLGYGVKNQKIVTLDTGHFHLTESIADKISCL
                     LLFTPEIMLHVSRPIRWDSDHVTLMSDDTLELAKEIVRANALDRVNIGLDYFDGSINR
                     IGAYVIGSRATQKCFLHALLEPIEILRKYESKEQGFERLALLEECKGLPWNAVWDYFC
                     LKNNVPVGEDFIPEIQQYEKEVTSKR"
     gene            complement(3223..>4668)
                     /locus_tag="PLI37_06490"
     CDS             complement(3223..>4668)
                     /locus_tag="PLI37_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOQ58691.1"
                     /translation="LGATSGRAILGTLGGGKLETKELTRFPNLIMPIHGKSYWNIFGL
                     YESLKAGLKAAAATGQKIDAIGIDTWGVDFVYVGKDGTFLGCPRAYRDPYTNSIPEKY
                     FKLIPRKEVYKRTGIQIMNFNSLFQLYAAKLEKSSALEAADKILFMPDALSYMLTGKM
                     VCEYSIASTSQILNPRTRKFDPSLLEVAGINPDILPEITDPGVVIGPLCDALAEESGL
                     GKVPVIAVAGHDTGSAVASVPAENECFAYLSSGTWSLMGIEVREPIINEETFQMNFTN
                     EGGVDGTTRFLKNITGMWLLEQCRKEWKKTGVSYSYPTIVELASSADGFRSLVDPDDP
                     SFANPTNMVKAIVDFCTATGQPVPRNHAQFIRCIFDSLALKYNYVLGKLQQVASFKIE
                     KLHIIGGGAQNRLLNQFTSNAIGLPVVAGPVEATAIGNVMMQAKALGVVGNLQEMRTL
                     ISSSIATEVFLPQDATTWSLAYQKYLNIIKK"
     assembly_gap    4669..4768
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4769..6264)
                     /locus_tag="PLI37_06495"
     CDS             complement(<4769..6264)
                     /locus_tag="PLI37_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOQ58692.1"
                     /translation="MKKHLLILTAVFGIAAMAVSAEKANVVVNADQPGAQISKHIYGH
                     FAEHLGNCIYGGIWVGPESSIPNTDGYRNDIITALKQIQIPNLRWPGGCFADEYHWKD
                     GIGPRASRPKMINTHWGGVVEDNSFGTHEFLNFCELIGTEPYICGNVGSGTIEEMSKW
                     VEYITFDGESPMANLRRENGRDKPWKVRFWGVGNENWGCGGNMTPEHYANLYKNYATY
                     CRNYGDNRLFKIAGGANSADYNWTEVMMRNVGAGQMWGLSLHHYTVPKGWGPGGKGSA
                     TDFTEEEYFTTMEKTLFMDELIGRHVAIMDRYDARKRVALCVDEWGAWYDVEPGTNPG
                     FLYQQNTLRDALLCAANMNIFHKYCDRVRMANIAQMVNVLQSIILTKDDKIVLTPTYH
                     IYDMYKVHQDATLIPVEILSPVYKLGNRSLTALSVSASKDTQGVIHISIVNIDPNNDI
                     EASCLLKGVDAKNVDGVILSSGNLTDKNTFEETQKVVLKEFKKAKINKG"
BASE COUNT         1669 a         1544 c         1429 g         1595 t
ORIGIN      
        1 gacaagaaga agcaacaccg gcagacagca cagtagcatc cggccccttt ttggttccga
       61 aaaacataga ccttaacgtg aatacaagca ttatgaatgc ctatgtgggc aatcaaatcg
      121 ccaccaacct cacaggaagg ctttacgtca aagacgatgc cctggtactc gaagaaatcg
      181 gttttgtcag cgccgccgcc agactcaagc tgacggcttt ataccgctca ccccgcccca
      241 atcacctcta cctgggcctc gactatcata tggttgacat tcagattgac gaactgctaa
      301 gcatgtttcc cgacctggac accattatgc ccatgctgag ctccttcaag ggccagggcg
      361 aatttcatct ggctgccgaa acttacctcg ataaaaacta caacctgaaa aaatccaccc
      421 tgaggggagc ctcctccata cggggcgaca acctggtgtt gatggacagc gaaacctttg
      481 gaaaaatctc caacattctg atgttcaaca aaaagaccga aaacaaaata gacagcctga
      541 gcgccgaatt cacggtattc aaaaacgaag tggatgtgta tccgtttacc atagtgatgg
      601 ataaatacaa agctgtcgtg ggaggccgcc acaacctcga catgagtttc gattatcata
      661 tttctttgct cgaaagcccg ctgcccatgc gcctgggtgt aaacatcaga ggcactatgg
      721 acgaccttaa gatcaggccg gccgcctgta aataccccga aaattaccgc ccggtaagcc
      781 ggagaatagt cgatgcccaa aaattggaat tacgcaaact gattcgggaa accctgatca
      841 agcaaatgga ataaaaacag ggcgtcttta taggacgccc tgtgtacata gatttgccgc
      901 ctgctaaaac agattcggga aaaagacgga aaagaccaaa ataagcaagc ccagaatcag
      961 aacgacaatg gttttggttg atactccttt ccattccttg agcaagattc cccagacatt
     1021 gctgaaggta acgttcaagg ccatcagtat actccacgag aaagccagca taatggcatt
     1081 ctctgccagg aaacttttac cgacggataa accgaaaaat tgagaatacc agagtaatcc
     1141 tgccaaagag cagaacaaca ggttattaac ccatatttta cggttattcg aagtgtagtc
     1201 tttgaagctc ttatttttgg cgttttgcga caaacaataa acggcattgg tgaaaaaacc
     1261 gcctaaagtt accataagag tggtcgggag agtggcaaac aaatcattgg caccaacact
     1321 tttggctgcg tctgcaatgg gttgtcctgc gctgagtccc agagcaaaac aagcactcat
     1381 tacaccggcc agcaatgcca ccaacaaacc tttggtcaaa gcaaaatcct taatggctgc
     1441 tttcttttgc tcttcggtca tgttttgagc tctcaggcca ccggcatagc cgattacggc
     1501 gataccggcc agtgttatac acacacccag taaaagaatc aggccgtctc cgtgaagcaa
     1561 atcctgttta tcaaaaaaca aagccggaat caaggttcca aaagcagagc aagtacctaa
     1621 tgctatggat tgtcccaggg caacgcccag gtaacgcata ctgagtccga aagtcaggcc
     1681 gcccacgccc caaagggcgc caaagcctat ggctttccac gaagcagcgg gatcaacgct
     1741 caacaaagcg cccaatgtcg acccctgagg tacagccagc agggctccca aaaagggaaa
     1801 aaccaaccag gcaaacaccc cctgaaccag ccagaaactt tcccacgacc aattcttaat
     1861 cttgttgatg ggtacataag aactggactg cccgaaactg ccaacggcaa tcaccagcaa
     1921 acctatcagc gtattcatgg tcagcgttta gaagttactt ctttttcgta ttgctgaatc
     1981 tcgggaatga aatcttcacc cacgggaacg ttgttcttca ggcagaaata atcccaaacg
     2041 gcgttccagg gcaagccttt gcattcttcg agcaaagcca gacgttcgaa accttgttcc
     2101 ttgctttcgt attttctcag gatttcgatg ggttcgagca aagcatgcag gaagcatttt
     2161 tgagtggcac ggctaccgat gacataggct ccgatacggt tgatggatcc gtcaaaatag
     2221 tcaagaccga tattaacacg atccaaagca ttggcacgaa caatttcttt ggccaattcg
     2281 agggtgtcgt cgctcataag agtcacgtgg tcggaatccc agcgtatggg acgggaaacg
     2341 tgcaacatga tttcgggggt aaacagcagc aggcaggaaa tcttgtcggc tatgctttcg
     2401 gtcaagtgga agtggccggt atcgagggta acaatctttt gattcttaac tccataaccc
     2461 aggtagaaat cgtgagatcc cactgtatag tattccgcac caataccgaa taatttggat
     2521 tcgatgcaat ccttcatgtt gttgtacttc tgtgcaaaaa tttcatcgag cgattgttcc
     2581 agaatgcgac ggtattccag gcgattgacg gtcatatctt ttgagccgtc gtgaatccaa
     2641 agattcatta cacagggaga accctggtaa acgcccatag cctcggcaat agcccggcaa
     2701 cgtttagtat gttcaatcca gaattcacgg atttcttttt tggggctcga cagggtgagc
     2761 gcttcgcttt tgggatgaga gaaagaggtg gagttgaaat ccaacttcat ttcattttct
     2821 ttggcccatt gcatccagct tgcaaaatgt ttgggttcca gttggtcgcg ttcaacaaat
     2881 ttcccctgaa aatcaccgta aatggcgtga agactcaagc ggtgttttcc gggtataaaa
     2941 gaggtggctt ttttaatatc tgcccgcaat tcatcgatat tcctggcctt gccgggataa
     3001 tttccggtag actgaatacc gccggtcaac tctccgtcgg gattttcaaa tccactgaca
     3061 tcgtcggcct gccagcaatg cagagacaag gatattttct ggagtttatt caaagcggca
     3121 tcgacatcga caccaagtgc tgcataacgt tccctggcaa tttcgtatgc ctgttgaacg
     3181 agtttttctt ttaccataat attcgtttta aaggattaat gtttattttt tgatgatatt
     3241 caaatacttt tgataagcca gcgaccaggt tgtagcatct tgaggcagaa aaacttcggt
     3301 ggcaatagaa gaagatatca gcgtgcgcat ctcctgcaag ttcccaacca ctcccagcgc
     3361 tttagcctgc atcatcacat tacctatggc ggttgcctcc acagggcctg ccactaccgg
     3421 cagtccgatg gcattggacg taaactggtt aagcagccgg ttttgggcac ctcctccgat
     3481 aatgtgcagt ttttctattt tgaaactggc cacttgttgc agtttgccca aaacataatt
     3541 gtacttgagc gccagactgt cgaaaataca acgaatgaat tgtgcgtgat tccggggtac
     3601 aggctgaccg gtagcagtac agaaatcaac aatggccttg accatatttg tcggattggc
     3661 aaaagaggga tcgtcgggat ctaccagcga acggaaacca tcggccgatg atgccagctc
     3721 cacaatagtg ggataggaat aggatactcc cgtctttttc cactccttgc gacattgttc
     3781 cagcagccac ataccggtga tgtttttcag gaaacgggtg gtaccatcta ccccgccttc
     3841 gttggtgaag ttcatttgaa aagtctcttc gttgatgatg ggctctctga cttcaatacc
     3901 catcaacgac caggtgccgg aactcaggta ggcaaaacat tcgttttcgg ccggcaccga
     3961 agccacagca gagcccgtgt cgtgtccggc cacggcaata accggaactt tacccaatcc
     4021 gctttcttcg gccagagcat cgcagagcgg accaatcacc acacccggat cggttatttc
     4081 gggcaatatg tccggattga taccggcgac ttcgagcaga gaaggatcaa acttcctggt
     4141 tcgtggattc agtatctgcg aagtggaggc aatgctgtat tcgcaaacca tttttccggt
     4201 caacatatac gaaagagcat cgggcataaa caaaatcttg tcggctgcct ccagggcaga
     4261 tgatttctcc agtttggcag cataaagctg aaacagactg ttgaaattca taatctgaat
     4321 gcccgtccgt ttgtatactt ctttacgggg gatcagttta aaatactttt cgggaatgga
     4381 attggtgtaa ggatcgcgat aggcccgggg acaacccaga aaagtaccgt ctttgccgac
     4441 atagacaaaa tctacgcccc aggtgtcgat accaatggca tcgatttttt gtccggttgc
     4501 ggcggcagct tttaatcccg ctttgagaga ctcatagagt ccgaaaatat tccagtacga
     4561 cttgccgtgt atgggcataa taagattggg gaaccgggtt aattctttcg tttcgagttt
     4621 acctccgccc agcgttccta aaatggctcg cccgctggtg gctcccagnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc cttgtttatt ttggcctttt tgaactcctt
     4801 gagtacaact ttttgagtct cttcaaaggt gtttttgtcc gtaagattac cggaactcaa
     4861 tattactccg tccacgtttt tggcatccac tcccttcaat aggcaagaag cttcaatatc
     4921 gttgttggga tcaatgttga ctatcgaaat atgtatcaca ccctgagtat ctttcgaagc
     4981 actgacgctc aaagccgtca aagatctgtt ccccagcttg tacaccgggc tcagtatttc
     5041 gacgggaatc aaagtggcgt cctgatgcac tttgtacata tcgtaaatat gataggtagg
     5101 tgtcagcacg atcttatcgt ctttggtcag aataatggac tgcaagacat tcaccatttg
     5161 tgcaatgtta gccatgcgga cacgatcaca gtatttgtga aaaatattca tgttggccgc
     5221 acacagcaaa gcatcgcgca gggtgttttg ctgataaagg aagccgggat tggtgccggg
     5281 ttctacatcg taccaggctc cccattcgtc tacacaaagg gcaactcttt tgcgggcatc
     5341 gtacctgtcc attatggcca cgtgtctgcc gatcagctca tccataaaca acgtcttctc
     5401 catggtggtg aagtattcct cttcggtaaa atcggtggca gatcccttac ctccgggacc
     5461 ccagcctttg gggacagtgt aatgatgtaa tgaaaggccc cacatttgac cggcgccgac
     5521 gtttctcatc atcacttcgg tccaattgta atcggccgaa ttggctccgc cggcaatctt
     5581 gaacaagcgg ttgtctccgt aattacgaca gtaagtggcg taattcttgt ataagttcgc
     5641 gtagtgttca ggggtcatat ttcctccgca accccagttt tcgttaccga caccccagaa
     5701 tctcactttc cagggtttat cgcgtccgtt ctcgcgcctc aggttagcca tgggcgattc
     5761 tccgtcgaaa gtgatgtatt ccacccactt ggacatttct tcgattgttc cgctgcccac
     5821 attaccgcaa atataaggct cggtacctat caattcgcaa aaattcaaaa attcatgggt
     5881 accaaaagag ttgtcttcga ccactccgcc ccagtgggta ttgatcattt tgggccggct
     5941 tgctctgggt ccgataccgt ctttccagtg gtactcatcg gcaaagcaac ctccgggcca
     6001 acgcaggttg ggtatctgaa tttgtttgag agccgtaatt atgtcgttgc gataaccgtc
     6061 ggtgttgggg atggacgatt ccgggcccac ccagatgcca ccgtaaatac aattacccaa
     6121 atgttcagca aaatgtccgt aaatgtgttt gctgatttga gcacccggtt ggtcggcgtt
     6181 tacaaccaca ttggcctttt ctgcagatac agccatcgcg gcaataccga agacagctgt
     6241 caggataaga aggtgttttt tcatagcgat agaattattc agtttgttga taaaaagttt
     6301 tgcattcagc aaagataatt ttttttcttt aaaagcg
//
LOCUS       DAOSTD010000093         6270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_11486_length_6270_cov_14.733065, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000093 DAOSTD010000000
VERSION     DAOSTD010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11486_length_6270_cov_14.733065"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLI37_06500"
     CDS             complement(<1..337)
                     /locus_tag="PLI37_06500"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOQ58693.1"
                     /translation="MCFDTPKVMGVINITPDSFYAGARSSLDDLESRVDEMISEGADI
                     IDIGACSTRPGSKEPDQQEELARLLPALELIRKTRPDFPLSVDTFRAEVARVAVEDYQ
                     VDIINDISAG"
     gene            549..1859
                     /gene="murF"
                     /locus_tag="PLI37_06505"
     CDS             549..1859
                     /gene="murF"
                     /locus_tag="PLI37_06505"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOQ58694.1"
                     /translation="MVSLEQLYPLFLKHSAITTDSRNCPVGSIFFALKGERFNGNDFV
                     PQALEAGCAYAVCDENRHWNDERCLLTEDCLSTLQALAARHRQALKHIPLIALTGTNG
                     KTTTKELTATVLEEKFNVLHTQGNLNNHIGVPLSLLKLTNEHQLAVIEMGANHPGEIR
                     ALALLADPDYGLITNVGKAHLEGFGSPENILATKTELYEYIRRKGGKVFVNLDHPDLV
                     EKSQGIQRIAYSSREGESPVLVSGRPAGNAFFLGVEWTDRRDGKRYLAQSRLVGGYNL
                     ENILAAICIGLYFDVPPADINRALENYQPSNNRSQWRQTANNRLIIDAYNANPGSMKA
                     ALEYFEANISGRRALILGSMKELGDYSRIEHERLIKRIREIKPDQILLIGKEFKDTEA
                     PENTLLFDTTQQCADYLRANPLKDYTVLLKGSRSNKLESLLELL"
     gene            1931..3535
                     /locus_tag="PLI37_06510"
     CDS             1931..3535
                     /locus_tag="PLI37_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOQ58695.1"
                     /translation="MKHPIFALFVALSLLACKQKDNYPYAYLYEGLEFEMPRVEVPSF
                     PNREVSIEDFGAVGDGLTLNTEAFNEAFKSLSSKGGGSLIVPSGVWLTGPIVFQSNVR
                     LHLEKAALILFTQDKSAYPVIKTSYEGLEAWRCQSPISGRNLENIAITGQGAINGSGQ
                     VWRPLKKEKVTEAYWKNVIASGGALKNPKYWLPSQKAVEAELMSPDQRNALTEAEWED
                     FKDVLRPVMVSFVECKNIFLQGVLFENSPSWNIHPLMCENIIIDDVFIRNPSFAQNGD
                     GLDLESCKNVLVVNSTLDVGDDAICIKSGRNEEGRRRGIPTENVIVDNCLVFKGHGGF
                     VVGSEMSGGVKNIAVSNCNFIGTDVGLRFKSTRGRGGVVENIYIRNISMIDMVTEALR
                     FNLFYGGKSASEALADGDAAPAEEVIPPVSEETPVFRNIFIRDVYSHNSRRAMLFNGL
                     PEMNIENIQIENAVFTSQIGAELTESTQIKFKNVGIYPQEGPALILYNVKNLEAENIQ
                     IPEGMKNPVRISGQRNQDIKLPDNLD"
     gene            complement(3580..4581)
                     /locus_tag="PLI37_06515"
     CDS             complement(3580..4581)
                     /locus_tag="PLI37_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOQ58696.1"
                     /translation="MSQRYTRHCINQTDNLYICIDLFNNMTLQQLQYIVAVERHRHFL
                     KAAEECGVTQPTLSAMIQKLEDELDVKIFDRSRQPVEPTEIGLQIIHQAKTVLSNAFQ
                     LRDMVNQQRDGLKGELHMGIIPTIAPYLIPDFISGFVQKYPDIDLSISELQTRQIIEH
                     LKHSELHMAILSTPLEDPDLLEIPLYYESFVAYVSPREELSALDEISYHDMPLKKLWV
                     MQEGHCLRNQVFHFCHDVQKRNNIYEAGSIGTLVSIVDKSGGYTIIPELHIPFLSEEQ
                     AKNVRPLVRPRATREVSIVIRPDFIREKLLNAVADTVKKIIPPALLNERLKKYSLKI"
     gene            4617..5108
                     /locus_tag="PLI37_06520"
     CDS             4617..5108
                     /locus_tag="PLI37_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006849328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOQ58697.1"
                     /translation="MLSEKLHQAINKQINAEFWSANLYLSMSAWFADKGLPGFSHWMK
                     EQFKEEQEHAMRFFNYLVSRGAKVEIGAYAAVPVDWKDAKKVFEDTLHHEQVVTGMIN
                     DLYTLALEEKDYATQSMLKWFIDEQVEEEDTARGILDALCHIKDNSFGLYRLDKEMGE
                     RQG"
     gene            5116..5601
                     /locus_tag="PLI37_06525"
     CDS             5116..5601
                     /locus_tag="PLI37_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOQ58698.1"
                     /translation="MSHRLIPLSGTKEEIYKQLLPQLRCLVEDEDSHIANLANITAAL
                     KSTLGFFWVGFYLVDGPWLVLGPFQGEPACTRIAYGKGVCGKAWELSSALVVDDVDKF
                     EGHIACSAKSRSEIVVPVIKNNKVIAVIDVDSEQLAAFDDVDLHYLTEIARLVAGLPG
                     L"
     gene            complement(5754..>6270)
                     /locus_tag="PLI37_06530"
     CDS             complement(5754..>6270)
                     /locus_tag="PLI37_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOQ58699.1"
                     /translation="LPESEKIIHQQTEIELTAPVANRALSAGDKKAYRPEAFSKVCRI
                     NTADTADLMQYPGIGRYTANRILDYRNRLGGYCSPNQLDEISGIYPENLRMLKQCLET
                     DSSEIRKLKLNHSSLEQLRLHPYLNFYQARALIELRQARGKICSPEDLLFLEEFNEQD
                     LNRLLPYLDCR"
BASE COUNT         1708 a         1531 c         1454 g         1577 t
ORIGIN      
        1 tgccggcgga aatatcgttt ataatatcta cctgatagtc ctctaccgcc acccgggcta
       61 cttctgcacg aaaagtatcc accgacagcg gaaaatcggg tctggtttta cgaatcaatt
      121 ccagggccgg caacaagcgc gccaattctt cctgctgatc gggttcttta ctgcccgggc
      181 gggtggaaca ggctcctata tcaatgatat cagcgccctc gcttatcatc tcatctactc
      241 tggattccag gtcgtcgagg ctgcttctgg caccggcata aaaagaatcg ggcgtaatat
      301 taatcactcc cataaccttg ggagtatcga agcacataag ctttcctctt aagcgtatag
      361 aaggagctac agaaggatga ggacacataa gacgataatt gatttgcaga gcgtttcgag
      421 ccggcctcca caaagccaca aaaatactaa atatattcct taatcctcag caaaatgagc
      481 aaaaaaaccg tacatttgaa ggcctttcgt cgcaaaaatt tactcgttga acagacttat
      541 atgtttttat ggtctctctt gaacaactgt atccattatt tttgaaacat tccgccatca
      601 ccaccgatag ccgaaattgc cctgtgggtt ccattttttt tgccttgaaa ggagaaagat
      661 tcaacggcaa cgattttgta ccacaagcat tggaagccgg ctgtgcctac gccgtttgcg
      721 acgaaaaccg gcattggaac gacgaacgct gtcttttgac agaagactgc ctgagtactt
      781 tacaggctct ggctgccaga caccggcagg cgctgaaaca tattcccctg atcgccctaa
      841 cgggaaccaa cggcaaaacc accaccaaag aacttacggc aaccgtactg gaggaaaaat
      901 tcaatgtcct gcatactcag ggcaacctca ataaccatat cggcgtacct ctgagcctgt
      961 tgaaacttac caacgaacac caactggcag taatcgaaat gggagccaat catccgggag
     1021 aaatccgagc cttagccttg ttggccgatc ccgattacgg acttatcacc aacgtgggca
     1081 aggcacatct cgaaggtttt ggttcccccg aaaacatcct ggcaactaaa accgaactct
     1141 acgaatacat ccgccggaag ggagggaaag tctttgtcaa tcttgatcat ccggatttgg
     1201 ttgaaaaatc acagggcata caacgcatcg cctacagcag ccgcgaggga gaatctcctg
     1261 tactggtaag cggccggccg gctggaaatg ctttctttct gggcgtcgaa tggacagacc
     1321 ggcgcgatgg aaagcgttac ttagcacaaa gtcgtctggt tgggggttat aatctggaaa
     1381 atatacttgc ggccatctgc atcggtttgt attttgacgt tcctccggcc gacatcaacc
     1441 gggccctgga aaattaccaa ccttctaaca atcgttctca atggcggcaa acggcaaaca
     1501 accgcctgat catcgatgcc tacaatgcca accccggcag catgaaagcc gctctcgagt
     1561 atttcgaagc caatatttca ggtcgcagag cccttatttt gggcagcatg aaagaattgg
     1621 gcgactacag ccgcatagag catgagcgac tgataaaacg gattcgtgag atcaaaccgg
     1681 atcaaatctt gcttataggc aaagaattca aagacactga agctcctgaa aatacactat
     1741 tgtttgatac cacacaacaa tgcgccgatt acttaagggc taatccattg aaggactata
     1801 ccgtactgct taaaggctcc agaagcaaca aactcgaaag tctgctggaa ctgctctaag
     1861 aatcgaccaa agacagatag aaaatagaaa attattatcc attatttact cttaactaaa
     1921 aaacaagact atgaaacatc cgatttttgc cttattcgtg gccctgagct tgttggcctg
     1981 caaacaaaaa gacaattatc catacgctta cctctatgag ggcctggaat ttgaaatgcc
     2041 ccgggtggaa gtcccctcat ttcccaaccg tgaagtctcc atagaggatt tcggtgctgt
     2101 gggtgacggt ctgacattaa acaccgaggc tttcaatgaa gctttcaaat cgctgtcttc
     2161 caagggcgga ggcagcctta tcgtaccatc gggtgtttgg ctgaccggcc ccatcgtctt
     2221 ccaatctaac gttcgtctgc acctcgaaaa agctgctctc attctgttta cccaggacaa
     2281 atcggcttat cccgtaatta aaacaagcta cgaaggcttg gaagcctggc gctgccaatc
     2341 gcccatcagc ggaagaaatc tcgagaatat tgccatcacc ggccagggag ccatcaacgg
     2401 ttccggccag gtatggagac cgctgaaaaa agaaaaagta accgaggcct attggaaaaa
     2461 cgtgatcgcc tcgggcggcg cactcaaaaa ccccaaatac tggctgccct cgcaaaaagc
     2521 cgtcgaagcc gaactgatga gccccgatca gcgcaatgcc ctgaccgaag cagaatggga
     2581 agatttcaag gacgttttgc gtccggtaat ggtcagtttt gttgaatgta aaaacatctt
     2641 tttgcaggga gtcttgttcg agaattcccc cagttggaac attcatccct tgatgtgtga
     2701 aaacatcatt atcgatgatg tctttattcg taatccctcc ttcgcccaaa acggagacgg
     2761 tctggatctt gaatcttgca aaaatgtact ggtggttaac tctactctcg acgtgggcga
     2821 cgacgctatt tgtatcaaat cgggacgcaa cgaggaaggc cgccgcagag gcatacctac
     2881 cgaaaatgta attgtagaca attgtttggt tttcaaaggg cacggaggat tcgtagtggg
     2941 tagcgaaatg tcgggcggtg ttaaaaacat cgccgtttcc aattgcaact ttatcggaac
     3001 cgacgtcggc ttgcgattca aaagcaccag aggtcgcggt ggtgtggtcg aaaatatcta
     3061 catccgcaac ataagcatga tcgacatggt aaccgaagct ctgcgtttca atctgtttta
     3121 cggaggtaaa tcagcctccg aagccctggc agacggagac gccgctcccg ccgaagaggt
     3181 gattcctccg gtcagcgaag aaactcctgt gttccggaat attttcatca gggacgttta
     3241 ttcccacaat tcccgcagag ctatgttatt caacggctta cccgaaatga atattgaaaa
     3301 catacaaatc gagaacgctg tgtttacttc gcaaattgga gccgaattga cggagtccac
     3361 ccagattaag ttcaagaacg tgggtatcta tccccaggag ggtccggctc tgattctcta
     3421 caacgtcaag aatctggagg ctgaaaacat tcagatcccc gaaggaatga aaaatcccgt
     3481 gcgcatcagc ggtcagcgga atcaggacat taagcttccc gataacctcg actgatttca
     3541 ttcctgtgcc gatcctgcag gagtctttgt acaaccgcac taaattttta gagaatactt
     3601 tttgagcctt tcgttcaaca aggcgggagg aatgattttt ttgacggtgt cggcaacggc
     3661 attgagcaat ttttcgcgaa taaaatcggg acgtatcacg atagacactt ccctggtggc
     3721 ccggggtctg accaaaggac gaacgttttt ggcttgttcc tcactcaaaa atggtatgtg
     3781 taattccgga atgatggtat agcccccact tttgtcgact atgcttacca gggtgccgat
     3841 gctaccggct tcataaatat tattgcgttt ttgtacgtcg tggcagaagt gaaaaacctg
     3901 attacgcaag caatgacctt cctgcatcac ccagagtttt ttgagaggca tgtcgtggta
     3961 agaaatttca tcgagggcgg aaagctcttc gcggggcgac acataagcca caaaactctc
     4021 gtaataaagc ggtatttcca gcaaatcggg gtcttcgagc ggggtcgata aaatggccat
     4081 gtgcaattcc gaatgcttaa gatgctcaat gatttgcctt gtttgcaact cagagatact
     4141 caggtctatg tcggggtatt tctggacaaa acccgaaata aaatcgggaa tcaggtaagg
     4201 ggcaatggtg ggaataattc ccatatgcaa ctcgcctttt aaaccgtcgc gttgctgatt
     4261 aaccatatct cgcaattgaa aagcattgct caataccgtt tttgcctgat gtatgatttg
     4321 cagaccgatt tcggtaggct caacaggttg tctgctccgg tcgaaaattt ttacatccag
     4381 ctcgtcttca agcttttgaa tcattgcgct caaagtaggc tgagtaacac cacattcttc
     4441 ggcggctttc aaaaaatggc ggtgtcgttc cacagccact atgtactgca attgttgtaa
     4501 agtcatatta ttgaataaat ctatgcaaat atagagatta tctgtttgat ttatacaatg
     4561 ccgggtgtat ctttgcgaca agtttaatcg aataattaac tctaataaat actactatgt
     4621 taagcgaaaa attacaccaa gccatcaaca agcaaatcaa tgccgaattt tggtcagcca
     4681 acctgtatct gtcgatgtct gcctggtttg cagacaaagg attgcccggt ttttcccatt
     4741 ggatgaagga acaattcaag gaagaacagg aacacgccat gcgctttttt aattacctgg
     4801 taagtcgcgg tgccaaagtg gaaatcggtg cttacgctgc agtgcccgta gattggaaag
     4861 acgccaaaaa ggtgtttgaa gacaccctgc accacgaaca agtggttacc ggtatgatca
     4921 acgaccttta tacactggct ttggaagaaa aagattacgc cacccaatcg atgctgaagt
     4981 ggtttatcga cgagcaggtc gaagaagaag acaccgcccg cggcattctg gatgcgctct
     5041 gccacataaa agacaacagc tttggtttgt atcgcctcga caaagaaatg ggcgaacgac
     5101 aaggataaac cattcatgag ccatcgtttg attccccttt ccggaaccaa agaagaaatc
     5161 tacaagcaat tactgccgca attgcggtgt ttggtcgagg acgaggatag tcacatagcc
     5221 aatctggcca atatcacggc cgcactaaag tccaccctgg gttttttctg ggtgggcttt
     5281 taccttgtgg acggtccctg gctggtgctt gggcctttcc agggagagcc ggcttgtact
     5341 cgcattgctt acggcaaagg ggtttgcggt aaagcctggg agcttagttc cgccttggtg
     5401 gtagacgacg tggacaaatt cgaaggacat attgcctgca gcgccaaatc ccgctccgag
     5461 attgttgttc ctgtgattaa aaacaacaag gtaattgccg ttatcgatgt tgacagtgag
     5521 cagcttgcgg cttttgacga tgtcgatctc cactacctga cggaaatcgc ccgactggta
     5581 gccggactgc ccggacttta agctcaaaag agccctacgc catcgagccc tacatcatcc
     5641 aatcctgcgc ccccgagcgc tgcgccatca agacctgcgc catcgaatcc tgcaccattg
     5701 aatcctgcac cggacttttt atctccggaa tgtactattc cggttccggc aacttaccta
     5761 caatcaagat aaggaagcag gcggttcagg tcttgctcgt taaattcttc cagaaaaagt
     5821 aagtcctcag ggctgcatat cttgcccctc gcctgcctga gttcaattaa agccctggcc
     5881 tggtaaaaat tcaaataggg atgcaacctg agttgttcca ggctgctgtg gttcaatttt
     5941 agttttctta tctcgctgct gtctgtttcc aggcattgct ttagcatcct cagattctcg
     6001 ggataaatac ccgatatttc gtccaattga ttcggcgaac aatagccgcc caagcggttt
     6061 ctgtaatcca aaatacgatt tgcagtgtac cgcccgatgc cggggtattg catcaaatcg
     6121 gcagtatcgg cagtattgat tctgcaaact ttggagaagg cctccggcct gtaggccttt
     6181 ttgtctccgg ccgacaaggc tctattcgct accggtgcag ttaattcaat ttccgtttgt
     6241 tgatgaatta ttttttccga ttccggcagt
//
LOCUS       DAOSTD010000094         6241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_11547_length_6241_cov_11.121242, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000094 DAOSTD010000000
VERSION     DAOSTD010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11547_length_6241_cov_11.121242"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLI37_06535"
     CDS             complement(<1..220)
                     /locus_tag="PLI37_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOQ58700.1"
                     /translation="MAKFKKSSKGGSQEINASSMSDVIFMLLFFFMTVTTMKEVEYKV
                     QFQLPEATELTKLEKKSLVTYIYIGPPTI"
     gene            complement(226..798)
                     /locus_tag="PLI37_06540"
     CDS             complement(226..798)
                     /locus_tag="PLI37_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOQ58701.1"
                     /translation="MMANKREVPQPNTSSTADIAFLLLIFFLVTTSMAGDKGLSTRLP
                     PPVDSDVEQKVEERNTLVVLVSQENKIMCQKKEIELWELKDIAKEFIENPYNDIHLPK
                     KVPVTINFFGQVEITKPHVISLQNDRSTKYSKYIEVQNELIAAYNELRDELSLRTFGK
                     PFAELDEERQKAVAEYYPQKISEAEPKEYK"
     gene            complement(822..1289)
                     /locus_tag="PLI37_06545"
     CDS             complement(822..1289)
                     /locus_tag="PLI37_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58702.1"
                     /translation="MSKFINILPKLTLWILMLISVGATVLVFAGGVVDPEAEYKEPVL
                     LDTLLYWIGIMIGIIILITIGFSIAQFGKNLFTDPKKALLSLGSVLLLAAVFVVTYVM
                     SDSSQPLEITGYEGVHNRGVWLSVVTMFIDTIAIVASVAILLMLFGGLFKIKK"
     gene            complement(1317..2135)
                     /locus_tag="PLI37_06550"
     CDS             complement(1317..2135)
                     /locus_tag="PLI37_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOQ58703.1"
                     /translation="MKKLFALLALVGMLTFSTASLAWAQDALQTPDSTLAAPDSTVIE
                     EEEVVVAEETSAIGGVYKALKIKYIEGDAFFMSFISLVLILGLAFAIERIIYLNLSEV
                     DTKALLNKVEQALMVEKDMEKAKEICKNTRGPIASIFYQGLLRMDEGVELAEKSIISY
                     GGVQTGLMERNLPWISLFIALSPSLGFLGTVIGMIQAFDKIQQVGDISPTIVAGGMKV
                     ALITTVYGLISAMILQVFYNYILSKIDSIVNEMEDSSISLLDMLVKIEVTKTKK"
     gene            complement(2200..2292)
                     /locus_tag="PLI37_06555"
     tRNA            complement(2200..2292)
                     /locus_tag="PLI37_06555"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2256..2258),aa:Ser,seq:gga)
     gene            complement(2372..3139)
                     /locus_tag="PLI37_06560"
     CDS             complement(2372..3139)
                     /locus_tag="PLI37_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOQ58704.1"
                     /translation="MIDTHSHLYLPEFQNDIEEVLKRAQAAGIAQCWIPGIDAGSLRT
                     MDRLYDLTPAPGFFRLFAGLHPCEVKDNFQEELSLIRSALLSGKYDGIGEIGLDRHWD
                     LSFFEEQLEAFRVQLDWALELNLPVLIHTRDAFEQMMPLIRSYYHKGLRGIFHSFAGT
                     LEQALELTEEGGFYLGINGSITYKNSALSLFLNKIDLQYIVTETDAPYLSPVPHRGKR
                     NEPAYIPLIVEQLALIYKNPAEEVKKISESNANWLFN"
     gene            complement(3132..3836)
                     /locus_tag="PLI37_06565"
     CDS             complement(3132..3836)
                     /locus_tag="PLI37_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58705.1"
                     /translation="MPYRRLPNTDRARIRALNAATKQGDLVGFLDLAYSYKLKNDALL
                     ILSQIEQAYHEYNINLERQTGSSKSYLTHLKTARLYISHFIQVLNLSVLRNEIKKEHK
                     KLYRLDVNRQSIPDLSTEKALIDWGKNIIEGENERIRRGGAPIYNPSIAKVKVFYDNF
                     VEAKISQKILQQNTSRSLTKLARLRENADAIILDIWNQVEDYFKNLSDEDKREQCSQY
                     GIVYYFRKNEKKGKDD"
     gene            complement(3854..4831)
                     /locus_tag="PLI37_06570"
     CDS             complement(3854..4831)
                     /locus_tag="PLI37_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOQ58706.1"
                     /translation="MKSIQEISALIQNGVRKIDFPDQPLGLYEPLRYVLSMGGKRLRP
                     LLCLSACNLYNGEALSKALPLAIGVELFHNFTLIHDDLMDRSLVRRNKATVHTKWNEN
                     TAVLSGDALQIMAFQHIALAPEDKLKDCLDLFNQTAREVCEGQQLDMNFEMLDNVRKE
                     DYIEMVRLKTAVLLGCSLKLGAIIGGAPANEADLLYDFGIHLGIAFQIRDDLLDVYGD
                     PDIFGKAIGGDIVNNKKTFLWIHSIETADSHTRSLFLNCMKSNYMDREEKIKMVTRFY
                     NRLGARESCEQQMLDHQQSALKALDGLGLDKKHLVTLSELAGKLTYRQV"
     gene            complement(4920..5603)
                     /locus_tag="PLI37_06575"
     CDS             complement(4920..5603)
                     /locus_tag="PLI37_06575"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOQ58707.1"
                     /translation="MQVKKSSRANLDKNSTNNFLMGLVIALGFLFIGFEYSNTEVTVQ
                     DIYTQSGEIEEEIMIEITRQYIPPPPPPPQTVHSDLIQIVEDNTLEEEVEWASIEDFP
                     DEAIVMTYGSGDITEEYDPDGEVFMFVEEMPGFPGGESALMKFINDNLRYPQLALENG
                     ISGRVICTFVIDGRGKVSDIQVIRSVDPNLDREAIRVISMMPDWKPGKQRGKPVRVRY
                     TLPIIFKLQ"
     gene            5774..>6241
                     /locus_tag="PLI37_06580"
     CDS             5774..>6241
                     /locus_tag="PLI37_06580"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOQ58708.1"
                     /translation="MRRRLLVLFMLAAALLRIQAGEGVYRFLTLPVSSYAAGLGGTVL
                     SSPENDLNLSLHNPAALSGEMHNQLALGYMNYVADIHLGTAAYARKINERSTWSAALR
                     YVDYGRIIAAGDNLPEGEFFVKDMAVSGGYAFMLSPRWRAGAGMHLVYSVLEHY"
BASE COUNT         1645 a         1335 c         1406 g         1855 t
ORIGIN      
        1 ttatcgtagg cgggccgatg taaatatagg ttaccaggga tttcttttcc agtttcgtga
       61 gttcggtggc ttcaggaagt tggaattgaa ctttgtattc cacttccttc atcgttgtta
      121 ctgtcatgaa gaagaaaagt aacataaaga taacgtccga catcgaagaa gcattgatct
      181 cctgggaacc acccttgctg gatttcttaa acttagccat gatagttatt tgtattcttt
      241 gggttctgct tcggaaatct tttgaggata atattcagca accgcttttt ggcgttcctc
      301 gtccaattct gcaaaaggtt tcccaaaagt tctgagcgaa agctcgtctc tcaattcgtt
      361 atatgctgca atcaactcgt tttgcacttc gatgtatttg gaatatttgg tgctacggtc
      421 gttttgcagg gaaatgacat gtggtttggt tatttctacc tgaccaaaaa aattaattgt
      481 aacgggaact tttttgggaa ggtgaatatc gttgtaggga ttttcgatga attcctttgc
      541 aatatccttg agttcccata gttcaatttc ttttttctgg cacattattt tgttttcctg
      601 acttaccaaa accaccaaag tattacgttc ttcgaccttt tgctctacgt cgctgtccac
      661 aggcggagga agacgggtag aaagcccctt gtctcccgcc atacttgtgg taaccaaaaa
      721 gaagatcagc aacaggaaag caatgtcggc tgttgaactg gtattcggtt gaggtacctc
      781 tcttttatta gccataataa aactagtata atcagatcag gttatttttt tattttaaat
      841 aaaccaccaa acagcatcag aaggatagca actgaggcaa caatagcaat ggtgtcaata
      901 aacatggtta ccacagaaag ccatacaccc cgattgtgta ctccttcgta gcccgtgatt
      961 tccaaaggct gagaactgtc gctcataacg taagttacaa cgaaaacagc ggccagtagt
     1021 aaaacggatc ccagcgatag caaggctttt ttcggatcgg taaataaatt tttaccaaac
     1081 tgagcaatac tgaagccgat agtgattagt ataattatac ctatcataat tccaatccaa
     1141 tacaacaaag tatccagcaa tacaggctcc ttgtattcgg cttccggatc tacaaccccc
     1201 ccggcaaaga ccagtacagt tgcacctacg gagatcagca tcaatatcca tagggtaagt
     1261 tttggaagta tatttataaa tttagacata gtaatatgaa ataaggggat tgattcttat
     1321 ttttttgtct tggttacctc aatcttaacc agcatgtcga gcagggagat ggatgaatct
     1381 tccatttcgt tgacaatgct gtcaattttg gaaaggatgt agttatagaa cacttgcaga
     1441 atcatagccg aaatcaaacc gtaaacggtt gtgatcaagg caactttcat acctccggca
     1501 acaatggtgg gagaaatatc accaacctgt tggattttat cgaaggcctg gatcataccg
     1561 ataacagtac cgaggaatcc taaggaagga gatagggcaa taaacaaaga aatccaggga
     1621 aggttgcgtt ccattaatcc ggtttgtact ccgccgtacg aaataatgga cttttcagcc
     1681 agttcaacac cttcgtccat gcgaagcagt ccctgataga agatggatgc aataggaccc
     1741 cgggtatttt tgcagatttc cttggctttt tccatgtctt tttcgaccat caaggcttgt
     1801 tccactttat tcaacagtgc cttggtatct acttcagaaa gattcaggta aatgatacgc
     1861 tcaattgcaa aagccaagcc cagaattaaa accaaagaga tgaaactcat aaagaaggcg
     1921 tcaccttcga tgtatttaat cttgagagcc ttgtacaccc ctccgatggc agaagtttcc
     1981 tctgctacta caacttcttc ttcttcaata actgtggagt cgggcgctgc cagggtgctg
     2041 tcgggggttt gaagagcatc ctgagcccaa gcaagcgagg cagtgctgaa cgtaagcata
     2101 cctactagtg ccaaaagtgc aaataacttt ttcattgttc gatgatttaa agattaagtt
     2161 aaaacgtttt aattatctgt ttgttaaata cttgcaattt ggcggagaaa gcgggattcg
     2221 aacccgcgat acgattccac accgtataca cgctttccag gcgtgcctct taaaccactc
     2281 gagcacctct cctcgttcaa caatattgtt gaataaaaac ggcgcaaatt acaaaaaaaa
     2341 aatcagatac aagggctttg cccttggttt tttaattaaa taaccaattg gcattgcttt
     2401 cgctgatttt tttgacttct tctgcaggat ttttgtaaat aagggccagt tgttccacta
     2461 tcaatggaat gtaggcaggc tcgttgcgtt ttcctctgtg aggaacaggg ctcaggtagg
     2521 gagcgtctgt ttccgtgact atgtattgca aatctatctt gttcaaaaac aagcttagcg
     2581 cggaattttt ataggtgata ctgccgttga tacccaagta aaaaccgccc tcttcagtca
     2641 attcgagcgc ttgttccaat gtgccggcaa agctgtgaaa tattcccctg agtcctttat
     2701 ggtaatagga acgaattagg ggcatcatct gttcaaaagc atcgcgggtg tgaataagca
     2761 cgggcaggtt caattccaaa gcccagtcca actgaacacg gaatgcttcg agctgttctt
     2821 cgaaaaagct aagatcccaa tgccgatcaa gacctatttc gccgataccg tcgtatttgc
     2881 cagagagcaa agcactgcga atcaggctga gttcttcttg aaaattgtcc tttacttcgc
     2941 agggatgcag gccggcaaac aacctgaaga aacccggcgc aggcgttaaa tcgtacagac
     3001 gatccatagt cctgaggctg ccggcgtcta tgccgggaat ccagcattgc gcaatgcctg
     3061 ccgcctgtgc tctttttaaa acctcttcaa tatcattctg aaattcaggc aggtaaagat
     3121 gggaatgtgt atcaatcatc tttgcccttt ttttcgtttt tgcgaaaata atacacaatc
     3181 ccgtattggc tgcattgctc acgtttgtct tcgtcgctca gatttttgaa gtaatcttcg
     3241 acctgattcc atatatcgag aataatggcg tctgcgtttt ctctgagacg ggccagctta
     3301 gtaagcgaac gcgaagtatt ttgttggaga attttttgag agattttagc ctcgacaaag
     3361 ttgtcgtaaa acactttgac tttggcgatg gaaggattgt aaatgggagc gcctcctcta
     3421 cggatacgtt cgttttcccc ttctatgatg ttttttcccc agtcgatcag tgctttttcc
     3481 gtgcttaaat cgggaatact ttgccggttg acatcgagac gataaagctt tttatgctct
     3541 tttttgattt cattgcgcaa gacgctcaga ttgagtacct gtataaaatg agaaatgtat
     3601 aatctggctg ttttgagatg agtcaggtag gatttgctgc tgccggtttg cctctccaga
     3661 tttatgttgt attcgtgata ggcctgttca atctgagaca aaatcaacag ggcatcgttt
     3721 ttgagtttgt atgaataagc cagatcaaga aagcctacta aatctccctg tttagtggca
     3781 gcgttcagag cgcgtatccg tgctctgtct gtgttaggca atcttctgta tggcataatc
     3841 ggacaaagtt ttattatact tggcggtagg tcagtttacc ggccagttcg ctcaatgtaa
     3901 ccaagtgttt tttgtcaaga cccaggccat cgagtgcttt aagggcagac tgctggtgat
     3961 ccagcatttg ttgttcacaa ctttctctgg ctcccagccg gttgtagaag cgggtgacca
     4021 tttttatttt ttcctccctg tccatataat tgctcttcat gcaattcaaa aagaggctgc
     4081 gtgtatggct gtcggctgtc tcgatggaat gtatccagag gaaagttttt ttattgttga
     4141 caatgtcacc gcctatggct ttgccgaaaa tgtccggatc tccgtacaca tcgagtaagt
     4201 cgtcccggat ttgaaaggct atgccgaggt ggataccgaa atcgtaaagt aaatcggcct
     4261 cgttggcagg ggctcctcct ataatggcgc ccaattttag gctacacccc agtaagacgg
     4321 ccgttttgag tcgaaccatt tcgatgtaat cctctttgcg gacattgtcg agcatttcaa
     4381 agttcatgtc gagttgttgg ccttcgcaca cttccctcgc tgtttgattg aacagatcca
     4441 ggcaatcttt gagtttgtct tcgggggcca atgcaatgtg ttgaaaagcc ataatttgca
     4501 gggcatcgcc cgaaaggaca gccgtgtttt cgttccattt cgtgtgaacg gtggctttgt
     4561 ttcgcctcac cagggaacgg tccattaaat cgtcgtgtat cagcgtaaaa ttatggaaaa
     4621 gttctacgcc gatcgccaag ggcaacgctt ttgaaagggc ttcaccgttg tataggttgc
     4681 aagccgagag gcatagcagg ggcctcagac gttttccgcc catggagaga acgtatcgca
     4741 agggttcgta taaacccaag ggttggtcag gaaaatcgat tttccggacg ccattttgta
     4801 ttagtgcgct gatttcctga atacttttca ttttttacaa agtaaaaaat aacacacaaa
     4861 atacttaggt tccctttaat gtattttgtg tgttattaat tgtcgattgg taaatcccgc
     4921 tactgtaatt tgaagattat aggcagggta taacgtacac gtacgggctt tcccctttgt
     4981 tttccgggtt tccagtcggg catcatgctg attactctta tggcttctcg gtcaaggtta
     5041 ggatcaactg agcggatcac ttgaatatca gaaacctttc cgcgtccatc gataacaaag
     5101 gtacaaataa cgcgaccgga gatgccgttt tccaacgcaa gctgcgggta tctcaagtta
     5161 tcgttgatga atttcatcag tgctgattct cctccgggga agccgggcat ttcttcaaca
     5221 aacatgaaaa cttcgccatc ggggtcgtat tcttctgtaa tatcaccgct gccgtatgtc
     5281 ataacaatcg cctcgtcagg aaaatcttct atggaagccc attcaacttc ttcttccaga
     5341 gtattgtcct caacaatctg aataaggtca gagtgtacag tttgaggtgg agggggaggc
     5401 ggggggatgt attgcctggt aatttctatc attatctcct cttcgatttc acctgactga
     5461 gtgtaaatgt cctgaaccgt aacttcggtg ttggagtact cgaatccgat gaaaaggaag
     5521 cccaaagcaa tgaccagccc catcaaaaaa ttgttggtgg agtttttgtc gagattggct
     5581 cttgatgatt tttttacttg catggcgttt agacgtaaaa agatatgaat tcagttgacc
     5641 gacaaagata cattattttt taattaaaaa aagaagacaa gcaaaaaaac tcttcaagtg
     5701 ttctcataag tagaattcga ggccgggtta ctatggaaat ttactacctt tggtggtttt
     5761 aaaaccggga attatgcgac gcaggttgct tgtgcttttt atgctcgcgg ccgctctgtt
     5821 aagaatacag gcgggggaag gcgtgtatcg cttcctgacc ttgcccgtgt cttcttatgc
     5881 tgcaggcctg ggaggcacag tgctttcctc tcccgaaaac gatttgaatt taagcttgca
     5941 taatccggcg gccctttccg gcgaaatgca caatcaattg gctttgggtt acatgaatta
     6001 cgtagccgat attcatctcg gcacggcagc ctatgcccgc aagatcaacg aaagaagtac
     6061 ctggtcggcc gctttacgct acgtggatta cgggcgcata atcgctgccg gagacaacct
     6121 gccggagggt gagttttttg ttaaagacat ggccgtgagc ggaggctatg cttttatgct
     6181 ttctccccgt tggagggcag gcgccggcat gcatttggtt tattcggtgc tggaacatta
     6241 t
//
LOCUS       DAOSTD010000095         6089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_11884_length_6089_cov_17.113026, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000095 DAOSTD010000000
VERSION     DAOSTD010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11884_length_6089_cov_17.113026"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2918)
                     /locus_tag="PLI37_06585"
     CDS             complement(<1..2918)
                     /locus_tag="PLI37_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOQ58709.1"
                     /translation="MKQLKNPVRQFNAVRLKSRFFGGFFGFLSGCTLAILLLFSVPFV
                     QNARGQNRVGETLTRDSVAATYSEETSLSDSLDRSALETPTGVSETVLSGENDTIPAL
                     SSDSLQVVPDSLANEEGKAGGGLEGINAVVEYQAEDSLIFDAGGMGRLYKQGQIVYED
                     LNLEADYIQMNMDSSLIYASGIEDSTGTVIGSPVFKDTSGEYISKNLKYNFNTRRGII
                     NQVVTQQGEGYVVSGLTKKMEDDALYMVDGKYTTCDDHDNPHFYLQLSKAKVRPKKNI
                     VAGPAHLVVEDVHLPLFIPFGFFPFTESYSSGIITPTYGDELSRGFYLKDGGYYFAIS
                     DYIDLALTGELYSKGSWGARLRTNYRKRYKFSGALNASYLVSVTSERNLPDYAQSKNL
                     SLTWSHSQDPKANPFRSFSASVNFATSGYSRNDIASYYNPEVFSTNTKSSSVNLTQRF
                     PNFPLVLSASMSVSQRSKDSVLTVSLPNLTASVSRFYPFKRKNPVGRERFYEKISMSY
                     TGSLANSISEVKEYDFFKKSLVRDWRNGMRHSIPVSATFNLLNYINITPSFSYTERWY
                     SSSIDKYYDPGLNQLLTDTLWGFNRVWDYSASVSTSTKLYGFFQPLPKLFGTKVNMIR
                     HVLTPSVSLGYRPDFGEERYGYYKTIDYIDVEGNEKTHTYSRYQNSLYGTPGRGKSGT
                     ISFNVANNLEMKVRNDKDTINLFKKVSLIDNFSFGTSYNLAADSLNWSNISANIRIKF
                     GDLYTLNLSGSFDPYTYILNSAGNPVRVNVTQFEKYGIPGRLVGTGTSFSYSINNDTF
                     KKKDKEGKAKGRGGLSGSGSEPPDLDAGLSGPGDETEAFEEPEVIPDPEAALYQPFKM
                     PWNVSLSYTLRYARANFNKERLEYDMKLMHNLSFNGNLAITDKWRFTLASSYNFNTKQ
                     LSTVNCTVSRDLHCWQMSASFIPVGRFKSYNFSIRVKSGMLQDLKYEQQQNPRDNVVW
                     G"
     gene            complement(3000..3536)
                     /locus_tag="PLI37_06590"
     CDS             complement(3000..3536)
                     /locus_tag="PLI37_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOQ58710.1"
                     /translation="MEALQLLHRYLDPYPGLFDIVYSHGRSVSNKALEIMDLHPEIQA
                     DRKFVEEAALLHDIGVYKTYAPEIACFGSLPYICHGYLGADLLREEGYGRHALVCERH
                     TGAGISLRDIVDKELPLPRRDMLPVSIEEQLICFADKFFSKTELLKERSVAEARHKLE
                     RFGAEGLERFDFWCKSFL"
     gene            3606..>4447
                     /locus_tag="PLI37_06595"
     CDS             3606..>4447
                     /locus_tag="PLI37_06595"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOQ58711.1"
                     /translation="MYAQLSGTPMTKITLFAVCLVTCLCSCFGSCSPGRGKAPASPLR
                     TGADQTELYFPLLQGKRFALLLNQSSLIDRTSLADSLCRSGLRPAFLFAPEHGFRGEA
                     QAGETIQDGVDSLTNLTIYSLYGQQKKPSAELMQKLDLVVFDIQDVGTRFYTYLSTLH
                     YLMEACAESGVELVVLDRPNPNDTIDGPVLHEGYTSFVGMHSIPLLHGCTLGELAMMI
                     NSEGWLPNGLRCELRVIPVAGWRHGQAYSLPVRPSPNLRDQQAVCLYPSLCLFEGSLM
                     SVGRG"
     assembly_gap    4448..4547
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4565..5641
                     /locus_tag="PLI37_06600"
     CDS             4565..5641
                     /locus_tag="PLI37_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOQ58712.1"
                     /translation="MHQNLSGAEKPFTVVIDPGHGGRDPGAVGATAFEKNIVLSVALK
                     LGKLIETNNKDINLIYTRNTDVFVKLEERSRIANKAKANLFISIHTDGVPNKKVSGAS
                     VFTLGQDKLSENIEVAKRENSVILLEENYEQRYEGFNPTSAESYIMFEFLQDAHMDQS
                     IAFASLVQNGFVRKKRTDRGVRQAPYWVLWDTGMPSVLIELGFITNPDEERYLKSEKG
                     QDELVRCIYDAVVEFKNDYQRKSMAKTSEENSDTAGAIAQNTQEAREVQDVQAVQDVP
                     VYKIQLFATSTLLDENNSEFLGLKNIDYYREKDLYKYTYGAFSTEKEAAAERKKIASL
                     FPDAFIVVFKDNIRISLEEARSKP"
     gene            5720..>6089
                     /locus_tag="PLI37_06605"
     CDS             5720..>6089
                     /locus_tag="PLI37_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOQ58713.1"
                     /translation="MKKFKLNKESRIGLTVIIAIALFVWGLNYLKGINLLKQAQRYYV
                     VYNQIDGLVTSAPVMLDGFQVGLVKSIDYQYNSPGHILVELNLEKQLNLPVGTKAVLE
                     PALVGNPNISLILGPGPRLLA"
BASE COUNT         1565 a         1435 c         1486 g         1503 t
ORIGIN      
        1 ccccagacca cattgtcgcg gggattttgt tgctgctcgt acttgaggtc ttgcagcatg
       61 cccgatttaa cccggatgga aaagttgtaa gatttgaaac ggcctacagg aataaaactg
      121 gccgacattt gccagcaatg caggtcgcgc gaaacggtgc agtttaccgt agacaattgc
      181 ttggtattga agttgtaact gctggctaat gtaaatctcc atttatcggt gattgcgagg
      241 ttgccattga aactcaggtt gtgcatcagc ttcatgtcgt actccaagcg ctctttgtta
      301 aagttcgcac gggcataacg caaggtataa ctcaggctga cgttccaagg cattttgaag
      361 ggctgataca gggcagcctc aggatcggga atgacctcgg gttcctcaaa cgcttcggtt
      421 tcgtcgcccg gacccgacag cccggcatct aaatcagggg gttcgcttcc ggaacccgat
      481 aaacctcctc tccccttagc ctttccctct ttgtcttttt tcttgaaagt atcgttgttg
      541 atggagtagg aaaacgatgt cccggtaccg acgagccggc cgggaattcc atatttctcg
      601 aactgggtga cgttgacccg taccggatta ccggcactgt tcagaatata ggtgtaggga
      661 tcgaaagaac cgctcagatt caatgtgtac aaatcgccga acttgatacg gatattggcc
      721 gaaatattgc tccagttaag actgtcggcc gccaggttgt aggaggtgcc gaaagagaaa
      781 ttatcgatca ggctcacctt cttaaataaa ttgatcgtgt ctttgtcgtt tctgactttc
      841 atttcgaggt tgttggccac gttgaagctg atcgttcccg atttacccct gccgggagtg
      901 ccatacaaag aattttggta acgcgaatag gtatgggtct tttcgttgcc ctcgacatcg
      961 atatagtcta tcgttttgta atagccgtat cgttcctctc cgaaatcggg cctgtaaccg
     1021 agcgaaacac tgggggtaag cacgtgccgg atcatattaa ccttagtccc gaacagtttg
     1081 ggcaaaggtt gaaaaaagcc gtataatttg gtggaggtac tgacgctggc agaataatcc
     1141 cagacccggt taaagcccca gagggtgtcg gtaagcaatt gattcagacc cggatcgtag
     1201 tatttgtcga tggaactgga ataccagcgt tcggtatagg aaaacgaagg agtgatattg
     1261 atgtaattca acaaattaaa cgtggctgaa acagggatgc tgtgccgcat accgttgcgc
     1321 cagtcgcgca ccagcgattt tttaaagaaa tcgtattctt tcacttcgct tatgctgttg
     1381 gccagggatc cggtatagct catgctgatc ttttcgtaaa aacgttccct gcccaccgga
     1441 tttttgcgtt tgaagggata gaatcgggaa acactggccg tgagattggg cagcgaaacg
     1501 gtcagcaccg agtctttgct gcgctgtgaa acgctcatgc tggcactcag gacaagcggg
     1561 aaattgggaa aacgctgggt taggttgacg ctggagcttt tggtgttggt cgaaaaaact
     1621 tcgggattgt aatacgaagc gatgtcgttg cggctgtaac cactggtggc aaaattgaca
     1681 ctggccgaaa aactgcgaaa gggattggcc ttgggatcct gcgaatggct ccaggttaga
     1741 ctcaggtttt tggactgggc ataatcgggc aagttccttt cactggtgac actcactaag
     1801 taagaggcgt tgagggcccc ggagaactta taacgtttac ggtaattggt tctgagccgg
     1861 gctccccagg atcctttgga atagagctcg cctgtcagtg ccaaatcgat gtaatcgctg
     1921 atggcaaaat aataaccgcc gtctttgagg taaaatcccc gggaaagctc atcaccgtaa
     1981 gtgggcgtta taatacccga tgagtaggat tcggtaaagg ggaaaaaacc gaaaggaata
     2041 aacaggggca aatgtacgtc ttcgaccacc aaatgggcag gaccggccac aatgttcttt
     2101 ttgggacgca ccttggcttt gctcagttgc agataaaagt gcggattatc gtgatcatcg
     2161 caggtagtat atttgccgtc gaccatgtag agggcatcgt cttccatctt cttagtcagg
     2221 ccgcttacga cgtaaccttc tccctgctgg gtaaccacct ggtttataat gccccgccgg
     2281 gtgttgaaat tgtatttgag gtttttggaa atgtactcgc cgctggtatc tttgaaaacc
     2341 ggacttccta ttacggttcc ggttgaatct tctattccgc tggcatagat caggctgctg
     2401 tccatgttca tttgaatata gtcggcttcg agattgaggt cttcgtaaac aatctgtccc
     2461 tgcttgtaca aacgccccat tcccccggca tcgaaaatca gggaatcttc ggcctggtat
     2521 tctactacgg cattgatgcc ttccaagcct ccgcccgctt ttccctcctc attcgccagg
     2581 ctgtcgggaa cgacctgaag actgtctgaa gaaagcgccg gaatagtatc gttttcgccg
     2641 gagaggacgg tttcgcttac acctgtggga gtttccaggg cagaacggtc aaggctatcg
     2701 gaaagcgaag tttcttcgga ataagtcgct gcaacagagt cccgggtaag cgtttctcca
     2761 acacgattct gtccacgagc attctgaacg aaaggaactg aaaataataa aagtatcgct
     2821 agggtacacc cggataagaa gccaaaaaag cctccgaaga atctgctttt gagccgaacc
     2881 gcgttaaatt gccgaacggg attcttgagc tgcttcaatg tatttaactt ataatcaggt
     2941 tgtacatcca tcatactacc ccagggatca aagggacaaa agtaggtaaa accagcggat
     3001 tagagaaagc tcttgcacca aaaatcgaaa cgttccagcc cttcggcgcc gaatcgctcc
     3061 agtttgtgcc gtgcttctgc gacactgcgt tcttttaaca actcggtttt agaaaaaaac
     3121 ttatcggcaa aacagatcaa ttgctcctcg atactgacag gcaacatatc gcgacggggc
     3181 aagggcaatt ctttgtctac gatgtctcga aggctgatac ctgcacccgt gtgccgttcg
     3241 caaaccaaag catgccgccc gtaaccctcc tctctgagga gatcggctcc caagtaaccg
     3301 tgacaaatat aaggtagaga accgaagcaa gctatctcgg gggcataagt cttgtacaca
     3361 ccgatatcgt gcagcagggc tgcttcttcg acaaactttc tgtcggcctg tatttcggga
     3421 tgtaagtcca ttatttccaa ggccttgttg gagacagacc ggccgtggga ataaactata
     3481 tcaaaaagcc cggggtaagg atcaagatat cgatgtaaca attgcagtgc ttccataaat
     3541 atacggagat tggcgacccg gcttcgggcc ccgggcgctt gcagggcaaa gaaaaagaat
     3601 tatttttgta cgcacaacta tccggcacgc ccatgaccaa aattacgctg tttgcagttt
     3661 gcctcgttac ttgtctttgt tcttgtttcg gttcctgctc ccccggccgg ggaaaagctc
     3721 cggcctcccc tttaagaaca ggcgcagatc aaaccgagct ttatttccca ttgttacaag
     3781 gcaaacgttt tgccctccta ttgaaccaaa gctccctgat cgacaggacc agcttggccg
     3841 acagtctttg ccggtccggc ttgagaccgg cttttttatt tgctcccgaa cacggcttca
     3901 gaggcgaggc ccaagccggc gaaactattc aggacggagt ggacagtctg acgaatctga
     3961 caatctattc actctatggc caacagaaaa agccaagtgc ggaactgatg caaaaactcg
     4021 atttggttgt ttttgatatt caggatgtag gaacccgttt ttatacttac ttgagcaccc
     4081 tgcactacct gatggaagcc tgtgcagaat ccggtgtaga actcgtcgtg ctcgaccggc
     4141 ccaatcccaa cgacaccatc gacgggcctg tgctgcacga aggatatacc tcctttgtgg
     4201 gtatgcattc cattccttta ttacacggtt gcacgctggg cgaactggcc atgatgatca
     4261 actcggaagg ctggctgcca aacggcctgc gctgcgagct tcgggttatt cccgtagcag
     4321 gctggcgtca cggacaagcc tactccctgc ccgtcagacc atcgcccaat cttcgcgacc
     4381 aacaggctgt ttgcctttat ccttcgcttt gtttgttcga aggaagcctg atgagcgtag
     4441 gcagaggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgt tccttgccac
     4561 ttcgttgcat cagaacttat cgggagcaga aaaacctttt acggtagtca tcgatccggg
     4621 tcacgggggc agagacccgg gagccgtcgg agccaccgcc ttcgaaaaaa acatagtgct
     4681 ttcggtagcc ctaaaactgg gaaagctcat agaaaccaac aacaaagaca tcaaccttat
     4741 ttatacccgt aacaccgacg ttttcgttaa actcgaagaa agatccagaa tagccaacaa
     4801 agccaaagcc aatctattta tttcgattca taccgacggt gtgcccaaca aaaaagtctc
     4861 gggagcctct gtatttaccc tgggacagga taaactcagc gaaaacatcg aagtggccaa
     4921 acgcgaaaac agtgttatac tgctcgaaga aaattacgaa caacgttacg aaggtttcaa
     4981 tcctacttcg gcagaatctt acattatgtt cgaatttttg caggatgctc acatggatca
     5041 gagtattgct tttgcttcgc ttgtacaaaa cggttttgta cgtaaaaaac gcactgacag
     5101 gggcgtgcgc caggccccct attgggtact ctgggacacc ggcatgccat cggtactgat
     5161 agagctgggc tttattacca atcccgacga agaacgctac ctgaaaagcg agaaaggaca
     5221 ggatgaattg gtgcgttgta tctacgatgc cgtggttgag ttcaaaaacg attaccaacg
     5281 gaaatcgatg gccaaaacct ctgaggaaaa ttcggataca gcgggagcaa ttgcccagaa
     5341 tacacaagaa gcacgagagg ttcaggatgt tcaagctgtg caggatgttc cggtatacaa
     5401 aatacagctc tttgccacaa gcacattatt agacgaaaac aacagtgaat tcctgggact
     5461 gaagaacatc gattattatc gggaaaagga tttgtataaa tacacctacg gcgctttctc
     5521 tacagaaaag gaagcggccg ccgaacgcaa aaagattgcc tcgctgtttc ccgacgcttt
     5581 catagtcgtg tttaaggaca acatccgtat cagtctggaa gaagccagat caaaaccgta
     5641 atacattttc aagaaagtaa gcggtcttta gctacaaagt taccggctga aaacccacgt
     5701 taaaagtaat aaggttatta tgaagaaatt taaacttaac aaggaaagcc gtatcgggtt
     5761 gaccgtcatt atcgccatcg ccttgtttgt ttgggggctg aattacctca aaggtatcaa
     5821 tctgctcaaa caagctcagc ggtattatgt tgtatacaat caaattgacg gcctggttac
     5881 ctcggcaccg gtgatgctgg acggttttca ggtaggactg gttaaaagca ttgattatca
     5941 gtataactct cccggtcata ttttggttga gctaaacctc gaaaaacaac tcaacttgcc
     6001 cgtgggcact aaggcggttt tggagcctgc cctggtgggc aaccccaaca tcagcctgat
     6061 tctgggtccg ggtcctcggt tgctggccg
//
LOCUS       DAOSTD010000096         6056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_11956_length_6056_cov_15.154474, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000096 DAOSTD010000000
VERSION     DAOSTD010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11956_length_6056_cov_15.154474"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PLI37_06610"
     CDS             complement(<1..612)
                     /locus_tag="PLI37_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ58714.1"
                     /translation="MKYLIIGGVAGGATTAARLRRLDEKSEIIVFERGEYISYANCGL
                     PYYIGGIIKDRDQLFVQTPESFGQRFNLEVRNLSEVLSIDRRKKEVLVKDIRDGSEYR
                     ENYDKLILSPGAEPIKPMLPGINQEGIFTLRNVPDTDKIKQYIEQKQARHALVVGAGF
                     IGLEMAENLHHAGLKVTIVEMAEQVMTPLDYSMAALVHQHLKTK"
     gene            804..1832
                     /locus_tag="PLI37_06615"
     CDS             804..1832
                     /locus_tag="PLI37_06615"
                     /inference="COORDINATES: protein motif:HMM:NF022889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HOQ58715.1"
                     /translation="MRELLFETLMRALMITGFVMLMMVVVEYINVQNKNRWAEKLRRS
                     PLTQILMATILGIIPGCMGAFAVVSLYTHRMIGLAAVVAAMIATSGDEAFVMFALFPG
                     KALLLHGILFIIALLTGWIIYLFCKKEREPAGPQYGFEVHQEESCKCFDRSIILPQLK
                     KITFERSLLLLATLVFLLLLALGVIGHEGWGWEKITLLVGSLFLLFVFLTVPEHFLKE
                     HLYNHIIKKHLLRILLWSWGAFIVLYFVQTEQLLGTLVENNPYLILLAAVLIGIIPES
                     GPHLVFVTLFSQQLIPFSILLASSIVQDGHGMLPVLAESRKDFFLIKGISVLIGLVIG
                     TILLFLGL"
     gene            1968..2267
                     /locus_tag="PLI37_06620"
     CDS             1968..2267
                     /locus_tag="PLI37_06620"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOQ58716.1"
                     /translation="MPRGDKTGPKGQGPMTGRGLGFCKGHDSPGYTENFEDDKDLGRG
                     RGFGGGLGRGRGRGFGAGMGRGRGLGFGGGLGRGRGRGFGRGRGMEPGAEASDSQ"
     gene            complement(2300..3205)
                     /locus_tag="PLI37_06625"
     CDS             complement(2300..3205)
                     /locus_tag="PLI37_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOQ58717.1"
                     /translation="MAITACLLWSTAFVGVKIGLNYMSPLLFAGLRFSIAGILILLFY
                     GKFRAYFQAIKTHFWYILLIAFLQTVLMYGLFYTAMNMVPGSMGAMINGTGPLFAAIV
                     AHLFIKDDKITWRSGGSIMLGIVGIVIINIGRQTGSNAGGLEIIGVLLLIANNLVSGL
                     YNVIVKKSQRNISMLALSSASLLIGGIVLILISIPFETVPANLNFPLEFWLALGWLSF
                     LSAAAFALWFTLLKRPGVKVSYLNSWKFIIPVFGAILSWLILPGEYPDIYSITGVIII
                     GASLLWMNNEALKEMFGKWLAKIKP"
     gene            complement(3254..4243)
                     /gene="trpS"
                     /locus_tag="PLI37_06630"
     CDS             complement(3254..4243)
                     /gene="trpS"
                     /locus_tag="PLI37_06630"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOQ58718.1"
                     /translation="MDTVLSGIRSTGHLHLGNYFGALRNFVRMQHENRCFFFIADYHS
                     LTTHPDPASLHANVKDILCEYLAVGIDPEVATIYVQSDVPETVELYLLLNMHAYMGEL
                     AKTTSFKEKARKQPENVNAGLLTYPTLMAADILQHNADKVPVGKDQEQHLELTRRFAR
                     RFNNLYKVNFFTEPQPYNFDQELVKIPGLDGSRKMGKSEGNCIFLCEDPKLIKKKVMK
                     ALTDAGPTEPHSAMPESIENLFVLLKSVSSPDTVAYFEEQWNNCSIRYGELKKQLAAD
                     IIAFTTPIRERYLDICNNDEYLHKVAVNGAEKARASAAATLREVRHIMGIRPF"
     assembly_gap    4246..4345
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4346..5214)
                     /locus_tag="PLI37_06635"
     CDS             complement(<4346..5214)
                     /locus_tag="PLI37_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HOQ58719.1"
                     /translation="MRHFTSITDLGDLKAAVSEARQIKARPFAWQNLGKNKTLLMIFF
                     NSSLRTRLSTQKAALNLGMNVIVLDVNQGAWKLETERGVVMDGDKSEHLLEAIPVMGS
                     YCDVIGVRSFARFESKADDYSEKILNQFIQYSGKPVFSMEAAIRHPLQSFADLITIEE
                     FKERARPKVVLTWAPHPKALPQAVPNSFAETMNLCDYEFVITHPRGYELSPEFSGKAA
                     IEYDQNKALEGADFVYAKNWAAFTDPNYGKILSQDRSWTIDSKKMALTNNARFMHCLP
                     VRRNMIVSDEVIEG"
     gene            complement(5214..>6056)
                     /locus_tag="PLI37_06640"
     CDS             complement(5214..>6056)
                     /locus_tag="PLI37_06640"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOQ58720.1"
                     /translation="ACILKGGSDAEYSNTAIVQLIRQQLLKHGFPGDAVLLLPPDRRA
                     ADELMEATAYVDLIIPRGSQSLIDHVRSHSLVPVIETGAGICHTYIDSSADLQKAANI
                     VHNAKTRRVSVCNALDCMLLHRSRLQDLPEICLKLQDEQVVIYADSEALAALKGHYPG
                     HLLQPAGEKHYGTEFLDYKMAVKTVKSTEEAIEHIARYSSGHSEAIVSEDSEQAQLFC
                     KKVDAACVYVNVSTAFTDGAQFGLGAEIGISTQKLHARGPMALKEITTYKYLITGNGQ
                     IRSK"
BASE COUNT         1509 a         1311 c         1467 g         1669 t
ORIGIN      
        1 ctttgttttc aggtgctgat gaaccagggc agccatagaa tagtccagag gagtcatcac
       61 ttgttcggcc atttcgacta tggtcacttt aagaccggcg tgatgcaggt tttccgccat
      121 ttctaaacca ataaagccgg ccccgactac caaagcatga cgggcctgtt tttgctcaat
      181 gtattgttta atcttatcgg tgtctggtac gttgcgtaaa gtgaagatac cttcctgatt
      241 gatgccgggc aacataggtt ttatgggttc agcgccgggc gaaagaatga gtttgtcgta
      301 gttttcccta tattcacttc cgtctctaat atctttcacc agaacttctt tcttgcgtct
      361 gtcgatggaa agcacttcgg ataaatttct gacttccaaa ttaaatcgtt ggccaaaaga
      421 ttccggagtc tgtacaaaca gttgatctct gtctttgatg atacctccta tatagtaggg
      481 cagaccgcaa ttggcatacg aaatatattc accacgctca aaaacgatta tttcgctttt
      541 ttcatccagc cgtcttagcc ttgctgctgt agtggctcca cccgccacgc cgccgattat
      601 cagatatttc atagagttta atgtctcaaa gtgtaatagt agaatatttt tacaaagtgc
      661 aaatatataa gaattcttca agctctaatt tgtccttttg aaaatcgttc agtttataca
      721 catcaaacaa ataaaatttg taatacgaat aaatccacac agcagatttt gactaaattt
      781 gggcctcaaa aaatacggga aatatgcggg aactcctttt tgaaacactc atgcgtgcac
      841 ttatgataac gggctttgtc atgctgatga tggtggtcgt tgaatatatt aatgtgcaaa
      901 ataaaaatcg ctgggccgaa aaattacgcc gttctcctct gacgcaaatt ttaatggcaa
      961 ccatcctagg gattataccc ggttgcatgg gtgcttttgc ggtggtgtct ttgtacacgc
     1021 acagaatgat aggcctggct gccgtagtgg cagccatgat tgctaccagc ggcgacgaag
     1081 cctttgtgat gttcgccctt ttccccggca aagctttgtt gttgcatgga atactgttta
     1141 ttatagccct cttgaccggt tggataatct atcttttctg caaaaaagaa agggaaccgg
     1201 cggggcctca atacggcttt gaggtccatc aagaggagag ttgcaaatgc tttgacagga
     1261 gcattatact acctcaactc aagaaaatta cttttgaacg tagtctttta ctgctagcta
     1321 ctcttgtgtt cttgctgctg cttgctttgg gcgtaattgg ccatgaaggc tggggttggg
     1381 agaaaatcac cttgctggtc ggcagtcttt ttctcttgtt tgtttttttg accgtcccgg
     1441 aacattttct caaagaacat ttgtacaacc acatcatcaa gaagcacctt ttacgcattt
     1501 tgctctggtc ttggggggct tttatcgttc tctatttcgt acaaacagaa caactattag
     1561 gcacattggt cgaaaacaat ccctacttga ttttgttagc ggccgtattg ataggcatca
     1621 ttcccgaatc tgggccgcat ttggtttttg ttaccttgtt ctcccagcaa ctgattcctt
     1681 ttagtattct tttggccagt tctatagtgc aggatggtca cggcatgttg ccggtattgg
     1741 ccgaaagccg taaggacttc ttcctgatca agggcatcag tgtactgatt ggtcttgtaa
     1801 ttggaacgat attgctcttc ctggggcttt aatcggtcat ttccttccca taacgaacaa
     1861 taatcttaaa taacattaaa tcttgtaggc caaatgaaca tatgttcata ataataggca
     1921 tatttgtatc agaaaaacaa taagtatcat tttaaaaatt tacaattatg ccaagaggtg
     1981 acaaaacagg tccaaaggga caaggtccca tgaccggaag aggattaggt ttttgcaagg
     2041 ggcacgacag cccgggttat actgaaaatt ttgaagacga caaggatctc ggaagaggtc
     2101 gcggttttgg gggaggctta ggccgcggaa gaggccgtgg gtttggagcc ggtatgggcc
     2161 gtggaagagg ccttggtttt ggaggcggct taggtcgcgg aagaggccgg ggttttggta
     2221 gaggcagagg tatggagccg ggagccgaag cctccgatag ccagtgaaaa ggcaaaagaa
     2281 tgcaatatga ccaatagtct taaggtttaa ttttggccag ccatttgcca aacatttctt
     2341 taagggcttc attgttcatc cagagcagtg aggccccgat tataatcacg cctgtaatgg
     2401 aataaatgtc ggggtattca ccgggtaata ttagccaact gagtatggcc ccaaacaccg
     2461 gaatgatgaa tttccaactg ttcaggtaag atacttttac tccggggcgt ttcagcaaag
     2521 taaaccacaa ggcaaaggct gcagccgaca agaaactcaa ccagccgaga gccagccaaa
     2581 attcaagcgg aaaattcaga ttcgccggca cagtttcgaa gggaatggaa atcagaatca
     2641 acacgatccc tccaatcagc agcgaagcag aactcagtgc gagcatcgaa atatttcgct
     2701 ggcttttctt gactataacg ttgtagagtc cgcttacaag attgttggca atcaagagca
     2761 aaacgcctat tatttcgagt cctcccgcat tgcttccggt ctgtctgcct atattgatga
     2821 ttactatacc tacgattccc agcataatac tgccaccact gcgccaggtt attttatcgt
     2881 ctttaataaa caaatgcgcc acaatggccg caaacaaggg acctgtgccg ttgatcatgg
     2941 ctcccataga gcccggcacc atattcatgg cggtataaaa caagccatac atcaatactg
     3001 tttgcaaaaa ggcaattaac agtatgtacc agaaatgtgt tttaatagct tgaaagtagg
     3061 ccctaaactt gccgtagaac aacaaaatca ggataccggc gatagaaaac ctaagtccgg
     3121 caaacaggag tggactcatg taattaagtc cgattttaac gccgacaaaa gcagtagacc
     3181 aaagcaagca ggctgttata gccatgaatg ccgaactatg aataaaactt ttggtttgtt
     3241 gattcattcg taattaaaaa ggccttatac ccataatgtg ccgcacttcg cgcaaggtgg
     3301 cggcagcgct ggcgcgtgcc ttctcggcac cgtttacagc tactttatgc aggtattcgt
     3361 cgttgttgca tatgtctaaa taacgttcgc gaatgggagt ggtaaaggca atgatgtcgg
     3421 cggccagttg ttttttgagt tcgccgtacc ggatgctgca attgttccat tgctcctcga
     3481 aataggccac tgtgtcgggg ctggaaacgc ttttcagaag aacgaagaga ttttcgatcg
     3541 attcgggcat ggccgaatga ggctctgtgg ggccggcatc ggtcagtgct ttcatcacct
     3601 ttttctttat aagtttagga tcttcgcata aaaaaatgca attgccttcg gatttgccca
     3661 ttttgcgact gccgtccagg cctggtattt ttacgagttc ctgatcgaaa ttataaggtt
     3721 ggggttcagt aaaaaaatta accttgtaca aattattgaa gcgccgggca aaacggcggg
     3781 taagttccag atgttgctcc tgatctttgc ccacgggcac tttgtcggcg ttgtgctgca
     3841 agatgtcggc agccatcaga gtggggtaag tgagcagccc ggcgtttacg ttttcgggtt
     3901 gtttgcgggc tttttccttg aacgaagtcg tcttggccaa ttcgcccata taagcatgca
     3961 tattgagcaa aagatagagt tctacggttt cggggacgtc gctttgcacg taaatagtgg
     4021 caacctccgg atcgatgcct acagccaggt attcacacaa gatgtctttt acgttggcgt
     4081 gcagggaggc gggatccggg tgggtggtaa gagaatgata atctgcaata aaaaagaagc
     4141 aacgattttc gtgttgcatc ctgacaaaat tgcgcaaggc gccaaaataa ttacctaaat
     4201 gcagatgccc cgtagagcgt attccgctta aaactgtatc catttnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnccttc gatcacctcg tcgctgacga tcatgtttcg
     4381 tctcaccggc aggcaatgca taaagcgtgc attgttggtc agggccattt ttttactgtc
     4441 gatggtccag gaacgatcct ggctcagaat tttaccgtaa ttcggatcgg taaaggctgc
     4501 ccagttcttg gcgtaaacaa agtctgctcc ttcgagggct ttgttttgat cgtattctat
     4561 tgcggcttta ccggaaaatt ccggggaaag ctcgtagccc cggggatgcg taatgacgaa
     4621 ttcataatcg cagagattca tggtttcggc aaaactgttg ggaacagcct gaggtagcgc
     4681 tttggggtga ggagcccagg tcaggaccac ctttggacgc gccctttctt tgaattcttc
     4741 gatggtgatg aggtcggcaa agctttgcag gggatggcga atggcggcct ccatgctgaa
     4801 aaccggtttg ccggaatatt gtataaactg attcagtatc ttttcggaat aatcgtcggc
     4861 cttgctttca aagcgggcaa aggaacgtac gccgatgacg tcgcagtagg agcccataac
     4921 cggaatggcc tcaagcaggt gttccgattt atctccgtcc atgacgacac cacgctcagt
     4981 ttcgagtttc caggctccct gattcacatc gagcacaata acattcatac ccaggtttaa
     5041 agcggctttt tgggtgctca gtcgggttct gaggctggaa ttgaaaaaga tcatcaacag
     5101 ggtcttgttt ttgcccaagt tttgccaggc aaaaggccgg gctttgattt gccgggcttc
     5161 gcttactgca gctttgaggt cgcccaggtc ggtaatgctg gtaaaatgtc tcattatttt
     5221 gatcgtattt gtccgttacc ggtaattaaa tatttgtatg tagtgatttc ctttaaggcc
     5281 ataggcccac gggcgtgcag cttttgtgtg ctgatgccga tttcggctcc caagccgaat
     5341 tgtgcgccat cggtaaaggc agtcgataca ttgacataaa cacaggcagc gtctactttt
     5401 ttgcaaaaga gttgggcttg ttcggagtct tcgctgacga tagcctcgct gtgtcccgaa
     5461 gaatagcggg ctatgtgttc gatggcttcc tctgtggatt ttaccgtttt gacggccatt
     5521 ttatagtcca gaaactccgt gccgtaatgc ttttcgccgg ccggttgtaa caaatgtccg
     5581 gggtaatggc ctttgagggc tgccagggcc tcggaatctg cgtaaatcac tacctgctcg
     5641 tcctgcaact taaggcagat ttcaggtagg tcttgcaaac gggagcgatg caacaacatg
     5701 cagtccaggg cattgcagac actcactcgg cgggtttttg cgttatgaac aatgtttgct
     5761 gctttttgca ggtcggccga gctgtcaatg taggtatggc aaataccggc accggtttct
     5821 atgaccggta ccagtgaatg gctgcgtacg tggtcgatca aagactgact gcccctgggg
     5881 atgatcagat ctacataagc ggttgcctcc ataagttcgt cggcagcccg gcggtcggga
     5941 ggtaaaagta agaccgcatc gcccgggaaa ccatgtttga gcaattgttg tcgtatcagt
     6001 tgcacgatgg ccgtattgga atattccgcg tccgagccgc ctttgagtat gcaggc
//
LOCUS       DAOSTD010000097         6050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_11968_length_6050_cov_13.382986, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000097 DAOSTD010000000
VERSION     DAOSTD010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11968_length_6050_cov_13.382986"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            349..3474
                     /locus_tag="PLI37_06645"
     CDS             349..3474
                     /locus_tag="PLI37_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ58721.1"
                     /translation="MKKFSYRCIVLLIFLVAGFAGAAYAQLVRGIVSEADGEPIIGAT
                     VVLTDNTSVGTITGLDGSFSLELPDAQNAVLSVSYVGFQTKEVPVNNQRSLVIVLEVA
                     DISLDEVVFIGYGTVKRRDLTGAVSSIGPKEIVLAPTNNIMEALQGRIAGLDITTPSG
                     AIGSNPSVALRGVRSIYGNNDPLLVIDGVIMPTSTIVEDLVVNTGDNSTNMNVTNYLN
                     QLNPADIESIDVLKDAASTAIYGSAGANGVILITTRKGQAGDAIVNFDSYFSLKGTPL
                     FKHGMQGEEWLQYYKTAYKNANGADLIDMSTLFGGNTYYLDAYNKGKWIDWVDEAVSN
                     GQRATTQKYSLSVSAGGPKSQVYSSLSYTTEQGLMSIESSDQVVFRLNIDQNLFSWAK
                     VGFFTNSTFQMNNTCSPVFQSSVGRLPLGDVYDEYGNLNYYYIGTDAGNGQLSPLADW
                     RKDQYANEQKSVYVQPTAYLQIKPFKDLIFKSQLGGSHANVERGRYYGSQSITQSPHY
                     VGYVAPYAEIYQQNEWSYIWDNILTYDKVFNEDHSVTLTGLSSWNYRQISDLYTGSMG
                     QELDAWLFYRMAAGNAFYANSNYKQTQQMSYAGRANYSYKGKYLATASVRYDGVSWLS
                     EGRKWDYFPSMALAWRISDESFMEDMEDWLSNLKLRLSYGKTGNAGGMTAYSTASGMF
                     KYAQNISVDGPNSPAAGGMTQYTGTYGNAGIGWEKSYTWNIGLDFGFFGNRLEGSFEW
                     YDTRTKDLLYMRAMPVTTGITGWGWTLATWQNLGETMNKGWEFSLNSRNIVRKDFNWT
                     TSFDLAWQKDQIVSLPGGDFKDNKLGWFFEGESINSVYAYKYLGIWQQDEAAEAQTYG
                     CEPGAVKIETVEQDGDGGVHIYNANDMQVIGSYIPRVTAGLNNAFNYKNIDLSVYLMG
                     RFGHLVEYSYYSGSASVTGNQPSGVDYWTPDNTDAYYFAPGLNYNPAASAAHFMDGDF
                     IKVKNVTLGYTLPSSLTKKALINRMRIYATAYNPAVWTKSKQLKGIDPESSSSRYPLY
                     KQFVFGLNLTF"
     gene            3497..5371
                     /locus_tag="PLI37_06650"
     CDS             3497..5371
                     /locus_tag="PLI37_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ58722.1"
                     /translation="MKNNIKKIAFIAVAISLCTTFNSCSLDEEDPGGFTFENLSQTTT
                     GFQTLVNNIYFGLERSFYGNTNKINFMAITEAQTDLWTTPRNMDNTNQQYFWFYAGGS
                     PNTTYTLSFLYSLYDGIGSCNKVLQYVHIPPYNTEAERNAKAAEARFMRAVYYYHAAE
                     QFGGATIVTEKNTSIDQSVYVPVKNSPQEIYDQIIIPDLIYAIEWLPKGDYTAITRPT
                     KKSAIGFLARAYLQATRWVDDKTDYYRNALTYAKMLIDDWEVSGGANYMTYMYPNYGD
                     IFSEAENFTNKEALWKHAYFSDATYNGSSNGAHVLNLSHTLFRCQLNEFPARENNADT
                     YVTWEGSVNGSFMPTQHLLSLFDNGDGTLDPRFYKIFQNDWNANKDFVWTKDFANKFD
                     KDTTAVVGQAIAVGEAAIEFIMPQDADYAAKKAAKHTSKHLIVDYADVYNDAAKSIFM
                     KHSYVNTTPDYKADGSVENFFSYFYPSLTKHNTTKFYPQNVSRLRFGNTSAMHILRMS
                     EMYLIAAEADLKVNNGANALAYLNKVRSRAGAQNLSGTVTIRTILDERGRELCGEWVR
                     YYDLARTGMLDNATYLTETNPSLAAYFNVDYVLRPFDTSTFLPSLNNDSIYQNDGYGY
                     "
     gene            complement(5597..6034)
                     /locus_tag="PLI37_06655"
     CDS             complement(5597..6034)
                     /locus_tag="PLI37_06655"
                     /inference="COORDINATES: protein motif:HMM:NF023021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3244 domain-containing protein"
                     /protein_id="HOQ58723.1"
                     /translation="MNKLVLSLLFLGIAFSAFSQNSDNDEIRLFKKSDPKCYSVPESK
                     PVVQESVTASYNTNNLFISIADFTGAVTVEVLDANNMVVINDTRKVSKQAVLTYSIGK
                     LKPGVYTLVVKASGEYYGEFYKKALDNVVLSRESLILSSLSYQ"
BASE COUNT         1746 a         1279 c         1344 g         1681 t
ORIGIN      
        1 caaatatcga gcttaaaatt gatactgcta agttaaaaat gcataagtag aaaaataatc
       61 agcaaaatcg caaaaagagt attaattttg gggaagattt gagtattgag tttgttgtat
      121 aattgcaata attgacttaa atatgttaat agcctagtat cgtgaaagat tgaggaaatg
      181 aggaaaaaag ccgcctctat ttctctatgt ctgattgata actctattaa aaacttagtg
      241 gaatatttta aagagatgcc ggcaaagtga actgttagtg aggttaaacc ccttgggggt
      301 tataaaagag cttgtttatt attaatcaat ttattaatcc taatccttat gaaaaaattc
      361 agttacagat gtattgtgct tctgattttt ctagtggcag gctttgccgg agccgcctat
      421 gctcaactag ttagagggat tgtatcggag gcagacggtg agcctataat cggggctacc
      481 gtggtgctga cagacaatac ttcggttggg acaataacag ggcttgacgg ttctttttcg
      541 ctcgaattac ccgatgcaca aaatgctgtg ttaagtgttt cttacgttgg tttccaaacc
      601 aaagaagttc cggttaacaa tcaacgttca cttgttattg ttttagaagt ggccgatatt
      661 agcttggatg aagtagtttt tatcggctat ggaacggtaa aacgccgcga tttgaccgga
      721 gcagtttcct cgatcggtcc taaagaaatt gtactggcac ccaccaataa tataatggag
      781 gctttgcagg gacgtattgc cggtttggat attactactc ccagcggagc aatcggtagt
      841 aaccccagtg tcgcgctgcg tggtgtccgc tccatttacg gcaataacga tcccctgttg
      901 gttatagacg gcgtgataat gcctacaagt actattgtgg aagatttagt tgtaaataca
      961 ggcgacaata gtaccaatat gaacgtaacc aactacctga atcaacttaa tcctgcagat
     1021 atcgaaagta tagatgtgct caaagatgcc gcatcgaccg caatatacgg atcggctgga
     1081 gccaatggtg taatacttat aaccaccaga aagggccagg ctggagacgc tattgtcaat
     1141 ttcgattcct atttctccct aaaaggcacc ccgctattta aacacggtat gcagggagaa
     1201 gaatggctgc aatattataa aaccgcctat aaaaacgcca atggagccga cttgatcgat
     1261 atgtccacac tattcggcgg aaatacatat tacctcgatg cctacaataa gggtaagtgg
     1321 attgactggg tggacgaggc tgtgtctaac ggacaaagag ccaccactca aaaatacagt
     1381 ttgtcggttt cggccggtgg tcccaaatcg caggtctaca gttccttgtc ctatactact
     1441 gaacaagggt tgatgagtat tgaaagctcc gatcaggtgg ttttccgttt gaatattgac
     1501 cagaatctgt tcagttgggc caaagtaggc ttctttacca acagtacttt ccaaatgaac
     1561 aatacctgta gcccggtgtt tcagagttca gtaggaagat tgcctttggg tgatgtgtac
     1621 gatgaatacg gtaatttgaa ttactattac atcggtacag acgccggtaa cggacaacta
     1681 tcgcctttgg ccgactggcg caaagatcag tatgccaatg aacaaaagag cgtatatgtt
     1741 caacctacgg cctatcttca gattaagcct ttcaaggatt taatttttaa atcacaattg
     1801 ggcggttctc acgccaatgt agagcgtggc cgctattacg gttcacagag cattacccag
     1861 tcaccgcatt atgtgggcta tgtagcacct tacgccgaaa tctaccagca gaacgagtgg
     1921 tcgtatattt gggataatat cctgacctac gacaaagtat ttaacgaaga tcattcggta
     1981 acactcaccg gcttaagctc ctggaattat cggcagataa gtgacttgta tacgggttcc
     2041 atggggcagg agttggatgc ctggttgttc tataggatgg ctgccgggaa cgccttttat
     2101 gccaatagca actacaagca aacccaacaa atgtcgtatg ccggacgtgc caactattct
     2161 tacaaaggga aatacctcgc gactgcttct gttcgttacg atggtgtatc ctggttgtcg
     2221 gaaggcagaa aatgggatta tttcccctct atggccttgg cctggcgcat cagcgacgaa
     2281 tcgtttatgg aagatatgga agactggctg tctaatctga aattacgtct cagctacggt
     2341 aaaaccggta acgccggtgg aatgacggcc tattcgacag cctccggtat gtttaaatat
     2401 gcgcaaaata tttctgtcga cggtcctaat tctcccgctg ccgggggtat gacccaatac
     2461 accggaactt acggtaatgc cggtataggt tgggaaaaat cctatacttg gaatatcggg
     2521 ttggatttcg gctttttcgg caataggctc gaaggatcgt ttgagtggta tgacacccgc
     2581 accaaagact tgctttatat gcgtgccatg ccggttacta ccggaatcac cggttggggc
     2641 tggaccctgg ccacctggca gaacctgggg gaaacgatga ataagggttg ggaatttagc
     2701 ttgaattccc gtaacattgt tcgcaaagac tttaactgga ccacttcttt cgatctagcc
     2761 tggcaaaaag atcagatcgt gtctttgccc ggaggagatt ttaaagacaa taaactgggt
     2821 tggttctttg agggagagtc tattaactca gtgtatgcat acaagtacct gggtatctgg
     2881 caacaagacg aagctgcaga agctcagacc tatggttgcg aaccgggtgc cgtcaagata
     2941 gaaaccgtag aacaggacgg cgacggcgga gttcatattt acaacgccaa cgatatgcaa
     3001 gtgataggtt cttatattcc cagagttact gccggattaa acaatgcttt taactataaa
     3061 aacatcgatt tgagcgtcta tttgatggga cgtttcggac atctggttga atacagttat
     3121 tacagcggtt cggcttccgt aacaggcaac caaccctcgg gagttgatta ctggacaccc
     3181 gataacaccg acgcgtatta ttttgctccg ggactgaact acaatcctgc cgcatcggcc
     3241 gctcatttta tggacggaga ctttattaag gtcaagaatg tcactttagg ttatactttg
     3301 ccttctagtc tcaccaaaaa ggccctgatc aacaggatga gaatctatgc aacagcgtac
     3361 aatccggctg tttggaccaa gagcaaacag ctcaaaggaa tagatccgga atcttcttct
     3421 tcgcgctatc ctttgtacaa acaatttgtg tttggtctta atcttacatt ttaatcattg
     3481 aaaggagatt acagttatga aaaacaatat taaaaagata gcttttattg ctgtagcaat
     3541 aagcctctgt accactttca actcttgttc acttgacgaa gaggatcccg gcggttttac
     3601 tttcgagaat ttatctcaaa caaccacagg attccaaacg ctggtcaaca atatctattt
     3661 tggtctggaa agaagttttt acggtaatac caacaaaatt aattttatgg ccattaccga
     3721 agcacagacc gatttgtgga caactccgcg caacatggat aatacaaatc aacaatactt
     3781 ttggttttat gcaggcggtt cgcccaacac tacttatacc ttaagctttt tgtattcttt
     3841 gtacgatgga atcggttcct gtaataaggt tttgcaatat gtgcacattc ctccgtacaa
     3901 taccgaagcg gaacgcaatg ccaaggctgc tgaggcccgt tttatgcgtg ctgtctatta
     3961 ttaccatgcc gcagagcaat ttggaggggc tactatagtg acggaaaaaa acaccagcat
     4021 agatcagagc gtgtatgttc cggttaaaaa ttctcctcag gaaatatacg atcaaatcat
     4081 aattccggat ttgatctatg cgatagaatg gctgcctaag ggagattata ctgctattac
     4141 ccggccaaca aaaaagtcgg ccattggctt tttagccaga gcttatttgc aggccacccg
     4201 ctgggtcgat gataaaaccg attattacag aaatgccctg acctatgcta agatgctcat
     4261 agacgactgg gaagtttccg gcggtgccaa ttacatgacc tatatgtatc ccaattacgg
     4321 cgatattttt tcggaagcgg aaaatttcac caacaaagaa gctttgtgga aacatgccta
     4381 tttttcagat gcgacctaca acggtagtag caatggtgct catgtgttga atctaagcca
     4441 taccttattc cgttgccagt taaacgaatt cccggccaga gaaaacaatg ccgatactta
     4501 tgttacctgg gaaggatcgg tcaacggttc ctttatgccg actcagcact tacttagctt
     4561 gtttgacaat ggcgatggca cactcgaccc gcgtttctac aaaatctttc aaaacgattg
     4621 gaatgccaac aaggatttcg tgtggaccaa agactttgcc aataaatttg acaaagacac
     4681 gacagccgtc gttggccaag ctattgccgt aggagaagcc gccatagagt ttatcatgcc
     4741 gcaggacgcc gattatgccg ctaaaaaggc agctaaacac accagcaaac atttaattgt
     4801 ggattatgcc gatgtgtata acgatgcagc caagagcata tttatgaagc acagctacgt
     4861 caacacaaca cccgattata aagccgatgg aagtgttgaa aatttcttct cttattttta
     4921 tccttcgttg accaagcaca ataccactaa attttatccg cagaatgtgt cgagattgcg
     4981 ctttggcaac acctcggcca tgcatatatt gcgtatgtcc gaaatgtatc tgattgctgc
     5041 tgaggccgat ttgaaagtca acaatggagc caatgccctt gcttatttaa ataaagttcg
     5101 cagtcgggcc ggtgctcaga atctgagcgg aaccgtcact atcaggacaa tattggatga
     5161 acgcgggcgc gaattatgcg gtgaatgggt tcgttattac gacctcgccc gtaccggaat
     5221 gttggacaat gctacttatt tgactgaaac caatccttcg ctggccgcct atttcaatgt
     5281 ggactacgtt cttcgtccct tcgataccag cacattcctg ccttcactca acaacgactc
     5341 catttatcag aacgatggat acggttatta acagttgatg agtctaaaga aaaagagggt
     5401 aactctgcgg agttatcctc tttttttacc atttaaccgg catttttgtc cgattttgta
     5461 gtggtttgat acaactaaag ctccctgaat caacaaatat cacccacatt tgaaagattt
     5521 gttgacgcag gaagcttcga gttgcttgat ttatccttaa tttaaaacat cctgaactcc
     5581 ttcaggactc tgccccctat tggtaggata aagaacttaa aatcagggat tctctggaaa
     5641 gaacaacatt atcgagggct tttttgtaaa actctccata atattcacca gaggccttta
     5701 caaccaaggt atacactccc ggtttaagtt tgccgataga ataggtcaga acagcttgtt
     5761 ttgatacctt acgggtatcg ttaattacca ccatattatt ggcgtcaagc acttctaccg
     5821 ttaccgcccc ggtaaaatca gcgatgctga tgaacaggtt gttggtgtta taagaagctg
     5881 tcacagattc ttgtactaca ggcttagatt ccggaacgga gtaacatttg ggatcggatt
     5941 ttttgaagag acgaatctca tcgttatccg aattttgtga aaaagctgaa aacgcaattc
     6001 ccaaaaacaa taatgataat acaagtttgt tcataaggca aaaatttaaa
//
LOCUS       DAOSTD010000098         5959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_12193_length_5959_cov_13.548272, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000098 DAOSTD010000000
VERSION     DAOSTD010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12193_length_5959_cov_13.548272"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2193
                     /locus_tag="PLI37_06660"
     CDS             <1..2193
                     /locus_tag="PLI37_06660"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58724.1"
                     /translation="VPELGRNAEITFENNTVCITGPTQGIVATGTYLGQESEFNFHNN
                     LFLVPHWENDLNLHDTLFSSPRIISCRYGIINASHNLVEGYARWESGQNIDAETGDGA
                     FLALDTMPQYTMADLELSWSDFLNPEGGDYSYMYNIPLATAGSDGGPIGDPRWVMTFA
                     DPKNLTLVSNAEGAVVTPAMGVYESGTEVTISASEVVGFTFQNWQDTLGNVVSTENPY
                     TFNITSDTYLVAYYEELLSRAITIKLNGTNTATYSLSPAREIYYVGDEVTITVNAHYL
                     NDFLGWSDGSMELSRSFVVANDTTIVANFRQYPYMLAWDFCQLTANNTRFSSLEANHY
                     VDEANKGEMNYVTGDTLAADFQTRNNKFTAEGKELHYSALRRTPAANFDNPDYLFVKF
                     STLGYTHITVKSAIASDNCVHLVQKMQYSLNGTDYIDFAVDTLPSAEADWNQVWFPLQ
                     GTLPKEAQNREEVYVRWIADKTSERVKLASLEEVAYEYLYISQIVILGDDGNGGASWR
                     VKPYDYYLAGDVVEVPGIKLTLGGGTNVWSVSDSVFVFDGVTYISALNGTVNPVDDNN
                     KKFSSSGLPPTVGAFYKFEPSRDGTLDVALVVNPNKTSFIIEDNVALSDYNAFSYPVK
                     TYISFKLNLTSGKTYHIFSEGSKMGIMGFVYEIAGGLGKAELSTQIFANNGSLFVKLN
                     KAESIRVYDLLGRTVKSLELREGLNEINGLNRGIYLVRLGSETVKIQL"
     gene            2323..>3563
                     /locus_tag="PLI37_06665"
     CDS             2323..>3563
                     /locus_tag="PLI37_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOQ58725.1"
                     /translation="MKKLYGRLGFFVLLLSMGIFAIKADTIPAFPGAEGYGRYVTGGR
                     GGTVYKVTNLKDDGSEGSLRWAVNKSGPRTIVFDVSGTIYLKSNLNINNGNLTIAGQT
                     APGDGICLRDYTVQVSADNIIIRFMRFRLGDVTATENDAIWGRRRSNIIIDHCSMSWS
                     TDECASFYDNTNFTMQWCVLSESLRESVHGKGTHGYGGIWGGKGASFHHNVLAHHDSR
                     NPRFCGSRYSNRPDLEKVDFRNNVVYNWGSNSGYAGEGGSYNMVNNYYKFGPATKTNV
                     RSRIFQPWADNGQNSQPAGVTGLFYVNGNYMYGNAAVTGDNWQGIHPSSGSINTYRTD
                     TEFDFPFTTTHTAGNAFEKVLAYAGASLVRDTIDRRIMEEIRNGNYTYTGSKGGTKGI
                     IDSQEDVGGWPEYNSGSAPVD"
     assembly_gap    3564..3663
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3852..4649
                     /gene="lptB"
                     /locus_tag="PLI37_06670"
     CDS             3852..4649
                     /gene="lptB"
                     /locus_tag="PLI37_06670"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOQ58726.1"
                     /translation="MPEEKLVLRTENLVKRYHKRTVVDRVSIELHQGEIVGLLGPNGA
                     GKTTTFYMTTGLVVPNEGRIFLGEVDITDYPVYKRAQAGIGYLAQEPSVFRKMSVEDN
                     ILSVLEMTKMTRAEQKEKLESLIAEFHLEKVRKNIGDRLSGGERRRTEIARCLAISPK
                     FIMLDEPFAGVDPIAVDDIQQIVAGLVTKNIGILITDHNVRETLSITDRAYMLFEGKI
                     HFQGSPEELASNEIVRRKYLGVNFELTRKRKPVKKSSVAESNRTAQK"
     gene            complement(4681..5757)
                     /locus_tag="PLI37_06675"
     CDS             complement(4681..5757)
                     /locus_tag="PLI37_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOQ58727.1"
                     /translation="MENNYCVIMSGGIGSRFWPASRSTYPKQFLDFFGTGQSLLQQTY
                     HRFSKIIPPENIFVVSNKLYGKLIREQLPGLSPKQILLEPQRRNTAPCIAFASHHISA
                     VNPKANIVVAPSDHVILKEDEFLEHISEGLTFVKENPVLLTIGIKPNRPETGYGYIQK
                     GHQKIASFTKAKTFTEKPDLELAKVFMESGEFLWNSGIFLWNVQSILKAFEEFLPDIH
                     SSFSAGKQLFNTPKEGEFIEKIFSSCPNISIDYSIMEKAPNVYVQEGGFGWSDLGTWG
                     SLHDLSPKDKDNNVVLKCKALCYESSNNIITLPEGKLAVVQKLDGYIVAESDNVLMIC
                     KKEDEQEIRQYVIDAQLKFGDSFV"
BASE COUNT         1549 a         1371 c         1429 g         1510 t
ORIGIN      
        1 gttcccgaac tgggcaggaa tgctgaaata acctttgaaa acaatacggt ttgtataacc
       61 ggtccaactc aggggatagt agctaccggc acctatttag gacaggaatc ggagtttaat
      121 tttcacaaca acctgttttt agttccccat tgggaaaacg atctgaatct tcacgatact
      181 ctattttcta gccccaggat tatcagctgc cgctacggta tcatcaatgc ttcgcataac
      241 ctggtcgagg gttacgcccg ctgggagtcc ggtcagaata ttgatgccga aaccggcgac
      301 ggcgctttcc ttgctttgga caccatgccc cagtacacca tggccgatct ggaattgtcc
      361 tggtccgatt ttctgaatcc cgaaggcggt gattattcgt atatgtacaa tatccccctg
      421 gccaccgcag gcagcgacgg cggccccata ggcgaccccc gctgggtaat gacttttgcc
      481 gatcccaaaa acctgacgct tgtctccaat gccgaaggag cagtagtgac tccggctatg
      541 ggtgtgtacg aaagcggaac ggaagtgacc atttcggcct ccgaagtggt tgggtttacc
      601 ttccaaaact ggcaggatac tttgggcaat gtcgtctcta cagaaaaccc ctatacgttt
      661 aatattacct ccgacaccta tttggtggcc tattacgaag aattactttc gcgggctatc
      721 actataaaat tgaatggcac caataccgcc acctatagct tatcgccggc aagggagatt
      781 tattacgtag gcgacgaagt aactattacc gtcaacgctc actacctgaa cgacttcttg
      841 ggttggagcg acggctcaat ggaattatcg cgcagctttg tagtggcaaa cgacacaacc
      901 atcgttgcca actttagaca gtatccctac atgctggcct gggacttctg tcaactgaca
      961 gccaacaata ccagattcag ttcgctcgaa gccaatcatt acgtcgatga ggccaacaag
     1021 ggcgaaatga actatgtcac gggcgatacc ctcgctgctg acttccagac caggaacaat
     1081 aaatttaccg ccgaaggcaa agagttgcat tacagcgctc taaggaggac tcccgctgcg
     1141 aacttcgata accccgatta tctcttcgta aaattctcca cgctgggcta tacccatatc
     1201 acggttaagt cggcgattgc ctccgacaac tgcgtgcatt tggtacaaaa aatgcagtat
     1261 tcgctcaacg gcaccgatta cattgatttt gccgtcgaca ccctgccttc ggccgaagcc
     1321 gattggaatc aggtttggtt cccgttgcaa ggcaccctgc ccaaggaagc ccaaaaccgg
     1381 gaagaagttt atgtccgctg gattgccgat aaaaccagtg aaagagttaa attggccagt
     1441 ttagaggaag ttgcttacga atacctctat atttcgcaga ttgtgattct gggtgacgac
     1501 ggtaacggtg gggcttcgtg gagagtaaaa ccctacgatt actacctggc cggcgatgtg
     1561 gttgaagttc cgggtataaa actcaccctg ggtggcggca ccaatgtctg gtctgtcagc
     1621 gactcagtgt ttgttttcga cggggtgacc tatatctcag ccctgaatgg aacggtaaat
     1681 cctgtagacg ataacaacaa gaaattctcg tccagtggtt taccccccac ggttggagcc
     1741 ttctataaat ttgaacccag ccgggatgga accttggatg ttgccttggt cgtcaatccc
     1801 aacaagactt cctttattat cgaagacaat gtggctttgt ccgattataa cgctttcagt
     1861 tatcctgtaa aaacctatat ctctttcaag ctcaatctta cttcgggtaa aacttaccat
     1921 atcttcagtg agggctccaa aatgggcatc atgggctttg tgtacgagat tgcaggaggc
     1981 ctgggcaagg cggaattgtc cacgcagatt tttgccaaca acggcagcct gtttgtaaaa
     2041 ttgaacaaag cagaatccat cagggtttac gaccttttgg gcagaacggt gaaatcgctt
     2101 gaactccgcg agggcctaaa cgagatcaac ggcctgaacc ggggtattta cttggtacgc
     2161 ctcggatcgg aaaccgttaa gattcagctg taaacctaaa gatcagctcg cttgcaaagt
     2221 ttggattagc ggatttttcc tatctttgca agctgaatag actaaagagc aagcctccat
     2281 tcggggaggc ttgcttcttt acgaacataa aacacatatt ccatgaaaaa actatacgga
     2341 agattaggat tttttgtgct gttgcttagt atgggaatct ttgcaataaa ggcagatacc
     2401 attccggctt ttcccggagc cgaaggttat ggccgctacg tcaccggtgg tcggggcggc
     2461 acggtgtaca aggtaaccaa tctcaaggac gacggatcgg agggttccct gcgctgggcc
     2521 gtaaataaaa gcggccccag aaccattgtt tttgacgtat cgggcaccat ttatttaaaa
     2581 agcaacctga atatcaataa cggcaatctg accattgcag ggcagacagc ccccggagat
     2641 ggaatttgtt tgagagatta tacggttcaa gtgagcgccg acaacattat cattcgtttt
     2701 atgcgtttca ggctgggcga tgtgacggcc accgaaaacg atgctatttg ggggcgtcgc
     2761 cgttcaaaca tcattatcga tcattgctcc atgagctgga gcaccgatga atgtgcttcg
     2821 ttttacgata ataccaattt taccatgcag tggtgtgttc tgagtgaaag cctccgggaa
     2881 tctgtccatg gtaagggcac acacggctat ggcggtattt ggggcggaaa aggggccagt
     2941 ttccaccaca atgtgctggc gcatcacgac agccgcaatc cccgtttctg cggcagccgc
     3001 tactccaaca ggcccgacct cgaaaaagtg gatttccgta ataacgttgt ttacaattgg
     3061 ggcagcaaca gcggttacgc gggcgaaggg ggcagctaca atatggtgaa caattactat
     3121 aaattcgggc cggctaccaa aacgaatgtg agaagccgta tttttcagcc ctgggcggat
     3181 aacggacaaa actcccagcc ggcaggtgtt acgggactgt tctatgtaaa cggcaattat
     3241 atgtacggaa atgccgccgt taccggagac aattggcagg gcatacatcc ctccagtgga
     3301 agcataaata cttaccgtac agataccgaa tttgattttc cctttactac tacgcatacc
     3361 gccggaaatg ccttcgaaaa ggtattggcc tatgccggag ccagcctggt aagagacacc
     3421 atcgaccgcc gtattatgga ggagattcgc aatggcaact atacctatac aggctccaaa
     3481 ggcggcacca agggcatcat agattcgcag gaagacgtgg gaggctggcc tgaatacaat
     3541 tcgggatcag cgcccgtaga cagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnccggcag gctcagactc catctggaac agatccaaag caacgaatgt cgtgtagatg
     3721 ctacggggtt atcaccgggt atttacatat tgagggctaa atcggtttcg ggaaaggaag
     3781 ctaccataaa gctcctcagg cattgatata gtattccgat ttttgtttac ttttgcgaca
     3841 aaagctcaat catgcccgaa gaaaaattgg tgctgcgcac cgaaaatctg gttaagaggt
     3901 atcataaacg cactgtggta gacagggtgt ccatcgaatt gcatcaggga gaaattgttg
     3961 gtttgctagg ccccaacggt gcaggtaaga ccaccacttt ttatatgacc acaggcttgg
     4021 tggtgcccaa cgaaggccgt attttcctgg gagaggttga tattaccgat tatcccgtgt
     4081 acaaacgcgc ccaggccggt atcggctacc ttgctcagga accttcggtg tttcgtaaaa
     4141 tgtcggtaga agacaacatc ttgtcggtgt tggagatgac caaaatgacc cgggcagaac
     4201 aaaaagagaa actcgaaagc ctcattgccg aatttcattt ggagaaggtg cgcaaaaaca
     4261 taggcgaccg cctgtccgga ggggaaagac gccggacgga aattgcccgt tgcctggcca
     4321 tcagccccaa attcattatg ctcgatgaac cttttgccgg cgtagatcct atcgcagtgg
     4381 acgatattca gcaaattgtg gccggcctgg taaccaaaaa cattggcatt ctcatcacgg
     4441 accataatgt gcgcgaaacc ctgtccatta cggacagggc ttatatgttg ttcgagggaa
     4501 agatacattt tcagggtagt cccgaagaac tggcttccaa cgaaattgtt cgtcgcaaat
     4561 acttaggagt gaattttgag ctgacccgaa aaagaaaacc ggtcaaaaaa tcttccgtcg
     4621 ctgaatcgaa ccggacagct caaaaataag ttccccaaca tttaaaaacg agttccttat
     4681 ttatacaaag gaatctccga attttagctg ggcgtcgatg acgtactggc gaatttcctg
     4741 ttcgtcttct tttttacata tcatcagcac gttgtccgat tcggctacga tataaccgtc
     4801 gagtttttgt accacggcca gctttccctc gggcagagtg atgatgttgt tactgctctc
     4861 gtaacacaat gctttgcact taagcaccac gttgttgtct ttatccttag gcgaaagatc
     4921 gtgtaaggaa ccccaggtgc caaggtcgga ccagccgaaa ccaccctcct gcacatacac
     4981 attgggggct ttttccataa tgctgtaatc gatggagata ttgggacagg atgagaatat
     5041 tttttcaata aattcgccct ctttgggggt attgaacaat tgttttccgg cactgaaaga
     5101 ggaatgaata tcgggtaaga actcctcgaa tgcttttaaa atactttgca cattccaaag
     5161 aaaaatacct gagttccaca aaaactcccc actttccata aacacttttg ccagttcgag
     5221 gtcgggtttt tcggtaaatg ttttggcttt ggtgaatgag gctatttttt gatgtccttt
     5281 ttgaatgtaa ccgtaaccgg tttccggacg gttaggctta atacctatag tgagcaacac
     5341 cgggttttct ttcacaaatg taagcccctc gctgatgtgt tccagaaatt catcttcttt
     5401 aagaataaca tggtccgaag gagcaactac tatattggct ttgggattga cagcactgat
     5461 gtgatgcgaa gcgaaggcta tgcagggagc agtattcctg cgctgaggtt caagcagaat
     5521 ttgtttggga cttaagccgg gtaattgctc ccgaatgagt tttccatata atttattgga
     5581 aacaacaaaa atgttttcgg gcggaatgat tttactgaaa cggtgatatg tttgttggag
     5641 cagcgattga ccggtaccga aaaaatcgag gaactgtttg ggatatgtac tgcggctggc
     5701 cggccagaag cggcttccga tgcctccgct cataataacg cagtaattgt tttccataga
     5761 gtattattta gttgggtttt tagcaaagat aacaaactta cggtttcccg gtcttgttcg
     5821 cagaagattt cctacccttg gaagcaggct cttttttatg agcattccaa agcgttgccg
     5881 tgatgtcttg taattctttc tcgagccttt cctgttcggc ggcaatttct tcgggcgtag
     5941 agcagtagtc cataatatt
//
LOCUS       DAOSTD010000099         5835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_12530_length_5835_cov_12.712803, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000099 DAOSTD010000000
VERSION     DAOSTD010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12530_length_5835_cov_12.712803"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PLI37_06680"
     CDS             <1..939
                     /locus_tag="PLI37_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ58728.1"
                     /translation="MTLGYRSIPWIWRYCESRVLEGLAEGIGEAQLRRLIMEYRAKQA
                     LLDMGLWTPAFRNLLNANFGGVIKSEWSPYWINSPVWIATPYVKTTNDGNGLLTPEYR
                     TTPGWSGANQIPLVVKSDSIVVNFQPIGENMTCQLCYRTKKGETVYSKPVYAGDCILR
                     IPADKKPANDVVIAVICNTNYIYEGEKTRKAHYDYRLQLGHGIYGTANINRKWYDWTL
                     TMNAIENPFVEELQWSVYPTVIKRGTNISINCDDEVFNSPAQLEITSLGGQKLYQSRL
                     LEKNSSISLPAEVSPGIYMLVIQHSGGRTVQKIVVE"
     gene            1095..4367
                     /locus_tag="PLI37_06685"
     CDS             1095..4367
                     /locus_tag="PLI37_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOQ58729.1"
                     /translation="MRRRTILLGLFIYFGLSMATAQSGSEIVSGKVRDELGPLVMVNV
                     IELDEANRVVSHDVTDINGNFSIRIKSPRNKLRFSYVGYETLTVPIDRKVFDIVMADA
                     MVLQAAEITGRKAITQTGGLSIPERELSMALQSIDMSEFEGLSFTSIDEALQGRIAGL
                     DIVVNSGNLGSGSSMRLRGVSSINANSEPLIVVDDNVQAVDQTDFDVATANEERFAQL
                     LNINVEDIESVTVLKDAAATAIWGSQGANGVILIKTRRGAKGPARLTYSYKFSGTHQP
                     KGLNMLTGDQYTMMLKEAYFNPTLSDAAANIIELNYDPTFSEYQQYNNNTDWREQVIK
                     FGQKHSHYLSLSGGGDKANFRVSGGYDGETGTIIAQKLDRFSTRVALDYFVSERIKIM
                     SNFALTYTNNQRNSDALLNLAYRKMPNLTVFEQDIMGNDTHEYYQVLQSCSNELSDQR
                     NLVNPVASAYLAKNLDRNYSIVPEIILNYNLLGLNPDENQLRYEGRILLNISNAYNDS
                     FYPRELTTTIWSSSAVNRAIASSNKSLTLTHRHSFMFTPVFSNTDHSVMSLLRAEVTD
                     GNSGSQSTTTYGLPSAGIESASAEGMLSGISSGAGQWRSIYYTFSAHYSYKSKYMADF
                     SLRRDGTTKFGPSKRWGYFPAFSLRWNISDEPWMRSLEWLSMLSLRPGWGVVGNQPGA
                     EYLYVSRYTTADTYIDMPAIIPSNIRLANLQWETKTTWNLGLDFGFWNNKLTGDVNIY
                     DQYTSDLLMSSVAIPTSSGYAALSWQNVGSMRNNGWELNFQGNQLVELGKLKLGFNLS
                     FANNKNIITEMDQRVLESLNSDFSRSNGTYLTRIQVKNAFGSIYGFRYKGVYQYSEYS
                     EVEIPGISGPNAPVVRNDEGRVIMEDGEPKPMYFSYGTTAAYEFQGGDAIYEDINYDG
                     NINELDIVYLGSSLPLLNGGFGFRLNYGRLSVNTQFNFRYGNKIVNRARMNAEKMYDN
                     TNQSASVNWRWRMDGDITTIPRALYQSGYNWLGSDRFVEDGSFLRCNYVQLSYQIPAQ
                     QLKPLGINQLSFYVSMNNLFILTKYSGVDPELGYGGYSITFDDSQTPRAKSFTAGITL
                     SF"
     gene            4376..>5835
                     /locus_tag="PLI37_06690"
     CDS             4376..>5835
                     /locus_tag="PLI37_06690"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ58730.1"
                     /translation="MKTKNIIRNFSLGLFLFTLSSCGDWLEITPLNDIVLENFWTDKT
                     DVENVVASCYTALLGSDVVSRMLIWGELRSDNMVQGQGIPDAENEILRANILPTNPYT
                     GWTCFYNVINRCNTVLYYAPMVAQKDPNFNESSLKAIEAEAKALRALCYFYLMRTFRD
                     VPYASEPSVDDNQRYDMAPTPFDELLQILIQDLEGCKDYAIKAYGNKAYNTGRITRDA
                     IHALLADLYLWDQDYDNCIRYCDLVIQSKREQYNELNKDKEELASNEYPLIEAETPGS
                     SVTGNSYNQVFGTGNSTESIFELYFLESPGRLTNTTVSRYYGSVNAQRGYLGAPDFIG
                     RDRNGGSNVFLYGNEVFAKKDVRSREYIGRSSGGVFPITKYAYRSVSNENGVYRSTDF
                     ANWIIYRLSDVMLMKAEALTNMISETAEEDTASSEAAQAKILLSQSFELVRAVYNRSN
                     PEAGRDSLNFTNYSSKDNMNALILAERQRELMFEGKR"
BASE COUNT         1562 a         1365 c         1419 g         1489 t
ORIGIN      
        1 atgactttgg gatacaggag tataccctgg atatggcgct attgcgaatc gcgggtactc
       61 gaaggactgg ccgaaggtat aggcgaagca caacttcgtc gcttaattat ggaataccgt
      121 gccaagcagg ctctcttaga catgggtctc tggaccccgg ctttccggaa tcttctaaac
      181 gccaattttg gcggcgtcat caaatcggaa tggtcgccct attggattaa ttctcctgtc
      241 tggatagcta cgccctatgt aaaaaccacc aacgacggca atggtttgtt aacccccgaa
      301 taccgtacca caccgggatg gtcgggagcc aaccagattc cattggtcgt taagtccgac
      361 tctatagtag tcaatttcca gcccatcggc gaaaacatga cctgtcaact gtgttaccgt
      421 accaaaaagg gagaaaccgt ttacagtaag cctgtttacg ccggggattg catcctgcgt
      481 attccggccg ataaaaagcc ggccaacgac gttgtgattg ccgtaatttg caataccaat
      541 tacatttacg agggagaaaa gactcgtaag gctcattacg attatcgtct gcaactgggg
      601 catggtatct acggaacggc caacatcaat cgtaaatggt acgactggac cctgaccatg
      661 aatgccattg agaatccttt tgttgaagag ttgcaatgga gtgtttatcc cactgtaatc
      721 aagagaggaa caaacatcag cataaactgc gatgatgagg tatttaattc tccggctcaa
      781 ctcgaaatca cgagcctggg aggacagaaa ttgtatcaaa gccgtctgct tgaaaagaat
      841 tcttccataa gcttacctgc cgaagtttca cccggaattt atatgctggt cattcagcat
      901 tcgggaggaa ggacggtaca gaagatcgtg gtagaataga aaaacagact atgcatacac
      961 tcttgtattt gtcgctttgg ggacaataat gtttgataaa tggatttttt tatcatttca
     1021 agcagtgaat gaactatatt tttgtgtgag ttactctaaa tgtaataatc aatgaaaaca
     1081 caactgttat gcttatgcgc agaagaacaa ttttactagg cctttttatt tatttcggcc
     1141 tcagtatggc tacggctcaa tcaggttctg aaattgtcag tggcaaggtg cgcgatgagc
     1201 tgggaccttt ggtaatggtg aacgtcattg agctcgacga ggctaaccgt gtagtatcgc
     1261 acgacgtaac ggatatcaac gggaattttt ccattcgtat caaaagcccc cgaaacaagc
     1321 tcaggttttc ttacgtgggt tacgaaacgc tgacggtacc catagatcgt aaggtttttg
     1381 acatagtaat ggccgatgcc atggtgcttc aggctgccga aataacaggc agaaaggcca
     1441 ttacacagac gggaggtctc agtattcccg agagggaatt gtctatggct ttgcagagca
     1501 tagacatgtc ggaattcgag ggactgagtt ttacctctat cgatgaggcc ttgcagggac
     1561 gcatagcggg tctggatatt gtggtcaatt ccggtaatct gggcagcggt tcttccatgc
     1621 gcctgagggg ggtgtcttcc atcaacgcca attcggaacc tttaattgtg gtggacgaca
     1681 atgtgcaggc cgtcgaccaa accgattttg atgtggctac ggccaacgag gaacgttttg
     1741 ctcaattgct gaacatcaac gtggaagaca tcgagagtgt aacagttttg aaagatgctg
     1801 ccgctaccgc catttggggt tctcagggag ccaacggggt tattttgatt aagactcgca
     1861 ggggcgccaa aggcccggcc cggcttacct attcctacaa gttttcggga acgcatcaac
     1921 ccaaagggct caatatgctg acgggagatc agtacaccat gatgctcaaa gaagcctatt
     1981 tcaacccgac tttgagcgat gcggccgcca atattattga gctgaattac gatcccactt
     2041 tttccgaata tcagcagtac aacaacaata ccgattggcg ggagcaggtc ataaaattcg
     2101 ggcaaaagca cagtcactat ctttccttgt cgggcggagg cgataaagct aatttccgtg
     2161 tttcgggggg gtacgatggt gaaaccggta cgatcattgc ccaaaagctg gatcgttttt
     2221 cgacccgggt ggcgctcgat tattttgtat cggagcgcat caagattatg tccaactttg
     2281 ccctcactta caccaataat cagaggaaca gcgatgcttt gctcaatctg gcttaccgga
     2341 aaatgcccaa cctgactgtt tttgaacagg atataatggg caacgacact catgaatatt
     2401 atcaggtact ccaaagctgt tccaacgaac tcagcgacca gcgcaacctg gtcaatcctg
     2461 tagccagtgc ttatctggcc aaaaacctcg acagaaatta cagcatagtc cccgaaatca
     2521 tattgaatta caatctgctg ggactaaatc cggacgaaaa ccagcttagg tacgaaggac
     2581 gaatattgct gaatatttcc aatgcctaca acgactcttt ttaccccaga gaacttacca
     2641 ctaccatttg gagcagttcg gccgtaaacc gggccatagc ctcgagtaac aaaagtctta
     2701 cgctcactca ccggcacagc tttatgttta ctccggtgtt tagtaatacc gatcattccg
     2761 tgatgtcttt gttgagggcc gaagttaccg acggaaattc cggctcgcag agcaccacca
     2821 cctacggcct gccttcggcc ggcatagaat cggcctctgc cgaaggtatg ttgagcggta
     2881 tttcttcggg tgccggccag tggcggagta tttattacac tttttcggcg cattattctt
     2941 acaagtcgaa atacatggcc gacttttcgt tgcgtcgcga cggtacgact aagttcggtc
     3001 cgagtaaacg ctggggctat ttccctgctt tctcgctgcg gtggaatatc agcgatgaac
     3061 cttggatgcg gagtctcgaa tggctgtcga tgctttcgct tcgtcccggc tggggggtag
     3121 tgggcaatca gcccggtgcc gagtacctgt atgtaagccg ctacaccacg gcagatacct
     3181 acatcgatat gccggccatc attccaagta acattcgttt ggccaacttg caatgggaaa
     3241 ccaaaaccac ctggaactta ggtttggact tcggattttg gaacaacaaa ctgaccggag
     3301 acgtcaatat ttacgatcaa tataccagcg atttgcttat gtcgtcggtg gccataccta
     3361 ccagttcggg ttacgccgct ttgtcgtggc aaaatgtggg cagtatgcgc aacaacggct
     3421 gggagcttaa tttccagggc aaccagcttg ttgaactggg taaactcaaa ttgggtttca
     3481 acctgagttt cgccaacaac aaaaacatca ttacagaaat ggaccagagg gtgctcgaaa
     3541 gcctgaacag cgatttcagc cgcagcaacg gcacctattt aacccgtatt caggtgaaaa
     3601 atgcctttgg ttccatttat ggattcaggt ataagggtgt ttaccaatac agcgaataca
     3661 gcgaagtcga aattccgggc ataagcggac ccaatgctcc ggtagttcgc aacgacgagg
     3721 gacgagtaat tatggaagat ggtgaaccca aacctatgta tttttcctat ggaaccacgg
     3781 ccgcttacga atttcaggga ggagacgcca tctacgaaga tatcaattac gacgggaaca
     3841 tcaacgaact ggatatcgtg tacctgggca gttccctgcc tttgttgaat ggaggtttcg
     3901 gttttcgctt gaattacggc cgcttatctg ttaacacaca gttcaacttc cgttacggca
     3961 acaagattgt aaacagagcc cggatgaatg ccgaaaaaat gtacgacaac actaaccaaa
     4021 gcgcctcggt caactggcgt tggcgcatgg acggagacat cactaccatt cccagggctt
     4081 tgtaccagag tggttacaac tggctgggca gtgatcgttt tgttgaggac ggctctttct
     4141 tgcgctgtaa ctacgtgcaa ttgtcgtatc aaataccggc acaacaactc aaacccctgg
     4201 gtattaacca gctgagtttt tatgtaagca tgaataatct tttcattctg accaagtact
     4261 ccggtgtgga tcccgaattg ggttatggag gatacagcat tacctttgac gattcacaaa
     4321 cgcctagggc taagtctttt acggccggta taacactctc tttctaagta ctgctatgaa
     4381 aactaagaat atcatccgaa atttcagcct gggcctcttt ctctttacct tgagttcctg
     4441 tggagactgg ctcgaaataa cgcccctgaa cgacattgtt ctggaaaatt tctggacaga
     4501 caaaaccgac gtggaaaacg tggttgcatc gtgctatacc gccctgttgg gtagtgatgt
     4561 cgtaagccgt atgttgattt ggggagaact cagatcggac aatatggtac agggacaagg
     4621 tatacccgat gccgaaaacg agatactcag ggccaatata ttgccaacca atccttatac
     4681 cggttggacc tgtttttaca atgtgatcaa ccgttgcaac accgtgttgt attatgcgcc
     4741 gatggtagcc caaaaggatc ccaatttcaa tgagtccagc ctcaaagcca tagaagcgga
     4801 ggctaaagcc cttcgcgccc tctgttattt ctatctgatg cgtactttcc gtgatgtgcc
     4861 ctatgcctca gagccttctg tggacgacaa tcagcgctac gacatggctc cgactccttt
     4921 cgatgagttg ttgcagattt tgattcagga tctggaaggg tgtaaagatt atgccattaa
     4981 ggcctacggt aacaaagcct acaataccgg ccgcattacc cgtgatgcca ttcatgcttt
     5041 gctggccgat ttgtatttat gggaccagga ttacgacaat tgcatcaggt attgcgattt
     5101 ggtcatacaa tccaaacggg agcaatacaa tgaactcaac aaggataaag aagaattggc
     5161 cagcaacgaa taccctctga tcgaagcgga aactcccggc tccagtgtta cgggtaactc
     5221 ttataatcaa gtgtttggaa ccggaaattc caccgaaagt atttttgaac tctatttcct
     5281 ggaatctccc ggtcgtttga ccaatactac agtgtcaaga tattatggta gtgttaatgc
     5341 gcagagaggt tatttgggag ctcccgattt tataggcaga gaccgtaacg gcggcagtaa
     5401 tgtttttttg tacggaaacg aggtttttgc caaaaaagac gtgcgttccc gcgaatacat
     5461 cggccgctca tccggtgggg tgtttcccat taccaaatac gcctaccgaa gcgtgtccaa
     5521 cgaaaacgga gtgtatcggt ctaccgattt tgccaattgg attatttatc gcctcagcga
     5581 cgtgatgctg atgaaagccg aggctcttac aaatatgatt tccgaaacgg ccgaggaaga
     5641 cacggcatcg agcgaagctg cccaggctaa gattttgttg agccaatcgt tcgaactggt
     5701 tcgcgccgtt tacaaccgct ccaatcccga ggcgggcaga gacagcctga atttcaccaa
     5761 ctacagcagc aaggacaata tgaatgccct gatcctggcc gaacgtcagc gtgaactgat
     5821 gttcgaagga aagcg
//
LOCUS       DAOSTD010000100         5732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_12788_length_5732_cov_16.637661, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000100 DAOSTD010000000
VERSION     DAOSTD010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12788_length_5732_cov_16.637661"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1223
                     /locus_tag="PLI37_06695"
     CDS             75..1223
                     /locus_tag="PLI37_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOQ58731.1"
                     /translation="MRRINDIFVANFKTEGMLYIYQRENWPDFTWDNDKLVNLLSDAR
                     NLQGRLIGKMEFLGFEWRDEALLETLTLDVLKSAETEGELLNPGQVRSSIAQRLGMEF
                     AGTTMSDRNVDGMVEMMIDATKNCFKPLTTERLFDWHAGLFPTGRSGIIKITVGEWRK
                     DTSGQMQVVSGAAGREKIHYQAPDASLIEVEMSRFLKWFNEENQTDLVIKAAIAHLWF
                     VTIHPFQDGNGRIARALTDMLLARSDRSTQRFYSMSAQIRIERKEYYEILEKTQKGDL
                     DITEWIKWFLNCLINALKATENVLARVLLKADFWNRHAKTIINERQKNVLNKLLDGFE
                     SKLTSMKWAKMAKCSKDTAIRDINDLINKDILQKGSAGGRSTNYVLKI"
     gene            1295..3511
                     /locus_tag="PLI37_06700"
     CDS             1295..3511
                     /locus_tag="PLI37_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOQ58732.1"
                     /translation="MKALAHLTTFLLLVAYSGISNAIAQNPFKAGFVSLEAEVVSKGN
                     KAEADGNLYEIDTPLLRLMRTNIESPKGTALILAGGGYEILKMRNEGEKTALFLNAEG
                     FDAVVLEYRVSKLKNPNWALADALRAYRLLKTSGKAFGLDGKRLVLVGVSTGGHLAAR
                     LVQKLGDEEQPDDLILISPTNLDETPVNSVFPMVRPPVRPTARLFVSFSANDNKDWIY
                     SAEEYAKTWKGYDGQAMFKLLPDSLYTSQGDANPVDTKLELPGVLKVFLQTPQPGNSA
                     TAPNPAAIPVQGRAKQRYAEKLALAAKEKFDLIMIGNSITHNFEKPEYQPIWNRFFAP
                     RKALNLGTSAYRTENILWDIQNGVLEGQSPKVVVLEIGTNNIDEKNFPTRHTAGQLAG
                     GIEAIVKVLREKLPDTKIIVLRCFPGCYGGPNPTSHRAILERASDMVSKLADGKHIFY
                     CDVNHVFLNMDGSINREAMPDWLHPSPAAAKAWAQAMEPLLCELMGDESRDTEIPANT
                     ALVPAPNLEDNSYNWLGRHKEILSIKDSIDPEIVLIGNSITHLWGGEPPMRWADGKLR
                     EHNGPKSWDSLFHDYRVLNLGFGWDRTQNVLWRLDRGELDGLHPHTVIINIGTNNTGQ
                     SQHGRMNTAPEIVEGIRAICLRVRSKVPDARIVLMAVFPREQTKDHPRRLLINEINAG
                     LESFAREQKITFLNIGSKMLDADGSFLPGLTFDFCHPTEKGYQLWADEIRPIISEP"
     gene            3566..>4273
                     /locus_tag="PLI37_06705"
     CDS             3566..>4273
                     /locus_tag="PLI37_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOQ58733.1"
                     /translation="MTSYFKTVILAVTILLVSCSGAHRPDENIERKHLFNFDWKFHLG
                     DVQDASSESFNDGDWRILDLPHDWSIEATFEKHHPAGNDGGYLPTGIAWYRKTFKAPS
                     QWKDKKTALYFEGVYENSEVFINGISLGVRPYGYASFLYDLTPHLKAGEENVISVRVD
                     NSNQKNCRWYTGSGIYRHVWLIVTDKIHIKHWGVGISTPRVNAKRALVEISTEIKNDT
                     DKTQRIILSTKIKGENSG"
     assembly_gap    4274..4373
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4374..>5732
                     /locus_tag="PLI37_06710"
     CDS             <4374..>5732
                     /locus_tag="PLI37_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ58734.1"
                     /translation="MVKTQMVKGRVAKAQTTETQISIEPGVEKQIVQRIRVKRPLLWS
                     PESPNLYRADLEVLCENRVIDKTTHTFGIRTIEFSSTDGFLLNGEPVILNGGCVHSDN
                     GSLGAAAYNRAEERKVELLKSAGFNAVRTAHNPPSEAFLDACDRLGLLVIDEAFDGWK
                     APKTPYDYSRHFDEWAKRDMQDLVLRDRNHPSVIMWSIGNEVIERTEPEAVETARMLA
                     SSVKEIDPTRPVISAMTTWGQGWEIFDPLMAEHDICGYNYQLWRAPDDHKRVPSRIIV
                     NTESYPGDAFSIWKLVNENNYILGDFVWTAIDYLGEAGIGAYYYPGEDPAEHWQSSRY
                     PWHGAYCGDIDLLGWRKPISHYRSMLYNDNEKLYLAVREPNPEGGEIKLTGWAVWPTW
                     ESWTWPGHEGKNIEVEVYSKYPAVRLYLNDELIGEKQTGETEEFKALFTLPYTPGLIK
                     AVG"
BASE COUNT         1674 a         1185 c         1383 g         1390 t
ORIGIN      
        1 cggcaaacgc tttagagcaa cctgcaacga ataaaactct attcggcgca aataatgcaa
       61 cgaaagtttt ttaaatgcga cgaataaacg atatatttgt tgcaaatttt aaaacggaag
      121 ggatgttata tatatatcag agagagaatt ggcctgattt cacatgggac aacgataagc
      181 ttgttaattt gttgagtgac gcgagaaatc tgcaaggacg attgatcggc aaaatggaat
      241 ttctgggctt tgaatggcgt gatgaagctt tacttgaaac attgacttta gatgtattga
      301 aatcggctga aactgaggga gaactcttaa atcccgggca agtgcgctcc tcaatagctc
      361 aacgattggg aatggaattt gccggtacaa ccatgtcgga taggaacgtg gacggaatgg
      421 ttgagatgat gattgatgcg accaagaatt gttttaagcc cctaacaacc gagagacttt
      481 ttgattggca cgcgggcttg tttccaacgg gaagaagcgg gataattaaa attaccgttg
      541 gcgaatggag aaaagacaca tcaggacaaa tgcaagttgt gtcgggtgca gcgggaagag
      601 aaaaaatcca ttatcaagct cccgatgcct ctctaatcga agtagagatg agtcgtttcc
      661 tgaaatggtt taacgaagaa aatcaaacag atttggtgat aaaagcggcc attgctcacc
      721 tttggtttgt gacaattcat ccctttcaag atggtaacgg acgaattgcg agagccttga
      781 ccgacatgtt gctggcccgg tcggatagga gcacacagcg attctatagt atgtcagctc
      841 aaatcaggat agaaagaaaa gagtactacg agattctcga aaaaacgcaa aaaggagatc
      901 ttgacataac tgaatggata aaatggtttt taaactgttt gattaatgct ttaaaagcaa
      961 ccgaaaatgt ccttgcgcga gttttgttaa aagcggattt ctggaataga cacgcaaaga
     1021 caatcattaa cgagagacaa aagaatgtat taaataaatt actagatgga tttgaaagta
     1081 agctgacttc catgaaatgg gctaagatgg caaaatgctc aaaagacaca gcaatcaggg
     1141 atattaatga cttgataaac aaggatatac tgcaaaaagg ctcagcagga ggaagaagca
     1201 caaactatgt attgaagata taaatgccgt caaacccttg tgcggtattt taagagacag
     1261 atccggagta cattaaaatc ctaaaccaca caatatgaaa gcacttgctc atttgaccac
     1321 ctttttgttg ttagttgcgt attcgggtat ttcgaatgct attgctcaaa atccgttcaa
     1381 agccggtttt gtgtcgctcg aggctgaagt tgtttctaaa ggaaataaag cggaggccga
     1441 tggtaatttg tatgaaatcg atacgccttt gctgcgtctg atgcgcacga acattgagtc
     1501 tcctaaaggt acggcattaa ttcttgcggg aggcgggtat gagatattga aaatgaggaa
     1561 tgaaggcgag aagacggctt tgtttctgaa tgcggaaggc tttgatgctg tcgtacttga
     1621 atatcgcgtt tccaagctga agaaccctaa ttgggctttg gccgatgcgc ttcgggcata
     1681 caggcttctt aaaacaagtg gaaaggcatt tggcctggac ggcaagcgtt tggtgcttgt
     1741 gggcgtttcc accggaggtc atcttgccgc acggctggtg cagaaactgg gcgatgaaga
     1801 acagcccgac gatctgattc tgatcagtcc gactaattta gatgaaacgc cggtaaatag
     1861 tgtcttcccg atggtcagac ctcctgttcg gccaacggca aggctttttg tttccttttc
     1921 tgccaacgat aacaaagatt ggatttacag cgccgaagaa tatgcaaaaa cctggaaggg
     1981 atacgatggc caagcaatgt tcaaattatt gcccgacagc ttgtatactt cgcagggaga
     2041 tgccaaccct gtagatacaa aacttgaatt gcccggcgtt ttaaaggtgt ttctccaaac
     2101 accacaaccc ggcaactcag caactgctcc caatccggcg gctattcccg ttcagggacg
     2161 tgcaaagcag cgttatgcag aaaagctggc cctggctgca aaagaaaagt ttgacctcat
     2221 tatgattggg aattccatca cccataattt tgagaaaccg gaatatcagc cgatatggaa
     2281 ccggtttttt gctccgcgca aagctttgaa cctgggaaca agtgcttacc ggaccgaaaa
     2341 catcctttgg gatatacaaa acggtgtgct cgaaggtcaa tcgcctaaag tagttgtatt
     2401 ggaaatcggc accaataata ttgatgaaaa aaatttcccg acgcgtcata cggcgggtca
     2461 acttgcgggc ggcatcgagg ccattgttaa agtgctgcgc gaaaagctgc cggatactaa
     2521 gataattgtc ctgcgttgtt tccccggttg ctatggcgga cccaatccga cctctcaccg
     2581 ggcaatcctc gaacgggctt cggatatggt gtcgaagctt gccgatggga agcatatttt
     2641 ctattgcgat gtgaaccatg tgtttcttaa tatggatggc tccatcaacc gtgaagcgat
     2701 gcccgattgg cttcatccca gccctgctgc cgccaaagcc tgggctcagg ccatggagcc
     2761 tcttctatgc gagcttatgg gcgacgaatc gcgcgatacg gaaattcctg ccaatacagc
     2821 ccttgtgccc gctcccaacc tcgaagacaa cagctacaat tggttgggac gccacaaaga
     2881 aattcttagt atcaaagatt ccatcgatcc cgaaattgta cttatcggca attccattac
     2941 tcatttatgg ggaggagagc ccccgatgcg atgggccgat gggaaattgc gcgagcataa
     3001 cggaccaaag tcctgggatt cgttgtttca tgattaccga gtgttgaatc tcggttttgg
     3061 ctgggaccgc acgcaaaatg ttctttggag gctcgaccgg ggagagctcg acgggcttca
     3121 tcctcacaca gttattatta atattggcac taataatacg gggcagagcc aacacgggcg
     3181 aatgaatacg gctcccgaaa ttgtcgaggg catacgggcc atttgtttgc gtgtccgatc
     3241 caaagttccc gatgcaagaa ttgtgctgat ggccgttttc ccgcgcgaac aaaccaagga
     3301 tcatccgcgg cgtttattga taaacgaaat caatgcaggg cttgaatctt ttgccaggga
     3361 acagaaaatc actttcctga acatagggtc gaaaatgctc gatgccgacg gaagctttct
     3421 gcccggcctg acttttgatt tctgccatcc cacagaaaaa ggttatcaac tatgggccga
     3481 cgaaattcgt ccgatcattt ctgaacctta atacaaaact ttcgggcgct tcattccgca
     3541 taaataacta taaataaaac ccaccatgac ctcctatttt aaaaccgtta ttctggccgt
     3601 aaccatcctg ttggtaagtt gttccggtgc tcatcggccg gatgaaaata ttgaacgcaa
     3661 gcacttgttc aatttcgact ggaaatttca tttgggcgat gttcaggacg cttcgtcgga
     3721 aagttttaac gacggcgatt ggagaatcct cgatttacct cacgattgga gcattgaagc
     3781 aactttcgaa aagcatcatc ctgcagggaa cgacggaggc tatttaccaa ccgggatagc
     3841 ctggtatcgc aaaacgttta aagcaccgtc ccagtggaaa gacaaaaaaa cagcccttta
     3901 tttcgaagga gtttacgaaa attcggaagt gtttatcaat ggtatatcct tgggtgtgcg
     3961 gccatacgga tatgcttctt ttctctacga tttaacacct cacctcaaag cgggtgagga
     4021 aaatgttatt tctgtccgcg tggataattc caatcagaaa aactgccgtt ggtacaccgg
     4081 ttcaggtatc tacaggcatg tttggcttat agtgacggat aaaattcaca tcaaacactg
     4141 gggagtgggt atcagcactc cgcgggtaaa tgccaaaaga gcccttgttg aaataagcac
     4201 agaaataaaa aacgatacgg acaaaactca acgtataatc ctttcaacca aaataaaagg
     4261 cgaaaacagc gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatggtca
     4381 aaactcaaat ggttaaaggc cgggtggcca aagctcaaac gaccgaaact caaatctcaa
     4441 ttgaaccggg tgttgaaaaa caaatagtac agagaataag agtaaagaga cctttgctat
     4501 ggtcgccgga atctccaaat ttataccggg ccgacctgga agtattatgt gaaaaccggg
     4561 tcatcgacaa aacaacacat acattcggaa taagaaccat agaattcagt agtaccgatg
     4621 gatttttact taatggagaa ccggtgattt taaacggagg ttgtgttcat agcgataatg
     4681 gaagtttagg cgctgctgcg tataatcggg cggaagaacg gaaagtggag ttgctgaaat
     4741 cggccggctt caacgctgtg cgcacagctc acaatcctcc ctcggaagca tttctcgacg
     4801 cttgcgacag gttggggctt ttggtgatag acgaagcctt cgacggctgg aaagctccga
     4861 aaacccccta cgattattca agacatttcg atgaatgggc taagcgggat atgcaggatt
     4921 tagtgttgcg agacaggaac catccatccg taattatgtg gagtataggg aacgaagtaa
     4981 tcgaacgaac agaacccgaa gctgtagaaa cggcaagaat gttggcatcg agcgtaaagg
     5041 aaatagatcc aacccgtcct gtaatctctg ccatgaccac ctgggggcag ggctgggaaa
     5101 tttttgatcc gctgatggcc gaacacgata tttgcggata taactaccaa ctttggcgtg
     5161 ctcctgacga ccacaaaaga gttccttcga ggattatcgt aaatacggaa tcctatcccg
     5221 gagatgcttt ttcgatttgg aaattggtaa acgagaacaa ttacattctg ggcgattttg
     5281 tttggactgc catagattat ctgggtgaag caggtatagg agcatattat tatcccggtg
     5341 aagatcccgc cgaacattgg caaagcagtc gttacccctg gcatggagcc tattgcggag
     5401 atattgactt attggggtgg agaaagccta tttcgcatta cagaagtatg ttgtacaacg
     5461 acaatgaaaa actatacctg gccgtaagag aacccaatcc cgaaggcgga gagatcaaac
     5521 taaccggatg ggctgtttgg ccgacatggg aaagctggac atggccgggg catgaaggaa
     5581 aaaacatcga agtggaagtg tattcgaaat acccggctgt ccgcctttat ttaaacgatg
     5641 aacttattgg agaaaaacaa accggagaaa cagaggaatt caaagcccta ttcactcttc
     5701 cttacactcc cggcttaatt aaggccgttg ga
//
LOCUS       DAOSTD010000101         5590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_13227_length_5590_cov_12.634508, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000101 DAOSTD010000000
VERSION     DAOSTD010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13227_length_5590_cov_12.634508"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PLI37_06715"
     CDS             complement(<1..745)
                     /locus_tag="PLI37_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HOQ58735.1"
                     /translation="MKRREVKFSLMFRDMWQSSGKYVPRVDQLVKIAPHIVKMGCFAR
                     VETNGGGFEQVNLLFGENPNKAVREWTKPFREAGIQTHMLDRGLNGIRMSPVPADVRK
                     LFYKVKKAQGTDIARTFCGLNDLRNIAPSITYAKEAGMISQCSLCITHSPVHTVEYYT
                     NKALELIKMGADEICIKDMAGIGRPVSLGKVVENIKKAHPEIPVSYHGHAGPGFNVAS
                     ILEVCRGGCDYIDVGMEPLSWGTGHPDVIT"
     gene            complement(749..1000)
                     /locus_tag="PLI37_06720"
     CDS             complement(749..1000)
                     /locus_tag="PLI37_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HOQ58736.1"
                     /translation="MENLSLGLLLMVVGLPTVFLILLIIIYLGKGLIAFVNRFIPEEI
                     ILKNNDSESGIPDRVKRIIAAAVSEATKGKGIVTTIEKR"
     gene            complement(1137..3287)
                     /gene="scpA"
                     /locus_tag="PLI37_06725"
     CDS             complement(1137..3287)
                     /gene="scpA"
                     /locus_tag="PLI37_06725"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOQ58737.1"
                     /translation="MKPDFKNIDIYNPGIEERDPKTWEKAKGIQKNKKTPEGIELKSV
                     YTKADLEGMEHLHYAAGLVPYLRGPYSSMYVMRPWTIRQYAGFSTAEESNAFYRRNLA
                     SGQKGLSVAFDLATHRGYDADHPRVVGDVGKAGVSICSLEDMKVLFDGIPLNKMSVSM
                     TMNGAVLPIMAFYINAGLEQGAKLEEMAGTIQNDILKEFMVRNTYIYPPEFSMKIIAD
                     IFEYASKYMPKFNSISISGYHMQEAGATADIELAYTLADGLEYLRTGVNAGMDIDSFA
                     PRLSFFWAIGMNHFMEIAKMRAARMLWAKIVKQFNPKNPKSMALRTHSQTSGWSLTEQ
                     DPFNNVGRTCIEAMAAVLGHTQSLHTNALDEAIALPTDFSARIARNTQIYIQEETNVC
                     KQVDPWAGSYYIETLTKELAEKAWALIQEIEELGGMAKAIETGIPKMRIEEAAARTQA
                     RIDSKTQTIVGMNKYRREKEDPIDILEVDNTAVRKQQIERLNQLRANRNETEVQKALD
                     AITLCVGTGEGNLLELAVEAARLRASLGEISYACEKVVGRYKAVIRTISGVYSSETKN
                     DADFQKACSLVDSFAKKEGRQPRIMIAKMGQDGHDRGAKVVATGYADCGFDVDMGPLF
                     QTPEEAAKQAVENDVHIMGVSSLAAGHKTLIPQVIEEMKKLGREDILVIAGGVIPPQD
                     YDFLYKAGVAGIFGPGTSVAKAACEIMKILLDEE"
     gene            complement(3292..5154)
                     /locus_tag="PLI37_06730"
     CDS             complement(3292..5154)
                     /locus_tag="PLI37_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOQ58738.1"
                     /translation="MADSKEKLFSEFPPVDTQAWMDTIAADLKGADFEKRLVWKTNEG
                     FKVQPFYREEDLSQIKIKEALPGEFPFVRGNKTDNRWLVNQEFVVDKPETANKKALSA
                     LNNGVDSLTFVFSGKTFGNPEMGKLLEGIDPESVEINFRNCFQNNSLILGLFIDYLKA
                     KKVDLNKVKGSLMGDPFRQCLVFGKAYNAQWEEEVANLIAQSAELPLFQVLSANPYLF
                     NKAGSFIAQELGFGLAYGNAYMEALTDRGIEADKIAGKIRFQFGVSSDYFMEIAKFRA
                     ARLLWAKIVEAYLDGKACKSAGKMKIHALTSEWNQSVYDMYVNLLRSQTEAMSAGIAG
                     VDVLTVQPFDKAFKTPDEFSERIARNQQLLLKEEAGFSDVVDPSAGSYYIENLTMALA
                     EQAWNYFLTVQEKGGFTAALRDAYIQDEVNASNQKRLGFIAQRREILLGTNQYPNYDE
                     TAAGRIETELVPACDCANATLRPLNFSRGASAFESLRLATEKSGKRPKVFLLTIGNLN
                     MRLARAQFSGNFFACAGYEIIDNLGFETIEEGFKAARAAKADIVVLCSSDEEYAQLAP
                     EAYAALDKSKEILVIAGNPACSEELKALGITNFIHVRSNVLESLQTFNKQLNIH"
     gene            complement(5315..>5590)
                     /locus_tag="PLI37_06735"
     CDS             complement(5315..>5590)
                     /locus_tag="PLI37_06735"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HOQ58739.1"
                     /translation="LLMVQQGLVEEARSLYPYKGLNALNTVGYQELFDHFEDKCSLEE
                     AISKIQFNTHKYARKQLTWFRKDRDYHWFHPDREQDILNLIQNRLEK"
BASE COUNT         1351 a         1291 c         1355 g         1593 t
ORIGIN      
        1 tggtgataac atcgggatgt ccggtgcccc aggaaagagg ctccatgccg acatcgatgt
       61 agtcgcaacc tccgcggcaa acttccagaa tggaagccac attaaaaccc ggtccggcat
      121 ggccgtggta agatacggga atctcaggat gagctttttt gatgttttcg acaactttac
      181 ccaaagatac cggacgtcct ataccggcca tgtctttgat gcaaatttcg tccgcaccca
      241 ttttgatgag ttccagagcc ttgttggtgt aatattcgac ggtatggaca ggggaatgtg
      301 ttatgcagag cgagcattgt gaaatcatac cggcttcttt ggcataggta atggaaggag
      361 ctatgttgcg gagatcgttc aatccgcaaa aagttctggc gatatcggtg ccctgggctt
      421 ttttgacttt ataaaacaat ttacggacat cggcaggtac cggactcatg cgtatgccgt
      481 tcaatcccct gtcgagcata tgagtctgta tgccggcttc gcgaaagggt ttggtccatt
      541 cgcgaactgc cttattggga ttttctccaa acaacaggtt gacttgttca aatcctccgc
      601 cgttggtttc aacacgtgca aagcagccca tctttacgat gtggggtgcg atttttacca
      661 attgatcaac tctggggaca tattttccgg acgattgcca catatccctg aacatcaagc
      721 tgaatttgac ctctcttctc ttcataaatt acctcttttc tatggttgta actatacctt
      781 tgcctttggt ggcctccgat acggcggcgg caataattct tttaactctg tcgggaattc
      841 cgctttcgga atcgttgttt tttaatataa tttcttcggg aataaatctg ttgacaaaag
      901 caataagacc tttgcccaaa taaataatga taagaaggat taaaaagaca gtaggcagtc
      961 cgactaccat caacaacaaa ccaagactaa gattttccat aacgcttact tactaaaaac
     1021 gactccaacc cagtttagaa aaaaacgtca cgaagttact caaaaaaaat aagataacaa
     1081 ggctgtaaca ggctaaataa tcaaaagatt tttttgaaat tcaaaaagtt cggagtctat
     1141 tcttcgtcca ataaaatctt catgatttca caagcggctt tagcgacaga agtgcccggg
     1201 ccaaaaatgc cggcaacccc tgctttgtac aggaaatcgt aatcttgtgg aggaatcacg
     1261 cctccggcga taaccagaat gtcttcgcgt cccagttttt tcatttcttc gattacctgt
     1321 ggaatcaggg ttttatgtcc ggcagccaga gaggaaacac ccattatgtg cacatcgttt
     1381 tcgaccgctt gtttggcggc ttcctccggg gtttgaaaca gggggcccat atcgacgtca
     1441 aaaccgcagt cggcgtagcc ggtggcaacc actttggctc cgcgatcgtg tccgtcctgg
     1501 cccattttag caatcataat acgcggttga cggccttctt ttttggcaaa actgtcaacc
     1561 agcgagcagg ctttctgaaa gtctgcatcg tttttggttt ctgaagaata cacgcctgat
     1621 atggttctaa taacagcttt ataacggcct actacttttt cgcaggcata ggagatttcg
     1681 cccagactgg ccctaagacg ggcagcttct acggccagtt ccagcaaatt gccttcgccg
     1741 gtgccgacac aaagggtgat agcatccagg gctttttgca cttcagtttc gttgcggttg
     1801 gccctcagtt ggttgagccg ttcaatttgt tgtttgcgca cggctgtatt gtcgacttcc
     1861 agaatatcga tgggatcttc tttttcgcgt cggtatttat tcattccgac gatggtctgt
     1921 gttttggaat cgatgcgggc ctgagtgcgg gcagcggctt cttcgatacg cattttggga
     1981 atacctgtct cgatggcttt ggccataccg cccagttctt ctatttcctg aatcagtgcc
     2041 caggcttttt cggccagttc tttggtcagg gtttctatgt aataagaacc cgcccaggga
     2101 tccacctgtt tgcagacatt ggtttcttcc tgaatgtaaa tttgggtatt acgggcaata
     2161 cgggccgaaa agtctgtcgg cagggcaata gcttcgtcca gagcattggt atgcagagac
     2221 tgggtatgtc ccagcactgc tgccatagct tcgatacaag tacgtcctac gttgttgaag
     2281 ggatcctgtt cggtaagcga ccagccggag gtctgggagt gggtacgcaa agccatcgac
     2341 ttgggattct tcggattaaa ctgtttaaca attttggccc aaagcatacg ggctgcccgc
     2401 attttggcga tttccataaa atggttcatg ccaatggccc aaaagaacga aagccgggga
     2461 gcgaagctgt cgatgtccat tccggcattt actccggttc tgaggtattc cagcccgtcg
     2521 gcaagggtgt aggccagttc aatatcggcg gtggcgcctg cctcctgcat atgatagccg
     2581 gaaatcgaaa tggaattgaa tttgggcatg tacttggagg cgtactcaaa gatgtcggca
     2641 attattttca tcgagaattc cggcggatag atgtaggtgt tgcgaaccat aaattctttg
     2701 aggatatcgt tttggatggt gccggccatc tcttcgagtt tggccccttg ttccagcccg
     2761 gcgttgatgt aaaaagccat gatgggcaga acggcaccgt tcatggtcat ggaaaccgac
     2821 attttattca aaggaattcc gtcgaaaagt actttcatat cttcgagcga acagatagaa
     2881 accccggctt taccgacatc gccgacaaca cggggatgat cggcatcgta accgcggtgt
     2941 gtggccaggt cgaaggcaac ggaaagcccc ttttgtcccg aagccaggtt acgacgataa
     3001 aaagcgttgg attcttcggc tgtggagaaa ccggcgtatt gacgtatggt ccagggacgc
     3061 attacgtaca tggagctgta gggtcctctc aaatagggga ccagtccggc agcgtaatgc
     3121 agatgctcca tcccttcgag atcggctttg gtatagaccg attttaattc aatgccttcg
     3181 ggtgtttttt tgtttttttg tatgcccttg gccttttccc aggttttggg atctctttct
     3241 tctatcccgg ggttatatat atcgatgttc ttaaaatcag gtttcatttg cttagtggat
     3301 gtttagttgt ttgttgaatg tctgcaggct ttccagcacg ttgctgcgta cgtggatgaa
     3361 attcgttatg cccagggctt tgagttcttc gctgcatgcg ggattaccgg cgataaccag
     3421 gatttctttg cttttgtcca gtgcggcgta agcttcggga gccagttgag cgtattcttc
     3481 gtcgctggaa cacagcacta cgatgtctgc cttggcggca cgtgcagctt tgaaaccttc
     3541 ctctatggtt tcgaatccca gattgtcgat gatttcgtaa cccgcacagg caaaaaagtt
     3601 gcccgagaac tgggctctgg ccagtcgcat attcaaattg ccgatggtca gtaaaaaaac
     3661 tttaggccgt ttgcccgatt tttcggtggc cagcctcaag ctttcaaaag cagatgctcc
     3721 gcgactgaaa ttcagcgggc gcaaggttgc attggcacaa tcgcaggccg gtacaagttc
     3781 tgtttctatc ctgccggcag cggtttcatc gtaattgggg tattggttgg tacctagaag
     3841 tatttcacga cgttgtgcga taaatccgag gcgtttttga ttcgaagcat ttacttcgtc
     3901 ctgaatgtaa gcatcacgca gcgcggcagt gaagccgcct ttttcctgaa cggtcaggaa
     3961 ataattccag gcctgttcgg ccagggccat agtgagattt tctatatagt aggaaccggc
     4021 cgaaggatcc acaacgtccg agaagccggc ttcttcctta agcaataatt gttgattgcg
     4081 ggcaatgcgt tccgaaaact cgtcgggagt tttgaaggcc ttgtcgaagg gttgaaccgt
     4141 gagaacatcc accccggcaa tgccggcaga catagcttcg gtctggctgc gcagcaagtt
     4201 cacatacata tcgtaaacgc tctggttcca ttccgaagtg agggcatgaa ttttcatttt
     4261 acccgccgat ttgcatgctt ttccatcgag gtaggcttcc acgattttgg cccagagcag
     4321 ccgggctgcc ctgaatttgg caatttccat gaaataatcg gagcttacac cgaactggaa
     4381 gcgaatttta ccggcaattt tgtctgcctc gatgccccgg tcggtcaggg cttccatata
     4441 ggcgttgccg taagccagtc caaagcccag ttcctgagca atgaacgacc cggctttgtt
     4501 gaaaagataa ggattggcag ataatacctg aaacaaaggc aattctgcac tttgagctat
     4561 cagattggca acctcctctt cccattgagc gttgtaggct ttgccgaaaa caaggcattg
     4621 cctgaaggga tcgcccatca acgagccttt caccttattc aggtcaactt ttttagcttt
     4681 taaatagtct atgaacaggc ccaggatcaa ggaattgttt tggaaacaat tgcgaaaatt
     4741 gatttcgaca ctttcgggat caataccttc gaggagcttt cccatctcgg gattgccaaa
     4801 cgtttttccg gaaaacacaa aagtgaggga atccactccg ttgttcagcg ccgacaaagc
     4861 ctttttgttg gctgtttccg gtttatctac tacaaattcc tgatttacca gccagcggtt
     4921 atcggttttg ttgcctcgca caaaagggaa ttctccggga agagcctctt tgatcttgat
     4981 ttggctaaga tcttcttcgc gatagaaggg ttgtaccttg aagccttcgt tggttttcca
     5041 aacgagtctt ttctcgaaat cagccccttt taaatcggcg gcaattgtat ccatccaggc
     5101 ttgggtgtcg accggtggaa attccgagaa aagtttttct ttgctatctg ccatgataat
     5161 aaaatattga tttttagagt attacgagcg agtctatttg cgtggaataa atattcaata
     5221 agacctgcaa agataggtct ttttattctg accgtgaaaa gaataagcct tgtttttaaa
     5281 ccgtataaac cgggcttttg gaaccgggaa cggatcattt ttcaaggcgg ttttggatca
     5341 ggtttaaaat atcttgttcc cgatccggat gaaaccaatg ataatcccgg tcttttctga
     5401 accaggtaag ttgcttacgg gcgtatttat gggtattaaa ctgaatctta ctgatagcct
     5461 cttccaggct gcatttgtcc tcgaaatgat caaacaattc ctgataaccg acagtattca
     5521 atgcgttaag gcccttgtag ggatacaggc ttcttgcttc ttcgaccaaa ccttgttgta
     5581 ccataagcag
//
LOCUS       DAOSTD010000102         5508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_13509_length_5508_cov_15.352650, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000102 DAOSTD010000000
VERSION     DAOSTD010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13509_length_5508_cov_15.352650"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLI37_06740"
     CDS             <1..350
                     /locus_tag="PLI37_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58740.1"
                     /translation="VEAGQVQYVLGGSYKVADFHPWGILPNSKVKNDMRLNADFSFKN
                     SSALIRKIEENYSQATSGNKTVSLDLQADYVISRNLNLKFYYSLESSIPLVSSYPVTT
                     QDFGLSFRFSLTR"
     gene            complement(347..1198)
                     /locus_tag="PLI37_06745"
     CDS             complement(347..1198)
                     /locus_tag="PLI37_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58741.1"
                     /translation="MSFRVFAYHAFVGFCLIIMQSCVSNKKDLPDTYIQKARAAIELR
                     QYQTAKNYIDSIRIVFPKDYDKIREGLQVMREIEFAEQKRTREYCDSLLNVRQSQFPE
                     KQKNFSFQRNKAYESVGYYVNNHQLHKNNYNRTFLQTKVDEKGRLIVTSYYSGPRKIN
                     HTRLRIYSSDGLFVETQDVPRDGALNYAFKDGDINYEIVRFNEKKLNGLINFILLNNG
                     GTLKVELIGDVNRTYNMRKEDKDALKAACELAAVLSDITRLLEEIRLAQAKMDYIRSK
                     QLSRAED"
     gene            1281..2714
                     /locus_tag="PLI37_06750"
     CDS             1281..2714
                     /locus_tag="PLI37_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ58742.1"
                     /translation="MNLSEHIKDPLFILLSEAAEEMDVRCCLVGGYVRDIFLMVSSQD
                     IDIVTVGKGIELAELVAAKLGRSTRVNVFKNFGTAQLRYGDFELEFVGARRESYQRES
                     RKPIVEDGSLEDDLRRRDFTINAMAVSLNKADYGELIDPFDGRNDLRKKILRTPCDPD
                     LTFSDDPLRMMRALRFATTLQFNIDSATFEAIRRNKERISIVSAERIIDEIQKMMMAE
                     RPSKGFQLMDRSGLLDIILPELAALKGVETKDGKGHKDNFLHTLAVLDKVAAQSDNIY
                     LRWAALLHDIAKPLCKRWDDKLGWTFHNHNFLGEKKIPGIFRRLKLPMNEHMKYVQKL
                     VGLHMRPIALVEEEVSDSAVRRLLFDAGDDIEDLMILCEADITSKNPLKVQRFLENFQ
                     LVRRKMIDLEERDRIRNMQPPVSGEEIMDTFNLPPGKLVGEIKNSIKDAILDGLIPNE
                     YGAARDFMLQKGRELGLEPHEQEGSSS"
     gene            complement(2687..4252)
                     /locus_tag="PLI37_06755"
     CDS             complement(2687..4252)
                     /locus_tag="PLI37_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOQ58743.1"
                     /translation="MLAVFDPLSVLSREDLEQIQAKGIKTEQIQEQLKRFVQGFQYLK
                     LAASASAGNGILIIDHEHENSLLETWERYLKARHKVLKFVPASGAASRMFKDLYSYLE
                     SETEEPSGKAEKIFISHCGCFAFSDELSESCIKNLGASLESLQEQGRFKDIVRQLLMP
                     RGLNYGALPKGLLSFHKYPEGPRKAMTEHLVEAALYAPNYNGKAYVHFTVSPEHRELF
                     EKECRLVKPDIEQEYGVQIEVSFSIQKPSTDTIAADKNNLPFRDQGKLLFRPGGHGAL
                     LENLNDLDADIVFIKNIDNVVPDRLKDSTVHYKKLLAGLLVSLQEKIANYLKLLDTGK
                     YTRAQLVEIIQFLQQQLYTRHKDIKTMEDAELVLYLKKKLNRPIRVCGMVRNEGEPGG
                     GPFLVYEEDGSTSLQILESSQIDMKDPEKAAMYHEGTHFNPVDLVCGIKDYKGKKFDL
                     RRYVDKNTGFISIKSKSGQEHKALELPGLWNGAMSDWITVFVEVPISTFNPVKTVNDL
                     LREQHQDEDPSCS"
     gene            complement(4256..4777)
                     /locus_tag="PLI37_06760"
                     /pseudo
     CDS             complement(4256..4777)
                     /locus_tag="PLI37_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            4864..>5508
                     /locus_tag="PLI37_06765"
     CDS             4864..>5508
                     /locus_tag="PLI37_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ58744.1"
                     /translation="MIITQTEGRNIARMYRQLLQLTRGYILPEHIRKIRSIWEKAIED
                     NLLERPVPEVQPLERCVQNALVVAREIGMKRSSVISALLYDSRLIRSLELKQLERDFG
                     PSVGNIMRGLIKVNELYDRNIVFDNENFRNLLLSFAQDVRVILILLADRLNTMRMLKR
                     YPGEMQEKIAREVFSLYVPLAHRIGLYSIKTELEDLYLKFTQRAIYDEIARKLDE"
BASE COUNT         1351 a         1253 c         1350 g         1554 t
ORIGIN      
        1 tggtcgaggc aggacaagtg cagtacgtgt tgggaggcag ttataaagtg gccgatttcc
       61 atccctgggg aattttgcca aactccaagg taaaaaacga tatgagactc aatgccgact
      121 tctcttttaa aaattcctcg gctctgattc gaaaaatcga agaaaattac tcgcaggcaa
      181 cctcgggcaa caaaacagtt tccctggatt tgcaggccga ttatgtgatc agccgaaatt
      241 tgaatctgaa gttttattat tccctggaat ccagtattcc gctggtgtcg tcttatccgg
      301 tgacgactca ggatttcggt ttgagtttcc gctttagtct gacccgttag tcttcggccc
      361 ggctaagctg tttgctgcgg atataatcca ttttggcctg ggccaatctt atttcttcga
      421 gcagcctggt aatatccgaa agcacggcag ccaactcaca ggccgccttg agggcgtctt
      481 tatcttcctt gcgcatgttg taggttctat tgacatcccc tataagttcc accttgaggg
      541 tgcctccgtt gtttaacaaa ataaaattga tcaggccgtt gagtttcttt tcgttgaagc
      601 gtacaatttc gtagttgata tctccgtctt tgaaggcata attcaaagcg ccgtcccggg
      661 gtacgtcctg agtttccaca aaaagaccat cggaggaata aatgcgcagc ctggtatgat
      721 tgatttttct tggacctgaa taataactgg ttacaatcag acggcctttt tcatccactt
      781 tggtctgcag aaaagtccgg ttgtaattgt ttttgtgtaa ctggtggtta ttaacgtaat
      841 agcctacgct ttcgtaggcc ttgttgcgct gaaaactgaa atttttttgc ttttcgggga
      901 actgactctg tcgtacattt aataagctgt cgcaatattc ccgggtgcgc ttttgttcgg
      961 caaattctat ttcacgcatt acttgcaggc cttcgcgaat cttgtcgtaa tctttgggga
     1021 aaactatgcg tatgctgtct atgtagtttt ttgccgtttg gtattgcctc agttcaatgg
     1081 cagcccgggc cttttgaata taagtatcgg gcaggtcttt cttgttggaa acgcaagact
     1141 gcatgataat caggcaaaac ccgacgaacg catggtaggc aaaaaccctg aacgacataa
     1201 gttttgattt tagtccacac aaaagtagag taaacaatga aaaaaaatgt acttttgtcg
     1261 gctaatagta gttgtagttt gtgaatttaa gtgaacacat caaagacccc ctgttcattc
     1321 tcttatctga ggcagccgaa gagatggatg tgaggtgctg tttggtagga ggatatgtta
     1381 gagatatctt tctcatggtc agctctcagg acatcgatat agttacagta ggaaagggca
     1441 tagaattggc ggaacttgtg gccgccaaac tggggagatc taccagggtc aatgttttca
     1501 agaattttgg gacagcgcaa ctgcgttacg gtgattttga gttggaattt gtgggagcca
     1561 gacgggaatc ctatcaaaga gaatcccgta aacccattgt ggaggacggc agcctcgaag
     1621 acgacctccg gcgccgcgat tttaccatca atgctatggc cgtcagcctg aacaaagccg
     1681 attacgggga gttgatcgat ccttttgacg gtcggaacga tcttcgaaag aaaatcctgc
     1741 gtacgccttg cgatccggac ctgacattca gcgatgatcc gcttcgcatg atgcgtgccc
     1801 ttcgttttgc cacgaccctg cagttcaaca tagattccgc gacctttgag gcaattcgtc
     1861 gcaataagga gcgtatttcc atagtttcgg ccgaacgcat aattgatgag atacaaaaga
     1921 tgatgatggc agaacggcct tcgaaaggat ttcagctgat ggatcgctcc ggactgttgg
     1981 atattatcct gcccgaattg gcagccctca aaggggtaga aactaaggac ggcaaaggac
     2041 ataaagataa ttttttacat accctggccg ttttggacaa agtggcagct caaagcgaca
     2101 acatatacct gcgctgggct gccttgctcc acgatattgc caaacctctc tgtaaacgct
     2161 gggacgacaa attgggctgg acttttcaca accacaattt cctgggcgaa aaaaagattc
     2221 cggggatttt tcgccggctc aagctgccca tgaacgaaca catgaagtat gtgcaaaaac
     2281 tggtaggtct ccatatgcgt cccattgctt tggtcgaaga agaagtgagc gactcggctg
     2341 ttcgtcgtct gcttttcgat gccggcgacg atatcgaaga cctgatgata ctgtgtgagg
     2401 ccgacatcac gtcaaaaaat ccgctcaaag tgcaacgttt ccttgagaat tttcagttgg
     2461 ttcgccggaa aatgatcgat ctggaagagc gcgaccgtat tcgtaatatg caacctcctg
     2521 tgagcggaga ggaaataatg gatacgttca acttacctcc cggcaaattg gtgggagaaa
     2581 taaaaaacag catcaaagac gccatcctcg atggtctgat tccgaacgag tatggggcgg
     2641 ccagagattt tatgcttcaa aaaggccgtg aattggggct ggaacctcac gagcaggagg
     2701 gatcttcgtc ctgatgttgt tccctgagca agtcgttgac ggttttaacc ggattaaagg
     2761 tagatatggg tacttcgaca aaaaccgtaa tccagtcgga catggctccg ttccataagc
     2821 ccggtaattc cagagctttg tgttcctgtc cgcttttcga tttaatggaa ataaagccgg
     2881 tgtttttgtc cacataacgc ctgaggtcga attttttccc cttatagtct ttgatgccgc
     2941 agaccagatc taccggattg aaatgcgtgc cctcgtgata catggctgcc ttttcgggat
     3001 ccttcatatc gatctgtgaa ctttccaaaa tctgcaatga agtgctgccg tcttcctcgt
     3061 acactaagaa ggggcctccg ccgggttctc cttcgttgcg gaccatgccg caaacccgga
     3121 tggggcggtt gagtttcttt ttgagataga gcaccagttc ggcatcttcc atcgttttga
     3181 tgtctttgtg gcgagtgtat aattgttgct gcaggaactg gatgatttcg acgagctgag
     3241 cacgggtata tttaccggta tcaagtaact tcaaatagtt tgcaattttt tcctgcaggc
     3301 ttaccagcag gccggccagc agttttttgt aatgaacggt gctgtcttta agacgatcgg
     3361 gcaccacatt gtcaatgttt ttgatgaata caatgtccgc atccaggtcg ttcagatttt
     3421 ccagcaaagc cccgtgtccg ccggggcgga aaagcagttt cccctgatct ctgaaaggca
     3481 ggttgttctt atcggccgct atggtatcgg tagagggttt ttgtatggaa aaacttactt
     3541 caatctgcac tccgtactcc tgctcaatgt ccggttttac cagccggcat tctttttcga
     3601 acaactctct gtgctcgggt gaaacggtaa aatgcacgta ggcctttcca ttgtagttag
     3661 gagcatacag agcagcttcc accaaatgct cggtcatggc tttgcggggg ccttccgggt
     3721 atttgtgaaa actgagcagt cccttgggta aagctccgta attgagcccc cggggcatta
     3781 agagttgcct gacaatgtct ttaaaacggc cttgttcctg cagtgattca agcgatgcac
     3841 ccaggttctt tatgcatgat tcgctgagtt cgtcggaaaa agcgaagcat ccacaatggc
     3901 tgatgaaaat tttttcggcc ttacccgagg gttcttccgt ttccgattcc agatagctgt
     3961 agaggtcttt gaacattcgg ctggcagctc ccgaagccgg gacaaacttc agcactttat
     4021 gacgggcctt aaggtagcgt tcccaggttt caagcaaact gttctcgtgc tcgtgatcga
     4081 tgatgagtat gccgttaccg gcagaagccg aggcagctag ttttaggtac tggaagcctt
     4141 gcacaaacct cttgagctgt tcctgaattt gttccgtttt tatgcctttt gcctgtatct
     4201 gctcgaggtc ttcccggctc aggacgctga gcggatcaaa gacggcaagt atttgatccg
     4261 ctacttcttc ggggctgaag tgggaggtat ctatcaccaa atcgtaatga tcgagtttgc
     4321 gataatcgat gcgatagagc tttttgaatc gtctggcttc gacttcctgc ctttccaaca
     4381 agctgttttt gctgtcttca atatcggcgg tcaaaggctc gttgctgcgg gctttgtcgg
     4441 ccaataccct tctggcggct atttcaggct gtactttgag gtagacctta aaagcgcttt
     4501 ttacaaaatg ccaggccagc cgggaatcca aaaccatggg cttggtgatg cttttgtcgc
     4561 tgtcgatgtt tttgagaaaa gcatcaatgt agtcgtctat ctcgttggtc ttttccgata
     4621 ggtaattcaa ttccaaggtg tccattccct tttcggcagc taattttcgt tggagagaac
     4681 cggttgagaa atagtcgtaa ccggcctttt ggcatataat cttggctacg ctgcttttgc
     4741 cgctgcccaa gtctcctgta atggcgatat gtctcataat tttaggttgc aaataatcag
     4801 gccaaatata gcctattttg gtagaaaaaa cgtactttta catgactttt ttagtttgta
     4861 ctcatgatca tcactcagac cgaaggccgg aacattgcac gaatgtaccg tcaattgttg
     4921 caattgacac ggggatatat acttcccgaa catattcgga aaatacgctc catctgggaa
     4981 aaagccatag aggataatct gctggaacgt cccgtacccg aagtgcaacc tttggaacgc
     5041 tgtgttcaaa atgccctggt ggtggcacgc gaaatcggga tgaaacgttc gtcggtcatc
     5101 agcgccctgt tatacgactc caggctgata cgaagtctgg agcttaaaca actcgaacgg
     5161 gattttggcc cctcggtggg caatattatg cgagggctga tcaaggtgaa cgaactttac
     5221 gaccgcaaca ttgtttttga taacgaaaac tttcgcaatc ttttactctc gtttgcccag
     5281 gatgtccggg taatactgat ccttctggcc gatcgtctga ataccatgcg gatgctgaag
     5341 cgttatcccg gggaaatgca ggaaaaaatt gcccgggaag tgttttcgct ctacgtcccc
     5401 ctggctcacc gcattggtct ctattccatc aaaacagaac tggaggattt atacctgaaa
     5461 tttacccagc gggccatata cgacgaaatt gcccgcaaac tggacgaa
//
LOCUS       DAOSTD010000103         5371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_13929_length_5371_cov_12.904251, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000103 DAOSTD010000000
VERSION     DAOSTD010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13929_length_5371_cov_12.904251"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..979)
                     /locus_tag="PLI37_06770"
     CDS             complement(5..979)
                     /locus_tag="PLI37_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOQ58745.1"
                     /translation="MKRIFLLCCLSFSLLAFAAQEESKPAYDLVVAQDGSGDFTSIQE
                     AIHSLRAVMTERKIVFIKKGVYREKIVVPTYCTNITLLGEDRDNTIIRWGDHANMPIP
                     GTETNMGTFRTYTLLVQGHGFRAQNLTIENDAPQLGQAVALHVEGDKASFINCRILGN
                     QDTIFAGSETSRQYYRDCYIEGTTDFIFGSATAYFLNCRIHCKRNSYITAPSTPEYRK
                     YGFVFKECKVTADEGIRVYLGRPWRPHGNTVFVQCELGACIRPEGWDNWRNPENEKTA
                     RFYECLNTGPGAWQEGRVSWAKNLKKSSLKEYTLEAVLAGKENWDPEK"
     gene            complement(991..3180)
                     /locus_tag="PLI37_06775"
     CDS             complement(991..3180)
                     /locus_tag="PLI37_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOQ58746.1"
                     /translation="MSVLRFNLVQEAFSHKAVPADIPAKKVSDYYGIHVFHRETMSKY
                     LPGNIYNQLLKDIDSGKALNITMANHLAKAMKIWAMELGVTHYTHWFHPLTDGTAEKH
                     DALIQLEESGRVIEAFNGSLLIQQEPDASSFPSGGIRNTFEARGYSAWDPSSPAFVVD
                     DTLCIPTIFISYTGEALDYKTPLIKAISAVGKAAAETCRFFYPEVKKVNSNLGWEQEF
                     FLVDEALWSARPDLMMCGRTLMGHEAAKNQQLDDHYFGTIPKRVVEFMKELEYEAYKL
                     GIPLKTRHNEVAPNQFELAPIYEETNLAVDHNLLLMDIMNKVAPKHHFKVLLHEKPFD
                     GVNGSGKHCNWSLSTDAGLNVFSPGKTAEGKLMFTVFLVNTLMAVHKHNALLKASVAS
                     AGNARRLGLNEAPPAIVSVFLGSQLSGILDKMAESTAEEAIQIDPRGVLRLDVGQIPE
                     LMLDNTDRNRTSPFAFTGNRFEFRALGSSANCASAMIVLNTAVAGQLMDFNAALNAKM
                     AAGESKEKAVFSILRQYIRESKPIRFEGNGYSDEWKAEAAKRGLDCENSVPLQYDAYL
                     RPSSIALFEQTEVLNSKELYARNEVKWETYTKKVQIEARVLGDLALNHINPVAIRYQS
                     SLLDNLYKLKSLFSAEEYEELAQAKLQKVRIIEQYTDEIQQLVESMVDARKVANKIED
                     DREKAIAYHDTVEPFLDEIRQYIDKLELMVDNELWPLPKYRELLFIK"
     gene            3266..5350
                     /locus_tag="PLI37_06780"
     CDS             3266..5350
                     /locus_tag="PLI37_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ58747.1"
                     /translation="MNKVPFRHQILRFRRFIHKSYAVFNSLNKSVSIGKLSAAVCDLA
                     LKKSKTAGLLALLFTGIVSFSLQASDPEDPDWTVDLDSAGITATPPLSFPAHQRAVYS
                     VGHSQIAHFPAQHFNELLDALPGLDLRQRGQQGVQADLSMRGGSFDQVLVLLNGINIT
                     DPQTGHYNLDLPVDLAAIERIDVLQGSSLSLSGSPAFSGAVNIVTGYSGKNRLSVDLG
                     AGQYGWDRRSLQIQQKLKQWYLNASWSQVRSSGYIKNTDFDKQNLFVQLQAPSLPSGN
                     WNIQLGLQQKEYGANKFYSLLYPDQFEATQTLLSSLTWNKLLDRYKASASAYFRLHSD
                     RFELFRQTTAAPAWYRSHNHHLSDVSGLDLNLSRYGPAGKSMIGFNIRNEHILSTVLG
                     QPLEKSRRSAFLPDSVVYTNSAGRLGLTLFAEQQFNGPNFNSSLGLSVNKYAGYDARI
                     CAEAAFAYLFSDRSRLFVNAQRSLRYPSFTDLYYKSPVQVSNPNLKPETAYSAETGFS
                     YNEQRLQAGISLFGRLGSNIIDWVKQAGEEVWHSMNHSRVNAAGAEALISWKPQKHLR
                     LLESSYTFTILNKESGELMSKYALDYLKHKFNTRLEHSIYKDFAASWHFSAQQRMGTY
                     NDPEGNLQHYRPFCLLDGRLFWSRKELTLYLEAGNLFNTSYYDYGGILQPGRWLRAGI
                     RIQLDRDLLSNH"
BASE COUNT         1330 a         1407 c         1278 g         1356 t
ORIGIN      
        1 aaaattactt ctcgggatcc caattttctt ttccggccag aacagcttcc agggtgtatt
       61 cttttaaaga gctctttttc aagtttttag cccagcttac acggccttcc tgccaggctc
      121 cggggccggt gttgaggcat tcataaaaac gagccgtctt ttcgttttcg ggatttctcc
      181 aattgtccca gccttcgggg cgtatgcaag cacccagttc acattgcaca aaaaccgtgt
      241 tgccgtgggg cctccagggc cttcccaaat aaacccgtat gccctcgtcg gcagtaacct
      301 tgcattcttt gaaaacaaaa ccgtatttgc ggtattccgg tgtggaagga gccgtaatat
      361 aggaattgcg cttacaatgt atccggcaat tcaaaaaata ggcggtggcc gaaccgaaaa
      421 taaaatcggt cgtgccttcg atgtaacaat cgcggtaata ctgccggctg gtttcgctgc
      481 cggcaaagat ggtatcctgg ttgcccaaaa tccgacaatt gataaaactg gccttatcgc
      541 cttccacatg caaggctaca gcctgtccca attgaggagc gtcgttttcg atggtcaggt
      601 tttgagccct gaaaccatgc ccctgcacca acagagtata ggttctgaaa gtacccatgt
      661 tggtttcggt acccggtatg ggcatattgg catgatcgcc ccagcgaata atggtgttgt
      721 ctctgtcttc gcccaacaga gtaatgttgg tacaataagt gggcaccaca attttttcgc
      781 ggtagactcc ttttttgata aaaacgattt tacgttcggt cataacagca cgcagggaat
      841 gaatggcttc ctgaatactg gtaaaatcgc cgctgccgtc ttgtgccacc accaggtcat
      901 aagccggttt gctctcttcc tgagcggcaa aggcaagcag ggaaaaactc agacagcata
      961 aaagaaaaat tcgtttcata gctaagtgtt ttatttgata aacaataatt cacggtattt
     1021 gggcaggggc cacagctcat tatccaccat taattccaat ttgtcgatgt actggcggat
     1081 ttcgtccaaa aacggctcga ccgtatcgtg ataagcgatg gccttttcgc ggtcgtcttc
     1141 gattttgttg gccactttgc gggcatcgac catcgattcc accaattgtt gtatttcgtc
     1201 ggtatactgc tctatgattc ttaccttttg tagtttggcc tgagccagct cctcgtattc
     1261 ttcggcactg aacaggcttt taagtttata caagttgtcg agaagcgaag actggtaacg
     1321 gatggctacc ggattgatgt ggttcagggc cagatcaccc agcacacggg cttcgatctg
     1381 tacctttttg gtataggttt cccattttac ttcgttgcgg gcgtataatt ccttgctgtt
     1441 caacacctct gtctgctcga acaaagcaat ggacgaagga cgcaaataag cgtcgtattg
     1501 caggggcacg ctgttttcgc aatccagtcc tcttttggcg gcttccgcct tccattcgtc
     1561 gctgtagccg ttcccttcga agcgtatggg ttttgattcc cggatgtatt gacgcaaaat
     1621 ggaaaaaacg gctttttctt tggattctcc ggccgccatt tttgcgttca aagcggcgtt
     1681 gaaatccatt aattggccgg ccacggcggt attgagcaca atcatggccg aagcgcaatt
     1741 ggccgatgaa cccagcgctc tgaattcaaa acgattgcct gtaaaagcaa agggcgaagt
     1801 ccggttacgg tcggtattgt cgagcatcaa ttcgggaatc tgccccacat cgagacgcag
     1861 cacaccgcgc ggatctatct gaatggcctc ctctgccgta gattccgcca ttttgtcgag
     1921 tatgccgctg agctgactgc ccaaaaacac cgatacgatg gccggagggg cctcgttcag
     1981 ccctaggcgg cgggcattgc cggccgaagc caccgaagcc ttgagcaggg cgttgtgttt
     2041 gtgtacggcc atgagggtgt tcaccaaaaa gaccgtaaac atgagttttc cttcggctgt
     2101 tttgcccggc gaaaagacgt taaggccggc atcggtgctc aaagaccaat tgcaatgctt
     2161 acccgatccg ttcactccgt caaagggctt ttcgtgcaac aacactttga aatgatgttt
     2221 gggagccact ttattcatta tatccatcaa aagcaggttg tgatccacgg ccaggttggt
     2281 ctcctcgtaa atgggcgcca gctcaaactg attgggagcc acctcgttgt gtctggtttt
     2341 aagaggaata cccaatttat aagcctcgta ctcgagttct ttcataaact ccaccactct
     2401 tttgggaatg gtgccgaaat aatggtcgtc gagctgctga tttttggcag cctcgtgccc
     2461 catcagggtt cgtccgcaca tcatcaaatc gggacgcgca ctccaaagag cttcgtccac
     2521 caaaaagaat tcctgttccc aacccaggtt ggagttcact tttttgactt ctggataaaa
     2581 gaaacggcaa gtttcggcgg ccgctttacc gacagcggaa atggccttga tcagaggcgt
     2641 tttatagtcg agcgcttcgc cggtgtagga aataaaaata gtgggtatgc aaagggtgtc
     2701 gtccactaca aaggccggcg aagagggatc ccaggcgctg tatcccctcg cttcgaaagt
     2761 attgcgtatg cctccgctcg gaaaactcga ggcatcgggt tcctgttgaa taagcaggct
     2821 gccgttgaag gcttcgatca cccggccgct ttcttccaac tgtatcaaag catcgtgttt
     2881 ctcggccgtt ccgtcggtaa gcggatgaaa ccaatgggta taatgcgtca cacccaattc
     2941 catagcccag attttcatgg ccttggccag atgattggcc atggttatat tcaaggcttt
     3001 gcctgaatcg atgtctttga gcaattgatt gtagatgttg ccgggcaggt atttactcat
     3061 ggtttcgcga tgaaacacat ggatgccgta ataatcggaa accttttttg ccggaatatc
     3121 ggcaggaacg gccttatgcg aaaaagcctc ctgaacgagg ttgaaacgta acactgacat
     3181 aagaaagggc ttaactaatc agcaacaaag gtaagacaaa aaatttttta tctacctttg
     3241 tccccgtttt ttcgagcata caaaaatgaa caaggttccc ttcaggcatc aaatcttgcg
     3301 cttcagacgc tttatccaca aatcctatgc ggtattcaat tctttgaaca aatctgtttc
     3361 catcggaaaa ctgagcgcag cggtttgtga tcttgccctg aaaaaaagca agaccgccgg
     3421 cctgttggcc ttgctgttca caggtatcgt ttcttttagc ctgcaagcct ccgatccgga
     3481 agaccccgac tggacggtcg atctggatag tgccggaatc acggcaactc cccctttgag
     3541 ctttcccgcc catcaacgag ccgtttattc cgtaggccac tcgcagatag ctcattttcc
     3601 cgcacaacat ttcaacgaat tgctcgacgc ccttcccggc ctcgacctgc gacagcgcgg
     3661 tcaacagggt gtacaggccg atttgtccat gcgcggaggc agttttgacc aggtattggt
     3721 gctgctcaac ggcatcaata tcaccgatcc tcaaaccggc cattacaacc tggatcttcc
     3781 cgtcgatctg gccgccatag aacgcattga tgtgttgcag ggcagctcgc tgagcctctc
     3841 gggcagtccg gcttttagcg gagccgtaaa catcgtcacc ggctattcgg gcaaaaacag
     3901 gctgagtgtc gacttagggg cgggacaata cggatgggac cgccgcagcc tgcaaataca
     3961 gcagaaactg aaacagtggt atctaaacgc ttcctggtcg caagtgcgat catcgggcta
     4021 tatcaaaaat accgattttg acaaacagaa tctgtttgtt caactgcaag ccccctccct
     4081 gccttcgggc aactggaaca ttcaactggg cctccagcaa aaagaatacg gagccaacaa
     4141 attctattcc ctgctctacc ccgatcaatt cgaagccaca caaacactgc tgtcttcgct
     4201 cacatggaac aagctcctgg acaggtacaa agcaagcgct tcggcttatt tcaggttgca
     4261 cagcgaccgt tttgaactgt ttcgtcaaac aacagcggcc cccgcctggt acagatcaca
     4321 caatcatcat ttaagcgatg taagcggtct ggatttgaac ctgagccgtt acggccctgc
     4381 gggaaaatcg atgatcggtt tcaatatacg caacgaacac attctgagca ccgttttggg
     4441 gcagcctttg gaaaaaagcc gcagatcggc ctttctgccc gattccgttg tttataccaa
     4501 cagcgccgga cgtcttggcc tgaccctttt tgccgaacaa caattcaacg gccccaattt
     4561 caactcttcc ttagggttat cggtcaacaa gtatgccgga tacgacgccc ggatctgtgc
     4621 agaagccgcc tttgcctatt tattttcgga tcgcagccgt ctttttgtca atgcacaacg
     4681 cagcctgagg tatccgagtt ttaccgatct ctattacaaa agtccggtac aagtcagcaa
     4741 tcccaacctc aaacccgaaa ccgcctacag tgccgaaacg ggtttcagtt acaacgaaca
     4801 acgattacag gccggaataa gcctttttgg ccgcctgggc agcaacatca tcgactgggt
     4861 gaaacaagcc ggcgaagaag tttggcacag catgaaccac agccgtgtaa atgctgccgg
     4921 cgccgaagct ctgatcagct ggaaaccgca aaaacacctt cgtttattag aaagttccta
     4981 cacctttacc atactgaaca aagagtccgg agagctgatg tcgaaatacg ccctcgatta
     5041 cctgaaacat aaattcaaca cccgactgga acactctatc tacaaagact ttgccgcaag
     5101 ctggcacttc agcgcccaac aacgtatggg cacctacaac gatccggaag gaaatctgca
     5161 gcactaccgc cccttctgcc tgctggacgg ccgcttattt tggagcagaa aagaactgac
     5221 cctctacctg gaagccggca acttgttcaa cacaagctat tacgattacg gaggcatcct
     5281 tcagcccgga cgatggttga gggcgggcat acggattcaa ctggaccggg atttgttatc
     5341 aaatcattaa ggagattttc gggcaaagcc t
//
LOCUS       DAOSTD010000104         5292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_14213_length_5292_cov_12.832538, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000104 DAOSTD010000000
VERSION     DAOSTD010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14213_length_5292_cov_12.832538"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..2415)
                     /locus_tag="PLI37_06785"
     CDS             complement(7..2415)
                     /locus_tag="PLI37_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOQ58748.1"
                     /translation="MKKLFILALTLSLAAGMQAKTQKSHHGYPIDPVPFTSVKVTDAF
                     WGQRLKASREVTIPLAFSKCEETGRYENFVRAANPSSEYKVEGYSFDDTDVYKTIEGA
                     SYSMQTYPDKALDKYIDSVIVIMAAAQEPDGYLFTARTQNPEHPHEWAGSKRWEKVED
                     LSHEFYNLGHLIEGAIAHYQATGKRSLLDIAIKYADCVCREIGDGEGQTVAVPGHQIA
                     EMALAKLYLLTGEQKYLDQAKFFLDKRGYTSRRDQYSQAHLPVIEQDEAVGHAVRAAY
                     MYSGMADVAALTGDSSYTKAIDRIWENIVSKKLYITGGIGATGSGEAFGRNYELPNMS
                     AYCETCAAIGHVYMNYRLFLLHGESKYYDVLERTLYNGLISGVSLDGGRFFYPNPLES
                     MGQHQRQAWFGCACCPSNVCRFIPSIPGYVYATKDRAVFVNLFMSNNTDLRLNNKKIN
                     LQQSTNYPWDGDISITVNPVGRQDFILKIRIPGWVRNQVVPSNLYSYNDGKRLNFSIA
                     LNGKELPAQELTDGYFCIDRKWSKGDKVEVHFEMQPRTVKANPNVEADKGKIAVERGP
                     IVYCAEWPDNDFDILSTFMNQKPQFELIEKPDLLSGIIQLRTDAQVLRYDDRGRLQTQ
                     DVKLNLIPYYAWAHRGSGNMAVWLAQDLSASRPTMPPTLASESKISVSHRAGSISSIN
                     DRLVPNSPEDRSVPYYHWWPKENTVEWIAYEFKEEAEISSTMVFWFDDGPWGGCRVPK
                     AWKIYYKDSQGNWQPVNNIDEYGRQKGLPNQVRFEPVKTTAVKLEVALPERHASGVFE
                     WEIN"
     gene            complement(2694..>3582)
                     /locus_tag="PLI37_06790"
     CDS             complement(2694..>3582)
                     /locus_tag="PLI37_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ58749.1"
                     /translation="VRIGLASEGFMQFDNFRLTCFGADEPATAEAPADYTFAIANPSF
                     EDGLGGTLDTTSVLGSGNHNVPAGWNLYLNQSGWCNAVSITDAPSDGASAFETWAATI
                     NRIDLYQNIVIPATGVYRLTADVRTNTPTPYTQHVYAKTKFGTFESETLDSTSVITGD
                     GWNGMENWQTLSAEFEAPVGMTVRAGLASQGFMQFDNFKLLYFGANDTTGVVGLGAVK
                     ALPTLMIYPNPASDFIVVSGIENNSVVKVFNVTGQQLYVRRADQNQLSIDLNGLNQGL
                     YILQVESEGQVINSKFIKK"
     assembly_gap    3583..3682
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3683..>5292)
                     /locus_tag="PLI37_06795"
     CDS             complement(<3683..>5292)
                     /locus_tag="PLI37_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58750.1"
                     /translation="KLSKTHIAGLQIYGVALTQEDLQSEELTNISARIAALNTAYNEN
                     PEVVGPEFQQEYDALTLPDISALTEETVLTLPTQGTIEPAIKITWVSSQPEYVSNTGV
                     VTQPKYFDKKIVLTATLNQGTTFKSKTFEGKVLVKGGFTTDLVANFDFSKGSGTTVTD
                     QGEFAFEGTLHEEAYIATIGEGDNAIKVLYLANGDGYFDMGKEIGKAVSGLTDYTVSV
                     FFRKDNNQGELSQFTGYGQWLYGFANTNNLGGQAYGAMYYEPLRDRHVCTPDNYGAEP
                     NNHVGVGEGNTFVGSWHNITFTQTGDTGVLYKDGWAAATAKISNPQTALWRSGLSGTI
                     HNYIGKPCYSGDPYLQNTMIASLVIYKKGMTEEEVNTLFDIENTIVKLDAAYALNDGS
                     ASVRAIDLQNLVDEARDYAALAYPGLDALNEAITAAEAAIAGNNVTEEDIDDLKTAIR
                     MYRFTQPASGAAPADFTFAIENPSFEGTFGGKLDPNSIVEGDGSYKYPTGWTLYMDKT
                     GWCNAVDITAGPSDGTKAFETWAATIRK"
BASE COUNT         1195 a         1203 c         1354 g         1440 t
ORIGIN      
        1 aacgatttaa ttgatttccc actcgaaaac gccggaagcg tggcgctcgg gcagggcaac
       61 ttccagtttt accgctgtgg ttttaaccgg ttcaaatcgt acctggttag gcaaaccttt
      121 ttggcgtccg tactcgtcga tgttatttac aggctgccaa ttgccctggc tgtctttgta
      181 atagattttc caggctttgg gaaccctgca acctccccag ggaccatcgt cgaaccaaaa
      241 gaccatggtg gaggatatct cggcttcttc cttaaattcg taggcgatcc attccaccgt
      301 attttctttg ggccaccagt gataataggg tacggaacgg tcttcgggac tgttgggtac
      361 caaacggtcg ttgatggatg aaatggaacc tgctctgtgc gaaacgctaa ttttgctttc
      421 ggaagccagg gtgggcggca tggtggggcg cgaagcgctg agatcctgtg ccagccagac
      481 ggccatatta ccactgccgc gatgtgccca ggcgtaataa gggatcagat tcagtttgac
      541 atcctgagtc tggagtcggc ctctgtcgtc gtaacgaagc acttgagcat ctgtgcgtaa
      601 ttgaataatg cctgaaagaa gatcaggctt ttcaatcaat tcaaactgag gcttttggtt
      661 cataaaagta cttagtatat cgaaatcgtt gtcgggccat tcggcacaat aaacgatggg
      721 accgcgttca acggcaattt ttcctttatc ggcttccaca ttgggattgg ctttgaccgt
      781 acggggctgc atttcgaaat gcacctccac tttatcgcct ttactccatt ttctgtcgat
      841 gcagaaatag ccgtctgtca attcttgtgc cggcaattct ttaccgttga gcgcgatgga
      901 aaagttcagg cgctttccgt cgttgtagga atacaaattg ctgggaacaa cctgatttct
      961 tacccatccg ggtatacgga ttttcaatat aaagtcttgt cttccgacag gattcacggt
     1021 tatgctgatg tctccatccc aagggtagtt ggtgctttgt tgaagattga tttttttgtt
     1081 gttcaatcta aggtcagtat tgttcgacat gaaaaggttg acaaaaactg cccggtcttt
     1141 ggtggcgtaa acatagcccg gtattgatgg aatgaaacgg caaacgttgg aggggcagca
     1201 ggcacagcca aaccaggctt gacgttgatg ttggcccatt gattccagag gattgggata
     1261 gaaaaaccgt ccaccgtcca gtgagactcc ggaaatcagt ccgttataca aagtgcgctc
     1321 caaaacatcg taatattttg attcgccatg cagcaggaaa agccggtagt tcatatacac
     1381 atggccaatg gcggcacaag tttcgcaata agccgacata ttgggtaact cgtaattcct
     1441 tccaaaagct tcaccgctgc cggtggcgcc gattcctccc gtgatataga gtttcttgct
     1501 tacaatattt tcccagattc gatcgatggc tttggtgtag gaggaatctc cggtcagggc
     1561 agccacatcg gccattcctg aatacatata agctgcacga acggcatgac ccacagcttc
     1621 gtcttgttca atcaccggca ggtgagcctg gctatattgg tctctgcgcg aagtgtaacc
     1681 ccttttgtcc aggaagaact tggcctgatc gaggtatttc tgttcaccgg tgagcaggta
     1741 taatttggcc agagccattt cggcgatctg atggccggga actgctacag tttggccttc
     1801 tccgtcgccg atttctctgc aaacacaatc tgcgtatttg atggcaatat ccagaaggct
     1861 gcgtttaccg gtggcctgat agtgggcaat agctccttct atcaggtgtc ctaggttgta
     1921 aaattcgtgg ctgagatctt cgactttttc ccagcgtttc gatccggccc attcgtgggg
     1981 atgttcggga ttctgggtgc gggccgtgaa taaatagccg tccggttcct gggcggcagc
     2041 cataataacg atgacgctgt caatgtattt atccagcgct ttgtcgggat aggtctgcat
     2101 ggaatagctg gctccttcta tggttttgta cacatcggtg tcgtcgaaag aatagccttc
     2161 cactttgtat tctgaacttg ggttggcagc ccgcacaaaa ttttcgtaac gtccggtctc
     2221 ttcgcatttg ctaaaggcca gaggaatggt tacttcacgg ctggctttga gcctttgtcc
     2281 ccagaaggcg tccgttactt tgaccgatgt aaaaggcacc ggatcgatgg ggtaaccgtg
     2341 atgactcttt tgtgttttgg cttgcatacc tgcagccaga ctaagtgtta gtgcaagaat
     2401 aaatagtttt ttcataactg tcgtttatag gataatgaat taaaaggttg agtctgaaga
     2461 tagtaattta tcggcaaatt acctttcacg ttgggtttaa ccgcgacact aagtgacaca
     2521 tacaaagaaa atcaattcac ccgtaaagaa gtgcaatgaa aatccagaag gctgtaacta
     2581 ttgtccgatt tttaggctaa ttgttggtga tggactacaa tgcagccgga atatagacaa
     2641 aaagaaaggc cgggcaaatt tgctcggcct ttccgatata gagcttgttt acattacttc
     2701 tttataaact tgctgttgat aacttgtcct tcggattcaa cctgcaggat gtacaagcct
     2761 tggttaagtc cgttcagatc gatactcagt tggttttggt cggctctgcg aacgtagagt
     2821 tgctgaccgg ttacgttgaa taccttgacc acggagttgt tttcaatacc gctgacaacg
     2881 atgaaatcgc ttgcgggatt ggggtagatc atcagggtcg gcagggcttt gactgcaccc
     2941 agacctacaa cacccgtagt gtcgttagct ccgaagtaaa ggagtttgaa attgtcgaac
     3001 tgcatgaagc cttgcgaggc cagtcctgcg cgaaccgtca tgccgacggg agcttcaaat
     3061 tcagcgctca gcgtctgcca gttctccatg ccgttccagc catcaccagt aatgacactg
     3121 gttgaatcga gggtttcgga ttcaaaggtg ccgaatttgg ttttggcgta tacatgctgg
     3181 gtataaggag tcggagtatt ggtgcgtacg tcggccgtca ggcgataaac accggttgcg
     3241 gggataacaa tgttttggta aagatcaatc ctgttgatgg tggctgccca ggtttcaaat
     3301 gcactggctc cgtcggaggg tgcatcggta atactgacgg cattgcacca gcctgattga
     3361 ttcaggtaca gattccaacc tgcggggacg ttgtgatttc cactaccgag aacactggta
     3421 gtatcgagtg tgcctcccaa accatcttcg aagctcggat tggcgatagc aaaagtatag
     3481 tctgcaggag cctctgcggt ggcgggctca tcggcaccaa agcaagtaag cctgaagttg
     3541 tcgaattgca taaagccttc cgaagccagt ccgatgcgga cgnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnaacttccg tatggttgct gcccaggttt cgaaagcctt
     3721 ggttccgtcg gagggaccgg cagtaatgtc aacggcattg caccagccgg tcttatccat
     3781 gtacaaggtc caacctgtgg gatatttata gctgccgtcg ccttcgacta tgctgttggg
     3841 atcaagtttt ccgccaaagg ttccttcgaa gctcgggttc tcaatggcaa aagtgaagtc
     3901 tgcaggagct gcacccgaag ccggttgagt gaaacgatac attctgatgg cggtcttgag
     3961 gtcatctatg tcttcttcgg tgacgttgtt gcctgctatg gctgcttcgg cagcggtaat
     4021 ggcctcattg agagcatcaa gtccggggta agccaaagcg gcataatccc tggcttcgtc
     4081 taccaaattc tgaagatcga tggcccgtac gctggccgac ccgtcgttca gggcataggc
     4141 ggcatccagt ttcacgatgg tgttttcgat gtcaaatagg gtattgactt cttcctcggt
     4201 catacctttt ttgtagataa ccaaagaggc aatcatggta ttctgcaggt aggggtcgcc
     4261 cgaatagcag ggtttaccga tgtaattgtg gatggttccg ctcagtccgc ttctccacaa
     4321 ggcggtttga ggattactga ttttggctgt ggcagcagcc caaccgtctt tgtaaagcac
     4381 tccggtgtcg ccggtttgtg taaaggtaat gttgtgccag cttcccacaa aggtattgcc
     4441 ttcgccgacg cccacatggt tattgggttc tgcaccgtag ttatcgggtg tacaaacatg
     4501 gcggtcgcgc agcggttcgt aatacatggc accgtaagcc tggccgccca ggttgttggt
     4561 gttagcgaat ccgtacaacc attgtccgta gcctgtaaac tgcgacaatt ctccctggtt
     4621 attgtcctta cggaagaaaa cactgacggt gtagtcggtg agacccgaaa cggctttacc
     4681 gatttctttg cccatgtcga agtagccgtc gccgttggcc aaatagagca ctttgatggc
     4741 gttgtcgcct tcgccgatgg tagcaatata agcttcttcg tgcagggttc cttcaaaggc
     4801 aaattcaccc tgatccgtga cggtggtacc actgcccttg gagaagtcga aattggcaac
     4861 aagatcggtt gtaaatccgc ccttaaccag tactttgcct tcgaaagttt tgctcttgaa
     4921 ggtggtacct tggttcaatg tggcagtcag tacaatcttt ttatcaaagt atttgggttg
     4981 tgtaactact cccgtgttgg aaacatactc cggctgactg gatacccaag tgattttgat
     5041 ggcgggctct atggttccct gggtgggaag ggtaagaacc gtttcttcgg ttaatgcact
     5101 gatgtcaggc aaggtcagcg catcgtattc ttgctggaat tccggaccta ccacttcggg
     5161 attctcgttg taagcggtgt tcagggcagc gattcttgca ctgatattgg tcagttcttc
     5221 gctctgcagg tcttcctggg taagagcaac tccgtatatc tgcaagccgg caatatgagt
     5281 tttggacaat tt
//
LOCUS       DAOSTD010000105         5236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_14423_length_5236_cov_13.821270, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000105 DAOSTD010000000
VERSION     DAOSTD010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14423_length_5236_cov_13.821270"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1763
                     /gene="pruA"
                     /locus_tag="PLI37_06800"
     CDS             129..1763
                     /gene="pruA"
                     /locus_tag="PLI37_06800"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOQ58751.1"
                     /translation="MSTGFYTIPEIRNEPVKTYAPGTPERAALKKTLAELRSKEADIP
                     MVIGGREIRTGKKVRIFPPHEIKHTLGYYYQGDGSHVQMAIDAALAARDKWAAMSWQH
                     RSAIFLKAADLLAGPYRQKINAATMLGQSKNAFQAEIDAACELADFFRFGVRCVTDIY
                     KMQPESSPGMWNYNEFRPLEGFVFALTPFNFTSIAGNLPAAPAMLGNVAVWKPSKTAI
                     YSAAVIMELFREAGLPDGVINLVFADGPTTSNVVLNSPYFAGIHFTGSTGVFQSIWKT
                     IGENIYKYKSYPRIVGETGGKDFIFADPTADPQALTIALLRGAFEYQGQKCSAASRAY
                     IPAGLWKDVRERLGKELATVKMGPPEDFSNFINAVIDEASFDKLKGFIDRARTDADAE
                     VIFGGNCDKSVGYFIEPTVILAKKPDYITMIEELFGPILTIYVYKDEELDATLDLLDR
                     SSIYGLTGAIFSQDRYNIEKMTKKLVNAAGNFYINDKPTGAVVGQQPFGGARGSGTND
                     KAGSVFNFLRWTSIRTIKETFVPPTDYMYPNFLPGD"
     gene            1859..2401
                     /locus_tag="PLI37_06805"
     CDS             1859..2401
                     /locus_tag="PLI37_06805"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOQ58752.1"
                     /translation="MNKAIRKIGLVAHDGMKQDLIEWAVWNSERLIGHKFYCTGTTGK
                     LVQEALIRKHPDIDWDITILKSGPLGGDQQMGSRIVEGEVDYCFFFTDSMTLQPHDTD
                     IKALTRLAGVENIVFCCNRSTADHVISSPLFLDPGYKPIKKDYSSYTNRLKDRDLISK
                     AVESSRSQKRRKKRSKPGNE"
     gene            2423..4447
                     /locus_tag="PLI37_06810"
     CDS             2423..4447
                     /locus_tag="PLI37_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58753.1"
                     /translation="MKKYFVVISVVLLMISCKKTADTSLPESTVQQALTACIAQSELP
                     DDLIEKGLRQCAGLWTPADGSPEEFVEFCKIHLCADTQQKSLLFERMADHFETLLGHQ
                     NRVRVELMRPLHETGYQNLPVDQLFGAYDNAAHFEEDMYGSKIAFVVALNFPYFSLDE
                     KTALAEEWTSEEWGYARLGDFFIERIPASVKQEMTLAATRAEDYISNYNIHMGSLVDR
                     GEKLFPGDMVLISHWGLRDELKSNYADKERGLQKQEIIYRVMQHIIEQSIPLQVIDNP
                     NYDWDPINNKLYLEGKEIAVEAESNTRYEHFLNLFKTYKAQDNYSRQYPSYIARYFEG
                     ELEIAEKEVEALFVELLSSKQAALTGQLIAQRLGRKLRPYDIWYDGFKSRSSIDPAVL
                     DKIVLDKYPSLQAFEQDLPVILVKMGFSKQKAKEICAQIEVNPSVGSGHAWPAAMKND
                     KIHLRARFRENGVDYKAYNIATHEFGHNVEEYISLYDVPNYFLSSVPNNAFTEALAFV
                     FQERDLDLLGLKQQDPLAASLNTLDLFWSSYEIMGVALVDMKVWHWMYENPSTDAAQL
                     KENVIRIAREVWNDYYAPVFGIEDQNILAVYSHMIEIPLYLLAYPLGHLIHFQLNEHF
                     EGKDMGAEIQRIFALGRLSPQYWMQKAVGSKLSAEPLLKATSLALEQVMQ"
     gene            complement(4505..4978)
                     /locus_tag="PLI37_06815"
     CDS             complement(4505..4978)
                     /locus_tag="PLI37_06815"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ58754.1"
                     /translation="MGAELQFELCRWDNSRQTGAFLSLLNHYMADPMGNYPPLDGAEQ
                     QKLLADLRTHPTVEVLLMSLNGQYIGMTTFFVNYSTFRLKPYIYIHDVVVHKTRRNRG
                     YGQSMIKELVRLAQERDYCKLTLEVRNDNPAAQKVYSELGFEAGRPEMLFWTKWL"
BASE COUNT         1392 a         1352 c         1232 g         1260 t
ORIGIN      
        1 agtcgggtgt ttttaaccct cgtggacaat tcgcttattt atcggtacca ttttagggat
       61 tgggggtctg aaaatttgat aattttacgc tcggaataca aaacctatta cttattacat
      121 tcaacgctat gtcaacagga ttttatacga taccggaaat caggaatgag ccggtaaaaa
      181 cttatgcgcc gggtacgccc gaaagagctg ctttgaaaaa aactctggcc gaactcaggt
      241 cgaaagaagc ggatattccc atggtgatcg gaggccggga aatccgcacc ggtaaaaaag
      301 tacggatctt tccgccccac gaaatcaaac ataccctggg ctattattac cagggcgacg
      361 gcagtcatgt gcaaatggct atcgacgcag ccctggcggc ccgcgacaaa tgggcggcta
      421 tgtcctggca acatcgttct gccattttcc tgaaggctgc cgacttgctg gccggcccct
      481 accgtcaaaa aataaacgcc gccaccatgc tggggcaatc caaaaacgct tttcaggccg
      541 aaatcgatgc cgcctgcgaa ctggccgact ttttccgttt tggtgtgcgt tgcgtaacgg
      601 atatttacaa aatgcaaccc gaatcctcgc ccggtatgtg gaattacaac gaattccgtc
      661 cgctggaagg ctttgtgttt gccctgacgc ctttcaattt tacttccatc gccggcaacc
      721 tgcctgcagc cccggccatg ctgggtaatg tggcggtgtg gaaaccctcc aaaacagcca
      781 tttattcggc ggccgttatt atggaattat tccgtgaagc cggtttgccg gacggcgtta
      841 tcaacctggt gtttgccgac ggacctacga cttcgaacgt ggtattgaat tccccctatt
      901 ttgccggcat ccactttacc ggctctaccg gcgtattcca aagcatctgg aaaaccatag
      961 gcgaaaacat ctataaatac aaatcttatc cccgcatcgt gggcgaaacc ggaggtaaag
     1021 attttatctt tgccgatccc accgccgatc cccaggcctt gaccattgcc ttgctgcgcg
     1081 gcgcttttga ataccagggc caaaaatgtt cggctgcctc gagagcatac attccggccg
     1141 gtttgtggaa agatgtccgc gaacgcttag gtaaagagct ggccaccgtc aaaatgggcc
     1201 cgcccgaaga ctttagcaac tttatcaacg ccgtgattga cgaagcttct ttcgacaagc
     1261 tcaagggctt tatcgaccgc gcacggacgg acgccgatgc cgaagtgatt ttcggcggca
     1321 actgcgacaa atcggtgggc tattttatcg agcccacggt gattttggcc aaaaagcccg
     1381 attacatcac catgatcgag gaattgttcg ggccgatact taccatttac gtgtataaag
     1441 acgaagaact ggatgccacc ctcgatctgc tggatcgttc ctctatttac ggcctgaccg
     1501 gagccatctt ttcgcaggac aggtataaca ttgagaaaat gaccaaaaag ctggtgaacg
     1561 cagcgggcaa tttctacatc aacgacaaac ctaccggagc tgtggtaggc cagcaacctt
     1621 ttggcggagc cagaggctcg ggcaccaacg acaaggcggg ttcggtattt aatttcttgc
     1681 gctggacatc gatacgcacc atcaaagaaa cctttgtacc tcccacggat tacatgtatc
     1741 ccaatttctt gcccggggac taaaaccgac attgcttatt gcaaaaaaaa atcgttcctt
     1801 tgttctcttt gtcgaagcgg cttcgctgcc ttgacaagtt gtttttaacc ataaagctat
     1861 gaacaaagca atcagaaaaa tcggacttgt tgcccacgac ggtatgaaac aagacctgat
     1921 cgaatgggcc gtatggaact cggaacgcct gatcggacat aaattttatt gcaccggcac
     1981 caccggcaag ctggtacagg aagccctcat cagaaaacac cccgatatcg actgggatat
     2041 taccattctg aaatccggcc cgctgggcgg cgatcagcaa atgggctccc gcatcgtgga
     2101 gggcgaagtg gattattgtt tcttctttac cgactccatg actttgcagc ctcacgatac
     2161 cgatatcaaa gcactgaccc gcctggccgg tgtcgaaaac atcgtgtttt gctgcaatcg
     2221 ctctactgcc gaccacgtca tcagcagtcc cttgtttttg gatcccggct ataaacccat
     2281 taagaaggac tacagctctt ataccaaccg cctcaaagac cgggatctga taagcaaagc
     2341 ggtggaaagc agccgttccc aaaaaagacg taagaaacgc tctaaacccg gaaacgaata
     2401 aaaacagaaa aaaacgatcg ccatgaaaaa gtattttgtt gttatttcag ttgtcctgct
     2461 tatgatttcc tgtaaaaaaa cggccgatac aagccttccc gaaagcaccg ttcagcaggc
     2521 tctgaccgct tgcatcgcgc aatctgaact accggatgat ctcatcgaaa aaggtctgcg
     2581 tcaatgcgcc gggctctgga ctccggcaga cggcagcccc gaagagtttg tggagttttg
     2641 caaaattcac ctctgtgccg acactcaaca aaaaagcctt ttgttcgaac gtatggccga
     2701 ccatttcgaa accctgctgg gacaccaaaa cagggtcaga gtggaattaa tgaggcccct
     2761 gcacgaaacg ggctatcaaa atctgccggt cgatcagctt tttggagctt acgacaatgc
     2821 tgcccatttc gaagaggata tgtacggcag caaaattgct tttgtggtgg ctttgaactt
     2881 tccttatttc agcctcgacg aaaaaacagc cctggccgaa gaatggactt ccgaggaatg
     2941 gggctatgcc cggctgggcg atttctttat tgaacgcatt cccgcctccg tcaaacagga
     3001 aatgaccctg gccgccacca gagccgagga ttatatttcg aactacaaca tccacatggg
     3061 cagccttgtg gatcggggcg aaaagctttt ccccggtgat atggtgctta tctcccactg
     3121 gggcttgagg gacgagctta aatcgaatta cgccgacaag gaaagggggc tgcaaaaaca
     3181 ggaaatcatc taccgggtga tgcaacacat tatcgaacag agtattcccc ttcaggtaat
     3241 cgacaatccc aactatgatt gggaccctat caacaataag ttatatctgg aaggcaagga
     3301 aatcgccgtt gaagctgagt ccaatacccg ctacgagcat tttctgaatc tctttaaaac
     3361 ctacaaagca caagacaact acagtaggca atatccctct tacattgccc gctattttga
     3421 aggcgaactg gaaattgccg aaaaagaggt ggaagctctt tttgtagaac tgctctcgtc
     3481 gaaacaggcc gccctaaccg gccaattgat agcacaaagg ctgggccgca aactgaggcc
     3541 ctacgacatt tggtacgacg gcttcaagag cagaagcagc atcgacccgg ctgtgctcga
     3601 taagatagtg ctcgataagt atccctcttt gcaggccttc gagcaagacc tgcccgtcat
     3661 tctcgtaaaa atgggtttca gtaagcaaaa agccaaggaa atctgcgctc aaatcgaagt
     3721 caatccttcg gtgggctcgg gccatgcctg gccggcggcc atgaaaaacg acaaaatcca
     3781 tctccgggcc cgcttccgcg aaaacggcgt ggattacaag gcctacaaca ttgccaccca
     3841 cgaatttggc cacaacgtgg aagaatatat ttccttgtac gatgtgccca actacttcct
     3901 gagttcggtg cccaacaatg ctttcaccga ggctttggcc tttgtgtttc aggaaagaga
     3961 cctggacttg ctgggtttaa aacagcaaga tcccctggcc gccagcctaa ataccctcga
     4021 tttgttttgg agttcttacg agattatggg cgtggcgctg gtagatatga aggtatggca
     4081 ctggatgtac gaaaacccct cgaccgacgc agcccaactg aaggaaaacg tcatccgcat
     4141 agccagggaa gtctggaacg actattacgc cccggtgttt ggtatcgaag accaaaacat
     4201 actggccgtt tattcgcata tgatcgaaat tcccctttat ctgctggctt accccctggg
     4261 gcatttaatt cattttcaat tgaacgaaca cttcgaaggc aaagacatgg gagccgaaat
     4321 tcaacgcatc tttgccctgg gacgccttag cccccagtac tggatgcaaa aagcggtcgg
     4381 gtcgaaactc tcggcggagc ctttgctcaa ggctacttca ctggccttgg aacaagttat
     4441 gcaataacag attattttaa aaaaagccca acctctctgg acaggctcag gtttatcaat
     4501 ataattacag ccatttggtc caaaaaagca tctcgggacg gccggcttcg aagcccagtt
     4561 cgctgtacac tttttgagcg gccgggttgt cgttgcgtac ctcgagtgtg agtttgcaat
     4621 agtcgcgttc ttgcgccaaa cgtaccaatt ctttgatcat cgattgaccg tagccccggt
     4681 tgcgccgcgt tttatgtacc actacgtcgt gaatataaat gtaaggttta agcctgaaag
     4741 tggaataatt cacaaagaaa gtcgtcatgc caatgtattg cccgttcaga ctcatcagta
     4801 agacctcgac cgtgggatgt gttctcagat cggccagcaa cttttgctgt tctgcaccgt
     4861 cgaggggcgg ataattgccc atgggatcgg ccatataatg attcaacaaa gatagaaacg
     4921 ctcctgtctg cctgctgttg tcccagcggc ataattcaaa ttgcagttcc gctcccataa
     4981 tccacttgtt ataagcttgt taagataagt actcgtgtcc gcccggcttc ggacaggtgt
     5041 aagtcttcac gcgttaatag cccgcaaaga taggtatttt acagcttttt cactctgcag
     5101 aaaaatcgca tttaaagccc gcaaatgtag gtaatttacc ggatacgttt tgcattaaag
     5161 cacaaaactc tatctttgta gggtttaaac ctcatcccat gaaacgaaac tccctgttca
     5221 aagacaatta ctggct
//
LOCUS       DAOSTD010000106         5134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_14778_length_5134_cov_14.578067, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000106 DAOSTD010000000
VERSION     DAOSTD010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14778_length_5134_cov_14.578067"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="PLI37_06820"
     CDS             <1..1178
                     /locus_tag="PLI37_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOQ58755.1"
                     /translation="TSLDYSPYVGRIAVGRVYRGSFRPNQDVSLIKRDGTVIKSRIKE
                     LNEFVGFGRKPSAEVSSGDICAMVGIEQFDIGDTIADVNTPEALKPIAIDEPTMSMVF
                     TINNSPFFGKDGKYITSRHIHERLMRELDKNLAMRVEKDPERDIWTVFGRGVLHLSIL
                     IETMRREGYELQVGQPRVIIKEIKGEKCEPIEEMTINVTEEAASKMIDVVSRRKGELT
                     KMESKNGRVHLEFEIPSRGIIGLRSLLLTLSSGEAVVAHRFKEYQPYKGEIEKRENGS
                     MIAMETGVAVAYGMDKLQDRGRFFIHPGDEVYAGQVVGESNKEGDLVVNVNKTKKLTN
                     VRASGSDEKVSLPPPVIFSLEEALEYIKADEYVEVTPRFMRLRKIILDENERKRHNK"
     gene            1608..2600
                     /locus_tag="PLI37_06825"
     CDS             1608..2600
                     /locus_tag="PLI37_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOQ58756.1"
                     /translation="MYVSREDQMGTNITLKLGMPQHGRCDVDTLGSICRGELTFLRVP
                     TTIKRTQPILSNTKLQSSTIPFLKPVNAKDSILTERVSPLRFRQGSAQLFRTYMDNDE
                     TLEAIDEALNILQYDDRARLSRITIESFTSPEGDPELNRDLSQRRAKTLRDEIVNLHP
                     EISDNQFEIISGVEDWQGLRDLVAQSVMPYRDEILDIIDNTAENSVRKLKLKRLRGGV
                     PYNSLIDLYCPLLRDACYVHIWFEEKPDMEAMIINDAIENIEQNLLDDALELLSTVEY
                     DQRSWNALGTCYLIKGEVSRARHYFVQAVDAGNGDAKSNLLLIGTKETFDLDAN"
     gene            2647..3873
                     /locus_tag="PLI37_06830"
     CDS             2647..3873
                     /locus_tag="PLI37_06830"
                     /inference="COORDINATES: protein motif:HMM:NF028454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5119 domain-containing protein"
                     /protein_id="HOQ58757.1"
                     /translation="MVAAVKFYAKLRFTALIIITMIMGAVLSGCERKDLYLRVDQAEI
                     NIAIYDIQLDLLWQPSWRLELDYEWLAKYGPLGYPEKPELVKGIIYDVDTIEHKRTSY
                     FSSIFNSTGGRVSLKTRCTYNMMFYNFGTEWILFQQSEDYEYYNATTRTSSQTSWTRT
                     ASESEDSEMPEAHKSYIEVNQPDAIYGVLTDDLYVSDDPGEYEKEFNEDGRPVYIYKV
                     DCVLDPYVFIYIFQFVVKNNVDEEGQIITGAHGITVTGLSQGVEMFSRKTFDNTISIT
                     TEDIKEMQHHKNIKLQGDSAAFDGDIFVARMLTWGLPGINPMEKTLNNEPVEVIDNNY
                     VGVGLKLRNGYTYTITRDITEQMHKRPTGGIITVVIDAGKVPEEYLEHKPETGGGGFN
                     ASVEDWENEIEAELVI"
     gene            3922..5088
                     /locus_tag="PLI37_06835"
     CDS             3922..5088
                     /locus_tag="PLI37_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrillin family protein"
                     /protein_id="HOQ58758.1"
                     /translation="MKTKMKQIKEKTTKGNFLLSVLIAVVAIVFAACSSDVVLNDIAE
                     SQTDTPKAIGFTSFSEKITRADTDLEYYHNTFVVYGTKQSTVDNTIQYVFGGKATAAG
                     NQEGVTCTYNATAGVTGNWEYVNPRYWDKQAEYDFIAYAPANAPLRYYYGAADAQVGD
                     ANNEFVTNADYTLAGTNLQATATTAEKVKGFTGAGVDLDLMISNPKPQSGSNNADVAL
                     VFKHILSKLNVTFAKADVLDDATVTVTSVEITGLKEKGSYKQSDYVNDEFTKTSGWAA
                     KEVTGSTYKLAYSNGQELNASDGLPYLFIESLVMPQTIDDDQVTLKAKYTIQTGGYSE
                     NYTYQLDLHDVEALRKYFDGYNYTLKFTIQPNVIKFDANVTVWDSQPASNQTIN"
BASE COUNT         1548 a         1078 c         1205 g         1303 t
ORIGIN      
        1 ttacttcgtt ggattattct ccctatgtcg gacgcatagc cgtgggtcgc gtgtaccggg
       61 gaagctttcg tcccaatcag gacgtcagcc tgataaagcg agacgggacg gtgataaaaa
      121 gccgcatcaa ggagctgaat gaatttgtcg gttttgggcg taaaccgtct gccgaggtga
      181 gttcgggcga tatttgcgct atggttggca tagagcagtt tgatattggc gataccatag
      241 ccgatgtgaa cactcccgaa gctctgaagc ccattgccat agacgaaccc accatgagca
      301 tggtgtttac catcaataat tcaccctttt tcggaaaaga cggcaaatac atcacctcga
      361 ggcatataca cgaacgcttg atgcgggaat tggataaaaa cctggctatg agggtggaaa
      421 aagatcccga gcgggatatc tggacggtgt tcggcagggg cgttttgcat ctttcgatat
      481 tgatagaaac catgcggcgc gaagggtatg aacttcaggt aggacaaccc agggttatca
      541 tcaaggaaat aaagggagag aaatgcgaac ccatcgaaga aatgaccatc aatgtcaccg
      601 aagaagccgc cagtaaaatg atcgatgtag tcagccgccg taagggtgag ttaaccaaaa
      661 tggaaagcaa aaacgggcgc gtacatctgg aatttgagat tccctccaga gggattatag
      721 gtttacgcag cctcttgctc actttatcgt cgggcgaagc cgtcgttgct catcgtttca
      781 aagaatatca accctataag ggcgaaatcg aaaagagaga aaacggctct atgatcgcca
      841 tggaaacagg tgttgccgtg gcttacggta tggataagtt acaagaccgg ggtcgttttt
      901 ttattcatcc cggcgacgaa gtttatgccg gccaggtggt gggcgaaagc aacaaagaag
      961 gcgacttggt tgttaacgta aataagacga aaaaattaac aaatgtgcga gcttcgggtt
     1021 ccgacgaaaa agtcagcctg cctcctcccg taattttttc gctcgaagaa gccttggaat
     1081 acataaaggc cgatgaatac gtagaagtca ctcctcgttt tatgcgtttg cgcaaaatta
     1141 ttttggacga aaacgagcgg aaacgacaca ataaatagtt aaaagcacaa cgtttttcgt
     1201 aagtgtaaat gttaaaaatt agaagcccct tctttttttt aattttttac tacgtttgac
     1261 acaattttat tgtgtatttt cgtagctgtc tgaaactggg cttagattat tttattttaa
     1321 ggttgttggt ttttaattgt ttgaccggca gatagttgcc ggatactttt ctttgccgtt
     1381 ccagaccatg aattatgtaa aatatcggct gattgctatg ttatattagc aaacacttgt
     1441 tgtatgtccg gtaagttgtt ggtgtttaaa accggcgtgc cgtatttcag cacgggcatt
     1501 tttaattttg aaacagaagt taaagtgcca cagagtcgca gtttagcccc gggcggagtc
     1561 tataattctg tttttttaat agaacatggc acgatctttg ctttataatg tacgtatcgc
     1621 gcgaagatca aatgggaaca aatatcactc ttaaattagg tatgccacag cacggacgct
     1681 gtgatgtgga tacattggga tcaatttgca ggggcgaatt gacctttttg cgtgttccca
     1741 ccacgatcaa aagaacccag cctatcctca gcaacacgaa acttcagagt tcaactattc
     1801 cttttttgaa gccggtaaac gcaaaagatt ccatcctgac cgaaagagta tctcctttgc
     1861 gtttccgtca gggcagcgct cagttattca ggacttacat ggataacgat gaaacccttg
     1921 aggctataga cgaagctttg aatattttgc aatacgacga tagagcccgg ttatcaagga
     1981 ttaccattga gagctttact tctcccgaag gagatccgga attaaaccgc gatttatccc
     2041 aaagaagggc caagactttg cgcgacgaga ttgtcaattt acatccggaa atttccgaca
     2101 atcagtttga aattatttcc ggtgttgaag attggcaagg tttacgcgat ctggttgcgc
     2161 aaagtgtgat gccttatcgc gacgaaatcc tggatatcat cgacaatacc gccgaaaact
     2221 cggtaaggaa gttgaaattg aaacgtctta gaggaggtgt accctacaat tccctgatcg
     2281 atttgtattg tcccctgctc agagatgctt gttacgttca tatctggttc gaagaaaagc
     2341 ccgatatgga agcaatgatc attaatgatg ccatcgaaaa tatcgaacaa aatctgttag
     2401 acgatgcttt ggaattgtta tcaacagtag aatacgatca gcgttcctgg aatgctttgg
     2461 ggacctgtta tttaataaag ggagaagtgt caagagccag acattatttt gtacaggctg
     2521 tggatgcagg caacggcgat gccaaatcga atcttctgtt gattggaacc aaggaaacgt
     2581 ttgatctgga tgcgaattga tgtagtggat tagtggacat taaaaatata aaataggaac
     2641 aagagcatgg ttgcagcagt caaattttac gcaaaactcc ggtttacggc ccttatcatc
     2701 atcacgatga taatgggggc tgttttatcc ggttgtgagc gtaaagatct ttatctgcgt
     2761 gttgaccaag ctgaaataaa tattgcgata tacgacatac agttggattt gctctggcaa
     2821 ccgtcgtggc gtcttgagct cgattatgaa tggctggcca aatacggtcc tctgggctat
     2881 cccgaaaagc ctgaacttgt taaaggaatc atttacgatg tggatactat agagcacaag
     2941 agaacaagtt atttctccag catattcaat tcaacgggcg gccgtgtatc tttgaaaacc
     3001 cgctgcactt ataatatgat gttctacaac ttcggaacgg aatggatatt gttccaacag
     3061 agcgaggatt atgaatatta taatgctaca acccgaacca gttcacagac ttcgtggaca
     3121 agaaccgcat cggagagtga ggacagcgaa atgccggaag cccacaaaag ctacatagaa
     3181 gtgaaccaac ccgatgcaat ttacggagtt ctcaccgacg atctttatgt atcggacgat
     3241 cccggagaat atgaaaaaga atttaacgaa gatggcaggc cggtttatat atacaaagtc
     3301 gactgcgtac tcgaccccta cgttttcata tacattttcc agttcgtagt caaaaataat
     3361 gttgacgaag aaggacaaat cattacggga gcgcatggaa ttaccgtaac gggactttca
     3421 cagggtgttg agatgttcag tcgtaaaacc ttcgacaaca cgatttcgat aacgactgag
     3481 gacataaagg agatgcaaca ccataagaat attaaattac agggtgacag tgccgcattt
     3541 gacggcgata ttttcgtggc cagaatgctt acctggggtc tgccgggcat caatcctatg
     3601 gaaaagaccc tgaataacga acctgttgag gttatcgaca acaactatgt aggcgtaggc
     3661 ttgaaacttc ggaacggtta tacatacact ataacacgcg acataaccga acaaatgcac
     3721 aagcgcccca ccggaggtat aataaccgta gtaatcgatg ccgggaaagt gcccgaagag
     3781 tatctggaac ataagcccga aacgggcgga ggcgggttca acgccagcgt tgaggattgg
     3841 gaaaatgaaa ttgaagccga attagtgatc taaaaaacaa gatagaatca tattattaac
     3901 tcatattaaa atcaaatcaa aatgaaaaca aaaatgaaac aaatcaaaga aaagacgaca
     3961 aaaggaaact tccttttgag tgtattgatt gcagttgtcg caatcgtgtt tgcagcttgc
     4021 tcgagcgatg tcgttctcaa tgatattgcc gagtcacaga ccgatacgcc caaggctatc
     4081 gggttcacat cgttctccga aaagatcacg cgtgccgata ccgatctgga atattaccac
     4141 aacacattcg ttgtgtacgg taccaagcag tcaaccgttg acaataccat ccagtacgtg
     4201 ttcggcggaa aggctacagc agcaggcaat caggagggtg taacatgcac ctacaacgcg
     4261 accgccggtg ttacgggcaa ttgggagtat gtcaaccccc gttactggga caaacaggct
     4321 gagtatgatt tcatagctta tgctccggca aatgctccgc tccgctacta ttacggcgca
     4381 gcagatgctc aggttggtga tgcaaataac gagtttgtca ccaatgcgga ctatactttg
     4441 gccggcacga acctgcaggc cacagccact acagctgaaa aggtaaaggg ctttaccggc
     4501 gccggtgttg atcttgacct tatgatatcg aatccaaaac cgcaaagcgg aagcaacaat
     4561 gccgacgtgg ctctggtatt caagcacatc ctatcaaagc tgaatgttac ttttgccaag
     4621 gcggatgtac ttgacgatgc aactgtgacc gtcaccagcg ttgagataac gggtctcaaa
     4681 gagaaaggca gctataagca gagcgactat gttaacgacg aattcacaaa aacaagcggc
     4741 tgggctgcca aagaggtaac cggcagtacc tacaaactgg cctactccaa cggacaggag
     4801 ctcaatgctt cagacggctt accttacctt tttatcgaat cgctggtaat gcctcagaca
     4861 attgacgatg accaagtgac acttaaagca aagtacacca tacagaccgg cggttacagc
     4921 gagaactaca cctaccaact tgatctgcac gatgtagaag ctttgcggaa gtacttcgac
     4981 ggttacaatt acacgctcaa gtttaccatt cagcctaatg taatcaagtt tgacgcaaat
     5041 gtaaccgtat gggatagcca gcctgcttca aatcagacca taaattaatc acacaaattt
     5101 tatttacatt tacaatcata tttataatca catt
//
LOCUS       DAOSTD010000107         5071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_14998_length_5071_cov_14.124003, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000107 DAOSTD010000000
VERSION     DAOSTD010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14998_length_5071_cov_14.124003"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLI37_06840"
     CDS             complement(<1..639)
                     /locus_tag="PLI37_06840"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOQ58759.1"
                     /translation="MKEGYLRKPEWLKIRLGDSEQYRITKQIIERHRLHTICTSGRCP
                     NQGECWSRGTATFMILGEICTRSCKFCNTLTGKPLPPDINEPEKVAESIRLMKLRYAV
                     LTSVDRDDLPDLGCSHWVAVVNKVREYNPGIVVETLIPDFQGRRELIAEIARLAPEVV
                     SHNLETVARLTPLIRSAAKYRRSLDVLEQLGAEGITTKSGLMLGLGETEEELM"
     gene            complement(636..2834)
                     /locus_tag="PLI37_06845"
     CDS             complement(636..2834)
                     /locus_tag="PLI37_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOQ58760.1"
                     /translation="MRTFRVPLILLCLIFQLSTVKAAGIELDSIIAGAYRPEIPSGWQ
                     SMNEGSSYACLAQGGKLILRYDYKSGKLLDTLLNVEKTRNVRMEKIEGFEFNPQESMI
                     LVWKNKKPIWRRSFTAEYFVYDRLHNILDSLSAVAPVREASFSPDGRMVVFSRNNNLF
                     IKKLDFKTEVAVSTDGKTNEIINGTADWVYEEEFMTTRMYEWTADSKFLCFVRFDERA
                     VPEHFFSMYGASKPEYPEYKYHPGEKRFRYPRSGENNSKVSLHCYNVQYRTLQKIELP
                     VDEETYIPRLRRMENPDLVAVMTLNREQNLFRMFTANPKSLLSRLILSESSDTYYDPS
                     YLDDILFTDKDFTYVSERDGYRHLYLYSVNGAFIRQLTSGPNDLTNYYGRDLRKNYFY
                     YQAVDDLPYRRSLYRVDARGRTTKLSDKDGINQAVFDKHFNYYIWTWSDRNTPPVSVL
                     CQSDNAKVISTLKDNAALRQKLSALSLPQKEFFETPAADGQKLYGWMLKPAGFDPSLR
                     YPLVMLQYSGPDSQDVLDLFGIGWEYYLAARGIIVVSVDGRGTGGRGEQFRKQTWLKL
                     GQYEVQDQIAAARALGTLPYLDAARIGIWGWSYGAYISLLALTEGTQYFKAGIAVAPV
                     TDYRYYNTAYTERFMRTPQQNKENYDKTSVLQRASSLKGELLLIHGLADDNVHANQTM
                     ELIDALVQEGKQFQMQLYPNRNHSILGLSNRKHLYTRMSDFFIQTLNADK"
     gene            2929..3858
                     /locus_tag="PLI37_06850"
     CDS             2929..3858
                     /locus_tag="PLI37_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536715.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOQ58761.1"
                     /translation="MHKLYYSFPEFIGQYFKGKIQKISLDAGFSCPNRDGSLGRGGCS
                     YCNNLSFNPSYSSTKRSISEQLAEGKEFFAHKYPKMDYLAYFQSYTNTYDKLDRLIDL
                     YEEALRFPGVKGLVIATRPDCLPDDLLDYLEQLAKRCFLLVELGLESTLDRTLKEVGR
                     GHDYACSVNALNKIAQRRILCGAHIILGLPGESKEDMLSHAGRLNTLPLTTLKLHQLQ
                     LIRGTRLEEQVRNNPAYVHFFDAGSYARLLIDFIERLRPGLVLDRFVSQAPAEWLIGP
                     DWGLKNYEFTHQLEKMMRAGGHYQGRLYSGSNN"
     gene            complement(3866..4726)
                     /locus_tag="PLI37_06855"
     CDS             complement(3866..4726)
                     /locus_tag="PLI37_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ58762.1"
                     /translation="MSGSKEVIVVTGASAGIGKAIVQSLAGCGIRLIMACRNMEKSAA
                     VKAILEQDNPGAEIELLNLDLASFASIRQFASTLARKKYSINTLINNAGVLPGRFSLT
                     RDGFETTLGVNYLGLFLLNELLLPLMRPGSLIINTISCSYIIGRLNKDMFIPNPGARF
                     MSIFSYARSKRAILVYSLELAERLSGQGIRVLATDPGIVSTKLIAMKRWFDPLTDALF
                     RPLIKTPGQGADTAVFLTLEAQKPASEYAHLSGLCFKNRKAMRLPAKIRDKKIRKELW
                     DWTTAAIQGR"
     gene            4819..>5071
                     /locus_tag="PLI37_06860"
     CDS             4819..>5071
                     /locus_tag="PLI37_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58763.1"
                     /translation="MRNKLQFLAILLQGGLLLIFCANHVQARAEQADVSFLQSLINDS
                     QVLLAQAVEGQQEGNYLPGSKQDFQTALEPALALLNDSTA"
BASE COUNT         1272 a         1228 c         1232 g         1339 t
ORIGIN      
        1 catcaattcc tcttcggttt cacccaaacc caacatcagg ccggacttgg tggtgattcc
       61 ctctgcgccc aattgttcca gtacatccag gctgcggcgg tatttggcgg ctgaacgtat
      121 taggggagtc agacgggcga cggtttccag attatgcgac accacctcgg gagccaggcg
      181 ggctatttcg gcaatcagct ctctcctgcc ctgaaaatcg ggaatcagcg tttcaacgac
      241 gataccggga ttgtattctc tcaccttgtt cacgacagct acccagtggg aacaacccaa
      301 atcgggcaga tcgtctcgat ctaccgaggt gagtacggca taccttaatt tcatcaaacg
      361 aatggattcg gctacttttt cgggttcgtt gatatcgggg ggcaggggtt tgcctgtcag
      421 ggtattgcaa aacttacagg aacgagtaca tatttccccc aagatcataa aggtggctgt
      481 tccccgactc cagcattctc cctggttagg gcagcgaccg ctggtacaga tggtgtgcag
      541 gcgatgtcgt tcgataattt gtttggtgat acggtattgc tcgctgtcgc ccaggcgaat
      601 cttgagccat tcgggtttgc gcaaatagcc ttccttcatt tgtcagcgtt caaagtttgt
      661 ataaagaagt cggacattct ggtataaaga tgcttgcggt tggataaacc caaaatgctg
      721 tgatttcggt tggggtagag ttgcatctga aactgtttgc cttcctgtac cagagcatcg
      781 atcagctcca tggtctgatt ggcgtgcacg ttgtcgtcgg ccaggccatg gatcaacaac
      841 aactctcctt taagggaaga agcgcgttgc aataccgagg tcttgtcgta attctctttg
      901 ttttgctgag gggtacgcat aaatcgctcc gtatatgccg tgttgtaata acggtaatcc
      961 gtgacgggag caaccgctat gccggctttg aaatattgcg tgccttcggt cagggccagc
     1021 aagctgatgt aagcaccgta actccagccc caaatgccga tgcgggcggc gtccaagtag
     1081 ggcaatgtac ccaacgccct tgcagcggca atctgatcct gtacttcgta ctggccgagc
     1141 ttgagccagg tttgtttcct gaattgctct cccctgcccc ctgttccgcg gccatccaca
     1201 ctgaccacga ttattccccg ggctgccagg taatattccc agccgattcc aaagagatcc
     1261 agcacatcct gactgtcggg gccgctgtat tgtaacatca ccaggggata tcgaagggag
     1321 ggatcaaaac cggcgggttt cagcatccat ccgtagagtt tttgcccgtc ggcagcaggc
     1381 gtttcgaaaa attctttttg cggcaacgac aaagcgctca atttttgcct aagagcggca
     1441 ttgtctttaa gtgttgaaat taccttggcg ttatcagact ggcataaaac cgacaccgga
     1501 ggagtgttcc tatcggacca ggtccagatg tagtagttga agtgtttatc aaaaacggcc
     1561 tgattgatac catccttgtc actgagtttg gttgttctgc ctctggcatc caccctgtag
     1621 agcgaacgcc tgtaaggaag gtcgtccacc gcctgataat aaaaataatt cttacgcaga
     1681 tcacgtccat aatagttggt taagtcgttc gggcctgacg tcaattgacg gataaacgcg
     1741 ccgttgacac tgtacaaata caaatgtctg tatccgtctc tttcgctcac ataggtaaaa
     1801 tccttatccg tgaacaggat atcatccaaa tacgagggat cgtagtatgt atcgctgctt
     1861 tccgacaata tcaggcggct gagcaaggat ttgggattgg ccgtgaacat gcggaataag
     1921 ttttgctccc tgttgagcgt cataacagct acaaggtcgg gattttccat acgacgcaaa
     1981 cgaggtatat aggtttcttc gtccaccggc agctctattt tttgcaaggt gcgatattgc
     2041 acattgtaac aatgcagact cactttgctg ttgttttctc cgctacgggg ataccggaat
     2101 cgcttttctc ccggatggta tttatattcg ggatattcgg gctttgaggc tccgtacata
     2161 ctaaagaaat gctcgggcac tgccctttcg tcaaagcgga caaaacaaag gaatttactg
     2221 tcggccgtcc actcgtacat acgggtggtc atgaattcct cttcatacac ccaatcggcg
     2281 gtcccgttta tgatttcgtt ggttttcccg tcggtgctga ctgctacttc agtcttgaaa
     2341 tccagttttt ttatgaacag gttgttgttt ctggaaaaaa ccaccattct gccgtccggc
     2401 gaaaaagagg cctctctgac cggagccacg gccgaaagag aatccaaaat attgtgcaag
     2461 cgatcgtaca caaaatattc tgccgtaaat gaacgccgcc agatcggttt tttgtttttc
     2521 cataccagaa tcatactttc ctgggggttg aactcaaaac cttctatttt ttccatccgt
     2581 acgtttcggg ttttttccac attcaacaaa gtatcaagca gtttcccgct tttgtagtcg
     2641 taacgcagta ttaatttacc cccttgtgcc agacaggcgt aagagctgcc ttcgttcata
     2701 gattgccagc cggagggaat ttccggtcgg taggcccccg ctataataga atccaactca
     2761 atgccggctg cttttacagt agatagttga aatatcaggc atagcaatat tagtggtaca
     2821 cgaaaagttc tcataagaaa agggtctgaa acattgaaag atgggcaaag gtaaaaaatt
     2881 gttccgactt gcagaaggct ttaattaagt ttgtactttt gccgggttat gcacaaactt
     2941 tactacagtt ttcctgaatt tatcggccaa tattttaaag ggaagattca aaaaatctct
     3001 ttggatgcgg gtttcagttg tcccaatcga gacggtagcc tgggacgggg aggttgcagt
     3061 tattgcaata atctgagctt taatccttct tatagcagca ccaaacgcag tataagcgaa
     3121 caactcgccg agggaaaaga attctttgcc cataaatacc caaagatgga ttatttggct
     3181 tattttcaat cgtataccaa cacttacgat aagctggaca gactaataga cttatacgaa
     3241 gaagccttgc gtttccctgg ggtgaaggga ttggtgatag ccaccaggcc cgattgtctg
     3301 ccggatgact tgctcgacta ccttgagcaa ctggcaaagc gctgctttct gctggtggaa
     3361 ctcgggctgg aatccacctt agacagaacg ctgaaagaag tgggaagggg gcacgattac
     3421 gcctgctcgg taaatgcttt gaacaaaata gcccaacgcc ggattttatg cggagcgcat
     3481 attatactgg gcttgccggg agaaagcaaa gaggatatgc tctcgcatgc cggacgcctg
     3541 aatactttac ccttaaccac tcttaaactg catcagttgc agttgatacg aggaaccagg
     3601 ctggaagaac aagtcaggaa caacccggcc tatgtgcatt ttttcgatgc cggttcctac
     3661 gcgcgcctgc tcatcgattt tatcgagcgc cttcggcccg gtcttgtcct ggatcgtttt
     3721 gtttcgcagg ctcccgccga atggctgatc ggcccggact ggggattgaa gaattacgaa
     3781 tttacccacc aactggaaaa aatgatgcgt gccggagggc attaccaggg caggctctac
     3841 tccggttcga acaattgata aggaattagc gaccttgaat ggcagccgtt gtccaatccc
     3901 aaagctcttt acggatcttt ttatccctga tttttgcagg aagcctcatt gctttacggt
     3961 ttttaaaaca aaggccgctc agatgggcat attccgatgc gggtttttgt gcctccaggg
     4021 tgagaaaaac agccgtatcc gctccctgcc cgggtgtttt gatcaggggg cggaacaaag
     4081 catcggtcag agggtcgaac catcgcttca ttgcaatcaa tttagtgctg actatccccg
     4141 ggtctgttgc caacacccgt atgccctgcc ctgaaagccg ttctgccagt tccagactgt
     4201 acaccaaaat ggccctcttg gaacgggcat agctgaatat gctcataaac cgggcaccgg
     4261 gattgggtat gaacatgtct ttgttcaaac gaccgatgat ataagagcag gaaatggtat
     4321 ttatgataag tgaaccggga cgcatcaagg gcaaaagcag ttcattgagc aaaaacaacc
     4381 ccaggtaatt taccccaagg gttgtctcaa aaccgtcgcg agtaaggctg aaacgccccg
     4441 gcagcacgcc cgcattattg atcagggtat ttatgctgta ctttttccga gccagggtag
     4501 aagcaaattg cctgatagac gcgaatgaag ccagatccag attaagaagt tcgatttcgg
     4561 ctccgggatt gtcttgttcc aggatagctt taacagcagc cgacttttcc atattacgac
     4621 aagccataat aaggcgtatg ccacaaccgg ccaggctctg aactatagcc ttgcctattc
     4681 cggcagaggc tccggtgact acgataactt ctttcgatcc tgacattttc ggtttaaaaa
     4741 cgtcaaagat actcaaaaag caaaaaataa ggctgtctgc tttattttta tttcctttgt
     4801 acctaaaatt acccgataat gagaaataag cttcaattcc tggcaatact attgcagggc
     4861 ggccttctgc tgattttctg tgcaaaccac gttcaagccc gggcagagca agccgacgtc
     4921 agctttttgc agagcctgat caacgacagt caggttttgc tggcgcaggc tgtagaagga
     4981 caacaagagg ggaattatct cccgggatct aaacaagatt ttcaaacagc cctcgagcct
     5041 gcccttgcct tattgaacga cagcacggcc g
//
LOCUS       DAOSTD010000108         4976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_15369_length_4976_cov_14.831132, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000108 DAOSTD010000000
VERSION     DAOSTD010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15369_length_4976_cov_14.831132"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /locus_tag="PLI37_06865"
     CDS             <1..1442
                     /locus_tag="PLI37_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58764.1"
                     /translation="ARALREYDRAAVVASNLNRFFKDDEIEDQLQALSARLVSKSTAA
                     PKRDNCFVFYDHIGSNYVLALQYLRALMSWEAEILYILEPSRHSSSPPDFIKELKAYG
                     KANIIILPERTEDKLEHLNQVYCSIQEFGAAKALIHAPAEGAFCCVLWNALDELQRYR
                     IVPGDHHFYLGTRLSDYVIEFRDFGLALSYSRRAYKKEQLLCQPYYPIVNRAIPFEGF
                     PPQVKPNNIVVVSGGAMYKILGDGGRFLHLAKELLDYNKKVVLLYAGEGNTVKIKDFI
                     RKHKLEDRFILLGQRRDIYPLIKNSDIYLGTYPFSGGLMTQLAVVCEKPLLLLSYFPA
                     IRSADSLLNYGNKAQEPLSFYSVEAMLSYARQLIDDAAFRLKEGEKNRGRVISPQQFS
                     ESLRSLLNGETTIHFIPEMPEGLLERAEDLYLETADRYTKAYELFLFQSYGIKTLWLF
                     PKVFFKGLGSLSFIRRIVYTAVKRVTKKL"
     gene            complement(1426..2265)
                     /locus_tag="PLI37_06870"
     CDS             complement(1426..2265)
                     /locus_tag="PLI37_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ58765.1"
                     /translation="MSGIAILLTTCNSGKYLRPQLDSLFAQTMRDFEIHVHDDGSKDD
                     TLQILAEYQEKHPELILREDPQPGRLAKGSFTWLLENTQADYYMFCDHDDVWLDDKIE
                     KTYSQMRACEKAQPGKAVVVNTDLKVVDEQLNEIHPSFWAYNKIKPNLLKSFEYLTVC
                     NAFTGCTMMINRAAKELSLPMSEKALMHDKWIALKVAAAGGILESIHEATILYRQHGE
                     NQIGAREIGIAHYFKQLRSLPATWKENRATLEMSRAIKSFSWAKYLYYKFLYAIKRSK
                     VSS"
     gene            complement(2253..>3389)
                     /locus_tag="PLI37_06875"
     CDS             complement(2253..>3389)
                     /locus_tag="PLI37_06875"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOQ58766.1"
                     /translation="YVYNALNKHQHRKFALAVILLGVSSMIALYVYHPRFESIRPGKL
                     TESSSLQNKDPRLGIWFSGMQVLSENNRFLLGAGIGDVKDLLKEKYEQNRLSPEIIQN
                     NSHVHNTYLQALLETGIPGLLCLLTLLIAFPLNLRDKNERLYAGTIAFMFAMLMMVEV
                     VFLQIGGLIVFCLSFLFLSKAEGRKKERLSLPAGFCKTFTVINAAMVLMALVVFCYPL
                     YGKQGYDPKDPATYATDNYELIEDLPGDPPEELQDCWGYKISSTCRGYYEPHFDRMEF
                     LTGFYQPCPGEGRFSAWCYVSEDFDGVHVQIFTSSAYTGQGFSDEYDLSRKGSWQKLS
                     FDYSGVDKLYPMSLFFAKYRVKDFSTLEGYVVFALPLFELACQE"
     assembly_gap    3390..3489
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3490..3788)
                     /locus_tag="PLI37_06880"
     CDS             complement(<3490..3788)
                     /locus_tag="PLI37_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58767.1"
                     /translation="MPKITAFTGKFNYYFALLLAFYLSLQDRWLILVFFCWLVSWIID
                     VILSKRYLEFRFDKQHWTVYILWVSALLMLISVFYSTNLEYAYKVIERRVVIAAVP"
     gene            complement(3797..4882)
                     /locus_tag="PLI37_06885"
     CDS             complement(3797..4882)
                     /locus_tag="PLI37_06885"
                     /inference="COORDINATES: protein motif:HMM:NF026247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HOQ58768.1"
                     /translation="MAVYVIVIAVFLVLCLAGEEMKQNRQWLLLAMTLFLIAFAGFRG
                     NDVSKDHSIFQAFYDKALPFQTLFSNPGAFFKAAKVEPTFLILASFIKTYLPGWGFTF
                     LMVLFAFLSVTLKVKAIVKYSEFIFLSLFIYLCNTYMLHEIIQVRAGLAAALAMLAFE
                     PLVERKPWKFLLIMLLAILVHYSAIIFLPFYFLNTKKINPVVYIAVIVVPFLLSVFHF
                     DPISLLLKYDLGVYSEKLSIYITEQLRLKHAINKINLTIIFQLILSVFLLFYRTDLLE
                     KNKYSVLFLKINMISVGVFYLFFGLPVFAFRLWEMLNVVQIFLIPTLIYYIKPRWIPE
                     LIIVLVGIALYYNLVLQGHIREYSLIF"
BASE COUNT         1459 a         1082 c         1050 g         1285 t
ORIGIN      
        1 ttgcccgtgc attgcgggaa tacgatcgtg ctgccgtagt ggcttccaac ttaaatcgat
       61 tctttaaaga cgacgaaatc gaagatcaac ttcaagcctt atctgcccgc cttgtttcta
      121 aatcgaccgc tgccccaaaa cgcgacaatt gctttgtgtt ttacgatcat ataggctcca
      181 actatgtgtt ggctttgcaa tacctgaggg ctttgatgag ctgggaagcc gagatcctct
      241 atattttgga gcccagccgg cacagcagtt caccgcccga ttttatcaaa gagcttaaag
      301 catacggcaa ggccaacatt ataatcttgc ctgagcggac ggaagataag ttggagcatt
      361 tgaatcaagt ctattgcagc atacaggaat ttggggcagc taaagccctg atacacgcgc
      421 ccgcggaagg ggctttctgc tgtgtattgt ggaatgctct ggatgaattg caacgttacc
      481 gcatagtgcc gggcgaccat catttttacc tgggcacccg tctgagtgat tatgtcattg
      541 agttcaggga tttcggactc gcgttatcgt atagtcgcag agcttataaa aaggaacaac
      601 ttctttgtca accctattat cccatcgtta atcgggcaat tcctttcgag ggatttccgc
      661 ctcaggtcaa acccaataac atagtcgttg tttcgggtgg agccatgtat aaaatactgg
      721 gcgacggagg acggttttta cacctggcta aagaattact ggattacaac aagaaggtgg
      781 tgctgctcta cgccggcgaa ggaaatacag tgaaaatcaa agattttatt cgcaaacaca
      841 agttggagga tcgtttcatc ctcttgggac aacgccggga tatttatccc ctgattaaaa
      901 acagcgacat ctatttaggc acttatcctt tttcgggcgg attgatgact caactggcag
      961 tcgtatgcga aaagccgctt ttgctgctct cctattttcc tgccatcaga agtgcggaca
     1021 gtttgcttaa ttacgggaac aaagctcagg aaccgctttc gttttacagt gtagaggcaa
     1081 tgctgtctta tgcccggcaa ttaatagacg atgccgcttt tcgccttaag gaaggagaaa
     1141 aaaaccgcgg cagggtaatc agtccgcaac agttctcaga aagtttacgc tctttgctca
     1201 acggagaaac aacaatccat tttatacccg aaatgcccga aggattactc gaaagagccg
     1261 aagacttgta tttggaaacg gccgaccgct acaccaaagc ctatgaatta ttcctctttc
     1321 aaagctatgg cattaaaaca ctttggctct ttcccaaggt gtttttcaaa gggctgggtt
     1381 cattgagctt tatacgcagg atcgtttata cggccgtaaa aagggtcacg aagaaacttt
     1441 agacctttta atggcatata gaaatttata atacaagtat ttagcccaac taaaactttt
     1501 aattgcccgg ctcatttcca gtgtggcacg attttctttc caggttgcag gcagcgatct
     1561 gagctgttta aaataatgag ctatccctat ttcccgggct ccgatttggt tttcgccgtg
     1621 ctgccggtat aaaattgtgg cttcgtgtat ggattcgaga attccgccgg ctgctgctac
     1681 tttgagtgca atccatttat cgtgcatcag ggctttttca ctcattggta agcttagttc
     1741 tttggctgcc cggttaatca tcatggtgca gccggtaaag gcattgcaaa cggtcaggta
     1801 ttcaaagctt ttgagtaaat tcggtttgat cttgttatag gcccaaaagg agggatggat
     1861 ttcgttgagt tgttcatcca ccaccttcag atcggtatta accaccacgg ctttaccggg
     1921 ctgagctttt tcacatgccc gcatttggga gtaggttttc tcgattttat cgtccaacca
     1981 cacatcgtcg tggtcgcaaa acatatagta atcggcctgc gtgttttcca atagccaggt
     2041 aaaactgcct ttggccagtc tgccgggctg aggatcctcg cgcagtatca attcgggatg
     2101 tttttcttgg tattcggcca ggatttgcag ggtatcgtct ttggatccat cgtcgtgtac
     2161 gtggatttca aaatcccgca tggtctgggc aaacagcgaa tccaactgag ggcggaggta
     2221 tttcccgctg ttacaggtgg taagtagtat agctattcct gacatgctaa ttcaaaaagc
     2281 ggcaaggcaa aaacgacata gccttccagt gtactgaaat ctttaaccct gtacttggca
     2341 aaaaacaacg acatggggta caacttatcc actccggaat aatcgaaaga caacttttgc
     2401 cagcttcctt ttcggcttaa atcgtactca tctgaaaatc cttgtcctgt ataagctgag
     2461 ctggtaaaga tttgcacgtg aacaccgtca aaatcttcac tgacataaca ccaggccgaa
     2521 aatcttccct cccccggaca aggttggtaa aaaccggtta aaaactccat ccggtcgaaa
     2581 tgcggctcgt aatagccccg acaagtggat gaaatcttat aaccccaaca atcctgcagt
     2641 tcttcggggg gatcgcccgg caaatcctcg atcaattcat agttatcggt ggcgtaagtt
     2701 gccggatctt tcggatcgta gccttgtttt ccatagagcg gataacaaaa taccaccaga
     2761 gccattaata ccatggccgc attaataacc gtaaaagtct tacaaaaacc ggcaggtaaa
     2821 gacaagcgtt ctttcttgcg gccttctgcc ttggacaaaa acaaaaagct cagacaaaag
     2881 actatgaggc cgccaatttg caaaaaaacc acttccacca tcatcagcat agcaaacata
     2941 aaagcaatgg tgcctgcata caaacgctcg tttttatcgc gaagattgag tggaaaagca
     3001 ataagcaaag tcaataaaca gagcagtccc ggtataccgg tttccaataa tgcctgcaaa
     3061 taggtgttgt gcacgtggga attgttctga ataatttcag ggcttagccg gttctgttcg
     3121 tatttttctt tcagtaaatc tttcacgtca cctatgcccg ctccaagtaa aaaccgatta
     3181 ttttcactca aaacctgcat accggaaaac catatgccca gacggggatc tttgttttgc
     3241 agggaagagc tttcggtcag ttttcccgga cgtatggatt caaaacgggg atgatacaca
     3301 tacagggcta tcatggaaga aacgcccagc aatattaccg ccagggcaaa ttttcgatgc
     3361 tgatgcttgt tcagcgcatt atatacatan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnng gaacagcagc gatgaccact ctcctttcta tgactttgta ggcatattcc
     3541 agatttgtag aataaaagac agaaataagc atcaacagag cagagaccca taagatgtaa
     3601 actgtccaat gttgtttgtc gaagcgaaat tccaggtagc gtttactgag aataacatct
     3661 atgatccaac taaccaacca gcagaaaaac accaaaatca gccaacgatc ctgcaaactg
     3721 aggtaaaaag cgagcagtaa ggcgaagtaa tagttaaact tacccgtgaa ggctgtgatt
     3781 tttggcatac ccataactaa aaaatcaagg aatactctcg tatatgtccc tgaagaacaa
     3841 gattataata aagagctatc cccaccaaaa caataataag ttccggtatc cagcgaggtt
     3901 tgatataata gatcaatgtt ggaatcagaa agatttgcac tacgttcagc atttcccaaa
     3961 gcctgaatgc aaataccggc aggccaaaaa acaaataaaa aaccccaacg gaaatcatat
     4021 tgattttcag aaacaacacg ctgtatttgt ttttctcgag taaatccgta cggtaaaaca
     4081 atagaaaaac cgaaagtatg agttgaaata ttatcgtcag gttaattttg ttgatagcgt
     4141 gtttgagcct taattgctcc gtaatataga tgcttaattt ctcggagtaa actcccaaat
     4201 cgtatttcaa gagcaaagaa atcgggtcaa aatggaagac actcagcaaa aaagggacaa
     4261 caatgacagc tatatagact accggattta tttttttggt gttcaaaaaa tagaagggca
     4321 aaaatataat ggccgaataa tggaccaata ttgccaatag catgatcaac aaaaatttcc
     4381 agggttttct ttcgaccaaa ggttcgaaag cgagcattgc caaagctgcg gccaagcctg
     4441 cacgaacctg tatgatttcg tgcagcatat aagtattaca gagatatata aacagggaga
     4501 gaaatatgaa ttccgaatat ttgactatgg ccttgacttt aagagttacc gataaaaaag
     4561 caaacaacac catcaaaaag gtaaatcccc aaccgggcag ataggtttta ataaacgagg
     4621 ccaaaatcaa aaaagtgggt tccactttgg cggctttgaa aaacgccccc ggattactga
     4681 aaagtgtttg gaaaggcagg gctttgtcgt aaaaagcctg gaaaatgctg tggtccttgc
     4741 ttacgtcgtt accccggaat ccggcgaaag caattaaaaa cagagtcatc gccaacagca
     4801 gccattgcct gttttgtttc atctcctccc ctgcgagaca gagtaccagg aaaacagcaa
     4861 ttacaattac atagacagcc attctgaaaa atgaacaatt aacatccgga taagcctcat
     4921 cgggttaagg ccgaccttga aaacgcaaat gtactatttt tttgctcaat aagccc
//
LOCUS       DAOSTD010000109         4923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_15569_length_4923_cov_17.049712, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000109 DAOSTD010000000
VERSION     DAOSTD010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15569_length_4923_cov_17.049712"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1833)
                     /locus_tag="PLI37_06890"
     CDS             complement(40..1833)
                     /locus_tag="PLI37_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ58769.1"
                     /translation="MKKTANSLRKKTVVSFLLPAVRTSLFALICLLPALAFAQTADTK
                     ESEGQAYDTARSFSYYFEEGLRLKALEQYGAAFEMFRHCLSLDSTSAAAHAEIAAFYR
                     AMNQTERYVAHYEKAFALDPGNDWYGLQLAGIYQQLKRYPDAIRLLEQLVAKNPDNYE
                     LYYTLSLLHTELKQYTQAIEALDRFENSVGISQEISMQKYDLYLNLNQPKPAIKELQK
                     LQKSNPREIRYLLLVGSGWMEIGKTKKARKCFLQALDMEPENGTVRLFLSDYYKAVGD
                     SLAMNEQLHLALSNPKTELESKLNIFRTLLSTCENREDSLQIERYFELLLEQHPTAYD
                     LRDLRVRWLLLTQRKDEAVGELRNVLDLNPNQLRIWQDYLGLNLEEGNQEQIRRICLE
                     ALVYFPEEVEFWYYLALTYSMEENHSEALNAYQKCLEKADPKNRTFLSSILGYMGDIY
                     HQTGDTAEAYTHYKQALEYNPDNHLVLNNYAYFLSLDESDLSKAEKMSRRSLDFQPTN
                     STYLDTYAWVFFKQEQYRLAKIYIQRAIEYSKEPNIELLEHYGDILWFNNEKEAAVEQ
                     WKKASAMEGASDILQQKANRAVYLNKSSDEQ"
     gene            complement(1839..2273)
                     /gene="dut"
                     /locus_tag="PLI37_06895"
     CDS             complement(1839..2273)
                     /gene="dut"
                     /locus_tag="PLI37_06895"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOQ58770.1"
                     /translation="MTVKIVNRSKHPLPQYATSLSAGMDLRANIDAAVCIKPFERKLI
                     PTGLFIELPAGYEAQIRPRSGLALKKGLTVLNTPGTIDADYRGEIGVILINLSQEEVQ
                     VEDGERICQMVIASHCQATWQEVDTLEATERGAGGFGHTGKK"
     gene            2452..2967
                     /locus_tag="PLI37_06900"
     CDS             2452..2967
                     /locus_tag="PLI37_06900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOQ58771.1"
                     /translation="MKPAYLYLFLLGVLFSITSCLDDPEVTAWRKANDEFMLSLRDSS
                     DFKPLEYNDRILYGDENYNYLDHPVSGIYYKTLVEGRGAVPLVGQTVQVEYEGRLYDG
                     TVFDSGSRTTLVGTGVISGWSEVLYRIPQGSTWEVAIPYHLGYGEVKHGSIPAYSCLI
                     FTMELVSIVED"
     gene            complement(2974..3978)
                     /locus_tag="PLI37_06905"
     CDS             complement(2974..3978)
                     /locus_tag="PLI37_06905"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOQ58772.1"
                     /translation="MRLAIVGASGAVGQEFLKVLDERNFPMDDLVLFGSGRSAGKTFS
                     FRGKSIVVKELKHNDDFKGIDIAFTSAGASISKEFADTVTKFGTIMIDNSSAFRMDDD
                     VPLVVPELNAKDALQRPRNIIANPNCTTIQMVVALKPIEDLSHITKVHVSTYQAASGA
                     GAAAMDELIAQHTQLVKGETATVEKFAYQLAYNLIPQVDVFTENGYTKEEMKMFNETR
                     KIMHSDVLVSATCVRVPVLRAHSESIWVETERAISLEEARDAFARAQGIVLQDDPANK
                     SYPMPLFVSGKNPVYVGRIRKDLTNPKGLTFWAVSDQIKKGAALNAVQIAEYLLTQAR
                     "
     gene            4073..>4923
                     /locus_tag="PLI37_06910"
     CDS             4073..>4923
                     /locus_tag="PLI37_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOQ58773.1"
                     /translation="MHKCIFFSFSLLTIFFSCRPSVELQEEFGLFPVDTASAGSFYLS
                     LNEILFDPVPQSCDYVEIVNRSPVSVDLSEIRICNRNENGVLGSPKPLSRERFILDPG
                     AYCLLTSDWESLGRGFPLDTGLIFLIIKSLPSMPNDKGCVVLLDRQGNIIDECPYTKY
                     MHHCLISDREGIALEKIHPDLPSAEANSWLSAAADASYGTPGRINSQYRPLDTVGTNE
                     GFFVEQDWLSPLGDSKYKQLVLGYSFADRRVANIKVYNRSGALLYSLAENALLGARGF
                     FVWQGVD"
BASE COUNT         1141 a         1135 c         1246 g         1401 t
ORIGIN      
        1 aggtcagcag cagaagtaag aaactttttt gttgagttgt cattgttcgt cggatgattt
       61 gttcagatat acggcccggt ttgctttttg ttgtaagatg tcggaagctc cttccatggc
      121 agacgctttt ttccactgtt ccactgccgc ttctttttcg ttgttaaacc acagaatatc
      181 gccgtaatgt tccagtaatt caatgttagg ttctttggaa tactctatgg ccctttggat
      241 gtagattttg gccaggcggt attgttcttg cttgaaaaat acccaggcgt aagtgtccag
      301 ataagtgctg ttggtgggct ggaaatccaa acttctgcgg gacatttttt cggcttttga
      361 caaatcgctt tcgtcgagac tcaaaaaata ggcataattg ttcaggacta aatggttgtc
      421 gggattgtat tccagcgcct gtttataatg tgtgtaggct tcggctgtat cgcctgtttg
      481 atgataaata tcccccatat aacccagtat gctcgacagg aaagtcctgt ttttgggatc
      541 ggctttttcc aggcattttt gataggcatt cagcgcttcg ctgtggtttt cttccatgct
      601 gtaagtcagg gccaggtaat accagaattc tacttcttcg gggaaataca ccaatgcttc
      661 caggcaaatc cggcgaatct gttcctgatt gccttcttcg aggttgaggc ctaaataatc
      721 ctgccagatg cgtaattgat tgggattcaa gtcgagtaca ttgcgcaatt caccgacggc
      781 ttcgtctttg cgttgggtga gcagcaacca gcgaaccctt agatcgcgca gatcgtaggc
      841 cgtgggatgt tgctcaagca ggagttcaaa atagcgctca atctgcaggc tgtcttcccg
      901 gttttcgcag gtactgagca gagttctgaa gatgttcagt ttgctctcca gttcggtttt
      961 tgggttgctt agggcaaggt ggagctgttc gttcatggcc agactgtcgc cgacggcttt
     1021 gtaataatcc gacaaaaaca gccgcacggt gccgttttcg ggctccatgt cgagggcttg
     1081 gaggaagcat ttcctggctt ttttggtttt tccgatttcc atccagccgc tacccaccag
     1141 caaaagatat ctgatctcgc gggggttgga cttttgaagc ttttgcagtt ctttgatggc
     1201 cggtttgggt tgattcaaat tcaggtacaa atcgtatttc tgcatgctga tttcctggct
     1261 gatgccgacg gaattttcaa aacgatccag ggcctcgatg gcttgagtgt attgtttaag
     1321 ctcggtgtgg agcagcgaca gcgtataata caattcgtaa ttgtcgggat ttttggctac
     1381 cagttgttcc agcagtctta tggcgtccgg ataacgtttc agttgctggt aaatgccggc
     1441 cagttgcaag ccgtaccaat cgttgccggg atccagagca aaagcttttt cgtaatgagc
     1501 cacataacgc tcggtttgat tcatagcccg gtagaaagcg gcaatttctg cgtgagccgc
     1561 cgcactagtg gaatccagac tgaggcaatg cctgaacatc tcaaaggcgg ctccgtattg
     1621 ttccaacgct ttcagtcgca ggccttcctc aaaataatag ctaaaactgc gggctgtgtc
     1681 gtaagcttgt ccctcgcttt ctttagtatc ggcagtttgg gcaaaggcaa gtgcaggcag
     1741 caggcaaatc aatgcaaaca agcttgtcct gactgccggc agtaaaaatg aaacgaccgt
     1801 ctttttacgt agtgaattag ccgtcttttt catcccattt attttttccc cgtgtgaccg
     1861 aaaccaccgg cacctctctc cgtggcttcg agtgtgtcta cttcttgcca ggtcgcctga
     1921 caatgagagg caatcaccat ttgacagatt ctttctccgt cttcgacctg tacttcctct
     1981 tgcgacaaat tgatcaaaat aacccctatt tcgccccggt agtcggcatc aatagtgccg
     2041 ggagtgttca gtacggtgag tccttttttg agggccagac cgctgcgggg cctgatttga
     2101 gcttcgtaac cggccggcag ttcaataaaa agtccggtag gtatcagctt gcgttcgaaa
     2161 ggtttgatgc agacagcggc atcgatgttg gcgcgcaggt ccatgcctgc cgaaaggctg
     2221 gttgcgtatt ggggcagcgg gtgtttggat cggttgacta tctttacggt catatcttta
     2281 tggagactta aattgctgta aattcaataa atagcttaaa agtacattta aacggacaaa
     2341 agtactaaaa ttattgacag gccgaataag agcttttaaa aaagcaggat tgtccgggga
     2401 atttcttaca tttgcgggct gtttgcccgg cagcatttta atcgaaccaa tatgaagcct
     2461 gcttatttgt atctgttcct gctcggagtg cttttttcga tcacttcctg tctcgacgat
     2521 cctgaagtca ccgcctggcg caaagctaat gacgagttta tgctaagcct cagggattcc
     2581 tcggacttta agcctttgga atacaacgat cgtattttgt acggcgacga gaactacaat
     2641 tacctggatc atcctgtgag cggtatttat tacaaaaccc tggtagaggg ccggggtgcg
     2701 gtgcccttgg tcggacagac ggtacaagtg gagtacgaag ggcgcttata cgacggcact
     2761 gtttttgaca gcggcagcag aaccacgctg gtaggtaccg gagtcatcag cggctggtcg
     2821 gaagtgctgt acagaatacc gcaaggttcc acctgggagg tggccattcc ttatcacctg
     2881 gggtacggag aggtaaaaca cggttccata ccggcttact cctgtcttat tttcacaatg
     2941 gagttagtca gcatagtcga agactaaaag caatcaacgt gcctgagtga gtaggtattc
     3001 tgcaatttgt acggcgttca gggcagctcc tttttttatc tgatcactta cagcccagaa
     3061 agtcagtccc ttaggattgg ttaggtcttt tctgatgcgt cccacgtaaa ccgggttctt
     3121 gccggaaaca aacaagggca tgggatagct cttgttggcc ggatcgtcct gcaaaacaat
     3181 accctgggcc cgggcaaaag catccctggc ttcttcgagg ctaatggcgc gctctgtttc
     3241 gacccagata ctttcggaat gagcacgcaa aaccggtaca cgcacacaag tggcgcttac
     3301 caacacgtcg gaatgcataa ttttccgggt ttcgttgaac atcttcattt cctctttggt
     3361 atagccgttt tcggtgaaaa catccacttg aggaatcagg ttgtaggcca actgataggc
     3421 aaatttttcc acggtggcgg tttcgccctt taccaactgg gtgtgttgag caatcaattc
     3481 atccatggca gcggcaccgg ccccgctggc agcctgatag gttgacacat gcacttttgt
     3541 gatatgactc aggtcttcga tgggtttgag ggctaccacc atttgtatgg tggtgcaatt
     3601 cggattggca ataatatttc ggggtctttg caaagcgtct tttgcgttca gttcaggcac
     3661 gaccagcggc acatcgtcgt ccatgcgaaa agcgctggag ttgtcgatca taatagtgcc
     3721 gaatttggta acggtgtccg caaattcttt tgaaatactt gctccggccg aagtgaaggc
     3781 tatgtctatt cccttgaagt cgtcgttatg ttttagctcc ttgaccacga ttgattttcc
     3841 gcgaaaagaa aaggttttgc cggcactacg tccggatccg aacaacacca ggtcgtccat
     3901 ggggaaatta cgttcatcaa gtactttgag aaattcttgt cctacagcgc cgctggcgcc
     3961 aacaattgca agtctcatgt taaaataaaa ttaataaaag gataatataa tacataaaat
     4021 gcttaccttt gccgctgaat tcatgccacg caaaggtagt aaaaaagtga agatgcacaa
     4081 gtgtatattc ttttcattta gtctgctgac gattttcttt tcctgcagac cgtcggtgga
     4141 actgcaggaa gaattcggcc tttttcccgt cgataccgct tcggccggct cgttttatct
     4201 gagcctgaac gagatattgt tcgacccggt accgcaatct tgtgattacg tcgaaatcgt
     4261 caaccgttct ccggtctccg tagatctgtc cgaaatacgt atctgcaacc gcaacgaaaa
     4321 tggggtactg ggcagtccca aacccctgag ccgtgagcgt tttatactcg atccgggtgc
     4381 ttattgtttg ttgacctccg attgggaaag tcttggaagg ggattcccac tggatacagg
     4441 gttgattttt ttgattatca aaagcttgcc ttccatgccc aacgacaaag gctgtgtggt
     4501 tttgctggac cggcagggaa acatcataga cgaatgccct tacacaaaat atatgcatca
     4561 ctgcctgata agcgataggg agggtattgc tttggagaag atacatccag atttaccttc
     4621 ggcagaagcg aattcctggc tttcggctgc ggccgatgcc tcttacggaa cgcccggcag
     4681 gatcaattct cagtaccggc ctctggacac cgtcggtaca aacgaaggtt tttttgtgga
     4741 acaggattgg ttgtcgccct tgggagattc aaagtacaaa cagttggtgt tgggctactc
     4801 tttcgccgat cggcgcgtgg ctaacatcaa ggtttacaac cgcagcgggg ccttgcttta
     4861 ttcgctggca gaaaatgccc tgctcggagc ccggggcttt ttcgtctggc agggagtcga
     4921 cag
//
LOCUS       DAOSTD010000110         4770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_16230_length_4770_cov_14.000424, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000110 DAOSTD010000000
VERSION     DAOSTD010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16230_length_4770_cov_14.000424"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /gene="ricT"
                     /locus_tag="PLI37_06915"
     CDS             complement(<1..1013)
                     /gene="ricT"
                     /locus_tag="PLI37_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOQ58774.1"
                     /translation="MSTKACGCNKIKKLNTFDWLCDLSEAQASTDYVEVQFKNTRKGY
                     YLNSNKIPLQKGDLVAVESSPGHDIGEVSLTGHLVLKQMKKINYRFDQQEVRRIYRKA
                     RPVDLEKFAESKALEHDTMLRSRKIAEELNLNMKIGDVEYQGDGNKAIFYYIADDRVD
                     FRQLIRVLADTFHVRIEMKQIGARQEAGRIGGIGPCGREMCCSSWMTNFVSVSTSSAR
                     YQDLSLNPQKLAGQCSKLKCCLNYETDMYVEAQKGFPAKDIVLETKDASYFQIKTDLL
                     KRQVSYSTDKDSMVNAISIPVERALQIIALNKKGIRVDSLLELAEEKSRNEFGDVLNS
                     DLQR"
     gene            complement(1097..2257)
                     /locus_tag="PLI37_06920"
     CDS             complement(1097..2257)
                     /locus_tag="PLI37_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOQ58775.1"
                     /translation="MYFKDVIGQQAVKKRLLDMVDTGRMPHALMLSGNPGRGKLALAL
                     ALARYICCTDRQTGDSCGQCASCRKFNKLVHPDLHFVFPIYKPDSKKRWICDDFLPQW
                     REMAAGKPYFSYNSWMEYIGAGNAQGMIYAEESSEILRKLSFKSIEAEYKVMLIWLPE
                     KMHTSCANKLLKILEEPQGNTVFLLVSENSEKNLETIYSRAQHIQVPRIDDEDMERAL
                     QELKAMEPASLKSLVRLAGGSWLEALRLLESDEEDRFFLEQFIRNMRAAYTIAHFPPS
                     KLPEKQKSLQDLRLWSEEMAKIGRERQKNYLSFAQRMVRENFILNTREEKLSYLNPEE
                     MSFSSKFFPYINRRNVQAFMDLFALAERHIEQNVQAKMVFFDTAVQSILLFK"
     gene            complement(2247..3539)
                     /locus_tag="PLI37_06925"
     CDS             complement(2247..3539)
                     /locus_tag="PLI37_06925"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOQ58776.1"
                     /translation="MKQALQIFSRLAIVLTLLTATQGCRQKSQTDKTTDSTEIHTQEE
                     DPARYFLGIRTDTLAMQEGKIPRGGSLSFLLGQLGVGPSTIDQCAKKARPVFNVRHLQ
                     AGKPYYSLSSKDSTNTLKYFIYQENVTDFVVFDFSDSLQIYRKQKEISVETRLAEGEI
                     DHSLWMSMKERGYNINLALDLSDIYAWQIDFFGIQAGDSYKVIYDEHFVDDTVSVGIG
                     KIHGAVFNHVGHDYYAIPFVQDGFRDFFDDRGINLRKAFLKAPLRYSRISSRYSHSRM
                     HPVLRIRRPHHGVDYAAPTGTPVYSIGAGVVVKKAYQAGGGGNYVSVKHNSTYTSTYM
                     HLSGFAKGLAVGQKVSQGELLGYVGSSGLSTGPHLDFRVYKNGNPIDPLKMESPPSTP
                     LKPEYKDSFELVKLEIIRQLQAEPLIASADTTKTENVF"
     gene            3759..4760
                     /gene="gap"
                     /locus_tag="PLI37_06930"
     CDS             3759..4760
                     /gene="gap"
                     /locus_tag="PLI37_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOQ58777.1"
                     /translation="MIKVGINGFGRIGRLVFRAAQERNDVQIVAVNDPFMDLDYVIYM
                     LKFDTVHGRFKGTAENKDGKLVVNGKEVAFFAEKNPADIPWGKVGADYIVESTGVFTK
                     LEGASEHLKAGAKKVVISAPSADAPMFVCGVNLDKYTKDMNVVSNASCTTNCLAPLAK
                     VIHDNFGIVEGLMTTVHAATNTQKTVDAPSKKDWRGGRSILNNIIPSSTGAAKAVGKV
                     IPDLNGKLTGMAFRVPVADVSVVDLTVRLKKAASYEEIKAAVKKACQTNMKGIMDYTE
                     DSVVSTDFIHDPHTSIFDANAGIALNDNFVKLVSWYDNEWGYSCKTLDLIVHMDSVK"
BASE COUNT         1111 a         1089 c         1203 g         1367 t
ORIGIN      
        1 cgttgcagat cgctgttgag gacatcgcca aattcgttcc ggcttttttc ttcggccagt
       61 tccagcagtg agtcaactcg gatgcctttt ttattcagcg caataatctg cagggctctc
      121 tccaccggta tgctgatggc gtttaccatg gaatctttgt cggtggagta gctgacctga
      181 cgttttaaga ggtcggtctt gatctgaaaa tagctggcgt ctttggtttc gagcactatg
      241 tctttggccg ggaaaccctt ttgagcctcc acatacatat ctgtttcgta gttgaggcaa
      301 catttgagtt tgctgcattg tccggccaat ttttgagggt tcaggctcag gtcctggtaa
      361 cgggcagagc tggtagatac ggaaacaaag ttggtcatcc aggatgaaca gcacatttct
      421 ctgccgcagg ggcctatgcc tcctatacgg ccggcctcct gacgggctcc gatctgtttc
      481 atttctatac gcacatggaa ggtgtcggcc agtaccctta tcagttgcct gaagtccacc
      541 cggtcgtcgg cgatatagta aaaaatggct ttgtttccgt cgccctggta ttctacgtcg
      601 cctatcttca tgttgagatt gagctcttcg gctattttgc gggaacgcag catggtgtcg
      661 tgttcaaggg ctttggattc ggcgaatttt tcgaggtcta cgggacgggc tttgcggtag
      721 atccggcgga cttcctgctg atcaaagcga taattgattt ttttcatctg cttgagcacc
      781 agatggccgg taaggctcac ttcgccgatg tcgtgacccg gcgaagattc caccgccacc
      841 aaatcgcctt tttgcagggg gattttgttg ctgttcaggt aatatccctt gcgggtattt
      901 ttgaattgca cctcaacgta atccgtcgaa gcctgagctt cggacaaatc gcagagccaa
      961 tcgaaagtgt tcagtttttt tattttgtta caaccgcaag ccttggtaga catcaagaag
     1021 ccgttgtttt gtagtttata ggtcataaaa agcttattta agctacaaaa gtaatttttt
     1081 ttagcttcta acgcaattat ttaaacaaga ggatgctttg cacggcggta tcgaaaaaca
     1141 ccattttggc ctgtacgttc tgttcgatat gccgttcggc cagagcaaag agatccataa
     1201 aagcctgaac gttgcgccgg ttgatgtagg gaaaaaactt ggaagaaaaa gacatttctt
     1261 cgggattgag gtagctgagt ttttcttcgc gggtgttgag gatgaaattt tctcgaacca
     1321 tgcgttgggc aaaactcagg taattctttt gtcgttccct gcctattttg gccatctctt
     1381 ccgaccaaag cctgaggtct tgcaggcttt tttgcttttc gggcaatttg gaagggggaa
     1441 aatgggcaat ggtataagct gcccgcatgt tgcgtatgaa ttgctccagg aagaaacggt
     1501 cttcttcgtc cgattccaat aaacggagtg cttcgagcca actgcctccc gccagacgaa
     1561 caagcgattt gaggcttgcc ggttccatag ctttgagctc ttgcaaggcc ctttccatat
     1621 cctcgtcgtc gatgcggggc acttgaatat gctgagccct cgaataaatg gtttccagat
     1681 ttttttcgct gttttccgat accaaaagaa atacggtgtt accttgaggt tcctccagta
     1741 ttttgagcaa tttgttggcg caggaagtgt gcattttttc gggcaaccag atcagcatta
     1801 ccttatattc ggcttcgatg ctcttgaagc tgagtttgcg caaaatctcg gaactttctt
     1861 cggcgtagat cataccctgg gcattgccgg ctccgatgta ttccatccaa ctgttgtagc
     1921 taaagtaggg tttacctgcg gccatttccc tccactgcgg gagaaaatcg tcgcagatcc
     1981 accttttctt actgtcgggt ttataaatgg gaaacacaaa atgcaaatcg ggatgaacca
     2041 gcttgttgaa tttgcggcag gaagcacatt gtccgcagga gtcgcctgtc tgccggtctg
     2101 tgcagcaaat ataacgggcc agagccagag ccaaggccag cttacctctg ccgggattgc
     2161 ccgacaacat cagggcatgg ggcatacggc cggtgtcgac catatcgagc aaacgttttt
     2221 tgacggcctg ctggcctatg acatctttaa aatacatttt ccgtttttgt tgtgtctgca
     2281 ctggctatca aaggttcagc ttgcaattgc cggatgatct cgagtttgac cagttcaaaa
     2341 ctgtctttat attcgggttt caatggggta gaggggggcg attccatctt gagcggatcg
     2401 atggggttgc cgtttttgta caccctgaaa tccagatgag ggccggtaga gagcccgctg
     2461 gagcccacat aacccagcaa ttctccctgg gaaacttttt ggcctaccgc caggcccttg
     2521 gcaaaaccgc ttaggtgcat ataggtgctg gtataggtgc tgttgtgctt aacgctgacg
     2581 taattgccgc cgcctccggc ctgataggct ttttttacca cgacgccggc accgatgctg
     2641 tacacgggag tgcccgtagg agcagcgtaa tccactccgt gatgcggtct gcgaattctt
     2701 agtaccggat gcatccggct gtggctgtag cgcgaactga tgcgggaata acgcaaagga
     2761 gccttcaaga aggctttacg caggttgatt ccccggtcgt cgaaaaaatc cctaaatccg
     2821 tcctgcacaa agggaatggc gtaataatcg tgacccacgt gattaaaaac ggctccgtgt
     2881 attttgccta tgcccaccga caccgtgtcg tcgacaaaat gctcgtcgta gattaccttg
     2941 tagctgtcgc cggcctgtat gccgaagaag tcgatttgcc aggcgtaaat atccgaaagg
     3001 tccagggcca ggttgatatt atatccccgt tctttcatgc tcatccacag ggaatggtcg
     3061 atttcaccct cagccagccg ggtttcgacg ctgatttctt tttgtttgcg gtaaatctgc
     3121 aggctgtcgg aaaaatcgaa gaccacaaaa tcggtgacgt tttcctgata gatgaaatat
     3181 ttcaatgtgt tggtgctgtc tttgctgctc agactgtaat aaggcttgcc tgcctgcaga
     3241 tgcctgacgt tgaataccgg tctggccttt ttggcgcatt ggtctatcgt cgagggaccg
     3301 acgcccagtt gacccaataa aaacgaaagg ctgcctccac ggggaatctt accctcttgc
     3361 atagctaagg tatctgtgcg gatacccaga aaataacggg cgggatcttc ttcttgggtg
     3421 tgaatctcgg tactgtcggt tgttttatcg gtttggcttt tttgccggca gccctgtgta
     3481 gctgttagca gcgttagcac gatggcgagc cggctaaaaa tctgaagcgc ttgtttcatc
     3541 gggtttatcc ttgtatttga aagggacaaa ttaaaagaat tttatcggaa actactctct
     3601 ttgcccggtc tattttaaac aagctaaagt tttgttttaa attctttggt tttgttgccg
     3661 gtaaaatgat aaaatatacc tcgaaacagg tgtgtttttg tggaaaaaga gtatctttgt
     3721 tgccaaattt aagttttact ctaaaaaaca aaacaaacat gattaaagta ggtattaatg
     3781 gttttggccg catcggccgt ttggtttttc gcgccgctca ggaaaggaac gacgtgcaga
     3841 tagtagcagt gaacgatcct tttatggatc tggactacgt catctatatg ttgaaattcg
     3901 atactgtaca cggacgtttc aaaggtacag ccgaaaacaa ggatggtaaa ttggtggtga
     3961 acggtaagga agtggctttc ttcgccgaaa agaatccggc cgacattccc tggggaaagg
     4021 taggcgccga ttacatagtg gaatcgaccg gtgtatttac caaactcgaa ggcgcttcgg
     4081 aacacctgaa agccggtgcc aagaaggtgg ttatttctgc tccttcggcc gatgctccca
     4141 tgtttgtttg cggtgttaac ctggacaaat ataccaaaga tatgaacgtg gtttccaatg
     4201 cttcttgcac cacgaactgt ctggctccct tagccaaagt tattcacgac aatttcggta
     4261 tcgtggaagg cctgatgact actgttcacg ctgctaccaa tacgcagaaa acggtggatg
     4321 ctccttccaa gaaagactgg agaggcggcc gttccattct gaacaatatt attccttctt
     4381 ctaccggtgc tgccaaagcg gttggtaagg ttattcccga tctgaacggt aaactgaccg
     4441 gtatggcttt ccgtgttcct gttgccgacg tttcggtggt cgatttgacc gttcgtctta
     4501 agaaggctgc ttcttacgaa gaaatcaagg ctgctgttaa gaaggcttgt cagaccaaca
     4561 tgaaaggtat tatggattat accgaagact ctgtggtttc gaccgacttt atccacgatc
     4621 cgcatacttc cattttcgat gccaatgccg gtattgcttt gaacgacaat ttcgtcaagc
     4681 tggttagctg gtacgacaac gaatggggtt attcctgcaa gaccctggat ttgattgttc
     4741 atatggacag cgtgaaataa atttgcgcta
//
LOCUS       DAOSTD010000111         4737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_16376_length_4737_cov_14.219351, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000111 DAOSTD010000000
VERSION     DAOSTD010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16376_length_4737_cov_14.219351"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLI37_06935"
     CDS             complement(<1..323)
                     /locus_tag="PLI37_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOQ58778.1"
                     /translation="MEKHNAVLLLCTGGTISMSEDPVTGALRPVNFEKIFDYLPELNK
                     IGIRTDSIAFDPLIDSSDVDPSIWSKLAHIIYDHYHQYDGFVVLHGTDTMAYSASALS
                     FMLNNL"
     gene            453..>1453
                     /locus_tag="PLI37_06940"
     CDS             453..>1453
                     /locus_tag="PLI37_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOQ58779.1"
                     /translation="MLRFASAALKTALSLLLFLLLPVLAAKAETKVLINEFLSINSGI
                     IQDEDGDNSDWIELYNAGEEAVNLENWSLTDDSTNLQKWLFPSVNLEAGAYLLVFASD
                     KDRRTENEELHTNFKLASSGEFLALVEPGALVPAFAYSPAYPPQRSNVSYGIYQGQLC
                     YLENPSPGSENLLGDQVLPPVFSKPAGFYQRAFELELSVALEGLDIYYTTDGSLPTKE
                     QAQLYTTPLSITTTTVVRAVCITGDERVSPSVSRSFIFLEDVLRQGQQPVGYPQVWND
                     STYPADYEMDAGICQQPDYENRILDALLELPSISIMLHPDSLFSLDRGIYVNSEKKG"
     assembly_gap    1454..1553
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1554..3187)
                     /locus_tag="PLI37_06945"
     CDS             complement(<1554..3187)
                     /locus_tag="PLI37_06945"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOQ58780.1"
                     /translation="MNKALLFLVLCALAGTAKAAVDYQVRISEFMASNKSTILDSEGK
                     SSDWIEIHNAGSEAVTLEDWSLSDERKNPRKWVFPAVEIKAGAYLLVFASGKNRRDPG
                     EELHCNFKLSSAGEYLALFGPEGVAYSCFDTYPAQEADVSYGYIDSCLFFFEQATPGH
                     PNEQSIAIRLLSPVCSHPRGYYDTAFVLTLESLVPGADIYYTLDGSPPIPGHSTLYEQ
                     TLSVTGNTILRAVCAKENYLPSKAATLSYLFIDDILNQSNSPEGYPEFWGSYAQKTGT
                     AVADYEMDPELIAEPAYAASVREGLRDLPVVSIAGDKNHFFSHEKDSVNGGIYIYTGP
                     PVGKDKTGDGWERPVSFEYFNSADSVSLQVNCGIRIHGGHSRLAEKSPKHSFRLVFKP
                     KFGPTRLDFPFFGPKGPEELNALVLRAGFCNSWIHSDAEARTIAQYIRDAWAKNTQQQ
                     MGHPASCNSYAHLFINGLYWGLYNPSERIEDDYCEIHFGGDKEDYDVIKQDEEQNNII
                     YASDGNTTAWFKLISALKTTPGQAVYQEIQGKNPDGS"
     assembly_gap    3304..3403
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3404..>4737)
                     /locus_tag="PLI37_06950"
     CDS             complement(<3404..>4737)
                     /locus_tag="PLI37_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58781.1"
                     /translation="YALEDYSIGIFFRVDESYGNLSAYGNWVWNFSNSDQLATEANGA
                     MWGALRSQQQTICKTHYGTEQTVQMGSPAPQGKWHHMAATYNKETQTATLYIDGIAVA
                     TNNRVSQTCANALAISGRSGTLYNWLGRSCYTGDAYLAQTLLYDFQLHRVTLSQTELN
                     NHFSITNTLMALNAAYEENPDYHNPLLPQEFEALSLVDTLDQDLTLPVQGRIDPGISI
                     KWSSSHPEILSNEGVVNRPDYVDFIVSLTATLQSGADYMNKTFRVVVKANEGSSFKGN
                     LILHHDFSVRVDSLVKDRAEKQFDAVLKGGAQVRKIGLNDNSFKVLDLGKSQTAYLDL
                     GEEIGKIIPSLSDFSIACYYRIDKEYQDLNWNGNCLWNFSNSDDIYTEPLGYMSAFLN
                     NQGYYISSGNWISEQSLTTGSTAGKDSWHHLTYTQQANTGTLYLDGLMVASG"
BASE COUNT         1088 a         1000 c         1157 g         1292 t
ORIGIN      
        1 aggttgttca gcataaagct cagagccgaa gccgaataag ccatagtgtc tgtcccgtgc
       61 aataccacaa aaccgtcgta ttgatgatag tgatcataga tgatatgtgc cagtttgctc
      121 cagatcgaag gatcaacatc ggaggagtct ataagcggat caaaagcaat ggaatccgtc
      181 ctgattccga tcttgtttag ttcgggcagg taatcgaaaa ttttttcaaa attgacaggt
      241 cgcagcgccc cggtaacagg atcttcagac atgctgatgg tgccgccggt acagagtagc
      301 aatactgcat tatgtttttc cacggctgtc gttttatgta cgcaaaaata gacaattagg
      361 aacaattaca aaattttgcc tatttttgcc ggcttaattt ttgatttttg taccgcttga
      421 ataactctga ctcagatttt tttctctttc gtatgctgcg atttgcatct gctgctttaa
      481 agacggcttt gtctttatta ttgtttcttt tgttgccggt ccttgctgcc aaagctgaga
      541 ctaaggtgct tatcaatgaa tttttgagta ttaacagcgg cattattcaa gatgaggacg
      601 gtgacaattc cgactggata gaattgtaca atgcgggaga agaggctgtc aatctggaga
      661 actggtcttt gaccgatgat tcgacgaatt tacagaaatg gctgttcccc tccgtcaatc
      721 tggaagccgg agcctatttg ctggtttttg cttcggacaa agacaggagg acggaaaacg
      781 aagaattgca taccaacttt aaattagcct cttcgggcga gtttctggcc ctggttgagc
      841 cgggagcttt ggttcctgcc tttgcttatt caccggcata tccgcctcag cgaagcaatg
      901 tgtcttatgg tatctatcag gggcaattgt gttatctcga aaatcccagt ccgggttccg
      961 aaaatttact cggagatcag gtgttgccgc cggttttcag caagcctgcc ggcttttacc
     1021 aaagggcttt tgaattggag ctgagtgtgg cgctcgaggg cctggacatt tattatacta
     1081 ccgacggtag tttgcctacc aaagaacaag ctcaactgta taccacccct ctgtcaatca
     1141 ctactacgac agttgtccgg gctgtgtgca taaccggaga tgagcgcgta agcccttccg
     1201 tgagcagatc atttattttt ttggaggatg tactcaggca aggtcaacag cctgtcggtt
     1261 atcctcaggt atggaacgac agtacttatc cggccgatta cgagatggat gccggtattt
     1321 gccaacaacc cgattacgag aatcgaatac tcgatgcctt gcttgagtta cccagcatca
     1381 gtataatgtt gcatcccgat tccctgttta gtttggatag gggtatttac gtgaattccg
     1441 agaaaaaagg agannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngagccgt
     1561 cgggattttt gccttgaatt tcctgataga cggcttgtcc gggggtggtt ttaagggcgg
     1621 agataagctt gaaccaggct gtagtgtttc cgtcggaagc ataaattata ttgttttgtt
     1681 cctcatcttg ttttataaca tcgtagtcct ctttgtctcc cccgaaatgt atttcgcaat
     1741 aatcgtcttc aattctttcg gaaggattgt acaagcccca gtacaggccg ttgataaaga
     1801 gatgtgcata ggaattgcaa ctggcaggat gtcccatttg ctgttgggtg ttcttggccc
     1861 aggcatcgcg gatgtattgc gcaatggttc tggcttcggc atcggaatga atccagctgt
     1921 tgcaaaaacc tgctctcaat accaaagcgt tcaattcttc aggcccctta ggtccgaaaa
     1981 aaggaaaatc caaacgggtc gggccaaatt tgggcttgaa aaccaaacga aaagaatgtt
     2041 tgggactttt ttcggccaga cggctatgtc ccccgtgtat gcgaatgccg cagttaactt
     2101 gcagagaaac gctatcggcg gagttgaaat attcaaaaga aaccggcctt tcccatccgt
     2161 ctccggtttt gtccttgccc acgggtggcc cggtatagat gtaaatgcct ccgtttacag
     2221 agtctttttc gtggctgaaa aaatgatttt tatcgcctgc tatcgaaacg acgggcaaat
     2281 cgcggagccc ttcgcgtacc gaggcggcat aggcaggttc ggctatcaat tcaggatcca
     2341 tttcgtaatc ggcgacggca gtacctgttt tttgagcata gctgccccaa aactccggat
     2401 agccttccgg gctgttggat tgattcagga tatcgtcgat gaacaaataa ctcagtgtgg
     2461 cggccttgct ggggaggtaa ttctctttag cgcaaacagc ccttagaatg gtgttgccgg
     2521 tgacggatag ggtctgctcg tacagtgtac tatggcccgg tataggcggg ctgccgtcca
     2581 gtgtgtaata aatatcggct ccggggacaa gggattccaa agtcagcaca aaggccgtat
     2641 cgtaatatcc cctgggatga ctgcaaaccg gagaaagcag acgtatggca atcgattgtt
     2701 cattgggatg tccgggagtg gcttgttcaa agaaaaaaag gcaactgtct atgtaaccgt
     2761 aagagacatc ggcttcctgg gccggatagg tatcgaaaca actgtaggca actccctcag
     2821 gaccgaacaa tgcgaggtat tcgcctgcgc tgcttaattt aaagttgcag tgcaattctt
     2881 ccccgggatc tcttctattt ttgccggaag caaagaccag caaataggct ccggctttta
     2941 tctcaacagc gggaaatacc catttacggg gattttttcg ctcatcgctc agagaccagt
     3001 cttcgagcgt tacggcctca ctgccggcgt tgtgaatttc tatccagtcg gaggattttc
     3061 cttcggaatc gaggatggtc gatttattgg aggccataaa ttccgaaatt cttacctgat
     3121 aatcgacggc agctttcgca gtgccggcta gggcgcacaa gactaggaac agtaaggctt
     3181 tgttcataaa gcaggattag gaatgatgac ttttgtcgtc catacacagg tttgctcgcg
     3241 aataagctgt aataaataca taccggtttg aaactccgaa ctgtttatgc taatgctgtt
     3301 accnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcccgag gcgaccatca
     3421 ggccatcgag gtataaagtg cctgtattag cttgttgtgt gtaggtcaaa tgatgccagg
     3481 agtctttgcc ggccgtactg cctgttgtga ggctttgttc cgatatccaa ttgccggagg
     3541 aaatataata accctgattg ttcaaaaagg ccgacatata tcccagaggt tcggtatata
     3601 tatcgtcgga gttggagaaa ttccacaggc agttgccgtt ccaatttaaa tcctgatact
     3661 ctttgtctat tctgtaatag caggcaatac tgaaatcgga gaggctgggg atgatttttc
     3721 cgatttcttc gcccaggtcc agataagctg tttggctttt acctaaatca agcaccttga
     3781 agctgttgtc gttcaatccg atttttctta cttgagctcc tccttttaaa acggcgtcaa
     3841 attgtttttc tgcccggtct tttaccaggc tgtcgaccct tactgaaaaa tcgtggtgaa
     3901 gtataaggtt tcctttaaaa gagcttcctt cattggcctt gactaccaca cgaaatgttt
     3961 tattcatgta atcggctcca ctctggagag tagccgttag gctgacgatg aaatcaacgt
     4021 aatcggggcg attaactacg ccttcgttgg agaggatttc aggatggctc gaactccact
     4081 taatgctgat accggggtca atccggccct gaacgggtaa ggttaggtct tgatcgagag
     4141 tgtctaccag gctcagggct tcgaattcct ggggcaaaag gggattgtgg taatcgggat
     4201 tttcttcgta agctgcgttg agtgccataa gggtgtttgt aatactgaag tgattgttca
     4261 gttcagtctg ggataaagtt accctgtgca gttgaaaatc gtagagcagg gtttgggcca
     4321 ggtaggcatc tccggtatag caggagcgtc ccagccagtt gtataaagtg ccgctgcgtc
     4381 cgcttattgc aagggcattg gcacaagttt ggcttacccg gttgttggtg gccacagcga
     4441 ttccgtcgat gtaaagtgtg gcggtttgcg tttctttgtt gtaggtggcg gccatgtggt
     4501 gccatttgcc ttgtggggca ggcgagccca tttgtacggt ttgttcggtg ccgtaatgcg
     4561 ttttgcagat ggtttgttgc tgcgatctca gtgctcccca catggctccg ttggcctcgg
     4621 tagccagttg gtccgagttg gagaaattcc atacccagtt gccgtaagcg ctcagattgc
     4681 cgtagctttc gtccactctg aaaaagatac cgatactgta atcttccagc gcgtata
//
LOCUS       DAOSTD010000112         4676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_16655_length_4676_cov_11.273750, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000112 DAOSTD010000000
VERSION     DAOSTD010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16655_length_4676_cov_11.273750"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PLI37_06955"
     CDS             complement(<1..817)
                     /locus_tag="PLI37_06955"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOQ58782.1"
                     /translation="MASLWDNIKFRYLQGNIATKLIFINVGIFLLIKLFVVFCRLFQY
                     DGSQLILLLQLPAAWDQILLKPWTLFTYMFVHLGFFHLLFNMIWLYVFGLYFLRWFSS
                     RQLLMVYLLGGLTGGLFYVIAYNLFPLYIASVEHTVLMGASASVLALTLSVAFYRPNE
                     SLLFMFIGRVKLKWLAVAMVVIDLLSLAGDNAGGSLAHLGGALFGLLFGFFSRENGSV
                     SIFGSKIGGPLGFRAKTGRRTKYHRPRFEKANRGRPESDQEYRDRKKNEEDYRD"
     gene            complement(798..1568)
                     /locus_tag="PLI37_06960"
     CDS             complement(798..1568)
                     /locus_tag="PLI37_06960"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOQ58783.1"
                     /translation="MSVYTPRNSFWSSIPPVTKNLIIINFLLWLAATLFPTIQDFLGL
                     HYFKSDKFNLLQPVTYMFMHSTRGIGHVFFNMFAVFMFGRLLEQVWGPKRFLLFYLVT
                     GVGAGIIQQITWYIDLIPFMQAVDNYIITGEVGILGKYWNIDPSVPYSTEMVLRSRDA
                     LLDAMLTVGASGAVFGILLGFGMLFPNAPLYIMFIPVPIKAKYMVIGYGVIELFAGVV
                     NFSGDNVAHFAHLGGMIFGYFMIRFWKKKGIGHGIFMG"
     gene            1801..2070
                     /locus_tag="PLI37_06965"
     CDS             1801..2070
                     /locus_tag="PLI37_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOQ58784.1"
                     /translation="MNKTELISAIAEKAGLSKVSAKKALEAFLSSVEETLAKGEKLTL
                     VGFGTFSVVKKAARTGVNPATKQPIKIPAKKVVKFKAGANLAAKV"
     gene            2156..3949
                     /gene="argS"
                     /locus_tag="PLI37_06970"
     CDS             2156..3949
                     /gene="argS"
                     /locus_tag="PLI37_06970"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOQ58785.1"
                     /translation="MNIYTLLEDKTRQCLQALYQLQADDSLLQLQKTKKEFEGDLTLV
                     VFPFIKAAKKSPEATAEEIGSWLLQHTPELSSYNVIKGFLNLSFSASYWLNTLQDIAG
                     DTDFELFEAGESAPLVMIEYSSPNTNKPLHLGHIRNNLLGFSLSRIVAANGCRVVKTN
                     IVNDRGIHICKSMLAWQKWGKGETPESSGKKGDHLVGDYYVLFDKNYKQELAELQKQG
                     LSLEEAENTSHLMREAREMLRAWEAGDSEIRSLWETMNNWVYLGFEESYAKLGVDFDT
                     IYYESQTYLSGKEKVLEGLDKGIFYRNDDGSVWADLSSDGLDQKLLLRADGTSVYMTQ
                     DIGTAALRFEEYPIDKMIYVVGNEQNYHFKVLSILLDKLGFVWGKDLVHFSYGMVELP
                     EGKMKSREGTVVDADDLIEEMISTARETSEELGKLDSCSSEEAADIARIVGLGALKYF
                     ILKVDPRKNMTFNPKESIDFNGNTGPFIQYTYARIRSVLRKAEELGYRAQVQEQLADC
                     LADKELSLIKNLYEFPVVVKEAGNSYSPAVIANYVYELAKEYNQFYHDYSVLYETNSD
                     LRDFRILLSDQTARVIRTALYLLGIEVPERM"
     gene            3968..>4676
                     /locus_tag="PLI37_06975"
     CDS             3968..>4676
                     /locus_tag="PLI37_06975"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ58786.1"
                     /translation="MKIAFHGATQTVTGSKHLISLSNGKHILLDCGMFQGLGKETVEL
                     NNTFGFNPSEVSYLILSHAHIDHCGLIPKLVKDGFRGPIFGTPATLDLSEILLLNSAY
                     IQENDVRFLNKKRFLEGRRPLEPLYTAEHVRQALTQFQKVPYNSAFGIDDDIELQLYD
                     AGHILGASTVYLRLKENNKITRIAYSGDVGRYGETILRSPQPFPQADYIIIESTYGDR
                     LHQDADPPEDYLYEQIVE"
BASE COUNT         1420 a         1091 c         1025 g         1140 t
ORIGIN      
        1 catccctgta atcttcttcg ttttttttgc gatcccggta ttcctgatcg ctttcgggcc
       61 tgcctctgtt tgctttttca aaacgcggac gatggtattt ggtccttctg ccggttttgg
      121 cccggaagcc gaggggacct ccgattttcg aaccaaaaat acttacactc ccattttctc
      181 ttgaaaaaaa tccgaaaagc aagccgaaca aggctccgcc caaatgcgcc agactaccgc
      241 cggcattgtc tccggccagg ctcagcaaat caatcacgac catggctaca gccagccatt
      301 taagcttaac ccggcctata aacataaaca acagactttc attgggccga taaaaggcca
      361 cggataaagt caaggccagt accgaagccg aagcgcccat caacacggta tgttcgaccg
      421 aagcgatgta aagagggaaa agattgtagg cgattacata aaataaacct cccgtcaggc
      481 cacccagcaa ataaaccatc aggagctgac ggctactgaa ccaacgtaaa aagtacaggc
      541 cgaatacata cagccagatc atgttgaaca acaaatggaa aaaccccaga tgcacaaaca
      601 tgtaagtgaa aagagtccaa ggttttaaca aaatttgatc ccaggcagcg ggaagttgca
      661 acaagagtat aagctgactc ccgtcgtatt gaaacaagcg gcagaaaacc acaaacagct
      721 tgatgagcaa aaatatcccc acattgataa atatcagttt tgtggcgata ttcccctgta
      781 gatatctgaa tttaatgtta tcccataaag atgccatggc ctattccctt ttttttccag
      841 aaacggatca tgaagtatcc gaaaatcatt cctccgagat gagcaaagtg agccacgtta
      901 tccccgctaa aattgaccac tccggcgaaa agttcaatca caccgtaacc gattaccata
      961 tacttagctt tgatgggtac cggaataaac ataatgtaga gaggcgcgtt gggaaacaac
     1021 attccaaaac ccagtaatat accaaatacg gctccggaag ccccgacagt aagcatggca
     1081 tcgagaagag catctcgtga acgcaaaacc atttcggtcg aataaggtac cgaagggtct
     1141 atgttccagt atttccccag aataccgact tctccggtta ttatataatt gtctaccgcc
     1201 tgcataaaag gaatcaaatc aatataccag gtgatctgct ggatgattcc cgctcccact
     1261 ccggttacca ggtagaataa caaaaagcgc ttcgggcccc acacttgctc gagcaaacgc
     1321 ccaaacataa aaacggcaaa catgttaaag aatacgtgcc cgatacccct ggtactgtgc
     1381 ataaacatgt aggtgacagg ctgaagcaaa ttgaacttat cggacttaaa ataatgcagg
     1441 cccaaaaagt cctgaatagt aggaaagagc gtggcagcca gccagagtaa aaaatttata
     1501 ataatcagat ttttggtaac cggaggtatg gaagaccaaa aggaatttct gggagtgtat
     1561 accgacatag aaaaaatatt tgggcaaagg taaggatttt tccaaaatcc acttatcttt
     1621 gtctcgctaa acagccgtat ccatcgaaat tcgaagaaaa aagcacttcg ggagtgtttt
     1681 tttttaagaa ttacgctttt ttgtttgttt tatttgcaaa acttaaaatt ttcattttta
     1741 tttgcgttca agttttggat ccctccttat ttttcatttt tatttattat cctaattatc
     1801 atgaacaaaa cagaacttat cagtgctatt gccgaaaaag ccggcttaag caaagtgagt
     1861 gcaaaaaaag ctttggaagc atttttgagt agtgtagagg aaaccttggc aaaaggtgaa
     1921 aaactgactt tggttggttt tggtactttc tcagtggtaa aaaaagctgc ccgtaccggt
     1981 gtgaaccctg ccaccaagca acctatcaaa atccccgcca aaaaagtcgt aaaattcaaa
     2041 gccggtgcaa atttggctgc caaagtatga agctaagctt cttacgcaaa aaaaccgctt
     2101 ggatttccag gcggtttttt taattttgca acttgatttt tcttgcccga ttacaatgaa
     2161 catatacacc ctgctcgagg ataaaacccg ccaatgcctg caggctttat accaattgca
     2221 ggctgacgat tcgctgcttc agttgcaaaa aacaaaaaaa gaatttgagg gtgacctgac
     2281 cctggttgtc ttccctttta taaaggctgc caaaaaatcg ccggaagcta cggcggagga
     2341 aatcggtagt tggttactgc aacatactcc ggagttaagc agttataacg taatcaaagg
     2401 ttttctgaat ttaagtttct ctgcttcgta ctggctgaat actctgcagg atatagccgg
     2461 cgacactgat tttgagctgt tcgaagccgg cgagtcggct ccattggtga tgatagaata
     2521 ctcttcgccg aataccaaca aacctctgca tttaggacat ataagaaaca acttgctggg
     2581 attcagcctc agtcgcatcg ttgcagccaa cggctgccgg gtagtgaaga ccaacatagt
     2641 aaacgacaga gggatacata tctgtaagtc catgctggcc tggcaaaaat ggggcaaggg
     2701 cgaaacgccc gaatcaagtg ggaaaaaagg agatcatctg gtgggcgatt attatgtgtt
     2761 gttcgataaa aactataaac aagagctggc agaactccaa aaacaaggac tgagccttga
     2821 agaagccgaa aacacttcgc acctgatgcg ggaagcccgc gaaatgctgc gggcatggga
     2881 agccggcgac agcgaaatcc gcagcttgtg ggaaactatg aacaattggg tttacctggg
     2941 ttttgaagaa agttacgcca agctgggcgt ggacttcgat acaatttatt acgaatcgca
     3001 aacctatctt tcgggcaaag aaaaagtgct cgaaggcctc gacaaaggca tcttttaccg
     3061 caacgatgac ggctcggtct gggcagacct cagctcagac ggtctggatc aaaaattact
     3121 gctgcgtgcc gacggcactt cggtttacat gactcaggac ataggcacgg ctgctctgcg
     3181 cttcgaagaa taccccatcg acaaaatgat ttacgtggtg ggaaatgagc aaaattatca
     3241 cttcaaggtt ttatccatcc tgctcgacaa attgggtttt gtctggggca aagatttggt
     3301 gcatttttct tacggcatgg tggagctgcc cgaaggcaaa atgaaatcgc gcgagggtac
     3361 ggtagttgac gccgacgacc tgatagaaga aatgatttca actgcccgcg aaacttcgga
     3421 agaactgggc aagctcgatt cctgcagttc ggaggaagct gccgatattg cccgtatcgt
     3481 gggcctggga gctttaaaat actttattct caaggtcgat ccccgcaaaa acatgacctt
     3541 taatcccaaa gaatccatcg atttcaatgg gaacaccgga ccctttatcc aatacactta
     3601 cgcccgtata cgttccgttt tgagaaaagc ggaggaattg ggataccggg cacaagtaca
     3661 ggaacaactg gccgactgct tagcggacaa ggaattatcc cttatcaaga acctgtacga
     3721 gtttccggta gtagtaaaag aagccggcaa cagttatagc ccggcagtca ttgcaaatta
     3781 cgtgtatgaa ctggccaaag agtacaatca attttaccac gattattctg ttttgtacga
     3841 gaccaactcc gatttgagag attttcgtat ccttctttcg gatcaaacag ccagagtaat
     3901 cagaacggcc ctgtatcttc tgggcatcga agtacccgag cggatgtaat cgtaaaaccg
     3961 gaatactatg aaaatcgctt ttcacggggc aacccaaact gttacgggct ccaagcactt
     4021 aatatcactt tcgaacggta aacatattct gttggattgc ggcatgtttc agggtttggg
     4081 caaagaaaca gtggaactaa acaacacatt cggtttcaat ccttcggaag tcagttacct
     4141 gattctttcg cacgcgcaca tagatcattg cggactgatt cccaagctgg tcaaggatgg
     4201 ctttagggga cccatcttcg gaacccctgc cactctggat ctttccgaaa ttctattgct
     4261 caattccgcc tatatacaag aaaacgacgt tcgctttttg aacaaaaaac gttttctcga
     4321 aggccgccgg cctttagagc ccctttacac cgcagagcat gtcagacaag ccctgactca
     4381 gtttcaaaaa gtaccctaca acagcgcttt tggaatagat gacgatatag aacttcagct
     4441 ttacgatgcc ggacatatcc tgggagcctc cacggtttat ctgagattaa aggaaaacaa
     4501 caaaatcaca cgcattgcct acagcggaga cgtaggccgc tacggcgaaa ccatactccg
     4561 atctccccag ccttttccac aggccgatta catcattata gaatccacct acggcgaccg
     4621 tctgcatcag gatgccgatc cgcccgaaga ttatctctac gaacaaatcg tggaaa
//
LOCUS       DAOSTD010000113         4523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_17369_length_4523_cov_14.074306, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000113 DAOSTD010000000
VERSION     DAOSTD010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17369_length_4523_cov_14.074306"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PLI37_06980"
     CDS             <1..686
                     /locus_tag="PLI37_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOQ58787.1"
                     /translation="RMAALMEVPVKFIWTHDAFRVGEDGPTHEPVEQEAQIRLLEKLK
                     NHKGNNSMLVLRPADANECTVAYKMALENTQTPTAFILSRQNIKNLPGSSYKQALQAE
                     KGAYVVECDGDFDVILLASGSEVSTLVEGAELLRKDGIKLRIVSVPSEGLFRNQSQAY
                     QEEVLPAGSKIFGMTAGLPVTLQGLVGDKGKIWGLESFGFSAPYKVLDEKLGFNAANV
                     YKQVKSFLA"
     gene            782..1714
                     /locus_tag="PLI37_06985"
     CDS             782..1714
                     /locus_tag="PLI37_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOQ58788.1"
                     /translation="MNRRNFLKIFALTGVGLAVKPAKSLAALVPEENKTEQAVDLVAV
                     MGGEPDQMFKRAIAEFGGMNQFVKSGQKVCIKPNIGWDRVPERAGNTNPLLISEIIRQ
                     CFAAGASEVTVFDHTCDDWVKCYQNSGIEAAAKEAGAKVLPGHQESYYRQVELPKGKV
                     LKDAKIHQAILDSDVWFNVPILKNHGGSGLTISMKNYMGIVWDRRAFHMKGLQQCIAD
                     VCTLDKKPALNIVDAYRVLQTNGPQGRSESDVVLSKALFVSPEIVSVDTAATSFFNQI
                     RSMPMDVVKHLGFGEEHGLGTTDLTKLNIKRIRL"
     gene            1725..3368
                     /locus_tag="PLI37_06990"
     CDS             1725..3368
                     /locus_tag="PLI37_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOQ58789.1"
                     /translation="MLRKLRIALALVAFAVLTFFFLDFSRLLPESVQVIAKIQFVPAL
                     ISLSLGIILLLLLLTLVFGRIYCSVICPLGIYQDVISRLANLFRKKKLRYKFRKAKTI
                     LRWLVLTLVIITYLSGLGLVLDLLDPYSMYGRMAVHLFKPVYMAANNGLELLFTAFNN
                     YTFYKVDIHFGGWASFGLALISLLVVSVLAWRFGRTWCNTICPVGTVLGFLSKLSVFK
                     LSIDHETCNQCGLCSFNCKATCIDSKKGSIDYSRCVACFNCMESCNKKGVKYLPFWKV
                     KKTGTDKTQVKTVSGKIQAKTKTKTKTKKRIETDTDTKIEVKTINKTKTDASKREFLS
                     VLMMSSLLMPKALAAGFRQQISSLRPLKRTVPMMPPGAGDRARFNSLCTSCHLCVSKC
                     PGDVLQPSLLEYGVEGIMQPQMLFDKGFCNYDCTLCTEVCPSGALKTLTKEEKHRTQT
                     GRVVFVLENCIVYRNETHCGACSEHCPTQALRMVPYKGHLTIPEPHPDICIGCGGCEF
                     ICPVRPYKAVFVEGYEIQQVAKAFEDEEKQDIVLDDFGF"
     gene            3386..3592
                     /gene="tatA"
                     /locus_tag="PLI37_06995"
     CDS             3386..3592
                     /gene="tatA"
                     /locus_tag="PLI37_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOQ58790.1"
                     /translation="MYYLPLLLGLGTSELILIALVILLLFGATRIPEIMKGIGKGIKS
                     FKKEINSTDDEEPLNNKKEKDSDK"
     gene            3605..4390
                     /gene="tatC"
                     /locus_tag="PLI37_07000"
     CDS             3605..4390
                     /gene="tatC"
                     /locus_tag="PLI37_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOQ58791.1"
                     /translation="MTEEKLTFWEHLDELRVVLIRIVIVLVAVTVIAFAFKEPLFKII
                     LAPHKADFILYRLINRLSSLLAWPQMSLPEFEVKLISTELTAQFFTHMKVAFFAALLV
                     SSPYIVFILFRYISPALYHNEKKYSGRVLFWAYSLFFLGVLLSYYIIFPLSFRFLGTY
                     QVSPEVVNTITLNSYVQTLLVLSLLMGIMFEIPVLAWFFAKIGLLKADFMRKKRRYAI
                     VIILIVAAVITPTGDVFTLTIVSLPVFLLYEISILLVAKTSGA"
BASE COUNT         1254 a          911 c         1012 g         1346 t
ORIGIN      
        1 ttcgtatggc ggctttgatg gaagttcccg ttaaattcat ctggactcac gatgcattca
       61 gagtaggtga ggacgggccc acgcatgagc ctgttgaaca agaagctcaa atccgtctgc
      121 tggaaaaact caaaaatcac aagggcaata attccatgtt ggttttgcgt ccggcagatg
      181 ccaatgagtg taccgttgca tacaaaatgg ccctggaaaa tacgcaaact cctacagcct
      241 ttattttgag ccgtcagaat attaaaaatt tacccggtag ttcgtacaaa caagccctac
      301 aagctgaaaa gggtgcttat gtggtcgaat gtgacggtga ttttgatgtt attctgctgg
      361 cctcgggttc ggaagtttct acactggtcg aaggtgcaga attattgcgt aaagacggta
      421 ttaaactgcg tatagtatct gttccttccg aaggtttgtt ccgtaaccaa agccaggcct
      481 atcaggaaga ggttttacct gccggttcca aaatctttgg tatgacggcc ggtttgccgg
      541 ttactcttca gggactcgtt ggcgacaaag ggaagatctg gggattggaa tcgttcggtt
      601 tttcggctcc ctacaaagtt ctggacgaaa agctgggctt caacgctgcc aatgtttata
      661 aacaggtaaa atcctttttg gcttaattaa taattgttta taacctattt ttttcgggaa
      721 actatggtat atttgccagc gtttcccgaa attttatttt aaaaactaca actatttggc
      781 catgaacaga agaaattttt tgaagatttt cgctttaacg ggagtaggac tggcagtaaa
      841 accggccaag tcgctggcag ctcttgttcc cgaagaaaat aagaccgaac aggctgttga
      901 tttggttgct gttatgggcg gagaacccga ccagatgttc aaaagggcta ttgccgaatt
      961 cggaggcatg aatcaatttg tcaaaagcgg acaaaaggta tgcatcaaac caaatattgg
     1021 ttgggataga gtacccgaaa gagccggtaa taccaatcct ctacttataa gcgaaatcat
     1081 aaggcagtgt ttcgccgccg gagcctcaga ggtaacggta tttgaccata cctgcgacga
     1141 ttgggtcaaa tgttatcaaa acagcggaat cgaagccgcc gccaaagaag ccggagccaa
     1201 agttctgccc ggccatcagg aatcttatta tcgtcaggtg gaattaccta agggtaaggt
     1261 acttaaagat gccaagattc atcaggctat actggacagt gatgtctggt tcaatgtacc
     1321 tatactcaaa aatcatggag gctccggatt aaccatctct atgaaaaatt atatgggcat
     1381 agtctgggac cgaagggcct ttcatatgaa gggcttgcaa caatgcattg ccgacgtttg
     1441 taccctggat aaaaaaccgg cacttaatat agtagatgcc tatcgtgttt tacaaactaa
     1501 cggaccacag gggcgttccg agtcggacgt tgttttatca aaagccttgt ttgtttcccc
     1561 ggaaattgtg tcggtcgata ctgcggccac ttcttttttc aatcaaatcc gttccatgcc
     1621 tatggatgtg gtcaaacatc tcggattcgg cgaagaacat ggattgggaa ctactgattt
     1681 gaccaaattg aacattaaaa ggatcaggct ataattgaat cataatgctc agaaagctta
     1741 gaatagctct ggccctggtg gcctttgcag tactaacatt tttttttctg gatttttccc
     1801 gtcttctgcc ggaaagtgta caagttatag ctaaaattca gtttgttccc gctttgattt
     1861 cgctttcact gggaattatt ttactgctgt tgctgctcac tttggttttt ggcagaatct
     1921 attgttctgt tatttgtccc ttgggcattt atcaagatgt gatttccagg ctggccaacc
     1981 tttttcgcaa gaaaaaactc cggtataaat tccgcaaggc aaaaacgatc ttacgctggt
     2041 tggttttaac cttggtgatt atcacctacc taagcggcct tggattggta ctggatttgc
     2101 tggatcctta cagtatgtac ggaagaatgg ccgttcacct attcaagcct gtatatatgg
     2161 ctgctaacaa tggcctggag cttttattta ctgccttcaa caattacact ttttataaag
     2221 tcgatattca ttttgggggt tgggcttctt ttggactcgc ccttataagc ttattagtgg
     2281 tctccgtttt ggcctggcgt tttggtcgca cttggtgcaa taccatttgt ccggtgggta
     2341 ccgttttagg ctttttgagc aagttatctg tcttcaaatt gagtattgat cacgaaacct
     2401 gtaaccaatg cgggctttgc agtttcaatt gtaaggccac ctgtatagac agtaaaaagg
     2461 gaagcatcga ttacagccgt tgcgtcgctt gtttcaattg catggagagt tgtaacaaaa
     2521 aaggcgtaaa gtatctgcct ttttggaaag taaaaaaaac agggaccgac aaaactcagg
     2581 taaaaacagt aagcggcaaa attcaggcaa aaaccaaaac aaaaaccaaa acaaaaaaaa
     2641 gaattgaaac cgatactgat acaaagattg aggtaaaaac aataaacaag acaaaaacgg
     2701 atgcttccaa aagagaattt ttatcggtct tgatgatgag cagtttattg atgcccaaag
     2761 ctttggctgc cggcttccgt caacagatca gcagcctacg ccctctaaag agaactgttc
     2821 ctatgatgcc tccgggcgca ggagacagag ctcgtttcaa tagtttgtgc acttcctgtc
     2881 atctctgtgt cagcaaatgc ccgggcgatg tgctgcaacc cagcttgctg gaatacggtg
     2941 tcgaaggtat tatgcaacct caaatgttgt ttgacaaggg cttttgcaac tacgactgca
     3001 ccttgtgtac cgaggtgtgc cccagcggag ccttgaaaac tctgactaaa gaagagaagc
     3061 atcgtacaca aacgggaagg gttgtttttg tgcttgagaa ctgcattgtt tatcgtaatg
     3121 aaacccattg cggagcctgt tcggaacatt gtcccaccca ggcgcttaga atggtgccct
     3181 ataaagggca tctgaccatt ccggaaccgc atcccgatat ttgcataggt tgcggaggtt
     3241 gcgaatttat ttgccccgta aggccctata aggctgtttt tgtagaagga tatgagattc
     3301 aacaggttgc caaagctttc gaagacgaag aaaagcaaga tattgtcctg gatgattttg
     3361 gattttaaca ttaataataa tgattatgta ttacctgcct ttattacttg gtttaggtac
     3421 ctcagaacta attcttattg cactggtaat cctcttattg tttggagcca ccagaattcc
     3481 tgaaatcatg aaaggaatag gcaagggtat caagagtttc aaaaaagaga taaatagtac
     3541 cgacgacgaa gaaccgctta acaacaaaaa agaaaaagac agcgataaat aataaaaaag
     3601 ctgcatgacg gaggagaagc ttacattctg ggaacattta gacgaactca gagttgtttt
     3661 aattcgcata gttatagttt tggtagctgt gactgtgatt gcgtttgcct tcaaagagcc
     3721 cctttttaaa ataatcttgg cgccacacaa ggccgatttc atactctaca ggcttatcaa
     3781 tcgattgtct tccttgcttg cctggcctca gatgtccctg cccgaattcg aagttaaatt
     3841 aattagcacg gagcttaccg cccagttttt tacgcatatg aaagttgctt tttttgcagc
     3901 tctactggtg agttctccgt acatagtatt tattctcttt cgttatattt cgccggctct
     3961 ttaccacaat gaaaaaaaat attccggacg tgtactcttc tgggcctata gtttgttttt
     4021 tcttggtgtg ttgcttagct actatattat tttcccgctc tcttttcgtt ttttgggcac
     4081 ttatcaggtt agtccggaag tggttaatac cattaccctg aattcgtatg tgcaaactct
     4141 gttggtatta tcactcttga tgggtataat gtttgagata ccggtattgg cctggttctt
     4201 tgctaaaatc ggtctgctta aagctgattt tatgagaaag aaacgcaggt acgccattgt
     4261 tataattctg attgttgccg ccgtcattac tccaaccggc gatgtcttta ctcttacgat
     4321 tgtctcttta cccgttttcc tgctttacga aatcagcatc ttgctggtgg caaaaacatc
     4381 cggggcttaa atactatctt tgcgcgataa ggtgtttttg agcttgataa aataaatgta
     4441 ggtaatggga acaatgctga taatattcga aattatcagg ggtttgtctc taatgacaat
     4501 tccataaatc agccataaag taa
//
LOCUS       DAOSTD010000114         4472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_17621_length_4472_cov_15.375368, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000114 DAOSTD010000000
VERSION     DAOSTD010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17621_length_4472_cov_15.375368"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1022
                     /gene="lgt"
                     /locus_tag="PLI37_07005"
     CDS             117..1022
                     /gene="lgt"
                     /locus_tag="PLI37_07005"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOQ58792.1"
                     /translation="MGVLDVVTWTFPPQLFNLFGKEVRWYGLLFALGFYFGLVITSKM
                     FKAEKVPDKWVDKLFIYIIIAAAVGARLGHVFFYAWDYYSQHPWEIIKIWEGGLASHG
                     GAIGIILAVWLYSKRVTKRSLNWTFDRIVVSVALAGALIRLGNLANHEIYGHPTDLPW
                     GFRFITNWYQVRRGADPVFSEPSHPTQIYEALCYLLCFVVLYNLYWKTKLKHREGFLF
                     GVFLIGVFLSRFLIEFVKENQETFEEGMLLNMGQLLSIPFVIAGVWLIIRAFRRPPVY
                     YETNEKPKKDKTNTLLSNVKSTSVK"
     gene            1019..1999
                     /locus_tag="PLI37_07010"
     CDS             1019..1999
                     /locus_tag="PLI37_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58793.1"
                     /translation="MSWQKVLLFVATLLFLSACSSEARYRVPGKPKHPLVFTRYEQDF
                     FEDGRIEDSVFRDLFSNRIMQFGSVGEARTDSFLELFRLDSTMQKVYTDCQALFPDLE
                     IYQKQLSVAFHRLCHFVPDMPLPSVSTHLSGFSQSVVSAPSILSVSLDKYLGSDYVLY
                     QSLFYDYQRQRMRAEQLVPDCLNGWLRSEFTNASIMADSRLLDFIIYEGKILFVLKLL
                     FPKTEIEYLTGWTKEQADWCAVNEKRMWERILEYEHLYSRDHLVLSKYIGDGPATVYF
                     TQESPSRAVLWTGLKIVEAYMQRHKKLSVLDMMMETDADKILTESLYRPK"
     gene            complement(2030..2947)
                     /gene="metA"
                     /locus_tag="PLI37_07015"
     CDS             complement(2030..2947)
                     /gene="metA"
                     /locus_tag="PLI37_07015"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOQ58794.1"
                     /translation="MPLNLPPQLPAIDILKKENIFVMDENRASTQDIRPLRIVVLNLM
                     PIKITTETDLIRLLSNSPLQLEMKLMKIKSHTPKNTPVEHMKAFYETFERLRSEKFDG
                     MIITGAPVEHLDYEEVTYWEEVKEIFDWSRTHVTSTMYICWAAQAGLYHQYGIPKYNL
                     PEKKFGIFKHRANQPEYPLFRGFDDEFYVPHSRYTEIRREDILNKPELTLLAESEEAG
                     VYIVMGRNGREFYLTGHSEYAPDTLHKEYLRDLEKGLKIAVPSNYYTDDDPTKQPLVR
                     WRGHANLLFSNWLNYFVYQETPFDISQVH"
     gene            3043..>4472
                     /locus_tag="PLI37_07020"
     CDS             3043..>4472
                     /locus_tag="PLI37_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOQ58795.1"
                     /translation="MNTSRFKLNKVFVPKFYELIKSKQYSKALFVKDLTAGVIVGVIA
                     IPLAIAFGIASGVSPQQGLITAIIGGLVISLFGGSHVQIGGPTGAFIVIVAGIVAQFG
                     LTGLIIATAIAGVLLVLMGLLKLGDAIKFMPYPIIVGFTSGIALVIFSTQIKDFFGLK
                     ALDGSALSVPSDFIEKWICYAKHLGTINPWATGIAVFTVVLIFLWKKVSKVIPGSLVA
                     IVVSTLLVVVLKKYGVEIDTIGNVYPELSGGTPLPKPSTPPITLQSIRLLLGPAFTIA
                     MLGAIESLLSAMVADGVTGKKHHSNTELIAQGMANITSSFFGGIPATGAIARTMANIN
                     NGGRTPVAGIVHAIFLLLVYLFLMPLAANIPFACLAGILVVVAYNMSEWRSFKSLLKN
                     PKSDVLVLLLTFFLTVIFDLTIAISLGLLLACILFIKRVMETSSIKVIENEIERTEDP
                     EAIAILEKISDDIEIYEIDGPFFFGVA"
BASE COUNT         1145 a          995 c         1009 g         1323 t
ORIGIN      
        1 gctaaacgct acttttcggg ctgcgcccgg gaagatagct acgctcggca atagcaaaat
       61 aaattttgct attgctctcg ctaaacgcta tctttgagca ctcaaaaaaa agaaaaatgg
      121 gagttttgga tgttgttacc tggacttttc cgcctcagct tttcaatttg tttggtaagg
      181 aggtacgctg gtacggttta ttgtttgctt tgggctttta ttttgggttg gtaataacct
      241 ccaaaatgtt caaagccgag aaagtgcccg ataaatgggt agataaactc tttatttaca
      301 ttattattgc cgctgccgta ggtgctcgtc tagggcatgt tttcttttac gcctgggatt
      361 attattcgca gcatccctgg gaaatcatta agatttggga aggcggactg gccagccacg
      421 gcggtgcgat tggtatcatt ctggcggttt ggctctacag caaaagagtc actaagcgca
      481 gtctcaactg gactttcgac cgtattgtag tatcggtggc tttggcaggc gccctcataa
      541 gactggggaa tctggcaaat cacgaaattt acggtcatcc caccgattta ccctggggct
      601 tccgctttat aaccaattgg taccaggtgc gtcgcggagc cgatccggtt ttttcggagc
      661 cctctcaccc cacccagatt tacgaagctc tttgctacct gctctgtttt gttgttttat
      721 acaaccttta ttggaaaaca aaactcaaac accgagaggg ttttttgttc ggcgtatttt
      781 taattggagt attcctgtcc cgatttttga ttgaatttgt caaagaaaat caggaaactt
      841 tcgaagaagg catgctgttg aatatggggc aactcctgag tattcccttt gtgattgccg
      901 gcgtctggct catcatcagg gcatttcgtc gtcctcccgt ttattatgaa acaaacgaaa
      961 aacctaaaaa ggacaaaacg aatactcttt tgagtaatgt aaaatcaact tcggtaaaat
     1021 gagctggcaa aaagtcttgc tctttgttgc tactctcttg ttcttgagtg cctgcagcag
     1081 cgaagcgcgc tatcgcgtgc ccgggaaacc caaacatcct ttggtattta cacgttacga
     1141 gcaagacttt ttcgaggacg ggcgcatcga agactctgtt tttagagatc ttttctctaa
     1201 ccgaatcatg caattcggtt ctgtaggtga ggcccgtacc gattccttcc tcgagttgtt
     1261 tcgcctcgac tccaccatgc aaaaagtcta caccgattgc caggctttgt tcccggacct
     1321 cgaaatatac caaaaacaac tctcagtggc ctttcacagg ctgtgtcact ttgttccgga
     1381 catgcctctg ccgtctgtat ccacccattt atcgggtttt agtcaatcgg tggtttcggc
     1441 tccgtccatc ctttcggtca gtctcgacaa gtatctgggc agtgactatg ttttgtacca
     1501 gagtctgttt tacgattacc aacgccaacg catgagagcg gaacaactgg ttcccgattg
     1561 tctgaacggc tggctacggt ctgaatttac caatgcctct atcatggccg acagccgttt
     1621 actggatttc attatttacg agggcaaaat actgtttgtt ctcaaattgc tctttcctaa
     1681 aacggaaatt gagtacctaa caggctggac caaagaacag gcagactggt gtgccgtcaa
     1741 cgagaaaaga atgtgggagc gcatattgga atacgagcat ctgtacagca gagatcacct
     1801 ggtcttgagt aaatacatag gagacggacc cgctacggta tattttaccc aggaatcacc
     1861 atcaagagcc gtactctgga cagggctgaa aattgtagaa gcctatatgc agcgacacaa
     1921 aaagctcagc gttttggata tgatgatgga aacggatgca gacaaaatcc tgaccgaatc
     1981 gctttacaga ccgaagtaac aaaaactcgt gctttcctgc cggcagtcat caatgcacct
     2041 ggctgatatc aaagggagtt tcctgatata caaaatagtt caaccaattg ctaaacaata
     2101 ggttagcatg ccctctccaa cgaaccagag gttgtttggt gggatcgtca tcggtgtaat
     2161 aattacttgg gacagctatt ttcaggcctt tttccagatc tcggaggtat tctttgtgca
     2221 aagtatcggg cgcgtactcc gaatggccgg ttagatagaa ttccctgccg ttgcgaccca
     2281 taacaatata cactccggct tcttcggatt cggccaacag agtcaattcc ggtttgttta
     2341 aaatatcttc acggcgaatt tcggtataac ggctgtgagg cacgtaaaat tcatcgtcaa
     2401 aacctctaaa caaaggatat tccggttgat tggcccgatg tttgaaaatg ccgaattttt
     2461 tttcgggtaa attgtatttg ggaattccgt attgatggta caaaccggct tgcgcagccc
     2521 aacagatata catagtcgag gtaacgtgag tgcgcgacca atcgaatatt tctttgacct
     2581 cttcccaata agtgacctcc tcataatcaa gatgctcaac cggggctccg gtaataatca
     2641 tcccgtcaaa tttttcgctg cgcagtcgct caaaggtttc gtaaaaagcc ttcatatgct
     2701 ccaccggagt gttttttggc gtatgacttt ttattttcat taacttcatt tccaactgca
     2761 aaggagaatt ggaaagtaga cgaatcaagt cggtttcggt tgtgattttt ataggcatta
     2821 aattaagcac cactatcctg agtggacgaa tatcctgggt agatgcccgg ttttcgtcca
     2881 ttacgaagat attttccttt ttcaagatat ctatagcggg taattgagga ggcagattta
     2941 atggcatatt cttaaaatga gaaatttagc ccacaaaagt acggcaaaat ttcgtctaaa
     3001 tggctatctt cgcacaattt ttagtctggg tggatagaaa aaatgaatac tagtagattt
     3061 aagctcaata aggtatttgt accaaagttt tacgaactga ttaaaagcaa gcaatactcc
     3121 aaggccttgt ttgtaaaaga ccttacggcc ggtgttattg tgggagtgat tgccatacct
     3181 ttggccattg cttttggtat tgccagcgga gtgagtccgc aacaaggcct tattactgcc
     3241 attatagggg gactggttat ctctttgttt ggtggttccc atgtgcagat tggcggtcct
     3301 accggtgctt ttattgtaat tgttgccgga atagttgctc aattcgggct tacaggattg
     3361 attatagcta ccgccatcgc cggagttttg ttggtattga tgggactgct caaattggga
     3421 gacgctatca aatttatgcc ttatcccatc atagtgggtt ttaccagcgg aatagccctg
     3481 gttatttttt caactcagat taaagatttt tttggtttga aagctttgga cggctcagct
     3541 ttatctgtgc cctccgactt tatcgaaaaa tggatctgtt acgccaaaca ccttggaaca
     3601 atcaatccct gggctaccgg tattgctgta tttaccgtcg ttctgatatt tctttggaaa
     3661 aaagtcagta aagtcattcc gggatcactg gtagctatcg ttgtttcgac cttgctggtt
     3721 gttgtactta aaaaatacgg agtcgaaatc gacaccatcg gaaatgttta tcctgaactg
     3781 agcggtggca ctccactacc caaacccagc acgccaccca ttaccctgca atccatccgg
     3841 ttattacttg ggccggcatt taccatagcc atgttgggcg ctatcgaaag tctgctctct
     3901 gccatggtgg cggacggtgt taccggtaaa aaacaccatt ccaataccga gttgatcgct
     3961 cagggcatgg caaatatcac gtcctccttt tttggcggca ttcctgccac aggagccatt
     4021 gccagaacca tggccaacat taacaacgga ggccgcactc cggtggccgg catcgtacac
     4081 gccatctttt tactcctggt atatctgttt ttgatgcctc tggctgcgaa tattcccttt
     4141 gcttgcctgg ccgggatttt ggtggtggtg gcttataata tgagtgaatg gcgctctttt
     4201 aaatctctgc ttaaaaaccc aaaaagcgat gtgctggtat tgttgttaac cttcttttta
     4261 acggtaattt tcgatcttac catagccatt tctctgggat tattactggc ctgcatactg
     4321 tttatcaaac gggtaatgga aacttcaagc atcaaggtaa tcgaaaacga aatcgaaaga
     4381 accgaagacc ccgaagccat cgccatattg gaaaagatca gcgacgatat tgaaatttac
     4441 gaaattgacg ggcccttctt tttcggtgtg gc
//
LOCUS       DAOSTD010000115         4442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_17766_length_4442_cov_15.284933, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000115 DAOSTD010000000
VERSION     DAOSTD010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17766_length_4442_cov_15.284933"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PLI37_07025"
     CDS             complement(<1..788)
                     /locus_tag="PLI37_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ58796.1"
                     /translation="MNFFSIDTGFFSTEAGAMFGIMSQKVWSKKYPADRENRSPLSMR
                     CLFVDFGERKVLFDTGVGNKQLRGMAYYRFRELIDIRDALKSKGYDAMQVTDVVLSHL
                     HFDHCGGCTREGDNGGFELCFPGARHWASAAQWKNSLQPSSWEADAYLPENMQAVKDA
                     GKLHLIDKDQVLFPGLELKLYQGHTYDQIVSFIRTERGNLIFSGDVVPMATHIAQGCI
                     AAVDNHALLAVEEKMRLLEEACELQAGLVFYHDFYTKIAGLKKS"
     gene            816..1709
                     /gene="rfbD"
                     /locus_tag="PLI37_07030"
     CDS             816..1709
                     /gene="rfbD"
                     /locus_tag="PLI37_07030"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOQ58797.1"
                     /translation="MKIMTRKVVLITGANGQLGSEIRLLENQYPQFAFTFTDIQELDL
                     QNSQAVFDCFSRLKPDYLINCAAYTAVDKAESDIELCYSINSAAVRNLALAAKQQNTI
                     VLHVSTDYVFDGKQARPYRENDAVCPVSVYGKSKLQGEQVLNEICPSSSVIVRTAWLY
                     SAFGNNFVKTMIRLGWEKSELRVVNDQWGSPTYGADLAQALMTIVLSAEDGHFTPGIY
                     HFSNEGKCSWYEFAQKIHQLAGITNCKIIPVTTAEYPTAAQRPSFSLLDKSKIRRDYG
                     VAVPLWTDSLKTCLSKFNNCI"
     gene            1719..2948
                     /locus_tag="PLI37_07035"
     CDS             1719..2948
                     /locus_tag="PLI37_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOQ58798.1"
                     /translation="MKHVQLKKQGGAIWFLVVSTLLFAACGQKTTKPLSVRMAESEMI
                     RTPVPTDLDGCEGRLKWNYTHGLELLAFMDLYDASGDERFWNYAARYADTMINSQAEI
                     VSYKKTNYNIDHICPGRILFRMYDRTGEAKYKMAMDTLFSQLQTHPRTSDGGFWHKKV
                     YPYQMWLDGLYMGPPFYAEYISRYVDASNQQDLFADVVHQFVTVGKHTYDTVTGVYRH
                     GWDESRAMFWADKETGQSAHCWGRGLGWYVMGLVDALDYIPGQGQRQALIDILKGIYE
                     VLPKYADPQTGMWYQVLEFPEREGNYQESTASIMFVYGLLKGVKTGVFDASYRETALQ
                     WYEKFVDRFVKENPDGTISITDCCAGAGLGGSQNRSGKYDYYINETIIIENDCKAVGP
                     FIQASLIYEEFKSERQQ"
     gene            3072..4010
                     /gene="mdh"
                     /locus_tag="PLI37_07040"
     CDS             3072..4010
                     /gene="mdh"
                     /locus_tag="PLI37_07040"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOQ58799.1"
                     /translation="MSKVSVIGVGNVGATYASIMTINEVADEIVLLDVKEGIAEGKAL
                     DMKQASCLMSIDTRISGTTGDYSKTAGSDVVVITSGIPRKPGMTREELIGVNAGIVKD
                     VVSNVLKYSPDAILVIISNPMDTMTYLVQKTSGLPKNRIIGMGGVLDSSRFKTYLSMA
                     LGDVNLNDIQACVIGGHGDTTMIPLTRYATYKGIPVSEFLSEETLNKVAADTMVGGAT
                     LTKLLGTSAWYAPGAAGAYLVESILHDQKKVLPCSVYLEGEYGQKDICIGVPVVIGKN
                     GWEKVVDFKLNAAEQALFDKSAEAVRSTNRVLDSVL"
     gene            4194..>4442
                     /locus_tag="PLI37_07045"
     CDS             4194..>4442
                     /locus_tag="PLI37_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58800.1"
                     /translation="MLKTSDICRKMAFSLVVLFCCGTAQSLCAQRDSIDQSGERFIQL
                     LQTSEKNKGTVRIFQDRRIVDLVGSKQMSANIIKDGDKT"
BASE COUNT         1215 a          956 c         1082 g         1189 t
ORIGIN      
        1 ttgctttttt tgagaccggc aattttggtg tagaagtcgt ggtaaaagac cagtccggcc
       61 tgtaactcgc aggcttcttc caaaagccgc attttttctt ccacggccag taaggcgtga
      121 ttgtccacgg ctgcaataca gccctgtgca atatgggtag ccattgggac cacatcgccg
      181 gagaaaataa gattaccacg ttccgtgcgg ataaaggaga ctatctgatc gtaggtatga
      241 ccttgataga gtttaagttc cagtcccgga aacaagacct ggtctttgtc gataagatgt
      301 aacttgccgg catctttcac ggcctgcatg ttttcgggca gataggcatc ggcttcccaa
      361 ctggaaggtt gcagggaatt tttccactga gctgcgcttg cccagtggcg ggccccggga
      421 aagcaaagtt caaatcctcc attgtcccct tcccttgtgc agcctccgca atggtcaaag
      481 tgcagatgac tcagtaccac atcggtgact tgcatggcat cgtagccttt gctttttagg
      541 gcatctctga tgtcgatcag ttcgcgaaag cggtaataag ccatacctct gagttgtttg
      601 tttcccaccc cggtatcaaa gagcactttt cgttcgccaa aatcaacaaa aaggcaacgc
      661 atactcaaag gactgcgatt ttcccggtcg gccgggtatt tttttgacca gactttttgg
      721 gacatgattc cgaacatggc tcctgcctcg gtcgaaaaaa agcccgtgtc tatgctgaag
      781 aagttcatat atttacctaa ttttgcgaca aagttataaa aattatgacg cggaaagtag
      841 ttcttattac cggagccaac ggccaattgg gtagtgaaat aagattgttg gaaaatcaat
      901 atcctcaatt tgcgttcact tttaccgaca ttcaggagtt ggatttgcaa aactcccaag
      961 cagtttttga ctgcttcagc cggttgaagc cggattattt aatcaattgt gccgcttata
     1021 cggctgtaga taaggcagaa agcgacatag agctgtgtta ttccataaac agtgctgcag
     1081 tcagaaacct tgccttagct gctaagcaac agaatactat tgttttgcat gtttccacgg
     1141 actatgtttt tgatgggaaa caagccaggc cttatcgcga aaacgatgcg gtttgtcctg
     1201 tttcggtcta tggcaagagc aaattacagg gggagcaggt gttgaatgaa atctgtcctt
     1261 cttcttcggt gatagttcgt accgcctggc tttattctgc gtttggaaac aattttgtaa
     1321 agaccatgat tcgtttggga tgggaaaaat cggaactcag agtggtgaat gatcaatggg
     1381 gcagccctac ctatggggcc gatctggcgc aggctttgat gacaatcgta ttaagcgccg
     1441 aagacgggca ttttaccccc ggcatctacc atttcagcaa cgaaggaaaa tgttcctggt
     1501 acgaatttgc tcaaaagatt catcaactgg ccggaattac aaattgtaaa ataataccgg
     1561 tgactactgc cgaatatcct acggcggccc aaaggccctc cttcagcctg ctggacaaaa
     1621 gcaaaatccg cagggattac ggagtggctg ttcccttgtg gacagacagc ctcaagactt
     1681 gtttgtcgaa atttaataac tgtatttaaa tctgtacaat gaaacatgtt caattaaaaa
     1741 aacaaggcgg agcgatatgg tttcttgttg taagcacgct tttatttgcc gcctgtggac
     1801 aaaaaaccac taaacctctt tcggttcgca tggccgaaag cgagatgatc aggacgcccg
     1861 taccaaccga tttggacgga tgcgagggtc ggctcaaatg gaattatacg cacggattgg
     1921 agttgctggc ttttatggat ttatacgatg cgagcggaga tgagcgtttc tggaattatg
     1981 ccgcccgtta tgccgatacc atgatcaact cgcaggccga gattgtttcc tataaaaaga
     2041 ccaactacaa catcgaccat atctgtcccg ggcggatatt gttccgcatg tacgacagaa
     2101 ccggagaagc taagtataaa atggctatgg acaccttgtt ttcgcagttg caaacacacc
     2161 cacgcacctc cgacggaggt ttctggcata aaaaagttta tccttatcaa atgtggctgg
     2221 acggcttgta tatggggccg cctttttatg cggaatacat cagccgctat gtcgatgctt
     2281 caaatcagca agatttattt gcggacgtag ttcatcagtt tgtcacagtg ggcaaacaca
     2341 cctacgatac tgttaccgga gtgtacaggc atggctggga cgaaagccgc gctatgtttt
     2401 gggcagataa ggaaacagga caatcggccc attgctgggg cagaggtttg ggctggtatg
     2461 taatgggcct ggtggatgct ttggattaca taccgggaca aggacagcgt caggctttga
     2521 tcgatattct caaggggata tacgaggtgc tgccaaaata cgcagatccc caaaccggca
     2581 tgtggtatca ggttttggag tttcccgaaa gggagggtaa ttatcaggaa tctaccgcat
     2641 ccatcatgtt tgtgtatggc ctgctcaaag gggttaaaac aggagttttt gatgcctctt
     2701 accgtgaaac ggctctgcaa tggtacgaga agttcgtgga tcgttttgta aaagagaatc
     2761 ccgacgggac catcagcatt accgactgtt gtgcgggagc aggattgggc ggttcacaaa
     2821 atcgcagtgg aaaatacgac tattacatca acgaaaccat tatcatcgaa aacgactgca
     2881 aggcggtagg cccgtttatt caggcttcgt tgatatacga ggaattcaag tccgaaagac
     2941 agcaataaag ttcagagcat ccaaaaagtt gcgacttaga gctttgaaag caaaaacaaa
     3001 ttgtatcttt gccaatcctt ttataaggac agtttttaat aggataataa gttaatttag
     3061 atctaaattt tatgtcaaaa gtaagtgtaa taggagtagg aaatgtaggt gcaacttatg
     3121 cgagcataat gaccatcaac gaagtagcag atgaaattgt cctcttggac gttaaagaag
     3181 ggattgcaga gggcaaagct ctcgacatga aacaagcatc ctgtttgatg agtatagaca
     3241 ccagaatttc aggtactaca ggagattatt ctaaaacggc cggttctgat gtggtagtga
     3301 ttacttcggg tattccccgt aagcccggaa tgaccagaga agaacttatt ggtgttaatg
     3361 ccggtatagt gaaagacgtt gtatccaatg tcttgaaata ttctcccgat gccatactgg
     3421 ttatcatcag taatcccatg gacactatga cttacctggt ccagaaaaca tcgggccttc
     3481 caaaaaatcg aattattgga atgggaggag ttttagacag ttcccgtttt aaaacctatt
     3541 tgagcatggc tctgggtgat gttaacctga acgacatcca ggcttgtgtg attggcggac
     3601 atggagacac caccatgatt cctcttaccc ggtacgccac ctacaagggc attccggttt
     3661 cggaatttct tagcgaagaa actttgaata aggttgcagc cgacaccatg gttggtggag
     3721 caacacttac caaactgctg ggcacttcgg cctggtatgc tccaggagct gccggagctt
     3781 acctggtaga atccattttg cacgaccaga aaaaggtgtt gccatgcagc gtttacctcg
     3841 aaggcgagta cgggcaaaaa gacatttgca taggtgttcc ggtagttatt ggtaaaaatg
     3901 gctgggagaa agtcgtcgac ttcaaactta acgccgcgga acaggctttg ttcgataaaa
     3961 gcgctgaggc tgttcggtct accaacagag tactggactc tgtactgtaa ttttatcgac
     4021 ggtgtctcat aagtgcccaa ctgctgcgtt actttcgata ttcaggctca gtcacatact
     4081 tcagtatgct cctttgccct caatctcagc ccttgcattt ggacgcttct aagacacctt
     4141 atgtttttgt gcttgaatag ttaattttga gacatcccct tttaattttt aaaatgctga
     4201 aaacatccga tatctgccgg aaaatggcgt ttagccttgt tgtgctgttt tgttgcggaa
     4261 cggcccaaag tttgtgtgcc cagcgggata gcatcgatca atccggcgaa cgttttatac
     4321 agcttttgca aacttccgaa aaaaacaagg gcacggtacg gatttttcaa gacaggcgca
     4381 ttgtagactt agttggcagt aagcaaatgt cggccaacat aatcaaagac ggagacaaaa
     4441 cc
//
LOCUS       DAOSTD010000116         4353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_18204_length_4353_cov_14.540484, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000116 DAOSTD010000000
VERSION     DAOSTD010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18204_length_4353_cov_14.540484"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="PLI37_07050"
     CDS             <1..1182
                     /locus_tag="PLI37_07050"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOQ58801.1"
                     /translation="RFILNNVRIGIEFHWEQEMQQCFEGALLIKTENHRLLAINRIAL
                     ERYLYSVIASEMNARAHTEFLKAHAVISRSWLLAQLGTNTQTAQIGPAAPENHPESPT
                     APENRTESPAAPENRSDSPAERIRWYEREAHTQFDVCADDHCQRYQGLLEVDTPQVKT
                     ALSSTQGMVLCSGEDICDARFSKCCGGLTESFESCWADTRLAYLVSKPDLIPAPDRSS
                     YADPEGFILGNPPSFCNTDDKGILEQVLKDYDFQTRDFFRWTVSYDARELSDLFKQRS
                     GLDLGLLSDLEALERGGSGRIVRLKVTGSKGSLIIGKELEIRRCLSKTHLYSSAFVVD
                     KVPDEQGKVRQFILHGAGWGHGVGLCQIGAAVMAEQGYKFEQILEHYYPNTQIKQLYD
                     F"
     gene            1172..2452
                     /locus_tag="PLI37_07055"
     CDS             1172..2452
                     /locus_tag="PLI37_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ58802.1"
                     /translation="MTSEKSISPWSWVPSLYFGEGLPYVAVMTISVILYKRFGMSNTD
                     IAFYTAWLYLPWTIKFLWSPFVDIIKSKRWWIVLMQLLIGAFFGGVAFSLPTPFWIQA
                     SMAFFWLTAFSSATHDIAADGFYMHALETKQQAFFIGIRNTFYRLAMITGQGLIIILA
                     GKLEQQTGNPFGMEQIPYAWMTVFLVMAVFFVLLGTYHAVVLPRPQSDTERKHLSNKQ
                     LRQELVQSIKSFFQKKHILTAIAFMLLYRFSEAQLVKIASPFLLDSAQAGGLGLNTAQ
                     VGFAYGTLGIASLVIGGIVGGIVISRRGLAASLWPMVLMLCLPNLIYVYFAFVRPESI
                     IVINLGVALEQLGYGYGFTAYTMYLLHFSQGPFKTAHYAICTAFMALGMMIPGMFAGA
                     LQEWLGYPLFFVWIVLATLPCFWVVALVKKQLKE"
     gene            2456..>4353
                     /locus_tag="PLI37_07060"
     CDS             2456..>4353
                     /locus_tag="PLI37_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOQ58803.1"
                     /translation="MLDIVTHIAGKLELGKEAVRNSLNLLDEGASIPFISRYRKENTG
                     GLNETQLTSIADLYEELKALEKRKESILQTIEEQGKLSPELKQQIEACTRKNELEDLY
                     LPYRPKRRTRAQIAREKGLEPLAALLMRQGPQDIGQLASRFTGSGLEVQEALDGASDI
                     IAEWVNENSRARHQVRNHFRRSALIRSVVVKDKESEGQNYRDYFDYSEPLRSCSSHRF
                     LAMIRGKNEGFLSISLKPDDEACLDSLERMFVRAKNPAGTLVAEAVKDAYKRLLKPSI
                     ENEFLSLSKEKADREAIRVFAANLKQLLLAPPLGQKRVLAIDPGYKSGCKVVCLDAQG
                     KLLHNENIFPHLDQRQKVLAAKKMAQLVESYQIEAIAIGNGTASRETEAFVKALRLDR
                     AVQVYVVSENGASVYSASAIAREEFPQYDVTVRGAVSIGRRLCDPLAELVKIDPKAIG
                     VGQYQHDVDQQMLKKALDQTVVHCVNAVGVNLNTASKHLLSYVSGLNSQSAARIVEYR
                     DENGAFNLRSELLKVPSVGPKSFEQAAGFLRIPGSDHPLDNTAVHPERYALVEKMAAG
                     LGCSTQDLIRNADLRKNIPLENYLDEHTGWPTLNDIMQELDKPGRDPRAKIEIFEFDP
                     NIRKFEDL"
BASE COUNT         1112 a         1160 c         1085 g          996 t
ORIGIN      
        1 cgctttattc ttaacaatgt ccgcataggc atagagtttc attgggagca ggaaatgcag
       61 caatgtttcg agggcgcctt gctgattaag acggaaaacc atcgcctgct ggccatcaac
      121 cgcattgccc tcgagaggta tttatacagc gttatcgctt ccgaaatgaa tgcccgggct
      181 cacacggaat ttctcaaagc ccatgccgtc atctcccgaa gctggttgct ggctcaactg
      241 ggcactaata ctcaaacggc ccaaatcggc ccggctgccc cggaaaacca cccagaaagc
      301 ccgactgccc cggaaaaccg cacagaaagc ccggctgccc cggaaaaccg gtctgacagc
      361 ccggctgaac gtattcgctg gtacgagcgc gaagcacata ctcaattcga tgtttgcgcc
      421 gacgatcatt gccaacgtta tcaaggcctt ttggaagtcg atactccaca ggtaaaaacc
      481 gcgctcagct ctacccaagg tatggtactt tgttcaggag aggatatttg cgacgcccgt
      541 ttctcaaaat gttgcggagg tctgaccgaa agcttcgaga gttgctgggc cgacaccaga
      601 ctagcctatc tggtatcaaa gcccgatctg ataccggccc ccgaccggag cagctatgcc
      661 gacccggagg gttttatact gggcaatccc ccctctttct gcaacaccga cgacaagggc
      721 atattggaac aggtgctcaa agactatgat tttcaaaccc gggacttttt tcgctggacg
      781 gtaagctacg atgcccggga gctttccgac ttatttaaac agcgttccgg attggacctt
      841 ggtttactga gcgacctgga agccctcgag agaggcggtt ccggacgaat agtccgcctt
      901 aaggtaacgg gcagtaaagg cagcctgatc atcggcaaag aactcgaaat acgacgctgt
      961 ttatccaaaa cccacctcta cagctcggct tttgtggtgg ataaagtacc ggacgaacaa
     1021 ggcaaagtcc ggcaattcat actgcatgga gccggctggg gacacggcgt aggactctgt
     1081 cagataggag ctgccgttat ggccgaacag ggttacaaat ttgaacaaat acttgaacat
     1141 tactacccta acacgcaaat aaagcaattg tatgacttct gaaaagagca tctctccctg
     1201 gagctgggtt cccagtctgt attttggcga aggcctgcct tacgtagctg tgatgaccat
     1261 ttcggtaata ctttacaaac gctttggcat gtccaacacc gacatcgctt tttacactgc
     1321 ctggctctac ctgccctgga ccattaaatt tctttggagt ccttttgtag atatcatcaa
     1381 aagcaaacgc tggtggatag tgctgatgca actgctgatc ggagcctttt tcggaggcgt
     1441 ggctttcagt ctgcccaccc ccttttggat tcaggccagc atggcttttt tctggctgac
     1501 ggctttcagt tcggccaccc acgatattgc agccgacggc ttttatatgc atgccctcga
     1561 aaccaaacaa caagctttct ttataggcat tcgcaatact ttttatcgcc tggccatgat
     1621 taccggtcag gggctgatta tcattctggc cggcaaactc gaacaacaga ccggtaaccc
     1681 ctttggcatg gagcaaattc cctacgcctg gatgacggtt tttttggtca tggccgtctt
     1741 tttcgtcttg cttggcacct atcatgctgt tgtcctgcct cgtccgcaga gcgacactga
     1801 acgcaaacac ttaagcaaca agcaattgcg tcaggaattg gtgcagagta ttaaaagttt
     1861 ctttcagaaa aaacacattc tgacggccat cgcttttatg ctgctctacc gtttttcgga
     1921 ggctcaattg gtcaagattg cctcgccctt tttgttggac agcgcccaag ccggaggcct
     1981 gggcctgaac actgcccagg tgggttttgc ctacggcacc ctgggcattg ccagcctggt
     2041 gattggcggc atagtgggcg gcatagtcat ttcccggaga ggactggctg ccagtttatg
     2101 gcccatggtg cttatgctct gcctgcccaa tctgatctat gtttactttg cttttgtccg
     2161 ccctgaatca atcattgtaa tcaatctggg ggttgctctg gagcaacttg gctacggtta
     2221 tggtttcacg gcctatacca tgtacttgtt gcatttcagc caggggcctt tcaaaactgc
     2281 ccattacgcc atttgtactg cctttatggc tttgggcatg atgattccgg ggatgtttgc
     2341 cggcgccctg caggaatggc tgggttatcc cttgtttttt gtttggatcg tgctggcaac
     2401 cctgccctgt ttttgggtgg ttgctctggt taaaaaacag ttgaaagaat aaattatgct
     2461 cgatatagtt acacatatag ccggaaagct cgaactggga aaagaagctg tcagaaacag
     2521 tctgaatctg ctggacgaag gagccagcat tccctttatc agccgttatc gcaaagaaaa
     2581 caccggggga ctgaacgaaa cgcaattgac gagtattgcc gacctgtacg aagagctcaa
     2641 agccctggaa aaacgcaaag aaagcattct gcaaactatt gaggaacaag gaaaactcag
     2701 tcctgaactg aaacaacaaa tagaggcctg tacacgaaaa aacgaactgg aagaccttta
     2761 cctgccctat cgtcccaaac gccgtaccag ggctcagata gccagggaaa aaggcctgga
     2821 gcccctggcc gccctgctga tgagacaagg accgcaagac attggacaac tagcctcccg
     2881 ttttaccggt tcgggcctcg aagtgcagga agctcttgac ggtgcttccg acatcatagc
     2941 cgagtgggtg aacgaaaaca gccgggcccg ccatcaggtc aggaatcact tcagacgcag
     3001 cgctctaatc cgctctgtag tggtcaaaga caaagaaagt gaaggacaaa actaccggga
     3061 ttatttcgat tacagcgaac ccctgcgttc ctgctcttct catcgctttc tggctatgat
     3121 ccggggcaag aacgaaggtt tccttagcat cagcctcaaa cccgacgacg aagcttgcct
     3181 cgacagcctg gaacgtatgt ttgtgcgggc aaaaaatccc gccggcacgc tggttgcaga
     3241 agccgtgaaa gatgcttaca aacgcctgct gaaaccttct atcgagaatg aattcctaag
     3301 tttgagcaaa gaaaaagccg accgggaagc cattcgggtt tttgccgcca acctgaaaca
     3361 attgctgctg gctcctccct tgggacaaaa gcgagtcctg gccatagacc ccggctacaa
     3421 aagcggttgt aaggtggttt gcctcgatgc acaaggcaaa ctcctgcaca acgaaaacat
     3481 ctttccccac ttggatcagc ggcaaaaagt gctggcggcc aaaaaaatgg cccaactggt
     3541 agaaagctat caaatcgagg ccatcgccat cggcaacggc acagcatcga gagaaaccga
     3601 ggcttttgtc aaagctttgc ggctcgaccg ggcagttcag gtgtatgtgg taagcgaaaa
     3661 cggagcttcg gtgtactcgg cttcggccat cgccagagaa gaatttcccc aatacgatgt
     3721 caccgtgcga ggcgcagtat ccataggaag gcgtttatgc gaccctctgg ccgaactggt
     3781 caaaatcgat cccaaagcca taggtgttgg ccaataccag cacgacgtcg atcagcaaat
     3841 gctgaaaaaa gccctggatc agacggtggt gcattgcgtc aatgccgtag gcgtaaacct
     3901 gaacacggcc agcaaacacc tgttgagtta cgtatcgggc ctgaatagcc agtcggccgc
     3961 ccgcatcgtc gaatacagag acgaaaacgg agctttcaat ctgcgcagcg aactgcttaa
     4021 ggtaccttcc gttggaccta aaagctttga acaggcagca ggtttcctgc gcatacccgg
     4081 ctcggatcat cccctcgata atacggccgt acacccggaa cgctacgccc tggtcgaaaa
     4141 aatggcagcc ggtttgggat gttccaccca agacctgatc cgtaacgcgg acctgcgcaa
     4201 aaacattccg ctggaaaact acctggacga acacaccggc tggcccaccc tgaacgatat
     4261 tatgcaagaa ctcgataaac cgggacgaga cccacgggca aaaatcgaaa tctttgaatt
     4321 cgatcccaac atcagaaaat tcgaggatct gca
//
LOCUS       DAOSTD010000117         4119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_19524_length_4119_cov_11.602362, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000117 DAOSTD010000000
VERSION     DAOSTD010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19524_length_4119_cov_11.602362"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PLI37_07065"
     CDS             complement(<1..949)
                     /locus_tag="PLI37_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58804.1"
                     /translation="MRRVALWLTAVFLLLGTGSKAQELLYDLELLSYFDNREYKVDFQ
                     RPQTILGMRIAPELGLGTADEKAGSHKLMLGLNYIQPLGAGIKEALVVPTVYYRYVHN
                     RFRVNLGAVPYSYLTESLPDYLMYDSLAYVHPNMQGGLFQYVAPRGYAEFLCDWRSAQ
                     SLERYEAFRLIIQARYQRGVLFGGGRAQLNHLANRARPAPRTGVSDDLIVHPYLGLDF
                     SRKLLLDSLSLQAGYLFGYQRDRSADQGTYLPRAFYLELFARWKRIGLKNVLYAGDNL
                     FPLYPVKGNLLNQGDPFYQAGFYNRTDAFVYLISTKRVNC"
     gene            complement(965..>1375)
                     /locus_tag="PLI37_07070"
     CDS             complement(965..>1375)
                     /locus_tag="PLI37_07070"
                     /inference="COORDINATES: protein motif:HMM:NF037182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOQ58805.1"
                     /translation="VIIQTSDPQHPVIQYVLNNDYRAFFQKECEERSKYAYPPFSRLI
                     EVIFRHKDESLLEQASVFFASLLPRDMESSVFGPMPALVARVKGLYLQKIILKIGPDI
                     SLKAAKDYLCRARDRFIKDKVYRQVQIYFDVDPV"
     assembly_gap    1376..1475
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1476..2824)
                     /gene="priA"
                     /locus_tag="PLI37_07075"
     CDS             complement(<1476..2824)
                     /gene="priA"
                     /locus_tag="PLI37_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOQ58806.1"
                     /translation="MKALYADVILPLAFGSGLSYSVPESLAENIRPGCRVRVPLGKQK
                     MYTGIVARLFDVPPTDINLKEIAGLIDDAPVVNAVQLEFWQWLFSYYLCTPGEVMKAA
                     LPAGLKGPRVAKAKTKSFLCLQPSLRDNPPAIEQTLTALARAKKQQEALRVFLRLAQT
                     SEHGPGLFREQLIEAGQQAAIIKALIQKNILEVHEIEVLPDRVPAEALRAAKQLSREQ
                     QKAMETIRQGWESGTNVCLLHGVTSSGKTEIYIHLILDYLAQGKQVLYLLPEIALTTQ
                     LTARLTEVFGNRLGIYHSACSDSERTHLWNKQLGDDPFDIILGVRSSVFLPFKNLGLV
                     VVDEEHENSYKQQDPAPRYHARNAAIVLASLHGAKTVLGTATPSIESYYNCRTGKYKL
                     VELSSRYLDWQLPEVMAVNTAELKRKKRMKSLLSPPLIAKMQETLEAGQQIMLFRNRR
                     "
     gene            complement(2821..3876)
                     /locus_tag="PLI37_07080"
     CDS             complement(2821..3876)
                     /locus_tag="PLI37_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOQ58807.1"
                     /translation="MNLAVVILLLLSAKMSVLSPERSVLSSYPNYAFLPLAFINLFFV
                     FYWALRKSVWFLMSLLSLLLCLPEQKAWFPVKLIKEQKKEDKTIKVLTYNTMQFSSYR
                     SHVKQDPNPVIRYLQESQADVLCLQEAGYSFDKQLLEKTTVITALKDYPYHSLEIIQS
                     NKYRTSNLWIFSKYPILKSGRLAYKSRSNASHYCDIKIGQDTLRFINNHLESNKLTKE
                     DMALYKIIVENPDRDNISQVAEKLGRKLGQAARLRAPQAETIAGFIRETPYKLVVCGD
                     FNDIPASYTYRCIKKGLRDAWSQNGRGLGITFHEKFYLFRIDYILHSPELRSYGTKVD
                     HVRYSDHYPVWTYLELP"
BASE COUNT         1068 a          959 c          929 g         1063 t
ORIGIN      
        1 agcaattgac ccttttggtc gatatcaggt aaacaaaggc atcggtgcgg ttatagaatc
       61 ctgcctggta aaaaggatct ccctgattga gcaaattccc tttgacagga tagagtggaa
      121 acaagttgtc gcccgcgtac aagacgttct tcaggccaat gcgcttccag cgggcaaaca
      181 attccaaata aaaagcccgg ggcaagtatg ttccctgatc tgccgagcgg tctcgctggt
      241 atccaaacaa atagcctgcc tgaagggaaa gagaatccag aagcagtttc cgtgaaaagt
      301 caagccccag atagggatgc acgatcaaat catcgcttac tcccgttcgg ggagccggtc
      361 tggcccggtt ggccaaatga ttgagctgag cccggccccc tccaaacaag acacctctct
      421 gataacgggc ctgtataatc agcctgaagg cttcgtaacg ctcaagactc tgagcgctgc
      481 gccaatcgca taaaaactcc gcgtagcctc ttggagccac atattgaaac aggccaccct
      541 gcatgttagg atgcacataa gccaaagaat cgtacattaa ataatcgggc agactctctg
      601 ttaaatagct ataaggaaca gcacccagat tcaccctgaa cctgttgtga acataacggt
      661 aatatacggt aggtacgacc aaagcttctt tgatgccggc gcctaaaggt tgtatgtaat
      721 tcaaacccag catcagttta tgactgccgg ccttttcatc tgccgtcccc aggcccagtt
      781 ccggtgcaat gcgcataccg agtatggttt gcgggcgctg aaaatcgacc ttgtattccc
      841 ggttatcaaa gtaagacagc agctcgagat cgtacaagag ttcctgagcc tttgaaccgg
      901 tgcccaaaag caaaaaaacc gcagtcaacc aaagagcaac tcttctcatg ttattatatc
      961 ttacttatac cggatcaaca tcaaaataga tttgcacctg acgatacact ttatctttaa
     1021 taaaccggtc cctggcccga caaagatagt ctttagcagc cttcaacgaa atatccggcc
     1081 cgattttaag aatgatcttt tgcagataga gcccttttac ccgcgccacg agagccggca
     1141 tagggccgaa aaccgaactc tccatatccc gaggcaaaag agaggcaaaa aaaacggagg
     1201 cctgctcaag caaactctca tccttatgcc tgaatatcac ctcaataaga cggctaaagg
     1261 gcggataggc atatttggaa cgttcctcac attctttttg gaagaaggcc cggtagtcgt
     1321 tgttcaaaac gtattgtatt acagggtgct gaggatcgga ggtctggatg attacnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgccg gttgcgaaaa agcatgattt
     1501 gctgcccggc ttcgagcgtt tcctgcattt tggctattag gggaggcgaa agcaacgatt
     1561 tcatcctttt cttgcgcttc aattcagcgg tattcactgc cataacttca ggcaattgcc
     1621 aatcgaggta tctgctggac agttcaacca gtttgtactt gcccgtacgg caattgtagt
     1681 aactttcgat gctgggtgtt gccgtaccaa gcactgtttt tgccccgtgc aaagaggcca
     1741 gcactatggc tgcattacgg gcgtgataac ggggagccgg atcttgttgt ttatagctgt
     1801 tttcatgttc ctcgtccact acgaccagtc ccaggttttt gaaaggcaaa aaaacagaag
     1861 aacgcactcc cagaataata tcgaagggat cgtctccgag ttgcttgttc caaagatgag
     1921 tcctttcgct gtcgctgcaa gcagagtgat atatccccag gcgattgccg aacacttctg
     1981 ttaagcgggc ggtaagctga gtagtaagag caatttcggg caagaggtag agcacttgct
     2041 taccctgcgc caggtaatcc agtatcagat gaatgtatat ctcggtttta cccgaagagg
     2101 tcacgccatg tagaaggcag acattggtgc cgctctccca gccttgccgt atggtctcca
     2161 tagctttctg ctgttcccgg ctgagctgtt tggcagctct cagagcctcc gccggcaccc
     2221 tatccggcaa cacttcgatt tcgtgaacct cgagtatatt cttttggatc aaagccttta
     2281 taatagccgc ttgctggccg gcctctataa gctgctctct gaataaaccg ggtccatgtt
     2341 ctgaagtttg ggctaaacga agaaaaactc taagagcttc ctgttgtttt tttgctcttg
     2401 ccaatgctgt cagtgtctgc tctatcgccg gaggattatc cctgagcgaa ggctgcaggc
     2461 ataagaaact tttagtcttg gctttggcaa cccgggggcc tttaaggccc gccggtagag
     2521 cggctttcat cacttcgccg ggcgtgcaga gataatacga aaacaaccat tgccaaaact
     2581 ccagctgaac ggcatttacc accggggcat cgtcgataag gcctgcaatt tctttcagat
     2641 tgatgtcagt gggtggcaca tcgaaaagcc gggctactat gccggtgtac attttttgtt
     2701 tacccagggg cactcttact ctgcatcccg gccggatatt ctccgccaga gattcaggca
     2761 cactgtaact cagacccgat ccaaaggcca aaggcagtat cacatcggca tacagagctt
     2821 tcatggcaat tccaggtaag tccaaacagg gtaatgatcg gaataacgta cgtgatccac
     2881 tttggtcccg taagatcgca actccgggga atgcagaata taatctatcc tgaacaaata
     2941 aaacttttca tgaaaagtaa ttccaaggcc ccggccattt tgcgaccagg catcgcgcaa
     3001 gccttttttg atgcagcggt aggtataaga agccggaata tcgttaaaat caccgcaaac
     3061 caccagttta tagggggttt cccgaataaa accggcgatg gtttcggcct gaggggctcg
     3121 caacctggcc gcctgaccca attttcgacc tagcttttcg gcaacctggc ttatattgtc
     3181 tctgtcggga ttttccacaa tgattttgta caaagccatg tcttctttgg ttaatttgtt
     3241 tgattccagg tgattgttaa taaaacgcag ggtgtcttgt cctattttaa tgtcgcaata
     3301 gtgtgaagca ttagatcggc tcttgtaggc taatctgccg gatttcaaaa tcggatactt
     3361 tgaaaaaatc cacaaattgg aggttctgta tttattagat tggatgattt ccaaactgtg
     3421 gtagggatag tctttgagtg cagtaataac agtggttttt tccaggagtt gtttatcgaa
     3481 gctgtaaccg gcctcctgca agcaaagaac gtcggcttgg ctttcttgta aatagcgaat
     3541 taccggattg gggtcttgct tgacatgaga acgataacta gaaaattgca tcgtattata
     3601 ggtcaacact ttgattgttt tatcctcttt tttctgctcc tttatgagtt tgaccgggaa
     3661 ccaggctttc tgttccggca aacagagcaa aagggacaat aaagacatta aaaaccaaac
     3721 acttttgcga agcgcccaat aaaaaacaaa aaaaagattt ataaaagcga ggggcaaaaa
     3781 agcataattc ggataagaac tcagtacgga acgttccggg cttaaaacgg acattttggc
     3841 actgagcaac agcagaatca caactgccag attcatcaga atcaatactg aatctcttaa
     3901 cacccgaaat atattcggtc ttttgggggc gtttttcatt gagccatttt caaggctgcg
     3961 gcggcaagcc ggggttatta attattgtcc ggatttgaac aaaaattgtt tttctttttt
     4021 gctcaggctt tcatagcctg attgcttgat tttatccagg atagcatccc tgtaatcttc
     4081 ttcgtttttt ttgcgatccc ggtattcctg atcgctttc
//
LOCUS       DAOSTD010000118         4006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_20244_length_4006_cov_16.205264, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000118 DAOSTD010000000
VERSION     DAOSTD010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20244_length_4006_cov_16.205264"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLI37_07085"
     CDS             complement(<1..219)
                     /locus_tag="PLI37_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58808.1"
                     /translation="MKSEEEDLLEYDDEKALSFIRNQLPQGMKSKHSDDDYYYLLDSI
                     YEYYENKGFLNDDEEEVTVDLDELSDFVY"
     gene            complement(249..1274)
                     /gene="recA"
                     /locus_tag="PLI37_07090"
     CDS             complement(249..1274)
                     /gene="recA"
                     /locus_tag="PLI37_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOQ58809.1"
                     /translation="MSKEQTEQKEQKGPAAEKLKALQMAMEKIEKSYGKGSIMKMGDD
                     KVVDVPVISSGSIGLNAALGVGGFPRGRVIEIYGPESSGKTTLAIHAIAEAQKAGGIA
                     AFIDAEHAFDRFYAEKLGVDIANLWISQPDSGEQALEITEQLIRSSAVDIVVIDSVAA
                     LTPKAELEGEMGDSKVGLQARLMSQALRKLTATISKTNTTCIFINQLREKIGIMFGNP
                     ETTTGGNALKFYSSVRLDIRRIGQIKDGEEIIGNQTRVKVVKNKVAPPFRKAEFDIMY
                     GEGISKTGELIDLGSDLGIIKKSGSWYAYKDSRLGQGREAAKDCMRDNPELAEEIEKL
                     IYEALKK"
     gene            1603..>2613
                     /locus_tag="PLI37_07095"
     CDS             1603..>2613
                     /locus_tag="PLI37_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOQ58810.1"
                     /translation="MVDIIFWLIPLASVTALIFAFYFYRQMKKKDEGTDLMKKIAGFV
                     REGAMSYLKQQYKVVTIVFIILALIFAAMAYFGLQNNWVPFAFLTGGFFSGLAGFLGM
                     KTATYASARTAHAARSSLNQGLQVAFRSGAVMGLVVVGLALLDISLWYLILNYFIDAA
                     SPEQKNMIITTTMLTFGMGASTQALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPR
                     NPATIADNVGDNVGDVAGMGADLYESYCGSILASAALGAASYQILSLEQQTKAILAPM
                     LIAAVGIFLSIIGIFLVKTKENAGVKSLLRSLAFGTNISAVLIAGASFLVLYLLGIEN
                     WLG"
     assembly_gap    2614..2713
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2714..3685
                     /locus_tag="PLI37_07100"
     CDS             <2714..3685
                     /locus_tag="PLI37_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOQ58811.1"
                     /translation="SGFKLGTFDFEHISSGLYGIGLASVGMLSTLGITLATDAYGPIA
                     DNAGGNAEMSGLEPEVRKRTDALDSLGNTTAATGKGFAIGSAALTGLALLASYIEEVK
                     IGLERLGTTTIEVGDKIIQTASASFSDFMAYYEVHLMNPKVLVGMFIGSMMTYVFCGL
                     TMNAVGRAAQKMVEEVRRQFREIKGIMTGKATPDYARCVEISTKGAQVEMLFPSLLAI
                     IVPTLTGLIFGVAGVMGLLIGGLSTGFITAIFMANSGGAWDNAKKYVEEGNFGGKGSE
                     AHKATVVGDTVGDPFKDTSGPSLNILIKLMSMVSIVMAGLTVAWSLL"
     gene            3791..>4006
                     /gene="rpsP"
                     /locus_tag="PLI37_07105"
     CDS             3791..>4006
                     /gene="rpsP"
                     /locus_tag="PLI37_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOQ58812.1"
                     /translation="MATKIRLQRHGRKGYAFYHIVAADSRAPRDGKFIERLGSYNPNT
                     NPATIDLNFDRALYWLETGAQPTDTVRN"
BASE COUNT          986 a          901 c          879 g         1140 t
ORIGIN      
        1 gtagacaaaa tcgctcaatt catcaagatc aacggtaact tcctcttcgt catcgtttag
       61 aaaacctttg ttttcgtagt actcgtaaat gctgtccaac aggtagtaat aatcgtcgtc
      121 gctgtgtttg gatttcattc cctgaggaag ttgattgcga atgaaactca aggctttttc
      181 gtcgtcgtat tccaacagat cttcttcttc acttttcata attgtgtgga tttaaggaat
      241 taaaaagctt attttttgag cgcctcgtaa attaattttt ctatttcttc ggccaattca
      301 ggattgtcgc gcatgcagtc tttggcggct tccctgccct gccccagtcg actgtctttg
      361 taagcatacc aggaaccgct ttttttgata atgcccaaat cggaacccag atctattaat
      421 tctccggttt tggaaatgcc ttcaccgtac atgatatcaa attccgcttt gcggaagggt
      481 ggagccacct tgttcttaac cacttttaca cgagtctgat ttccgatgat ttcttcaccg
      541 tctttaattt gtccgatacg acggatgtcg aggcgcactg aggaataaaa cttgagcgcg
      601 ttaccaccgg tagttgtttc gggattgccg aacatgatgc cgattttttc tctaagctga
      661 ttgataaaaa tacaagtcgt gttggttttg ctgatggtag cggtgagttt acgcaaagcc
      721 tgagacatca gacgagcctg aagacccact ttggaatcgc ccatttctcc ttccaattcg
      781 gccttggggg tgagagcagc tacagagtcg ataactacaa tatccaccgc tgaagagcga
      841 ataagttgtt cggtaatttc gagtgcctgt tctccgctgt cgggctgaga tatccacaaa
      901 ttggcaatat ctacacccag tttttcagcg taaaaacggt cgaaggcatg ttcggcatca
      961 ataaaagcag ctatacctcc ggctttttgc gcttcggcaa tagcatgaat ggccagggtg
     1021 gttttgccgg aggattccgg tccgtaaatt tcgattaccc ggccccgggg aaagccccca
     1081 acccccagag ctgcattcaa accgatggaa cccgaggaaa taaccggtac atcgacaact
     1141 ttatcgtctc ccattttcat aattgagcct tttccgtagc ttttttctat tttttccatg
     1201 gccatttgca gagctttcaa tttttctgct gctggacctt tttgttcttt ttgttctgtt
     1261 tgttcttttg acatatttgt taagattata atgtttacgg atcaaagata agacactact
     1321 ttgccaccgg caaacaaagt gctgttaaat actaataaat cattatgttt aaaattcttt
     1381 ttgtttaatt ttaatcttaa ttcctctatt aatgagtggc ttaaatgggt gtttattgta
     1441 ttcagccctc gaagcattct atgccttaca aagttataaa aaagatcggt taataagaca
     1501 ctatatactc tttaaataaa tgtcgtaaaa taaattccgc tcagatattg ccatttttgc
     1561 aaggagttta tacttttgtc cctactctct aaaaaaaaca ccatggtaga tattattttt
     1621 tggttaatcc cgctggcgtc agtcacagcc ttaatttttg ccttttattt ctacaggcaa
     1681 atgaagaaaa aagacgaagg aaccgactta atgaaaaaaa ttgccggttt tgtacgagaa
     1741 ggagcgatgt cctatctcaa gcaacaatac aaggtcgtca ccatagtatt tataatactg
     1801 gccttgattt ttgccgccat ggcctatttc ggtctgcaaa acaattgggt gccttttgcc
     1861 ttcctaaccg ggggattttt ctcgggtttg gccggctttc tgggtatgaa aacagccact
     1921 tatgcctctg cacgcaccgc acacgctgcc cgcagttccc tcaatcaggg cttgcaagtg
     1981 gctttccgaa gcggggccgt catgggtctg gtggtcgttg gtcttgcctt gctggatatt
     2041 tcgctctggt atctcatttt gaattatttt atagatgcgg ccagcccgga gcaaaaaaac
     2101 atgatcatca ccaccaccat gctgactttt ggcatgggtg catctaccca agctttgttt
     2161 gcccgcgtgg gcggaggtat ttacactaag gcagccgacg taggtgccga cctggtgggc
     2221 aaagtggaag ccggaatccc ggaagacgat ccccgcaacc cggctaccat tgccgacaac
     2281 gtgggcgata acgtgggcga tgtggccggt atgggagccg acctttacga atcttattgc
     2341 ggctccatcc tggcttcggc cgccctgggt gcagcctcgt accagattct cagcctggaa
     2401 caacaaacca aagctattct agctcctatg ctgattgccg ccgttggaat cttcttatcc
     2461 atcattggta tttttctggt caaaaccaaa gaaaacgcag gggtcaaatc cttgttacgt
     2521 tcccttgctt tcggcaccaa catcagcgca gtacttattg cgggagccag tttcctggtt
     2581 ctctatttac tgggaatcga aaactggctg gggnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnntcgggtt tcaaactggg cactttcgat tttgaacata tttccagtgg
     2761 tttatatggt attggattgg cgtctgtggg tatgctctct acccttggta ttactctggc
     2821 taccgacgct tacggaccca tcgcagacaa tgccggaggt aatgccgaga tgagcggtct
     2881 tgaacccgaa gtgcgcaaac gcactgacgc cctcgattca ctgggcaata cgaccgcggc
     2941 gaccggcaag ggatttgcca ttggctcagc cgcattgacc gggctggcac tgttggcttc
     3001 ctacatcgaa gaagttaaaa tcggcctgga aaggcttggt actaccacca tagaagtagg
     3061 agacaaaatc attcaaacag ccagtgccag ctttagcgac tttatggctt attacgaagt
     3121 acatttaatg aatcccaaag tgctggtggg aatgttcatc ggttccatga tgacttatgt
     3181 tttctgtggc ttaaccatga atgccgtagg cagggccgct caaaaaatgg tcgaagaggt
     3241 caggcgtcaa ttccgtgaaa ttaaaggtat catgacaggt aaagccactc ccgattacgc
     3301 ccgctgcgtg gaaatatcca ctaaaggcgc ccaggttgaa atgcttttcc cctccttgct
     3361 tgccattatt gttcccacat taaccggatt gatctttgga gtcgccggag ttatgggttt
     3421 attgatcgga ggtctgagta ccggttttat tacggctatt tttatggcca actccggagg
     3481 agcctgggac aacgccaaaa aatacgtcga agaaggaaat ttcggaggca aaggctccga
     3541 agcccacaaa gccacagtgg ttggcgatac cgtcggcgat cctttcaaag acacctcagg
     3601 acccagtttg aacattctta tcaaactaat gagtatggtt tccattgtga tggccggttt
     3661 aacagttgcc tggagtttgt tgtagttcct ttgttaaggc taaaaaatat ggactgtttt
     3721 gcattaaaat aaacaatttt gcctaccttt gcggccgcta atgcaaaact tataattcat
     3781 ttcaaaccaa atggcaacaa aaatcagatt gcaaagacat gggcgaaaag ggtacgcgtt
     3841 ttaccacatt gtcgccgcag acagcagggc accacgagat ggtaagttta ttgagagatt
     3901 aggttcgtac aacccgaaca caaatcctgc tacaatagat ttgaacttcg atcgtgcttt
     3961 gtattggctg gaaaccggtg ctcaacccac cgacaccgtt cgtaac
//
LOCUS       DAOSTD010000119         3997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_20297_length_3997_cov_13.350583, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000119 DAOSTD010000000
VERSION     DAOSTD010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20297_length_3997_cov_13.350583"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLI37_07110"
     CDS             <1..558
                     /locus_tag="PLI37_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58813.1"
                     /translation="RNNSATIYHGFVQLLELSGLLDFDSQSVLPVVESCLMFVPSTAA
                     VKQAVQEGRLPGVVSGSATVDDAGYFGKCTVTNADSLQYYLLKYFIPMSTAVISNYPY
                     VGWEENTAEGLPTLQSYDEPVEGGKVVTITTKIDVSDAGGKLSVQELDNDGNPVGERV
                     DVVEDYHYFPFIFDDACVHFIKEVL"
     gene            581..>2484
                     /locus_tag="PLI37_07115"
     CDS             581..>2484
                     /locus_tag="PLI37_07115"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOQ58814.1"
                     /translation="MTKKIVYTLIVFLLAATGALHAQDYITGKVYELAGNERIVFPGV
                     NVMVMNEQNRLLNGTTTNQNGEYSIRIPQVTGKLKVVFSFIGMETQRFDYTGQKTLDV
                     TLKEEAMMLAEMVVSAERLEKNELGITAREQTFATQSIKMEDFIESLPVTSVEEALQG
                     RMSGVDIVAGGDPGAKSSIRIRGTATLNSNADPLIVINGVPYTTDIDDSFDFATANNE
                     DYADMLNLNPHDIESIEVLKDAASTSVYGTSGANGVLLITTKKGGRGKTRFSLSSKFT
                     AKVEPNPIPMLDGDEYVAFIQDAIWNTANARGVANSSSLLELLFDTPEINYNPDWRYF
                     DEYNADTDWLSYVKKNATISDNSFSMSGGGDKATYRFSLSYLNEGGTTLGTSLQRVTS
                     SLNIGYNFSDKLRVDADFTYSDTDKEDNWTTAVRAEALRKMPNKSPFWIDDLTGEPTS
                     NYFIRQNSEEFQGAFKGSSNFHPLIMVNDSYNNSDQKEEKMTFRMNYDISTGLIYTGY
                     VSLKFRTVNNRKFLPQAATDVTIDNNYANRSTDAYSSNLSLQTENKLIFRKNWEGLHN
                     LVATGLWRTSQSVSSNYSTEIYGASSAGMSDPVTGGAIRGLGSGDSEVRTLSGIGNFN
                     YTLLNRYIING"
     assembly_gap    2485..2584
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2585..3583
                     /locus_tag="PLI37_07120"
     CDS             <2585..3583
                     /locus_tag="PLI37_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOQ58815.1"
                     /translation="TKDLLQTGVSIPSSTGYDGKGNTISYFNSGELSNRGWEYRIDYD
                     VYRDTDWRISLNFNVNRNINRIELLPENLSESTYTLSNGSYAQKIIAGMPVGSFFGFH
                     YEGVYQNLEDTYAKDEDGRIMLDLQGEPIVMKNGTFTCYPGDAKYQDVNYDGLINEKD
                     ILYLGNSMPVVTGGGGFNVKYKNLSLITFFHYRLGQKIINKARMESEAMNGTDNQSKA
                     VLKRWRNEGDDTIIPRALWKYGLNYMGSDRFVEDCSFLRLKTLSLSYNLPKDYCQRLR
                     TNSINFYVTAYDLFTWTNYTGQDPEVNLPTKITSLAMDNSQTPRSKRYSCGISVNF"
     gene            3594..>3997
                     /locus_tag="PLI37_07125"
     CDS             3594..>3997
                     /locus_tag="PLI37_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ58816.1"
                     /translation="MKRMKNIGLLLSIAFLLLSCNEWLQVDPQNEQLSYKYWSSQEDV
                     EAVVNSGYYLLREMVTDYLIPFGELRSGQLFSIRSNKLQSFQVKAIDKQFSNWGPFYE
                     IINIANVVLANAHLAESIDDTYEVEEMNAHHC"
BASE COUNT         1068 a          900 c          957 g          972 t
ORIGIN      
        1 agaaacaact ctgcaaccat ttatcacggt tttgtgcagt tgctcgagtt gtcgggcctg
       61 ctcgatttcg attctcaatc ggtgttgccg gtggtagaaa gctgcctgat gttcgtgcct
      121 tccacagcag ccgttaagca agccgtacaa gagggccggc tgcccggcgt tgtcagcgga
      181 agcgccacgg tagacgatgc ggggtatttc ggcaaatgca cggtgaccaa cgccgattcc
      241 ctgcaatatt atcttttgaa atattttatc ccgatgagta ccgcagtgat ttcgaattat
      301 ccttatgtag gctgggaaga aaacaccgca gagggcctgc caaccttgca gagttacgat
      361 gagcctgtgg agggtggaaa ggtggtgacc attaccacca aaatagatgt gagcgatgcc
      421 ggaggcaagc tgagcgtgca agagttggat aacgacggta atcctgttgg agaaagggtt
      481 gatgtggtcg aggactacca ttatttcccc tttatcttcg atgatgcctg tgtccatttt
      541 attaaggagg tattgtaaac ccggctagaa agattctgtt atgacaaaaa aaatagttta
      601 tactctgatt gttttcttgc tggctgccac cggagcgctc catgcgcagg actatatcac
      661 gggcaaggtt tacgaattgg cgggcaacga aagaattgtt tttcccggcg tcaacgtaat
      721 ggtgatgaac gaacaaaatc gtttgttgaa cggtaccacc accaatcaaa acggagaata
      781 ttccattcgt attccacaag tgaccggcaa attgaaggtg gtattttcct ttatcgggat
      841 ggagacccag cgctttgatt acacaggaca gaaaacgctg gatgtgacct tgaaggaaga
      901 agctatgatg ctggccgaaa tggtggtatc cgcggaaaga ttggaaaaga atgagttagg
      961 tattactgcc agagagcaga cctttgccac tcaaagcata aagatggagg atttcataga
     1021 atccttgccg gtgacttcgg tagaggaagc cctgcaggga cgaatgagcg gggtggacat
     1081 cgttgccgga ggcgaccccg gagccaaaag ttccatcagg attcgcggaa cggccaccct
     1141 gaattccaat gccgatcccc tgatcgtaat caacggagtg ccctatacca cggatatcga
     1201 cgattctttt gactttgcca cggccaataa cgaagattac gcggatatgc tcaatctcaa
     1261 ccctcacgac atcgaaagta tagaggttct caaggacgct gcctccacct cggtttacgg
     1321 aaccagcgga gccaacggag tgttgttgat taccaccaaa aaaggaggca gaggcaaaac
     1381 ccgtttttca ctttcttcga agtttacggc caaagtggag cccaatccta ttcctatgtt
     1441 ggacggcgac gaatacgtcg cttttattca ggatgccata tggaataccg ccaatgcccg
     1501 gggtgtggcc aattcttcct ctttgctcga attgcttttc gataccccgg aaatcaatta
     1561 caatcccgac tggcggtatt ttgacgaata caatgccgat accgactggt tgtcgtatgt
     1621 gaagaaaaac gccaccatct ccgacaatag tttttccatg tcggggggcg gcgataaagc
     1681 cacttaccgt ttttcgcttt cttacctgaa cgagggtgga accaccctgg gaacttcgct
     1741 gcaaagggtt acttcgagcc tgaacatcgg ctacaatttc tcggacaagt tgagggtgga
     1801 cgccgatttc acctattccg acaccgacaa ggaggacaac tggaccactg ccgtcagggc
     1861 tgaggctttg agaaaaatgc ccaacaaaag tcctttttgg atcgacgatt tgacaggcga
     1921 gcctacttcc aattatttca ttcgccaaaa cagcgaggaa tttcagggcg cattcaaagg
     1981 ttcttccaac tttcaccctc ttatcatggt aaacgacagc tacaacaaca gcgatcagaa
     2041 agaggaaaag atgactttta ggatgaacta cgatatcagt acaggtttga tttacaccgg
     2101 ttatgtgtcg ctaaagttta gaacggtaaa caacagaaag tttttgcctc aggcggctac
     2161 cgatgtcacc atagacaata attacgctaa ccgtagtacc gatgcctatt cgagtaattt
     2221 atcgctgcaa accgaaaaca aactgatttt ccgcaaaaat tgggaaggat tgcacaacct
     2281 ggtggccaca ggcctttgga gaacctcaca gtcggtgagc tccaattatt cgaccgagat
     2341 ttacggagcc tcatcggcgg ggatgtccga tcccgtcacc gggggcgcca tacgcggctt
     2401 gggttcgggc gattccgagg taagaacctt gtcgggtata ggcaacttca attatacttt
     2461 gcttaaccgc tatattatta acggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnaccaaa gacttgttgc agaccggtgt ttccattcct tcatctaccg ggtacgacgg
     2641 caagggcaat accatctcgt acttcaattc gggagaactg agcaaccggg gctgggaata
     2701 ccgcatcgac tacgatgtat atcgtgatac cgactggaga atcagcctta attttaatgt
     2761 caaccgtaat atcaaccgca tagagttgct gcccgaaaac ttatcggaat caacttatac
     2821 tctgtccaac gggagttatg cccagaagat tatagccggt atgccggtgg gttctttttt
     2881 tggcttccat tatgaagggg tgtatcagaa cctcgaggac acttatgcca aggacgaaga
     2941 cggccggata atgctcgact tgcagggaga acctatagta atgaaaaacg gaacctttac
     3001 ttgttatccc ggcgacgcca aataccagga tgtcaattac gacggtctga tcaacgaaaa
     3061 ggacattctc tatttgggta attccatgcc tgtggttacc ggtggcggcg gcttcaatgt
     3121 gaaatataaa aacctgagct tgattacttt cttccattat cgcctggggc agaaaataat
     3181 caacaaagcg aggatggaaa gcgaagccat gaatggcacc gacaatcaga gtaaagccgt
     3241 tttgaaacgc tggcgcaacg aaggcgacga tacgatcatt cccagagctt tgtggaaata
     3301 cggcctgaac tacatgggtt cggatcgctt tgtggaagac tgttcttttt tgaggctcaa
     3361 aaccttgtcg ctcagttaca atttacccaa agactattgt cagaggctta ggaccaacag
     3421 catcaatttt tatgtgaccg cttacgacct gttcacctgg accaattaca ccggacaaga
     3481 tcccgaagta aacctgccca ccaagattac cagcctggct atggacaatt cccagacccc
     3541 gcgaagcaag cgttattcct gtggtatttc cgtcaatttc taagattagt acaatgaaaa
     3601 gaatgaagaa tatcggcctt ttgcttagca ttgccttcct gttgctttct tgtaacgagt
     3661 ggttgcaggt cgatcctcag aacgagcagt tgagttataa atattggtct tcgcaagaag
     3721 acgtggaagc cgtggtcaat tccggctatt atttattgcg cgaaatggtg accgattacc
     3781 tgattccttt cggtgaattg cgcagcggtc aactgttcag catcagaagc aataaattac
     3841 aaagctttca ggtgaaagcc atagataaac aattcagcaa ttggggtccc ttttacgaaa
     3901 tcatcaatat tgccaatgtg gtgctggcca atgctcacct ggccgagagt atcgacgata
     3961 cttacgaagt ggaagaaatg aacgctcatc attgcga
//
LOCUS       DAOSTD010000120         3850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_21297_length_3850_cov_10.355468, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000120 DAOSTD010000000
VERSION     DAOSTD010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21297_length_3850_cov_10.355468"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PLI37_07130"
     CDS             <1..752
                     /locus_tag="PLI37_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58817.1"
                     /translation="FAHSSIVVLQRIKHHIHYYLHARHRRGHGVHPPFAYRLITAIIE
                     QEHPYYCFDELEAGRARMEYERIRLETKRTRLETRHAVRNAKGKGLDAECSGREARQS
                     DSGVRSGKHCLSPADAQLLFRLVNAFALHKVMEWGGIDETTRLYLTRASSKVKIYRGG
                     PDIGVIPDLLFCNADLDEAALSQALSQALSQKTEQSLLVLAGPHRSAGAYRCWQQLIT
                     HSEVRLSVEMKGLGLVFFDPELQKDHYILRR"
     gene            complement(820..1906)
                     /gene="prfB"
                     /locus_tag="PLI37_07135"
     CDS             complement(join(820..1842,1844..1906))
                     /gene="prfB"
                     /locus_tag="PLI37_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629026.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOQ58818.1"
                     /translation="MITTDQIKEIQEREHALRRYLDIENKIDALKEEELKTQAPGFWE
                     KQKEAEEQMRKIKQLKGWIEDYEEVQTAVQELTLAYDFYKEEALSEKEMDEQYDRTLN
                     LLENLEMKNMLRHEEDKLGAVLKINAGAGGTESQDWAEMLYRMYQRYCDKQGYKTNIT
                     NWQDGDEAGIKTVTMTVEGDYAFGYLKSENGVHRLVRVSPFNAQGKRMTSFTSVFVVP
                     LVDDSIEIEVKDSDITWDTFRSSGAGGQNVNKVETGVRLHHLPTGIVIENTESRSQLG
                     NKENALRLLKSQLYEMELEKRRAEQAKIEAGKKKIEWGSQIRSYVFDDRRVKDHRTDH
                     QTSNVQAVMDGELDGFIKAYLMEYGAE"
     gene            complement(1906..3726)
                     /locus_tag="PLI37_07140"
     CDS             complement(1906..3726)
                     /locus_tag="PLI37_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOQ58819.1"
                     /translation="MNIYHPSRLVSEYAASYGDRIAMRYRDYTDGRWKGISWKELNGQ
                     VNYLCAALLDFGIKEHEKVGIFAQNMPECIVSEFSIFSLRAVSVPMYATSTTSQIEYI
                     ISDAGIRLMFVGEQYQYDRAFEALSHRECGLKQIVVFDTNVRLQKNDDHSIYFKDFVH
                     KGMLLNLGDSVEVKRSECRDDDLANILYTSGTTGEPKGVQLLHSNYQEIVRVMDIKLL
                     HLFDKNEVSMCFLPLTHIFEKAWSLYCFSRLIRVDINLKPLDIQRSLKEVRPTIMCNV
                     PRFWEKVYSGIMEKIDAMPALAQKWVFHAVKLGKRYHLDYRRLDIKPPFWLSLRYNLY
                     RVSLFTILKKAVGLENGKLFPVSGAKLSDDILIFMRSVGIPICYGYGLTETTATVASF
                     DEKHFSLGSVGSFIDKLQVRIDDNGEILLKGETITPGYYNKPEINAKAFTEDGFFRTG
                     DMGYINKEGHLVLTERLKDLFKTSNGKYIAPQSIEAVLCNDKYINMVAVIGDQRKFVS
                     ALIVPAYEELKNLAVKLGIPDLDADKLEDVLKDERIYAFFENRIRMLQKNMAGYEQIR
                     RFVLLPKPFSVADGELTNTLKMKRNVIADHYKDEIEKMYL"
BASE COUNT          888 a          927 c          928 g         1107 t
ORIGIN      
        1 cctttgcgca ttcatcgatt gttgttttgc aacgtataaa acaccatata cactattatt
       61 tacacgcgcg gcaccgccgg ggtcacggag tgcatccccc ttttgcttac cggctgatca
      121 ccgctattat cgaacaagag catccttatt attgtttcga cgaactggaa gccgggcgag
      181 cccgcatgga atacgaacga atccggttgg aaacaaagcg aacccgcctc gaaacccgac
      241 acgctgtacg gaacgccaaa gggaaaggac ttgatgccga atgctccggc cgggaagccc
      301 ggcagtcgga ttccggcgtc cgttccggga agcattgtct gtctccggcc gatgcccaac
      361 ttttgttccg gctggtgaat gcctttgctt tgcacaaggt aatggagtgg ggaggaatag
      421 acgaaacaac ccggctctat ttaacaagag cttcttctaa agtaaaaatc tatcggggag
      481 ggccggatat tggcgtgatt ccggatttgc tgttttgtaa cgcggacctt gacgaggcgg
      541 ccttgtcgca ggctttgtcg caggccctga gtcagaaaac ggagcagagc cttttggtgc
      601 tggccggccc gcatcgttcg gcgggagcgt accgctgctg gcagcaacta ataactcatt
      661 ccgaagtgcg actttcggtg gagatgaagg gtttggggct ggtgttcttc gatccggaac
      721 tacaaaagga ccactatatt ttgaggcgtt aagtttgttc ggctccgtat tgtatcaaac
      781 agcttgtaaa aatcgaggaa gttgaaggaa acgactagtt tattcggctc cgtattccat
      841 caaataagct ttgatgaatc catccagttc gccgtccata actgcctgta cgttggaggt
      901 ctgatgatcg gtgcggtggt cttttacccg gcggtcgtcg aaaacatagc tgcggatttg
      961 ggaaccccat tcgattttct tcttcccggc ttcaattttg gcttgctcgg cccggcgttt
     1021 ctcgagctcc atctcgtaaa gttgcgattt gagcagcctc agggcatttt ctttgtttcc
     1081 cagttgagaa cggctttcgg tgttttcgat cactatgccc gtgggcaggt gatgcagacg
     1141 aacccctgtc tctaccttgt tgacgttctg gcctccggcg cccgaggagc gaaaggtgtc
     1201 ccaggtgatg tcggaatcct tgacttcgat ttcgatgctg tcgtccacca atggaaccac
     1261 aaacaccgag gtaaaggaag tcattcgctt gccctgggca ttaaaggggg aaacccgcac
     1321 caggcgatgc accccgtttt cgctttttaa ataaccaaaa gcgtaatcgc cctcgacggt
     1381 catggtgacg gttttgatac cggcctcgtc tccgtcctgc cagttggtga tgttggtttt
     1441 gtagccttgt ttatcgcaat agcgctgata catgcggtag agcatttcgg cccagtcctg
     1501 gctttcggtg ccgcccgctc cggcatttat tttgagtaca gccccgagtt tgtcttcttc
     1561 gtggcggagc atatttttca tttccaggtt ttcgagcaga ttcagggtac gatcgtactg
     1621 ctcatccatt tccttttcgc tcagggcctc ttctttgtaa aaatcataag ccagtgtcag
     1681 ttcctgtacg gcagtttgaa cttcttcgta atcttctatc cagcctttga gttgcttgat
     1741 tttgcgcatt tgctcctcgg cttctttttg cttttcccag aaaccgggag cctgggtctt
     1801 gagttcttct tcttttaaag cgtcaatttt gttttcgatg tcaaagatac ctcctcagcg
     1861 cgtgctcgcg ctcctggatc tctttgatct ggtctgtagt aatcattata aatacatttt
     1921 ttctatttca tctttgtaat gatcggcaat tacgttgcgt ttcatcttaa gcgtgttggt
     1981 caattcgccg tcggctacac tgaaaggctt gggtaataaa acaaaacggc ggatttgttc
     2041 gtaaccggcc atgttttttt gcaacatgcg gattctgttc tcaaaaaagg catatatccg
     2101 ctcgtcttta agcacatctt cgagcttatc ggcatcaaga tcgggtatgc ccagttttac
     2161 cgccaaattt ttaagttcct cataagcggg cacaatcagg gcgctgacaa attttcgctg
     2221 atctccgatg acggcaacca tgttgatgta tttgtcgttg catagcaccg cttctatact
     2281 ttggggagct atgtatttgc cgttggaagt tttaaacaaa tcctttaggc gctcggtcag
     2341 caccagatgt ccttctttgt tgatgtaacc catatcgccg gtgcggaaaa aaccatcttc
     2401 ggtaaaggct ttggcattga tttcgggttt gttgtaatac cctggagtga tggtttcgcc
     2461 tttgagcagg atttctccgt tgtcgtcaat gcgcacttgc aacttatcga taaaactgcc
     2521 cacactgccg agactgaaat gcttttcgtc aaagctggcc accgtagctg tggtctcggt
     2581 cagaccataa ccataacaga tgggtatgcc cactgagcgc ataaaaatca gaatgtcgtc
     2641 cgacagtttg gctcccgaaa cgggaaaaag cttgccgttt tccaggccta ccgccttctt
     2701 gagaatggta aacaagctta ctctgtaaag gttatagcgc agactcagcc aaaaaggagg
     2761 cttaatatcg agacgcctgt aatcgaggtg gtatctttta cccagtttga cggcatggaa
     2821 aacccatttt tgagccaagg caggcatagc gtcgattttc tccataatgc cgctgtacac
     2881 tttctcccaa aaacggggca cgttgcacat gatggtggga cgaacctctt tgaggctccg
     2941 ctggatatcg agcggtttaa gattgatgtc cacccggatc agcctcgaaa agcaatacag
     3001 actccaggct ttctcgaata tatgggtcaa gggcaggaaa cacatcgaaa cctcgttttt
     3061 atcgaagaga tgcaagagtt tgatgtccat cacccttacg atttcctggt aattgctgtg
     3121 caggagttga acgcctttgg gttcgccggt ggtacccgaa gtgtaaagga tgttggccag
     3181 atcatcgtcg cggcattcac tgcgtttgac ctccaccgaa tcccccaagt taagcagcat
     3241 tcctttgtgt acaaagtcct tgaaataaat cgagtgatcg tcgtttttct gtaaacgcac
     3301 attggtatcg aaaacaacga tttgtttcag tccgcattcc cggtggctaa gggcttcgaa
     3361 agcccggtcg tattgatatt gttcgcctac aaacatcagc cggatacctg cgtcggaaat
     3421 gatgtattca atctgactcg tagtgctggt ggcgtacatg ggcacgctta ccgcccttag
     3481 gctgaaaatg gaaaactcgc tgacgataca ttcgggcata ttttgagcaa aaatgcccac
     3541 tttttcgtgt tctttaatac caaaatccag taaggcggca cataaataat tgacctgacc
     3601 gttcagttct ttccacgaga tacctttcca gcgaccgtcg gtatagtccc ggtagcgcat
     3661 ggcaatccga tcaccgtaac tggctgcgta ttcggacacc agtctggaag gatgataaat
     3721 attcatttga tatagaattg attcgacctg caaaagtacg cttataattc atattttttt
     3781 tcaaaagctc caagttttgt attgtttaaa gatattctta gctaattttg catgttgatt
     3841 atcaataatt
//
LOCUS       DAOSTD010000121         3838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_21385_length_3838_cov_13.744647, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000121 DAOSTD010000000
VERSION     DAOSTD010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21385_length_3838_cov_13.744647"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLI37_07145"
     CDS             complement(<1..214)
                     /locus_tag="PLI37_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ58820.1"
                     /translation="MLEIKNLHASAGDKEILKGLNLSVKEGEIHAIMGPNGSGKSTLS
                     AVLSGNPAFTVTQGTVRFKGMDLLSMT"
     gene            complement(270..620)
                     /locus_tag="PLI37_07150"
     CDS             complement(270..620)
                     /locus_tag="PLI37_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58821.1"
                     /translation="MKSKRETKTAQNRGTDDNEAPIQKSRGAIWVAIFILLILAGLGY
                     LLFSTLPLEEEGNRQRLLVSALALMLLVVGLLVFVQGWFKKTTRKKGKAKRKPSRYSP
                     APKSVKRGKKTGRR"
     gene            complement(583..1215)
                     /gene="pnuC"
                     /locus_tag="PLI37_07155"
     CDS             complement(583..1215)
                     /gene="pnuC"
                     /locus_tag="PLI37_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOQ58822.1"
                     /translation="MNLLELIGLVSGLVAVYLASRALSVNFLFGLLNNVVYFVVFYQA
                     HLYSAMLLQVVYFSFSLYGYYHWKHPAPGQEGKNRELRIRRLNKSQWALVIVIVLVFG
                     GLWSEGIIYFQSRFPEYIDPPAFPRLDAMLTAASVMGQFLLSRKVKDNWIIWIVVDVL
                     STALYAYMGLFFTALLFAVFTLIALRAIVDWNKQYKQQDEIKKGNKNRTK"
     gene            complement(1262..2713)
                     /gene="sufB"
                     /locus_tag="PLI37_07160"
     CDS             complement(1262..2713)
                     /gene="sufB"
                     /locus_tag="PLI37_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOQ58823.1"
                     /translation="MKSTEDKDIITQVTAGEYKYGFVTDIETETIPEGLNEDVIRLIS
                     SKKMEPDWLLEFRLKAFRHWQSMEKPQWAHLDIPEIDYQKMVYYAAPKSKTSGDNEED
                     IDPDLLKTFEKLGIPLQERKQLAGMAVDAVMDSVSVKTTFKESLAKKGIIFCSFSEGV
                     QEHPELIKRYLGSVVPYTDNYYAALNCAVFSDGSFVYIPKGVRCPMELSTYFRINAIN
                     TGQFERTLIVADDDSYVSYLEGCTAPIRDENQLHVAIVEIVVHDRAEVKYSTVQNWYP
                     GDKEGKGGIYNFVTKRCLCKGESAKISWTQVETGSAITWKYPSCILAGDNSVGEFYSV
                     AVTNNHQQADTGTKMIHLGKNTRSIIVSKGISAGKSQNSYRGLVKIAPKASNARNHSQ
                     CDSLLLGDTCGAHTFPYIEINNETAVVEHEATTSKINEDQLFYCNQRGISTEEAIGLI
                     VNGYAKEVINKLPMEFAVEAQKLLQVSLEGSVG"
     gene            complement(2717..3262)
                     /locus_tag="PLI37_07165"
     CDS             complement(2717..3262)
                     /locus_tag="PLI37_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOQ58824.1"
                     /translation="MNLYDIIILIVALLGLIDGLRRGLIQTIGTIAGVVCGIFAAKAL
                     SYLVAPYISQLFESASLKVVQVVSFIVLFVLTVILFHFVAVLLGKLVKMVMLGWLNRI
                     AGGLFAVVLYLFVLSIVVNVFDYFDKGKSSFHREKSSTSRFYGPLSDFAPAVLPFIHF
                     EDWNTKKPETLQQSTPVPQSI"
     gene            complement(3266..>3838)
                     /locus_tag="PLI37_07170"
     CDS             complement(3266..>3838)
                     /locus_tag="PLI37_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOQ58825.1"
                     /translation="SIEALSDSLIGLSTEQIQVNVIHKAVGQISESDVTLASASDAII
                     VGFQVRPSMAARKMAERDGVDIRLYSIIYDAIEEIKSAMEGMLSPEIKEEVTATAEVR
                     EVFRITKVGTVAGCMVKEGKIKRGTKIRLIRDGIVVFTGELSSLKRFKEDVKEVASGY
                     ECGLSINNYNDIKVGDLIEAYEEIEVKQTL"
BASE COUNT         1074 a          967 c          748 g         1049 t
ORIGIN      
        1 gagtcatact taaaagatcc atgcctttga agcgaaccgt accttgggta acagtaaagg
       61 ccggatttcc cgaaagtaca gctgaaagcg tacttttccc cgagccattg ggtcccataa
      121 tggcatgtat ttctccttct ttcaccgata aattcaggcc tttcaggatt tctttatctc
      181 ctgcggaagc atgtaaattt tttatttcca acaacataaa tatcgtcttt acggccttgg
      241 ccaatctgtt tttattcttt gtttgttttc tatctcctcc cggtcttttt tccacgtttt
      301 acggacttcg gcgcaggaga ataccttgat ggctttcttt ttgctttacc ctttttcctc
      361 gtcgtttttt tgaaccagcc ctgaacaaac accaataatc ctacaaccag caacatcaag
      421 gcaagggcac tcaccaaaag ccgctgcctg tttccctctt cttccaaagg cagggtacta
      481 aacaacaaat aacccaagcc tgccaggatc aagagaataa agatggcgac ccaaatggct
      541 cctctgctct tttgaatagg agcttcgttg tcgtcagttc ctctattttg tgcggttttt
      601 gtttcccttt ttgatttcat cctgctgttt atattgttta ttccaatcaa ctattgctct
      661 taacgctatc aaagtaaaga cggcaaaaag cagcgctgta aaaaacaacc ccatataagc
      721 ataaagggct gtgctcaaca cgtcgacaac tatccagata atccagttat cctttacttt
      781 tcttgaaagc aaaaactgcc ccattacact agccgctgtc aacatggcat ccaacctggg
      841 aaaggctgga ggatcaatgt attcgggaaa cctggactga aaataaataa ttccttcgct
      901 ccacaagccg ccgaacacca aaactatcac tatcaccaga gcccactggc tcttatttaa
      961 tcgccgtatg cgcagctctc tgtttttccc ttcttgtccg ggcgccgggt gcttccagtg
     1021 ataataccca tagaggctga atgagaaata cactacctgc agcagcatgg ctgagtataa
     1081 atgtgcctga taaaacacaa caaagtacac cacattgttg agcaaaccaa acaaaaaatt
     1141 aacagagagc gctcttgagg ccaaatatac agccaccagc ccgctaacca atccgatcaa
     1201 ttccagcagg ttcatggaat ggccccataa cgaaaacaac actacatcct gaatcgacat
     1261 attatccaac actcccttcc agagaaacct gcaacaattt ttgtgcctca acggcaaatt
     1321 ccatgggaag cttattgatt acttctttag catagccatt cactatcaac cctatagctt
     1381 cttctgtaga aataccgcgc tgattgcaat aaaacaattg atcctcgttg atcttagaag
     1441 tagtcgcttc gtgctcaact acggctgttt cgttgtttat ttcaatataa gggaaggtat
     1501 gtgccccaca ggtgtccccc agcaacaaac tgtcgcattg ggagtgattg cgggcgttgg
     1561 aagctttagg agcaattttt accaacccac ggtaactgtt ttggctttta ccggccgata
     1621 tacctttgga aacaataatg ctgcgggtgt ttttgcccaa atgaatcatt ttggttcccg
     1681 tatcggcctg ttgatgattg ttggtaacgg caaccgagta aaattctccc acagaattat
     1741 cccccgccaa aatacaggaa gggtacttcc aggttatggc tgagcccgtt tcaacctgag
     1801 tccaggagat cttggccgat tcacctttac acaaacaacg cttggtaaca aagttataaa
     1861 taccaccctt gccttcttta tcgcccggat accagttttg tacagtagaa tatttgactt
     1921 ctgcccggtc gtggacaaca atctccacta tagccacatg caattggttc tcgtcgcgta
     1981 tcggagccgt gcagccttcc aggtagctga cgtagctatc gtcatcggcc actatcaatg
     2041 tgcgctcaaa ttgacccgta ttgatggcat taattctgaa ataggttgac aactccatag
     2101 gacaacgcac ccccttaggt atgtacacaa aggaaccatc gctgaaaacg gcacaattca
     2161 gggccgcgta ataattatcc gtatagggta cgaccgagcc caggtaacgc ttgattaatt
     2221 cgggatgctc ttgcaccccc tcgctgaaag agcaaaaaat gatgcccttc tttgccaggc
     2281 tttccttgaa ggtggtcttg acggaaacac tatccattac ggcatctacg gccatgcctg
     2341 ccagttgctt gcgctcttgc aagggtatgc ccagtttctc gaaggttttt agcaaatccg
     2401 gatcaatgtc ttcctcgtta tctccggaag ttttgctctt aggggcggca tagtagacca
     2461 ttttttgata gtcaatttcg ggaatgtcca aatgcgccca ttggggcttt tccatgcttt
     2521 gccaatgacg aaaagccttg aggcgaaact ccaacagcca gtccggctcc atttttttgg
     2581 aagaaatcaa acgaataaca tcttcattta acccctcggg aatagtttcg gtttcaatat
     2641 cggtcacgaa accatactta tattcaccgg cagttacctg tgtaatgata tccttgtctt
     2701 cggttgattt catacatcat atactctgag ggacaggtgt tgattgttgt aaagtttcgg
     2761 gtttttttgt gttccagtct tcaaagtgga taaagggcag cacggcggga gcaaaatcgg
     2821 aaagcgggcc gtaaaaacga gaagtactgc ttttttcccg atgaaaagaa gacttccctt
     2881 tgtcaaaata gtcaaacaca ttaacgacta tactcaacac aaaaaggtaa agcaccacgg
     2941 caaataaacc tccggcaatg cggttgagcc agccgagcat caccattttg accagcttgc
     3001 ccagtaaaac cgctacaaaa tgaaacaaga tgaccgtcag cacaaaaagt actataaacg
     3061 aaacaacctg caccactttt aggctcgccg actcaaaaag ttgggaaatg taaggagcca
     3121 ccaaatacga gagtgctttg gctgcaaaga ttccacaaac aacgcctgct atagttccaa
     3181 tggtctgtat cagacctctg cgcaaaccat caattaagcc taacaaagca acgatcaaaa
     3241 taatgatgtc gtagagattc atacgctaca gggtctgttt tacttctatt tcttcgtagg
     3301 cttcgattaa atcgcccacc ttgatgtcgt tataattgtt aatactcaat ccacactcat
     3361 aacccgaggc aacttccttg acatcctcct tgaagcgctt taatgagctc aattctccgg
     3421 taaatacaac tataccgtca cgaataagcc taattttagt acctcgcttg atttttcctt
     3481 ctttaaccat acagccggca actgtaccga ccttagtaat cctaaatact tcacgaacct
     3541 cggctgtggc agtaacttcc tccttgattt cgggagaaag cataccttcc atagccgact
     3601 taatttcttc gatggcatcg tagataattg aataaagacg aatgtccacg ccatcgcgct
     3661 cggccatctt gcgggcggcc atagaggggc gtacctgaaa accgacaata atggcatcgg
     3721 aagcagaagc cagtgttaca tccgattcgg aaatctgacc gaccgccttg tgtatcacat
     3781 tcacctgaat ttgctctgtg ctcaaaccta tcagagaatc cgacaaagct tcaataga
//
LOCUS       DAOSTD010000122         3815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_21562_length_3815_cov_10.683777, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000122 DAOSTD010000000
VERSION     DAOSTD010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21562_length_3815_cov_10.683777"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..964
                     /locus_tag="PLI37_07175"
     CDS             323..964
                     /locus_tag="PLI37_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58826.1"
                     /translation="MIRRLFIPAVVFFSINYSCFAGENGAKFGYRGFSGGMMPHSGYF
                     WGGKTTVRDARGTVLDEEEMRGIPLGLGGAIRFHFGDHFRIGAEGYGTTLRYGNYNSN
                     VSIGWGGLLAAYYCEYGKIKPFAGATLGGGSVKNLTLLQDNPLDDIAEPAVSYRNYPF
                     MAFCPFAGAEYCLNKKIHLIFKVDYLMNISNPQDDFVRGPRIYFGFVFYHIKR"
     gene            970..1680
                     /gene="ubiE"
                     /locus_tag="PLI37_07180"
     CDS             970..1680
                     /gene="ubiE"
                     /locus_tag="PLI37_07180"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOQ58827.1"
                     /translation="MPKKKAVKHLFDRIAPHYDRLNHLLSLHVDKTWRRKAVQKVLEV
                     PHERVMDMACGTADFSLALAKAGVPSVLGLDISEQMLRIGRQKIQSQGMSRIIQLERG
                     DSEAIAYPDKSFDVVTVAFGVRNYENIELGLQEMLRVLKPGGMLLVLELSLPQSRFLY
                     ALYSFYFTRILPWVGGLISGDKQAYAYLPASVLRFPEPGEFLQIMQSCGCVETRQQAL
                     SLGLARIFTGKKQITGKK"
     gene            complement(1664..2560)
                     /gene="menA"
                     /locus_tag="PLI37_07185"
     CDS             complement(1664..2560)
                     /gene="menA"
                     /locus_tag="PLI37_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HOQ58828.1"
                     /translation="MKSIKLYIKTFRLRTLPLSVSGIVLGSLLAASEGYFSVLTFILA
                     ILTTLCLQILSNVSNELGDAQKGTDTADRKGPAYVLLSGALTQKDFYRLIVMFSLLSV
                     LFGSLLVYVSLDYFFSKEGLIMLAFGGLAILASITYTLGKNPYGYKGWGDLFVFLFFG
                     LLSTLGSYFLQCGQLPLLLLLPASACGFLITGVLNLNNIRDMETDKINRITLPLLLGK
                     PKAKIYHHLLIYGAFVLILIYNLLKGTAWPQYLFLLLLPLAVFHLSRVRALNDGALDR
                     QLPVLVLLTILFSLVVGFTSFL"
     gene            complement(2607..3431)
                     /locus_tag="PLI37_07190"
     CDS             complement(2607..3431)
                     /locus_tag="PLI37_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOQ58829.1"
                     /translation="MTSSQTKPYAIGLALSGGGAKGFAHAGALKALEEVGIYPDIISG
                     TSAGAIVGSLYADGCTPHEIIELFNNTSFYKFASFARSKSGLFKIDNFRKAMNQKLKA
                     KTFEELSIPLYVNATDMFHGKNVFFSSGSLLDKVIASCSIPIIFEPVLIDGIHYSDGG
                     LLCNFPVEVLRKKCKYLIGVNVGPVDIDHEEQLNLLDIIQRTYFFIRRASVLNVREQC
                     DLLIEPPSIDKYGMFDAVKNEEIFQLGYQAALAILNEQKEKVKEMQALSFARQTTR"
     gene            complement(3526..>3815)
                     /locus_tag="PLI37_07195"
     CDS             complement(3526..>3815)
                     /locus_tag="PLI37_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOQ58830.1"
                     /translation="KIEIFEFDPNIRKFEDLQEGMVLPGIVTNITNFGCFVDIGIKEN
                     GLLHLSQMADRFVAHPTEVLSLHQHIKVKILSLDPERRRIQLSLKNNPDAS"
BASE COUNT         1020 a          826 c          954 g         1015 t
ORIGIN      
        1 cgacaactgt agtgtcaact ttagaaccgc ctttttgctg acaagagatc agaaagagag
       61 cggcaaacaa aagaaccaaa gcaattgatt ttttcattgg agtaattaat aaaaaataag
      121 taaaaaagga tgttgaaaac cgccgcgaag atatacaata tatcctgaaa ttgttctttt
      181 ttgtaaaaaa aatttttgta gaagaaaata ttttttacaa gccgttctgc agcttgcttt
      241 tttagtcttt ccgggaatgc ttaactttgc gctttttcaa aagagaccgg cttttagggt
      301 ttaaaatccg gcaattgata caatgattcg aagattattt ataccggctg ttgttttctt
      361 tagtattaac tacagttgct ttgccggcga aaacggagca aaattcggat accggggttt
      421 ctccggtggt atgatgcccc acagcgggta tttttggggc ggaaagacaa ccgttcgtga
      481 tgcccgggga acagttctcg atgaggaaga aatgagagga atacccctgg gcttgggcgg
      541 agccattcgc tttcatttcg gagatcattt tcgcatcggc gccgagggat acggcactac
      601 actccgttac ggaaattaca acagcaacgt ctccataggc tggggaggtc ttttggctgc
      661 ttattattgc gaatacggta aaatcaaacc ttttgccgga gccaccctgg gaggcggttc
      721 ggtcaaaaat ctgactctat tgcaggacaa tcctctcgat gatatagcgg aaccggccgt
      781 atcctacaga aactatcctt ttatggcttt ctgtccgttt gcaggagcgg aatactgtct
      841 gaacaaaaaa atacatctga tcttcaaagt cgattacctg atgaatattt ccaaccctca
      901 ggatgatttt gtgcgaggcc ccagaatcta tttcggtttt gtgttctatc acatcaaacg
      961 ctaagtttca tgcccaaaaa gaaagccgtt aagcatttat tcgaccggat tgccccgcat
     1021 tacgaccgct tgaatcattt gttgtcgctc catgtggata agacctggag acgtaaggct
     1081 gtccaaaaag ttcttgaggt tcctcacgaa cgggtaatgg atatggcttg cggaacggcc
     1141 gatttcagcc tcgctctggc caaggccggt gttccctcgg tgttagggct cgatatttcg
     1201 gaacaaatgc tgcgtatcgg ccggcaaaag attcagtctc agggcatgag ccgtatcatt
     1261 caattagaac ggggcgacag cgaagccatt gcttatcccg acaagagttt cgatgtcgta
     1321 accgtggctt tcggtgtacg caattacgaa aacatagagc tgggtttgca agaaatgctg
     1381 agggtgctta aaccgggagg aatgcttttg gttttggaac tttctctgcc tcagagtcgt
     1441 tttttgtatg ctctctattc tttttacttc acccgtattt taccttgggt aggcgggctg
     1501 atttcgggcg ataagcaagc ctatgcctat ttgccggctt ccgttctgcg ttttcccgaa
     1561 cccggggaat tcctacaaat tatgcaatcc tgcggctgtg tcgaaacccg gcagcaggct
     1621 ctgagcctgg gactggcccg tatttttaca ggaaagaagc agattacagg aaagaagtaa
     1681 atcccaccac cagactaaac aggatggtca gcagcaccag aaccggcagt tgtcgatcga
     1741 gcgccccgtc gttgagcgca cgtacacgac tcaggtgaaa taccgccaag ggaagcaaga
     1801 gtaaaaaaag atattgaggc caggctgtgc ctttaagcag attgtaaatc aggatcagca
     1861 caaaggctcc gtatatcagc agatggtgat agattttggc cttaggtttg cccaacaaca
     1921 ggggcagcgt gatgcggttg atcttgtcgg tctccatgtc ccggatattg ttcaggttga
     1981 gtactccggt gataagaaaa ccacaggccg aggcgggaag caagagcaaa agaggcagtt
     2041 gtccgcactg caaaaaataa gatcccaggg tactcagcaa gccgaaaaac agaaagacga
     2101 acaaatcgcc ccagcctttg tagccgtagg gatttttgcc taaagtgtaa gtgatggaag
     2161 caagaatggc cagacctcca aaagcgagca tgatcaggcc ttctttgctg aagaaataat
     2221 ccagcgacac gtacaccagc aaactgccga agagcacgct gagcaggctg aacataacaa
     2281 tcagccggta gaaatccttt tgggtgagag ctcccgaaag cagcacataa gccgggcctt
     2341 tgcgatcggc ggtgtcggtg cctttttggg cgtcgcccaa ttcgttggat acgttcgaca
     2401 gaatttgcag acacagcgta gttaggatgg ccagaataaa ggtcaggacg ctgaaatacc
     2461 cttcggaagc cgccagcaaa ctgcccagga ctatgcccga aaccgacagg ggcagggttc
     2521 tgaggcggaa agttttaata tagagtttaa ttgatttcat gtttcatttg tagtgaacgc
     2581 ctgagctgat ggcggtttgg gcgaatttac cgtgttgttt ggcgggcaaa gctgagagcc
     2641 tgcatttctt ttactttttc tttttgttca ttcaggatgg ccagggctgc ctgataaccc
     2701 aattgaaaaa tttcttcgtt tttgacggca tcgaacatac catacttatc gatcgaaggc
     2761 ggttcaatca agaggtcgca ttgttctctc acgtttaaaa cgctggcccg gcgaatgaaa
     2821 aaataagtgc gttgaataat gtcgaggagg ttgagctgct cttcgtgatc tatatctacc
     2881 gggcctacgt tgacaccaat caggtatttg cattttttgc gcagtacctc aaccggaaag
     2941 ttgcacagga ggcctccgtc ggaataatga attccatcga tcagtaccgg ttcgaaaata
     3001 atgggaatgc tgcaggaagc tataaccttg tccaataaac tgccggagct gaaaaacacg
     3061 tttttgccgt gaaacatatc cgttgcgttc acgtacagag gaatgctcag ttcttcaaaa
     3121 gtcttggctt tgagtttttg attcatagct ttgcggaagt tgtctatttt aaacagaccg
     3181 cttttggaac gggcaaagct ggcgaattta taaaaagagg tgttgttgaa aagttcaatg
     3241 atttcgtggg gagtgcagcc gtcggcatac aaagatccga cgatggctcc ggcgctggtg
     3301 cccgaaatga tgtcgggata aataccaact tcttccaggg ctttgagcgc accggcatgg
     3361 gcaaaaccct tggctcctcc gccgctcagg gccagaccga tagcatatgg tttcgtttgt
     3421 gaggatgtca tatagtcaaa acttggcaat acaaaagccc ggcaaaatta aggtattttg
     3481 tctaatgagg caattttgtt tttttctcag gaagcttcgt ttttctcagg aagcatcggg
     3541 attgtttttt aagcttaatt gaatacgcct gcgttccgga tcgagcgata gaattttcac
     3601 tttgatgtgt tgatgcagac tcagcacctc ggtggggtgc gccacaaagc ggtcggccat
     3661 ctgagaaaga tggagtaaac cgttttcttt aatgccgata tccacgaaac aaccaaaatt
     3721 ggtgatgttg gttactatgc cgggcaaaac cattccctct tgcagatcct cgaattttct
     3781 gatgttggga tcgaattcaa agatttcgat ttttg
//
LOCUS       DAOSTD010000123         3772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_21871_length_3772_cov_14.260963, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000123 DAOSTD010000000
VERSION     DAOSTD010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21871_length_3772_cov_14.260963"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLI37_07200"
     CDS             <1..231
                     /locus_tag="PLI37_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOQ58831.1"
                     /translation="PVMNSEGIRDVYLPEGEWVHFFSRKKYSGGRWIKAMEVPLDEMP
                     VFVKAGSSIPLYPEKVSCTDEMDMDKVIREHF"
     gene            242..1306
                     /locus_tag="PLI37_07205"
     CDS             242..1306
                     /locus_tag="PLI37_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58832.1"
                     /translation="MYNWKSNWETSKKHYMDWWQHQGIVLTMWEHIDKEGAPWEQVPV
                     PAPAKDLNQFWFDPEWRSGYLHHKMAGYSFMADILPVANTQLGPGSLGAILGAELEGR
                     EDTIWIRDQAGFDGNIVLDPNNRWWQLHRDLLKKCRQKSQDKYFVGMPDLVEGLDVLS
                     SLKGPDNVLMDLILDPEGSLQQLKAINTAWFEVFDRLYDIIHVNGEMAFCYFSIWGPG
                     KVAKLQCDISVMISEEDFRTFSLPFLKEQCEYLDYSLYHLDGVDAIRHLDAVLELKQL
                     NAVQWTPGVGQPQGGDPRWYDLYKKILDGGKSVMINWVQLNELEALLDTLGNRGLNIQ
                     MDFKSEKDIEQALNIVEKYR"
     gene            1309..2868
                     /locus_tag="PLI37_07210"
     CDS             1309..2868
                     /locus_tag="PLI37_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478662.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOQ58833.1"
                     /translation="MRTLVELSTLDIIIFVAYLVILGLVAAWASFGNRKKEGNLFLAN
                     KSLGWFAIGLTMWGTNVGPSMLIANASSGFEGGIAAGNFSWYAFPFIMLLAFVFAPRY
                     LGAKVTTLPEYMGKRFGEQTRNYIAWYSILTILISWLGLTLFSGGVFMAQLLAIPLWA
                     CIAILVAVSALFTMAGGLKAIAYTNVYQMLLLIGVSVLLVVMGINKVGGLGSVVRDTP
                     VEFWNLFRPLDDKDFPWLAILVGYPVMGVWFWCTDQSMVQSVLGAKNLKEGQKGANFC
                     AWLKLLDIPLFILPGILCFLLLPGLANSTDSYLQLVNAVFPSGLMGLVIVVMVAALIS
                     TIGSALNSLSTVFTMDIYIKKFRPDAGDSNIRTIGRWVVLIGALISVFLAIGITYIQG
                     LSFFNIFQSVLGFLAPPMAASFLFAAFWKKTSTQAINLVLTVGTAFSIGTGILYYTGL
                     IFSGMHYLYLSFFIFVVLALFIFIWSMATSKPSLQTMEYVPIKVSKGVKLAWGILVLC
                     MIGVYLFFNGF"
     gene            2880..>3772
                     /locus_tag="PLI37_07215"
     CDS             2880..>3772
                     /locus_tag="PLI37_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOQ58834.1"
                     /translation="MNWVDELLQVKTVKALPIMTHPGIEKIGKTVRDAVTDGRVHYEA
                     IRYLSDHYPSMACTVIMDLTVEAEAFGSKITMPEDEVPSVTDRLVQDREDVEALEIPG
                     MDAGRLPEYLLANRLAVENIKDKIVLSGCIGPFSLAGRLFGLSEIMMEIYMDPDTIHL
                     LLDKCTRFLISYCKALKTIGTAGVFMAEPAAGLLSNEDCQTFSSDYVRQIVEALQDDQ
                     FNIILHNCGNSGQCTQAMIDSGARVLHFGNLTDMEEALRVCPPDVLVMGNLDPVGLFK
                     LGSPQKVRESSRQLLDKAKSYR"
BASE COUNT          938 a          785 c          967 g         1082 t
ORIGIN      
        1 cccgttatga attccgaagg tattcgcgac gtgtatttgc ccgaagggga atgggtacat
       61 ttcttcagtc gcaaaaaata cagcggaggc cggtggatca aggctatgga agtgcctttg
      121 gacgagatgc cggtttttgt taaagccggc agcagtatcc ctctttatcc cgaaaaggta
      181 tcctgcacgg acgaaatgga tatggacaaa gtaatcaggg aacattttta aacacaaaaa
      241 catgtacaac tggaaatcaa attgggaaac cagtaaaaaa cactatatgg attggtggca
      301 acaccaaggt atagtgttga ctatgtggga acatatcgac aaagaaggag caccctggga
      361 acaggtgccg gtgcctgctc ccgccaaaga cctgaaccag ttttggttcg atcccgaatg
      421 gcgctccggc tatttgcatc acaaaatggc gggttattcg tttatggccg atattttacc
      481 cgtagccaac acgcaactgg ggcccggatc gctgggagcc atattgggtg cagagctcga
      541 aggccgggaa gataccatct ggataagaga tcaggccgga ttcgacggaa acatcgtttt
      601 ggatccaaac aatcgctggt ggcaattgca ccgggatctg ttgaaaaaat gccgtcaaaa
      661 atcgcaggat aagtattttg tgggtatgcc cgacctagtt gagggtctgg acgttttatc
      721 cagcctcaaa ggccccgaca atgtgttgat ggatctgata ttggaccccg aaggtagtct
      781 gcagcaactc aaggccatca atacagcctg gtttgaggtg ttcgaccgtt tatacgacat
      841 tatccatgtg aacggagaaa tggccttttg ttatttttcc atctggggcc ccggcaaagt
      901 tgccaagttg caatgcgata tttcggtcat gatttccgaa gaagatttca ggactttctc
      961 cctgcctttt ctgaaggaac aatgtgagta cctggattac agtttgtatc acctggacgg
     1021 tgtggatgcc atccgtcatc tggacgcagt gctcgaacta aaacaactga acgccgtaca
     1081 atggacaccc ggcgtgggtc agcctcaggg aggagatccg cgttggtatg atttatacaa
     1141 aaaaattctg gatggaggta agtcggttat gatcaattgg gtacagctaa atgaactgga
     1201 agctttgctc gatactctcg gcaaccgggg attgaatatt cagatggatt ttaagtcgga
     1261 aaaagacata gaacaagcct taaacatagt tgaaaaatac agatagaaat gagaaccctt
     1321 gttgaattgt ccactctgga cataataatc tttgttgctt atttggtaat attgggcttg
     1381 gtggcagcct gggccagttt cggaaacaga aaaaaggaag gaaatttatt cctggccaat
     1441 aaaagtctgg gctggtttgc tattggtttg accatgtggg gcaccaatgt aggcccctcg
     1501 atgttgattg ccaatgccag cagcggtttt gaaggcggca ttgcggcagg aaatttttcc
     1561 tggtatgctt tcccctttat catgctcctc gcttttgtgt ttgcgccccg ctatttggga
     1621 gcaaaagtca ccactctccc cgaatacatg ggcaaacgtt ttggagaaca aacacgcaac
     1681 tacattgcct ggtattctat cctgactatt ttgatatcct ggttgggtct gactctgttt
     1741 tcgggcgggg tttttatggc tcaattattg gctattcccc tttgggcctg catagcgatt
     1801 ctggtggctg tgtctgcttt gtttaccatg gccggaggct tgaaggccat agcctatacc
     1861 aatgtttatc agatgctttt actgatcggg gtttcggtgt tgctggtggt aatgggaatt
     1921 aataaagtcg gtgggttggg ttcggtggtc agggatactc cggtggaatt ttggaatctt
     1981 ttccgtcctc tcgacgacaa ggattttccc tggctggcca tattggtagg ttatcctgtt
     2041 atgggagtct ggttttggtg taccgatcaa tcgatggtgc aatcggtatt aggagccaaa
     2101 aacctgaaag aaggccaaaa aggggcgaat ttttgcgcct ggctcaagtt acttgatata
     2161 cctttgttca tcctaccggg tattttgtgt ttcctgcttt tacccggtct ggctaattct
     2221 accgattcct acttgcaatt ggttaatgca gttttccctt ccggcttgat gggactggtt
     2281 attgtggtta tggtagcggc tctgattagt actatcggat cggcattgaa ttccctaagc
     2341 acggttttta ctatggatat ttacatcaag aaattccgtc cggacgccgg cgacagcaat
     2401 attcgcacta taggacgctg ggtggtttta attggagcac tgatttcggt atttttggcc
     2461 atcggtatca cttatattca gggtttgagt ttctttaata ttttccagtc ggtgttgggc
     2521 tttttggctc ctcccatggc tgccagtttc ctttttgcgg ccttctggaa gaaaacttcg
     2581 actcaggcca ttaacctggt gctcacagtc ggaacagctt tcagtatcgg aaccggaata
     2641 ttgtattata caggattgat atttagcgga atgcattatt tatatctttc cttctttatt
     2701 tttgtggttc tggctctttt tatttttatc tggtcaatgg ccacatctaa acctagttta
     2761 cagacaatgg aatatgtgcc cattaaagta agtaagggag taaaactagc ctggggtatc
     2821 cttgtgttgt gtatgatagg agtgtatttg tttttcaatg gtttttaaag ttagaaatta
     2881 tgaattgggt agatgaattg ctgcaggtga aaaccgtcaa ggccttgccg ataatgaccc
     2941 atccgggcat agaaaaaata ggtaagacgg tgcgggacgc tgtaaccgat ggcagagtgc
     3001 attacgaagc cattcgctat ttatcggacc attatccctc tatggcttgc acggtcatta
     3061 tggatttgac cgtcgaagcc gaagcattcg gatcaaaaat aaccatgccc gaagacgaag
     3121 tgccttccgt aaccgaccgt ctggtacaag atagggaaga tgtcgaggct ttggaaattc
     3181 ccggcatgga tgccggcagg ctgcctgaat atttattggc caaccggctg gccgtcgaaa
     3241 acatcaaaga caagattgtt ttgtcgggat gtatagggcc cttttcgctg gctggccggc
     3301 tgttcggtct ttccgaaatc atgatggaaa tttacatgga tcccgatacg attcatctct
     3361 tgctcgataa atgcacccgt tttttgatca gttattgtaa ggcccttaaa accattggaa
     3421 ctgccggggt ctttatggcc gaaccggctg cgggattgtt gtccaacgaa gactgtcaaa
     3481 cattttcgag cgattatgtt cgtcaaatag tagaagctct gcaagacgat cagttcaata
     3541 ttattctgca taattgcggt aacagcgggc aatgcaccca agccatgata gacagcggag
     3601 ccagggtctt gcatttcgga aacctcaccg atatggaaga ggctctcagg gtttgtcctc
     3661 cggatgtttt ggtgatgggc aacctcgatc ccgttgggtt gttcaagttg ggcagtcccc
     3721 aaaaggtcag ggagagcagc cggcaattgc tcgacaaagc caaatcatac cg
//
LOCUS       DAOSTD010000124         3652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_22825_length_3652_cov_13.079789, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000124 DAOSTD010000000
VERSION     DAOSTD010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22825_length_3652_cov_13.079789"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PLI37_07220"
     CDS             <1..956
                     /locus_tag="PLI37_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOQ58835.1"
                     /translation="MEGPAVEAFFAAEQPEYVVLAAAHVGGIQANNVYRADFIYRNLQ
                     IQQNIIGAAYRHKVKKLLFLGSTCIYPRDAAQPIREEALLTGPLEYTNEPYAIAKIAG
                     LKLCESFNLQYGTNFIAVMPTNLYGPNDNFDLERSHVLPAMVRKIFLADSLLRGDETL
                     LRRDLDSRPVEGLDGKAPLPEIKVLLQKYGIYPDRLELWGTGKPLREFLWSEDMADAC
                     VFIMERVDFEDLRPKDLSAEVRNCHINIGTGKEISIAALAAMIAEKTGYTGKISFDAS
                     KPDGTMRKLCDVNKLHKLGWQHRVDISEGLDRILDWYRRSL"
     gene            1022..>1770
                     /locus_tag="PLI37_07225"
     CDS             1022..>1770
                     /locus_tag="PLI37_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ58836.1"
                     /translation="MMFNPQKYAIPDRRMQPFIDPDEIRACLQNARPDARAVRDTIAR
                     SLSRERLSMQETALLLNAEDPELIEEIKEGARQLKREIYGNRIVLFAPLYIGNKCIND
                     CVYCGFRASNREAVRKTLSDEEIVQEIASLEDQGQKRLILVYGEHPDYDAHYIAHTVK
                     LAYGVKKDRGEIRRVNINAAPLDIEGFRVVKAAGIGTYQIFQETYDPQAYSLYHPRGH
                     KADYAYRLTALDRAQEAGIDDVGIGALFGLY"
     assembly_gap    1771..1870
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1871..2234
                     /locus_tag="PLI37_07230"
     CDS             <1871..2234
                     /locus_tag="PLI37_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="in Escherichia coli this enzyme functions in
                     thiamine biosynthesis along with thiFSGI and IscS; with
                     ThiFSG catalyzes the formation of thiazole phosphate from
                     tyrosine, cysteine and 1-deoxy-D-xylulose-5-phosphate;
                     forms a complex with ThiG; contains an iron-sulfur center;
                     in Thermotoga this enzyme has an extra C-terminal domain;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOQ58837.1"
                     /translation="HKDLRRGQFLINDDRPLNDIIDELISMDMIPSFCTSCYRMGRTG
                     EHFMEFSVPGFIKRYCTPNAILTLAEYLLDYAPEHTSRKGWELIEREMARMDEGPVKK
                     KLEQKLELLKSGQRDCYF"
     gene            2251..3459
                     /gene="hydF"
                     /locus_tag="PLI37_07235"
     CDS             2251..3459
                     /gene="hydF"
                     /locus_tag="PLI37_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOQ58838.1"
                     /translation="MAIIPRNDLKPHIGIFGRMNVGKSSVINALTGRKQAIVSAVPGT
                     TTDPVRKAMELGGLGPCVLIDTAGTDDSTELGRQRVDKSLEVLSEMDLALILFSENKL
                     EEPELLLLERCRHLGIPCLLLHNKSDVQALNRAVLPAIEASIPVLEFSALYPEKYLPA
                     LLKAMGQLLPEASAQPDSLFKGIVSRGDMVVLVTPIDRGAPKGRLILPQVMAIRDLLD
                     NEAFCVVLKETQLEDYLNSSGARPALVITDSSVFDQVNKLLPPHIPLSSFSIAFARWR
                     GPFEDYIKGTPYLDKLQDGDRVLIREACTHLPSCQDIGRVKLPAWIRRYTGKQLEIEL
                     CPANNKALHPMASYAMVIQCGACMSTPRQVRAGLAEAMAAGVPLSNYGLAIAWMNGIF
                     DRVISPFVKL"
     gene            3471..>3652
                     /locus_tag="PLI37_07240"
     CDS             3471..>3652
                     /locus_tag="PLI37_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58839.1"
                     /translation="MDTPVSFFTNHSPSEEELAYWLEPGSVEKDRQLFDRAYAAKIEH
                     PGPRLHLRFLLEVSNV"
BASE COUNT          857 a          892 c          952 g          851 t
ORIGIN      
        1 tgatggaagg ccctgcggtg gaggcttttt ttgcagccga acagccggaa tacgtggtgt
       61 tggcggccgc tcatgtgggc ggcattcagg ccaacaatgt gtacagggcc gacttcatct
      121 accgcaacct gcaaatccag caaaacatca taggagcggc ctatcgtcat aaggtcaaaa
      181 aattgttgtt tttgggcagt acctgcatat atccccgcga tgcggcccag cccatccgcg
      241 aggaagcctt gttgaccggt cctctggaat acaccaacga accctatgcc attgccaaga
      301 ttgccggact taagctttgc gaaagtttca acctgcaata cggcaccaat tttattgcgg
      361 tgatgcctac caatctctac ggccctaacg ataattttga cctggaacgc agccatgtgt
      421 tgccggctat ggtccgcaag atttttttgg cagatagcct gctcaggggc gacgaaaccc
      481 ttttgcgccg cgatctggac agccgtccgg tagaaggcct cgatggcaag gctcctttgc
      541 cagagattaa ggttttgctg caaaagtacg gtatttaccc cgatcgcctc gagctttggg
      601 gcacgggaaa acccttgagg gaattcctct ggagcgaaga catggccgat gcctgtgtct
      661 ttattatgga aagggtcgat ttcgaagacc tgcgacccaa agatctctcg gccgaagtgc
      721 gcaattgcca catcaacata ggtacgggca aagaaatcag tatagccgct ctggctgcta
      781 tgattgcaga aaaaacaggc tataccggca agataagttt cgatgcttcc aaacccgacg
      841 gcaccatgcg taaactttgc gatgtgaaca agttgcataa actcggctgg caacaccggg
      901 tggatatttc cgaaggtttg gacagaatac tcgactggta ccgccgcagc ctgtagattt
      961 aattcataaa aaaaagtcgt tttcggtttt ggagttgtat ctttgatacc ccaaatgcct
     1021 aatgatgttt aatccccaaa aatatgccat tccggatcga aggatgcagc cttttatcga
     1081 tccggacgag atacgtgctt gcctgcaaaa tgccagaccc gacgcccggg cggtacgcga
     1141 cacgatagca cgctcgcttt cccgcgaacg cctcagtatg caggaaaccg ccttgctgtt
     1201 aaacgccgaa gatcccgagt tgatcgaaga aatcaaagag ggtgcccggc aactcaaacg
     1261 cgaaatttac ggtaatcgaa tcgtattgtt tgcccctctg tatatcggca ataaatgtat
     1321 aaacgattgc gtgtattgcg gtttcagggc ttctaatcgc gaggctgtgc gtaaaaccct
     1381 gagcgacgaa gaaatcgttc aggaaatagc aagcctcgaa gatcagggac aaaagcgcct
     1441 gattttggtc tatggcgagc accccgatta cgatgcgcat tacatagccc ataccgtgaa
     1501 gttggcttac ggcgtaaaaa aagaccgggg cgagatcagg cgggtcaata tcaatgccgc
     1561 tcccttggac atagagggtt ttcgtgtggt aaaagcagcc ggcataggca cttatcagat
     1621 ttttcaggaa acctacgatc ctcaggccta ttctttgtat catccccgtg ggcacaaggc
     1681 cgattacgct tatcgcctca cggccctgga ccgtgcgcag gaggccggca tagacgatgt
     1741 gggcataggt gcgctctttg ggctttacga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn gcataaagac ctgcgccggg gtcagttcct tataaacgac gaccgcccgc
     1921 tcaacgatat tatcgatgaa ttgataagca tggatatgat tccttctttt tgtacctcct
     1981 gttaccgtat gggcagaacg ggtgaacatt ttatggagtt ttcggtgccg ggctttatca
     2041 agcgctattg cacccccaat gccatactta ccctggctga gtacctgttg gattacgcgc
     2101 ccgaacacac ttcccgcaaa ggctgggagc tgatagagcg ggagatggcc cggatggacg
     2161 aaggtcccgt aaaaaagaag ctggaacaaa aattggagct gttgaagtcg ggacaacgcg
     2221 attgttattt ttaagttcaa agctctggat atggctatta tccccagaaa cgacctgaaa
     2281 cctcatatcg gaatctttgg ccggatgaat gtgggcaaaa gttccgttat caatgccctg
     2341 accggccgca aacaagccat agtgtcggcc gtgcccggaa ccaccaccga cccggtgcgt
     2401 aaagccatgg aattgggcgg gctggggccc tgtgtgctta tagacacggc cggtacggac
     2461 gattcgacgg agttgggccg gcaaagggtg gacaaaagcc tcgaagtctt gtcggaaatg
     2521 gatcttgctt tgattctttt cagcgaaaac aagctggaag aacctgaact cctgttgctg
     2581 gagcgttgcc gccatctggg aattccctgt ttgctgctgc acaacaaatc cgatgtgcaa
     2641 gctctgaacc gggctgtact gcccgccatc gaggctagta ttccggtgtt ggaattcagt
     2701 gccttgtatc cggaaaaata cctgccggcg ttgttgaagg cgatggggca attgcttccc
     2761 gaagcctcag ctcagccgga ttcgctgttt aaaggcatag tgagcagggg cgatatggtg
     2821 gtgttggtaa cacccatcga ccggggagcg cccaaagggc ggctgatcct gccccaggtg
     2881 atggccatac gcgatttgtt ggacaacgag gctttttgtg tggtgctcaa agaaactcag
     2941 cttgaggact atttgaacag tagtggtgca aggcctgccc tggttattac cgacagcagt
     3001 gtcttcgatc aggtaaataa actattgccg ccccatattc ccctgagcag tttcagcatt
     3061 gcctttgcgc gatggcgagg tcccttcgaa gattatatta agggaacccc ctacctggat
     3121 aaactacagg acggagatcg cgtgcttatt cgggaggcct gcactcattt acccagttgc
     3181 caggacatag gccgggtgaa gctgcccgcc tggatacgcc gctacacggg caaacaactt
     3241 gaaatcgaat tgtgtccggc caacaacaaa gcccttcacc ccatggcctc ttacgctatg
     3301 gtgattcagt gcggagcatg tatgagtact ccccggcaag tcagggccgg tctggccgaa
     3361 gctatggctg ccggggttcc cctgagcaac tacggcctgg ccattgcctg gatgaacggt
     3421 atttttgatc gggtgatcag tccttttgtt aagctttgaa tctgcggttt atggatactc
     3481 cggtttcgtt ttttaccaat cattctcctt cggaagaaga attggcctat tggctggagc
     3541 cgggttctgt cgaaaaagac cggcaattgt tcgaccgggc ctatgctgcc aaaatcgagc
     3601 atccgggtcc ccgtttgcat ctgcgttttt tgctcgaagt gtcgaatgta tg
//
LOCUS       DAOSTD010000125         3570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_23531_length_3570_cov_17.622760, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000125 DAOSTD010000000
VERSION     DAOSTD010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23531_length_3570_cov_17.622760"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1734)
                     /locus_tag="PLI37_07245"
     CDS             complement(<1..1734)
                     /locus_tag="PLI37_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ58840.1"
                     /translation="MKDFIRLLFRLVPPYKKSVFLNVLNNFFSTLFSLFSFALVIPIL
                     EILFRINEQTYTYHENVWSLPTADIAGALKDNFYFFVSTTIEKSGGGATLLILGAFLI
                     AMTFLKTGFAYMASLNMITIRTSVLRDLRNTIFEKVLNLPLGFFSEEKKGDIISRMSN
                     DVTEVENSVMSSLDMTFKNFIMIIIYFTTLLIISWELTLFVLVLLPVAGFLMGRVSKQ
                     LKKPSRESQAQWGELLSQIEETLTGLRIIKAFNAENKIKSNFNRQNETLKRTTKRIIR
                     RQQLAHPMSEFLGTTIIAIVLWFGGSLILSESSHTITAPVFIYYLIIFYSIINPAKAF
                     SQAWYNVQKGLASMERIDRILLTENHIADPEHPVDIKGFTEAIEYKNVSFKYQTEPVL
                     ENIDLKISKGQTVALVGQSGSGKSTLVDLLPRFYDVNSGQILIDGIDIRQVRVKDLRA
                     LMGNVNQEAILFNDSFYNNIAFGVKNASIEQVVQAAKIANAHDFIIATEQGYDTNIGD
                     RGSKLSGGQRQRISIARAILKNPPILILDEATSALDTESEKLVQEALENLMKNRTTIV
                     IAHRLSTIRNAD"
     gene            complement(1966..3288)
                     /locus_tag="PLI37_07250"
     CDS             complement(1966..3288)
                     /locus_tag="PLI37_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOQ58841.1"
                     /translation="MAHQLKTYTLRNYKSIPQITNLSDEMIEAIEVVGRVLPFKTNNY
                     VIDELIDWTQIDTDPIFTLTFPRKEMLERRHYNAVKKLLDQGADQATLDKKVREIRME
                     LNPNPAGQEQNVPEINNIKLKGVQHKYSETVLFFPSQGQTCHAYCSFCFRWPQFSGMS
                     NLKFAMKETNLLAQYLLRNNKVTDVLFTGGDPMTMSTRVLASYIDVLLQPEFRNIHSI
                     RIGTKALSYWPYRFTKDKDSKELFKLFEQIIKAGKNLAVQAHFNHPAELKTDAVKKAI
                     KQIRATGAQIRTQSPLLKNINDKPEIWAEMWRKQVDLGCIPYYMFVARDTGSKRYFEL
                     PLEKCWNIFRKAYRQVSGLCRTVRGPSMSCEPGKIQVLGVQEVKGEKVYVLRFLQGRN
                     AKWVDIPFFAKYDPEATWYDQLEPAFGEEKFFFEKGRRRPKTNEILFE"
BASE COUNT          934 a          750 c          784 g         1102 t
ORIGIN      
        1 atcggcattc cggatggtcg acagccggtg tgcaatcaca atggttgtgc ggtttttcat
       61 caggttttcc agagcctcct gaaccaattt ttcggattcc gtatccaagg ccgaggtggc
      121 ttcgtccagt atcagaatag gcggattttt cagaatagca cgggctatgc tgatgcgttg
      181 acgttgaccg cccgataatt tactgccgcg gtctccgata ttggtgtcgt aaccctgttc
      241 ggtggctatg ataaaatcgt gggcattggc gattttagca gcttgtacca cttgttcgat
      301 gctggcgttt tttaccccaa aagcaatgtt gttgtaaaag ctgtcgttaa acagaatcgc
      361 ttcttggttg acattgccca ttagagctct gagatctttg actcttactt gccggatatc
      421 aattccgtcg atcagaatct ggccggagtt cacatcgtag aatcgaggaa gcaaatcaac
      481 cagtgtcgac tttcctgaac ccgattgccc gaccaaagcc acggtctgtc ctttggatat
      541 tttgagatcg atgttttcca acacgggttc ggtctggtat ttaaagctga cgtttttgta
      601 ttcgatggcc tcggtaaatc cttttatatc aacaggatgt tcgggatcgg caatatgatt
      661 ttccgtcaag agtatccgat cgattctttc catggaggcc agacccttct gtacattgta
      721 ccaggcttgc gaaaaggctt tggccggatt gatgatgctg taaaaaataa taaggtaata
      781 gatgaatacc ggtgctgtga tggtatgaga cgattcggat aaaatcagcg aaccaccaaa
      841 ccatagtacg atggcaataa tggttgtgcc cagaaattcg ctcatggggt gagccagttg
      901 ctgccggcgg ataatccttt tggttgtgcg tttcaaggtc tcgttttgcc ggttgaaatt
      961 ggacttgatt ttgttctctg cattaaaagc cttgattatt ctgaggccgg tcagtgtttc
     1021 ttctatttga ctaagtagtt ctccccattg tgcctggctt tcgcgcgagg gttttttcaa
     1081 ttgtttgctt accctaccca tcaggaagcc cgctacgggt aacaatacga gcacaaaaag
     1141 cgtaagctcc caactgataa tcaacagggt ggtaaaataa atgatgatca taatgaagtt
     1201 cttaaaggtc atatccagtg aactcattac ggaattctcc acttcggtca cgtcgttgga
     1261 catgcgcgaa atgatatcgc cttttttttc ttcggagaaa aaccccaggg gtaaattcag
     1321 aactttttcg aagatcgtat tgcgtaagtc cctcaagacg gaagttcgta tggtaatcat
     1381 attgagcgag gccatatacg caaaacctgt cttcaggaaa gtcatggcta tcagaaaggc
     1441 tcccaatatc aataaggtag cacctccccc gcttttttcg atggtggtgg aaacaaaaaa
     1501 gtaaaagtta tccttaagtg ctccggcaat atcggctgtg ggcaggctcc agacgttttc
     1561 gtggtaggtg taggtctgtt cgttgatccg gaataagatt tccagtatgg gaataaccag
     1621 ggcaaaggaa aacaggctga acaaagtcga aaagaaattg ttcagcacat tgaggaacac
     1681 actttttttg taaggcggga ccaaacgaaa cagcagtcgt ataaagtctt tcattatggg
     1741 gcagggtaga gataaacggc cgaaagatag tgcatataat ctggaaaaaa aagcctcgaa
     1801 aaatccttcg aggctttagg agagtttagc tctatccatg ctttcgacac cggctgcggt
     1861 tcttggacgc ccacgagcat acggcgtgtt gaaagccctc catgctttca accaccaggg
     1921 taacttttgg gtttagtttc taaatttctt gtcgttcttt tttcatcatt cgaataatat
     1981 ttcatttgtt ttcggtcttc gtcttccctt ttcaaaaaag aatttttctt ctccaaatgc
     2041 gggttctaat tggtcatacc aagttgcttc cgggtcgtac tttgcaaaaa atggaatatc
     2101 cacccatttt gcatttcgcc cttgtagaaa tcgtagcaca tacacttttt caccttttac
     2161 ttcttgaact ccaagtactt ggattttgcc gggctcacag ctcatgcttg gacctcttac
     2221 cgttctacaa agcccgctta cctgtctgta tgcctttcgg aatatattcc agcatttctc
     2281 caaaggaagc tcaaagtatc gctttgaacc cgtatcgcgt gctacaaaca tataataggg
     2341 aatacatccc agatctacct gctttctcca catttcagcc catatttcgg gtttatcgtt
     2401 gatgttcttt agcaaggggg attgtgtgcg gatttgtgct cctgtagctc gaatttgttt
     2461 gattgccttc ttaaccgcgt cggttttcag ttccgccgga tgattaaaat gtgcctgaac
     2521 agccaaattt ttacctgctt tgattatttg ttcaaacaat ttgaataatt ctttgctgtc
     2581 cttatctttt gtgaagcgat aaggccaata actcagtgct ttggttccaa ttcgaatact
     2641 atgtatattt ctaaattccg gctgcaaaag aacatcgata taggatgcta gaactcgcgt
     2701 gctcatggtc attggatcgc cgcctgtaaa cagaacgtct gtcactttat tattccgaag
     2761 caggtattga gcaaggaggt ttgtctcttt catagcaaat ttcaagttag acattccgct
     2821 gaattgtggc caacgaaagc aaaatgaaca gtaagcatgg caagtttgcc cctgactggg
     2881 gaaaaacaag accgtctcgg aatatttatg ctgaacgcct tttaatttga tgttgtttat
     2941 ttccggtaca ttttgttcct gtccggcggg gtttggattc aattccatgc ggatttcccg
     3001 caccttttta tcgagtgtcg cctgatccgc accttggtcc agtagtttct tcaccgcatt
     3061 ataatgcctt ctttcaagca tctccttcct gggaaaagtg agcgtaaata tcgggtcagt
     3121 atctatttgt gtccaatcga taagctcatc tataacataa ttgtttgtct tgaagggaag
     3181 tacacgtcct acaacttcga ttgcttcgat catttcatcc gataagtttg taatttgcgg
     3241 aatgctttta taattgcgta aggtgtaggt tttgagttga tgtgccatat ctattatgtt
     3301 tttaaacgtt gtaaaacaat acaattgtcc tgtcaggaca aatacaaatg tagcaagttg
     3361 acttttagcg acttacaata aaatagagaa gaagtatgga agcctatttg cctaacaaac
     3421 cgctccgaag ttaaaaaaac aggagaaaac gcaaaagctt cttagcgttt tttacgaatt
     3481 tataaaatat tgtaaaacgt gactacgatc atggccattt tgacggcggt actatgggaa
     3541 tcggcaaata agccaatcgg gattttaaga
//
LOCUS       DAOSTD010000126         3563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_23586_length_3563_cov_11.000855, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000126 DAOSTD010000000
VERSION     DAOSTD010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23586_length_3563_cov_11.000855"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..2558)
                     /locus_tag="PLI37_07255"
     CDS             complement(45..2558)
                     /locus_tag="PLI37_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOQ58842.1"
                     /translation="MKIAKRRNRGLTSYAMGRSLLIIPVLLILFLSGSCSKNFDHYYQ
                     RPSWLEKNALEVLEKKGNFNHYLKLVDRTLYSKVLRGSGHYTFFAPNDQAFEVWLQEN
                     GYDKVEDIPLSLATDIVAYGLIYNAYEAANLGNTWLSSAEWELGSSFRKQTPSYKTIF
                     REEVDGQKRWTLHSTGSFNESWHNYRYLPIFTNNYFTANGLSAADYNEFYPQTNWNPA
                     NGNVLGAEIITPDIYVENGVVHEINKVIVPPANLDELISIRGKDPSNTAQGWALFKEL
                     LYHKLPDSSYQFILYSDDVDAQYYYNKMYPDSAVGSVHLKTYNSEYIGLPLNLEGYHG
                     PTEGTTSENGAYTLFVPDAAAIKSYLSNTLLKYTDDFNKLPSTVLSTFIASHICDGLV
                     WPSNFKLAKNLVGLEGEFINGRGVLGSDFAGSGITSAQFASNGMVYTINKVVKSGFFE
                     SVYGRLLLDPAYSFMNQVISANYNTTVYQYLLQSPYSGYLNENYTLLLPANDLLENDG
                     FTFDPSNSTFSNASASSVGGVDVVARINRLAASGVFMRTKDYPLDNFNTAPAALGDSY
                     GGYGFAVNYFGDMVRYKNNYLQAVGNIADNDSVHLQKDTVNYANGQFYQLDKLINYSP
                     RNTMGGSSEGWNQGSLVSFVQDYLDKSDKTLFKTYWDTCLVEIEGSINTSAFYTVLIP
                     TNQRMQEAIDAGYLPTIEEVNANLNGELIKAVAFINAHLIIGEVYPDDGLDRIFPGNY
                     ASYKTTTSHKITEAKLDLVLAKSYVNISKGADRNQLTFKAADIEAGSNVLVYGRGSTQ
                     GTQQVIRGLNNSNVMGPRAVLHSLDGFVCFEINQTLLSN"
     gene            2952..3434
                     /locus_tag="PLI37_07260"
     CDS             2952..3434
                     /locus_tag="PLI37_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58843.1"
                     /translation="MKTKNILILFLLMLHIGLVSARSLVIELKDGTKMYYLVSLNENP
                     CLVFDQGSMSIQSDRFTISDVNKFYISETDDPNQLNNPRQEETFRSTGLAAESLHIII
                     DNLNSDQLPKIGLFSIDGKSLECSYRFKGKELILETSGLARGIYLLKIDNKSLKFQKK
                     "
BASE COUNT          992 a          819 c          772 g          980 t
ORIGIN      
        1 ttattcagta tatttttcat ccttgccttg ttgatggttt gcacttaatt ggataaaagg
       61 gtttggttga tctcgaaaca gacaaaaccg tccaagctgt gcaagacagc tctgggtccc
      121 ataacattac tgttgtttaa tccgcggatc acctgctgtg ttccctgtgt ggaaccgcgt
      181 ccgtacacca acacattgct gcccgcttcg atatcggccg ccttgaatgt caattggttc
      241 cgatcggccc ctttgctgat gttcacgtaa gatttggcca acaccaaatc gagtttggcc
      301 tcagttatct tgtgcgaagt cgtcgttttg taagaggcgt aatttccggg gaaaatcctg
      361 tccagaccat cgtcgggata aacctccccg ataatcaggt gagcgttgat aaaagcaacg
      421 gcttttatca actctccatt gagattggca ttcacttcct ctatagtcgg caggtaaccg
      481 gcgtcgatgg cttcctgcat gcgctggttt gtgggaatca gcacagtata aaaggcactg
      541 gtatttatag aaccttcaat ttctaccaga caagtgtccc agtaggtctt aaacagggtt
      601 ttgtcgctct tgtccaggta atcttgaaca aaactgacta aactgccctg gttccagcct
      661 tcggacgatc cgcccatggt gttcctgggg ctgtaattga tcaatttgtc caactgataa
      721 aactgaccgt tggcgtaatt gaccgtgtct ttttgcaaat gcaccgaatc gttatcggca
      781 atattgccta cagcctgcag gtagttgttt ttgtagcgaa ccatatcgcc gaaataattc
      841 accgcaaaac cgtaacctcc gtaactgtcg ccgagagcgg cgggcgccgt attgaaatta
      901 tccagagggt aatccttggt tctcataaag actccgctgg cggccagccg gttgattctt
      961 gccaccacat ccactccccc caccgaagag gcgcttgcat tggagaaagt agagttggaa
     1021 ggatcaaaag taaaaccgtc gttttcgagt aaatcgttgg ccggcagcaa taaagtgtag
     1081 ttttcgttca gatagcccga ataaggcgat tgcagcaaat actgataaac ggtagtattg
     1141 taattggccg atatcacctg attcataaaa gaataggcag gatcaagcaa caacctgccg
     1201 tagaccgact caaaaaagcc ggactttacc accttgttga tagtgtagac cataccgttg
     1261 cttgcgaatt gagccgaagt aataccggag cccgcaaaat cactgcccag aacgcctcga
     1321 ccgttgataa attccccctc gagaccgacc aggtttttgg ccaatttaaa attggacggc
     1381 caaaccagtc cgtcgcaaat atgcgaagca ataaaagtgc tcagcaccgt cgagggcaat
     1441 ttgttgaaat cgtcggtgta tttgagcagg gtgttgctca aataactttt aatggcagca
     1501 gcgtcgggta cgaacaaagt ataagcgccg ttttcggaag tcgtgccttc ggtgggaccg
     1561 tgataacctt ccaaattcag cggcaaaccg atgtattcgg aattgtatgt tttgagatga
     1621 accgaaccga cagccgaatc gggatacatc ttattgtaat aatactgcgc atccacatcg
     1681 tcgctgtaca aaataaactg gtaagaactg tcgggcagtt tatgatacaa aagttcctta
     1741 aacaaagccc aaccctgggc agtgttggaa gggtctttgc cccggatgga tataagctcg
     1801 tccagattgg cagggggcac tatcaccttg ttgatttcgt gaaccacccc gttttctaca
     1861 tagatatccg gagtgataat ttctgctccc agcacattac cattggccgg attccaattc
     1921 gtctgaggat aaaattcatt gtaatcggca gcactcaggc cgttggcggt aaaataattg
     1981 ttggtaaaaa tgggcaggta acggtaatta tgccaacttt cgttgaatga gcctgtcgaa
     2041 tgcagagtcc agcgcttctg tccgtccact tcttctctga aaatagtctt gtacgaaggg
     2101 gtctgtttgc ggaacgaact acccaactcc cattcagccg aactcaacca ggtattgccc
     2161 agattggccg cctcgtaggc gttgtaaatc aaaccgtaag caactatgtc tgttgccagg
     2221 ctcaggggaa tgtcttccac cttatcgtag ccgttttcct gcagccagac ctcaaaagcc
     2281 tgatcgttgg gggcaaaaaa agtataatgc cccgaacctc gcagtacttt agaatacaag
     2341 gttctgtcga ccagtttaag gtagtggttg aaatttcctt ttttctccaa cacttcgaga
     2401 gcgttttttt cgagccacga aggacgttgg taataatggt caaaattctt gctgcaagat
     2461 cccgaaagaa acaaaatcaa aagaacgggt attattaaaa ggctccgtcc cattgcgtag
     2521 cttgtaaggc cccggttgcg gcgttttgcg attttcatgt tatttcaggt taatacaaaa
     2581 ttgtaatttt ctgtttgtca agccatctct atgggcttat gcttgtctgt ggatacgttt
     2641 taatttcaag ttttaaattc aggttttaaa taagcataaa tgtaagcata aatgatacaa
     2701 taatatgcaa gaatgagcgt attttggcat aattctactt ttattcacga caagaaatac
     2761 ctacatttgt cttgtttcct ttagttaact gagtgtgagt atttacattt atgacaaatg
     2821 tatgattttt tggaatggtc tgttgtttga ataatgatat ttctattcac aaaactgacc
     2881 taatatttgt taatcaaacc aatgcccaac gataaaaacc cgaaatacat aactatttaa
     2941 atacccttcc gatgaaaacc aaaaacattt tgattctttt tcttttgatg ctccatatcg
     3001 gtttggtttc ggccagaagc ctggtcattg aactcaaaga cggcacaaaa atgtattacc
     3061 ttgtctcact caatgaaaat ccttgcctgg tattcgacca aggcagtatg agcatacaat
     3121 ccgaccggtt caccatctcc gatgtaaata agttttatat cagcgagacc gacgatccca
     3181 atcaactgaa caacccccgg caggaagaga ccttcagaag cacaggcctt gcggccgaat
     3241 cgctccacat tatcatagac aacctgaatt ccgaccaact acccaagatc ggcttgttca
     3301 gcatagacgg aaaatcgctc gaatgttctt accgcttcaa gggcaaggaa ctgatattgg
     3361 aaacctccgg tttggcccga ggcatttatc ttttaaaaat cgacaataag agcctcaaat
     3421 ttcagaaaaa atgaaaaaaa atcttctatt gatactcctg ctgggcttcg gcctgcaaag
     3481 cccggcccaa tcggaactct atgtggtaaa acaagaccgc agctacgaaa gcatcgactt
     3541 gaacaacatc gacagtatcg tgt
//
LOCUS       DAOSTD010000127         3468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_24405_length_3468_cov_10.384706, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000127 DAOSTD010000000
VERSION     DAOSTD010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24405_length_3468_cov_10.384706"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PLI37_07265"
     CDS             <1..585
                     /locus_tag="PLI37_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58844.1"
                     /translation="VGNPNISLILGPGPRLLAAGDTLQADRKAALLDILEEELVPGMK
                     DLIQQTGQVIAQLEAQLNEGGDLNKSLASVRKATEKLASASTSLDLLLTNQIPSAIGN
                     IDSLSNNFNALGKELRTLDFAGISHRVDSSLLILQDLSLRLTRPDNSLGLLLNDTELY
                     FNLNKTASSANELLLDLRERPKRYVHFSLFGSKK"
     gene            993..2396
                     /gene="dnaA"
                     /locus_tag="PLI37_07270"
     CDS             993..2396
                     /gene="dnaA"
                     /locus_tag="PLI37_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOQ58845.1"
                     /translation="MDFILNHEELWSKCLDIVKDIVHESNFSTWLLPIVPLRYENEDL
                     LLEVPSHFFFEHIESHFSDVIGKSLQRITGKPTRLLYKIKLSDNYIDITPDQRPNKEK
                     AVQNLPANTKTSLVSPFEQLLPNRLDPQLNPGFHFGNFIAGNSNKLSRTAGVSIAESP
                     GKTIFNPLFVYGKSGVGKTHLLHAIGNHALYKNPDLRVLYVSSHLFQVQYSQAYLNNS
                     INDFIHFYQSIDLLLIDDIHELSGKQGTQNVFFHIFNHLHQNSKQLVLTCDRAPKDIQ
                     GVEDRLLTRFKWGLTAEITQPDYQLRLDILKSKIQRDGLVFPEEVIEYIAHHAKDNIR
                     DLEGIVVSLMAHSIIIGKNVDLELCKQVVDNSIQVKERPISVELIENEVCKYYNLEPQ
                     LIQTRSKKQEVAHARQIAMYLSKKYTDSSYARIGELLGKRNHATVIHACKTVNNWIET
                     DKGLRSDIQKIEKILLR"
     gene            complement(2455..3192)
                     /locus_tag="PLI37_07275"
     CDS             complement(2455..3192)
                     /locus_tag="PLI37_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOQ58846.1"
                     /translation="MDSMKKRATVRAYSQQDISPELLDELLEIACRSSNTGNMQAYSI
                     VVSRDQERKKALAACHFNQPQVVDAPVVLTFCADFNRFSKWCAQRNALAGFDNIQALT
                     YASIDAVIAAQTFCVAAEEKGLGICYLGTTTYLAQEIIELLELPPLVFPVTTVSVGYP
                     LESPKQCERLPLEAVVHSEAYRDYTPADIDRLHLAKEQLPENRHFVEINHKENLAQVF
                     AEIRYTKKDNEHFSDTLLRAVKKQGFC"
BASE COUNT          990 a          761 c          762 g          955 t
ORIGIN      
        1 gtgggcaacc ccaacatcag cctgattctg ggtccgggtc ctcggttgct ggccgcgggt
       61 gacaccttgc aggccgatcg taaagccgcc ttgctcgata tactggagga agagctcgtg
      121 ccgggcatga aggatttgat tcaacaaacc ggccaggtga ttgcccaact cgaagctcaa
      181 ctgaacgaag gaggcgacct gaacaaaagc ctggcctcgg tacgcaaagc gaccgaaaag
      241 ctggcctctg catcgaccag tctcgatttg ctgctgacca accaaatccc ctcggccatc
      301 ggaaacatcg attccctgag caataatttc aatgccctgg gcaaggaact cagaaccctt
      361 gatttcgccg gcatcagcca ccgtgttgat agcagcttac tgatactaca agacctgagc
      421 ctgcgcctga ccaggccgga caacagcctg ggtttattgt tgaacgatac tgaattgtat
      481 ttcaatctga acaaaactgc ctcgagcgcc aacgaactcc tgctcgattt aagagagcga
      541 cctaaacgtt acgttcattt ttcgctcttt ggttccaaaa aataacagta ggcggaaaaa
      601 atcaggtaga gagaaaaaat aaagtcggaa gattaataac aatgcccgag aaatttatac
      661 ggaaaattcg ggctgttttt agaattaaat agcaggtttt aaaacttaat gtaaaaataa
      721 tacaagtccg ttattccctt tgccctaaag cgattggagg atttgctgac aatttaacaa
      781 ttcaaactgt caaaattaca aagctctatt tataagattg ttgtgattta attttttaaa
      841 caaattgctg actattagtg ctttatgcct aatcggttaa ggctaaaaca gatacaactt
      901 tgcaaaactt ttgaacaaaa tcttcgtttg ttttattcgt tttttcttca aaaatttgca
      961 gtgcgattca agctaaactc agaggaataa ttatggattt tattttaaac catgaagaat
     1021 tgtggagcaa atgcctcgac attgtcaagg acattgtgca tgaatccaat ttctctacat
     1081 ggttgctccc catcgttcct cttaggtatg aaaacgagga tttgctcctg gaagttcctt
     1141 cgcatttctt ttttgagcac atagaaagtc atttttcgga tgtgatcggt aaaagcctgc
     1201 agcgtattac aggcaagccc acccggctgc tatacaaaat taaattgtcg gacaattaca
     1261 tcgatatcac acccgatcaa cgccccaata aagaaaaagc cgtacagaat ctgccggcca
     1321 acaccaagac ctcgctggta agtcctttcg aacagttgtt acccaacagg ctggatcctc
     1381 agcttaatcc cggttttcat ttcggaaact ttattgccgg caacagcaat aaactttcgc
     1441 gtacggcagg cgtttcgata gccgaaagtc cgggcaaaac catttttaat ccgctgtttg
     1501 tttatggtaa gtccggtgtg ggaaaaaccc atcttttgca tgctataggc aatcacgcac
     1561 tctataaaaa tcccgatctg agggtgcttt atgtttcctc gcatttgttt caggtacaat
     1621 attcacaggc ttatctcaac aacagcatca acgactttat tcatttttac cagagcatcg
     1681 acttgctgtt gatagacgat attcatgagt tgtcgggtaa acagggaacg caaaatgtgt
     1741 ttttccatat tttcaaccac ctgcatcaaa acagcaagca actggtgctg acctgcgaca
     1801 gggcacccaa ggatatacag ggagtggaag atcgcttact gaccaggttt aagtgggggc
     1861 ttacggccga aatcacccag cccgattacc aattgcgatt ggatattctc aaaagtaaaa
     1921 tccaacggga tggtttggta tttcccgaag aagtgataga atatattgcc catcacgcca
     1981 aagacaatat ccgtgacctg gaaggcatcg tagtgtcgtt gatggctcat tccatcatta
     2041 ttggtaaaaa cgtcgatttg gaactctgca aacaggtagt ggacaattcc atccaggtaa
     2101 aagaacgccc catttcggtg gaactcatag aaaatgaggt gtgcaaatac tataatttgg
     2161 aaccccaact gatacaaacc cgctccaaaa agcaggaggt ggctcacgcc cggcagattg
     2221 ccatgtatct gtccaaaaag tacaccgaca gctcctacgc ccgaataggc gagctgctgg
     2281 gcaagcgcaa tcacgctacc gtgatccatg cctgcaaaac agtaaacaac tggatagaga
     2341 ccgataaagg tttacgcagc gatattcaaa aaatcgaaaa gattttgtta cggtaaatat
     2401 ccgagagttt cccggtctaa agcaaacttc aggaattaat cttaaagcag ggcttcaaca
     2461 gaaaccctgc ttttttactg cccggagcag tgtatcggag aaatgttcgt tgtctttttt
     2521 cgtataacgg atttcggcaa atacctgagc caggttttct ttatgattga tttcaacaaa
     2581 atgccggttt tcgggcaatt gttctttggc caggtgcaga cggtcgatgt cggcgggcgt
     2641 ataatcgcgg taggcttcgg aatggacgac ggcttccagc ggaagacgct cgcattgttt
     2701 gggagactcg agaggatagc ccacactgac ggtggtcacc ggaaaaacca agggcggcaa
     2761 ttcgaggagc tcgattattt cttgggccag gtaagtggtt gtacccagat aacaaatgcc
     2821 aaggcctttt tcctcggcgg caacgcaaaa agtctgggct gcaatgacgg cgtcgataga
     2881 ggcgtaggtc agggcctgaa tattgtcaaa tccagccagg gcattgcgct gggcacacca
     2941 tttgctgaaa cggttgaaat cggcgcaaaa agtcaaaacc accggtgcat cgaccacctg
     3001 gggctgattg aaatgacagg ctgcgagggc ttttttgcgt tcctgatcgc ggcttaccac
     3061 gatgctgtag gcttgcatgt tgccggtatt ggaagaacgg caggcaattt cgagtaactc
     3121 atcgagcagc tccgggctaa tatcttgttg ggaataggca cgtacagtgg ctcttttttt
     3181 cattgaatcc atagaattta atttaggctg tgattttttt ctttgcaaag atagggtttt
     3241 aaaaaaatct tcccaattat tttttttatc aacttggtta atactatttt tgttgaaaat
     3301 tttcattttt ggtcattctc tcatttattt tactttttta ttattcacat tatctgacac
     3361 ttacaagttt ttgatttgtc atttgtaaga attaataata tgttcatgtt gaattttttt
     3421 ttcacttgat tatttgagct aatattgcaa actcaataga acaaaccc
//
LOCUS       DAOSTD010000128         3464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_24444_length_3464_cov_10.721326, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000128 DAOSTD010000000
VERSION     DAOSTD010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24444_length_3464_cov_10.721326"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PLI37_07280"
     CDS             complement(<1..688)
                     /locus_tag="PLI37_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOQ58847.1"
                     /translation="MIVTVKELDRAVVRFSGDSGDGMQLTGTLFSTLSAIFGNEISTF
                     PDYPAEIRAPQGTLSGVSGFQVHIGNKVYTPGDKADVLVAMNPAALKVNVKNIKKDAV
                     ILVDADAFDESGLKKALYKTEDPIEELKLGAAQLVAVPISTLVKESLADTGMGNKEIL
                     RSKNMFALGLICWLFNKPIEEAMRLLHRKFGKKPGVLQANIKVLTDGFNYGHNIHASV
                     STYNIRPTDMP"
     gene            761..1702
                     /gene="nadA"
                     /locus_tag="PLI37_07285"
     CDS             761..1702
                     /gene="nadA"
                     /locus_tag="PLI37_07285"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOQ58848.1"
                     /translation="MDREVLKSEIKRLRSERNAVIMAHYYQIREIQEIADFVGDSLDL
                     AQQAAKTDADVIVLCGVHFMAETAKIISPNKKVLIPDAGAGCSLAESCPAGEFEAFIK
                     AHPDHTVISYVNTSAAVKALSDLVVTSSNALQIINSLPQDEKIIFGPDRNLGNYIKSL
                     SGRDMLIWDGACHVHEQFSLQKILELKKQHPQAEIISHPECKQDLLKVSDFIGSTSAQ
                     VAYVQKAEAGEFIVATECGVLFEMSRKCPGKLFIPAPPDDDSKACNECTFMRMHNLEK
                     LYRCLRDEQPEVLVEEQLRRQALRSIQRMLNISANLK"
     gene            1711..2307
                     /locus_tag="PLI37_07290"
     CDS             1711..2307
                     /locus_tag="PLI37_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOQ58849.1"
                     /translation="MLKVVFASHNKHKKQEVADILGNSVELIDLEDLNCSEDIPETQD
                     SLQGNALQKARFVHEKYGLDCFADDTGLEVEALNGAPGVHSARYGGPACNFEDNMDKL
                     LLELQGKENRRARFRTVVALLLDGREYFFEGRVEGEILRQKAGNKGFGYDPLFRPEGY
                     ELSFAQLEPEQKNRISHRAEAVQKMARFLETYKRNLQK"
     gene            2366..>3464
                     /locus_tag="PLI37_07295"
     CDS             2366..>3464
                     /locus_tag="PLI37_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58850.1"
                     /translation="MKKRLCLLLLSGLAGVLCFKLAAQPPVGEWRSHFSYRNATQVAA
                     TSDKVYVVANKHLFSYSPRDRFIEEYSVLTGLSGGQVQFIAQNDVSDCLLVVYTDGNM
                     DILDDERVINLPDYKEKSLPADKSIYRLRMDGRYAYLCTGVGLLIIDVPKQEILETYN
                     LRSATVNYTAVFDFARIGDDYYMLTERGLYRGNAGDNLSDSKNWTAENFIPGKTPAQL
                     VSRKDSLYALVAGEGIYRYDGGWKAFYLSAGVLEISAQGPYLLALTAGLPLVFAADNS
                     QLTLHDSDVSGEYSLYEYNAQMQAGLKQMYGIALDEKLNRIYTTAGSDGLLEWIYYAQ
                     AEAYMVSNSKILPNGPGVDTAWDLYFRNNDLY"
BASE COUNT          931 a          752 c          885 g          896 t
ORIGIN      
        1 tgggcatgtc ggtgggacga atattgtagg tcgacacgga tgcatgaatg ttgtgcccgt
       61 agttgaatcc gtcggtgagc actttgatgt tggcttgcaa aacacccggt tttttgccga
      121 attttcgatg gagcaaacgc atggcttctt cgatgggttt attgaagagc cagcaaataa
      181 gccccagggc aaacatgttt ttactgcgta aaatttcttt attacccatg ccggtgtcgg
      241 ccagactttc tttgaccaga gttgaaatgg ggacagccac aagttgcgct gctcccaatt
      301 tgagctcttc tatagggtct tccgttttat aaagggcttt ttttaggccg ctttcgtcga
      361 aagcatcggc atcaaccaag ataacagcgt cttttttgat gttttttacg tttactttga
      421 gagctgccgg attcatggca accagtacat cggctttgtc gccgggagta tacactttgt
      481 ttccgatgtg tacctggaaa cccgacaccc cgctcaaagt gccttgggga gcccgtattt
      541 cggccggata atcgggaaaa gtggaaattt cgtttccaaa aatagcagac agggtagaga
      601 acagggttcc ggtaagttgc atgccgtctc ctgagtcgcc cgagaagcgt acaacggccc
      661 tgtctaattc tttaaccgtt acaatcataa ctaatactga aaaaaacgca gcaaaggtac
      721 ggtttaattt tatatttttg cggctgtaaa aaggctgatt atggatagag aagtgttaaa
      781 atcagaaata aagcggctta gatctgaaag gaatgcggta atcatggcgc attactatca
      841 gataagagag attcaggaaa tagcagattt tgtgggagac agcttggatc tggcacagca
      901 ggctgccaaa acagacgccg atgtcattgt gctttgcggg gtacatttta tggctgagac
      961 agctaaaata atttcaccaa acaaaaaagt cctgattccc gatgccggag cgggttgttc
     1021 tctggccgag agttgtccgg ccggcgaatt tgaagctttt atcaaggctc atcccgatca
     1081 tacggttatc agctatgtca atacttcggc ggctgtaaaa gctctgagcg acctggtggt
     1141 aacttcgagt aatgccctgc aaatcataaa tagcctgcca caagacgaaa agattatttt
     1201 tggacccgac cgtaacctgg ggaattatat taaaagtttg agcggacgcg atatgcttat
     1261 ctgggacgga gcctgtcatg tacatgaaca attttcactg caaaaaattc tggaactcaa
     1321 aaagcaacat cctcaggctg aaattatttc acaccccgaa tgcaaacaag acttgttgaa
     1381 ggtctccgat tttatcggtt ctacctcagc ccaagtagcc tatgtacaaa aagccgaagc
     1441 aggggaattt attgtagcca ccgagtgtgg tgtgttgttt gaaatgtcgc ggaaatgtcc
     1501 cgggaagtta ttcataccgg ctcctcctga cgatgactcc aaagcctgta acgaatgtac
     1561 ctttatgcga atgcataacc tggaaaagct ttatcgctgc ctccgggatg aacaaccgga
     1621 ggttttggtg gaagagcaat tgcgccggca agccctcagg tctatccaac gaatgctcaa
     1681 catttcggca aacctcaaat aaattttcgt atgttgaaag ttgtatttgc cagccataat
     1741 aagcacaaga aacaggaagt ggccgatatt ctggggaatt ccgtcgagtt gatcgatctg
     1801 gaggatttga attgcagcga agatattccc gaaactcagg atagtctgca gggcaacgct
     1861 ttgcaaaagg ctcgttttgt acacgaaaaa tacggtctgg attgctttgc cgacgatacc
     1921 ggactcgaag tggaagccct gaacggagca ccgggagtcc attcggcccg ttatggcggc
     1981 cccgcctgca attttgaaga taatatggat aagttgctct tggaattgca gggcaaagaa
     2041 aaccgcagag cacgtttcag aactgtggtg gccttgttgc tcgatggccg ggaatatttt
     2101 ttcgaaggcc gggtcgaagg cgaaattttg aggcaaaagg cgggtaacaa aggttttggt
     2161 tatgatcctt tgtttcgccc cgagggttat gaattgagtt ttgcccaact ggagccggaa
     2221 caaaaaaaca ggatcagcca tagggcagag gctgtgcaaa aaatggcccg ttttttggaa
     2281 acctataaaa gaaacctaca aaaataaaca agataaaccc cggttctgta caatattcga
     2341 cccttgtttg cggttattgc ttgtgatgaa aaagagactc tgtttattgc tgttgtccgg
     2401 acttgccggt gtactgtgct ttaaacttgc agcccagccg cccgttggag aatggcgcag
     2461 ccatttttcg taccggaatg ccactcaggt ggccgctacc tccgacaaag tatacgtggt
     2521 ggccaacaaa catttgtttt cgtattcgcc ccgggatcgt tttatcgaag aatattccgt
     2581 tttgaccgga cttagcggag gccaggtgca gtttatagcc caaaacgatg ttagtgattg
     2641 tttgctggtg gtatacaccg acggcaacat ggatatattg gacgacgaaa gggtgatcaa
     2701 tctgccggat tacaaggaaa aaagcctgcc cgccgacaaa agcatctacc ggcttcgcat
     2761 ggacggcagg tatgcctatc tgtgtaccgg agtcggcttg ttgataattg atgtacctaa
     2821 gcaggaaatt ctggaaacgt acaacctgcg aagtgctacg gtaaattata cggcggtgtt
     2881 cgactttgcg cgtatcggcg acgattatta tatgctcacc gaacgaggcc tttaccgggg
     2941 aaatgccggc gacaacctgt cggacagcaa aaactggaca gcagagaatt ttataccggg
     3001 taaaaccccc gcacaactcg tttcacgcaa agattccctt tatgcattgg tggccggaga
     3061 gggcatttat cgctacgatg gaggctggaa agcattttat ttaagtgccg gtgtgctgga
     3121 aatatcggca cagggcccat acttgctggc tctgaccgct gggctgcctt tggtttttgc
     3181 agcggacaac agtcagttaa ctctccacga cagcgatgtg tcgggagaat attctttgta
     3241 tgaatacaat gcacagatgc aagccggact aaagcaaatg tatggcattg ccctggatga
     3301 aaagctgaac cgaatttata ccacggccgg cagtgacggc cttctcgaat ggatttatta
     3361 cgcccaggcg gaggcctata tggtgagtaa cagtaaaatc ctgcctaatg gcccgggtgt
     3421 ggacacagcc tgggatttgt atttccgcaa caacgatttg tatg
//
LOCUS       DAOSTD010000129         3428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_24783_length_3428_cov_11.200415, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000129 DAOSTD010000000
VERSION     DAOSTD010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24783_length_3428_cov_11.200415"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="PLI37_07300"
     CDS             complement(<1..1066)
                     /locus_tag="PLI37_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58851.1"
                     /translation="MKKRLCLLLMSGLAGVLCFKVAAQPPVGEWRSHFSYRNATQVAV
                     TSDKVYVVANKHLFSYSPRDRFIEEYSVLNGLSGGQVQFIAQNEVNDCLLVVYTDGNM
                     DILDGERVINLPDYKEKSLPADKSIYRLRMDGRYAYLCTGVGLLIIDVQKQEILETYN
                     LRNATVNYTAVLDFARIGNDYYMLTERGLYRGNAGDNLSDSKNWTAENFIPGKTPAQL
                     VSRKDSLYALVAGEGIYRYDGGWKAFYLSAGVLEISTQGPYLLALTSGLPLVFAADNI
                     LLTLHDSDVSGEYSLYEYDAEMQAGLKRMYGVALDEKLSRIYTTAGSDGLLEWIYHAQ
                     AEAYMVSNSKILPNGPGVDTA"
     gene            complement(1131..1718)
                     /locus_tag="PLI37_07305"
     CDS             complement(1131..1718)
                     /locus_tag="PLI37_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOQ58852.1"
                     /translation="MLKIVFASHNKHKKQEVADILGNSVELIDLEDLNCSEDIPETQD
                     SLQGNALQKARFVHEKYGLDCFADDTGLEVEALNGAPGVHSARYGGPACNFEDNMDKL
                     LLELQGKENRRARFRTVVALLLDGREYFFEGRVEGEILRQKAGSKGFGYDPLFRPEGY
                     ELSFAQLEPEQKNRISHRAEAVQKMARFLGTYKKL"
     gene            complement(1727..2668)
                     /gene="nadA"
                     /locus_tag="PLI37_07310"
     CDS             complement(1727..2668)
                     /gene="nadA"
                     /locus_tag="PLI37_07310"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOQ58853.1"
                     /translation="MDREVLKSEIKRLRSERNAVIMAHYYQLEEIQEIADFVGDSLEL
                     AQQAAKTDADIIVLCGVHFMAETAKIISPGKKVLIPDAGAGCSLAESCPADEFEAFVK
                     AHPDHTVISYVNTSAAVKALSDLVVTSSNALQIINSLPQDEKIIFGPDRNLGNYIKSL
                     SGRDMLIWDGACHVHEQFSLQKTLELKKQHPQAEIIAHPECKQDLLKVSDFIGSTSAQ
                     VAYVQKAEAREFIVATECGVLFEMSRKCPGKLFIPAPPDDDSKVCNECVFMRMHNLEK
                     LYRCLRDEQPEVLVEEQLRRQALRSIQRMLDISASLK"
     gene            2741..>3428
                     /locus_tag="PLI37_07315"
     CDS             2741..>3428
                     /locus_tag="PLI37_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOQ58854.1"
                     /translation="MIVTVKELDRAVVRFSGDSGDGMQLTGTLFSTLSAIFGNEISTF
                     PDYPAEIRAPQGTLSGVSGFQVHIGNKVYTPGDKADVLVAMNPAALKVNVKNVKKDAV
                     ILVDADAFDENGLKKALYKTEDPIKELNLGSAQLVAVPISTLVKESLADSGMGNKEIL
                     RSKNMFALGLICWLFNKPIEEAANLLQRKFGKKPGVLQANIKALTDGFNYGHNIHASV
                     STYNIRPTDMP"
BASE COUNT          864 a          916 c          768 g          880 t
ORIGIN      
        1 aggctgtgtc cacacccggg ccgttaggca ggattttact gttactcacc atataagcct
       61 ccgcctgggc gtgataaatc cattcgagaa ggccgtcact gccggccgtg gtataaatcc
      121 ggctgagctt ctcatccagg gcaacgccat acattcgctt cagtccggcc tgcatctctg
      181 catcgtattc atacaaggaa tattcacccg acacatcgct gtcgtggaga gtcaacagaa
      241 tgttgtctgc tgcaaagacc aaaggcagcc ccgacgtcag ggccaacaga tatgggcctt
      301 gtgtggatat ttccagcaca ccggcactta aataaaaagc tttccagcct ccgtcgtaac
      361 gataaatgcc ctctccggcc accaatgcat aaagggaatc tttgcgcgaa acgagttgcg
      421 cgggggtttt acccggtata aaattctctg ctgtccagtt tttgctgtcc gacaggttgt
      481 cgccggcatt tccccggtaa aggcctcgtt cggtgagcat ataataatcg ttgccgatac
      541 gcgcaaaatc gagcaccgcc gtataattca ccgtagcgtt gcgcaggttg tacgtttcca
      601 ggatttcctg cttttgtaca tcaattatca gcaagccgac tccggtacac aagtaagcat
      661 acctgccgtc catgcgaagc cggtagatgc ttttgtcggc aggtaggctt ttttctttgt
      721 aatccggcag attaattacc ctttcgccgt ccaaaatatc catgttgccg tcggtatata
      781 ccaccagcaa acaatcgttg acttcgtttt gggctataaa ctgcacttgg cctccactga
      841 gtccgttcaa aacggaatat tcttcgatga aacgatcccg gggcgaatac gaaaacaaat
      901 gcttgttggc caccacgtat actttgtcgg aggtaacggc tacctgggtg gcattccggt
      961 acgaaaaatg actgcgccat tccccaacgg gcggctgagc tgcaactttg aagcacagca
     1021 caccggcaag tccggacatg agcaataaac agagtctctt tttcatcgca agcaataacc
     1081 gcaaacaagg gccgaatatt gtacaggcca ggggtttatc tcatttattt ttacagtttt
     1141 ttgtaggttc ccaaaaaacg ggccattttt tgcacagcct ctgccctgtg gctgatcctg
     1201 tttttttgtt ccggctccag ttgggcaaaa ctcaattcgt aaccctcggg gcgaaacaaa
     1261 ggatcgtaac cgaaaccttt gctgcccgct ttttgcctca aaatttcccc ttcgacccgg
     1321 ccttcgaaga aatattcccg gccatcgagc aacaaggcca ccactgttct gaaacgtgct
     1381 ctgcggtttt ctttgccctg caattccaag agcaacttat ccatattatc ttcaaaattg
     1441 caggcggggc cgccataacg ggccgaatgg actcccggtg ctccgttcag ggcttccact
     1501 tcgagtccgg tatcgtcggc aaagcaatcc agaccgtatt tttcgtgtac aaaacgagcc
     1561 ttttgcaaag cgttgccctg cagactatcc tgagtttcgg gaatatcttc gctgcaattc
     1621 aaatcctcca gatcgatcaa ctcgacggaa ttccccagaa tatcggccac ttcctgtttc
     1681 ttgtgcttgt tatggctggc aaatacaatt ttcaacatac ggaaatttat ttgaggcttg
     1741 ccgaaatatc gagcattcgt tggatagacc tgagggcctg ccggcgcaat tgctcttcca
     1801 ccaaaacctc cggttgttcg tcccggaggc atcgataaag cttttccagg ttatgcatcc
     1861 gcataaagac acattcgttg cagactttgg agtcatcgtc gggaggagcc ggtatgaata
     1921 atttcccggg acatttccgc gacatctcaa acaacacacc acactcggtg gctacgataa
     1981 attcccgggc ttcggctttt tgaacatagg ctacctgagc cgaggtagaa ccgataaaat
     2041 cggagacctt caacaagtct tgtttgcatt cggggtgtgc aataatttca gcctgaggat
     2101 gttgcttttt gagttccagt gttttttgca gtgaaaattg ttcgtgtaca tgacaggctc
     2161 cgtcccagat aagcatatcg cgtccgctca aacttttgat gtaattcccc aggttacggt
     2221 cgggaccaaa aataattttt tcgtcttggg gcaggctgtt tatgatttgc agggcattgc
     2281 tcgaagttac cacaaggtcg ctcaaagcct taacagccgc cgaagtattg acataactga
     2341 tcaccgtatg atcgggatga gccttgacaa aagcttcaaa ttcgtcggcc ggacaactct
     2401 cggccaggga acaacccgct ccggcatcgg gaatcaggac cttctttccg ggcgaaatga
     2461 ttttggctgt ctcagccata aaatgcaccc cgcaaagcac aatgatgtcg gcatccgttt
     2521 tggcagcctg ctgtgccaat tccaggctgt ctcccacaaa atcagctatc tcctgaattt
     2581 cctctaactg atagtaatgc gccatgatca ctgcattcct ttcagaccta agccgcttta
     2641 tttctgattt taacacttct ctatccataa tcagcctttt tacagccgca aaaatataaa
     2701 attaaaccgt acctttgctg cgtttttttc agtattagtt atgattgtaa cggttaaaga
     2761 attagacagg gccgtcgtac gcttctccgg cgattcgggt gacggcatgc aacttaccgg
     2821 aaccctgttc tcaaccctgt ccgccatttt tggaaacgaa atctccactt tccccgatta
     2881 cccggccgaa atacgggctc cccaaggcac tttgagcggt gtgtcgggtt tccaggtaca
     2941 catcgggaac aaagtatata ctcccggcga caaggccgat gtgttggtcg ccatgaatcc
     3001 ggcggccctc aaagtcaacg taaaaaacgt caaaaaagac gctgttattt tggttgatgc
     3061 cgatgctttc gacgaaaacg gcctaaaaaa agccctttat aaaacagaag accctataaa
     3121 agagctcaat ctgggatcag cacaacttgt ggccgtcccc atttcgaccc tggtcaaaga
     3181 aagcctggcc gacagcggca tgggtaacaa agagatttta cgcagcaaaa acatgttcgc
     3241 cctggggctt atctgctggc tcttcaacaa gcccatcgaa gaagccgcga atttgctcca
     3301 gcgaaaattc ggcaaaaaac cgggcgtctt gcaagccaac atcaaagcgc ttaccgacgg
     3361 attcaactac ggacacaaca ttcatgcatc cgtgtcgacc tacaatattc gtcccaccga
     3421 catgccca
//
LOCUS       DAOSTD010000130         3380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_25232_length_3380_cov_17.358797, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000130 DAOSTD010000000
VERSION     DAOSTD010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25232_length_3380_cov_17.358797"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..2642
                     /locus_tag="PLI37_07320"
     CDS             111..2642
                     /locus_tag="PLI37_07320"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOQ58855.1"
                     /translation="MEKKIYTREEAFDASLEYFQGDELAARVWVNKYAIKDSFGNLYE
                     KSPEDMHWRLANEIARIENTYPNPLSAKELFDLFDRFRYIIPQGSPMTGIGNEFQVAS
                     LSNCFVIGIDGEADSYGAVIKIDEEQVQLMKRRGGVGHDLSHIRPKGTPVKNSALTST
                     GIVPFMERYSNSTREVAQDGRRGALMLTVSIKHPDSESFIDAKMTEGKVTGANVSVKL
                     TDEFMRAATENLPFVQQYPVNASDPDITKEINAKALWKKIVHNAWRSAEPGVLFWDTI
                     LNESVPDCYADLGFRTVSTNPCGEIPLCPYDSCRLLAINLYSYVNKPFTTEASFDYEL
                     FKKHVALAQRIMDDIIDLEMEKIDRILEKIDSDPEDPEIKRAERILWEKIKAKTAQGR
                     RTGVGTTAEGDMLAALNLRYGTEEASDFSEKIHRLVAIEAYRSSINLAKERGAFSIYN
                     YEREENNPYIKRLFNEAPELKEDMKAYGRRNIACLTIAPTGTTSLMTQTTSGVEPAFS
                     LIYKRRRKINPNDPGARVDFKDENGDCFEEYIVFHHKFVTWMEAQGYPTAKNYTQEEL
                     DEMIAKSPYYKATSKDVDWMQKVKMQGRIQKWVDHSISVTINLPADVSEELVNQLYIE
                     AWRSGCKGCTVYRDGSRSGVLLDASEKKDKGRLICFPELTPKRPRELIADVLRFQNNK
                     EKWIAFIGMQNGRPYEIFTGIEDDDEGIALPKNVTKGKIIKNFDDHGVKRYDFQFSNR
                     RGYKTTVEGLSDKFNPEYWNYAKLISGVLRYGMPIDQVIKLVGSLELDSKSINTWKNG
                     VERALKNYLPDGSEVQGEKCPKCGNTLVYQEKCAHCVNCDFSRCA"
     gene            complement(2720..3082)
                     /locus_tag="PLI37_07325"
     CDS             complement(2720..3082)
                     /locus_tag="PLI37_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOQ58856.1"
                     /translation="MSRDKTVSNGVIHNAIAPGTQITGSIIAEKDFRIDGNIDGDIVC
                     QGKVVVGRQGYVKGRMACINAEIVGQVDGKIQASEILTLRATAVVQGEIKISTLIVEP
                     NALFNGTCEMFRKDAPAE"
BASE COUNT          999 a          806 c          718 g          857 t
ORIGIN      
        1 tttttttttc acttgattat ttgagctaat attgcaaact caatagaaca aacccaaaac
       61 ttttgcagaa gaatcaaaaa tttcatcaac ttatataacc actttttatc atggaaaaaa
      121 aaatctacac acgggaggag gcttttgatg cctcgcttga gtatttccaa ggcgatgaac
      181 tggctgccag agtctgggta aacaaatacg ccataaaaga ttcctttggc aatttgtacg
      241 aaaaatctcc tgaagatatg cattggcgcc tggccaacga aattgcccgg atcgaaaaca
      301 cctaccccaa tcctctttcg gctaaagaac ttttcgatct cttcgatcgt ttccgttaca
      361 ttattcctca aggcagcccc atgaccggca tcggaaacga gtttcaggta gcctcgctgt
      421 ccaactgttt tgtaattggt atcgacggag aagccgattc atacggagct gtcatcaaaa
      481 ttgacgaaga gcaggttcaa ctgatgaaaa gacgcggcgg agtcggccac gatctttcgc
      541 acattcgccc caaaggcacg ccggtgaaaa attccgcgct gacctccaca ggtatcgtgc
      601 cttttatgga gcgttattcc aattcaaccc gcgaagtagc ccaagacggc agacgtggcg
      661 ccctgatgct gacggtatcc attaaacatc ccgactcaga atccttcatt gacgctaaaa
      721 tgaccgaagg caaagtgacc ggagccaatg tctcggttaa actgacggat gaatttatgc
      781 gagccgccac agaaaacctc ccttttgtgc aacaataccc cgttaacgca tccgacccgg
      841 atattaccaa agaaatcaac gcaaaagctt tgtggaaaaa aattgttcac aacgcctgga
      901 gatcggccga acccggggta ctgttttggg atactatatt gaacgaatcg gtacccgatt
      961 gttatgccga tttgggattc cgtacggttt ccaccaatcc ctgcggtgaa attcctcttt
     1021 gcccctacga cagttgccgc ctgttggcca tcaacctgta ttcctatgta aataaacctt
     1081 ttacaacaga agcaagtttt gattatgagc ttttcaaaaa acacgtggcg ctggctcaac
     1141 gcatcatgga cgatatcatc gatctggaaa tggaaaaaat cgacaggata ctcgaaaaga
     1201 ttgattccga tcccgaagac cccgaaatta aacgcgcaga acgcatactc tgggaaaaga
     1261 tcaaagccaa aacagcccaa ggccgccgaa ccggtgtggg tactactgcc gaaggcgata
     1321 tgctggcggc tctgaatctg cgttacggca ccgaagaggc ctccgatttt tccgaaaaaa
     1381 ttcatcgcct ggtggccatc gaagcttatc gttcttcgat aaaccttgcc aaagaacgcg
     1441 gagctttcag tatttataat tatgaacgtg aagaaaacaa tccctatata aaacgactgt
     1501 tcaacgaagc tcccgaactt aaagaagata tgaaagccta tggcaggcgc aatattgcct
     1561 gtctgaccat tgctcccacc ggcacaacca gcctgatgac tcaaaccact tcgggcgtag
     1621 aaccggcttt cagtctcatc tataaacgcc gccgcaaaat caaccccaac gatcccggag
     1681 cacgggtaga ctttaaagac gaaaacggag attgcttcga agaatacatt gttttccacc
     1741 ataagtttgt cacctggatg gaagctcagg gttaccccac ggccaagaac tatacacagg
     1801 aagaactgga tgaaatgata gccaaatcgc cctattataa agccacttcc aaagatgtcg
     1861 actggatgca gaaagtcaag atgcagggac gtatacaaaa atgggtggac cattccatca
     1921 gtgtgaccat aaacctgccc gccgatgtca gcgaagaatt ggtcaaccaa ctctatatag
     1981 aagcctggcg ctcgggctgt aagggctgca ccgtttaccg cgacggttcc cgttcgggtg
     2041 tactgctcga tgcctcagaa aaaaaggaca aaggaagact gatctgcttc ccggaactta
     2101 cgcctaagcg cccccgggaa ctgattgccg acgtactccg ttttcagaac aacaaagaga
     2161 aatggattgc ctttatcggt atgcaaaacg gacgtcctta cgaaatcttt accggtatcg
     2221 aagacgacga cgaaggcata gctttaccca aaaacgtcac taaaggaaag attatcaaaa
     2281 actttgacga ccatggagtg aaacgctatg actttcagtt tagcaatcgc cggggttaca
     2341 aaaccaccgt tgagggtttg tcggataaat ttaatccgga atactggaac tatgccaagt
     2401 tgatttccgg cgtattacgt tacggcatgc ccatcgatca agtaataaaa ctagtgggca
     2461 gtctggaact cgacagcaaa tctataaata cctggaaaaa cggagtggaa cgtgccctga
     2521 aaaattattt acccgatggt tccgaagtac aaggagaaaa atgtcccaaa tgcgggaata
     2581 ctcttgttta ccaggagaaa tgcgcccatt gcgtcaattg cgatttttcg cgttgcgcat
     2641 agtggtcttt gcaatcggct aataaaaaaa cggctgaaac ttataggttc agccgttttt
     2701 tatttatcgc aacaatactt tattcagcag gagcatcttt tctaaacatt tcgcaggtcc
     2761 cgttgaatag agcattgggc tccactatca gggtggaaat tttgatttcg ccttgaacta
     2821 cggctgtggc tctgagagtc agtatttccg atgcttgtat tttaccatct acctgcccta
     2881 caatctctgc attaatgcat gccatcctgc cctttacata tccttgccgg ccaacaacga
     2941 ctttaccctg acaaacaata tctccgtcaa tgttgccatc tatgcgaaaa tctttttctg
     3001 caatgatact tccggttatt tgagttcccg gggcgatagc gttatgtata actccattgc
     3061 tgacagtctt gtctcttgac ataaaagtta atttaattgt gtgaagccgt tttgattgtg
     3121 tgcaaatata gtattaaata tctaatttgt tcgttgtgct gagtcaaatt ataaaaaatc
     3181 gtacttttgc cgtattaatc aagactcttt caaaacctca ttatggatta cctgctactg
     3241 aacgaaatga aattttttgc ctttcatggc gtaaacgaac aagaacgcat agtgggcaat
     3301 acttatttgg tcagcctcaa gcttggcctg gatatgagca gagcctgcca aagcgaccaa
     3361 cttgaagaca cactcaatta
//
LOCUS       DAOSTD010000131         3367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_25376_length_3367_cov_11.931461, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000131 DAOSTD010000000
VERSION     DAOSTD010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25376_length_3367_cov_11.931461"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1856
                     /locus_tag="PLI37_07330"
     CDS             <1..1856
                     /locus_tag="PLI37_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ58857.1"
                     /translation="DRDKLRLLSEKSYSDDTDLREAEVKSDLEAACITKNRRPSVETS
                     MHNAINYAFVVHLHPTAVNGLMCSRQAENKLRELFGDEHLYIPYTDPGYVLFKKVEGA
                     IAAFRAKRGKEPSVIWLQNHGIFVAADNIEEIRRTYDHILAVLDKEVKTAAHRAAAGS
                     KALQAYLQPEERPVPGRVIEILPALRMLVSETDEQKHIQLKTLKIRNNKLIEYYCEKK
                     EHYIYISRPFTPDGIVYCKSNQLYIDKAKTEDILDDAKKILPFFRQHFGYQPKVIALR
                     GLGIVAVGDNAAQCDTILDVFEDCIKIAAISEAFGGPHPMTQEQIDFIDHWEVENYRR
                     SIAAGTLTGRAQNKTIVVTGAAQGFGRGIADELLKEGANIVVADLNSETGLATVDEFN
                     QKARSNRAVFVQTDVSKVASIEQLVYQSICCFGGIDCFISNAGILRAGGLEEMSPENF
                     DLVTKINYNAYFYCTKVVSRVMKLQTAYGSPDYYADIIQINSKSGLRGSRANFAYAGG
                     KFGGIGLTQSFALELAPFRIKVNSICPGNYYEGPLWSNPENGLFVQYLKAGKVPGAKT
                     IRDVEDYYLAQVPMRKGCSPLDVSKGVLYLMDQCGETGQALPITGGQVMLN"
     gene            2032..>3367
                     /locus_tag="PLI37_07335"
     CDS             2032..>3367
                     /locus_tag="PLI37_07335"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOQ58858.1"
                     /translation="MSKKVSDSRLPDRIFESSWEVCNKVGGIHTVLSTHAKQLKVLYG
                     EAICYIGPDLQNEVQKAEFNETSTPLDEWSAYARRHDKLSIRCGRWNIPGEPLVILVD
                     FKKYYAYKNHIYSQFWEWFGVDSIQAYGDYDESCMFAYACGEVIRSWERYHLEQDPAV
                     VAHFHEWTTGLGLLYVKKHLPCVATVFTTHATSIGRSISGNGKKLYKYIEGYQGDQMA
                     RELNMVAKHSVEKTAAFQADCFTTVSDITAAECAQLIGKAPDLVTPNGFEDDFVPRGA
                     VAIAKRKAARRLLLEVAESILGYSLNKNVLMLATSGRYEFRNKGLDVFIESLHRLRHD
                     RSLDRQILAYILVPGDVSAARQEVLQRLEALRKSKTTSSSAKQQSDLGNSPEAESAQP
                     GPIYHPYNTHWLNHIEQDKVLNTIKHLGFTNGPDEKVKIIFVPCYLHGHDGIF"
BASE COUNT          895 a          871 c          833 g          768 t
ORIGIN      
        1 tcgatcgcga caaactgcgg ctcctctccg aaaagtccta cagcgacgat accgacttgc
       61 gcgaggccga ggtcaaaagc gatctggaag ccgcctgtat taccaaaaac cggcgcccgt
      121 cggtagaaac ttccatgcac aatgccatca actatgcttt tgtggtgcat ttacatccta
      181 cggccgtcaa cgggctgatg tgttcccggc aagccgaaaa taagcttagg gaactgtttg
      241 gcgatgaaca tctctacatt ccttataccg atcccggcta tgtcctattc aaaaaagtcg
      301 aaggagccat tgctgccttc cgggcaaagc ggggcaaaga gccctcggtg atttggttgc
      361 agaaccacgg tatctttgtg gctgccgaca atatcgaaga aattcgtcgt acctacgatc
      421 atatcctggc tgtgctcgat aaagaagtaa aaacggccgc tcacagggct gctgccggct
      481 ccaaggccct gcaggcctat cttcagccgg aagaacgtcc tgttccgggc agagtgatcg
      541 aaattctgcc tgcgctccgt atgttggttt ccgaaacaga tgagcaaaaa catattcagc
      601 ttaaaacact caaaatccgc aacaacaagc tgatagagta ctattgtgag aaaaaagagc
      661 attatatata catcagccgt ccctttacgc ccgacggcat agtgtattgc aaatccaacc
      721 agttatatat agataaggcc aaaaccgaag atatactgga tgatgcgaaa aaaatcctgc
      781 cttttttcag gcaacatttc ggctatcaac ccaaggtgat tgccctccgg ggtttgggta
      841 tcgtggccgt aggcgacaac gcagcccaat gcgataccat cctcgatgtt ttcgaagact
      901 gcatcaagat tgccgccatc tccgaagcct ttggaggccc gcatcccatg actcaggaac
      961 aaatcgattt tatcgatcat tgggaagtcg aaaactatcg ccgcagcatc gccgccggta
     1021 ccctaaccgg cagagcccaa aacaaaacca tagtggttac cggagctgcc cagggctttg
     1081 gccggggtat tgccgatgaa ctcctgaagg agggggccaa tattgtggtg gccgacctca
     1141 acagcgaaac cggcctggcc accgtggatg aattcaacca aaaagcccgc agcaacaggg
     1201 ctgtgtttgt gcagaccgat gtaagcaagg tcgccagtat cgagcagctt gtgtaccaaa
     1261 gtatttgttg tttcggtggc atcgactgtt ttatcagcaa cgcgggtatc ctccgggccg
     1321 gcggtctgga agaaatgagc cccgaaaact tcgatttagt gaccaaaatc aactacaacg
     1381 cctattttta ttgcaccaag gtggtgagtc gcgtaatgaa attgcaaacc gcttacggct
     1441 cccccgatta ttacgccgac atcatccaga ttaattccaa atcgggactg agaggcagca
     1501 gagccaattt tgcctatgcc ggcggtaaat tcggaggtat aggccttaca cagtcttttg
     1561 ccctggaact ggctcccttc cgcatcaagg tcaactccat ctgtcccgga aattattacg
     1621 aaggtccgct ctggagcaat cccgaaaacg gcctctttgt gcaatacctc aaggcgggca
     1681 aagtaccggg cgccaaaacc attcgggatg tcgaggatta ctacctggct caggtgccca
     1741 tgcgtaaagg ttgttcacct ttggatgtca gcaagggagt gctgtatctg atggatcaat
     1801 gcggcgaaac gggtcaggcg cttcccatta cgggcgggca ggtaatgctg aattgatgca
     1861 gtggcttgag tatgtgccga atgccggaaa gtctgttttt ttgcggcatg tgagctcgcc
     1921 attttctgta taaaaattaa ggaaattgaa caatctgcgg gcggtatttc actcaaaata
     1981 tgaaatagat tgactacctt tgcggctttc aaaactgaac aggaaaaagg tatgtccaaa
     2041 aaggtaagcg atagtcggct tcccgacagg attttcgagt ccagttggga ggtttgcaac
     2101 aaagtaggag gtatccacac cgtcttgtcg acgcatgcaa aacaactcaa agtcctttac
     2161 ggggaagcga tttgctacat aggccccgat ttgcaaaacg aagtgcaaaa ggccgagttc
     2221 aacgaaactt ccactccttt ggacgaatgg agcgcttatg cccgtcggca cgataaattg
     2281 tccatacgtt gcgggcgctg gaacattccc ggtgaacccc tggtgatttt agtagatttt
     2341 aaaaaatact atgcctataa aaaccatatt tactcccagt tttgggaatg gtttggcgtt
     2401 gattccattc aggcttacgg cgattacgac gaatcctgca tgtttgctta tgcctgcggc
     2461 gaagtcatcc gaagctggga gcgttaccac ctggaacaag atcctgcagt agtagctcat
     2521 ttccatgaat ggaccaccgg gctgggtttg ctctacgtca aaaaacacct gccctgtgtg
     2581 gccaccgtat ttactaccca tgccacttcc ataggccgtt ccattagtgg caacgggaaa
     2641 aaactctata agtacataga gggctatcag ggcgatcaga tggcaaggga actcaatatg
     2701 gtcgccaaac attcagtcga aaaaacggca gcctttcaag ccgattgctt taccaccgtc
     2761 agcgacatca cagccgccga atgtgctcaa ctaatcggca aagcccccga tctggtcacc
     2821 cccaacggtt tcgaagacga ttttgtgccc cggggagccg tggccattgc caaacgcaaa
     2881 gcagcccgcc gacttttact cgaagtggcc gaaagcatac tgggttacag tctcaataaa
     2941 aacgtgttga tgctggctac cagcggacgt tacgagtttc gcaacaaggg gctggacgtc
     3001 tttatcgaaa gcttgcaccg gctgcgtcac gaccggagcc tcgaccggca gattctggct
     3061 tatatcctgg tgcccggcga tgtaagcgct gcccgtcagg aagtgctcca aaggttggag
     3121 gcgctcagaa agagcaaaac gacttcgtct tcggccaaac agcaatcaga tttgggaaat
     3181 tccccggagg cagagtcagc tcaacccggc cccatttatc atccctacaa cacgcactgg
     3241 ctcaaccaca ttgagcagga taaagtgctc aacaccatta aacatctggg ctttaccaac
     3301 ggtccggacg agaaagtcaa aattatcttt gtgccctgct acctgcacgg acacgacggc
     3361 atattca
//
LOCUS       DAOSTD010000132         3194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_27294_length_3194_cov_15.351386, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000132 DAOSTD010000000
VERSION     DAOSTD010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27294_length_3194_cov_15.351386"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLI37_07340"
     CDS             complement(<1..374)
                     /locus_tag="PLI37_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004319284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase"
                     /protein_id="HOQ58859.1"
                     /translation="MKKIFLIVAVSLFLPALQAQNDLVEWKVQTNKTVSDINPDMYGI
                     FFEDINFGADGGLYAELIKNRSFEFPQNLMGWKTFGKVELRSIDAPFERNPHYVRLSP
                     SGHGHKHTGLENEGFRGIGLKEG"
     gene            complement(427..>3194)
                     /locus_tag="PLI37_07345"
     CDS             complement(427..>3194)
                     /locus_tag="PLI37_07345"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOQ58860.1"
                     /translation="RPEYKPGTRWWWLGSAVDKENLDWNLHYLHQAGIGSVEITPIYG
                     VQGEEARDIPYLSPRWMEMLKHVQDQAARLEMIVDMNGGTGWPFGGPEIDPAYAASRQ
                     LIQRYELEGISVSPSAGSKSAPTQSINLEVKDNRQRPHAQLQALLFVSADGSREILPI
                     EGVKDNILELPAGKKGELIALYCGKTLQQVKRSAPGGEGLVMNHLSKEALDHYLKKFD
                     RAFEQSDAAWPSSFFNDSYEVYGADWSEQLLEEFMKRRSYDLRLYLPELLGEGDSEVI
                     ARVVCDYRETISDMLLENFTIPWTEWAHERGSQTRNQAHGSPGNLLDLYAAMDIPECE
                     SFGCTNFDLPNLRVDEEIRRNDGNPATLKYASSAAHVSGKNYTSSESMTWLTEHFRTS
                     LALIKPEMDQLFLNGINRVFYHGTPYTPKEAAWPGWLFYASILVNPNNTIFRDMPALN
                     DYITRVQSFMQEGKPDNELLLYLPIYDIWQNYRQSNYLTFVIHSISDKLPEFNGLVYK
                     LREAGYDMDYISDRQLLETGFADGSLQTPGAQYKAILVPHCRVIPPATLRHLLNLAHQ
                     GANVMFLGSLPQEVPGLHEAAERREELRQLWRETGLKNDPKNQQSVTYGRGMLTVASD
                     LTELMKVSGIEPEEMRSVQGLDYIRRRYEDGYVYFVAMQHNRLVDAWVPLAKPASSVM
                     FFDPLTGQKAPAPLRRGQRDQAEVYLQIKPGQSLIVRTFDQGNLPGATYPVYEPSTLN
                     YGCRKEQKNQEALPLLGNWSFEFTEGQPLIPGQFKMKGQPQAWTELQVEGADTYAGTG
                     VYKLEFKLPKADADDWLLDFGSLAESARIRVNGKDAGMVWCVPYEIRLGEFLIPGKKN
                     TIEIAVTNMPANLIADYDRRGVNWRIFKDINIVSVFYKPITFDVWPVQPSGLLQTPRL
                     IPLINKK"
BASE COUNT          771 a          821 c          783 g          819 t
ORIGIN      
        1 ccttctttga ggcctatacc cctgaaaccc tcgttttcta gcccggtatg tttgtgtccg
       61 tgccccgaag gagacaaacg aacataatga ggattgcgct caaagggagc atcgatgctc
      121 ctcagctcca ctttgccaaa ggttttccaa cccataaggt tttgaggaaa ttcgaaggaa
      181 cggtttttta tcaattcggc atacaagccc ccgtctgctc caaagttgat gtcttcgaag
      241 aaaatgccgt acatgtcggg gttaatatcg gaaaccgtct tgttagtctg cactttccac
      301 tctacaagat cattctgtgc ctgcagcgcc ggtaagaaca gcgaaacggc cactatcaaa
      361 aaaatttttt tcataaagaa acgattaaac gaattataca atttttactt cacgtcaaag
      421 ataaagctat tttttattta ttaaaggaat caatcgaggg gtctggagta atcccgaagg
      481 ctgcacaggc catacatcga aagtgatggg tttgtaaaat acgctcacta tgttgatgtc
      541 tttgaatatg cgccagttta caccccgccg gtcgtaatcg gcaataagat tggcaggcat
      601 gttggtaacg gcaatttcga tggtgttttt cttgccggga atcagaaact cgcccagtct
      661 gatctcgtag ggcacgcacc acaccattcc ggcatctttg ccgttcacgc gtatccttgc
      721 actttcggct aaggagccga aatcgagcaa ccagtcgtcg gcgtccgctt tgggtaactt
      781 gaattccagt ttataaaccc cggtaccggc ataggtgtcg gctccttcca cctgcagttc
      841 tgtccaggct tgaggctggc ctttcatttt gaattgtccg ggaataagag gctggccctc
      901 tgtaaattca aagctccagt tgcccagcag gggcagcgcc tcctgatttt tttgttcttt
      961 ccggcaaccg taattgagcg tacttggctc gtaaacggga taggttgctc ccggtaagtt
     1021 gccctgatca aaagtacgga cgatcaagga ttgcccgggt ttgatttgca aataaacctc
     1081 ggcctgatct ctctggcccc tgcgcagggg agccggggct ttttggccgg tcagaggatc
     1141 aaaaaacatc accgaagagg cgggcttggc caggggcacc caggcatcga ccaggcgatt
     1201 gtgctgcatg gccacaaaat aaacataacc gtcttcataa cggcgtctga tataatccag
     1261 cccctgaacg gatctcattt cttcgggctc gataccggaa actttcataa gctctgtcaa
     1321 atcggaagcc accgtcagca ttccccgacc gtaagtcacc gactgctgat ttttcggatc
     1381 gtttttcaga ccggtttctc tccataattg acgaagctcc tctcgccttt cggcggcctc
     1441 gtgcagaccc ggcacttctt gaggcaaact gcccaagaac ataacattag ctccctggtg
     1501 ggctaaattg agcaaatgac gtaaggtggc cggaggtatt acccgacaat gcggcaccaa
     1561 tattgcctta tattgcgcac cgggcgtttg caggctgcca tcggcaaatc ctgtctccaa
     1621 caattgtctg tcggaaatat aatccatatc gtaaccggcc tcccgcagct tataaaccaa
     1681 gccgttgaat tcgggcagtt tgtcggaaat actgtgaata acgaaggtta ggtaatttga
     1741 ctgacggtag ttttgccaaa tgtcgtaaat gggtaaatac aacaaaagtt cattgtcggg
     1801 tttgccttct tgcataaagg attgaactct ggttatgtag tcgttcaaag ccggcatatc
     1861 cctgaaaatg gtattgttgg ggttcaccag tatggaagca taaaacagcc agcccggcca
     1921 ggccgcctct ttgggcgtat atggcgtgcc gtggtaaaac acccggttga taccattcag
     1981 aaacaattgg tccatttcgg gcttgatcag ggccagcgaa gttctgaaat gttcggtcag
     2041 ccaggtcata ctttcggatg aagtgtagtt tttcccgctg acatgggcgg cagatgaagc
     2101 gtatttgagg gtggccggat tgccgtcgtt acggcgtatc tcttcgtcta cccgcaagtt
     2161 gggcaggtca aagttggtac aaccgaaaga ctcgcattcg ggaatatcca tggcggcata
     2221 gagatccagc agatttcccg gtgaaccgtg ggcctgattc cgggtttgac tgccgcgttc
     2281 atgagcccat tccgtccagg gaatagtgaa attttccagc aacatatccg aaatggtctc
     2341 cctgtagtcg caaacgaccc tggcaataac ttcagagtcg ccttcgccca gcaattccgg
     2401 cagatataag cgtaaatcgt aggatcttcg cttcataaat tcctccagta attgttccga
     2461 ccagtcggcc ccgtacactt cgtaggaatc gttaaagaaa ctgctgggcc aagcggcatc
     2521 ggattgttca aaagcccggt caaacttctt caagtaatga tcaagagcct ctttcgacag
     2581 gtgattcatg accaggcctt ctcctccggg agccgaacgc ttgacctgtt gcagggtctt
     2641 gccgcaataa agggcaatga gttcgccttt tttcccggcg ggcaattcta gtatattatc
     2701 ttttactccc tctatgggca gaatttctcg actaccgtcg gccgacacaa acaacaatgc
     2761 ctgcaattga gcatgaggcc gctgccggtt gtccttcact tcgagattaa tgctttgtgt
     2821 gggagcagat ttggaaccgg cagaaggaga aacggatata ccttcgagct cgtaacgctg
     2881 tatcagctga cgactggccg cataggcagg atctatttcg ggccctccga agggccagcc
     2941 ggtacctccg ttcatatcca ctatcatctc caggcgggcg gcctggtctt gcacatgctt
     3001 gagcatctcc atccagcggg gcgacagata gggaatatcg cgggcttctt cgccctgtac
     3061 accgtaaatg ggggtgattt ccacactgcc tatacctgcc tgatgcaaat agtgcaaatt
     3121 ccagtccaga ttctccttgt ccacggcgct gcccaaccac caccaacggg ttccgggctt
     3181 atattcggga cgaa
//
LOCUS       DAOSTD010000133         3167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_27607_length_3167_cov_12.824550, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000133 DAOSTD010000000
VERSION     DAOSTD010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27607_length_3167_cov_12.824550"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLI37_07350"
     CDS             <1..372
                     /locus_tag="PLI37_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOQ58861.1"
                     /translation="SRGVFAGKVLVHPNAQKTSAYQSNANLCNSSRARMNALPQLEIY
                     ADDVKCSHGSATGQIDENALFYMRSRGLSEQEARAMLKFAFVADVIEGIQLIPLKNRI
                     HNLVGKRFRKQMSACAGCDIC"
     gene            389..1615
                     /locus_tag="PLI37_07355"
     CDS             389..1615
                     /locus_tag="PLI37_07355"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017141013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOQ58862.1"
                     /translation="MESKFDVASIRKHFPILEQKVYGHPLVYLDNAATTQKPRSVVKK
                     IEEAYFSHNANVHRAVHLLSQQATEAHEAARVVVQQFINAAEPDEIVFTRGTTESINL
                     VAFSFGEAFCREGDEILISSMEHHSNIVPWQLLCERKKMTLKVIPIDDNGCLLVDEYE
                     KLLGPKVKLLAITHVSNVLGIENPIKQIIEKAHKQGVPVLVDGAQAVAHMPVDVRALD
                     ADFYTFSSHKVFGPTGVGVLYAKRSFLEQMPPYQGGGEMIDQVHFEKTTYNRIPFKFE
                     AGTPDFIGTAALAEALRFIQEIGLDKIADYEKQLVNYALQELSKVEGFQLLGNIPERH
                     AIIPFQLKGIHAYDVGLLLDKLGIAVRTGHHCAQPLMERLAVDSCVRASFAFYNTSSE
                     VDRLQAALKKIYKMFN"
     gene            1625..2041
                     /locus_tag="PLI37_07360"
     CDS             1625..2041
                     /locus_tag="PLI37_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOQ58863.1"
                     /translation="MSIDEVQDDIIQAFDEIEDWMDKYQLLIDMGNSLGPLDEQYKKP
                     QYLIEGCQSRVWIHAEYKEGKVFFEAESDTVIVKGLVYLLLKVLSGRTPDEILKTDLY
                     FIERIGLKEHLSPTRSNGFAAMLKQIRNYALAFKLS"
     gene            complement(2014..>3167)
                     /locus_tag="PLI37_07365"
     CDS             complement(2014..>3167)
                     /locus_tag="PLI37_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOQ58864.1"
                     /translation="TPVYVTLFWTLVLKTANRHNNDRARLFLGKFMFFACILYFSHFL
                     FFYPLPQVYIYFDALYQYASLMVYPLFYIYIRLLTVDKKFELKKHYKYIAVPNLLLIA
                     YLIGILFSPYEHYRRLIADYENSPWNFLKIVLTCMQLAFLIQVILCVTGNTILLKKYK
                     HSAGQYYSDIEDSRIYKAELLNLCIILTGISSFILGILGRDFFFAEPVAMGIASLVFS
                     TLLFAIGLLGNVQKVVNPSYETDSGVQPSASLATDQEMEHFKAEVSPKILRLFDEDKI
                     YLNSKLTIRDVAQIVGTNRTYVSNFINTHFNQNFCSFVNRYRLEELERTIRKHPEYTG
                     NTLAELCGFGSTDSLKRAVRTSFGMSYSEWRKSLSVQADKHQDNLNAKA"
BASE COUNT          911 a          637 c          766 g          853 t
ORIGIN      
        1 tcgagagggg tgtttgccgg aaaagtgctg gtgcatccca acgcgcaaaa aacttctgct
       61 tatcagtcca atgccaatct ctgcaattcg tcccgagcca ggatgaatgc tttgcctcag
      121 ctcgagattt acgccgatga tgtcaagtgc agccatggtt ccgccaccgg ccaaattgac
      181 gagaacgcct tgttttatat gcgatcgaga ggcctttcgg agcaggaagc ccgggcgatg
      241 cttaaatttg cctttgtggc agacgtgatt gaggggattc aactaattcc cttgaaaaac
      301 agaatccaca atctggtggg caagcgattt cgtaaacaga tgagcgcttg tgccggctgc
      361 gatatttgtt gagaataaaa ctgaggtcat ggaatcgaaa tttgatgtag cttccataag
      421 aaagcacttt cctattctgg agcaaaaagt ctacgggcat cctttggtct atctggataa
      481 cgcagccact acccaaaaac cgcgttccgt ggtaaaaaag atagaagagg cctacttcag
      541 ccataatgcg aatgttcacc gggcggtgca tttattgagc caacaggcta ccgaagctca
      601 cgaggcggcc agagttgtgg tgcagcaatt tatcaatgcg gcagaacccg atgaaattgt
      661 tttcaccagg ggaaccaccg aatctatcaa cctggtggcc ttttcctttg gcgaagcttt
      721 ttgccgggag ggtgatgaaa ttttgatttc aagcatggag caccactcaa acatcgttcc
      781 ctggcaactc ttgtgtgagc gtaaaaaaat gactcttaaa gtgattccca tagacgataa
      841 cggttgttta ttggtcgatg aatacgaaaa attattggga cccaaggtaa aattactggc
      901 cattactcat gtttccaatg tactgggaat cgagaatccc ataaagcaga tcattgaaaa
      961 agcccacaag caaggtgttc ctgtgctggt tgacggagct caggcagttg cccatatgcc
     1021 ggtagatgtt cgagctctgg acgccgactt ttataccttt tcgtcccaca aggtttttgg
     1081 gcccaccgga gtaggagttt tatacgcgaa acgctctttt ttggagcaaa tgcctcctta
     1141 ccaaggggga ggagaaatga ttgaccaagt tcattttgaa aaaacaacat acaatcggat
     1201 tccatttaaa ttcgaggccg gaacacccga ttttatcgga acagcggcct tggccgaggc
     1261 attacgtttt attcaggaga taggcttgga taagattgca gactacgaaa agcagttggt
     1321 gaactatgcc ttgcaggagc tgagcaaagt ggaaggtttt cagttgttgg gaaatatacc
     1381 tgagaggcat gccataattc cttttcaatt aaaaggcatt catgcgtatg atgttggctt
     1441 gttgctcgat aaactcggca ttgccgtacg caccgggcac cattgtgctc aaccgctgat
     1501 ggagcgtttg gcagtagata gctgtgttcg cgcctctttt gctttttaca atacaagctc
     1561 agaagtagat cgtttacagg ctgctttaaa gaaaatctac aaaatgttta attaacagat
     1621 tgaaatgagt atagacgaag ttcaggacga catcattcag gccttcgatg agattgaaga
     1681 ttggatggac aagtaccaat tattaatcga catgggcaat tctttaggtc cgctggatga
     1741 gcagtacaaa aaacctcaat acctgataga aggatgtcaa agccgggtat ggatacacgc
     1801 cgaatacaag gagggtaagg tgttttttga ggccgagagc gataccgtta ttgtaaaagg
     1861 cctggtttac ctgttgctga aggttctttc gggccggaca ccggacgaaa tactgaagac
     1921 cgacttatat tttattgaac gcattggact caaggagcat ttgtcgccca ccagatccaa
     1981 tggttttgcg gcgatgctca agcaaattcg caattacgcc ttggcgttca aattgtcctg
     2041 atgtttgtct gcttggacag acaaactttt tctccattct gaataagaca taccaaaact
     2101 agtccttaca gcccttttca gggagtccgt tgaaccgaaa ccgcagagtt cggccagggt
     2161 gttgccggtg tattccgggt gtttacggat ggttcgttct agctcttcga gtctgtaacg
     2221 atttacaaaa ctgcaaaagt tttgattaaa atgagtgtta ataaagttcg atacgtaagt
     2281 acggttcgtt ccgactattt gagccacatc tctgatggtg agtttgctgt ttagatagat
     2341 tttgtcttca tcaaacaagc gcagaatttt gggagaaacc tcagctttga agtgttccat
     2401 ttcctgatct gtggcaaggc ttgctgaagg ctggacgccg ctgtcggttt cataacttgg
     2461 attgactact ttctgcacat tgccgagtaa accaatagca aaaagcaagg tggaaaaaac
     2521 caaggaagca atgcccatag ctacaggctc ggcaaaaaag aaatcacgtc ccagtattcc
     2581 gagtataaaa gaggagatac ctgtaagaat aatgcacaga ttgagtaatt cggccttgta
     2641 gattctgctg tcctctatat cggaatagta ttgtccggcg ctgtgtttgt attttttcaa
     2701 taagatggtg ttgcctgtga cacaaagaat tacctgtatt aaaaaggcaa gttgcatgca
     2761 ggttaaaact attttcagga aattccaggg gctgttttca tagtcggcga tcagtctccg
     2821 gtagtgttca taagggctga acagtatgcc tatcaaatag gcgatcaaaa gcagattggg
     2881 tacggcgatg tacttgtaat gctttttaag ttcaaatttt ttatccactg tcaataggcg
     2941 aatgtagatg taaaaaaggg ggtataccat caaagaggca tattggtaaa gtgcgtcgaa
     3001 ataaatataa acctgaggta gaggatagaa aaaaagaaaa tgggaaaaat acaggataca
     3061 agcaaaaaac ataaatttgc ccagaaacag cctggctcga tcgttattgt gtctgtttgc
     3121 cgttttcaga actaaagtcc aaaacaaggt tacatacacg ggagtca
//
LOCUS       DAOSTD010000134         3137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_27967_length_3137_cov_15.364374, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000134 DAOSTD010000000
VERSION     DAOSTD010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27967_length_3137_cov_15.364374"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1800
                     /locus_tag="PLI37_07370"
     CDS             <1..1800
                     /locus_tag="PLI37_07370"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOQ58865.1"
                     /translation="AVEHCGSHNFGAFNLEKIIYQSSPNRRVTANLYLPGQAGKHPVV
                     LMLCGHGAQGKQTPFYQYQAALYAANGIATMVVDPPGQGERFQLIDKDGNNPVRGGSG
                     TNEHTLLLMNSLIVGESMVKDELWDNLRAIDYLCSRPDIDTSRIGCFGFSGGGTQAEY
                     LLAFDMRIKVGGIGGYFTQRRRSMEVIAPQDGCQHLPGEGLKALEQVDFVTAFAPKPL
                     IIINGAYDFVDRIGAEAGYRELKKFYAACGRPEQVLLYTGDDGHAFGEEKQRLVLAWF
                     LKYLCNKEQKGETFRDPSSEADYASVRAFAEETGITPAYDFEPIPADEMLCTPKGNLS
                     TYLPKGELLTPARNQQIWQEQAGSRAKFHTLSPEAQNRQVISLAGINPQVEVRVEKAG
                     STALEGIRFERYILHAENETPLPCLLLLPDKPQRDAVRIILHEQGKCLIADSCIQLCQ
                     NSGEIILLADLRGLGESRDKPERNESKYFNTEYSVGMMSLHIGKTLVGQRATDILMLA
                     SFIGQHETLKNCRDLQLEAYGKCVPPALHAAFLLDNLREIRLHSELPSWEYLLEHPTT
                     KEQLSYIVPKASKYYDLPDLQKALKARKISVIH"
     gene            1828..2586
                     /locus_tag="PLI37_07375"
     CDS             1828..2586
                     /locus_tag="PLI37_07375"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOQ58866.1"
                     /translation="MDIEKKRFKLSFYVPALLVFLLALVQALQSGLQESWYRLGIYPR
                     EISSLPGIITQVFIHENWAHFFNNAVPLFVLSWCFYYFYRELFWKGSLLLWISSGLFT
                     WLIGRANWHIGASGLIYAYSFFLFFSGLIREHKPLMAISFVVVFLYGSTFWNMFPWGE
                     FNRDISWEGHLSGAMAGLLWALLLRKQGPQAPPPPEEEDEEEAINSELDSTVEELAEK
                     NPDKEETKVTGENSSRQTTINVFPNAPEQQQPTA"
     gene            complement(2540..>3137)
                     /locus_tag="PLI37_07380"
     CDS             complement(2540..>3137)
                     /locus_tag="PLI37_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOQ58867.1"
                     /translation="MENILGRDRIIPEEKYGLKVLSIGFFVQKENAIVWRGPMASNAL
                     KQLIGDTYWGELDYFLIDLPPGTSDIHLTLVQNLKLSGAIVVTTPQQVALTDARKAVD
                     MFQNEKINVPILGLVENMSWFTPAELPQNRYYLFGKDGGAELAKERGVPLLGQIPLVQ
                     SVCESGDAGRPAALNADTVMGQAFRQLAAAVQERLEKR"
BASE COUNT          835 a          826 c          748 g          728 t
ORIGIN      
        1 gctgtggaac attgcggcag ccacaatttc ggagcattca acttagagaa aatcatctat
       61 caaagcagcc ccaaccgcag ggtcaccgcc aatctttatt tgcccggtca agccggaaaa
      121 caccctgtcg tgctgatgct ttgcggacac ggagcccaag gcaaacaaac gccgttttac
      181 caataccagg cagctttgta tgccgccaac ggcatagcca ccatggtagt agatcctccc
      241 ggtcagggcg aacgctttca gttgatcgac aaggacggca acaatccggt tcgtggcggc
      301 agcggcacca acgaacacac cctcttgctt atgaattcac tgatagtggg tgaatctatg
      361 gtcaaagacg aattatggga caacctgaga gccatcgact acctgtgcag ccggccggat
      421 atcgatacga gcagaatagg ctgtttcgga ttttcgggcg gcggcaccca ggcagaatac
      481 ctgcttgcct tcgatatgcg tattaaggtg ggaggcatcg gaggttattt tacgcaaaga
      541 cgaagaagca tggaagtgat tgctccccag gacggttgtc agcatttgcc cggcgaaggc
      601 ctcaaagctc tcgaacaggt ggactttgta acggcttttg cccctaaacc actgatcatt
      661 atcaacggag cctacgattt tgttgaccgt ataggagccg aagcaggcta cagggaatta
      721 aaaaaattct atgctgcctg cggacggccg gaacaggtac tcctatacac cggcgacgac
      781 ggccatgcct ttggagaaga aaaacaaagg ctggtactgg cctggttcct caagtacctc
      841 tgcaacaaag agcaaaaggg cgaaactttt cgcgaccctt cctctgaagc cgactatgct
      901 tccgtaaggg cttttgcaga ggaaaccggc ataacacctg cctacgactt tgaacccatc
      961 cctgccgacg aaatgctctg cacgcccaaa ggaaatttaa gcacctatct accgaaagga
     1021 gaactgctga caccggcacg caatcaacaa atctggcagg agcaggccgg atcaagagcc
     1081 aaatttcaca cactgagccc agaagcacaa aaccggcaag tcatctcctt agccgggata
     1141 aatccccagg tcgaagtcag agtggaaaaa gccggatcca ccgctttgga aggcataaga
     1201 tttgaaaggt atattctgca cgccgaaaac gaaacgcccc taccctgcct gttacttttg
     1261 cccgataaac cgcaacggga tgccgtcagg attattctgc acgagcaggg caaatgcctt
     1321 atagcagact cctgcattca actttgccaa aacagcggtg agattatatt actggccgac
     1381 ctcaggggct tgggcgaaag ccgcgataaa cccgaacgca acgaaagcaa atatttcaat
     1441 acggaataca gtgtaggcat gatgtcgctg cacatcggca agactttggt cggacaacgt
     1501 gccaccgata ttttaatgct ggcctccttt atcgggcaac acgaaacact caaaaattgc
     1561 cgcgacctgc agttggaagc ctacggaaaa tgcgttcctc ctgccctgca cgccgctttt
     1621 ttactggata acctccggga aatcaggctg cacagcgaac tgccttcgtg ggagtatcta
     1681 ctggaacatc ccacaaccaa agagcagctc tcctacattg tacccaaagc ttcaaaatac
     1741 tacgacctgc ccgatctgca gaaggctctg aaagcccgca agatatcggt tatacactga
     1801 acttaaggct ttaaattcca tcgcaatatg gacattgaaa aaaaacgctt caagctttct
     1861 ttttatgttc ccgctctgct ggtgttcctg ctcgccctgg tacaggccct gcaaagcggt
     1921 ttgcaggaaa gctggtacag gttaggcatc tatcctcgcg aaatctccag tcttccggga
     1981 ataatcacac aggtatttat ccacgaaaac tgggcacatt tttttaataa tgccgttccc
     2041 ctctttgttt tgagttggtg tttttactat ttctatcggg aactcttttg gaagggcagc
     2101 cttttgctgt ggattagctc cggactgttc acctggttga tcggtagagc caactggcat
     2161 atcggagcca gcggtttaat ttatgcctac tccttttttt tgttcttcag cggattgata
     2221 cgcgaacata aaccactgat ggccatatcg tttgtagtgg tattcctcta cggtagcacc
     2281 ttttggaata tgttcccctg gggggaattc aacagagaca tctcctggga aggtcatctg
     2341 tcgggagcca tggccggcct gctctgggcc ttgctgctaa gaaagcaggg gcctcaggcc
     2401 ccacctcccc cggaagaaga agacgaagaa gaagcgataa attccgaatt ggattctacg
     2461 gtagaagaac ttgctgaaaa gaatccggac aaggaggaaa ccaaagttac cggtgaaaat
     2521 tcaagccggc aaactaccat caacgttttt ccaaacgctc ctgaacagca gcagccaact
     2581 gcctgaaagc ctgtcccatt acagtatcgg cgttgagggc cgccggtctt cccgcatcgc
     2641 ccgattcaca gacgctttgc accaaaggta tctgccccag cagcggaact cctctttctt
     2701 tggccaactc tgctcccccg tctttaccaa acaaataata gcggttttga ggcagttcgg
     2761 ccggagtaaa ccaagacata ttttcgacca aacccaggat gggtacattg atcttttcgt
     2821 tttggaacat atcgaccgcc ttacgggcat cggtcagtgc cacctgttga ggggtggtaa
     2881 ccacaatggc tcccgaaagt ttcaaattct gtaccaaagt aagatgaata tcgctggtgc
     2941 ccgggggcaa gtctatgagg aaataatcca attcacccca ataggtatct cctatcaatt
     3001 gtttcagggc attggaggcc atggggccac gccagacgat ggcattctct ttttgtacaa
     3061 agaatcctat agataacacc ttgagcccgt atttttcttc gggaatgatt cggtcgcgtc
     3121 ccaaaatatt ctccatg
//
LOCUS       DAOSTD010000135         3107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_28302_length_3107_cov_13.080275, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000135 DAOSTD010000000
VERSION     DAOSTD010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28302_length_3107_cov_13.080275"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLI37_07385"
     CDS             complement(<1..277)
                     /locus_tag="PLI37_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOQ58868.1"
                     /translation="MRSFRSLFIRIPLYLFCLQLLSCASIGSPSGGPKDETPPVLLKS
                     LPENNSRNFTKKKIEIHFDELVAVENASEKVIVSPPQKTPPQVNAIMR"
     gene            404..1117
                     /gene="dapB"
                     /locus_tag="PLI37_07390"
     CDS             404..1117
                     /gene="dapB"
                     /locus_tag="PLI37_07390"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOQ58869.1"
                     /translation="MNIVLIGYGKMGREVERIALERGHSIACAIDVNNLELFESDDFR
                     NADLAIEFTGPSTALQNYRRCFAAGLPVVSGTTGWLEHMGEIRRACEQGQTFFYASNF
                     SLGVNLFFVLNRMLAEKMNAFPQYEVRLKEIHHVQKLDAPSGTAISLAEDILKNLDRK
                     KNWVNYESKESDELSIVSERIGSVPGYHEVCYESEVDSIKITHDAKNRAGLALGAVLA
                     AEYTLGKKGFLGMHDLLGF"
     gene            1129..2580
                     /gene="lepB"
                     /locus_tag="PLI37_07395"
     CDS             1129..2580
                     /gene="lepB"
                     /locus_tag="PLI37_07395"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOQ58870.1"
                     /translation="MSNTEKKCRFVATKRKWISFGLWTLVFILFVVWTGSLWWLLPIP
                     FAFDLFISRYIPWSFWKKSKNKTFRVIMDWVDAIVFALVAVYFVFLFFFQNYQIPSSS
                     LEKSLLVGDYLFVSKLSYGPRMPNTPLSFPLAQHTLPVVNSKSYFEKPQWPYKRIKGL
                     GKIQRKDIVVFNFPAGDTVALKMQNTDYYTICYSIARERGISMQEAARIVRTNPQTFG
                     EIVYRPVDRRENYVKRCVGLPGDSLQIISNQVYINGKILEDEPGTQYNYFVQTKGSYF
                     SEAQFRQWNISKEDRLLFPEQYSLPDLMGFEPDAQGNKLPVYHFPATKEVIEKIKKNP
                     IVNEVRIEPAQVGPYDLGGSIFTLSDEVSWTRDDFGPLWIPCKGATIELNPVNVLLYG
                     RVICAYERHSLEERQGLYFIDGEPAEHYTFEMDYYWMMGDNRHNSADSRAWGFVPEDH
                     VVGKPMRVWLSLDKDRNWFQGKIRWKRFMKKAV"
     gene            2588..>3107
                     /locus_tag="PLI37_07400"
     CDS             2588..>3107
                     /locus_tag="PLI37_07400"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOQ58871.1"
                     /translation="MSVVLLSTAYLAPVSYYVLLARAGKVVIEQHENYQKQSYRNRCL
                     IAAANGPMVLSIPVKKPLPGKNSIRDIQLSGHGNWQHMHWNSLVSAYASSPYFAFYRD
                     DIRPFYEKKYKYLFDFNEALREEICALLHLEPRLEYSKSYTTGPETEILDYRELIHPK
                     KQRLEELLPGPYV"
BASE COUNT          847 a          679 c          748 g          833 t
ORIGIN      
        1 ttctcatgat ggcattgact tgaggaggtg ttttttgcgg gggagaaaca atcacctttt
       61 cggaggcatt ctcaacagca accaactcgt caaaatggat ttcaatcttt tttttggtga
      121 aattccggct gttgttttcg ggcagacttt ttagcaaaac agggggtgtc tcgtctttgg
      181 ggcccccact tggcgatccg atgctggcac aggatagcaa ttgcagacaa aacagataga
      241 gtggaatacg aataaaaagg ctgcgaaaac ttctcatctg aaattagaac acgaggttgg
      301 aagatcttag agttcagggc aaatgtacga gtattttttc aatccgtctt ccggcattta
      361 aaaataatcc agtacctttg ccgtttgagt aaaacacggc attatgaata tagttttgat
      421 cggttacggt aaaatggggc gtgaggtaga acggatagct ttggaaagag gccatagtat
      481 tgcctgcgcc atagatgtaa acaacctgga actattcgaa tcggacgatt ttcgcaacgc
      541 cgaccttgcc atcgaattta ccggcccttc taccgccctg cagaattatc gtcgttgttt
      601 tgcggcaggt ttgcccgtgg tttcgggaac taccggctgg ctggagcata tgggcgaaat
      661 tcgccgggcc tgcgagcaag gacaaacttt tttttatgcc tccaatttta gcttgggagt
      721 caatttgttt ttcgtgctga acagaatgct ggccgaaaag atgaatgctt ttccgcaata
      781 tgaagtcaga ctgaaggaaa ttcatcacgt ccagaaactg gatgctccca gcggaacagc
      841 cataagcctg gcagaagata tcctgaaaaa ccttgaccgg aaaaaaaact gggtaaatta
      901 cgaaagtaag gaatccgacg agttgagtat tgtttccgaa cgtataggca gtgtgcccgg
      961 ttatcatgaa gtttgctacg agtcggaggt ggacagcatc aaaatcacac acgatgccaa
     1021 gaatagggcg ggattagccc tgggggcagt attagctgcc gaatacaccc tgggtaaaaa
     1081 aggctttttg ggcatgcacg atttactcgg attctaatca taaaaaatat gagcaataca
     1141 gaaaaaaaat gtcgatttgt cgctaccaaa cgcaagtgga taagttttgg cttgtggacc
     1201 ctggtcttta tcctttttgt ggtatggacg ggcagtttgt ggtggctttt gcccattccc
     1261 tttgcttttg atttgtttat cagccgctat attccctggt ctttctggaa aaaatccaag
     1321 aataagacct tcagggtaat aatggactgg gtagatgcca ttgtatttgc cttagtggcg
     1381 gtgtattttg tctttttgtt cttctttcaa aattatcaaa tacccagctc ttcgctcgaa
     1441 aaatcgctgt tggtgggcga ttatttattt gtcagcaagc tcagctacgg acctcgcatg
     1501 cccaacactc ccttgtcgtt tcctttggcc caacatactt tgcccgtagt aaacagcaaa
     1561 tcctattttg aaaagcccca gtggccttac aaacgcataa aaggcctggg caaaattcaa
     1621 cgcaaggata tagtggtgtt taatttcccc gccggcgaca cggttgccct gaaaatgcag
     1681 aacaccgatt attacaccat ttgctattcg attgcccgcg aaagaggtat aagtatgcaa
     1741 gaggcggccc gcattgtccg cacaaatccc caaactttcg gcgaaatagt gtaccgtcct
     1801 gtcgatcggc gcgaaaatta cgttaaacga tgcgtcggcc tgcctggaga cagtttgcaa
     1861 attatcagca atcaggtgta tatcaatgga aaaatcctgg aagacgagcc cggcacccaa
     1921 tacaattact ttgtgcaaac caagggctcc tatttcagtg aagctcagtt cagacaatgg
     1981 aacatcagca aagaagatcg tttgctcttt cctgaacagt attctttgcc ggatttgatg
     2041 ggttttgagc ccgatgctca aggcaacaaa ctaccggtgt accattttcc ggccaccaaa
     2101 gaggtgattg aaaaaataaa aaagaatccc attgtgaatg aggttcgtat agaacctgcc
     2161 caggttggtc cttacgatct gggcgggtcg atttttactt taagcgatga agtttcatgg
     2221 acccgcgatg atttcggtcc tctttggata ccctgcaagg gtgcgacgat agagctgaat
     2281 cctgttaatg tgctgcttta cggcagggtc atatgtgctt atgagcgtca tagccttgag
     2341 gaacgccaag gtctttattt tattgacgga gagccggcag agcactatac cttcgaaatg
     2401 gattattatt ggatgatggg cgacaaccgc cataattccg ccgattccag ggcctggggc
     2461 tttgtgcccg aagaccacgt ggtgggcaaa cccatgcgcg tatggttgtc gctcgacaaa
     2521 gatcgcaatt ggtttcaggg taaaatacgc tggaagcgtt ttatgaaaaa agccgtttaa
     2581 aaagcccatg tctgtagtct tgttatcgac ggcctacctg gcgccggtat cttattatgt
     2641 tttgctggca agagccggga aggtcgtgat agaacagcac gaaaactatc aaaagcaaag
     2701 ctaccgtaat cgctgcctga ttgctgccgc caacgggccc atggtgttga gcattcccgt
     2761 gaaaaaaccc ctacccggca aaaattcaat ccgcgacata caactgtccg ggcacgggaa
     2821 ttggcaacac atgcattgga acagccttgt ttcagcctat gcttccagcc cttattttgc
     2881 tttttaccgg gacgatatac ggccttttta cgaaaagaaa tacaagtatt tgttcgattt
     2941 taacgaggct ttgagagaag aaatctgtgc cttacttcat ttggagccca ggcttgaata
     3001 cagcaaaagc tatacaaccg gaccggaaac agaaatactt gattacaggg agcttataca
     3061 ccctaaaaaa caaagactgg aggaattgtt gcccggtcct tatgtac
//
LOCUS       DAOSTD010000136         3106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_28312_length_3106_cov_10.938053, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000136 DAOSTD010000000
VERSION     DAOSTD010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28312_length_3106_cov_10.938053"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLI37_07405"
     CDS             complement(<1..277)
                     /locus_tag="PLI37_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOQ58872.1"
                     /translation="MRSFRSLFIRIPLCLFCLQLLSCASIGSPSGGPKDETPPVLLKS
                     LPENNSLNFTKKKIEIHFDELVAVENASEKVIVSPPQKTPPQVNAIMR"
     gene            404..1117
                     /gene="dapB"
                     /locus_tag="PLI37_07410"
     CDS             404..1117
                     /gene="dapB"
                     /locus_tag="PLI37_07410"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOQ58873.1"
                     /translation="MNIVLIGYGKMGREVERIALERGHSIACAIDVNNLELFESDDFR
                     NADLAIEFTGPSTALQNYRRCFAAGLPVVSGTTGWLEHMGEIRRACEQGQTFFYASNF
                     SLGVNLFFVLNRMLAEKMNAFPQYEVRLKEIHHVQKLDAPSGTAISLAEDILKNLDRK
                     KTWVNYESKKSDELSIVSERVGSVPGYHEVCYESEVDCLKITHDAKNRAGLALGAVLA
                     AEFTLGKKGFLGMHDLLGF"
     gene            1128..2579
                     /locus_tag="PLI37_07415"
     CDS             1128..2579
                     /locus_tag="PLI37_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOQ58874.1"
                     /translation="MSNTEKKCRFVAAKGKWISFGLWTLVFIFFVVWTGSLWWLLPIP
                     FAFDLFISRYIPWSFWKKSKNKTFRVIMDWVDAIVFALVAVYFVFLFFFQNYQIPSSS
                     LEKSLLVGDYLFVSKLSYGPRMPNTPLSFPLAQHTLPIVNSKSYIEKPQWPYKRIKGL
                     GKVQRKDIVVFNFPAGDTVALKMQNTDFYSICYSIARERGISMQEAGRLVRTNPQTFG
                     DIVYRPVDRRENYVKRCVGLPGDSLQIISNQVYINGKILEDEPGTQYNYFVQTKGSYF
                     SEAQFRQWNISKEDRYLMPEQYPLPELLGFEPDAQGNKLPVYHFPATKEVIEKIKKNP
                     LVNEVRIEPAQVGPYDLGGSIFTLNDEVSWTRDDFGPLWIPCKGAKIELNPANVLLYG
                     RVIRAYERHSLEERQGRYFIDGEPAEHYTFEMDYYWMMGDNRHNSADSRAWGFVPEDH
                     VVGKPMRVWLSLDKDRNWFQGKIRWKRFMKKAV"
     gene            2587..>3106
                     /locus_tag="PLI37_07420"
     CDS             2587..>3106
                     /locus_tag="PLI37_07420"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOQ58875.1"
                     /translation="MSVVLLSTAYLAPVSYYVLLARAEKVIIEQHENYQKQSYRNRCL
                     IASANGPMVLSIPVKKSLPGKNSIRDIQLSGHGNWQHVHWNSLVSAYASSPYFAFYRD
                     DIRPFYEKKYKYLFDFNEALREEICALLHLEPRLEYSKSYTPGPETEILDYRELIHPK
                     KQRLEELLPGPYV"
BASE COUNT          844 a          693 c          744 g          825 t
ORIGIN      
        1 ttctcatgat ggcattgact tgaggaggtg ttttttgcgg gggagaaaca atcacttttt
       61 cggaggcgtt ctcaactgca accagctcgt caaaatggat ttctatcttt tttttggtaa
      121 aattgagact gttgttttcg ggcagacttt tcagcaagac agggggcgtc tcgtctttgg
      181 ggcctccgct tggcgatccg atactggcac aggacagcaa ttgcagacaa aacaggcaga
      241 gcggaatacg aataaaaagg ctgcgaaaac ttctcatctg aaattagcac acgaggttgg
      301 aagatattag agttcagggc aaatgtacga gtattttttc aatccgtctt ccggcattta
      361 aaaataatcc agtacctttg ccgtttgagt aaaacacggc attatgaata tagttttgat
      421 cggttacggt aaaatggggc gtgaggtaga acggatagct ttggaaagag gccatagtat
      481 tgcctgcgcc atagatgtaa acaacctgga actattcgaa tcggacgatt ttcgcaacgc
      541 cgaccttgcc atcgaattta ccggcccttc taccgccctg cagaattatc gtcgttgttt
      601 tgcggcaggt ttgcccgtgg tttcgggaac taccggctgg ctggagcata tgggcgaaat
      661 tcgccgggcc tgcgagcaag gacaaacttt tttttatgcc tccaatttta gcttgggagt
      721 caatttgttt ttcgtgctga acagaatgct ggccgaaaag atgaacgctt ttccgcagta
      781 cgaagtcaga ctcaaggaaa tacatcacgt ccagaaactg gatgctccca gcgggacagc
      841 cataagcctg gcagaagata tcctgaaaaa ccttgaccgg aaaaaaacct gggtaaatta
      901 cgaaagtaaa aaatccgacg agttgagtat tgtttccgaa cgcgtaggca gtgtgcccgg
      961 ttatcacgaa gtttgctacg agtcggaggt agattgcctc aaaatcacac acgatgccaa
     1021 gaacagggca ggattagccc tgggagccgt attggctgcc gaatttaccc tgggcaaaaa
     1081 aggctttttg ggcatgcacg atttactcgg attctaatca caaaaatatg agcaatacag
     1141 aaaaaaaatg tcgattcgtc gctgccaaag gtaagtggat aagttttggc ctgtggaccc
     1201 tggtctttat cttttttgtg gtatggacgg gcagtttgtg gtggcttttg cccatccctt
     1261 ttgcttttga tttgtttatc agccgctata ttccctggtc tttctggaaa aaatccaaga
     1321 ataagacctt cagggtaata atggactggg tagatgccat tgtatttgcc ttagtggcgg
     1381 tgtattttgt ctttttgttc ttctttcaaa attatcaaat acccagctct tcgctcgaaa
     1441 aatcgctgtt ggtgggcgat tatttatttg tcagcaagct cagctacgga cctcgcatgc
     1501 ccaatactcc cttgtcgttt cctttggccc agcatactct gcccatagta aacagcaaat
     1561 cctatattga aaagccccag tggccataca aacgtataaa aggcctgggc aaggttcaac
     1621 gcaaggatat agtggtattt aattttcccg ccggcgatac ggttgccctg aaaatgcaga
     1681 acaccgattt ttacagcatt tgttattcaa ttgcccgcga aagaggtata agtatgcaag
     1741 aggcagggcg ccttgtccgt actaatcccc aaacttttgg cgacatagtg taccgtcctg
     1801 tcgatcggcg cgaaaattac gttaaacgat gcgtcggtct gcccggagac agtttgcaaa
     1861 tcatcagcaa tcaagtgtat atcaatggca aaatcctgga ggacgaaccc ggcacccaat
     1921 acaattactt tgtgcaaacc aagggctcct atttcagcga agctcagttc aggcaatgga
     1981 atatcagcaa agaagaccgc taccttatgc ccgaacaata tcccttgccc gaattgctgg
     2041 gtttcgagcc cgatgctcaa ggcaacaaac taccggtgta tcattttcct gccaccaaag
     2101 aggtgatcga aaaaatcaaa aagaatcccc ttgtgaatga ggttcgtata gagccggcgc
     2161 aagtcggccc ttacgatctg ggcggatcga tttttacttt aaacgatgag gtttcgtgga
     2221 cccgcgatga tttcggccct ctgtggatac cctgcaaagg agcgaaaata gagctgaatc
     2281 ctgctaatgt gctgctttac ggcagggtca tacgtgctta tgagcgtcac agcctcgaag
     2341 aacgtcaggg tcgctatttt attgacggag agcccgcaga gcattatact tttgaaatgg
     2401 attattattg gatgatgggc gacaaccgtc ataattccgc cgactcgagg gcctggggtt
     2461 ttgtgcccga agaccacgtg gtgggtaaac ccatgcgcgt atggttgtcg ctcgacaaag
     2521 atcgcaattg gtttcagggt aaaatacgct ggaagcgttt tatgaaaaaa gccgtttaaa
     2581 aagcccatgt ctgtagtctt gttatcgacg gcctacctgg cgccggtatc ttattatgtt
     2641 ttgctggcaa gagccgaaaa ggtcatcata gaacagcacg aaaactatca aaagcaaagc
     2701 taccgtaatc gttgcctgat tgcttccgcc aacgggccca tggtgttgag tattcccgtg
     2761 aaaaaatcct tacccggcaa aaattcaatc cgcgacatac aactctccgg gcacgggaat
     2821 tggcaacatg tgcattggaa cagccttgtt tcggcctatg cttccagccc ttattttgct
     2881 ttttaccggg acgatatccg ccctttttac gaaaaaaaat acaagtattt gtttgatttt
     2941 aacgaagctt tgagagaaga aatctgtgct ttacttcatt tggagcccag gcttgaatac
     3001 agcaaaagct acacacccgg accggaaaca gaaatactcg attacaggga acttatacac
     3061 cctaaaaaac aaagactgga ggaattgttg cccggtcctt atgtac
//
LOCUS       DAOSTD010000137         3020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_29451_length_3020_cov_14.260708, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000137 DAOSTD010000000
VERSION     DAOSTD010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29451_length_3020_cov_14.260708"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLI37_07425"
     CDS             <1..255
                     /locus_tag="PLI37_07425"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOQ58876.1"
                     /translation="SKRISYIGKDDFFAYKLEYYDTNGELHRINILKDYRELGKGKYF
                     AFYMEMQNVKNNRRSVMSVDKFQMGSAMPEAQFSPTMIEQ"
     gene            complement(303..1124)
                     /locus_tag="PLI37_07430"
     CDS             complement(303..1124)
                     /locus_tag="PLI37_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58877.1"
                     /translation="MKQLLYSLVLLLGISACNCDCREQLPKPAISTPPAELQLDEFYK
                     KYINVNGIHLISSHRVPDSALYAAYRTLDAMTGMLPPEVLKAMTDINTRVGVMARYEG
                     TTDIPEHAHLANDKRLNWDVRARGLGGTLRSPLTTCAEENILCYQIDKYHAEDILVHE
                     FAHSIHLIGIISIDPGFNDRLQQALDAAIAEGKWNNTYAQTNISEYWAEGVQSWFNVN
                     AEVEYPDGKHNHVNTRSDLKKYDPRLYAILAEFFPESKEQISCHKHINLYRREKR"
     gene            complement(1136..>1339)
                     /locus_tag="PLI37_07435"
     CDS             complement(1136..>1339)
                     /locus_tag="PLI37_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOQ58878.1"
                     /translation="LDDNVDPASTMQVVDALIKANKDFELVVLPGVGHTMGGSYGEHK
                     RFDFFVRELLNVVPPKWNELKTD"
     assembly_gap    1340..1439
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1440..>3020)
                     /locus_tag="PLI37_07440"
     CDS             complement(<1440..>3020)
                     /locus_tag="PLI37_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOQ58879.1"
                     /translation="PEGREYVLVDAGDKNSKPLFDHQTLAGKLGQATGEQVEALKLQL
                     ERLRVNDELNQLSFGFKGKNWVYYLEQDSLQGETASMERPGRTGSRTHWMVTDEERDR
                     GAVNSPDNKYQAYIKDHNVYVRELPGGKEIALSIDGCVGNYYSSYIQWSPDSRMLATT
                     KIRPVEKRYIHMVESSPSDQLQPKLRTLEYTKPGDELRFRVPCIFKVETQEALIPSTE
                     LFNNQYQLSGLRWNKDNSGITFEYNERAHKVYRVLEMSAQNGLVRSLIEETAETFVNY
                     SRRFRHDLKNDEEIIWMSERDNWNHLYLYDRKNASVKNQITRGEWYVRDIIRVDEDAR
                     RIYFTANGVPKKGDPYLVRYYYINMDGSGLTCLTPDEGHHRATFSRDMKYLVDVYSRV
                     DLAPVAQLRDAESGKLIMPLEKADISELEKTGWKAPEVFVAKGRDGKTDIWGIIIRPS
                     NFDPNKKYPIIEYIYSGPGSHYVPKSFAPYYSDMTNLAELGFIVVKMDGMGTSFRSKA
                     FEEICYQNLKDAGFPDRIL"
BASE COUNT          630 a          686 c          790 g          814 t
ORIGIN      
        1 agcaagagga tttcctatat cggtaaggac gacttttttg cctataaact ggaatattac
       61 gacacaaacg gtgaacttca ccggatcaat atcctcaaag attaccggga attgggtaag
      121 ggcaaatact ttgcctttta catggaaatg caaaacgtaa agaacaatcg ccgctcggtg
      181 atgagcgtgg ataagtttca aatgggctct gctatgcctg aagcacagtt ttcaccaaca
      241 atgatcgaac aataagccgg tgtcttagaa gcacccaact gcggcgcttt caatattcgg
      301 gctcaacgtt tttccctgcg gtaaaggttg atgtgcttgt ggcaggatat ttgttccttg
      361 ctttcgggga aaaactcagc caaaatagcg tacaggcgcg gatcgtactt tttgagatcc
      421 gatcttgtgt tgacgtggtt gtgtttgccg tcgggatatt ccacttcggc gttgacattg
      481 aaccagcttt gtacaccctc ggcccagtat tctgagatat tggtctgagc gtaggtattg
      541 ttccacttac cctcggcaat ggcggcatcc agcgcttgtt gcaatctgtc gttgaagccc
      601 gggtctatgc ttataattcc gatcagatgg atggaatggg caaattcgtg aaccaggatg
      661 tcttcggcgt ggtatttatc aatttgatag cagagtatgt tttcttcggc acaggtggtg
      721 agcgggcttc ttaacgtacc gcccaggccc cgggccctga catcccagtt gagccgtttg
      781 tcgttggcca gatgtgcgtg ttcgggtata tcggtggtgc cttcgtaacg ggccataaca
      841 cctacccgcg tattgatgtc ggtcatagct ttgagcactt ccgggggcaa catgcccgtc
      901 atggcatcca gggtgcggta agccgcatag agcgccgagt cgggcacccg gtgcgaagaa
      961 atcaggtgga taccgtttac gttgatgtat tttttgtaaa attcatcgag ttgcaattct
     1021 gccggagggg tactgatggc aggtttgggg agttgttccc ggcaatcgca attacaggca
     1081 gatatgccca gcagcaaaac aaggctgtac aatagttgtt tcataggtat attttttagt
     1141 cggttttgag ttcattccat ttgggaggaa ccacattgag cagttccctt acaaagaagt
     1201 cgaagcgttt gtgctcgccg taagagccac ccatggtgtg acccacgccg ggcaatacga
     1261 ccagctcaaa atctttgttg gcttttatca gggcgtccac cacctgcatg gtcgaggccg
     1321 gatcgacgtt gtcgtcgagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     1441 gtatgcgatc ggggaagccg gcgtctttca ggttttgata acagatttct tcgaaggcct
     1501 tggagcggaa agaggtgccc atgccgtcca tttttactac aataaagccc agctcagcca
     1561 ggttggtcat atcggagtag tagggagcaa aagatttcgg cacatagtga ctgccggggc
     1621 cggaataaat gtattctatg atggggtatt tcttgttggg atcgaagttg gaaggtcgga
     1681 tgatgatgcc ccagatgtcg gttttaccgt cgcggccttt ggcaacaaag acctcgggcg
     1741 ctttccagcc ggttttttcc agttcgctga tatcggcctt ttccaagggc atgattaatt
     1801 tgccgctttc ggcgtcgcgc agttgagcta ccggggctaa atcaacgcgg gaatacacat
     1861 ccacgaggta tttcatatcc cgcgaaaaag tagcccggtg atggccttcg tcgggagtaa
     1921 gacaggtcag tccgctgccg tccatgttga tgtagtaata cctgacaagg taaggatctc
     1981 cttttttggg tacgccgttg gcggtgaaat agatgcgccg ggcatcttcg tccactctga
     2041 tgatgtcgcg cacataccat tctcccctgg tgatttgatt tttaacgcta gcgtttttgc
     2101 ggtcgtagag gtagaggtgg ttccaattgt cgcgttcgct catccatatg atttcttcgt
     2161 cgtttttcag atcgtggcgg aaacggcgcg aataatttac gaaggtttcg gctgtttctt
     2221 cgatcaggct gcgtacaaga ccattttgag cagacatttc cagcacacga tagactttgt
     2281 gcgcccgttc gttgtattcg aaggtaatgc cgctgttgtc tttgttccag cgcaaacccg
     2341 ataattgata ttgattgttg aacaattccg tcgagggaat aagggcttct tgggtttcga
     2401 ctttgaagat gcagggcacc cggaaacgca gctcatcacc gggcttggta tattccaaag
     2461 tccggagttt gggttgcaac tgatcggagg gcgacgattc caccatgtga atgtaacgtt
     2521 tctccaccgg cctgattttg gtggtggcca gcatgcggga atcgggcgac cattggatgt
     2581 aggaggaata atagtttccg acacagccgt cgatgctcag ggcaatctct ttgcctccgg
     2641 gtaattccct cacatatacg ttgtggtctt ttatgtaagc ctggtacttg ttgtccggag
     2701 agtttaccgc cccccggtcg cgctcttcgt cggttaccat ccagtgggtg cggctaccgg
     2761 tccgaccggg acgttccata ctggcggttt ctccctgcaa gctgtcctgt tccaggtagt
     2821 aaacccagtt ctttcccttg aagccgaacg atagttgatt taattcgtcg tttaccctca
     2881 gcctttcgag ctgaagtttt agggcctcga cttgctcgcc cgtggcctgg ccgagtttgc
     2941 ctgccaaggt ttggtgatca aaaagcggct tgctgttttt gtctcccgca tcgaccaata
     3001 cgtattcccg tccttcggga
//
LOCUS       DAOSTD010000138         2850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_31819_length_2850_cov_14.886583, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000138 DAOSTD010000000
VERSION     DAOSTD010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31819_length_2850_cov_14.886583"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1701
                     /locus_tag="PLI37_07445"
     CDS             <1..1701
                     /locus_tag="PLI37_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ58880.1"
                     /translation="ALSYDGLPVTDVQNGQIDLGRFALDALESISLSTGQTEDIFQPA
                     RLFASAGLLQLQSLKPGFAPGKKQNLRLKLQGGSFGLLNPSLLLQRKLNDRHSLSLDV
                     YYLQSRGDYPYLMYYGSEGDLVSRERRRNSDIRQFRLEPSWYWRNTKGSEAAVKFYYN
                     QSERGLPGAGIYYNTETSSGQRLWDKQSFIQGHYKSLLHPSWSLRLNTKINGSYTHYL
                     DPSFLNSDGRDEAEYRQMESYLSGALQYRPQSAFSLVFSQDISMQELFSEEFLVNRPQ
                     RLSLYTVLAGKATAGAWNLSGNLLFCRIMEEAGAGPQGADYSKLTPSVSVSLRLVQGW
                     YVRAFYKSIFRMPSFNDLYYGRIGNPDLIPENTRQLNLGTGLSTKLLPGQELSLSLDV
                     YTNRVENKIVAYPTQNVFIWTMLNLGEVSIKGADFSLDWACSLGKNADLALRASYTLQ
                     SVLNVTDPESRAYGHQLPYTPKHSGSGVLLLRAFDWQFSYNLLYSGIRYSGFENYALN
                     RMPAYADQGISLNKSFNLAKTKMTLGAEVLNLTGKQYEIIRNYPMPGRSFRAKLVFSR
                     "
     gene            1751..2827
                     /locus_tag="PLI37_07450"
     CDS             1751..2827
                     /locus_tag="PLI37_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58881.1"
                     /translation="MGLLLLTVLLTFSCEMEDRPETTGDLPGLDQGACRGLWILSEGL
                     MNLNNSTLAYYSFEQKSLNKNVFRSLNKRGLGDTGNDMKAYGSKLYIVVSLSSQIEVL
                     DLYSGLSIKRIPLFDENRQARQPRYIDFLDGKAYVCSFDGSLARIDTASLEVEAYVNC
                     GRNPDGICLANNKIYVSNSGGLDNPYFDNSVSVVDPLSFREIKKIETGLNPYRMQVDS
                     QGDIYVVLRGNLLDIPFSLCRIDSRLDSVVQVFNDIPAWNLAIHNDTAYIYHYSVQGG
                     ENSVKVFDCLTETIISDQFISDGTPITRPYAIEVNPYSGDVYISDAYDYTLWGDCLCF
                     SAEGNLKFRFESVGLNPSSLVFVK"
BASE COUNT          709 a          642 c          716 g          783 t
ORIGIN      
        1 gccctttcct acgatggctt gccggtgacg gatgtgcaaa acgggcagat agatttggga
       61 cgttttgccc tggatgctct tgaaagtata agcctgagta ccggacaaac cgaagatata
      121 tttcagcctg cccgtttgtt tgcttcagcc ggccttttgc aacttcaaag cctgaaaccc
      181 ggttttgctc ccggtaaaaa acaaaacctg aggctgaaac ttcagggcgg ttctttcggc
      241 ttgttgaatc cctctttgtt gctgcagcgt aagcttaatg atcggcactc tctcagcctg
      301 gatgtttact atttgcaatc ccggggagat tacccttact taatgtacta tggttccgaa
      361 ggggacctgg tcagcagaga acgtcgccgg aattcggata tccgccaatt caggctggag
      421 ccttcctggt attggcgcaa caccaaaggt tccgaggccg cggtcaaatt ctattacaat
      481 caatcggagc ggggtttgcc cggagccggt atctattaca atacggaaac ctcttctggt
      541 cagaggttat gggataagca gagttttata caggggcatt acaaaagcct tttgcatccg
      601 agttggagtc tgcgattgaa taccaaaata aacggcagtt acacccacta cctcgatccg
      661 tcctttttga attccgacgg acgggacgaa gcagagtacc ggcagatgga gtcctacctg
      721 agcggagctc tgcagtatag gccccaatcg gcattcagcc tggtcttttc gcaagatatc
      781 agcatgcagg agcttttttc agaagagttt cttgtaaacc gtcctcagcg cctgagttta
      841 tacaccgtgt tggccgggaa agctacggca ggcgcctgga atttgagcgg caatctgctt
      901 ttttgccgga ttatggaaga agcgggagcg gggcctcagg gagcggatta cagcaaactt
      961 acaccttcgg tttcggtgtc gttgaggctt gtacagggct ggtacgtgag ggctttttat
     1021 aaatccatat tcagaatgcc cagtttcaac gatttgtatt acggacgtat cggtaatccc
     1081 gatctgattc ccgaaaacac ccggcagctt aacctgggta ccggcctgag tacaaaactc
     1141 ttacctggac aggagcttag ccttagcctg gatgtgtata ccaaccgggt agaaaacaaa
     1201 atcgtggcct atcctacgca aaatgttttt atatggacca tgttgaatct gggagaagtc
     1261 agcataaaag gcgcggattt tagtctggat tgggcttgct ctttgggtaa aaacgcagac
     1321 ctagcgctta gagcaagcta taccctgcag tcggttttga atgtgaccga tcccgaaagc
     1381 agggcctacg gacatcaatt gccttataca cccaaacaca gcggatcggg cgttttgctt
     1441 ctaagggcct ttgactggca attctcgtat aacttgcttt attcgggcat tcgctattcg
     1501 ggcttcgaaa attatgcctt gaatcgtatg ccggcctacg ccgatcaggg aatctcgctc
     1561 aacaagtctt ttaatttggc taagaccaaa atgacgctgg gcgctgaagt gctcaattta
     1621 acaggaaaac agtatgaaat cattcgaaat taccccatgc cgggacgttc tttcagggcg
     1681 aagcttgttt ttagtcggta aacaaagatt tctgcctgct gttttggcat tagttacaag
     1741 acttgcagtt ttgggcttgc tcttgctgac tgttttgctg accttttctt gtgaaatgga
     1801 agaccggccc gaaaccaccg gcgatttgcc gggcctggac cagggtgctt gtcgtgggct
     1861 ttggattttg tccgaaggcc tgatgaattt aaataattct accctggctt attattcgtt
     1921 tgaacagaaa agcttgaata aaaatgtgtt cagatcgttg aacaaaagag gtttgggcga
     1981 taccggcaac gatatgaagg cctacggatc caaactgtat attgtagtga gcttgtcttc
     2041 gcaaattgag gtgctggacc tctacagcgg attgtcaata aagcgcattc ctttatttga
     2101 cgaaaaccgg caggccaggc agcctcgcta cattgatttt ctggacggta aagcttatgt
     2161 ctgttctttc gacggcagct tagccagaat cgatactgcc agcctcgaag tagaagctta
     2221 tgtgaactgc ggacggaatc cggacggaat ctgcctggca aacaataaaa tttatgtgtc
     2281 caattccggc ggtctggata atccctattt cgacaacagc gtttcggtgg tcgatccgct
     2341 cagctttcgt gaaataaaaa aaatagaaac agggctgaat ccataccgga tgcaggtaga
     2401 cagccagggc gatatttacg tggtgctccg gggtaatttg ttggatattc cttttagttt
     2461 atgccggata gatagccggt tggattcggt tgtgcaggtc tttaatgaca ttccggcctg
     2521 gaatctggct atacacaacg acactgccta tatctaccat tattcggtac aaggcggaga
     2581 aaactctgta aaggtctttg attgtctgac agaaacaata atttcggatc aattcatatc
     2641 ggatggaact cctatcacac gcccctatgc catagaggta aatccctatt ccggcgatgt
     2701 gtacatcagc gatgcctacg actatactct ttggggagat tgtttgtgtt tttctgcaga
     2761 aggaaattta aagtttcgat tcgagagcgt tggcttgaat cccagcagtt tggtttttgt
     2821 caaataaacc gcggactgct gttctgctcg
//
LOCUS       DAOSTD010000139         2830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_32107_length_2830_cov_14.145586, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000139 DAOSTD010000000
VERSION     DAOSTD010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32107_length_2830_cov_14.145586"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..854
                     /locus_tag="PLI37_07455"
     CDS             147..854
                     /locus_tag="PLI37_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOQ58882.1"
                     /translation="MVCNSGVSRYNNWYSFEKPEALYARKFLTKSIAPVATAALGIGI
                     LGMSDFELQLQESLNWNKDLKVPMYDDQLRYAPIAIGVILPLFGKYPKHKFLHLIPLL
                     SSAYLLADGVVHQLKAYTAIARPNGAREHDAFPSQHSSMAFVAATLLHYEFGDYSPWI
                     SIGGYTLAGWVAYARVAQNHHWTSDVLTGAAIGTLSTHIVYLSYNFLSDLFTKNNLTI
                     CPYPVEGGGGVYLSYSF"
     gene            945..1496
                     /locus_tag="PLI37_07460"
     CDS             945..1496
                     /locus_tag="PLI37_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOQ58883.1"
                     /translation="MGKLDAYKIPLDSLSIGTHVFEFQLDTPWFELMDGPDVQKGKVK
                     AIVRVINTGRLYDLRFELTGVVEVPCDRCLDDVTIEVDQKSQLFVKLGPGFYEESEDM
                     IVIPEKEGALNVAWFLYELIVLGIPMRHVHAPGKCNKAMMSKLKEHLTGPGDEDEIEP
                     DLPDGEEFEAIDDDQALGSDWED"
     gene            1553..1735
                     /gene="rpmF"
                     /locus_tag="PLI37_07465"
     CDS             1553..1735
                     /gene="rpmF"
                     /locus_tag="PLI37_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOQ58884.1"
                     /translation="MAHPKRKHSNTRTAKRRTHYKATLPTLAVCPNCGAQYIFHTVCG
                     ECGYYRGKLAIEQKES"
     gene            1871..2752
                     /gene="era"
                     /locus_tag="PLI37_07470"
     CDS             1871..2752
                     /gene="era"
                     /locus_tag="PLI37_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOQ58885.1"
                     /translation="MHKSGFVNIVGNPNVGKSSLMNVLVGEKMSIITSKAQTTRHRII
                     GIVNGPDYQIVFSDTPGVLKPKYKLQESMLNFSSSALVDADILLYMTDTVEKIEKNES
                     FLEKVKQATVPVLAIINKIDLSNEEALQKQVQALQAILPSAEVIPLSVLHKFGLDYLM
                     KRILALLPESPPYFGKDALTDRPARFFVSEIIREKILLYYQQEIPYAVEVVVESYKEE
                     KEKIYIAALILVERESQKGIIIGHQGKALKKLGTTARREIERFTGKNVFLELFVKVEK
                     DWRTNENSLKNFGYIQD"
     gene            2763..>2830
                     /locus_tag="PLI37_07475"
     CDS             2763..>2830
                     /locus_tag="PLI37_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOQ58886.1"
                     /translation="MGNIVAIVGRPNVGKSTLFNRLV"
BASE COUNT          820 a          571 c          676 g          763 t
ORIGIN      
        1 cttgcctgtt gtgtttatcg catttttgct ttgcgcacat tgtcgttgat gaaactgaca
       61 gcccgttgtc cgagacgggt ttttgtccgg aattgccggt aggctcacag gatagtacag
      121 cgttgcccga aaaaactaaa acgactgtgg tttgcaattc aggtgtgagt cgatacaata
      181 actggtattc ctttgaaaag ccggaggctt tgtacgccag gaaatttttg accaaaagca
      241 ttgctcctgt agcgacggca gcgcttggga ttggaatact cggaatgagt gatttcgagt
      301 tgcaactgca ggaaagcctg aactggaaca aagacttgaa agtacccatg tatgacgacc
      361 agttacgtta tgcacccata gccatcggag ttattctccc tttgttcggt aaatacccaa
      421 agcataaatt cttgcatctg attccattgc tgagttctgc ctatttgctg gccgacggag
      481 tagtacatca actgaaggca tatacggcaa tagcacgtcc taatggtgcc cgggaacacg
      541 atgcttttcc atcgcagcac agttccatgg cctttgtggc tgctacgcta ctgcattatg
      601 aatttggaga ttacagtcca tggatttcca ttggaggtta taccctggcc ggctgggtgg
      661 catatgcaag agtggctcaa aatcaccatt ggacgagcga tgtgctcaca ggcgccgcca
      721 tcggcacgct cagcacacac atagtctatc tgagctacaa cttcctttcg gatcttttta
      781 ccaaaaacaa tctgacaatc tgtccttatc cggtcgaagg cggaggcggt gtttatctgt
      841 cttattcatt ttaagccgaa cccgaagaga acaaaaattc atataaatat tgatttgtct
      901 gatttttttt ctacctttgc gcggtttttt agtaagtatt gtaggtgggt aagctcgatg
      961 catataagat tccgttggac agcctgagca ttggaactca tgtttttgaa tttcaactgg
     1021 atactccctg gttcgaatta atggacggac cggatgtgca aaaaggcaaa gtgaaagcca
     1081 tagttcgggt aatcaacacg ggccgcttgt atgatttacg ttttgagctg accggtgtgg
     1141 tagaggtacc ctgcgacaga tgcctggacg atgtgactat agaagtggat caaaaaagcc
     1201 agctttttgt caaactcggc ccggggtttt acgaagaaag cgaagacatg atcgttattc
     1261 ccgaaaagga aggagcactc aacgttgcat ggtttctata cgaattgata gttctcggta
     1321 tacctatgag gcatgtgcat gctccgggca aatgcaacaa ggcaatgatg agtaagctca
     1381 aggaacatct gaccggtccc ggcgacgaag acgagatcga accggatttg cccgacgggg
     1441 aagaatttga agccatagac gacgatcagg ctttgggatc tgactgggaa gactagaaaa
     1501 tggaatacaa ataatactaa tttataatat ataatatata atatatactc aaatggcaca
     1561 ccctaagcgc aaacattcga ataccagaac tgccaaaagg agaacgcatt acaaggcaac
     1621 acttcccaca ttggccgttt gtcccaattg tggcgcccag tatattttcc atacggtttg
     1681 tggagaatgc ggatattatc gcgggaaatt ggcaattgaa caaaaagagt cctgattgaa
     1741 ggtttttttc gggaatgatt tcttcccccg gtagtgatgc cgcggccgaa gatctggtat
     1801 aacggatgtc acaatatata tggtgttacg tatataaagt gaatgtccgt tttttcattt
     1861 atattttaat atgcataaat ccggttttgt aaatattgtc gggaatccca atgtagggaa
     1921 gtcgagcctg atgaatgtcc tggtggggga aaagatgtcc attattacat ccaaagctca
     1981 aaccacccgc cacaggataa tcggaattgt caacgggccg gattatcaga ttgttttctc
     2041 cgataccccc ggcgttctga agcctaaata caaattacag gaaagcatgc tgaatttttc
     2101 ttcgtcggct ctggtagacg cggatatact tttgtatatg accgataccg tcgagaagat
     2161 cgaaaaaaac gaaagttttc tcgaaaaagt aaaacaggca acggtacctg tgctggctat
     2221 aattaacaaa atagatctga gcaacgaaga agctttgcaa aaacaagttc aagctttgca
     2281 ggctattttg ccaagtgccg aagtgattcc cttatcggtg ctgcataaat tcggtttgga
     2341 ctatttaatg aaacgtatat tggctttatt gcccgaatcg ccgccctatt tcggcaaaga
     2401 tgccctgaca gaccggccgg cacgtttttt cgtgtcggaa atcatacgcg aaaaaattct
     2461 gctttattat caacaagaaa ttccctatgc cgtagaggtg gtagtggaaa gttataaaga
     2521 agagaaagaa aaaatataca tcgcagccct gattcttgtt gagcgtgaat cacaaaaagg
     2581 gattataatc gggcatcagg gcaaagccct gaaaaaattg gggactacgg ctcgccggga
     2641 gatagaacgt tttacgggga aaaatgtatt tcttgaattg tttgttaagg tcgaaaaaga
     2701 ctggcgtaca aacgagaaca gtttgaaaaa cttcggatac atccaggatt aatgacagaa
     2761 acatgggaaa tatagtagcc atagtaggcc gtcccaacgt ggggaaatcg actttgttta
     2821 atcgtttggt
//
LOCUS       DAOSTD010000140         2828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_32134_length_2828_cov_17.629643, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000140 DAOSTD010000000
VERSION     DAOSTD010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32134_length_2828_cov_17.629643"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLI37_07480"
     CDS             <1..279
                     /locus_tag="PLI37_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOQ58887.1"
                     /translation="VFNTQGLSWLGIGLVITVLPPLIVGILARSVFKLNYLDIMGLLS
                     GSMTDPPALAYANSSSGSDQPVVAYSTVYPLTMFLRVLCAQILILIFA"
     gene            280..2757
                     /locus_tag="PLI37_07485"
     CDS             280..2757
                     /locus_tag="PLI37_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOQ58888.1"
                     /translation="MIYPQNFEIKTGFDQIRLLLQEQCSSTLGKEKVLEMRFSDNYKD
                     ILLALELSAEFLAVLHGEQTFPEILFFDLRAVLKRLQVEGSYTDQKELFDLKRSLDSY
                     HKVLHFLNHKEQATLLYPKLKELAAGQQTFPRIIEQIDSILDKSGEIQDHASPELAQI
                     RKKISITTAGISRKLHILIKKAQEEGIVEKGSSPSVRDGRLVIPVPPAHKRKISGIVH
                     DESASGKTVYIEPAELVEANNQVRELQSEEKREILRILIQFADYIRPLNEELLAAFDY
                     MAQIDFIRAKALLAKKTNALKPQLSDQSVVNWKGAIHPLLFLSLQKQGKEIVPLDIQL
                     DEQQRILVISGPNAGGKSVCLKTTGLLQYMLQCGLLIPVQENSVCGIFSKLFIDIGDE
                     QSIENDLSTYSSHLNNIKFFLKNSDKKTLLLIDEFGSGTEPKIGGAIAEACLDLFKQK
                     GLFGVITTHYDNLKHYAEDNEGVVNAAMLYDRHLMQPLFRLEIGRPGSSFAVEIARTI
                     GLPREVIDKATEIVGSDHIDMDKYLQDIVRDKRYWEKKRLEIKRLEKDLAQLTTDYRE
                     QMELIKQERKQILGKAKTQAEQLLSRANAEIEGTIKSIRESQAEKEQTKLLRANLDKF
                     KQQALKEEHEAGHSLKPKASQTTARKQKILSKLESKTQKKAAVETEINFVPGDIVHLK
                     GQNVPGKVLEVKGGKITVAFGSIKSTVTPDKLEKSKAAKIKDLPDKSLGLKISEDVHR
                     IGLNFKQEIDLRGLRGDEALQQVMYYIDEAVVVGVSSVRLLHGTGTGALRTLIRNYLG
                     TVPEVKSFRDEHVQLGGAGITVVEFKH"
BASE COUNT          883 a          691 c          627 g          627 t
ORIGIN      
        1 gttttcaata cccagggatt gagttggctt ggcataggtt tggtgattac cgttttacca
       61 ccgcttatcg ttggcatttt agcccgcagt gtttttaaac tgaattatct ggatattatg
      121 ggactactgt cgggaagtat gaccgatcct cccgccctgg cttacgctaa cagcagttcc
      181 ggcagcgatc aacctgttgt agcttactct actgtttatc ctttgaccat gtttttacgc
      241 gtcctttgcg ctcagatact gattttgatc tttgcctaaa tgatctatcc gcagaatttt
      301 gaaataaaaa caggctttga ccaaatccgc cttttgctac aagaacaatg cagcagtact
      361 ttgggtaagg aaaaagtctt ggaaatgcgt ttttccgaca attacaaaga catactcctt
      421 gccttggaac tcagcgcgga atttttggct gtattacatg gtgaacagac ttttcccgag
      481 attttatttt tcgatctaag agctgtgctg aaacgccttc aggttgaagg cagttatacc
      541 gaccaaaaag aactgttcga tttaaaacgc tcattggaca gttatcataa ggtattgcat
      601 tttttaaacc acaaagaaca agctacactt ctctatccca agctaaaaga gcttgctgcc
      661 ggacaacaaa ctttcccacg aataatagaa cagatagaca gcatcctcga taaatccggc
      721 gaaatacagg atcatgcctc cccggaactg gcacaaatcc gaaaaaaaat ctccattact
      781 acagcgggca tctcgcgcaa actgcacata ttgatcaaaa aagctcagga agaaggcata
      841 gtcgaaaaag gcagcagccc cagtgtacgc gacggccgcc tggtgattcc cgttcctccg
      901 gcccataaac gcaagatcag cggtattgta cacgatgagt cggccagcgg taaaactgtt
      961 tatatagaac cggccgaatt ggtagaagcc aataaccagg tcagggaact tcagagcgaa
     1021 gaaaaaagag agattctccg cattctcata caatttgccg attacatcag gcctctaaac
     1081 gaagaattgc tggctgcttt cgattatatg gcccaaatag actttatcag ggcaaaagca
     1141 ctcctggcca aaaaaacaaa cgcccttaag cctcaactgt cggaccaaag tgtcgtaaac
     1201 tggaagggag ccatacatcc cctactcttt ctttccctgc aaaaacaagg caaagaaatc
     1261 gtgcccctgg atattcagtt ggacgaacag cagagaatac tggtgatttc ggggcccaac
     1321 gccggaggaa agtcggtttg cctcaaaaca acgggtcttc tgcaatatat gcttcaatgc
     1381 ggcttattga tcccggtgca ggaaaattca gtttgcggaa ttttttccaa actttttatc
     1441 gacataggcg acgaacaatc catagaaaac gacttgagca cctatagctc acatctgaac
     1501 aacatcaagt tttttcttaa aaattcagac aaaaagacct tattgctgat agacgaattc
     1561 ggcagcggga ccgaacccaa aattggcggc gccatagccg aagcctgtct tgatctcttc
     1621 aagcaaaagg ggctgtttgg cgttatcacc acgcattacg acaatctcaa gcactatgcc
     1681 gaagacaacg aaggcgttgt aaacgctgcc atgctctacg accggcatct gatgcaaccc
     1741 ctgttccgat tggaaatagg ccggccgggc agttcttttg ccgtcgaaat tgcccgtacc
     1801 ataggcctgc cccgggaggt gatcgacaaa gccaccgaaa tagtaggctc agaccatatc
     1861 gacatggaca aatacttgca ggatatagta cgcgacaaac gttactggga aaagaagcgc
     1921 ctcgaaatca aacgtctcga aaaagacctt gctcaattga ccaccgatta ccgggagcag
     1981 atggaactca tcaaacagga gcgcaaacaa atactcggta aagccaaaac tcaagccgaa
     2041 caactgctta gccgggccaa cgccgaaatt gaaggcacca tcaaaagcat ccgggaatcc
     2101 caagccgaaa aagaacaaac caaactactg agggccaatc tcgataaatt caaacaacag
     2161 gctttaaaag aagaacacga agcgggacat tccctcaaac ccaaagcctc gcaaaccacc
     2221 gcccgcaaac aaaaaatact gagcaaactg gaatccaaaa cacagaagaa agccgctgta
     2281 gaaacggaaa tcaattttgt tcccggcgat atagtccatc tgaaaggcca aaacgttccg
     2341 gggaaagtgc tcgaagtaaa aggcgggaaa atcactgtcg catttggcag tatcaaatct
     2401 acggtcaccc cggacaaatt ggaaaaaagc aaggctgcca aaataaaaga ccttccggat
     2461 aaaagcctgg gactgaaaat cagtgaggac gttcatcgca tcggtttaaa ctttaaacag
     2521 gaaatcgacc tcagaggctt gcggggagac gaagctctgc aacaggtaat gtattatatt
     2581 gacgaagccg tcgtggtggg cgtttccagc gtccgccttt tgcacggcac cggtaccggg
     2641 gccctgagaa ccttgatacg caactacctg ggcaccgtcc ccgaagtcaa aagtttcaga
     2701 gatgaacacg ttcagttggg aggtgccggc ataacagtag tcgaatttaa gcattaggcc
     2761 gtgaaaaccc accaaaaaac cgactggctg cccgttacca aaaaagaaat ggaactcagg
     2821 ggctggga
//
LOCUS       DAOSTD010000141         2811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_32414_length_2811_cov_19.892235, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000141 DAOSTD010000000
VERSION     DAOSTD010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32414_length_2811_cov_19.892235"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1580)
                     /locus_tag="PLI37_07490"
     CDS             complement(<1..1580)
                     /locus_tag="PLI37_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOQ58889.1"
                     /translation="MNKTKKNKLRFIILSAALVCCLGVFYAFKAQDRNFLISKNFDIF
                     HSLFRDLNLFYVDSVDVDQAFSNAIDGLLSSYDPYTSYIPESQMSELKYMTTGEYGGI
                     GAVISKRGEAIIVAEPYENMPAQEAGLLFGDELVEIDGISMEGKTVSFASDCLKGQPN
                     TSVELLIKREGEKKLIKKTLQRKLIQMNPVEYAGLLRPGVGYVYLSSFTDKSYQELRQ
                     TLINLNKQGAQSLILDLRDNGGGLLSEAVSICNLFVDKGEVIVTTKGKAPQWDKTYRT
                     LMEPLFEDMPVVVLTNTLSASSSEIVAGALQDLDRAVILGSRTFGKGLVQTTRELAYN
                     GVLKVTTAKYYIPSGRCIQAVNYANGRSGRLPDSLTTQFSTRNGRPVRDGGGISPDVS
                     CVQKEYSDFCWQLVNNLYFFDFANDYYRKHPKITSVENFKLSEQDFNDFKNHIRNSDF
                     RYELHSKSKLNELKKTLESEGYWEDVSDDFKSLESKLVHDLDKDLETFRPDIERILSL
                     EIIRRYYYQKGEIQESLKT"
     gene            complement(1662..2639)
                     /locus_tag="PLI37_07495"
     CDS             complement(1662..2639)
                     /locus_tag="PLI37_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOQ58890.1"
                     /translation="MKNLLALGNALVDVLIRLDSEEILTELGIAKGSMQLIDKERFDL
                     IQQSLKPYKKQYASGGSAANTIIGTATLGLPSAFIGKIADDSNGAYFEKDMKRAGVET
                     RLLKGQSSSGTAFTFVSPDGERSFATYLGAAAELQAEDLTPQMFKGYANIYIEGYLLQ
                     NYDLILRIMELAKAAEMNIIWDLASYNIVEEHKTFLTEKVLPQVDYLFSNEMEAEALT
                     GAKADEALDLLAAGTKVAVVKMGAKGAMAKRGQEIARVPALKVNALDTTGAGDLFAAG
                     FLYGLFIGLPLNEAASIGNITAGNVVEVLGAKMDQTRWAAIKKQIKSLL"
     gene            2724..2797
                     /locus_tag="PLI37_07500"
     tRNA            2724..2797
                     /locus_tag="PLI37_07500"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2758..2760,aa:Asn,seq:gtt)
BASE COUNT          624 a          650 c          701 g          836 t
ORIGIN      
        1 gttttgagcg attcctgaat ttctcctttt tgataatagt agcgcctgat gatttcgagg
       61 ctcaagatgc gctcaatgtc gggtctgaag gtttcgaggt ctttgtccag atcgtggacc
      121 agcttggact cgagcgattt gaagtcgtcg ctgacgtctt cccaataacc ttcggattcc
      181 aacgtttttt tgagttcgtt caatttgctc ttactatgta attcatacct gaaatcgctg
      241 ttgcgtatgt ggtttttgaa gtcgttgaaa tcctgttcgc tgagcttgaa gttttcgacc
      301 gaagttattt tggggtgttt gcggtagtag tcgttggcaa aatcgaaaaa gtacagattg
      361 tttaccagtt gccaacaaaa gtcggagtat tctttttgga cgcaactcac atcggggctg
      421 atgcctcctc cgtctctgac cggccggccg ttgcgagtgg aaaattgcgt ggtcaggctg
      481 tcgggcagcc gtccgctgcg gccattggcg taattgacgg cctggatgca acgtccgctg
      541 gggatgtaat acttggcggt ggtcactttg agcactccgt tataggccaa ttctctggtg
      601 gtttgtacca ggcccttgcc aaacgtacgg ctgcccaaaa taacggccct gtctaaatct
      661 tgcaaagctc cggccacaat ttccgaagaa gaagccgaaa gcgtatttgt aagcacgacc
      721 accggcatat cctcgaaaag cggctccata agcgttctgt aggtcttgtc ccattgaggc
      781 gctttgcctt tggtggtgac gatgacttca cctttgtcta caaacaagtt gcagatggaa
      841 accgcttcgc ttagtaagcc ccctccgttg tcgcgtaaat cgaggatgag actttgagcg
      901 ccttgtttgt tgagattgat caaggtttgt ctgagttcct gataggattt gtcggtgaag
      961 ctgctcagat atacgtagcc cacgcccggt ctcagcaagc cggcatattc tactggattc
     1021 atctgtatga gtttacgttg cagcgttttt tttataagct tcttttcgcc ttcgcgtttt
     1081 atcagcaatt ctacgctcgt gttgggctgg cctttcaggc agtcgctggc aaaggatacg
     1141 gttttgcctt ccatgctgat gccgtcgatc tcgaccaact catctccaaa aagcagtccg
     1201 gcttcctgag cgggcatgtt ttcgtaaggt tccgcaacga tgatggcttc accccttttg
     1261 ctgatgacgg ctcctatgcc tccgtattcg ccggtggtca tgtatttcag ttccgacatt
     1321 tgcgattcgg ggatgtagct ggtgtaggga tcgtaggaac tcaacagccc gtctatggcg
     1381 ttggaaaacg cttgatccac atcgacactg tctacgtaga acaaattgag atctctgaag
     1441 agtgagtgga aaatgtcgaa atttttactg atcaaaaaat ttctatcctg agccttaaaa
     1501 gcataaaaaa ctcccaggca gcacaccagc gcagcagaca ggattataaa gcggagttta
     1561 tttttcttgg ttttgttcat cggttcttct tggatatatg ctttttttga tccggatgca
     1621 aggccgccgg aaacttgttt tcgccgcgat ctgatccgaa attaaagcaa cgatttgatt
     1681 tgttttttga tggcggccca tctggtttgg tccattttgg ctcccagaac ctctaccacg
     1741 ttgccggcgg tgatgttgcc gatgctggcg gcttcgttca ggggcaggcc gatgaacagg
     1801 ccgtataaaa agccggctgc gaataaatcg ccggctccgg tagtgtcgag agcgtttact
     1861 ttgagggccg ggactcgggc tatttcctga cctcttttgg ccatggctcc tttggctccc
     1921 attttgacga cggcaacttt ggttccggcc gccaacaaat cgagggcttc gtcggctttg
     1981 gcgccggtaa gcgcttcagc ttccatttcg ttcgaaaaca ggtaatctac ctgaggaagt
     2041 accttttcag ttaaaaaagt cttgtgttct tctacaatat tgtaactggc cagatcccag
     2101 atgatgttca tttcggctgc tttggccagt tccatgatac ggagtatcaa atcgtaattt
     2161 tgcagtaaat aaccctctat atatatattg gcataacctt tgaacatctg tggtgttaaa
     2221 tcttcggctt gcaattcggc ggcagctccc aggtaggtgg caaaactgcg ctctccgtcg
     2281 gggctgacaa aagtaaaagc cgttcccgag gaggattgtc ctttgagcag acgggtttcc
     2341 acacctgccc gcttcatgtc tttttcgaaa taggctccgt tgctgtcgtc ggcaatcttg
     2401 cctataaaag ctgaaggcaa gcccaaagtg gcggtgccga tgatggtatt ggcggcggat
     2461 ccccccgaag catattgttt tttataaggc ttgagcgatt gttgaatcag atcgaaacgt
     2521 tctttgtcga tcaattgcat gctaccttta gctatgccga gttctgtaag gatttcttcc
     2581 gaatcgaggc ggattaacac atccaccaga gcgttgccga gggctaataa attcttcatt
     2641 ctgtaaattt tttggcaaag atattgcaat attcgacaat atgccgtatt tttgtcgcgc
     2701 ttttttaaat gtaagctaaa cattcttcag tagctcagtc ggttagagca tctgactgtt
     2761 aatcagaggg tcgtaggttc aagtcctacc tgaagagcta aagagtccga a
//
LOCUS       DAOSTD010000142         2713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_34009_length_2713_cov_15.155380, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000142 DAOSTD010000000
VERSION     DAOSTD010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34009_length_2713_cov_15.155380"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..>1086)
                     /locus_tag="PLI37_07505"
     CDS             complement(47..>1086)
                     /locus_tag="PLI37_07505"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malectin"
                     /protein_id="HOQ58891.1"
                     /translation="SLTKGKSSHNYIYRVNCGGDDYIDSFGQLWAKDDTSFSRSWAQD
                     FVGLNPYQASQRVTYDPIRGSRDWPLFGHFRYGRHKLAYRFPLPDGKYRLELYFIEPW
                     HGTGSGKGGDCAGLRLFDVSANGKILLNDLDIWAESGHDVALKKTFRVKVQGGFLNLE
                     FPEIKAGQAVLSAIAIASRDKSIKPYVPPVSDWSWQKADRERVGRFPTERLPEDNNVR
                     PGVSYPAVEAVVEGNYRKEQMNNRMAVIFEAASTNSISWDITTGLAQLYALRFNYRNI
                     SGRDMLVDMKIIAADGTVLKEDKLTFPINERGWKLISTTTGSFINAGHYRIVLSSPDM
                     DGLCFERLDVQ"
     assembly_gap    1087..1186
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1187..>2713)
                     /locus_tag="PLI37_07510"
     CDS             complement(<1187..>2713)
                     /locus_tag="PLI37_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOQ58892.1"
                     /translation="DLGRHLGGRVTFTIDVSDHLNYGADNVLEVKVEHPHMISDMPCV
                     CGGCSSEWGFSEGSQPFGIYRPVVMEVTGEVRIEPFGVHIWNDDRATKIFIDTEIRNY
                     SGREQKLLLENRFNTHKGKKVFLLREEISVAPGATVTVRQEADIENPVLWSNENPYLY
                     QLVTSLKQGRKKKDALETPFGIRTISWPVKRNDGDGRFFLNGKPVFLNGTCEYEHLLG
                     QGHAFSREQVEARVKQMLNAGFNAFRDAHQPHHLDYQKYWDKYGILFWTQLSAHIWYD
                     TPVFRENFKTQLRQWVKERRNSPSVVLWGLQNESMLPKDFAEECTAIIREMDPTALNM
                     RAVTTCNGGVGTDWNVVQNWSGTYSGNPERYDEELALPNQLLNGEYGAWRTLDLHEAP
                     GPFKQEGAYSEERFCQLMEMKIHLAEKARDKVCGQFQWIFNSHDNPGRRQPDEAYRLI
                     DRVGPVNYKGLITPWEEPLDAYYMYRSNYVPADTDPMVYIASHTWPDRFEEPCRASIQ
                     V"
BASE COUNT          613 a          701 c          619 g          680 t
ORIGIN      
        1 ttaagttttt tagtggctca gacacacttt ttgaaacagt atcttttcac tgcacatcaa
       61 gcctttcgaa acacaggcca tccatatcgg gcgaagaaag aacaatccgg taatgaccgg
      121 cgttgatgaa cgaaccggtg gtggtgctga tcagcttcca gccccgttca ttgatgggga
      181 aagtcagttt atcttctttg agcacggtgc cgtctgcggc tattatcttc atatccacca
      241 gcatatcgcg gccggagata ttccggtaat taaaacgcaa agcgtagagc tgagccagtc
      301 cggttgtaat atcccaactt atgctgtttg tgcttgctgc ttcaaaaatc acagccattc
      361 tgttgttcat ctgttctttc ctataattgc cttcgacaac ggcctccacg gcgggataag
      421 acacaccggg cctgacattg ttgtcttcgg gcaatcgctc ggttggaaaa cgccccaccc
      481 gctcccggtc tgccttttgc cagctccagt ccgaaaccgg aggcacataa ggcttaatgc
      541 ttttgtcgcg gctggcaatg gcgatagccg acaaaacggc ctgaccggct tttatctcag
      601 gaaattcgag gtttagaaaa ccgccttgta ctttcacccg gaaagtcttt ttcaaagcga
      661 catcgtgacc cgattcggcc catatatcca ggtcgttcaa gagaattttt ccgtttgcgg
      721 aaacatcaaa aaggcgcaga ccggcgcaat cgccgccctt acccgatcct gtaccgtgcc
      781 agggttcgat aaaatacaac tccagcctgt acttaccgtc cggcagagga aaacggtaag
      841 ccagtttatg cctgccgtaa cggaaatgac cgaacagagg ccaatcccgg cttccgcgaa
      901 tgggatcgta ggtgacacgc tgactggcct gatagggatt gagaccgaca aaatcctgag
      961 cccaggaacg ggagaaactg gtatcgtcct tggcccaaag ctggccaaaa ctgtctatgt
     1021 agtcgtcgcc tccgcaattg acgcgataaa tgtaattatg cgaagacttg cctttggtca
     1081 gggattnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnactt gtatgcttgc
     1201 cctgcaaggc tcctcaaaac gatcgggcca ggtatgcgaa gctatataga ccatgggatc
     1261 cgtatcggcc ggcacataat tggaacgata catgtaataa gcatccaaag gttcttccca
     1321 gggagtaatg agccccttgt aattgaccgg ccccacccga tctattaatc tgtaagcttc
     1381 gtcgggctga cggcgtccgg gattgtcgtg gctgttgaat atccattgaa actggccaca
     1441 aactttgtcg cgggcttttt cggccaaatg gattttcatc tccatcaatt gacaaaaacg
     1501 ttcttccgaa taggcgcctt cctgcttaaa gggtcccggt gcttcgtgca gatccagggt
     1561 tctccaggct ccatattcac cgttgagcaa ctgattgggc aaagccagtt cttcgtcgta
     1621 acgttccgga ttgccgctgt aagtgccgct ccagttttgc accacgttcc agtcggtgcc
     1681 taccccaccg ttgcaggtgg taaccgcacg catatttagc gccgtgggat ccatctcccg
     1741 aatgatggcg gtgcattctt cggcaaagtc tttgggcaac atgctttcgt tttgcagtcc
     1801 ccaaagcacc accgaagggc tgtttcggcg ctcttttacc cattgcctga gctgagtttt
     1861 gaaattttct ctgaatacgg gggtgtcgta ccagatatgc gccgatagct gtgtccaaaa
     1921 taaaattccg tatttatccc aatatttctg ataatcgagg tgatggggct gatgggcgtc
     1981 tctgaaagca ttgaaaccgg cgttgagcat ttgctttaca cgggcctcca cctgttcacg
     2041 gctaaaagca tggccttgcc ccaacagatg ttcatactcg caagtaccgt tcaaaaacac
     2101 cggcttgccg ttcaaaaaga aacgcccgtc gccatcgttg cgcttcaccg gccagctgat
     2161 ggtgcgaata ccgaaggggg tttccaatgc atcttttttc tttcttcctt gcttaagact
     2221 ggtaaccaat tgatacaaat aaggattttc gttgctccat aataccggat tttctatgtc
     2281 tgcctcctgg cgtacagtaa ctgtcgctcc cggagccaca ctgatctcct cgcggagcag
     2341 aaaaactttt ttgcctttgt gcgtattgaa acggttctcg agcagaagct tttgttcccg
     2401 gccggagtaa ttcctgattt cggtatcgat aaagattttc gttgccctgt cgtcgttcca
     2461 gatgtggacg ccgaaaggtt ctatgcggac ctcgccggta acttccatta ccaccggccg
     2521 gtagatgccg aagggctgcg agccttcgct aaaaccccat tcggaagaac agcctccgca
     2581 cacacagggc atatccgata tcatatgcgg atgctctacc tttacttcca atacattgtc
     2641 tgctccgtaa ttcagatgat cgctcacatc gatcgtaaac gttaccctgc ctcccaaatg
     2701 acgacctaag tcc
//
LOCUS       DAOSTD010000143         2620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_35651_length_2620_cov_11.242495, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000143 DAOSTD010000000
VERSION     DAOSTD010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35651_length_2620_cov_11.242495"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..501
                     /locus_tag="PLI37_07515"
     CDS             250..501
                     /locus_tag="PLI37_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HOQ58893.1"
                     /translation="MENLGLGLLLMVVGMPTVFLILLIIIYLGKGLIVFVNKFVPEEL
                     IVKNKHPESGIPDRVKRIIAAAVSEATKGKGIVTTIEKR"
     gene            505..2343
                     /locus_tag="PLI37_07520"
     CDS             505..2343
                     /locus_tag="PLI37_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HOQ58894.1"
                     /translation="MKRREVKFSLMFRDMWQSSGKYVPRVDQLVKIAPHIVKMGCFAR
                     VETNGGGFEQVNLLFGENPNKAVREWTKPFHEAGIQTHMLDRGLNGLRMSPVPADVRK
                     LFYKVKKAQGTDITRTFCGLNDLRNIAPSITYAKDAGMISQCSLCITHSPIHTVEYYT
                     NMALELIKMGADEICIKDMAGIGRPVSLGKIVENIKKAHPEIPVSYHSHAGPGFDVAS
                     ILEVCRGGCDYIDVGMEPLSWGTGHPDVITIQEMLKDAGYDVPEINMDEYMKVRTMIQ
                     EFMDDFLGLFIPERNRLMNSLLIKPGLPGGMMGSLMNDLNSNLININKYKVKHNLPEM
                     DQDELLIKLFDEVAYVWPRVGYPPLVTPFSQYVKNLALMNVMQLEKGRERWAFIADDI
                     WDMILGKAGRLPGKLAPEIIEKARQEGREFFDGDPQDNYPDVLDDYRKLMKEKNWELG
                     QDDEELFEYAMHPAQYEAYKSGKAKTDFLDEVEKMRQAGDCRELPKEPKTITVELEGE
                     QYLVTVTFDDKAPAAIVTEDNGEPVAGGGKELLSPLEGNFYLTKNTQETPLKVGDYVK
                     KGDVVCYIEAMKTYNAISSEYDGKITEILHKPGDAVGEDDVLMKIK"
     gene            2350..>2620
                     /locus_tag="PLI37_07525"
     CDS             2350..>2620
                     /locus_tag="PLI37_07525"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOQ58895.1"
                     /translation="MDNIFDKLYDMTAFSNIFAEPQFLIMYAIAFILLYLGIKKQYEP
                     LLLVPIAFGVLLANFPGGGMGVVQADQNGMVEVQGVMKSIWEMPLH"
BASE COUNT          747 a          589 c          626 g          658 t
ORIGIN      
        1 attacgattt cctgtacaaa gcaggggttg ccggcatttt tggcccgggc acttccgtcg
       61 ctaaggccgc ttgtgaaatc atgaagattt tgttggacga ggaataagcc ctgagctttt
      121 tgaacttcgc cccaaaatct ttaatcgccc ggctctttac agccttgttg tcttgttttt
      181 tttaagtacc tttgtggcgt ttttttctaa tttgagctta aggtcgtata tagcaaagag
      241 ataagcatta tggaaaatct tggtcttggt ttgttgttga tggtagtggg aatgcccacc
      301 gtctttttaa tccttcttat catcatttac ttgggcaaag gtcttattgt ttttgtcaac
      361 aagttcgttc ccgaagaact tatagtaaag aacaagcatc ccgaaagtgg aattcccgac
      421 agagttaaaa gaattattgc cgccgccgta tcggaggcca ctaaaggcaa aggtatagtt
      481 acaaccatag aaaagaggta atttatgaag agaagagagg ttaaattcag cttgatgttc
      541 agggacatgt ggcagtcgtc cggaaaatac gtccccagag tggatcaatt ggtaaaaatt
      601 gcaccccaca tcgttaaaat gggttgcttt gcgcgtgttg aaaccaacgg aggaggattt
      661 gaacaagtca acctgttgtt tggggaaaat cccaataagg cagttcgcga atggaccaaa
      721 ccctttcacg aagcaggtat acagacccac atgctcgaca ggggattgaa cggcctacgc
      781 atgagtccgg tacccgccga tgtacgcaaa ttgttttata aagtcaaaaa agcccagggt
      841 accgatatta ccagaacttt ctgcggattg aacgatctgc gcaatatagc tccttccatt
      901 acctatgcca aagatgccgg catgatttca caatgttcgc tctgcataac acattcccct
      961 attcataccg tagaatatta caccaacatg gctttggagc tcatcaaaat gggtgcagac
     1021 gaaatttgca tcaaggatat ggccggtata ggacgtccgg tatccttggg taaaattgtc
     1081 gaaaacatca aaaaagccca tcccgagatt cccgtgtctt accacagcca tgccggcccg
     1141 ggttttgatg tggcttccat tctcgaagtt tgccgcgggg gttgcgacta cattgatgtc
     1201 ggcatggagc ctctttcctg gggcaccgga catcccgatg ttatcaccat tcaggagatg
     1261 ctgaaggacg ccggttacga cgttcccgaa atcaacatgg acgaatacat gaaagtccgc
     1321 actatgattc aggaatttat ggacgatttt ttgggcctgt ttattccgga aagaaaccgt
     1381 cttatgaatt cactgcttat caaacccggt cttcccggcg gcatgatggg cagcctgatg
     1441 aacgatctga acagcaacct gataaatata aacaaataca aggtcaagca taatttgccc
     1501 gaaatggatc aggacgaact gcttatcaag cttttcgacg aagtggctta tgtttggccc
     1561 agagtgggtt atcccccatt ggtcactcct ttcagccaat acgtcaaaaa cctcgccctg
     1621 atgaacgtaa tgcagctcga aaaaggcaga gaacgctggg ctttcatagc cgacgacatc
     1681 tgggatatga ttttgggcaa ggcaggacgt ctgcccggca aactggcccc cgaaatcatc
     1741 gaaaaagcca ggcaggaagg cagggagttt ttcgatgggg atcctcagga caactatccc
     1801 gacgttttgg acgattaccg caaattgatg aaagaaaaga attgggaatt gggacaagac
     1861 gacgaagaat tgtttgagta cgccatgcac ccggctcaat acgaagccta caagagcggc
     1921 aaagcaaaaa cggattttct cgacgaagtc gaaaaaatgc gccaggccgg cgattgccgg
     1981 gaactgccca aagaacccaa aaccatcact gtggaactcg aaggagaaca gtacctggtc
     2041 accgttacat tcgacgacaa agctcctgca gcgatagtta ccgaagacaa cggagaaccg
     2101 gttgccggtg gtggaaaaga attgctgtcg cctctggaag gcaatttcta tctgaccaaa
     2161 aatactcagg aaactcctct caaagtaggt gattatgtta aaaagggcga tgtagtatgc
     2221 tatattgaag ccatgaagac ctacaacgcc atctcttctg aatacgacgg taaaataacg
     2281 gaaattctgc acaagccggg agatgctgtt ggcgaagacg atgtcttaat gaaaatcaag
     2341 taatgcccta tggacaatat cttcgataag ctttacgata tgactgcctt cagcaacatc
     2401 tttgctgaac cgcagttcct gattatgtac gccattgctt ttatactgct ttatttaggc
     2461 atcaaaaagc aatacgaacc actgttgctg gtacccattg cctttggtgt actgctcgcc
     2521 aacttccccg gtggcggcat gggagtcgtt caggcagacc aaaatggtat ggtagaagtt
     2581 cagggtgtaa tgaaaagcat ctgggaaatg cccctgcacg
//
LOCUS       DAOSTD010000144         2478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_38470_length_2478_cov_18.342138, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000144 DAOSTD010000000
VERSION     DAOSTD010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38470_length_2478_cov_18.342138"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLI37_07530"
     CDS             <1..609
                     /locus_tag="PLI37_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOQ58896.1"
                     /translation="DVETGSDIELTVDNQYILSPKDLKTIHFLNKMLDAGVEVFKIEG
                     RARGPEYVYTVVSCYREALTACLDRTFSEDKIKNWDLRLARVFNRGFWDGYYLGQRLG
                     EWSHRYGSSATTRKVRIGRGINYFSKLAVAEFQLESGELSLGDEILITGPTTGVIESR
                     VEEIRLEDRPVEKAVKGQRISIPLASKIRPSDTLYKVVPFRP"
     gene            complement(606..800)
                     /locus_tag="PLI37_07535"
     CDS             complement(606..800)
                     /locus_tag="PLI37_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOQ58897.1"
                     /translation="MERKLTRSRKDRMIGGVLGGLAEYLNIDPVLVRVLYCALSLFTA
                     GFPGILLYIIMLILIPEDRS"
     gene            873..1310
                     /locus_tag="PLI37_07540"
     CDS             873..1310
                     /locus_tag="PLI37_07540"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOQ58898.1"
                     /translation="MTEETALFEIRLKVRDYECDLQGIVNNANYQHYLEHARHEFLRS
                     IGLSFADFHYRGIDFVVTRVEIDYKTPLTSGDSFTVSLNIKKEGVRWLFFQEIRNEKD
                     GKLCVKGKVASVAVVNGRPKNCAEIDDVFNKILAERGSADSHS"
     gene            1424..>2478
                     /gene="dprA"
                     /locus_tag="PLI37_07545"
     CDS             1424..>2478
                     /gene="dprA"
                     /locus_tag="PLI37_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOQ58899.1"
                     /translation="MSQLHRIALSMLRCLSPQNARLLLEEVKTPESVFDLEVLKEIPG
                     LNPRLPEEVATGAALRQAERELDFMANSGVSLHWFEDEDYPARLRTCPDAPLLLYSMG
                     EAELNPSKILSIVGTRKMTAYGRMFCEQLVQDLAGAFPDLTIVSGLAYGVDICAHKAA
                     LDFNLKTLAVLAHGLDRIYPPAHRQTAKKIVHQGALLTEMPSFTQPLGWRFVQRNRIV
                     AGLSDACVVVESAEKGGSLITAYMAREYNREVFALPGRTIDPFSRGCNKLIKNQVAVL
                     LEKAEDLIREMNWDRNVKQQSRQQTLFPLLDAAMQEVFDLFAEGRDYDTSELYELLRQ
                     KRDCDMGYLLSILLQLE"
BASE COUNT          654 a          545 c          667 g          612 t
ORIGIN      
        1 gacgtggaaa cgggcagcga tatcgagctg acagtggaca atcagtatat tttgtcgccc
       61 aaggatttga agactattca tttcctcaat aaaatgctgg atgccggcgt ggaggttttc
      121 aaaatagaag gcagagcccg gggaccggaa tacgtgtaca cggtggtgag ctgttatcgc
      181 gaagccttga ccgcctgtct cgaccggact tttagcgaag ataaaatcaa gaactgggat
      241 ctgaggctgg ccagagtgtt caaccgggga ttctgggacg gttattattt ggggcagagg
      301 ctgggagagt ggtcacatcg ctacggctct tcggcaacca cccgcaaagt acgtataggc
      361 cgcggtatca attatttttc caaactggct gtagccgagt ttcaattgga aagcggcgaa
      421 ctgagcttag gagatgaaat tctgattacc ggccctacaa cgggcgttat cgaaagcagg
      481 gtagaggaaa taagactgga agaccggccg gtggaaaaag cagtaaaggg ccagcggatt
      541 tccatccctc tggcaagcaa gatcaggcca tcggatacct tatataaggt ggttccgttc
      601 aggccttagc tccggtcttc ggggatcaga atcagcataa tgatgtacag caagattccc
      661 gggaagccgg ctgtaaataa acttaaggca cagtagagta ccctgaccaa aacgggatca
      721 atattcaggt attctgcaag ccctccaagt acgccgccta tcatgcggtc tttgcgtgaa
      781 cgtgttaatt tcctttccat agggtaatgt aaattaagac aggtaaattt gtgcgcaaaa
      841 atatgtttta tatgtaaatc aatctaataa ttatgacaga agaaaccgct ctttttgaaa
      901 ttcgcctcaa agtaagggat tacgaatgtg atttgcaagg catcgtgaat aacgctaact
      961 atcagcacta tctggaacac gccaggcatg aatttctgcg cagtataggt ctgagtttcg
     1021 ccgattttca ctacaggggc atcgattttg tggtgaccag agtggaaata gattataaaa
     1081 cgcctttgac cagcggagac agctttacgg taagcctgaa cataaaaaaa gaaggcgttc
     1141 gctggctgtt tttccaggag atcagaaacg agaaagacgg taaactttgt gtcaagggca
     1201 aagtcgcttc ggttgctgtg gtaaacggac gccccaaaaa ttgcgcggag attgatgatg
     1261 tcttcaacaa aatattggca gaaagggggt cggccgacag tcattcctaa agatctatca
     1321 atttcagggc gaaaaatagt aaccggggtt cagtattctg aacgctgtgg tttttgcggc
     1381 ttacatagtc tcgggctaaa tttgcaacca cagaaaagca cttatgtctc agttacaccg
     1441 tatagcattg agtatgttgc gttgcctcag tccgcaaaat gcacgcctat tgctcgaaga
     1501 agtaaaaacg cccgagtccg ttttcgacct ggaagtccta aaagaaattc cgggacttaa
     1561 tccacgatta ccggaagaag tagcgacggg tgcggctctg aggcaggccg agcgcgaatt
     1621 ggattttatg gcaaacagcg gggtaagtct gcattggttt gaggacgaag attatccagc
     1681 ccgtttgcgc acttgccccg atgctccctt gttgctgtac agcatgggcg aagccgaatt
     1741 gaatccctcc aaaatacttt cgatagtggg tacccgcaag atgacagcct acgggcgtat
     1801 gttttgcgag cagcttgttc aggatctggc cggcgctttc cccgatctta ccattgtgag
     1861 cggtctcgca tacggggtgg atatatgtgc tcacaaagcg gccctggatt tcaacctgaa
     1921 aaccctggct gtgttggctc acggcctgga tcgtatttat cctcccgcac acaggcaaac
     1981 ggccaaaaag attgtgcatc agggtgctct gctcacggag atgccttcgt ttacccagcc
     2041 tctcggttgg cgttttgtac agcgcaaccg catagtggcg ggcttgtcgg atgcctgtgt
     2101 ggtagtggaa tcggccgaaa aagggggctc tttgatcacg gcctatatgg ccagagaata
     2161 caaccgcgaa gtttttgcct tgcccggccg gacaatcgat cctttttcgc ggggttgcaa
     2221 taagttgatc aaaaatcaag tggcggtttt gctggaaaaa gccgaagatc tgattcggga
     2281 gatgaactgg gaccggaacg tcaagcaaca aagccgtcag caaaccttgt tccccttgtt
     2341 ggatgccgct atgcaagagg tgttcgattt atttgccgag ggcagggatt acgatacttc
     2401 cgaattgtat gaactcttgc ggcagaagcg cgattgcgac atgggttatt tgctgtcgat
     2461 cttgttgcaa ctcgaatt
//
LOCUS       DAOSTD010000145         2450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_39047_length_2450_cov_14.780793, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000145 DAOSTD010000000
VERSION     DAOSTD010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39047_length_2450_cov_14.780793"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..>2450)
                     /locus_tag="PLI37_07550"
     CDS             complement(37..>2450)
                     /locus_tag="PLI37_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOQ58900.1"
                     /translation="FFSCKDNNPLDESYPEWLGSSIYGYLEKQGNYSVFLQLIDDLQY
                     DQVLKLTGSKTLFVADDEAFDVFFNAENVWGVSSYSDLSMAQKKLLLNSAMINNAYLI
                     DKLSSVPGPEEGKCLRRTTAVSVYDSVPLVQAQELPDTKYWQRFKDNDRSLLLMKDFT
                     PVPMVHFLRRQLENNNITDEDFSIIFNGYNRQINDAHINGIRVIDQNIVCQNGYVHKL
                     EKVMLPLDNMAEVLRTHPATTKYSRLMERFSAPYYSAAATANYQRIYGGTDSVFVKRF
                     FSQRSAEGGNLTDPDGYATTGVLKFDPGWNTYYSESEELAMEQDMAAMLVPSNAALDF
                     YWEHDGRALKDYYVVWDSVPDKVLVKLLNNNMLNSFNASVPSKFDKVTNDANDAMGLT
                     IADVDCCLIANNGVIYITNKVFGPTSYISVSFPALINESMSIINWAVEELEFNAYLNS
                     QDSYYSFIIPVNDALKYYIDPVSYGKSKTKLFEFYYDPTETNVVNRVKANKFTIDLDT
                     WTKSDSSSATTAEIQNRLDDIVDNLIVIGDISDGRTYYKTKAGGIIKVTDANLGEGGM
                     KIAGGYQVEQDTFVTVRTIYDQSVSGNGKSYIVEAPLLSSKKSLYATLKEKPEFKVFY
                     DLMEGSDFFVTQMNKHSCVDRNCNLFNAFNYTVYVPTNESLQALLDDGTLPDWEKYEL
                     ETDVEIKKAIKDSIESFLRYHIQDNSVYLDKAAINGNFETSAINRETGRFYKLTVEGN
                     GSQGLFITDLMGNRRRVLQSDPNLYNLMVREYLYDTADKEKATTIFASSYAAVHQIDA
                     PLFFR"
BASE COUNT          632 a          553 c          575 g          690 t
ORIGIN      
        1 gatcttacaa gcctttctaa gcactcgaat aaaaaactat ctgaaaaata aaggagcatc
       61 tatttgatgc acagccgcat aggaggaagc aaaaatagta gttgcttttt ctttgtcggc
      121 cgtatcgtac aggtattctc ttaccatgag gttgtaaaga ttgggatcgc tctgcagtac
      181 ccgccggcga ttgcccatca ggtcggtaat gaacaggcct tgcgatccgt tgccttcgac
      241 cgtcagttta tagaagcggc ccgtctcccg gtttatggcc gaagtttcaa agttgccgtt
      301 gatagcggcc ttatccaaat aaaccgagtt atcctgaatg tgatacctaa ggaaactttc
      361 gatagaatcc ttaatggctt ttttgatctc gacgtcggtt tccaattcat atttttccca
      421 atcgggcaaa gtgccgtcgt cgagcaaagc ctgcaaagac tcgttggtcg gtacgtaaac
      481 ggtatagtta aaggcgttga acaaattgca gttcctgtcg acacaagagt gtttgttcat
      541 ttgagttaca aagaaatcgc tgccttccat caaatcgtaa aacactttga attcgggttt
      601 ttccttcaaa gtggcataaa gagacttttt ggagctgagc aagggagctt ccacaatata
      661 agatttcccg ttacccgaaa ccgattgatc gtaaatggtt cggacggtaa caaaggtatc
      721 ttgttccacc tggtagcctc cggcaatttt catgccccct tcgcccagat tagcatcggt
      781 cactttgatg attccacccg ccttggtttt gtagtaagtg cgtccgtcgc tgatatcccc
      841 tatcacgatg agattgtcga ctatatcgtc gagacggttt tgaatttcgg cagtagtggc
      901 cgaagaggag tcggatttgg tccaggtatc cagatctatg gtaaatttat ttgcttttac
      961 tctgttaacg acatttgttt cggtgggatc gtaataaaac tcaaacaatt tggtcttgct
     1021 tttaccgtag gaaaccgggt cgatgtaata tttcaaagca tcgtttaccg gtataataaa
     1081 gctgtaatag ctgtcctggg aatttaggta agcattgaac tccaattcct ccaccgccca
     1141 gttgatgatg ctcatgcttt cgttgatcaa tgccggaaac gaaaccgaaa tatagctggt
     1201 agggccaaag actttgttgg tgatgtaaat cactccattg ttggctatca ggcaacaatc
     1261 cacatcggca atggtcagtc ccatggcatc gttggcatcg ttggtgactt tgtcgaattt
     1321 gctgggaact gaggcattga aagaattgag catattattg ttgagcagtt ttaccagcac
     1381 tttgtccggc acactgtccc aaaccacgta ataatcctta agggcacgac cgtcgtgttc
     1441 ccagtaaaaa tcaagggctg cattcgaagg caccagcatg gcggccatat cctgttccat
     1501 ggccaattct tccgattcgg aataataggt gttccaaccc ggatcgaatt tcaacacccc
     1561 tgtggtagca taaccgtcgg gatcggtgag attaccgccc tcggcgctgc gttgggagaa
     1621 aaagcgtttt acaaaaaccg aatcggtgcc gccatagata cgctgatagt tggcggttgc
     1681 ggcagcgctg taataggggg cggaaaaacg ctccatcagt ctgctgtact tagtcgtagc
     1741 cgggtgggtt ctgagcactt cggccatgtt gtcgagaggc aacatgacct tttccagttt
     1801 atgcacatag ccgttctgac aaacaatgtt ctgatcaatc actctgattc cgttgatatg
     1861 tgcatcgttg atttgccggt tgtatccatt aaaaataata ctgaaatctt cgtccgtaat
     1921 attattgttt tcgagctgac gacgaagaaa atgcaccata ggcacaggcg taaaatcttt
     1981 catcagcaac agggaacgat cgttgtcttt gaaacgctgc cagtatttgg tgtcgggcaa
     2041 ttcctgtgct tgcaccaggg gtactgaatc gtacaccgat acggctgtag tgcggcgcag
     2101 gcatttaccc tcctcaggcc cgggtacgct ggagagtttg tcgatcagat aggcgttgtt
     2161 gatcatggcc gaattcagca ggagcttttt ctgtgccata cttaaatcgc tgtatgagct
     2221 tacaccccat acattttcgg cattgaaaaa aacgtcaaaa gcctcgtcgt cggcaacaaa
     2281 caaggtcttg ctgccggtca gctttaatac ctgatcgtat tgaaggtcgt cgatgagttg
     2341 caaaaaaaca ctgtaattac cctgcttttc gagataaccg tagatgctgc tccccaacca
     2401 ttcgggatag ctttcgtcca agggattgtt gtctttacag gaaaaaaaca
//
LOCUS       DAOSTD010000146         2420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_39728_length_2420_cov_16.056660, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000146 DAOSTD010000000
VERSION     DAOSTD010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39728_length_2420_cov_16.056660"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PLI37_07555"
     CDS             complement(<1..593)
                     /locus_tag="PLI37_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ58901.1"
                     /translation="MLSRLHIENYVLIEHLDLQFESGFSVITGETGAGKSVLMGAISL
                     ILGQRADLRLIKEGASRCVVEAEFNQMQHLSDFFARQGMEFDPESCIIRRELYENGKS
                     RAFVNDSPVALNTLRDLAIKLVDVHSQHESLLLDNSLFQLELLDNLAQSREELTAYRL
                     VYENYRQASQELKTLEESLQKDIDEQDYLLFQVEQLRS"
     gene            complement(598..1515)
                     /locus_tag="PLI37_07560"
     CDS             complement(598..1515)
                     /locus_tag="PLI37_07560"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOQ58902.1"
                     /translation="MNSFKNIHISFVFLFLFFLSGRGLALAQELNCRVQVNSDQIGGT
                     DKAVYESLRNAVTAFMNERQWSNAPIAQNEKIDCNLLFIIQSSENNVHKAELQIQSRR
                     PVFGSSYTTSLLNIRQNLEFEYLENQQLEFNETNLQSNLTATLAFWTYIVLCMDFDSF
                     APYGGERFLTKASDIVSQAQGFLGDNWKANEDDKNCWGWTNALNDENQKPMRLLNYNY
                     HRQGMDLLHQDAAAARKKITDSFKVLEEVKRLNPSSPLLSNLADSKIEEWIQLYSKAE
                     SKEKQEVFDLLSFIYPGQSNRLQAIKNQN"
     gene            complement(1512..>2420)
                     /gene="coaBC"
                     /locus_tag="PLI37_07565"
     CDS             complement(1512..>2420)
                     /gene="coaBC"
                     /locus_tag="PLI37_07565"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOQ58903.1"
                     /translation="MANGIADNMLITTYLSMKAPVFVAPAMDLDMYRHPSTTHNLELL
                     KRYGNHIIEPASGELASGLEGKGRMEEPEQIVAQLERFFSAQLDLKDKKVLITAGPTY
                     EKIDPVRFIGNYSSGKMAYALAEACANRGAQVELISGPVSLQAHHPAIHRTDVETAAE
                     MYEACMHYFPAVDVAILCAAVADFSPENPRPSKIKSNKEGFGLSLKPTKDIALNLGNV
                     KKPGQVLVGFALETDNEIENARAKMERKKLDFIVLNSLNDQGAGFKADTNKIKILEPG
                     GKVLDFPLKSKQKVAEDIVNHLITKL"
BASE COUNT          602 a          560 c          558 g          700 t
ORIGIN      
        1 gagcgcaatt gttccacttg aaaaaggaga taatcctgtt catcaatatc cttctgcagg
       61 ctttcttcca gcgttttcaa ttcctgagaa gcctgacggt aattttcgta caccaagcga
      121 taggctgtca attcctcccg gctttgagcc aggttgtcca acaactccaa ttgaaacaaa
      181 ctgttgtcga gcaaaaggct ttcatgttga gagtgtacgt caaccaattt tatggccagg
      241 tcacgcagcg tgttcaaagc caccggcgaa tcgttgacaa aagcacggct tttgccgttt
      301 tcgtacaatt ccctgcggat aatacaggat tcgggatcga attccatacc ctgtcgagca
      361 aaaaaatcgg aaagatgctg catttggttg aattcagctt cgaccacaca acgcgaggct
      421 ccctctttga taagccgtaa atcggctctc tgcccaagga tcagggaaat ggctcccatc
      481 aataccgatt tacctgcccc ggtttcgcct gtgataacgg aaaatccgga ttcaaattgt
      541 aaatccaaat gttcgatgag tacgtaattt tctatgtgga gcctggacaa catacaatca
      601 gttttgattc ttaatggcct gtaaacggtt gctttgtccg gggtatataa aactaagcag
      661 gtcgaagacc tcttgttttt ctttgctttc ggctttggaa taaagctgta tccattcttc
      721 gattttagaa tcggccaaat tgctcagtaa tggtgagctg gggttcaatc tttttacttc
      781 ttccagcacc ttgaaagagt cggtgatttt tttacgagcg gccgcagcat cctgatgcaa
      841 aagatccatg ccctggcggt ggtagttata attaagcagt cgcatcggtt tttgattttc
      901 atcgttcaag gcattggtcc agccccagca gtttttatcg tcttcgttcg ccttccagtt
      961 atcgcccaaa aaaccctgag cctgagatac tatatcactg gccttggtca aaaaccgttc
     1021 gccgccgtag ggagcgaaac tgtcaaaatc catgcaaaga acgatgtagg tccaaaaagc
     1081 cagggtggcg gttaaattgc tttgcagatt ggtttcgttg aattccagtt gttgattttc
     1141 caggtattca aattccaaat tttgccggat attgagcagg ctggttgtgt aagaagagcc
     1201 gaaaaccggc cttctcgatt gtatctgaag ctccgcctta tgtacgttat tctcactgct
     1261 ttgtatgatg aacaaaaggt tgcaatcgat tttttcgttt tgggctatag gagcgttcga
     1321 ccattgcctt tcgttcataa aggcggtaac tgcgtttcgc aggctttcgt agacagcctt
     1381 gtcggtaccc cctatctgat cggaattaac ctgcacccgg cagttcaatt cctgagccaa
     1441 agccaatccc ctgcccgata aaaaaaacag aaaaagaaaa acaaaactta tatgtatgtt
     1501 tttaaaagaa ttcacagctt agtaattaga tggtttacaa tgtcttcggc caccttctgt
     1561 ttgcttttga gcgggaaatc caacactttt cctccgggtt ccaaaatctt gattttattg
     1621 gtatcggctt tgaaaccggc cccttggtcg ttgagagaat tcaatacgat gaaatcaagt
     1681 ttcttccgct ccattttggc tcgggcattt tcaatttcgt tgtctgtttc caaagcaaag
     1741 ccaaccaaca cttgtccggg ttttttcacg ttccccaggt ttaaagcaat gtctttggtc
     1801 ggtttcaacg agaggccgaa gccttctttg ttgctcttga ttttagaagg ccggggattt
     1861 tcgggactga agtcggccac cgcggcacat aaaatcgcga catcgacggc ggggaaatag
     1921 tgcatacagg cctcgtacat ctcagcggca gtttctacgt cggtacggtg tatggcagga
     1981 tgatgggctt gaagcgatac cggcccggaa atcaactcca cctgagctcc tctattggcg
     2041 caggcttcgg ccagtgcgta ggccatttta ccggaggaat agttgcctat aaaacgtaca
     2101 gggtcaattt tttcgtaggt ggggcctgcg gtaatcaaaa cttttttgtc tttcaaatca
     2161 agttgagcgg aaaaaaaacg ttcgagttga gcgacaattt gttcaggttc ttccatgcgg
     2221 cctttgcctt cgagaccgct ggccagttct ccgctggcag gctctattat gtgattgccg
     2281 taacgtttga gcaattccag gttgtgcgtg gtggaaggat gccggtacat atccaagtcc
     2341 atagccggag ccacaaacac cggagctttc atcgaaagat aagtcgttat cagcatattg
     2401 tccgcaatgc cgttggccat
//
LOCUS       DAOSTD010000147         2418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_39786_length_2418_cov_9.502751, whole genome shotgun sequence.
ACCESSION   DAOSTD010000147 DAOSTD010000000
VERSION     DAOSTD010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39786_length_2418_cov_9.502751"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PLI37_07570"
     CDS             <1..988
                     /locus_tag="PLI37_07570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ58904.1"
                     /translation="FAKANNLAIEASESDYVLLLNPDTLVQEESIRNMLAFMDAHPEA
                     GALGVKMIDGRGRFLPESKRGLPTPTVSFYKISGLSALFPRSRRFGRYHLGWLDKDKI
                     HSVEILAGACMLVRREAIAKAGMLDEDFFMYGEDIDWSYRILKAGYRNYYFPPGRILH
                     YKGESTKKGSLNYVYVFYHAMAIFAKKHFLGKNFFYALLINLAIWLSGAFSFFSGLLR
                     KSLYIIKKTLSSTQQASVLVWGSAPEFERVRDLYSVGSSGSLNFIQASNREQVEETLK
                     DKDIRELVFCTAELEYSSVFDCMEKYAGRGLVFKMAPPIGQVIIGSHTILKS"
     gene            1083..1625
                     /locus_tag="PLI37_07575"
     CDS             1083..1625
                     /locus_tag="PLI37_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HOQ58905.1"
                     /translation="MSKTNEQFDAVTAKCRSIFSKKLHDYGAAWRILRIPSVTDQLFI
                     KANRIRNIEIKGESKIQDNIADEFMGIINYAIIGLIQLQQGSSEKVDITPEEALALYD
                     EKMEMARQLMLNKNFDYEEAWRGMRISSYTDLILMKINRVKQIEDLGGLTIASEGIDS
                     NYLDMLNYAAFGLIRLGYDK"
     gene            1629..>2418
                     /locus_tag="PLI37_07580"
     CDS             1629..>2418
                     /locus_tag="PLI37_07580"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOQ58906.1"
                     /translation="MKKGHKILALISRLLLGITFVFSGFVKAVDPLGSTYKIQDYLQA
                     FGLGFFDQYALFFSFVLFCLEFVLGMALLLGAAQRFFAYLALVFISLMTLLTLVIAIG
                     NPVSDCGCFGDALVLTNWQTFAKNIFLLLLSLTLVSFKGPLYKVFGEKTQKWACMVCL
                     LMVILVAVHSLRHLPMLDFRPYKTGNSIPELMQIPPGAPRDSVVMEYVYEKEGVYRSF
                     GLENYPAGDTTWHFVDRKERIVRKGYEPPIHDFVLLHPVYGDITE"
BASE COUNT          604 a          514 c          571 g          729 t
ORIGIN      
        1 ttttgccaaa gccaacaatc tggccatcga agcctccgaa tccgactatg tacttctgct
       61 aaaccccgat accttggtgc aggaagaatc tattcgcaat atgctggcct ttatggatgc
      121 acatccggag gccggcgccc tgggagttaa gatgatagac ggacggggcc gttttttgcc
      181 ggaatccaaa agaggcttac caacgccgac ggtttctttc tataagattt cgggtttgtc
      241 tgccttgttc ccccgttccc ggcgcttcgg acgttaccat ctgggctggc tggataaaga
      301 taaaattcat tcggtcgaga ttttagccgg tgcctgcatg ttagtccgca gagaagccat
      361 tgccaaggcc ggtatgctcg acgaagattt ttttatgtac ggcgaagaca tcgattggtc
      421 ctatcgcatt ttgaaagccg gctaccggaa ttattatttc cctcccggcc gcatacttca
      481 ctacaagggc gaaagcacca aaaaaggcag tctgaattat gtctatgtct tttaccatgc
      541 gatggctatt tttgccaaaa aacacttttt gggtaaaaac tttttttacg ccttgctcat
      601 caatcttgct atttggttga gcggagcttt ttcttttttc tcaggcctgc tgcgaaaatc
      661 tctgtacata attaaaaaaa ctcttagctc gacgcagcag gcttctgtgc ttgtttgggg
      721 tagtgccccc gaattcgaga gagtgcgaga tttgtacagc gtcggttctt ccggatcact
      781 gaattttatt caggcttcca accgggagca agtggaggag accttgaaag acaaggacat
      841 cagggagctg gtgttttgta cggcagagct ggaatattcc tccgtgtttg attgtatgga
      901 aaaatatgcc ggcaggggcc tggtcttcaa aatggcaccg cctatcgggc aggtcataat
      961 aggtagtcat acgattttaa agtcctgatt ttttacaagt cttttatttt gaccgacccg
     1021 aaaagttggt ttttatttct aaaaatcagt aattttgtca ctcacccgaa acacctcact
     1081 ctatgagtaa gaccaacgaa caattcgatg ccgtaaccgc taaatgccgc agcattttct
     1141 caaaaaagct gcacgattac ggagccgcat ggcgtatttt gcgcatacct tccgttaccg
     1201 atcaactttt tatcaaagcc aatcgcattc gtaacatcga aatcaaaggc gagagcaaaa
     1261 tacaagataa tattgccgac gaatttatgg gtattataaa ttacgccatt atcggtctta
     1321 ttcagttgca gcaaggttct tcggaaaagg tggatattac tccggaagaa gcgctggctt
     1381 tgtatgacga aaaaatggaa atggccaggc aattgatgct caataaaaat tttgattacg
     1441 aagaagcctg gagaggtatg cggattagtt catataccga tctgattctg atgaaaataa
     1501 acagggtgaa gcaaattgag gatttgggtg gcctaaccat cgcttccgag gggatcgact
     1561 ccaattacct cgatatgttg aattacgctg cctttggtct gattcgtctc ggttacgata
     1621 aataaaatat gaagaagggg cacaaaattc tggctttaat ttcaaggttg cttttaggaa
     1681 taacttttgt gttttccggc tttgtcaagg ctgtggatcc cttgggcagc acctataaaa
     1741 ttcaggatta tctgcaggct ttcggtttgg gcttctttga tcaatacgcc ctgttttttt
     1801 cctttgtgct tttctgcctt gaatttgtgc tgggtatggc cttgttgttg ggggcagcac
     1861 aaagattttt tgcctacttg gctctggtgt ttatctcgct gatgacttta cttaccttgg
     1921 tgattgcaat aggaaatccg gtgagcgatt gcggttgttt cggagatgcc ttagtgttga
     1981 ccaactggca aacctttgcg aaaaatattt tccttctttt gttgtccctc accttggtca
     2041 gtttcaaagg gcccctatat aaagtgtttg gagaaaagac gcaaaaatgg gcctgtatgg
     2101 tttgcctgct gatggtgatc ctggttgccg ttcattcctt gcgtcatttg cccatgttgg
     2161 attttcgtcc ctataaaaca gggaattcca tccctgagtt gatgcagatt cccccgggag
     2221 ctccccgaga ttcggtggtg atggaatacg tttacgaaaa agaaggggtt tatcgatctt
     2281 tcggtttgga gaattatccg gccggagaca ccacctggca ttttgtcgat cgcaaagaga
     2341 gaatcgttcg gaaaggttat gaacctccca ttcacgattt tgtattactt catccggttt
     2401 acggagatat taccgaga
//
LOCUS       DAOSTD010000148         2410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_39969_length_2410_cov_14.138854, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000148 DAOSTD010000000
VERSION     DAOSTD010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39969_length_2410_cov_14.138854"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1976
                     /locus_tag="PLI37_07585"
     CDS             <1..1976
                     /locus_tag="PLI37_07585"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOQ58907.1"
                     /translation="LLSKIDSIRFVSSPQAGFWIHKTDSLIFYADRQVDSLVFFEWSA
                     SECLRRFEVPTINIQVEGNAPVLSKDKADYLNCTVEIDGKEMYDDYRGSARIRGRGNS
                     TWFWYDKKPYRIKLDKKSKILGLGSNKDWVLLANYRDPTHLMNAFGFEMASWLGLPFT
                     NHTRYVEVTLNGDYIGLYQLTEQVEQAGDRVDIDVDEGVLLSLDKDDGPELAPNAVDN
                     FWSAYYRLPICVKHPKNPDEETKARIKSDFQELELLIKQCKYRDLSRRFDIPSLIDYL
                     IVQEMVYNVEMEAPRSVYMYKDTDEVYRMGPVWDFDAGFDFDWSTMTTGHNYFMAQEL
                     VLGTDPFRHTRARNLSPFFTQMFDNTLFVQQFKERWAETQPECLDYCLRMMDDYAAHT
                     AAAMARDFERWPIDKNYAEEIVKMKNWLTDRLDVLTEVIEKYPPGNIPEDTPESLFRE
                     ITDLLADIAAWLSETDFEGKATLESAYQQVLELQLSENNSIEELQTSLDFLNEALTNY
                     LAGNIEENLALTATLTTSHCSSWESLEAVRDGLVPENSSDRTYPRYGNWDGRQGVLHW
                     VQYEWEQACTVSEIGVYWWTDFGGLLQPETAYVEYWSAGNWIKLGDIGREFDQFNVLD
                     NLNVQTTKVRLSMSSKIATGISEFYVRGFRSKD"
     gene            2087..>2410
                     /locus_tag="PLI37_07590"
     CDS             2087..>2410
                     /locus_tag="PLI37_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58908.1"
                     /translation="MKTKLLMLTLFCLSIGLQQVQAQATQDVTETYIVNPSFEEGDGE
                     AEPTYWSFDSAGVFKGWYVSHPAITYNNNDADATKTGTYILGIWGNSADYEVSQIIKD
                     LPAGTY"
BASE COUNT          656 a          547 c          594 g          613 t
ORIGIN      
        1 atcttttgtc taagatcgat agcattcgtt tcgtaagcag tccacaggcc ggtttttgga
       61 tacataagac agacagcctg attttttatg ccgatcgtca ggtggacagt ctggtgtttt
      121 ttgaatggtc tgcctccgag tgtctcagac gatttgaagt tcccaccatc aatattcagg
      181 tggagggtaa cgctcctgtt ttatccaagg ataaagccga ttacctgaat tgcacggtag
      241 agatagacgg caaagaaatg tacgacgatt accggggctc tgcccgtatc aggggccggg
      301 gcaattcaac ctggttttgg tacgacaaaa aaccttaccg catcaagttg gataaaaaat
      361 caaaaatact ggggttgggt tccaacaaag attgggtttt gctggccaat taccgcgacc
      421 ctacccatct gatgaatgct tttggatttg aaatggcctc ctggcttggt ttgcccttta
      481 ccaatcatac ccgttatgta gaagtaactc tcaatggcga ctatataggt ttgtaccagt
      541 tgaccgagca ggtggagcag gccggagacc gggtagatat agacgtcgac gaaggagtgc
      601 tgctttcgct cgacaaagac gacgggccgg aactggcacc caatgccgta gataattttt
      661 ggtctgccta ttatcgtctg cccatctgtg tgaaacatcc caaaaacccg gacgaagaaa
      721 caaaagcccg gattaaatcc gattttcagg aattggaact cttgatcaaa cagtgcaaat
      781 acagagattt gtcccggagg tttgatatac cctcgttgat tgattatctg attgttcagg
      841 aaatggtcta caacgtcgaa atggaggcgc cgcgcagtgt ctatatgtat aaggataccg
      901 atgaggtgta ccggatgggg ccggtctggg atttcgatgc cggtttcgat ttcgactgga
      961 gcaccatgac cacagggcat aactacttta tggcgcagga attggtactg ggaacagatc
     1021 ctttcaggca tacccgggcc cgtaatctgt cgcctttttt cacgcaaatg ttcgacaaca
     1081 ccttgtttgt tcaacaattc aaagagcgat gggccgaaac ccaacccgaa tgtctcgact
     1141 attgcctgag gatgatggac gattatgccg ctcatacagc cgcagccatg gccagggatt
     1201 ttgaacgttg gcccatcgat aagaattatg ccgaagaaat tgtaaagatg aaaaattggt
     1261 tgacagatcg cctcgatgtg ttgaccgaag tgatagaaaa gtatcctccc ggaaatatac
     1321 cggaagatac tcccgaaagc ctgttccggg aaatcacaga cttgttggca gatattgccg
     1381 cctggttaag tgaaacggat tttgagggta aagcaaccct ggagtctgct tatcaacaag
     1441 tcttggaact ccaactctcg gaaaacaaca gtatagaaga attgcagaca agcctggatt
     1501 ttctcaatga agctttgacc aactacctgg cgggcaacat tgaagagaac ctggccctga
     1561 cggcaacgct caccacctcg cactgctcga gttgggaaag cctcgaagcg gtacgcgatg
     1621 gtctggttcc ggaaaactcc agcgacagga cctatcccag atacggaaac tgggacggcc
     1681 gtcagggcgt gttgcattgg gtgcagtacg aatgggaaca agcctgtacg gtatcggaaa
     1741 tcggagtcta ctggtggacc gatttcggcg gtttgctgca gcccgagacg gcttatgtgg
     1801 aatattggtc tgccggtaat tggatcaaac ttggagatat cggcagggaa ttcgatcaat
     1861 tcaatgtatt ggataatctg aacgtacaaa ccaccaaagt ccgtttaagt atgtcgagca
     1921 aaattgccac ggggatatcg gagttttatg tcaggggttt ccgctccaaa gactaaagat
     1981 tggactattg tacgacttaa ttggattttt cttttatttt taatttggca ttgttcctat
     2041 ttttgttgct gatcattcat tttttttaaa ctactcctaa acgcctatga aaacaaaatt
     2101 actcatgctt acgcttttct gcctaagtat tggacttcaa caagtccaag cccaagccac
     2161 gcaagatgta accgaaactt acatcgtgaa cccgagcttc gaagaaggtg acggagaagc
     2221 cgaacccacc tactggagtt tcgactccgc cggggtgttt aaaggctggt acgtcagcca
     2281 tccggccatc acctacaaca acaacgatgc cgacgccacc aaaaccggaa cctatatcct
     2341 cggtatttgg ggtaattcgg ccgattacga agtatcgcaa atcataaaag atcttccggc
     2401 cggaacctat
//
LOCUS       DAOSTD010000149         2400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_40205_length_2400_cov_17.888699, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000149 DAOSTD010000000
VERSION     DAOSTD010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40205_length_2400_cov_17.888699"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..794)
                     /locus_tag="PLI37_07595"
     CDS             complement(108..794)
                     /locus_tag="PLI37_07595"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOQ58909.1"
                     /translation="MTILYEDNHLIIVNKSCSEIVQGDKTGDPSLEEELKQWIKQTYH
                     KPGNVFLGVTHRLDRPVTGVVIFAKTGKALARINEMFRKGEIQKTYFALVKNSPPAIE
                     GRLEHFLWRNEKQNKSYPCKQEKPGSKKAILDYKLLGRSDRYFLLEIDLKTGRHHQIR
                     SQLAGIGCPIKGDLKYGYPRSNPNKGISLHAASVRFIHPVSKIELNIKAPFPEDEALW
                     AVFKQQGCLE"
     gene            complement(802..1548)
                     /gene="fabG"
                     /locus_tag="PLI37_07600"
     CDS             complement(802..1548)
                     /gene="fabG"
                     /locus_tag="PLI37_07600"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOQ58910.1"
                     /translation="MKLLEGKTALITGAARGIGKAVALRFAQEGANIAFTDLVLNEQV
                     LETEAEIAAYGVKVKAYASDASNFEETHRVVDDIVKEFGKVDILVNNAGITKDGLMMR
                     MSEAQWDAVININLKSAFNFIHALTPVMMKQRGGSIINMSSVVGVSGNAGQANYSASK
                     AGMIGLAKSVAAELGSRGIRANAVAPGFIITEMTGQLPEDVRKAWCEGIPLRRGGSPE
                     EVANVCVFLGSELSSYVSGQVIHCCGGMNR"
     gene            complement(1720..1971)
                     /gene="rpsT"
                     /locus_tag="PLI37_07605"
     CDS             complement(1720..1971)
                     /gene="rpsT"
                     /locus_tag="PLI37_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOQ58911.1"
                     /translation="MANHKSALKRIRQAQARRLRNRYYAKTTRNAVRDLRATTDKAEA
                     EKMFPGVVSMLDRLAQKGIIHKNKAANLKSKLAKSVAKL"
     gene            complement(1994..2065)
                     /locus_tag="PLI37_07610"
     tRNA            complement(1994..2065)
                     /locus_tag="PLI37_07610"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2030..2032),aa:Glu,seq:ttc)
BASE COUNT          572 a          534 c          589 g          705 t
ORIGIN      
        1 atattaaaaa atcaagccgc aagagtgaat tttttaattt cctggtagtt gtaattcgct
       61 gtatttcagc tttaaaaaat aaaaaatcaa gaagagtgaa ggtcgttcta ttccagacag
      121 ccttgttgtt taaaaaccgc ccaaagcgcc tcgtcttcgg ggaaaggcgc cttaatattg
      181 agttcaattt tgcttaccgg gtgtataaaa cgtacacttg cagcatgcag cgaaatgccc
      241 ttgttgggat tggagcgcgg gtagccgtat ttaagatcgc ctttgatagg gcagccaatg
      301 ccggccaatt ggctgcgaat ctgatgatgc cggccggttt taagatcgat ttcgagtaag
      361 aaatagcgat cggagcgccc caggagctta taatctaaaa tcgccttttt cgatccgggt
      421 ttttcctgct tgcagggata acttttattt tgtttttcgt tacgccataa aaaatgctcc
      481 aaccggccct caatagcggg agggctgttt ttgaccaggg caaaataagt cttctgaatt
      541 tcccccttgc gaaacatttc gtttatacgg gccagcgctt tgccggtttt ggcaaagatc
      601 acaacccccg taaccggtcg atccaatcta tgggtcacgc ccaaaaagac attgccgggc
      661 ttgtggtagg tttgcttaat ccattgcttg agctcttcct ccagcgaggg gtcgcctgtt
      721 ttgtcgccct gaacaatttc agaacaagac ttattgacaa taatcaggtg attgtcttcg
      781 tagaggatag tcataaagcg tttagcggtt cattcccccg caacaatgga ttacctgtcc
      841 gcttacatac gaagaaagct ctgagcccag gaaaacacaa acattggcca cctcttcggg
      901 gctgcccccg cggcgaagcg gaataccttc gcaccaggct ttgcgtacgt cttcgggtaa
      961 ttggcctgtc atttcggtga taataaagcc gggagcaacc gcattggcgc gtatgcccct
     1021 ggaaccaagc tcggcagcca ccgatttggc caggccgatc atgccggctt tggaggccga
     1081 atagttggct tgtccggcgt tgcccgatac acctaccacg gaactcatat tgataatgct
     1141 gcctccgcgt tgtttcatca ttaccggggt aagggcatgg ataaaattaa aggccgattt
     1201 caggttgata ttgatgacgg cgtcccattg ggcttcgctc atgcgcatca ttaagccgtc
     1261 tttggtgata ccggcgttgt tgaccaggat atctactttc ccaaattcct ttactatgtc
     1321 gtcgaccacc cggtgggttt cttcaaaatt ggaagcgtcg gaagcgtagg ctttcacttt
     1381 gactccgtag gctgctattt cggcttcggt ttctagaact tgttcattca aaaccaaatc
     1441 ggtaaaagca atgttggcgc cttcttgtgc aaaacgcaat gcgactgctt tgcctattcc
     1501 tctggcagcg ccggttatta aagcggtttt tccttctaat aatttcataa tcgtgttatt
     1561 tggttaaggg taggaggcaa gctgtaaatg taaagcttac gtccttgctg agaattttgc
     1621 aaaagtaaga ttttcttgtg gtttagcaag caaagggcat aaaaaagacc acaggaaatc
     1681 tgtggtctga cttatgaaga cggcaagtgg agaagctttt tagagcttag ccacgctttt
     1741 tgccagctta gattttaaat tggcagcttt gttcttgtgg ataattcctt tttgagccaa
     1801 acgatccaac atggacacaa cgccgggaaa cattttttcc gcttcggctt tgtcggtggt
     1861 ggcacgcagg tcgcgaacag cgttacgggt ggttttggcg tagtaacggt tgcgcaggcg
     1921 tctggcctgt gcctggcgta ttcttttgag ggctgatttg tggtttgcca ttgttgtttt
     1981 cttaagtgtt ttgtagccca tagcggaatc gaaccgctct ttcaagaatg aaaatcttgc
     2041 gtcctaaccg atagacgaat gggccagcct gtggcaaaaa agcggttcgg ccattgccgg
     2101 cgctaatttt gcggctgcaa agataagacg attttatttt ccggcaaaag tttttcagga
     2161 aaaattttat cggagcgcat ataattttga aatcgaccga ttatcggttt tgcgtggggt
     2221 tgtcattacg cattcgggag cgcaggtgtc gtcaaagagc tgcttgctgt ttcgaagctt
     2281 gcagaattgc ttcgtagctt tctctgttca tcagcaaatc gagagccttc gatacgcatt
     2341 catctgtttt gagcgattcc tgaatttctc ctttttgata atagtagcgc ctgatgattt
//
LOCUS       DAOSTD010000150         2384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_40575_length_2384_cov_16.753113, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000150 DAOSTD010000000
VERSION     DAOSTD010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40575_length_2384_cov_16.753113"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1385
                     /locus_tag="PLI37_07615"
     CDS             156..1385
                     /locus_tag="PLI37_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOQ58912.1"
                     /translation="MNDDIYTLPNQIRVIRINCQADVSCCGFAIAAGSRDEEEKEQGL
                     AHFTEHLLFKGTHKRKAFHVINRLNAVGGELNASTGKEETFVYGLFLKNDFERALELL
                     CDMLFHSTFPQREVEKEREVVLDEINSYEDSPSELIFDDFERCLFEHHPFGRNILGAP
                     ECLRAFGSSDLKAFARRCYTTDRLVFFSLSSLENKKTDALVQKYLAQIPDNSADSVLN
                     LRSSPQPVKAFKKLFSRNTHQAHVLYGNTAYTMFDEKRYALFLLNNLLGGPGMNSRLN
                     TLLREHSGLVYTVESGLSFYTDTGLCSIYFGSDPKNTDRCLSLIDKELKQLREQPLSS
                     SKLHYAKKQLFGQLSIASENKESRALNMGKSLLYYNKYQSLDDFMAKIEAVDAMQLCE
                     VASEVFNPSEMSLLVYS"
     gene            complement(1368..2297)
                     /locus_tag="PLI37_07620"
     CDS             complement(1368..2297)
                     /locus_tag="PLI37_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOQ58913.1"
                     /translation="MRKIIGIGESILDIVFKNRQPVSATPGGSVFNGLISLGRLGLPV
                     YFVSDIGQDQVGRFITDFMENNGLSSKYMNLHPDTNTAVALAFLDENNDANYTFYKDY
                     SVKGLQGNFPPINTDDIVIFGSYYALNPLLRKRVKAFLEKAVQKSALIYYDINFRKSH
                     SYQAKALNPVLIENYRYADIIRGSEDDFKALYQKQHAGGIYREKIAPYCENFIFTAAE
                     KNVEFYCPRFTASFPVKKIPTLSTVGAGDSFNAGILYGLMQSGICRKDLALLNQSDWE
                     KIIALGIDFSAKVCQSYENYIPRPESSNSGVNQ"
BASE COUNT          633 a          541 c          534 g          676 t
ORIGIN      
        1 cttatacacc ctaaaaaaca aagactggag gaattgttgc ccggtcctta tgtacaggtc
       61 ttcgaccgga aattcggttt tttacccgat ttatccgtgc ttgatctttt gttcaacacc
      121 ggtccggagg cctgtttatt tttaatgcat aagcaatgaa cgacgacatc tatactttgc
      181 ccaatcaaat acgtgtaatt cgtataaatt gccaggccga tgtttcttgt tgcggatttg
      241 ccattgccgc aggttctcgc gacgaagaag aaaaagagca ggggttggcg catttcacgg
      301 aacatttatt attcaaaggc acgcataaac gtaaagcctt tcatgtaatc aaccgcctaa
      361 atgccgtggg cggagaattg aatgccagca ccggcaagga agaaaccttt gtttacggtc
      421 tttttctcaa aaacgacttc gaaagggcgc tggaactttt gtgcgacatg ctttttcatt
      481 ccacttttcc ccaaagagaa gtcgaaaaag aaagagaagt ggtgctcgac gaaatcaata
      541 gctacgaaga ttctccttcg gaactcattt tcgatgattt tgagcgttgt ctgtttgaac
      601 atcacccttt tgggcggaat attctgggtg caccggaatg tctgcgggca ttcgggtctt
      661 ccgatctcaa ggcttttgcc cggcgttgtt acaccaccga ccgtctggtc tttttttctc
      721 tgtcttccct ggaaaataaa aaaacggacg cccttgtgca aaagtacctg gcccaaattc
      781 ccgacaattc ggccgattcg gttcttaatt tacgcagcag tcctcagcct gtaaaggctt
      841 tcaaaaaact tttttccaga aatactcatc aggctcatgt tttgtacggc aatacggctt
      901 acaccatgtt cgacgaaaaa cgttatgctt tgtttctgct gaacaacctg ctgggcggcc
      961 cgggcatgaa cagccggctt aatactttgc ttcgtgagca cagtggcctt gtgtacactg
     1021 tagaatcggg gctcagcttt tacaccgata ccggcctgtg ttccatctat tttggcagcg
     1081 atcccaaaaa caccgatcgt tgcctgtcgc tcatcgataa agaattgaaa caattgcgag
     1141 agcagccttt gagttcgtcc aaattgcatt acgcaaaaaa acagctcttt ggccagttga
     1201 gcatagcttc cgaaaataaa gaaagcaggg ctttgaatat ggggaaaagc ctgctttatt
     1261 acaacaaata ccagtccctg gacgatttta tggccaaaat cgaggccgtc gatgccatgc
     1321 agctttgcga ggtggcatcc gaagtgttta atccctctga aatgtcttta ctggtttact
     1381 cctgaatttg aactctccgg cctgggaata taattttcgt aggattggca gacctttgcc
     1441 gagaaatcta tgcccaaagc gataatcttt tcccagtcgc tttggttgag caaggccagg
     1501 tctttgcggc aaatacccga ttgcataagt ccgtaaagta tacctgcatt gaaagagtcg
     1561 ccggcgccca ctgtgctgag ggttgggatc ttcttgacgg ggaagctcgc cgtaaatcgt
     1621 ggacaataga actccacatt tttttcggct gccgtaaaaa taaagttctc gcaatagggg
     1681 gcaatttttt cgcgatagat accgccggcg tgctgttttt ggtacaatgc cttgaagtcg
     1741 tcttccgatc ccctgataat gtcggcgtag cggtaattct ctatcaatac gggattcaag
     1801 gctttggcct gataactgtg agattttctg aaattgatgt cgtaataaat caaagccgat
     1861 ttttgtaccg ctttttccaa aaaagctttc acccgtttgc gcagcaaagg attcagggca
     1921 taataagaac cgaaaataac tatatcgtcg gtattgatgg gagggaaatt gccctgtagg
     1981 cccttaactg aataatcttt gtaaaaagta taattagcgt cgttattttc gtccagaaag
     2041 gccagggcaa ctgcggtgtt ggtgtcggga tgcagattca tatatttgga agacaggccg
     2101 ttgttttcca tgaaatcggt gatgaacctc cccacctggt cctgacctat atcgctgaca
     2161 aaatacaccg gaagacccag ccttccgagc gaaatcagcc cgttgaatac tgaaccgccg
     2221 ggagtggccg acacaggttg cctgtttttg aatacgatat cgaggatgct ttctcctata
     2281 ccaattattt tgcgcataat tttttaactc aacgaacaaa gatactcgaa aatagaaata
     2341 aaagtattac ctttgcggcc actattttct aattacttaa atta
//
LOCUS       DAOSTD010000151         2383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_40605_length_2383_cov_15.024914, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000151 DAOSTD010000000
VERSION     DAOSTD010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40605_length_2383_cov_15.024914"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PLI37_07625"
     CDS             <1..1203
                     /locus_tag="PLI37_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOQ58914.1"
                     /translation="HNIHASVSTYNIRPTDMPKGIYTDVKGNEATAWGLIAAAQQAGL
                     QLFLGSYPITPASEILQELAARKDLGVITVQAEDEIAGICTAIGASFAGNLAATSTSG
                     PGLALKSEGINLAVMAELPLVIIDVQRGGPSTGLPTKTEQSDLLQALYGRNGESPLVV
                     LAASTPSNCFDFAYMASKLALEHMTPVILLTDGFLANGSSAWRLPDLSQFPAIKPPFV
                     HKEMQGKWAPYLRNEQGSRYWAVPGTEGFAHRIGGLEKDSRTGAISTDPANHQLMTDL
                     RQQKIDNIANVIPPLQLSGNSNADLLVVGWGGTYGHLYSAVRQFNEQGGQAALAHFNY
                     INPLPANTEEVLNKFKKIVVCELNSGQFASHLRSKLKLKGEILQYNKVQGQPFTVEEL
                     LGHFSTIL"
     gene            1214..2224
                     /locus_tag="PLI37_07630"
     CDS             1214..2224
                     /locus_tag="PLI37_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOQ58915.1"
                     /translation="MQTSYTVNDFKSDQYVRWCPGCGDHALLNSIQRAMAEIGVSPDN
                     TAVISGIGCSSRLPYYVNTYGFHTIHGRASAIATGVKVANPRLAVWQITGDGDCLAIG
                     GNHFIHAVRRNVDINIVLVNNGIYGLTKGQYAPTSKRGFISKSSPYGTIEDPFNASNL
                     CLGAGGQFFARILDTDLKNSQEVLKLAAQHKGCSVVEALVNCVIFNDGTHKEVSAKEV
                     REDHTIFLRHGEPMIFGKDRDKGLIVDGFKLKVVKIGENGITENDILVHDAKCPDKTL
                     HFKLGHMRGPELPLALGVIRDTEAPVYDEEVYTQFEEIKPKRNYKSLNDFFMSLETWE
                     IK"
BASE COUNT          618 a          657 c          553 g          555 t
ORIGIN      
        1 cacaacattc atgcatccgt gtcgacctac aatattcgtc ccaccgacat gcccaagggc
       61 atttataccg acgtcaaagg caacgaagca acggcctggg ggctgattgc cgctgcccaa
      121 caggccggac tgcaactgtt tttgggcagc tatcctatta ctccggcttc ggaaatcctt
      181 caggaactgg ctgcccgcaa agatttgggt gtcattaccg tacaggccga agacgaaatt
      241 gccggaatct gtacggccat tggagcctct tttgccggta atctggcggc aacctccact
      301 tccggccccg gactggcctt gaaatccgaa ggcatcaacc tggctgtgat ggctgaattg
      361 cccctggtca ttatcgatgt gcaacgcgga gggccttcca ccggattacc gaccaaaacc
      421 gaacaatccg acttgctgca agctctttac ggccgcaacg gagaatcacc tctggttgtt
      481 ttggccgcaa gtaccccttc caactgcttc gatttcgctt acatggcctc caaactggct
      541 ctggaacata tgactccggt tatcttgctg acagacggtt ttctggccaa cggttcctcg
      601 gcctggagat tgcccgatct cagccaattc ccggccatca agcctccttt cgttcacaaa
      661 gaaatgcagg ggaaatgggc tccttatttg cgtaacgagc aaggttcacg ttattgggcc
      721 gttcccggga cagagggttt tgcacaccgc ataggcggcc tggaaaaaga ttcccgtacg
      781 ggagccattt ccaccgatcc ggccaatcac caactgatga cagatttgcg tcagcaaaaa
      841 atcgacaata ttgccaacgt gattcctcct ttgcaactga gcggaaacag caacgccgat
      901 ttgctggtgg tcggttgggg aggcacatac ggccatttgt attcggccgt gcggcaattc
      961 aacgagcagg gcgggcaagc cgcgctggct cacttcaatt atatcaaccc cctgccggcc
     1021 aataccgaag aagttttgaa caagttcaaa aagatagtgg tctgcgaact caactccgga
     1081 cagttcgctt ctcacctgcg ctccaaactg aaactcaagg gagagatact tcaatacaat
     1141 aaggtgcagg ggcaaccctt taccgtcgaa gagctgctcg ggcatttttc aaccatacta
     1201 taacaaacaa gtcatgcaaa catcctatac agtcaacgat tttaaaagcg accaatacgt
     1261 aagatggtgc ccgggctgcg gcgatcacgc gctgcttaat tctattcaga gagctatggc
     1321 ggaaatcggc gtctctcccg acaataccgc agttatttcg ggtatcggct gctcttcacg
     1381 acttccttac tacgtcaaca cctacggttt tcataccata cacggacgag cttcggccat
     1441 tgctaccggc gtaaaagtgg caaatccccg tctggccgtc tggcaaatca ccggcgacgg
     1501 cgattgtttg gccatcggag gcaatcactt tattcatgca gtccgcagaa atgtcgatat
     1561 aaacatagtg ctggtgaaca acggtattta cgggctgacc aaagggcaat acgctcccac
     1621 ctccaaacgg ggctttattt ccaaatcctc accatacggc accattgaag atccctttaa
     1681 tgcttccaat ctttgccttg gagccggcgg acaattcttc gcccgtattt tggatactga
     1741 ccttaaaaat tctcaggaag ttttgaagct tgccgcacaa cacaaaggct gttctgtagt
     1801 ggaagccttg gtcaattgtg ttatattcaa cgacggcaca cacaaagaag tcagcgccaa
     1861 agaggttcgc gaagaccaca ccattttcct gcggcacgga gagcctatga ttttcggtaa
     1921 agaccgggac aaaggcctga ttgttgacgg tttcaaactc aaagtggtta agataggcga
     1981 aaacggcatc accgaaaacg acatccttgt tcacgatgcc aaatgtccgg acaaaactct
     2041 gcacttcaaa ctcggccaca tgagaggacc cgaactccct ctggctctcg gcgttatccg
     2101 cgataccgaa gcccccgtct acgacgagga agtctatacc caattcgaag aaatcaaacc
     2161 taaacgcaat tataagtccc tgaacgattt ttttatgagt ctggaaacct gggaaatcaa
     2221 atgaaactgc gacaactttg cctgattggt ttatgtttct ggtgttttaa cctgaaatcg
     2281 gaacagcccg aaacttacac gccctacggc ctgcaaaaca atatgccctt gttttaccga
     2341 caggccaaag aggcaatcac tttttccatg gcctgggaac acc
//
LOCUS       DAOSTD010000152         2367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_40960_length_2367_cov_11.024654, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000152 DAOSTD010000000
VERSION     DAOSTD010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40960_length_2367_cov_11.024654"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..898
                     /locus_tag="PLI37_07635"
     CDS             74..898
                     /locus_tag="PLI37_07635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ58916.1"
                     /translation="MKKLFFFISVLLLTSQTHMMHSEEKTTFVLMETSMGKVKIKLYN
                     ETPKHRDNFIKLVNEGYFNNLLFHRVIKDFMIQGGDPESRNAPAGKQLGSGGPGYTIP
                     AEFVYPKYFHKRGALSAARTGDQVNPQKASSGSQFYIVWGQVYKASDIDQLEAQKINQ
                     AKNAYFQQLAGKQQSKIQQLYQANDQAGLQKLQNELIAQMEAHFEANPPQGLSPEQIK
                     AYTSLGGTPHLDNEYTVFGEVVEGLEVVGKIQSVETGTADRPKTDVVILSMQVIGN"
     gene            970..2139
                     /locus_tag="PLI37_07640"
     CDS             970..2139
                     /locus_tag="PLI37_07640"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOQ58917.1"
                     /translation="MTTVNLMYNVGSKDEHPDHTGFAHLFEHLMFAGSARVPDFDKAI
                     REASGENNAWTSRDVTNYYITLPSHKLDTALFLESDRMSSLNINQRSLEIQKQVVLEE
                     FKQRHLNQPYGDVSMLLRSLAYEGHPYSWPTIGKSTEHIEAFNLDDVHAFYRRHYNPD
                     NAVLAITGDFDFEHLVALSEQYFGAIAAAETDRPEFPVWPQQEGPRFLEVERPVPGQA
                     LYKLWRAHAVCEEEYTTCDLLTDALSLGNSSRLYRQLVKERQVFSQADIYVGGEGLPG
                     AGSILFSGKPVKGVSLEEAEKALEVCLNQIATEAPGQEELNKLINKHESAFLFQNTDA
                     RHLATQACWYEILGSADNYLQEPERLKNLSGEDLRSMAEKVFRPENCSTLYYRNI"
     gene            complement(2168..>2367)
                     /locus_tag="PLI37_07645"
     CDS             complement(2168..>2367)
                     /locus_tag="PLI37_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58918.1"
                     /translation="PKGLLNVRPIVPRHHKKGVFDVVWMRGRYEFYADERYETSLVFP
                     KMEKTGEGEERFLNTSVSPEF"
BASE COUNT          658 a          628 c          521 g          560 t
ORIGIN      
        1 tctttttttg ggttagtaaa cacaaaggta gtcagaatat tcatatcttt gcgggactta
       61 aaataattta aacatgaaaa aactgttttt tttcatttca gtactgctcc ttaccagcca
      121 aactcacatg atgcattccg aagaaaaaac aacttttgtc ctgatggaaa cttccatggg
      181 caaagtcaaa atcaagctct acaacgagac ccccaaacac cgggacaatt ttattaagtt
      241 ggtaaacgaa ggctatttca acaacctgct gtttcatcgc gtcatcaaag actttatgat
      301 ccagggcgga gaccccgaat cgcgcaatgc cccagccggc aaacaattag gttccggtgg
      361 gcccggatac acaattcctg ccgaatttgt ttaccccaaa tactttcaca agcgcggtgc
      421 tttgtcggct gcccgcaccg gagatcaggt gaatcctcaa aaagcctcct ccggctccca
      481 attttatatt gtctggggac aggtttacaa agcctcggac atcgaccagt tggaagctca
      541 aaaaatcaac caggccaaaa atgcttattt tcaacaattg gccggaaaac agcaaagcaa
      601 aatccagcag ttgtatcaag ccaacgatca ggccggtctg caaaaacttc agaatgaact
      661 gatagcccaa atggaagctc atttcgaggc taaccctccc cagggactca gccctgaaca
      721 aatcaaggct tataccagcc tgggcggcac tcctcatctc gacaacgaat acacggtctt
      781 tggcgaagtt gtcgaaggcc tggaggttgt cggaaaaata cagtcggtcg aaaccggcac
      841 tgccgaccgt cccaaaaccg atgtggtaat ccttagtatg caggtgatcg gaaactgact
      901 atgcgtttct tctcccaccg gctgagcaat ggtttgcgtc tcttgtactg ccctataagc
      961 aatacaagaa tgactactgt taatcttatg tataacgtgg gttccaaaga tgaacatccc
     1021 gatcatacgg gctttgccca tctttttgaa cacctgatgt ttgcaggctc tgccagggtg
     1081 cccgattttg ataaggccat acgggaagcc tcgggcgaaa acaacgcctg gaccagtcgc
     1141 gacgttacca attattacat caccctgccc tcgcacaagc tcgacacagc cctgtttttg
     1201 gaaagtgacc gcatgtcgtc gctcaacatc aatcaacgta gcctcgagat tcaaaaacaa
     1261 gtggtgttgg aagagttcaa acaacgccac ctgaatcaac cctacggaga tgtttccatg
     1321 cttttacgct cactggccta cgaggggcat ccttattcct ggcccaccat cggaaagtct
     1381 acggagcata tcgaagcctt caacctggac gatgtacacg cattttaccg ccggcattac
     1441 aacccggaca atgctgtgct ggccattacc ggcgattttg acttcgaaca tctggtggca
     1501 cttagcgaac aatactttgg agccatagct gcggcagaaa cagaccgacc tgaatttccc
     1561 gtctggcctc aacaagaggg cccccgtttt ctggaagtgg aacgccccgt tcccggccag
     1621 gcattgtata aactctggcg ggcacatgca gtttgcgaag aggagtacac cacctgcgac
     1681 cttctgaccg atgccctgtc cttaggtaat tcctcgagac tttatcgaca actcgtcaag
     1741 gaacgccagg tattcagtca ggcagatatt tatgtcggcg gcgagggact gcccggagcc
     1801 ggcagcattc ttttttcggg caaacctgta aagggcgtta gcctcgaaga agccgaaaaa
     1861 gccctggaag tctgtctcaa tcagatagcc acggaggctc ccggacagga agaactaaac
     1921 aaactgatca acaaacacga atcggctttt ttattccaaa acaccgatgc cagacacctg
     1981 gccacacagg cttgctggta cgaaatcctc ggttctgccg acaattatct acaggaaccc
     2041 gaaagattga aaaacttaag cggcgaagat ctccgctcca tggccgaaaa ggtattccgc
     2101 cccgaaaact gctctacatt gtactataga aacatctgat attcagattg ttcgggatat
     2161 tttcaatcta aaattccgga gaaaccgacg tgttgagaaa tctttcctcc ccttcaccgg
     2221 tcttttccat ttttgggaaa accagagagg tttcgtagcg ttcatcggca taaaattcat
     2281 aacggccacg catccagacg acatcgaaaa cccctttttt gtgatgacgc ggcactatag
     2341 gccttacatt cagcaagccc ttgggtg
//
LOCUS       DAOSTD010000153         2337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_41711_length_2337_cov_15.080193, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000153 DAOSTD010000000
VERSION     DAOSTD010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41711_length_2337_cov_15.080193"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1517)
                     /gene="rodA"
                     /locus_tag="PLI37_07650"
     CDS             complement(51..1517)
                     /gene="rodA"
                     /locus_tag="PLI37_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOQ58919.1"
                     /translation="MNSKINPISLWKAVDIQTIVIYLFLVIVGWLTVFSASYNYDSAV
                     LFDLNSTAGKQLIWIAVSLVTAFFILLIDAKFYYYLSYNGYIIVMFLLLLTLLIAPEI
                     KGSRSWLVFGPFSIQPAEFAKFITALTLARYLSDYGFNTRHPGSIAILFALLLIPMGL
                     IFLQKESGTALVFLAFLLVFFREGMSGVLLFAGACFLSYFVLGLRFGEQTLWEHTSLG
                     NFSVLLLIALVVSGLLLRYHKKDNKAWLIVLLGNVLPVMAALIWNRFTKWGFDIALLQ
                     LALLGLSVVYALYQSIRHNSRNPALIALFALLSVAFLYGIDYTFDRVLQPHQQTRILV
                     LLGTMDDPSGVGYNINQAKISIGSGGFWGKGFLNGTQTKLKYVPEQDTDFIFCTIGEE
                     FGFAGSVFLLLVYGYLLVRLIFMADRQHFAFARIYGYCVASLLFVHLLVNVGMVLGLM
                     PVIGIPLPFLSYGGSALLGFTILLFIFLRLDADNMRRH"
     gene            complement(1501..>2337)
                     /locus_tag="PLI37_07655"
     CDS             complement(1501..>2337)
                     /locus_tag="PLI37_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOQ58920.1"
                     /translation="KFACYGGYFFARQKLGCHSHPSPLNLPDAISASCNAYFAAAYRS
                     MIDHPQYSSVQEAYEHWRRLICSIGFGSRLGVDLPYEQSGLIPTTDYYNGIYGQRGWR
                     GATIISNGIGQGEVLATPLQMANYCAVICNKGWYITPHIVRQVHGMEADTSIIKKHVS
                     GIDPEHFEPIIEGMRGAVTSPMGTSKGVSIPGIEVCGKTGTAENPGVDHSIFICFAPK
                     DDPQICMLVFIENGGFGATWAVPMASLMLEKYLKGSIAPGRKWMETRLLNANLMNVEL
                     KD"
BASE COUNT          702 a          563 c          555 g          517 t
ORIGIN      
        1 tggaaggcat ccggaatgtg ggagtatgcc tgaggcttaa gtgatctggc ctaatgtcgc
       61 ctcatattgt cggcatccag ccgcaggaaa ataaacaaaa gaatagtaaa tcccagtaaa
      121 gcagagcctc cgtaacttaa gaaaggcaaa gggataccta ttacaggcat caggccgagc
      181 accatcccca cattcaccaa caaatgcaca aagagaagac tggccacgca atagccgtaa
      241 attcgcgcga aggcaaagtg ttgccggtct gccataaaaa tcagtcggac cagcaaataa
      301 ccatatacca gcagaagaaa taccgagccg gcaaagccga attcctcacc tatggtacag
      361 aaaataaagt cggtgtcctg ttccgggacg tatttcaatt tggtctgcgt tccattcagg
      421 aaacccttgc cccaaaaacc gcccgatcct atggaaatct tggcctgatt gatgttatag
      481 cccacccccg aaggatcgtc catggtgccc agcaaaacca gaatacgtgt ttgttgatgg
      541 ggctgcagca cacggtcgaa ggtatagtct ataccataca aaaaggccac tgagagcagg
      601 gcaaaaagag caatcaaagc cggatttctg gaattgtggc gtatgctctg gtacaaggcg
      661 taaacaacag ataagcccaa gagcgccaac tgaagcaggg caatatcaaa accccatttg
      721 gtaaagcggt tccagattaa ggctgccatt accggcaaaa cgttgcccag caaaacaatc
      781 aaccaggctt tgttgtcttt tttgtggtag cgaagaagca aaccggaaac aaccaaggct
      841 ataagtagta gtaccgaaaa attgcccaga gaagtatgtt cccacaaggt ctgttcacca
      901 aatctgagcc ccaacacaaa ataactcagg aaacaggcac cggcaaaaag caacacgccc
      961 gacatccctt cgcgaaaaaa gaccagcagg aaggccaaaa acaccaaagc ggtaccggat
     1021 tctttttgta aaaagatcaa tcccatgggg ataagtaaaa gagcaaacaa gatagctatg
     1081 gagccgggat gtctggtatt aaagccgtaa tcggacaaat agcgggcaag ggtcagggcg
     1141 gttataaatt tggcaaactc tgccggttga atactaaaag ggccaaacac cagccaggaa
     1201 cgggatcctt ttatctcggg tgcaatcagt agagtgagca acagcaaaaa cataacgata
     1261 atgtagccgt tgtaagacag gtaataataa aatttggcgt cgataagaag tataaagaaa
     1321 gccgtcacca gcgaaacagc tatccagatc aattgcttgc ccgcggtgct gttcaaatcg
     1381 aataggaccg cgctgtcgta attataactg gcgctaaaaa cggtgagcca gcctacgata
     1441 actaaaaaca gataaataac tatagtctgt atatctactg ctttccagag gcttatggga
     1501 ttaatctttg agttcaacgt tcataaggtt agcgttcaac aatcgggttt ccatccattt
     1561 tcgtccggga gcaatacttc ccttcaaata tttttccagc atcaggctgg ccataggcac
     1621 tgcccaggtg gctccgaaac ctccgttttc gataaagacc agcatacaga tctgcggatc
     1681 gtctttgggt gcaaaacata tgaatatgga gtggtccaca ccgggatttt cggccgtgcc
     1741 tgtcttgccg cagacctcta taccgggaat ggacactccc ttggaagttc ccatagggct
     1801 ggtgactgcg cctcgcattc cttcgatgat gggctcgaag tgttcgggat ctatcccgga
     1861 aacatgcttt tttattatgc ttgtgtctgc ttccatacca tgaacctgac gcacgatatg
     1921 cggagttata taccagcctt tgttgcatat cacggcgcaa taattggcca tttggagcgg
     1981 agtagccagg acttctccct ggcctatgcc attggatatg atcgtggccc ctctccagcc
     2041 tctttgaccg tatattccgt tataataatc cgtagtaggt atcaagcccg attgttcgta
     2101 gggcaggtct actcccagac ggctgccaaa gcctatggag cagatcaatc tgcgccagtg
     2161 ttcgtaagct tcctgcaccg aagaatattg ggggtggtcg atcatacttc gataggcggc
     2221 cgcaaagtaa gcattgcacg aagccgaaat cgcatcgggc agattcaggg gcgaaggatg
     2281 actgtggcag cccaacttct gtctggcaaa aaaataaccg ccgtaacagg caaattt
//
LOCUS       DAOSTD010000154         2333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_41824_length_2333_cov_19.115452, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000154 DAOSTD010000000
VERSION     DAOSTD010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41824_length_2333_cov_19.115452"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PLI37_07660"
     CDS             <1..623
                     /locus_tag="PLI37_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HOQ58921.1"
                     /translation="TVLGVGGGNLNDHMMEQIANKGNGNYEYIDNAKQIEKVFTHEIL
                     KFYTVAKDSKIQITFNPTMVDSYRLIGYENRKLNDEDFENDTTDAGEIGSSQTITALY
                     ELVLLDVPDTEKYAQFDFRYKKPNETQSRLIAHEINTTPKEMRFSSENMRFAASVAGL
                     GLLMKQSEYKGTLTKQMVLDLGREAKSFDPNGYRKEFIELVTDWNN"
     gene            919..>2333
                     /locus_tag="PLI37_07665"
     CDS             919..>2333
                     /locus_tag="PLI37_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOQ58922.1"
                     /translation="MKNYLKISLLLTPFLFLFSCTPKEKMETGRFEPTWESLSQYEVP
                     QWFRDAKFGIWAHWGPQCQPEAGDWYARNMYYQGHWQYRYHVEHYGHPSEVGFKDVIN
                     QWKAENWNPEELLALYKRAGAKYFFALGNHHDNLDLWNSKYQPWNSVAVGPQKDILAG
                     WAQAARNNGLPFGVSIHSAHAWRWYEGAQLSDKEGPLAGVPYDGKLSKKDGKGKWWQG
                     LDPQDLYAQNHELSEESENIGSIHHQWHWGNGALAPSEEYCLNFYNRTVDMINQIQPD
                     LIYFDDTGLPLWPVSNVGLEVAAHFYNSNMRLNEGKLEAVLFGKVLTEEQKACMVWDV
                     ERGAPDQIQELPWQSCTCIGGWHYERGIYNRNGYKSAKTVVQLLVDIVSKNGNMLLSI
                     PLRGDGSIDDKALKIVEDIAQWMDINSECIYETRPWHIYGEGPKAEQANPINAQGFNE
                     GRGGPYTADDIRFTRKGESLYA"
BASE COUNT          691 a          528 c          579 g          535 t
ORIGIN      
        1 tgaccgttct tggggtagga ggaggcaacc tcaacgacca tatgatggag caaatcgcca
       61 acaaaggcaa cggcaattac gaatacatcg ataatgccaa acaaatcgag aaagtgttca
      121 ctcatgaaat tttaaaattt tacaccgtag ccaaagattc aaagatacag atcaccttca
      181 atccaaccat ggtcgactcc tacaggttga tcggctacga aaaccggaaa ttaaacgatg
      241 aagactttga aaacgatacg accgatgccg gtgagatcgg ttcgtcgcag acaataacgg
      301 cattgtatga gcttgtattg ctcgatgtac ccgatacgga aaaatacgct caattcgatt
      361 tcaggtataa aaaaccgaac gaaactcaaa gcagacttat tgctcacgag atcaatacga
      421 ctccaaagga gatgagattt tcgtccgaaa atatgagatt tgccgcatcg gtggccggct
      481 taggcttgtt gatgaaacaa tccgaataca aaggaacgct caccaaacaa atggtattgg
      541 atttaggaag agaggccaaa tctttcgatc cgaacggcta cagaaaagaa ttcatagaat
      601 tggtgaccga ttggaataat taaaaaacct cgccaaatct tgcatacttc gtgcagccga
      661 ctgttgctca aaagaatgat tgcgcatctt cccgttgtta aaagcgacag tcggatcaac
      721 agctaccggt acgtgcgttg tattcgcaaa ttgttggccg gcgtcgaagc acaaagttca
      781 gattcagcta ccggcacctg tgtcgtccgt tccggtacag tcgaattatt tatgtaaatt
      841 agcggattga aaaaccggag agtatcgtcg aactcttaca tgaactaatt cgtaataacc
      901 agttaaacta tgaccaaaat gaaaaactac ttaaaaatca gcctgttgct aacccctttt
      961 ttgtttcttt tttcttgcac acccaaagaa aaaatggaga caggccggtt tgaacccact
     1021 tgggaatcgt tgagccagta cgaagtgccc caatggttca gagatgccaa atttggtatc
     1081 tgggcgcatt ggggacctca gtgtcagccg gaagccggcg actggtacgc caggaatatg
     1141 tattaccagg gtcactggca atacaggtat catgtggaac actacggaca tccttccgag
     1201 gtgggattca aagatgtgat caaccaatgg aaggccgaaa actggaaccc cgaggaattg
     1261 cttgctctat acaagcgcgc cggagctaag tacttttttg ccttgggcaa tcaccacgac
     1321 aacctcgatt tgtggaacag taagtaccag ccctggaatt ccgtggctgt aggtcctcaa
     1381 aaagacattt tggcgggctg ggcccaagcg gccagaaaca atggtctgcc tttcggcgtg
     1441 agcattcatt cggcccacgc ctggcgatgg tatgaaggag cccagttatc cgacaaggaa
     1501 gggccgctgg ccggcgttcc ctacgacggc aagctgagca aaaaagacgg caaggggaaa
     1561 tggtggcaag gcctggatcc tcaggacttg tatgcgcaaa accacgaact gagcgaagaa
     1621 tcggaaaaca taggcagcat ccaccatcaa tggcattggg gcaacggcgc tttggctcct
     1681 tcggaggaat attgcctgaa tttctacaac cgcacggtgg atatgatcaa tcagatccag
     1741 cccgacttaa tttatttcga cgacaccggt ttgcccttgt ggccggtcag caatgtagga
     1801 ttggaagtgg ccgcgcattt ttataattca aatatgcggc taaacgaggg caagctcgag
     1861 gccgttttgt ttggtaaagt gctcaccgag gaacaaaaag cctgtatggt atgggacgtc
     1921 gagcggggag ctcccgatca aattcaggaa ctgccctggc aatcttgtac ttgcatcgga
     1981 ggctggcatt acgaacgggg catctacaac agaaatggct ataaatcggc caaaactgtg
     2041 gtgcagcttt tagtggacat agtgagtaaa aacggaaata tgttgcttag tattcctctt
     2101 cgcggagacg gctccataga cgataaagcc ttgaaaattg tagaagatat cgctcaatgg
     2161 atggatatca acagcgagtg catttacgaa acccgtcctt ggcatatata cggcgaaggc
     2221 cccaaagctg aacaggccaa tcctattaat gctcaaggct ttaacgaggg taggggcggg
     2281 ccttacaccg cagacgatat tcgttttacc cgcaaagggg agtcgcttta tgc
//
LOCUS       DAOSTD010000155         2333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_41826_length_2333_cov_13.402546, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000155 DAOSTD010000000
VERSION     DAOSTD010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41826_length_2333_cov_13.402546"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLI37_07670"
     CDS             complement(<1..293)
                     /locus_tag="PLI37_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005848917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOQ58923.1"
                     /translation="MLIRLYEDNPNPRVVQKIANALREGGLIIYPTDTVYAIGCDIFH
                     ARAVEQICRLKDKCIGKANLSFICHDLSHISEFARLDNATFKLMKKNLPGPFT"
     gene            complement(315..>2333)
                     /gene="glgP"
                     /locus_tag="PLI37_07675"
     CDS             complement(315..>2333)
                     /gene="glgP"
                     /locus_tag="PLI37_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOQ58924.1"
                     /translation="LPIEQVKDEQGNPLVLKVDFPGYIVNCYIWKAAVGRVDLYLIDT
                     DHPGNSEFERQITAQLYGGDWENRLKQEYLLGIGGIQLLNILGIKKQVYHCNEGHAAL
                     INAQRMVDLIAQEGLNFSEALEVVRASSLYTVHTPVPAGHDKFDEDLMFKYMSSFPQK
                     LGISWARFMDMGRENEGDTNEKFSMSVFACNCCQEVNGVSMLHGKVSQKMFSNIWKGY
                     FPDELHVSYVTNGVHMPTWTASEWRSLYEKTFGPQWMGDQSNAELWSKIYEVSDEEIW
                     KTRQQLKLKLIEYIRNKYRDSWFKNQGDPSRIMAILEKINPNTLMIGFGRRFATYKRA
                     HLLFTDLERLSRIVNNPLYPVQFVFTGKAHPADGGGQGLIKRIVEISRMPEFQGKIIF
                     LENYDMALAKRLISGVDIWLNTPTRPLEASGTSGQKAEMNGVLNFSVLDGWWLEGYVE
                     KAGWALTEKRTYESQEFQDQLDAATIYSMLENEIIPLFFAKNSKGYSPEWVQYIKNSI
                     AQIAPRFTTKRMLDDYIDRFYSKLVKRSALITANSYAKAREISRWKQEVADKWEQIKV
                     LKVDFPDAMLHQPKVGSEYNIGVSIDSAGLKDSIGVELVILHTNLDGSSQNYSSELEL
                     KNIDEKGHLRFEINYRLKKAGAFDYSFRMFPKHPDMPHRMDFCYVRWF"
BASE COUNT          546 a          549 c          592 g          646 t
ORIGIN      
        1 aaagtaaagg ggccgggcag attctttttc atcagtttaa aggtggcgtt atccagcctg
       61 gcaaattcac tgatatggct cagatcgtgg caaataaacg acaaattggc tttgcctatg
      121 catttgtctt tgagccggca aatctgctcg acagccctgg catgaaaaat gtcgcaaccg
      181 atggcataga ccgtatcggt gggataaatg attaatcccc cttcgcgcaa tgcattggct
      241 attttttgca cgacccttgg gttgggattg tcttcataaa gccgaatcaa cattgttcac
      301 aaatttgacg ctaattaaaa ccagcggaca taacaaaagt ccatacgatg cggcatatcg
      361 gggtgcttgg gaaacattct gaaagaataa tcaaaagcac cggccttttt caaacgatag
      421 ttgatttcaa aacgcagatg gcctttttcg tctatatttt tcagttccag ttcagatgaa
      481 taattttgag aactgccgtc gaggttggtg tgcaaaatta ccaattccac accgatgctg
      541 tctttgagac cggccgagtc gatgctcaca ccaatgttgt attcgctgcc cactttgggc
      601 tgatgcaaca tggcatcggg aaaatctacc ttcagcacct tgatctgctc ccatttatcg
      661 gctacttcct gtttccagcg gctgatttca cgggccttgg cataggaatt ggcggtgatc
      721 aacgccgaac gtttaaccaa tttgctgtag aagcgatcga tgtaatcgtc gagcatacgc
      781 ttggtggtga aacgcggggc gatttgagca atggaattct tgatgtattg cacccactcg
      841 ggcgaataac ccttactgtt ttttgcaaag aacaagggaa taatttcgtt ttcaagcatg
      901 gaataaatgg tggctgcatc gagctgatcc tggaactcct ggctttcgta ggtacgtttt
      961 tcggtcagag cccagccggc tttttcaaca taaccttcga gccaccagcc gtcgagcacc
     1021 gagaaattga gcacgccgtt catttcggct ttttggcccg aggttcccga agcttctaag
     1081 ggacgggtcg gggtgttgag ccaaatgtcc acacccgaaa tcaggcgttt ggccaaggcc
     1141 atgtcgtagt tctcgaggaa gatgatcttg ccctggaatt cgggcatgcg cgaaatttcc
     1201 acgatgcgct taatcagtcc ctgtccgcct ccgtcggccg ggtgggcctt gccggtaaag
     1261 acaaattgca cgggataaag gggattgttc actatgcggg acaaacgttc cagatcggta
     1321 aacaacaaat gggcacgttt gtaggtagca aaacgacgac cgaaacctat catcagggta
     1381 ttgggattaa ttttttccaa tatggccatg atgcgcgagg gatcgccctg gtttttgaac
     1441 caactgtctc tgtatttgtt gcgtatgtac tcaatcaatt taagcttgag ttgctgacgg
     1501 gttttccaaa tctcttcgtc gctgacttcg tagattttgc tccagagttc ggcgttggac
     1561 tgatcgccca tccattgggg accgaaagtt ttttcgtaga ggctgcgcca ttcgctggcg
     1621 gtccaggtgg gcatatgaac gccgttggta acataactta catgtaactc gtcggggaaa
     1681 taacctttcc agatattgct gaacattttt tgggacacct tgccgtggag catgctcacg
     1741 ccattgacct cctgacagca gttgcaggca aaaacgctca tgctgaattt ttcgttggtg
     1801 tcgccttcgt tttcgcggcc catatccata aatctggccc aactgatgcc caatttttgg
     1861 gggaagctgc tcatgtattt gaacatgagg tcttcgtcga atttatcgtg gccggcggga
     1921 accggggtat gtaccgtgta gagcgaagag gcgcgtacta cttcgagggc ttcgctaaag
     1981 ttaagtcctt cctgggcaat caggtcgacc atacgttggg cgttgatcag ggcggcgtgg
     2041 ccttcgttgc aatgatatac ctgttttttg atacccaaga tattgagcag ttgtatgccg
     2101 ccaatgccca gcaggtattc ctgtttcaag cggttttccc agtcgccgcc gtagagttgg
     2161 gcggtaattt gacgctcaaa ctcgctgttg cccggatggt cggtatctat caggtagaga
     2221 tccactctgc ccacggctgc tttccagata tagcaattca ctatatagcc cgggaagtcc
     2281 actttaagca ccaggggatt gccttgttcg tctttgactt gttctatggg cag
//
LOCUS       DAOSTD010000156         2267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_43468_length_2267_cov_14.523508, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000156 DAOSTD010000000
VERSION     DAOSTD010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43468_length_2267_cov_14.523508"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLI37_07680"
     CDS             complement(<1..389)
                     /locus_tag="PLI37_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOQ58925.1"
                     /translation="MLKKIVAVSGKPGLYKMLSQGKNMLIVESLVDGKRMPAYNKDKV
                     MSLGEISMYTENGEKPLHEVLELLKTKAGGEKLSIDPKASNDELRAYFADVLPDYDRE
                     RVYPSDIRKLLNWYNLLIEKGYTNFAPV"
     gene            complement(458..1042)
                     /gene="coaE"
                     /locus_tag="PLI37_07685"
     CDS             complement(458..1042)
                     /gene="coaE"
                     /locus_tag="PLI37_07685"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOQ58926.1"
                     /translation="MKILALTGGIGSGKSTVAEIFRILGIPVFDADSAAKELYETNPA
                     LRKGLIALFGRGIYDENNKLNTKLLASTAFNDPVRLAQLNKLVHPPVIEYFCEWKQKH
                     GNYPLLVHETALLYEAGLDKLSDYVAVVTAAENLRIERVCRNKGWSQEQVRERIRRQL
                     SQDLLKSKADFIIDNNGQLALLPQVIELIKKVQN"
     gene            complement(1050..1976)
                     /locus_tag="PLI37_07690"
     CDS             complement(1050..1976)
                     /locus_tag="PLI37_07690"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HOQ58927.1"
                     /translation="MNKEFYVFLLFLGLSILFWFLQSTQATSQTELSIPLRYGKLPEN
                     ITVTNELPDCIKVTVRDKGSHLYNYHIHRRKLGLELDLLRWRADDGIGRISLEQFKPV
                     IFAKLKPSAQILRISPDSLILYFVEKEQKDLPVKLNAQVALSRQYMLVEEPSIQPAFV
                     RVYAPSSILDSLELVETEFLDLPVLRDSVSMDVKLKAIEGVHFDQTSVRVHFPVEEFT
                     EVRYEIPVTGLDFPENLLLRSFPSKVVVSFLVSKSNYQSVKAEDFQLGISYRDLIESN
                     EDLQSVKLIKTPDYVQRLSLHPDKVECLIEKK"
     gene            complement(2048..>2267)
                     /gene="yajC"
                     /locus_tag="PLI37_07695"
     CDS             complement(2048..>2267)
                     /gene="yajC"
                     /locus_tag="PLI37_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOQ58928.1"
                     /translation="MIRPQNKKQKEIQKARAALQVGDRVVTSGGIYGKLRDIKDDAFI
                     IEISENVRIRIEKTAVFAAGEPAANSSK"
BASE COUNT          599 a          496 c          496 g          676 t
ORIGIN      
        1 accggagcaa aatttgtgta gcctttttca atcagtaaat tgtaccaatt cagcaatttc
       61 cggatatccg aagggtaaac cctttcccgg tcgtaatcgg gcagcacatc ggcaaaatag
      121 gctctgagtt cgtcgttgct tgctttggga tcgatactta atttttctcc tccggcctta
      181 gttttaagca attccagtac ttcgtgcaat ggtttttcac cgttttcggt atacatcgaa
      241 atctcgccca gggacatcac tttatctttg ttgtaagcgg gcattctttt tccgtcgacc
      301 aaggactcta ctatcagcat atttttgccc tggctgagca ttttatacaa acctggtttg
      361 ccggagacgg ctacaatttt tttcaacatc ttggtaatta attttttagg tgagttattg
      421 cattagttta aaaatctgct tttaggaaag atgtccttca gttctgtact tttttaatca
      481 gttctataac ctggggcaac aaggcaagct gaccgttgtt atcgatgatg aaatcggcct
      541 tggatttcag caaatcctgg gacaactgcc ggcggatacg ttccctaacc tgttcctgac
      601 tccagccttt gttgcggcag actcgttcaa tgcgcaagtt ctcggctgca gtaaccactg
      661 caacgtagtc gctgagtttg tccaaccctg cctcgtacaa aagggcggtt tcatgcacaa
      721 gcagggggta attcccgtgt ttttgtttcc actcacagaa atattcaatc acgggcggat
      781 ggaccaattt atttaactga gcaagtcgca cagggtcatt aaaagcggtg gaagccagta
      841 atttggtatt aagtttattg ttttcgtcgt atattcccct gccaaataag gcaatgagcc
      901 ccttgcgtaa ggcagggttt gtttcgtaca gctctttggc cgcagaatct gcatcgaaca
      961 caggaatgcc cagtattctg aaaatttctg caacagtcga tttgccgctg ccaataccgc
     1021 cggtcagagc caaaattttc atcggaaaat tatttttttt cgatcagaca ttcaacctta
     1081 tccgggtgca gactgagtcg ctgtacataa tccggggtct ttatcaattt gacgctctgc
     1141 aggtcttcgt tcgattcgat taaatcgcgg taagatatgc ctaattggaa atcttccgct
     1201 ttgaccgatt gataattgga cttgcttacc aaaaaagaaa caaccacttt cgaagggaaa
     1261 gagcgtaaaa gcagattttc gggaaaatcc agccccgtaa cggggatttc gtaacgaact
     1321 tcggtaaatt cctcaaccgg aaaatgtact cgtacgctag tctgatcaaa atgaacacct
     1381 tctatggctt tgagttttac atccatgctc acagaatctc tcagtacagg tagatccagg
     1441 aattcggtct ctaccagctc aagactatcc aggatggatg aaggagcgta tactctgacg
     1501 aaagcgggtt gtatggaagg ttcctcaacc agcatatatt gccgtgaaag agctacttga
     1561 gcatttagtt tgacaggcag gtctttttgt tctttttcga cgaaatacaa aatcagactg
     1621 tccggactga tcctcaaaat ttgagcactg ggcttaagtt ttgcaaaaat gaccggtttg
     1681 aattgctcaa gtgaaattct gccgatacca tcgtcagcac gccaacgtaa caaatcaagc
     1741 tcaaggccga gttttcgccg gtggatatga taattgtaaa gatgactgcc cttgtcccgt
     1801 acggttactt ttatacagtc gggtaattcg ttggttacag ttatgttttc gggcagtttc
     1861 ccgtatcgca agggaatgga caattctgtt tgcgaagtgg cctgggtgct ttgaagaaac
     1921 caaaataaaa tggaaaggcc caaaaacaac agaaaaacat aaaactcctt gttcatattc
     1981 aaaaacagga gacgatatgt ttttctgaag aagtattgga cccgggtcca ttttaattta
     2041 aaacccatta tttcgaagaa ttggcagccg gttcgcctgc agcgaatacc gctgtttttt
     2101 cgatacgtat acggacgttc tccgagattt caattataaa agcgtcgtct ttaatgtcgc
     2161 gaagtttacc gtaaataccg ccggaagtta caacgcggtc accaacctgc agggcggcac
     2221 gggctttttg aatttctttt tgtttcttgt tttgcgggcg gatcata
//
LOCUS       DAOSTD010000157         2265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_43510_length_2265_cov_16.417195, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000157 DAOSTD010000000
VERSION     DAOSTD010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43510_length_2265_cov_16.417195"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="PLI37_07700"
     CDS             complement(<1..1390)
                     /locus_tag="PLI37_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOQ58929.1"
                     /translation="MEVTRLFDLLDNYLEKYPDQAVALAGKVNHQWVRYSIQDYVKEV
                     NRLSYAFLAKGVKPGCKIGIISNNCPEWNFFDMAIMQIGAVGVPIYPTISQSDYKYIL
                     KHSDVEYVFVDSKELVSKLQPLVADNPLFKEFICLQETENAVSLADFRALGDANPNPK
                     EVTKRKAGIKEDDMASIIYTSGSTGMPKGVMISHKNILCQIMDFYHVPAEWSKTALSF
                     LPLCHAYERLLIYLYHFLGISIYYAENIGTIAENIKEVKPTMMTAVPRVLEKIYDKLF
                     LAGKSQKGIKKNLYYWAFELATRYKLEGNGWWYDLQHTIADKLIYSKWRQAIGGNFDL
                     IVSGGAAIQKHQSAFFNAIKIPVFEGYGLSETAPVIAVSRRGKNNRREGTVGPPLTCV
                     EVKITEENEIICRGPNVMLGYYKAPELTAEVIDKDGWFHTGDTGKFTPEGLLIITGRL
                     KHIFKTSFGKYIN"
     gene            complement(1511..1990)
                     /locus_tag="PLI37_07705"
     CDS             complement(1511..1990)
                     /locus_tag="PLI37_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOQ58930.1"
                     /translation="MSIEIERKFLVKASFKEFSKSNVYIVQAYLSIGPKCSIRVRICG
                     DKAYLTIKGETSASGLSRYEWERVLTVDEARDLIKLSEFEAIEKTRYFIPQGKHCYEV
                     DVFEGKNSGLIVAELELESEDETYEKPSWLGAEVTGDIRYYNANLQINSYSEWKDDV"
     gene            2060..>2265
                     /locus_tag="PLI37_07710"
     CDS             2060..>2265
                     /locus_tag="PLI37_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58931.1"
                     /translation="MKTKISWLILPFMVWGLSFSLAAAPATPKAFVRQFEKFVEEVEK
                     SHQEYKLEDWKLAEEKYKEFVKTH"
BASE COUNT          622 a          478 c          466 g          699 t
ORIGIN      
        1 ggttgatgta tttgccgaag gaggtcttaa agatgtgttt caatcgaccg gtaatgatta
       61 ataaaccctc gggagtaaat tttcccgtgt cgcctgtgtg gaaccagccg tctttatcta
      121 tgacttctgc agtcaactca ggcgctttgt agtagccgag cattacgttg gggccgcggc
      181 aaatgatttc gttttcttcg gtgattttta cctccacaca agtgagagga ggcccaacag
      241 tgccttctct acggttgttt ttgcctctgc ggctcacggc aattacggga gctgtttcgc
      301 tgagtccgta accttcgaac accggtattt taatggcatt aaaaaaggca gattggtgtt
      361 tttggatagc tgcacctcct gaaacaatta aatcgaaatt ccctccgata gcctggcgcc
      421 atttagaata gataagttta tcggctattg tatgttgcag atcataccac cagccattcc
      481 cttcgagttt gtagcgtgtg gccagttcaa aggcccagta gtaaagattt ttctttatac
      541 ccttttgaga cttacctgcc aggaacagtt tgtcgtatat tttttccaat actcgcggaa
      601 cagccgtcat catggtgggt ttaacttctt taatgttttc ggctatggtg ccgatattct
      661 cggcataata aatagagatt ccgagaaaat ggtacaaata aatcaatagg cgttcataag
      721 catgacaaag aggtaaaaag ctgagggctg ttttactcca ttcggcgggg acatggtaaa
      781 aatccatgat ttggcaaagg atattcttat gactgatcat aactcctttg ggcatgccgg
      841 tgctgccgga agtatagata atgctggcca tgtcgtcttc tttgatgccg gcttttcgct
      901 tagtcacctc tttgggattg ggattggcat cacctaaggc acgaaaatcg gccaggctta
      961 cagcattttc agtttcttgc agacatataa actctttaaa taaagggttg tctgccacta
     1021 aaggttgaag tttgcttacc aattctttac tatccacaaa gacatattct acatcggaat
     1081 gctttaatat atacttgtaa tcgctttgac taatggtggg ataaatgggt acaccgacag
     1141 ctccaatctg catgatggcc atatcaaaga agttccattc cggacagttg ttactgataa
     1201 taccgatttt acagccgggc ttcactcctt tagccagaaa agcataactc aagcgattaa
     1261 cttctttaac ataatcctga atactgtaac gaacccattg gtggtttact ttgccggcca
     1321 aggctactgc ctgatcgggg tatttttcca gataattgtc gagcagatca aatagacgag
     1381 taacttccat acatttcctc tttttacaat aaacttattt tgaaatattc aaaccgcaaa
     1441 aatagacata aaaatcgtgt cagaataaaa aagtggtcaa aaactgaata gagggacgat
     1501 tgcccccgga ttaaacatca tctttccatt ccgaataaga attgatttgc agattcgcgt
     1561 tataataccg aatgtctcct gtgacttcgg ctcccagcca ggagggtttt tcataagttt
     1621 cgtcctcgct ctccagttcc agttccgcca caatcaatcc gctgttttta ccttcaaaca
     1681 catctacttc gtaacaatgt tttccctgag gaataaagta acgcgttttt tcaatagctt
     1741 caaattcaga cagtttgatt aaatcgcggg cttcgtccac tgtcagcacg cgttcccatt
     1801 cgtaacggct caggcccgat gcggaagttt cgcctttgat ggttaaatag gctttatcgc
     1861 cgcaaatcct gacgcgtata ctgcatttcg gtccgatact caagtaagcc tgtacgatat
     1921 aaacattgct tttggaaaat tctttaaaag aggcttttac taaaaatttt cgctcgattt
     1981 cgatggacat aaatcttgtt tttgccgtta aaattagaaa ttttacttta ccttcgttct
     2041 gtttttcaga cggattatta tgaaaacaaa aatcagttgg cttattttgc cgtttatggt
     2101 ctggggctta agcttttccc tggctgctgc tcctgccacg cctaaagctt ttgtaagaca
     2161 gtttgaaaag tttgtcgaag aagtggaaaa atcacatcag gaatacaaac tggaagattg
     2221 gaaattggcc gaagaaaaat acaaggagtt tgttaaaacc catta
//
LOCUS       DAOSTD010000158         2225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_44605_length_2225_cov_11.741014, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000158 DAOSTD010000000
VERSION     DAOSTD010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44605_length_2225_cov_11.741014"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLI37_07715"
     CDS             <1..354
                     /locus_tag="PLI37_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA synthetase"
                     /protein_id="HOQ58932.1"
                     /translation="MDDVIKSSGYRIGPFEVESAVMTHPSVVECAITGIPDEIRGQVV
                     KASIILAKEYRDHDKEALAKEIQEHVKQVTAPYKYPRVIEFVNELPKTISGKIRRVEI
                     RQDDSEKINVKKRQP"
     gene            351..1220
                     /locus_tag="PLI37_07720"
     CDS             351..1220
                     /locus_tag="PLI37_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOQ58933.1"
                     /translation="MTLSMTGYGKAQADIGKRKISIELRSLNSKQFDLSMRIPSLLKE
                     KETEIRNEIQKRLERGKIDLLIQVETEQTDTPCSINRSLLETYYRDILETAGTLGINP
                     PSDPWPVLLKLPDVLKGEHPDMEAEEWTEIMELLNRAIDQLNDFRKQEGEMLKAFFQN
                     KLQAIESLLGELETYESERVDKIKSRLEEGLKKVAVQDYDRNRFEQELIYYIEKLDVS
                     EEKSRLINHCEYFKATLNAGSKGKKLGFIVQEMGREINTLGSKANHAEMQRLVVCMKD
                     ELEQMKEQVLNVL"
     gene            1217..1729
                     /gene="nadD"
                     /locus_tag="PLI37_07725"
     CDS             1217..1729
                     /gene="nadD"
                     /locus_tag="PLI37_07725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOQ58934.1"
                     /translation="MKRIGIFGGTFDPIHYGHISVAEHALRQAGLDELWFVVSPHNPL
                     KKEEEIKEAGQRLQEARKALTEFPEFKVSDIEFNLPRPSYMAQTLRTLQKKYPGNDFV
                     LIIGGDNLEVFEQWKDYRFLLDNFDILVYPRPGSPNRVPGEWTRVSLLDAPLMDVSST
                     RIRKTQQGRK"
     gene            1757..>2225
                     /locus_tag="PLI37_07730"
     CDS             1757..>2225
                     /locus_tag="PLI37_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOQ58935.1"
                     /translation="MSFKKRFSRFLFKCAGWKAVALTPDYNKCVLVVAPHTSNWDFVV
                     GKLAYNALGREGNFLIKKEWFRFPFNLFFKAIGGIPVDRGKRQRMTEAIIDLFSERDH
                     FCLGITPEGTRKPNARWKRGFYHIARSAKVPIVLVKLDYGNKCVTLFRVFHPSG"
BASE COUNT          677 a          533 c          520 g          495 t
ORIGIN      
        1 atggacgacg ttatcaaaag ctcaggttac cgtatcgggc cgttcgaagt ggaaagcgcc
       61 gttatgaccc atccttcggt ggtggaatgt gccattaccg gcatacccga cgaaatcaga
      121 gggcaagtgg tcaaagccag catcattctg gccaaagaat accgcgatca cgacaaagaa
      181 gccctggcca aagagataca ggaacatgtg aagcaggtga ccgctcccta caaatatccg
      241 cgtgtaattg aatttgtaaa cgaattaccc aaaaccatca gcggcaagat cagacgagtc
      301 gaaatccgcc aggacgacag cgaaaaaata aacgtcaaaa aacgacagcc atgacattat
      361 ccatgacagg ttacggtaag gctcaggctg atatcggtaa gagaaaaatc agtatcgagt
      421 tgcgttcgct caacagcaaa caatttgacc tttctatgcg catcccttct ctgttgaaag
      481 aaaaggaaac cgaaatccgt aatgaaattc aaaaacgcct ggaacggggc aaaatcgatc
      541 tgctgataca agtggaaacg gagcagaccg atactccctg cagcattaac cgcagtcttt
      601 tggaaactta ttatcgcgat attctcgaaa cggccggtac tctgggtatc aatcctccct
      661 ccgacccctg gccggttctg ctcaaattgc ccgatgtttt gaaaggcgaa catcccgata
      721 tggaagctga ggaatggact gagatcatgg aactgctcaa ccgggccatc gatcaattga
      781 atgacttcag aaaacaggaa ggtgaaatgc tgaaagcctt tttccaaaat aaactgcaag
      841 ccatagagtc tctgctcggg gaactggaga cctacgaatc cgaacgtgtt gataaaataa
      901 aatcccgcct ggaagaaggc ctaaagaaag tagccgtcca ggattacgat cgtaaccgtt
      961 ttgagcaaga gttgatttat tacatcgaaa aactggacgt aagcgaagaa aaatcccgcc
     1021 tgatcaacca ctgcgaatat ttcaaagcca ctctcaatgc cgggagtaaa ggcaagaaac
     1081 tgggttttat cgtacaggaa atggggcgtg aaatcaacac cttgggctct aaagccaatc
     1141 acgccgaaat gcaacgcctg gtcgtgtgca tgaaagacga actggaacaa atgaaagagc
     1201 aggtattgaa cgtattatga aaagaatcgg catttttggc ggtaccttcg accctattca
     1261 ttacggacat atttctgtgg ctgaacacgc cctgagacaa gccggactcg atgaattatg
     1321 gtttgtagtc agtccgcaca atccactcaa aaaggaagag gaaatcaaag aggccggaca
     1381 acgcttgcaa gaagctcgca aggcattgac cgaatttccg gaattcaaag tttccgacat
     1441 agaatttaat ctgccccgtc cctcttatat ggcgcaaacc ctgcgaacgc tacaaaaaaa
     1501 ataccccggg aacgactttg tcttgatcat cggaggcgac aacctggagg tatttgaaca
     1561 atggaaagac taccggtttt tgctcgataa tttcgacatt ctcgtttatc cgcgtccggg
     1621 cagtcccaac cgggtgcccg gtgaatggac cagagtgagt ctgctcgacg ctcccctgat
     1681 ggatgtgtcc tctacccgga ttcgcaaaac acaacaagga agaaaataaa caccagttct
     1741 taaacactca gactctatgt catttaaaaa acgtttctca cgcttcttgt tcaaatgtgc
     1801 cggatggaag gccgttgccc ttacaccgga ctacaataaa tgtgtgttgg tagttgcccc
     1861 ccacaccagc aattgggatt tcgttgttgg gaaactggcc tacaatgcct tgggcagaga
     1921 aggaaatttc ctgataaaaa aagaatggtt tcgctttccc ttcaaccttt ttttcaaagc
     1981 catcggaggc atacccgtag acaggggaaa aagacaacgt atgaccgaag ctattataga
     2041 cttgttttcc gaacgtgatc atttttgttt gggcattacc ccggaaggca cccgtaagcc
     2101 caacgccaga tggaagaggg gcttttacca catcgccagg agcgccaaag tgcccatcgt
     2161 cttggtaaag ctcgattacg gcaacaaatg cgtcactctg ttccgggtat ttcatcccag
     2221 cggcg
//
LOCUS       DAOSTD010000159         2199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_45294_length_2199_cov_18.816698, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000159 DAOSTD010000000
VERSION     DAOSTD010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45294_length_2199_cov_18.816698"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..>2199)
                     /locus_tag="PLI37_07735"
     CDS             complement(93..>2199)
                     /locus_tag="PLI37_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOQ58936.1"
                     /translation="FSAEGKSLLSEKEAGASFSEFNDAGTKTFSVKQSFVLDQDETIY
                     GLGQQQDARMSKRNVRLNMVQGNTDDYVPFLLSIKAYGLFWDNYSPTVFEDNAAGCYF
                     DSQVGECIDYYFMYGGHADGVIARMRELTGQVPMFPLWTYGFWQSRERYKSQEELLTV
                     VRKYRELEVPLDGIIQDWQYWGNNYLWNAMDFLNPNFPNPQGMVDEVHQLNAHIIISI
                     WNSFGPQTKQYKELEKMNALFDMTTWPPYGPERHPVNQEYPSGVKVYDVYNPKARELY
                     WRYLDKGLFSLGMDGFWIDSSEPDHLNFKPSDFDNQTYLGSFRKVRNAFPLMAVGGVA
                     EQQRAKSSDKRVFILTRSAFAGQQRYGANLWSGDVSASWQALHAQITAGLNVSLSGIP
                     HWNSDIGGFFLWRFRNPLNNPDYRELHVRWMQFGCFCPMMRSHGEGSPREIYQFGQKG
                     NPIYDALEKYIQLRYRLLPYIYSTSYEVSALQSSMMRALMMDFPQDPAVLDLNSEYMF
                     GKALLVCPVTEPMYTTYVPRGRDSVLREDFSKVKTKEVYLPAGTAWYDFWTGEKMDGG
                     RKVGKEVPLDIMPLYVRAGSILPWGPKVQYATEQNWDQLEIRVYPGADGEFVLYEDEF
                     DNYNYENGAYSTISFKWDEKQKALTIGARQGNFPGMLLNRSFRIVFVEGSKGSGPDSS
                     EHYDQVVKYSGKKVVVRKR"
BASE COUNT          559 a          555 c          519 g          566 t
ORIGIN      
        1 cttcatcgac aggctgataa cttcccaggt aaaagacatt ccatttatta aaattgaatg
       61 atctagtatc catcgatgat atcaacgaac ctctatcttt ttctgacaac tacttttttg
      121 ccgctgtact ttaccacctg atcgtagtgt tcgctcgagt cggggccgct gcctttgctg
      181 ccttcaacaa aaacgatgcg aaaacttcgg tttaaaagca tgccggggaa atttccctgc
      241 ctggctccga tggtcagggc tttttgtttt tcgtcccatt taaagctgat ggtggaataa
      301 gccccgtttt cgtaattgta attatcgaat tcgtcttcgt acaacacaaa ttcaccgtct
      361 gccccgggat aaacacgaat ctcgagctga tcccagtttt gttcggttgc gtattgcact
      421 ttggggcccc agggcaaaat ggaacctgcc cgcacgtaga gaggcatgat gtctaaagga
      481 acttctttgc cgaccttacg gcctccatcc attttttcgc cggtccaaaa gtcgtaccaa
      541 gccgtacccg ccggcagata cacttctttg gtttttactt tgctgaaatc ttctctcagc
      601 accgaatccc ggcctctggg tacataggtt gtgtacatag gctcggtgac gggacaaacc
      661 aaaagggcct tgccaaacat atattcgctg ttcaaatcca gcacggcggg atcctgcggg
      721 aaatccatca tcagggcgcg catcatgctc gactgcaggg cactcacttc gtaggaagtg
      781 gaataaatat aaggcagcag gcggtaacgc aactgaatgt atttttccaa ggcatcgtag
      841 atgggatttc ctttttgacc aaattgatag atctcgcggg gagagccttc tccatgagaa
      901 cgcatcatgg ggcaaaaaca gccaaactgc atccagcgca catgcaattc ccgataatcg
      961 ggattgttta agggattcct gaatctccac aggaaaaacc cgccgatatc gctgttccag
     1021 tgggggattc ccgacaatga gacattcagg cccgccgtga tttgagcgtg taaagcctgc
     1081 caggaggccg atacgtcgcc cgaccaaaga ttggctccgt agcgctgctg gcctgcaaaa
     1141 gcagaacggg tcagtataaa tacgcgcttg tcggacgact tagccctttg ctgttcggcc
     1201 acaccaccca ctgccatcag aggaaaagcg ttgcgtactt tgcggaaaga acccaggtag
     1261 gtttggttat cgaaatccga aggcttgaaa ttcagatgat cgggctcgga agaatcgatc
     1321 caaaagccgt ccatacccag agaaaacagc cctttgtcca aatacctcca atacaattct
     1381 cttgccttgg gattgtagac gtcgtacacc tttactcccg aaggatattc ctgattgacc
     1441 ggatgtcttt cgggaccgta aggaggccag gtggtcatat caaaaagggc attcattttt
     1501 tcgagttctt tgtattgttt ggtctgcgga ccaaaggaat tccagatgga aataataata
     1561 tgagcattga gttgatgcac ttcgtcaacc attccttgcg ggttggggaa gttggggttc
     1621 aaaaaatcca tggcgttcca caaataattg ttgccccagt actgccaatc ctgaataata
     1681 ccgtccaggg gcacttcgag ttcccggtat ttacgcacta ccgtcagcaa ttcttcctga
     1741 cttttgtagc gttcgcggct ttgccaaaaa ccgtaagtcc acaaagggaa cataggaact
     1801 tgcccggtga gttcgcgcat acgggcgatt actccgtcgg catgaccgcc gtacataaaa
     1861 taataatcga tgcactctcc tacctgcgaa tcgaaatagc agccagcggc attgtcttcg
     1921 aacaccgtgg gggaatagtt atcccaaaac aagccatagg ctttgatgga cagcaagaaa
     1981 ggaacatagt cgtcggtatt gccctgcacc atattcaatc tgacgttgcg tttggacatc
     2041 ctggcgtcct gttgctgccc cagaccgtaa atagtctcgt cctggtcgag gacaaaagac
     2101 tgttttaccg aaaaggtctt agtgccggcg tcgttgaatt cgctaaagga ggctccggct
     2161 tctttttcgc tcagcaaaga ttttccttcg gcgctaaaa
//
LOCUS       DAOSTD010000160         2193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_45496_length_2193_cov_11.627689, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000160 DAOSTD010000000
VERSION     DAOSTD010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45496_length_2193_cov_11.627689"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PLI37_07740"
     CDS             <1..767
                     /locus_tag="PLI37_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58937.1"
                     /translation="SPVALSNGKAYYLNHLKIPNHVVIYRVKAKFYELWGAMTDEQKE
                     QYFRWTNWVEPLIVNDAQSSFTLTETMPTMYYHVLTAIPSEEAIADSLVCSVNYDGLL
                     YNSKTRKLTEVHLPAGEYYLRMGFKHSLRYCISIYFNDELLVEDMLLFAQGSNFHFDR
                     GSVSDMDYFGSSTIGFPEGYNWRDWIEKNEKAVAYDTDGYQVAIVNLPHSGNFTITIE
                     SNDNSYLYTTVNGRSKNNVTQLMMYHWCLRPTTNNY"
     gene            779..>2193
                     /locus_tag="PLI37_07745"
     CDS             779..>2193
                     /locus_tag="PLI37_07745"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOQ58938.1"
                     /translation="MIKYARYSVVLFMLFTGFSAVAAKLDGKVLSIDKSPVEGAIISV
                     VLEGRDFSAITAEDGSFSLELPSGRGYIRVVAAGFYRQEYPINQKLNLEEIVLVPLVS
                     PHYSGAAYFPNYNLSRDDKSTSTQSVEKKDFSKHFSIDQAIQDKVPGLYIHRKSGMPG
                     EGAYLNLRGIHSVVADNTPLIVINGIPLLGNQELSATVRAYSRNTLFGYSTDDIRSIT
                     LLKGADASVYGSLGSNGVLLIETEQATSDNLETRISFSGQYGLSWPGRSLPVLNVSQY
                     KNYLREIGMTRYDMITALYTDYPFLQNEENYYSYLFNNTTDWRGLVQNRAFITDNTFR
                     VEGGDEIAKYNISFGYTSEGGVLGQTASDRYHTLINSNIMVSRNVDIFTNVGLAYISS
                     SLQEQGMNAPTNAVLSSYFAMPLLSPYMKEPNNNVLDRYATYNGWNVNNNPTYPYDNV
                     SNPLAIVNTINASDNIYDANI"
BASE COUNT          591 a          515 c          519 g          568 t
ORIGIN      
        1 gtagtccggt agctttgagc aatggaaagg cttattacct gaaccatctg aaaataccca
       61 atcatgtggt gatttacaga gtcaaggcga aattttacga actttgggga gccatgaccg
      121 atgagcaaaa ggagcagtat tttcgctgga ccaattgggt ggagcctttg atagtgaacg
      181 acgctcaatc ttcctttacc ctgaccgaga ccatgcccac catgtattat catgtactca
      241 cggccattcc ttcggaagaa gccattgccg attctctggt ttgcagcgtt aattacgacg
      301 gtttgttgta caattccaag accagaaaac tgaccgaagt tcatttgccg gccggtgaat
      361 attacctgag gatgggattc aaacacagtc tgaggtattg catcagcatt tatttcaacg
      421 atgagttgtt ggtcgaagac atgttgctct ttgcccaggg atccaatttt cacttcgacc
      481 gcggttcggt gagcgatatg gactatttcg gcagttctac cataggcttt cccgaaggct
      541 ataattggag agactggata gaaaaaaacg aaaaggccgt ggcttacgat accgacggtt
      601 accaggtggc catcgttaat ctgccccata gcggcaattt taccattacc atagagtcga
      661 acgacaattc ttatttgtat acgacagtta acggacgcag taaaaacaac gtcacccagc
      721 ttatgatgta tcactggtgt ctgaggccaa ccaccaataa ctattaaaaa gggataatat
      781 gataaagtac gccagatatt cagttgtttt attcatgttg ttcacgggtt tttcggcagt
      841 agccgccaag ctcgatggca aggtgctgag tatagataag tctcccgtgg agggggccat
      901 tatctcggtt gttttggaag gacgggattt ttccgctata acggctgagg atggaagttt
      961 tagtctggaa ttgccttcgg gcaggggtta tataagggta gtggcggccg gtttttaccg
     1021 gcaggaatat cccattaatc aaaagctgaa tcttgaagaa attgtgcttg ttcctcttgt
     1081 cagtccccat tacagcggtg cggcctattt cccgaattac aacttgagtc gcgacgacaa
     1141 aagcaccagt acccagtcgg tcgaaaagaa agatttcagt aagcatttct ccatcgatca
     1201 ggccatacaa gacaaagtgc ccggtttata tatccatcgt aaaagcggta tgcccggcga
     1261 aggagcctac ctcaacctga gaggtattca ctctgtggta gccgacaata cgcctttgat
     1321 agtgatcaac ggtattcccc ttctgggcaa tcaggaactt tcggccaccg tcagagccta
     1381 ttcgagaaat actttgtttg gctacagcac cgacgacatc cgtagtatca ccctgctgaa
     1441 gggagccgat gcctcggtgt atggttcact gggttcgaac ggggtgctgc tgatcgagac
     1501 cgaacaggcc acttccgaca acctggaaac ccgtatttcc ttcagcggtc agtatggtct
     1561 gagttggccg ggacggagct tgccggtctt gaacgtcagt cagtacaaaa attacctgag
     1621 ggagataggc atgacccgct acgacatgat tacggctctt tacaccgatt atcctttttt
     1681 gcagaatgag gagaattatt attcctactt gttcaacaac accaccgact ggagagggct
     1741 ggttcagaac agggctttta tcaccgacaa tacttttcgt gtggaaggag gcgacgaaat
     1801 agccaaatac aacatttctt tcggctacac ctccgaaggc ggtgtgttgg ggcaaactgc
     1861 ttccgatcgt taccatacgc tgatcaactc caatatcatg gtaagccgta acgtggatat
     1921 tttcaccaac gtaggcctgg cctatatcag cagttctttg caggagcaag gcatgaatgc
     1981 gcccactaac gccgtacttt cctcttattt tgccatgcct ctgctcagcc catacatgaa
     2041 agaaccgaac aacaatgtac tggacaggta tgccacctac aacggctgga atgtaaacaa
     2101 caatcccact tatccttacg acaacgtaag taatcctctg gccatagtca ataccataaa
     2161 cgcttcggac aatatatacg atgccaacat aag
//
LOCUS       DAOSTD010000161         2170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_46156_length_2170_cov_16.695981, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000161 DAOSTD010000000
VERSION     DAOSTD010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46156_length_2170_cov_16.695981"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PLI37_07750"
     CDS             complement(<1..890)
                     /locus_tag="PLI37_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOQ58939.1"
                     /translation="MSIELKKNCKYAMLVPTSIGLRITPDNGQPVHSSDVFHLQVTSA
                     ETNVASVSSILGLPVKVLTTFVKGSPFAHYIKANLRSRNMDFEGVDVDQGGPWGYRHQ
                     INIADSGYGSRGPRVWNDRAGEVGRTLNVKDFDLDRIFGQEGVQIVHLSGLIGALSQD
                     TSKFCLELARVAKKYGTRISFDLNHRASFWKGREKELHEIFTEIASISDILIGNEEDY
                     QLCLGIKGPEAGGKDITAKIDGFKEMIKRVKAAFPNASVFANTLREVNHSNSHEWGAI
                     MLEGENWHVIEPREIHVLDR"
     gene            complement(1034..>2170)
                     /locus_tag="PLI37_07755"
     CDS             complement(1034..>2170)
                     /locus_tag="PLI37_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOQ58940.1"
                     /translation="IDLTRSQEVLVEKGNTFAFRLLGQVNLEESKENFCISPFSVSQA
                     LSMTLNGAAGNTATEMISALGFEGYTQSEINVYQQKMNQALLSLDPSTNIGIANSIWI
                     REDFAVRKEFVDTNTYYFDAEVQNLPFDAAALNAINNWCDVKTKGRIDKILDGIDPDD
                     IMFLLNALYFKGIWVHKFDKTKTEEADFYLAGGEKTKVNMMYQKAEFPYYEDETVQVV
                     ELPFGNEAFSMVFVLPRQGVDMDALIETLDAPCWVNWLHRLRETELNLKLPRFNLEYE
                     IGLIPTMSALGMTSAFDPQRADFSLINADQDLYIGLLKQKTFVKVDEEGAEAAAVTIV
                     GIKLTSAPLDEIHMTLDRPFLFVIKEKSTSCVLFMGRIGNPQDK"
BASE COUNT          572 a          516 c          479 g          603 t
ORIGIN      
        1 atgcggtcca atacatgtat ttcgcggggc tcaatcacat gccagttttc gccttcgagc
       61 ataatggcac cccattcgtg gctgttgctg tggtttactt cgcgcagggt gttggcaaaa
      121 accgaagcat tggggaaggc ggccttgacg cgcttgatca tttccttgaa tccgtcaatt
      181 ttggcggtaa tatctttgcc tccggcttcg gggcctttga tgcccagaca aagttggtaa
      241 tcttcttcgt tgccgatcag aatatccgat atggaagcaa tctcggtaaa tatctcgtgc
      301 aattcctttt cgcggccttt ccagaacgaa gcccggtggt tcaaatcgaa agaaatgcgg
      361 gtgccgtatt ttttggccac gcgggcaagt tccaggcaga atttgctggt atcctgcgac
      421 aaagcgccta tcaggcccga gagatgtaca atttgtacgc cttcctgtcc aaaaatcctg
      481 tcgagatcaa aatctttcac gttcagtgtg cgccccactt cgccggcacg gtcgttccag
      541 accctgggtc cccgcgaacc gtaaccgcta tcggctatat taatctgatg acgatagccc
      601 caggggcctc cctgatctac atccactcct tcgaaatcca tgttgcggct gcgcaggttg
      661 gctttgatgt agtgagcgaa gggactgcct tttacaaaag tggtcagcac ttttaccggc
      721 agaccaagga tagaagaaac actggccaca ttcgtttcgg cactggtaac ctgcagatga
      781 aaaacatcac tgctatggac gggctgcccg ttgtcgggcg taatacgcaa acctatgctg
      841 gtaggaacaa gcatggcata cttgcaattt tttttaagtt cgatactcat aatttactgg
      901 ttatttaaaa tataatagaa tgtttgcttc ttctgagcga atgagctaca aatataaaaa
      961 aagcggagga attttagcag taaatcctcc gcttttaatt atcggaaaag ctcaagggct
     1021 tataatcaat gccttattta tcttgaggat ttccgattct acccataaac agaacacaag
     1081 aagtgctttt ttcttttata acgaacagga aaggcctgtc tagtgtcata tgtatctcat
     1141 ccagcggggc agaggtcagt tttatcccca ctatcgttac ggcagcggct tcggctcctt
     1201 cctcgtctac tttgacaaag gttttttgct tcaacaagcc tatgtacaaa tcctgatctg
     1261 cattgatcag actaaaatcg gccctttggg gatcgaaagc cgatgtcata cccaaggcag
     1321 acatggtagg tatcagtcct atttcatatt ccagattgaa acggggcaat ttaagattaa
     1381 gttccgtttc cctgagacga tgcagccagt taacccaaca aggagcgtcg agggtttcaa
     1441 tcaatgcatc catatcaaca ccctgacggg gcaaaacaaa taccatactg aaggcctcat
     1501 tcccgaaggg cagttccaca acctgaacgg tctcgtcttc gtaataagga aattccgctt
     1561 tttgatacat catattcacc ttggtctttt cgccgccggc caaataaaaa tcggcttctt
     1621 cggttttggt tttatcgaat ttatgtaccc aaatgccttt gaaataaagg gcgttaagca
     1681 aaaacataat gtcgtccgga tcaataccgt cgaggatttt atcgattcga cccttggttt
     1741 ttacatcgca ccaattgttt atggcattta gagcagctgc atcaaaggga agattttgca
     1801 cttcggcatc gaagtaatag gtattggtat cgacaaactc cttgcgcacg gcaaaatctt
     1861 cgcgaatcca aatggaattg gcgataccta tgttggtcga agggtcaaga ctcaataaag
     1921 cctgattcat tttttgctga tagacattga tttcgctttg agtatagcct tcgaaaccca
     1981 gggcagaaat catttcggta gcagtattac cggctgctcc gtttaaagtc atcgacaaag
     2041 cctgcgaaac gctgaagggc gaaatgcaaa agttctcttt cgactcttcc agatttacct
     2101 gccccaacaa acggaaggca aaagtgttgc ctttttccac caacacttcc tgactacggg
     2161 ttaaatctat
//
LOCUS       DAOSTD010000162         2145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_46897_length_2145_cov_17.843062, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000162 DAOSTD010000000
VERSION     DAOSTD010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46897_length_2145_cov_17.843062"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="PLI37_07760"
     CDS             complement(<1..1319)
                     /locus_tag="PLI37_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOQ58941.1"
                     /translation="MINQELINPQSIVVVGGSNNVHKPGGRIVRNLIEGKYRGELYTV
                     NQKGEGDIQGVKNFKDARDIPEVELAILSIPAAACPAMVEILAKEKKVKAFIVISAGF
                     GEETKAGAVLEEQLLKIVEETETALIGPNCIGIMNMNYHGVFTQPVPEFHSEGVDFIS
                     SSGGTALFIIESALMKGLRFSSVWSVGNSKQNGVEDVLEYMDLNFDPLRDSKIKMLYV
                     EQIKDPDKMLYHASSLIKKGCRIAAIKAGSSDSGKRAASSHTAAIASSDSAVEALLRK
                     AGIVRCYSREELTTVAGIFTLKEAKGKNCAIITQAGGPAVMLADALSKGQINVPLLEG
                     EKVEELKTKLLPGAAVGNPIDLIGTGTPEHLATAIDYCEKHFDNINLMMVIFGSPGLV
                     KVYDAYEVLHKKMEECSKPIFPVLPSIVTAGPEVKSFVRKGHVNFSD"
     gene            1503..>2145
                     /locus_tag="PLI37_07765"
     CDS             1503..>2145
                     /locus_tag="PLI37_07765"
                     /inference="COORDINATES: protein motif:HMM:NF017046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HOQ58942.1"
                     /translation="MGNNESPMNYADNTYPFRQDSCFLYYIGLDQPGLIALIDVDENQ
                     TLLFGKEASIDDIVWMGPQSSLAEKALKSGITKSFDIDAIYDILNKAKAQDRTIHFLP
                     PYRADNKLWLSKALNLPDEVLNDLASLSLIKAIVQQREIKSEEEIEEIEKAVDTSVDM
                     HVAAMKTAEPGMTEAQVAAEVHRIALAQGGNISFPIIATINGQTLHNHYHGNIL"
BASE COUNT          577 a          531 c          448 g          589 t
ORIGIN      
        1 tcgtccgaaa aattgacgtg tcccttcctg acaaagcttt taacttcggg gccggccgtt
       61 acaatcgaag gcaggacggg aaaaatcggt ttggaacatt cttccatttt tttgtgcaac
      121 acttcgtagg cgtcgtaaac ttttaccaga ccgggactgc cgaaaataac catcatcagg
      181 ttgatgttgt caaaatgctt ttcacaataa tcgatagccg ttgccagatg ctcgggagtg
      241 cccgttccaa taaggtcgat gggattgccc acggccgctc cgggcagtaa tttggttttc
      301 aattcttcta ccttttctcc ctccagcaaa ggcacattga tctgcccctt ggacaaagcg
      361 tcggccaaca tcacggccgg acctccggcc tgagtaatga tggcgcaatt ttttcctttt
      421 gcttctttga gggtaaaaat accggctact gttgtcaatt cttcgcggct atagcagcga
      481 acgataccgg ctttacgcaa cagggcctcc accgccgaat cgctgctggc gatggctgcc
      541 gtatgtgaag aggccgctct ttttccggaa tcgctactgc cggccttaat agcagctatt
      601 ctgcagcctt tcttgataag tgaggaagca tgatacaaca ttttgtcggg atccttgatt
      661 tgctccacat acagcatttt aattttagaa tcgcgcaagg gatcaaaatt cagatccatg
      721 tattccaaca cgtcttccac tccgttttgc ttactgtttc ccaccgacca gaccgaagaa
      781 aaacgcaaac ctttcatcaa tgcagactca atgataaaaa gggccgtacc tcccgagctg
      841 gaaataaaat ctacaccctc gctatgaaat tcaggaaccg gttgggtaaa cacgccatga
      901 taattcatat tcataatacc tatgcaattc gggccgatca gtgccgtctc ggtttcctcc
      961 acgattttga gcaattgttc ctccaaaaca gctcctgcct tggtttcttc gccaaagccg
     1021 gccgatatca caataaaggc tttgaccttt ttttctttgg caagtatttc aaccatagca
     1081 gggcaggcag cggcgggaat agagagaatg gccagctcca cctccggtat atccctcgca
     1141 tctttgaagt tttttacccc ttgaatatcg ccttctccct tttggttaac ggtatagagt
     1201 tctccccgat atttgccttc gatcaggtta cgcacaatac ggccacccgg cttgtgtaca
     1261 ttgtttgaac ccccaacaac aactatgctt tgagggttga taagttcttg gttgatcata
     1321 gattcaaggt ttattgaact ttccggacaa aggacaaata taattttttt agctgtataa
     1381 attgtctgtc agaaattttt atcggatatt tatccgaaac atatgctatg ttctcttctc
     1441 atacttacat tcaacgacga aacctgcttt gtcacaaatt ggacaaaggt ctggtagtat
     1501 taatgggcaa taatgaaagc cctatgaatt atgccgataa tacttatcct tttcggcagg
     1561 acagctgttt cctgtattac atcgggctgg accagcccgg attaattgcg cttattgatg
     1621 tcgacgaaaa ccaaaccctc ctgttcggca aagaggcgag catcgacgat attgtatgga
     1681 tggggcctca aagcagtctt gccgaaaaag cgcttaaatc gggcattaca aaaagctttg
     1741 atatcgatgc tatttacgac attcttaaca aagcaaaagc tcaggacaga accatccatt
     1801 ttcttcctcc ttaccgtgcc gataataaac tttggctatc caaggctttg aatttgccgg
     1861 acgaagtatt gaacgaccta gcctccctga gtttaatcaa agccatagtt caacaacgcg
     1921 aaatcaaatc tgaagaggaa attgaagaaa tagaaaaagc agtggatact tcggtggata
     1981 tgcacgttgc tgccatgaaa acagccgagc ccggaatgac cgaagcccag gtggctgccg
     2041 aagtacaccg gattgcactt gcacagggcg gaaatatttc tttccccatc attgccacca
     2101 taaacggcca gactttacat aaccattacc acggcaatat cctca
//
LOCUS       DAOSTD010000163         2093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_48555_length_2093_cov_14.718842, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000163 DAOSTD010000000
VERSION     DAOSTD010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48555_length_2093_cov_14.718842"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..1120
                     /locus_tag="PLI37_07770"
     CDS             299..1120
                     /locus_tag="PLI37_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOQ58943.1"
                     /translation="MNKIVSRTELSDLVVRLEVEAPMIAKARKPGHFVILKLGAKGER
                     IPLTISSADTQKGTITLVVQRVGLTSNKLYNMQVGEEITDLLGPLGRATHIENFGTVL
                     CAGGGVGIAPLLPIVEGMKKAGNKVISVLAARSKNLIMLEDEIRRHSDEVVIMTDDGS
                     YGEKGLITVGVEKIIQREKVDLCVTIGPAVMMKFVSLLTKKYDVPTVASLNTIMVDGT
                     GMCGGCRVSVGGKTKFVCVDGPEFDAHQVNFDEMIMRLGTYKEEEQEEMKKLHQK"
     gene            1138..>2093
                     /locus_tag="PLI37_07775"
     CDS             1138..>2093
                     /locus_tag="PLI37_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ58944.1"
                     /translation="MTKEEQIAALRAQTWREDLRKSRKTKERTDIPRVEMNEQDPEIR
                     SHNHLEVNLGLTAEQARLEAARCLDCANPTCILGCPVEIQIPSFVKNIERGDFLEAAR
                     VLKETSALPAVCGRVCPQEKQCEAQCIYTLKLGKPSVAIGHLERFAADYERESGRISV
                     PELAPKNGKKVAVVGSGPAGLSFAGDMAKYGYEVMVFEALHEIGGVLKYGIPEFRLPN
                     SVVDVEIDNLRQMGVQFMTNCIVGRTITIDELKEQGYCGIFVGSGAGLPNFMNIPGEN
                     LIHILSSNEYLTRVNLMDAANPESDTPVAMGKCVAVIGGGNTA"
BASE COUNT          566 a          463 c          571 g          493 t
ORIGIN      
        1 gggctgtcaa tacacgattc atggcatctg caatatcttg cagggaatct atcaaggtgc
       61 cgtccaagtc aaaaataagg gctttaaaag caatcatagg caacgatcgt agaatttgtt
      121 tcaggcctca gtggctgagt ttgcaaagat agatataaat atttgccctt tctactcgct
      181 gtggtacaat caaatttagt acttttgtgc tgatttcgtc cggaacggga ataggctttc
      241 gtgatttctg cgcgtcgagc tctttcggaa atccggactc aacagataaa ctaagcttat
      301 gaacaagatt gtcagcagga ccgaattgtc cgacttagtg gtaaggttgg aggtggaggc
      361 acccatgata gccaaagcca ggaaaccggg ccactttgtt attttaaaac tgggagccaa
      421 aggtgagcga attcccctga ccatttcgtc ggccgatacc caaaaaggca ctattacctt
      481 agtggtgcaa cgagtgggtt tgacgtcgaa caaactgtac aacatgcagg tgggcgaaga
      541 aatcaccgat ttactggggc ctttgggcag ggctactcat atagaaaatt tcggcacggt
      601 gctctgtgcc ggcggaggag tagggattgc ccccttgctg cccatagtcg aaggtatgaa
      661 aaaagccggc aacaaagtaa tctcggtgct ggcggcccgt tccaaaaacc tgattatgct
      721 tgaagacgag attcgccggc attccgacga agtcgtcatc atgaccgacg acggttctta
      781 cggagaaaag ggcttgatta cggttggggt agaaaagatt attcaacgcg aaaaggtaga
      841 tctttgtgta accatcggtc ctgcagtgat gatgaagttt gtgtcgctgt tgaccaagaa
      901 atacgacgtg cccaccgttg cttcgctcaa cactattatg gtagatggca ccggcatgtg
      961 cggaggctgt cgcgtaagcg tgggtgggaa gaccaaattt gtttgtgtgg atggccccga
     1021 attcgacgct catcaggtga attttgacga aatgattatg cgcctgggta cttataaaga
     1081 agaagaacag gaagaaatga aaaaacttca tcagaaatga caagggaaga atcagcaatg
     1141 acaaaagaag aacaaattgc ggctctgcgc gctcaaacct ggcgcgagga cttacgtaaa
     1201 agccgtaaaa ccaaggaaag gaccgatatc ccccgggtag aaatgaacga gcaggatccg
     1261 gaaatccgca gtcacaatca tctggaagtc aatctgggtt taacagcaga acaagctaga
     1321 ctggaggctg cccgttgtct ggattgcgcc aatcctacct gtattttggg ttgtccggtg
     1381 gaaattcaga ttccaagctt tgtaaaaaac atcgaacgcg gcgactttct tgaagcggca
     1441 cgagtgctga aagaaaccag tgcgctgccg gcagtttgcg ggcgggtctg tcctcaggaa
     1501 aaacaatgcg aagcccagtg catctacacg ctcaagctgg gtaagccctc ggtggctatt
     1561 ggtcatctgg agcgttttgc cgccgattac gaacgcgaga gtggccgtat ttcggtgccg
     1621 gaactggcgc ccaaaaacgg taaaaaagtg gctgtggtgg gttcgggacc tgccggactt
     1681 tcttttgccg gcgacatggc caaatacggt tatgaagtaa tggtgttcga agccctgcac
     1741 gaaataggcg gggtgctcaa atacggtatt cccgaatttc gtctgcccaa ttccgtggtg
     1801 gatgtggaaa tcgataatct gcgtcagatg ggcgtgcaat tcatgaccaa ctgcatcgta
     1861 ggcaggacta tcaccataga cgaactcaaa gaacagggtt attgcggtat tttcgtagga
     1921 agcggagccg gtttgcccaa ttttatgaat attcccggcg agaaccttat tcatattctc
     1981 tcgtccaacg aataccttac ccgggtcaat ctgatggatg ccgccaatcc cgaatccgat
     2041 acgccggtgg ctatgggcaa gtgcgttgcc gtgataggcg gtggtaatac cgc
//
LOCUS       DAOSTD010000164         1974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_52873_length_1974_cov_8.697759, whole genome shotgun sequence.
ACCESSION   DAOSTD010000164 DAOSTD010000000
VERSION     DAOSTD010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52873_length_1974_cov_8.697759"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLI37_07780"
     CDS             <1..311
                     /locus_tag="PLI37_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HOQ58945.1"
                     /translation="NNIRDMETDKINRITLPLVLGKPKAKIYHHLLVYGAFVLILIYN
                     LLKGLAWPQYLFLLLLPLAAFHLNRVRALNDEALDRQLPVLVLLTILFSLIVGFTSFL
                     "
     gene            complement(295..1005)
                     /gene="ubiE"
                     /locus_tag="PLI37_07785"
     CDS             complement(295..1005)
                     /gene="ubiE"
                     /locus_tag="PLI37_07785"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOQ58946.1"
                     /translation="MPKKKAVKHLFDRIAPYYDRLNHLLSLHVDKIWRRKAVKKVLVV
                     PHERVMDMACGTADFSLALAKAGVPSVLGLDISEQMLRIGRQKIQSQGLSHIIQLEQG
                     DSEAIAYPDKSFDVVTVAFGVRNYENLELGLQEMLRVLKPGGMLLVLELSLPQSRFLY
                     ALYSFYFTRILPWIGGLISGDKQAYAYLPASVLRFPKPKEFLQIMQSCGCVETRQQAL
                     SLGLARIFTGKKQITGKK"
     gene            complement(1011..1652)
                     /locus_tag="PLI37_07790"
     CDS             complement(1011..1652)
                     /locus_tag="PLI37_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58947.1"
                     /translation="MIRRLFIPAVFFLSINYSCFAGENGTKFGYRGFSGGMMPHSGYF
                     WGGKTTLRDAQGAVLAVEEMRGIPLGLGGAIRFHFGDHFRVGAEGYGTTLRYGNYNSN
                     VSIGWGGLLAAYYCEYGKFKPFAGATLGGGSVKNLTLLQDNPLDDIAEPAVSYRNYPF
                     MAFCPFTGAEYCLNKKIHLIFKIDYLMNISNPQDDFVRGPRVYFGFVFYHIKR"
BASE COUNT          556 a          464 c          423 g          531 t
ORIGIN      
        1 tgaacaatat ccgggacatg gagaccgaca agatcaaccg catcacgctg ccccttgtgt
       61 tgggcaaacc taaagccaaa atctatcacc atctgctggt atatggggct tttgtgctga
      121 tcctgattta caatctgctt aaaggtttgg cttggcccca atatcttttt ttactgttgc
      181 ttcctctggc ggcttttcac ctgaatcgtg tgcgggcgct caacgatgag gcgctcgata
      241 ggcaactgcc ggttctggtg ctgctgacca tcctgtttag tctgatagtg ggatttactt
      301 ctttcctgta atctgcttct ttcctgtaaa aatacgggcc aatcccaggc tcagagcttg
      361 ctgccgggtt tcgacacagc cgcaggattg cataatttgt aggaattcct tgggtttagg
      421 aaaacgcaga acggaagccg gcaaataagc ataggcttgc ttatcgcccg atatcagccc
      481 gcctatccaa ggtaaaatgc gggtgaaata aaaagaatag agagcataca aaaatcgact
      541 ctgaggcaga gaaagttcca aaaccaaaag cattcctccc ggtttgagta cccgcagcat
      601 ctcttgcaag cccagctcta agttttcgta attgcgtaca ccgaaagcca cggttacgac
      661 atcaaaactc ttgtcgggat aagcaatggc ttcgctgtcg ccctgttcca attgaataat
      721 atgactcagg ccctgagact gaatcttttg ccggccgata cgcagcattt gttccgaaat
      781 atcgagccct aacaccgagg gaacaccggc cttggccaga gcgaggctga aatcggccgt
      841 tccgcaagcc atatccatta cccgttcgtg aggaaccaca agaacctttt tgacagcctt
      901 acgtctccaa atcttatcca catggagcga caacaaatga ttcaagcggt cgtaatacgg
      961 ggcaatccgg tcgaataaat gcttaacggc tttctttttg ggcatgaagc ttagcgtttg
     1021 atgtgataga acacaaaacc gaaatagact ctggggcctc gcacaaaatc atcctgggga
     1081 ttggaaatat tcatcagata atcgatcttg aagatcagat gtattttttt gttcaggcag
     1141 tattctgctc ctgtaaacgg acagaaagcc ataaaaggat agtttctgta ggatacggcc
     1201 ggttctgcaa tatcatcgag aggattgtcc tgcaataaag tcagattttt gaccgaacca
     1261 cctcccaggg ttgctccggc aaaaggtttg aatttaccgt attcgcaata gtaagcggcc
     1321 aaaagacctc cccagcctat ggatacattg ctgttgtaat ttccgtaacg gagcgtagtg
     1381 ccgtatccct cggcgccaac ccgaaaatga tctccgaaat gaaagcgaat ggctccgccc
     1441 aagcccaggg gtattcctct catttcttcc acagcgagaa ctgctccctg agcatcgcga
     1501 agtgttgtct ttccgcccca aaaatacccg ctgtgaggca tcataccacc ggaaaaaccc
     1561 cggtatccga attttgttcc gttttcgccg gcaaagcaac tatagttaat actcaagaaa
     1621 aaaacagccg gtataaatag tcttcgaatc attgtatcaa tagccgaact taaaagtata
     1681 aaggccggtc tcttttaaaa aaacgcaaag gtaagcattc ccggaaatac taaaaaagca
     1741 agctgcagaa cagtttataa aaataatttt cttcagagaa aaaaattttt ataaaaaaga
     1801 acaatttgag aatattgtgc gtatcttcgc ggcggttttc aatatccttt tttacttatt
     1861 ttttattaat tacttcaatg aaaaaatcaa ttgctttggt tcttttgttt gctgctcttt
     1921 ttctgatctc ttgtcagcaa aaaggcggtt ctaaagttga cactacagtt gtcg
//
LOCUS       DAOSTD010000165         1944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_54039_length_1944_cov_13.907888, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000165 DAOSTD010000000
VERSION     DAOSTD010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54039_length_1944_cov_13.907888"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1667)
                     /locus_tag="PLI37_07795"
     CDS             complement(<1..1667)
                     /locus_tag="PLI37_07795"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HOQ58948.1"
                     /translation="MASLAGVLLFASLAFWLLGFYKAELLYRGQELNLFLFDGTFFLN
                     HFTVPGGLLEYAGGFLSQFFYYPLWGALLMLAVLLLIQRTVYRVLSLPRDCFLLSFLP
                     SCFLLLYVTQLDYNIYVLKSGDVFYSQMLGFLFALLPLAVRKLVAQRSNVLHYGLMLL
                     YLVVGYPLAGFYALLGMLYLLIYHVSHLNLKGSKSKTVPVFIITGLVLLLSVPPIYSF
                     FYTNINPVYLYAYGLPVNDFYGSGSMYLPLLSAWLSFGLFLVFLSKPLKELKTLAFSG
                     VFALLLLSMGVVWVFTNKDKNLEAKLAIENAISRDDWNKVLSLSTAAHQNPDRIMIMY
                     RNLALWKKKSLCQTMFLYPQGDAALQTGNKFVNQTRIAAQTVTLYYGMVNYCYRWNME
                     NAVSFGFSIHNLKYMLRASILNREEALAGKYAHILSKTLFYKQWAQDELYYLENPRKM
                     REHPVYADILALRGFSNSFAADPEVMESAIHDHFMSLKPNSLPPMELKMSSIMTTRAV
                     HLFWDAFFKYLDKNWTLPLHVQETALMVGHVEERDMPKVLPHFEPAVV"
BASE COUNT          640 a          437 c          420 g          447 t
ORIGIN      
        1 acaacggcag gttcaaaatg tggaagcact ttgggcatat ccctttcctc cacatgacca
       61 accatcagag ccgtctcctg cacatgcaag ggtaaggtcc agtttttatc gaggtatttg
      121 aaaaaagcat cccaaaacaa atgtacggcc cgggtagtca taatactcga cattttcaac
      181 tccataggtg gcagagagtt gggcttcaac gacataaaat gatcgtgtat ggccgattcc
      241 atcacttctg gatcggccgc aaaggaatta ctgaagcccc tcagcgccaa aatatcggcg
      301 taaacgggat gttccctcat tttgcgggga ttctccaaat aatacaactc atcttgggcc
      361 cattgcttgt aaaacagggt tttggacaag atatgagcgt atttccctgc caaggcctct
      421 tccctgttca ggatggaagc tctcagcata tattttagat tatgaatact aaagccgaaa
      481 ctaacggcat tttccatatt ccagcgatag caataattga ccatgccgta ataaagcgtc
      541 acagtttgag cagctatacg ggtttgattg acaaacttgt tgccggtctg caaagctgca
      601 tctccctgag gatacagaaa cattgtctga cagagggatt ttttcttcca cagggccaga
      661 ttgcgataca taatcatgat tcggtcgggg ttctgatggg cggctgtgct caaagagagt
      721 actttgttcc agtcgtctcg cgaaatggcg ttttctatgg ccagcttagc ttccaagttt
      781 ttgtctttat tcgtaaaaac ccacacaaca cccattgaaa gcagcaacag ggcaaaaaca
      841 cccgaaaaag caagggtttt tagttctttc aaaggtttgg acaagaagac aagaaacagg
      901 ccaaaactca accaggccga cagtaagggc agatacatac tgccggatcc gtaaaaatca
      961 tttaccggca aaccgtaagc gtacaaataa accggattaa tattggtata gaagaaagaa
     1021 taaatcggcg gaaccgacag caagagcact aaacctgtga taataaaaac cggcaccgtt
     1081 ttacttttag aacctttaag gtttaaatgg cttacatggt aaatcaaaag atataacatt
     1141 cccagcaggg catagaaacc ggccaaaggg tagcctacaa caagatagag caacatcagg
     1201 ccgtaatgca acacattgga cctttgggca acgagcttac gaaccgccaa aggcagcaaa
     1261 gcgaacaaaa agcccagcat ttgggaataa aaaacatctc cgcttttcag cacgtatata
     1321 ttgtaatcga gctgagtaac gtagagtaat aagaaacagg agggtaaaaa ggaaagcaaa
     1381 aaacaatcgc ggggcaaact caatacgcga tacacagtcc gttgaatcag caataacacc
     1441 gccagcatta gtaaagctcc ccataaagga taataaaaaa actggctcaa aaaaccaccg
     1501 gcgtattcca gcaaaccgcc gggaacagta aaatgattca gaaaaaaagt accgtcgaac
     1561 aagaataaat tcaattcttg ccctctgtac aacaactcgg ccttgtaaaa gcccaaaagc
     1621 cagaacgcca gagacgcaaa caaaagaact ccggcaaggg atgccatctt tcgggccgcg
     1681 gatattttag ctcgctcgac gttcatttag ggataaaacc agggttcaag ttgtttaaga
     1741 tttagaaagg aaggatgcct caacttgcaa ggcatccttc cggtgtagtg tcttcccctt
     1801 cagaaaaaag aggaacaaat catggaatta tgctcaacga acaagaacct tagcggcctt
     1861 gttgccggct tttacaatat agatgccttg tttgagctgt tcggcaggat taatctgatg
     1921 tcccagaata gaatatacct ggta
//
LOCUS       DAOSTD010000166         1938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_54278_length_1938_cov_7.557621, whole genome shotgun sequence.
ACCESSION   DAOSTD010000166 DAOSTD010000000
VERSION     DAOSTD010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54278_length_1938_cov_7.557621"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1938
                     /gene="sprA"
                     /locus_tag="PLI37_07800"
     CDS             <1..>1938
                     /gene="sprA"
                     /locus_tag="PLI37_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOQ58949.1"
                     /translation="SGVVVNKVEVWVTNKTAVMDNARDILAFADMAEPERIYNAYWTK
                     GQNNYPSNDANNLYVEMVENYAEARQINVSATVLEPLAAQGIIIGNDYERLESARRLE
                     PTEYKLNASLGYISLNQALRSDEVLAVAFEYTKNGRVYQVGEFSTDGIESPKALLVKL
                     LKGTDFSPRAPYWDLMMKNVYALGAYSMSKDNFKLNISFQNDTLGLYVNYISEGKIAN
                     QILLRVMNLDRLNLNRDPIPDGQFDYVEGYTVMSSNGRIIFPVLEPFGSHLRKAIGDD
                     ALAEKYIFQELYDSTLTVVQQLTEKNKFRIEGEYKSSQGGADISLGTMNVTRGSVKVT
                     AGGILLTENVDYVVDYNSGTVSIINENLLSSGSTIHATCEDQGIYNMVRKSLAGFNME
                     YAFSKDFSVGATLMHLSEKPLTNKVDMNTEPLNNTIMGLNTGFTVQSQGLTNLIDKLP
                     LINVTKPSQLTVKAEVAQLKPGASKQIDNTVYVDDFETAKKTFSLRDVTQWFLASTPY
                     DPMGGLFPEAAYSNDLRYGMNRALMSWYTIDQIFTQSRSQTPDHIRNDYEQLSNHYVR
                     AVNEKEIYPDRDLQFNQTGLLSVMNISYYPKQRGPYNFDTEGMQADGTLANPEQRWGG
                     IMRRIESSMTNFEAANVEYIE"
BASE COUNT          535 a          450 c          478 g          475 t
ORIGIN      
        1 cctcgggcgt ggttgtaaac aaagtggaag tctgggtgac caataaaacg gcggttatgg
       61 acaatgcccg cgacattctg gcttttgccg atatggctga gccggaacgg atatacaatg
      121 cctattggac aaaagggcag aacaattatc cttcgaacga tgccaacaac ctctatgtcg
      181 aaatggtgga aaattacgcc gaagccaggc agatcaatgt ttctgcaacg gtgctggaac
      241 cattggccgc ccaggggatc attatcggca acgattatga acgcctggaa agtgcccgcc
      301 gtcttgaacc caccgaatac aagctcaacg ccagtttggg ttatatcagc ctgaatcagg
      361 ctttgcgttc cgacgaagtg ttggccgtgg cttttgaata taccaaaaac ggcagggttt
      421 accaggtggg tgaattttcg accgatggga ttgagtcgcc caaggcgctg ctggttaagt
      481 tgctcaaagg taccgatttc tcgccgagag ctccctattg ggacttaatg atgaaaaacg
      541 tatatgcctt gggggcttac agtatgtcga aagataattt caaactgaac atcagcttcc
      601 aaaacgatac gctgggattg tacgtcaact atataagcga aggaaagatt gccaaccaga
      661 ttttattgag agtgatgaac ctcgaccgcc tgaacctgaa tcgcgatccc attccggacg
      721 gacagttcga ctatgtggag gggtatacgg tgatgtcgtc caacggccga atcatttttc
      781 cggtgctgga gccttttggt tctcacttgc gcaaggccat aggcgatgat gccttggctg
      841 aaaaatacat ttttcaggaa ctctacgatt caactctgac tgttgttcag cagctgaccg
      901 agaaaaacaa atttcgtatc gaaggggagt ataagtcttc tcagggagga gcggatattt
      961 cgctgggcac gatgaatgtc acccggggtt cggtcaaagt aacggccggt ggaatcttac
     1021 tcaccgaaaa cgtcgattac gtggtggact ataattccgg aacggtgagt attatcaacg
     1081 aaaatctgtt gtcttcggga agcaccatcc atgccacctg cgaagatcag ggcatctaca
     1141 atatggtgcg taaatccctg gccggcttca atatggaata cgctttcagt aaggatttca
     1201 gtgtgggagc caccttgatg catctttccg aaaaacctct taccaacaag gtagacatga
     1261 ataccgagcc cctcaacaac accatcatgg ggcttaacac cgggtttacc gttcagtcgc
     1321 agggcctgac caacctgatt gataaactgc ccctgattaa cgtgactaaa ccttctcaat
     1381 taacggtcaa ggccgaagta gctcaattga agccgggtgc ctccaaacaa atagacaata
     1441 ctgtttacgt ggacgatttt gaaaccgcca aaaagacttt cagcctgaga gatgttactc
     1501 aatggttttt ggctagtact ccttacgacc ccatgggagg actgtttccc gaagctgcct
     1561 attccaacga tttgcgctat ggcatgaacc gtgcactgat gtcctggtac actatcgatc
     1621 agatatttac tcaaagccgt tctcaaaccc ccgatcatat ccgcaacgat tatgagcaat
     1681 tgtccaatca ctatgtgcgg gcggtcaacg aaaaagaaat ctatcccgac cgtgatttgc
     1741 agtttaatca gaccggtttg ttgtcggtga tgaatatttc ttattacccg aaacaaaggg
     1801 ggccgtataa tttcgatacg gaaggcatgc aagccgatgg tactctggcc aatcctgaac
     1861 aacgctgggg aggaatcatg cgtagaatag aatcgagcat gaccaatttc gaagcagcca
     1921 atgtggaata cattgagt
//
LOCUS       DAOSTD010000167         1901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_55777_length_1901_cov_11.292524, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000167 DAOSTD010000000
VERSION     DAOSTD010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55777_length_1901_cov_11.292524"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLI37_07805"
     CDS             <1..188
                     /locus_tag="PLI37_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HOQ58950.1"
                     /translation="YYGVYVPKGVWREMNNFSTNSVALIMSSTPYSKADYIYNYEEFL
                     QYAAAKTQSGPDKPKQV"
     gene            185..610
                     /locus_tag="PLI37_07810"
     CDS             185..610
                     /locus_tag="PLI37_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HOQ58951.1"
                     /translation="MKNTVYDCSFIELPRIEFRAGNITTVYGNINIPFKIRRVFYTYD
                     IPGGEARGAHAHKECHQFLVAAGGSFEVALDDGVNKRTVLLNRPFYGLHIPPGIWASE
                     QGFSSGSVCLVLASTLYDENEYIRDYDEFKAFRKQFEKQ"
     gene            663..1763
                     /locus_tag="PLI37_07815"
     CDS             663..1763
                     /locus_tag="PLI37_07815"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOQ58952.1"
                     /translation="MVKYLDLKKITAKYAKEIHDAVARVVDSGWYLQGEENKRFEQHY
                     AEYIGTRHCVGVANGLDALIWILRAYKEMGFMKAGDEVLVPANTYIASILAITDNGLK
                     PVLIEPRLSSYQIDEDLIEKHITERSKALMIVHLYGQCAYSDKIKALCSHYRLKLIED
                     NAQAHGCLYRGRKTGSLGDAAGHSFYPGKNLGAFGDGGAVTTDDEELASVVRTLANYG
                     SEKKYVFRYTGRNSRLDEIQAAVLDVKLRYLDRDIELRKQVAARYLQEIRHPEIQLPI
                     VTDRDQHVYHIFPVRCGRRDALQSFLDGRGIQTIIHYPIPPHKQACYPEWNKMSFPIT
                     EQIHREELSLPLSPVMTGEEIDRVIDAVNAFH"
BASE COUNT          531 a          441 c          446 g          483 t
ORIGIN      
        1 cttattacgg cgtctatgtt cccaaggggg tttggcgcga aatgaataat ttttcaacca
       61 attccgtggc cttgattatg tcttctactc cctattctaa agcggattat atttataact
      121 acgaagaatt tcttcagtac gctgccgcca agactcaatc cggcccggat aaacctaagc
      181 aagtatgaaa aacacagtct acgattgttc ttttattgaa ttacccagga tagaattcag
      241 agccggaaac attactactg tttacggcaa tataaacata cctttcaaaa tcagaagagt
      301 tttttatacc tacgatattc ccggaggcga agcccgtgga gctcatgctc acaaggaatg
      361 ccatcagttt ctggtggcag cggggggaag ttttgaagtg gctttggacg atggggtaaa
      421 caagcgaact gtgctgctga accggccttt ttacggcctg catattcctc cgggtatttg
      481 ggcttccgaa cagggttttt cgtccggttc cgtttgtttg gtgctggcct ctactcttta
      541 tgatgaaaat gaatacatca gagattacga tgagttcaag gctttcagaa aacaattcga
      601 aaaacaatag tacgatcagg cattcgagtc cgagaaaaat agtttggcat taaaaaatgg
      661 ctatggtaaa atacctggat ttaaagaaga taactgcaaa atacgcaaaa gagatacacg
      721 atgccgttgc tcgggtggtt gattcgggct ggtatcttca gggcgaggaa aacaaacgat
      781 ttgaacaaca ttatgccgaa tacataggaa cccgacattg tgttggtgta gctaacggcc
      841 tggatgcatt aatctggatt ctgagagctt ataaagaaat gggcttcatg aaagccggcg
      901 atgaagtcct ggtgccggcc aacacttaca ttgcctccat actcgctatt accgacaatg
      961 gacttaagcc tgttttgatc gaacccaggc tttcgagtta ccagattgac gaagatttaa
     1021 tcgaaaaaca tattaccgaa cgcagcaaag cgctgatgat cgttcatctc tacggccaat
     1081 gtgcctacag cgacaaaatt aaggccctct gctcgcatta caggcttaaa ctgatagaag
     1141 ataatgccca ggctcacgga tgcctctaca gaggccgcaa aaccggttct ttgggtgatg
     1201 ccgccggtca tagtttttat cccggaaaga atctgggggc ttttggcgac ggaggagccg
     1261 ttaccaccga cgacgaggaa ttggcatcgg tggtacgtac cctggccaat tacggttccg
     1321 aaaaaaaata cgtgttccgc tataccggcc gcaacagccg gctcgacgaa attcaggcgg
     1381 ctgtgctcga tgtgaaactt cgttatctgg atcgggatat tgaactgcgc aaacaagtgg
     1441 ccgcccggta tttgcaggaa atccgtcatc ccgaaataca attgcccatt gtgaccgacc
     1501 gcgatcaaca cgtctatcat attttccccg ttcgttgcgg ccgccgtgat gctttacaat
     1561 cttttttgga cggcagggga attcaaacca ttatccacta ccctattcct cctcataaac
     1621 aagcctgtta cccggagtgg aacaagatgt cttttcccat caccgaacaa attcaccgtg
     1681 aagaactcag tttgcccctg agtccggtaa tgaccggtga agaaatagat cgggtaattg
     1741 acgcagtgaa cgctttccac taaaatgcgc taccatcccc gggacataga aaaaatatac
     1801 gcccgcataa agaggctggc tgaaaaggcc ctccaaaagg gagattttgc ccgtgcattg
     1861 cgggaatacg atcgtgctgc cgtagtggct tccaacttaa a
//
LOCUS       DAOSTD010000168         1867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_57265_length_1867_cov_11.179912, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000168 DAOSTD010000000
VERSION     DAOSTD010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57265_length_1867_cov_11.179912"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PLI37_07820"
     CDS             complement(<1..343)
                     /locus_tag="PLI37_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOQ58953.1"
                     /translation="MLKGKKIVLGITGSIAAYKSAYLVRLLVRKGAEVQVVMTPAARE
                     FITPVTLSPLSGKAVISEFFTANNGLWHSHVDLGLWADLMLIAPATASTIGKMANGIA
                     DNMLITTYLSMK"
     gene            complement(346..1113)
                     /locus_tag="PLI37_07825"
     CDS             complement(346..1113)
                     /locus_tag="PLI37_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOQ58954.1"
                     /translation="MKLNLKNPIVFFDLETTGINPATDRIVEISYLKIHPNGKEESHT
                     YRVNPGIPIPKEASEIHHITDEMVAGCPGFKEVGQIIANDIKGCDLAGFNSNRFDVPL
                     LAEEFLRNDIDLDMSARKLIDVQVIFHKMEQRNLKAAYRFYCDKELEDAHSAEADTRA
                     TYEVLQAQLERYPELQNDVEFLAEFSTQNRNVDFAGKFIYNNKGEEVFNFGKYKDLSV
                     EEVLKKDPGYYGWMMNGDFALNTKNVLTKIRLRSLQK"
     gene            complement(1188..>1867)
                     /locus_tag="PLI37_07830"
     CDS             complement(1188..>1867)
                     /locus_tag="PLI37_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOQ58955.1"
                     /translation="GVVMDIFPDNVTFVASDAHKLVKLTNVSFHGEAPAEGEEAKAST
                     LIIPRKPASILKSILPKESGDVTIVFGENNAMFLMSDYTMLSRLLEGRFPNYNAVIPQ
                     NNPYTVLVDRLAFLNALKRVSVCADPANNLIKLDITNNNILITAQNIDFSTSAEENVE
                     CQYDSEPLSLGFKAVFLIDIISNITTPEILLKLADPSRAGLILPSVNEESEELQMLLM
                     PMMINRS"
BASE COUNT          456 a          425 c          440 g          546 t
ORIGIN      
        1 ctttcatcga aagataagtc gttatcagca tattgtccgc aatgccgttg gccattttgc
       61 ctatggtcga agccgtggcc ggagctatca gcataagatc tgcccataat cccagatcga
      121 cgtgactgtg ccagagcccg ttgttggctg tgaaaaattc gctgatcacc gctttgcccg
      181 acaaagggct caaagtaacc ggcgtaataa attctcgggc ggcgggagtc attaccacct
      241 gcacttcagc tcctttacgt acgagaagcc tcactaagta ggccgatttg taggcagcta
      301 tgctcccggt aatgcccagt acgatttttt ttcctttcaa catgtttatt tctgcaaaga
      361 acgtaatctg attttggtaa gcacattctt ggtgttaagg gcaaaatctc cgttcatcat
      421 ccaaccgtaa tagccgggat cctttttcaa cacttcttcg acggaaagat ccttgtattt
      481 gccaaaattg aaaacttctt cgcccttatt gttgtaaata aatttaccgg caaaatctac
      541 gttgcggttc tgggtagaaa attcggccag aaattccacg tcgttttgca attcggggta
      601 acgttccaac tgagcctgga gtacttcgta ggttgccctt gtgtcggctt cggcactgtg
      661 agcgtcttcc agttccttgt cgcaatagaa acgataggcg gctttcaggt tgcgttgttc
      721 cattttgtga aaaatcacct gtacatcgat cagtttgcgg gcactcatgt ccaggtctat
      781 atcgttgcgc aaaaattctt cggccaaaag gggaacatcg aaacgattgg aattaaatcc
      841 ggccaaatca caacccttga tgtcgttggc aattatctgt ccgacttctt taaaaccggg
      901 acagccggcc accatttcat cggtgatgtg atgaatctcg ctggcttctt tgggtatagg
      961 aataccgggg tttaccctgt atgtatgcga ttcttctttc ccgttgggat gtattttcaa
     1021 ataggagatc tcgactatcc tgtcggtagc gggattaata ccggtcgttt ccaaatcgaa
     1081 aaaaacgatg ggatttttta agttcagttt catgttttac agtttgtagc gattcatttc
     1141 ctccggctgc caaagggttg aaaagcttaa tttgccggtg ttcccgccta gcttctgttg
     1201 atcatcatag gcatcagcag catctgtagt tcttcgcttt cttcgtttac ggacggtagt
     1261 atcagtccgg ctctggaagg atctgccagc ttcagaagga tttcgggcgt agtaatattg
     1321 gaaataatgt caatcaaaaa tacagcctta aatcccaagc tcagcggttc gctgtcgtac
     1381 tggcattcca cgttttcttc ggcagaagtg gaaaaatcga tgttttgggc tgtgataagt
     1441 atgttgttgt ttgttatatc cagtttaatc aggttattgg ccggatcggc acaaaccgac
     1501 acccgcttaa gggcattcag gaaagcgagg cgatcgacca gcaccgtata aggattgttc
     1561 tgaggaataa ccgcattgta attggggaaa cgtccttcga gcagacggct caacatggtg
     1621 taatccgaca tcaaaaacat agcgttgttc tcgccaaaga cgatggtaac gtctccgctt
     1681 tccttgggca gtatgctttt taagatcgag gcgggttttc tgggaatgat caatgtggaa
     1741 gccttggctt cttcgccctc ggccggtgct tctccgtgga aggagacatt ggtcagtttg
     1801 accagtttat gggcatccga agccacaaaa gtgacattat cgggaaaaat atccatcact
     1861 accccat
//
LOCUS       DAOSTD010000169         1800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_60412_length_1800_cov_18.495702, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000169 DAOSTD010000000
VERSION     DAOSTD010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60412_length_1800_cov_18.495702"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /gene="murB"
                     /locus_tag="PLI37_07835"
     CDS             <1..1162
                     /gene="murB"
                     /locus_tag="PLI37_07835"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOQ58956.1"
                     /translation="VEGLRNRVWGIHTCRLSRAKLAKNPLIIKNLSYLCDLFLKLMKV
                     QEHFCLKDKHSFHLNVTARYWAEYRNTDELGEILQDKRFNKLPVLPVGGGCNLLFTRD
                     FPGLVIKSAISGWDIVHENEEEVVLRVGSGLDWDQFVESCVSQGWGGIENLSGIPGLT
                     GATPIQNIGAYGVEAKEVIRYVEVFDRQTRSLLVLPVEECGLAYRNSRFKQEWKDRFI
                     VCYITYRLNKKPAYRLDYGNLNEVLRSQGGLQEGPEAHRGNLAMIRRAVLAIRSSKLP
                     DPAVLGNAGSFFKNPELDVATYSALAERFPELPHWELPGNKVKVSAAWMIEYCGWKGK
                     SMGRAGVYPKQALVLVNLGDARGEEIYNLYQHIVADVHKAFGISLQPEVVVL"
     gene            1274..>1800
                     /locus_tag="PLI37_07840"
     CDS             1274..>1800
                     /locus_tag="PLI37_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOQ58957.1"
                     /translation="MKFIASSTTLLGHLQTISRVINPKNTLPALDNFLFKIEDGRLTV
                     TASDAENTLVTTMDLVESDSDGLFCIPAKNMLESLREIAEQPLHFEFTADSMELNIYY
                     RNGQFNFVAQDGMEYPQLRPLKNKLGEIVMPASLLYRGIASTLFAAADDELRPVMNGV
                     VMDIFPDNVTFVASDA"
BASE COUNT          455 a          376 c          460 g          509 t
ORIGIN      
        1 tgtcgaaggc ctgagaaata gggtttgggg catccatacc tgcagattaa gtcgggcgaa
       61 attagctaaa aatcccttaa ttatcaaaaa tctctcgtat ctttgcgacc tgttcctcaa
      121 gctaatgaaa gtacaagaac atttttgctt aaaagacaaa cacagctttc atctgaatgt
      181 gactgcccgc tattgggcag agtatcgtaa caccgatgag ctcggggaaa ttctgcaaga
      241 taaacgtttc aataagcttc cggttttgcc ggtgggcgga ggctgtaatt tgctttttac
      301 ccgcgatttt cccggtctgg tcattaaatc ggccattagc ggttgggata ttgtgcatga
      361 aaacgaagag gaagtcgtgc tgcgggtggg ttccggtctg gattgggatc aatttgttga
      421 aagctgcgtt tcgcagggct ggggaggtat tgaaaacctt tcaggcatac cgggtctgac
      481 aggtgccact cctattcaaa acatcggagc ttacggcgtg gaagccaaag aagtaatacg
      541 ttacgtcgag gtttttgaca ggcaaactcg aagcctgctg gttttacccg tcgaggaatg
      601 tggattggct taccgcaaca gccgttttaa acaggaatgg aaagaccgct ttatagtgtg
      661 ttatataact taccgtctga ataaaaaacc ggcctatcgc ttagattacg gcaatctgaa
      721 tgaagtactg cgttcgcagg gcggcctgca ggaggggcct gaggcacatc gaggcaacct
      781 ggctatgatt cgtcgtgcgg tgttggccat tcgttcctca aaacttccgg atcctgccgt
      841 tttgggcaat gccggcagtt ttttcaagaa tcccgagttg gatgtcgcga cttattctgc
      901 tttggctgaa cgctttcctg agctgccgca ttgggagctt cccggcaaca aagttaaagt
      961 gtcggcagcc tggatgattg aatattgcgg atggaaaggc aagtcgatgg gcagggcggg
     1021 agtttatccc aaacaagctc tggttctggt aaatctgggc gatgcacggg gagaggagat
     1081 ctacaattta taccaacata ttgttgccga tgtgcataaa gcgttcggta tcagccttca
     1141 acccgaagtg gttgtgttat gagattaagt attgataatg tgtagtttaa ttagtttttt
     1201 atttttttca gcctgagctc agggctattc tatttttttc ataactttgt agctcatcta
     1261 aacaatgtcc accatgaaat ttattgcatc aagtactaca ttattgggtc atcttcaaac
     1321 catcagcaga gtcatcaatc ccaaaaacac cttgcctgcg ctggataatt tcctgtttaa
     1381 aatcgaggat ggccggttga cggttaccgc ctcggacgcg gaaaatacgc tggtaactac
     1441 tatggactta gttgaatccg attccgacgg cctgttttgc attccggcca aaaacatgct
     1501 cgaatcgctt agagagattg ccgaacaacc tttgcatttt gaatttacgg ccgacagcat
     1561 ggagttgaat atctactaca gaaacgggca attcaatttt gtggctcagg acggcatgga
     1621 atatcctcag ttgagaccgc tcaaaaacaa gctcggagaa attgtgatgc cggcttccct
     1681 tttgtacagg ggtattgctt ctactctttt tgcagcagcc gatgacgagt tgaggccggt
     1741 gatgaatggg gtagtgatgg atatttttcc cgataatgtc acttttgtgg cttcggatgc
//
LOCUS       DAOSTD010000170         1798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_60502_length_1798_cov_18.382674, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000170 DAOSTD010000000
VERSION     DAOSTD010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60502_length_1798_cov_18.382674"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="PLI37_07845"
     CDS             <1..1485
                     /locus_tag="PLI37_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOQ58958.1"
                     /translation="LFLEEKGYELVTATNGQDALDLCRSQVFDLVFLDENMPGLSGLE
                     TLNAIKELYPSLPIVMITKSEEEDIMDQAIGSKISDYLIKPVNPNQILLTIKKNLHKN
                     EIISGTVTADYQQRFREIALQIEEASGEEDWKEVYKKLVFWELELAGTKNALDEVLLM
                     QKNEANRLFARFVSRHYARWMEGSPDRPLMSPDLFREKVFPLLDEGEKVWFILIDNFR
                     FDQWRMLKPQIGQWFSMDEQLYFSILPTATQYARNAVFSGLYPLQIQQMFPELWVDED
                     DEEGKNLNEAPLIQSQLERYRKKYKFSYHKINDNSAGEKLLANLQSYMDNPLNVVVFN
                     FIDMLSHARTESRMIRELANDEAAYRSLTESWYKHSSMAGLLDYLSQQSCKVILTTDH
                     GTIRVDNPVKVVGDRNTNTNLRYKLGRTLEYNPRQVYEIKNPLQYKLPSPNLSTTYIF
                     AQHNDYFVYPNNYNYYASYYKHTFQHGGISLEEMVVPFILLDSK"
     gene            1482..>1798
                     /gene="tsaE"
                     /locus_tag="PLI37_07850"
     CDS             1482..>1798
                     /gene="tsaE"
                     /locus_tag="PLI37_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOQ58959.1"
                     /translation="MNMLPIEFQLSGIQEAARLLLNNFPDRRVFAFYGGMGAGKTAFI
                     QALCRRLGVKSSVNSPSFALINEYPLDKVDAVYHFDFYRINKLQEAYSIGSEDYFYSG
                     NYC"
BASE COUNT          501 a          442 c          403 g          452 t
ORIGIN      
        1 cttttcctcg aagaaaaagg ctatgagctg gttacggcca ccaatggcca ggacgccctc
       61 gatttgtgcc gcagccaggt ttttgacctg gtttttctcg atgaaaacat gccggggctc
      121 agtggcctcg aaacccttaa cgccatcaaa gaactttatc cctccttgcc catcgttatg
      181 attaccaaga gcgaggaaga agatattatg gatcaggcca taggcagcaa gatctccgat
      241 tacctcatca agccggtcaa tcccaatcag atattgttga ccatcaaaaa aaaccttcac
      301 aaaaacgaaa ttatttccgg gacggtcaca gccgattacc agcagcgttt tagggaaatt
      361 gccctgcaaa tagaagaggc ttcgggcgaa gaagactgga aagaagttta caaaaaattg
      421 gtgttttggg aactggagct ggccggaacc aaaaacgccc tggacgaagt cctgctgatg
      481 caaaaaaacg aagccaaccg gctttttgcc cgttttgtgt cccgccatta tgcccgttgg
      541 atggaaggtt ctccggatcg tcctttgatg agtcccgatt tgtttcgcga aaaagtcttt
      601 cccttgctcg acgagggcga gaaggtctgg ttcatattga tcgataattt ccgtttcgat
      661 cagtggagaa tgctcaaacc tcaaataggg cagtggttca gtatggatga acagctgtat
      721 ttcagtattt tacctactgc tactcaatac gcccggaacg ccgttttttc ggggctttat
      781 cctttgcaga ttcagcaaat gtttccggag ctatgggtag acgaagacga tgaagagggt
      841 aaaaacctca acgaagcgcc cctgattcaa tcccaactgg aacgctaccg caagaaatat
      901 aagttcagct accataaaat caacgataat tcggccggcg aaaaattgtt ggccaacttg
      961 cagagctata tggataatcc gcttaatgtg gtggtgttca attttataga tatgttgtcg
     1021 cacgctcgta ccgaatcgag gatgataagg gaactggcca acgacgaagc ggcttaccgt
     1081 tcactcaccg aatcctggta caagcattct tccatggccg gattgctcga ttatctttcg
     1141 caacaaagtt gcaaagtgat actgaccacc gaccacggca ccatcagagt ggataatccg
     1201 gtcaaggtgg tgggcgatcg caacaccaat accaacttgc gttataaatt gggcagaacc
     1261 ctcgaataca atccccggca ggtatacgaa atcaaaaacc ccctgcaata caaactgcct
     1321 agccccaacc tgagcaccac ctatattttt gcccaacaca acgattattt tgtctacccc
     1381 aacaactaca attactatgc ttcgtactac aagcatactt ttcagcacgg cggtatttcg
     1441 ctcgaagaaa tggtagttcc gtttattttg ttggattcca aataaatatg ttgcccatcg
     1501 aatttcaatt atccggtata caggaggcag ctcgcctgtt gctgaacaat tttccggatc
     1561 gcagggtctt cgctttttac ggcggcatgg gagccggcaa aacagcattt atccaggcac
     1621 tctgccgccg gctgggcgtg aaaagctccg taaacagtcc cagttttgcc ctgatcaacg
     1681 aataccccct cgacaaggtc gatgcggtct atcattttga tttttatcgc atcaacaaac
     1741 tacaggaagc ttacagcata ggcagcgaag actatttcta cagcggaaat tactgttt
//
LOCUS       DAOSTD010000171         1774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_61750_length_1774_cov_16.020942, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000171 DAOSTD010000000
VERSION     DAOSTD010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61750_length_1774_cov_16.020942"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLI37_07855"
     CDS             complement(<1..798)
                     /locus_tag="PLI37_07855"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOQ58960.1"
                     /translation="MDEKYPGKAQPVLVQKASGELQPFDSKKLELSLRNAGAKQETIQ
                     TILDDIQSWVYNGVTTKKIYARAFKMLRGERTAAALRYKLKQAMMEMGPTGYPFEQFI
                     GQIFQKQGYEIQVGVVVEGYSVTHEMDVIATKNGVQLLLECKYSLDQGRHVSVQVPLY
                     VRARVDDIVRKRKEHPLYKDLRFEGGVVTNTRFSADSIQYGRSAGLHLLAWDYPGGKG
                     LKDLIEKYKVYPLTVLTTLTRKEKNYLMEHLVVSCSQLKQQPEWLQEL"
     gene            967..1452
                     /locus_tag="PLI37_07860"
     CDS             967..1452
                     /locus_tag="PLI37_07860"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOQ58961.1"
                     /translation="MKKIVSLFACLFILSLTAGSLQAQNIADKIVGFYTTYDDSGQES
                     SQIQIFKTDDDKYHGKIVWLKEPLENGKPLLDSKNPNKALRNEPIIGLELITGLKYDP
                     KSQEWVNGSIYDPDNGKTYNCFIRFDSNDDTKLNLRGYVGKAWMGLGRTTTWIKGEPK
                     Q"
     gene            complement(1525..>1774)
                     /locus_tag="PLI37_07865"
     CDS             complement(1525..>1774)
                     /locus_tag="PLI37_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ58962.1"
                     /translation="LSLSLISNCINSGGQAVTNNLRVERAIKNITQQQLADELKVSRQ
                     TINAIELGKYVPSTLLALKIARYFQKPLEEIFSLGPED"
BASE COUNT          478 a          437 c          382 g          477 t
ORIGIN      
        1 caactcctgc agccattcgg gttgttgctt caactgactg caactcacca ccaaatgctc
       61 cattaaatag tttttctctt ttcttgtgag cgtggtcaaa actgtgagcg gatatacttt
      121 atatttttcg atcagatctt tgaggccttt tcccccggga taatcccagg ccagcaagtg
      181 caaaccggca gaccgaccat attgtatgga atcggcagaa aaacgggtat tggtcaccac
      241 gcccccttca aaccgcaggt ctttgtacag aggatgctct ttgcgtttgc gaacgatgtc
      301 gtccactctg gcgcgcacat agagcggcac ctgcacgctg acatgccgtc cctggtccag
      361 actgtattta cattcgagta ggagctgcac tccgtttttg gtggcgatca cgtccatttc
      421 gtgcgtcacg ctataaccct cgacaacgac acctacctga atttcgtaac cctgtttttg
      481 aaaaatctgg ccgatgaatt gctcaaaagg atagccggtg ggacccattt ccatcatggc
      541 ctgcttgagt ttatagcgca aggccgcggc ggttcgttcg cctctgagca ttttgaatgc
      601 ccgggcataa attttcttgg tagtgacccc attgtacacc caggattgga tgtcgtccag
      661 gatcgtttga atggtttctt gtttggcccc cgcatttcgc agggaaagtt ccaatttctt
      721 actatcaaag ggttgtaact ctccagaagc tttttgaaca agcacaggtt gcgcttttcc
      781 cgggtatttt tcgtccatta cgcgactgat tttgaaccgt caaaagtaaa aacttttttc
      841 gacttttatt gctcttatca aaaaaggctt atttttgtaa agacacaaaa acctggaaaa
      901 caagggtgtt tcctggttga aacgcaacat attattgttt atcaattgat tataaaaacc
      961 agacttatga aaaagattgt ttcccttttt gcctgcctct ttatattgag tctgacggcc
     1021 ggcagcctgc aagcacaaaa catagccgac aaaattgtcg gtttctatac cacctacgac
     1081 gatagtggcc aagaaagttc acaaatacaa attttcaaaa ccgacgacga caaataccac
     1141 ggcaaaattg tctggctcaa agagcccctc gaaaacggca agcccctact ggacagcaaa
     1201 aatcccaaca aggccttgag aaatgaaccg attatagggc tggaactgat cacgggtctt
     1261 aaatacgatc ccaaatcgca agaatgggtc aatggcagta tctatgaccc cgacaacggc
     1321 aaaacctaca attgctttat aagattcgac agcaacgacg acaccaaact caacctccgc
     1381 ggatacgtag gcaaagcgtg gatgggtctg ggcaggacaa ccacctggat aaagggcgag
     1441 ccgaagcaat aaaactccac gatctgtccg gtgacaaatc ctttgtttcg ggcccgataa
     1501 cttagcttgt accattggag cgctttagtc ctccggacca aggctgaata tctcttccag
     1561 cggcttctgg aaataacggg caattttaag agccaggagg gtcgaaggca catatttacc
     1621 caattcaatg gcattgatgg tctgacgact tactttcaac tcatcggcca gttgttgttg
     1681 cgttatgttt ttgatggcac gctccaccct taaattattg gtcacggcct gtcctcctga
     1741 atttatacag ttcgatatta aacttaatga caaa
//
LOCUS       DAOSTD010000172         1741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_63466_length_1741_cov_10.811388, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000172 DAOSTD010000000
VERSION     DAOSTD010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63466_length_1741_cov_10.811388"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..>1741
                     /locus_tag="PLI37_07870"
     CDS             36..>1741
                     /locus_tag="PLI37_07870"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOQ58963.1"
                     /translation="MKNILNKCLLIALLACLPGLIQAQRVLIRGVVTAYEDKQPLPGA
                     YLLLQNSDGRVVANTITDYEGNYSLLSVIKPGDVLVVSYSGFKKQTIPVENREIINVE
                     MREEILELETATVVGRRQISSGMMSISERDLTTAAVRIEMSELEDLAVASIDDALQGR
                     VAGMDMVTTSGAPGAGMSIRIRGTTSINGSSQPYIIVDGFPYETTISEDFDFSTADEE
                     EYSQMLNIAPGDIKEIIVLKDAAATAIWGSKAANGVLQITTKRGGISPPTVTLGSKGT
                     MSKQAKGIPTLNGDQYVGLILEAMMNSGRTFNPESYPELANDANNPYFYHNYGQNTDW
                     FNELTQTGWTQDHNLAISGGSGKAQYRASVGYYNEEGTTVGTGFERITTRLNVDYNVS
                     EKFRFAANMSYNYSETDRAFISYLRSSYDVLSQAYVRAPNMAVYEYNEAGILTGNFFT
                     PASTLQGYWSGSASSIYNPVAMADESMYKMVSNRIIPNLSVIWNPSSVLRYQFDVGFD
                     VMNNKANAFLPVTATGRPWTESTVNRASDSDSEAFVLQTFNKFLYTPKLGDDHSLQLL
                     AGV"
BASE COUNT          465 a          426 c          445 g          405 t
ORIGIN      
        1 tatccaatta agtgcaaacc atcaacaagg caaggatgaa aaatatactg aataaatgcc
       61 tcttaatcgc tttactggcc tgcctgccgg gattgataca ggctcagaga gtattgatac
      121 gcggagtggt aactgcctac gaagacaagc agcctttgcc gggagcttat cttttgttgc
      181 aaaacagcga cggtcgtgtg gttgccaata ccattaccga ttacgaaggg aattacagcc
      241 ttctgtccgt gataaaaccc ggcgacgtgc tggtagtttc ctattcggga ttcaagaaac
      301 agaccattcc cgtggaaaat cgcgaaatca ttaacgtcga aatgcgggag gaaatcctgg
      361 aactggaaac cgctaccgta gtagggcggc gacagatcag ttcgggaatg atgagcataa
      421 gcgaacgtga tctgactacg gctgccgtca ggatagaaat gagtgaacta gaagacctgg
      481 ccgtggccag tatcgacgac gctttgcagg gcagagtggc cggtatggat atggtgacca
      541 cttcgggagc tcccggtgcc ggtatgtcta ttcgtattcg cggaaccacc tcaatcaacg
      601 ggagttctca accctacatt attgtggatg gtttccctta cgaaaccact atctcggaag
      661 attttgactt ttcaacagcc gacgaagagg aatactctca aatgctgaat attgcgccgg
      721 gagacatcaa agaaataatt gtgctcaaag acgctgcagc cactgctatc tggggctcga
      781 aagccgctaa cggtgtgttg cagattacca ccaaaagggg aggtatcagc ccgccgacgg
      841 tgactctggg ctcaaaagga actatgtcga aacaagctaa aggcattccc acgctgaacg
      901 gtgaccagta tgtcgggctc atcctggagg ctatgatgaa ttcgggcaga acctttaatc
      961 cggaatctta tcccgaattg gccaacgatg ccaacaatcc ctatttctac cacaactacg
     1021 gtcaaaacac cgactggttc aatgaactga ctcagaccgg ctggactcag gaccataact
     1081 tggccatcag cggaggaagc ggtaaagctc agtaccgggc ctcggtgggt tattacaacg
     1141 aagagggaac taccgtaggc accggttttg agcgtattac tacccgcctg aatgtggatt
     1201 acaacgtctc ggaaaaattc aggttcgcgg ccaatatgtc ttacaattac tcagaaaccg
     1261 atcgcgcctt tatttcttac ctgagaagtt cctacgatgt gttgagtcag gcttatgtgc
     1321 gtgctcccaa tatggctgtt tacgagtaca acgaggcagg cattctgaca ggcaatttct
     1381 ttacgccggc ttcgaccctg cagggttact ggtcgggttc ggccagcagt atttacaatc
     1441 ccgtcgccat ggccgacgaa agtatgtaca aaatggtaag caaccgcatc attcccaact
     1501 tatcggtgat atggaatccg tcttcggtgt tgcgctacca gttcgatgtg ggtttcgatg
     1561 ttatgaacaa caaagccaac gctttcctgc cggtgacggc cacggggcgt ccatggacgg
     1621 aatctaccgt taacagggcc agcgacagcg acagcgaggc ttttgttttg caaaccttca
     1681 acaaattttt gtacacaccc aaactgggcg acgatcacag cttacagctt ctggccggag
     1741 t
//
LOCUS       DAOSTD010000173         1709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_65184_length_1709_cov_12.304716, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000173 DAOSTD010000000
VERSION     DAOSTD010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65184_length_1709_cov_12.304716"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="PLI37_07875"
     CDS             <1..1208
                     /locus_tag="PLI37_07875"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOQ58964.1"
                     /translation="ESIDDTYEVEEMNAHHCEAYFLRALSYFYIVRNWREAPLITVPF
                     EDDSHSYNLPKASEAELIEQIKTDIKTALATGAAKEYYETAWETKGRATKWAFFALMA
                     DVCLWSEDYETALTYCDYLLKANSPKAPAFLSLPTHNSWFSMFNPGNSNEAIFELQWN
                     YEEYQTNNLPVLFDNSATDRLYEISFQLLKDFNAEYSHTLENFTEAVRSMYGGYYVAD
                     AYAFEIANAGYVWKYCGSKTLSDKRTHSYYDPNFIIYRVAEVILMKAEALALRSESEQ
                     DWSEAIDLINTIRQRSNLGDFEGQLGPREYSEDLTRETLLEWILYERRMELVGEGKSW
                     YDVLRFGRRHNNAFKSKFLVESVLTYNNQAGQSWLSSVLSNDDALFLPIWEEELKTNT
                     LLVQNPYYY"
     gene            1210..>1709
                     /locus_tag="PLI37_07880"
     CDS             1210..>1709
                     /locus_tag="PLI37_07880"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOQ58965.1"
                     /translation="MKKFSILSIFILLLLSACRDPFLDEPFIKSTGEDLELSNAEFLK
                     KYEDKYSLWIQLLKHADMFNALNDASSVSTVFAPDNRAVEEFLEWRGVESVEELDYEY
                     ARYVAQVHILAQNLPEAMFIAYVETGSIPIKTIFGTYLSTSYGFMDNEVDDVFLPQVK
                     VQDSLS"
BASE COUNT          453 a          399 c          420 g          437 t
ORIGIN      
        1 ccgagagtat cgacgatact tacgaagtgg aagaaatgaa cgctcatcat tgcgaggcct
       61 acttcctgag agccctgagt tatttctaca ttgtgcgcaa ctggagggaa gctcccttga
      121 ttacggtgcc ttttgaggac gattcacata gttacaatct tcctaaagcg agcgaagctg
      181 agctgattga gcaaataaag accgacatta agacggcctt ggctacgggt gcagccaaag
      241 agtattacga aaccgcctgg gaaaccaagg gacgcgccac caaatgggcc ttttttgccc
      301 tgatggccga tgtttgcctt tggtcggaag attacgaaac agccctgact tattgcgatt
      361 atttgttgaa ggccaattcg cccaaggctc cggcctttct gtctttaccc acccacaatt
      421 cctggttttc gatgttcaac ccgggcaaca gcaacgaagc catttttgaa ctgcaatgga
      481 attacgagga ataccagacc aacaatctgc cggtgctttt tgacaactcg gccaccgacc
      541 gtttatacga aatttctttt cagttgttga aagacttcaa cgccgaatac tctcatacgc
      601 tcgaaaactt caccgaagcg gtacgcagta tgtacggagg ttattacgtg gcagacgcct
      661 atgccttcga aattgccaat gccggctatg tgtggaaata ctgcggctcc aagacacttt
      721 cggataagcg tacccacagt tattacgatc ccaatttcat tatctaccgg gtagccgaag
      781 taatattgat gaaagccgag gccctggcac tcagaagtga aagcgaacaa gactggagtg
      841 aagccataga cctgatcaat accatcaggc aacgatccaa tctgggggat tttgagggtc
      901 agttggggcc gagagaatac agcgaagacc tgacacgtga aaccctgctc gagtggattt
      961 tgtacgagcg tcgcatggaa ttggtcggag agggcaaaag ctggtacgat gtgctgcgct
     1021 tcggccgccg ccacaacaat gcctttaaga gcaaattcct ggtcgaatcg gtattgacct
     1081 acaacaatca ggccggccag tcctggctga gttcggtgtt gagcaacgac gacgcacttt
     1141 ttctccccat ctgggaagaa gaattgaaaa ccaatacttt attagttcag aacccgtatt
     1201 actattgata tgaaaaagtt cagtatacta agcattttta tccttttgct tctgtctgcc
     1261 tgcagggatc cctttttgga cgagcccttc atcaaatcga cgggcgaaga cctggagctg
     1321 tcgaatgcgg agtttttaaa gaaatacgag gataaatatt ccctgtggat acagttgctc
     1381 aaacacgccg atatgtttaa tgcgttgaac gatgccagtt ccgtgtcgac ggtgtttgct
     1441 cccgacaatc gggcggtaga ggaatttctg gagtggagag gggtggaaag tgtcgaagaa
     1501 ctcgattacg aatatgcccg ctatgtggct caggtgcata tactggctca aaacctgccc
     1561 gaagccatgt ttattgctta cgtcgaaacc ggttccatac ccattaaaac catttttggt
     1621 acctatttga gtacttctta cggttttatg gacaacgaag tggacgatgt ttttttgcct
     1681 caggtcaaag tgcaggactc tctgtcgat
//
LOCUS       DAOSTD010000174         1685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_66527_length_1685_cov_16.973620, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000174 DAOSTD010000000
VERSION     DAOSTD010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66527_length_1685_cov_16.973620"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PLI37_07885"
     CDS             <1..543
                     /locus_tag="PLI37_07885"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOQ58966.1"
                     /translation="ALHFSIPLKIDKHHNDFKKLKFVNTLLGGYFGSRLMSSLREEKG
                     YTYGISSNINVHRKSSHLDIRCQTDVKYAQAVAQGVYDEIKKLREQEVSGQEMNLVRN
                     HLNGEFARLMDGPFSLSDLYMHTCSLGLDVSYYEEQMEIFQQIPAREVMELAQRYLVP
                     DEFYEVWAGGNNQAAETLIQ"
     gene            555..1451
                     /locus_tag="PLI37_07890"
     CDS             555..1451
                     /locus_tag="PLI37_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005682687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HOQ58967.1"
                     /translation="MSTKQKYMFSKTPKCRLLQFPKIEDDRGNLSFLEAGQQLPFEMA
                     RAYWIYDVPGGEQHGGHAYHTQEECLIALSGSFDVRVRSGRGEKIYSLNRSYRGLYLP
                     AGNWRALENFSTNALGLVLSSGTYDPDEYIRDFSRLKRIRASKKPENLLPIIDDHTGK
                     QVFSVQDSRLVEIPTHHDREGNLTAVNAGRELPFALKRLYYVYDIPYGSQRGGHAHKE
                     LKQWVIAASSSFDILLDDGREQKSFRLDRPNQALEIPAGIWREISGFASGAVVLVLAS
                     AEFSEDDYIRDYEQFLAFRSCN"
     gene            1442..>1685
                     /locus_tag="PLI37_07895"
     CDS             1442..>1685
                     /locus_tag="PLI37_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ58968.1"
                     /translation="MQLVRYQPEYKSCWDTFVAQSRNATFLFFRDYMDYHADRFHDHS
                     LMFYKKDRLLGLLPGNVEGDVYYSHQGLTYGGFLLSF"
BASE COUNT          451 a          378 c          425 g          431 t
ORIGIN      
        1 gccctgcact tttccatccc attgaaaata gataaacacc acaacgattt taaaaaactg
       61 aaatttgtaa atactctcct gggcggttat ttcggcagca gattgatgag cagcctgcgc
      121 gaagaaaagg gctataccta cggcatttct tccaacatca acgtacatcg aaaaagctct
      181 catcttgaca ttcgctgcca gaccgatgta aaatatgcac aggccgtggc gcagggggtt
      241 tacgatgaaa tcaaaaaact gcgggaacag gaggtgagcg ggcaggaaat gaatttggtc
      301 cgcaatcacc tcaacggtga atttgcccga ctgatggacg ggcctttttc cctttccgac
      361 ttgtatatgc acacctgctc tcttggactg gatgtgagct attatgaaga acagatggaa
      421 atttttcagc aaatcccggc tcgcgaagtg atggaactgg cgcaacgtta tctggttccg
      481 gatgaatttt acgaagtatg ggccggaggc aataatcagg ccgccgaaac gttgattcaa
      541 taagccggaa aagagtgtca acaaaacaga agtatatgtt cagcaaaacc cccaaatgtc
      601 ggctactcca attcccaaaa atcgaagacg atcgaggaaa tctgtcgttt ttggaagccg
      661 ggcaacaact gccttttgag atggccagag cgtattggat ttacgatgtt cccggaggtg
      721 aacaacacgg aggccatgcc tatcatactc aggaagaatg ccttattgct ttgtccggga
      781 gttttgacgt gcgggtgcgt tcgggcaggg gagaaaaaat ttattctctg aaccgttctt
      841 atcgaggttt atacctaccc gccggaaatt ggcgcgctct ggaaaacttt tcaaccaacg
      901 cattggggct ggtgttatct tcgggaacct acgatcccga tgaatacatt cgggatttta
      961 gccggttgaa acgaatccgg gcttctaaaa aaccggaaaa tttgttgccg ataatagatg
     1021 atcataccgg caagcaagtc ttttcggtac aggattcccg cctggtagaa atacccaccc
     1081 accacgacag ggaaggcaat ctgacggcag tcaacgccgg tcgggaatta ccgtttgcct
     1141 tgaagcgctt gtattacgtt tacgacattc cttacggcag tcagcgggga ggacatgctc
     1201 acaaggagct gaaacaatgg gtaatagctg ccagtagtag ctttgatatc ttgctcgacg
     1261 acggccggga acaaaagagt tttcgcttgg acaggcccaa ccaggcactg gaaattccgg
     1321 ccggaatctg gagggaaata agcggttttg cctcgggcgc cgttgtgttg gtactggctt
     1381 cggcagagtt ttccgaagac gattatatac gcgattatga acaattctta gccttcagat
     1441 catgcaacta gtccgttacc aaccggaata caaatcctgc tgggatactt ttgtggccca
     1501 atcgagaaac gccactttcc tgtttttcag ggattacatg gattatcatg ccgatcgctt
     1561 tcacgatcat tccctgatgt tttataaaaa ggatcgtttg ctgggtttgt tgccgggtaa
     1621 cgtggagggg gatgtttatt attcacatca ggggcttact tacggcggtt ttttattgag
     1681 cttta
//
LOCUS       DAOSTD010000175         1670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_67418_length_1670_cov_16.658204, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000175 DAOSTD010000000
VERSION     DAOSTD010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67418_length_1670_cov_16.658204"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLI37_07900"
     CDS             complement(<1..611)
                     /locus_tag="PLI37_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58969.1"
                     /translation="MKAANFILTAFLMLLPPAAKAIQDSSLQVHYTFLNEGFTVSDQS
                     GNAYHGQLKNQAVVRSLGTAQVLDLGDRNGYLDMGTDVGTLIAGLEDFSITTAIYIDE
                     KSNITGNGHFVWAFSTSPACGQYTGKYIAYRVNRQRYALSTGGYANEVAALETGTASI
                     KGKWYQLTYTQQGSKGSLYFDSTLVRSSSNLVYLPKDLGQATN"
     gene            871..>1670
                     /locus_tag="PLI37_07905"
     CDS             871..>1670
                     /locus_tag="PLI37_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOQ58970.1"
                     /translation="MITESKKDVIDIIHKVNQHWQAEHPYTVRAFWDNAAYHTGNMEA
                     YFLTGNEAYREYSQKWAEHNEWKGAKSTDKSQWRYHYGENDLYVLFGDWQTCFQTYID
                     LYHIEPEEIKIERAKEVMYYQMSTENRDYWWWSDGLYMAMPVMTKLYKLTGDELFLEK
                     MQEYYQFAEELMYDEEYALFFRDAKYVYPKEPTINGKKNFWSRGNGWVFAAYAKVMQD
                     LPEDNAFRKKIEQRFIAMAASLKKIQQPEGYWTRSLLDPEHAPGPETSG"
BASE COUNT          497 a          423 c          364 g          386 t
ORIGIN      
        1 tagtttgtgg cctgcccaag gtctttgggc aaataaacca gattggacga agaccttact
       61 aaggtactgt cgaaatacag gctgcctttc gaaccctgct gtgtataagt caattgatac
      121 cacttccctt tgatcgatgc cgtaccggtt tccagagcag ccacttcgtt ggcgtagccc
      181 ccggtagaca aggcataacg ctggcggttc accctgtagg ctatgtattt ccccgtatat
      241 tgcccacagg ctgggctggt cgaaaaagcc cagacaaaat gaccgttgcc tgtgatgttg
      301 cttttttcgt cgatgtaaat cgcagtggtg atgctgaaat cttccaatcc cgcaatgagc
      361 gttcctacgt ccgtgcccat gtccaagtaa ccgttccggt cgcccagatc caagacctgt
      421 gccgttccca aactgcgaac gacggcctga ttcttcagtt ggccatgata agcgttaccg
      481 ctctgatcgg acaccgtaaa gccctcgttt aaaaaagtat aatgtacctg gagactggaa
      541 tcctgaatcg ccttcgccgc cggaggcaga agcatcaaaa atgccgtaag aataaaatta
      601 gccgctttca taagacttag ttaaaatcaa cggcaaaaga atggaaaaaa accgggatac
      661 gcaataaaac aaaggtttac aaacattctt tgaacaaaag tttacaccat tatgaatgat
      721 tattagaacc gaataacata gaatttttgt ttcttcgcaa gcgtaattca tttccttact
      781 catatgaaat tttctcccct gacgaagtta tttccgtttg cattttgttt caccctctta
      841 agtttgagtg cttgcgcctc caaaaaagat atgatcaccg aaagcaaaaa agacgtcatc
      901 gacattatcc acaaagtcaa ccaacactgg caagctgagc acccctacac ggtgcgtgcc
      961 ttctgggaca acgccgccta ccacaccggc aacatggaag cttatttcct gacaggcaac
     1021 gaagcctaca gggaatattc ccaaaaatgg gcggaacaca atgaatggaa gggagccaaa
     1081 tcgactgata aaagccagtg gagataccat tacggcgaaa acgatctcta cgtgcttttc
     1141 ggtgattggc aaacctgctt ccaaacttat atcgacctct accacatcga acccgaagag
     1201 atcaaaatag aaagggccaa agaagtaatg tattaccaga tgagcaccga aaacagggat
     1261 tactggtggt ggtccgacgg tctttacatg gctatgcccg ttatgactaa actgtataaa
     1321 ctgacaggcg acgagctctt tctggaaaaa atgcaggaat attaccaatt cgccgaagaa
     1381 ctcatgtacg acgaggaata tgcccttttc ttccgcgatg ccaaatacgt ctaccccaaa
     1441 gagcccacca tcaacggcaa aaagaatttc tggtccaggg gcaacggctg ggtgtttgcc
     1501 gcttatgcca aagtcatgca ggatttgccc gaagacaacg ccttcagaaa aaaaatagag
     1561 caacgtttta tagccatggc cgccagcctg aaaaaaatcc agcaacccga aggctactgg
     1621 acgcgcagcc tgctcgatcc cgaacatgca ccgggccccg aaaccagtgg
//
LOCUS       DAOSTD010000176         1610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_71297_length_1610_cov_14.861736, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000176 DAOSTD010000000
VERSION     DAOSTD010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71297_length_1610_cov_14.861736"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLI37_07910"
     CDS             complement(<1..521)
                     /locus_tag="PLI37_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HOQ58971.1"
                     /translation="MTLYYARGFEVSYFSGYTRVQVFNPWADKEVMARYYLYREDNVS
                     LPKDGQAIRIPLNSLAIASCTHVAFLHLLDELELVKGVCNASMVYNPRLKQAIEEGEV
                     ADLGDFFQINREKLLHIRPQALMASGRNQYDEQLDAISRAGVPVIYNNEWMEQNLLGR
                     AEWLRFVACFLDK"
     gene            complement(709..1032)
                     /gene="secG"
                     /locus_tag="PLI37_07915"
     CDS             complement(709..1032)
                     /gene="secG"
                     /locus_tag="PLI37_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOQ58972.1"
                     /translation="MYLAITILIFIVAILLILIVLVQNSKGGGLASGFASSNQVMGVR
                     KTTDFLEKATWTLVGIVLFGSILSAAFIDRSSTNERQSALKEQVDQAVEMNPNLTAPS
                     FEDEE"
     gene            complement(1038..>1610)
                     /locus_tag="PLI37_07920"
     CDS             complement(1038..>1610)
                     /locus_tag="PLI37_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005832846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ58973.1"
                     /translation="PNRKALYYFMKELPYPPETAGTTGELDFGLVDQFLEEYSGDGDQ
                     LQVEMPDYFQLNTQQSDQAGSKHAPFGKQEDRASSRHTPLGKREESTGSRSEKQEEQD
                     LIEKFLSQPAEHIQIDKSYEEQVPETAPVDEYPEASFTETLAKIYLKQGKYEKALEIF
                     NKLLLKYPEKSIYFADQIRFLNKLIHHLKK"
BASE COUNT          439 a          377 c          361 g          433 t
ORIGIN      
        1 tctttatcca gaaagcaggc tacaaaacgc agccattctg ccctgcccag caaattttgt
       61 tccatccatt cgttgttgta gataacaggc acaccagccc gggaaatggc atctaattgc
      121 tcgtcgtatt gattgcgacc actggccata agagcctgag gacgtatatg aagcagcttt
      181 tcgcggttga tctgaaagaa atctcccaaa tcggccacct ctccctcttc gatggcctgc
      241 ttcaaacggg gattataaac catggaagca ttgcacacgc ccttgaccag ctcaagttca
      301 tcgagcaaat gcaaaaaagc cacgtgggtg caagaagcta tagccagtga atttaggggg
      361 atacggatag cctgtccgtc tttgggcaag gatacgttgt cttccctgta aagatagtaa
      421 cgggccatga cctccttgtc tgcccaggga ttaaacactt gaactctggt atagccgcta
      481 aagtaactga cctcaaaacc acgagcgtaa tacagtgtca ttacggaatc gggattttca
      541 tcgtagaacg caacttgtcc tgccctccgt tgaccacagg agctcaacag cataacgacc
      601 gctaaaaggc aaaaaacaag gtgtttcata aaaacaggcc gcaaaaagaa ataaaaaagg
      661 gagtcaacct gcggatgacg cccttttata gcattttatg gtaagcattt attcttcatc
      721 ctcaaaactg ggagccgtca gattgggatt catttccacg gcttggtcca cctgttcttt
      781 taaagcagac tgacgttcat tagtgctgga gcgatcaata aatgctgccg aaagaatact
      841 gccgaaaagc acaataccaa ccaaagtcca ggtggctttt tccaaaaaat cggtggtttt
      901 gcgcacaccc attacctgat tggaagaggc aaaacccgag gccaatccgc cacccttgga
      961 gttttgaacc aaaacgataa gaatgagaag tatggctaca ataaagatta aaatggtgat
     1021 ggccaggtac atattatcta tttttttaaa tgatgaatca atttgtttag aaatcggatt
     1081 tggtctgcaa agtaaatact tttttccgga tatttcaata acaatttgtt gaatatttca
     1141 agagcttttt cgtatttgcc ctgtttcagg tagattttgg ccagggtctc agtaaaagag
     1201 gcctccggat attcgtcaac cggagcggtt tccggcactt gttcttcgta tgatttgtct
     1261 atttgtatat gttctgcagg ctgactgagg aatttctcga tcaaatcctg ttcttcctgc
     1321 ttttcggacc ggctgccggt tgattcctcc cgcttgccca acggagtgtg ccggctggaa
     1381 gcccggtcct cctgtttgcc gaacggagcg tgcttgctgc cggcttgatc ggattgttga
     1441 gtgttcagtt gaaaataatc gggcatttcg acctgaagtt gatccccgtc gccagagtat
     1501 tcttccagaa actgatcaac caaaccaaaa tccaactccc cggttgtgcc ggcagtttcc
     1561 ggcggataag gcaattcttt catgaaataa tacaaagcct ttctgttggg
//
LOCUS       DAOSTD010000177         1508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2224
            NODE_78532_length_1508_cov_11.211975, whole genome shotgun
            sequence.
ACCESSION   DAOSTD010000177 DAOSTD010000000
VERSION     DAOSTD010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864291
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:31:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,586
            CDSs (total)                      :: 1,558
            Genes (coding)                    :: 1,544
            CDSs (with protein)               :: 1,544
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 0 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78532_length_1508_cov_11.211975"
                     /isolate="AMR_MDS_2224"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PLI37_07925"
     CDS             complement(<1..883)
                     /locus_tag="PLI37_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ58974.1"
                     /translation="MNKPLISKAFNPDNFRHQGHLLVELLGDYLESALQSSTPVNRWR
                     DPEDQLTFWQNDYKEGDRQLEHFFKKLIDSSLHMHNPKNMGHQVAIPAPEAALAGLLT
                     ELLNNGTVVYEIGAPAVVLERVVCKQLAQEMGFDPGKADGFMTSGASLANLTALMAIR
                     NIKAGEDVWNKGNNKQYALMVSEEAHYCIDKSLRIMGLGEAGTIRIPVDEHYKVRVEM
                     LESAYRAARDRGVEVLGIIGCSCSTSTGTYEELEPLADFAQAHNLWFHVDAAHGGPAI
                     FSKKHRHRLKGIERADSV"
     gene            1073..>1508
                     /locus_tag="PLI37_07930"
     CDS             1073..>1508
                     /locus_tag="PLI37_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ58975.1"
                     /translation="MEYKVLSGEYSEIQLATFSPATDNGTREYHALIKLKKVSGLNAS
                     VQLDLLEKAYESLKQEISPATAVWKRYFLSDPCNQYTLFDKPDVAISLVGQPPLDGSK
                     MALWCYFVQEGRVDVDSDVITLTRPAYKHFYYYGLNDLSSGSY"
BASE COUNT          376 a          353 c          353 g          426 t
ORIGIN      
        1 ctacagagtc ggctcgttca atgcctttca aacgatgcct gtgctttttt gagaatatgg
       61 cagggccgcc gtgtgcggca tccacatgga accacaggtt gtgagcctgt gcaaaatcgg
      121 cgagaggttc tagttcttca taagttcccg tcgaagtgga acaggagcaa ccgattattc
      181 ccagcacttc gacaccccgg tcgcgggctg cccggtaagc gctttcgagc atttctacac
      241 gcactttgta gtgttcatcc actggtatgc gaatagtgcc tgcttcgccc aaacccataa
      301 tcctcaaaga tttatctatg caataatggg cttcttcaga caccatcagg gcgtattgtt
      361 tgttatttcc cttgttccag acatcttcac cggccttgat attgcgtatg gccatcaggg
      421 cggtaagatt cgccaacgaa gctcccgaag tcataaagcc gtcggccttg ccgggatcga
      481 agcccatttc ctgagccagt tgtttacata cgacacgctc caacactact gccggagctc
      541 cgatttcgta aactacggtt ccattgttga gcagttcggt caacagcccg gccagtgcgg
      601 cttccggagc aggaattgcc acctgatgcc ccatgttttt gggattgtgc atatgcaaag
      661 aactatcaat cagctttttg aaaaaatgtt ccagttgtct gtcgccctct ttgtaatcgt
      721 tctgccagaa tgtaagctga tcttcggggt ctctccatcg atttacaggt gttgaacttt
      781 gcagggccga ttccaggtaa tcccccaaca attctaccag aagatggcct tgatgtcgaa
      841 agttatcagg gttgaaagct ttggaaataa gcggtttatt cataaattat ctgtttttta
      901 cttaaacccg gctgcccatt tgttggggac cggactttcg ttggtgtaaa cccttggtag
      961 ggaaatgacc tattttggga cgatttttgc ctgataagcc tacaaaggta gtttataaat
     1021 caaaaaatat cctgtatttt tgctttttta aaacaacaac aatagaggta atatggagta
     1081 taaagtactc tcgggagaat acagcgaaat acaattggcc accttttcgc cggccacgga
     1141 caatgggacg cgtgaatacc atgccctgat caaattaaaa aaagtatccg gcctgaatgc
     1201 ttcggttcag ttggatttac ttgagaaagc ctacgaaagt ctgaagcagg aaatttctcc
     1261 ggccacagcc gtatggaaaa gatatttttt gagcgatccc tgtaaccagt atacgctttt
     1321 cgataagccg gacgtagcta tatcgctggt agggcagcct cctttggacg gcagtaaaat
     1381 ggctttatgg tgctattttg tacaagaagg ccgtgttgat gtggacagtg atgtaattac
     1441 tctgaccagg cctgcctaca agcattttta ttattacggg ctgaacgatt tgtcctcggg
     1501 ctcctacg
//
